TCR signaling and cellular metabolism regulate the
capacity of murine epidermal $\gamma\delta$ T cells to rapidly
produce IL-13 but not IFN-$\gamma$

Atsuko Ibusuki, Kazuhiro Kawai, Ayano Nitahara-Takeuchi, Rafael J

Argüello, Takuro Kanekura

► To cite this version:

Atsuko Ibusuki, Kazuhiro Kawai, Ayano Nitahara-Takeuchi, Rafael J Argüello, Takuro Kanekura. TCR signaling and cellular metabolism regulate the capacity of murine epidermal $\gamma\delta$ T cells to rapidly produce IL-13 but not IFN-$\gamma$. Frontiers in Immunology, 2024, 15, 10.3389/fimmu.2024.1361139. hal-04542863

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EDITED BY

Akihiko Yoshimura,
Keio University, Japan

REVIEWED BY

Hiroko Nakatsukasa,
Chiba University, Japan
Shigenori Nagai,
Tokyo Medical and Dental University, Japan

*CORRESPONDENCE

Kazuhiro Kawai

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kazkawai@m2.kufm.kagoshima-u.ac.jp

RECEIVED 25 December 2023

ACCEPTED 08 February 2024

PUBLISHED 28 February 2024

CITATION

Ibusuki A, Kawai K, Nitahara-Takeuchi A, Argüello RJ and Kanekura T (2024) TCR signaling and cellular metabolism regulate the capacity of murine epidermal γδ T cells to rapidly produce IL-13 but not IFN-γ.
*Front. Immunol.* **15**:1361139.
doi: **10.3389/fimmu.2024.1361139**

COPYRIGHT

© 2024 Ibusuki, Kawai, Nitahara-Takeuchi, Argüello and Kanekura. This is an open-access article distributed under the terms of the **Creative Commons Attribution License (CC BY)**. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

# TCR signaling and cellular
metabolism regulate the
capacity of murine epidermal
γδ T cells to rapidly produce
IL-13 but not IFN-γ

Atsuko Ibusuki<fnref n="1" />, Kazuhiro Kawai<fnref n="1" />,²*, Ayano Nitahara-Takeuchi³,
Rafael J. Argüello<fnref n="4" /> and Takuro Kanekura<fnref n="1" />

Resident epidermal T cells of murine skin, called dendritic epidermal T cells (DETCs), express an invariant γδ TCR that recognizes an unidentified self-ligand expressed on epidermal keratinocytes. Although their fetal thymic precursors are preprogrammed to produce IFN-γ, DETCs in the adult epidermis rapidly produce IL-13 but not IFN-γ early after activation. Here, we show that preprogrammed IFN-γ-producing DETC precursors differentiate into rapid IL-13 producers in the perinatal epidermis. The addition of various inhibitors of signaling pathways downstream of TCR to the *in vitro* differentiation model of neonatal DETCs revealed that TCR signaling through the p38 MAPK pathway is essential for the functional differentiation of neonatal DETCs. Constitutive TCR signaling at steady state was also shown to be needed for the maintenance of the rapid IL-13-producing capacity of adult DETCs because *in vivo* treatment with the p38 MAPK inhibitor decreased adult DETCs with the rapid IL-13-producing capacity. Adult DETCs under steady-state conditions had lower glycolytic capacity than proliferating neonatal DETCs. TCR stimulation of adult DETCs induced high glycolytic capacity and IFN-γ production during the late phase of activation. Inhibition of glycolysis decreased IFN-γ but not IL-13 production by adult DETCs during the late phase of activation. These results demonstrate that TCR signaling promotes the differentiation of IL-13-producing DETCs in the perinatal epidermis and is needed for maintaining the rapid IL-13-producing capacity of adult DETCs. The low glycolytic capacity of adult DETCs at steady state also regulates the rapid IL-13 response and delayed IFN-γ production after activation.

KEYWORDS

mouse, intraepithelial lymphocytes, cytokine, T-cell receptor, mTORC1

<footnote n="1">¹Department of Dermatology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan, ²Department of Dermatology, Kido Hospital, Niigata, Japan, ³Division of Dermatology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan,</footnote>

<footnote n="4">⁴Aix Marseille Université, CNRS, INSERM, CIML, Centre d'Immunologie de Marseille-Luminy, Marseille, France</footnote>
<figure><img src="image_2.png" /></figure>

Check for updates

OPEN ACCESS

EDITED BY

Akihiko Yoshimura,
Keio University, Japan

REVIEWED BY

Hiroko Nakatsukasa,
Chiba University, Japan
Shigenori Nagai,
Tokyo Medical and Dental University, Japan

*CORRESPONDENCE

Kazuhiro Kawai

<figure><img src="image_3.png" /></figure>

kazkawai@m2.kufm.kagoshima-u.ac.jp

RECEIVED 25 December 2023

ACCEPTED 08 February 2024

PUBLISHED 28 February 2024

CITATION

Ibusuki A, Kawai K, Nitahara-Takeuchi A, Argüello RJ and Kanekura T (2024) TCR signaling and cellular metabolism regulate the capacity of murine epidermal γδ T cells to rapidly produce IL-13 but not IFN-γ.
*Front. Immunol.* **15**:1361139.
doi: **10.3389/fimmu.2024.1361139**

COPYRIGHT

© 2024 Ibusuki, Kawai, Nitahara-Takeuchi, Argüello and Kanekura. This is an open-access article distributed under the terms of the **Creative Commons Attribution License (CC BY)**. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

# TCR signaling and cellular
metabolism regulate the
capacity of murine epidermal
γδ T cells to rapidly produce
IL-13 but not IFN-γ

Atsuko Ibusuki¹, Kazuhiro Kawai¹,²*, Ayano Nitahara-Takeuchi³,
Rafael J. Argüello⁴ and Takuro Kanekura¹

¹Department of Dermatology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan, ²Department of Dermatology, Kido Hospital, Niigata, Japan, ³Division of Dermatology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan, ⁴Aix Marseille Université, CNRS, INSERM, CIML, Centre d'Immunologie de Marseille-Luminy, Marseille, France

Resident epidermal T cells of murine skin, called dendritic epidermal T cells (DETCs), express an invariant γδ TCR that recognizes an unidentified self-ligand expressed on epidermal keratinocytes. Although their fetal thymic precursors are preprogrammed to produce IFN-γ, DETCs in the adult epidermis rapidly produce IL-13 but not IFN-γ early after activation. Here, we show that preprogrammed IFN-γ-producing DETC precursors differentiate into rapid IL-13 producers in the perinatal epidermis. The addition of various inhibitors of signaling pathways downstream of TCR to the *in vitro* differentiation model of neonatal DETCs revealed that TCR signaling through the p38 MAPK pathway is essential for the functional differentiation of neonatal DETCs. Constitutive TCR signaling at steady state was also shown to be needed for the maintenance of the rapid IL-13-producing capacity of adult DETCs because *in vivo* treatment with the p38 MAPK inhibitor decreased adult DETCs with the rapid IL-13-producing capacity. Adult DETCs under steady-state conditions had lower glycolytic capacity than proliferating neonatal DETCs. TCR stimulation of adult DETCs induced high glycolytic capacity and IFN-γ production during the late phase of activation. Inhibition of glycolysis decreased IFN-γ but not IL-13 production by adult DETCs during the late phase of activation. These results demonstrate that TCR signaling promotes the differentiation of IL-13-producing DETCs in the perinatal epidermis and is needed for maintaining the rapid IL-13-producing capacity of adult DETCs. The low glycolytic capacity of adult DETCs at steady state also regulates the rapid IL-13 response and delayed IFN-γ production after activation.

KEYWORDS

mouse, intraepithelial lymphocytes, cytokine, T-cell receptor, mTORC1
Introduction

γδ T cells represent a minor population of T cells in adult blood and peripheral lymphoid organs but are enriched in epithelial tissues (1, 2). In mice, different epithelial tissues are populated by distinct γδ T-cell subsets defined by the usage of specific TCR Vγ regions (3). These epithelial γδ T-cell subsets develop in waves based on ordered Vγ gene rearrangement in the fetal and neonatal thymus and are home to specific tissues. Epithelial γδ T cells mediate stress surveillance and exert a distinct set of effector functions in a given tissue (4–8).

