# Validation of a Rapid Rabies Diagnostic Tool for Field Surveillance in Developing Countries

Monique Léchenne, Kemdongarti Naïssengar, Anthony Lepelletier, Idriss

Oumar Alfaroukh, Hervé Bourhy, Jakob Zinsstag, Laurent Dacheux

► To cite this version:

Monique Léchenne, Kemdongarti Naïssengar, Anthony Lepelletier, Idriss Oumar Alfaroukh, Hervé Bourhy, et al.. Validation of a Rapid Rabies Diagnostic Tool for Field Surveillance in Developing Countries. PLoS Neglected Tropical Diseases, 2016, 10 (10), 10.1371/journal.pntd.0005010. pasteur-01415805

HAL Id: pasteur-01415805

https://pasteur.hal.science/pasteur-01415805v1

Submitted on 13 Dec 2016

HAL is a multi-disciplinary open access
archive for the deposit and dissemination of sci-
entific research documents, whether they are pub-
lished or not. The documents may come from
teaching and research institutions in France or
abroad, or from public or private research centers.

L'archive ouverte pluridisciplinaire HAL, est
destinée au dépôt et à la diffusion de documents
scientifiques de niveau recherche, publiés ou non,
émanant des établissements d'enseignement et de
recherche français ou étrangers, des laboratoires
publics ou privés.

<figure><img src="image_1.png" /></figure>

Distributed under a Creative Commons CC BY 4.0 - Attribution - International License
<figure><img src="image_2.png" /></figure>

CrossMark

click for updates

RESEARCH ARTICLE

# Validation of a Rapid Rabies Diagnostic Tool for Field Surveillance in Developing Countries

Monique Léchenne¹,²*, Kemdongarti Naïssengar³, Anthony Lepelletier⁴, Idriss Oumar Alfaroukh³, Hervé Bourhy⁴, Jakob Zinsstag¹,², Laurent Dacheux⁴*

1 Swiss Tropical and Public Health Institute, Basel, Switzerland, 2 University of Basel, Basel, Switzerland, 3 Institut de Recherche en Elevage pour le Developpement, Farcha, N'Djamena, Chad, 4 Institut Pasteur, Unit Lyssavirus Dynamics and Host Adaptation, National Reference Center for Rabies and WHO Collaborating Centre for Reference and Research on Rabies, Paris, France

* monique.lechenne@unibas.ch (ML); laurent.dacheux@pasteur.fr (LD)

## Abstract

### Background

One root cause of the neglect of rabies is the lack of adequate diagnostic tests in the context of low income countries. A rapid, performance friendly and low cost method to detect rabies virus (RABV) in brain samples will contribute positively to surveillance and consequently to accurate data reporting, which is presently missing in the majority of rabies endemic countries.

### Methodology/Principal findings

We evaluated a rapid immunodiagnostic test (RIDT) in comparison with the standard fluorescent antibody test (FAT) and confirmed the detection of the viral RNA by real time reverse transcription polymerase chain reaction (RT-qPCR). Our analysis is a multicentre approach to validate the performance of the RIDT in both a field laboratory (N'Djamena, Chad) and an international reference laboratory (Institut Pasteur, Paris, France). In the field laboratory, 48 samples from dogs were tested and in the reference laboratory setting, a total of 73 samples was tested, representing a wide diversity of RABV in terms of animal species tested (13 different species), geographical origin of isolates with special emphasis on Africa, and different phylogenetic clades. Under reference laboratory conditions, specificity was 93.3% and sensitivity was 95.3% compared to the gold standard FAT test. Under field laboratory conditions, the RIDT yielded a higher reliability than the FAT test particularly on fresh and decomposed samples. Viral RNA was later extracted directly from the test filter paper and further used successfully for sequencing and genotyping.

### Conclusion/Significance

The RIDT shows excellent performance qualities both in regard to user friendliness and reliability of the result. In addition, the test cassettes can be used as a vehicle to ship viral RNA to reference laboratories for further laboratory confirmation of the diagnosis and for epidemiological investigations using nucleotide sequencing. The potential for satisfactory use in

<figure><img src="image_3.png" /></figure>

**OPEN ACCESS**

**Citation:** Léchenne M, Naïssengar K, Lepelletier A, Alfaroukh IO, Bourhy H, Zinsstag J, et al. (2016) Validation of a Rapid Rabies Diagnostic Tool for Field Surveillance in Developing Countries. PLoS Negl Trop Dis 10(10): e0005010. doi:10.1371/journal.pntd.0005010

**Editor:** Charles E Rupprecht, Wistar Institute, UNITED STATES

**Received:** December 21, 2015

**Accepted:** August 29, 2016

**Published:** October 5, 2016

**Copyright:** © 2016 Léchenne et al. This is an open access article distributed under the terms of the [Creative Commons Attribution License](http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

**Data Availability Statement:** All relevant data are within the paper and its Supporting information files.

**Funding:** This study has been kindly funded by the Federal Food Safety and Veterinary Office, Switzerland (project number 1.11.07). This study was also supported in part by Santé publique France (formerly Institut de Veille Sanitaire-InVS) and by the Direction Générale de la Santé, France. The funders had no role in study design, data collection and analysis,
decision to publish, or preparation of the
manuscript.

**Competing Interests:** The authors have declared
that no competing interests exist.

remote locations is therefore very high to improve the global knowledge of rabies epidemiol-
ogy. However, we suggest some changes to the protocol, as well as careful further valida-
tion, before promotion and wider use.

Author Summary

The high fatality and burden of rabies stands in contrast to the very low performance of labo-
ratory-based surveillance in resource-challenged countries. The absence of reliable human
and animal rabies incidence data ultimately result in neglect of disease prevention and control
and the perpetuation of RABV transmission despite the existence of powerful management
tools. Rapid, easy to perform rabies diagnostic tests that do not require expensive equipment
or special storage conditions, which can be reliably performed by trained ordinary veterinary
professionals, are needed urgently for use in low income countries. Such novel methods will
help to accurately assess the global rabies burden and are necessary to monitor rabies control
and elimination. The present study evaluates the performance and reliability of a rapid, easy
to use rabies diagnostic tool. Overall, the validated test was in high accordance with the stan-
dard reference method for the detection of RABV by immunofluorescence microscopy and
showed even higher reliability when applied in resource poor laboratory conditions. The
obtained results support the high potential for the use of this test in the field but suggest a
change of the original technical protocol and a need for wider validation.

**Introduction**

Rabies is a viral zoonotic encephalomyelitis transmitted to humans after exposure to infected mammals, mainly dogs, through bites, scratches or licks on damaged skin or mucous membranes. This disease still continues to represent a public health concern worldwide, with an estimate of 60,000 human deaths per year, mainly in low income countries. Because of limited control measures in many countries and a lack of governmental concern, rabies remains a neglected tropical disease. This neglect is especially deplorable given the entirely preventable nature of the disease through vaccination of dogs and timely adherence to post-exposure prophylaxis (PEP) of exposed victims [1, 2]. In this way, human deaths due to this zoonotic disease could be reduced by over 95% [3, 4].

