# Full-Length Genome Sequence of a Sindbis Virus Strain Isolated from Culex cinereus in 1977 in Bozo, Central African Republic

Rita Sem Ouilibona, Huguette Tchetgna Simo, Ulrich Vickos, Nicolas Berthet, Emmanuel Nakouné

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Rita Sem Ouilibona, Huguette Tchetgna Simo, Ulrich Vickos, Nicolas Berthet, Emmanuel Nakouné. Full-Length Genome Sequence of a Sindbis Virus Strain Isolated from Culex cinereus in 1977 in Bozo, Central African Republic. Genome Announcements, 2018, 6 (26), 10.1128/genomeA.00455-18. pasteur-03516012

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# Full-Length Genome Sequence of a Sindbis Virus Strain Isolated from *Culex cinereus* in 1977 in Bozo, Central African Republic

Rita C. Sem Ouilibona,ª Huguette D. Tchetgna Simo,ᵇ Ulrich Vickos,ª Nicolas Berthet,ᵇ,c,d Emmanuel Nakounéª

**ABSTRACT** We report here the complete genome sequence of a Sindbis virus (SINV) strain, ArB7761, isolated in 1977 in the Central African Republic. This strain, closely related to the Babanki virus, belongs to the SINV genotype I clade. However, it differs from the Egyptian prototype strain AR339 by several indels in the nsP3 gene.

The Sindbis virus (SINV) is the prototype of the arthropod-borne virus (arbovirus) genus *Alphavirus*, a member of the *Togaviridae* family. In humans, SINV infections are mainly benign, consisting of fever, rash, arthralgia, and myalgia (1), but no virus-specific vaccines or therapies for the treatment of this disease are currently available (2). Children are often infected but develop only subclinical disease, while clinical symptoms are more prevalent in adults. Morbidity is highest in women between 45 and 65 years of age (3). This zoonotic virus is maintained in nature through a transmission cycle involving birds and *Culex* mosquitos. Since its initial description in Egypt in 1952, SINV has been found in Africa, Asia, Australia, and Europe (Scandinavia and Russia) (4), although clinical infections and outbreaks have been described in South Africa and northern Europe only. Currently, six geographically distinct genotypes of SINV are recognized, but the virus is sometimes referred to as Babanki, Ockelbo, Karelian fever, Kyzylagach, or Whataroa, depending on location.

SINV strain ArB7761 was isolated on suckling mice from a pool of *Culex cinereus* mosquitoes collected in a forested area in 1977 at Bozo (05°18′N, 18°28′E), Central African Republic (CAR). The lyophilized virus was resuspended in phosphate-buffered saline and amplified on Vero E6 cell lines. Cytopathic effects were observed 2 to 3 days postinfection, and then the harvested supernatant was used for RNA extraction using the QIAamp viral RNA minikit (Qiagen). Double-stranded cDNA was synthetized and used for paired-end library construction with the NEBNext Ultra RNA library prep kit for Illumina (New England BioLabs, UK). High-throughput sequencing was performed on a MiSeq instrument (Illumina). A total of 4,685,922 paired-end reads were obtained, and 195,973 reads corresponded to SINV. Analysis revealed a sequence of 11,709 bp in length. Our sequence shows 99% similarity with the Babanki virus (GenBank accession number HM147984) from Cameroon and 94% similarity with the AR339 prototype SINV strain (J02363). At the amino acid level, similar to most alphaviruses, there is an additional stop codon between genes for nonstructural proteins nsP3 and nsP4. Compared with AR339, five amino acids (codons) (M, Q, E, A, and D) are inserted at positions 1786, 1787, 1788, 1799, and 1800 of the nonstructural polyprotein nsP123, respectively, whereas three amino acids (codons) (A, V, and Q) are deleted at positions 1827, 1828, and 1829 at the end of nsP3, respectively. These mutations been already

Received 16 May 2018 **Accepted** 21 May 2018 **Published** 28 June 2018

**Citation** Sem Ouilibona RC, Tchetgna Simo HD, Vickos U, Berthet N, Nakouné E. 2018. Full-length genome sequence of a Sindbis virus strain isolated from *Culex cinereus* in 1977 in Bozo, Central African Republic. Genome Announc 6:e00455-18. https://doi.org/10.1128/ genomeA.00455-18.

**Copyright** © 2018 Sem Ouilibona et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

Address correspondence to Emmanuel Nakouné, enakouney@gmail.com. N.B. and E.N. co-led this study.

<footnote>ªInstitut Pasteur de Bangui, Bangui, Central African Republic</footnote>

<footnote>ᵇCentre Interdisciplinaire de Recherches Médicales de Franceville (CIRMF), Franceville, Gabon</footnote>

<footnote>ᶜInstitut Pasteur, Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence, Paris, France</footnote>

<footnote>ᵈCentre National de Recherche Scientifique (CNRS) UMR3569, Paris, France</footnote>
demonstrated in other SINV strains (5). Furthermore, 22 and 57 amino acid changes were observed in the structural and nonstructural polyproteins, respectively. A maximum likelihood phylogenetic tree shows that SINV_CAR_1976 belongs to genotype I of SINV, along with the other African strains (6).

In conclusion, these molecular data on our new SINV isolate support the wide distribution of this virus in Africa. However, given our limited knowledge of this virus and the reasons why cases remain limited to a few regions, further studies are required to better understand its transmission cycles.

Accession number(s). The complete genome sequence of SINV strain ArB7761 was deposited in GenBank under the accession number MH212167.

## ACKNOWLEDGMENTS

We thank the members of the Arbovirus and Hemorrhagic Fever Virus laboratories at the Institut Pasteur of Bangui in the Central African Republic for their useful comments and suggestions. We are also grateful to the Centre Interdisciplinaire de Recherches Médicales de Franceville, in Gabon, for its high-throughput sequencing service.

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