# Structural basis for galectin-1-dependent pre-B cell receptor (pre-BCR) activation.

Latifa El Antak, Marion Espeli, Annie Boned, Olivier Bornet, Jeremy Bonzi, Laurent Gauthier, Mikael Feracci, Philippe Roche, Françoise Guerlesquin, Claudine Schiff

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## To cite this version:

Latifa El Antak, Marion Espeli, Annie Boned, Olivier Bornet, Jeremy Bonzi, et al. Structural basis for galectin-1-dependent pre-B cell receptor (pre-BCR) activation. Journal of Biological Chemistry, 2012, 287 (53), pp.44703-13. 10.1074/jbc.M112.395152. hal-01458259

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Submitted on 27 May 2021

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# Structural Basis for Galectin-1-dependent Pre-B Cell Receptor (Pre-BCR) Activation*

Received for publication, July 3, 2012, and in revised form, October 24, 2012 Published, JBC Papers in Press, November 2, 2012, DOI 10.1074/jbc.M112.395152

Latifa Elantak†, Marion Espeli§<fnref n="1" />, Annie Boned§, Olivier Bornet¶, Jeremy Bonzi†‡, Laurent Gauthier||, Mikael Feracci**, Philippe Roche†‡, Françoise Guerlesquin‡±, and Claudine Schiff§<fnref n="3" />

From the ‡Laboratoire d'Ingénierie des Systèmes Macromoléculaires, CNRS UMR7255, Aix-Marseille Université, 13402 Marseille cedex 20, France, the §Centre d'Immunologie de Marseille-Luminy, Aix-Marseille Université, UM2, INSERM U1104, CNRS UMR7280, 13288 Marseille cedex 09, France, the ¶Institut de Microbiologie de la Méditerranée, FR 3479, 13402 Marseille cedex 20, France, ||Innate Pharma, 13276 Marseille, France, the **Department of Biochemistry, University of Leicester, LE1 9HN Leicester, United Kingdom, and the ‡‡Laboratory of Integrative Structural and Chemical Biology, INSERM U1068, CNRS UMR7258, Aix-Marseille Université, Institut Paoli Calmettes, 13273 Marseille cedex 09, France

**Background:** Galectin-1 (GAL1) is a ligand for the pre-BCR which is involved in the proliferation and differentiation of normal pre-BII cells.

**Results:** GAL1-dependent pre-BCR clustering is driven mainly by hydrophobic contacts.

**Conclusion:** Constitutive and ligand-induced pre-BCR activation can occur in a complementary manner.

**Significance:** This is the first molecular snapshot of a pre-BCR/ligand interaction that helps pre-BCR clustering and activation.

During B cell differentiation in the bone marrow, the expression and activation of the pre-B cell receptor (pre-BCR) constitute crucial checkpoints for B cell development. Both constitutive and ligand-dependent pre-BCR activation modes have been described. The pre-BCR constitutes an immunoglobulin heavy chain (Igμ) and a surrogate light chain composed of the invariant λ5 and VpreB proteins. We previously showed that galectin-1 (GAL1), produced by bone marrow stromal cells, is a pre-BCR ligand that induces receptor clustering, leading to efficient pre-BII cell proliferation and differentiation. GAL1 interacts with the pre-BCR via the unique region of λ5 (λ5-UR). Here, we investigated the solution structure of a minimal λ5-UR motif that interacts with GAL1. This motif adopts a stable helical conformation that docks onto a GAL1 hydrophobic surface adjacent to its carbohydrate binding site. We identified key hydrophobic residues from the λ5-UR as crucial for the interaction with GAL1 and for pre-BCR clustering. These residues involved in GAL1-induced pre-BCR activation are different from those essential for autonomous receptor activation. Overall, our

results indicate that constitutive and ligand-induced pre-BCR activation could occur in a complementary manner.

B cell differentiation in the bone marrow is a highly regulated process. At the pre-BII cell stage, newly generated IgH chains are probed for their ability to interact with surrogate light chains (SLC)<fnref n="4" /> and form a pre-B cell receptor (pre-BCR) that will be expressed at the cell surface (1). The expression of the pre-BCR by pre-BII cells constitutes a critical checkpoint because it controls pre-BII cell proliferation and differentiation (2) and mediates the selection of the Igμ chain repertoire (3), leading to the counterselection of autoreactive Igμ chains (4). In mice and humans, mutations in the genes encoding this receptor result in immunodeficiency, leukemia, and autoimmunity.

The pre-BCR is composed of two Igμ chains, two SLC and the CD79a/b signaling molecules. The crystal structure of the SLC, composed of the λ5 and VpreB invariant proteins, exhibits a structure similar to the IgL chain (5). The individuality of λ5 and VpreB is contained within their N and C terminus regions, respectively, and they are termed unique regions (URs) because they share no sequence similarity to known proteins. Whereas a portion of the structure of the VpreB-UR 24 amino acids has been solved, no structural data are available for the 52 amino acids forming the λ5-UR (5). Several functional studies have shown that the λ5-UR is essential for pre-BCR activation and function (6-9).

