DESIGNING AND BUILDING YEAST FOR THE FUTURE

Isak S. Pretorius

Chancellery, Macquarie University, Balaclava Road, Sydney, NSW 2109, Australia

Contact email: Sakkie.Pretorius@mq.edu.au

Synthetic Biology is poised to shift yeast genetics and strain development from a ‘reading DNA’ to a ‘writing DNA’ paradigm. Some researchers are already going beyond sequencing and modifying genes; they are designing and building entire genomes from scratch. They are taking genetic and metabolic engineering to the next level – genome engineering. Following the development of the first ‘synthetic’ virus (poliovirus in 2002) and bacterium (Mycoplasma mycoides in 2010), yeast has just become the latest organism to be equipped with a fully synthetic chromosome. This breakthrough marks the first fully functional designer eukaryotic chromosome. The global *Yeast 2.0* project aims to build the ultimate yeast genome by 2017. With a genome size of ~12 Mb – distributed along 16 chromosomes that encompass ~6000 of which ~5000 are individually nonessential – *Saccharomyces cerevisiae* is poised to become the world’s first eukaryote with a chemically-synthesised genome. In designing the *Sc2.0* strain, the natural yeast genome will be streamlined by building in sites that will make it possible to reshuffle the genome at will, potentially yielding more desirable properties. This will pave the way for the design of ‘neo-chromosomes’ – purpose-built for specific applications. But for now, advances with the *Sc2.0* project (and with constructive input from a wide range of other disciplines, including Informatics, Engineering and Bio-ethics) will be of interest to the entire emerging field of Synthetic Biology. Each innovative contribution will form part of the thousands of tiny waves that drive the tides of progress towards the goal of understanding complex lifeforms and systems that will ultimately have a positive impact on the environment, economy and all of society.
POPULATION GENOMICS OF WILD YEAST AND THE ORIGIN OF WINE STRAINS

Ping Liu<fnref n="1" />, Sofia Dashko<fnref n="2" />, Helena Volk<fnref n="2" />, Lorena Butinar<fnref n="2" />, Jure Piškur<fnref n="2" />,<fnref n="3" />, **Justin Fay**<fnref n="1" />

Contact email: jfay@wustl.edu

*Saccharomyces* are frequently found in association with trees, particularly *Quercus* species. In addition to trees, *Saccharomyces cerevisiae* is also found in vineyard soil, grape and wine must. While most wine strains of *S. cerevisiae* have a European origin, *S. cerevisiae* is not often found on trees in Europe, Portugal and Spain being exceptions. As such, the origin of wine yeast is not known. In this study, we surveyed vineyards and forests in Slovenia for *Saccharomyces* species. We found both *S. cerevisiae* and *S. paradoxus* within and outside of vineyards. However, *S. cerevisiae* was more rare in forest samples. Population genomic analysis of these yeast strains will be used to test whether vineyard and forest populations are genetically differentiated from one another.

<footnote n="1">¹Department of Genetics, Washington University, St. Louis, MO, USA</footnote>

<footnote n="2">²Wine Research Center, University of Nova Gorica, Vipava, Slovenia</footnote>

<footnote n="3">³Department of Biology, Lund University, Lund, Sweden</footnote>
ACQUIRING NOVEL PHENOTYPES OF YEAST THROUGH ADAPTIVE
LABORATORY EVOLUTION

Jens Nielsen¹,²

¹ Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden

² Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK-2800 Lyngby, Denmark

The yeast *Saccharomyces cerevisiae* is widely used for production of fuels, chemicals, pharmaceuticals and materials. Through metabolic engineering of this yeast a number of novel new industrial processes have been developed over the last 10 years. Besides its wide industrial use, *S. cerevisiae* serves as an eukaryal model organism, and many systems biology tools have therefore been developed for this organism.

