# Intermittent fasting protects the heart following acute stress induced by an ischemia-reperfusion episode in healthy rats

Alexis Jouenne, Frank Kober, Monique Bernard, Martine Desrois, Joevin Sourdon

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## To cite this version:

Alexis Jouenne, Frank Kober, Monique Bernard, Martine Desrois, Joevin Sourdon. Intermittent fasting protects the heart following acute stress induced by an ischemia-reperfusion episode in healthy rats. Printemps de la cardiologie 2025, May 2025, Saint-Malo, France. 118 (6-7), pp.S192, 2025, 10.1016/j.acvd.2025.03.043. hal-05108226

HAL Id: hal-05108226

https://hal.science/hal-05108226v1

Submitted on 23 Jul 2025

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# Intermittent fasting protects the heart following acute stress induced by an ischemia-reperfusion episode in healthy rats

Alexis Jouenne, Frank Kober, Monique Bernard, Martine Desrois, Joevin Sourdon

Aix-Marseille Université, CNRS UMR 7339, Marseille

## Introduction

Intermittent fasting (IF) → Food deprivation ~ 16h in humans (24h in rodents)

* Slows aging and protects against neurodegenerative diseases

* Prevents metabolic syndrome in rodents and humans

* Anti-inflammatory and anti-apoptosis

* Preclinical: prevents post-myocardial infarction remodeling

## Objectives

Does IF confer **cardioprotection** involving improvement in **energy metabolism** during acute stress induced by **ischemia-reperfusion**?

References: Longo, VD & Mattson, MP. (2014). Cell Metab; Wan, R et al. (2003). FASEB J; Ahmet, I et al. (2005). Circulation; Hammer, S et al. (2008). J Clin Endocrinol Metab

## Materials & Methods

### Female Wistar rats

IF: fed 1 day out of 2 for 4 months (n=6)

CTL: ad libitum for 4 months (n=6)

* Intraepitoneal glucose tolerance test

* *In vivo* cardiac MRI (PharmaScan 7T)

* *Ex vivo* ³¹P MRS on isolated heart (AM) at rest, during ischemia and at reperfusion

## Results: IPGTT

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## Results: MRI and cardiac function

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## Results: ischemia-reperfusion and ³¹P-MRS

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## Conclusion

* Improved carbohydrate homeostasis

* Recovery of cardiac function after 32 minutes of ischemia

* Optimal maintenance of energy metabolism → ATP

## Perspectives

### Does IF protective :

* Through reduced oxidative stress?

* In a pathological context (pre-diabetes, type 2 diabetes, ...)?