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# Supplementary Material 1

## Tables S1–S7

**Authors:** Trofimets AV, Dufresnes C, Pawangkhanant P, Bragin AM, Gorin VA, Hasan M, Lalremsanga HT, Muin MA, Le DX, Nguyen TV, Suwannapoom C, Poyarkov NA (2024)

**Data type:** .pdf

**Explanation notes:** **Table S1.** Sequences and voucher specimens of the *Microhyla berdmorei* complex and outgroup taxa used in this study. Locality number corresponds to those shown on the map in Figure 1. An asterisk (*) indicates the specimens included in the ddRAD-seq analysis. — **Table S2.** Morphometric characters recorded from each specimen of *M. berdmorei* sensu stricto and *M. malcolmi* examined. (Continues on the next page). — **Table S3.** Primers used in this study. “F,” “L”-forward primer, “R,” “H”- reverse primer. — **Table S4.** Characteristics of analyzed single-marker DNA sequences and the proposed optimal evolutionary models for gene and codon partitions as estimated in PartitionFinder v1.0.1. Total length (in bp), number of conservative (Cons.), variable (Var.) and parsimony informative (Pars.-Inf.) sites, estimated Transition/ Transversion bias (R), and nucleotide frequencies (in %) are given (data presented only for the ingroup). The optimal partitioning scheme and model fit was estimated as suggested by the Akaike information criterion (AIC). — **Table S5.** Outgroup taxa used for divergence time estimation in *Microhyla berdmorei* complex. — **Table S6.** Calibration points for divergence time estimation. Node – tree node used for calibration, for node names see Figure S3; divergence time given in millions years (Ma. — **Table S7.** Summary of PC scores and statistics for the Principal Components Analysis (PCA) consisting of members of the *Microhyla berdmorei* species complex. Abbreviations are listed in the materials and methods.

**Copyright notice:** This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.

**Link:** https://doi.org/10.3897/vz.74.e127937.suppl1

# Supplementary Material 2

## Figures S1, S2

**Authors:** Trofimets AV, Dufresnes C, Pawangkhanant P, Bragin AM, Gorin VA, Hasan M, Lalremsanga HT, Muin MA, Le DX, Nguyen TV, Suwannapoom C, Poyarkov NA (2024)

**Data type:** .zip

**Explanation notes:** **Figure S1.** Phylogenetic relationships of the *Microhyla berdmorei* species group. B: ML-inference tree of *Microhyla* derived from the analysis of 3119 bp of concatenated alignment including 12S rRNA, tRNA<sup>Val</sup>, 16S rRNA, and COI mtDNA gene fragments. For voucher specimen information and GenBank accession numbers see Table S1. Numbers at tree nodes correspond to ML UFBS/BIPP support values, respectively. The color of the mitochondrial lineages in the tree corresponds to the color used in Figures 1–5. — **Figure S2.** Time tree of the *Microhyla berdmorei* complex. BEAST chronogram on the base of 3588 bp–long mtDNA + nuDNA dataset (12S rRNA, tRNA<sup>Val</sup>, 16S rRNA, and COI mtDNA genes + BDNF gene). Values at tree nodes correspond to median divergence age estimates; blue bars at tree nodes correspond to 95% HPD of divergence age estimates.

**Copyright notice:** This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.

**Link:** https://doi.org/10.3897/vz.74.e127937.suppl2