Despite our extensive knowledge of yeast metabolism and its regulation we are still facing challenges when we want to engineer complex traits, such as improved tolerance to toxic metabolites like butanol and elevated temperatures or when we want to engineer the highly complex protein secretory pathway. In this presentation it will be demonstrated how we can combine directed evolution with systems biology analysis to identify novel targets for rational design-build-test of yeast strains that have improved phenotypic properties. Examples will be on identifying targets for improving tolerance towards butanol and increased temperature and for improving secretion of heterologous proteins.
<figure><img src="image_15.png" /></figure>
# Authors index

## A

Abashidze, Ekaterine ... 175, 180

Abbas, Charles A. ... 28, 52

Abbondio, Marcello ... 134

Addis, Maria Filippa ... 134

Agaphonov, Michael O. ... 48

Ağırman, Bilal ... 109

Aguera, Evelyne ... 87

Aguilar, Pablo S. ... 39

Albers, Eva ... 41

Albertyn, Jacobus ... 164

Alcazar, Montserrat ... 111

Alkema, Wynand ... 155

Almutawa, Qamar ... 141

Alonzo, Amairani ... 111

Ambrožič, Tadeja ... 163

Amillastre, Emilie ... 173

Andersen, Mikael Rørdam ... 185

Ando, Masayasu ... 171

Andrews, Brenda J ... 67, 68, 199

Arana Sánchez, Alejandro ... 173

Aranda, Agustín ... 43, 161

Arellano, Melchor ... 110

Arneborg, Nils ... 144, 153

Arrizon, Javier ... 111, 173

Ask, Magnus ... 42

## B

Baerends, Richard J. S. ... 77

Bai, Feng-Yan ... 23

Bakeeva, Albina ... 53, 57

Bakek, Adam ... 153

Banilas, Georgios ... 35

Baronian, Keith ... 129

Barré, Benjamin ... 181

Barrio, Eladio ... 13

Barry, Kerrie ... 117

Barton, Dave ... 182

Bauer, Florian F. ... 101, 175, 176

Belák, Ágnes ... 132

Belo, Edgard ... 12

Beltran, Gemma ... 36, 188

Berezka, Krzysztof ... 60

Bergström, Anders ... 181

Berna, Luisa ... 39, 136

Berovič, Marin ... 112

Bettiga, Maurizio ... 42, 120, 121, 127

Bhutada, Govindprasad ... 125, 126

Bigey, Frédéric ... 37, 107, 108

Binti Mat Nanyan, Noreen Suliani ... 46

Biron, C-H. ... 131

Bischoff, Felix ... 129

Blomqvist, Johanna ... 57, 58, 60

Blondin, Bruno ... 157

Bódi, Zoltán ... 69

Boekhout, Teun ... 23, 148

Böer, Eric ... 83

Boido, Eduardo ... 39
Boles, Eckhard ... 80
Bols, Cristian - Marie ... 131
Bond, Ursula ... 138
Bonomi, Francesco ... 166
Boone, Charles ... 67, 68, 200
Booyse, Mardé ... 103
Boross, Gábor ... 69, 195
Boyacı Gündüz, Cennet Pelin ... 109
Brandenburg, Jule ... 58
Branduardi, Paola ... 81, 75
Brickwedde, Anja ... 76
Brloznik, Mojca ... 128, 175, 179
Brueckner, Christine ... 80
Bruschi, Carlo V. ... 186
Budroni, Marilena ... 108, 133, 134
Bun-ya, Masanori ... 122
Buser, Claudia C. ... 198
Butinar, Lorena ... 106, 202

C, Č

Cabaroğlu, Turgut ... 109
Cabral, Sara ... 165
Čadež, Neža ... 105
Cadiere, Axelle ... 107
Camarasa, Carole ... 78, 87, 107
Cambon, Brigitte ... 107
Camesasca, Laura ... 39
Campanaro, Stefano ... 44
Cano, María ... 43
Canonico, Laura ... 104
Čaplová, Júlia ... 135
Capusoni, Claudia ... 146
Cardinali, Gianluigi ... 32, 34, 116, 146, 175, 178

