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# Mites of the genus Oulenziella Fan and Zhang (Acari: Winterschmidtiidae), with description of a new species from Kenya

Qing-Hai Fan, Farid Faraji

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Qing-Hai Fan, Farid Faraji. Mites of the genus Oulenziella Fan and Zhang (Acari: Winterschmidtiidae), with description of a new species from Kenya. Acarologia, 2022, 62 (1), pp.148-160. 10.24349/i2bo-zeic. hal-03552254

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# Mites of the genus *Oulenziella* Fan & Zhang (Acari: Winterschmidtiidae), with description of a new species from Kenya

Qing-Hai Fan IDª, Farid Faraji IDᵇ,ᶜ

## Original research

### ABSTRACT

The genus *Oulenziella* Fan and Zhang contains two species, *O. bakeri* (Hughes) and *O. longiseta* Barbosa and Moraes. This paper describes a third species, *O. africana* sp. n., collected from mango trees in Kenya, and provides a new record of *O. bakeri* for this country. We redefine the genus *Oulenziella* based on new data and provide an updated key to genera of the subfamily Oulenziinae (Acari: Astigmata: Winterschmidtiidae) and a key to species of the genus *Oulenziella*.

**Keywords** Sarcoptiformes; Astigmata; Kenya; Oulenziinae; new species; new record

**Zoobank** http://zoobank.org/1FF85B7C-B11A-4E45-A6D7-AC2D921B3196

## Introduction

The genus *Oulenziella* was established by Fan and Zhang in 2015 based on *Calvolia bakeri* Hughes collected from India (Fan *et al.* 2015). It remained monotypic until the recent publication of *Oulenziella longiseta* Barbosa and Moraes, collected from the states of Mato Grosso and Bahia, Brazil (Barbosa & Moraes 2021). Both species are arboreal in tropical and subtropical areas (Fan *et al.* 2020; Barbosa & Moraes 2021). Because it is arboreal *O. bakeri* was considered a potential alternative food for biological control agents such as species in the family Phytoseiidae. *Oulenziella bakeri* is fungivorous (Fan *et al.* 2015) and has been successfully cultured in the laboratory on yeast (Liu & Zhang 2016) and mass reared on substrate containing yeast or a combination of yeast, flour, wheat bran, vermiculite and sawdust for the commercial production of *Neoseiulus californicus* (McGregor) (Phytoseiidae) in China (Jiang 2014; Zhu *et al.* 2019) for control of the two spotted spider mite, *Tetranychus urticae* (Koch) (Tetranychidae).

The life cycle of *Oulenziella bakeri* consists of five stages: egg, larva, protonymph, tritonymph and adult (no heteromorphic deutonymph observed). Fan *et al.* (2020) studied all postembryonic stages of *O. bakeri* except the deutonymph and provided ontogenetic data useful in understanding structural homologies, making taxonomic decisions, and reconstructing phylogenies. The present study proposes a third species of *Oulenziella* collected from mango trees in Kenya, provides a record of *O. bakeri* from Kenya, redefines the genus, and provides a diagnostic key to species of *Oulenziella* as well as an updated key to genera of the subfamily Oulenziinae.

Received 12 July 2021
Accepted 28 January 2022
Published 02 February 2022

Corresponding author
Qing-Hai Fan ID:
qinghai.fan@mpi.govt.nz

Academic editor
Auger, Philippe

https://doi.org/
10.24349/i2bo-zeic

ISSN 0044-586X (print)
ISSN 2107-7207 (electronic)

<figure><img src="image_8.png" /></figure>

Fan Q. and Faraji F.

Licensed under
Creative Commons CC-BY 4.0

<footnote>ª Plant Health and Environment Laboratory, Ministry for Primary Industries, Auckland, New Zealand.</footnote>

<footnote>ᵇ Eurofins MITOX BV, Science Park 408, 1098 XH Amsterdam, The Netherlands.</footnote>

<footnote>ᶜ Institute for Biodiversity and Ecosystem Dynamics, Section Population Biology, University of Amsterdam, The Netherlands.</footnote>
**Material and methods**

