# An overview of the data obtained during the validation of an optimized MALDI-TOF MS Biotyper database for the identification of anaerobic bacteria

A.C.M. C M Veloo, H. Jean-Pierre, U. S. Justesen, T. Morris, E. Urban, I.

Wybo, M. Kostrzewa, A. W. Friedrich

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A.C.M. C M Veloo, H. Jean-Pierre, U. S. Justesen, T. Morris, E. Urban, et al.. An overview of the data obtained during the validation of an optimized MALDI-TOF MS Biotyper database for the identification of anaerobic bacteria. Data in Brief, 2018, 18, pp.1484-1496. 10.1016/j.dib.2018.04.070. hal-03143042

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Data in Brief

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Data Article

# An overview of the data obtained during the validation of an optimized MALDI-TOF MS Biotyper database for the identification of anaerobic bacteria

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A.C.M. Veloo<sup>a,∗</sup>, H. Jean-Pierre<sup>b,c</sup>, U.S. Justesen<sup>d</sup>, T. Morris<sup>e</sup>, E. Urban<sup>f</sup>, I. Wybo<sup>g</sup>, M. Kostrzewa<sup>h</sup>, A.W. Friedrich<sup>a</sup>, on behalf of the ENRIA workgroup<sup>a,b,c,d,e,f,g,h</sup>

## ARTICLE INFO

**Article history:**

Received 12 March 2018

Received in revised form

10 April 2018

Accepted 18 April 2018

Available online 23 April 2018

## ABSTRACT

This data in brief article presents the data obtained during the validation of the optimized Biotyper Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) database. The validation was performed by the different expertise laboratories, collaborating within the European Network for the Rapid Identification of Anaerobes (ENRIA) project, using 6309 human clinical anaerobic bacterial strains.

Different databases were compared with each other; the db 5989 database (V5 database); the V5 database complimented with

<footnote><sup>a</sup> University of Groningen, University Medical Center Groningen, Department of Medical Microbiology, Groningen, The Netherlands</footnote>

<footnote><sup>b</sup> Centre Hospitalier Universitaire de Montpellier, Hôpital Arnaud de Villeneuve, Laboratoire de Bactériologie, 371 Avenue du Doyen Gaston Giraud, 34295 Montpellier Cedex 5, France</footnote>

<footnote><sup>c</sup> Université Montpellier 1, UMR5119 ECOSYM, Equipe Pathogènes Hydriques Santé Environnements, UMR 5569 Hydrosciences, UFR Pharmacie, 15 avenue Charles Flahault, 34093 Montpellier Cedex 5, France</footnote>

<footnote><sup>d</sup> Department of Clinical Microbiology, Odense University Hospital, Odense, Denmark</footnote>

<footnote><sup>e</sup> UK Anaerobe Reference Unit, Public Health Wales Microbiology, Cardiff, UK</footnote>

<footnote><sup>f</sup> Institute of Clinical Microbiology, University of Szeged, Hungary</footnote>

<footnote><sup>g</sup> Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Brussels, Belgium</footnote>

<footnote><sup>h</sup> Bruker Daltonics, Bremen, Germany</footnote>

<footnote>DOI of original article: https://doi.org/10.1016/j.anaerobe.2018.03.007</footnote>

<footnote>* Corresponding author.
E-mail address: a.c.m.veloo@umcg.nl (A.C.M. Veloo).</footnote>

<footnote>https://doi.org/10.1016/j.dib.2018.04.070</footnote>

<footnote>2352-3409/© 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).</footnote>
Main Spectral Profiles (MSPs) of ENRIA strains added to the next update of the database; and the V5 database complimented with the MSPs of all anaerobic clinical isolates collected within the ENRIA project. For a comprehensive discussion of the full dataset, please see the research article that accompanies this data article (Veloo et al., 2018) [1]

© 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license

(http://creativecommons.org/licenses/by/4.0/).

## Specifications table

<table><tr><td>Subject area</td><td>Medical Microbiology</td></tr><tr><td>More specific subject area</td><td>MALDI-TOF MS</td></tr><tr><td>Type of data</td><td>Table</td></tr><tr><td>How data was acquired</td><td>Biotyper, Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (Bruker Daltonics, Bremen, Germany)</td></tr><tr><td>Data format</td><td>Analyzed</td></tr><tr><td>Experimental factors</td><td>Assessment of the effect of the optimization of the Biotyper database for the identification of anaerobic bacteria was, by comparing the optimized database with the 'old' database.</td></tr><tr><td>Experimental features</td><td>Colonies of bacterial strains directly spotted on to a MALDI-TOF MS target plate and covered with matrix. If necessary, an on target extraction with 70% formic acid was performed prior to the addition of the matrix.</td></tr><tr><td>Data source location</td><td>Groningen, The Netherlands</td></tr><tr><td>Data accessibility</td><td>Provided with this article</td></tr></table>

## Value of the data

* Demonstrates how the Biotyper MALDI-TOF MS system performs for the identification of anaerobic genera commonly encountered in human clinical specimens.