Carlin, Silvia ... 150
Carly, Frederic ... 169
Carmona, Lourdes ... 33
Carrau, Francisco ... 39
Carter, Zorana ... 140
Carvalho, Ângela ... 77
Caudy, Amy A. ... 175
Cavalieri, Duccio ... 136
Čertík, Milan ... 124, 135
Chamas, Alexandre ... 129
Chen, Yiqun ... 68
Chibana, Hiroji ... 122
Chiniquy, Jennifer ... 117
Chipashvili, Ramash ... 175, 180
Chorianopoulos, Nikolaos ... 35
Ciani, Maurizio ... 104
Claudia Colabella ... 116
Coi, Anna Lisa ... 108, 133
Colabella, Claudia ... 32, 178
Colon, Maritrini ... 197
Comi, Giuseppe ... 38
Comitini, Francesca ... 104
Compagno, Concetta ... 146, 149, 166, 175, 177, 193
Corich, Viviana ... 44
Corte, Laura ... 32, 34, 116, 146, 175, 178
Costanzo, Michael ... 68
Cote, Atina ... 34
Coupland, Paul ... 181
Cubillos, Francisco ... 33
Cutzu, Raffaela ... 134
Cuvelier, Roland ... 131
D

D'Avino, Luigi ... 174
Dapporto, Leonardo ... 136
Daran-Lapujade, Pascale ... 76
Daran, Jean-Marc ... 76
Dashko, Sofia ... 106, 179, 202
De Baros Pita, Will ... 54
De Carvalho, Bruna Trindade ... 12
De Filippo, Carlotta ... 136
De Garcia, Virginia ... 25
De Hulster, Erik A.F. ... 76
De Lorenzis, Gabriella ... 34
De Morais, Marcos Antonio ... 54
De Smidt, Olga ... 164
Delage, Laura ... 110
Dellacassa, Eduardo ... 39
Delneri, Daniela ... 71, 140, 141, 142, 194
Demeke, Mekonnen ... 12
Den Abt, Tom ... 12
Denega, Iryna O. ... 50
Dequin, Sylvie ... 37, 78, 79, 107, 108
Deshpande, Raamesh ... 68
Di Cagno, Raffaella ... 116
Di Paola, Monica ... 136
Dicks, Jo ... 113
Divol, Benoit ... 175, 176
Dmytruk, Kostyantyn ... 52, 55, 56, 59, 60, 61, 62, 63, 64, 65, 66
Donaldson, Ian ... 140
Driessen, Maureen R.M. ... 76
Du Preez, James C. ... 164
Du Toit, Maret ... 175, 176
Dumortier, Françoise ... 12
Dungrattanalert, Kobchai ... 142
Duraiswamy, Varuni ... 42

Durbin, Richard ... 181
Durieux, Alain ... 131
Dutoit, Raphaël ... 131

E

Eder, Matthias ... 78
Ekberg, Jukka ... 82
Elliston, Adam ... 113
Eriksson, Leif A ... 127
Erten, Hüseyin ... 109
Esteve-Zarzoso, Braulio ... 36

F

Failla, Osvaldo ... 34, 175, 177
Fariña, Laura ... 39
Farines, Vincent ... 87
Farkaš Vladimir ... 49
Farkas, Zoltán ... 69, 195
Fay, Justin ... 179, 202
Fekete, Gergely ... 195
Ferranti, Pasquale ... 166
Ferreira, David ... 79
Fickers, Patrick ... 169
Fischer, Gilles ... 137
Florschütz, Kristina ... 129
Foschino, Roberto ... 34, 129, 146, 166, 175, 177
Foulquié-Moreno, María R. ... 12, 143, 151
Franco, Elías ... 33
Friedrich, Anne ... 137
Fukuhara, Hideyo ... 171
Furukawa, Shuhei ... 171
G

Gaggero, Carina ... 39
Gajdoš, Peter ... 124
Galafassi, Silvia ... 146, 149
Galeote, Virginie ... 37, 79 107, 108
Gamero-Sandemetrio, Esther ... 43
Gamero, Amparo ... 106, 155
Ganga, Ma. Angélica ... 33
García-Ríos, Estéfani ... 45
García, Verónica ... 33
Gaskett, Anne C. ... 198
Gasser, Brigitte ... 27
Gay, Nicolas ... 173
Geijer, Cecilia ... 114, 189
Genheden, Samuel ... 127
Georis, Isabelle ... 131
Ghommidh, Charles ... 173
Giacomini, Alessio ... 44
Gibson, Brian ... 82, 115
Giersberg, Martin ... 129
Giorello, Facundo ... 39
Giraudo, Maria Rosa ... 25
Gobbetti, Marco ... 116
Goddard, Matthew R. ... 198
Godoy, Liliana ... 33
Gojkovic, Zoran ... 150
Gonçalves, Carla ... 165
Gonçalves, Paula ... 165
Gonzalez, Alicia ... 197
González, Beatriz ... 36
Gonzalez, James ... 197
Gonzalez, Ramon ... 102, 187
Goovaerts, Annelies ... 12
Gorban, Alexander ... 182
Gorwa-Grauslund, Marie F. ... 168

