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OPEN ACCESS

EDITED AND REVIEWED BY
Leo Marcelis,
Wageningen University and Research,
Netherlands

*CORRESPONDENCE

Zhengguo Li

<figure><img src="image_2.png" /></figure>

zhengguoli@cqu.edu.cn

RECEIVED 29 August 2023

ACCEPTED 08 September 2023

PUBLISHED 14 September 2023

CITATION

Liu Y, Hu G, Bouzayen M and Li Z (2023) Editorial: Advances in ripening regulation, quality formation, pre and post-harvest applications of horticultural products. *Front. Plant Sci.* 14:1285104.
doi: 10.3389/fpls.2023.1285104

COPYRIGHT

© 2023 Liu, Hu, Bouzayen and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

# Editorial: Advances in ripening regulation, quality formation, pre and post-harvest applications of horticultural products

Yudong Liu¹, Guojian Hu²,³, Mondher Bouzayen²,³ and Zhengguo Li³*

¹National Horticulture Germplasm Resources Center, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, China, ²Université de Toulouse, INRAe/INP Toulouse, Génomique et Biotechnologie des Fruits—UMR990, Castanet-Tolosan, France, ³Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, Chongqing, China

KEYWORDS

fruit, ripening, quality, phytohormone, transcription factor

Editorial on the Research Topic

Advances in ripening regulation, quality formation, pre and post-harvest applications of horticultural products

Horticultural crop products, such as fruits, provide important health-supporting
nutrients in human diet. The quality of fruits is closely related to consumer-liking,
influences fruit consumption and the development of fruit industry. Fruit ripening is a
complex process with dramatic changes in color, texture, flavor, aroma, and nutrient
metabolism that determines fruit quality formation. Fruit ripening is regulated by a
number of transcription factors (TFs) in conjunction with the plant hormones,
epigenetic modifications, as well as exogenous environment factors. Therefore, efforts to
decipher the mechanisms of ripening regulation and quality formation are crucial
contributing novel strategies to improve fruit quality. This Research Topic contains 4
original research articles that shed new light on the regulation mechanism of fruit ripening
and quality formation or provide effective strategies to improve fruit quality.

Yi et al. identified an ARF family transcription factor FveARF2, which is located in the nucleus, and plays as a negative regulator of strawberry fruit ripening and quality. By both silencing and overexpression of the FveARF2 gene in strawberry, the authors demonstrated that FveARF2 negatively regulates fruit earlier coloration, content of soluble solid, sugar, and anthocyanin through directly targeting genes associated with fruit quality formation, such as *FaSUT1*, *FaOMT*, and *FaCHS*. In addition, the authors also proposed that FveARF2 negatively regulate strawberry fruit quality by affecting potassium ion transport through directly binding to promoter of a potassium transporter gene *FveKT12*. The expression of FveARF2 is negatively correlated with content of potassium ion, and silencing FveARF2 increased potassium ion content in transgenic fruit. This study provides a new candidate gene resource for controlling strawberry fruit ripening and quality through molecular breeding.
Post-harvest ripening is a crucial course for banana fruit with significant conversion of starch. Xiao et al. screened the potential upstream regulators of MaGWD1, a key gene related to starch degradation of post-harvest banana fruit, and identified an ERF-AP2 family transcription factor, MaAP2a, could directly targets the promoter of MaGWD1. MaAP2a encompasses a transcriptional repressor domain (EAR, LxLxLx) with transcriptional inhibitory activity, and is located in the nucleus. Protein-DNA interaction assays such as electrophoretic mobility shift assay, Chromatin immunoprecipitation-qPCR analysis, and Dual-luciferase reporter assay demonstrated that MaAP2a directly binds to the promoters (GCC-box or AT-rich motif) of 15 starch degradation-related genes MaGWD1, MaPWD1, MaSEX4, MaLSF1, MaBAM1/2/3, MaAMY2B/2C/3A/3C, MaMEX1/2, and MapGlcT2-1/2-2, and repressed their activity. The authors confirmed a transcriptional repressor MaAP2a that participated in post-harvest banana ripening with a negative influence on starch degradation by directly targeting a series of starch degradation-related genes. This study shed light on the control of starch degradation of banana fruits during post-harvest ripening.

Phytohormones, such as ethylene (ET), gibberellin (GA), auxin and the interaction of them play important roles in fruit ripening and quality formation. Park and Malka performed transcriptome and metabolomic analysis of tomato fruit treated with ET, GA, and ET+GA to identify the potential molecular mechanism of ET and GA interplay in tomato fruit ripening. The authors found that ET and GA play opposite effects on auxin signaling and metabolite changes (such as sugar, amino acids, organic acids), and GA alone or combined with ET could inhibit ET signaling. This research indicated that auxin is involved in the interplay between ET and GA, enriched our understanding of the interaction of phytohormones during fruit ripening.

Li et al. evaluated the effects of alginate oligosaccharides (AOS) application on the antioxidant system, photosynthesis and fruit sugar accumulation in citrus through a two-year field experiment. The authors determined the optimal spray concentration and cycle of AOS on pre-harvest citrus fruit that could increase the content of soluble sugars and soluble solids. And the authors confirmed that the increase of sugar content in citrus fruits with AOS treatment may be related to the increased antioxidant enzyme activity and photosynthetic rate in leaves, as well as the transport of leaf assimilation product. Overall, the authors provide a potential application of AOS in the production of sugar enriched citrus fruits.

The articles in this Research Topic provide advanced information in fruit ripening regulation, quality formation, and pre-harvest applications. Further studies of endogenous and exogenous factors, such as phytohormones, TFs, epigenetic modifications, as well as environment factors that are involved in horticulture crops ripening and quality formation will be helpful to improve crop quality.

## Author contributions

YL: Writing - review & editing, Conceptualization, Project administration, Validation, Writing - original draft. GH: Writing - review & editing, Conceptualization, Project administration, Validation, Writing - original draft. MB: Conceptualization, Project administration, Validation, Writing - original draft, Writing - review & editing. ZL: Writing - review & editing.

## Acknowledgments

We thank all the authors that contributed to this Research Topic.

## Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision

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