# Diamond micro-cantilevers as transducers for olfactory receptors - based biosensors

Raafa Manai, Dounia Kamouni-Belghiti, Marie-Annick Persuy, M. Habchi, Lionel Rousseau, M. Possas Abreu, P. Bergonzo, Edith Pajot, Guenhaël Sanz, E. Scorsone

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## ► To cite this version:

Raafa Manai, Dounia Kamouni-Belghiti, Marie-Annick Persuy, M. Habchi, Lionel Rousseau, et al. Diamond micro-cantilevers as transducers for olfactory receptors - based biosensors. International Conference on Nanomedicine and Nanobiotechnology 2016, Sep 2016, Paris, France., 2016. hal-02740147

HAL Id: hal-02740147

https://hal.inrae.fr/hal-02740147

Submitted on 2 Jun 2020

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Distributed under a Creative Commons Attribution 4.0 International License
ICONAN 2016
International Conference on NANOMEDICINE
And NANOBIOTECHNOLOGY

BOOK OF ABSTRACTS

University Pierre and Marie Curie, Paris

Sept 28-30, 2016

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PremC

Conferences, Events & Workshops

premc.org/iconan2016
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materials at peak performance

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* Batch to batch consistency

* Precision engineered nanoparticles

* Supplied with full characterization data

Visit
Particle
Works
on Patio 25!

www.particle-works.com

nano lane
EYES ENTERING THE NANOWORLD

N-Lab
Station

New label-free surface analysis equipment

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* Live nanoscale imaging

* Direct quantitative analysis

* Multiplex surface interaction study

* Thermal and fluidic control

* Mode: Air/Liquid/Gas

* Microfluidic compatibility
# Committee

## Chairwoman

Ane-Marie Caminade, University of Toulouse

## Scientific Committee

Albana Halili, Epoka University

William Waissmann, Oswaldo Cruz Foundation / São Paulo State University

Christophe Ramseyer, Université de Bourgogne Franche-Comté

Iwona Cicha, University Hospital Erlangen, Section of Experimental Oncology and Nanomedicine (SEON)

Niko Hildebrandt, Université Paris-Sud

Jeffrey Keelan, University of Western Australia

Mariano Venanzi, University of Rome 'Tor Vergata'

Maria Blanco-Prieto, University of Navarra

Bjørn Torger Stokke, The Norwegian University of Science and Technology, NTNU

Fabio Pastorino, IRCCS Istituto G. Gaslini

Suna Timur, Ege University

Wafa Al-Jamal, UEA

Serge Ravaine, Bordeaux University

Andre G Skirtach, Ghent University

Vadim Kessler, SLU

Ana Belén Gonzalez-Guerrero, Catalan Institute on Nanoscience and Nanotechnology

Kenneth E. Gonsalves, IIT Mandi Himachal Pradesh 175001

Luis Liz-Marzán, CIC biomaGUNE

David Fairen-Jimenez, University of Cambridge

Rongjun Chen, Imperial College London

Florence Gazeau, Paris Diderot University

Sijumon Kunjachan, Harvard Medical School

Claire Billotey, Université de Lyon, UJM, EMR 3738

Stéphane Roux, Institut UTINAM, Université de Franche-Comté

Nadine Millot, Université de Bourgogne Franche-Comté

Christophe Moreau, Institut de Biologie Structurale, Grenoble

Casuso Ignacio, INSERM U1006 - BioAFM Lab

James N Sturgis, Aix-Marseille Université

Eric Finot, University of Burgundy

Eric Lesniewska, University of Bourgogne / ICB UMR CNRS 6303

Edith Pajot-Augy, INRA

Abdelhamid Errachid, The Institute of Analytical Sciences

Ibtisam E Tothill, Cranfield University

Gaëlle Offranc Piret, U1205 INSERM-UGA-CHU

Rogério Gaspar, University of Lisbon

Thierry Pécot, INRIA Rennes - Bretagne Atlantique

Emilie Roger, Angers University

Nicolas Bertrand, Université Laval

Fabien Picaud, Université de Bourgogne Franche-Comté

Sebastien Balme, Université de Montpellier

Etienne Dague, CNRS

Min-Hui Li, Chimie PariesTech - ENSCP

Ciro Chiappini, Imperial College London

Fernando Torres Andón, Istituto Clinico Humanitas

Ana Luísa Cardoso, FCA - State University of Campinas

Nejat Düzgüneş, University of the Pacific School of Dentistry

Dongyang Li, University of Alberta
Seda Kizilel, Koc University

Zofia Lipkowska, Institute of Organic Chemistry, Polish Academy of Sciences

**Plenary Speakers**

Anne-Marie Caminade, University of Toulouse

Ick Chan Kwon, Korea Institute of Science and Technology

Dennis E. Discher, University of Pennsylvania

Jackie Ying, Institute of Bioengineering and Nanotechnology,

Rabah Boukherroub, Lille 1 University

Fabian Kießling, ExMI – Experimental Molecular Imaging

Claire Wilhelm, Paris Diderot University

Kenneth A. Dawson, University College Dublin

Christian Serre, ENS-ESPCI-PSL-CNRS

Philippe Barthélémy, Bordeaux Segalen University

Elias Fattal, University of Paris-Sud

Kostas Kostarelos, University of Manchester

**Organizing Committee**

PremC
# Table of Contents

<table><tr><td>Theragnostic Nanoparticles for Drug Delivery Systems<br>Prof. Ick Chan Kwon</td><td>1</td></tr><tr><td>Magnetic nanoparticles in cell therapies: from combined approaches in cancer treatment to magnetic tissue engineering.<br>Prof. Claire Wilhelm</td><td>2</td></tr><tr><td>Nano-antibiotics: A rational design of functional nanoparticles to combat bacterial infection<br>Dr. Rabah Boukherroub</td><td>3</td></tr><tr><td>Considerations on the design of nanoparticles for diagnostic, theranostic and therapeutic purposes<br>Prof. Fabian Kiessling</td><td>4</td></tr><tr><td>Chitosan-Collagen hybrid 3D-scaffolds as potential biomaterials for tissue engineering<br>Ms. Hilary Urena, Dr. Marianelly Esquivel-Alfaro, Dr. Sergio Madrigal-Carballo</td><td>5</td></tr><tr><td>Corona interactome: a key for deciphering protein adsorption kinetics on silica nanocarriers<br>Mr. Cédric Pisani, Mr. Jean-charles Gaillard, Mr. Michaël Odorico, Dr. Jeff Nyalosaso, Dr. Clarence Charnay, Dr. Yannick Guari, Prof. Joël Chopineau, Prof. Jean-marie Devoisselle, Dr. Jean Armengaud, Dr. Odette Prat</td><td>7</td></tr><tr><td>Design of mesoporous Fe3O4/MS nanoparticles as drug delivery platform of prednisolone<br>Mrs. Ivalina Trendafilova, Prof. Margarita Popova, Prof. Agnes Szegedi, Prof. Judith Mihály, Prof. Denitsa Momekova, Prof. Georgi Momekov, Dr. Irina Nikolova, Mr. Lyubomir Marinov</td><td>8</td></tr><tr><td>Cytotoxic Evaluation of Irinotecan-loaded PLGA-PEG-retinoic acid Nanomicelles on HT-29 Cancer Cell Line<br>Dr. Parnian Maghzi, Dr. Jaber Emami, Dr. Farshid Hasanzadeh, Dr. Hojjat Sadeghi</td><td>10</td></tr><tr><td>PREPARATION, CHARACTERIZATION AND RADIOLABELED OF VENLAFAXINE-PLGA NANOPARTICLES WITH 99mTC FOR BRAIN DELIVERY<br>Ms. María Dolores Cayero, Ms. ROCÍO IGLESIAS JEREZ, Mr. Roque Salazar Cabrera, Ms. Isabel Borrego Dorado, Dr. Lucía Martín-Banderas</td><td>12</td></tr><tr><td>Capsaicin-Coated silver nanoparticles inhibit amyloid fibril formation of serum albumin<br>Mr. Bibin G Anand, Dr. Karunakar Kar, Mrs. Kriti Dubey, Mr. Dolat Singh shekhawat</td><td>13</td></tr><tr><td>Diamond micro-cantilevers as transducers for Olfactory Receptors - based biosensors<br>Dr. Raafa Manai, Dr. Dounia Kamouni-Belghiti, Dr. Marie-Annick Persuy, Ms. Massiel Habchi, Dr. Lionel Rousseau, Ms. Maira Possas Abreu, Dr. Philippe Bergonzo, Dr. Edith Pajot-Augy, Dr. Guenhaël Sanz, Dr. Emmanuel Scorsone</td><td>14</td></tr><tr><td>Diphtheria toxin-derived protein for targeted delivery of nanoparticles<br>Mr. Mahesh Agarwal, Dr. Amaresh Sahoo, Dr. Biplab Bose</td><td>16</td></tr></table>
<table>
  <tbody>
    <tr>
      <td>Formulation and In-Vitro Evaluation of Enteric Coated Capsules Filled with Recombinant Human Keratinocyte Growth Factor Loaded Chitosan Nanoparticles<br>Dr. Palanirajan Vijayaraj Kumar, Dr. Yeong Siew Wei, Mr. Mhd Luay Takahje, Dr. Lee Ming Tatt, Prof. Abu Bakar Bin Abdul Majeed, Dr. Vasudevan Mani</td>
      <td>17</td>
    </tr>
    <tr>
      <td>Molecular Imprints for the Detection of Specific Glycoproteins Implicated in Cancer<br>Ms. Philippa Mitchell, Dr. Lewis Hart, Prof. Paula Mendes</td>
      <td>19</td>
    </tr>
    <tr>
      <td>Optimizing liposomes delivering arsenic trioxide to cervical cancer cells in vitro<br>Ms. Anam Akhtar, Dr. Xuesong Wen, Dr. Scarlet Wang, Dr. Lucy Ghali, Dr. Celia Bell</td>
      <td>21</td>
    </tr>
    <tr>
      <td>Study Effects of Synchronize Utilize of Gamma-ray and Silver Nano Particles Treatment on Human Prostate Cancer Cells<br>Dr. alireza shams</td>
      <td>22</td>
    </tr>
    <tr>
      <td>RADIOLABELED NANOPARTICLES STRATEGIES FOR BREAST CANCER THERAGNOSIS<br>Dr. Lucía Martín-Banderas, Ms. ROCÍO IGLESIAS JEREZ, Mrs. Mº Dolores Cayero-Otero, Dr. Alejandro Perera, Mr. Roque Salazar Cabrera, Ms. Isabel Borrego Dorado</td>
      <td>23</td>
    </tr>
    <tr>
      <td>Mesoporous silica nanoparticles surrounded by a lipid bilayer: Absence of in vitro hemonanotoxicity<br>Dr. Christina Grigorakaki, Dr. Vincent Schlesser, Dr. Céline Hoffmann, Dr. Jean-Sébastien Thomann, Ms. Gaëlle Corne, Dr. César Pascual Garcia, Dr. Sivashankar Krishnamoorthy, Prof. Rolf Bjerkvig, Dr. Valérie Palissot</td>
      <td>25</td>
    </tr>
    <tr>
      <td>Novel Synthetic Method and Characterization of Magnetic Resonance/Near-infrared dual-modal imaging agents by In Vivo Molecular Imaging<br>Mr. Hyunseung Lee, Ms. Hyun Min Kim, Dr. Jee-Hyun Cho, Mr. Jongeun Kang, Dr. Hye Sun Park, Dr. Kwan Soo Hong</td>
      <td>27</td>
    </tr>
    <tr>
      <td>3D Printing Revolution for Nanotechnology and Nanomedicine<br>Dr. Alessandro Paolini, Ms. Antonella Celluzzi, Dr. Antonella Baldassarre, Dr. Simona Sennato, Dr. Francesco Mura, Dr. Federico Bordi, Dr. Alberto Eugenio Tozzi, Dr. Andrea Masotti</td>
      <td>28</td>
    </tr>
    <tr>
      <td>Synthesis of High Sensitive Magnetic Nanoparticles and Application for Inflammation Detection<br>Mr. Jongeun Kang, Mr. Hyunseung Lee, Dr. Kwan Soo Hong</td>
      <td>29</td>
    </tr>
    <tr>
      <td>Use of cell membrane permeating peptides for delivery of plasmid DNA into dendritic cells<br>Ms. Dominika Hobernik, Dr. A. James Mason, Prof. Stephan Grabbe, Dr. Matthias Bros</td>
      <td>30</td>
    </tr>
    <tr>
      <td>Efficient gene silencing with siRNA formulated in DOP-DETA-based lipid nanoparticles<br>Dr. Tomohiro Asai, Ms. Mariko Sako, Ms. Jiao Li, Ms. Ayaka Okamoto, Dr. Takehisa Dewa, Prof. Naoto Oku</td>
      <td>31</td>
    </tr>
    <tr>
      <td>Glimepiride solid lipid nanoparticles: Formulation, Evaluation and In-Vivo study.<br>Dr. omneya khowessah</td>
      <td>32</td>
    </tr>
    <tr>
      <td>A novel immunosensor based on polypyrrole electroless deposition on silicon nitride substrates: Interleukine-10 detection<br>Ms. Faiza Nessark, Dr. Abdoullatif Baraket, Dr. Nadia Zine, Prof. Ahmed Zouaoui, Prof. Belkacem Nessark, Prof. Joan Bausells, Prof. abdelhamid Errachid</td>
      <td>34</td>
    </tr>
    <tr>
      <td>Biophysical Characterization, Nanoscale Composition and Cell Uptake Studies of pH-Sensitive Drug Delivery Systems<br>Dr. Jana B. Nieder, Ms. Rasa Ozolina, Ms. Ana M. Carvalho, Ms. Vânia Vilas-Boas, Prof. M.E.C.D. Real Oliveira, Dr. Marlene Lúcio</td>
      <td>35</td>
    </tr>
  </tbody>
</table>
Effect of Nanosilver with salicylic acid & nano salicylic acid pre-treatment on Antioxidant responses in Dracocephalum moldavica L. 36

Prof. Hossein Abbaspour, Dr. zahra haghighipak, Dr. Naser Karimi

Formulation of Levofloxacin Loaded Niosomal suspensions as an Ocular Delivery System: In-Vitro Evaluation and Microbiological Study. 37

Dr. salwa hussien

Sickle cell hemoglobin detection in drying drops: from protein/nanoparticle interaction to low resource diagnostic tools 39

Dr. Stephanie Devineau, Dr. Manos Anyfantakis, Mr. Laurent Marichal, Dr. Laurent Kiger, Prof. Damien Baigl

Molecular mechanism and increased antileishmanial activity of carbon nanotube based betulin formulation 41

Dr. Prakash Saudagar

At focus compressed 7 and 70 femtosecond pulses for deep tissue multiphoton microscopy 42

Dr. Christian Maibohm, Dr. Francisco Silva, Dr. Edite Figueiras, Dr. Paulo T. Guerreiro, Dr. Marina Brito, Dr. Rosa Romero, Dr. Helder Crespo, Dr. Jana B. Nieder

In vivo anti-inflammatory activity by trans-resveratrol loaded-solid lipid nanoparticle for skin disorders. 43

Ms. Roberta Rigon, Ms. Maíra Gonçalez, Ms. Camila Rodero, Dr. Marlus Chorilli

Porous metal-organic-framework MIL-100(Fe) as a nanoscale platform for sustainable release of tetracycline 44

Mr. Seyed Dariush Taherzade, Dr. Aliakbar Tarlani, Dr. Janet Soleimannejad

Characterisation of polyurethane nanocomposite hydrogel systems for wound dressing application. 46

Ms. Marta Miotke, Dr. Justyna Strankowska, Prof. Marek Józefowicz, Dr. Michał Strankowski, Prof. Jerzy Kwela

Curcumin-loaded cationic solid lipid nanoparticles as a potential platform for the treatment of melanoma48

Ms. Maíra Gonçalez, Ms. Roberta Rigon, Dr. Marlus Chorilli

Peptide functionalised nanoparticles for the selective induction of apoptosis in target cells 50

Prof. Mervin Meyer, Dr. Nicole Sibuyi, Dr. Ntevheleni Thovhogi, Prof. Martin Onani, Dr. Amanda Skepu, Dr. Abram Madiehe

Thermosensitive nanogels with multiple anti-tumour associated effects 51

Dr. Malou Henriksen-Lacey, Mr. Malte Strozyk, Dr. Susana Carregal, Prof. Mathias Brust, Prof. Luis Liz-Marzán

Promising New NanoTheranostic Quantum dots Based on Ag2S-PEG-FA 53

Prof. havva yagci acar

Study of RNA interference mediated by lipid-coated calcium phosphate nanoparticle transfection in high-grade gliomas. 55

Dr. Laura Pandolfi, Dr. Miriam Colombo, Mrs. Benedetta Santini, Mrs. Lucia Salvioni, Dr. Svetlana Avvakumova, Prof. Silvia Nicolis, Prof. Davide Prosperi

Nanometronomics: doxorubicin-loaded H-ferritin allows for tailored treatment of breast cancer based on lower doses and higher safety 56