Lack of surveillance represents one major element of negligence, leading to missing data on
disease incidence, imprecise estimates of the economic impact and a general underestimation
of the true worldwide burden of rabies [4, 5, 6, 7]. This means that advocacy for rabies control
cannot be supported with solid evidence and the necessity for action is not perceived at the
decision maker level, for instance governmental authorities [8]. Poor surveillance is primarily a
result of lack of political commitment and resource attribution for the control of rabies, and
thus a cycle of neglect perpetuates. The vicious cycle is reinforced by the disregard of disease
control in domestic dogs, which constitute negligible economic value and the fact that rabies
affects largely marginalized communities with difficult access to healthcare. Deficiencies in
basic healthcare do not only contribute to hinder access to PEP but also lead to the misdiago-
sis of rabies in the face of other causes of encephalitis, such as cerebral malaria, as reported in
Malawi [9]. Surveillance is fundamental to accurate burden of disease measures, for advocacy
of disease control and also a prerequisite for disease elimination [10, 11]. Currently, rabies is
believed to be underreported at the extent of 1:60 in humans and this rate could even be much
higher for animal rabies incidence [6].
One possible point of leverage to break the cycle of underreporting and neglect is the rein-
forcement and simplification of diagnostic capacities and tools. Infrastructures required for the
current standard diagnostic tests are expensive, and their methodologies and interpretation need
thoroughly experienced personnel. Antigen detection of RABV using the direct fluorescent anti-
body test (FAT) is the World Health Organization (WHO) and World Organisation for Animal
Health (OIE) reference test [6, 12, 13] and is routinely performed in many developed countries.
However, it is difficult to establish in developing countries because fluorescence microscopes are
expensive and the required maintenance is demanding. Also, the immunofluorescence conjugate
necessary for the test is costly and has to be transported and stored refrigerated. Finally, accurate
reading of the test needs stringent quality control of the test performance and very experienced
personnel. Similar constraints are encountered with the direct rapid immunohistochemical test
(DRIT) [14, 15]. Although the DRIT can be read using a light microscope, the test methodology
requires a meticulous protocol which currently lacks commercialized biotinylated anti-rabies
antibodies and has to be carried out by trained personnel. For rabies diagnosis however, a simpler
field test is desirable for various reasons. In the current situation the benefits of rabies diagnosis
are not well perceived by the public and rabies suspicious animals are often killed immediately
and rapidly disposed [16]. Also, a short time lag between suspicion and confirmation of a rabies
case is important for early adherence to PEP or the cost savings in case of a negative diagnostic
result. Finally, transport of samples over long distances in climatically warm settings increases
the risk of poor sample quality, which adversely affects FAT test results [17]. Proximity to the
public through decentralized laboratory facilities is therefore vital for good sample quality, as well
as rapid detection and response.

A rapid immunodiagnostic test (RIDT) based on the lateral flow principle was first described and evaluated in 2007 on a limited panel of RABV samples [18]. The same study reported on the detection limit and potential risk of cross reactivity. Further laboratory evalua- tion was conducted more recently on the use of this RIDT for the detection of RABV circulat- ing in Europe, and extended to the detection of other species of lyssaviruses [19, 20]. Both studies showed positive results regarding sensitivity and specificity of such tests compared to the FAT. To date, only two studies have been conducted under field conditions, both suggest- ing positive results for the use of RIDT [21, 22].

In our study, we evaluated the practicability and the performance of this RDIT identified as
Anigen Rapid Rabies Test (Anigen test) (Bionote Inc.) in different settings: under field condi-
tions with its application to the surveillance of rabid animals in N'Djamena, Chad and in labo-
ratory settings with a panel of selected RABV isolates. Lastly, we evaluated this tool for a novel
application in rabies surveillance, with its use as a vehicle for viral RNA storage and conserva-
tion, and demonstrated that recovery and detection of RNA present on the strip of positive
samples was possible. The Anigen test appears as a promising tool for the *post-mortem* diagno-
sis of animal rabies, and the molecular detection and genotyping of positive test strips.

**Materials and Methods**

Implementation of the Anigen test in the field

During June 2012, the RIDT Anigen test, a chromatographic immunoassay-based on lateral flow technology manufactured by BioNote, Inc (Gyeongi-do, Republic of Korea) [23], was added into the routine diagnostic procedure of the rabies laboratory of the Institut de Recherche en Elevage pour le Développement (IRED) in N'Djamena, Chad. It was utilized in parallel with the FAT test, which had been used since 2001. Rabies-suspect animals were presented to the IRED by their owners or by the bite victim. No active surveillance was initiated throughout the study. However, awareness was intensified prior to the study period, during May 2012, by a
poster campaign sensitizing the public in N'Djamena to seek medical treatment after a dog bite and to send the biting animal to the IRED in case of rabies suspicion.

## Description of samples and isolates

For the validation of the Anigen test in the field, diagnostic results from June 2012 to February 2015 were included. Only samples originating from dogs were considered for inclusion according to the manufacturer's recommendation [23]. During the 33 months of the study period, a total of 49 rabies-suspect dog heads were submitted to IRED for diagnostic testing. The origin of the samples is detailed in Table 1. Most were in fresh condition on arrival and upon testing (85%, n = 42). Five of the samples were decomposed, while in one case, the sample quality was not noted. Only one sample was so decomposed that it was impossible to analyse and was excluded from the study. The final sample size of field isolates at IRED was 48 (Table 1).

The Anigen test was further validated at the National Reference Centre (NRC-R) and WHO Collaborating Center for Rabies at the Institut Pasteur in Paris, France, on 73 samples selected from the collections housed in both of these centers, from 12 different species originating from various countries and belonging to different phylogenetic clades (S1 Table). All these samples were previously analysed by FAT. Thirty of them were negative and the remaining 43 were positive. The positive samples represented a large diversity of RABV. All these 73 samples were stored at -80°C for archive before analysis.

In addition, the limit of detection of the RIDT was evaluated at NRC-R using a panel of 8 different isolates of RABV adapted and amplified on baby hamster kidney cells (BSR cells). Viral suspensions were titrated on the same cells using 5-fold serial dilutions in cell culture medium and expressed as fluorescent focus units per mL (FFU/mL). For the RIDT evaluation, titrated RABV suspensions were first tested at several concentrations using the buffer available from the RIDT kit as a diluent.

## Description of the FAT methodology

The FAT, the gold standard technique for post-mortem diagnosis of rabies [12] was performed at the NRC-R under quality assurance (accreditation ISO/IEC 17025), as previously described [13]. In the rabies laboratory of N'Djamena, the FAT was performed with some deviations regarding the standard procedure: lack of positive and negative control samples inclusion, absence of routine quality assessments, and storage of the immunofluorescent conjugate past the expiration date. In this setting, two microscopic slides were prepared, with two brain impressions per slide. If no viral characteristic fluorescent inclusions were observed on all four impressions, the sample was considered negative. Doubtful results were declared positive due to the potential fatal consequences of a false negative result for the bite victims. However, due to some deviations regarding the standard procedure, it was not possible to consider FAT performed at IRED as the gold standard for the specificity and sensitivity analysis.