Specimens were slide-mounted using modified Hoyer's medium as described by Faraji and Bakker (2008) and examined using differential interference contrast microscopy. Illustrations were made using a drawing tube attached to an interference-phase contrast microscope (Nikon eclipse 80i). The tarsal ventro-terminal setae and other minute structures, e.g., spermatheca, famulus and solenidia, were re-examined and confirmed by means of a Zeiss Axio Imager 2 microscope. Images were taken using a Zeiss AxioCamHRc camera attached to the microscope, montaged with the Helicon Focus, and edited with Photoshop 2020. Idiosomal length was measured from the anterior rim to the posterior margin of the idiosoma and the width measured at the maximum width of the idiosoma between legs II and III. Setae were measured from the alveolus to the tip, and legs measured from the base of the trochanter to the tip of the claw. All measurements are given in micrometers (µm). Terminology of idiosomal chaetotaxy follows the hypothesis VI of Griffiths *et al.* (1990), that for palp and leg chaetotaxy follows Grandjean (1939) and Griffiths (1970). The holotype (circled) and three paratype females are deposited in the New Zealand Arthropod Collection, Landcare Research, Auckland, New Zealand (NZAC), and four paratype females are deposited in the Plant Health and Environment Laboratory, Auckland, New Zealand (PHEL). Acronyms of repositories follow Zhang (2018).

**Systematics**

**Oulenziella africana Fan and Faraji, sp. n.**

Zoobank: OBF45932-8131-4395-8A69-CD50F70EB6A9

(Figures 1–7, Table 1)

**Material examined**

Holotype female and 3 paratype females on the same slide, mango tree, 5 km west of Thika, Kenya, 23 Nov. 2016, by H. Wainwright; 4 paratype females one a slide, same collection data as holotype.

**Diagnosis**

ADULT FEMALE. Idiosomal setae *sce* about 2.8–3.4× as long as *vi* and 3.6–4.2× as long as *sci*; *sci-sci* 1.8–2.3× as wide as *sci-sce*; *h*₂ 1.3–1.9× as long as *c*ₚ and 4.7–6.9× as long as *h*₁; *c*ₚ 4.2–5.4× as long as *c*₁; *e*₁ 2.6–3.4× as long as *e*₂. Opisthonotal gland opening *gla* located much closer to *e*₂ than to *d*₂. Leg I with genual solenidia *σ''* 3.5–4.2× as long as *σ'*.

Male, larva and nymphs: Unknown.

**Description**

**Adult female**

(Figures 1–6)

Chelicerae robustly chelate (Figure 3C): fixed digit bearing 1 subterminal tooth, 3 large medial teeth and 1 small proximal tooth; movable digit bearing one small subterminal tooth, 3 large medial teeth, and 1 small proximal tooth; cheliceral setae *cha* small, conical, *chb* absent. Subcapitulum: setae *m* filiform (Figure 3D); palpal supracoxal setae *elcp* absent; dorsal and lateral palptibial setae filiform; dorsal palptarsal setae filiform, terminal palptarsal solenidion *ω* indistinct, present as rudimentary lines (Figure 3D).

Idiosoma (Figures 1 and 6A) oval, cuticle smooth. Prodorsal shield (Figures 1, 3A and 6D) nearly trapezoidal, finely stippled, posterior one forth areas with faint longitudinal to oblique wrinkles (Figure 6D), posterior margin medially convex and sublaterally concave. Supracoxal sclerite (Figures 3B and 6E) elongate; Grandjean's organ (Figures 1 and 3B) horn-shaped, smooth and short; supracoxal setae *scx* tapering from base to tip, medially barbed. Ocelli
<figure><img src="image_9.png" /><figcaption>**Figure 1** *Oulenziella africana* sp. n. (adult female). Dorsal view of idiosoma.</figcaption></figure>

(Figures 1, 3A and 6D) situated close to anterior corners of prodorsal shield; seta *vi* slightly longer than 4/5 of length of prodorsal shield; setae *ve* represented by alveoli, situated at lateral margins of prodorsal shield, posterior to ocelli; *sce* about 2.8–3.4× as long as *vi* and 3.6–4.2× as long as *sci*; *sci-sci* 1.8–2.3× as wide as *sci-sce*. Opisthonotal gland openings *gla* located closer to *e*₂ than to *d*₂, not distinctively coloured. Tiny opisthosomal tubercles visible on some specimens. Hysterosomal setae *h*₃ absent; *h*₂ longest, *sce*, *c*ₚ and *e*₁ obviously longer than others; *c*₁, *c*₂, *c*₃, *d*₁, *d*₂, *e*₂ and *h*₁ similar in length, *f*₂ positioned on ventral side of idiosoma and shorter than others; *e*₁ 2.6–3.4× as long as *e*₂, *h*₂ 1.3–1.9× as long as *c*ₚ and 4.7–6.9× as long as *h*₁; *c*ₚ 4.2–5.4× as long as *c*₁.

