# Corrigendum: Genetic variation in host-specific
competitiveness of the symbiont Rhizobium
leguminosarum Symbiovar viciae

Stéphane Boivin, Frederic Mahé, Frédéric Debellé, Marjorie Pervent, Mathilde
Tancelin, Marc Tauzin, Jerzy Wielbo, Sylvie Mazurier, Peter Young, Marc
Lepetit

► To cite this version:

Stéphane Boivin, Frederic Mahé, Frédéric Debellé, Marjorie Pervent, Mathilde Tancelin, et al. Corrigendum: Genetic variation in host-specific competitiveness of the symbiont Rhizobium leguminosarum Symbiovar viciae. Frontiers in Plant Science, 2025, 16, pp.1542763. 10.3389/fpls.2025.1542763. hal-04999872

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EDITED AND REVIEWED BY
Andrea Genre,
University of Turin, Italy

*CORRESPONDENCE
Marc Lepetit

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marc.lepetit@inrae.fr

RECEIVED 10 December 2024

ACCEPTED 05 February 2025

PUBLISHED 24 February 2025

CITATION

Boivin S, Mahé F, Debellé F, Pervent M, Tancelin M, Tauzin M, Wielbo J, Mazurier S, Young P and Lepetit M (2025) Corrigendum: Genetic variation in host-specific competitiveness of the symbiont *Rhizobium leguminosarum* Symbiovar *viciae*. *Front. Plant Sci.* **16**:1542763.
doi: 10.3389/fpls.2025.1542763

COPYRIGHT

© 2025 Boivin, Mahé, Debellé, Pervent, Tancelin, Tauzin, Wielbo, Mazurier, Young and Lepetit. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

# Corrigendum: Genetic variation in host-specific competitiveness of the symbiont *Rhizobium leguminosarum* Symbiovar *viciae*

Stéphane Boivin<fnref n="1" />, Frederic Mahé², Frédéric Debellé³, Marjorie Pervent<fnref n="1" />, Mathilde Tancelin<fnref n="1" />, Marc Tauzin<fnref n="1" />, Jerzy Wielbo⁴, Sylvie Mazurier⁵, Peter Young⁶ and Marc Lepetit<fnref n="1" />,⁷*

KEYWORDS

*Rhizobium leguminosarum* symbiovar *viciae*, competitiveness, pea, fababean, lentil, Fabeae, DNA metabarcoding, symbiosis

## A Corrigendum on

### Genetic variation in host-specific competitiveness of the symbiont *Rhizobium leguminosarum* Symbiovar *viciae*

By Boivin S, Mahé F, Debellé F, Pervent M, Tancelin M, Tauzin M, Wielbo J, Mazurier S, Young P and Lepetit M (2021) *Front. Plant Sci.* **12**:719987. doi: 10.3389/fpls.2021.719987

In the published article Mutch et al., 2004, Young et al., 2006, Laguerre et al., 2007; Riah et al., 2014; Reeve et al., 2015, Seshadri et al., 2015, Sánchez-Cañizares et al., 2018; Jorrin et al., 2020 were not cited in the article. The citation has now been inserted in the **Result** section, first paragraph and should read:

> "At the beginning of this study, we collected 73 genome sequences of Fabeae symbionts available in GenBank (Supplementary Table 1). To increase the diversity, the genomes of 48 additional rhizobia from diverse geographical origins, and/or carrying diverse sequences of the symbiotic marker nodD belonging to the symbiovar (sv) viciae, were also sequenced (Supplementary Table 1). All rhizobia have been isolated in previous studies from *Pisum sativum*, *Vicia faba*, *Lens culinaris*, and *Lathyrus pratensis* root nodules (Supplementary Table 1; Mutch et al., 2004, Young et al., 2006, Laguerre et al., 2007; Riah et al., 2014; Reeve et al., 2015, Seshadri et al., 2015, Sánchez-Cañizares et al., 2018; Jorrin et al., 2020). Most of the bacteria (117/121) shared an Average Nucleotide Identity (ANI) >92% (Figure 1, Supplementary Table 8). They belonged to nine of the *R. leguminosarum* complex (*Rlc*) genospecies previously described: gsB, gsC, gsD, gsE, gsG, gsN, gSO, gsQ, and gsR (Young

<footnote n="1">¹Laboratoire des Symbioses Tropicales et Méditerranéennes, INRAE, IRD, CIRAD, Montpellier SupAgro, University of Montpellier, Montpellier, France, ²Biologie et Génétique des Interactions Plante-Parasite, CIRAD, INRAE, Montpellier SupAgro, University of Montpellier, Montpellier, France, ³Laboratoire des Interactions Plantes-Microorganismes, INRAE, CNRS, University of Toulouse, Castanet-Tolosan, France, ⁴Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland, ⁵Agroecology, AgroSup Dijon, INRAE, University Burgundy Franche-Comté, Dijon, France, ⁶Department of Biology, University of York, York, United Kingdom, ⁷Institut Sophia Agrobiotech, INRAE, CNRS, Côte d'Azur University, Sophia-Antipolis, France</footnote>
et al., 2021). Four other strains were phylogenetically distant from the others (88% < ANI < 90%; Supplementary Table 8) and hence outside the *Rlc*, but included inside the *R. leguminosarum-etli* clade according the recent study of Young et al. (2021).”.

