L-Ilf3 and L-NF90 traffic to the nucleolus granular component: alternatively-spliced exon 3 encodes a nucleolar localization motif.

Wildriss Viranaicken, Laila Gasmi, Alexandre Chaumet, Christiane Durieux,
Virginie Georget, Philippe Denoulet, Jean-Christophe Larcher

<figure><img src="image_1.png" /></figure>

To cite this version:

Wildriss Viranaicken, Laila Gasmi, Alexandre Chaumet, Christiane Durieux, Virginie Georget, et al. L-Ilf3 and L-NF90 traffic to the nucleolus granular component: alternatively-spliced exon 3 encodes a nucleolar localization motif.. PLoS ONE, 2011, 6 (7), pp.e22296. 10.1371/journal.pone.0022296. hal-00627722

HAL Id: hal-00627722

https://hal.science/hal-00627722v1

Submitted on 13 Jun 2018

**HAL** is a multi-disciplinary open access
archive for the deposit and dissemination of sci-
entific research documents, whether they are pub-
lished or not. The documents may come from
teaching and research institutions in France or
abroad, or from public or private research centers.

L'archive ouverte pluridisciplinaire HAL, est
destinée au dépôt et à la diffusion de documents
scientifiques de niveau recherche, publiés ou non,
émanant des établissements d'enseignement et de
recherche français ou étrangers, des laboratoires
publics ou privés.

<figure><img src="image_2.png" /></figure>
# L-Ilf3 and L-NF90 Traffic to the Nucleolus Granular Component: Alternatively-Spliced Exon 3 Encodes a Nucleolar Localization Motif

Wildriss Viranaicken<sup>1,2¤a</sup>, Laila Gasmi<sup>1,2¤b</sup>, Alexandre Chaumet<sup>1,2¤c</sup>, Christiane Durieux<sup>3¤d</sup>, Virginie Georget<sup>4¤e</sup>, Philippe Denoulet<sup>1,2</sup>, Jean-Christophe Larcher<sup>1,2*</sup>

1 UPMC Univ Paris 06, UMR 7622, Laboratoire de Biologie du Développement, Paris, France, 2 CNRS, UMR 7622, Laboratoire de Biologie du Développement, Paris, France, 3 Institut Jacques Monod, UMR7592 CNRS - Université Denis Diderot, Paris, France, 4 UPMC Université Paris 06, IFR 83, Institut de Biologie Intégrative, Paris, France

## Abstract

Ilf3 and NF90, two proteins containing double-stranded RNA-binding domains, are generated by alternative splicing and involved in several functions. Their heterogeneity results from posttranscriptional and posttranslational modifications. Alternative splicing of exon 3, coding for a 13 aa N-terminal motif, generates for each protein a long and short isoforms. Subcellular fractionation and localization of recombinant proteins showed that this motif acts as a nucleolar localization signal. Deletion and substitution mutants identified four arginines, essential for nucleolar targeting, and three histidines to stabilize the proteins within the nucleolus. The short isoforms are never found in the nucleoli, whereas the long isoforms are present in the nucleoplasm and the nucleoli. For Ilf3, only the posttranslationally-unmodified long isoform is nucleolar, suggesting that this nucleolar targeting is abrogated by posttranslational modifications. Confocal microscopy and FRAP experiments have shown that the long Ilf3 isoform localizes to the granular component of the nucleolus, and that L-Ilf3 and L-NF90 exchange rapidly between nucleoli. The presence of this 13 aminoacid motif, combined with posttranslational modifications, is responsible for the differences in Ilf3 and NF90 isoforms subcellular localizations. The protein polymorphism of Ilf3/NF90 and the various subcellular localizations of their isoforms may partially explain the various functions previously reported for these proteins.

**Citation:** Viranaicken W, Gasmi L, Chaumet A, Durieux C, Georget V, et al. (2011) L-Ilf3 and L-NF90 Traffic to the Nucleolus Granular Component: Alternatively-Spliced Exon 3 Encodes a Nucleolar Localization Motif. PLoS ONE 6(7): e22296. doi:10.1371/journal.pone.0022296

**Editor:** Axel Imhof, Ludwig-Maximilians-Universität München, Germany

**Received** January 25, 2011; **Accepted** June 23, 2011; **Published** July 19, 2011

**Copyright:** © 2011 Viranaicken et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

**Funding:** This work was supported by the Centre National de la Recherche Scientifique and the Ministère de l'Enseignement supérieur et de la Recherche. J.-C. L. and C. D. were supported by the Ministère de l'Enseignement supérieur et de la Recherche (ACI BCMS 2004, decision n°04 5 495) and the "Fédération pour la Recherche sur le Cerveau". W. V. and A. C. hold fellowships from the Ministère de l'Enseignement supérieur et de la Recherche. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

**Competing Interests:** The authors have declared that no competing interests exist.

* E-mail: jclarche@snv.jussieu.fr

¤a Current address: GRI-CYROI Université de la Réunion, Sainte Clotilde, France

¤b Current address: Institut du Fer à Moulin UMRS 839, Paris, France

¤c Current address: Institute of Molecular and Cell Biology, Singapore

¤d Current address: INSERM, Paris, France

¤e Current address: CNRS-CRBM UMR 5237, Montpellier, France

## Introduction

Compartmentalization of proteins is a key mechanism for regulating many cellular processes and/or restricting site activity of proteins. To find their right place in cells, proteins are generally endowed with signals that target them to the appropriate subcellular compartment. This destination can represent either the final working place of proteins, a transient localization or a means for certain other proteins to be sequestered. These signals are recognized and processed by specialized cell machineries. Another way to localize proteins is to first direct their mRNA prior to their translation, a mechanism requiring specific nucleotide signals as well as escort proteins to process these signals.

In this context, we previously characterized two proteins, Interleukin enhancer binding factor 3 (Ilf3) and Nuclear Factor 90 (NF90), that interact with the axonal targeting element of Tau mRNA and move with it from the nucleus to the axon hillock [1].

Belonging to the family of proteins containing double-stranded RNA-binding domain(s) (dsRBM; [2]), these two proteins are generated by alternative splicing from a single gene [3,4]. This event provides two proteins with common N-terminal and central domains and a specific C-terminal domain [3–5].

An heterogeneity of Ilf3 and NF90 was evidenced after separation by 2-D PAGE [1] with at least 12 and 8 spots, respectively. This polymorphism is partially due to an alternative splicing of exon 3 in the 5' region of their premessenger RNA, which generates long (L) and short (S) isoforms for Ilf3 and NF90 [5]. These isoforms differ by the presence or the absence of a specific basic N-terminal sequence of 13 residues (ALYHHH-FITRRRR) localized just downstream the initiation methionine. The polymorphism of Ilf3 and NF90 arising from alternative splicing events is also complexified by at least two posttranslational modifications: arginine-methylation by protein-arginine methyl-transferase I in the RGG motif [6] and phosphorylation by PKR

<figure><img src="image_3.png" /></figure>
[4,7,8], the DNA protein kinase [9] or the AKT kinase in T-cells [10].

The existence of several Ilf3 and NF90 isoforms [1,3,5] may reflect their numerous described functions: transcriptional activation [11–14], eukaryotic and viral RNA binding [1,15–20], translational inhibition [21–24] or enzymatic regulation [6,7]. This polymorphism could also explain the various subcellular localizations described [1,25,26]. Since Ilf3 and NF90 are recovered in both nuclear and cytoplasmic fractions [1], they may shuttle between these compartments [27]. Finally, the interaction of Ilf3 and NF90 with protein and/or RNA partners may be regulated by posttranscriptional and/or posttranslational modifications [13,23].

Following our original report of an additional splicing event that generates multiple Ilf3 and NF90 protein profiles isoforms [5], we herein investigated the potential influence of Ilf3 and NF90 posttranscriptional and posttranslational heterogeneities on their subcellular localization. The N-terminal location of the alternative 13-aa segment enriched in basic residues led us to search for a specific role to this signal-type motif. Accordingly, we fused the N-terminal sequence of Ilf3/NF90, containing or not the 13 residues, to GFP or a well-known cytoplasmic protein to follow whether the chimeric proteins were targeted to a specific cellular compartment. Since some chimeric proteins were targeted in the nucleolus, we performed subcellular fractionation to confirm the nucleolar localization of endogenous proteins. In addition, using deletion and substitution mutants, we investigated on the role played by different residues present in this motif. Finally, confocal microscopy and FRAP experiments were performed to study the accurate subnucleolar localization and the dynamics of the proteins.