It was first thought that surface expression and co-aggregation of the pre-BCR at the cell surface were sufficient to induce pre-BII cell proliferation and differentiation toward immature B cells (9, 10). However, we reported the formation of an

<footnote>* This work was supported by grants from the Agence Nationale de la Recherche (ANR-05-BLAN-0035-01), ARC (to M. E.), INSERM, and CNRS and the Fondation pour la Recherche Médicale (to L. E.), the WeNMR Project European FP7 e-Infrastructure grant, Contract 261572, for the use of Web portals, computing, and storage facilities.</footnote>

<footnote>§ This article contains supplemental Tables S1 and S2, Figs. S1-S7, and additional references.</footnote>

<footnote>The NMR chemical shifts have been deposited in the BioMagResBank, www.bmrb.wisc.edu (accession no. 18009).</footnote>

<footnote>The atomic coordinates and structure factors (code 2KLQ) have been deposited in the Protein Data Bank (http://wwpdb.org/).</footnote>

<footnote n="1">¹ Present address: University of Cambridge School of Clinical Medicine, Cambridge CB2 2OY, United Kingdom.</footnote>

<footnote n="2">² To whom correspondence may be addressed: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, CNRS UMR7255, Aix-Marseille Université, 31 chemin Joseph Aiguier, 13402 Marseille cedex 20, France. Tel.: 33-491-164-379; Fax: 33-491-164-540; E-mail: guerlesquin@imm.cnrs.fr.</footnote>

<footnote n="3">³ To whom correspondence may be addressed: Centre d'Immunologie de Marseille-Luminy, Aix-Marseille Université, UM2, INSERM U1104, CNRS UMR7280, case 906, 13288 Marseille cedex 09, France. Tel.: 33-491-269-448; Fax: 33-491-269-430; E-mail: schiff@ciml.univ-mrs.fr.</footnote>

<footnote n="4">⁴ The abbreviations used are: SLC, surrogate light chain; CBS, carbohydrate binding site; CRD, carbohydrate recognition domain; GAL1, galectin-1; HSQC, heteronuclear single quantum coherence; PDB, Protein Database; pre-BCR, pre-B cell receptor; TOCSY, total correlated spectroscopy; UR, unique region.</footnote>
## Galectin-1/Pre-BCR Interaction

immune developmental synapse between stromal and pre-BII cells where the pre-BCRs relocalize at the cell contact zone, leading to pre-BCR activation and the proliferation and differentiation of pre-BII cells (7, 8, 11). In humans and mice, the formation of the immune synapse takes place through a network of interactions involving galectin-1 (GAL1). During synapse formation, GAL1 binds to the λ5-UR through a protein/ protein interaction and to glycosylated integrins on stromal and pre-BII cells via protein/carbohydrate interactions (8, 11). Consistent with the involvement of GAL1 in pre-BCR relocalization and signaling, GAL1-deficient mice have a defect in pre-BII cell differentiation and proliferation (7). Moreover, GAL1-expressing stromal cells constitute a specific cellular niche for normal pre-BII cells in the bone marrow (12).

GAL1 is a member of the galectin family, which is composed of 15 structurally related proteins with an affinity for β-galactosides (13). They are defined as a shared consensus of amino acid sequence and by their carbohydrate recognition domain (CRD) responsible for β-galactoside binding (14). The carbohydrate binding site (CBS) delineates the CRD pocket where carbohydrates interact. All CRDs possess a remarkably similar fold that is composed of two antiparallel β-sheets of five and six β-strands, arranged in a β-sheet sandwich motif with a jelly roll topology (13–15). GAL1 is implicated in a wide range of biological activities including cell cycle regulation, adhesion, proliferation, and apoptosis (13). Most of the structural studies have focused on GAL1/β-galactoside interaction, but GAL1 protein partners have also been identified (13). These proteins interact in a carbohydrate-independent manner with GAL1, but no detailed structural data on these interactions have been reported yet.

Given the major impact of GAL1 on pre-BII cell development, it is essential to understand the molecular basis for the GAL1/pre-BCR interaction and to know how pre-BCR relocalization is influenced by this interaction. We now report NMR investigations that reveal the helical structure of the λ5-UR central region and the structural basis of GAL1/λ5-UR interactions that are essential for synapse formation between pre-B and stromal cells.

## EXPERIMENTAL PROCEDURES

*Cloning, Expression, and Purification of GAL1 Proteins and λ5-UR Domains; λ5-UR<sup>22-45</sup> Peptide Production*—The human GAL1 gene sequence was cloned as described previously (11). The oligonucleotides used for this study are listed in supplemental Table S1. The GAL1 mutants were generated using the QuikChange® Site-directed Mutagenesis kit (Stratagene). The production and purification of the GAL1 proteins were performed as described previously (16). For the production of <sup>15</sup>N- or <sup>15</sup>N- and <sup>13</sup>C-labeled proteins, the cells were grown in M9 minimal medium supplemented with 1 g/liter <sup>15</sup>NH<sub>4</sub>Cl and 2 g/liter D-[<sup>13</sup>C]glucose when needed as the sole nitrogen and carbon sources, respectively. The protein production was induced using 1 mM isopropyl-β-D-thiogalactopyranoside when the A<sub>600</sub> of the cell culture reached 0.7, and the cells were incubated at 37 °C for 3 h and were collected by centrifugation. The unlabeled proteins were produced using the same method, except that LB was used as the culture medium.

For the NMR studies, the gene encoding the human λ5-UR domain was cloned into a modified pET28a vector (Novagen), which contained an N-terminal His<sub>6</sub> tag fused to a lipoyl domain (17), followed by a tobacco etch virus cleavage site. For the purification of the λ5-UR, the cells were disrupted using a French press, and the resulting supernatant was clarified by centrifugation at 125,000 × g. The clarified supernatant was applied to a 5-ml HisTrap<sup>TM</sup> HP column (GE Healthcare) in accordance with the manufacturer's instructions. After elution, the lipoyl domain was removed from the λ5-UR by tobacco etch virus protease digestion. The digestion mixture was applied to a HisTrap<sup>TM</sup> HP column. The flow-through containing the cleaved λ5-UR was concentrated in an Amicon (Millipore) concentrator, and the samples were applied to a Superdex<sup>TM</sup> 75 (10/300GL) gel filtration column (GE Healthcare). The buffer used in the gel filtration experiments was 20 mM sodium phosphate (pH 5.2) (NMR buffer). The chromatographic purifications were performed using the ÄKTA PrimePlus purification system (GE Healthcare).