Gostinčar, Cene ... 117, 130
Gottardi, Manuela ... 80
Grabek-Lejko, Dorota ... 65
Granato, Tiziana M. ... 166
Grigoriev, Igor V. ... 117
Groenewald, Marizeth ... 23, 148
Groot Koerkamp, Marian J.A. ... 70, 195
Grube, Martin ... 117
Gschaedler, Anne ... 110, 111, 173
Guézenec, Stéphane ... 107
Guillamón, José Manuel ... 45
Gunde - Cimerman, Nina ... 25, 117, 130
Gut, Ivo G ... 136
Gutiérrez, Alicia ... 150

H

Habe, Hiroshi ... 24
Haberhauer-Troyer, Christina ... 27
Hagström, Åsa K. ... 185
Hahn-Hägerdal, Bärbel ... 29
Hähnel, Urs ... 129
Han, James ... 117
Hann, Stephan ... 27
Hansen, Jørgen ... 30
Hansen, Klavs Riishede ... 77
Hanson, Sara J. ... 196
Harashima, Satoshi ... 72
Hashida Keisuke ... 46
Hebly, Marit ... 76
Hellborg, Linda ... 185
Herrera López, Enrique ... 173
Hesselbart, Ana ... 139
Hewitt, Sarah ... 142
Höck, Heidi ... 42
Holstege, Frank C. P. ... 70, 195

