# Diagnosing COVID-19-associated pulmonary aspergillosis

Paul E Verweij, J.-P. Gangneux, Matteo Bassetti, Roger J M Brüggemann, Oliver A Cornely, Philipp Koehler, Cornelia Lass-Flörl, Frank L van de Veerdonk, Arunaloke Chakrabarti, Martin Hoenigl

► To cite this version:

Paul E Verweij, J.-P. Gangneux, Matteo Bassetti, Roger J M Brüggemann, Oliver A Cornely, et al. Diagnosing COVID-19-associated pulmonary aspergillosis. The Lancet Microbe, 2020, 1 (2), pp.e53-e55. 10.1016/S2666-5247(20)30027-6. hal-03040673

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Submitted on 28 May 2021

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# Diagnosing COVID-19-associated pulmonary aspergillosis

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There is increasing concern that patients with coronavirus disease 2019 (COVID-19) might be at risk of developing invasive pulmonary aspergillosis co-infection.¹ In a cohort of 221 patients with COVID-19 in China, fungal infections were diagnosed in seven individuals, all of whom were admitted to the intensive care unit (ICU).² However, causative fungal pathogens were not identified.² Given that in China, galactomannan testing is rarely available,³ the real burden of invasive pulmonary aspergillosis in patients with COVID-19 requiring ICU admission is probably underestimated. Indeed, nine patients with COVID-19 and invasive pulmonary aspergillosis were recently described in France (33% of 27 admitted to the ICU with COVID-19),⁴ and five in Germany (26% of 19 admitted);⁵ rates similar to those observed in association with influenza.⁶ Although serum galactomannan is a sensitive diagnostic marker in patients with neutropenia in intensive care, galactomannan sensitivity was only 25% in patients who did not have neutropenia, but had proven invasive pulmonary aspergillosis.⁷ Although serum galactomannan was positive in 65% of patients with influenza-associated pulmonary aspergillosis,⁶ only three (21%) of 14 patients with COVID-19-associated pulmonary aspergillosis were serum galactomannan positive.⁴,⁵ Reasons for the lower sensitivity in patients with COVID-19 versus those with influenza are unknown, although treatment with chloroquine might have a negative effect on serum galactomannan performance, because the drug exhibits in-vitro activity against *Aspergillus fumigatus*.⁸ Exposure to antifungals is a well known factor that decreases the sensitivity of serum galactomannan testing.

Negative serum galactomannan might indicate that *Aspergillus* spp hyphae are unable to cause angioinvasive growth and release galactomannan into the blood. Most patients with COVID-19-associated pulmonary aspergillosis did not have European Organization for the Research and Treatment of Cancer/Mycoses Study Group Education and Research Consortium (EORTC/MSGERC) host factors, because only two (15%) of 13 had haematological malignancy as underlying disease.³ Absence of host factors was also apparent in influenza-associated disease, in which 57% of patients could not be classified according to the EORTC/MSGERC consensus definition or the AspICU algorithm for patients

in ICUs. A retrospective multicentre cohort study showed that influenza infection was an independent risk factor for invasive pulmonary aspergillosis.⁶ In addition to local erosion of the epithelial barrier of the respiratory tract, influenza virus can exhibit a direct immunomodulatory effect through suppression of the NADPH oxidase complex.⁹ Suppression of the NADPH oxidase complex might cause a temporary disease status resembling chronic granulomatous disease, which itself is associated with invasive pulmonary aspergillosis development.⁹ Severe COVID-19 is associated with immune dysregulation, affecting both T-helper cell 2 (Th2) and Th1 responses,¹⁰ although this has not been extensively studied and a direct immunomodulatory effect on the known antifungal host defence has not been demonstrated. During the first severe acute respiratory syndrome coronavirus (SARS-CoV) outbreak in 2003, only four cases of proven invasive pulmonary aspergillosis were reported among 8422 probable SARS cases.³ Because all four invasive pulmonary aspergillosis cases were associated with concomitant corticosteroid therapy, coronavirus infection itself might not increase the risk for invasive pulmonary aspergillosis, but other risk factors might have.