For the pre-BCR relocalization assays, the gene sequences of the WT and mutant λ5-UR were cloned as described previously (7) to generate proteins fused to a GST tag. The production and purification of the human His-λ5-UR-GST were described previously (7). The recombinant proteins were expressed in the BL21-RP strain (Stratagene). The cells were disrupted using a French press, and the resulting supernatant was clarified by centrifugation at 125,000 × g. The clarified supernatant was purified on glutathione-Sepharose 4B (GE Healthcare) in accordance with the manufacturer's instructions.

The purity and integrity of all proteins were analyzed by SDS-PAGE. The λ5-UR<sup>22-45</sup>, λ5-UR<sup>22-45</sup>-R27A/R29A, and λ5-UR<sup>22-45</sup>-L26A/W30A peptides were chemically synthesized and purchased (Schafer-N, Copenhagen, Denmark).

*NMR Experiments*—The NMR experiments were performed at 303 K on a Bruker Avance 800 spectrometer equipped with a TXI cryoprobe, or on a Bruker Avance 600 spectrometer equipped with a TCI cryoprobe. The NMR samples contained proteins in 20 mM phosphate buffer (pH 5.2).

The NMR titration was performed using two-dimensional <sup>1</sup>H,<sup>15</sup>N HSQC NMR spectra recorded on <sup>15</sup>N-labeled GAL1 at 0.7 mM concentration (monomer concentration) in the absence and in the presence of increasing amount of λ5-UR<sup>22-45</sup> peptide (0.175, 0.35, 0.525, 0.7, 1 mM). The sample buffer contained 20 mM KPO<sub>4</sub> (pH 5.2), 100 mM NaCl, 1.4 mM lactose, 10% D<sub>2</sub>O. The chemical shift perturbations for each resonance were calculated using the equation

$$ \Delta\delta_{\text{obs}} = ((\Delta\delta_{\text{HN}}^2 + \Delta\delta_{\text{N}}^2/25))^{1/2} \quad (\text{Eq. 1}) $$

where Δδ<sub>HN</sub> and Δδ<sub>N</sub> are, respectively, the proton and nitrogen chemical shifts variation of each residue (18). The dissociation constant (K<sub>D</sub>) of GAL1/λ5-UR<sup>22-45</sup> complex formation was obtained by plotting the chemical shift changes from four selected amide cross-peaks (belonging to residues Arg-73, Glu-74, Asn-102, and Phe-106) against the total concentration of the λ5-UR<sup>22-45</sup> peptide. Titration curves were fitted with a sigmoidal function, and a K<sub>D</sub> was determined for each curves. The final K<sub>D</sub> value is the average of the four obtained values.

<figure><img src="image_3.png" /></figure>
The lactose binding activity of GAL1 has been determined by recording ¹H,¹⁵N HSQC spectra of 0.2 mM ¹⁵N-labeled GAL1 after addition of increasing amount of lactose (0.05, 0.1, 0.15, 0.2, 0.25, 0.3, 0.4, 0.8, 2, and 8 mM). These experiments have been performed either in the absence or in the presence of 3 molar equivalent of λ5-UR²²⁻⁴⁵ (0.6 mM). Chemical shift variations of His-52 and Trp-68 were calculated using the same equation as above, and K_D values were extracted from the fit of the titration curves.

Effects of λ5-UR mutations on the complex formation were obtained by NMR chemical shift perturbation mapping on ¹⁵N-GAL1 HSQC spectra in the absence and the presence of λ5-UR²²⁻⁴⁵ mutated peptides. Effects of GAL1 mutations on the complex formation were obtained by NMR chemical shift perturbation mapping performed on mutated ¹⁵N-GAL1 proteins in the absence and the presence of λ5-UR²²⁻⁴⁵.

The two-dimensional ¹H TOCSY and two-dimensional ¹H NOESY experiments were performed on free λ5-UR²²⁻⁴⁵ for resonance assignment and structure calculation. The resonance assignments and coordinates of the λ5-UR²²⁻⁴⁵ domain structure have been deposited in the BioMagResBank (accession no. 18009) and the Protein Database (PDB ID code 2LKQ), respectively. The two-dimensional ¹⁵N,¹³C-filtered NOESY spectra were recorded on the λ5-UR²²⁻⁴⁵/¹⁵N,¹³C-GAL1 sample at a ratio of 1.25/1 for calculation of the bound λ5-UR²²⁻⁴⁵ structure. The backbone sequential resonances of ¹⁵N,¹³C-labeled GAL1 bound to λ5-UR²²⁻⁴⁵ were assigned using three-dimensional HNCA, HN(CO)CA, CBCA(CO)NH experiments, and side chain resonances were assigned using three-dimensional HCCH-TOCSY experiments. The intermolecular NOEs between the λ5-UR²²⁻⁴⁵ and ¹⁵N,¹³C-GAL1 were obtained using two-dimensional ¹⁵N,¹³C half-filtered NOESY experiments.

The structures of the free and bound λ5-UR²²⁻⁴⁵ peptides were calculated using the CYANA software. Next, these structures were water-refined in a minimization run using the SANDER module of AMBER 9.0 software (19). The quality of each structure was assessed using the PROCHECK-NMR program (20). The structural model of the complex was calculated using the HADDOCK program (21).

**Immunofluorescence Imaging**—The co-cultures of the Nalm6 pre-B cells and OP9 stromal cells were performed as described previously (7), in the presence or the absence of the indicated His-λ5-UR-GST proteins (40 µg/ml). After fixation, the cells were stained with goat anti-human IgM antibodies (Southern Biotech) and were visualized using AF555-labeled donkey anti-goat IgG (Invitrogen) or rabbit anti-GAL1 antiserum (8) and AF488-labeled goat anti-rabbit IgG (Invitrogen). The cells were mounted and analyzed on a LSM510 Carl Zeiss confocal microscope. The slides were scanned by differential interferential contrast imaging, and 100–380 pre-B cells in contact with stromal cells were examined further for their surface fluorescence distribution. The inhibition of pre-BCR relocalization was calculated as described previously (7). The S.D. values were calculated from three independent experiments. The *p* values were determined using the Mann-Whitney unpaired test with a risk of 5%, n.s = not significant; *, *p* ≤ 0.05; **, *p* ≤ 0.01; ***, *p* ≤ 0.001.

