# Genetic determinism and evolutionary reconstruction of a host jump in a plant virus

Nikon Vassilakos, Vincent Simon, Aliki Tzima, Elisabeth Ida Johansen, Benoît Moury

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Nikon Vassilakos, Vincent Simon, Aliki Tzima, Elisabeth Ida Johansen, Benoît Moury. Genetic determinism and evolutionary reconstruction of a host jump in a plant virus. 16. Rencontres de Virologie Végétale (RVV 2017), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD). FRA., Jan 2017, Aussois, France. 137 p. hal-01605232

HAL Id: hal-01605232

https://hal.science/hal-01605232

Submitted on 2 Jun 2020

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# Genetic Determinism and Evolutionary Reconstruction of a Host Jump in a Plant Virus

Nikon Vassilakos<fnref n="1" /> Vincent Simon,<fnref n="2" /> Aliki Tzima,<fnref n="1" /> Elisabeth Johansen,<fnref n="3" /> and Benoît Moury<fnref n="2" />

In spite of their widespread occurrence, only few host jumps by plant viruses have been evidenced and the molecular bases of even fewer have been determined. A combination of three independent approaches, 1) experimental evolution followed by reverse genetics analysis, 2) positive selection analysis, and 3) locus-by-locus analysis of molecular variance (AMOVA) allowed reconstructing the *Potato virus Y* (PVY; genus Potyvirus, family Potyviridae) jump to pepper (*Capsicum annuum*), probably from other solanaceous plants. Synthetic chimeras between infectious cDNA clones of two PVY isolates with contrasted levels of adaptation to *C. annuum* showed that the P3 and, to a lower extent, the CI cistron played important roles in infectivity toward *C. annuum*. The three analytical approaches pinpointed a single nonsynonymous substitution in the P3 and P3N-PIPO cistrons that evolved several times independently and conferred adaptation to *C. annuum*. In addition to increasing our knowledge of host jumps in plant viruses, this study illustrates also the efficiency of locus-by-locus AMOVA and combined approaches to identify adaptive mutations in the genome of RNA viruses.

<footnote n="1">¹ Laboratory of Virology, Benaki Phytopathological Institute, Kifissia, Greece</footnote>

<footnote n="2">² Inra, UR407 Pathologie Végétale, Montfavet, France</footnote>

<footnote n="3">³ Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej, Frederiksberg C, Denmark</footnote>
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16ièmes Rencontres de Virologie Végétale

15 - 19 JANVIER 2017 • AUSSOIS - FRANCE

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Aussois
du 15 au 19 janvier 2017