Bronchoalveolar lavage galactomannan testing is important to diagnose invasive pulmonary aspergillosis in the ICU and high galactomannan levels (galactomannan index >2.5) were observed in patients with presumed COVID-19-associated pulmonary aspergillosis.⁵ However, only a restricted role for bronchoscopy has been recommended in COVID-19, because it is an aerosol-generating procedure that poses risks to patients and personnel.¹¹ Collection of upper respiratory samples is the preferred method for diagnosis, and tracheal aspirates and non-bronchoscopic alveolar lavage in intubated patients.¹¹ Although *Aspergillus* spp can be detected in sputum and tracheal aspirates in patients with COVID-19-associated pulmonary aspergillosis, its presence might reflect oral pharyngeal colonisation because *Aspergillus* spp is considered a core component of the basal oral mycobiome. Furthermore, galactomannan testing is not validated for upper respiratory tract specimens. Bronchoscopy is recommended in COVID-19 only when the intervention is considered lifesaving, which

Published Online

May 8, 2020

https://doi.org/10.1016/
S2666-5247(20)30027-6

This online publication has
been corrected. The corrected
version first appeared at
thelancet.com/microbe on
May 20, 2020
**Panel: Crucial next research questions for COVID-19 associated invasive pulmonary aspergillosis**

Diagnosis of CAPA

* What is the positive predictive value of culture isolation of Aspergillus species in samples from the upper respiratory tract (infections vs colonisation)?

* In light of low sensitivity of serum galactomannan, are there alternative blood tests for CAPA? What is the performance of Aspergillus PCR, β-D-glucan, and the Aspergillus—specific lateral flow device and lateral flow assay?

* Do radiological signs differ in people with CAPA from what's seen in COVID-19 without CAPA

Prophylaxis and treatment of CAPA

* Is the incidence of CAPA in intensive care unit patients high enough to justify antifungal prophylaxis trials?

* What is the clinical relevance of CAPA? Is there a survival benefit with antifungal treatment?

* What is the CAPA-associated mortality? Autopsy study vs post-mortem lung biopsy?

* What is the optimal treatment of CAPA? Considerations of efficacy, dosing, adverse events, and drug-drug interactions

Immunology or host factors

* Underlying host factors (neutropenia, lymphopenia, monocytopenia, polymorphisms; eg, PTX3, dectin-1, and NADPH-oxidase) or a role for concomitant medication, such as (hydroxy)chloroquine being either harmful (causing defective autophagy) or having direct antifungal or protective effect like in chronic granulomatous disease?

* Role of the kallikrein-kinin system in antifungal host defense

* Is there an underlying antifungal defect caused by COVID-19 or associated with CAPA, such as defective reactive oxygen species production, defective T-helper cell 1 responses, defective LC3-associated phagocytosis, or defective neutrophil extracellular traps activation or release, such as in chronic granulomatous disease?

CAPA=COVID-19-associated pulmonary aspergillosis. COVID-19-coronavirus disease 2019.

includes secondary infectious causes.¹¹ Radiological and clinical signs of invasive pulmonary aspergillosis in non-neutropenic patients are mostly unspecific and bronchoscopy is thus indicated in critically ill patients with COVID-19 who are suspected of secondary infection, including fungal diagnostic work-up. Even if evidence for Aspergillus spp is recovered, uncertainty remains about whether patients truly develop invasive disease and require antifungal therapy. Indeed, eight of the nine patients with COVID-19-associated pulmonary

aspergillosis from France were not treated with antifungal drugs, and the three deaths were considered not to be related to aspergillosis, but clinically attributed to bacterial septic shock.³ Autopsies were not performed to confirm the clinical diagnosis.

It is therefore crucial to gain insight into the inter-
action between Aspergillus spp and the SARS-CoV-2-
infected lung (panel). Only histopathology can prove
invasive pulmonary aspergillosis through autopsy of
deceased patients with COVID-19-associated pulmonary
aspergillosis. If autopsy is precluded because of the risk
of aerosol formation, post-mortem lung biopsy might
be considered as an alternative to obtaining tissue. Until
histopathological evidence of COVID-19-associated
pulmonary aspergillosis is obtained, we believe that
patients with COVID-19 who are critically ill with evidence
for Aspergillus spp in bronchoalveolar lavage or serum
should receive antifungal therapy according to national
and international guidelines.