Dr. Serena Mazzucchelli, Ms. Michela Bellini, Dr. Marta Truffi, Dr. Luisa Fiandra, Ms. Maria Antonietta Rizzuto, Dr. Luca Sorrentino, Dr. Fabio Corsi, Prof. Davide Prosperi
<table>
  <tbody>
    <tr>
      <td>Turn-on and Color-changeable Fluorogenic Sensor Created by the 10BASEd-T<br><u>Prof. Masumi Taki</u></td>
      <td>57</td>
    </tr>
    <tr>
      <td>One-step DNA Detection through Dual-Color Confocal Analysis of DNA-Assembled Scattering Nanoparticles: Case of a Fragment of Sesame<br><u>Dr. Stephanie VIAL, Mr. Youri BERRAHAL, Dr. Marta PRADO, Dr. Jérome WENGER</u></td>
      <td>59</td>
    </tr>
    <tr>
      <td>Up-converting and down-converting nanoparticle-based aptasensor model for multiplex detection of foodborne pathogens<br><u>Dr. Hasan Kurt, Dr. Meral Yüce, Mr. Babar Hussain, Prof. Hikmet Budak</u></td>
      <td>61</td>
    </tr>
    <tr>
      <td>Analysis of the controlled drug release (CDR) from biopolymer nanoparticles during the initial burst using a novel modeling method<br><u>Ms. Cristiana de Azevedo, Dr. Moritz Von Stosch, Prof. Rui Oliveira</u></td>
      <td>62</td>
    </tr>
    <tr>
      <td>Detection of cytokines using biosensor based on functionalized nanocarriers growth on gold electrode<br><u>Prof. Abdelhamid Errachid, Ms. Sahar Chaibi, Dr. toufik hadjersi, Mr. Abaidia Seddik El Hak, Dr. Abdoulatif Baraket, Prof. Joan Bausells, Dr. yaakoubi nourdin, Dr. Nadia Zine</u></td>
      <td>63</td>
    </tr>
    <tr>
      <td>Bio-compatibilised carbon nanotubes display significant anti-tumoral effects in solid melanomas<br><u>Dr. Mónica L. Fanarraga, Mrs. Eloisa González-lavado, Dr. Lorena García-hevia, Ms. Carmen Pesquera, Mr. Fernando González, Dr. Jesús González, Dr. Juan C Villegas, Dr. Rafael Valiente</u></td>
      <td>64</td>
    </tr>
    <tr>
      <td>Targeting of TRAIL conjugated maghemite nanoparticles for biomedical applications<br><u>Mrs. Hanene BELKAHLA, Dr. Myriana Hemadi, Dr. Guillaume Herlem, Dr. Olivier Micheau, Prof. Souad Ammar, Prof. Tijani Gharbi</u></td>
      <td>66</td>
    </tr>
    <tr>
      <td>New promising Glucose-Metal Nanoparticles for potential applications in Radiotherapy<br><u>Dr. Francesco Porcaro, Prof. Chiara Battocchio, Prof. Antonio Antoccia, Dr. Ilaria Fratoddi, Dr. Iole Venditti, Dr. Anna Fracassi, Dr. Igor Luisetto, Dr. Andrea Ugolini, Prof. Maria Vittoria Russo, Prof. Giovanni Polzonetti</u></td>
      <td>68</td>
    </tr>
    <tr>
      <td>Microfluidic synthesis and biological evaluation of photothermal biodegradable copper sulphide nanoparticles<br><u>Ms. Isabel Ortiz de Solorzano, Mr. Martín Prieto, Dr. Gracia Mendoza, Dr. Teresa Alejo, Dr. Silvia Irusta, Dr. Víctor Sebastián, Dr. Manuel Arruebo</u></td>
      <td>70</td>
    </tr>
    <tr>
      <td>Development of Innovative Multistage Nanovectors for Cancer Immunotherapy<br><u>Ms. Flavia Fontana, Dr. Mohammad-Ali Shahbazi, Dr. Dongfei Liu, Dr. Hongbo Zhang, Mr. Ermei Mäkilä, Prof. Jarno Salonen, Prof. Jouni Hirvonen, Dr. Helder A. Santos</u></td>
      <td>71</td>
    </tr>
    <tr>
      <td>Thrombolytic therapy based on P-selectin targeted polymer nanoparticles<br><u>Ms. Maya Juenet, Ms. Rachida Aid-launais, Dr. Véronique Ollivier, Ms. Alice Berger, Mr. BO LI, Dr. Didier Letourneur, Dr. Cédric Chauvierre</u></td>
      <td>73</td>
    </tr>
    <tr>
      <td>Uptake and intracellular localization of engineered gold nanoparticles in A549 cells.<br><u>Ms. Abiola Dosumu, Ms. Shani Osborne, Prof. Zoe Pikramenou, Dr. Nik Hodges</u></td>
      <td>75</td>
    </tr>
    <tr>
      <td>Development of polymer microcapsules functionalized with fucoidan to target P-selectin under arterial flow conditions<br><u>Mr. BO LI, Ms. Maya Juenet, Dr. Véronique Ollivier, Ms. Rachida Aid-launais, Dr. Didier Letourneur, Dr. Cédric Chauvierre</u></td>
      <td>77</td>
    </tr>
  </tbody>
</table>
<table>
  <tbody>
    <tr>
      <td>Ligand Tethered Gold Nanoparticles against Untamed and Drug Resistant Leishmania Donovani<br>Prof. Arup Mukherjee, Mr. Asim Halder, Dr. Suvadra Das</td>
      <td>79</td>
    </tr>
    <tr>
      <td>Harnessing human blood to examine bio-nano interactions at the cellular level<br>Mr. Joshua J Glass, Ms. Liyu Chen, Dr. Michael Whittaker, Ms. Sarah Mann, Prof. Edmund Crampin, Dr. John Quinn, Ms. Ewa Czuba, Dr. Kristofer Thurecht, Dr. Georgina Such, Prof. Thomas Davis, Dr. Angus Johnston, Dr. Robert De Rose, Prof. Stephen Kent</td>
      <td>81</td>
    </tr>
    <tr>
      <td>Injectable thermoresponsive magnetic hydrogel composite incorporating iron oxide and hydroxyapatite nanoparticles for bone tissue engineering<br>Dr. Padmalosini Muthukumaran, Prof. Seeram Ramakrishna, Prof. Balázs Gulyás, Prof. Raju V. Ramanujan, Prof. Dinesh Kumar Srinivasan</td>
      <td>82</td>
    </tr>
    <tr>
      <td>Biogenic Gold Nanoparticles for Complete Recovery of Dermal Burn Wounds<br>Dr. Suvadra Das, Mr. Asim Halder, Dr. Partha Roy, Ms. Anwesha Banerjee, Mr. Durbadal Ojha, Mr. Saptarshi Mandal, Dr. Debprasad Chattopadhyay, Prof. Arup Mukherjee</td>
      <td>84</td>
    </tr>
    <tr>
      <td>WORKSHOP: Different approaches for the formation of synthetic hydrogels based on hybrid physically-chemically cross-linked networks<br>Dr. Maxime Grillaud, Mr. Maarten Bakker, Dr. Patricia Dankers</td>
      <td>86</td>
    </tr>
    <tr>
      <td>The Drive to Master the Foundation Principles of Nanoscale interactions with living Organisms<br>Prof. Kenneth Dawson</td>
      <td>88</td>
    </tr>
    <tr>
      <td>Biocompatible Metal Organic Frameworks in Nanomedicine<br>Dr. Christian Serre</td>
      <td>89</td>
    </tr>
    <tr>
      <td>Dendrimers as tools towards nanomedicine<br>Dr. Anne-Marie Caminade</td>
      <td>90</td>
    </tr>
    <tr>
      <td>From Nano Shape &amp; Self Recognition to Flexibility in Cancer Treatment and Differentiation<br>Prof. Dennis Discher</td>
      <td>92</td>
    </tr>
    <tr>
      <td>Magnetic force-based skeletal muscle tissue engineering for in vitro drug testing<br>Dr. Akira Ito, Mr. Kazushi Ikeda, Mr. Ryusuke Imada, Dr. Masanori Sato, Dr. Yoshinori Kawabe, Prof. Masamichi Kamihira</td>
      <td>93</td>
    </tr>
    <tr>
      <td>Characterization of Noble Metal Nanoparticles functionalized by molecule-capping method with mixed organic ligands carried out by SR-XPS and SERS<br>Ms. Laura Carlini, Prof. Chiara Battocchio, Prof. Paolo Postorino, Ms. Claudia Fasolato, Dr. Ilaria Fratoddi, Dr. Iole Venditti, Ms. Giovanna Testa, Dr. Fabio Sciubba</td>
      <td>94</td>
    </tr>
    <tr>
      <td>Incorporation of paclitaxel into hollow-p4VP nanoparticles to improve breast cancer chemotherapy<br>Ms. Maria del Carmen Leiva Arrabal, Ms. Julia Jiménez-López, Dr. Rafael Contreras-Caceres, Ms. Laura Cabeza, Dr. Gloria Perazzoli, Dr. Raúl Ortiz, Prof. Consolación Melguizo, Prof. Juan Manuel López-Romero, Prof. José Prados</td>
      <td>96</td>
    </tr>
    <tr>
      <td>Tannin-chitosan composite nanoparticles as potential nanomedicine to prevent urinary tract infections<br>Dr. Sergio Madrigal-Carballo, Ms. Emilia Alfaro-Viquez, Dr. Christian Krueger, Prof. Jess Reed</td>
      <td>98</td>
    </tr>
    <tr>
      <td>Lung cancer: a new approach to paclitaxel treatment using PLGA nanocarriers<br>Ms. Julia Jiménez-López, Ms. Maria del Carmen Leiva Arrabal, Dr. Mazen El-Hammadi, Ms. Laura Cabeza, Dr. Gloria Perazzoli, Dr. Lucía Martín-Banderas, Dr. Raúl Ortiz, Prof. José Prados, Prof. Consolación Melguizo</td>
      <td>100</td>
    </tr>
  </tbody>
</table>
<table>
  <tbody>
    <tr>
      <td>Nanofibers Preparation by Free-Liquid Surface Electrospinning for Cartilage Tissue Engineering<br>Ms. Parinita Agrawal, Prof. Krishna Pramanik</td>
      <td>102</td>
    </tr>
    <tr>
      <td>Evaluation of chloroaluminium phthalocyanine-loaded magnetic nanoemulsion as drug delivery device to treat glioblastoma using hyperthermia and photodynamic therapy<br>Dr. Leonardo Barcelos de Paula, Prof. Fernando Lucas Primo, Prof. Marcelo Rodrigues Pinto, Prof. Paulo Cesar Morais, Prof. Antonio Claudio Tedesco</td>
      <td>103</td>
    </tr>
    <tr>
      <td>Synthesis, research and functionalization of hybrid contrast agents based on gadolinium doped mag-netite<br>Mrs. Iana Tcareva, Prof. Alexander Majouga, Prof. Alexander Savchenko, Mr. Maksim Abakumov, Mr. Igor Shchetinin</td>
      <td>104</td>
    </tr>
    <tr>
      <td>In-depth investigation on DNA-AgNCs designs for adenosine detection<br>Ms. Shi Ting Lee, Dr. Siu Yee New</td>
      <td>105</td>
    </tr>
    <tr>
      <td>Noncovalent Assembly of Carbon Nanotubes: Toward the Construction of Nanotube-Based Breast Cancer Therapy Nanovectors<br>Dr. Ayhan Unlu, Mr. Mehdi Partovi Meran, Ms. Bircan Dinc, Mr. Yasin Celikok, Prof. Isil Albeniz, Prof. Seniha Guner</td>
      <td>106</td>
    </tr>
    <tr>
      <td>Molecular dynamics simulation of interaction of lysine dendrimer and Semax peptide<br>Mrs. Elena Popova, Prof. Igor Neelov, Mr. Victor Kuznetsov, Mr. Sergey Petunov, Mr. Angrey Radilov</td>
      <td>107</td>
    </tr>
    <tr>
      <td>Salinomycin nanoparticles induce selective toxicity toward tumor cells rather than stroma in orthotopic model of pancreatic cancer<br>Dr. zahra Daman, Dr. Yuhua Wang, Prof. Leaf Huang</td>
      <td>108</td>
    </tr>
    <tr>
      <td>Antibacterial effects of gold-chitosan nanocomposites on human macrophages infected by intracellular pathogenic bacteria<br>Dr. Gracia Mendoza, Ms. Anna Regiel-futyra, Dr. Vanesa Andreu, Dr. Víctor Sebastián, Dr. Agnieszka Kyzioł, Prof. Grażyna Stochel, Dr. Manuel Arruebo</td>
      <td>110</td>
    </tr>
    <tr>
      <td>Multifunctional polymer-modified liposomes that capture and neutralize toxic protein, histones<br>Mr. Hiroki Tsuchida, Dr. Hiroyuki Koide, Mr. Masahiko Nakamoto, Ms. Anna Okishima, Ms. Saki Ariizumi, Ms. Chiaki Kiyokawa, Dr. Tomohiro Asai, Prof. Yu Hoshino, Prof. Naoto Oku</td>
      <td>111</td>
    </tr>
    <tr>
      <td>Hypoxia-directed and activated theranostic agent: Imaging and treatment of solid tumor<br>Ms. Hyun Min Kim, Prof. Eun-joong Kim, Mr. Hyunseung Lee, Prof. Jong Seung Kim, Dr. Kwan Soo Hong</td>
      <td>112</td>
    </tr>
    <tr>
      <td>Chemically cross-linked silk fibroin hydrogel with enhanced elastic properties, biodegradability, and bio-compatibility<br>Mr. minhee kim, Mr. seung hyun lee, Prof. Won Ho Park</td>
      <td>113</td>
    </tr>
    <tr>
      <td>Non-invasive In Vivo Imaging and Tracking of Dendritic Cells Migration using MR/NIR Dual Modal Con-trast Agent<br>Dr. Jee-Hyun Cho, Ms. Hyun Min Kim, Dr. Hye Sun Park, Dr. Kwan Soo Hong</td>
      <td>115</td>
    </tr>
    <tr>
      <td>Matryoshka-type enteric microparticles for the treatment of tuberculosis<br>Dr. Vanesa Andreu, Ms. Ane Larrea, Dr. Salvador Alfaro, Ms. Begoña Gracia, Dr. Gracia Mendoza, Dr. Víctor Sebastián, Dr. José Antonio Ainsa, Dr. Manuel Arruebo</td>
      <td>116</td>
    </tr>
  </tbody>
</table>
<table>
  <tbody>
    <tr>
      <td>Regulation of angiogenesis through the efficient delivery of microRNAs into endothelial cells using polyamine-coated carbon nanotubes<br>Dr. Andrea Masotti, Dr. Mark Miller, Ms. Antonella Celluzzi, Dr. Lorrain Rose, Dr. Federico Micciulla, Dr. Patrick Hadoke, Dr. Stefano Bellucci, Dr. Andrea Caporali</td>
      <td>117</td>
    </tr>
    <tr>
      <td>How to enhance the functionality of microencapsulated cells by using graphene oxide nanoparticles<br>Dr. Laura Saenz del Burgo, Dr. Jesús Ciriza, Dr. Argia Acarregui, Mr. Haritz Gurruchaga, Prof. Rosa María Hernández, Dr. Gorka Orive, Prof. Jose Luis Pedraz</td>
      <td>118</td>
    </tr>
    <tr>
      <td>Cellular internalization mechanisms of polyamine-coated carbon nanotubes<br>Ms. Antonella Celluzzi, Dr. Alessandro Paolini, Dr. Andrea Masotti</td>
      <td>120</td>
    </tr>
    <tr>
      <td>Synthesis of polymer coated Co0.5Zn0.5Fe2O4 nanoparticles and their cytotoxicity on human carcinoma cells<br>Dr. Zulqurnain Ali, Dr. Rashda Abbasi, Dr. Muhammad Atif, Ms. Javeria Arshad, Mr. Abdul Jabbar Khan, Dr. Nafees Ahmad</td>
      <td>121</td>
    </tr>
    <tr>
      <td>Ratiometric real-time measurement of protein kinase activity with fluorophore labeled polyion complexes<br>Mr. Takanobu Nobori, Prof. Akihiro Kishimura, Prof. Takeshi Mori, Prof. Yoshiki Katayama</td>
      <td>123</td>
    </tr>
    <tr>
      <td>Synthetic hydrogels based on hybrid physically-chemically cross-linked networks<br>Dr. Maxime Grillaud, Mr. Maarten Bakker, Dr. Patricia Dankers</td>
      <td>125</td>
    </tr>
    <tr>
      <td>A Novel niosome gene delivery approach for central nervous system disorders<br>Mr. Mohamed Mashal, Dr. Noha Attia, Prof. Gustavo Puras, Prof. Jon Zarate, Dr. Cristina Soto Sanchez, Prof. Eduardo Fernandez, Prof. Jose Luis Pedraz</td>
      <td>127</td>
    </tr>
    <tr>
      <td>Proteins adsorption upon nanoparticles: from the physicochemical basis to the functional impacts<br>Mr. Laurent Marichal, Dr. Jean-philippe Renault, Dr. Jean-christophe Aude, Dr. Yves Boulard, Dr. Serge Pin, Dr. Jean Labarre</td>
      <td>128</td>
    </tr>
    <tr>
      <td>Flexible cortical Multi-electrode array implant for neural recording in minipig<br>Mr. Jean-Marie Mayaudon, Dr. Lionel Rousseau, Dr. Gaëlle Offranc Piret, Dr. Blaise Yvert</td>
      <td>130</td>
    </tr>
    <tr>
      <td>Exudate Triggered Metal Corrosion for Self-Powered Wound Healing Application<br>Mr. Sun Woong Han, Mr. Keun Ho Lee, Mr. Tae Hoon Ki, Prof. Hong Koo Baik</td>
      <td>131</td>
    </tr>
    <tr>
      <td>Collagen glycation versus chronologically-aged fibroblasts in keratinocyte differentiation of reconstructed human skin<br>Ms. Roberta Rigon, Mr. Christian Hausmann, Mr. Christopher Wolff, Dr. Julia Tigges, Prof. Ellen Fritsche, Dr. Marus Chorilli, Dr. Christian Zoschke, Prof. Monika Schäfer-korting</td>
      <td>132</td>
    </tr>
    <tr>
      <td>Nanoformulation of imipramine loaded resealed erythrocytes as potent anti-leishmanial, which targets unique 'prokaryotic TopA' homolog in Leishmania<br>Ms. Sumedha Mukherjee, Ms. Devyani Shukla, Dr. Somdeb Bosedasgupta</td>
      <td>134</td>
    </tr>
    <tr>
      <td>Platinum nanoparticles as multifunctional active nanocarriers integrating the function of high-performance antioxidant drugs<br>Ms. Deborah Pedone, Dr. Elisa De Luca, Dr. Mauro Moglianetti, Dr. Roberto Marotta, Mr. Tiziano Catelani, Dr. Barbara Sartori, Dr. Heinz Amenitsch, Prof. Saverio Francesco Retta, Dr. Pier Paolo Pompa</td>
      <td>136</td>
    </tr>
  </tbody>
</table>
<table>
  <tbody>
    <tr>
      <td><b>Transport of Liposome Encapsulated Drugs in Voxelized Computational Model of Brain Tumors</b><br>Mr. Ajay Bhandari, Dr. Ankit Bansal, Dr. Anup Singh, Dr. Niraj Sinha</td>
      <td>138</td>
    </tr>
    <tr>
      <td><b>Sensitization of sarcoma tumors with short chain sphingolipid liposomes</b><br>Dr. Sara Zalba Oteiza, Mr. Joost A. P. Rens, Dr. Jeroen Rovers, Prof. Marcel Verheij, Dr. Timo L. M. Ten Hagen</td>
      <td>140</td>
    </tr>
    <tr>
      <td><b>Immune checkpoint blockade in melanoma by new targeted Doxorubicin immunoliposomes</b><br>Mrs. María Merino Díaz, Mrs. Ana Margarita Contreras Sandoval, Dr. Noelia Casares, Dr. Iñaki Troconiz, Dr. Pedro Berraondo, Dr. Timo L. M. Ten Hagen, Dr. Sara Zalba Oteiza, Dr. Maria J Garrido</td>
      <td>141</td>
    </tr>
    <tr>
      <td><b>Titanate nanotubes as new preclinical theranostic platform against prostate cancer: vectorization and immobilization of docetaxel or of gold nanoparticles</b><br>Mr. Alexis Loiseau, Dr. Julien Boudon, Dr. Céline Mirjolet, Prof. Gilles Créhange, Prof. Stéphane Roux, Prof. Nadine Millot</td>
      <td>142</td>
    </tr>
    <tr>
      <td><b>The endothelial glycocalyx controls interactions of nanoparticles with the endothelium and their translocation across the blood-tissue border</b><br>Dr. Bernd Uhl, Dr. Stephanie Hirn, Mr. Roland Immler, Mrs. Karina Mildner, Dr. Leonhard Möckl, Prof. Markus Sperandio, Prof. Christoph Bräuchle, Dr. Christoph Reichel, Dr. Dagmar Zeuschner, Prof. Fritz Krombach</td>
      <td>143</td>
    </tr>
    <tr>
      <td><b>Magnetic hyperthermic response of nanocomposites based on PEG2000-gallol-coated iron oxide nanoparticles dispersed in poly(N-isopropylacrylamide-co-acrylamide) hydrogels</b><br>Mr. Kevin C. Behan, Ms. Sarah M. Martyn, Dr. Aylvin A. Dias, Prof. Andreas Heise, Dr. Dermot F. Brougham</td>
      <td>144</td>
    </tr>
    <tr>
      <td><b>Increasing the stability and antimicrobial activity of antimicrobial peptides after association to Lipid nanocapsules</b><br>Ms. Nada Matougui, Dr. Anne Claire Groo, Dr. Anita Umerska, Dr. Helena Bysell, Prof. Patrick Saulnier</td>
      <td>146</td>
    </tr>
    <tr>
      <td><b>Biological Recognition of Biomolecular Corona</b><br>Ms. Sandra Lara, Ms. Fatima Alnasser, Dr. Ester Polo, Dr. David Garry, Ms. Maria-cristina Lo-giudice, Dr. Delyan Hristov, Dr. Yan Yan, Prof. Kenneth A. Dawson</td>
      <td>148</td>
    </tr>
    <tr>
      <td><b>Synthesis-Dependent Surface Defects and Morphology of Hematite Nanoparticles and Their Effect on Cytotoxicity in Vitro</b><br>Mr. Dean Cardillo, Dr. Moeava Tehei, Dr. Md Shahriar Hossain, Mr. Md Monirul Islam, Ms. Kathrin Bogusz, Dr. Dongqi Shi, Dr. David Mitchell, Prof. Michael Lerch, Prof. Anatoly Rosenfeld, Dr. Stéphanie Corde, Dr. Konstantin Konstantinov</td>
      <td>149</td>
    </tr>
    <tr>
      <td><b>Fate of Various Dendrimers with Different Sizes and Surfaces after Subcutaneous and Intradermal Administration</b><br>Prof. Chie Kojima</td>
      <td>151</td>
    </tr>
    <tr>
      <td><b>Specific nanoparticle targeting of the EGF-receptor using single-domain antibodies.</b><br>Dr. Kristof Zarschler, Dr. Louise Rocks, Dr. Eugene Mahon, Dr. Kanlaya Prapainop, Dr. Holger Stephan, Prof. Kenneth A. Dawson</td>
      <td>153</td>
    </tr>
    <tr>
      <td><b>Differential Modulation of Biological Properties In Vitro by a Range of cRGDY Peptides on Clinically Translated Dual-Modality Silica Nanoparticles</b><br>Dr. Miriam Benezra, Dr. Pauliah Mohan, Prof. Ulrich Weisner, Prof. Michelle Bradbury</td>
      <td>154</td>
    </tr>
    <tr>
      <td><b>Traceable Iron Oxide Based Nanoparticles for Antigen/Adjuvant in vivo Delivery to Lymph Nodes</b><br>Ms. Ane Ruiz de Angulo, Dr. Aintzane Zabaleta, Ms. Zuriñe Baz, Dr. Jordi Llop, Prof. Juan Carlos Mareque Rivas</td>
      <td>156</td>
    </tr>
  </tbody>
</table>
<table>
  <tbody>
    <tr>
      <td>Magnetic-nanoparticles as a theranostic tool for liver metastases in a murine model<br>Mr. Borja Herrero De La Parte, Dr. Eneko Garayo Urabayen, Dr. Oihane Kistiñe Arriortua Llarena, Dr. Jose Javier Echevarria-uraga, Prof. Jose Angel Garcia Martinez, Prof. Ignacio García-Alonso Montoya, Prof. Fernando Plazaola Muguruza, Ms. Irati Rodrigo Arrizabalaga</td>
      <td>158</td>
    </tr>
    <tr>
      <td>Hybrids of biomolecules and carbon nanotubes: nanodevices for biosensing<br>Prof. KAZUO UMEMURA, Mr. Shusuke Oura, Mr. Yu Ishizaka</td>
      <td>160</td>
    </tr>
    <tr>
      <td>Biomimetic compartmentalization approach in designing nanoreactor with organelle-like function<br>Dr. Vimalkumar Balasubramanian, Ms. Alexandra Correia, Dr. Hongbo Zhang, Ms. Flavia Fontana, Mr. Ermei Mäkilä, Prof. Jarno Salonen, Prof. Jouni Hirvonen, Dr. Helder A. Santos</td>
      <td>161</td>
    </tr>
    <tr>
      <td>NBelyax® Nanoparticles for disinfection and sterilization of living and nonliving surfaces tested in vitro and in situ<br>Dr. Leon Albarran, Mrs. Gabriela León, Mr. Sergio León, Dr. Paola Arteaga</td>
      <td>163</td>
    </tr>
    <tr>
      <td>Cloning, expression and purification of Pseudomonas aeruginosa azurin, a small redox protein and its application in molecular electronics<br>Ms. Neeti Kalyani, Prof. Prashant Mishra</td>
      <td>164</td>
    </tr>
    <tr>
      <td>A modified Hodgkin-Huxley model for nanoelectronics<br>Prof. Peter Burke</td>
      <td>166</td>
    </tr>
    <tr>
      <td>WORKSHOP: SEEC Microscopy, a live and label-free analysis technique in the fields of Materials and Life Sciences<br>Mr. Nicolas Medard, Mr. Imed Ayadi</td>
      <td>168</td>
    </tr>
    <tr>
      <td>Functionalization of Emissive Conjugated Polymer Nanoparticles by Coprecipitation: Consequences for Particle Photophysics and Colloidal Properties<br>Prof. Gareth Redmond</td>
      <td>169</td>
    </tr>
    <tr>
      <td>Innovative SPIONs for multimodal imaging: MRI/PET and MRI/optical imaging<br>Dr. Julien Boudon, Dr. Guillaume Thomas, Dr. Lionel Maurizi, Prof. Nadine Millot</td>
      <td>171</td>
    </tr>
    <tr>
      <td>Elaboration of a new in vivo imaging system based on multimodal upconversion nanoparticles<br>Mr. Julien Santelli, Dr. Lechevallier Séverine, Dr. Robert Mauricot, Prof. Daniel Cussac, Prof. Marc Verelst</td>
      <td>173</td>
    </tr>
    <tr>
      <td>The Curious Case of 1D and 2D Carbon Nanostructure Pharmacology & Toxicology<br>Prof. Kostas Kostarelos</td>
      <td>174</td>
    </tr>
    <tr>
      <td>Nucleic acid chemistry for nanomedicine<br>Prof. Philippe Barthelemy</td>
      <td>175</td>
    </tr>
    <tr>
      <td>Nanotechnologies for targeted delivery of nucleic acid<br>Prof. Elias Fattal</td>
      <td>177</td>
    </tr>
    <tr>
      <td>Nanostructured Biomaterials for Medical and Biological Applications<br>Jackie Ying</td>
      <td>178</td>
    </tr>
    <tr>
      <td>Investigating the Eremostachys laciniata (EL) and Curcumin Longa (CL) encapsulated by Solid Lipid Nanoparticles (SLN) in order to treat the inflammatory diseases<br>Mr. akbar vaseghi, Ms. Haleh Seyedabasi, Ms. Mina Ghanbari, Dr. Alireza Panahi, Ms. Elaheh Alizadeh, Mr. Bager Karimi, Mr. Reza Ashrafi Parchin</td>
      <td>179</td>
    </tr>
  </tbody>
</table>
<table>
  <tbody>
    <tr>
      <td>Effects of paclitaxel delivery by carbon nanotubes on prostate cancer cells and monocytes<br>Mr. Edson Comparetti, Dr. Valber Pedrosa, Dr. Ramon Kaneno</td>
      <td>180</td>
    </tr>
    <tr>
      <td>Nanoscale Engineering of Hybrid Magnetite-Carbon Nanofibre Materials for MRI Contrast Agents<br>Ms. Olga Metelkina, Dr. Graham Rance, Dr. Galina Pavlovskaya, Prof. Alexander Savchenko, Prof. Andrei Khlobystov, Prof. Alexander Majouga, Dr. Anastasia Garanina</td>
      <td>182</td>
    </tr>
    <tr>
      <td>Cytotoxicity of ICD-85 NPs on Human Cervical Carcinoma HeLa Cells through Caspase-8 Mediated Pathway<br>Prof. Abbas Zare Mirakabadi</td>
      <td>184</td>
    </tr>
    <tr>
      <td>Will a Carbon Nanosheet serve as a replacement to membrane components in a Nanodisc?<br>Dr. Suresh Vepuri, Prof. Mahmoud Soliman, Prof. Thirumala Govender</td>
      <td>185</td>
    </tr>
    <tr>
      <td>Accumulation of Doxorubicin Conjugates with Dendritic Polymers and Vector Protein in Normal and Tumor Cells in vitro<br>Prof. Irina Zamulaeva, Ms. Olga Matchuk, Dr. Nikita Yabarov, Ms. Elena Nikolskaja</td>
      <td>186</td>
    </tr>
    <tr>
      <td>Cytotoxic Effects of Ionizing Radiation and Doxorubicin Conjugates with Dendritic Polymer and Vector Protein on Breast Cancer Cells in vitro<br>Ms. Olga Matchuk, Ms. Kristina Churyukina, Dr. Nikita Yabarov, Ms. Elena Nikolskaja, Prof. Irina Zamulaeva</td>
      <td>187</td>
    </tr>
    <tr>
      <td>Mutagenicity testing in non-transformed and transformed human breast cell lines after exposure to silver nanoparticles in combination with aluminum chloride, butylparaben, or di-n-butylphthalate<br>Dr. Maciej Stepnik, Ms. Katarzyna Domeradzka-Gajda, Dr. Joanna Roszak, Dr. Anna Kozajda, Ms. Anna Smok-Pieniązek, Prof. Jarosław Grobelny, Dr. Emilia Tomaszewska, Prof. Grzegorz Celichowski, Dr. Małgorzata Cieślak, Dr. Dorota Puchowicz</td>
      <td>188</td>
    </tr>
    <tr>
      <td>Electrospun PCL nanofibers loaded with carvacrol for wound dressing applications<br>Mr. Enrique Gamez Herrera, Dr. Silvia Irusta, Dr. Manuel Arruebo</td>
      <td>190</td>
    </tr>
    <tr>
      <td>Evaluation of Epigenetic Changes in Repetitive Sequences of Human DNA Inducted by Nanoparticles: A Pilot Study for Nanoparticle-Epigenomics Interaction<br>Dr. Amornpun Sereemaspun, Ms. Siwaporn Nilyai</td>
      <td>192</td>
    </tr>
    <tr>
      <td>Niosome nanoparticles loaded with essential oils for wound dressing applications<br>Mrs. Sara García Salinas, Mrs. Hellen Elizondo, Dr. Víctor Sebastián, Dr. Manuel Arruebo, Dr. Silvia Irusta, Dr. Gracia Mendoza</td>
      <td>194</td>
    </tr>
    <tr>
      <td>Simple and Efficient Approach for siRNA Encapsulation into PCLs by Freeze-thawing Method<br>Ms. Ayaka Okamoto, Dr. Hiroyuki Koide, Mr. Hiroki Tsuchida, Dr. Hidenori Ando, Ms. Saki Ariizumi, Ms. Chiaki Kiyokawa, Mr. Masahiro Hashimoto, Dr. Tomohiro Asai, Dr. Takehisa Dewa, Prof. Naoto Oku</td>
      <td>196</td>
    </tr>
    <tr>
      <td>Adsorption of Bilirubin by Chitosan Coated Activated Carbon Prepared from Date Pits<br>Ms. Ameera Seyedzadeh, Ms. Asil Mwafy</td>
      <td>197</td>
    </tr>
    <tr>
      <td>Using MEMS Resonant Mass Measurement to Characterize Mass, Density and Count of Nano-scale Particles<br>Dr. Hanna Jankevics Jones, Mrs. Rachel Bott, Mr. Stephen Carrington, Dr. Matthew Barea</td>
      <td>198</td>
    </tr>
    <tr>
      <td>Polydopamine, a potential mucopenetrative nanomaterial capable of multimodal therapy for bladder cancer<br>Ms. Barbara Poinard, Mr. Samuel Neo, Ms. Angeline Tan, Mr. Roy Tan, Prof. Koon Gee Neoh, Dr. James Kah</td>
      <td>199</td>
    </tr>
  </tbody>
</table>
<table>
  <tbody>
    <tr>
      <td>Development of a Long-circulating Liposomal Carrier Coated with Serum Albumin via Ligand<br>Ms. Hikari Sato, Mr. Yuta Nakamura, Prof. Akihiro Kishimura, Prof. Takeshi Mori, Prof. Yoshiki Katayama</td>
      <td>200</td>
    </tr>
    <tr>
      <td>CNTs recruit different serum protein assortments on the biocorona<br>Mrs. ESPERANZA PADÍN GONZÁLEZ, Mrs. Nerea Iturrioz, Mrs. Eloisa González-lavado, Ms. Carmen Pesquera, Mr. Fernando González, Ms. Monica L. Fanarraga</td>
      <td>202</td>
    </tr>
    <tr>
      <td>In vitro correction of congenital disorder of glycosylation type Ia (CDG Ia) using PLGA nanoparticles loaded with GDP-Man.<br>Dr. Barbara Bortot, Dr. Eleonora De Martino, Dr. Alessandra Tesser, Prof. Giovanni Tosi, Prof. Barbara Ruozi, Dr. Giovanni Maria Severini</td>
      <td>204</td>
    </tr>
    <tr>
      <td>Effect of the nanopartilcle shape on the cancer treatment: the case of pluronic F127 stabilized and doxorubicin loaded magnetite nanocubes and nanospheres.<br>Dr. Timur Nizamov, Dr. Anastasia Garanina, Prof. Alexander Savchenko, Prof. Alexander Majouga</td>
      <td>206</td>
    </tr>
    <tr>
      <td>Atomic Force Acoustic Microscopy for the Characterization of Gold Nanoparticles Embedded into a Polymeric Matrix and its Promissory Application in Nanomedicine<br>Dr. Daniela Geraldo, Mr. Eduardo Jeraldo, Prof. Ramiro Arratia-perez</td>
      <td>207</td>
    </tr>
    <tr>
      <td>Preparation and characterization of multimeric system of RGD-grafted PMMA-nanoparticles as a targeted drug-delivery system for paclitaxel<br>Ms. Brenda Vianey Gibbens-Bandala, Dr. Enrique Morales-Avila, Dr. Blanca Eli Ocampo-García, Dr. Guillermina Ferro-Flores</td>
      <td>209</td>
    </tr>
    <tr>
      <td>Effect of Ligand Shell Composition on Biodistribution of Ultrasmall Gold Nanoparticles<br>Dr. Yao Ding, Dr. Tom Coulter, Ms. Cristina Espinosa Garcia, Dr. Sarah Hale, Mr. Alessandro Pace, Dr. Ketan Patel, Ms. Usoa Aguilera Peral, Mrs. Angela Robinson, Dr. Dan Palmer, Dr. Phil Williams, Dr. Meike Roskamp</td>
      <td>211</td>
    </tr>
    <tr>
      <td>Influence of biophysical anomalies on forest tree quality<br>Dr. Aigars Indriksons, Mr. Maris Eglite, Dr. Imants Liepa</td>
      <td>213</td>
    </tr>
    <tr>
      <td>Engineered Nanoparticles for Rapid Delivery of Betulinic Acid in MDA MB 231 and HEp-2 Cancer Cell Lines<br>Mr. Asim Halder, Ms. Pritha Mukherjee, Mrs. Subarna Ghosh, Dr. Urmi Chatterji, Prof. Arup Mukherjee</td>
      <td>214</td>
    </tr>
    <tr>
      <td>Hybrid biocompatible silica nanoparticles as theranostic agent<br>Mr. Ritu Raj, Ms. Carina Crucho, Prof. José Paulo Sequeira Farinha, Prof. Carlos Baleizão</td>
      <td>216</td>
    </tr>
    <tr>
      <td>Comparison of the effect of selenium Nano particles and sodium selenite on the serum and hepatic lipid profiles of rats following experimental exposure to cadmium.<br>Dr. Saeid KarimiDehkordi, Dr. Abdonnaser Mohebbi, Prof. Mohammad Reza Aslani, Dr. Afshin Jafari Dehkordi, Dr. Akram Shahabi</td>
      <td>218</td>
    </tr>
    <tr>
      <td>Mannose Nanoparticles from Guar Gum: Macrophage Vectorization, Drug Delivery and Leishmanicidal Efficacy against Wild and Drug Resistant Strains<br>Prof. Arup Mukherjee, Mr. Asim Halder, Mr. Sumanta Kumar Ghosh, Ms. Nandita Ghosh, Dr. Ena Ray Banerjee</td>
      <td>219</td>
    </tr>
    <tr>
      <td>PAMAM dendrimer curcumin conjugate as a supramolecular polyphenol; a biomimetic approach to improve anticancer properties of curcumin<br>Dr. Vahid Erfani-Moghadam, Prof. Majid Sadeghizadeh, Dr. Alireza Nomani</td>
      <td>221</td>
    </tr>
  </tbody>
</table>
<table>
  <tbody>
    <tr>
      <td>nanoMIL100: a novel efficient tool to target lung cancer<br>Dr. Teresa Simon-Yarza, Dr. Monica Gimenez-marques, Ms. Rhizlaine Mrimi, Ms. Angelika Mielcarek, Dr. Ruxandra Gref, Dr. Patricia Horcajada, Dr. christian serre, Prof. Patrick Couvreur</td>
      <td>222</td>
    </tr>
    <tr>
      <td>Hyperbranched polyglycerol-docetaxel treatments for bladder cancer and the characterization of treated bladder tissue using MALDI-MS imaging<br>Dr. David Plackett, Dr. Clement Mugabe, Ms. Shujun Lin, Dr. Guobin Sun, Dr. Nancy Ford, Dr. Richard Liggins, Prof. Helen Burt</td>
      <td>224</td>
    </tr>
    <tr>
      <td>Albumin as a Nitric Oxide-Traffic Protein: Novel Anticancer Agent<br>Prof. MASAKI OTAGIRI, Dr. Yu Ishima, Prof. Toru Maruyama</td>
      <td>226</td>
    </tr>
    <tr>
      <td>Nanoparticles in proton and heavy ion therapy<br>Dr. erika porcel, Ms. Marta Bolsa, Ms. Daniela Salado, Dr. Lenka Stefancikova, Mr. Vladimir Ivosev, Prof. Sandrine Lacombe</td>
      <td>227</td>
    </tr>
    <tr>
      <td>Frontier biomedical research: from multi-functional bio-interfaces to biomaterials and tissue engineering<br>Prof. Maria Tomoaia-Cotisel</td>
      <td>228</td>
    </tr>
    <tr>
      <td>The Sustainable Release of Vancomycin and Its Degradation Products from Collagen/Hydroxyapatite Nano/Micro Structured Layers Prepared using Different Techniques<br>Dr. Tomas Suchy, Dr. Monika Supova, Dr. Eva Klapkova, Dr. Vaclava Adamkova, Dr. Jan Zavora, Dr. Margit Zaloudkova, Dr. Martin Braun, Dr. Rastislav Ballay, Dr. Frantisek Denk, Dr. Jiri Rebicek, Dr. Marek Pokorny, Dr. Pavla Sauerova, Dr. Marie Hubalek Kalbacova, Dr. Lukas Horny, Dr. Jan Vesely, Mr. Tereza Vonavkova</td>
      <td>229</td>
    </tr>
    <tr>
      <td>Gold Nanoshell-Assisted Wireless Activation of Myotube Contraction<br>Mr. Attilio Marino, Dr. Satoshi Arai, Dr. Yanyan Hou, Prof. Madoka Suzuki, Prof. Gianni Ciofani</td>
      <td>231</td>
    </tr>
    <tr>
      <td>Complete 3D regeneration of functional organs by manipulation of the pluripotent stem cells<br>Prof. Hiroshi Kagami</td>
      <td>232</td>
    </tr>
    <tr>
      <td>Evaluation of theranostic dendrimers for radiotherapy and MRI of gliomas<br>Ms. Flonja Liko, Prof. Eduardo Fernandez-Megia, Prof. Francois Hindré</td>
      <td>233</td>
    </tr>
    <tr>
      <td>Targeting Hypoxia in Advanced Prostate Cancer Using Tirapazamine-Copper Nanoparticles<br>Dr. Wafa Al-Jamal</td>
      <td>235</td>
    </tr>
    <tr>
      <td>Theoretical Reactivity of Cell-Surface Receptors in Presence of Nanodiamonds as Carriers<br>Dr. Norma Flores-Holguin, Prof. Linda-Lucila Landeros-Martinez, Dr. Erasmo Orrantia-Borunda</td>
      <td>236</td>
    </tr>
    <tr>
      <td>Photocatalytic TiO2 Nanoparticles for Tumor Therapy<br>Ms. Susanne Koch, Dr. Sofia Dembski, Dr. Stephan Hackenberg, Dr. Karine Heuzé</td>
      <td>238</td>
    </tr>
    <tr>
      <td>Immobilized ERK2 and GSK-3beta magnetic microparticles for targeted protein phosphorylation<br>Dr. Marcela Slovakova, Ms. Lenka Hromádková, Mr. Rudolf Kupčík, Dr. Daniela Řípová, Prof. Zuzana Bílková</td>
      <td>240</td>
    </tr>
    <tr>
      <td>Evaluation of magnetic nanoparticles coated by Taxol imprinted polymer for controlled drug delivery in mouse breast cancer model<br>Prof. Hamid Hashemi-Moghaddam, Mrs. Elnaz Mirsaeed-Gazi, Prof. Saeed Zavareh</td>
      <td>241</td>
    </tr>
    <tr>
      <td>Enhanced antifungal activity of itraconazole by the supercritical antisolvent technique<br>Dr. Jayvadan Patel</td>
      <td>242</td>
    </tr>
  </tbody>
</table>
**WORKSHOP: Synthesis of amphiphilic hyaluronan containing-phenyl fatty acids for the preparation of polymeric micelles for applications in drug delivery** 243