## Description of the RIDT Anigen test methodology

The Anigen test is a simple and rapid diagnostic tool, presenting as an all-in-one included kit. Once the brain is extracted, it is used without additional material and equipment except for one dilution step requiring an additional vial of phosphate-buffered saline (PBS) prepared according to the manufacturer's recommendation. However, for our study, we omitted the first dilution step, only using the vial with buffer provided by the kit to simplify the test procedure in view of future application under realistic field conditions. The same procedure was used for the Anigen test at NRC-R and at IRED. If it was possible to anatomically identify the regions of the brain in a sample, the test was performed with a small section of the brainstem
<table><caption>**Table 1.** Description of samples tested in Chad and results obtained after FAT, RIDT and RT-qPCR on FTA papers. All specimens correspond to original brain samples collected from dogs.</caption><thead><tr><td>Identification</td><td>Sampling date</td><td>Origin</td><td>Condition</td><td>FAT Result</td><td>RIDT Result</td><td>RT-qPCR Result</td></tr></thead><tbody><tr><td>342</td><td>06.06.2012</td><td>N'Djamena</td><td>Fresh</td><td>Neg</td><td>Neg</td><td>Neg</td></tr><tr><td>343</td><td>21.06.2012</td><td>N'Djamena</td><td>Decomposed</td><td>Pos</td><td>Neg</td><td>Neg</td></tr><tr><td>344</td><td>22.06.2012</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>345</td><td>28.06.2012</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>346</td><td>05.07.2012</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>347</td><td>13.07.2012</td><td>Koundoul</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>348</td><td>20.07.2012</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>349</td><td>31.07.2012</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>350</td><td>08.08.2012</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>352</td><td>25.08.2012</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>354</td><td>29.08.2012</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>355</td><td>11.09.2012</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>356</td><td>12.09.2012</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>357</td><td>20.09.2012</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>358</td><td>25.09.2012</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>359</td><td>28.09.2012</td><td>unknown</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>360</td><td>01.10.2012</td><td>N'Djamena</td><td>Fresh</td><td>Neg</td><td>Neg</td><td>Neg</td></tr><tr><td>361</td><td>26.10.2012</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>362</td><td>24.12.2024</td><td>unknown</td><td>Unknown</td><td>Pos</td><td>Neg</td><td>Neg</td></tr><tr><td>363</td><td>19.11.2012</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>364</td><td>03.12.2012</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>365</td><td>05.12.2012</td><td>N'Djamena</td><td>Fresh</td><td>Neg</td><td>Neg</td><td>Neg</td></tr><tr><td>366</td><td>06.12.2012</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>367</td><td>10.01.2013</td><td>Moundou</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>368</td><td>11.01.2013</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>369</td><td>08.02.2013</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>371</td><td>25.03.2013</td><td>Koundoul</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>372</td><td>29.05.2013</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>373</td><td>17.06.2013</td><td>Koundoul</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>379</td><td>12.11.2013</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>380</td><td>12.12.2013</td><td>N'Djamena</td><td>Fresh</td><td>Neg</td><td>Neg</td><td>Neg</td></tr><tr><td>381</td><td>31.01.2014</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>383</td><td>16.04.2014</td><td>N'Djamena</td><td>Fresh</td><td>Neg</td><td>Neg</td><td>ND</td></tr><tr><td>384</td><td>29.04.2014</td><td>N'Djamena</td><td>Fresh</td><td>Neg</td><td>Neg</td><td>ND</td></tr><tr><td>386</td><td>29.04.2014</td><td>Guelendeng</td><td>Fresh</td><td>Neg</td><td>Neg</td><td>ND</td></tr><tr><td>389</td><td>28.05.2014</td><td>N'Djamena</td><td>Decomposed</td><td>Imp</td><td>Neg</td><td>Neg</td></tr><tr><td>390</td><td>06.06.2014</td><td>N'Djamena</td><td>Fresh</td><td>Neg</td><td>Neg</td><td>ND</td></tr><tr><td>392</td><td>23.06.2014</td><td>N'Djamena</td><td>Decomposed</td><td>Neg</td><td>Neg</td><td>ND</td></tr><tr><td>393</td><td>14.07.2014</td><td>Koundoul</td><td>Fresh</td><td>Neg</td><td>Neg</td><td>ND</td></tr><tr><td>394</td><td>18.08.2014</td><td>Loumia</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>395</td><td>21.08.2014</td><td>Chawaye</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>400</td><td>13.10.2014</td><td>N'Djamena</td><td>Fresh</td><td>Neg</td><td>Neg</td><td>ND</td></tr><tr><td>401</td><td>14.10.2014</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>403</td><td>09.12.2014</td><td>Loumia</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>405</td><td>27.01.2015</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>406</td><td>05.02.2015</td><td>Djermaya</td><td>Decomposed</td><td>Pos</td><td>Pos</td><td>ND</td></tr></tbody></table>

(Continued)
<table><caption>**Table 1.** (Continued)</caption><thead><tr><td>Identification</td><td>Sampling date</td><td>Origin</td><td>Condition</td><td>FAT Result</td><td>RIDT Result</td><td>RT-qPCR Result</td></tr></thead><tbody><tr><td>407</td><td>11.02.2015</td><td>Dourbali</td><td>Decomposed</td><td>Pos</td><td>Pos</td><td>Pos</td></tr><tr><td>408</td><td>13.02.2015</td><td>N'Djamena</td><td>Fresh</td><td>Pos</td><td>Pos</td><td>Pos</td></tr></tbody></table>

Pos: positive, Neg: negative, Imp: impossible, ND: not done.

doi:10.1371/journal.pntd.0005010.t001

(approximately 0.1 g), otherwise the same amount of material was taken from different parts of decomposed brain samples. The brain sample was mixed directly in the tube containing the buffer with the swab, all included in the kit, for about one minute, until most of the brain material was well dissolved and then put on to the test plate using the transfer pipette provided in the kit. Four drops were deposited on the strip (corresponding to nearly 100 µL). The test could be interpreted when the coloured liquid reached the top of the test and the purple indicator colour had vanished from the filter paper background. As described by the manufacturer, a positive test result was indicated by two purple lines, one in the test zone and the other in the control zone. If a line only appeared in the control zone, the test was considered negative. In cases where only the test line was coloured rather than the control band, the test was declared invalid and was performed once again. The test took approximately 5 to 10 min after deposit of sample and the interpretation was not be performed after 10 min, according to manufacturer recommendations. Following these recommendations, the test was suitable for dog, raccoon dog and cattle samples (animals which were used originally in the validation of the method [18], and should be tested immediately after collection. In this study, two different batches were used for the validation of the RIDT assay, with batches n°1801076 and n°1801111 for the field and the laboratory validation, respectively.

## Extraction of RNA from Anigen test and FTA Whatman cards

Brain impressions were performed directly on FTA Whatman cards, a support dedicated to the storage and preservation of RNA [24]. Prior to use, the cards were stored at room temperature in a sealed plastic bag in a dry and clean area. The samples were prepared by diluting a small section (approximately 0.1 g) of the brain in 1 ml PBS (10%). After thorough mixing, the brain homogenate was loaded onto the card with a pipette until the sample indicator circle on the filter was covered. The cards were then dried 24 hours at room temperature before being put separately in transparent plastic bags for transportation.