Unlike conventional αβ T cells, which differentiate into effector subsets during activation in peripheral lymphoid organs (9), most epithelial γδ T cells are preprogrammed during thymic development to either IFN-γ- or IL-17A-producing effector subsets that exhibit rapid, innate-like responses in the periphery (10–12). Recent studies have revealed the role of TCR signaling in the differentiation of effector subsets during thymic development. Ligand-induced strong TCR signaling is needed for the differentiation of IFN-γ-producing γδ T cells, whereas weaker TCR signaling supports the differentiation of IL-17A-producing cells (13–18). IFN-γ-producing γδ T-cell development also requires CD27 signaling, and mature IFN-γ-producing γδ T cells usually express CD27, while IL-17A-producing γδ T cells lack CD27 expression (14).

Resident epidermal T cells of murine skin, called dendritic epidermal T cells (DETCs), are prototypic epithelial γδ T cells that express an invariant Vγ3Vδ1 TCR (Garman nomenclature) (19). DETCs contribute to epidermal homeostasis, wound healing, IgE production, and tumor surveillance (7, 20–25). Although the ligand of the Vγ3Vδ1 TCR has not been identified, it is expressed on fetal thymic epithelial cells and stressed or transformed epidermal keratinocytes (26–29). Accumulating evidence suggests that low levels of the TCR ligand are constitutively expressed on keratinocytes at steady state (20, 30). DETC precursors develop as the first T cells in the fetal thymus. Thymic maturation of DETC precursors requires ligand-dependent TCR signaling and the thymic stromal determinants Skint1 and Skint2 (15, 31, 32). TCR signaling in DETC precursors in the fetal thymus promotes the upregulation of skin homing receptors needed for thymic export and skin homing (33–35), and DETC precursors migrate to the skin before birth. After seeding the epidermis in low numbers, DETCs massively proliferate until 2 to 6 weeks after birth, depending on the mouse strain (36, 37), and their numbers become stable by 8 weeks (27).

Ligand-selected mature Vγ3+ fetal thymocytes that have received strong TCR signaling express CD27 and Tbx21, which encodes T-bet, the master transcription factor of IFN-γ-producing cells, and rapidly produce IFN-γ following phorbol 12-myristate 13-acetate (PMA) and ionomycin stimulation (15). In contrast, DETCs in the adult epidermis do not express CD27 (38, 39) or produce IFN-γ within 4 hours after stimulation with PMA/ionomycin (22, 40) but do begin to produce IFN-γ 12 to 24 hours after stimulation in vitro (39, 41). This could be due to the hyporesponsive TCR signaling that occurs during DETC development (42). However,

adult DETCs produce IL-13 upon short-term PMA/ionomycin stimulation (22). Therefore, Vγ3+ T cells appear to differentiate from preprogrammed IFN-γ producers into rapid IL-13 producers after thymic egress. This functional switch in DETCs sets them apart from other tissue-resident γδ T cells preprogrammed to produce IFN-γ, and the distinct kinetics of IL-13 and IFN-γ production by DETCs is important for DETC-mediated stress surveillance in the epidermis (22, 24). However, it remains unknown when, where, and how the functional switch of Vγ3+ T cells occurs.

We aimed to clarify the timing, location, and underlying mechanisms of the functional switch of Vγ3+ T cells. Here, we show that Vγ3+ T cells differentiate from preprogrammed IFN-γ producers into rapid IL-13 producers in the perinatal epidermis and that this differentiation is dependent on TCR signaling through the p38 mitogen-activated protein kinase (MAPK) pathway. We also show that the rapid IL-13-producing capacity of adult DETCs under steady-state conditions is maintained by continuous TCR signaling and cellular metabolism.

Results

Adult DETCs predominantly produce IL-13
during the early phase of activation

Adult DETCs produce IL-13 but not IFN-γ upon short-term PMA/ionomycin stimulation (22). To quantify cytokine levels secreted by adult DETCs upon TCR stimulation, we purified DETCs from adult ear epidermal cells without TCR ligation by positive magnetic selection using an anti-integrin β7 mAb. The purified DETCs were >95% pure Vγ3+ T cells and contained <1% non-Vγ3+ T cells and <1% IA+ Langerhans cells (Figure 1A). Although DETCs were reported to constitutively produce IL-13 at steady state (22), purified DETCs cultured without TCR stimulation did not secrete IL-13 or other cytokines (Figure 1B). The purified DETCs predominantly secreted IL-13 during the first 24 hours of TCR stimulation, and IFN-γ secretion increased after 24 hours. Consistent with a previous study (39), DETCs secreted a small amount of IL-17A upon TCR stimulation, but IL-4 secretion was not detected (Figure 1B).

RT-PCR analysis revealed that purified naive DETCs constitutively expressed Gata3, the master transcription factor of type 2 cytokine-producing cells, but Tbx21 was expressed only after activation (Figure 1C). Eomes, which encodes eomesodermin that also regulates IFN-γ production in type 1 cytokine-producing T cells, was not expressed in DETCs even after activation (Figure 1C). Consistent with the IL-17A-producing capacity of a subpopulation of DETCs, the expression of Rorc, which encodes RORγt, the master transcription factor of IL-17A-producing cells, was detected in naive DETCs (Figure 1C). The lack of IL-4 production by DETCs might be explained by the constitutive expression of Zbtb32 (Figure 1C), which encodes ZBTB32 (Repressor of GATA, ROG) that represses Il4 but not Il13 gene activation in type 2 CD8+ cytotoxic T lymphocytes (43).
Introduction

γδ T cells represent a minor population of T cells in adult blood and peripheral lymphoid organs but are enriched in epithelial tissues (1, 2). In mice, different epithelial tissues are populated by distinct γδ T-cell subsets defined by the usage of specific TCR Vγ regions (3). These epithelial γδ T-cell subsets develop in waves based on ordered Vγ gene rearrangement in the fetal and neonatal thymus and are home to specific tissues. Epithelial γδ T cells mediate stress surveillance and exert a distinct set of effector functions in a given tissue (4–8).

Unlike conventional αβ T cells, which differentiate into effector subsets during activation in peripheral lymphoid organs (9), most epithelial γδ T cells are preprogrammed during thymic development to either IFN-γ- or IL-17A-producing effector subsets that exhibit rapid, innate-like responses in the periphery (10–12). Recent studies have revealed the role of TCR signaling in the differentiation of effector subsets during thymic development. Ligand-induced strong TCR signaling is needed for the differentiation of IFN-γ-producing γδ T cells, whereas weaker TCR signaling supports the differentiation of IL-17A-producing cells (13–18). IFN-γ-producing γδ T-cell development also requires CD27 signaling, and mature IFN-γ-producing γδ T cells usually express CD27, while IL-17A-producing γδ T cells lack CD27 expression (14).

Resident epidermal T cells of murine skin, called dendritic epidermal T cells (DETCs), are prototypic epithelial γδ T cells that express an invariant Vγ3Vδ1 TCR (Garman nomenclature) (19). DETCs contribute to epidermal homeostasis, wound healing, IgE production, and tumor surveillance (7, 20–25). Although the ligand of the Vγ3Vδ1 TCR has not been identified, it is expressed on fetal thymic epithelial cells and stressed or transformed epidermal keratinocytes (26–29). Accumulating evidence suggests that low levels of the TCR ligand are constitutively expressed on keratinocytes at steady state (20, 30). DETC precursors develop as the first T cells in the fetal thymus. Thymic maturation of DETC precursors requires ligand-dependent TCR signaling and the thymic stromal determinants Skint1 and Skint2 (15, 31, 32). TCR signaling in DETC precursors in the fetal thymus promotes the upregulation of skin homing receptors needed for thymic export and skin homing (33–35), and DETC precursors migrate to the skin before birth. After seeding the epidermis in low numbers, DETCs massively proliferate until 2 to 6 weeks after birth, depending on the mouse strain (36, 37), and their numbers become stable by 8 weeks (27).