## Results

### 1. Long Ilf3/NF90 N-terminus fused with GFP accumulates in nuclear foci

Whereas L- and S-Ilf3/NF90 isoforms exhibit the same ability to bind Tau mRNA [1], we tested whether the L-isofoms containing the specific N-terminal sequence exhibited a particular localization. Plasmids containing the GFP sequence fused in frame behind the sequences coding for the 54 first aa of the L-Ilf3/NF90 isoforms (Long-GFP) or the 41 aa of the S-Ilf3/NF90 isoforms (Short-GFP) were transfected into HeLa cells. The intracellular distribution of GFP was observed by epifluorescence microscopy.

In control cells expressing GFP alone as in cells transfected with the Short-GFP plasmids, fluorescence was observed in the cytoplasm and especially in the nucleus, where GFP has been previously shown to accumulate (Figure 1, left and middle panels). In contrast, fluorescence was barely detectable in the cytoplasm of cells transfected with the Long-GFP plasmids and rather accumulated in discrete nuclear foci that may correspond to nucleoli (Figure 1, right panels). This 13-aa sequence could thus function as an efficient nucleolar localization signal (NoLS).

### 2. Endogenous long Ilf3/NF90 isoforms are targeted to the nucleolus

To confirm the role of the 13-aa motif in nucleolar targeting and to avoid any bias associated with overexpression, the presence of endogenous L- and S-Ilf3/NF90 isoforms was examined in subcellular fractions prepared from P19 cells. Identical results were obtained with HeLa cells (data not shown). Western blotting of different fractions using a polyclonal antibody raised against common regions of the two proteins [1] revealed that the L- and S-Ilf3 isoforms were present in both cytoplasmic and nuclear fractions (Figure 2). In contrast, only the L-NF90 isoforms were

found in the nuclear fraction whereas all the NF90 isoforms were recovered in the cytoplasm. Furthermore, when purified nuclei were fractionated into nucleoplasmic and nucleolar fractions, only the L-Ilf3 and L-NF90 isoforms were found associated with the nucleoli. The identity of the different fractions was checked by immunodetecting UBF, as a nucleolar marker, and α-tubulin, as a cytosolic marker. These results strengthen the idea that the N-terminal 13-aa sequence present in the L-Ilf3 and L-NF90 isoforms acts as a potent NoLS.

To confirm the targeting of L-isofoms to the nucleolus and explore the role of their posttranslational modifications in this compartimentalization, subcellular fractions were analyzed by 2D-PAGE. For isoforms present in both cytosolic and nuclear fractions (L- and S-Ilf3 and L-NF90), the same 2-D patterns were obtained (data not shown), suggesting that the posttranslational modifications do not regulate their nucleocytoplasmic distribution. However, the comparison of the Ilf3 and NF90 heterogeneities in subnuclear compartments showed an exclusive distribution of the isoforms between the nucleolus and the nucleoplasm (Figure 3). As shown above (Figure 2), the nuclear NF90 isoforms were almost solely recovered in the nucleolar fraction (Figure 3, lower right panel). The faint signal observed in the nucleoplasmic fraction (Figure 3, middle right panel) was detected with a ten-fold longer exposure time than that obtained with the nucleolar extract and is probably due to L-NF90 isoforms trafficking between the nucleolus and the cytosol. Concerning Ilf3, only the most alkaline L-isofoms were exclusively recovered in the nucleolar fraction whereas all the other L- and S-isofoms were found in the nucleoplasm. These results confirm that the nucleolar fraction contains only isoforms of Ilf3/NF90 containing the 13 aminoacid motif and strongly suggest that posttranslational modifications of L-Ilf3 isoforms, evidenced by their different more acidic pI, negatively regulate their nucleolar localization.

### 3. A cytoplasmic protein fused to Long Ilf3/NF90 N-terminus is targeted to the nucleolus

Since GFP can enter the nucleus, we tested the ability of the 13-aa motif to target to the nucleus and nucleolus a well-known cytoplasmic protein, the human eukaryotic releasing factor 1 (heRF1). The heRF1 coding sequence was fused at the 3' end of the sequence coding for the first 54 aa of the L-Ilf3/NF90 isoforms (NoLS-heRF1) or for the first 41 aa of the S-Ilf3/NF90 isoforms (N-heRF1). The constructs were transfected into HeLa cells and the heRF1 localization was observed by immunofluorescence microscopy (Figure 4).

In control cells transfected with N-heRF1 (Figure 4, middle panels), anti-eRF1 antibodies stained only the cytoplasmic compartment, as expected for endogenous heRF1 (Figure 4, upper panels). By contrast, in cells expressing NoLS-heRF1 (Figure 4, lower panels), nuclei were also decorated (Figure 4, merge panels). Furthermore, fluorescence was also associated with nuclear structures corresponding to nucleoli (Figure 4, arrows in lower panels). In conclusion, the 13-aa motif appears to be necessary and sufficient to import a cytoplasmic protein into the nucleus and to further drive it to the nucleolus. This motif, only present in the L-Ilf3 and L-NF90 isoforms, thus encompasses a dual NLS and NoLS.

### 4. Aminoacid deletion or substitution in the NoLS modulate the subnuclear distribution

To identify key residues for nucleolar targeting in the 13-aa sequence, deletion and substitution mutants were constructed using NoLS-GFP sequence (GFP coding sequence fused at the 3'

<figure><img src="image_4.png" /></figure>
<figure><img src="image_5.png" /><figcaption>**Figure 1.** Subcellular localization of GFP fused with the short or long N-terminal sequence of Ilf3/NF90. Plasmids pEGFP-N1 (Control, left panels), pEGFP-N1-Ilf3/NF90 common N-terminal short sequence (Short-GFP, mid panels) and pEGFP-N1-Ilf3/NF90 common N-terminal long sequence (Long-GFP, right panels) were transfected into HeLa cells. After 24 hours, cells were fixed and co-stained with anti-α-tubulin antibody (α-Tub) and DAPI. GFP or GFP fusion proteins appear in green, α-tubulin in red and DAPI staining in blue. Arrows point to intranuclear foci.
doi:10.1371/journal.pone.0022296.g001</figcaption></figure>

end of the sequence coding for the first 54 aa of the L-Ilf3/NF90 isoforms). Expression vectors were transfected into HeLa cells and after 24 hours cells were processed for indirect immunofluorescence with anti-B23 monoclonal antibodies. The intranuclear distribution of GFP and B23 was then observed by confocal microscopy (Figure 5). The three upper rows of figure 5A show the subcellular localization of GFP alone, wild-type NoLS-GFP and N-GFP (GFP coding sequence fused at the 3' end of the sequence coding for the first 41 aa of the S-Ilf3/NF90 isoforms). While NoLS-GFP (positive control) accumulated in nucleoli, GFP alone and N-GFP (negative controls) uniformly labeled the whole nucleus and were seemingly excluded from nucleoli.

The 13-aa sequence contains a four-arginine stretch, which fits well with eukaryotic or viral NoLS consensus, K/R-K/R-X-K/R [28–30]. Deletion of the four arginines (Figure 5A, Δ4R-GFP row) led to the loss of nucleolar localization, as indicated by the absence of colocalization with B23.

As shown above, the 13-aa sequence can drive heRF1 into the nucleus (Figure 4). Given the similarity between the NoLS and basic NLS, one can ask why any NLS-containing protein is not automatically targeted to the nucleolus. What is required in addition to these four positively-charged residues to become an

efficient and specific NoLS? Looking at the residues adjacent to the four-arginine stretch within the 13-aa sequence, two features could be singled out: a threonine residue predicted to be phosphorylated by protein kinase C and a three-histidine stretch.