In the published article, the respective original sources of the newly 48 sequenced Rlv strains were ambiguous.

A correction has been made to **Material and method** section, Bacterial Collection, Inoculation and Plant Growth Conditions subsection, first paragraph, first sentence. This sentence previously stated:

"Rhizobia isolated from Pisum sativum, Vicia faba, Lens culinaris, or Lathyrus pratensis root nodules, and from various geographical origins, were collected (Supplementary Table 1)"

The corrected sentence appears below:

"Rhizobia isolated in previous studies by several laboratories from Pisum sativum, Vicia faba, Lens culinaris, or Lathyrus pratensis root nodules, and from various geographical origins, were used in this study (Supplementary Table 1)."

A correction has been made to **Acknowledgment** section, Bacterial Collection, Inoculation and Plant Growth Conditions subsection, first paragraph, first sentence. This sentence previously stated:

"We acknowledge Markus Braun (Heidelberg University), Xinhua Sui and Changfu Tian (China Agricultural University, Beijing), Anna Skorupska and Andrzej Mazur (Medical University of Lublin), and Juan Imperial (Instituto de Ciencias Agrarias, Madrid) for providing some of the rhizobial strains

included in this study, as well as the Genotoul GeT-PlaGe platform of Toulouse for the Illumina MiSeq sequencing.”

The corrected sentence appears below:

"We acknowledge Markus Braun (Heidelberg University), Xinhua Sui and Changfu Tian (China Agricultural University, Beijing), Anna Skorupska and Andrzej Mazur (Medical University of Lublin), Juan Imperial (Instituto de Ciencias Agrarias, Madrid), Nassira Riah (University of Constantine) for providing some of the rhizobial strains included in this study, as well as the Genotoul GeT-PlaGe platform of Toulouse for the Illumina MiSeq sequencing.”

In the published article, there was an error in Supplementary Table 1, the sources of the newly 48 sequenced Rlv strains were not properly detailed.

The authors apologize for these errors and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

## Publisher's note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

## References

Jorrin, B., Palacios, J. M., Peix, Á., and Imperial, J. (2020). Rhizobium ruizarguesonis sp. nov., isolated from nodules of Pisum sativum L. *System. Appl. Microbiol.* 43, 126090. doi: 10.1016/j.syapm.2020.126090

Laguerre, G., Depret, G., Bourion, V., and Duc, G. (2007). Rhizobium leguminosarum bv. viciae genotypes interact with pea plants in developmental responses of nodules, roots and shoots. *New Phytol.* 176, 680–690. doi: 10.1111/j.1469-8137.2007.02212.x

Mutch, L. A., and Young, J. P. W. (2004). Diversity and specificity of Rhizobium leguminosarum biovar viciae on wild and cultivated legumes. *Mol. Ecol.* 13, 2435–2444. doi: 10.1111/j.1365-294X.2004.02259.x

Reeve, W., Ardley, J., Tian, R., Eshragi, L., Yoon, J. W., Ngamwisetkun, P., et al. (2015). A Genomic Encyclopedia of the Root Nodule Bacteria: assessing genetic diversity through a systematic biogeographic survey. *Standards Genom. Sci.* 10, 14. doi: 10.1186/1944-3277-10-14

Riah, N., Béna, G., Djekoun, A., Heulin, K., de Lajudie, P., and Laguerre, G. (2014). Genotypic and symbiotic diversity of Rhizobium populations associated with cultivated lentil and pea in sub-humid and semi-arid regions of Eastern Algeria. *Syst. Appl. Microbiol.* 37, 368–375. doi: 10.1016/j.syapm.2013.12.008

Sánchez-Cañizares, C., Jorrín, B., Durán, D., Nadendla, S., Albareda, M., Rubio-Sanz, L., et al. (2018). Genomic diversity in the endosymbiotic bacterium rhizobium leguminosarum. *Genes* 9, 60. doi: 10.3390/genes9020060

Seshadri, R., Reeve, W. G., Ardley, J. K., Tennessen, K., Woyke, T., Kyrpides, N. C., et al. (2015). Discovery of novel plant interaction determinants from the genomes of 163 root nodule bacteria. *Sci. Rep.* 5, 16825. doi: 10.1038/srep16825

Young, J. P., Crossman, L. C., Johnston, A. W., Thomson, N. R., Ghazoui, Z. F., Hull, K. H., et al. (2006). The genome of Rhizobium leguminosarum has recognizable core and accessory components. *Genome Biol.* 7 (4). doi: 10.1186/gb-2006-7-4-r34