Coxal apodemes I (Figures 2 and 6B) joined at midline, forming a prosternal apodeme directed posteromedially; coxal plates I posteriorly extending beyond apex of prosternal apodeme, slightly concave posteromedially; coxal apodemes II (Figures 2 and 6B) directed posteromedially, coxal plates II large, extending far beyond apex of apodeme II, posterior margin concave posteromedially; sejugal apodeme faint, a simple ridge. Genital opening (Figures 2 and 6C) inverted V-shaped, situated medially between coxae III and IV, epigynal sclerite (Figure 2) situated immediately anterior to genital opening, umbrella-shaped in
<figure><img src="image_10.png" /><figcaption>**Figure 2** *Oulenziella africana* sp. n. (adult female). Ventral view of idiosoma.</figcaption></figure>

middle, extending laterally beyond inner tips of apodemes III; apodemes III directed medially; apodemes IV directed anteromedially and jointed with post-apodemes of coxae III. Ventral setae 1a inserted lateral to prosternal apodeme, 3a lateral to genital opening, g posterior to second pair of genital papillae, 4a posterior to lateral arms of genital opening. Anal opening far posterior to genital opening, slightly shorter than genital opening, surrounded by 3 pairs of pseudanal setae, ps₁ longest, about 4.6–5.5× as long as ps₂ and 4.8–6.1 ps₃. Copulatory opening close to posterior margin of idiosoma (Figures 3E and 6F); spermathecal duct (inseminatory canal) a thin cylindrical tube, slightly widening as it reaches spermathecal sac (seminal receptacle); sclerotised base of spermathecal sac bell-shaped, a pair of bell-shaped sclerites of oviducts situated at end of spermathecal sac, sclerites nearly as large as spermathecal base.

Legs. All setae on trochanters, femora, genua and tibiae smooth and attenuate.

Leg I (Figures 4A, 5A, C and 6G). Trochanter bearing 7–9 minute teeth on anteromedial
<figure><img src="image_11.png" /><figcaption>**Figure 3** *Oulenziella africana* sp. n. (adult female). A, prodorsal shield; B, supracoxal sclerite; C, chelicera; D, subcapitulum; E, copulatory opening and spermatheca.</figcaption></figure>

edge; femoral seta vF nearly extending to tip of genu; genual solenidia σ": σ'= 3.5–4.2, setae cG as long as mG; tibial solenidion φ obviously extending beyond tarsal claw tip, gT and hT subequal; tarsus about 3.7–4.1× as long as its basal width, ω₁ parallel sided and tapered at its apex, ε apically bifurcate and slightly shorter than ω₂, ω₃ slightly shorter than d, seta wa 1.9–2.4× as long as la and 2.1–2.6× as long as ra, d nearly extending to tarsal claw tip; e and f very small and e slightly longer than f; ventro-terminal spines s (4.5–5.0) conical; p and q subequal (3.0–3.5) and conical; u and v subequal (2.0) and apically truncate.

Leg II (Figures 4B, 5B, D and 6H). Trochanter bearing 7–9 minute teeth on anteromedial edge; femoral vF nearly extending to base of genual solenidion (σ), σ nearly extending to half-length of tibia, seta cG as long as or slightly shorter than mG; tibial solenidion φ obviously extending beyond tarsal claw tip, gT about as long as hT; tarsus about 4.1–4.6× as long as its basal width, ω tapering towards its apex, seta wa more than 2.3–3.2× as long as la and ra, d nearly extending to tarsal claw tip; e and f very small; ventro-terminal spines s (4.0–5.0) conical; p and q subequal (3.0–3.5) and conical; u and v subequal (2.0) and apically truncate.
<figure><img src="image_12.png" /><figcaption>**Figure 4** *Oulenziella africana* sp. n. (adult female). A, leg I; B, leg II; C, leg III; D, leg IV.</figcaption></figure>

Leg III (Figures 4C, 5E and 6I). Femur nude; genual solenidion σ very small, not extending to apical rim of genu; tibial solenidion φ slightly extending beyond tarsal claw tip, kT about as long as w on tarsus; tarsus about 6.1–6.9× as long as its basal width; r and w situated at same level, d nearly extending to tarsal claw tip; e and f very small but e slightly longer; ventro-terminal spines s (4.0–5.0) conical; p (3.5–4.5) longer than q (3.0–3.5), both conical; u (2.5–3.5) longer than v (2.0–3.0), both apically truncate.