* Highlights the performance of the Biotyper MALDI-TOF MS system with less commonly encountered genera/species of anaerobic bacteria (as it included a large number of isolates)

* Collaboration of specialist expertise laboratories yielded a MALDI-TOF MS database optimized for the identification of a significant number of anaerobic species.

## 1. Data

The data presented shows the performance of the system for the identification of anaerobic bacteria, prior to and after optimization of the database [1]. The obtained identification of each strain is categorized by genus. The log-score is used to assess the reliability of the identification. An increase in the log score was interpreted as a more reliable identification. Therefore the number of strains with a higher log score after optimization are also shown in Table 1.
<caption>**Table 1**</caption>

The MALDI-TOF MS data obtained during the validation of the MALDI-TOF MS Biotyper database.

<table><thead><tr><th>Strains (6309)</th><th>V5 database</th><th>V5 database + ENRIA (confirmed)</th><th>Higher score</th><th>Old database + ENRIA (all MSPs)</th><th>Higher score</th></tr></thead><tbody><tr><td></td><td>≤ 1.7</td><td>1.7–2</td><td>≥ 2</td><td>1.7–2</td><td>≥ 2</td></tr><tr><td>Acidaminococcus spp. (7)</td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>A. intestini (7)</td><td>1</td><td>6</td><td>1</td><td>6</td><td>5</td></tr><tr><td>Alistipes spp. (8)</td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>A. finegoldii (4)</td><td></td><td>4</td><td></td><td>4</td><td>4</td></tr><tr><td>A. onderdonkii (3)</td><td></td><td>3</td><td></td><td>3</td><td>3</td></tr><tr><td>A. indistinctus (1)</td><td>1</td><td></td><td></td><td>1</td><td>1</td></tr><tr><td>Alloscardovia spp. (16)</td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>A. omnicolens (16)</td><td>2</td><td>14</td><td>2</td><td>14</td><td>2</td></tr><tr><td>Atopobium spp. (58)</td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>A. minutum (6)</td><td></td><td>6</td><td></td><td>6</td><td>3</td></tr><tr><td>A. parvulum (25)</td><td>7</td><td>18</td><td>7</td><td>18</td><td>7</td></tr><tr><td>A. rimae (15)</td><td>1</td><td>12</td><td>2</td><td>12</td><td>8</td></tr><tr><td>A. vaginae (4)</td><td></td><td>4</td><td></td><td>4</td><td>1</td></tr><tr><td>'A. detroitii' (3)</td><td>3</td><td></td><td>3</td><td></td><td></td></tr><tr><td>Atopobium spp. (5)</td><td>5</td><td></td><td>5</td><td></td><td></td></tr><tr><td>Alloprevotella spp. (1)</td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>A. tannerae (1)</td><td>1</td><td></td><td>1</td><td></td><td>1</td></tr><tr><td>Bifidobacterium spp. (52)</td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>B. bifidum (3)</td><td></td><td>3</td><td></td><td>3</td><td></td></tr><tr><td>B. breve (15)</td><td>1</td><td>14</td><td>1</td><td>14</td><td>5</td></tr><tr><td>B. catenulatum (1)</td><td></td><td>1</td><td></td><td>1</td><td></td></tr><tr><td>B. dentium (13)</td><td>2</td><td>11</td><td>2</td><td>11</td><td>2</td></tr><tr><td>B. longum (16)</td><td>9</td><td></td><td>9</td><td>7</td><td>9</td></tr><tr><td>B. scardovii (4)</td><td></td><td>4</td><td></td><td>4</td><td></td></tr><tr><td>Bilophila wadsworthia (24)</td><td>7</td><td>15</td><td>2</td><td>2</td><td>5</td></tr></tbody></table>
<table><tbody><tr><td>Bulleidia extructa (3)</td><td></td><td>3</td><td></td><td>3</td><td></td><td>3</td></tr><tr><td>Butyricimonas spp. (1)</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>B. virosa (1)</td><td>1</td><td></td><td>1</td><td></td><td>1</td><td></td></tr><tr><td>Collinsella spp. (4)</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>C. aerofaciens (4)</td><td></td><td>4</td><td></td><td>4</td><td></td><td>4</td></tr><tr><td>Campylobacter spp. (48)</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>C. concisus (4)</td><td>1</td><td>3</td><td>1</td><td>3</td><td>1</td><td>3</td></tr><tr><td>C. fetus (1)</td><td></td><td>1</td><td></td><td>1</td><td></td><td>1</td></tr><tr><td>C. rectus (5)</td><td>2</td><td>1</td><td>2</td><td>2</td><td>3</td><td>3</td></tr><tr><td>C. showae (1)</td><td></td><td>1</td><td></td><td>1</td><td></td><td></td></tr><tr><td>C. hominis (1)</td><td></td><td>1</td><td></td><td>1</td><td></td><td>1</td></tr><tr><td>C. ureolyticus (34)</td><td></td><td>12</td><td>22</td><td>12</td><td>22</td><td>22</td></tr><tr><td>C. gracilis (2)</td><td>2</td><td></td><td>2</td><td></td><td>1</td><td>1</td></tr><tr><td>Cetobacterium spp. (1)</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>C. somerae (1)</td><td>1</td><td></td><td>1</td><td></td><td>1</td><td>1</td></tr><tr><td>Desulfovibrio spp. (6)</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>D. desulfuricans (4)</td><td>4</td><td></td><td>1</td><td>1</td><td>2</td><td>2</td></tr><tr><td>'D. fairfieldensis' (2)</td><td>2</td><td></td><td>2</td><td></td><td></td><td>2</td></tr><tr><td>Dialister spp. (69)</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>D. micraerophilus (21)</td><td></td><td>2</td><td>19</td><td></td><td>21</td><td>21</td></tr><tr><td>D. pneumosintes (48)</td><td></td><td>5</td><td>43</td><td>4</td><td>44</td><td>44</td></tr><tr><td>Dielma fastidiosa (2)</td><td>2</td><td></td><td>2</td><td></td><td></td><td>2</td></tr><tr><td>Eubacterium spp. (8)</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>E. brachy (3)</td><td>1</td><td>2</td><td>1</td><td>2</td><td>1</td><td>2</td></tr><tr><td>E. limosum (3)</td><td></td><td>3</td><td></td><td>3</td><td></td><td>3</td></tr></tbody></table>