Holt, Sylvester ... 12

Hrtyan, Monika ... 195

Hryniv, Orest ... 52, 60, 65

Huić Babić, Katarina ... 38

I

Iacumin, Lucilla ... 38

Iametti, Stefania ... 166

Ishchuk, Olena P. ... 175, 185

Ishii, Jun ... 172

Isobe, Tomoo ... 171

Izidoro, Iara ... 36

J

Jacobson, Dan ... 101

James, Steve ... 113

Jankowska, Dagmara ... 129

Jespersen, Lene ... 144, 153

Jeusset, Lucile ... 162

Jiménez, Ángel ... 158

Jiranek, Vladimir ... 73

Joerck-Ramberg, Dorte ... 185

Jolly, Neil ... 103

Jung, Paul ... 137

K

Kablaw, Dana ... 67

Kaboli, Saeed ... 72

Kaferle, Petra ... 179

Kalapis, Dorottya ... 195

Kalapis, Dorottya ... 69

Kaneko, Yoshinobu ... 72

Kang, Hyun Ah ... 48, 156

Kankipati, Harish Nag ... 159

Karhumaa, Kaisa ... 29

Karlsson, Emma ... 174

Kasprzak, Jakub ... 129

Kata, Iwona ... 56, 59

Katz, Michael ... 147, 150

Kavšček, Martin ... 125, 126

Kemmeren, Patrick ... 70, 195

Kida, Kenji ... 119

Kim, Hyunah ... 48

Kim, Tae Yong ... 27

Kirchmayr, Manuel ... 110

Kitamoto, Dai ... 24

Klintner, Carl Fredrik ... 29

Kočar, Nataša ... 105

Kogej, Tina ... 117

Kohlwein, Sepp D. ... 125, 126

Koike, Hideaki ... 24

Kokosar, Janez ... 175, 179

Kolecka, Anna ... 148

Köllensperger, Gunda ... 27

Kondo, Akihiko ... 118, 172

Konte, Tilen ... 47

Koppram, Rakesh ... 41, 123

Kováčová Kristina ... 49

Kovács, Károly ... 70, 195

Kovács, Mónika ... 132

Kozlov, Dmitry G ... 170

Kshanovska, Barbara ... 65

Kuanyshev, Nurzhan ... 81

Kunze, Gotthard ... 83, 129

Kurylenko, Olena ... 52, 56, 59, 61, 64

Kuzmin, Elena ... 68
L

Lamboni, Leo ... 155
Landolfo, Sara ... 133, 134
Lastauskienė, Eglė ... 160
Lazar, Viktoria ... 195
Leandro, Maria José ... 165
Lee, Dong-jik ... 48
Lee, Sang Yup ... 27
Legiša, Matic ... 163
Legras, Jean-Luc ... 37, 78, 108, 136
Leong, Su-lin L. ... 53
Leu, Jun-Yi ... 149
Lezama, Mijail ... 197
Li, Wen-Hsiung ... 11
Li, Yingying ... 12
Liccioli, Tommaso ... 73
Lindahl, Lina ... 127
Lindegaard Pedersen, Line ... 144
Linder, Tomas ... 31, 60
Ling, Zhihao ... 146, 185
Linnemann, Anita ... 155
Lionetti, Paolo ... 136
Lipzen, Anna ... 117
Lisbeth Olsson ... 174
Liti, Gianni ... 181, 182
Liu, Ping ... 202
Liu, Xin-Zhan ... 23
Llored, Agnès ... 181
Londesborough, John ... 115
López-Malo, María ... 45
López, Geovani ... 197
López, Paula ... 36
Lorca, Gustavo ... 33
Louis, Edward J. ... 182, 183
Lugo, Eugenia ... 111

Luzhetskyj, Taras ... 66
Lyzak, Oleksii ... 63

M

Maeda, Atsushi ... 122
Magalhães, Frederico ... 82
Maghradze, David ... 34, 175, 180
Makovec, Darko ... 112
Malak, Anna ... 83
Mamone, Gianfranco ... 166
Mangado, Ana ... 187
Mannazzu, Ilaria ... 108, 133, 134
Mapelli, Valeria ... 41, 42, 174
Marano, Francesca ... 75
Maráz, Anna ... 132
Marsit, Souhir ... 37
Martani, Francesca ... 75
Martí-Raga, Maria ... 188
Martin, Valentina ... 39
Martinez de la Escalera, Ximena ... 197
Martínez, Claudio ... 33
Marullo, Philippe ... 188
Marx, Christian ... 121
Mas, Albert ... 36, 188
Matallana, Emilia ... 43, 161
Mateo, Raquel Quintilla ... 12
Matsuda, Fumio ... 172
Matsuo, Shuhei ... 171
Mattanovich, Diethard ... 27
Mattiazzi Ušaj, Mojca ... 67
Mebrahtu Wisén, Sofia ... 185
Medina, Karina ... 39
Meersman, Esther ... 183
Mertens, Stijn ... 183, 184
Mezadri, Hygor ... 12
Mikkelsen, Michael Dalgaard ... 77
Minnis, David ... 140
Mirkes, Evgeny ... 182
Mita, Koichi ... 122
Mizuno, Takayuki ... 122
Moktaduzzaman, Md ... 146
Molinari, Francesco ... 166
Monerawela, Chandre ... 138
Monti, Daniela ... 166
Moon, Hye Yun ... 48
Morales, Pilar ... 102, 187
Moreno, Antonio D. ... 189
Moreno, David ... 114
Morita, Tomotake ... 24
Morrissey, John P ... 74, 85
Moses, Alan M. ... 181
Mouret, Jean-Roch ... 87
Mukherjee, Vaskar ... 12
Muñoz de las Heras, Alejandro ... 168
Mustonen, Ville ... 181
Myers, Chad L. ... 68

N

Nadai, Chiara ... 44
Nakajima, Ryoichi ... 171
Naseeb, Samina ... 140, 141
Natter, Klaus ... 125, 126, 128, 179
Newcomb, Richard D. ... 198
Ngan, Chew Yee ... 117
Nguyen Ba, Alex N. ... 181
Niba, Aziwo T. ... 53
Nicaud, Jean-Marc ... 124
Nielsen, Jens ... 86, 203