Leg IV (Figures 4D and 5F). Trochanter, femur and genu nude; tibial solenidion φ about extending to tarsal claw tip, kT about as long as w on tarsus; tarsus more than 5.9–6.8× as long as its basal width; r situated proximal to w, d extending to base of tarsal claw; e and f absent; ventro-terminal spines s (4.0–5.0) conical; p (3.5–4.0) longer than q (2.5–3.0), both conical; u and v subequal (2.0–2.5) and both apically truncate.

Male, larva and nymphs

Unknown.
<figure><img src="image_13.png" /><figcaption>**Figure 6** *Oulenziella africana* sp. n. (adult female). A, anterior parts of dorsal idiosoma; B, leg coxae I–II; C, leg coxae III–IV and genital opening; D, prodorsal shield; E, supracoxal sclerite, supracoxal seta *scx* and duct of supracoxal gland; F, copulatory opening and spermatheca; G, dorsal view of apical tibia and proximal tarsus I; H, dorsal view of apical genu, tibia and proximal tarsus II; I, apical ventral view of tarsus III.</figcaption></figure>
<figure><img src="image_14.png" /><figcaption>**Figure 7** *Oulenziella africana* sp. n. A, newly formed egg; B, fully developed egg; C-E, food boli.</figcaption></figure>

$e_2$, $h_2$ 4.7–6.9× as long as $h_1$, and genu I with solenidion $\sigma''$ 3.2–4.0× as long as $\sigma'$ in adult females. The Kenyan specimen is consistent with all these key characters of females of *O. bakeri* described by Fan *et al.* (2015; 2020). Measurements on the adult female collected from Kenya are presented in Table 2.

**Redefinition of *Oulenziella* Fan and Zhang, 2015**

Due to the recent research (Barbosa & Moraes 2021; Fan *et al.* 2021; this work), a redefinition of the genus *Oulenziella* is presented below.

