# Diet-induced hyperglycaemia alters the hepatic microRNA profile in rainbow trout, O. mykiss

Jan A. Mennigen, Stéphane Panserat, Lucie Marandel

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Jan A. Mennigen, Stéphane Panserat, Lucie Marandel. Diet-induced hyperglycaemia alters the hepatic microRNA profile in rainbow trout, O. mykiss. PWH Symposium (satellite symposium preceding the 56th annual meeting of the Canadian Society of Zoologists), May 2017, Winnipeg, Canada., pp.27, 2017, PWH Symposium schedule and abstracts. hal-01602644

HAL Id: hal-01602644

https://hal.science/hal-01602644

Submitted on 2 Jun 2020

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<figure><img src="image_1.png" /></figure>
**3.3 Diet-induced hyperglycemia alters the hepatic microRNA profile in rainbow trout, O. mykiss.**

Jan A. Mennigen<fnref n="1" />, Stephane Panserat<fnref n="2" /> and Lucie Marandel<fnref n="2" />

<footnote n="1">¹Department of Biology, University of Ottawa, K1N6A5, Ottawa, Ontario, Canada.</footnote>

<footnote n="2">²Institut National de la Recherche Agronomique, Aquapôle, 64310 Saint-Pée-sur-Nivelle, Aquitaine, France
MicroRNAs are small, non-protein coding RNAs, which have emerged as potent regulators of glucose
metabolism in mammalian model species through destabilization of specific mRNAs and inhibition of
protein translation. While many miRNAs are highly conserved in evolution, teleost fish possess additional
lineage- and species-specific miRNAs, targeting a potentially vastly different set of mRNA targets. To gain
insight into the role of miRNA networks in the 'glucose-intolerant' phenotype in rainbow trout, we profiled
hepatic miRNA abundance in the liver of rainbow trout exhibiting diet-induced normo-and hyperglycemia.
Our study identified a total of 26 differentially regulated miRNAs, among them several previously identified
as being regulated in a hyperglycemic environment in mammalian models. While this suggests at least some
regulatory similarities between miRNA regulation in hyperglycemic rainbow trout and mammalian models,
predicted mRNA targets suggest a large degree of divergence. Using our identified miRNA targets, future
studies will focus on possible causative roles for select hepatic miRNAs towards the glucose-intolerant
phenotype in rainbow trout.</footnote>