## RESULTS

*The λ5-UR²²⁻⁴⁵ Fragment Contains the Determinants Required for GAL1 Binding*—We have shown previously that the GAL1-interacting site on the pre-BCR is located within the λ5-UR domain (7, 8). The theoretical predictions (supplementary Fig. S1 and Ref. 22) indicate that the full-length λ5-UR (45 amino acids from Ser-8 to Ser-52) contains a potential short α-helix in its middle region (Ser-28 to Arg-37) whereas the rest of the domain is unfolded (supplemental Fig. S1C). In agreement, the λ5-UR CD spectrum (supplemental Fig. S1D) exhibits a weak shoulder in the 209–222-nm region corresponding to helical structures, and a strong negative band near 200 nm represents random coil conformations. This type of spectrum is typical of proteins having a low level of α-helical structures.

To investigate whether this potential small helical region of λ5-UR constitutes a sufficient binding epitope for GAL1, a 24-mer peptide covering amino acids 22–45 (λ5-UR²²⁻⁴⁵) was chemically synthesized and tested for binding to GAL1 using NMR spectroscopy. An NMR titration was performed by recording two-dimensional ¹H,¹⁵N HSQC spectra of the ¹⁵N-labeled GAL1 bound to lactose in the absence and the presence of increasing amount of the λ5-UR²²⁻⁴⁵ peptide (Fig. 1A). We monitored the perturbations in the NMR signal of the ¹⁵N-GAL1 homodimer (28 kDa), making use of the previously published resonance assignment of human GAL1 bound to lactose (23). Significant variations were observed implying that a specific interaction occurred. The binding was in the fast exchange regime on the NMR chemical shift time scale and indicated a 1:1 complex stoichiometry (one λ5-UR/GAL1 monomer). Chemical shift perturbations (Fig. 1B) of four GAL1 residues (Arg-73, Glu-74, Asp-102, Phe-106) chosen among the peaks experiencing the largest variations were plotted against λ5-UR²²⁻⁴⁵ concentration (Fig. 1C). The average K_D value derived from the fit of the curves corresponds to 310 ± 65 µM.

Upon λ5-UR²²⁻⁴⁵ binding, GAL1 residues experiencing significant chemical shift displacements are localized in the CBS (including strands β3, β4, and β5) and on a surface adjacent to this region (including strands β6, β7, and β9) (Fig. 1, D and E). The same regions of GAL1, localized at its side and back face, undergo chemical shift variations upon λ5-UR²²⁻⁴⁵ addition in the absence of lactose (Fig. 2, A and B, and supplemental Fig. S2A). These observations indicate that λ5-UR²²⁻⁴⁵ uses the same mode of binding to GAL1 in the absence and in the presence of lactose.

The same chemical shift perturbation mapping experiment was performed using ¹⁵N-labeled GAL1 and the full-length λ5-UR instead of the λ5-UR²²⁻⁴⁵ peptide (Fig. 2C and supplemental Fig. S2B). Once again, similar effects were observed with the same regions of GAL1 experiencing changes upon λ5-UR binding (compare Fig. 2, C with A). This result indicates that the λ5-UR²²⁻⁴⁵ fragment uses the same mode of binding to GAL1 as the full-length λ5-UR and therefore contains the sequence and structure determinants required for GAL1 binding.

*The λ5-UR²²⁻⁴⁵ Contains an α-Helix Structure in Solution and in Complex with GAL1*—To verify the presence of an α-helical structure within λ5-UR²²⁻⁴⁵, first we solved the solution structure of the free λ5-UR²²⁻⁴⁵ peptide using homo-
Galectin-1/Pre-BCR Interaction

nuclear NMR spectroscopy. Using the CYANA software and
based on 231 distance restraints among which 49 were medium
range (supplemental Fig. S3A), 100 structures were calculated,
and the 20 lowest energy structures are shown in Fig. 3A. The
structural statistics (supplemental Table S2) demonstrate a well

defined structure with low pairwise root mean square deviation
values of 1.3 ± 0.08 Å (0.89 ± 0.07 Å for residues 25–41). The
structure of the free λ5-UR²²⁻⁴⁵ peptide reveals the presence of
a short 10-amino acid α-helix (from residues Arg-29 to Ser-39)
among the 24 residues of the peptide (Fig. 3A), whereas the N-

<figure><img src="image_4.png" /><figcaption>B</figcaption></figure>

<figure><img src="image_5.png" /><figcaption>C</figcaption></figure>

<figure><img src="image_6.png" /><figcaption>D</figcaption></figure>

<figure><img src="image_7.png" /><figcaption>E</figcaption></figure>

<figure><img src="image_8.png" /></figure>

<figure><img src="image_9.png" /></figure>
<figure><img src="image_10.png" /><figcaption>FIGURE 2. λ5-UR<sup>22-45</sup> and λ5-UR interaction sites on GAL1. A, normalized chemical shift perturbations induced upon addition of 1 molar equivalent of λ5-UR<sup>22-45</sup> to <sup>15</sup>N-labeled GAL1 in the absence of lactose, as monitored in <sup>1</sup>H, <sup>15</sup>N HSQC spectra (supplemental Fig. S2A). The plot is color-coded according to the location of the residues showing the largest variations on GAL1 structure (orange, side and back face; pink, CBS). The secondary structures of GAL1 are shown above the plot. B, GAL1 chemical shift perturbations upon addition of λ5-UR<sup>22-45</sup> mapped onto the surface representation of the GAL1 dimer structure (PDB ID code 2KM2 (24)). The residues are labeled and color-coded as in A. A single GAL1 monomer is circled. C, normalized chemical shift changes induced upon addition of 1 molar equivalent of λ5-UR to <sup>15</sup>N-labeled GAL1 (0.1 mM), as monitored in <sup>1</sup>H, <sup>15</sup>N HSQC spectra (supplemental Fig. S2B). The plot is color-coded as in A.</figcaption></figure>

and C-terminal residues flanking the helix are not structured in agreement with the theoretical predictions (supplemental Fig. S1C) (6).

