# Role of substance P and CGRP in PAR-2-induced rectal hyperalgesia in rats

Bruno Guiard, Nicolas Cenac, Rafael Garcia Villar, Nathalie Vergnolle, Anne-Marie Coelho, Jean Fioramonti, Lionel Bueno

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Bruno Guiard, Nicolas Cenac, Rafael Garcia Villar, Nathalie Vergnolle, Anne-Marie Coelho, et al. Role of substance P and CGRP in PAR-2-induced rectal hyperalgesia in rats. 2001 A Brain-Gut Odyssey, Jul 2001, Oxford, Angleterre, United Kingdom. Digestion, 2001, Digestion. 10.1159/000051897. hal-01604751

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Digestion

Abstracts

2001: A Brain Gut Odyssey

July 11-15, 2001, Oxford, UK

Digestion 2001;63:247-279

https://doi.org/10.1159/000051897

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Digestion

Digestion 2001, 63:247-281

2001: A Brain Gut
Odyssey

Abstracts

July 11-15, 2001, Oxford, UK

Guest Editors

H. Raybould, Davis, Calif., USA

F. Azpiroz, Barcelona, Spain

Contents

Oral Presentations

01-028

Poster Session I

The Brain I

Receptors

Potential I

Feeding I

The Brain I

Poster Session II

Gut to Brain I

The Brain I

The Brain I

Digestion

KAR

Article / Publication De

Published online: June 29, 2001

Issue release date: 2001

Number of Print Pages: 33

Number of Figures: 0

Number of Tables: 0

ISSN: 0012-2823 (Print)

eISSN: 1421-9867 (Online)

For additional Information:
http://www.karger.com/DIG

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Digestion 2001, 63:247-281

2001: A Brain Gut
Odyssey

Abstracts

July 11-15, 2001, Oxford, UK

Guest Editors

H. Raybould, Davis, Calif., USA

F. Azpiroz, Barcelona, Spain

Contents

Oral Presentations O1-O28

Poster Session I

The Brain in the Gut P1-P5

Receptors and Transmitters in the Brain-Gut Axis:

Potential for Novel Therapies P6-P14

Feeding, Obesity and the Brain-Gut Axis P15-P17

The Brain-Gut Axis: Sensations and Psyche P18-P25

Poster Session II

Gut to Brain Signalling P26-P37

The Neuroimmune Brain-Gut Axis P38-P45

The Brain-Gut Axis: Painful Memories P46-P61
ROLE OF SUBSTANCE P AND CGRP IN PAR-2-INDUCED RECTAL HYPERALGESIA IN RATS

Intracolonic infusion to rats of the proteinase-activated receptor-2 (PAR-2) activating-peptide SLIGRL, induced delayed hyperalgesia to rectal distension (RD). This study investigates the involvement of substance-P (SP) and calcitonin gene-related peptide (CGRP) in PAR-2-induced visceral hyperalgesia.

**Methods:** Four groups (n=6-8) of male Wistar rats (250-300g) were equipped with an intracolonic (IC) catheter for SLIGRL (0.2mg/rat) or saline (0.25ml/rat) infusions, and electrodes for recording abdominal contraction (mean±SEM for 5-min episodes) as visceral pain index. RD was performed by 5min steps of 0.4ml (0 to 1.2mL). Groups 1&2 were infused with SLIGRL or saline (0.25mL/20min) respectively, and the NK1-antagonist SR140333 (1mg/kg-IP): first, SR140333 was administered 30min before SLIGRL or saline; second, 3 days later, SR140333 was administered 10h after SLIGRL or saline. Groups 3&4 were similarly infused with SLIGRL or saline respectively, and the CGRP receptor-antagonist human-CGRP<sub>8-37</sub> (100 µg/kg-IV), 20min before and 10h after SLIGRL or saline.

**Results:** SLIGRL (group 1) but not saline (group 2) induced 10h-delayed visceral hyperalgesia to RD (45.0±4.1 contractions vs 33.3±3.0 in basal conditions i.e. 36% increase; p<0.05). SR140333 inhibited SLIGRL-induced hyperalgesia when administered before (45.0±4.1 vs 31.2±4.5; p<0.05) but not after SLIGRL (45.0±4.1 vs 48.0±4.8; p>0.05).