When the threonine was replaced by alanine (T10A) or by the phosphomimetic aspartate (T10D), no difference with the positive control (Figure 5A, NoLS-GFP) was observed (Figure 5A, T10A-GFP and T10D-GFP rows), indicating that neither this threonine residue nor its putative phosphorylation are involved in NoLS function. When the three histidines were deleted (Figure 5B, Δ3H-GFP row) or replaced by three alanines (Figure 5B, 3H->3A-GFP row) or three phenylalanine (Figure 5B, 3H->3F-GFP row), an intermediate situation between the positive and negative controls was observed: nucleoli were less fluorescent and the nucleoplasm appeared more fluorescent than in the positive NoLS-GFP control. Fluorescence quantification indicated that, in the Δ3H-GFP, 3H->3A-GFP and 3H->3F-GFP experiments, nucleoli emitted only two-thirds of the GFP fluorescence recovered in the positive control (cells quantified: 39, 14, 21 and 32, respectively). These results indicate that the loss or the replacement of the three histidines by three alanines or phenylalanines significantly weakens the nucleolar targeting/retention.

<figure><img src="image_6.png" /></figure>
<figure><img src="image_7.png" /><figcaption>**Figure 2.** Subcellular distribution of Ilf3 and NF90 in P19 cells. After subcellular fractionation, proteins from identical percentages of each fraction were submitted to SDS-PAGE, blotted onto nitrocellulose and immunodetected with the serum Ab78 raised against Ilf3 and NF90 (S.E.: short exposure time; L.E.: long exposure time), anti-UBF serum (UBF) or anti-α-tubulin antibody (α-tub.). Molecular weight markers (kDa) are indicated at the right.
doi:10.1371/journal.pone.0022296.g002</figcaption></figure>

To assess the role of positive charges carried by the three histidine residues, they were substituted by three lysines or glutamates (Figure 5B, 3H->3K-GFP and 3H->3E-GFP rows).

<figure><img src="image_8.png" /><figcaption>**Figure 3.** Ilf3 and NF90 polymorphism in nuclear fractions purified from P19 cells. Ilf3 and NF90 from P19 cell nuclear fractions were submitted to 2-D PAGE and immunodetected with polyclonal antibody 78. Arrows positioned in the same coordinates in Ilf3 or NF90 panels indicate the positions of Ilf3 and NF90 long and short isoforms. The faint signal in the middle right panel was detected with a ten fold longer time exposure than that of the other panels.
doi:10.1371/journal.pone.0022296.g003</figcaption></figure>

With the positively-charged lysines, the fluorescence levels of nucleoli were similar to those of NoLS-GFP (96%, n = 20) whereas with the negatively-charged glutamates (n = 11), the nucleoli fluorescence levels were similar or weaker to those of Δ3H-GFP, 3H->3A-GFP and 3H->3F-GFP mutants. Finally, when a double 3H->3K/Δ4R-GFP mutant was tested, no nucleolar targeting was observed (Figure 5B, 3H->3K/Δ4R-GFP row) similarly to Δ4R-GFP mutant. Altogether, these results show that the three added lysines cannot substitute for the four missing arginines and strongly suggest that the positive charges of the three histidines may be important to retain the proteins in the nucleolus. The four arginines should then be required to drive a cytoplasmic protein into the nucleus and, from there, up to the nucleolus whereas the three histidines should increase its retention time in the nucleolus, maybe through specific interactions with nucleolar constituents.

## 5. Long Ilf3/NF90 isoforms are present in the granular component of the nucleolus

To better define the subnucleolar localization of long isoforms, the GFP sequence was fused in frame after the S-Ilf3, L-Ilf3 or L-NF90 sequences. Constructs were transfected into HeLa cells and after 24 hours cells were processed for indirect double immunofluorescence with human anti-fibrillarin serum and anti-B23 monoclonal antibodies. The intranuclear distribution of GFP and markers was then observed by confocal microscopy (Figure 6).

In control cells expressing GFP alone as in cells transfected with the S-Ilf3 constructs, GFP fluorescence was observed in the whole nucleoplasm without any particular localization (Figure 6, Control and S-Ilf3 rows, left panels), whereas in cells transfected with the L-Ilf3 or L-NF90 constructs, GFP fluorescence was mostly restricted to the nucleoli (Figure 6, L-Ilf3 and L-NF90 rows, left panels). The fibrillarin and B23 antibodies decorating dense fibrillar and granular components, respectively (Figure 6, vertical middle panels) and the merged images showing a strict

<figure><img src="image_9.png" /></figure>
<figure><img src="image_10.png" /><figcaption>**Figure 4.** Subcellular localization of human eRF1 fused with short or long N-terminal sequence of Ilf3/NF90. Plasmids pCMV-heRF1-Ilf3/NF90 short N-terminal sequence (N-heRF1, mid panels) and pCMV-heRF1-Ilf3/NF90 long N-terminal sequence (NoLS-heRF1, lower panels) were transfected into HeLa cells. After 24 hours, untransfected (Control, upper panels) or transfected cells were co-stained with anti-heRF1 antibody (heRF1), anti-α-tubulin antibody (α-Tub) and DAPI. Endogenous heRF1 or heRF1 recombinant fusion proteins appear in green, α-tubulin in red and DAPI in blue. Arrows point to intranuclear foci corresponding to nucleoli.
doi:10.1371/journal.pone.0022296.g004</figcaption></figure>

colocalization of L-Ilf3 and L-NF90 isoforms with B23 and not with fibrillarin (Figure 6, L-Ilf3 and L-NF90 rows, right panels), we concluded that the unmodified L-Ilf3 and L-NF90 isoforms appear to be mainly present in the nucleolus granular component [31].

## 6. Dynamic behavior of Ilf3 and NF90 during nucleolar disruption and reformation

DRB, an inhibitor of protein casein kinase II that phosphorylates nucleolar proteins, such as nucleophosmin, nucleolin and UBF, has been described to disorganize the nucleolus [32]. To analyze the behavior of Ilf3/NF90 during the reversible disruption and reformation of nucleoli, we have compared their dynamics with that of B23 during these processes.

When L-NF90-GFP and mcherry-B23 were co-expressed in HeLa cells, both proteins colocalized in the granular component (Figure 7, upper panels), as L-Ilf3 (Figure 5; lower panels). After treatment with DRB, the mcherry fluorescence was greatly reduced whereas the L-NF90-GFP fluorescence diffused in the nucleoplasm (Figure 7, middle panels). One hour after DRB removal, a partial restoration of nucleolar structures was observed. While B23 seemed to rapidly and almost totally localize into the reforming nucleoli, only a part of L-NF90 also relocalized with

B23. The remaining L-NF90 was detected at the periphery of reforming nucleoli, around the B23 foci (Figure 7, lower panels). Identical results were obtained with L-Ilf3-GFP (data not shown).

## 7. L-Ilf3 and L-NF90 are highly dynamic and exchange between nucleoli

Knowing that L-Ilf3 and L-NF90 can dissociate from and reassociate with nucleoli (Figure 7), FRAP experiments using L-Ilf3-GFP, L-NF90-GFP and B23-GFP were performed to analyze precisely their kinetics. In HeLa cell nuclei, one nucleolus was photobleached then GFP fluorescence recovery was followed during 240 seconds, using one of the other non-bleached nucleoli of the same nucleus and one nucleolus of a neighboring cell as controls (Figure 8A).

Figure 8B shows GFP fluorescence recordings from the bleached nucleolus (green curve), from one adjacent nucleolus in the same nucleus (purple curve) and from one nucleolus of a distinct cell (yellow curve). The bleached nucleolus was rapidly recolonized by L-NF90-GFP and its fluorescence level reached a plateau after 90 seconds post-bleach. At the same time, we observed that the fluorescence level of the adjacent nucleolus slightly and progressively decreased after the bleach and was

<figure><img src="image_11.png" /></figure>
<figure><img src="image_12.png" /><figcaption>**Figure 5.** Subnuclear distribution of GFP fused to deletion or substitution mutants of Ilf3/NF90 NoLS. (A) Plasmids pEGFP-N1 (Control), pEGFP-N1-Ilf3/NF90 common N-terminal long region (NoLS-GFP), pEGFP-N1-Ilf3/NF90 common N-terminal short region (N-GFP), pEGP-N1/deletion mutant of the four-arginine stretch from the NoLS (Δ4R-GFP), pEGP-N1/substitution mutant of the threonine 10 by alanine (T10A-GFP) and pEGP-N1/ substitution mutant of the threonine 10 by aspartate (T10D-GFP) and (B) plasmids pEGP-N1/deletion mutant of the three-histidine stretch (Δ3H-GFP), pEGP-N1/substitution mutant of the three-histidine stretch by three alanines (3H→3A-GFP), three phenylalanines (3H→3F-GFP), three lysines (3H- >3K-GFP) or three glutamates (3H→3E-GFP) and pEGP-N1/substitution mutant of the three-histidine stretch by three lysines/deletion mutant of the four-arginine stretch (3H→3K/Δ4R-GFP) were transfected into HeLa cells. After 24 hours, cells were co-stained with the monoclonal anti-B23 antibody (B23) and DAPI. After confocal microscopy acquisition, focal planes exhibiting a B23 optimal signal were chosen. GFP fusion proteins appear in green, B23 in red and DAPI in blue.
doi:10.1371/journal.pone.0022296.g005</figcaption></figure>

stabilized after about 60 seconds. During this experiment, the
fluorescence level of the nucleolus of the neighbor cell remained
constant. These results indicate that L-NF90 behaves as a highly-
dynamic protein which is rapidly exchanged between the different
nucleoli within the same nucleus. Experiments performed with L-
Ilf3-GFP and B23-GFP revealed the same dynamic exchange
between nucleoli (data not shown), as reported also for several
nucleolar proteins (38).