Dr. Gloria Huerta-Angeles, Ms. Alena Matelova, Mrs. Zdislava Brunová, Dr. Daniela Šmejkalová, Prof. Vladímir Velebný

**Effects of nanoparticles on gastrointestinal disorders and therapy** 244

Ms. Rabia Riasat, Prof. Nie Guangjun, Ms. Zertashia Tariq

**Synthesis and characterization of biocompatible, non-toxic dopamine coated novel flower shapes core (Fe)/ porous hollow shell (Fe3O4) super paramagnetic Fe/Fe3O4 nanoparticles** 245

Ms. Rabia Riasat, Prof. Nie Guangjun, Ms. Zertashia Tariq, Ms. Muntaha Sakeena

**Competitive intelligence study of a non-viral vector for gene therapy of Parkinson's disease (Cinvestav-PX-001)** 247

Dr. AMERICA PADILLA, Dr. Daniel Martínez-Fong, Dr. Victor Manuel Téllez-López, Dr. Armando De Jesús Espadas-Álvarez
Theragnostic Nanoparticles for Drug Delivery Systems

Wednesday, 28th September - 09:05 - Plenary Speeches - Amphitheatre 25 - Oral presentation - Abstract
ID: 564

Prof. Ick Chan Kwon ¹

1. Korea Institute of Science and Technology

Recently, nanoparticles have received a great interest an application for diagnosis and therapy. Since nanoparti-
cles possess intrinsic features that are often required for drug delivery system and diagnosis, they have potential
as platforms for integrating imaging and therapeutic functions, simultaneously. Especially, molecular imaging
with theragnostic nanoparticles makes it possible not only to provide useful information for monitoring drug
delivery, drug release, and therapeutic efficacy of drug, but also to determine whether the patients are likely to
respond to a therapy. To achieve these goals, a variety of imaging techniques have been used, including near-
infrared fluorescent (NIRF) imaging, magnetic resonance imaging (MRI) and nuclear imaging (SPECT and PET).
Imaging and monitoring of nanoparticles after systemically administered in living systems play key roles in the
development of theragnostic nanoparticles to optimize their physicochemical properties. It has become clear
that imaging drug delivery can assist in analyzing the drug delivery and in predicting the therapeutic efficacy
of cancer-targeted nanoparticles. This presentation will highlight our recent advances that have been made in
the development of multifunctional nanoparticles and the applications of these nanoparticles into theragnostic
nanomedicine.
# Magnetic nanoparticles in cell therapies: from combined approaches in cancer treatment to magnetic tissue engineering.

Wednesday, 28th September - 09:40 - Plenary Speeches - Amphitheatre 25 - Oral presentation - Abstract
ID: 551

## Prof. Claire Wilhelm¹

1. CNRS and Paris Diderot University, laboratory Matière et Systèmes Complexes.

To overcome some of the limitations of current cell therapies tools, new strategies have emerged since the advent of nanotechnology in medicine.

In cancer therapy, thermal treatments (magnetic hyperthermia or photothermal therapy mediated by magnetic or plasmonic nanoparticles) have provided noninvasive means of heating cells at therapeutic levels. However, while the ultimate target for nanoparticle-mediated photothermal therapy is the cancer cell, heating performance has not previously been evaluated inside the cells. In the attempt to bridge this gap, we provided the first thermal measurements mediated by magnetic [1] or plasmonic [2] nanoparticles inside cancer cells, in vitro or in vivo in the tumor environment. The ultimate goal of nanotherapies is anyway to improve the efficacy and combat the tumour from within. We proposed combined nanotherapeutic concepts [3-6] based on magnetothermal, photothermal, and photodynamic therapies which led to complete cancer cell destruction in vitro and complete tumor ablation in vivo.

While magnetic nanoparticles are increasingly used as clinical agents for imaging and therapy, their use as a tool for tissue engineering opens up challenging perspectives that have rarely been explored. Our strategy has been to take advantage of magnetic nanoparticles internalization to create thick, organized, purely cellular 3D tissue structures [7,8], that can be stimulated on demand [9].

The use of nanoparticles for cancer cell therapies or tissue engineering raise more general issues of nanoparticles biosafety, once internalized in cells. Yet the nanoparticles long-term tissular fate is poorly documented. We have developed original magnetic techniques to follow the fate of iron oxide nanoparticles and their assimilation within a living tissue, and evidenced that a massive biodegradation can occur at the endosome site.

[1] Biomaterials 24, 6400-6411 (2014)

[2] Advanced HealthCare Materials, 10.1002/adhm.201501035 (2016)

[3] ACS Nano, 10, 2436-46 (2016)

[4] Nanoscale, 7, 18872-18877 (2015)

[5] Nanomedicine. 10, 2797 (2015)

[6] ACS Nano, 9, 2904-2916 (2015)

[7] Advanced Materials. 25, 2611-2616 (2013)

[8] Integrative Biology, 7, 170-177 (2015)

[9] Phys Rev Lett, 114, 098105 (2015).
# Nano-antibiotics: A rational design of functional nanoparticles to combat bacterial infection

Wednesday, 28th September - 10:45 - Plenary Speeches - Amphitheatre 25 - Oral presentation - Abstract
ID: 542

**Dr. Rabah Boukherroub** ¹

1. CNRS, Lille University

Complications related to infectious diseases have significantly reduced, particularly in the developed countries, due to the availability and use of a wide variety of antibiotics and antimicrobial agents. However, excessive use of antibiotics and antimicrobial agents increased the number of drug resistant pathogens, and this has resulted in a significant threat to public health. The inexorable rise in the incidence of antibiotic resistance in bacterial pathogens, coupled with the low rate of emergence of new clinically useful antibiotics, has refocused attention on finding alternatives to overcome antimicrobial resistance. Novel strategies aiming to reduce the amount of antibiotics, but able to prevent and treat animal and human infections should be investigated, evidenced and approved.

Among the various approaches, the use of nanotechnology (engineered nanoparticles) is currently the most promising strategy to overcome microbial drug resistance. Due to their small size, nanoparticles can surmount existing drug resistance mechanisms, including decreased uptake and increased efflux of drug from the microbial cell, biofilm formation, and intracellular bacteria. Moreover, loading multiple antimicrobial agents on the same nanoparticle makes the development of resistance unlikely. Finally, nanoparticles can target antimicrobial agents to the site of infection, so that higher doses of drug are given at the infected site, thereby overcoming resistance.

Despite considerable recent progress in the understanding of the mechanisms underlying bacterial infections, and in the development of nanostructured materials displaying antibacterial properties and activity against biofilms, the quest to design and fabricate new antibacterial nanostructures remains a high research priority. In this presentation, after an overview on the different nanomaterials possessing antimicrobial activities, I will discuss the use of nanosized drug carriers to efficiently administer antibiotics by improving their pharmacokinetics and bioaccumulation, while reducing the adverse effects of antibiotics.
# Considerations on the design of nanoparticles for diagnostic, theranostic and therapeutic purposes

Wednesday, 28th September - 11:25 - Plenary Speeches - Amphitheatre 25 - Oral presentation - Abstract
ID: 524

Prof. Fabian Kiessling¹

1. RWTH Aachen University

Nanoparticles are frequently suggested for medical use. However, often basic considerations on its pharmacokinetic properties are not taken into account. Additionally, its optimal characteristics have to be very different if they are considered for diagnostic or therapeutic purposes.

Nanoparticles >5 nm tend to be removed by the RES. Thus, tissues belonging to the RES like liver spleen and lymph nodes can be targeted with such nanoparticles. Furthermore, if phagocyting cells migrate to pathological sites, the nanoparticles will also be accumulated, which has been shown e.g. for (U)SPIO. Phagocyting cells can also be labelled ex vivo with diagnostic nanoparticles, which opens great perspectives for cell tracking and the imaging of tissue engineered transplants.

Adding stealth properties to the nanoparticles increases their circulation time giving them more time to ex-
travasate in tissue with high vessel permeability. This so called EPR based accumulation is the basis for most
tumor targeted nanomedicines. However, the therapeutic benefit over small probes is often only moderate
since EPR is variable among patients and even heterogeneous within the same tumor. Here, theranostic agents
and companion diagnostics can help to preselect patients and to individualize therapy. In addition, in tumors
larger nanoparticles tend to accumulate just outside the vasculature, do hardly penetrate the stroma and thus
do not reach the cancer cells. Thus, a refined balance between accumulation and penetration may be thera-
peutically superior over just maximal accumulation. In this context, active targeting does only marginally help
since it does not improve nanoparticle distribution and accumulation but just retention. Active targeting be-
comes more evident for small nanoparticles (< 5 nm) showing good penetration and rapid exchange between
the tissue compartments but insufficient retention. Additionally, these are the ones that are most suited for
molecular imaging purposes as well.

Thus, the intended medical application should route the decisions about design of nanoparticles and all aspects
relevant to its in vivo application including the expected superiority over existing clinical gold standards should
be considered from beginning on. Following this conduct, many failures in the transition from in vitro to in vivo
application can be avoided.
Chitosan-Collagen hybrid 3D-scaffolds as potential
biomaterials for tissue engineering

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 81

Ms. Hilary Urena ¹, Dr. Marianelly Esquivel-Alfaro ², Dr. Sergio Madrigal-Carballo ¹

1. National Center for Biotechnological Innovations (CENIBiot), CeNAT-CONARE, 2. Polymers Research Laboratory (POLIUNA), National University

Introduction. Chitosan has been applied to promote extracellular matrix (ECM) formation in tissue regenerative therapy. The superior tissue compatibility of chitosan may primarily be attributed to its structural similarity to glycosaminoglycan in ECM. Chitosan has been reported to be biocompatible, bio-absorbable and particu- larly, is considered a good wound-healing accelerator. Dermis and scaffolds made from chitosan exhibit weak antigenicity, biodegradability, and superior biocompatibility (hemostatic and cell-binding properties) by com- parison to the synthetic polymers, such as poly(lactic acid) (PLA), poly(glycolic acid) (PGA), and polyethylene terephthalate (PET). As a scaffold, chitosan-based materials in the form of a sponge have been considered the most popular 3D-scaffolds for dermal regeneration. Of the many scaffold materials being investigated, colla- gen has been shown to have many advantageous features. Highly porous collagen lattice sponges have been used to support in vitro growth of many types of tissues. Methods. We isolated chitosan from native shrimp waste streams and collagen from tilapia aquaculture waste by-products. Hybrid 3D-scaffold biomaterials were successfully obtained by mixing chitosan with collagen at different molar ratios. Chitosan-collagen hybrid com- posites were formulated as 3D sponge-like scaffolds, applying previously developed methodologies involving solvent casting and freeze-drying. Results. Chitosan-collagen hybrid 3D-scaffolds were characterized accord- ing to its water uptake capacity, thermal behavior (DSC) and morphology (SEM). Discussion. Chitosan-collagen hybrid 3D-scaffolds showed improved stability, higher active compound loading capacity, better release prop- erties, improved cell uptake, greater porosity, tensile strength and increased thermal stability as compared to current synthetic scaffolds. These hybrid nanostructured biomaterials will be suitable for the incorporation of active ingredients that may potentiate its application as dressings, cell culture scaffolds We are currently starting cell growth studies on the scaffolds using model epithelial cells.
<figure><img src="image_7.png" /><figcaption>Cht-cgn scaffolds.jpg</figcaption></figure>
# Corona interactome: a key for deciphering protein adsorption kinetics on silica nanocarriers

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 554

**Mr. Cédric Pisani**¹, **Mr. Jean-charles Gaillard**², **Mr. Michaël Odorico**³, **Dr. Jeff Nyalosaso**¹, **Dr. Clarence Charnay**¹, **Dr. Yannick Guari**¹, **Prof. Joël Chopineau**¹, **Prof. Jean-marie Devoisselle**¹, **Dr. Jean Armengaud**², **Dr. Odette Prat**⁴

1. Université de Montpellier, 2. CEA, IBITECS, 3. CEA, ICSM, 4. CEA, BIAM

Magnetic mesoporous silica nanoparticles (M-MSNs) represent promising targeting tools for cancer diagnostic and therapy. In biological systems, nanoparticles interact with proteins and form a layer named "corona" that drives their biological fate and toxicity. The corona around NP creates a new nano-object, whose interactions with living cells are different from those induced by the pristine NPs. We have investigated the behavior of adsorbed proteins around M-MSNs with two biological fluids, fetal bovine and human sera. The first one is interesting for in vitro toxicology and the second for in vivo diagnosis and therapy. Thereby, after a quantification of protein adsorption on these M-MSNs by BCA assay over time, we qualified these adsorbed proteins by high throughput comparative proteomics during long term kinetics. We observed an increase of the protein layer with both serum types from 30 seconds to 7 days of contact by both techniques. During this kinetics, the growth of the protein corona began instantly after NP immersion. We thus detected 90 to 128 and 134 to 153 distinct adsorbed proteins for the bovine and human corona, respectively. By using computational biology tools and protein-protein interaction databases, we have highlighted three major clusters of protein behavior in contact with nanoparticles. We demonstrated precisely how the protein network builds up within the corona during the experimental kinetics resulting in a multiparametric representation of the "corona interactome". This original approach may allow understanding how NPs interact with biological fluids and provide some clues for the design of stealth nanocarriers.
# Design of mesoporous Fe3O4/MS nanoparticles as drug delivery platform of prednisolone

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 88

Mrs. Ivalina Trendafilova¹, Prof. Margarita Popova², Prof. Agnes Szegedi³, Prof. Judith Mihály³, Prof. Denitsa Momekova⁴, Prof. Georgi Momekov⁴, Dr. Irina Nikolova⁴, Mr. Lyubomir Marinov⁴

1. Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, 2. Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, 3. Research Centre for Natural Sciences, Institute of Materials and Environmental Chemistry, Hungarian Academy of Sciences, 1117 Budapest, 4. Faculty of Pharmacy, Medical University of Sofia

Interest towards mesoporous silicates as drug carriers goes back to 2001, when application of MCM-41 type mesoporous silica for controlled delivery of drugs was published for the first time. Mesoporous silicates (MS) are characterized by narrow distribution of pores with a controlled size, large pore volume, high specific surface area (≤700 m2/g), and good chemical and thermal stability. A new push towards the development of antitumor drug-delivery systems is given by the Fe3O4/MS mesoporous composites with different structures. By integration of mesoporous silica nanostructures with magnetic nanocrystals, original drug-delivery systems can be obtained permitting selective supply of the drug to the targeted organ or tissue of the body. Glucocorticoids i.e. prednisolone have been used in clinical oncology for more than three decades because their anti-inflammatory action additionally influences the oncological therapy.

In the present study we developed prednisolone loaded Fe3O4/MS nanoparticles. In-vitro release properties of the obtained delivery systems were studied in respect to their possible application in anti-cancer therapy. Fe3O4/MS nanoparticles with spherical morphology, small particle sizes (80 nm) and high surface area (≤800 m2/g) were synthesized and loaded with prednisolone by incipient wetness impregnation. The Fe3O4/MS nanoparticles and drug formulations were characterized by XRD, N2 physisorption, TG analysis and ATR-FT-IR spectroscopy. In-vitro drug release study was performed into phosphate buffer (pH = 7) at 37oC. Loading of prednisolone on Fe3O4/MS nanoparticles resulted in high loading capacities. In-vitro release process at showed controlled prednisolone release. Spectroscopic data suggest the formation of a weak bond between silanols of the mesoporous Fe3O4/MS nanoparticles and prednisolone molecules. The cytotoxic potential of the presented mesoporous nanoparticles was tested in a panel of human malignant cell lines in-vitro. The results clearly indicate that the nanoparticles are practically devoid of toxicity in the tested concentration range. The in-vivo anti-inflammatory activity of prednisolone loaded mesoporous Fe3O4/MS nanoparticles vs. free drug was compared in murine collagen-induces arthritis. The results show that prednisolone encapsulation into mesoporous nanoparticles did not compromise its intrinsic pharmacological activity.