<table><caption>**Table 1 (continued)**</caption><thead><tr><th>Strains (6309)</th><th>V5 database</th><th>V5 database+ENRIA (confirmed)</th><th>Higher score</th><th>Old database+ENRIA (all MSPs)</th><th>Higher score</th></tr></thead><tbody><tr><td>Eubacterium spp. (1)</td><td>1</td><td>1</td><td></td><td>1</td><td></td></tr><tr><td>E. tenue (1)</td><td>1</td><td>1</td><td></td><td>1</td><td></td></tr><tr><td>Eggerthella lenta (65)</td><td>10</td><td>55</td><td>10</td><td>55</td><td>10</td></tr><tr><td>Eggerthia catenaformis (7)</td><td>7</td><td>7</td><td>3</td><td>7</td><td>5</td></tr><tr><td>Flavonifractor plautii (9)</td><td>1</td><td>8</td><td>9</td><td>6</td><td>9</td></tr><tr><td>Helcococcus spp. (15)</td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>H. kunzii (15)</td><td></td><td>15</td><td>15</td><td>2</td><td>15</td></tr><tr><td>Lachnoanaerobaculum spp. (9)</td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>L. orale (7)</td><td>2</td><td>5</td><td>2</td><td>5</td><td>7</td></tr><tr><td>L. umeaense (2)</td><td>2</td><td>5</td><td>2</td><td>5</td><td>2</td></tr><tr><td>Leptotrichia spp. (3)<sup>a</sup></td><td></td><td>3</td><td>3</td><td></td><td>3</td></tr><tr><td>Megasphaera spp. (1)</td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>M. micronuciformis (1)</td><td></td><td>1</td><td>1</td><td></td><td>1</td></tr><tr><td>Moryella indoligenes (2)</td><td>2</td><td></td><td>1</td><td>2</td><td>1</td></tr><tr><td>Mogibacterium spp. (7)</td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>M. timidum (7)</td><td>7</td><td>7</td><td></td><td>6</td><td>1</td></tr><tr><td>Filifactor spp. (9)</td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>F. alocis (9)</td><td>9</td><td>1</td><td>8</td><td>9</td><td>1</td></tr><tr><td>'Fenollaria massiliensis' (7)</td><td>7</td><td>7</td><td></td><td></td><td>7</td></tr><tr><td>Odoribacter spp. (7)</td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>O. splanchnicus (7)</td><td>7</td><td>4</td><td>3</td><td>3</td><td>7</td></tr></tbody></table>
<table><tbody><tr><td>Olsenella spp. (7)</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>O. uli (6)</td><td></td><td>1</td><td>5</td><td></td><td>6</td><td>5</td><td></td><td>6</td><td>5</td></tr><tr><td>Olsenella spp. (1)</td><td>1</td><td></td><td>1</td><td></td><td></td><td></td><td>1</td><td></td><td>1</td></tr><tr><td>Ruminococcus spp. (4)</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>R. gnavus (4)</td><td></td><td>1</td><td>3</td><td></td><td>4</td><td>1</td><td></td><td>4</td><td>1</td></tr><tr><td>Selenomonas spp. (2)</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>S. artemidis (2)</td><td>2</td><td></td><td></td><td></td><td>2</td><td>2</td><td></td><td>2</td><td>2</td></tr><tr><td>Slackia spp. (31)</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>S. exigua (31)</td><td></td><td></td><td>31</td><td></td><td>31</td><td></td><td></td><td>31</td><td></td></tr><tr><td>Solobacterium moorei (41)</td><td></td><td>4</td><td>37</td><td>1</td><td>40</td><td>32</td><td>1</td><td>40</td><td>32</td></tr><tr><td>Sutterella spp. (4)</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>S. wadsworthensis (4)</td><td></td><td></td><td>4</td><td></td><td>4</td><td></td><td></td><td>4</td><td></td></tr><tr><td>Tissierella spp. (1)</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>T. praeacuta (1)</td><td></td><td></td><td>1</td><td></td><td>1</td><td></td><td></td><td>1</td><td></td></tr><tr><td>Actinomyces spp. (306)</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>A. europaeus (11)</td><td>2</td><td>6</td><td>3</td><td>2</td><td>9</td><td>10</td><td>2</td><td>9</td><td>10</td></tr><tr><td>A. funkei (3)</td><td></td><td>2</td><td>1</td><td>2</td><td>1</td><td></td><td>2</td><td>1</td><td></td></tr><tr><td>A. graeventizii (20)</td><td></td><td>3</td><td>17</td><td>1</td><td>19</td><td>11</td><td>1</td><td>19</td><td>12</td></tr><tr><td>A. israelii (2)</td><td></td><td>2</td><td></td><td>2</td><td></td><td></td><td>2</td><td></td><td></td></tr><tr><td>A. meyeri (5)</td><td></td><td>1</td><td>4</td><td>1</td><td>4</td><td></td><td>1</td><td>4</td><td></td></tr><tr><td>A. naeslundii (7)</td><td></td><td>5</td><td>2</td><td>5</td><td>2</td><td></td><td>5</td><td>2</td><td></td></tr><tr><td>A. neuii (37)</td><td></td><td>5</td><td>32</td><td>5</td><td>32</td><td></td><td>5</td><td>32</td><td>15</td></tr><tr><td>A. odontolyticus (121)</td><td></td><td>49</td><td>72</td><td>49</td><td>72</td><td></td><td>49</td><td>72</td><td></td></tr><tr><td>A. oris (36)</td><td></td><td>7</td><td>29</td><td>7</td><td>29</td><td></td><td>7</td><td>29</td><td></td></tr><tr><td>A. radingae (10)</td><td></td><td>4</td><td>6</td><td>3</td><td>7</td><td>8</td><td>3</td><td>7</td><td>8</td></tr><tr><td>A. turicensis (41)</td><td>2</td><td>10</td><td>29</td><td>10</td><td>31</td><td>28</td><td>10</td><td>31</td><td>28</td></tr><tr><td>A. urogenitalis (13)</td><td></td><td>2</td><td>11</td><td>2</td><td>11</td><td>2</td><td>2</td><td>11</td><td>5</td></tr></tbody></table>
