# Role of the metacommunity in the colonization of rabbit's intestinal tract to fight against antibiotic-resistant bacteria

Caroline Achard, Beatrice Gabinaud, Sylvie Combes, Véronique

Dupouy-Guiraute, Yuliaxis Ramayo Caldas, Catherine Denis, Maria Ballester
Davis Ballester, Nathalie Le Floc'H, Samuel Boucher, Stéphane Chatellier, et
al.

► To cite this version:

Caroline Achard, Beatrice Gabinaud, Sylvie Combes, Véronique Dupouy-Guiraute, Yuliaxis Ramayo Caldas, et al.. Role of the metacommunity in the colonization of rabbit's intestinal tract to fight against antibiotic-resistant bacteria. 16. International Symposium on Microbial Ecology - ISME16, Aug 2016, Montréal, Canada. hal-02799866

HAL Id: hal-02799866

https://hal.inrae.fr/hal-02799866v1

Submitted on 5 Jun 2020

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# Role of the metacommunity in the colonization of rabbit's intestinal tract to fight against antibiotic-resistant bacteria

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INSTITUT
CARNOT
ICSA

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ICSA
Institut Carnot
Santé Animale

Achard C.<fnref n="1" />, Gabinaud B.<fnref n="1" />, Combes S.<fnref n="1" />, Dupouy V.<fnref n="2" />, Ramayo-Caldas Y<fnref n="3" />, Denis C.<fnref n="3" />, Ballester M.<fnref n="3" />, Le Floc'h N.<fnref n="4" />, Boucher S.<fnref n="5" />, Chatellier S.<fnref n="6" />, Le Normand B.<fnref n="6" />, Bousquet-Melou A.<fnref n="2" />, Estellé J.<fnref n="3" />, Zemb O.<fnref n="1" />

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## Introduction and objective

* The (ab) use of antibiotics in farming may favour antibiotic resistant microbes, which may poses a risk to public health.

* Since these microbes may persist even if antibiotics become infrequent, an active strategy is required to limit their diffusion across the farm.

* Competitive exclusion with foreign microbes added to the nest may limit the transmission of persistant resistant microbes to the kits.

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## Methods and Experimental design

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### 1) Cultivable Enterobacteria

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Enterobacteria resistant to tetracycline (10 colonies per animal). The proportion of tetracycline resistant Enterobacteria in lacting does (orange bars) is stable and similar in each group. Adding chosen fecal pellets to the nest (blue bars) limits the transmission of resistant microbes.

### 2) Total antibiotic resistance genes

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Relative abundance of the microbial antibiotic resistance genes aphA3 (TOP) and tetO (BOTTOM) in the caecal content of the kits at weaning (normalized by the SSU). Adding Feces C decreased 6 out of the 20 antibiotic resistance genes that were quantified (vs 2 for Feces B). The does had similar levels of antibiotic resistance genes in each group (data not shown).

## Conclusions

* Changing the metacommunity of the nest changes the microbes colonizing the gut of the kits.

* The exclusion mecanism is not neutral and depends on the microbiota: Feces C seemed more efficient than Feces B.

* Some antibiotic resistance genes remained unaffected : the fecal community used for the competitive exclusion could be improved.

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<footnote n="1">¹INRA UMR 1388 GENPHYSE, Toulouse</footnote>

<footnote n="2">²INRA UMR 1331 TOXALIM, Toulouse</footnote>

<footnote n="3">³INRA UMR 1313 GABI, Jouy-en-josas</footnote>

<footnote n="4">⁴INRA UMR 1348 PEGASE, Rennes</footnote>

<footnote n="5">⁵Labovet, Reseau Cristal, Beaupréau et les herbiers</footnote>

<footnote n="6">⁶Clinique Veterinaire des Marches de Bretagne</footnote>

<footnote n="8">⁸Plateforme Genotoul, Toulouse</footnote>