PEV reports grants from Gilead Sciences, Merck Sharp & Dohme (MSD), Pfizer, ThermoFisher, and F2G; and non-financial support from OLM Diagnostics and IMMY, outside the submitted work. J-PG reports grants and personal fees from Pfizer and MSD outside the submitted work. MB reports grants and personal fees from Pfizer, MSD, Gilead, Angelini, Basilea, Astellas, Cidara, and Shionogi; personal fees from Biomerieux, Vifor, Paratek, Nabriva, Achaogen, Menarini, Bayer, Tetraphase, Venatorx, and Vifor; and grants from Melinta, outside the submitted work. RJMB served as consultant for Astellas Pharma, F2G, Amplyx, Gilead Sciences, MSD, and Pfizer; and has received unrestricted and research grants from Astellas Pharma, Gilead Sciences, MSD, and Pfizer; all contracts were through Radboudumc, and all payments were invoiced by Radboudumc. OAC is supported by the German Federal Ministry of Research and Education; is funded by the Deutsche Forschungsgemeinschaft (German Research Foundation) under Germany's Excellence Strategy (CECAD, EXC 2030 - 390661388); has received research grants from Actelion, Amplyx, Astellas, Basilea, Cidara, Da Volterra, F2G, Gilead, Janssen Pharmaceuticals, Medicines Company, MedPace, Melinta Therapeutics, MSD, Pfizer, and Scynexis; is a consultant to Actelion, Alleca Therapeutics, Amplyx, Astellas, Basilea, Biosys UK, Cidara, Da Volterra, Entasis, F2G, Gilead, Matinas, MedPace, Menarini Ricerche, Roche Diagnostics, MSD, Nabriva Therapeutics, Octapharma, Paratek Pharmaceuticals, Pfizer, PSI, Rempex, Scynexis, Seres Therapeutics, Tetraphase, and Vical; and received lecture honoraria from Astellas, Basilea, Gilead, Grupo Biotoscana, MSD, and Pfizer. PK has received non-financial scientific grants from Miltenyi Biotec, Bergisch Gladbach, Germany, and the Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases, University of Cologne, Cologne, Germany; and is an advisor to, or received lecture honoraria from Akademie für Infektionsmedizin, Astellas Pharma, Gilead Sciences, GPR Academy Ruesselsheim, MSD, Noxxon Pharma, and University Hospital, Ludwig-Maximilians-Universität Munich, outside the submitted work. CL-F reports grants, personal fees, and payment of accommodation or travel from Gilead Sciences; grants and payment of accommodation or travel from Astellas Pharma; personal fees from MSD; personal fees and payment of accommodation or travel from Basilea; and payment of accommodation or travel from Angelini Pharma, outside the submitted work. FLvdV has served as an invited speaker for Gilead Sciences. AC declares no competing interests. MH reports grants from Pfizer and Gilead outside the submitted work.

Copyright © 2020 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 license.

*Paul E Verweij, Jean-Pierre Gangneux, Matteo Bassetti, Roger J M Brüggemann, Oliver A Cornely, Philipp Koehler, Cornelia Lass-Flörl, Frank L van de Veerdonk,*
Arunaloke Chakrabarti, Martin Hoenig, on behalf of the
European Confederation of Medical Mycology,
the International Society for Human and Animal Mycology,
the European Society for Clinical Microbiology and
Infectious Diseases Fungal Infection Study Group, and the
ESCMID Study Group for Infections in Critically Ill Patients
paul.verweij@radboudumc.nl

Departments of Medical Microbiology (PEV), Department of Pharmacy (RJMB), and Department of Infectious Diseases (FLvdV), Radboud University Medical Centre, Nijmegen, Netherlands; Center of Expertise in Mycology Radboudumc/CWZ, Nijmegen, Netherlands (PEV, RJMB, FLvdV); University of Rennes, Centre Hospitalier Universitaire (CHU) de Rennes, Inserm, EHESP, Institut de Recherche en Santé Environnement et Travail - UMR_S 1085, Rennes, France (J-PG); Clinica Malattie Infettive, Ospedale Policlinico San Martino, Istituto di Ricovero e Cura a Carattere, Genoa, Italy (MB); Department of Health Sciences, University of Genoa, Genoa, Italy (MB); Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (OAC, PK), Department I of Internal Medicine, University Hospital Cologne (OAC, PK), and Excellence Center for Medical Mycology, University Hospital Cologne (OAC, PK), Faculty of Medicine, University of Cologne, Cologne, Germany; German Centre for Infection Research, Partner Site Bonn-Cologne, Cologne, Germany (OAC); Clinical Trials Centre Cologne, Cologne, Germany (OAC); Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria (CL-F); Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India (AC); Division of Infectious Diseases and Global Health, University of California San Diego, La Jolla, CA, USA (MH); and section of Infectious Diseases and Tropical Medicine, Medical University of Graz, Graz, Austria (MH)

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