<table><caption>**Table 1 (continued)**</caption><thead><tr><th>Strains (6309)</th><th>V5 database</th><th>V5 database+ENRIA (confirmed)</th><th>Higher score</th><th>Old database+ENRIA (all MSPs)</th><th>Higher score</th></tr></thead><tbody><tr><td><strong>Veillonella spp. (241)</strong></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>V. atypica (69)</td><td>3</td><td>66</td><td>3</td><td>66</td><td>68</td></tr><tr><td>V. montpellieriensis (7)</td><td></td><td>7</td><td></td><td>7</td><td>7</td></tr><tr><td>V. ratti (25)</td><td>2</td><td>17</td><td>2</td><td>17</td><td>22</td></tr><tr><td>Veillonella spp. (140)<sup>b</sup></td><td>140</td><td>140</td><td></td><td>140</td><td></td></tr><tr><td><strong>Blautia spp. (1)</strong></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>B. coccoides (1)</td><td></td><td>1</td><td>1</td><td></td><td>1</td></tr><tr><td><strong>Bacteroides spp. (934)</strong></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>B. caccae (16)</td><td>1</td><td>15</td><td>16</td><td>5</td><td>16</td></tr><tr><td>B. cellulosilyticus (10)</td><td>1</td><td>9</td><td>1</td><td>9</td><td>10</td></tr><tr><td>B. clarus (2)</td><td>2</td><td></td><td></td><td>2</td><td>2</td></tr><tr><td>B. coagulans (11)</td><td>1</td><td>7</td><td>3</td><td>3</td><td>8</td></tr><tr><td>B. eggerthii (1)</td><td>1</td><td></td><td></td><td>1</td><td>1</td></tr><tr><td>B. finegoldii (2)</td><td></td><td>2</td><td></td><td>2</td><td>2</td></tr><tr><td>B. fragilis (504)</td><td>5</td><td>499</td><td>5</td><td>499</td><td>499</td></tr><tr><td>B. intestinalis (2)</td><td>1</td><td>1</td><td></td><td>2</td><td>2</td></tr><tr><td>B. massiliensis (3)</td><td>2</td><td>1</td><td></td><td>3</td><td>3</td></tr><tr><td>B. ovatus/xylanisolvens (85)</td><td>2</td><td>16</td><td>2</td><td>16</td><td>75</td></tr><tr><td>B. plebeius (1)</td><td>1</td><td></td><td>1</td><td></td><td></td></tr><tr><td>B. pyogenes (8)</td><td></td><td>8</td><td></td><td>8</td><td>8</td></tr><tr><td>B. salyersiae (10)</td><td></td><td>10</td><td></td><td>7</td><td>10</td></tr><tr><td>B. thetaiotaomicron/faecis (140)</td><td>4</td><td>136</td><td>3</td><td>137</td><td>137</td></tr><tr><td>B. uniformis (38)</td><td>1</td><td>37</td><td>1</td><td>37</td><td>37</td></tr><tr><td>B. vulgatus/dorei (91)</td><td>2</td><td>89</td><td>2</td><td>89</td><td>90</td></tr><tr><td>B. nordii (5)</td><td>2</td><td>3</td><td></td><td>5</td><td>5</td></tr><tr><td>B. stercoris (5)</td><td>1</td><td>4</td><td>1</td><td>4</td><td>5</td></tr><tr><td><strong>Clostridium spp. (225)</strong></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>C. aldenense (5)</td><td></td><td>5</td><td></td><td>5</td><td>5</td></tr><tr><td>C. baratii (4)</td><td></td><td>4</td><td></td><td>4</td><td>4</td></tr><tr><td>C. bolteae (1)</td><td></td><td>1</td><td></td><td>1</td><td>1</td></tr><tr><td>C. butyricum (11)</td><td></td><td>11</td><td></td><td>11</td><td>11</td></tr><tr><td>C. cadaveris (1)</td><td></td><td>1</td><td></td><td>1</td><td>1</td></tr><tr><td>C. citronae (7)</td><td>3</td><td>4</td><td>2</td><td>5</td><td>5</td></tr><tr><td>C. clostridioforme (23)</td><td>1</td><td>22</td><td>1</td><td>22</td><td>22</td></tr><tr><td>C. colicaris (1)</td><td>1</td><td></td><td>1</td><td></td><td>1</td></tr><tr><td>C. indolis (3)</td><td></td><td>3</td><td></td><td>3</td><td>3</td></tr><tr><td>C. innocuum (25)</td><td>12</td><td>13</td><td>12</td><td>13</td><td>13</td></tr></tbody></table>
<table><tbody><tr><td>C. paraputrificum (7)</td><td></td><td>7</td><td></td><td>7</td><td></td><td>7</td><td></td></tr><tr><td>C. perfringens (65)</td><td>5</td><td>60</td><td>5</td><td>60</td><td>4</td><td>61</td><td>2</td></tr><tr><td>C. ramosum (35)</td><td>3</td><td>32</td><td>3</td><td>32</td><td>3</td><td>32</td><td></td></tr><tr><td>C. sardiniense (1)</td><td></td><td>1</td><td></td><td>1</td><td></td><td>1</td><td></td></tr><tr><td>C. scindens (1)</td><td></td><td>1</td><td></td><td>1</td><td></td><td>1</td><td></td></tr><tr><td>C. septicum (2)</td><td></td><td>2</td><td></td><td>2</td><td></td><td>2</td><td></td></tr><tr><td>C. sphenoides (6)</td><td></td><td>6</td><td></td><td>6</td><td></td><td>6</td><td></td></tr><tr><td>C. sporogenes (7)</td><td></td><td>7</td><td></td><td>7</td><td></td><td>7</td><td></td></tr><tr><td>C. symbiosum (6)</td><td>2</td><td>4</td><td></td><td>6</td><td>6</td><td>6</td><td>6</td></tr><tr><td>C. tertium (10)</td><td>2</td><td>8</td><td>2</td><td>8</td><td>2</td><td>8</td><td></td></tr><tr><td>C. celatum (2)</td><td></td><td>2</td><td></td><td>2</td><td></td><td>2</td><td>2</td></tr><tr><td>Clostridium spp. (2)</td><td>2</td><td></td><td>2</td><td></td><td>2</td><td></td><td></td></tr><tr><td>Paraclostridium spp. (5)</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>P. bifermentans (5)</td><td>4</td><td>1</td><td>4</td><td>1</td><td>4</td><td>1</td><td></td></tr><tr><td>Clostridioides spp. (413)</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>C. difficile (413)</td><td>17</td><td>396</td><td>17</td><td>396</td><td>17</td><td>396</td><td></td></tr><tr><td>Hungatella