Computation of fluorescence recovery recordings from inde-
pendent experiments with L-Ilf3-GFP (n = 14), L-NF90-GFP
(n = 15) and B23-GFP (n = 11) is shown in figure 8C. t¹/²
recovery, deduced for each protein (L-Ilf3: 38.9 seconds; L-
NF90: 19.8 seconds; B23: 4 seconds and 39 seconds), indicated
that L-Ilf3 and L-NF90 diffuse in a monodimensional manner
whereas B23 appears to be composed of two mobile subpopula-
tions, one diffusing more rapidly than the other, that is in
opposition with previous report [33]. Finally, data analysis showed
that L-Ilf3 mobile fraction represents 70% of total proteins, 93%
for L-NF90 and 85% for B23.

Altogether, FRAP experiments indicate that L-Ilf3 and L-NF90
are each composed by a single population of highly mobile
proteins that can exchange rapidly from one nucleolus to another
within the same nucleus.

**Discussion**

Previous studies showed that Ilf3 and NF90 are produced by
alternative splicing in the 3' region of their premessenger RNA

[3,4]. In addition, alternative splicing of exon 3 leads to the
presence or absence of a 13-aa peptide at the N-terminus of both
proteins [5]. Herein, we investigated in HeLa cells the influence of
this sequence on the subcellular localization of recombinant fusion
proteins (Figures 1, 4). We also analyzed the colocalization of
GFP-tagged Ilf3/NF90 and B23 in HeLa cells and the distribution
of endogenous Ilf3/NF90 in subcellular fractions of HeLa and P19
cells. Altogether, our results strongly suggest that the N-terminal
13 aa acts as a NoLS that also favors entry of the protein into the
nucleus.

This NoLS contains three histidines followed by four arginines, forming a stretch of basic residues. Analysis of 61 previously-characterized or putative NoLS present in well-defined nucleolar proteins showed the presence of the nucleolar localization consensus motif, K/R-K/R-X-K/R [34,35]. Many proteins are constantly or transiently associated with the nucleolus. Many of them contain a NoLS in which the consensus can be present in single or multiple copies that can be positioned anywhere in the primary sequence [28-30]. Surprisingly, computer analysis of these 61 NoLS showed that, among the eight possible combinations, the homogenous KKXK and RRXR motifs are found preferentially (30% and 40%, respectively; Table 1, bottom lane). Concerning the chemical nature of the residue X (Table 1, right column) and its observed frequency, as compared with that in vertebrate proteins, we noted a strong occurrence of proline (~9.7% vs 5%), lysine (16.1% vs 7.2%), asparagine (17.7% vs 4.4%) and arginine (22.6% vs 4.2%) at this position. Acidic aspartate and glutamate were never found at this position, nor cysteine, tyrosine and histidine.

<figure><img src="image_13.png" /></figure>
<figure><img src="image_14.png" /><figcaption>**Figure 6.** Subnuclear distribution of exogenously-expressed S-Ilf3, L-Ilf3 and L-NF90 isoforms in HeLa cells. Plasmids pEGFP-N1 (Control row), pEGFP-N1-S-Ilf3 (S-Ilf3 row), pEGFP-N1-L-Ilf3 (L-Ilf3 row) or pEGFP-N1-L-NF90 (L-NF90 row) were transfected into HeLa cells. After 24 hours, cells were co-stained with human anti-fibrillarin serum (Fibrillarin) and monoclonal anti-B23 antibody (B23). After confocal microscopy acquisition, focal planes were chosen to obtain optimal fibrillarin and B23 signals. DAPI (not shown here) was used to define the nuclear limits (white drawings). GFP and GFP fusion proteins appear in green, fibrillarin in red and B23 in blue.
doi:10.1371/journal.pone.0022296.g006</figcaption></figure>

In the case of Ilf3 and NF90 L-isof orm s, analysis of muta nts indicated, in addition to the indispensable role of the four arginines to direct proteins to the nucleolus, the presence of positive charges close to the arginine stretch seems to be very important to retain these proteins in the nucleolus. The deletion or the replacement of the three histidines by uncharged (alanine or phenylalanine) or negatively-charged (glutamate) residues effec- tively decreases the efficiency of the nucleolar targeting by one third. A *contrario*, their substitution by three lysines with their strong and permanent positive charges maintains this efficiency.

Interestingly, whereas the distribution of endogenous Ilf3 in the nucleus revealed that most of Ilf3 was recovered in the nucleoplasm (Figures 2 and 3), the overexpression of L-Ilf3 showed a preferential localization in nucleoli (Figure 6). This observation could be explained by the fact that the unmodified L-Ilf3 isoforms are only recovered in the nucleoli while the posttranslationally-modified isoforms are in the nucleoplasm (Figure 3). Indeed, if we consider that overexpressed L-Ilf3 is not posttranslationally-modified because the modification enzymes are titrated by the excess of substrate proteins, we can explain why the majority of the chimeric L-Ilf3-GFP is associated with the nucleoli (Figure 6) and not found in the nucleoplasm, as endogenous L-Ilf3 (Figures 2, 3). Nuclear fraction of endogenous L-NF90 being exclusively associated with the nucleoli (Figure 2), independently of

their posttranslational modifications status (Figure 3), the overex-
pressed L-NF90 is also only localized in the nucleoli (Figure 6).
These observations could explain why a L-Ilf3 or L-NF90
overexpression leads to a cellular death (data not shown), certainly
due to a perturbation of the nucleolar organization and functions
resulting from an overaccumulation of overexpressed proteins.

The nucleolar localization of L-Ilf3/NF90 isoforms resembles that of proteins involved in nucleocytoplasmic transport of mRNA [36] or mRNA editing [37]. For these proteins, it was suggested that they assemble with their cargo mRNA into RNP directly in the nucleolus or first in the nucleoplasm and then localize to the nucleolus [38]. In both cases, RNP particles leave the nucleolus after an eventual binding of nucleolar proteins and/or RNAs and are then exported to the cytoplasm. The functional reason for rerouting RNP particles *via* the nucleolus on the way to the cytoplasm is not clear, but two hypotheses can be considered. Either the passage by the nucleolus is a checkpoint to ensure the functional integrity of RNP complexes, or it represents a sequestration step allowing some negative regulation to occur on mRNA translation and/or protein activity [38]. Whatever it may be, since Ilf3/NF90 have been previously reported to interact with several RNAs [1,15-20], the idea of a transient association of RNP containing Ilf3/NF90 with the nucleolus was reinforced by data showing that they interact with the nucleolar protein ADAR [39].

<figure><img src="image_15.png" /></figure>
<figure><img src="image_16.png" /><figcaption>**Figure 7.** Subcellular distribution of exogenously-expressed L-NF90 isoforms in HeLa cells treated with DRB. Plasmids pEGFP-N1-L-NF90 (L-NF90-GFP) and mcherry-B23 (B23-mcherry) were transfected into HeLa cells. 24 hours later, cells were fixed either immediately (Control, upper panels) or after a DRB treatment during two hours (DRB 2 h, mid panels) followed by a chase of one hour (Chase 1 h, lower panels). GFP and mcherry fusion proteins appear in green and red, respectively. doi:10.1371/journal.pone.0022296.g007</figcaption></figure>

At the end of mitosis or after a DRB treatment, the timing of incoming proteins in the reassembling nucleoli depends on their subnucleolar localization, hence on their function [31,32,40]. Indeed, the proteins recovered in the fibrillar component and implied in early rRNA processing steps, e.g., fibrillarin and UBF, arrived at first whereas the proteins of the granular component responsible for late rRNA processing stages, e.g., B23 and Nop52, relocalized to the nucleolus later [31,32,40]. Even if some L-NF90 isoforms can reintegrate rapidly the granular component, the relocalization of most of them occurs much later. This difference in the timing of the relocalization of L-NF90 and the other granular component proteins would suggest that L-NF90 (and certainly unmodified L-Ilf3 also present there) could be implicated either in the very last stages of rRNA processing and/or preribosomes assembly or in other yet unknown functions.