Acknowledgements Financial support from the Bulgarian-Hungarian Inter-Academic Exchange Agreement and the program for career development of young scientists, BAS, ДФНП 191/14.05.2016 is greatly acknowledged.
<figure><img src="image_8.png" /><figcaption>Abstr.jpg</figcaption></figure>
# Cytotoxic Evaluation of Irinotecan-loaded PLGA-PEG-retinoic acid Nanomicelles on HT-29 Cancer Cell Line

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 218

Dr. Parnian Maghzi¹, Dr. Jaber Emami¹, Dr. Farshid Hasanzadeh¹, Dr. Hojjat Sadeghi¹

1. isfahan university of medical science

In the present study PLGA-PEG and PLGA-PEG-retinoic acid (RA) which had already been synthesized in our laboratory were used to prepare targeted (PLGA-PEG-RA) and non-targeted (PLGA-PEG) polymeric micelles by thin-film hydration method to target irinotecan to colorectal cancer cell (HT-29). Cytotoxic effect of irinotecan solution, non-targeted irinotecan-loaded nanomicelles, targeted irinotecan-loaded nanomicelles, and blank nanomicelles were evaluated on HT-29 cell line using MTT assay. Untreated cells were taken as the negative control and the blank culture medium was used as the control. Cells were grown in RPMI 1640 medium and incubated with samples for 72 h at the equivalent irinotecan concentrations of 1 to 8 µg/ml. Cell viability for each sample was then calculated. Blank micelles did not show any measurable toxicity on HT-29 cell line indicating the safety of PLGA-PEG-RA as a potential drug carrier for irinotecan to the tumor cells. Toxicity of irinotecan and irinotecan-loaded nanomicelles were significantly increased in a drug concentration dependent manner. Non-targeted irinotecan-loaded nanomicelles showed greater cytotoxicity than free irinotecan while the cell toxicity of targeted irinotecan-loaded nanomicelles was significantly greater than both free irinotecan and non-targeted irinotecan-loaded nanomicelles. IC50 values of drug-loaded PLGA-PEG and drug-loaded PLGA-PEG-RA nanomicelles were significantly lower than those of the free drug. Irinotecan-loaded targeted nanomicelles exhibited lowest IC50 values as compared to free irinotecan and non-targeted nanomicelles. More cytotoxicity of non-targeted nanomicelles compared to free drug might be due to the presence of PEG on the surface of nanomicelles forming a brush-like shell that stretches away from the core reducing micelle aggregations and increasing cellular uptake. Drug-loaded PLGA-PEG-RA nanomicelles indicated highest toxicity compared to non-targeted nanomicelles which could be attributed to the receptor-mediated endocytosis and the nucleus-directed drug targeting of the nanoparticles. Our results indicate that the RA-conjugated nanomicelles could have great potential for targeted therapy of irinotecan, may reduce irinotecan therapeutic dose and consequently reduces adverse effects of this drug.
<figure><img src="image_9.png" /><figcaption>Fig. ht-29 cell survival.jpg</figcaption></figure>
PREPARATION, CHARACTERIZATION AND
RADIOLABELED OF VENLAFAXINE-PLGA
NANOPARTICLES WITH 99mTC FOR BRAIN DELIVERY

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 115

Ms. María Dolores Cayero ¹, Ms. ROCÍO IGLESIAS JEREZ ², Mr. Roque Salazar Cabrera ³, Ms. Isabel Borrego Dorado ², Dr. Lucía Martín-Banderas ¹

1. Pharmacy and Pharmaceutical Technology, University of Sevilla, 2. Servicio de Medicina Nuclear. Hospital Universitario Virgen del Rocío, 3. IBA Molecular

Introduction: Crossing the BBB is a major challenge for the efficient delivery in the brain. Fortunately, exhibits transport mechanisms and there are numerous strategies to cross it. Transferrin (Tf) has been widely applied to enhance cellular uptake of nanoparticles (NPs) in brain delivery.

To evaluate the biodistribution of nanocarriers, NPs was radiolabeled with radioisotopes for evaluation in organs or tissues after administration.

Methods: NPs were prepared by emulsion solvent evaporation technique. The size and zeta potential (ZP) was measured and the morphology and shape were examined by using transmission electron microscope. The encapsulation efficiency were determined indirectly by HPLC.

After, Tf was conjugated on the surface of NPs using a carbodiimide method with EDC and NHS. Conjugation efficiency was measured indirectly.

For radiolabeled, lyophilized NPs were dispersed in NaCl in vials under vacuum. SnCl2-2-hydrate were dissolved in HCl 37% and was added with ≈74 MBq of 99mTc. Finally, the system was incubated for 10 min.

99mTc-NPs suspensions were analyzed by thin layer chromatography (TLC) with silica gel strips. With 0.9% NaCl as the mobile phase, free pertechnetate ran with the front, meanwhile particles stayed in the start. Using a solution of pyridine:acetic acid:water (3:5:15), radiocolloids remained at start, NPs migrated with Rf = 0.6-0.8.

Results: The particle size, polydispersity index (PdI) and ZP of NPs were characterized by DSC. NPs had a mean size of 253.02±13.10 nm with a zeta potential of -3.30±1.74 mV. Their PdI of 0.043±0.03. After conjugation, NPs-Tf showed a mean size of 312.94±27.93 nm with a PdI less than 0.2 and a ZP of -2.40±0.56mV. The NPs had an average encapsulation efficiency of up to 65.5±2.65% and the conjugation efficiency was 60.4±3.77. T.E.M. analysis revealed that NPs had a solid structure and spherical shape.

PLGA nanoparticles with Tf and without surface modification were labeled with 99mTc with a yield ≥ 90 % (TLC). After radiolabeling, particles increased in size, ≈ 380 nm.

Discussion: NPs exhibits a suitable size, a high homogeneity, encapsulation and conjugation efficiency.

Radiolabeling process were reproducible and nanosystem is suitable for in vivo theragnosis.
Capsaicin-Coated silver nanoparticles inhibit amyloid fibril formation of serum albumin

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 197

Mr. Bibin G Anand ¹, Dr. Karunakar Kar ¹, Mrs. Kriti Dubey ¹, Mr. Dolat Singh shekhawat ¹

1. Indian Institute of technology, Jodhpur

Here, we have synthesized capsaicin-coated silver nanoparticles (AgNPsCap) and have tested their anti-amyloid activity, considering serum albumin (BSA) as a model protein. We found that amyloid formation of BSA was strongly suppressed in the presence of AgNPsCap nanoparticles. However, isolated capsaicin and uncapped control nanoparticles did not show such inhibition effect. Bioinformatics analysis reveals CH-Δ and H-bonding interactions between capsaicin and BSA in the formation of protein-ligand complex. These results suggest the significance of surface functionalization of nanoparticles with capsaicin which probably enables capsaicin to effectively interact with the key residues of the amyloidogenic core of BSA.
# Diamond micro-cantilevers as transducers for Olfactory Receptors - based biosensors

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 238

Dr. Raafa Manai¹, Dr. Dounia Kamouni-Belghiti¹, Dr. Marie-Annick Persuy², Ms. Massiel Habchi¹, Dr. Lionel Rousseau³, Ms. Maira Possas Abreu³, Dr. Philippe Bergonzo¹, Dr. Edith Pajot-Augy², Dr. Guenhaël Sanz², Dr. Emmanuel Scorsone¹

1. CEA, 2. INRA, 3. ESIEE

## Introduction

Olfactory receptors (ORs) are proteins located in the plasma membrane of olfactory sensory neurons. They belong to the family of G protein-coupled receptors. Individual ORs bind various odorants with distinct affinities and specificities. Therefore, ORs combined into arrays are considered as highly promising for the conception of biosensors for odorant detection. Here we investigated a new approach consisting of chemically grafting the ORs onto synthetic diamond grown by Chemical Vapor Deposition. Because of its stability, biocompatibility, and ability to immobilize biological targets, diamond is a material of choice for biosensors.

## Methods

ORs were grafted onto diamond surfaces using two routes, either (1) through covalent bonding of hexanoic acid radical on diamond followed by EDC/NHS peptidic coupling to an OR, or (2) using covalent attachment of nitriloacetic acid (NTA) on diamond as chelating agent, which binds an 6His-tagged OR via NTA-Ni interaction. Both grafting procedures were validated by electrochemical impedance spectroscopy (EIS) on boron doped diamond electrodes. They were then applied to bulk diamond micro-cantilevers freshly hydrogenated in hydrogen plasma in a Chemical Vapour Deposition reactor. Functionality for odorant detection was assessed in the liquid phase using a Laser Doppler read-out system.

## Results

OR7D4 was grafted through route (1), while 6His-tagged M71 was grafted through route (2). The Nyquist plots obtained from both EIS spectra exhibit an increase of the charge-transfer resistance, thus of the electrode coverage, showing for the first time an efficient immobilization of ORs on diamond.

Then, the 6His-M71 micro-cantilever sensor exhibited a good sensitivity to its known odorant ligand acetophenone, with a frequency shift near 100Hz for 1µM exposure (320Hz for 10µM), and a good selectivity against a non-ligand odorant. Likewise, the OR7D4 micro-cantilever sensor showed a sensitivity of 200Hz for exposure to its ligand androstenone at 1µM with a good selectivity against non-ligand odorants.

## Discussion

Micro-cantilevers grafted with ORs are thus functional, and yield a quantitative, reproducible response to target analytes, at least at the micromolar level, and selectivity among odorants. The results are promising for the development of bioelectronic noses consisting of arrays of OR-grafted diamond-based resonators for specifically detecting target odorants.
<figure><img src="image_10.png" /><figcaption>Typical response of 6His-M71 based micro-cantilever</figcaption></figure>

Figure iconan.jpg
# Diphtheria toxin-derived protein for targeted delivery of nanoparticles

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 243

Mr. Mahesh Agarwal¹, Dr. Amaresh Sahoo², Dr. Biplab Bose¹

1. Indian Institute of technology, Guwahati, 2. Indian Institute of Information technology, Allahabad

Over the last one decade, various types of nano-formulations have been developed and approved for use. However, cell-specific delivery of nanoparticles (NPs) is still a challenge. Antibodies and peptides are often used for targeted delivery of NPs. In the present work, we have used a small protein derived from Diphtheria toxin to home NPs to specific cells. Diphtheria toxin (DT) binds to a specific receptor on cell surface and is internalized by receptor-mediated endocytosis. This receptor is overexpressed in various types of cancer cells. We have expressed the recombinant receptor-binding domain of DT (RDT) that binds to the cell surface receptor. We have coated PLGA NPs with RDT. Coating, with RDT, is revealed by UV spectroscopy and Bradford's assay. RDT coated NPs showed enhanced cellular uptake of NPs in U-87 MG cells with specific DT receptor. The internalization of RDT coated NPs is shown by Flow cytometer and Fluorescent microscope. These RDT-coated NPs can be used for delivery of a chemotherapeutic agent to cells expressing DT-receptors.
Formulation and In-Vitro Evaluation of Enteric Coated
Capsules Filled with Recombinant Human Keratinocyte
Growth Factor Loaded Chitosan Nanoparticles

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 245

Dr. Palanirajan Vijayaraj Kumar ¹, Dr. Yeong Siew Wei ¹, Mr. Mhd Luay Takahje ¹, Dr. Lee Ming Tatt ¹,
Prof. Abu Bakar Bin Abdul Majeed ², Dr. Vasudevan Mani ²

1. UCSI University, Faculty of Pharmacy, No. 1, Jalan Menara Gading, Taman Connaught, Cheras 56000, Kuala Lumpur, 2. Universiti Teknologi MARA, Faculty of Pharmacy, Research Management Institute, Shah Alam

Recombinant human keratinocyte growth factor (rHuKGF) is a protein used to treat oral mucositis, small intesti-
nal ulceration and colitis in hematological malignancies patients who are receiving radio and chemotherapy.
Mucoadhesive chitosan nanoparticles are used to develop oral delivery systems. In this project, rHuKGF loaded
chitosan nanoparticles were prepared by ionotropic gelation of chitosan with tripolyphosphate anions with the
presence and absence of PEG 2000 as a cryo-protectant. Size distribution and zeta potential of rHuKGF loaded
chitosan nanoparticles were determined by using zeta-sizer. In addition, the morphology was determined by
using field emission scanning electron microscope. Enzyme linked immunosorbent assay was used to deter-
mine the amount of rHuKGF that was loaded in chitosan nanoparticles. The prepared chitosan nanoparticles
were filled in capsules and coated by enteric coating polymer kollicoat MAE 100 P, in-vitro dissolution stud-
ies were performed to evaluate the release of rHuKGF in a buffer solution having pH of the small intestine by
using USP dissolution apparatus I. The rHuKGF loaded chitosan nanoparticles with and without PEG 2000 had
spherical shape and positive surface charge. Particle size distribution of rHuKGF loaded chitosan nanoparticles
was ranged from 119 to 385.7 nm. The rHuKGF loaded chitosan nanoparticles prepared with PEG 2000 capable
to re-disperse fast without forming aggregates in simulated intestinal fluid pH 5.5 phosphate buffer solution.
In-vitro release study showed the ability of kolicoat MAE 100 P to prevent the release of rHuKGF in simulated
gastric fluid pH 1.2 and allow it to release in simulated phosphate buffer solutions pH higher than 5.5.
<figure><img src="image_11.png" /></figure>

Image shows the scanning electron micrograph of
rhukgf loaded chitosan mucoadhesive nanoparticles
with peg 2000.jpg
# Molecular Imprints for the Detection of Specific Glycoproteins Implicated in Cancer

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 285

Ms. Philippa Mitchell¹, Dr. Lewis Hart¹, Prof. Paula Mendes¹

1. University of Birmingham

Molecular diagnostics rely heavily on immuno-detection, such as the enzyme-linked immunosorbent assay (ELISA).1 One of the key drawbacks of employing antibodies in biomedical detection systems is their inability to differentiate between different glycoforms of the same protein.2 3 One such ELISA, where this short-coming is pronounced is the prostate specific antigen (PSA) ELISA developed for the diagnosis prostate cancer.4 There are multiple glycoforms of PSA including those that are strongly suggested as biomarkers of prostate cancer, however the ELISA, which is considered the ‘gold standard, cannot distinguish between normal and cancer-ous glycoforms making the test uninformative, unreliable and inaccurate.5 6 To this end, we look to utilise molecular imprinted polymers (MIPs) to produce a novel diagnostic method for the detection of cancerous PSA glycoforms.

MIPs within ultra-thin hydrogels are synthesised from a surface using controlled living polymerisation (Figure 1). An initiator molecule (11'-dithiobis[1-(2-bromo-2-methylpropionyloxy)undecane]) is synthesised and incubated with gold to form a self-assembled monolayer (SAM). In a separate step, PSA is incubated with vinyl-boronic acid to form a complex. The complex is then incubated with the initiator surface and polymerisation triggered around the complex using N,N'-methylenebisacrylamide (MEBA), Cu(I)Br and 2,2'-bipyridine as the metal-ligand catalyst and ethyl-2-bromoisobutyrate as a sacrificial initiator to form an imprint in biologically compatible conditions.

The afforded SAMs were in good agreement with the literature, measuring 1.74 ± 0.12 nm in thickness and with a water advancing angle of 70 ± 1.8°, as evaluated by ellipsometry and contact angle, respectively.7 The extent of the polymer network was determined by the ratio of Cu(I)Br to Cu(II)Br. The resultant system was then optimised to produce MEBA layers with a thickness of 8.07 ± 0.76 nm, which will act as the foundation into which PSA imprints will be formed. We now look to incorporate boronic acid monomers complexed with PSA into the polymerisation network to create MIPs capable of differentiating between the subtle differences in the glycosylation characteristic of each PSA glycoform, to thus produce an informative, reliable and accurate test for prostate cancer.
<figure><img src="image_12.png" /><figcaption>Mip for paris.jpg</figcaption></figure>
# Optimizing liposomes delivering arsenic trioxide to cervical cancer cells in vitro

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 317

Ms. Anam Akhtar¹, Dr. Xuesong Wen¹, Dr. Scarlet Wang¹, Dr. Lucy Ghali¹, Dr. Celia Bell¹

1. Middlesex University

Arsenic trioxide (ATO) has been demonstrated to have significant therapeutic effects against acute promyelocytic leukemia and various haematological malignancies. However the lack of success in clinical trials in treating solid tumours due to its toxicity has prevented its further application. In this study, ATO encapsulated liposomes were prepared and investigated for their physico-chemical characteristics, drug loading efficiency and inhibitory activity on cervical cancer cells under different surface charges (neural, negative or positive charges) and sizes (100, 200 and 400nm). Five different liposomal formulations were prepared by conventional lipid film hydration technique. Liposomes of increasing sizes were prepared by extrusion from filters of 100nm, 200nm and 400nm pore sizes respectively. Charged liposomes were prepared in a similar way by incorporating dimethyldioctadecylammonium bromide (DB) and 1-stearoyl-2-hydroxy-sn-glycero-3-phospho-sodium salt (DSPG) as cationic and anionic charge carrier lipids for liposomes. The resultant liposomes were stable at 4°C with less than 10% arsenic leakage after a month as determined from inductively coupled plasma optical emission spectroscopy (ICP-OES). Loading efficiency was observed to be independent of tested size range; however neutral liposomes had the highest loading efficiency among the charged liposomes. Cellular uptake and apoptosis studies of these liposomes were evaluated against HPV positive (HeLa) and HPV negative (C33a) cervical cancers and a normal control cell line (CRL 1790) through inductively coupled plasma-mass spectroscopy (ICP-MS), flow cytometry and toxicity studies. Liposomal uptake was found to be independent of sizes but dependent on surface charges and cell lines. Positive liposomes displayed the highest uptake, but the highest toxicity to the cells after their exposure rules them out as suitable candidates as drug carriers. Lower toxicity to the cells was observed when liposomal ATO was used instead of free drug and overall, C33a cells were more susceptible to ATO than HeLa despite a lower arsenic uptake. Cancer cells displayed the significant apoptotic effect and toxicity per unit uptake of ATO as opposed to normal cells when liposomal ATO was employed. In conclusion, neutral 100 nm liposomes were chosen as an effective ATO carrier to cervical cancer cells due to their lower toxicity, higher loading efficiency and high stability.
Study Effects of Synchronize Utilize of Gamma-ray and
Silver Nano Particles Treatment on Human Prostate
Cancer Cells

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 329

Dr. alireza shams ¹

1. Alborz University of Medical Sciences, Karaj, Iran

Background: Prostate cancer is the second most common cancer in America which causing great harm and waste cost. Furthermore more prostate cancer treatment is ineffective. Purpose of this study was to evaluate the effects of therapy and the rate of increase of the absorbed dose of gamma radiation with silver nano particles in treatment of prostate cancer.

Materials & Methods: DU145 cell line originating from Human prostate cancer was purchased from Pasteur Institute. After thawing of defreezed samples, cells were incubated with DMEM medium and 15% FBS serum was added. For preventing contamination amphotericin B in 25 µM/ml was added. Cells were incubated over a period of 3 to 5 days to reach a good Con-fluency then cells divided into 4 groups. Tentatively assigned to the control group, the second experimental group treated with gamma irradiation at a doses of 2, 6 and 10 Gray(Gy), the third group treated with 53 µg/ml silver nano particles, The fourth group includes simultaneous treatment with gamma doses and silver nano particle. Cell groups studied by staining with trypan blue as well as by MTT assay (ELISA) reader.

Results: The results showed the use of gamma rays and silver nano particles caused a significant reduction in the number of cancer cells in the treated groups compared to the other treatment groups and a control group. Using of silver nano particles as a radio sensitize and radiation therapy in prostate cancer cell lines DU145 resulted in the increase of the gamma-ray photon energies of 6 and 10 Gy

Discussion: The gamma photon radiation to the tumor cells were incubated nano particle probability Photo-electric process as a process when handling the extremely high energy gamma photons hitting the silver nano particles to cancer cells, photo electron and Auger electrons (Auger Electrons) and secondary electrons produced by secondary particles can be It will not be very effective, leading to failures in the field of DNA damage and cell death.

Keywords: Prostate cancer, gamma - ray, Silver nano particles, DU145, Cell Culture
# RADIOLABELED NANOPARTICLES STRATEGIES FOR BREAST CANCER THERAGNOSIS

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 458

Dr. Lucía Martín-Banderas ¹, Ms. ROCÍO IGLESIAS JEREZ ², Mrs. Mº Dolores Cayero-Otero ³, Dr. Alejandro Perera ⁴, Mr. Roque Salazar Cabrera ⁵, Ms. Isabel Borrego Dorado ²

1. Pharmacy and Pharmaceutical Technology, University of Sevilla, 2. Servicio de Medicina Nuclear. Hospital Universitario Virgen del Rocío, 3. Universidad de Sevilla, 4. Centro de Isotopos (CENTIS). La Habana, 5. IBA Molecular

## Introduction

Nanomedicine offers an exciting new paradigm change for real medicine, which can be translated into a huge impact on health care. This is mainly due to the possibility of combination treatment strategies and diagnostic techniques, known as theragnosis.

## Objective

The objective of present work is to develop double functionalized PLGA nanoparticles for breast cancer theragnosis. PLGA nanoparticles were radiolabeled with 99mTc and particle surface were modified with a monoclonal antibody (MAb) against HER2+ cancer cells.

## Materials and Methods

PLGA nanoparticles were prepared by nanoprecipitation method [1]. Loaded nanoparticles were prepared dissolving paclitaxel (a gift from PhytoBiotech, Canada) into polymer solution in acetone at 10% w/w concentration. Different radiolabeling strategies were carried out [2]:

(i) PLGA nanoparticles were surface modified with a MAb against HER2 + cancer cells using the carbodiimide strategy. Then, nanoparticles were radiolabeled with 99mTc directly using SnCl2.

(ii) Antibody was firstly reduced using a β-mercaptoethanol. For this purpose, MAb was incubated for 30 min at room temperature. After purification (Amicon 30K) to eliminate the excess of β-mercaptoethanol, the reduced antibody was labeled with 99mTc (10 mCi) via Sn2+ reduction of pertechnetate, using sodium pirophospate (Angiocis® 20mg) as a weak competing ligand.

MAb Conjugation Efficiency was evaluated by Bradford method.

## Results and Discussion

We obtained paclitaxel-loaded PLGA nanoparticles 190 nm in diameter with a zeta potential around -20 mV. Encapsulation efficiency was 85% (determined by HPLC). After antibody conjugation particles diameter increases slightly up to 250 nm in diameter. ZP values were nearly neutrality which indicates the presence of MAb on particle surface. Antibody conjugation efficiency was around 90%. That means 30-35 µg of MAb per µg of nanoparticle.

Both radiolabeling strategies were highly effective (up to 98%).

## References

[1] Fessi, H., Puisieux, F., Devissaguet, J.P., Ammoury, N., Benita, S. Int. J. Pharm. (1989) 55, R1-R4

[2] Psimadas D, Bouziotis P, Georgoulias P, Valotassiou V, Tsotakos T, Loudos G. Contrast Media Mol Imaging. (2013), 8(4):333-339
Acknowledgements

Authors are special grateful for the financial support from Fundación Progreso y Salud (Junta de Andalucía) and Janssen Pharmaceuticals. Authors also thanks PhytoBiotech (Canada) for paclitaxel supply
# Mesoporous silica nanoparticles surrounded by a lipid bilayer: Absence of in vitro hemonanotoxicity

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 369

Dr. Christina Grigorakaki ¹, Dr. Vincent Schlesser ², Dr. Céline Hoffmann ¹, Dr. Jean-Sébastien Thomann ³, Ms. Gaëlle Corne ³, Dr. César Pascual Garcia ³, Dr. Sivashankar Krishnamoorthy ³, Prof. Rolf Bjerkvig ¹, Dr. Valérie Palissot ¹

1. Luxembourg Institute of Health, 2. Centre Hospitalier de Luxembourg, 3. Luxembourg Institute of Science and Technology

Nanomedicine aims at rising innovative tools in order to improve the diagnosis and the therapy of human diseases. The development of robust theranostic nanomaterials displaying treatment efficiency and reduction of detrimental side effect to normal tissues will deeply improve patient quality of life. Due to their size and versatile character nanoparticles can pass through the physiological barriers and deliver medicines. Therefore, the translation process of these new therapeutic nanocarriers to their clinical use relies on their interaction with the physiological environment.

Following the synthesis and the complete physicochemical characterization of a 55 nm mesoporous silica nanoparticle surrounded by a lipid bilayer (55 nm MSNP@SLB), we have evaluated its susceptibility for safely intravenous administration. In a first step of in vitro experimentation on red blood cells (RBCs), Scanning Electron Microscopy and hemolysis assay have shown no deformation and disruption of their membrane. No cytotoxic effect was neither observed on Peripheral Blood Mononuclear and on Human Marrow Stromal cells (HS-5 cell line).

We have therefore elaborated our nanoparticle study with the determination of coagulation parameters i.e. Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT). These Nanoparticles display values in a normal range without formation of a clot. These results are in correlation with no platelets activation and aggregation.

Using scanning confocal microscopy, we observed that isolated RBCs that have been in contact with nanoparticles surrounded by a lipid bilayer are not cleared by activated macrophages (THP-1). This phenomenon is correlated with the absence of phosphatidylserine externalization on RBCs membrane. The activated macrophage model incubated with the nanoparticles of interest showed uptake of nanoparticles and did not trigger ROS production.