spp. (16)</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>H. hathewayi (16)</td><td></td><td>16</td><td></td><td>16</td><td></td><td>16</td><td>5</td></tr><tr><td>Terrisporobacter spp. (2)</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>T. glycolicus (2)</td><td></td><td>2</td><td></td><td>2</td><td></td><td>2</td><td>1</td></tr><tr><td>Paeniclostridium spp. (10)</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>P. sordellii (10)</td><td>1</td><td>9</td><td>1</td><td>9</td><td>1</td><td>9</td><td>3</td></tr><tr><td>Intestinibacter spp. (1)</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>I. bartletii (1)</td><td>1</td><td></td><td>1</td><td></td><td>1</td><td></td><td></td></tr><tr><td>Hathewaya spp. (2)</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>H. histolytica (2)</td><td></td><td>2</td><td></td><td>2</td><td></td><td>2</td><td></td></tr></tbody></table>
<table><caption>**Table 1 (continued)**</caption><thead><tr><th>Strains (6309)</th><th>V5 database</th><th>V5 database+ENRIA (confirmed)</th><th>Higher score</th><th>Old database+ENRIA (all MSPs)</th><th>Higher score</th></tr></thead><tbody><tr><td><strong>Parabacteroides</strong> spp. (54)</td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>P. distasonis (45)</td><td>1</td><td>44</td><td>1</td><td>44</td><td>24</td></tr><tr><td>P. goldsteinii (3)</td><td></td><td>3</td><td></td><td>3</td><td>3</td></tr><tr><td>P. johnsonii (1)</td><td></td><td>1</td><td></td><td>1</td><td>1</td></tr><tr><td>P. merdae (5)</td><td>1</td><td>4</td><td></td><td>5</td><td>5</td></tr><tr><td><strong>Prevotella</strong> spp. (582)</td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>P. amnii (2)</td><td></td><td>2</td><td></td><td>2</td><td>2</td></tr><tr><td>P. baroniae (18)</td><td>1</td><td>16</td><td>2</td><td>16</td><td>13</td></tr><tr><td>P. bergensis (22)</td><td>3</td><td>19</td><td>2</td><td>20</td><td>17</td></tr><tr><td>P. bivia (112)</td><td>8</td><td>104</td><td>8</td><td>104</td><td>5</td></tr><tr><td>P. buccae (64)</td><td>5</td><td>59</td><td>5</td><td>59</td><td>5</td></tr><tr><td>P. buccalis (15)</td><td>7</td><td>1</td><td>4</td><td>11</td><td>14</td></tr><tr><td>P. copri (2)</td><td></td><td>2</td><td></td><td>2</td><td>2</td></tr><tr><td>P. corporis (14)</td><td>3</td><td>11</td><td>1</td><td>13</td><td>9</td></tr><tr><td>P. dentalis (5)</td><td></td><td>5</td><td></td><td>5</td><td>5</td></tr><tr><td>P. denticola (39)</td><td></td><td>39</td><td></td><td>39</td><td>39</td></tr><tr><td>P. disiens (25)</td><td>3</td><td>22</td><td>3</td><td>22</td><td>2</td></tr><tr><td>P. histicola (9)</td><td>1</td><td>8</td><td>1</td><td>8</td><td>5</td></tr><tr><td>P. intermedia (27)</td><td>1</td><td>5</td><td>1</td><td>4</td><td>6</td></tr><tr><td>P. jejuni (5)</td><td>4</td><td>1</td><td>4</td><td>1</td><td>4</td></tr><tr><td>P. loeschii (1)</td><td></td><td>1</td><td></td><td>1</td><td>1</td></tr><tr><td>P. maculosa (2)</td><td></td><td>2</td><td></td><td>2</td><td>2</td></tr><tr><td>'P. massiliensis' (2)</td><td>2</td><td></td><td>2</td><td></td><td>2</td></tr><tr><td>P. melaninogenica (64)</td><td>5</td><td>15</td><td>44</td><td>15</td><td>50</td></tr><tr><td>P. heparinolytica (13)</td><td></td><td>13</td><td></td><td>13</td><td>13</td></tr><tr><td>P. nanceiensis (14)</td><td></td><td>12</td><td></td><td>12</td><td>12</td></tr><tr><td>P. nigrescens (48)</td><td>1</td><td>7</td><td>40</td><td>7</td><td>40</td></tr><tr><td>P. oris (13)</td><td></td><td>13</td><td></td><td>13</td><td>13</td></tr><tr><td>P. pallens (1)</td><td></td><td>1</td><td></td><td>1</td><td>1</td></tr><tr><td>P. oulorum (3)</td><td></td><td>2</td><td></td><td>2</td><td>2</td></tr><tr><td>P. salivae (11)</td><td>6</td><td>5</td><td>1</td><td>11</td><td>11</td></tr><tr><td>P. timonensis (42)</td><td>2</td><td>9</td><td>31</td><td>1</td><td>40</td></tr><tr><td>P. veroralis (2)</td><td></td><td>1</td><td></td><td>2</td><td>2</td></tr><tr><td>P. oralis (3)</td><td>1</td><td>2</td><td></td><td>3</td><td>3</td></tr><tr><td>P. veroralis (1)</td><td></td><td>1</td><td></td><td>1</td><td>1</td></tr><tr><td><strong>Prevotella</strong> spp. (3)</td><td></td><td>3</td><td></td><td>3</td><td>3</td></tr><tr><td><strong>Fusobacterium</strong> spp. (303)</td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>F. canifelinum (1)</td><td></td><td>1</td><td></td><td>1</td><td></td></tr><tr><td>F. gonidiaformans (16)</td><td></td><td>16</td><td></td><td>16</td><td>16</td></tr></tbody></table>
<table><tr><td>F. necrophorum (52)</td><td>2</td><td>50</td><td>1</td><td>51</td><td>16</td><td>1</td><td>51</td><td>18</td></tr><tr><td>F. nucleatum (200)</td><td>6</td><td>60</td><td>134</td><td>6</td><td>60</td><td>134</td><td>47</td><td>153</td><td>82</td></tr><tr><td>F. periodonticum (14)</td><td>13</td><td>1</td><td>13</td><td>1</td><td>1</td><td>13</td><td>1</td><td>1</td></tr><tr><td>F. ulcerans (5)</td><td></td><td>5</td><td></td><td>5</td><td>2</td><td></td><td>5</td><td>2</td></tr><tr><td>F. varium (3)</td><td></td><td>3</td><td></td><td>3</td><td></td><td></td><td>3</td><td>1</td></tr><tr><td>Fusobacterium spp. (12)</td><td>1</td><td>4</td><td>7</td><td>1</td><td>4</td><td>7</td><td>5</td><td>7</td><td>1</td></tr></table>