FEMALE. Ocelli strongly bulging, positioned on lateral margins of prodorsal shield in a position slightly posterior to *vi*; setae *sce* from nearly 3× to more than 8× as long as *sci*; supracoxal setae *scx* slender and scarcely barbed; hysterosomal setae $e_1$ 1.0–10.0× as long as $e_2$; $h_2$ from nearly 4.0× to more than 12× as long as $h_1$; each tarsus (excluding pretarsus) more than 4× as long as its basal width. Genu I with solenidion $\sigma''$ 3.0–9.5× as long as $\sigma'$; $\sigma$ on genu III small, usually not extending beyond anterior rim of segment; seta *d* on tarsi III and IV positioned at level of apical 1/8 to 1/6 of segment. Chaetotaxy and solenidiotaxy of legs I–IV: trochanter 1, 1, 1, 0; femora 1, 1, 0, 0; genua 2 + 2$\sigma$, 2 + 1$\sigma$, 1$\sigma$, 0; tibiae 2 + 1$\phi$, 2 + 1$\phi$, 1 + 1$\phi$, 1 + 1$\phi$; tarsi I with 4 long setae (*wa*, *ra*, *la* and *d*), 2 small apical setae (*f* and *e*) + 1 subterminal ventral spine (*s*) + 4 terminal ventral spines (*u*, *p*, *v* and *q*) + $\omega_1$ + $\omega_2$ + $\omega_3$ + 1$\epsilon$; tarsus II similar
<table><caption>**Table 2** Measurements of *O. bakeri* (adult female) collected from Kenya (n=1).</caption><thead><tr><th>Item</th><th>Measurements</th><th>Item</th><th>Measurements</th><th>Item</th><th>Measurements</th><th>Item</th><th>Measurements</th></tr></thead><tbody><tr><td>Idiosoma-L</td><td>314</td><td>Leg I</td><td>159</td><td>Leg II</td><td>159</td><td>Leg III</td><td>166</td></tr><tr><td>Idiosoma-W</td><td>173</td><td>vF I</td><td>46</td><td>vF II</td><td>45</td><td>σ III</td><td>3.5</td></tr><tr><td>Dorsal shield-L</td><td>66</td><td>cG I</td><td>30</td><td>cG II</td><td>18</td><td>kT III</td><td>28</td></tr><tr><td>Dorsal shield-W</td><td>67</td><td>mG I</td><td>26</td><td>mG II</td><td>26</td><td>φ III</td><td>108</td></tr><tr><td>vi</td><td>50</td><td>σ' I</td><td>15</td><td>σ II</td><td>15</td><td>r III</td><td>14</td></tr><tr><td>sci</td><td>41</td><td>σ" I</td><td>51</td><td>gT II</td><td>17</td><td>w III</td><td>21</td></tr><tr><td>sci-sci</td><td>35</td><td>gT I</td><td>18</td><td>hT II</td><td>20</td><td>d III</td><td>24</td></tr><tr><td>sci-sce</td><td>16</td><td>hT I</td><td>23</td><td>φ II</td><td>98</td><td>e III</td><td>4</td></tr><tr><td>sce</td><td>169</td><td>φ I</td><td>91</td><td>ω II</td><td>25</td><td>f III</td><td>3</td></tr><tr><td>scx</td><td>18</td><td>ω₁ I</td><td>18</td><td>la II</td><td>15</td><td>s III</td><td>4</td></tr><tr><td>c₁</td><td>32</td><td>ω₂ I</td><td>4</td><td>ra II</td><td>16</td><td>p III</td><td>3</td></tr><tr><td>c₁-c₁</td><td>52</td><td>ω₃ I</td><td>22</td><td>wa II</td><td>34</td><td>q III</td><td>2.5</td></tr><tr><td>c₁-d₁</td><td>41</td><td>ε I</td><td>3</td><td>d II</td><td>31</td><td>u & v III</td><td>1.5</td></tr><tr><td>c₂</td><td>41</td><td>la I</td><td>19</td><td>e II</td><td>4</td><td>Leg IV</td><td>192</td></tr><tr><td>cₚ</td><td>142</td><td>ra I</td><td>17</td><td>f II</td><td>3</td><td>kT IV</td><td>31</td></tr><tr><td>c₃</td><td>45</td><td>wa I</td><td>36</td><td>s II</td><td>4</td><td>φ IV</td><td>104</td></tr><tr><td>d₁</td><td>50</td><td>d I</td><td>22</td><td>p & q II</td><td>2.5</td><td>r IV</td><td>17</td></tr><tr><td>d₁-d₁</td><td>45</td><td>e I</td><td>4</td><td>u & v II</td><td>1.5</td><td>w IV</td><td>31</td></tr><tr><td>d₁-e₁</td><td>49</td><td>f I</td><td>3</td><td></td><td></td><td>d IV</td><td>35</td></tr><tr><td>d₂</td><td>46</td><td>s I</td><td>4</td><td></td><td></td><td>s IV</td><td>5</td></tr><tr><td>e₁</td><td>51</td><td>p & q I</td><td>2.5</td><td></td><td></td><td>p & q IV</td><td>3</td></tr><tr><td>e₁-e₁</td><td>39</td><td>u & v I</td><td>2</td><td></td><td></td><td>u & v IV</td><td>2</td></tr><tr><td>e₂</td><td>38</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>f₂</td><td>32</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>h₁</td><td>40</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>h₂</td><td>233</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>la</td><td>34</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>3a</td><td>31</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>3b</td><td>30</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>g</td><td>51</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>4a</td><td>30</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>ps₃</td><td>35</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>ps₂</td><td>34</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>ps₁</td><td>163</td><td></td><td></td><td></td><td></td><td></td><td></td></tr></tbody></table>

to tarsus I but without ω₂, ω₃ and ε; tarsus III with 3 long seta (w, r and d), 2 small apical setae (f and e) + 1 subterminal ventral spine (s) + 4 terminal ventral spines (u, p, v and q); tarsus IV with 3 long seta (w, r and d) + 1 subterminal ventral spine (s) + 2 terminal ventral spines (u, p, v and q).

MALE. Similar to female but genital opening with an aedeagus situated between coxae IV; sclerite anterior to genital opening contiguous with medial part of apodemes IV; ventral setae 3a absent; genital setae tiny, about as long as basal alveolus; tarsi I and II (excluding pretarsi) less than 3× as long as their basal width, their apicoventral portion modified into suckers; subterminal ventral spine (s) of tarsi I–II indiscernible; terminal ventral spines p and q of tarsi I and II blunt.