In conclusion, the nanoparticle under development displays no in vitro toxic effects in the major cellular components of blood and the development of this multiphased nanoplatform should result in a biocompatible theranostic tool.
Hemocompatibility of
55 nm MSNP@SLB

<figure><img src="image_13.png" /></figure>

THP-1 membrane THP-1 nucleus Erythrocyte

Confocal image of activated
THP-1 cells with nanoparticles
pretreated erythrocytes.
# Novel Synthetic Method and Characterization of Magnetic Resonance/Near-infrared dual-modal imaging agents by In Vivo Molecular Imaging

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 372

Mr. Hyunseung Lee¹, Ms. Hyun Min Kim¹, Dr. Jee-Hyun Cho¹, Mr. Jongeun Kang¹, Dr. Hye Sun Park¹, Dr. Kwan Soo Hong¹

1. Bioimaging Research Team, Korea Basic Science Institute

A novel magnetic resonance (MR)/near-infrared (NIR) imaging contrast agent was developed by a facile fluorescent dye-coating on magnetic nanoparticles (MNP). The fluorescent dye molecules were directly attached onto the nanoparticle surface, which enable the nanoparticles to transfer from hydrophobic phase to hydrophilic phase. This facile synthesis method was applied to various fluorescent dyes and another hydrophobic nanoparticle to evaluate the synthetic principle. And the dye coating on the particle was well characterized by single particle imaging technique. We also demonstrated that photostability of fluorescent dyes on the particle surface were improved compared to the soluble dye form by monitoring of fluorescence signals under the continuous irradiation.

The dye-coated MNP presents high T2 relaxivity value (308 mM-1s-1), good cell viability due to biocompatible NIR dye, indocyanine green, supporting non-invasive in vivo MR/NIR imaging. We performed in vivo tracking of the dye-coated MNP labeled dendritic cells (DCs). Importantly, the migration of DCs via lymphatic drainage and homing into the lymph node were monitored through real-time NIR fluorescence and MR imaging. This novel MR/NIR contrast agent has potential applications as a non-invasive imaging agent for imaging or tracking of immune cells to confirm immunotherapeutic efficacy.

<figure><img src="image_14.png" /><figcaption>Abstract figure.jpg</figcaption></figure>
# 3D Printing Revolution for Nanotechnology and Nanomedicine

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 382

Dr. Alessandro Paolini¹, Ms. Antonella Celluzzi¹, Dr. Antonella Baldassarre¹, Dr. Simona Sennato², Dr. Francesco Mura³, Dr. Federico Bordi², Dr. Alberto Eugenio Tozzi¹, Dr. Andrea Masotti¹

1. Ospedale Pediatrico Bambino Gesù, 2. CNR-ISC UOS Roma, c/o Dipartimento di Fisica, Sapienza Università di Roma, 3. CNIS, Università di Roma Sapienza

## Introduction

The 3D printing is about to really transform our lives. While traditional laser and inkjet printers only make marks on paper, 3D printers build up solid objects one very thin layer over another. We are beginning to be surrounded by many prototypes, jewelry, sunglasses, works of art, toys and vehicle parts, but the next expected revolution is going to impact on the nanotechnology and biomedical fields.

## Methods

Here we show how the 3D prototyping of a cell culture device gives the opportunity to easily culture cells on a sheet of carbon nanotubes, referred as "buckypaper", and that these cells can be easily transduced with microRNAs, small RNA molecules able to regulate gene expression post-transcriptionally.

We printed 3D scaffolds using a commercial home-made printer (3DRag) and we studied polymer-coated buckypapers (BP) supported by them. We also assessed their toxicity compared to uncoated BP on Hek cells.

## Results

3D scaffolds enable to obtain a versatile support to study BP toxicity and cell culture adhesion and proliferation.

Polymer coated BPs have been demonstrated to be less toxic than the uncoated BP.

## Discussion

Interestingly, buckypaper is only one of the numerous examples of biomaterials that it is possible to employ in biomedical applications for culturing cells. Therefore, we envisage that in the near future a growing number of materials and potential applications in the biomedical field will appear on the horizon. 3D printed devices can open new perspectives not only for targeted manufacturing in the biomedical field but also for producing novel materials and 'bio-devices' for nanomedicine applications.
# Synthesis of High Sensitive Magnetic Nanoparticles and Application for Inflammation Detection

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 399

Mr. Jongeun Kang¹, Mr. Hyunseung Lee¹, Dr. Kwan Soo Hong¹

1. Bioimaging Research Team, Korea Basic Science Institute

Magnetic nanoparticles (MNPs) have been widely used as contrast agents for magnetic resonance imaging (MRI), drug delivery, and hyperthermia agents. Among them, MRI is one of the important applications of MNPs because the low sensitivity of MRI can be improved by MNPs. The improvement of T2 relaxivity of MNPs still remains as a critical issue. In this study, we reported an easy and large scale-up synthesis technique for high sensitive MNPs via pH regulation during co-precipitation-based method. A large amount of the aggregated MNPs by regulating pH were successfully coated with silica, in which the number of MNP cores in the silica was simply controlled by pH. As a result, the MNPs could form a nanocluster by decreasing repulsion force for each other when the particle surface charge is almost zero in neutral pH state. We report two types of silica-coated magnetic nanoclusters (SMCs) synthesized at pH2.0 and pH4.5, named as SMC2.0 and SMC4.5, respectively. SMC4.5 provides improved T2 relaxivity (270 mM-1s-1 vs. 155 mM-1s-1 for SMC2.0) due to increased number of cores (18 and 6 for SMC4.5 and SMC2.0, respectively). For in vivo T2*-weighted MRI experiments of inflammation animal model, SMC4.5 provided more specific and high sensitive detection of inflammation following in vivo labeling of the SMC4.5 in macrophages in the sites, showing a possibility of minimum dosage due to higher sensitivity. The nanocluster-type SMCs could be a T2 MRI contrast agent with minimum side-effect disease diagnosis and bio-imaging applications.

<figure><img src="image_15.png" /><figcaption>.png</figcaption></figure>
# Use of cell membrane permeating peptides for delivery of plasmid DNA into dendritic cells

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 415

Ms. Dominika Hobernik¹, Dr. A. James Mason², Prof. Stephan Grabbe¹, Dr. Matthias Bros¹

1. Department of Dermatology, University Medical Center of the Johannes Gutenberg University Mainz, 2. Institute of Pharmaceutical Science, King's College London

Immune therapy is a promising approach in the treatment of cancer. Besides ex vivo incubation of dendritic cells (DC) with tumor-associated antigens (TAA) and adjuvants, an increasing number of nanosized systems that co-deliver these compounds are assessed for effective in vivo drug delivery. DNA vaccines that encode for TAAs are an inexpensive, stable and safe alternative to protein- and peptide-based vaccines. Still, efficient delivery of pDNA in vivo is a challenge that needs to be faced.

LAH4-L1 is a cell membrane permeating peptide shown to complex pDNA and RNA. We analysed the suitability of this peptide to deliver pDNA to DC. First, we incubated murine spleen cells with fluorescently labelled LAH4-L1/pDNA complexes and analysed transfer of fluorescently labelled pDNA to different immune cell subtypes via flow cytometry. Due to strong interaction with endocytically active macrophages and DC, we also analysed bone marrow-derived dendritic cells (BMDC). BMDC showed strong binding/internalization of LAH4-L1 complexed pDNA. Unexpectedly, these BMDC also showed strong upregulation of surface markers that indicate cellular activation (MHCII, CD40, CD80, and CD86). In agreement, LAH4-L1/pDNA-treated BMDC generated strongly enhanced levels of proinflammatory cytokines (IL-1β, TNF-a, IL-6). These findings suggest that LAH4-L1 may exert a potent stimulatory capacity on DC which is crucial for the induction of an adaptive immune response. LAH4-L1 mediated stronger transfection efficiency than JetPEI, often used for the transfection of DC-like cell lines with transgene-encoding pDNA, as assessed using a luciferase reporter. Ongoing work is focussed on the use of LAH4-L1 for transfection of DC populations with antigen-encoding mRNA and pDNA and subsequent DC/T cell co-cultures to assess their T cell stimulatory capacity.

Future work is focussed on functionalization of LAH4-L1 with targeting moieties to target DC specifically. Furthermore, TAA-encoding pDNA will be used for in vivo proliferation assays and to induce tumor-specific immune responses.
# Efficient gene silencing with siRNA formulated in DOP-DETA-based lipid nanoparticles

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 420

Dr. Tomohiro Asai¹, Ms. Mariko Sako¹, Ms. Jiao Li¹, Ms. Ayaka Okamoto¹, Dr. Takehisa Dewa², Prof. Naoto Oku¹

1. University of Shizuoka School of Pharmaceutical Sciences, 2. Nagoya Institute of Technology

Development of small interfering RNA (siRNA) therapeutics is a promising approach to address unmet medical needs. However, because RNA is easily degraded by RNases and hardly internalized into the cells, a suitable delivery system is required to develop siRNA therapeutics. Development of a siRNA delivery vehicle is the most urgent and crucial thing for clinical success of siRNA-based therapies. In the present study, we designed and synthesized a novel polycationic lipid derivative, a dioleoylphosphate-diethylenetriamine conjugate (DOP-DETA) for the purpose of efficient siRNA delivery. Polycationic liposomes composed of DOP-DETA, cholesterol, and dipalmitoylphosphatidylcholine (DPPC) were prepared and mixed with siRNA to obtain lipid nanoparticles (LNP) loaded with siRNA. To evaluate the delivery potential of the LNP, a reporter gene silencing assay was performed using human fibrosarcoma cell line HT1080 constitutively expressing green fluorescent protein (GFP). The data showed that the LNP loaded with siRNA for GFP induced gene silencing at the concentration of less than 5 nM of siRNA. The gene silencing efficiency and cellular uptake amount of siRNA were dependent on siRNA/lipid molar ratio and increased with increasing concentration of lipids. Confocal microscopic observation demonstrates that siRNA transfected with the LNP diffusely distributed throughout the cytoplasm of the cells. Next, the LNP loaded with siRNA for poly [ADP-ribose] polymerase 1 (PARP1-siRNA) was prepared to evaluate its potential to suppress the expression of the innate protein. The results showed that knockdown of PARP1 protein was markedly observed when murine Lewis lung carcinoma cells had been transfected with 0.5 nM of PARP1-siRNA. The silencing efficiency of the LNP loaded with PARP1-siRNA was approximately 80-fold higher than that obtained with the common transfection reagents. The present findings suggest that DOP-DETA-based LNP might have excellent potential to deliver siRNA to target cells.
# Glimepiride solid lipid nanoparticles: Formulation, Evaluation and In-Vivo study.

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 430

Dr. omneya khowessah¹

1. Faculty of pharmacy, Cairo university

**Introduction:** Solid lipid nanoparticles(SLNs) have been reported as an alternative drug delivery system to traditional polymeric nanoparticles. Many studies were carried out and aimed to determine the enhancement ability of (SLNs) on the solubility of many poorly water soluble drugs and consequently, the improvement of their bioavailability.

**Methodolgy:** To enhance the bioavailability of glimepiride, SLNs were formulated using two different polymers (compritol and glycelymonostearate) in a fixed drug: lipid polymer ratio (1:20). Two different ratios of three different surfactants were used. GMP-SLNs were prepared adapting two different techniques namely, hot fusion method and solvent emulsification method. The prepared GMP-SLNs were evaluated with respect to encapsulation efficiency, yield value, particle size, zeta potential, image analyzer, and TEM analyzer. The DSC analysis and PXRD analysis were carried out on selected formulae. The pharmacokinetic study of the selected GMP-SLNs formula (F2) was carried out on male Newzeland white rabbits and the results were compared with those of marketed product (Amaryl® 4mg tablets).

**Results:** The resulted values revealed that most of the solid lipid nanoparticles were of circular shape, the particle size ranging from 104 nm to 334 nm with zeta potential ranged from -9.34 mV to -38.6 mV. The resulted EE% values ranged from 97.8±0.25% to 99.7±0.23%. The yield values were from 97.88±2.37% to 98.96±2.03%. TEM analysis of SLNs formulae F2 and F8 showed rounded and discrete shaped particles. The DSC analysis results of F2 and F8 showed that there were no interactions between the drug and the used excipients. PXRD diffracgrams of F2 and F8 showed lamellar lattice in case of SLNs and crystalline lattice in case of the compritol, glyceryl monostearate and drug powders in bulk state. The pharmacokinetic study and statistical analysis of the selected formulae (F2) showed that (F2) significantly enhanced the rate of GMP absorption from GIT and at 0.5, 2 & 6 hrs compared to the marketed product. The resulted Cmax was 28.1mg/ml, Tmax was 5.94 hrs, t1/2 abs was 1.53 hrs, t1/2ele. was 18.1 hrs, (maximum amount of drug absorbed) was 2018 µg, F was 1 and the bioavailability (F relative) was 100%.
<table><caption>Table.png</caption><thead><tr><th></th><th>(%±S.D)</th><th>Size<br>(nm±<br>S.D)</th><th>(mV±<br>S.D)</th><th>(%Yield±<br>S.D)</th></tr></thead><tbody><tr><td rowspan="2">F1</td><td>98.8±</td><td>352.4</td><td>0.453</td><td>-33.2</td><td>98.88±1.62</td></tr><tr><td>0.30</td><td>±22</td><td>±0.061</td><td>±0.05</td><td></td></tr><tr><td rowspan="2">F2</td><td>99.4±</td><td>246.6</td><td>0.324</td><td>-37.5</td><td>99.19±1.54</td></tr><tr><td>0.66</td><td>±30</td><td>±0.038</td><td>±0.07</td><td></td></tr><tr><td rowspan="2">F3</td><td>99.7±</td><td>465.8</td><td>0.658</td><td>-24.3</td><td>97.88±2.37</td></tr><tr><td>0.23</td><td>±17</td><td>±0.023</td><td>±0.03</td><td></td></tr><tr><td rowspan="2">F4</td><td>99.3±</td><td>342.6</td><td>0.531</td><td>-28.8</td><td>98.96±2.03</td></tr><tr><td>0.12</td><td>±23</td><td>±0.072</td><td>±0.04</td><td></td></tr><tr><td rowspan="2">F5</td><td>97.8±</td><td>875.4</td><td>0.757</td><td>-13.2</td><td>97.91±1.63</td></tr><tr><td>0.25</td><td>±42</td><td>±0.033</td><td>±0.06</td><td></td></tr><tr><td rowspan="2">F6</td><td>98.7±</td><td>552.3</td><td>0.302</td><td>-11.5</td><td>98.24±3.23</td></tr><tr><td>0.42</td><td>±27</td><td>±0.026</td><td>0.05</td><td></td></tr><tr><td rowspan="2">F7</td><td>99.5±</td><td>843.6</td><td>0.637</td><td>-27.4</td><td>98.74±1.07</td></tr><tr><td>0.32</td><td>±38</td><td>±0.044</td><td>±0.06</td><td></td></tr><tr><td rowspan="2">F8</td><td>99.3±</td><td>622.4</td><td>0.462</td><td>-34.3</td><td>99.15±1.06</td></tr><tr><td>0.81</td><td>±26</td><td>±0.037</td><td>±0.07</td><td></td></tr><tr><td rowspan="2">F9</td><td>99.6±</td><td>644.2</td><td>0.476</td><td>-22.2</td><td>98.87±3.08</td></tr><tr><td>0.31</td><td>±34</td><td>±0.072</td><td>±0.08</td><td></td></tr><tr><td rowspan="2">F10</td><td>99.3±</td><td>487.4</td><td>0.323</td><td>-25.1</td><td>98.31±1.82</td></tr><tr><td>0.70</td><td>±27</td><td>±0.025</td><td>±0.09</td><td></td></tr><tr><td rowspan="2">F11</td><td>99.7±</td><td>784.4</td><td>0.622</td><td>-12.4</td><td>98.68±2.08</td></tr><tr><td>0.21</td><td>±38</td><td>±0.044</td><td>±0.06</td><td></td></tr><tr><td rowspan="2">F12</td><td>99.5±</td><td>579.0</td><td>0.412</td><td>-10.2</td><td>98.32±2.05</td></tr><tr><td>0.53</td><td>±28</td><td>±0.062</td><td>±0.08</td><td></td></tr></tbody></table>
# A novel immunosensor based on polypyrrole electroless deposition on silicon nitride substrates: Interleukine-10 detection

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 478

Ms. Faiza Nessark¹, Dr. Abdoullatif Baraket², Dr. Nadia Zine³, Prof. Ahmed Zouaoui⁴, Prof. Belkacem Nessark⁵, Prof. Joan Bausells⁶, Prof. abdelhamid Errachid³

1. Laboratoire d'Electrochimie et Matériaux (LEM), Université Ferhat Abbas Sétif 1, 19000, Algérie, 2. Université Claude Bernard Lyon1, 3. Institut des sciences Analytiques (ISA), Université Claude Bernard Lyon1, UMR 5280, 5 rue de la Doua, 69100 Villeurbanne Lyon, 6 France, 4. Laboratoire de Croissance et Caractérisation de Nouveaux Semi-conducteurs (LCCNS), Université Ferhat Abbas Sétif 1, 19000, Algeria, 5. Laboratoire d'Electrochimie et Matériaux (LEM), Université Ferhat Abbas Sétif 1, 19000, Algeria, 6. Instituto de Microelectronica de Barcelona, IMB-CNM (CSIC), Campus UAB, 08193, Bellaterra, Spain.

We report in present study about development of a novel strategy of anti-human interleukin-10 (anti-IL-10) antibody immobilization onto polypyrrole (PPy) modified capacitance substrate. Here, we propose the development of a biosensor based on silicon nitride (Si3N4) chemically modified by a deposition of a PPy layer. In order to ensure adhesion of the conductive organic polymer to the substrate, a silane-pyrrole reagent (SPy) N-(3-trimethoxysilylpropyl) pyrrole was used to create a covalent bridge between the silicon substrate and the layer of PPy (Scheme 1). The surface morphology of the composite Si3N4-P(SPy-Py) was studied by scanning electron microscopy (SEM) and infrared spectroscopy (FTIR). The electrochemical behavior of the novel composite Si3N4-P(SPy-Py) was characterized by cyclic voltammetry (CV) and electrical impedance spectroscopy (EIS). The (Si3N4)-P(SPy-Py) composite was subsequently electrochemically modified by diazonium salts (4-Aminophenylacetic acid) (CMA) for the immobilization of specific antibodies (anti-human interleukin-10 monoclonal antibody) by carbodiimide chemistry. The configured immunosensor successfully detected human IL-10 in the range of 1-50 pg/mL. This new composite shows a high sensitivity and a novel approach for the development of new strategies for biosensor development and detection.

<figure><img src="image_16.png" /><figcaption>Scheme 1. Schematic representation of: (a) activated Si3N4, (b) Si3N4-SPy, and (c) the development of Si3N4-P(SPy-Py) compound materials.</figcaption></figure>
# Biophysical Characterization, Nanoscale Composition and Cell Uptake Studies of pH-Sensitive Drug Delivery Systems

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 490

Dr. Jana B. Nieder¹, Ms. Rasa Ozolina², Ms. Ana M. Carvalho³, Ms. Vânia Vilas-Boas², Prof. M.E.C.D. Real Oliveira³, Dr. Marlene Lúcio³

1. INL-International Iberian Nanotechnology Laboratory, 2. INL - International Iberian Nanotechnology Laboratory, 3. University of Minho

Nanocarrier-based chemotherapy is one of the few nanotechnology-based medical therapies that reached the clinics. This happened already in 1995, when the commercial liposomal anti cancer drug delivery formulation DOXIL® was introduced in the market. Albeit these early developments, still today nanotechnology-based drug delivery systems are far from reaching optimal selectivity and controlled release ability.

In our study we use different liposomal formulations designed for pH-sensitive drug release and study their biophysical characteristics, when used for trafficking paclitaxel (PTX) and doxorubicin (DOX), both widely used chemotherapeutic anti-cancer drugs.

Our work describes a combined spectroscopy and imaging approach to evaluate the biophysical properties of liposomal formulations. We study the nanoscale composition of the nanocarriers using molecular rulers in a fluorescence quenching assay, and analyze the cell uptake characteristics based on the autofluorescence of DOX using confocal microscopy.

<figure><img src="image_17.png" /></figure>

Figure: Cell uptake study: Confocal images taken on fixed cells incubated with liposomal formulations and free DOX.

Nieder-celluptakeiconan2016.png
Effect of Nanosilver with salicylic acid & nano salicylic acid pre-treatment on Antioxidant responses in Dracocephalum moldavica L.

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 513

Prof. Hossein Abbaspour ¹, Dr. zahra haghighipak ¹, Dr. Naser Karimi ²

1. Damghan Branch, Islamic Azad University, 2. Razi University

Nanoparticles (NPs) with different composition, size, and concentration have been reported to influence growth and development of various plant species with both positive and negative effects. Ag-nanoparticle is important for its characteristic physiochemical and biological properties. Dracocephalum moldavica L. is well known as medicinal plants because of its biological and pharmacological properties. Salicylic acid (SA) is an important signal molecule modulating plant response to stress. In here, we investigated some antioxidative responses of D. moldavica under Ag-NPs pretreated with different SA and nano-SA concentrations. The study was carried out in a randomized block design with 3 replications in four levels of SA and nano SA (0, 20, 60, 80 mM) and six Ag-NPs treatments (0, 10, 20, 40, 60, 80 PPm) for 21 days. AgNPs significantly induced oxidative stress marker such as H2O2 content in a concentration dependent manner. The phytotoxicity of AgNPs led to an increase in catalase and peroxidase activities and synthesis of antioxidant compounds such as carotenoids, proline and total soluble carbohydrate. Further results showed that SA and nano-SA pretreatments ameliorates the Ag-phytotoxicity, which nano-SA was more effective than SA in this manner.
# Formulation of Levofloxacin Loaded Niosomal suspensions as an Ocular Delivery System: In-Vitro Evaluation and Microbiological Study.

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 391

Dr. salwa hussien¹

1. Faculty of pharmacy, Cairo university

## INTRODUCTION

Levofloxacin hemihydrate is a fluoroquinolone antibiotic with broad spectrum antibacterial agent. Encapsulation of the drug into vesicular system provides delivering drug in a controlled manner to enhance bioavailability and get therapeutic effect over a longer period of time. Niosomes are such hydrated vesicular systems containing nonionic surfactants along with cholesterol or other lipids delivering drug to targeted site which are non toxic, requiring less production cost, stable over a long period of time in different conditions.

## METHODS

Niosomes of levofloxacin hemihydrate were prepared by reverse evaporation method. Optimization of the formulation step was carried by application of 5x3x2 factorial design to study the influence of three parameters including surfactant type, cholesterol molar ratio and surfactant molar ratio on the physicochemical properties including particle size, entrapment efficiency (E.E) and release parameters (Q8h and Q24h). Transmission electron microscope (TEM) was carried out for the selected formula. Microbiological study of the selected formula was also investigated.