<table><tr><td colspan="9">Anaerococcus spp. (230)</td></tr><tr><td>A. hydrogenalis (12)</td><td>4</td><td>8</td><td>4</td><td>8</td><td>4</td><td>8</td><td></td><td></td></tr><tr><td>A. lactolyticus (11)</td><td>5</td><td>6</td><td>1</td><td>10</td><td>10</td><td>1</td><td>10</td><td>10</td></tr><tr><td>A. murdochii (34)</td><td>2</td><td>4</td><td>28</td><td>1</td><td>4</td><td>18</td><td>4</td><td>30</td><td>24</td></tr><tr><td>A. degeneri (8)</td><td>5</td><td>3</td><td>5</td><td>3</td><td>5</td><td>1</td><td>7</td><td>8</td></tr><tr><td>A. octavius (6)</td><td>1</td><td>5</td><td>1</td><td>5</td><td>1</td><td>2</td><td>6</td><td>1</td></tr><tr><td>A. prevotii (3)</td><td>2</td><td>1</td><td>2</td><td>2</td><td>2</td><td>2</td><td>1</td><td></td></tr><tr><td>A. tet radius (7)</td><td>5</td><td>2</td><td>5</td><td>2</td><td>5</td><td>5</td><td>2</td><td></td></tr><tr><td>A. vaginalis (107)</td><td>30</td><td>64</td><td>13</td><td>11</td><td>37</td><td>59</td><td>16</td><td>91</td><td>107</td></tr><tr><td>Anaerococcus spp. (28)</td><td>1</td><td>4</td><td>23</td><td>1</td><td>4</td><td>23</td><td>5</td><td>23</td><td>1</td></tr><tr><td>A. senegalensis (10)</td><td>9</td><td>1</td><td>9</td><td>1</td><td>1</td><td></td><td>10</td><td>10</td></tr><tr><td>A. nagyae (4)</td><td>4</td><td></td><td>4</td><td></td><td></td><td>1</td><td>3</td><td>4</td></tr></table>