Similarly, SLIGRL (group 3) but not saline (group 4) increased the number of abdominal contractions by 47% in response to RD (1.2mL) at 10h post-infusion. hCGRP<sub>8-37</sub> did not affect SLIGRL-induced hyperalgesia when administered either before or after SLIGRL.

**Conclusion:** NK1-receptor blockade prevents SLIGRL-induced visceral hyperalgesia which suggests that SP, but not CGRP, is involved in PAR-2-induced sensitization of primary afferent neurons.

B. Guiard, N. Cenac, R. Garcia-Villar, A.M. Coelho, J. Fioramonti, L. Bueno
Digestion

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Abrahams, T.P. 259
Adelson, D.W. 268
Aja, S. 263
Akkermans, L.M.A. 277
Al-Chaer, E.D. 254
Altmann, C.A. 254
Avni, Y. 265
Aziz, Q. 254

Babyatsky, M.W. 272
Baffi, M.C. 273
Ball, A.J. 269
Ballinger, A.B. 273
Bantick, S. 254
Barbara, G. 254
Barrachina, M.D. 271
Beckett, E.A.H. 250
Beckmann, C. 254
Bellinger, L.L. 252
Bielanski, W. 270
Bielefeldt, K. 267, 274
Bilski, J. 262
Bischoff, S. 251
Bonaz, B. 274, 275
Bonior, J. 262, 273
Booth, C.E. 267
Bradley, J.M. 249
Brammer, M.J. 254
Brinkman, B. 264
Brodacz, B. 270
Brown, D.R. 271
Browning, K.N. 272
Bruijnzeel, A.W. 277
Brzozowski, T. 261, 270
Bueno, L. 274, 275
Burr, R.L. 276
Burton, D. 264

Cain, K.C. 276
Calatayud, S. 271
Camilleri, M. 264
Cenac, N. 275
Chang, L. 276
Chen, C.-Y. 261
Christen, M.O. 275
Christensen, J. 258
Christofi, F. 250
Cieszkowski, M. 262
Clare, S. 254
Coelho, A.M. 275
Coen, S. 254
Cooke, H.J. 250
Corneliussen, B. 252
Coutinho, S.V. 253, 260
Csáti, S. 271

Danzer, M. 269
De Giorgio, R. 250
Dembinski, A. 262
Dexing Zang 277
Dockray, G.J. 264
Drozdowicz, D. 270
D'Alessio, D.A. 252

Ekkelenkamp, K. 277
El-Haj, T. 273
Eliakim, R. 272
Enck, P. 256, 265, 278
Ennes, H.S. 252
Esplugues, J.V. 271

Falzone, M.R. 273
Fan, Q. 272
Farthing, M.J.G. 273
Feuerstein, C. 274
Fioramonti, J. 274, 275
Fournet, J. 274, 275
Fowler, C.J. 256
Frankhuijzen, A.C. 277
Fujita, M. 266

Garcia-Villar, R. 275
García-Zaragozá, E. 271
Gaykema, R.P.A. 272
Gebhart, G.F. 267, 274, 275
Gietzen, D.W. 252
Glass, M.E. 266
Glatzle, J. 258, 268
Goehler, L.E. 255, 272
Green, B.T. 271
Gregory, J. 254
Grodd, W. 278
Grundy, D. 251, 267
Guerrini, S. 258
Gui, A. 265
Guiard, B. 275

Hahn, E.C. 270
Hahn, E.G. 261
Hall, G. 265
Hamdy, S. 257
Hanna, R. 250
Hausmann, S. 265
Heitkemper, M.M. 276
Hermann, G.H. 272
Hicks, G.A. 267
Hinninghofen, H. 278
Hobson, A. 254
Holzer, P. 259, 268, 269
Holzer-Petsche, U. 270
Hornby, P.J. 259, 269

Horváth, Gy. 270
Humphrey, P.P.A. 267

Izbéki, F. 271

Jarrett, M.E. 276
Javed, N. 250
Jaworek, J. 262, 273
Jehle, E.C. 278
Jinton, L. 252
Jocic, M. 268