To prepare the samples on FTA Whatman filter paper, 1 cm² was cut-off from the area containing the brain impression and incubated during 1 hour in Tri-Reagent LS (Molecular Research Center, Cincinnati, Ohio, USA) or overnight in cell culture medium (DMEM) (Life Technologies, Saint Aubin, France), then placed in Tri-Reagent. To obtain viral RNA directly from the Anigen test strip, the cassettes were opened, the filter paper was removed and the area where the sample was deposited was collected and placed into 1 mL of Tri-Reagent LS. For both FTA and Anigen test supports, total RNA extraction was performed as previously described, following manufacturer recommendations [25].

## Viral RNA detection by RT-qPCR

Viral RNA detection was performed using a one-step dual combine pan-lyssavirus RT-qPCR assay recently described [26], targeting a conserved region among the polymerase. Briefly, this assay includes a pan-RABV RT-qPCR probe-based technique, able to detect all representatives of the broad genetic diversity of RABV, using two degenerated TaqMan probes. In parallel, a
SYBR Green RT-qPCR assay is able to detect all the other lyssaviruses tested, in addition to RABV isolates. Both of these assays, which were optimized to a final reaction volume of 20 µL, were performed using 5 µL of RNA template (previously diluted 1:10 in nuclease-free water). For each assay, appropriate controls were used. Details of the combined pan-lyssavirus RT-qPCR assay are in S2 Table and in reference [26].

## Evaluation of viral RNA extracted from Anigen test strips for genotyping

A selected panel of RNA extracts from Anigen test, which were found positive with the dual combine RT-qPCR assay, were evaluated with RT-PCR to generate amplicons suitable for genotyping by sequencing (at least 500 nt in length). Briefly, a volume of 6 µL of total RNA extraction was used for reverse transcription as previously described [25]. RNA was incubated at 65°C for 10 min with 2 µL of pd(N)₆ random primers (200 µg/mL; Roche Diagnostics) and 2 µL of sterilized distilled water and then were stored on ice. Each tube was incubated with 200 U of Superscript II RT (Invitrogen), 80 U of RNasin (Promega), and 10 nmol of each nucleotide triphosphate (Eurobio), in a final volume of 30 µL for 90 min at 42°C, for reverse transcription. Two microliters of complementary DNA (cDNA) were then amplified by PCR targeting the nucleoprotein gene of RABV, as described in [27].

## Evaluation of the Anigen test in an inter-laboratory trial

The RIDT assay was evaluated by the NRC-R in an inter-laboratory trial organized during 2015 by the European Union reference laboratory for rabies, which is located in Nancy, France [28]. The FAT technique was also evaluated in parallel in this trial. The test panel consisted of nine anonymous samples of freeze-dried homogenized brains, either uninfected or infected with various lyssavirus species. Details of this trial have been provided elsewhere [28].

## Statistical analysis

Results obtained with FAT and Anigen techniques were compared using the McNemar and Kappa statistic tests in Stata, and were analyzed to determine the intrinsic parameters of the RIDT assay. However and conversely to the FAT technique done at the NRC-R, the immuno-fluorescence assay performed at the IRED could not be considered as the reference technique due to several deviations compared to the standard procedure. In case of discrepancy between RIDT and FAT, samples were tested for RNA detection with RT-qPCR assay performed on FTA Whatman cards impregnated with the brain of the corresponding sample. For the determination of the sensitivity and the specificity of RIDT, true positivity and true negativity was defined according to the result that was shared by at least two tests among FAT, RIDT and viral RNA detection.

## Results

### RIDT test processing and interpretation

For the majority of the total sample size (n = 121) tested at IRED and at NRC-R, the RIDT was successfully performed, with the presence of a line clearly visible in the control zone after 5 to 15 min of migration once the sample was deposited (Fig 1A and 1B). For only a few samples (n<10), the test was repeated, due to abnormal or incomplete migration (absence of the line in the control zone). When they scored positive with RIDT, most samples exhibited a line with strong intensity in the test zone (Fig 1B). In a few cases the test bands showed even higher intensity than the control band. Also, for some samples tested at NRC-R, the line in the test area was only faintly visible, despite a strong intensity of the line in the control zone (Fig 1A).
A

<figure><img src="image_4.png" /></figure>

B

<figure><img src="image_5.png" /><figcaption>**Fig 1. Examples of RIDT results.** (A) Results from NRC-R, with six positive results including the two middle cassettes (samples 93101 and 87006) demonstrating only a weak test line in the results window. (B) Results from Chad, with one negative result (sample 18/12) and four positive results. The upper line visible in the results window is the control (C) and the lower line represents the test (T). The presence of two lines depicts a positive result, whereas the presence of only the upper line indicates a negative result. The test is considered as invalid when the upper line is not visible (not shown).</figcaption></figure>

doi:10.1371/journal.pntd.0005010.g001

## Determination of the limit of detection of the RIDT

A total of eight titrated suspensions from different RABV adapted to cell culture was selected to determine the limit of detection of the RIDT (Table 2). A volume of 100 µL of each of them, diluted or not, were tested. The lowest number of fluorescent focus-forming units (FFU) detected with this assay was 10⁵ FFU, and was obtained for RABV 9704ARG and 04030PHI.
<table><caption>Table 2. Limit of detection of the RIDT using 8 different titrated rabies virus suspensions.</caption><thead><tr><th>Virus strain<sup>a</sup></th><th>Original host</th><th>Location</th><th>Initial virus concentration (FFU/mL)<sup>b</sup></th><th>Limit of detection (FFU)<sup>c</sup></th></tr></thead><tbody><tr><td>9147FRA</td><td>Red fox</td><td>France</td><td>3.1 x 10<sup>7</sup></td><td>10<sup>6</sup></td></tr><tr><td>CVS</td><td>Lab isolate</td><td>-</td><td>1.6 x 10<sup>7</sup></td><td>10<sup>6</sup></td></tr><tr><td>8743THA</td><td>Human</td><td>Thailand</td><td>8.1 x 10<sup>7</sup></td><td>&gt; 8.1 x 10<sup>6</sup></td></tr><tr><td>9508CZK (SAD)</td><td>Lab isolate</td><td>-</td><td>5.4 x 10<sup>8</sup></td><td>10<sup>7</sup></td></tr><tr><td>PV</td><td>Lab isolate</td><td>-</td><td>4.3 x 10<sup>7</sup></td><td>10<sup>6</sup></td></tr><tr><td>9001FRA</td><td>Dog</td><td>French Guiana</td><td>2.4 x 10<sup>6</sup></td><td>&gt; 2.4 x 10<sup>5</sup></td></tr><tr><td>9704ARG</td><td>Bat</td><td>Argentina</td><td>9.5 x 10<sup>7</sup></td><td>10<sup>5</sup></td></tr><tr><td>04030PHI</td><td>Human</td><td>Philippines</td><td>2.5 x 10<sup>7</sup></td><td>10<sup>5</sup></td></tr></tbody></table>

doi:10.1371/journal.pntd.0005010.t002

Isolates 9147FRA and 9508CZK exhibited a positive signal with 10⁶ FFU. Lastly, no positive signal was obtained with the initial viral suspension for virus 8743THA and 9001FRA, indicating that the limit of detection was > 8.1 x 10⁶ FFU and > 2.4 x 10⁵ FFU, respectively.