Ligand-selected mature Vγ3+ fetal thymocytes that have received strong TCR signaling express CD27 and Tbx21, which encodes T-bet, the master transcription factor of IFN-γ-producing cells, and rapidly produce IFN-γ following phorbol 12-myristate 13-acetate (PMA) and ionomycin stimulation (15). In contrast, DETCs in the adult epidermis do not express CD27 (38, 39) or produce IFN-γ within 4 hours after stimulation with PMA/ionomycin (22, 40) but do begin to produce IFN-γ 12 to 24 hours after stimulation in vitro (39, 41). This could be due to the hyporesponsive TCR signaling that occurs during DETC development (42). However,

adult DETCs produce IL-13 upon short-term PMA/ionomycin stimulation (22). Therefore, Vγ3+ T cells appear to differentiate from preprogrammed IFN-γ producers into rapid IL-13 producers after thymic egress. This functional switch in DETCs sets them apart from other tissue-resident γδ T cells preprogrammed to produce IFN-γ, and the distinct kinetics of IL-13 and IFN-γ production by DETCs is important for DETC-mediated stress surveillance in the epidermis (22, 24). However, it remains unknown when, where, and how the functional switch of Vγ3+ T cells occurs.

We aimed to clarify the timing, location, and underlying mechanisms of the functional switch of Vγ3+ T cells. Here, we show that Vγ3+ T cells differentiate from preprogrammed IFN-γ producers into rapid IL-13 producers in the perinatal epidermis and that this differentiation is dependent on TCR signaling through the p38 mitogen-activated protein kinase (MAPK) pathway. We also show that the rapid IL-13-producing capacity of adult DETCs under steady-state conditions is maintained by continuous TCR signaling and cellular metabolism.

Results

Adult DETCs predominantly produce IL-13
during the early phase of activation

Adult DETCs produce IL-13 but not IFN-γ upon short-term PMA/ionomycin stimulation (22). To quantify cytokine levels secreted by adult DETCs upon TCR stimulation, we purified DETCs from adult ear epidermal cells without TCR ligation by positive magnetic selection using an anti-integrin β7 mAb. The purified DETCs were >95% pure Vγ3+ T cells and contained <1% non-Vγ3+ T cells and <1% IA+ Langerhans cells (Figure 1A). Although DETCs were reported to constitutively produce IL-13 at steady state (22), purified DETCs cultured without TCR stimulation did not secrete IL-13 or other cytokines (Figure 1B). The purified DETCs predominantly secreted IL-13 during the first 24 hours of TCR stimulation, and IFN-γ secretion increased after 24 hours. Consistent with a previous study (39), DETCs secreted a small amount of IL-17A upon TCR stimulation, but IL-4 secretion was not detected (Figure 1B).

RT-PCR analysis revealed that purified naive DETCs constitutively expressed Gata3, the master transcription factor of type 2 cytokine-producing cells, but Tbx21 was expressed only after activation (Figure 1C). Eomes, which encodes eomesodermin that also regulates IFN-γ production in type 1 cytokine-producing T cells, was not expressed in DETCs even after activation (Figure 1C). Consistent with the IL-17A-producing capacity of a subpopulation of DETCs, the expression of Rorc, which encodes RORγt, the master transcription factor of IL-17A-producing cells, was detected in naive DETCs (Figure 1C). The lack of IL-4 production by DETCs might be explained by the constitutive expression of Zbtb32 (Figure 1C), which encodes ZBTB32 (Repressor of GATA, ROG) that represses Il4 but not Il13 gene activation in type 2 CD8+ cytotoxic T lymphocytes (43).
A Crude epidermal cells Purified DETCs

<figure><img src="image_3.png" /></figure>

B

<figure><img src="image_4.png" /></figure>

C

<figure><img src="image_5.png" /></figure>

FIGURE 1

Purified adult DETCs predominantly produce IL-13 during the early phase of activation. (A) Crude epidermal cells isolated from adult ear skin and DETCs purified from epidermal cells by positive magnetic selection using anti-integrin β7 mAb were stained with the indicated mAbs. Quadrant settings were determined by staining with isotype control mAbs. The numbers denote the percentages of cells in the respective quadrants. Representative profiles from two independent experiments are shown. In each experiment, cells pooled from 3-4 mice were analyzed. (B) Purified DETCs were stimulated with immobilized isotype control or anti-TCR mAb. Culture supernatants were harvested and replaced with fresh culture medium 24 hours after the start of stimulation, and DETCs were stimulated for an additional 24 hours. Cytokine levels in the supernatants were determined by ELISA. Representative data from two independent experiments are shown as the mean and SD of triplicate cultures. (C) mRNA expression of the indicated transcription factors in purified DETCs without stimulation (naive) and purified DETCs stimulated with immobilized anti-TCR mAb for 24 hours (activated) was analyzed in triplicate by real-time RT-PCR. Representative data from two independent experiments are shown as the mean and SD. Significant differences compared with naive DETCs are denoted with asterisks (*P < 0.05, **P < 0.01, ***P < 0.001).

These results confirmed that under steady-state conditions, DETCs
in the adult epidermis predominantly produce IL-13 during the early
phase of activation. However, DETCs can produce IFN-γ during the
later phase of activation. Therefore, unlike type 2 CD4⁺ helper T cells

(44), the IFN-γ-producing capacity of DETCs would not be repressed
by stable epigenetic modifications, as is the case for IL-17A-producing
CD27⁻ γδ T cells, which can produce IFN-γ in a certain inflammatory
microenvironment (45).
<figure><img src="image_4.png" /><figcaption>FIGURE 1</figcaption></figure>

Purified adult DETCs predominantly produce IL-13 during the early phase of activation. (A) Crude epidermal cells isolated from adult ear skin and DETCs purified from epidermal cells by positive magnetic selection using anti-integrin β7 mAb were stained with the indicated mAbs. Quadrant settings were determined by staining with isotype control mAbs. The numbers denote the percentages of cells in the respective quadrants. Representative profiles from two independent experiments are shown. In each experiment, cells pooled from 3-4 mice were analyzed. (B) Purified DETCs were stimulated with immobilized isotype control or anti-TCR mAb. Culture supernatants were harvested and replaced with fresh culture medium 24 hours after the start of stimulation, and DETCs were stimulated for an additional 24 hours. Cytokine levels in the supernatants were determined by ELISA. Representative data from two independent experiments are shown as the mean and SD of triplicate cultures. (C) mRNA expression of the indicated transcription factors in purified DETCs without stimulation (naive) and purified DETCs stimulated with immobilized anti-TCR mAb for 24 hours (activated) was analyzed in triplicate by real-time RT-PCR. Representative data from two independent experiments are shown as the mean and SD. Significant differences compared with naive DETCs are denoted with asterisks (*P < 0.05, **P < 0.01, ***P < 0.001).

These results confirmed that under steady-state conditions, DETCs
in the adult epidermis predominantly produce IL-13 during the early
phase of activation. However, DETCs can produce IFN-γ during the
later phase of activation. Therefore, unlike type 2 CD4⁺ helper T cells

(44), the IFN-γ-producing capacity of DETCs would not be repressed
by stable epigenetic modifications, as is the case for IL-17A-producing
CD27⁻ γδ T cells, which can produce IFN-γ in a certain inflammatory
microenvironment (45).
## Vγ3+ T cells lose CD27 expression immediately after migration to the dermis

Similar to embryonic day 17 (E17) mature Vγ3+ fetal thymocytes, circulating Vγ3+ T cells in the blood at E17 expressed CD27 (Figure 2A). As E17 fetal dermal Vγ3+ T cells did not express CD27 (Figure 2A), Vγ3+ T cells lost CD27 expression immediately after entering the dermis. A candidate that induces CD27 downregulation on Vγ3+ T cells is extracellular ATP, which would be abundant in the perinatal skin, because CD27 on T cells is rapidly shed and downregulated upon treatment with ATP (46). ATP treatment of E17 Vγ3+ fetal thymocytes resulted in rapid downregulation of CD27 (Figure 2B). As Vγ3+ T cells in day 1 (D1) neonatal epidermis that lacked CD27 expression (Figure 2A) could produce IFN-γ (Figure 3), CD27 expression and IFN-γ-producing capacity were not perfectly correlated in Vγ3+ T cells.