FRAP experiments have shown that L-Ilf3 and L-NF90 exhibit a highly dynamic behavior and can exchange between different nucleoli within the same nucleus. This corresponds to a typical behavior for numerous nucleolar proteins [31]. These internucleolar exchanges indicate that all the nucleoli of a given nucleus can be considered as a single nuclear compartment instead of independent components. In the FRAP experiments described in this paper, only the fluorescence recovery of L-Ilf3-GFP and L-NF90-GFP present in nucleoli is measured, assuming that the endogenous proteins behave similarly. The t¹/₂ recovery values corresponding to nucleoplasmic or cytoplasmic Ilf3/NF90 may be different from those reported here.

Herein, we showed that the N-terminal 13-aa sequence of the L-Ilf3 and L-NF90 proteins, encoded by the alternatively-spliced exon 3, allows their subcellular localization to nucleoli, thus acting as a NoLS. Moreover, this particular sequence is necessary and

sufficient to target unrelated cytoplasmic polypeptides to the nucleolus.

Considering the numerous cellular functions previously reported for Ilf3 and NF90 [1,6,7,11-24] and their multiple cellular localizations ([1,23,26,27]; our results), and given that all isoforms are not present in all cellular compartments, each isoform may have specialized functions. Moreover, taking into consideration the important polymorphism of Ilf3/NF90, it is obvious that this protein heterogeneity is not generated solely by alternative splicing events, but also by posttranslational modifications, such as arginine methylation [6] and phosphorylation [4,7,8]. Analyses of their implication in the subcellular localization of Ilf3/NF90 and/or in the regulation of their functions are currently in progress.

## Materials and Methods

### Cell culture

HeLa and P19 cells were cultured as described [5]. When mentioned, cultured cells were treated for two hours by addition of 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole (DRB; Sigma-Aldrich, St Louis, MO, USA) at the concentration of 240 µmole.L⁻¹.

### Indirect immunofluorescence

HeLa cells were fixed as described [1]. Subsequent steps were performed in PBS supplemented with 0.2% (v/v) Triton X100 and 1% (w/v) bovine serum albumine. Cells were stained for one hour at room temperature with monoclonal anti-α-tubulin DM1A (1:1000; Sigma-Aldrich), polyclonal anti-heRF1 antibodies (1:200), human polyclonal anti-fibrillarin D157 serum (1:500) and/or monoclonal anti-B23 antibody FC82291 (1:500; Sigma-Aldrich). Primary antibodies were revealed using the Alexa Fluor 488 goat

<figure><img src="image_17.png" /></figure>
<figure><img src="image_18.png" /></figure>

<figure><img src="image_19.png" /></figure>

<figure><img src="image_20.png" /></figure>

**Figure 8.** Fluorescence recovery in HeLa cell nucleoli after photobleaching (FRAP) of GFP-tagged L-Ilf3, L-NF90 or B23. Plasmids pEGFP-N1-L-Ilf3, pEGFP-N1-L-NF90 or pEGFP-N1-B23 were transfected into HeLa cells. After 24 hours, living cells were subjected to photobleaching. A. In HeLa cells expressing L-NF90-GFP, one nucleolus was targeted for laser bleaching (left panel, green circle in the upper cell) whereas another nucleolus from the same cell (blue circle in the upper cell) and a nucleolus from a distinct cell (yellow circle in the lower cell) were marked to serve as controls. Images were acquired every two seconds during 40 seconds before, immediately after or during 240 seconds after bleaching (prebleach, bleach, post-bleach 6 and 240 seconds, respectively). B. Fluorescence recordings emitted from the 3 delimited regions in A (green: bleached nucleolus; purple: control nucleolus from the same cell; yellow: control nucleolus from another unbleached cell). C. Kinetics of mean fluorescence recovery after photobleaching of GFP-tagged L-Ilf3 (n = 14), L-NF90 (n = 15) or B23 (n = 11) after a normalization of fluorescence intensity. The t¹/₂ of mean fluorescence recovery are indicated in the figure.
doi:10.1371/journal.pone.0022296.g008

<figure><img src="image_21.png" /></figure>
<table><caption>**Table 1.** Occurrence frequencies (%) of possible NoLS sequences (K/R-K/R-X-K/R) and of the twenty aminoacids at the X position in several NoLS-containing nucleolar proteins.</caption><thead><tr><th>Motif</th><th>KKXK</th><th>KKXR</th><th>KRXK</th><th>KRXR</th><th>RRXR</th><th>RRXK</th><th>RXKR</th><th>RKXK</th><th>OF<sup>a</sup></th><th>MF<sup>b</sup></th><th>OF/MF</th></tr></thead><tbody><tr><td>Res. X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>G</td><td>4.84</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>4.84</td><td>7.4</td><td>0.65</td></tr><tr><td>A</td><td>1.61</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1.61</td><td>7.4</td><td>0.22</td></tr><tr><td>V</td><td>1.61</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1.61</td><td>6.8</td><td>0.24</td></tr><tr><td>L</td><td>3.23</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1.61</td><td>-</td><td>4.84</td><td>7.6</td><td>0.64</td></tr><tr><td>I</td><td>-</td><td>-</td><td>-</td><td>1.61</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1.61</td><td>3.8</td><td>0.42</td></tr><tr><td>P</td><td>3.23</td><td>-</td><td>-</td><td>1.61</td><td>4.84</td><td>-</td><td>-</td><td>-</td><td>9.68</td><td>5.0</td><td>1.94</td></tr><tr><td>S</td><td>-</td><td>-</td><td>1.61</td><td>1.61</td><td>3.23</td><td>-</td><td>-</td><td>-</td><td>6.45</td><td>8.1</td><td>0.8</td></tr><tr><td>T</td><td>1.61</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1.61</td><td>6.2</td><td>0.26</td></tr><tr><td>C</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.00</td><td>3.3</td><td>0.00</td></tr><tr><td>M</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1.61</td><td>-</td><td>-</td><td>1.61</td><td>1.8</td><td>0.90</td></tr><tr><td>N</td><td>6.65</td><td>-</td><td>1.61</td><td>3.23</td><td>6.45</td><td>-</td><td>-</td><td>-</td><td>17.74</td><td>4.4</td><td>4.03</td></tr><tr><td>Q</td><td>-</td><td>1.61</td><td>-</td><td>-</td><td>3.23</td><td>-</td><td>-</td><td>-</td><td>4.84</td><td>3.7</td><td>1.31</td></tr><tr><td>F</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1.61</td><td>1.61</td><td>-</td><td>-</td><td>3.23</td><td>4.0</td><td>0.81</td></tr><tr><td>Y</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.00</td><td>3.3</td><td>0.00</td></tr><tr><td>W</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1.61</td><td>-</td><td>-</td><td>-</td><td>1.61</td><td>1.3</td><td>1.24</td></tr><tr><td>D</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.00</td><td>5.9</td><td>0.00</td></tr><tr><td>E</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.00</td><td>5.8</td><td>0.00</td></tr><tr><td>K</td><td>6.45</td><td>4.84</td><td>-</td><td>-</td><td>3.23</td><td>-</td><td>1.61</td><td>-</td><td>16.13</td><td>7.2</td><td>2.24</td></tr><tr><td>R</td><td>-</td><td>3.23</td><td>1.61</td><td>-</td><td>17.74</td><td>-</td><td>-</td><td>-</td><td>22.60</td><td>4.2</td><td>5.38</td></tr><tr><td>H</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.00</td><td>2.9</td><td>0.00</td></tr><tr><td></td><td>29.03</td><td>9.68</td><td>4.84</td><td>8.06</td><td>41.93</td><td>3.23</td><td>3.23</td><td>0.00</td><td></td><td></td><td></td></tr><tr><td></td><td>51.61</td><td></td><td></td><td></td><td>48.39</td><td></td><td></td><td></td><td></td><td></td><td></td></tr></tbody></table>

anti-rabbit (1:1000; Molecular Probes, Eugene, OR, USA), the Alexa Fluor 660 goat anti-human (1:1000; Invitrogen) or the Alexa Fluor 660 goat anti-mouse Igs (1:1000; Invitrogen). Each incubation was followed by three PBS washes and coverslips were mounted onto glass slides in Citifluor™ (Citifluor Ltd, Leicester, UK). When needed, cells were incubated 10 minutes with PBS containing 4',6-diamidino-2-phenylindole (DAPI, 0.1 µg.mL⁻¹) to visualize DNA and rinsed twice with PBS before mounting.