## RESULTS AND DISCUSSION

Niosomes prepared from span 40 and span60 showed the highest entrapment efficiency. The best formula was the one which prepared from span60 and cholesterol with molar ratio(7:6) of entrapment efficiency 84.1% and particle size (587nm). In-vitro drug release results confirmed that niosomal formulations have exhibited a high retention of levofloxacin inside the vesicles and can sustain release up to 24 hours. The in-vitro evaluation of levofloxacin hemihydrate niosomes in comparison to levofloxacin hemihydrate solution showed the effect of niosomes in prolongation of drug release from the ocular delivery system. Niosomes may be considered as promising ophthalmic carriers for the topical application of levofloxacin hemihydrates. The antimicrobial activity of the selected formula was retained up to 24 hours.
<table><caption>Niosomes table.png</caption><thead><tr><th>Niosomes Table</th><th>Target Nucleus</th><th>Target Nucleus</th></tr></thead><tbody><tr><td>1</td><td>Target 19</td><td>Target 19</td></tr><tr><td>2</td><td>Target 19</td><td>Target 19</td></tr><tr><td>3</td><td>Target 19</td><td>Target 19</td></tr><tr><td>4</td><td>Target 19</td><td>Target 19</td></tr><tr><td>5</td><td>Target 19</td><td>Target 19</td></tr><tr><td>6</td><td>Target 19</td><td>Target 19</td></tr><tr><td>7</td><td>Target 19</td><td>Target 19</td></tr><tr><td>8</td><td>Target 19</td><td>Target 19</td></tr><tr><td>9</td><td>Target 19</td><td>Target 19</td></tr><tr><td>10</td><td>Target 19</td><td>Target 19</td></tr><tr><td>11</td><td>Target 19</td><td>Target 19</td></tr><tr><td>12</td><td>Target 19</td><td>Target 19</td></tr><tr><td>13</td><td>Target 19</td><td>Target 19</td></tr><tr><td>14</td><td>Target 19</td><td>Target 19</td></tr><tr><td>15</td><td>Target 19</td><td>Target 19</td></tr><tr><td>16</td><td>Target 19</td><td>Target 19</td></tr><tr><td>17</td><td>Target 19</td><td>Target 19</td></tr><tr><td>18</td><td>Target 19</td><td>Target 19</td></tr><tr><td>19</td><td>Target 19</td><td>Target 19</td></tr><tr><td>20</td><td>Target 19</td><td>Target 19</td></tr><tr><td>21</td><td>Target 19</td><td>Target 19</td></tr><tr><td>22</td><td>Target 19</td><td>Target 19</td></tr><tr><td>23</td><td>Target 19</td><td>Target 19</td></tr><tr><td>24</td><td>Target 19</td><td>Target 19</td></tr><tr><td>25</td><td>Target 19</td><td>Target 19</td></tr><tr><td>26</td><td>Target 19</td><td>Target 19</td></tr><tr><td>27</td><td>Target 19</td><td>Target 19</td></tr><tr><td>28</td><td>Target 19</td><td>Target 19</td></tr><tr><td>29</td><td>Target 19</td><td>Target 19</td></tr><tr><td>30</td><td>Target 19</td><td>Target 19</td></tr><tr><td>31</td><td>Target 19</td><td>Target 19</td></tr><tr><td>32</td><td>Target 19</td><td>Target 19</td></tr><tr><td>33</td><td>Target 19</td><td>Target 19</td></tr><tr><td>34</td><td>Target 19</td><td>Target 19</td></tr><tr><td>35</td><td>Target 19</td><td>Target 19</td></tr><tr><td>36</td><td>Target 19</td><td>Target 19</td></tr><tr><td>37</td><td>Target 19</td><td>Target 19</td></tr><tr><td>38</td><td>Target 19</td><td>Target 19</td></tr><tr><td>39</td><td>Target 19</td><td>Target 19</td></tr><tr><td>40</td><td>Target 19</td><td>Target 19</td></tr><tr><td>41</td><td>Target 19</td><td>Target 19</td></tr><tr><td>42</td><td>Target 19</td><td>Target 19</td></tr><tr><td>43</td><td>Target 19</td><td>Target 19</td></tr><tr><td>44</td><td>Target 19</td><td>Target 19</td></tr><tr><td>45</td><td>Target 19</td><td>Target 19</td></tr><tr><td>46</td><td>Target 19</td><td>Target 19</td></tr><tr><td>47</td><td>Target 19</td><td>Target 19</td></tr><tr><td>48</td><td>Target 19</td><td>Target 19</td></tr><tr><td>49</td><td>Target 19</td><td>Target 19</td></tr><tr><td>50</td><td>Target 19</td><td>Target 19</td></tr><tr><td>51</td><td>Target 19</td><td>Target 19</td></tr><tr><td>52</td><td>Target 19</td><td>Target 19</td></tr><tr><td>53</td><td>Target 19</td><td>Target 19</td></tr><tr><td>54</td><td>Target 19</td><td>Target 19</td></tr><tr><td>55</td><td>Target 19</td><td>Target 19</td></tr><tr><td>56</td><td>Target 19</td><td>Target 19</td></tr><tr><td>57</td><td>Target 19</td><td>Target 19</td></tr><tr><td>58</td><td>Target 19</td><td>Target 19</td></tr><tr><td>59</td><td>Target 19</td><td>Target 19</td></tr><tr><td>60</td><td>Target 19</td><td>Target 19</td></tr><tr><td>61</td><td>Target 19</td><td>Target 19</td></tr><tr><td>62</td><td>Target 19</td><td>Target 19</td></tr><tr><td>63</td><td>Target 19</td><td>Target 19</td></tr><tr><td>64</td><td>Target 19</td><td>Target 19</td></tr><tr><td>65</td><td>Target 19</td><td>Target 19</td></tr><tr><td>66</td><td>Target 19</td><td>Target 19</td></tr><tr><td>67</td><td>Target 19</td><td>Target 19</td></tr><tr><td>68</td><td>Target 19</td><td>Target 19</td></tr><tr><td>69</td><td>Target 19</td><td>Target 19</td></tr><tr><td>70</td><td>Target 19</td><td>Target 19</td></tr><tr><td>71</td><td>Target 19</td><td>Target 19</td></tr><tr><td>72</td><td>Target 19</td><td>Target 19</td></tr><tr><td>73</td><td>Target 19</td><td>Target 19</td></tr><tr><td>74</td><td>Target 19</td><td>Target 19</td></tr><tr><td>75</td><td>Target 19</td><td>Target 19</td></tr><tr><td>76</td><td>Target 19</td><td>Target 19</td></tr><tr><td>77</td><td>Target 19</td><td>Target 19</td></tr><tr><td>78</td><td>Target 19</td><td>Target 19</td></tr><tr><td>79</td><td>Target 19</td><td>Target 19</td></tr><tr><td>80</td><td>Target 19</td><td>Target 19</td></tr><tr><td>81</td><td>Target 19</td><td>Target 19</td></tr><tr><td>82</td><td>Target 19</td><td>Target 19</td></tr><tr><td>83</td><td>Target 19</td><td>Target 19</td></tr><tr><td>84</td><td>Target 19</td><td>Target 19</td></tr><tr><td>85</td><td>Target 19</td><td>Target 19</td></tr><tr><td>86</td><td>Target 19</td><td>Target 19</td></tr><tr><td>87</td><td>Target 19</td><td>Target 19</td></tr><tr><td>88</td><td>Target 19</td><td>Target 19</td></tr><tr><td>89</td><td>Target 19</td><td>Target 19</td></tr><tr><td>90</td><td>Target 19</td><td>Target 19</td></tr><tr><td>91</td><td>Target 19</td><td>Target 19</td></tr><tr><td>92</td><td>Target 19</td><td>Target 19</td></tr><tr><td>93</td><td>Target 19</td><td>Target 19</td></tr><tr><td>94</td><td>Target 19</td><td>Target 19</td></tr><tr><td>95</td><td>Target 19</td><td>Target 19</td></tr><tr><td>96</td><td>Target 19</td><td>Target 19</td></tr><tr><td>97</td><td>Target 19</td><td>Target 19</td></tr><tr><td>98</td><td>Target 19</td><td>Target 19</td></tr><tr><td>99</td><td>Target 19</td><td>Target 19</td></tr><tr><td>100</td><td>Target 19</td><td>Target 19</td></tr></tbody></table>
# Sickle cell hemoglobin detection in drying drops: from protein/nanoparticle interaction to low resource diagnostic tools

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 459

Dr. Stephanie Devineau¹, Dr. Manos Anyfantakis², Mr. Laurent Marichal³, Dr. Laurent Kiger⁴, Prof. Damien Baigl²

1. Centre For BioNano Interactions (CBNI), University College Dublin, 2. Department of Chemistry, Ecole Normale Superieure,
3. CEA Saclay, NIMBE, 4. Inserm, IMRB

The coffee-ring effect denotes the accumulation of particles at the edge of an evaporating drop pinned on a substrate, as one can observe in the black ring of a coffee stain. Because it can be detected by simple visual inspection, this ubiquitous phenomenon has been recently combined with specific particle formulations to develop robust and cost-effective diagnostic tools. Here we analysed the deposit morphology of drying drops containing polystyrene nanoparticles of different surface properties with human native and mutant hemoglobin. We show that deposit patterns reveal information on both the adsorption of proteins on the particles and their reorganization following adsorption. We then established the conditions that enable to distinguish between native and sickle cell hemoglobin by simple pattern analysis. The suppression of the coffee ring effect and the formation of a disk-shaped pattern is primarily associated to particle neutralization by protein adsorption. However, our findings also suggest that protein reorganization following adsorption can dramatically invert this tendency. Exposure of hydrophobic residues can lead to disk deposit morphologies independently of the global particle charge due to accumulation of particles at the liquid/gas interface during evaporation. This general behaviour opens the possibility to probe protein adsorption and reorganization on particles by the analysis of the deposit patterns, the formation of a disk being the robust signature of particles rendered hydrophobic by protein adsorption. This method is sensitive enough to detect a single point mutation in a protein, as demonstrated by the distinct patterns formed by human native hemoglobin HbA and its mutant form HbS responsible for sickle cell anemia (1). The coffee-ring effect can translate various types of molecular interactions between nanoparticles and proteins into easily distinguishable macroscopic patterns, thus providing valuable insights for the development of future cost-effective diagnostic tools.

## Reference

(1) Devineau S, Anyfantakis M, Marichal L, Kiger L, Morel M, Rudiuk S, Baigl D. Protein adsorption and reorganization on nanoparticles probed by the coffee-ring effect: application to single point mutation detection. JACS (2016) 138, 11623-11632
Sickle cell hemoglobin detection in drying drops

<figure><img src="image_18.png" /></figure>

<figure><img src="image_19.png" /></figure>

<figure><img src="image_20.png" /></figure>

Native
hemoglobin

Sickle cell
hemoglobin

Sickle cell hemoglobin detection in drying drops.jpg
Molecular mechanism and increased antileishmanial
activity of carbon nanotube based betulin formulation

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 473

Dr. Prakash Saudagar ¹

1. National Institute of Technology Warangal

The present study describes a novel antileishmanial drug formulation of betulin (BET) attached to functional-
ized carbon nanotubes (f-CNTs) as macrophage targeted drug delivery system. We conjugated BET; a pentacyclic
triterpenoid secondary metabolite to carboxylic acid chains on f-CNTs to obtain BET attached functionalized
carbon nanotubes (f-CNT-Bet). The fourier transform infrared (FTIR) spectroscopy and transmission electron
microscopy (TEM) demonstrated the successful construction of f-CNT-BET. The Bet loading efficiency onto the
f-CNTs and Bet release profile were monitored spectroscopically. The drug release profile demonstrated a fairly
slow release of Bet. The in-vitro cytotoxicity of BET, f-CNT and f-CNT-BET on J774A.1 macrophage cell line were
211.05 ± 7.14 µg/ml; 24.67 ± 3.11 µg/ml and 72.63 ± 6.14 µg/ml respectively as measured by the cytotoxic con-
centration required to inhibit 50% cell viability (IC50). The IC50 of BET and f-CNT-BET against intracellular
L. donovani amastigote in vitro were 8.33 ± 0.41 µg/ml and 0.69 ± 0.08 µg/ml respectively. The results clearly
demonstrating the greater antileishmanial efficiency of f-CNT-BET formulation than BET alone and with no
significant cytotoxicity observed on host cells, f-CNT-BET boost new hope for significant application of carbon
nanotubes in formulating better drug for leishmaniasis with minimum side effects and high treatment efficacy.

<figure><img src="image_21.png" /><figcaption>Antileishmanial activity of f-cnt-bet.jpg</figcaption></figure>
# At focus compressed 7 and 70 femtosecond pulses for deep tissue multiphoton microscopy

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 489

Dr. Christian Maibohm¹, Dr. Francisco Silva², Dr. Edite Figueiras¹, Dr. Paulo T. Guerreiro², Dr.

Marina Brito¹, Dr. Rosa Romero², Dr. Helder Crespo³, Dr. Jana B. Nieder¹

1. INL-International Iberian Nanotechnology Laboratory, 2. Sphere Ultrafast Photonics, 3. University of Porto

We compare the contrast, signal intensities and excitation selectivity of a standard Ti:Sa Tsunami oscillator laser typically used in multi-photon microscopy with about 150 fs pulse duration and compare the performance with an ultrashort laser source with 7 fs short pulses. Pulse compression and measurement was performed at the focus of the objective with a new microscope compatible with the d-scan laser metrology based transmission head.

Using such pulse compression technique we were able to reduce the Tsunami pulse length at the focus of the objective that mainly is affected by the dispersion of the high NA (1.4) microscope objective itself and obtained nominal Fourier Transform limited pulses with 70 fs pulse duration at the position of the sample.

We use a multicolor-labeled 2D FluoCells® (Thermofisher) test sample as well as a pig artery tissue sample to compare contrast, intensity and in addition the deep tissue imaging performance and can report substantial improvements of these parameters using at-focus compressed femtosecond laser pulses. These improvements could play a major role for rendering multiphoton microscopy an excellent tool for noninvasive optical investigation of bioengineered tissues and to drug delivery studies inside tissues.

<figure><img src="image_22.png" /></figure>

Figure: Multiphoton image using an ultrashort femtosecond laser source with 7fs pulse duration for a horizontal cut of a pig artery

Abstract picture.jpg 2.jpg
# In vivo anti-inflammatory activity by trans-resveratrol loaded-solid lipid nanoparticle for skin disorders.

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 553

Ms. Roberta Rigon¹, Ms. Maíra Gonçalez², Ms. Camila Rodero², Dr. Marlus Chorilli²

1. Universidade Estadual Paulista "Júlio de Mesquita Filho", Araraquara, Brazil, 2. Universidade Estadual Paulista "Júlio de Mesquita Filho", Araraquara, Brazil

Trans-resveratrol (RES) presents important action to prevent and treat skin disorders. Cutaneous administra-
tion of RES has being explored in order to find it on their site of action [1]. Solid lipid nanoparticles (SLN)
due to interaction characteristics with skin have being used to drug delivery system for topical application [2].
The aim was verify in vivo anti-inflammatory activity of SLN with RES for cutaneous application therapy. The
formulations developed were composed by stearic acid (SA) as solid lipid, polysorbate 80 (P80) and soy phos-
phatidylcholine (SP) as surfactants, and poloxamer 407 (P407) and glycerin as stabilizers (F1). Cetrimonium
bromide (CB) was added as cationic surfactant to promote positive superficial charge (F2). The formulations
was add of 0.25% RES (F1.RES and F2.RES). The anti-inflammatory and antinociceptive activities of SLNs were
measured by carrageenan-induced paw edema and paw pressure test in mice, respectively [3,4]. This research
was approved by Ethics Research Committee of UNESP (nº 68/2015). The average hydrodynamic diameters were
195.0 ± 3.34 nm, 241.3 ± 48.33 nm, 159.15 ± 4.78 nm e 158.25 ± 33.92 nm to F1, F1.RES, F2 e F2.RES, respectively.
Zeta potentials (mV) were -25.5 ± 1.01; -26.0 ± 1.67; 30.6 ± 1.13 e 30.0 ± 1.85 mV for F1, F1.RES, F2 e F2.RES,
respectively. Entrapment efficacy was analyzed using validated analytical methodology and both formulations
(F1.RES and F2.RES) present 50% of RES entrapped. RES solution (1:1 ethanol and water) and F2.RES presented
reduction of nociception similar to dexametasone commercial cream, which suggest potent anti-inflammatory
activity of RES and F2.RES. Stratum corneum have a negative surface charge that permits greater bioadhesion
of positive particles surface, which could explain the increase of antinociceptive activity by F2.RES compared
to F1.RES. The results demonstrated the importance to investigate the RES action when it is entrapped in drug
delivery system for topical application.

References

[1] Ndiaye, Mary, et al. Archives of biochemistry and biophysics 508.2 (2011): 164-170.

[2] Wissing, Sylvia A., and Rainer H. Müller. International Journal of Pharmaceutics 254.1 (2003): 65-68.

[3] RANDALL, L. O. Arch Int Pharmacodyn, 111 (1957): 409-419.

[4] SANTOS-NOGUEIRA, E. et al. Journal of neurotrauma 29.5 (2012):898-904.
# Porous metal-organic-framework MIL-100(Fe) as a nanoscale platform for sustainable release of tetracycline

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 508

Mr. Seyed Dariush Taherzade ¹, Dr. Aliakbar Tarlani ², Dr. Janet Soleimannejad ¹

1. university of tehran, 2. Chemistry & Chemical Engineering Research Center of Iran

Tetracyclines are a group of broad-spectrum antibiotics used in the treatment of infections of urinary and respiratory tract [1]. Nanoscale porous MIL-100 is a great platform for delivery of broad types of drugs like doxorubicin, ibuprofen and etc [2, 3]. In current research nano MIL-100 was synthesized via Horcajada, et al. method [2]. Sample characterizations carried out with FT-IR, XRD, BET and SEM. Tetracycline (TC) was loaded on MIL-100 in the ratio of 1:3 and the nano-drug-carrier abbreviated as TC@Mil-100. FT-IR spectra confirmed the loading of the drug into the carrier framework. XRD pattern of TC@MIL-100 indicated no pattern of TC. On the other hand, BET analysis showed a 66 percent decrease in available pores and the free surface decreased to 801 Cm3g⁻¹, so it can be concluded that the drug is well dispersed into the nano-pores of MIL-100. The release of TC from MOF was investigated in Simulated Gastric Fluid (SGF) media during 48 hours via UV analysis and it was observed that 96 percent of the drug was sustain released which is an unprecedented amount in comparison with other methods[4]. The results demonstrated the substantially enhanced release of tetracycline as a very slightly soluble drug.

1. Chopra, I. and M. Roberts, Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiology and molecular biology reviews, 2001. 65(2): p. 232-260.

2. Horcajada, P., et al., Porous metal-organic-framework nanoscale carriers as a potential platform for drug delivery and imaging. Nature materials, 2010. 9(2): p. 172-178.

3. Agostoni, V., et al., Impact of phosphorylation on the encapsulation of nucleoside analogues within porous iron (III) metal-organic framework MIL-100 (Fe) nanoparticles. Journal of Materials Chemistry B, 2013. 1(34): p. 4231-4242.

4. Xie, J.F., et al. In Vitro Release Characterization of Tetracycline Hydrochloride from PLGA/Tetracycline Hydrochloride Electrospun Nanofiber Mats. in Advanced Materials Research. 2013. Trans Tech Publ.
Sustainable Release of TC in 48 Hours

<figure><img src="image_23.png" /><figcaption>Sustainable release of tc in 48 hours.png</figcaption></figure>

BRUKER

<figure><img src="image_24.png" /></figure>

BRUKER

<figure><img src="image_25.png" /><figcaption>Ft-ir comparison.png</figcaption></figure>

XRD Pattern

<figure><img src="image_26.png" /><figcaption>Xrd comparison.png</figcaption></figure>
# Characterisation of polyurethane nanocomposite hydrogel systems for wound dressing application.

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 552

**Ms. Marta Miotke**¹, Dr. Justyna Strankowska¹, Prof. Marek Józefowicz¹, Dr. Michał Strankowski², Prof. Jerzy Kwela¹

1. University of Gdańsk, 2. Gdańsk University of Technology

In recent years significant efforts have been made by many research groups for designing a new type of wound dressing. Large number of studies concern application of nontoxic, biodegradable polyurethanes which, moreover, are easy accessible and have wide possibilities to change their properties with modification. The goal which we would like to achieve is to obtain wound dressing with capabilities to faster wound healing and alleviation of pain by release of drug by delivery diffusion process. In order to obtain these properties, the process of increase of swelling ability of polymer matrix is important to achieve, for example, by adding nanoparticles which extend the intermolecular spaces in polyurethane matrix. The main purpose of our research is to achieve material with predetermined and well defined hardness, elasticity, and with appropriate swelling and release profiles. In previous papers we described method of synthesis and studies of basic mechanical properties (using DMA technique) and we examined structure of polyurethane nanocomposite by means of X-ray diffraction. The naproxen sodium – commonly used inflammatory drug, was chosen as an active substance potentially released from wound dressing. Before molecule reaches the target receptor, it changes the environment from polar protic – body fluids, to nonpolar cavity receptor. Understanding possible interaction between drug and solvent molecules in the ground and/or excited states was one of the main aim of this work. To achieve this goal, steady-state absorption and fluorescence spectra of naproxen in solvents of different polarities were registered. In addition, using steady-state spectroscopy methods, positive influence of presence of nanoparticles (organofilized montmorillonite – Cloisite®30B) in polymer matrix was observed. Analysis of release active substance from polyurethane matrix were determined basing on light absorption spectra. The concentration of naproxen was registered as a function of time. The most common mechanism of drug release from hydrogels is passive diffusion. In our case this process is guaranteed by presence of nanoparticles. Obtained results confirmed the high possibilities of polyurethane nanocomposite hydrogels as a new type of wound dressing material with the ability to release of drugs.
<figure><img src="image_27.png" /><figcaption>Graphical abstract.png</figcaption></figure>
# Curcumin-loaded cationic solid lipid nanoparticles as a potential platform for the treatment of melanoma

Wednesday, 28th September - 13:30 - Poster Session & Sponsors Exhibition - Patio 25 - Poster presentation - Abstract ID: 550

Ms. Maíra Gonçalez¹, Ms. Roberta Rigon¹, Dr. MarluS Chorilli¹

1. Universidade Estadual Paulista "Júlio de Mesquita Filho", Araraquara, Brazil

## INTRODUCTION

Curcumin (CUM) presents antineoplastic properties and its incorporation into cationic solid lipid nanoparticles (CSLN) is convenient in order to compartmentalize and delivery this drug to melanoma cells, can be used in melanoma prevention and treatment (1).

## METHODS

### Formulations preparation

All formulations were produced with Poloxamer 407 (3.2%), cetrimonium bromide (0.7%), water (91.1%), and solid lipids (5%), which were varied obtained F1 composed by stearic acid, F3 composed by Compritol 888 ATO and F2 composed by a blend of both lipids. The ingredients were mixtures and sonicated for 20 minutes at 12-17 W. It were performed determinations of mean hydrodynamic diameter (Dnm), polydispersity index (PdI) and zeta potential (ZP) of each formulation.

### Cell viability assay of HaCaT and B16-F10 cells by MTT technique

Healthy keratinocytes cells (HaCaT) and tumorigenic melanocytic cells (B16-F10) were treated with formulations, CUM-free, Doxorubicin solution (PC) and culture medium (NC). After the treatments, the cell viability was evaluated using the MTT technique.

## RESULTS AND DISCUSSION

There was obtained systems with Dnm of 218-238 nm, PdI of 0,185-0,350 and ZP of 25,6-30,1 mV.

Viability cells tests showed that the systems developed are more toxic to B16-F10 than HaCat and the incorporation of CUM in CSLNs increase the toxicity of this drug to melanoma cells (B16F10), because the positive charge presented by these systems may promote drug targeting to melanoma cells, that have a high density of negative charge due to high exposure of phosphatidylserine on the surface of cell (2), showing that the CUM-loaded-CSLN are promising for the treatment of melanoma (Figure 1).

(Attach figure 1)

Figure 1. Cell viability assay of HaCaT (a) and B16F10 (b), after exposed to treatments.

## REFERENCES

1. Sharma R.A. et al. Eur J Cancer, 41, 1955-1968, 2005.

2. Sakamoto, J. H. et al., Pharmacol. Res., 62, 57-89, 2010.

## ACKNOWLEDGEMENTS

This work was financially supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP).
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Peptide functionalised nanoparticles for the selective
induction of apoptosis in target cells

Wednesday, 28th September - 16:00 - Targeted drug delivery and Nanocarriers - Nanomedecine for cancer diagnosis & therapy - Amphitheatre 25 - Oral presentation - Abstract ID: 434

Prof. Mervin Meyer ¹, Dr. Nicole Sibuyi ¹, Dr. Ntevheleni Thovhogi ¹, Prof. Martin Onani ¹, Dr. Amanda Skepu ², Dr. Abram Madiehe ³

1. University of the Western Cape, 2. Mintek, 3. Council for Scientific and Industrial Research

Anti-angiogenic therapies are practical strategies for the treatment of both obesity and cancer. The aim of this study was to develop gold nanoparticles (AuNPs) for targeted anti-angiogenic therapy. The objective was to develop AuNPs that can selectively deliver pro-apoptotic peptides to the endothelial cells of the vascular networks that supply the white adipose tissue (WAT), causing cell death and consequent reduction in the vasculature. Adipose Homing Peptide (AHP) binds to the prohibitin (PHB) which is over expressed on the surface of the endothelial cells in the WAT vasculature of obese individuals. We previously demonstrated that AuNPs functionalized with AHP accumulated in the WAT of animal models of obesity. We evaluated the selective targeting and toxicity of AuNPs that were bi-functionalised with AHP and a pro-apoptotic peptide. We used 3 human cancer cell lines (Caco-2, MCF7 and HT29), of which only one (Caco-2) express PHB. Toxicity was evaluated using the WST-1 and the APOPercentage assays, while the uptake of the nanoparticles was confirmed by ICP-OES and TEM analysis. The cytotoxic activity of the receptor targeted AuNPs was more pronounced in the cells expressing the receptor for AHP. The AuNPs induced significant levels of apoptosis Caco-2 cells, but not MCF7 and HT29 cells. This study shows receptor mediated targeting, and the selective destruction of cells expressing the receptor through the induction of apoptosis and the potential for the development of targeted anti-angiogenic strategy.
# Thermosensitive nanogels with multiple anti-tumour associated effects

Wednesday, 28th September - 16:25 - Targeted drug delivery and Nanocarriers - Nanomedicine for cancer diagnosis & therapy - Amphitheatre 25 - Oral presentation - Abstract ID: 89

Dr. Malou Henriksen-Lacey¹, Mr. Malte Strozyk², Dr. Susana Carregal¹, Prof. Mathias Brust², Prof. Luis Liz-Marzán¹

1. CIC biomaGUNE, 2. University of Liverpool

Most chemotherapeutic treatments involve combination therapy to inhibit tumour growth, yet the systemic method of administration involving separate drug formulations results in undesirable side effects with a complicated dosing strategy. Consequently, the ability to combine multiple drugs in a single nanoparticulate delivery system in which release is stimulated by external stimuli, thereby diminishing systemic effects, is of interest. With this in mind we have explored thermosensitive microgels for their ability to change in diameter and volume in response to a change in temperature between 30 – 50°C. Within these vehicles we include gold nanoparticles, which can be used for Raman sensing or local heating, and two drugs of interest for tumour treatment, doxorubicin and pomalidomide. The effects of doxorubicin are well documented whereas pomalidomide remains a relatively novel drug that has shown promising results in the treatment of multiple myeloma (MM), among other actions including the inhibition of angiogenesis and anti-inflammatory cytokines secretion.

Gold nanoparticles, doxorubicin and pomalidomide were all entrapped within the microgels, after which positive or negatively charged polyelectrolytes were used as a coating material to stabilise the particles. In vitro experiments using single, double or unloaded microgels were conducted to assess the cytotoxic and antiangiogenic effects of doxorubicin and pomalidomide respectively. The EPR effect of cancer cells was investigated using an in vitro mixed cell co-culture model.