## Vγ3+ T cells differentiate from preprogrammed IFN-γ producers into rapid IL-13 producers in the perinatal epidermis

To clarify when and where Vγ3+ T cells functionally switch from preprogrammed IFN-γ producers to rapid IL-13 producers, we analyzed cytokines produced by Vγ3+ T cells upon short-term stimulation during ontogeny. Vγ3+ T cells in the E18 fetal epidermis still predominantly

produced IFN-γ when stimulated with PMA/ionomycin for 4 hours (Figure 3). While IFN-γ-producing cells gradually decreased after birth, IL-13-producing cells gradually increased (Figure 3). As cells producing both IFN-γ and IL-13 transiently appeared in the neonatal epidermis (Figure 3), Vγ3+ T cells differentiated from preprogrammed IFN-γ producers into rapid IL-13 producers via intermediate IFN-γ/IL-13 producers during this period in the epidermis.

## Epidermal T cells expressing TCRs that recognize the self-ligand on epidermal keratinocytes predominantly produce IL-13

Thymic maturation of Vγ3+ T cells to IFN-γ-producing cells requires TCR signaling. As the proliferation of Vγ3+ T cells in the perinatal epidermis also relies on TCR signaling (47–49), we hypothesized that TCR signaling also induces the IL-13-producing capacity of Vγ3+ T cells in the perinatal epidermis.

To determine the role of TCR signaling in the induction of the IL-13-producing capacity of epidermal T cells, we analyzed cytokines produced by resident epidermal T cells of adult TCR δ-chain-deficient *Tcrd*⁻/⁻ mice and TCR Vδ1-chain-deficient *Tcrd*-VI⁻/⁻ mice. In *Tcrd*⁻/⁻ mice lacking all γδ T cells, the epidermal niches of DETCs are replaced by αβ T cells, but the αβ TCRs expressed on these epidermal T cells cannot recognize the self-ligand on epidermal keratinocytes (27, 30). In contrast, epidermal T

<figure><img src="image_6.png" /><figcaption>FIGURE 2</figcaption></figure>

Vγ3+ T cells lose CD27 expression immediately after migration to the dermis. (A) Cells isolated from the indicated tissues at the indicated embryonic (E) or postnatal day (D) were stained with anti-Vγ3 mAb and anti-CD27 or isotype control mAb. Vγ3+ T cells were gated, and CD27 expression is shown as open histograms. Shaded histograms indicate cells stained with isotype control mAb. Each profile is representative of three independent experiments. In each experiment, cells pooled from 3-8 fetuses and 1-2 postnatal mice were analyzed. (B) E17 fetal thymocytes were treated with PBS (control) or ATP for 30 minutes and stained with anti-Vγ3 mAb and anti-CD27 or isotype control mAb. Vγ3+ T cells were gated, and CD27 expression is shown as open histograms. Shaded histograms indicate cells stained with isotype control mAb. Representative profiles from three independent experiments are shown. In each experiment, cells pooled from 9-10 fetuses were analyzed. Relative mean fluorescence intensity (MFI) was determined as (geometric MFI of CD27)/(geometric MFI of isotype control) and is shown in each panel as the mean and SEM (n = 3). Compared with the control, ATP treatment significantly diminished CD27 expression (P < 0.01).
## Vγ3+ T cells lose CD27 expression immediately after migration to the dermis

Similar to embryonic day 17 (E17) mature Vγ3+ fetal thymocytes, circulating Vγ3+ T cells in the blood at E17 expressed CD27 (Figure 2A). As E17 fetal dermal Vγ3+ T cells did not express CD27 (Figure 2A), Vγ3+ T cells lost CD27 expression immediately after entering the dermis. A candidate that induces CD27 downregulation on Vγ3+ T cells is extracellular ATP, which would be abundant in the perinatal skin, because CD27 on T cells is rapidly shed and downregulated upon treatment with ATP (46). ATP treatment of E17 Vγ3+ fetal thymocytes resulted in rapid downregulation of CD27 (Figure 2B). As Vγ3+ T cells in day 1 (D1) neonatal epidermis that lacked CD27 expression (Figure 2A) could produce IFN-γ (Figure 3), CD27 expression and IFN-γ-producing capacity were not perfectly correlated in Vγ3+ T cells.

## Vγ3+ T cells differentiate from preprogrammed IFN-γ producers into rapid IL-13 producers in the perinatal epidermis

To clarify when and where Vγ3+ T cells functionally switch from preprogrammed IFN-γ producers to rapid IL-13 producers, we analyzed cytokines produced by Vγ3+ T cells upon short-term stimulation during ontogeny. Vγ3+ T cells in the E18 fetal epidermis still predominantly

produced IFN-γ when stimulated with PMA/ionomycin for 4 hours (Figure 3). While IFN-γ-producing cells gradually decreased after birth, IL-13-producing cells gradually increased (Figure 3). As cells producing both IFN-γ and IL-13 transiently appeared in the neonatal epidermis (Figure 3), Vγ3+ T cells differentiated from preprogrammed IFN-γ producers into rapid IL-13 producers via intermediate IFN-γ/IL-13 producers during this period in the epidermis.

## Epidermal T cells expressing TCRs that recognize the self-ligand on epidermal keratinocytes predominantly produce IL-13

Thymic maturation of Vγ3+ T cells to IFN-γ-producing cells requires TCR signaling. As the proliferation of Vγ3+ T cells in the perinatal epidermis also relies on TCR signaling (47–49), we hypothesized that TCR signaling also induces the IL-13-producing capacity of Vγ3+ T cells in the perinatal epidermis.

To determine the role of TCR signaling in the induction of the IL-13-producing capacity of epidermal T cells, we analyzed cytokines produced by resident epidermal T cells of adult TCR δ-chain-deficient *Tcrd*⁻/⁻ mice and TCR Vδ1-chain-deficient *Tcrd*-VI⁻/⁻ mice. In *Tcrd*⁻/⁻ mice lacking all γδ T cells, the epidermal niches of DETCs are replaced by αβ T cells, but the αβ TCRs expressed on these epidermal T cells cannot recognize the self-ligand on epidermal keratinocytes (27, 30). In contrast, epidermal T

<figure><img src="image_5.png" /><figcaption>FIGURE 2
Vγ3+ T cells lose CD27 expression immediately after migration to the dermis. (A) Cells isolated from the indicated tissues at the indicated embryonic (E) or postnatal day (D) were stained with anti-Vγ3 mAb and anti-CD27 or isotype control mAb. Vγ3+ T cells were gated, and CD27 expression is shown as open histograms. Shaded histograms indicate cells stained with isotype control mAb. Each profile is representative of three independent experiments. In each experiment, cells pooled from 3-8 fetuses and 1-2 postnatal mice were analyzed. (B) E17 fetal thymocytes were treated with PBS (control) or ATP for 30 minutes and stained with anti-Vγ3 mAb and anti-CD27 or isotype control mAb. Vγ3+ T cells were gated, and CD27 expression is shown as open histograms. Shaded histograms indicate cells stained with isotype control mAb. Representative profiles from three independent experiments are shown. In each experiment, cells pooled from 9-10 fetuses were analyzed. Relative mean fluorescence intensity (MFI) was determined as (geometric MFI of CD27)/(geometric MFI of isotype control) and is shown in each panel as the mean and SEM (n = 3). Compared with the control, ATP treatment significantly diminished CD27 expression (P < 0.01).</figcaption></figure>
<figure><img src="image_7.png" /><figcaption>FIGURE 3</figcaption></figure>

Vγ3+ T cells differentiate from preprogrammed IFN-γ producers into rapid IL-13 producers in the perinatal epidermis. (A) Cells isolated from the thymus or epidermis at the indicated time points were stimulated with PMA/ionomycin for 4 hours. Vγ3+ T cells were gated, and intracellular IFN-γ and IL-13 production was analyzed by flow cytometry. Quadrant settings were determined by staining with isotype control mAbs. The numbers denote the percentages of cells in the respective quadrants. Each profile is representative of three independent experiments. In each experiment, cells pooled from 5-8 fetuses and 1-3 postnatal mice were analyzed. (B) Quantification of (A). Data are expressed as the mean and SEM (n = 3). Significant differences compared with the epidermis at 6 weeks are denoted with asterisks (*P* < 0.05, **P* < 0.01, ***P* < 0.001).

cells of *Tcrd-V1*⁻/⁻ mice express diverse γδ TCRs that can recognize the self-ligand on epidermal keratinocytes (50).