The structure of the λ5-UR<sup>22-45</sup> peptide bound to GAL1 was then investigated using a <sup>15</sup>N,<sup>13</sup>C-filtered NOESY spectrum

recorded on the λ5-UR<sup>22-45</sup>/<sup>15</sup>N,<sup>13</sup>C-labeled GAL1 sample. In this experiment, only the resonances belonging to the unlabeled protein (i.e. λ5-UR<sup>22-45</sup>) are detected. After proton resonances assignment, NOE-derived distance restraints were collected (supplemental Fig. S3B and supplemental Table S2). The structure calculations performed confirmed the existence of the helix when λ5-UR<sup>22-45</sup> is bound to GAL1 (Fig. 3B) as illustrated by the conservation of the helix-forming restraints (Fig. 3C). Thus, a short α-helix within the λ5-UR<sup>22-45</sup> preexists in solution and its structure is conserved upon binding to GAL1. We next investigated whether this helix plays a crucial role in GAL1 binding.

λ5-UR<sup>22-45</sup> Binds to GAL1 on a Surface Close to the CBS and Induces Changes in GAL1 Lactose Binding Activity—To gain further insight into the possible modes of interaction of the λ5-UR<sup>22-45</sup> peptide with GAL1, a structural model of the complex was calculated using the HADDOCK program (21). We used the 10 best NMR structures of the λ5-UR<sup>22-45</sup> peptide and the lowest energy NMR structure of the human GAL1 (24). On the basis of the λ5-UR<sup>22-45</sup> peptide and GAL1 resonance assignments that we performed on the complexed forms, 10 intermolecular NOE restraints have been assigned in the <sup>15</sup>N,<sup>13</sup>C half-filtered NOESY spectrum recorded on the <sup>15</sup>N,<sup>13</sup>C GAL1/λ5-UR<sup>22-45</sup> complex (supplemental Fig. S4). These intermolecular NOE restraints have been used to restrain the dockings. The GAL1/λ5-UR<sup>22-45</sup> complex was a good candidate for this structure calculation strategy because neither protein undergoes large scale conformational changes upon complex formation as seen by the limited extent of chemical shift perturbations (Fig. 1D). The docking calculations yielded a dominant cluster of 182 solutions of the 200 solutions obtained.

The structural model (Fig. 4A) shows that the λ5-UR<sup>22-45</sup> peptide binds to a surface localized at the interface between the two GAL1 β-sheets near the CBS (Fig. 4B). This surface corresponds to the area adjacent to the CBS highlighted by the chemical shift perturbation mapping (Fig. 1E, orange surface). The close proximity of the CBS and of the λ5-UR<sup>22-45</sup> interacting surface on GAL1 led us to investigate the lactose binding activity of GAL1 in the absence and in the presence of λ5-UR<sup>22-45</sup> peptide, using NMR chemical shift perturbations. As shown in the <sup>1</sup>H,<sup>15</sup>N HSQC spectra (Fig. 5, A and B), chemical shift variations upon lactose addition are significantly modified when λ5-UR<sup>22-45</sup> is present. This result indicates that lactose affinity for GAL1 is significantly reduced when GAL1 is bound to λ5-UR<sup>22-45</sup>. To illustrate these changes we focused on two GAL1 residues involved in key interactions with lactose, His-52, and Trp-68 (Fig. 5C). Both titration curves show lower chemical shift variations in the presence of λ5-UR<sup>22-45</sup>. K<sub>D</sub> evaluations in these experimental conditions led to a value of

FIGURE 1. NMR titration of GAL1/λ5-UR<sup>22-45</sup> interaction. A, <sup>1</sup>H, <sup>15</sup>N HSQC spectra of <sup>15</sup>N-labeled GAL1 (0.7 mM) in the absence (black) and in the presence of unlabeled λ5-UR<sup>22-45</sup> peptide at a concentration of 0.175 mM (red), 0.35 mM (green), 0.525 mM (blue), 0.7 mM (violet), 0.875 mM (magenta), and 1 mM (maroon). Spectra were recorded on a 600-MHz spectrometer at 303 K in 20 mM KPO<sub>4</sub> (pH 5.2), 100 mM NaCl, 1.4 mM lactose, 10% D<sub>2</sub>O, B, enlarged views of the <sup>1</sup>H, <sup>15</sup>N HSQC spectra for Arg-73, Glu-74, Asp-102, and Phe-106 peaks. C, plot of the chemical shift variations of Arg-73, Glu-74, Asp-102, and Phe-106 proton amide against λ5-UR<sup>22-45</sup> concentration. Titration curves were fitted with a sigmoidal function. The K<sub>D</sub> derived from the fit of the curves corresponds to 310 ± 65 µM. D, normalized chemical shift perturbations monitored in <sup>1</sup>H, <sup>15</sup>N HSQC spectra of <sup>15</sup>N-labeled GAL1 with a 2 molar excess of lactose following the addition of λ5-UR<sup>22-45</sup> (1 molar equivalent). The plot is color-coded according to the location of the residues (orange, side and back face; pink, CBS). The secondary structures of GAL1 are shown above the plot. E, GAL1 <sup>1</sup>H, <sup>15</sup>N HSQC chemical shift perturbations mapped onto the GAL1 dimer structure bound to lactose (PDB ID code 1GZW (15)) following the addition of the λ5-UR<sup>22-45</sup>. The residues exhibiting significant chemical shift variations in the presence of the λ5-UR<sup>22-45</sup> are labeled and colored according to the color code used in D. From left to right, back view, side view.