## Subcellular fractionation

Subcellular fractionation of HeLa and P19 cells was performed by differential centrifugation as described [41] with modifications in the buffer A composition (Tris-HCl 10 mmol.L⁻¹ pH 7.4, KCl 10 mmol.L⁻¹, MgCl₂ 1.5 mmol.L⁻¹, dithiothreitol 0.5 mmol.L⁻¹). Protein concentrations were determined using the Micro BCA Protein Assay Reagent Kit (Pierce, Rockford, IL, USA).

## 1D- and 2D-PAGE and Western blot

1D- and 2D-PAGE were performed as described [42,43] with minor modifications [44]. Electrotransfer of proteins onto nitrocellulose (Hybond C, GE Healthcare, Chalfont St Giles, UK) was performed according to [45] with minor modifications [44]. After membrane saturation [1], polyclonal anti-Ilf3/NF90 serum (Ab78; [1]), monoclonal anti-Ilf3/NF90 antibodies (BD Biosciences, Palo Alto, CA, USA), monoclonal anti-α-tubulin

antibodies (DM1A; Sigma-Aldrich), monoclonal anti-B23 antibodies (B0556; Sigma-Aldrich) or human polyclonal anti-UBF D165 serum were incubated overnight at room temperature and revealed with peroxidase-linked secondary antibodies (Sigma-Aldrich) by the chemiluminescence method.

## Plasmid constructions

Numbering of Ilf3 and NF90 primer oligodeoxynucleotides (MWG, Courtabœuf, France; Eurogentec, Liège, Belgium) was done according to the mouse Ilf3 and NF90 sequence published previously (GenBank accession numbers AF447751/NM010561, AF447752/NM001042707, DQ104405/NM001042708 and DQ104406/NM001042709; 5), italic types correspond to non-complementary sequences used to introduce specific restriction sites (underlined characters, *AGATCT*: BglII and *GAATTC*: EcoRI) or the Kosak consensus sequence (double-underlined characters, *GCCACC*), respectively.

To construct the short or long Ilf3/NF90 N-terminus in frame with the GFP (Short- or N- and Long- or NoLS-GFP), PCR were performed using plasmids pSK⁺ containing Ilf3 as template (5) and IN-51 (193-236: 5'-CCGGAATTCGCCACCATGGCATTGTATCATCATCACTTCATCACACAAGAAGAGAAGGCG-3') or IN-52 (193-229: 5'-CCGGAATTCGCCACCATGCGTCCCATGAATATTTTTGTGAATGATGATCGCC-3') as 5' primer and IN-31 (528-500 or 489-461: 5'-GGCTACCAGGCCGACCC-

<figure><img src="image_22.png" /></figure>

<footnote><sup>a</sup>Among the eight possible sequence combinations (upper line), observed frequency (OF) of each aminoacid at the X position in NoLS present in 61 well-characterized nucleolar proteins and using for the analysis.</footnote>

<footnote><sup>b</sup>Mean frequency (MF) of each aminoacid observed in vertebrate proteins.
doi:10.1371/journal.pone.0022296.t001</footnote>
GCATCACGCCCC-3') as 3' primer. Final PCR products were digested with EcoR I and Apa I, isolated in an 2.0% (w/v) agarose gel, electroeluted and finally subcloned in pEGFP-N1 (BD Biosciences) previously linearized with the same enzymes.

To construct the short or long N-terminus Ilf3/NF90 in frame with human eRF1 (N-heRF1 and NoLS-heRF1), PCR were performed using Long-GFP and Short-GFP plasmids as template and IN-53 (193-197: 5'-GCTCAAGCTTCGAATTCGCCACCATGGC-3') or IN-54 (193-197: 5'-GCTCAAGCTTCGAATTCGCCACCATGCG-3') as 5' primer and IN-32 (355-338 or 316-299: 5'-CATCCAGATCTGGGCCGCCTCAGTATGGG-3') as 3' primer. PCR products were digested with EcoR I and Bgl II, isolated by migration in an 1.5% (w/v) agarose gel, electroeluted and finally subcloned in pCMV-heRF1 [45] previously linearized with the same enzymes.

To construct the deletion and substitution NoLS-GFP mutants, site-directed mutagenesis PCR were performed using NoLS-GFP plasmid as template and primers containing some non-complementary nucleotides allowing to introduce mutation codons (bold types). To construct Δ4R-GFP mutant, Δ4R-5 (5'-ATGGCATTGTATCATCACTTCATCACACGTCCCATGAGATTTTTGAATGATGATCG-3') and Δ4R-3 (5'-CGATCATTCAAAAATTCTCATGGGACGTGTGATGAAGTGATGATGATACAATGCCAT-3') were used as 5' and 3' primers, respectively. To construct T10A-GFP and T10D-GFP mutants, T10A-5 (5'-GCATTGTATCATCATCACTTCATCGCAAGAGAAGAAGG-3') or T10D-5 (5'-GCATTGTATCATCATCACTTCATGATAGAAGAAGAAGG-3') and T10A-3 (5'-CCTTCTTCTTCTTGCGATGAAGTGATGATGATACAATGC-3') or T10D-3 (5'-CCTTCTTCTTCTATCGATGAAGTGATGATGATACAATGC-3') were used as 5' and 3' primers, respectively. To construct Δ3H-GFP, 3H->3A-GFP, 3H->3E-GFP, 3H->3F-GFP and 3H->3K-GFP mutants, Δ3H-5 (5'-CCGGAATTCGCCACCATGGCATTGTATTTTCATCACAAAGAAGAAGGCGTCCC-3') or 3H->3A-5 (5'-CCGGAATTCGCCACCATGGCATTGTATGCTGCAGCGTTTCATCACAAAGAAGAAGAAGGCGTCCC-3') or 3H->3E-5 (5'-CCGGAATTCGCCACCATGGCATTGTATGAGGAAGAGTTTCATCACAAAGAAGAAGAAGGCGTCCC-3') or 3H->3F-5 (5'-CCGGAATTCGCCACCATGGCATTGTATTTCTTCTTCTTCAATCACAAAGAAGAAGAAGGCGTCCC-3') or 3H->3K-5 (5'-CCGGAATTCGCCACCATGGCATTGTATAGAAGAAATTTCATCACAAAGAAGAAGAAGGCGT-3') and Δ3H-3 (5'-CGCCTTCTTCTTCTTGTGATGAAATACAATGCCATGGTGGCGAATTC-3') or 3H->3A-3 (5'-CGCCTTCTTCTTCTTGTGATGAAATACAATGCCATGGTGGCGAATTC-3') or 3H->3F-3 (5'-CGCCTTCTTCTTCTTCTTGTGATGAAAGAAGAAATACAATGCCATGGTGGCGAATTC-3') or 3H->3K-3 (5'-CGCCTTCTTCTTCTTGTGATGAATTTCTTCTTATACAATGCCATGGTGGCGAATTC-3') were used as 5' and 3' primers, respectively. To construct the 3H->3K/Δ4R-GFP double-mutant, site-directed mutagenesis PCR were performed using 3H->3K-GFP (see above) as template, and 3H->3K/Δ4R5 (5'-CCGGAATTCGCCACCATGGCATTGTATAAGAAGAAATTCATCACACGTCCCATGAG-3') and 3H->3K/Δ4R3 (5'-CTCATGGGACGTGTGATGAATTTCTTCTTATACAATGCCATGGTGGCGAATTC-3') as 5' and 3' primers, respectively. All products resulting from site-directed mutagenesis PCR were digested with Dpn I to discard methylated DNA template before transformation in bacteria.