Epidermal αβ T cells of adult *Tcrd*⁻/⁻ mice produced IFN-γ but not IL-13 or IL-17A upon PMA/ionomycin stimulation for 4 hours (Figure 4). In contrast, both epidermal Vγ3⁺ and Vγ2⁺ T cells of adult *Tcrd-V1*⁻/⁻ mice, the latter of which are biased to produce IL-17A in the dermis of wild-type mice (38, 51, 52), predominantly produced IL-13 but not IFN-γ or IL-17A (Figure 4). These results suggest that ligand-dependent TCR signaling in the epidermis is needed for the induction and/or maintenance of the rapid IL-13-producing capacity of epidermal T cells.

TCR signaling through the p38 MAPK pathway promotes the differentiation of neonatal DETCs into IL-13-producing cells, whereas mammalian target of rapamycin complex 1 (mTORC1) signaling suppresses differentiated IL-13-producing cells

To determine the role of TCR signaling in the functional differentiation of DETCs more directly, we used an *in vitro* differentiation model of neonatal DETCs. Neonatal epidermal cells were cultured under TCR stimulation in the presence of
<figure><img src="image_6.png" /><figcaption>FIGURE 3</figcaption></figure>

Vγ3+ T cells differentiate from preprogrammed IFN-γ producers into rapid IL-13 producers in the perinatal epidermis. (A) Cells isolated from the thymus or epidermis at the indicated time points were stimulated with PMA/ionomycin for 4 hours. Vγ3+ T cells were gated, and intracellular IFN-γ and IL-13 production was analyzed by flow cytometry. Quadrant settings were determined by staining with isotype control mAbs. The numbers denote the percentages of cells in the respective quadrants. Each profile is representative of three independent experiments. In each experiment, cells pooled from 5-8 fetuses and 1-3 postnatal mice were analyzed. (B) Quantification of (A). Data are expressed as the mean and SEM (n = 3). Significant differences compared with the epidermis at 6 weeks are denoted with asterisks (*P* < 0.05, **P* < 0.01, ***P* < 0.001).

cells of *Tcrd-V1*⁻/⁻ mice express diverse γδ TCRs that can recognize the self-ligand on epidermal keratinocytes (50).

Epidermal αβ T cells of adult *Tcrd*⁻/⁻ mice produced IFN-γ but not IL-13 or IL-17A upon PMA/ionomycin stimulation for 4 hours (Figure 4). In contrast, both epidermal Vγ3⁺ and Vγ2⁺ T cells of adult *Tcrd-V1*⁻/⁻ mice, the latter of which are biased to produce IL-17A in the dermis of wild-type mice (38, 51, 52), predominantly produced IL-13 but not IFN-γ or IL-17A (Figure 4). These results suggest that ligand-dependent TCR signaling in the epidermis is needed for the induction and/or maintenance of the rapid IL-13-producing capacity of epidermal T cells.

TCR signaling through the p38 MAPK pathway promotes the differentiation of neonatal DETCs into IL-13-producing cells, whereas mammalian target of rapamycin complex 1 (mTORC1) signaling suppresses differentiated IL-13-producing cells

To determine the role of TCR signaling in the functional differentiation of DETCs more directly, we used an *in vitro* differentiation model of neonatal DETCs. Neonatal epidermal cells were cultured under TCR stimulation in the presence of
<figure><img src="image_8.png" /></figure>

FIGURE 4
Epidermal T cells expressing TCRs that recognize the self-ligand on epidermal keratinocytes predominantly produce IL-13. Epidermal cells from the indicated adult mice were stimulated with PMA/ionomycin for 4 hours. Vγ3+ T cells (wild-type mice), Cβ+ T cells (Tcrd-/- mice), and Vγ3+ or Vγ2+ T cells (Tcrd-V1-/- mice) were gated, and intracellular IFN-γ, IL-13, and IL-17A production was analyzed by flow cytometry. Quadrant settings were determined by staining with isotype control mAbs. The numbers denote the percentages of cells in the respective quadrants. Each profile is representative of two to four independent experiments with a single mouse per experiment.

various inhibitors of signaling pathways downstream of TCR, and cytokine production by Vγ3+ T cells was analyzed after restimulation with PMA/ionomycin for 4 hours. Among the various inhibitors, only the p38 MAPK inhibitor SB203580 blocked the differentiation of IL-13-producing cells from IFN-γ-producing cells (Figure 5A). Therefore, it was found that TCR signaling through the p38 MAPK pathway promotes the functional switch of DETCs.

Interestingly, the addition of MEK1/2-ERK1/2, PI3K, mTORC1, and mTORC1/2 inhibitors increased IL-13-producing cells with minimal impact on IFN-γ-producing cells (Figure 5A). As both ERK and PI3K activate mTORC1 (53), mTORC1 activation might suppress differentiated IL-13-producing cells.

## The p38 MAPK inhibitor SB203580 blocks the maintenance of the rapid IL-13-producing capacity of adult DETCs in vivo

In the adult epidermis, TCRs on DETCs are triggered at steady state (30). Therefore, TCR-p38 MAPK signaling may also play a role in the maintenance of the IL-13-producing capacity of DETCs in the adult epidermis. Intradermal administration of the p38 MAPK inhibitor SB203580 24 hours before analysis decreased DETCs with the IL-13-producing capacity in the adult epidermis (Figures 5B, C). IL-13 mRNA expression in epidermal cells upon short-term stimulation with PMA/ionomycin in vitro (Figure 5D) and in response to tape-stripping in vivo (Figure 5E) was suppressed

by SB203580 pretreatment. As DETCs are the only cells in the epidermis that produce IL-13 (22), these results suggest that continuous TCR signaling through the p38 MAPK pathway is also needed for maintaining the rapid IL-13-producing capacity of adult DETCs under steady-state conditions.

## The metabolic switch in DETCs from high glycolytic capacity to higher mitochondrial dependence occurs between 2 and 4 weeks after birth

A recent study showed that IFN-γ-producing γδ T cells use glycolysis for proliferation and to maintain effector functions, but IL-17A-producing γδ T cells are dependent on mitochondrial oxidative phosphorylation and fatty acid oxidation (54). As mTORC1, which regulates cellular metabolism, suppressed IL-13-producing cells in our *in vitro* differentiation model (Figure 5A), the functional switch of DETCs from IFN-γ-producing cells to IL-13-producing cells may be associated with the metabolic switch from mTORC1-dependent metabolic pathways to those less dependent on mTORC1.

To analyze the metabolic profiles of DETCs at different ages, we used the recently developed flow cytometry-based method SCENITH™ (55). D2 neonatal and D14 DETCs displayed higher glucose dependence and higher glycolytic capacity than D21 and D28 adult DETCs (Figure 6A). By D21, DETCs showed low glucose dependence and low glycolytic capacity, with a subsequent high dependence on mitochondrial oxidative phosphorylation and fatty
<figure><img src="image_7.png" /></figure>

FIGURE 4
Epidermal T cells expressing TCRs that recognize the self-ligand on epidermal keratinocytes predominantly produce IL-13. Epidermal cells from the indicated adult mice were stimulated with PMA/ionomycin for 4 hours. Vγ3+ T cells (wild-type mice), Cβ+ T cells (Tcrd-/- mice), and Vγ3+ or Vγ2+ T cells (Tcrd-V1-/- mice) were gated, and intracellular IFN-γ, IL-13, and IL-17A production was analyzed by flow cytometry. Quadrant settings were determined by staining with isotype control mAbs. The numbers denote the percentages of cells in the respective quadrants. Each profile is representative of two to four independent experiments with a single mouse per experiment.

various inhibitors of signaling pathways downstream of TCR, and cytokine production by Vγ3+ T cells was analyzed after restimulation with PMA/ionomycin for 4 hours. Among the various inhibitors, only the p38 MAPK inhibitor SB203580 blocked the differentiation of IL-13-producing cells from IFN-γ-producing cells (Figure 5A). Therefore, it was found that TCR signaling through the p38 MAPK pathway promotes the functional switch of DETCs.

Interestingly, the addition of MEK1/2-ERK1/2, PI3K, mTORC1, and mTORC1/2 inhibitors increased IL-13-producing cells with minimal impact on IFN-γ-producing cells (Figure 5A). As both ERK and PI3K activate mTORC1 (53), mTORC1 activation might suppress differentiated IL-13-producing cells.