<figure><img src="image_11.png" /></figure>
Galectin-1/Pre-BCR Interaction

<figure><img src="image_12.png" /><figcaption>FIGURE 3. **Structure of λ5-UR<sup>22-45</sup> free and bound to GAL1.** A and B, ensemble of the backbone traces of the 20 superimposed lowest energy NMR structures of λ5-UR<sup>22-45</sup> (A) and of λ5-UR<sup>22-45</sup> bound to GAL1 (B). C, superimposition of regions extracted from two-dimensional ¹H NOESY spectra recorded on λ5-UR<sup>22-45</sup> (*red spectrum*) and two-dimensional ¹³C,¹⁵N-filtered NOESY recorded on ¹³C,¹⁵N-GAL1 complexed to λ5-UR<sup>22-45</sup> (*black spectrum*). Some correlations indicative of a α-helix conformation are labeled. Left panel zooms on an amide proton region; right panel presents some proton amide/α-proton correlations.</figcaption></figure>

0.907 ± 0.061 mM in the absence of the peptide and of 3.7 ± 0.3 mM in the presence of the peptide. Therefore, peptide binding to GAL1 induces a 4-fold decrease in the lactose binding activity of GAL1.

**Structural Analysis of the GAL1/λ5-UR<sup>22-45</sup> Complex Highlights Hydrophobic and Electrostatic Interactions**—As shown in Fig. 4, B and C, the entire length of the λ5-UR<sup>22-45</sup> α-helix (residues Arg-29 to Ser-39) is involved in the direct interaction with GAL1 in a completely buried surface area of 926.1 Å². Although the binding surface is mostly hydrophobic, it is surrounded by a number of charged residues. The GAL1-interacting surface consists primarily of residues from the β9 strand (Tyr-104, Glu-105, Phe-106, and Lys-107) with a number of residues from the β6 strand (Glu-74), loop β6–β7 (Val-76), loop β7–β8 (Asp-102), and loop β9–β10 (Asn-110). The Trp-30 side chain of the λ5-UR<sup>22-45</sup> peptide projects toward the center of the hydrophobic surface presented by GAL1 and is totally buried. The Trp-30 aromatic ring is surrounded by a triad of hydrophobic side chains from GAL1 (Val-76, Tyr-104,

and Phe-106) among which the Phe-106 aromatic ring is directly facing the Trp-30 ring of the λ5-UR<sup>22-45</sup>, thus contributing strongly to the hydrophobic interactions within the complex (Fig. 4C). Moreover, the additional aliphatic side chains, such as the Leu-26 of λ5-UR<sup>22-45</sup>, project along the peptide creating a hydrophobic surface on the helix, which complements the hydrophobic surface of GAL1 (Fig. 4C). Surprisingly, despite the high arginine content of the λ5-UR<sup>22-45</sup> peptide, only Arg-27 and Arg-29 in the N-terminal part of the λ5-UR<sup>22-45</sup> peptide are found engaged in salt bridges with the Asp-102 and Glu-74 acidic residues of GAL1, respectively (Fig. 4B). These acidic residues are located adjacent to, but on both sides of, the GAL1 hydrophobic surface (Fig. 4C). Therefore, these structural data reveal a dual mode for GAL1 binding to the λ5-UR<sup>22-45</sup> peptide, which involves both hydrophobic and electrostatic interactions.

**Hydrophobic Interactions Are Essential for Relocalization of the Pre-BCR at the Pre-B/Stromal Cell Synapse**—The clustering of pre-BCRs at the surface of the pre-BII cells is a prerequisite to
<figure><img src="image_13.png" /><figcaption>Galectin-1/Pre-BCR Interaction</figcaption></figure>

FIGURE 4. Structural model of the λ5-UR<sup>22–45</sup>/GAL1 complex. A, representation of the lowest energy structure of GAL1 bound to λ5-UR<sup>22–45</sup>. GAL1 is displayed as a semitransparent solvent-accessible surface with a ribbon model displayed below the surface. The GAL1 dimer is colored gray, and λ5-UR<sup>22–45</sup> is colored in red ribbon. B, ribbon view of the λ5-UR<sup>22–45</sup>/GAL1 complex with the side chains of the contacting residues shown and labeled. The GAL1 hydrophobic and acidic contacting residues are colored green and orange, respectively. The λ5-UR<sup>22–45</sup> residues are colored red, and the electrostatic interactions are represented as dashed lines. C, magnification of the GAL1 interaction surface (same orientation as in B) highlighting the hydrophobic (green) and electrostatic (orange) residues contacting the λ5-UR<sup>22–45</sup>. The mutated residues are labeled.

their activation, leading to the trans-phosphorylation of the
Igα/β molecules. Receptor clustering may occur in an autono-
mous manner (9) and/or may be induced by the bone marrow
microenvironment through the GAL1/pre-BCR interaction,
leading to pre-BCR relocalization (8). Based on the structural
model of the GAL1/λ5-UR<sup>22–45</sup> complex, we tested at a cellular
level the contribution of hydrophobic and electrostatic interac-
tions on the pre-BCR relocalization process using full-length
λ5-UR and two double λ5-UR mutants (λ5-UR-L26A/W30A
and λ5-UR-R27A/R29A). The pre-B cells were co-cultured
with stromal cells, and pre-BCR and GAL1 clustering was