To amplify expression vectors, constructs were introduced in *Escherichia coli* strain XL1 blue (Stratagene, Santa Clara, CA, USA)

then plasmids were purified using the QIAfilter Plasmid Midi Kit (Qiagen, Mayence, Germany).

## DNA transfection

DNA transfection in HeLa and P19 cells was performed by electroporation as described [46] or using Nanofectin reagent (PAA, Pasching, Austria). After 24 hours of culture, HeLa cells were fixed then used either for direct GFP fluorescence or indirect immunofluorescence analysis [1]. Indirect immunofluorescence was performed with monoclonal anti-B23 antibody FC82291 (Sigma-Aldrich), anti-fibrillin D157 human polyclonal serum (Dr Danièle Hernandez-Verdun' gift), rabbit serum S19 that recognizes eRF1 [46] and anti-α-tubulin DM1A monoclonal antibody (Sigma-Aldrich).

## Widefield microscopy

The different slides were observed using an upright BX41 Olympus microscope with 60X (1.25 N.A.) UPlan F1 APO objective. The DAPI was visualized with the DAPI/Hoechst/AMCA filter cube (Exc. 325-375, dic. 400, em. 435-485; Chroma Technology Corporation, Bellows Falls, VT, USA), the GFP or Alexa 488 fluorescence were detected with FITC filter cube (Exc. 465-495, dic. 505, em. 515-555; Chroma Technology Corporation, Bellows Falls, VT, USA), the Alexa 568 fluorescence was detected with mcherry filter cube (Exc. 530-560, dic. 570, em. 572-648; Chroma Technology Corporation, Bellows Falls, VT, USA) and the filter cube Y5 (exc. 630-650, dic. 660, em. 665-695; Chroma Technology Corporation, Bellows Falls, VT, USA) was used for visualization of Alexa 660. The different images were acquired using the CoolSnap Cf CCD camera (Photometrics, Roper Scientific, Tucson, AZ, USA) driven by Metamorph software (Molecular Devices, Sunnyvale, CA, USA).

## Confocal microscopy

The cells were imaged using confocal laser scanning microscopy (Leica SP2 or Leica SP5 system, Leica Microsystems, Heidelberg, Germany) using a HCX PL APO CS 63.0X 1.4NA oil UV objective. DAPI, GFP or Alexa 488, Alexa 568 and Alexa 660 were excited sequentially with 405, 488, 561 and 633 nm laser lines, respectively and the fluorescence were selected between 415-460, 500-535, 550-620 and 640-735 nm, respectively. The overlay images were done with ImageJ software.

## Photobleaching and live cell microscopy

24 hours after transfection, pre-warmed culture medium without phenol red and supplemented with fetal calf serum (10%, v/v) and Hepes (2 mmol.L⁻¹ pH 8.0) was added to HeLa cells. Coverslips were mounted in a Ludin chamber (Life Imaging Service, Basel, Switzerland) and cells were imaged with a Leica TCS-SP5 confocal laser scanning microscope (Leica, Solms, Germany) with a 63X 1.4NA oil UV objective. During experiments, cells were kept at 37°C using air conditioning chamber (Life Imaging Service). The regions of interest (ROI) were bleached with the 488 nm laser at full power whereas imaging acquisitions were done at 4% of laser full power. Images were collected in 512×512 format using the Leica LAS-AF software every two seconds during 40 seconds before, immediately after and at two seconds intervals after nucleolar bleaching for 240 seconds.

## Quantification of relative fluorescence intensity

Fluorescence intensity was measured using Image J 1.4g software. For FRAP experiments, the average intensity in the

<figure><img src="image_23.png" /></figure>
ROI before bleaching, immediately after bleaching and post-bleaching was measured. Fluorescence intensity of the nucleus was also measured. Background fluorescence was measured in a field outside the cell and subtracted from the nucleolar and nuclear fluorescence values. The normalized fluorescence intensity (FI) was calculated as follows: $FI = (IN_0/IN_t)/(IN_0/IN_0)$, where $IN_0$ corresponds to the average fluorescence intensity of the photo-bleached nucleolus at various time points after photobleaching, $IN_t$ to the average fluorescence intensity of the entire nucleus at the corresponding time point, $IN_0$ to the average fluorescence intensity of the photobleached nucleolus before photobleaching and $IN_0$ to the average fluorescence intensity of the entire nucleus before photobleaching.

Non-linear curve fitting of the recovery data was carried out with Microsoft Excel using the “Solver” macro. The fluorescence recovery was plotted against time and $t^{1/2}$ recovery was calculated using the following equations depending whether the diffusion corresponds to mono- or biexponential type: $F(t) = ((F_{inf} - F_0)(1 - e^{(-t/t_{1/2})}) + F_0)$ or $F(t) = ((F_{inf} - F_0)((1 - A)e^{(-t/t_{1/2,1})} - (1 - A)e^{(-t/t_{1/2,2})}) + F_0)$ where $F_0$ corresponds to the intensity value immediately after bleaching, $F_{inf}$ to the intensity value at the end of the experiment, $t$ to the time point and $A$ to one recovery fraction. All parameters were determined from the fitting of each

mean curve. The total mobile and the total immobile fractions were calculated on the fitted mean curves as described [47]. The total mobile fraction (MF) is given by $(F_{inf} - F_0) / (1 - F_0)$ and the immobile fraction (IF) by $1 - MF$.

## Acknowledgments

The authors thank Dr Danièle Hernandez-Verdun for helpful discussions and the gift of pEGFPN1-B23, mcherry-B23, pEGFPN1-fibrillarin plasmids, human polyclonal anti-fibrillarin D157 serum and human polyclonal anti-UBF D165 serum; Dr Samia Salhi and Dr Olivier Jean-Jean for the gift of pCMV-heRF1 plasmid and anti-eRF1 S19 rabbit serum; and Richard Schwartzmann from the Cell Imaging and Flow Cytometry facility of the IFR83 (Paris, France) for precious help in confocal microscopy study. We are grateful to Dr Dominique Dunon, Dr Annik Prat and Dr Nabil G. Seidah for helpful discussions and for the critical reading of the manuscript.

## Author Contributions

Conceived and designed the experiments: WV LG CD PD J-CL. Performed the experiments: WV LG AC VG J-CL. Analyzed the data: WV LG AC CD VG PD J-CL. Contributed reagents/materials/analysis tools: WV LG AC J-CL. Wrote the paper: PD J-CL.

## References

1. Larcher JC, Gasmi L, Viranaicken W, Eddé B, Bernard R, et al. (2004) Ilf3 and NF90 associates with the axonal targeting element of Tau mRNA. FASEB J 18: 1761-1763.

2. St Johnston D, Brown NH, Gall GJ, Jantsch M (1992) A conserved double-stranded RNA-binding domain. Proc Natl Acad Sci U S A 89: 10979-10983.

3. Duchange N, Pidoux J, Camus E, Sauvaget D (2000) Alternative splicing in the human interleukin enhancer binding factor 3 (ILF3) gene. Gene 261: 345-353.

4. Saunders LR, Perkins DJ, Balachandran S, Michaels R, Ford R, et al. (2001) Characterization of two evolutionarily conserved, alternatively spliced nuclear phosphoproteins, N-FAR-1 and -2, that function in mRNA processing and interact with the double-stranded-RNA-dependent protein kinase, PKR. J Biol Chem 276: 32300-32312.

5. Viranaicken W, Gasmi L, Chauvin C, Denoulet P, Larcher JC (2006) Identification of a newly spliced exon in the mouse Ilf3 gene generating two long and short isoforms of Ilf3 and NF90. Genomics 88: 622-632.

6. Tang J, Kao PN, Herschman HR (2000) Protein-arginine methyltransferase I, the predominant protein-arginine methyltransferase in cells, interacts with and is regulated by interleukin enhancer-binding factor 3. J Biol Chem 275: 19866-19876.

7. Patel RC, Vestal DJ, Xu Z, Bandyopadhyay S, Guo W, et al. (1999) DRBP76, a double-stranded RNA-binding nuclear protein, is phosphorylated by the interferon-induced protein kinase, PKR. J Biol Chem 274: 20432-20437.

