# Are stem cells from metabolically active zone of glioblastoma more likely to participate to tumor-angiogenesis via transdifferentiation?

Florent Arnauduc, Elizabeth Cohen-Jonathan Moyal, Monique

Courtade-Saidi, Solène M Evrard

► To cite this version:

Florent Arnauduc, Elizabeth Cohen-Jonathan Moyal, Monique Courtade-Saidi, Solène M Evrard. Are stem cells from metabolically active zone of glioblastoma more likely to participate to tumor-angiogenesis via transdifferentiation?. SUNRiSE (Solid Tumor Cancer Stem Cell Network), May 2024, Ajaccio, France. hal-04893267

HAL Id: hal-04893267

https://hal.science/hal-04893267v1

Submitted on 20 Jan 2025

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# Are stem cells from metabolically active zone of glioblastoma more likely to participate to tumor-angiogenesis via transdifferentiation?

Florent Arnauduc<fnref n="1" />,<fnref n="2" />, Elizabeth Cohen-Jonathan Moyal<fnref n="1" />,<fnref n="2" />,<fnref n="3" />, Monique Courtade-Saïdi<fnref n="1" />,<fnref n="2" />,<fnref n="4" />, Solène Evrard<fnref n="1" />,<fnref n="2" />,<fnref n="4" />

## INTRODUCTION

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TRANSDIFFERENTIATION

Ricci-Vitiani et al (2010) - Wang et al (2010) - Deshors et al (2019)

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TDEC

Tumor Derived Endothelial Cells

GBM
High
vascularity
Severals
mechanisms
including
transdifferentiation

STEMRI Clinical Trial
Spectroscopy MRI (MRSI)

Lemarié et al (2023)

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Tom Maillet' Talk
Anthony Lemarié'
Poster

Metabolic Active Zone
CNI+

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Enriched area in
GSC neurospheres
CNI+

Metabolic Non Active Zone
CNI-

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Poor area in GSC
neurospheres
CNI-

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## Do GSCs from the active metabolic zone (CNI+) have more abilities to transdifferentiate into TDEC?

## METHODS

GSCs from 4 different patients

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CNI+

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GSC

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TDEC

Lineage with cells from the
metabolic active zone and
non-active zone

GBM
CNI+ or CNI-

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CNI-

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GSC

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TDEC

## Analysis:

Endothelial functional characteristics

Pseudotube Formation: Quantification of TDEC by total measurements in µm

Endothelial phenotypic characteristics

Endothelial Markers vs Stem cells Markers:
mRNA and Protein Expression
Signaling Pathways involved

## RESULTS

Endothelial functional characteristics

<figure><img src="image_27.png" /><figcaption>Pictures of pseudotubes obtained X10</figcaption></figure>

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<figure><img src="image_29.png" /><figcaption>HUVEC
(Endothelial Cell Control)</figcaption></figure>

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TDEC obtained from active metabolic zone GSCs form more pseudotubes in Matrigel®

## RESULTS

Endothelial phenotypic characteristics

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<figure><img src="image_35.png" /><figcaption>Signaling pathway involved</figcaption></figure>

Preliminary results: Only Cell Line 1

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VEGFR2/ERK pathway seems to be activated in TDEC obtained from active metabolic zone

TDEC from cell Line 1: Acquisition of CD31 and loss of Sox2

TDEC from cell Line 2: Loss of Sox2 but absence of CD31 expression

TDEC from cell Line 3 (n=2): Variable expression of Sox2 and absence of CD31 expression

## CONCLUSION

TDECs obtained from active metabolic zone GSCs show an increased capacity to form pseudotubes although this depends on the patient

The phenotypic characteristics of TDECs also depends on the patient and additional experiments are necessary

The signaling pathways involved are still being explored : our preliminary results on one cell line show that VEGFR2/ERK pathway is activated

<footnote n="1">¹INSERM U1037, CRCT: Cancer Research Center of Toulouse, France,</footnote>

<footnote n="2">²Faculté de Santé Université Toulouse III, France,</footnote>

<footnote n="3">³Service de radiothérapie, IUCT Oncopole, Toulouse, France,</footnote>

<footnote n="4">⁴Département ACP, CHU Toulouse, France</footnote>