# Lateral gene transfers have polished animal genomes: lessons from nematodes

Etienne Danchin, Marie-Noelle Rosso

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Etienne Danchin, Marie-Noelle Rosso. Lateral gene transfers have polished animal genomes: lessons from nematodes. Frontiers in Cellular and Infection Microbiology, 2012, 2 (27), pp.1-5. 10.3389/fcimb.2012.00027. hal-01267787

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# Lateral gene transfers have polished animal genomes: lessons from nematodes

Etienne G. J. Danchin<fnref n="1" />,<fnref n="2" />,<fnref n="3" />* and Marie-Noëlle Rosso<fnref n="1" />,<fnref n="2" />,<fnref n="3" />†

**Edited by:**

Didier Raoult, Université de la Méditerranée, France

**Reviewed by:**

Kalliopi Georgiades, Unité de Recherche en maladies infectieuses tropicales emergentes, France
Pierre Pontarotti, Centre National de la Recherche Scientifique, France

*Correspondence:

Etienne G. J. Danchin, Institut Sophia Agrobiotech, INRA-CNRS-UNSA, 400 route des Chappes, F-06903 Sophia-Antipolis, France.
e-mail: etienne.danchin@sophia.inra.fr

†Present address:

INRA, Université Aix-Marseille, UMR1163 Biotechnologie des Champignons Filamenteux, F-13288 Marseille, France.

It is now accepted that lateral gene transfers (LGT), have significantly contributed to the composition of bacterial genomes. The amplitude of the phenomenon is considered so high in prokaryotes that it challenges the traditional view of a binary hierarchical tree of life to correctly represent the evolutionary history of species. Given the plethora of transfers between prokaryotes, it is currently impossible to infer the last common ancestral gene set for any extant species. For this ensemble of reasons, it has been proposed that the Darwinian binary tree of life may be inappropriate to correctly reflect the actual relations between species, at least in prokaryotes. In contrast, the contribution of LGT to the composition of animal genomes is less documented. In the light of recent analyses that reported series of LGT events in nematodes, we discuss the importance of this phenomenon in the evolutionary history and in the current composition of an animal genome. Far from being neutral, it appears that besides having contributed to nematode genome contents, LGT have favored the emergence of important traits such as plant-parasitism.

Keywords: lateral gene transfer, horizontal gene transfer, genome, nematode, eukaryote, adaptation, evolution

## INTRODUCTION

Lateral gene transfer (LGT), the transmission of genetic material from one species to another by means other than direct inheritance from parents to the offspring is a prevalent phenomenon in prokaryotes. LGT contribution to the composition of today bacterial genomes is so high that it challenges the idea that genome evolution in these species follows a tree-like pattern. Besides contributing to the composition of their genomes LGT events also play significant role in the acquisition of important biological traits. For instance, antibiotic resistance is frequently acquired during the lifespan of a bacterium and transmitted to its offspring rather than inherited from an ancient ancestor. Whether LGT events have significantly contributed to the composition of metazoan genomes and whether they are involved in the acquisition of new traits remains poorly documented, in contrast. This is probably in part because LGT in these species appears conceptually and mechanistically less evident than for prokaryotes. Here, we discuss an ensemble of LGT cases reported in different nematode lineages with different lifestyles, including necromenic, plant-parasitic, and animal-parasitic species. Although it is currently difficult to evaluate the contribution of LGT to the composition of nematode genomes, in the lack of a comprehensive whole genome scan, we can already assume that LGT have probably played important role in the acquisition of some traits such as plant-parasitism.

## LGT IN NECROMENIC NEMATODES

The necromenic nematode *Pristionchus pacificus* spends a considerable part of its life cycle associated with beetles and is supposed to feed on bacteria and fungi that decompose the insect once it is dead. The genome sequencing of *P. pacificus* revealed the presence of functional cellulase genes acquired via LGT of microbial origin (Dieterich et al., 2008). A more detailed analysis of these cellulase genes in the *Pristionchus* genus showed that the genes were probably acquired ancestrally as different *Pristionchus* species possessed these genes and their phylogeny matched the species phylogeny (Mayer et al., 2011). The same analysis also showed that these genes underwent high rates of duplications and losses suggesting that the number of cellulase genes in *Pristionchus* species is under selection. Interestingly, the genome of *P. pacificus* also revealed the presence of Diapausin genes otherwise absent from nematodes but present in beetles. A phylogenetic analysis coupled with multi-species comparison of codon usage bias, revealed that Diapausin genes as well as ca. 500 others in the *P. pacificus* genome might have been acquired via LGT of insect origin (Rodelsperger and Sommer, 2011). Most of the putatively transferred genes of insect origin were retrotransposons, and it is hypothesized that these mobile elements might have played themselves a role of vector in the transfer of other genes. These first clear examples of putative transfers between two metazoan animals indicate that our current view of the amplitude of the LGT phenomenon in

<footnote n="1">¹ Institut National de la Recherche Agronomique, UMR 1355 ISA, 400 route des Chappes, Sophia-Antipolis, France</footnote>

<footnote n="2">² Centre National de la Recherche Scientifique, UMR 7254 ISA, 400 route des Chappes, Sophia-Antipolis, France</footnote>

<footnote n="3">³ Université de Nice-Sophia Antipolis, UMR ISA, 400 route des Chappes, Sophia-Antipolis, France</footnote>
animals might be underestimated because most analyzes have
been so far restricted to transfers of non-metazoan origin.