## Comparison results between FAT and RIDT

Seventy-three samples from NRC-R, including forty-three positive samples representing a large diversity in term of host species, geographical location and genetic diversity (S1 and S3 Tables) were tested. Compared to the gold standard FAT, the RIDT demonstrated an accordance of 95%. The specificity was 93.3% with only two false positive results among the 30 FTA-negative specimens, noticed for samples 150057 and 150125 which were originated from a dog and a cat, respectively (Table 3, S1 and S3 Tables). The sensitivity of the RIDT was 95.3%, with only two false negative results observed for isolates 9217ALL and 9312MAU, a red fox from Germany and a dog from Mauritania, respectively (Table 3, S1 and S3 Tables).

Among the 48 samples included for evaluation at IRED only 3 were not concordant between FAT and RIDT, yielding an accordance of 94% (Table 3). Two of the discordant samples

<table><caption>Table 3. Intrinsic parameters of the RIDT after laboratory evaluation (NRC-R), under field conditions (IRED) and when combining both conditions (all).</caption><thead><tr><th rowspan="2"></th><th rowspan="2"></th><th rowspan="2"></th><th colspan="4">FAT results</th></tr><tr><th>Pos</th><th>Neg</th><th>Imp</th><th>Total</th></tr></thead><tbody><tr><td rowspan="3">NRC-R</td><td>Pos</td><td></td><td>41</td><td>2</td><td>0</td><td>43</td></tr><tr><td>Neg</td><td></td><td>2</td><td>28</td><td>0</td><td>30</td></tr><tr><td>Total</td><td></td><td>43</td><td>30</td><td>0</td><td>73</td></tr><tr><td rowspan="3">IRED</td><td>Pos</td><td></td><td>34</td><td>0</td><td>0</td><td>34</td></tr><tr><td>Neg</td><td></td><td>2</td><td>11</td><td>1</td><td>14</td></tr><tr><td>Total</td><td></td><td>36</td><td>11</td><td>1</td><td>48</td></tr><tr><td rowspan="3">All</td><td>Pos</td><td></td><td>75</td><td>2</td><td>0</td><td>77</td></tr><tr><td>Neg</td><td></td><td>4</td><td>39</td><td>1</td><td>44</td></tr><tr><td>Total</td><td></td><td>79</td><td>41</td><td>1</td><td>121</td></tr></tbody></table>

doi:10.1371/journal.pntd.0005010.t003

<footnote><sup>a</sup> CVS: Challenge virus strain, SAD: Street Alabama Dufferin, PV: Pasteur virus.</footnote>

<footnote><sup>b</sup> Number of fluorescent focus-forming units (FFU) per mL.</footnote>

<footnote><sup>c</sup> Number of fluorescent focus-forming units (FFU) deposited on the strip.</footnote>
(samples 343 and 389) were decomposed and the quality remained unknown for the last one (sample 362) (Table 1). The FAT was impossible to perform on one (sample 389) of the 3 and for the two others (samples 343 and 362), the result was positive (S4 Table). For all these 3 specimens, RIDT tested negative (S4 Table). In these three cases, where RIDT and FAT did not yield the same result, viral detection performed by RT-qPCR on the FTA Whatman card could not detect viral RNA after multiple attempts confirming the negative RIDT result (S4 Table). For sensitivity and specificity of RIDT and FAT under field conditions, true positivity and true negativity was defined according to the result that was shared by at least two tests among FAT, RIDT and viral RNA detection on the FTA Whatman card. The RIDT showed a higher specificity (100%) than the FAT (78.5%) at IRED. Accordance with the overall true test results of the 48 samples from IRED was 100% for RIDT and 94% for FAT.

The McNemar test showed no significant difference between the FAT and RIDT (Exact McNemar significance probability = 0.5) on samples test at IRED and the Kappa value was 0.86, indicating excellent agreement between the tests. The exact McNemar significance probability for the comparison of FAT and RIDT performed at NRC-R was 1 and the Kappa value was 0.89. Overall, of the 121 samples analysed at NRC-R and IRED, the McNemar significance probability was found to be 0.45 and the Kappa value was 0.87.

## Evaluation of viral RNA detection by RT-qPCR using Anigen test strips and comparison with results obtained using FTA Whatman cards

A total of 51 samples were tested at NRC-R for viral RNA detection using RT-qPCR on the Anigen test strip, which were previously found positive for the *post-mortem* diagnosis of rabies (Table 4, S1 and S4 Tables). The FAT scored also positive for all of these specimens. Among them, 32 originating from IRED were used during the field evaluation of the RIDT, whereas 19 were obtained in NRC-R during the laboratory evaluation. Positive detection was obtained for 26 (81.2%), 18 (94.7%) and 44 (86.3%) samples from IRED, NRC-R and the two combined, respectively (Table 4). In parallel, detection of viral RNA was also performed at NRC-R on FTA Whatman cards for 31 samples, which were positive after analysis with both FAT and RIDT at IRED (S4 Table). In this case, a perfect concordance (100%) was noticed. In addition, viral RNA from 2 and 6 samples found previously negative with RIDT were not detected after RT-qPCR analysis performed on the Anigen test strips and FTA Whatman cards, respectively (Table 4). When compared to the FTA Whatman card, RT-qPCR performed on the Anigen test strip exhibited a sensitivity of 80.6% (Table 5).

## Evaluation of the use of viral RNA for genotyping after extraction from Anigen test strips

A limited panel of viral RNA samples extracted from Anigen test strips, which were previously confirmed positive by RT-qPCR, were secondarily tested for genotyping. A total of 14 samples (4 originating from IRED and 10 from NRC-R) were analyzed, among them 13 (93%) provided

<table><caption>**Table 4.** Detection of viral RNA with RT-qPCR on Anigen test strip used in field conditions (IRED), in laboratory conditions (NRC-R) or combined.</caption><thead><tr><th rowspan="3"></th><th rowspan="3"></th><th colspan="9">Detection of viral RNA by RT-qPCR on Anigen test strip performed</th></tr><tr><th colspan="3">At IRED</th><th colspan="3">At NRC-R</th><th colspan="3">When combined</th></tr><tr><th>Pos</th><th>Neg</th><th>Total</th><th>Pos</th><th>Neg</th><th>Total</th><th>Pos</th><th>Neg</th><th>Total</th></tr></thead><tbody><tr><td rowspan="3">RIDT results</td><td>Pos</td><td>26</td><td>6</td><td>32</td><td>18</td><td>1</td><td>19</td><td>44</td><td>7</td><td>51</td></tr><tr><td>Neg</td><td>0</td><td>3</td><td>3</td><td>0</td><td>0</td><td>0</td><td>0</td><td>3</td><td>3</td></tr><tr><td>Total</td><td>26</td><td>9</td><td>35</td><td>18</td><td>1</td><td>19</td><td>44</td><td>10</td><td>54</td></tr></tbody></table>
<table><caption>Table 5. Comparison results of the detection of viral RNA with RT-qPCR performed on FTA Whatman cards and Anigen test strips.</caption><thead><tr><th rowspan="2"></th><th rowspan="2"></th><th colspan="3">Anigen test strip</th></tr><tr><th>Pos</th><th>Neg</th><th>Total</th></tr></thead><tbody><tr><td rowspan="3">FTA Whatman card</td><td>Pos</td><td>25</td><td>6</td><td>31</td></tr><tr><td>Neg</td><td>0</td><td>2</td><td>2</td></tr><tr><td>Total</td><td>25</td><td>8</td><td>33</td></tr></tbody></table>

doi:10.1371/journal.pntd.0005010.t005

PCR amplicons (at least 500 nucleotides in length) targeting regions of the nucleoprotein gene commonly used for genotyping after sequencing (S3 and S4 Tables). The only sample found negative (isolate 9702IND) was weakly positive after FAT and RIDT tests, which could then probably explain the absence of PCR amplification.

