# A novel grapevine color locus regulates anthocyanin pigmentation of vegetative organs in response to UV-B

Agnes Ageorges

► To cite this version:

Agnes Ageorges. A novel grapevine color locus regulates anthocyanin pigmentation of vegetative organs in response to UV-B. 8. International Workshop on Anthocyanins, Sep 2015, Montpellier, France. hal-01602107

HAL Id: hal-01602107

https://hal.science/hal-01602107

Submitted on 3 Jun 2020

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# The 8th International Workshop on Anthocyanins

Montpellier (France)

16-18/09/2015

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Thursday September 17th

09h00 - 11h40 SESSION 1- Biosynthesis and Genetics

Chairs: Dr. Kevin DAVIES & Dr. Nicole HUGHES

**09h00-** New plant breeding techniques for bio-sustainable production of natural colors in black carrot.

H. BRINCH-PEDERSEN and I. B. HOLME

**09h20-** Multiple copies of a simple MYB-binding site confer trans-regulation by flavonoid-related R2R3 MYBs.

R. ESPLEY, C BRENDOLISE, C. BUTTS, K. LIN-WANG, T. MCGHIE, C. BAVA, S. TOMES, R. HELLENS, and A. ALLAN

**09h40-** Evidence of common genetic control of anthocyanin and flavonol content and composition in mature grapes.

G. MALACARNE, S. LORENZI, E. COLLER, J. BATTILANA, M. TROGGIO, U. VRHOVSEK, F. MATTIVI, M. GRANDO, C. MOSER, and L. COSTANTINI

**10h00-** Purple yeast: reconstruction of anthocyanin pathway in yeasts
bottlenecks and challenges.

S. MARTENS

10h20 - 11h00 Coffee break

Chairs: Dr. Richard ESPLEY & Dr. Nobuhiro SASAKI

**11h00-** A novel grapevine color locus regulates anthocyanin
pigmentation of vegetative organs in response to UV-B.

J.T. MATUS, E. CAVALLINI, R. LOYOLA, J. HÖLL, L. FINEZZO,
J. BOGS, G.B. TORNIELLI, A. AGEORGES, and P. ARCE-
JOHNSON

**11h20-** *In planta* anthocyanin degradation by a vacuolar class III peroxidase in *Brunfelsia calycina* flowers.

G. ZIPOR, P. DUARTE, I. CARQUEIJEIRO, R. OVADIA, P. TEPER-BAMNOLKER, D. ESHEL, Y. LEVIN, A. DORON-FAIGENBOIM, M. SOTTOMAYOR, and M. OREN-SHAMIR
A novel grapevine color locus regulates anthocyanin pigmentation of vegetative
organs in response to UV-B

José Tomás Matus<fnref n="1" />*, Erika Cavallini<fnref n="2" />, Rodrigo Loyola<fnref n="3" />,<fnref n="4" />, Janine Höll<fnref n="5" />, Laura Finezzo<fnref n="2" />, Jochen Bogs<fnref n="6" />, Giovanni Battista Tornielli<fnref n="2" />, Agnès Ageorges<fnref n="7" />, Patricio Arce-Johnson P<fnref n="4" />

The color of grapevine berries relies on the allelic condition of a major locus on chromosome 2 harboring at least two functional R2R3-MYB genes; *MYBA1* and *MYBA2*. These transcription factors control different steps of the anthocyanin-specific branch within the phenylpropanoid pathway. The simultaneous occurrence of transposon insertions, deletions and/or point mutations in these genes are responsible for the un-pigmented phenotype of most white-skinned grapes but red pigmentation found in vegetative organs of these cultivars suggests the presence of additional regulators. We previously performed a genome-wide analysis of the R2R3-MYB family in grapevine showing that those genes related to flavonoid synthesis had expanded in number. These tandem and segmental duplication events were specific to the anthocyanin, proanthocyanidin (PA) and C2 motif repressor clades, but excluded other closely related groups such as the flavonol or thricome clades. The following presentation describes a cluster in chromosome 14 containing three *MYBA*-closely related genes. Their ectopic expression in grapevine, followed by transcriptomic analysis showed that these genes activate several flavonoid genes promoting the specific accumulation of anthocyanins. Transient gene reporter assays showed they share many targets with *MYBA1/A2*, but largely differ in activating the synthesis of tri-hydroxylated anthocyanins. In contrast to *MYBA1*, these genes seem to form part of the intrinsic response of grapevine to UV-B radiation. These results demonstrate the involvement of two distinct loci in a tissue-specific regulation of anthocyanin synthesis in grapevine.

Keywords: grape, ultraviolet radiation, leaves, MYB.

<footnote n="1">¹ Center for Research in Agricultural Genomics, (CSIC-IRTA- UAB-UB), Barcelona 08034, Spain</footnote>

<footnote n="2">² Department of Biotechnology, University of Verona, 15-37134 Verona, Italy</footnote>

<footnote n="3">³ Departamento de Genética Molecular y Microbiología, Pontificia Universidad Católica de Chile,
8331150 Santiago, Chile</footnote>

<footnote n="4">⁴ Departamento de Fruticultura y Enología, Pontificia Universidad Católica de Chile, 6904411, Santiago, Chile</footnote>

<footnote n="5">⁵ Centre for Organismal Studies Heidelberg, University of Heidelberg, 69120 Heidelberg, Germany</footnote>

<footnote n="6">⁶ Weincampus Neustadt, DLR Rheinpfalz, 67435 Neustadt, Germany</footnote>

<footnote n="7">⁷ INRA, UMR 1083 SPO, F-34060 Montpellier, France</footnote>

<footnote>+ Corresponding authors: giovannibattista.tornielli@univr.it; ageorges@supagro.inra.fr;
parce@bio.puc.cl</footnote>

<footnote>*Email: tomas.matus@gmail.com</footnote>