Nisiotou, Aspasia ... 35
Nitride, Chiara ... 166
Niu, Hongxing ... 169
Noble, Jessica ... 157
Nocon, Justyna ... 27
Notebaart, Richard ... 70

O

O'Keefe, Raymond ... 71
Ochangco, Honeylet Sabas ... 144
Offei, Benjamin ... 12, 143
Ogrinec, Aleš ... 105
Ohm, Robin A. ... 117
Oliveira, Bruno M. ... 13
Oliver, Stephen G ... 26
Olsson, Helen ... 127
Olsson, Lisbeth ... 41, 42, 114, 120, 121, 123, 127, 162, 189
Olstorpe, Matilda ... 53
Oom, Madalena ... 165
Oreb, Mislav ... 80
Orlić, Sandi ... 38
Orozco, Helena ... 43, 161
Ortiz-Julien, Anne ... 79, 157
Ortiz-Merino, Raúl A. ... 84
Ortmayr, Karin ... 27

P

Padilla, Beatriz ... 36
Pál, Csaba ... 69, 70, 195
Papp, Balázs ... 70, 195
Parker, Steven ... 71
Parts, Leopold ... 181
Passoth, Volkmar ... 51, 54, 58, 60

Pejo, Elia Tomas ... 114

Pelliccia, Cristina ... 175

Peña, Rubén ... 33

Pérez de los Santos, Francisco ... 158

Pérez-Torrado, Roberto ... 13, 152

Perez, Marc ... 87

Perraudin, Jean - Paul ... 131

Peter, Josephine Jasmine ... 73

Petrovič, Uroš ... 128, 175, 179

Pfeffer, Martin ... 27

Pflüg, Stefan ... 27

Picazo, Cecilia ... 43, 161

Picca Nicolino, Martina ... 183

Pickova, Jana ... 58

Piškur, Jure ... 106, 146, 147, 149, 175, 179, 185, 193, 202

Plemenitaš, Ana ... 47

Popolo, Laura ... 49

Porro, Danilo ... 81, 75

Portugal Nunes, Diogo ... 168

Pretorius, Isak S. ... 201

Preziosi-Belloy, Laurence ... 173

Prista, Catarina ... 165

Pronk, Jack T. ... 76

## Q

Querol, Amparo ... 13, 152

Quintilla, Raquel ... 148

Quirós, Manuel ... 102

## R

Radecka, Dorota ... 12, 151

Ramazzotti, Matteo ... 136

Raspor, Peter ... 105

Rauter, Marion ... 129

Ravasio, Davide ... 154

Reisser, Cyrielle ... 137

Ren, Xiao ... 155

Riechen, Jan ... 129

Riego-Ruiz, Lina ... 158

Riezman, Howard ... 127

Rivero, Damariz ... 136

Rizzetto, Lisa ... 136

Robert, Vincent ... 32

Roberts, Ian ... 113

Rojas, Virginia ... 102

Rollero, Stephanie ... 87

Romani, Luigina ... 136

Romano, Diego ... 166

Roscini, Luca ... 32, 34, 116, 175, 178

Rossouw, Debra ... 101

Roth, Frederick P. ... 34, 175

Royer, Keisha ... 148

Rozpędowska, Elżbieta ... 185

Rubio-Texeira, Marta ... 159

Ruchala, Justyna ... 52, 56, 59, 64

Ruohonen, Laura ... 82

Rußmayer, Hannes ... 27

Rutkai, Edit ... 195
S

Sablayrolles, Jean-Marie ... 87

Saels, Veerle ... 183, 184

Saint-Hubert, Catherine ... 131

Salii, Iryna ... 55

Salinas, Francisco ... 181

Salzman, Valentina ... 39

Sanchez Nogue, Violeta ... 29

Sanchez, Isabelle ... 157

Sandström, Anders G. ... 168

Santos, Aline X S ... 127

Sasano, Yu ... 46, 72

Sato, Shun ... 24

Schacherer, Joseph ... 137, 147, 181

Schifferdecker, Anna J. ... 147, 185

Semkiv, Marta ... 55, 62, 66

Sendelius, Malin ... 29

Setati, Evodia ... 175

Setati, Mathabatha E. ... 176

Sharma, Aditi ... 117

Sibirny, Andriy ... 52, 55, 56, 59, 60, 61, 62, 63, 64, 65, 66, 185