## LGT IN ANIMAL-PARASITIC NEMATODES

The mutualistic symbiosis between alpha-proteobacteria Wolbachia and Onchocercids has for long stimulated scientific interest due to the possibility to control these causing agents of severe human diseases, such as river blindness and elephantiasis by antibacterial treatments. The interdependence between the symbiont and its host seems to involve the supply by the nematode of amino acids required for Wolbachia growth and the ability of the symbiont to complete the synthesis of compounds crucial to the nematode such as heme, riboflavin and nucleotides (Foster et al., 2005; Ghedin et al., 2007). Several LGTs from endosymbiont to the nematode genome have been described (Fenn et al., 2006; Dunning Hotopp et al., 2007; McNulty et al., 2010) and may have been facilitated by the close association of the bacteria to germline cells in the female stage (Ferri et al., 2011; Fischer et al., 2011). Until recently, it was generally assumed that the presence of the bacterial symbiont in Onchocercids could result from a single colonization event in an ancestor of the lineage and that the bacteria was secondarily lost in a few nematode species able to proliferate free from bacteria. The presence of transcriptionally active bacterial genes in the genomes of two distantly related Wolbachia-free Onchocercids species indicates that these species might have acquired bacterial genes before symbiont loss (McNulty et al., 2010). However, evidence for a recent Wolbachia capture in an Onchocercid species associated with the Japanese black bear modifies the current appraisal on the co-evolution of the endosymbiont and its host (Ferri et al., 2011). In addition, a screen for the presence of the bacteria in yet unexplored genera and species identified 63% of Onchocercids devoid of Wolbachia, notably the ancestral Oswaldofilariinae (Ferri et al., 2011). It will be most interesting to screen the genomes of these bacteria-free nematodes to search for traces of LGT and assess whether they lost their symbiont after acquisition of bacterial genes.

## LGT IN PLANT-PARASITIC NEMATODES

The ability to feed on plants arose at least three times inde-
pendently in the phylum Nematoda (Blaxter et al., 1998). How
nematodes evolved toward plant-parasitism is a fascinating ques-
tion. All phytonematodes feed from the cytoplasm of living plant
cells. Ectoparasites perforate plant tissues with a protrusable stylet
that reaches the cell layers they feed on whereas endoparasites
penetrate plant tissues. Both ecto- and endoparasites, therefore,
require active degradation or softening of the physical barrier
formed by plant cell walls. The endoparasitic cyst and root-
knot nematodes are among the most notorious phytonematodes,
causing major damages to crops worldwide. The identification
in cyst nematodes of genes encoding cellulases most similar to
bacterial enzymes paved the way for the assumption that LGT
could have participated in adaptation to plant-parasitism (Smant
et al., 1998). Since then, several nematode genes encoding cell
wall-degrading or -modifying enzymes were presented as can-
didates for LGT (Davis et al., 2000). The analysis of root-knot
nematode genomes revealed an unprecedented repertoire of cell

wall-degrading and -modifying enzymes in an animal that cov-
ered six different protein families able to degrade all major
polysaccharides of cell walls, i.e., cellulose, hemicellulose and
pectin (Abad et al., 2008; Opperman et al., 2008). Homologs
for these nematode cell wall-degrading enzymes were searched in
public databases, checked for significance and used for systematic
robust phylogenetic reconstruction (Danchin et al., 2010). Two
noticeable findings from this analysis were that those genes most
likely originated from several independent LGT events and that
in most cases the closest relatives were found in bacteria from the
rhizosphere. Indeed, LGT events have been traced back at differ-
ent nodes of the nematoda phylogeny. Some LGT events appear so
far lineage-specific, for instance, GH28 polygalacturonases were
only found to date in root-knot nematodes. In contrast, other
genes such as those encoding GH5 cellulases or PL3 pectate
lyases are found in multiple plant-parasitic nematode lineages,
suggesting a more ancient acquisition in a common ancestor.
Interestingly, several different bacterial species appear as poten-
tial donors for the different families of plant cell wall-degrading
enzymes. For example, the closest relatives of root-knot nematode
GH28s are found in *Ralstonia solanacearum*, a plant-pathogenic
soil bacterium that shares plant hosts with these nematodes. On
the other hand, nematode PL3 pectate lyases cluster with PL3s
of *Clavibacter michiganensis*, another plant pathogen evolution-
ary distant from *Ralstonia solanacearum*. Overall, this analysis
suggests that the ecology of the donor is probably more impor-
tant than its phylogenetic position for a gene transfer to occur.
This is consistent with similar conclusions drawn from the anal-
ysis of LGT events between bacteria of the human microbiome
(Smillie et al., 2011). Another interesting feature of genes encod-
ing cell wall-degrading enzymes in nematodes is their frequent
organization in large multigene families. As many as 30 pectate
lyases, 21 cellulases and 20 expansin-like genes were identified
in the genome of *M. incognita* (Abad et al., 2008) and similar
gene family expansions were observed in the genome of *M. hapla*
(Opperman et al., 2008). Gene abundance in these families sug-
gest that gene duplications have been under positive selection
during the evolution of these plant-parasites.