## Evaluation of the RIDT Anigen test in an inter-laboratory trial

Finally, we evaluated the RIDT Anigen test in an inter-laboratory trial, in parallel of the FAT on nine anonymous samples. The results obtained were concordant with those expected (Table 6) [28]. In particular, we were able to detect three different RABV isolates (strains CVS27 14–10, GS7 and DR627) constituting the panel, as well as 4 other lyssavirus species, including Duvenhage virus (DUVV), European bat lyssavirus 1 (EBLV-1) and 2 (EBLV-2), and Bokeloh bat lyssavirus (BBLV).

## Discussion

The aim of our study was to evaluate both in the laboratory and under field conditions the RIDT Anigen test in comparison with the FAT, to investigate the intrinsic parameters of this rapid technique, as well as the relevance of its application in sub-Saharan Africa, a region with the highest estimated per capita death rate due to rabies [4] but with poor data reporting situation [29].

We first investigated the limit of detection of this RIDT using serial dilutions of different titrated suspensions of RABV. This value varied among the isolates tested but remaining relatively high, ranked from 10⁵ to 10⁷ FFU.

We then evaluated the use of RIDT for post-mortem diagnosis of animal rabies in the laboratory and under field conditions, and compared it to the FAT assay. Our results demonstrate

<table><caption>Table 6. Evaluation of the RIDT Anigen test in NRC-R in an inter-laboratory trial.</caption><thead><tr><th>Sample</th><th>Lyssavirus species<sup>a</sup></th><th>Strain</th><th>FAT</th><th>RIDT</th></tr></thead><tbody><tr><td>1</td><td>RABV</td><td>CVS 27 14–10</td><td>Pos</td><td>Pos</td></tr><tr><td>2</td><td>RABV</td><td>GS7</td><td>Pos</td><td>Pos</td></tr><tr><td>3</td><td>RABV</td><td>DR627</td><td>Pos</td><td>Pos</td></tr><tr><td>4</td><td>BBLV</td><td>127900</td><td>Pos</td><td>Pos</td></tr><tr><td>5</td><td>DUVV</td><td>DUVV 05–11</td><td>Pos</td><td>Pos</td></tr><tr><td>6</td><td>EBLV-1</td><td>122938</td><td>Pos</td><td>Pos</td></tr><tr><td rowspan="2">7</td><td rowspan="2">EBLV-2</td><td>EBL2 RV1787</td><td rowspan="2">Pos</td><td rowspan="2">Pos</td></tr><tr><td>(1/30)</td></tr><tr><td>8</td><td>Negative</td><td>-</td><td>Neg</td><td>Neg</td></tr><tr><td>9</td><td>Negative</td><td>-</td><td>Neg</td><td>Neg</td></tr></tbody></table>

doi:10.1371/journal.pntd.0005010.t006

<footnote><sup>a</sup> RABV: Rabies virus, BBLV: Bokeloh bat lyssavirus, DUVV: Duvenhage virus, EBLV-1: European bat lyssavirus, EBLV-2: European bat lyssavirus 2.</footnote>
that the lateral flow test performs similar to the FAT. The accordance between RIDT and FAT was high under both conditions (≥94%), with a specificity of the RIDT from 93.3% to 100%. The sensitivity of this technique was also high in laboratory settings, with 95.3%, and approached 100% under field conditions. Our results indicate the high potential of this test for use in the resource challenged African context. Importantly, we show that the intrinsic performance of RIDT under limited laboratory conditions could be higher compared to the FAT test. Conversely to RIDT, several factors could affect negatively FAT results, including storage and quality of the fluorescent conjugate, maintenance of the fluorescence microscope and experience of the reader. However, given the limited sample size, the explanatory power is not overly strong and further evaluation is highly encouraged.

Lastly, we tested the RIDT Anigen test in parallel of the FAT technique in an international inter-laboratory trial organized by the European Union reference laboratory for rabies, Nancy, France and all results were found concordant.

As underlined by the results obtained under field conditions, the advantages of the immuno-chromatographic test method are manifold. Samples can be analysed one by one proportionate to the diagnostic demand. This is also true for FAT, however, the conjugate used for the FAT can only be stored for a limited time to ensure the quality of the test. Similarly, storage for the reagents is a cause for quality concern for the DRIT. For both DRIT and FAT, negative and positive controls have to be included in the test procedure for standardization, which is not needed for the RIDT [19]. Storage of the RIDT can be done at room temperature and does not require refrigeration, as is necessary for the conjugates used for the DRIT and FAT test. The tests used at IRED were stored at 20°C in an air conditioned room. There are no data on the reliability of the tests stored at temperatures at above 30°C, as would be encountered in many tropical countries.

The FAT depends heavily on the quality of the immunofluorescent conjugate, the maintenance of the fluorescent microscope and also on an experienced technician reading the microscope slides. In contrast, RIDT is a very easy-to-use kit, which does not require a high level of expertise. This technique is simple to perform and to interpret.

For the dog samples tested in Chad, the test had a specificity and sensitivity of 100%. However, we showed the utility of the Anigen test for many different wild and domestic mammalian species. Our results confirm data obtained from previous studies, and suggest that the spectrum of species which could be tested with this RIDT is larger than recommended by the manufacturer (e.g., dogs, cattle and raccoon dogs) [19, 20, 21].

In our study, we mainly focused on RABV species. In particular, we were able to detect RABV isolates belonging to all of the major phylogenetic clades defined previously [30], with the exception of the Africa 3 clade, which was not been tested in our panel. However, positive detection of isolates belonging to this clade has been already demonstrated [20]. In addition, we were also able to detect 4 different other lyssavirus species, in addition to RABV, when evaluated this technique in an inter-laboratory trial. These results are concordant with those obtained in two other previous studies using different lyssavirus species including African and bat-related lyssaviruses (including Lagos bat virus, Mokola virus and Australian bat lyssavirus), demonstrating that it can also be applicable for the detection of non-RABV lyssaviruses [19, 20, 31].

Several RIDT kits for RABV detection were evaluated using brain samples [18, 20, 21, 32, 33]. A very recent study evaluated 6 commercially available RIDT kits in parallel [34]. Sensitivity of the Anigen test in that study was observed to be unsatisfactory [34].

We modified the test procedure by omitting a dilution step and placing the brain sample directly into the buffer vial provided by the test. The advantage of this approach is that the test can be used with no additional material other than that provided in the kit. This change in
methodology might explain the better sensitivity of the test and the higher intensity of the test band compared to other studies [19, 22, 35], because the RABV antigen level is higher without a second dilution. Sharma et al. [35] found that the intensity of the test band decreases with dilution.