## The p38 MAPK inhibitor SB203580 blocks the maintenance of the rapid IL-13-producing capacity of adult DETCs in vivo

In the adult epidermis, TCRs on DETCs are triggered at steady state (30). Therefore, TCR-p38 MAPK signaling may also play a role in the maintenance of the IL-13-producing capacity of DETCs in the adult epidermis. Intradermal administration of the p38 MAPK inhibitor SB203580 24 hours before analysis decreased DETCs with the IL-13-producing capacity in the adult epidermis (Figures 5B, C). IL-13 mRNA expression in epidermal cells upon short-term stimulation with PMA/ionomycin in vitro (Figure 5D) and in response to tape-stripping in vivo (Figure 5E) was suppressed

by SB203580 pretreatment. As DETCs are the only cells in the epidermis that produce IL-13 (22), these results suggest that continuous TCR signaling through the p38 MAPK pathway is also needed for maintaining the rapid IL-13-producing capacity of adult DETCs under steady-state conditions.

## The metabolic switch in DETCs from high glycolytic capacity to higher mitochondrial dependence occurs between 2 and 4 weeks after birth

A recent study showed that IFN-γ-producing γδ T cells use glycolysis for proliferation and to maintain effector functions, but IL-17A-producing γδ T cells are dependent on mitochondrial oxidative phosphorylation and fatty acid oxidation (54). As mTORC1, which regulates cellular metabolism, suppressed IL-13-producing cells in our *in vitro* differentiation model (Figure 5A), the functional switch of DETCs from IFN-γ-producing cells to IL-13-producing cells may be associated with the metabolic switch from mTORC1-dependent metabolic pathways to those less dependent on mTORC1.

To analyze the metabolic profiles of DETCs at different ages, we used the recently developed flow cytometry-based method SCENITH™ (55). D2 neonatal and D14 DETCs displayed higher glucose dependence and higher glycolytic capacity than D21 and D28 adult DETCs (Figure 6A). By D21, DETCs showed low glucose dependence and low glycolytic capacity, with a subsequent high dependence on mitochondrial oxidative phosphorylation and fatty
<figure><img src="image_9.png" /></figure>

<figure><img src="image_10.png" /></figure>

<figure><img src="image_11.png" /></figure>

<figure><img src="image_12.png" /></figure>

<figure><img src="image_13.png" /></figure>

FIGURE 5

TCR signaling through the p38 MAPK pathway promotes the differentiation of neonatal DETCs into IL-13-producing cells *in vitro* and the maintenance of the rapid IL-13-producing capacity of adult DETCs *in vivo*, whereas mTORC1 signaling suppresses differentiated IL-13-producing cells *in vitro*. (A) Day 1 neonatal epidermal cells were stimulated with immobilized anti-TCR mAb in the presence of DMSO (control) or inhibitors of the indicated TCR signaling pathways for 5 days and rested for 2 days. After PMA/ionomycin stimulation for 4 hours, cytokine production by gated Vγ3+ T cells was analyzed by flow cytometry. Representative data from three independent experiments are shown as the mean and SEM (n = 3). Significant differences compared with the control are denoted with asterisks (*P* < 0.05, **P* < 0.01, ***P* < 0.001). (B–E) PBS (control) and the p38 MAPK inhibitor SB203580 were injected intradermally into each ear of an adult mouse. (B–D) Epidermal cells were isolated from each ear 24 hours after the injection and stimulated with PMA/ionomycin for 4 hours. (B) Cytokine production by gated Vγ3+ T cells was analyzed by flow cytometry. Quadrant settings were determined by staining with isotype control mAbs. The numbers denote the percentages of cells in the respective quadrants. Representative profiles from three independent experiments are shown. (C) Quantification of (B). Data are expressed as the mean and SEM (n = 3). Significant difference compared with the control is denoted with asterisks (**P* < 0.01). (D) IL-13 mRNA expression in epidermal cells after PMA/ionomycin stimulation was analyzed in triplicate by real-time RT-PCR. Representative data from two independent experiments are shown as the mean and SD. Significant difference compared with the control is denoted with an asterisk (*P* < 0.05). (E) IL-13 mRNA expression in epidermal cells isolated 4 hours after tape-stripping *in vivo* was analyzed in triplicate by real-time RT-PCR. Representative data from two independent experiments are shown as the mean and SD. Significant difference compared with the control is denoted with asterisks (**P* < 0.01).
<figure><img src="image_8.png" /></figure>

FIGURE 5

TCR signaling through the p38 MAPK pathway promotes the differentiation of neonatal DETCs into IL-13-producing cells *in vitro* and the maintenance of the rapid IL-13-producing capacity of adult DETCs *in vivo*, whereas mTORC1 signaling suppresses differentiated IL-13-producing cells *in vitro*. (A) Day 1 neonatal epidermal cells were stimulated with immobilized anti-TCR mAb in the presence of DMSO (control) or inhibitors of the indicated TCR signaling pathways for 5 days and rested for 2 days. After PMA/ionomycin stimulation for 4 hours, cytokine production by gated Vγ3+ T cells was analyzed by flow cytometry. Representative data from three independent experiments are shown as the mean and SEM (n = 3). Significant differences compared with the control are denoted with asterisks (*P* < 0.05, **P* < 0.01, ***P* < 0.001). (B–E) PBS (control) and the p38 MAPK inhibitor SB203580 were injected intradermally into each ear of an adult mouse. (B–D) Epidermal cells were isolated from each ear 24 hours after the injection and stimulated with PMA/ionomycin for 4 hours. (B) Cytokine production by gated Vγ3+ T cells was analyzed by flow cytometry. Quadrant settings were determined by staining with isotype control mAbs. The numbers denote the percentages of cells in the respective quadrants. Representative profiles from three independent experiments are shown. (C) Quantification of (B). Data are expressed as the mean and SEM (n = 3). Significant difference compared with the control is denoted with asterisks (**P* < 0.01). (D) IL-13 mRNA expression in epidermal cells after PMA/ionomycin stimulation was analyzed in triplicate by real-time RT-PCR. Representative data from two independent experiments are shown as the mean and SD. Significant difference compared with the control is denoted with an asterisk (*P* < 0.05). (E) IL-13 mRNA expression in epidermal cells isolated 4 hours after tape-stripping *in vivo* was analyzed in triplicate by real-time RT-PCR. Representative data from two independent experiments are shown as the mean and SD. Significant difference compared with the control is denoted with asterisks (**P* < 0.01).
<figure><img src="image_14.png" /></figure>

<figure><img src="image_15.png" /></figure>

<figure><img src="image_16.png" /><figcaption>FIGURE 6</figcaption></figure>

The metabolic switch in DETCs from high glycolytic capacity to higher mitochondrial dependence occurs between 2 and 4 weeks after birth. (A) Epidermal cells isolated at the indicated time points were analyzed without stimulation for the metabolic dependence or capacity of gated Vγ3⁺ T cells by SCENITH™. Representative data from two independent experiments are shown. In each experiment, cells pooled from 3-7 mice were analyzed in triplicate. Data are expressed as the mean and SD. Significant differences compared with D2 are denoted with asterisks (**P < 0.01, ***P < 0.001). (B) Epidermal cells were isolated from D2 neonatal mice and 7-week-old adult mice and analyzed without stimulation for intracellular p4E-BP1 expression in gated Vγ3⁺ T cells. Representative profiles from two independent experiments are shown. In each experiment, cells pooled from 3-4 neonatal mice and a single adult mouse were analyzed in triplicate. (C) Quantification of (B). Relative MFI was determined as (geometric MFI of p4E-BP1)/(geometric MFI of isotype control). Data are expressed as the mean and SD. Significant difference compared with D2 is denoted with an asterisk (*P < 0.05).

acid/amino acid oxidation (Figure 6A). This metabolic reprogramming took place between 2 and 4 weeks after birth (Figure 6A) and thus occurred later than the functional switch observed during the perinatal period (Figure 3). As DETCs have

been shown to cease massive proliferation in the epidermis by 2 weeks after birth in C57BL/6 mice (37), mTORC1 activity may be attenuated at this time and maintained at low levels in adult DETCs under steady-state conditions.
<figure><img src="image_9.png" /></figure>