Oulenziinae currently comprises five genera. A key to genera of the subfamily (adult females) is presented as follows (Modified from Fan *et al.* 2015).

## Key to genera of the subfamily Oulenziinae (adult females)

<table><tr><td>1. Prodorsal shield with a pair of ocelli on its anterolateral margins</td><td>2</td></tr><tr><td>— Prodorsal shield without ocelli</td><td>4</td></tr></table>
2. Tibiae I and II each with one seta (gT); tibia IV without ventral seta; tarsus II without setae la or ra, tarsus III without seta w. *Oulenzia* Radford, 1950
— Tibiae I and II each with 2 setae (gT and hT); tibia IV with a ventral seta (kT); tarsus II with setae la and ra, tarsus III with seta w. 3

3. Genu I with σ'' more than 3× as long as σ'; ocellus with lateral margin strongly bulging. *Oulenziella* Fan and Zhang, 2015
— Genu I with σ": σ'=1 to 2; ocellus with lateral margin slightly recurved. *Procalvolia* Fain, 1971

4. Spermathecal duct long, forming 5-6 loops. *Psylloglyphus* Fain, 1966
— Spermathecal duct short, not forming loops. *Acalvolia* Fain, 1971

Discussion

There are relatively few morphological characters for identifying species of *Oulenziella* based on current knowledge. Useful characters include the comparative lengths of dorsal idiosomal setae (i.e., *e*₁: *e*₂, *h*₂: *h*₁) and the comparative length of solenidia σ'' and σ' of genu I. The male genitalia of *O. bakeri* and *O. longiseta* don't exhibit significant variability and is not considered a key character here. The structure of the female genitalia such as the base of spermatheca is potentially a useful character to separate *O. longiseta* from the other two species.

It is worth noting that the famulus (ε) on tarsus I of *O. africana* sp. n. is apically cleft (Figures 5A and 6G) though it is not an obvious feature. We re-examined ten females and ten males of *O. bakeri* described by Fan *et al.* (2020) and found that ε is cleft but can be inapparent when it is not in the correct visual position. We also examined six females and two males of *Acalvolia americana* Fan, George and Kumarasinghe (Fan *et al.* 2010), an undescribed female of *Oulenzia* from Malaysia (Fan *et al.* 2012), and four adult females of *Czenspinskia transversostriata* (Oudemans) collected in Turkey. Solenidion ε is generally conical with an obscure subapical barb on these specimens. Further observation on species of other genera needs to be done to determine whether this is a generic character for *Oulenziella*.

Barbosa and Moraes (2020) incorrectly listed *'Oulenzia arboricola'*: Baker & Warton, 1952, 342; Meyer & Rodriguez 1966: 24; Fan *et al.* 2012: 334.' under the species *Oulenzia arboricola* (Oudemans, 1928)' which should be removed from page 1042 of Barbosa and Moraes (2020). However, the authors did correctly treat *Oulenzia arboricola* sensu Baker & Warton, 1952 as *Oulenziella bakeri* (Hughes, 1962) on page 1043 (Barbosa & Moraes 2020). We confirm that, as in *O. bakeri*, the apicoventral portion of tarsi I and II of male *O. longiseta* is modified into a tarsal sucker and seta s is indiscernible based on images kindly provided by Barbosa and Moraes on 7 July 2021.

It is possible that mites of *Oulenziella* don't have a heteromorphic deutonymphal stage as discussed in Fan *et al.* (2020) who studied more than one thousand specimens of all life stages of *O. bakeri* in the collection of the Plant Health and Environment Laboratory, New Zealand.

Acknowledgements

We would like to thank Dr Henry Wainwright (Real IPM Company Ltd. Madaraka, Thika, Kenya) for providing us the mite material described in this study, Drs Gilberto J. de Moraes and Marina F. C. Barbosa (Universidade de São Paulo, Brazil) for re-examining type specimens of *Oulenziella longiseta*, and Dr W. Ho (Plant Health & Environment Laboratory, Ministry for Primary Industries) for identifying the fungus in the mite guts. The senior author is indebted to Drs Sherly George and Lalith Kumarasinghe and colleagues in Ministry for Primary Industries, New Zealand for encouraging the senior author's research.
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