Autolysis of samples is less a concern for sensitivity of the RIDT compared to the FAT and PCR [17, 19, 36], which is illustrated by our results. Sample storage in glycerol was suggested to interfere with the optimal test performance, affecting the intensity of the test line [19].

The successful detection of RABV RNA from the Anigen test strip in over 86.3% of samples tested is a promising result and highlights the potential use of the kit as a vehicle for sample submission for further confirmatory diagnostic or genotyping analysis. This potential has also been reported by others [34]. However, the sensitivity was lower when compared to the use of the FTA Whatman card, a dedicated support for storage and preservation of nucleic acids.

The price of less than 10 euros is less expensive compared to the cost of performing FAT [19, 22], but still poses an affordability problem in developing countries.

Further validation has to be conducted with RIDT, especially if the results of this test will guide decision making for PEP. We demonstrated that the sensitivity of RIDT, even high, was not complete compared to FAT. To avoid getting false negative results with this technique, we suggest to confirm all negative results using WHO and OIE reference techniques, such as FAT, before excluding RABV infection in diagnostic samples.

An efficient diagnosis method is just part of the entire process of surveillance and control needed to eliminate rabies, as comparable to translation of efficiency of a vaccine, to the ultimate immunity of the target population [37]. Therefore, all components of the surveillance system, in which the test would be promoted and used, have to be strengthened in parallel [38].

## Conclusions

Specificity and sensitivity of the evaluated Anigen test are only slightly reduced compared to the known reference tests for rabies virus detection in brain samples. The results are promising for field use, where the test could help to establish rapid preliminary diagnostic results, which would be further confirmed using WHO and OIE recommended tests at central laboratories. However, we suggest important changes to the test protocol: skip the dilution step of brain biopsy in PBS and perform the brain homogenate with the swab directly into the specimen tube containing 1 ml of assay diluent, both provided in the kit. We also recommend to provide a more precise sketch depicting the brain sampling method. Rapid rabies tests cannot substitute for the current reference tests, but are crucial for the success of rabies surveillance systems in developing countries. Further, we demonstrated here that the test cassettes can be used as a vehicle to ship viral RNA to reference laboratories for further laboratory confirmation of the diagnosis and for epidemiological investigations.

## Supporting Information

S1 Table. Description of samples tested at NRC-R, Paris, France.
(DOCX)

S2 Table. Oligonucleotide sequences of primers and probes used in the combo RT-qPCR (combination of pan-RABV and pan-lyssa RT-qPCR assays) and in the internal control eGFP-based RT-qPCR assay.
(DOCX)

S3 Table. Comparison results obtained with samples from NRC-R for the *post-mortem* diagnosis of rabies using FAT and RIDT, and for the detection of rabies virus RNA using
**Anigen test strip as support material.**

(DOCX)

S4 Table. Comparison results obtained with samples from IRED for the *post-mortem* diagnosis of rabies using FAT and RIDT, and for the detection of rabies virus RNA using Anigen test strip and FTA Whatman card as support material.

(DOCX)

**Acknowledgments**

We are grateful to Thomas Müller and Conrad Freuling for helpful discussions and exchange of experience on the RIDT test. We thank Arovet Swiss for providing us the Anigen Rapid Rabies Test at a favourable rate for research purposes. We are also grateful to the three anonymous reviewers for their remarks which improved the manuscript.

**Author Contributions**

Conceived and designed the experiments: JZ LD HB IOA.

Performed the experiments: ML KN AL LD.

Analyzed the data: ML LD.

Contributed reagents/materials/analysis tools: IOA.

Wrote the paper: ML HB JZ LD.

**References**

1. Jackson AC. Current and future approaches to the therapy of human rabies. Antiviral Res. 2013; 99 (1):61-7. doi: 10.1016/j.antiviral.2013.01.003 PMID: 23369672

2. Fooks AR, Banyard AC, Horton DL, Johnson N, McElhinney LM, Jackson AC. Current status of rabies and prospects for elimination. Lancet. 2014. Oct 11; 384(9951):1389-99. doi: 10.1016/S0140-6736 (13)62707-5 PMID: 24828901

3. Knobel DL, Cleaveland S, Coleman PG, Fevre EM, Meltzer MI, Miranda ME, et al. Re-evaluating the burden of rabies in Africa and Asia. Bull World Health Organ. 2005; 83(5):360-8. PMID: 15976877

4. Hampson K, Coudeville L, Lembo T, Sambo M, Kieffer A, Attlan M, et al. Estimating the global burden of endemic canine rabies. PLoS Negl Trop Dis. 2015; 9(4):e0003709. doi: 10.1371/journal.pntd.0003709 PMID: 25881058

5. Anderson A, Shwiff SA. The Cost of Canine Rabies on Four Continents. Transbound Emerg Dis. 2015 Aug; 62(4):446-52. doi: 10.1111/tbed.12168 PMID: 24112194

6. WHO Expert Consultation on Rabies. Second report. World Health Organ Tech Rep Ser. 2013 (982):1-139.

7. Nel LH. Discrepancies in data reporting for rabies, Africa. Emerg Infect Dis. 2013; 19(4):529-33. doi: 10.3201/eid1904.120185 PMID: 23628197

8. Lembo T, Attlan M, Bourhy H, Cleaveland S, Costa P, de Balogh K, et al. Renewed global partnerships and redesigned roadmaps for rabies prevention and control. Vet Med Int. 2011; 2011:923149. doi: 10.4061/2011/923149 PMID: 21776359

9. Mallewa M, Fooks AR, Banda D, Chikungwa P, Mankhambo L, Molyneux E, et al. Rabies encephalitis in malaria-endemic area, Malawi, Africa. Emerg Infect Dis. 2007; 13(1):136-9. PMID: 17370529

10. Klepac P, Metcalf CJE, McLean AR, Hampson K. Towards the endgame and beyond: complexities and challenges for the elimination of infectious diseases. Philos Trans R Soc Lond B Biol Sci. 2013; 368(1623).

11. Townsend SE, Lembo T, Cleaveland S, Meslin FX, Miranda ME, Putra AAG, et al. Surveillance guidelines for disease elimination: A case study of canine rabies. Comp Immunol Microbiol Infect Dis. 2013; 36(3):249-61. doi: 10.1016/j.cimid.2012.10.008 PMID: 23260376
12. Dean D, Abelseth M, Atanasiu P. The fluorescent antibody test. Laboratory techniques in rabies. 1996; 4:88-95.

13. Bourhy H, Rollin PE, Vincent J, Sureau P. Comparative field evaluation of the fluorescent-antibody test, virus isolation from tissue culture, and enzyme immunodiagnosis for rapid laboratory diagnosis of rabies. J Clin Microbiol. 1989; 27(3):519-23. PMID: 2654181

14. Dürr S, Mindekem R, Diguimbye C, Niezgoda M, Kuzmin I, Rupprecht CE, et al. Rabies diagnosis for developing countries. PLoS Negl Trop Dis. 2008; 2(3):e206. doi: 10.1371/journal.pntd.0000206 PMID: 18365035

15. Dyer JL, Niezgoda M, Orciari LA, Yager PA, Ellison JA, Rupprecht CE. Evaluation of an indirect rapid immunohistochemistry test for the differentiation of rabies virus variants. J Virol Methods. 2013; 190 (1):29-33.