<figure><img src="image_10.png" /></figure>

<figure><img src="image_11.png" /><figcaption>FIGURE 6</figcaption></figure>

The metabolic switch in DETCs from high glycolytic capacity to higher mitochondrial dependence occurs between 2 and 4 weeks after birth. (A) Epidermal cells isolated at the indicated time points were analyzed without stimulation for the metabolic dependence or capacity of gated Vγ3⁺ T cells by SCENITH™. Representative data from two independent experiments are shown. In each experiment, cells pooled from 3-7 mice were analyzed in triplicate. Data are expressed as the mean and SD. Significant differences compared with D2 are denoted with asterisks (**P < 0.01, ***P < 0.001). (B) Epidermal cells were isolated from D2 neonatal mice and 7-week-old adult mice and analyzed without stimulation for intracellular p4E-BP1 expression in gated Vγ3⁺ T cells. Representative profiles from two independent experiments are shown. In each experiment, cells pooled from 3-4 neonatal mice and a single adult mouse were analyzed in triplicate. (C) Quantification of (B). Relative MFI was determined as (geometric MFI of p4E-BP1)/(geometric MFI of isotype control). Data are expressed as the mean and SD. Significant difference compared with D2 is denoted with an asterisk (*P < 0.05).

acid/amino acid oxidation (Figure 6A). This metabolic reprogramming took place between 2 and 4 weeks after birth (Figure 6A) and thus occurred later than the functional switch observed during the perinatal period (Figure 3). As DETCs have

been shown to cease massive proliferation in the epidermis by 2 weeks after birth in C57BL/6 mice (37), mTORC1 activity may be attenuated at this time and maintained at low levels in adult DETCs under steady-state conditions.
## Attenuated mTORC1 activity in adult DETCs under steady-state conditions

To determine whether mTORC1 activity is diminished in adult DETCs under steady-state conditions, we compared the levels of phosphorylated 4E-BP1 (p4E-BP1), an mTORC1 downstream target, between neonatal and adult DETCs. Compared with D2 neonatal DETCs, p4E-BP1 levels were lower in adult DETCs (Figures 6B, C). Therefore, mTORC1 activity was found to be attenuated in adult DETCs under steady-state conditions.

## Glycolysis inhibition decreases IFN-γ-producing cells but not IL-13-producing cells in adult DETCs during the late phase of activation

Finally, we determined whether cellular metabolism regulates cytokine production by adult DETCs. In contrast to adult DETCs under steady-state conditions (Figure 6A), adult DETCs stimulated with anti-TCR mAb for 3 days *in vitro* showed high glycolytic capacity (Figure 7A). Although adult DETCs predominantly produce IL-13 during the early phase of activation, adult DETCs stimulated for 3 days *in vitro* produced both IL-13 and IFN-γ (Figures 7B, C). Inhibition of glycolysis with 2-deoxy-D-glucose (2-DG) decreased IFN-γ-producing cells but not IL-13-producing cells in adult DETCs stimulated with anti-TCR mAb for 3 days *in vitro* (Figures 7B, C). As TCR-stimulated DETCs cultured in the presence or absence of 2-DG had equivalent levels of p4E-BP1 (Figures 7D, E), inhibition of glycolysis did not alter mTORC1 activity in adult DETCs. Therefore, it was found that glycolysis acts downstream of mTORC1 in the regulation of DETC cytokine production.

## Discussion

We showed that preprogrammed IFN-γ-producing $V\gamma3^+$ T cells differentiate into rapid IL-13 producers in the perinatal epidermis. This functional switch was promoted by TCR signaling through the p38 MAPK pathway. The downstream substrate of p38 MAPK in perinatal DETCs is currently unknown, but a likely candidate is GATA3 because phosphorylation of GATA3 by p38 MAPK is crucial for GATA3 nuclear translocation and IL-13 production in type 2 helper T cells (56) and group 2 innate lymphoid cells (57). As TCR signaling activates p38 MAPK not only through the canonical MAPK cascade but also through direct phosphorylation of p38 MAPK on a non-canonical activating residue by LCK-ZAP70 (58), it is also important to identify the upstream signaling pathway of p38 MAPK in perinatal DETCs in future studies.

Factors present in the perinatal epidermal microenvironment other than TCR signaling may also contribute to the induction of the IL-13-producing capacity of DETCs. Thus far, we have not yet identified such a factor. Treatment of E17 fetal thymocytes with IL-2, IL-4, IL-7, IL-15, TGF-β, or ATP did not induce the IL-13-producing capacity of $V\gamma3^+$ T cells (unpublished data). We also confirmed that adult DETCs of both TSLP receptor-deficient mice

and wild-type mice treated with anti-IL-25 and anti-IL-33 mAbs *in vivo* produced IL-13 but not IFN-γ upon short-term PMA/ionomycin stimulation (unpublished data). Nevertheless, the involvement of cognate signaling through interactions between DETCs and epidermal keratinocytes has not been addressed and warrants further investigation.

We demonstrated that signaling through the p38 MAPK pathway was also needed for the maintenance of the rapid IL-13-producing capacity of adult DETCs under steady-state conditions. Although p38 MAPK activation by a receptor other than TCR could be responsible for this finding, our data are consistent with those of a recent study showing that chronic intradermal administration of anti-Skint1 mAb resulted in the loss of IL-13 expression by adult DETCs (59) and together support the notion that constitutive TCR signaling at steady state through Skint1-dependent recognition of the self-ligand expressed on healthy keratinocytes ('normality sensing') maintains DETCs in a poised state to rapidly respond to epidermal stress (59). DETCs primarily produce IL-13 when activated *in vivo* after exposure to a variety of environmental stressors (22) and even after acute upregulation of transgenic NKG2D ligands on epidermal keratinocytes (24). Therefore, IL-13 production can be triggered in steady-state DETCs not only by TCR signaling via stress-induced upregulation of the TCR ligand but also by signaling through other stress-sensing receptors, including NKG2D, to maintain epidermal homeostasis (22).

We identified a role of cellular metabolism in the regulation of cytokine production by adult DETCs. Proliferating neonatal DETCs had high glycolytic capacity, whereas adult DETCs at steady state were more dependent on mitochondrial metabolism. This metabolic switch occurred between 2 and 4 weeks after birth. This is consistent with the fact that DETCs cease massive postnatal proliferation at this time in C57BL/6 mice (37). Accordingly, mTORC1 activity in adult DETCs under steady-state conditions was attenuated compared with that in proliferating neonatal DETCs. Inhibition of glycolysis decreased adult DETCs producing IFN-γ during the late phase of activation. As the inhibition of glycolysis resulted in a relative increase in the frequency of IL-13-producing DETCs, IL-13-producing DETCs redifferentiated into IFN-γ-producing cells during the late phase of activation. The (re)acquisition of the IFN-γ-producing capacity would require higher energy fueled by mTORC1-dependent glycolysis through sustained signaling than IL-13 production. Conversely, the low glycolytic capacity of adult DETCs at steady state contributes to the predominant production of IL-13 over IFN-γ early after activation as the former is less dependent on glycolysis than the latter.

mTORC1 is needed for the proliferation and survival of peripheral $\gamma\delta$ T cells and is essential for the differentiation of both IFN-γ-producing and IL-17A-producing $\gamma\delta$ T cells (60). *In vitro* treatment of adult DETCs with a high dose (20 ng/mL) of rapamycin inhibits their proliferation and induces autophagy (61). Therefore, a basal level of mTORC1 activity through low levels of TCR signaling and/or IL-15 receptor signaling is essential for the maintenance of DETCs at steady state. Full activation of DETCs and IFN-γ production would require enhanced mTORC1 activity and high glycolytic capacity to meet increased metabolic
## Attenuated mTORC1 activity in adult DETCs under steady-state conditions

To determine whether mTORC1 activity is diminished in adult DETCs under steady-state conditions, we compared the levels of phosphorylated 4E-BP1 (p4E-BP1), an mTORC1 downstream target, between neonatal and adult DETCs. Compared with D2 neonatal DETCs, p4E-BP1 levels were lower in adult DETCs (Figures 6B, C). Therefore, mTORC1 activity was found to be attenuated in adult DETCs under steady-state conditions.

