# Mechanisms and impact of ribosomal RNA methylation in plants

Sara Alina Neumann, Jacinthe Azevedo-Favory, Rémy Merret, Marie-Christine Carpentier, Julien Henry, V. Marchand, Anne Debures, Magali Rompais, Christine Carapito, Yuri Motorin, et al.

<figure><img src="image_1.png" /></figure>

## To cite this version:

Sara Alina Neumann, Jacinthe Azevedo-Favory, Rémy Merret, Marie-Christine Carpentier, Julien Henry, et al.. Mechanisms and impact of ribosomal RNA methylation in plants. The 2024 Post-transcriptional Gene Regulation in Plants meeting, Sep 2024, Banyuls-Sur-Mer, France. . hal-04841568

HAL Id: hal-04841568

https://hal.science/hal-04841568v1

Submitted on 16 Dec 2024

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers.

L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

<figure><img src="image_2.png" /></figure>

Distributed under a Creative Commons Attribution 4.0 International License
Mechanisms and impact of ribosomal RNA
methylation in plants

Sara Alina Neumann * <fnref n="1" />, Jacinthe Azevedo-Favory <fnref n="2" />, Remy Merret <fnref n="2" />,
Marie-Christine Carpentier <fnref n="2" />, Julien Henry <fnref n="3" />, Virginie Marchand <fnref n="4" />, Anne
De Bures <fnref n="2" />, Magali Rompais <fnref n="5" />, Christine Carapito <fnref n="5" />, Christine Gaspin <fnref n="3" />,
Yuri Motorin <fnref n="6" />, Julio Saez-Vasquez† <fnref n="2" />

Ribose methylation (2-O-Me) is the most common modification of ribosomal RNAs (rRNA) in various life forms and are pivotal for ribosome assembly and translation. These 2-O-Me modifications are guided by small nucleolar C/D-box RNAs (C/D snoRNA) and are deposited by the methyltransferase FIBRILLARIN (FIB). Our work, performed in the plant model *Arabidopsis thaliana*, highlights that rRNA 2-O-Me is a dynamic process. To understand the impact of such modulation of rRNA 2-O-Me on translation, we examined Arabidopsis plants lacking the major nucleolar protein Nucleolin 1 (NUC1). Depletion of NUC1 (*nuc1*) resulted in decreased rRNA 2-O-Me levels(1) and, hence, in ribosome hypomethylation. Our findings suggest that reduced rRNA 2-O-Me in *nuc1* may be triggered by defects in the assembly of the C/D snoRNP complex. Ribosome hypomethylation leads to an accumulation of polysomes detectable in their polysome profiles and a stalling of ribosomes at the start codon shown in 5'Pseq analysis. We are now trying to elucidate if this stalling could be either inferred to the formation of the 80S complex or to difficulties in interacting with translation initiation factors. All in all, our results underscore the crucial role of rRNA methylation and the heterogeneity of ribosomes in their assembly and translation processes. (1) Azevedo-Favory J et al. (2021) RNA Biology 18:11, 1-18

**Keywords:** ribosomal RNA, Ribosome methylation, Ribosomes

<footnote n="1">¹ LGDP UMR5096, Perpignan, France – Laboratoire Génome et Développement des Plantes – France</footnote>

<footnote n="2">² LGDP UMR5096, Perpignan, France – Laboratoire Génome et Développement des Plantes – France</footnote>

<footnote n="3">³ Université Fédérale de Toulouse, INRAE, MIAT, 31326, Castanet Tolosan, France – MIAT, University of Toulouse, INRA – France</footnote>

<footnote n="4">⁴ Université de Lorraine, CNRS, INSERM, IBSLor, (UMS2008/US40), Epitranscriptomics and RNA Se-quencing (EpiRNA Seq) Core Facility, F 54000 Nancy – CNRS, INSERM, IBSLor, (UMS2008/US40), Epitranscriptomics and RNA Sequencing (EpiRNA-Seq) Core Facility – France</footnote>

<footnote n="5">⁵ Laboratoire de Spectrométrie de Masse BioOrganique, Institut Pluridisciplinaire Hubert Curien, UMR7178 CNRS/Université de Strasbourg, Strasbourg, France – CNRS UMR7178 – France</footnote>

<footnote n="6">⁶ Université de Lorraine, CNRS, INSERM, IBSLor, (UMS2008/US40), Epitranscriptomics and RNA Se-quencing (EpiRNA Seq) Core Facility, F 54000 Nancy, France – CNRS, INSERM, IBSLor, (UMS2008/US40), Epitranscriptomics and RNA Sequencing (EpiRNA-Seq) Core Facility – France</footnote>

<footnote>*Speaker</footnote>

<footnote>†Corresponding author: saez@univ-perp.fr</footnote>