Siewers, Verena ... 86

Simpson, Jared T. ... 181

Sims, Jason ... 186

Siurkus, Juozas ... 185

Sivilotti, Paolo ... 106

Smid, Eddy ... 155

Smith, Ida M. ... 153

Smole Možina, Sonja ... 105

Snoek, Tim ... 183

Sohn, Seung Bum ... 27

Sonjak, Silva... 117

Souffriau, Ben ... 12

Stasyk, Oleh ... 52

Stasyk, Olena G. ... 50

Steensels, Jan ... 183, 184

Stefanini, Irene ... 136

Steiger, Matthias G. ... 27

Sternad Lemut, Melita ... 106

Steyn, Laurinda ... 164

Stojiljkovic, Marija ... 12

Stříbný, Jiří ... 152

Sugiyama, Minetaka ... 72

Sun, Hui ... 117

Sunada, Keisuke ... 72

Swamy, Krishna B. S. ... 149

Sybirna, Natalia O. ... 50

Sychrová, Hana ... 145

Szakács, Bianka ... 132

Szamecz, Béla ... 69, 195

T

Takagaki, Tao ... 72

Takagi, Hiroshi ... 167

Takagi, Hiroshi ... 46

Tanca, Alessandro ... 134

Tang, Yue-Qin ... 119

Tangüler, Hasan ... 109

Tassou, Chrysoula ... 35

Temitope Adeboye, Peter ... 120

Theelen, Bart ... 23

Thevelein, Johan M. ... 12, 143, 151, 159

Tilloy, Valentin ... 107

Tinta, Tinkara ... 106

Tiukova, Ievgeniia ... 54

Tkáčová, Jana ... 135

Tomás-Pejó, Elia ... 123, 162, 189

Tosato, Valentina ... 186

Trautwein, Anke ... 129
Traver, Iris ... 36

Tronchoni, Jordi ... 187

Trošt, Kajetan ... 106

Turk, Martina ... 25, 117

Tyurin, Oleg V ... 170

## U

Uršič, Katja ... 128

Ušaj, Matej ... 67, 68

Uzzau, Sergio ... 134

## V

Van Breda, Valmary ... 103

Van den Broek, Marcel ... 76

Van Haren, Luuk ... 148

Van Zeebroeck, Griet ... 159

VanderSluis, Benjamin ... 68

Varela, Javier ... 85

Verheijen, Peter J.T. ... 76

Verstrepen, Kevin ... 183, 184

Vidgren, Virve ... 82, 115

Vigentini, Ileana ... 34, 146, 166, 175, 177

Vojvoda Zeljko, Tanja ... 38, 185

Volk, Helena ... 106, 202

Vrhovsek, Urska ... 106, 150

Vu, Duong ... 32

## W

Waldron, Keith ... 113

Walker, Michelle ... 73

Walsh, Tom ... 182

Walther, Andrea ... 139, 154

Wang, Qi-Ming ... 23

Warringer, Jonas ... 181

Watanabe, Daisuke ... 46

Wendland, Jürgen ... 139, 154

Wenning, Leonie ... 86

West, Nicole ... 186

Wolfe, Kenneth H. ... 84, 196

Worch, Sebastian ... 129

Wu, Jian ... 71

## X

Xiros, Charis ... 123

## Y

Yamada, Koji ... 171

Yamakawa, Takuya ... 72

Yurkiv, Marianna ... 61

## Z, Ž

Zajc, Janja ... 117, 130

Zalar, Polona ... 25, 117

Zeef, Leo ... 140

Zemančíková, Jana ... 145

Zhao, Xinqing ... 40

Zhou, Nerve ... 106, 149

Zia, Amin ... 181

Zinkevičienė, Auksė ... 160

Žitnik, Marinka ... 67

Zupan, Blaž ... 67

Zupan, Jure ... 105
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Kmetijski inštitut Slovenije

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VIPAVA

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evolva

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VINSKA KLET
"GORIŠKA BRDA"
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