16. Sambo M, Lembo T, Cleaveland S, Ferguson HM, Sikana L, Simon C, et al. Knowledge, attitudes and practices (KAP) about rabies prevention and control: a community survey in Tanzania. PLoS Negl Trop Dis. 2014; 8(12):e3310. doi: 10.1371/journal.pntd.0003310 PMID: 25473834

17. McElhinney LM, Marston DA, Brookes SM, Fooks AR. Effects of carcase decomposition on rabies virus infectivity and detection. J Virol Methods. 2014; 207:110-3. doi: 10.1016/j.jviromet.2014.06.024 PMID: 25010791

18. Kang B, Oh J, Lee C, Park BK, Park Y, Hong K, et al. Evaluation of a rapid immunodiagnostic test kit for rabies virus. J Virol Methods. 2007; 145(1):30-6. PMID: 17628707

19. Servat A, Picard-Meyer E, Robardet E, Muzniece Z, Must K, Cliquet F. Evaluation of a Rapid Immunochromatographic Diagnostic Test for the detection of rabies from brain material of European mammals. Biologicals. 2012; 40(1):61-6. doi: 10.1016/j.biologicals.2011.12.011 PMID: 22245544

20. Markotter W, York D, Sabeta CT, Shumba W, Zulu G, Le Roux K et al. Evaluation of a rapid immunodiagnostic test kit for detection of African lyssaviruses from brain material. Onderstepoort J Vet Res. 2009 Jun; 76(2):257-62 PMID: 20698445

21. Reta T, Teshale S, Deresa A, Ali A, Getahun G. Evaluation of rapid immunodiagnostic test for rabies diagnosis using clinical brain samples in ethiopia. J Vet Sci Med Diagn. 2013; 2:2.

22. Voehl KM, Saturday GA. Evaluation of a rapid immunodiagnostic rabies field surveillance test on samples collected from military operations in Africa, Europe, and the Middle East. US Army Med Dep J. 2014:27-32.

23. Anonymous. Anigen Rapid Rabies AG test kit. BioNote. 2010, 1 Available: http://www.bionote.co.kr/File/Upload/2011/02/16/2011-02-16%285%29.pdf

24. Picard-Meyer E, Barrat J, Cliquet F. Use of filter paper (FTA) technology for sampling, recovery and molecular characterisation of rabies viruses. J Virol Methods. 2007; 140(1):174-82.

25. Dacheux L, Reynes JM, Buchy P, Sivuth O, Diop BM, Rousset D, et al. A reliable diagnosis of human rabies based on analysis of skin biopsy specimens. Clin Infect Dis. 2008; 47(11):1410-7. doi: 10.1086/592969 PMID: 18937576

26. Dacheux L, Larrous F, Lavenir R, Lepelletier A, Faouzi A, Troupin C, Nourlil J, Buchy P, Bourhy H. Dual Combined Real-Time Reverse Transcription Polymerase Chain Reaction Assay for the Diagnosis of Lyssavirus Infection. PLoS Negl Trop Dis. 2016 Jul 5; 10(7):e0004812. doi: 10.1371/journal.pntd.0004812 PMID: 27380028

27. Bourhy H, Nakouné E, Hall M, Nouvellet P, Lepelletier A, Talbi C, Watier L, Holmes EC, Cauchemez S, Lemey P, Donnelly CA, Rambaut A. Revealing the Micro-scale Signature of Endemic Zoonotic Disease Transmission in an African Urban Setting. PLoS Pathog. 2016 Apr 8; 12(4):e1005525. doi: 10.1371/journal.ppat.1005525 PMID: 27058957

28. Robardet E, Picard-Meyer E, Andrieu S, Servat A, Cliquet F. International interlaboratory trials on rabies diagnosis: an overview of results and variation in reference diagnosis techniques (fluorescent antibody test, rabies tissue culture infection test, mouse inoculation test) and molecular biology techniques. J Virol Methods. 2011 Oct; 177(1):15-25. doi: 10.1016/j.jviromet.2011.06.004 PMID: 21703307

29. Taylor LH, Knopf L, the Partners for Rabies P. Surveillance of Human Rabies by National Authorities —A Global Survey. Zoonoses Public Health. 2015 Nov; 62(7):543-52. doi: 10.1111/zph.12183 PMID: 25683444

30. Bourhy H, Reynes JM, Dunham EJ, Dacheux L, Larrous F, Huong VT, Xu G, Yan J, Miranda ME, Holmes EC. The origin and phylogeography of dog rabies virus. J Gen Virol. 2008 Nov; 89 (Pt 11):2673-81 doi: 10.1099/vir.0.2008/003913-0 PMID: 18931062

31. Mshelbwala P, Abdullahi S, Maikai B, Onyiche E, Ogunkoya A. Evaluation of Two Rapid Diagnostic Tests for Rabies Diagnosis under Field and Laboratory Conditions in Nigeria. J Vaccines Vaccin. 2015; 6(272):2.
32. Nishizono A, Khawplod P, Ahmed K, Goto K, Shiota S, Mifune K, et al. A simple and rapid immunochromatographic test kit for rabies diagnosis. Microbiol Immunol. 2008; 52(4):243-9. doi: 10.1111/j.1348-0421.2008.00031.x PMID: 18426399

33. Ahmed K, Wimalaratne O, Dahal N, Khawplod P, Nanayakkara S, Rinzin K, et al. Evaluation of a Monoclonal Antibody-Based Rapid Immunochromatographic Test for Direct Detection of Rabies Virus in the Brain of Humans and Animals. Am J Trop Med Hyg. 2012; 86(4):736-40. doi: 10.4269/ajtmh.2012.11-0332 PMID: 22492163

34. Eggerbauer E, DeBenedictis P, Hoffmann B, Mettenleiter T.C, Schlottau K, Sabeta C.T, Freuling C.M and Müller T. Evaluation of six commercially available rapid immunochromatographic tests for the diagnosis of rabies in brain material. PLoS Negl Trop Dis 2016 Jun 23; 10(6):e0004776. doi: 10.1371/journal.pntd.0004776 PMID: 27336943

35. Sharma P, Singh C, Narang D. Comparison of immunochromatographic diagnostic test with hemi-nested reverse transcriptase polymerase chain reaction for detection of rabies virus from brain sam-ples of various species. Vet World. 2015; 8(2):135-8. doi: 10.14202/vetworld.2015.135-138 PMID: 27047061

36. Silva S, Katz I, Mori E, Carnieli P, Vieira L, Batista H, et al. Biotechnology advances: a perspective on the diagnosis and research of Rabies Virus. Biologicals. 2013; 41(4):217-23. doi: 10.1016/j.biologicals.2013.04.002 PMID: 23683880

37. Muthiani Y, Traore A, Mauti S, Zinsstag J, Hattendorf J. Low coverage of central point vaccination against dog rabies in Bamako, Mali. Prev Vet Med. 2015; 120(2):203-9. doi: 10.1016/j.prevetmed.2015.04.007 PMID: 25953653

38. Lembo T, Partners for Rabies P. The blueprint for rabies prevention and control: a novel operational toolkit for rabies elimination. PLoS Negl Trop Dis. 2012; 6(2):e1388. doi: 10.1371/journal.pntd.0001388 PMID: 22389727