Collapsed states of the microgels were observed at biological relevant temperatures which led to release of both doxorubicin and pomalidomide. Encapsulation did not affect the activity of the drugs. In vitro studies with a variety of cell lines showed high levels of microgel uptake and a slower release compared to free drug. Pomalidomide was shown to have various effects including the inhibition of LPS-induced cytokine production, disregulation of the actin cytoskeleton and inhibition of angiogenesis in tube-formation assays.

We show that these thermosensitive microgels are highly flexible tools for the encapsulation of a variety of drug or sensing compounds which importantly remain active upon release. Due to the inclusion of gold nanoparticles the microgels also can be used as sensing particles or be stimulated with light to induce local heating.
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# Promising New NanoTheranostic Quantum dots Based on Ag2S-PEG-FA

Wednesday, 28th September - 16:50 - Targeted drug delivery and Nanocarriers - Nanomedicine for cancer diagnosis & therapy - Amphitheatre 25 - Oral presentation - Abstract ID: 568

Prof. havva yagci acar¹

1. Koc University

## Introduction

There is a tremendous effort in the development of nontoxic QDs emitting in the near-infrared (NIR) for medical use, both as an optical imaging and as gene/drug delivery agents. This provides an opportunity for deep tissue penetration and reduction of the tissue autofluorescence. Other important issues in practical application of QDs are the overall size, blood circulation, molecular targeting and stability. Ag2S quantum dots emerged as the most promising QDs in the last 5 years. They emit in the NIR region and are highly cyto-hemocompatible. Here, we report the first direct and size tuned synthesis of PEG coated Ag2S NIR QDs and their theranostic use for optical imaging and folic acid (FA) targeted DOX delivery to HeLa cells.

## Methods

Ag2S, Na2S and MPEG-SH were dissolved in DIW under Argon at different ratios and at pH values between 3 or 7.5. Reactions were run at 90°C. For FA tagging 30mol% HOOC-PEG-SH was used in the coating and FA was conjugated with EDC/NHS. DOX.HCl was loaded to QDs at pH 6. MTT assay was used to analyze cytotoxicity of particles in HeLa and NIH-3T3 cells.

## Results and Discussions

In the size and emission tuning of QDs, increasing Ag/S decreased the particle size. Synthesis at acidic pH produced larger QDs with stronger emission. Increasing PEG molecular weight from 2000 Da to 5000 Da increased the QY about 40 % with 43 nm red shift in peak maxima. All QDs were below 50 nm. Between 5-25 µg/mL Ag dose, no significant cytotoxicity was observed in either cell lines. Folate targeted and DOX loaded particles delivered DOX to the cells more effectively than untagged QDs. About 50% drop in the viability with only 15 nM DOX was achieved with FA targeting.

## Conclusions

Here, in a simple one step aq. synthesis, PEGylated Ag2S NIR QDs were synthesized with emission maxima between 847-930 nm with QY as high as 65 %, using HS-PEG coating. These particles were non-toxic to HeLa and NIH/3T3 even at mg range. Internalized particles, generates a strong NIR signal. FA-tagged particles showed excellent drug efficiency coupled with receptor mediated uptake.
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Study of RNA interference mediated by lipid-coated
calcium phosphate nanoparticle transfection in
high-grade gliomas.

Wednesday, 28th September - 17:15 - Targeted drug delivery and Nanocarriers - Nanomedicine for cancer diagnosis & therapy - Amphitheatre 25 - Oral presentation - Abstract ID: 140

Dr. Laura Pandolfi ¹, Dr. Miriam Colombo ¹, Mrs. Benedetta Santini ¹, Mrs. Lucia Salvioni ¹, Dr. Svetlana Avvakumova ¹, Prof. Silvia Nicolis ¹, Prof. Davide Prosperi ¹

1. University of Milano - Bicocca

Gliomas are the most prevalent brain tumor types in adults. The recent discoveries in gene regulation in-volved in gliomas initiation and development allowed for the generation of a new therapeutic approach using a sequence-specific gene silencing to alter gene activities.1,2

Calcium phosphate nanoparticles (CaP NPs) have been shown to be an attractive tool for non-viral gene delivery thanks to their biocompatibility and biodegradability. Indeed, calcium ions form complexes with the nucleic acid backbone, thus stabilizing RNA and DNA structures and, therefore, avoiding their fast degradation by nu-cleases.3,4

As a model platform useful for the investigation of gene silencing in gliomas, we synthesized CaP NPs for siRNAs delivery in GFP-expressing T794 oligodendroglioma cells isolated from transgenic new born mice induced with high grade brain tumor. This ex vivo model allowed us to study the efficiency of anti-GFP siRNA delivery follow-ing GFP fluorescence relating to the relevant gene expression. In order to enhance the circulation half-life in future in vivo experiments and to allow the functionalization of CaP nanoparticles, the core was coated with a cationic asymmetric lipidic bilayer (LCP NPs). LCP NPs were characterized by transmission electron microscopy (14.3±1.8 nm), dynamic light scattering and zeta-potential, showing a mean hydrodynamic diameter of 33.2±3.8 nm and a mean zeta-potential of +31.95±1.3 mV.

Interestingly, both control (empty LCP NPs) and siRNA-loaded LCP NPs did not exert a cytotoxic effect on T794 cells in 0.2-7 nM concentration. Flow cytometry analysis showed huge internalization of LCP NPs (2 nM) by cells up to 24 h of incubation. Treatment with siRNA-loaded LCP NPs confirmed their efficiency by decrease of the mean fluorescence intensity of GFP signal. On the basis of these promising results, we can translate this non-viral gene delivery system to alter other oncogenes supposed to play a pivotal role in gliomas initiation and development, including Sox2.
# Nanometronomics: doxorubicin-loaded H-ferritin allows for tailored treatment of breast cancer based on lower doses and higher safety

Wednesday, 28th September - 17:40 - Targeted drug delivery and Nanocarriers - Nanomedicine for cancer diagnosis & therapy - Amphitheatre 25 - Oral presentation - Abstract ID: 106

Dr. Serena Mazzucchelli¹, Ms. Michela Bellini², Dr. Marta Truffi¹, Dr. Luisa Fiandra¹, Ms. Maria Antonietta Rizzuto², Dr. Luca Sorrentino¹, Dr. Fabio Corsi¹, Prof. Davide Prosperi²

1. Università degli Studi di Milano, 2. Università degli Studi di Milano-Bicocca

The standard clinical approach for cancer chemotherapy is based on the concept of maximum tolerated dose (MTD) of drug for a patient. This strategy, however, presents several disadvantages, including major systemic toxicity and development of therapeutic resistance due to prolonged time between treatments. In addition, solid tumors are less effectively eradicated by MTD-based treatments because their proliferation is also sustained by the host microenvironment. Metronomic chemotherapy is based on more frequent and lower dose drug administrations compared to MTD [1]. Although initially intended to prevent angiogenesis, recent evidence suggests that potential new mechanisms of action are involved, including restoration of anticancer immune response and induction of tumor dormancy. For this reason, metronomic chemotherapy has gradually gained interest among clinicians for either primary systemic or maintenance therapy. However, low drug accumulation at the tumor and poor effectiveness against highly aggressive metastatic cancer limit the clinical application. To improve the efficacy of metronomic chemotherapy, we have investigated the potential of recombinant H-ferritin (HFn) nanocages targeted delivery of lower doses of chemotherapeutic drugs, such as doxorubicin (DOX). HFn was produced by genetic engineering and loaded with DOX [2]. Mice bearing a highly aggressive, DOX-resistant 4T1 breast cancer cells were treated in parallel with placebo, free DOX and HFn-DOX and monitored until day 21 at repeated doses as low as 1.24 mg/Kg, significantly lower than the minimal clinical dosage (2.4 mg/kg). Our results highlight that metronomic treatment with nanoformulated DOX is able to inhibit cancer growth with a lower dose and a significant improvement in DOX toxicity profile, even in a highly metastatic DOX-resistant breast cancer model in vivo. Histological analysis of cardiac tissues suggested that HFn-DOX allows overcoming cardiotoxicity, one of the most severe side effects of DOX. In conclusion, HFn-DOX has a tremendous potential for the development of novel nanometronomic chemotherapy for the next generation of safe and tailored oncological treatments.
# Turn-on and Color-changeable Fluorogenic Sensor Created by the 10BASEd-T

Wednesday, 28th September - 16:00 - Biological & medical nanodevices and biosensors - Tower 24 - Room 101 - Oral presentation - Abstract ID: 151

Prof. Masumi Taki¹

1. The University of Electro-Communications (UEC)

6-propionyl-2-(dimethylamino)naphthalene (PRODAN) is a well-known solvatochromic fluorescence reporter which shows both turn-on and color-changing effect when exposed in a hydrophobic environment [1]. Because of its extreme small size with lipophilic/nonionic property, we hypothesize that it can be interacted weakly with various hydrophobic pockets of most target biomacromolecules such as proteins. A conjugation of an appropriate surrounding structure to PRODAN core would strengthen both specificity and affinity to the target along with the solvatochromism, and consequently create a target-specific fluorogenic sensor.

Meanwhile, we have established a library construction system, namely gp10 based-thioetherification (10BASEdT), by conjugation between artificial molecules as the cores and randomized library peptides as the surroundings [2, 3]. By using this system, PRODAN core was conjugated with randomized peptide library on T7 phage, and selection against a target protein (glutathione-S-transferase; GST) was performed. After several rounds of biopanning, a consensus sequence of NTVSC\*HGF (C* represents PRODAN-hybridized Cys) was found. The chemically synthesized hybrid showed intense blue-cyan fluorescence only when GST was present. After the addition of excess amounts glutathione (GSH; a natural ligand of GST), it disappeared and weak yellow fluorescence appeared instead. Detailed results such as obtaining dissociation constant, thermodynamic parameters, and epitope-mapping upon the specific interaction between the hybrid sensor and GST will also be presented [4].

[1] Weber, G., Farris, FJ. (1979) Biochemistry, 18, 776-9.

[2] Fukunaga, K., Hatanaka, T., Ito, Y., Taki, M. (2013) Mol. BioSyst., 9, 2988-91.

[3] Fukunaga, K., Hatanaka, T., Ito, Y., Minami, M, Taki, M. (2014) Chem. Commun., 50, 3921-3; inside cover article.

[4] Taki, M., Inoue, H., Mochizuki, K., Yang, J, Ito, Y. (2016) Anal. Chem., 88, 1096-99 (2016).
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# One-step DNA Detection through Dual-Color Confocal Analysis of DNA-Assembled Scattering Nanoparticles: Case of a Fragment of Sesame

Wednesday, 28th September - 16:25 - Biological & medical nanodevices and biosensors - Tower 24 - Room 101 - Oral presentation - Abstract ID: 265

Dr. Stephanie VIAL¹, Mr. Youri BERRAHAL¹, Dr. Marta PRADO², Dr. Jérome WENGER¹

1. Institut Fresnel, Aix Marseille Université, 2. International Iberian Nanotechnology Laboratory

DNA-based detection methods have many applications in clinical diagnosis, food science, and environmental control. However, most of the analytical techniques currently in use, such as PCR, are expensive, cumbersome, and time consuming. Thus, methods such as enhanced surface plasmon resonance and fluorescence-based devices have been developed, but still present some limitations. Here, we present a novel approach based on Photon Cross Correlation Spectroscopy (PCCS) of metallic nanoparticles (NPs) that provides increased sensitivity, rapid detection and reduced cost in order to open new and breakthrough features for diagnosis.

The detection of DNA relies on the self-assembly of two distinct scattering NPs (silver nanoparticles (AgNPs) and gold nanorods (AuNRs)) mediated via specific-based pair recognition between ss-DNA target and ss-DNA probes anchored to NPs. Upon illumination using two laser beams, the particles passing through the volume of analysis are excited and their resulting scattered lights are collected and analyzed together. In the absence of target, both NPs move independently and their signals are not correlated. However, as the presence of DNA target initiates aggregation of the NPs through hybridization, AgNPs and AuNRs thus diffuse simultaneously. Consequently, their scattering responses are correlated, giving rise to temporal coincidence (Figure 1).

To validate the developed proof-of-concept PCCS, the study focused on the detection of a specific fragment of sesame, an allergenic food ingredient. Here, we have considered several aspects with the aim to improve detection efficiency within the shortest time. The optimization of the hybridization events between a target DNA and its probes, the sensitivity with quantitative analysis and the specificity to particularly distinguish perfectly matched DNA from defective one have been investigated. This new detection method was able to in-situ detect and quantify specific DNA sequence of sesame in a range of concentration from 5 pM to 1.5 nM, with LOD of 1 pM, after 30 min of incubation at 65°C. In addition, we have observed a net decrease in the detection when using one single matched and one deleted base in the sequence of the target and no possible detection in presence of non-complementary DNA
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# Up-converting and down-converting nanoparticle-based aptasensor model for multiplex detection of foodborne pathogens

Wednesday, 28th September - 16:50 - Biological & medical nanodevices and biosensors - Tower 24 - Room 101 - Oral presentation - Abstract ID: 375

Dr. Hasan Kurt¹, Dr. Meral Yüce¹, Mr. Babar Hussain¹, Prof. Hikmet Budak¹

1. Sabancı University

Advances in the selection of synthetic affinity agents, called aptamers, have provided us with the opportunity to develop highly efficient and specific biosensors. Coupling of aptamers with fluorescent nanoparticles have led to the rapid detection of various molecules from ions to disease biomarkers (1). However, only one type of nanoparticle has been used in these multiplexed assays. Herein, we report a multiplex sensing method based on the unique excitation profiles of two down-converting quantum dots (QD) and two up-converting nanoparticles (UCNP) that are coupled with the target-specific DNA aptamers. Our group recently reported the dual-excitation strategy for detection of Salmonella typhimurium and Staphylococcus aureus (2). In the current work, the dual excitation method is extended to detection of four foodborne pathogens, S. typhimurium, S. aureus, Listeria monocytogenes, and Pseudomonas aeruginosa.

Pathogen-specific aptamers were coupled with CdTe QDs at 510 and 720 nm emissions, and NaYF4-based UCNPs at 545 and 800 nm emissions. The resulting nanoparticles were conjugated with the magnetic beads that were previously coupled with the corresponding partial complementary DNA sequences. These conjugates were simultaneously used as molecular recognition elements for the detection. Partially complementary ssDNA-decorated magnetic beads were employed in the sensing system for separation purposes. Aptamer coupled QD and UCNP conjugates, and cDNA decorated magnetic beads were characterized using UV-Vis spectroscopy, Circular Dichroism, and Dynamic Light Scattering techniques. The multiplex detection of the selected food-borne pathogens was implemented using consecutive 335 nm and 980 nm excitations that eliminated the signal overlap between the nanoparticle species. Dual-excitation multiplex aptasensor enabled detection of the targets at trace amounts. The absence of signal cross-talk between the nanoparticles rendered multiplex detection of foodborne pathogens, offering a promising alternative to the conventional methods for detection.

Acknowledgment This project is supported by The Scientific and Technological Research Council of Turkey, TUBITAK Grant ID: 114Z379 (Cost Action CM1403).

1. Yüce, M., Ullah, N. & Budak, H. Trends in aptamer selection methods and applications. Analyst 140, 5379-5399 (2015).

2. Kurt, H., Yüce, M., Hussain, B. & Budak, H. Dual-excitation upconverting nanoparticle and quantum dot aptasensor for multiplexed food pathogen detection. Biosens. Bioelectron. 81, 280-286 (2016).
Analysis of the controlled drug release (CDR) from
biopolymer nanoparticles during the initial burst using
a novel modeling method

Wednesday, 28th September - 17:15 - Biological & medical nanodevices and biosensors - Tower 24 - Room 101 - Oral presentation - Abstract ID: 141

Ms. Cristiana de Azevedo ¹, Dr. Moritz Von Stosch ², Prof. Rui Oliveira ¹

1. Universidade Nova de Lisboa, 2. University of Newcastle

In the initial stage of the controlled release of a drug from a nanoparticle into a medium a phenomenon referred to as burst can occur. During burst a large amount of drug is released over a small period of time. Apart from the loss in the overall CDR time of actuation, high initial drug release rates can result into toxic drug levels, which would not be attained otherwise. The initial burst has been studied in the past, but with little success in elucidating the mechanisms that control the phenomenon.

In this contribution, a mathematical model is established to investigate how experimental conditions and nanoparticle formulations impact on the initial burst release. Experimental conditions, nanoparticle formulations and drug release profiles were extracted from publications for drug-PLGA or -PLGA/PEG carriers and a database was created. Subsequently, statistical methods were utilized to analyze the data and a model was developed that can predict the burst release based on experimental conditions and nanoparticle formulations.

Good agreement between model predictions and experimental burst data was obtained. Further analysis revealed that a clear augmentation in the quantity and kinetics of the burst is obtained when PEG is bound to PLGA. It also seems that an increase in the burst release quantity occurs for greater carrier particles, i.e. going from 5E1 nanometers towards microparticles, but at a slower rate. Longer chains of PLGA and smaller drug molecules show an enhancement on the burst rate.

The increased understanding of the burst release can in future be used to manipulate the system more rationally e.g. to reduce the intensity of the burst release.
Detection of cytokines using biosensor based on
functionalized nanocarriers growth on gold electrode

Wednesday, 28th September - 17:40 - Biological & medical nanodevices and biosensors - Tower 24 - Room
101 - Oral presentation - Abstract ID: 96

Prof. Abdelhamid Errachid ¹, Ms. Sahar Chaibi ², Dr. toufik hadjersi ³, Mr. Abaidia Seddik El Hak ²,
Dr. Abdoullatif Baraket ¹, Prof. Joan Bausells ⁴, Dr. yaakoubi nourdin ⁵, Dr. Nadia Zine ¹

1. Université Claude Bernard Lyon1, 2. Université M'hamed Bouguera, 3. Unité de recherché UDTS/CRTSE, 4. Instituto de Microelectronica de Barcelona, IMB-CNM (CSIC), Campus UAB, 08193, Bellaterra, Spain., 5. Université du Maine

Nowadays, it is well established that the performance of biosensors depends greatly on the influence imposed on biomolecules by immobilisation. In this context, the use of nanomaterials for the construction of biosensing devices constitutes one of the most interesting approaches. Application of gold nanoparticles in biosensing has attracted considerable attention because gold nanoparticles (GNPs) are the most compatible nanomaterial for preparation of engineered nanoplatforms in smart sensing devices beside the fact that they have interesting electronic, optical, thermal and catalytic properties. however, their application is obviously affected by many conditions, such as the size, the shape, the roughness of the substrate and the density of the particules on the surface. In this work we will present some phenomena noticed linked to the impact of gold Np characteristics's on the sensing of cytokine. The cytokine studied for this purpose were : IL1β, IL-10 and IL-6, using a spectroscopy of impedance for the detection of their corresponding antigens in the range of 1 pg/ml to 30 pg/ml. Effect such as antibodies/Np ratio on the sensing was noticed and this has affected really the sensibility of the biosensor. The gold nanoparticles were electrochemicaly synthesized from KAu(CN)2 diluted into phosphate buffer (PBS) at acidic bath using the chronoamperometric.

The sensitivity of Au-nanoparticles modified biosensor was more pronounced than gold biosensor. The limit of detection (LOD) of the gold biosensor was 1.5 pg/mL. The plot was linear (r=0.9968) within the range of 1-15 pg/ml. Whist the LOD of Au-nanoparticles modified biosensor was 1 pg/mL. The sensitivity of this modified biosensor was enhanced by the introduction of gold nanoparticules as electrolyte/gold interface has been increased. This biosensor is very promising for a rapid and sensitive detection.
# Bio-compatibilised carbon nanotubes display significant anti-tumoral effects in solid melanomas

Wednesday, 28th September - 16:00 - Nanomedicine for cancer diagnosis & therapy - Tower 24 - Room 103 - Oral presentation - Abstract ID: 302

Dr. Mónica L. Fanarraga¹, Mrs. Eloisa González-lavado¹, Dr. Lorena García-hevia¹, Ms. Carmen Pesquera¹, Mr. Fernando González¹, Dr. Jesús González¹, Dr. Juan C Villegas¹, Dr. Rafael Valiente¹

1. Universidad de Cantabria-IDIVAL

Multiwalled Carbon Nanotubes (MWCNTs) penetrate inside cells binding microtubules and interfering with the cellular biomechanics (1,2). This interaction leads to the formation of biosynthetic tubulin polymers (3,4) that display an enhanced stability, and this triggers anti-proliferative (4,5), anti-migratory (6) and cytotoxic (7) effects in different types of cancer cells, leading to antineoplastic effects in solid melanoma tumors (8) in murine models. Moreover, this intrinsic CNT antitumor activity is complementary -and synergetic- to that of traditional microtubule-stabilizing anticancer drugs such as taxol® (paclitaxel)(8). On the other hand, as is also the case for traditional cytotoxic chemotherapy, CNTs can also interfere with the function of heathy cells and produce many unwanted side-effects (9). Consequently, unless most concerns about the toxicity of these materials disappear, the development new treatments based on CNTs offer a poor risk-to-benefit ratio in oncology. Here we demonstrate how mild MWCNTs oxidative treatments enhance intracellular degradation by macrophages while still maintaining the ability of these nanomaterials to interfere with cellular cytoskeletal elements triggering anti-proliferative and cytotoxic processes in cultured cancer cells. Furthermore, we show how single intratumoral dosages of o-MWCNTs produce significant anti-tumoral effects in vivo, in solid malignant melanomas produced by allograft transplantation in murine recipients. We believe these findings have critical implications for the development of new CNT-based nanotherapies to overcome drug resistance in cancer among other applications.

## References

1L. García-Hevia, et al. Nanomedicine, 2014, 9, 1581-1588.

2C. Dong, et al. J. Mater. Chem. B, 2015, 3, 3983-3992.

3C. Z. Dinu, et al. Small, 2009, 5, 310-315.

4L. Rodriguez-Fernandez, et al. ACS Nano, 2012, 6, 6614-6625.

5L. M. Sargent, et al. Mutat. Res. - Genet. Toxicol. Environ. Mutagen., 2012, 745, 28-37.

6L. García-Hevia, et al. Adv. Healthc. Mater., 2015, 4, 1640-1644.

7L. García-Hevia, et al. Curr. Pharm. Des., 2015, 21, 1920-29.

8L. García-Hevia, et al. Adv. Healthc. Mater., 2016, 9:1080-7.

9K. Kostarelos, Nat. Biotechnol., 2008, 26, 774-6.
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# Targeting of TRAIL conjugated maghemite nanoparticles for biomedical applications

Wednesday, 28th September - 16:25 - Nanomedicine for cancer diagnosis & therapy - Tower 24 - Room 103 - Oral presentation - Abstract ID: 404

Mrs. Hanene BELKAHLA¹, Dr. Myriana Hemadi², Dr. Guillaume Herlem¹, Dr. Olivier Micheau³, Prof. Souad Ammar², Prof. Tijani Gharbi¹

1. Nanomedicine Lab, Imagery & Therapeutics, EA 4662, Université de Franche-Comté, Besançon, France., 2. ITODYS, Université Paris Diderot, SPC, CNRS UMR-7086, Paris, France, 3. INSERM, UMR866, Lipides nutrition cancer, Facultés de Médecine et de pharmacie, Université de bourgogne, Dijon, France

Iron oxide nanoparticles (NPs) have become increasingly important for cutting-edge applications in biomedicine due to their biocompatibility and attractive magnetic properties. Super-paramagnetic maghemite nanocrystals possess high magnetic susceptibility, low remanence, low coercivity, and high saturation magnetization. These properties make them ideal candidates for various biomedical applications including magnetic resonance imaging (MRI), magnetically controlled drug delivery (MCDD) and magnetic hyperthermia (MH) [1]. The use of these NPs for in-vivo diagnostics and/or therapy assays requires their coupling through covalent bonding to bioactive molecules (antibodies, enzymes, proteins, DNA, etc.).

TRAIL is a member of the tumor necrosis factor (TNF) superfamily. This protein is able to specifically bind to its receptor which is expressed at the surface of cancer cells and induces cell death [2].

Maghemite nanoparticles (MNP) with diameter of 10 and 100 nm were synthetized by the polyol method, coated with (3-aminopropyl)triethoxysilane (APTES)[3] and coupled to TRAIL protein. The ratio of TRAIL per NP was estimated by Prussian blue and protein assay. The magnetic properties were checked by SQUID before and after grafting. The efficiency of the targeting was checked in cellulo in different cell lines (HCT116, HEPG2, etc.). The resulting magnetic nanovector (NV) seems to be a good candidate for biomedical applications such as cancer therapy.

1. Laurent, S., et al., Magnetic fluid hyperthermia: focus on superparamagnetic iron oxide nanoparticles. *Adv Colloid Interface Sci*, 2011, 166(1-2): p. 8-23.

2. Zakaria, A.B., et al., Nanovectorization of TRAIL with single wall carbon nanotubes enhances tumor cell killing. *Nano Lett*, 2015, 15(2): p. 891-5.

3. Piraux, H., et al., Nanoparticles and the Transferrin-Receptor-1 iron-Acquisition Pathway – Synthesis, Kinetics, Thermodynamics and Cellular Internalization of a Holotransferrin-Maghemite Construct. *Biochim. Biophys. Acta.*, 2013. 1830 p. 4254–4264.
<figure><img src="image_37.png" /></figure>

Figure:

* Cell viability of HCT116 cells to TRAIL, non functionalized MNP and TRAIL functionalized MNP (MNP@TRAIL).

