# Introduction of Typhlodromus (Anthoseius) recki in protected tomato crop with banker plants to control Aculops lycopersici

Benjamin Perrin, Benjamin Gard, michael goude, Benjamin Salvador, Amelie Bardel

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## To cite this version:

Benjamin Perrin, Benjamin Gard, michael goude, Benjamin Salvador, Amelie Bardel. Introduction of Typhlodromus (Anthoseius) recki in protected tomato crop with banker plants to control Aculops lycopersici. IOBC MEETING 2023 INTEGRATED CONTROL IN PROTECTED CROPS, TEMPERATE AND MEDITERRANEAN CLIMATE, Aug 2023, Brest, France. hal-04208097

HAL Id: hal-04208097

https://hal.inrae.fr/hal-04208097

Submitted on 15 Sep 2023

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Distributed under a Creative Commons Attribution 4.0 International License
# Introduction of *Typhlodromus (Anthoseius) recki* in protected tomato crop with banker plants to control *Aculops lycopersici*

¹B. Perrin, ²B. Gard, ¹M. Goude, ¹B. Salvador, ²A. Bardel

¹Agroecological Vegetable Systems Experimental Facility, INRAE, Mas Blanc, 66200 Alenya, France;

²CTIFL, Centre de Balandran, 751 Chemin de Balandran, 30127 Bellegarde, France

e-mail address: benjamin.perrin@inrae.fr

**Introduction:** The management of *A. lycopersici*, the Tomato Russet Mite (TRM), mainly rests on pesticides with partial efficacy and negative side effects. A European endemic Phytoseiidae: *Typhlodromus (Anthoseius) recki* has shown good predatory ability on TRM in lab experiments. Because no mass-rearing was available, we used banker plants to introduce this biocontrol agent. We selected *Mentha suaveolens* (mint) and *Phlomis fructicosa* (Jerusalem sage, here sage) on which *T. recki* naturally occurred. Two trials were conducted in greenhouse to evaluate whether *T. recki* achieved the control of TRM and whether mint and sage were suitable for installing the predatory mite on tomato.

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## Material and method

**Trial 1: Ability of *T. recki* to control TRM**
Under glasshouse, in soilless condition.

**6 Treatments, in separated compartments:**

* Untreated control

* Sulphur control: 2 spraying of Heliosoufre® at 7,5 L/ha

* 1 branch of mint + 30 *T. recki* / branch

* 1 branch of mint + 60 *T. recki* / branch

* 1 branch of sage + 60 *T. recki* / branch

* 1 branch of sage + 30 *T. recki* / branch

*T. recki* was introduced on each tomato plant and supplemented with *Typha* pollen (Nutrimite®).

Tomato plants were infested with TRM the same day.

⇒ **Measurement:** length of stem russetting on 20 tomato plants/treatment, at 7 dates

⇒ **Statistical analysis:** LM with Treatments as fixed effect followed by pairwise comparison, conducted for each date of observation.

**Trial 2: Suitability of mint and sage for installing *T. recki* on tomato:**
Under plastic tunnel, in soil

**2 Treatments, 3 blocks:**

* 2 pots of mint + 30 *T. recki* / pot

* 2 pots of sage + 30 *T. recki* / pot

*T. recki* was introduced on each tomato plant and supplemented with *Typha* pollen (Nutrimite®).

Tomato plants were infested with TRM the same day.

⇒ **Measurement:** n.o. *T. recki* on 6 leaflet/plant and 8 plants/block, at 6 dates

⇒ **Statistical analysis:** GLMM with banker plant species, time (DAI: days after introduction, factor), their interaction and distance from banker plant (numeric) as fixed effects, block as random effect + Pairwise comparison with FDR correction for each dates

## Results and discussion

The length of stem russetting was significantly reduced with the predatory mites compared to the untreated (from 89 to 99%) and to the sulphur controls (from 78 to 99%), regardless of the banker plant species and the predator density.

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*T. (A.) recki* was observed on tomato leaflets during all the experiment. We observed an increase in the population with significantly higher population at the end (42 DAI and 49 DAI) compared to the beginning of the experiment (Kruskal-Wallis, p<0.0001, data not shown).

Phytoseidae were observed

* at 0m from sage only until 34 DAI

* at 2,5m from both banker plant species at 50 DAI

* at 4,5m at from both banker plant species at 61 DAI

**Analysis of Deviance Table (Type II Wald chisquare tests)**

Response: n.o. *T.recki/leaflet*

<table><caption>Dispersal of *T. recki* from banker plants to tomato</caption><thead><tr><th></th><th>Chisq</th><th>Df</th><th>Pr(&gt;Chisq)</th></tr></thead><tbody><tr><td>Banker plant sp.</td><td>19.255</td><td>2</td><td>6.591e-05 ***</td></tr><tr><td>DAI</td><td>34.974</td><td>4</td><td>4.702e-07 ***</td></tr><tr><td>Distance from banker plant</td><td>16.226</td><td>1</td><td>5.623e-05 ***</td></tr><tr><td>Banker plant sp.:DAI</td><td>11.811</td><td>3</td><td>0.008058 **</td></tr></tbody></table>

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DAI: Days after introduction

Final populations were

* Significantly decreasing with distance from both banker plants

* Significantly higher with mint than sage

* very low for both species (<0,5/leaflet)

* Insufficient to control TRM (data not shown)

**Conclusion:** Our results confirm the interest of banker plants to introduce *T. (A.) recki* and its ability to control TRM. However, banker plant system did not control effectively TRM despite the good dispersion of the predatory, up to 4.5m from the banker plant. Further researches are needed to optimize feasibility, efficacy, and costs

Acknowledgments: We thank M. Douin for rearing and providing the predatory mites, R. Souriau for providing the pest inoculum and M.-S. Tixier for the original idea.

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