<table><tr><td>Finegoldia magna (412)</td><td>87</td><td>325</td><td>87</td><td>325</td><td>87</td><td>325</td><td></td><td></td></tr></table>

<table><tr><td>Murdochiella asaccharolytica (13)</td><td>5</td><td>8</td><td>4</td><td>9</td><td>6</td><td>4</td><td>9</td><td>6</td></tr></table>

<table><tr><td colspan="9">Peptoniphilus spp. (349)</td></tr><tr><td>P. duerdenii (7)</td><td>7</td><td></td><td>7</td><td></td><td></td><td></td><td>7</td><td>7</td></tr><tr><td>P. olsenii (8)</td><td>8</td><td></td><td></td><td>8</td><td>8</td><td></td><td>8</td><td>8</td></tr><tr><td>P. tyrrelliae (4)</td><td>4</td><td></td><td></td><td>4</td><td>4</td><td></td><td>4</td><td>4</td></tr><tr><td>P. rhinitidis (8)</td><td>8</td><td></td><td>8</td><td></td><td></td><td></td><td>8</td><td>8</td></tr><tr><td>P. koenoeneniae (1)</td><td>1</td><td></td><td></td><td>1</td><td>1</td><td></td><td>1</td><td>1</td></tr><tr><td>P. lacrimalis (20)</td><td>20</td><td></td><td>1</td><td>1</td><td>18</td><td>19</td><td>19</td><td>20</td></tr><tr><td>P. gorbachii (12)</td><td>1</td><td>1</td><td>10</td><td>1</td><td>11</td><td>10</td><td>1</td><td>12</td></tr><tr><td>'P. grossensis' (18)</td><td>13</td><td>5</td><td>13</td><td>5</td><td>200</td><td>26</td><td>20</td><td>18</td></tr><tr><td>P. harei (241)</td><td>4</td><td>41</td><td>196</td><td>2</td><td>39</td><td></td><td></td><td>221</td><td>192</td></tr><tr><td>P. ivorii (1)</td><td></td><td></td><td>1</td><td></td><td>1</td><td></td><td>1</td><td>1</td></tr><tr><td>P. coxii (27)</td><td></td><td>10</td><td>17</td><td></td><td>5</td><td>17</td><td></td><td>27</td><td>27</td></tr><tr><td>P. asaccharolyticus (2)</td><td></td><td>2</td><td></td><td>2</td><td></td><td>2</td><td></td><td></td></tr></table>
## 2. Experimental design, materials and methods