<figure><img src="image_14.png" /></figure>

FIGURE 5. Lactose binding activity of GAL1 free and bound to λ5-UR<sup>22-45</sup>. A and B, <sup>1</sup>H, <sup>15</sup>N HSQC spectra of <sup>15</sup>N-labeled GAL1 (0.2 mM concentration) upon addition of increasing amount of lactose in the absence (A) and in the presence (B) of 3 molar equivalent of λ5-UR<sup>22-45</sup>. Spectra were recorded with 0 (black), 0.05 (skyblue), 0.1 (magenta), 0.15 (navy), 0.2 (red), 0.25 (lime), 0.3 (maroon), 0.4 (cyan), 0.8 (orange), 2 (dark gray), and 8 (pink) mM lactose. C, lactose titration curves in the absence (○) and in the presence (●) of λ5-UR<sup>22-45</sup> for His-52 and Trp-68 are shown. Chemical shift variations differences are plotted against lactose concentration.

observed at the pre-B/stromal cell contact site using confocal
microscopy (Fig. 6A). As reported previously, the addition of
the full-length λ5-UR into co-cultures inhibits the relocaliza-
tion process by 25% (7). In contrast, the addition of the λ5-UR-
L26A/W30A mutant has no effect on the pre-BCR relocaliza-
tion process compared with the native λ5-UR (Fig. 6B). This
result is significant (p value = 0.018), implying that hydropho-
Galectin-1/Pre-BCR Interaction

<figure><img src="image_15.png" /><figcaption>FIGURE 6. Analysis of pre-BCR relocalization. A, Nalm6 pre-B cells were co-cultured on OP9 stromal cells and were analyzed using confocal microscopy. The differential interferential contrast (DIC) image shows fixed pre-B/stromal cell conjugates. The staining was performed using goat anti-human IgM (red) and rabbit anti-GAL1 AS (green) antibodies. The red and the white stars indicate cells with a relocalized and a nonrelocalized pre-BCR, respectively. B, inhibition of pre-BCR relocalization in the presence of the λ5-UR and of the two λ5-UR mutants is shown. Three Independent experiments were performed, and the data are expressed as the percentage of inhibition of pre-BCR relocalization ± S.D. (error bars). Pre-BCR relocalization was inhibited 25% in the presence of recombinant λ5-UR. Similar inhibition levels have been reported (7) and were highly significant (p value = 0.008, data not shown). *, p = 0.018 between λ5-UR and λ5-UR-L26A/W30A and ns (not significant) between λ5-UR and λ5-UR-R27A/R29A (p = 0.07) and between λ5-UR-L26A/W30A and λ5-UR-R27A/R29A (p = 0.06).</figcaption></figure>

bic residues are crucial for the pre-BCR relocalization. Surprisingly, pre-BCR relocalization in the presence of the λ5-UR-R27A/R29A mutant is not significantly diminished (Fig. 6B). These results suggest that the charged and the hydrophobic residues tested have not the same contribution on the relocalization process.

To evaluate at a molecular level the importance of the hydrophobic and electrostatic interactions, the effect of replacing the interacting residues on the formation of the complex was tested using NMR (supplemental Figs. S5 and S6). To target the hydrophobic core of the complex, we used two complementary mutants, GAL1-Y104A/F106A and λ5-UR²²⁻⁴⁵-L26A/W30A. The effect of the hydrophobic substitutions was significant, resulting in a strong impairment of the complex formation (supplemental Fig. S6, B and C). We also targeted the two electrostatic contacts participating in the complex formation using the GAL1-E74A/D102A and the λ5-UR²²⁻⁴⁵-R27A/R29A mutants. These mutations had a lesser effect on the complex formation (supplemental Fig. S6, D and E), as suggested by the results from the pre-BCR relocalization assays (Fig. 6B). Therefore, the pre-BCR relocalization and the NMR experiments show that the GAL1/pre-BCR complex formation and the GAL1-induced pre-BCR relocalization are hydrophobically driven with a minor contribution from electrostatic contacts.

DISCUSSION

We have identified a minimal GAL1-interacting region within the 45 residues of the full-length λ5-UR. One important finding of our study is that the central region (residues 29–39) adopts a stable helical conformation, which preexists in solution prior binding to GAL1 (Fig. 3). It is so far the first detailed structural information about the λ5-UR. Structural data of the human GAL1 in complex with λ5-UR²²⁻⁴⁵ (Fig. 4) revealed that the central feature of the binding is the involvement of the λ5-UR²²⁻⁴⁵ α-helix over its entire length in the interaction with the GAL1 binding surface. Complex formation is mainly mediated by hydrophobic residues present on both partners and, at a lower extent, by electrostatic contacts involving two arginines on λ5-UR²²⁻⁴⁵ and two acidic residues on GAL1. Moreover, we found that the hydrophobic contribution to GAL1/λ5 complex formation is critical for pre-BCR relocalization when pre-B cells are co-cultured with GAL1⁺ stromal cells (Fig. 6), validating our structural data in a biological context. These results represent the first atomic view of a pre-BCR/ligand interaction.

Besides its carbohydrate binding activity, GAL1 has also been described as being engaged in protein interactions in a carbohydrate-independent manner (13). These interactions occur mainly in the intracellular compartments whereas its carbohydrate binding activity is mostly extracellular. So far, the λ5 protein from the pre-BCR is a unique example of a nonglycosylated protein partner of GAL1 described in the extracellular matrix. Among the intracellular nonglycosylated partners of GAL1, actin (25, 26), Gemin4 (27, 28), and Ras (29, 30) involved in a variety of intracellular functions have been identified. These proteins do not share consensus amino acid sequence or structural motifs, and neither the GAL1 sites nor the structural determinants that are involved in these interactions have been established yet. In the case of H-Ras/GAL1 interactions, Rotbalt *et al.* identified a hydrophobic surface on GAL1 possessing isoprenoid-binding residues essential for Ras-GTP stabilization and its association with the membrane (30). This surface, which has been proposed to interact with the farnesyl group of Ras, lies within the GAL1 β-sheet and includes residues Leu-9, Leu-11, Leu-17, Phe-30, Phe-32 and Ile-128. Thus, λ5 interacting surface on GAL1 is different from this proposed Ras farnesyl binding site.