Kalogeris, T.J. 268
Kamp, E.H. 275
Kapur, R.P. 249
Kim, M. 250
Kinnamon, S.C. 253
Kirkup, A.J. 251, 267
Klose, J. 265
Klosterhalfen, S. 265
Konturek, P.C. 261, 270
Konturek, S.J. 261, 262, 273
Kot, M. 262, 273
Kroese, A.B.A. 249
Kuo, B. 264
Kuratani, K. 261
Kwiecien, S. 261, 270

La Roche, B. 275
Lachey, J.L. 252
Lal, S. 264
Lamy, C. 274
Leja, A. 262, 273
Leray, V. 275
Leslie, F.C. 264
Linden, D.R. 249
Lippe, I.Th. 259
Liu, J. 265
Liu, M. 263
Lonovics, J. 271, 274
Lotze, M. 278
Lukovetski, E. 265
Lyte, M. 271, 272

Majka, J. 262
Mandal, B.K. 264
Martínez, V. 263
Martínez-Cuesta, M.A. 271
Marvízón, J.C. 252, 253
Mawe, G.M. 249
Mayer, E.A. 252, 253, 276
Mckinzie, S. 264
McLaughlin, J. 251
McLaughlin, J.T. 264
McRoberts, J.A. 252, 253

Miampamba, M. 259
Michel, K. 251
Michl, T. 268
Miftakhov, R.N. 258
Miller, J.C. 253
Million, M. 259, 260, 261
Miselis, R.R. 278
Mittal, R.K. 265
Moerschbaecher, J.M. 269
Moran, T.H. 263
Mulè, F. 273
Naliboff, B.D. 276

Narain, C. 254
Nawrot, K. 262, 273
Ng, V.W.K. 254
Nimaggadda, K. 272

Orr, W.C. 276
Otto, B. 265
Owyang, C. 260
Ozaki, N. 267, 274
O'Connor, M. 264

Pachnis, V. 249
Painsipp, E. 269
Pajdo, R. 261, 270
Partosoedarso, E.R. 251, 259
Pawlik, M. 261, 270
Pawlik, W. 262, 273
Perscha-Lippe, I.Th. 269
Peters, S. 251
Ploghaus, A. 254
Poole, S. 273
Ptak, A. 270

Quintana, E. 271

Rawlins, J.N.P. 254
Raybould, H.E. 258, 268
Reeve Jr, J.R. 258
Rivier, J. 260, 263
Robin, C. 258
Robinson, D.R. 267
Rogers, R.C. 272
Ron, Y. 265
Rosztóczy, A. 274
Rouzade-Dominguez, M.L. 278

Sablad, M.R. 253
Sanders, K.M. 250
Santos, J. 255
Saunders, P. 260
Schemann, M. 251

Schey, R. 265

Schicho, R. 259, 269

Schuligoi, R. 268

Seeley, R. J. 252

Sendur, R. 262, 273

Sharkey, K.A. 249

Sinniger, V. 274, 275

Slice, L.W. 258

Smedh, U. 263

Song, B. 252

Stachura, J. 273

Stam, R. 277

Sternini, C. 258

Stockhorst, U. 265

Szlachcic, A. 262

Taché, Y. 259, 260, 261, 263

Tada, H. 266

Takahashi, T. 266

Takeda, T. 265

Thomforde, G. 264

Thompson, D.G. 254, 264

Thompson, J. 276

Tjwa, E.T.T.L. 249

Tomaszewska, R. 273

Tracey, I. 254

Travagli, R.A. 272

Tso, P. 253, 263, 268

Vale, W. 260

Valentino, R. J. 278

Vanner, S. 255

Varro, A. 264

Vergnolle, N. 256, 275

Viramontes, B. 264

Wang, L. 260, 263

Wang, X. 252

Wang, Y.H. 268

Ward, S.M. 250

Wardi, J. 265

Warzecha, Z. 262

Wei, J.Y. 268

Wiegant, V.M. 277

Wietek, B. 278

Wiley, J.W. 256

Williams, S.C.R. 254

Wise, R. 254

Wittmann, T. 271, 274

Wong, H. 258

Wright, B. 272

Wu, H. 249

Wu, X.Y. 260

Yaguez, L. 254

Ying Li 260

Zhang, J. 272

Zhao, X. 278

Zhao, Y. 260

Zhiqiang Wang 277

Zhixiang Shen 277

Zittel, T.T. 258, 268