8. Parker LM, Fierro-Monti I, Mathews MB (2001) Nuclear factor 90 is a substrate and a regulator of the eukaryotic initiation factor 2 kinase double-stranded RNA-activated protein kinase. J Biol Chem 276: 32522-32530.

9. Ting NS, Kao PN, Chan DW, Lintott LG, Lees-Miller SP (1998) DNA-dependent protein kinase interacts with antigen receptor response element binding proteins NF90 and NF45. J Biol Chem 273: 2136-2145.

10. Pei Y, Zhu P, Dang Y, Wu J, Yang X, et al. (2008) Nuclear export of NF90 to stabilize IL-2 mRNA is mediated by AKT-dependent phosphorylation at Ser467 in response to CD28 costimulation. J Immunol 180: 222-229.

11. Corthesy B, Kao PN (2000) Purification by DNA affinity chromatography of two polypeptides that contact the NF-AT DNA binding site in the interleukin 2 promoter. J Biol Chem 269: 20682-20690.

12. Reichman TW, Muñiz LC, Mathews MB (2002) The RNA binding protein nuclear factor 90 functions as both a positive and negative regulator of gene expression in mammalian cells. Mol Cell Biol 22: 343-356.

13. Reichman TW, Mathews MB (2003) RNA binding and intramolecular interactions modulate the regulation of gene expression by nuclear factor 110. RNA 9: 543-554.

14. Reichman TW, Parrott AM, Fierro-Monti I, Caron DJ, Kao PN, et al. (2003) Selective regulation of gene expression by nuclear factor 110, a member of the NF90 family of double-stranded RNA-binding proteins. J Mol Biol 332: 85-98.

15. Liao HJ, Kobayashi R, Mathews MB (1998) Activities of adenovirus virus-associated RNAs: purification and characterization of RNA binding proteins. Proc Natl Acad Sci U S A 95: 8514-8519.

16. Buaas FW, Lee K, Edelhoff S, Disteche C, Braun RE (1999) Cloning and characterization of the mouse interleukin enhancer binding factor 3 (Ilf3) homolog in a screen for RNA binding proteins. Mammal Genome 10: 451-456.

17. Shim J, Lim H, Yates JR, III, Karin M (2002) Nuclear export of NF90 is required for interleukin-2 mRNA stabilization. Mol Cell 10: 1331-1344.

18. Isken O, Grassmann CW, Sarisky RT, Kann M, Zhang S, et al. (2003) Members of the NF90/NFAR protein group are involved in the life cycle of a positive-strand RNA virus. EMBO J 22: 5655-5665.

19. Shi L, Zhao G, Qiu D, Godfrey WR, Vogel H, et al. (2005) NF90 regulates cell cycle exit and terminal myogenic differentiation by direct binding of the 3'-untranslated region of MyoD and p21<sup>WAF1/CIP1</sup> mRNAs. J Biol Chem 280: 18981-18989.

20. Parrott AM, Mathews MB (2007) Novel rapidly evolving hominid RNAs bind nuclear factor 90 and display tissue-restricted distribution. Nucleic Acid Res 35: 6249-6258.

21. Xu YH, Grabowski GA (1999) Molecular cloning and characterization of a translational inhibitory protein that binds to coding sequences of human beta-glucosidase and other mRNAs. Mol Genet Metab 68: 441-454.

22. Xu YH, Busald C, Grabowski GA (2000) Reconstitution of TCP80/NF90 translation inhibition activity in insect cells. Mol Genet Metab 70: 106-115.

23. Xu YH, Grabowski GA (2005) Translation modulation of acid β-glucosidase in HepG2 cells: participation of the PKC pathway. Mol Genet Metab 84: 252-264.

24. Kuwano Y, Pullmann R, Jr., Masara BS, Abdelmolhsen K, Lee EK, et al. (2010) NF90 selectively represses the translation of target mRNAs bearing an AU-rich signature motif. Nucleic Acid Res 38: 225-238.

25. Xu YH, Leonova T, Grabowski GA (2003) Cell cycle dependent intracellular distribution of two spliced isoforms of TCP/ILF3 proteins. Mol Genet Metab 80: 426-436.

26. Parrott AM, Walsh MR, Reichman TW, Mathews MB (2005) RNA binding and phosphorylation determine the intracellular distribution of nuclear factors 90 and 110. J Mol Biol 348: 281-293.

27. Brownawell AM, Macara IG (2002) Exportin-5, a novel karyopherin, mediates nuclear export of double-stranded RNA binding proteins. J Cell Biol 156: 53-64.

28. Weber JD, Kuo ML, Bothner B, DiGiammarino EL, Kriwacki RW, et al. (2000) Cooperative signals governing ARF-Mdm2 interaction and nucleolar localization of the complex. Mol Cell Biol 20: 2517-2528.

29. Horke S, Reumann K, Schweizer M, Will H, Heise T (2004) Nuclear trafficking of La protein depends on a newly identified nucleolar localization signal and the ability to bind RNA. J Biol Chem 279: 26563-26570.

30. Song Z, Wu M (2005) Identification of a novel nucleolar localization signal and a degradation signal in Survivin-deltaEx3: a potential link between nucleolus and protein degradation. Oncogene 24: 2723-2734.

31. Louvet E, Junéra HR, Le Panse S, Hernandez-Verdun D (2005) Dynamics and compartmentation of the nucleolar processing machinery. Exp Cell Res 304: 457-470.

32. Louvet E, Junéra HR, Berthuy I, Hernandez-Verdun D (2006) Compartmentation of the nucleolar processing proteins in the granular component is a CK2-driven process. Mol Biol Cell 17: 2537-2546.

33. Negi SS, Olson MO (2006) Effects of interphase and mitotic phosphorylation on the mobility and location of nucleolar protein B23. J Cell Sci 119: 3676-3685.

34. Hatanaka M (1990) Discovery of the nucleolar targeting signal. Bioessays 12: 143-148.

<figure><img src="image_24.png" /></figure>
35. Scott SS, Boisvert F-M, McDowall MD, Lamond AI, Barton GJ (2010) Characterization and prediction of protein nucleolar localization sequences. Nucleic Acid Res 38: 7388-7399.

36. Macchi P, Brownawell AM, Grunewald B, DesGroseillers L, Macara IG, et al. (2004) The brain-specific double-stranded RNA-binding protein Staufen2 - Nucleolar accumulation and isoform-specific exportin-5 dependent export. J Biol Chem 279: 31440-31444.

37. Sansam CL, Wells KS, Emeson RB (2003) Modulation of RNA editing by functional nucleolar sequestration of ADAR2. Proc Natl Acad Sci U S A 100: 14018-14023.

38. Kiebler MA, Jansen RP, Dahm R, Macchi P (2005) A putative nuclear function for mammalian Staufen. Trends Biochem Sci 30: 228-231.

39. Nie Y, Ding L, Kao PN, Braun R, Yang JH (2005) ADAR1 interacts with NF90 through double-stranded RNA and regulates NF90-mediated gene expression independently of RNA editing. Mol Cell Biol 25: 6956-6963.

40. Leung AK, Gerlich D, Miller G, Lyon C, Lam YW, et al. (2006) Quantitative kinetic analysis of nucleolar breakdown and reassembly during mitosis in live human cells. J Cell Biol 166: 787-800.

41. Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, et al. (2005) Nucleolar proteome dynamics. Nature 433: 77-83.

42. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.

43. O'Farell PA, Goodman HM, O'Farell P (1977) High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12: 1133-1142.

44. Larcher JC, Boucher D, Ginzburg I, Gros F, Denoulet P (1992) Heterogeneity of Tau proteins during mouse brain development and differentiation of cultured neurons. Develop Biol 154: 195-204.

45. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfert of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci U S A 76: 4350-4354.

46. Chauvin C, Salhi S, Le Goff C, Viranaicken W, Diop D, et al. (2005) Involvement of human eukaryotic release factors 3a and 3b in translation termination. Mol Cell Biol 25: 5801-5811.

47. Rabut G, Ellenberg J (2005) Photobleaching techniques to study mobility and molecular dynamics of proteins in live cells: FRAP, iFRAP, and FLIP. In: Goldman RD, Spector DL, eds. Live Cell Imaging: A Laboratory Manual Cold Spring Harbor Laboratory Press, Cold Spring Harbor volume 1: 101-127.

<figure><img src="image_25.png" /></figure>