The other feature revealed by our studies is the close proximity of λ5 interacting surface with the CBS on GAL1 (Fig. 4). During synapse formation, GAL1 has a central role by interacting with the pre-BCR through a protein/protein interaction, but also with glycosylated counterreceptors through protein-carbohydrate recognition (8). We have observed that the absence of lactose has no significant effect on λ5-UR²²⁻⁴⁵ binding to GAL1 (Fig. 2A). This is in agreement with our previous observations that there is no direct competition of SLC and carbohydrates for GAL1 binding (8) and that GAL1 is able to interact at the same time with λ5 (protein/protein interaction) and with integrins (protein/carbohydrate interaction) during synapse formation (11). On the other hand, we have shown that λ5-UR²²⁻⁴⁵ binding to GAL1 induces a 4-fold decrease in the lactose binding activity of GAL1 (Fig. 5). Moreover, some residues belonging to GAL1 CBS undergo chemical shift variations
Galectin-1/Pre-BCR Interaction

<figure><img src="image_16.png" /></figure>

FIGURE 7. Sequence and structural analysis of λ5-UR. A, conserved residues of the λ5-UR sequence in various species are shown in the red boxes. Above the sequences, the residues involved in the GAL1 interaction are labeled with red dots; below the sequences, the λ5-UR arginines involved in autonomous pre-BCR oligomerization are labeled with blue dots (9). B, bound λ5-UR<sup>22-45</sup> structure shows λ5-UR<sup>22-45</sup> residues interacting with GAL1 colored red, the conserved arginines involved in autonomous pre-BCR oligomerization are colored blue, GAL1 hydrophobic residues are labeled in green, and electrostatic residues are in orange.

upon λ5-UR<sup>22-45</sup> binding to GAL1 (Figs. 1D and 2A), suggesting modifications of the CBS upon λ5 binding.

Another known example of a GAL1/peptide interaction is the binding of the nonnatural peptide Anginex (31). This peptide is a powerful antiangiogenic molecule with antitumor activity because it blocks adhesion and migration of activated endothelial cells thus preventing angiogenesis *in vitro* and *in vivo* (31–33). Recent studies (34) have shown that binding of Anginex to GAL1 greatly enhances the affinity of GAL1 for certain ligands, including biologically relevant glycoproteins. Moreover, a topomimetic of Anginex has been shown to be an allosteric inhibitor of GAL1 lactose binding while interacting with a surface of GAL1 located on the back face of the protein (35). This implies that Anginex could have opposite effect on GAL1 depending on the carbohydrate substrate tested. In our system, the exact oligosaccharide ligands of GAL1 at the cell surface of stromal and pre-BII cells have not been identified. Thus, it is not excluded that λ5-UR could have an effect similar to that of Anginex on GAL1 at the synapse. Indeed, modulation of GAL1 affinity for specific glycoproteins, either enhanced or reduced, could be a strategic step at the synapse level to modify the cross-linked lattices. These modifications of the interacting network could be crucial during pre-BCR oligomerization and subsequent receptor internalization.

The sequence analysis emphasizes that the GAL1 hydropho-
bic and charged amino acids involved in the λ5 interacting site
are highly conserved among species (supplemental Fig. S7).
Similarly, the human and primate λ5 sequences are well con-
served, although differences in the rat and mouse sequences
exist (Fig. 7A). The main difference observed is the replacement

in rat and mouse of the human Arg-27 and Arg-29 by Trp and
Leu hydrophobic residues, respectively, thus enhancing the
hydrophobic interacting potential of this region. Moreover, we
have shown that these two arginines are not critical for pre-
BCR relocalization (Fig. 6).

Previous studies in mice revealed a crucial role for conserved λ5-UR arginines (Arg-9, Arg-11, Arg-23, Arg-32, Arg-39, Arg-44, and Arg-49) in the autonomous pre-BCR oligomerization (9). Indeed, deletion of full-length λ5-UR or mutations of these conserved arginines led to an increase in pre-BCR cell surface expression and to a decrease in pre-BCR clustering and internalization. Here, the GAL1/λ5-UR<sup>22-45</sup> complex clearly shows that Arg-23, Arg-32, and Arg-44 are not facing the GAL1 interacting surface, but in contrast to the λ5-UR<sup>22-45</sup> residues delin-eating the hydrophobic interacting surface, they are solvent-exposed (Fig. 7B). Our data indicate that from a structural point of view, autonomous and GAL1-induced pre-BCR oligomeriza-tions do not involve the same set of residues; these processes are therefore not in competition and could instead be com-plementary. However, it remains unclear under which con-ditions during pre-BII cell differentiation and/or selection of the V<sub>H</sub> repertoire a unique activation mode or a combination of both modes is required. A hypothesis could be that con-stitutive pre-BCR activation which has been recently shown to be very low (36) is helped by the stromal-derived GAL1, hence strengthening the pre-BCR oligomerization leading to synapse formation and optimal pre-BCR activation. The requirement of GAL1 could be linked to the level of pre-BCR surface expression. In the case of a low pre-BCR surface expression, as is mainly the case for normal pre-BII cells *in*
Galectin-1/Pre-BCR Interaction

vivo, GAL1-induced signaling would be required to amplify pre-BCR functions, whereas in the case of a high receptor expression, GAL1 could not be necessary for pre-BCR oligomerization. These studies provide the first molecular snapshot of a pre-BCR/ligand interaction that promotes pre-BCR clustering leading to pre-BCR activation.

Acknowledgments—We thank Caroline Breton for help with the pre-BCR relocalization experiments, Stéphane Mancini for contributions to the statistical analysis, and Corinne Sebban-Kreuzer for help with the K<sub>D</sub> calculation. We thank the TGE RMN THC (Trés Grands Equipements de Résonance Magnétique Nucléaire à Trés Haut Champs) FR3050 for performing the research.

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<figure><img src="image_17.png" /></figure>
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<figure><img src="image_18.png" /></figure>