Dec 05, 2020

Version 2

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# Easy Covid LAMP V.2

DOI

https://dx.doi.org/10.17504/protocols.io.bqfzmtp6

ahmet¹, ayten¹

¹Hayat

Hayat

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ahmet

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DOI: https://dx.doi.org/10.17504/protocols.io.bqfzmtp6

**Protocol Citation:** ahmet, ayten 2020. Easy Covid LAMP. protocols.io
https://dx.doi.org/10.17504/protocols.io.bqfzmtp6
Version created by ahmet

**License:** This is an open access protocol distributed under the terms of the **Creative Commons Attribution License**, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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**Protocol status:** Working

We use the updated protocol for our colorimetric&fluorimetric assay.

**Created:** December 05, 2020

**Last Modified:** December 05, 2020

**Protocol Integer ID:** 45273

**Keywords:** lamp

Troubleshooting
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1. Collect sample into 1.5ml eppendorf tube.

2. Insert sample transfer plastic into the sample tube, mix for 5 seconds, then insert into pre-filled 0.2ml master mix PCR tube, mix for 5 seconds, discard.

3. Place PCR tube on the pre-heated heat block device and run.

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65 °C (Heat block is pre-set)

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00:29:00

4. Check the color of the PCR tube with naked eye: positive if yellow, negative if pink or orange.

5. Optional: Check the fluorescence signal using handheld fluorescent viewer: positive if bright green, negative if matte green.