### 2.1. Bacterial strains

The expertise laboratories:

- University Medical Center Groningen (UMCG), Groningen, The Netherlands;

- Centre Hospitalier Universitaire de Montpellier, Montpellier, France;

- Odense University Hospital, Odense, Denmark;

- UK Anaerobe Reference Unit (UKARU), Public Health Wales Microbiology, Cardiff, United Kingdom;

- University of Szeged, Szeged, Hungary and

- Universitair Ziekenhuis Brussel, Brussels, Belgium.

All utilized 6 months' worth of anaerobic human clinical isolates encountered and identified using the MALDI-TOF MS Biotyper system (Bruker Daltonics, Bremen, Germany), which resulted in a total of 6309 isolates used for validation. The obtained spectra were compared with the V5 database, the V5 database plus the ENRIA MSPs which were added to the next update of the database and the V5 database plus all MSPs created from the collected ENRIA strains. All MSPs were created and supplied by Bruker Daltonics.

### 2.2. Identification

The MALDI-TOF MS measurements were performed at each laboratory as described previously [2]. The measurements were performed as part of the daily routine, using standard settings. Obtained log scores were interpreted as advised by the manufacturer.

### 2.3. Data interpretation

The identifications obtained were divided into 3 groups.

- Group 1 (log score < 1.7)=reliable identification.

- Group 2 (log score ≥ 1.7 and < 2)=identification with low confidence e.g. reliable genus only.

- Group 3 (log score ≥ 2)=identification with high confidence e.g. reliable species.

Identifications to the subspecies level were not considered during the data analyses.

Species that cannot be differentiated from each other using MALDI-TOF MS were presented as such: e.g. *Bacteroides ovatus/xylanisolvens*, *Bacteroides thetaiotaomicron/faecis*, *Bacteroides vulgatus/dorei* and *Fusobacterium nucleatum/naviforme*.

Species that cannot be reliably identified at the species level using 16S rRNA sequencing were assumed to be either: e.g. *Porphyromonas asaccharolytica/uenonis*. This included strains identified as *Veillonella dispar*, *Veillonella parvula*, *Veillonella denticariosi* and *Veillonella rogosae*. These strains were categorized as being *Veillonella* species, regardless of the obtained log score. No differentiation was made between valid and non-valid species.

## Acknowledgements

by InterregIVa ENRIA is partly funded by InterregIVa (III-1-02=73), for the identification of neglected infectious disease and within the task of reference laboratory in the Dutch-German border region. No direct funding was received from Bruker Daltonics. However, Bruker Daltonics provided for this study knowledge, expertise and is an equal partner in ENRIA.
**Transparency document. Supporting information**

Supplementary data associated with this article can be found in the online version at http://dx.doi.org/10.1016/j.dib.2018.04.070.

**References**

[1] A.C.M. Veloo, H. Jean-Pierre, U.S. Justesen, T. Morris, E. Urban, I. Wybo, M. Kostrzewa, A.W. Friedrich, on behalf of the ENRIA workgroup, Validation of a for anaerobic bacteria optimized MALDI-TOF MS Biotyper database: the ENRIA project, Anaerobe (2018), http://dx.doi.org/10.1016/j.anaerobe.2018.03.007.

[2] A.C.M. Veloo, P.E. Elgersma, A.W. Friedrich, E. Nagy, A.J. van Winkelhoff, The influence of incubation time, sample preparation and exposure to oxygen on the quality of the MALDI-TOF MS spectrum of anaerobic bacteria, Clin. Microbiol Infect. 20 (2014) 1091–1097.