Several experimental data further indicate that LGT could
have participated to adaptation to plant-parasitism. First, tran-
scripts for all cell wall-degrading enzymes have been localized
in the esophageal glands of the nematodes (Rosso et al., 2012).
These oesophageal glands are specialized cells in which proteins
secreted during infection are produced. In line with this find-
ing, the systematic presence of a secretion signal peptide on the
predicted protein sequences suggested a role for the enzymes out-
side the nematode during parasitism. Second, cellulases, pectate
lyases, polygalacturonases, and expansin-like proteins were enzy-
matically active, although examples of potential pseudogenization
were identified in migratory nematodes (Bera-Maillet et al., 2000;
Popeijus et al., 2000; Jaubert et al., 2002; Qin et al., 2004; Mitreva-
Dautova et al., 2006; Haegeman et al., 2010). Immunolocalization
studies showed the secretion of cellulases and pectate lyases *in
planta* during migration of the juveniles and comforted a role
in cell wall degradation or cell wall softening during host inva-
sion (Wang et al., 1999; Doyle and Lambert, 2002; Vieira et al.,
2011). Moreover, immunolocalizations in root-knot nematode
females provided a hint for an additional role of cellulases during egg laying at the surface of the root (Vieira et al., 2011). Finally, the reduced virulence of nematodes after knock-down of cellulase genes established the role of these enzymes in the success of infection (reviewed in Rehman et al., 2009; Rosso et al., 2009; Haegeman et al., 2009a).

Besides the clear examples of genes encoding cell wall-modifying enzymes, a series of other genes have been acquired by LGT in phytonematodes and also probably contributed to the emergence and success of plant-parasitism in these species (reviewed in Haegeman et al., 2011). The processes in which the gene products are putatively involved include crucial mechanisms of the parasitic interaction, such as establishment of the nematode's feeding structure, modulation of plant defense pathways and processing of nutrients absorbed from the plant. As for genes encoding cell wall-degrading enzymes, the principal putative donor species for these genes are soil bacteria but for a few other genes fungal species appear as the most likely donors.

## CONCLUDING REMARKS

This bunch of indications for LGT occurrences in nematodes adds to other recent findings on LGT in eukaryotes and modifies our viewpoint on the prevalence of gene transfers in animals (for reviews see Keeling and Palmer, 2008; Dunning Hotopp, 2011). In plant parasitic nematodes, several LGT events occurred at different time points of their evolution. In addition, the transferred genes seem to originate from different donor organisms. However, any conclusion on the multiplicity of potential donors should be considered with caution as our view on gene transfers frequency between prokaryotes is evolving rapidly. Identification of the exact donor bacteria may be complicated by several aspects. The true donor bacteria might not exist anymore or might not have been characterized at the sequence level. In these cases the next bacteria sharing the highest similarity with the studied gene might be considered as donor by default. Also, due to the high frequency of transfers between bacteria, we cannot exclude that the gene transferred from a bacterium to a nematode had itself been already transferred before from another bacteria. Indeed, genes transferred multiple times have been described in proteobacteria (Kloesges et al., 2011). The observed transfer might thus be a secondary event.

How could foreign genes be integrated into the genome of nematodes is certainly the main black box here. Gene transfers from the endosymbiont *Wolbachia* in Onchocercids can be facilitated by the presence of the bacteria in germ cells, though the exact genetic mechanism of transfer is unknown.