* Cell extracts from HCT116 cells stimulated or not with MNP,TRAIL or MNP@TRAIL were processes for immunoblot with antibodies to pro-caspase 3, pro-caspase 8, cleaved Lamin and cleaved PARP. Ponceau staining is shown below; Molecular weight markers are shown on the left handside.

* Transmission Electron Microscopy (TEM) of the produced MNP sized 5, 10, 100 nm.

Iconan 2016.jpg
# New promising Glucose-Metal Nanoparticles for potential applications in Radiotherapy

Wednesday, 28th September - 16:50 - Nanomedicine for cancer diagnosis & therapy - Tower 24 - Room 103 - Oral presentation - Abstract ID: 63

**Dr. Francesco Porcaro**¹, **Prof. Chiara Battocchio**², **Prof. Antonio Antoccia**¹, **Dr. Ilaria Fratoddi**³, **Dr. Iole Venditti**³, **Dr. Anna Fracassi**¹, **Dr. Igor Luisetto**¹, **Dr. Andrea Ugolini**¹, **Prof. Maria Vittoria Russo**³, **Prof. Giovanni Polzonetti**²

1. Roma Tre University, 2. University of Rome "Roma Tre", 3. Sapienza University of Rome

Recently, a novel approach in radiotherapy consists in the use of “High Z materials” for increasing radiation dose absorption in tumour site and therefore improving the effects of radiotherapy. Metal Nanoparticles (NPs) like Gold (Z=79) are the most promising candidate to become suitable materials for standard treatment in radiotherapy, due to their efficacy in delivering huge amounts of high Z atoms in the proximity of cancer cells nucleus [1]. Despite the great success of nanoparticles in this framework, there is still need to open new routes for the synthesis of more stable and functional nanoparticles.

The synthesis, characterization and assessment of biological response of innovative negatively charged functionalized metal nanoparticles is herein reported, for potential applications in the field of radiotherapy and drug delivery. Gold, Platinum and Silver nanoparticles (AuNPs, AgNPs, PtNPs, respectively) functionalized with two capping agents: the 3-mercapto-1-propansulfonate (3-MPS) and 1-β-thio-D-glucose (TG), have been on purpose synthesized and fully characterized. Advanced characterization techniques including X-Ray Photoelectron Spectroscopy (XPS) were applied to probe the chemical structure of the synthesized nanomaterials. Z-potential and Dynamic Light Scattering measurements allowed assessing the nanodimension, dispersity, surface charge and stability of Nanoparticles. In order to investigate the nanoparticles-cells interaction and evaluate the uptake efficiency for AuNPs, Transmission Electron Microscopy (TEM) and Flame Atomic Absorption Spectroscopy (FAAS) were performed to the "in vitro" Human Salivary Gland (HSG) cell model. Moreover, a deep cytotoxicity evaluation was assessed by means of MTT and SRB assays [2]. Preliminary data on NPs radiosensibilization were obtained by means of clonogenenic assay. In conclusion, with the purpose of increasing the amount of metal atoms inside the cell we have optimized the synthesis for a new kind of biocompatible and stable negatively charged TG-functionalized nanoparticles. Such particles are very promising for radiotherapy application.

[1] Neshatian M., Yang C., Hegarty N., Chithrani B. D., Precious metals for Biomedical Applications, Chapter: 4, Woodhead Publishing Baltzer N., pp.87-106 2014

[2] Porcaro F., Battocchio C., Antoccia A., Fratoddi I., Venditti I., Fracassi A., Luisetto I., Russo MV., Polzonetti G. Colloids Surf B Biointerfaces. 2016
<figure><img src="image_38.png" /><figcaption>**Figure** Example of Gold Nanoparticle functionalized with TG and 3MPS. Glucose nanoparticles are internalized most by tumour cell due to glucose receptor overexpression.</figcaption></figure>
# Microfluidic synthesis and biological evaluation of photothermal biodegradable copper sulphide nanoparticles

Wednesday, 28th September - 17:15 - Nanomedicine for cancer diagnosis & therapy - Tower 24 - Room 103 - Oral presentation - Abstract ID: 269

Ms. Isabel Ortiz de Solorzano¹, Mr. Martín Prieto¹, Dr. Gracia Mendoza¹, Dr. Teresa Alejo¹, Dr.

Silvia Irusta¹, Dr. Víctor Sebastián¹, Dr. Manuel Arruebo¹

1. University of Zaragoza

## INTRODUCTION

Copper(II) sulfide(CuS) nanoparticles are semiconductor chalcogenides with electronic and optical properties. As plasmonic material, it absorbs near infrared light converting it into heat due to the excitation of direct and indirect transitions and plasmonic photoexcitation(1)(Smith, 2015). Those optical properties make them useful in a wide variety of biomedical applications.

Biodegradability and higher photothermal conversion efficiencies(1) are one of the main advantages of CuS nanoparticles compared to other plasmonic nanoparticles. Thus, Guo et al.,2013, demonstrated that plasmonic hollow gold NPs remained in the body one month after injection in BALB/c mice at high levels (> 96%) whereas, only a 10% of CuS NPs injected remained in the animals, being mostly excreted following the hepatobiliary route.

Polydispersity, low-yield and batch-to-batch inconsistencies are the main shortcomings when synthesizing NPs which can be overcome by using microfluidic reactors. In this work we have produced CuS NPs in a continuous process showing high photothermal efficiency as well as with the ability of generating reactive oxygen species(ROS).

## METHODS

CuS NPs were synthetized using a batch reactor at different temperatures following the work of Ramadan et al.2012. The continuous microfluidic synthesis was carried out by using two consecutive Y-shaped PEEK micromixers to produce sacrificial Cu2O NPs in a first step and CuS NPs in a second step. The NPs obtained were characterized by TEM, XRD, UV-vis, XPS and DLS. Photothermal effects and ROS generation were also evaluated. The NPs cytotoxicity and evaluation of cell apoptosis were performed on mouse mesenchymal stem cells, human dermal fibroblasts and THP1 human monocytes and macrophages.

## RESULTS AND DISCUSSION

It is possible to synthesize aqueous CuS NPs in a continuous manner showing absorbance in the near infrared region of the electromagnetic spectrum with a microfluidic reactor. The NPS physicochemical properties are similar to the ones obtained in the conventional batch synthesis although the synthesis times were reduced 4 fold when using the microreactor. Under simulated physiological conditions CuS nanoparticles degrade into soluble copper sulfates which highlights the advantage of those nanomaterials compared to other conventional plasmonic nanomaterials. Those NPs show at subcytotoxic doses an elevated photothermal effect as well as ROS generation.
# Development of Innovative Multistage Nanovectors for Cancer Immunotherapy

Wednesday, 28th September - 17:40 - Nanomedicine for cancer diagnosis & therapy - Tower 24 - Room 103 - Oral presentation - Abstract ID: 20

Ms. Flavia Fontana¹, Dr. Mohammad-Ali Shahbazi¹, Dr. Dongfei Liu¹, Dr. Hongbo Zhang¹, Mr. Ermei Mäkilä², Prof. Jarno Salonen², Prof. Jouni Hirvonen¹, Dr. Helder A. Santos¹

1. University of Helsinki, 2. University of Turku

## Introduction

Cancer is a very challenging disease and new approaches are sought to help in its treatment. Cancer immunotherapy involves the patient's own immune system in the fight against the tumors using antibodies directed to the so-called “check-point inhibitors” or by the administration of chimeric T-lymphocytes. It is envisaged that the administration of nanoparticles in cancer vaccines would lead to a decrease in the side effects of the drugs, helping targeted drug delivery, and increasing the efficacy of the therapy due to the adjuvant properties of the particles themselves [1].

Porous silicon (PSi) is a biocompatible porous material which presents adjuvant-like properties [2]. Acetylated dextran (AcDX) and spermine-modified AcDX (SpAcDX) are chemically-modified polymers derived from dextran, a FDA approved material, able to stimulate an immune response by the activation of Toll-like receptors [3].

## Methods

Multistage nanovectors were prepared by nanoprecipitation during glass capillary microfluidics [4]. PSi nanoparticles were encapsulated in a polymeric matrix made of AcDEX. The immunostimulant properties of the nanoparticles were assessed in vitro on human immortalized immune cells and blood-derived monocytes.

## Results

The developed nanosystems induced the expression of co-stimulatory factors (CD86) in, for example, peripheral blood monocytes (Fig.1).

## Discussion

The multistage nanovectors, produced with different polymers promoted the expression of CD86 from human monocytes, KG 1, and BDCM (results not shown). Moreover, the system modified with membranes derived from cancer cells showed the highest levels of CDs stimulation, with a statistically significant difference compared to the particles with the polymer alone.

## Conclusions

The developed systems promoted the expression of costimulatory factors (CD86) confirming their adjuvant properties that make them suitable platforms for further development as cancer vaccines.

## References

[1] D.J. Irvine, M.C. Hanson, 115 Chem Rev (2015), 11109-46.

[2] M.A. Shahbazi, T.D. Fernandez, Biomaterials 35 (2014), 9224-35.

[3] L. Cui, J.A. Cohen, Bioconjug Chem 22 (2011), 949-57.

[4] D. Liu, S. Cito, Adv Mater 27 (2015), 2298-304.
Acknowledgements

Financial support from the Academy of Finland (Grants No. 252215 and 281300), the University of Helsinki Research Funds, the Biocentrum Helsinki, and the European Research Council (FP/2007-2013, Grant 310892) is greatly acknowledged.

<figure><img src="image_39.png" /><figcaption>Cd 86 expression on pbmc.png</figcaption></figure>
Thrombolytic therapy based on P-selectin targeted
polymer nanoparticles

Wednesday, 28th September - 16:00 - Targeted drug delivery and Nanocarriers - Nano-Imaging for diagnosis, therapy and delivery - Tower 24 - Room 105 - Oral presentation - Abstract ID: 378

Ms. Maya Juenet ¹, Ms. Rachida Aid-launais ¹, Dr. Véronique Ollivier ¹, Ms. Alice Berger ¹, Mr. BO LI ¹,
Dr. Didier Letourneur ¹, Dr. Cédric Chauvierre ¹

1. INSERM U1148

Injection of tissue plasminogen activator (tPA) remains the standard treatment for thrombolysis. However, high doses have to be administered with the risk to cause hemorrhages [1]. In our work, tPA-loaded nanoparticles were investigated to decrease the injected amount while keeping the same efficiency by ensuring a specific delivery. For this purpose, we synthesized polymer nanoparticles (NPs) targeting P-selectin as this protein is indeed a biomarker of activated platelets composing the thrombus.

Fluorescent polysaccharide-poly(isobutylcyanoacrylate) nanoparticles were synthesized by redox radical emul-
sion polymerization. They were functionalized with fucoidan (Fuco-NPs) as this anionic polysaccharide shows
a nanomolar affinity for P-selectin [2]. Carboxymethyl-dextran was used as control (Control-NPs). To validate
the targeting strategy, an in vitro flow assay was set up. Human whole blood was injected into collagen coated
micro-channels to induce platelets activation and aggregation. Adhesion of NPs onto aggregates in venous con-
ditions was quantified by fluorescence microscopy. tPA (Actilyse®) was loaded onto NPs by adsorption. The
drug-related activity of the suspensions was measured in vitro and used to normalize the doses. Finally, an in
vivo study was performed in a mouse model of mesenteric thrombosis induced by iron chloride.

Both NPs were similar in size, surface charge and fluorescence intensity. Fuco-NPs bound significantly more
than Control-NPs to platelet aggregates confirming the potential of Fucoidan-functionalized NPs for targeting
activated platelets (Figure A). The preclinical study showed that only Fuco-NPs-tPA were able to induce complete
thrombolysis (4 mice out of 11) at a dose four times lower than the recommended one for murine models (Figure
B). This result reinforced the choice of an active targeting approach, compare to other delivery strategies [3].

Fuco-NPs were efficient as carriers for thrombolysis enabling to decrease the injected tPA dose and therefore
the risk of undesirable side effects. Although the variability between individuals is high in the developed model,
this study underlines the great potential of targeted nanomedicine to fight thrombotic diseases.

[1] J. Alvazer-Sabin et al. Lancet Neurology 12(7) (2013)

[2] L. Bachelet et al. Biochimica et Biophysica Acta 1790(2) (2009)

[3] M. Varna et al. Future Science OA 1(4) (2015)
A *In vitro* flow assay (venous conditions)

<figure><img src="image_40.png" /></figure>

**Figure A) In vitro flow assay.** Platelets were labeled in green and NPs in red. Human whole blood was first injected at 67.5 dyn/cm². Once aggregates were formed, NPs were injected at 6.75 dyn/cm². After 5 minutes, the Mean Fluorescence Intensity (MFI) of NPs was normalized to the platelets MFI per aggregate. One dot represents one aggregate (n=3 channels per condition, **p<0.001).

B *In vivo thrombolysis on mouse mesenteric vein*

<figure><img src="image_41.png" /></figure>

**B) In vivo thrombolysis study.** An iron chloride solution was left for 1 minute on an exposed mesenteric vein to cause thrombosis. Around 10 minutes after thrombus induction, free IPA, NPs-IPA or NPs alone were injected. Platelets were fluorescently labeled and the thrombus area was monitored over 30 minutes by fluorescence microscopy. Treatment efficacy at 2.5 mg/kg was compared to the recommended dose of 10 mg/kg. 0% means total recanalization. One dot represents one mouse (p<0.05).
# Uptake and intracellular localization of engineered gold nanoparticles in A549 cells.

Wednesday, 28th September - 16:25 - Targeted drug delivery and Nanocarriers - Nano-Imaging for diagnosis, therapy and delivery - Tower 24 - Room 105 - Oral presentation - Abstract ID: 380

Ms. Abiola Dosumu¹, Ms. Shani Osborne¹, Prof. Zoe Pikramenou¹, Dr. Nik Hodges¹

1. University of Birmingham

Engineered gold nanoparticles (AuNPs) possess unique physiochemical properties such as large surface area to mass ratio. Their relative ease of synthesis including incorporation of transition metal-ligands and other labelling agents produces functionalized AuNPs with potential applications for therapeutic and diagnostic medicine. Prior to clinical applications, an understanding of uptake, localization and cellular fate in relation to surface properties is critical.

In the current study we synthesised 13 nM AuNPs coated with a luminescent ruthenium metal complex to give rise to RubpySS.AuNP13. A non-toxic concentration (0.9 nM, MTT assay) was chosen to study particle uptake in A549 human lung carcinoma cells. Cells were incubated with RubpySS.AuNP13 (2-72 hours), uptake and co-localisation with different cellular compartments was studied using fluorescent probes (mitochondria, endoplasmic reticulum and golgi) and GFP-tagged organelle markers (early endosome, lysosome and autophagosome) by confocal microscopy supplemented by transmission electron microscopy (TEM). The size of particles in cells as quantified by TEM was in agreement with characterisation data of particles in solution (DLS and TEM) and particle size remained unchanged after up to 72 hours incubation indicating that RubpySS.AuNP13 does not aggregate upon cellular uptake. Particle number increased over time and this was confirmed by quantitative analysis (ImageJ) and further supported by TEM.

Both confocal and TEM images suggested the involvement of the endo-lysosomal pathway as a route of cellular uptake of RubySS.AuNP13 in A549 cells. RubySS.AuNP13 showed no significant co-localisation with markers for mitochondria, endoplasmic reticulum and the golgi and this was confirmed by TEM. In contrast, we observed particles in the early endosomes at early time points (4 hours), after 12-72 hours particles were observed to accumulate in a time-dependent manner in both the lysosomal and autophagosomal compartments of the cell, again this was confirmed by TEM. Also evident was a change in diffuse cytoplasmic LC3 staining to more punctuate staining consistent with the formation of active autophagosomes. In conclusion, we report that a major route of cellular uptake of RubySS.AuNP13 is by the endo-lysosomal pathway and furthermore accumulation of particles into autophagosome may have important implications for their cellular fate and toxicity.
<figure><img src="image_42.png" /><figcaption>RubySS.AuNP13 in A549 cells after 24hours</figcaption></figure>

<figure><img src="image_43.png" /></figure>

TEM images of RubySS.AuNP13 in water and after 24hours incubation in A549 cells shows
particles remains the same upon cellular uptake.

Rubyss bbb.png
# Development of polymer microcapsules functionalized with fucoidan to target P-selectin under arterial flow conditions

Wednesday, 28th September - 16:50 - Targeted drug delivery and Nanocarriers - Nano-Imaging for diagnosis, therapy and delivery - Tower 24 - Room 105 - Oral presentation - Abstract ID: 352

Mr. BO LI¹, Ms. Maya Juenet¹, Dr. Véronique Ollivier¹, Ms. Rachida Aid-launais¹, Dr. Didier Letourneur¹, Dr. Cédric Chauvierre¹

1. INSERM U1148

## Introduction

The lack of technology, which enables early detection and predicts rupture risk of aneurysms hampers their diagnosis and treatments. P-selectin expressed by activated platelets and endothelial cells is a marker of biologically active arterial thrombi. Our group previously demonstrated that fucoidan-based systems could target P-selectin to detect platelet and endothelial activation in vivo. Therein, microcapsules (MCs) made of poly-cyanoacrylate and polysaccharide and functionalized with fucoidan (Fuco-MCs) were designed as new carriers to target P-selectin. Flow channels were used to visualize and quantify the affinity of Fuco-MCs for P-selectin and for human activated platelet aggregates expressing P-selectin in arterial flow conditions.

## Methods

Microcapsules (MCs) were synthesized by anionic emulsion polymerization in alkaline condition. Vena8 Fluoro+ chambers were coated with a solution either with recombinant P-, E- or L-selectin. Some channels had different doses of P-selectin to confirm that the affinity of Fuco-MCs for P-selectin was depended on the concentration of P-selectin. A suspension of MCs or Fuco-MCs was passed through the channels (shear stress 1,500 s⁻¹). In the second experiment, whole human blood was firstly injected into collagen coated channels to induce platelets activation and aggregation, subsequently MCs or Fuco-MCs were injected in the channels, visualized and quantified under fluorescence microscopy.

## Results

In this work we, developed microcapsules with a new alkaline solvent emulsion-evaporation polymerization process, confirmed their hydrodynamic diameter distribution, the microcapsule structure and the presence of the targeting agent, fucoidan, at the surface. Fuco-MCs bound significantly more to the P-selectin coating than MCs, and results indicated a dose-dependent effect while increasing the concentration of P-selectin. Furthermore, their adhesion to L- and E-selectins was significantly lower than to P-selectin. Moreover, only Fuco-MCs accumulated at the surface of platelet aggregates.

## Conclusion

This work gave the first evidence that microcapsules can be obtained according to one-step polymerization process of isobutyl cyanoacrylate in alkaline conditions. Subsequently, the microcapsule surface is functionalized by fucoidan, which gives microcapsules the property of specificity to P-selectin under blood flow conditions. Meanwhile, organic core is considered as a potential loading space of probe and/or hydrophobic drug for the diagnosis and/or the treatment of vascular diseases overexpressing P-selectin.
<figure><img src="image_44.png" /><figcaption>**Figure.** Microcapsule adhesion over platelet aggregates. Whole blood labeled with Diocé was injected onto channels coated with collagen at 50 µg/mL. Under arterial flow conditions, platelets adhered onto the channel wall forming aggregates expressing P-selectin (Left) and FITC-MCs or FITC-Fuco-MCs were then injected for 5 minutes (Middle panel). Channels were washed with 0.0% NaCl. Microcapsules and aggregates co-localization was assessed by merged fluorescence microscopy (Right).</figcaption></figure>

Bo li - iconan 2016.jpg
Ligand Tethered Gold Nanoparticles against Untamed
and Drug Resistant Leishmania Donovani

Wednesday, 28th September - 17:15 - Targeted drug delivery and Nanocarriers - Nano-Imaging for diagnosis, therapy and delivery - Tower 24 - Room 105 - Oral presentation - Abstract ID: 326

Prof. Arup Mukherjee ¹, Mr. Asim Halder ¹, Dr. Suvadra Das ¹

1. University of Calcutta

Introduction: Gold nanoparticles (Aunp) are useful tools in chemical biology interfaces. Engineered Aunps in sub-100 nm ranges are often successful in cancer chemotherapy and infections control. We have designed quasi-spherical Aunps and functionalized them further in lactoferrin conjugation reactions. New generation ligand tethered nanoparticles were experimented successfully against Leishmania donovani parasites.

Methods: Green synthesis for highly mono-dispersed Aunps was carried out using gallic acid. Reductive formation of Aunps at 40C under sonication was monitored in Uv-Vis spectrophotometer and the particles recovered by centrifugation 16000 rpm, 30 min. Aunps were re-suspended in water and conjugated in 1,2 ethylenediamine. Human lactoferrin (Lf) was further functionalized onto Aunps in EDC-NHS reactions. Plasmonic Lf-Aunps uptake in mouse peritoneal macrophage was monitored and leishmanicidal efficacy against axenic and macrophage infested L. donovini AG 83 strains were studied in depth.

Results and Discussion: Aunps formation was monitored in Uv-Vis spectrophotometer (Fig 1). Aunps appeared quasi-spherical with average TEM size near 7.6 nm. The zeta potential was recorded at – 24.2 mv and PDI was 0.21. Lf-Aunps observed relative increase in size (Fig 1E) and the zeta potential was -18.2 mv. Diffractions indexed in XRD studies were at (1 1 1), (2 0 0), (2 2 0) and (3 1 1). Aunp and Lf-Aunp mass compositions were analyzed in ICP-EES. Lf-Aunps were exceedingly effective against both axenic and the macrophage resident amastigotes. Comparative evaluations under culture conditions confirmed Lf-Aunp IC50 4.1±1 µM and 2.0±0.3 µM against L. donovini AG83 axenic and macrophage infested forms. Lf-Aunps were equally effective against sodium stibogluconate resistant strains but were refractory in paromomycin resistant organisms. Nanoparticle remarkable efficacy against macrophage infested leishmania was reasoned due to targeted transport, rapid uptake and anti-oxidant potentials.

Figure 1: Nanoparticles synthesis and efficacy against L. donovini AG83.

Nanoparticle(Aunp) TEM,A; SAED,B; Plasmonic response in time scale, C; In different gallic acid concentrations, D; Lf-Aunp TEM studies,E; Lf-Aunp TEM uptake in macrophages (30 min exposure),F.
<figure><img src="image_45.png" /><figcaption>Figure 1.png</figcaption></figure>
Harnessing human blood to examine bio-nano
interactions at the cellular level

Wednesday, 28th September - 17:40 - Targeted drug delivery and Nanocarriers - Nano-Imaging for diagnosis, therapy and delivery - Tower 24 - Room 105 - Oral presentation - Abstract ID: 187

Mr. Joshua J Glass ¹, Ms. Liyu Chen ², Dr. Michael Whittaker ³, Ms. Sarah Mann ³, Prof. Edmund Crampin ¹, Dr. John Quinn ³, Ms. Ewa Czuba ³, Dr. Kristofer Thurecht ², Dr. Georgina Such ¹, Prof. Thomas Davis ³, Dr. Angus Johnston ³, Dr. Robert De Rose ¹, Prof. Stephen Kent ¹

1. University of Melbourne, 2. University of Queensland, 3. Monash Institute of Pharmaceutical Sciences

Aim: The capacity to predict interactions between nanomaterials and primary human cells will benefit the ra-
tional design of novel nano medicines. However, these fundamental relationships have not been adequately
defined. By examining various nanoparticle systems with fresh human blood cells, we aim to establish prin-
ciples of bio-nano interactions and generate predictive relationships that can inform the next generation of
nanomedicines.

Methods: Four separate nanoparticle technologies were used to generate particles with varying charge and surface chemistries. Each particle was incubated with fresh healthy human blood (to study cell association/uptake) or purified cells (to study immune activation) at 37°C and 4°C and analysed by flow cytometry.

Results: The properties of nanoparticles significantly impact immunological outcomes, including immunoactivation and leukocyte uptake.

a. Hyperbranched polymers (HBPs) differentially activate primary human blood dendritic cells based on charge. HBP of 8 nm diameter were prepared and tested under endotoxin-free conditions. Cationic (+16 mV) HBPs activated myeloid dendritic cells (DC; 51.7 ± 4.3% IL-8+) but not plasmacytoid DCs, while neutral and anionic (-15 mV) HBPs were not immunostimulatory to either subset.

b. Charge dictates HBP association with different immune cell subsets. Cationic HBPs associated with most cell types (monocytes, granulocytes, DCs and B cells), while at 37°C anionic HBPs preferentially associated with cells specialized for pathogen clearance and processing.

c. Nanoparticles engineered with terminal disulphides displayed enhanced association with human blood com-
ponents, particularly phagocytic cells and platelets, over control nanoparticles.

d. Altering the chemical composition of nanoparticle surfaces can reduce phagocytic clearance. Linear poly(ethylene)glycol surfaces reduced phagocytic uptake compared with brushed poly(ethylene)glycol of the same molecular weight.

Conclusion/Future: We are in the process of creating a nanoparticle ‘characteristic matrix’ to examine the effect of single nanoparticle characteristics on immunoactivation and blood interaction profiles. We will subsequently apply mathematical modelling principles to generate predictive models of bio-nano interactions.