Noticeably, no symbiont has been identified so far in the root-knot and cyst nematodes mentioned above. Still, ancestors of phytonematodes could have held bacterial symbionts, as suggested by the presence of an endosymbiotic bacterium from the *Wolbachia* supergroup within the reproductive tract of *Radopholus similis* females, plant-parasitic nematodes that share a common ancestor with cyst- and root-knot nematodes (Haegeman et al., 2009b). However, in the case of genes encoding plant cell wall-degrading enzymes, an origin from an ancient Wolbachia-like symbiont appears highly unlikely because in no phylogenetic analysis was a

Wolbachia or Wolbachia-like sequence identified as an homolog (Danchin et al., 2010).

The two insect parasitic nematodes Steinernema and Heterorhabditis provide another example of close association between nematodes and bacteria. Gamma-proteobacteria dwell in the gut of infective juveniles of these two nematodes and in rectal glands of Heterorhabditis females (Ciche and Ensign, 2003). The genome of these nematodes is not yet available and if new cases of LGT of bacterial origin were identified here, they could indicate the possibility of transfer to the germline cells without direct contact with the bacteria.

An alternative hypothesis for the origin of LGT events is the "you are what you eat" hypothesis first formulated in (Doolittle, 1998). In root-knot nematodes, four different groups of bacteria can be viewed as potential donors of plant cell wall-degrading enzyme genes. Interestingly, three of these groups include notorious plant pathogens or symbionts having interactions within plant hosts and thereby sympatric with these nematodes (Danchin et al., 2010). One possibility is that an ancestor of plant-parasitic nematodes was initially bacterivorous and used to feed on soil bacteria, including plant-pathogenic bacteria (Figure 1). By mechanisms discussed later, genetic material from digested bacteria might have been transferred to the nuclear genome of nematodes. In the case of cell wall-degrading enzymes,

<figure><img src="image_3.png" /><figcaption>**FIGURE 1 | An hypothesis for lateral gene transfer from bacteria to plant-parasitic nematodes.** LGT could have been favored in ancestral nematodes that shared their ecological niche with plant-associated bacteria in soil (a), in the rhizosphere (b) or in bacteria-infected plant tissues (c).</figcaption></figure>
the transferred genes likely provided the nematode the ability to penetrate plant tissue and in a first time to access root-dwelling bacteria otherwise inaccessible to other bacterivorous nematodes. This selective advantage might have favored individuals that possessed bacterial genes encoding cell wall-degrading enzymes and following a LGT ratchet mechanism, individuals with additional enzymes acquired via LGT might have been positively selected generation after generation and eventually developed a plant-parasitic lifestyle.

Whatever the origin, from ancient endosymbionts or from feeding, different potential mechanisms of transfer can be envisioned. They may involve bacterial secretion systems, bacteriophages, plasmids, or viruses as well as passive or active spread of DNA fragments from digested bacterial cells (Danchin, 2011). Transposable elements can also be considered as potential vectors, particularly in the case of Pristionchus in which retrotransposons have been found associated with transferred genes. In contrast, no evidence for a proximity of transposable elements in the vicinity of transferred genes in plant-parasitic nematodes has been described.

Overall, it appears that LGT, both from bacterial and fungal origins have not only contributed to the current composition of nematode gene repertoires but also had crucial importance in the emergence of new biological capabilities. Although several examples of LGT of non-metazoan origin in nematode genomes have been reported, the total contribution of LGT to the composition of a nematode genome is yet poorly described. To date,

no comprehensive list of genes putatively acquired via LGT in a nematode genome has been published. It is also largely unknown whether transfers of animal or plant origin substantially contributed to the composition of nematode genomes. The recent example of transfers from insects to necromenic nematodes suggests this might actually be the case.

The idea that a Darwinian binary tree-like representation incorrectly reflects the evolutionary history of genes and genomes has been raised as soon as 1975 (Sneath, 1975). Based on the observation that LGT occur frequently in bacteria and that reticulate evolution, another phenomenon challenging binary tree-like representation is common in flowering plants, Sneath already proposed that a network-like representation might be more accurate. This idea has found echoes more recently in the light of whole genome analysis, suggesting that, at least in bacteria, evolution more resembles a rhizome than a bifurcating tree (Raoult, 2010). We cannot state at the moment to what extent could LGT events disturb a binary tree-like representation in the phylum nematoda. Regardless the potential contribution of LGT to the composition of nematode genomes, it appears clear, at least in the case of plant-parasitic nematodes that these events have contributed to adaptation to a new life style.

## ACKNOWLEDGMENTS

This work benefited from links funded through European COST Action 872.

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**Conflict of Interest Statement:** The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Received: 23 January 2012; paper pending published: 14 February 2012; accepted: 21 February 2012; published online: 06 March 2012.

Citation: Danchin EGJ and Rosso M-N (2012) *Lateral gene transfers have polished animal genomes: lessons from nematodes*. *Front. Cell. Inf. Microbio.* 2:27. doi: 10.3389/fcimb.2012.00027

Copyright © 2012 Danchin and Rosso. This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.