## Glycolysis inhibition decreases IFN-γ-producing cells but not IL-13-producing cells in adult DETCs during the late phase of activation

Finally, we determined whether cellular metabolism regulates cytokine production by adult DETCs. In contrast to adult DETCs under steady-state conditions (Figure 6A), adult DETCs stimulated with anti-TCR mAb for 3 days *in vitro* showed high glycolytic capacity (Figure 7A). Although adult DETCs predominantly produce IL-13 during the early phase of activation, adult DETCs stimulated for 3 days *in vitro* produced both IL-13 and IFN-γ (Figures 7B, C). Inhibition of glycolysis with 2-deoxy-D-glucose (2-DG) decreased IFN-γ-producing cells but not IL-13-producing cells in adult DETCs stimulated with anti-TCR mAb for 3 days *in vitro* (Figures 7B, C). As TCR-stimulated DETCs cultured in the presence or absence of 2-DG had equivalent levels of p4E-BP1 (Figures 7D, E), inhibition of glycolysis did not alter mTORC1 activity in adult DETCs. Therefore, it was found that glycolysis acts downstream of mTORC1 in the regulation of DETC cytokine production.

## Discussion

We showed that preprogrammed IFN-γ-producing $V\gamma3^+$ T cells differentiate into rapid IL-13 producers in the perinatal epidermis. This functional switch was promoted by TCR signaling through the p38 MAPK pathway. The downstream substrate of p38 MAPK in perinatal DETCs is currently unknown, but a likely candidate is GATA3 because phosphorylation of GATA3 by p38 MAPK is crucial for GATA3 nuclear translocation and IL-13 production in type 2 helper T cells (56) and group 2 innate lymphoid cells (57). As TCR signaling activates p38 MAPK not only through the canonical MAPK cascade but also through direct phosphorylation of p38 MAPK on a non-canonical activating residue by LCK-ZAP70 (58), it is also important to identify the upstream signaling pathway of p38 MAPK in perinatal DETCs in future studies.

Factors present in the perinatal epidermal microenvironment other than TCR signaling may also contribute to the induction of the IL-13-producing capacity of DETCs. Thus far, we have not yet identified such a factor. Treatment of E17 fetal thymocytes with IL-2, IL-4, IL-7, IL-15, TGF-β, or ATP did not induce the IL-13-producing capacity of $V\gamma3^+$ T cells (unpublished data). We also confirmed that adult DETCs of both TSLP receptor-deficient mice

and wild-type mice treated with anti-IL-25 and anti-IL-33 mAbs *in vivo* produced IL-13 but not IFN-γ upon short-term PMA/ionomycin stimulation (unpublished data). Nevertheless, the involvement of cognate signaling through interactions between DETCs and epidermal keratinocytes has not been addressed and warrants further investigation.

We demonstrated that signaling through the p38 MAPK pathway was also needed for the maintenance of the rapid IL-13-producing capacity of adult DETCs under steady-state conditions. Although p38 MAPK activation by a receptor other than TCR could be responsible for this finding, our data are consistent with those of a recent study showing that chronic intradermal administration of anti-Skint1 mAb resulted in the loss of IL-13 expression by adult DETCs (59) and together support the notion that constitutive TCR signaling at steady state through Skint1-dependent recognition of the self-ligand expressed on healthy keratinocytes ('normality sensing') maintains DETCs in a poised state to rapidly respond to epidermal stress (59). DETCs primarily produce IL-13 when activated *in vivo* after exposure to a variety of environmental stressors (22) and even after acute upregulation of transgenic NKG2D ligands on epidermal keratinocytes (24). Therefore, IL-13 production can be triggered in steady-state DETCs not only by TCR signaling via stress-induced upregulation of the TCR ligand but also by signaling through other stress-sensing receptors, including NKG2D, to maintain epidermal homeostasis (22).

We identified a role of cellular metabolism in the regulation of cytokine production by adult DETCs. Proliferating neonatal DETCs had high glycolytic capacity, whereas adult DETCs at steady state were more dependent on mitochondrial metabolism. This metabolic switch occurred between 2 and 4 weeks after birth. This is consistent with the fact that DETCs cease massive postnatal proliferation at this time in C57BL/6 mice (37). Accordingly, mTORC1 activity in adult DETCs under steady-state conditions was attenuated compared with that in proliferating neonatal DETCs. Inhibition of glycolysis decreased adult DETCs producing IFN-γ during the late phase of activation. As the inhibition of glycolysis resulted in a relative increase in the frequency of IL-13-producing DETCs, IL-13-producing DETCs redifferentiated into IFN-γ-producing cells during the late phase of activation. The (re)acquisition of the IFN-γ-producing capacity would require higher energy fueled by mTORC1-dependent glycolysis through sustained signaling than IL-13 production. Conversely, the low glycolytic capacity of adult DETCs at steady state contributes to the predominant production of IL-13 over IFN-γ early after activation as the former is less dependent on glycolysis than the latter.

mTORC1 is needed for the proliferation and survival of peripheral $\gamma\delta$ T cells and is essential for the differentiation of both IFN-γ-producing and IL-17A-producing $\gamma\delta$ T cells (60). *In vitro* treatment of adult DETCs with a high dose (20 ng/mL) of rapamycin inhibits their proliferation and induces autophagy (61). Therefore, a basal level of mTORC1 activity through low levels of TCR signaling and/or IL-15 receptor signaling is essential for the maintenance of DETCs at steady state. Full activation of DETCs and IFN-γ production would require enhanced mTORC1 activity and high glycolytic capacity to meet increased metabolic
<figure><img src="image_17.png" /><figcaption>B</figcaption></figure>

<figure><img src="image_18.png" /><figcaption>D</figcaption></figure>

<figure><img src="image_19.png" /><figcaption>C</figcaption></figure>

<figure><img src="image_20.png" /><figcaption>E</figcaption></figure>

<figure><img src="image_21.png" /></figure>

FIGURE 7

Inhibition of glycolysis decreases IFN-γ-producing cells but not IL-13-producing cells in adult DETCs during the late phase of activation. (A) Adult epidermal cells stimulated with anti-TCR mAb for 3 days *in vitro* were analyzed for the metabolic dependence or capacity of gated Vγ3+ T cells by SCENITH™. Representative data from two independent experiments are shown. In each experiment, cells pooled from 2 mice were analyzed in triplicate. Data are expressed as the mean and SD. (B) Adult epidermal cells were stimulated with anti-TCR mAb in the presence of DMSO (control) or 2-DG for 3 days *in vitro*. Vγ3+ T cells were gated, and intracellular IFN-γ and IL-13 production was analyzed by flow cytometry. Quadrant settings were determined by staining with isotype control mAbs. The numbers denote the percentages of cells in the respective quadrants. Representative profiles from two independent experiments are shown. In each experiment, cells pooled from 2 mice were analyzed in triplicate. (C) Quantification of (B). Data are expressed as the mean and SD. Significant differences compared with the control are denoted with asterisks (\*\*P < 0.01, \*\*\*P < 0.001). (D) Adult epidermal cells stimulated with anti-TCR mAb in the presence of DMSO (control) or 2-DG for 3 days *in vitro* were analyzed for intracellular p4E-BP1 expression in gated Vγ3+ T cells. Representative profiles from two independent experiments are shown. In each experiment, cells pooled from 2 mice were analyzed in triplicate. (E) Quantification of (D). Relative MFI was determined as (geometric MFI of p4E-BP1)/ (geometric MFI of isotype control). Data are expressed as the mean and SD. Compared with the control, 2-DG treatment did not diminish p4E-BP1 expression in stimulated DETCs (NS, not significant).
<figure><img src="image_12.png" /></figure>

<figure><img src="image_13.png" /></figure>

<figure><img src="image_14.png" /></figure>

<figure><img src="image_15.png" /></figure>

<figure><img src="image_16.png" /></figure>

FIGURE 7

Inhibition of glycolysis decreases IFN-γ-producing cells but not IL-13-producing cells in adult DETCs during the late phase of activation. (A) Adult epidermal cells stimulated with anti-TCR mAb for 3 days *in vitro* were analyzed for the metabolic dependence or capacity of gated Vγ3+ T cells by SCENITH™. Representative data from two independent experiments are shown. In each experiment, cells pooled from 2 mice were analyzed in triplicate. Data are expressed as the mean and SD. (B) Adult epidermal cells were stimulated with anti-TCR mAb in the presence of DMSO (control) or 2-DG for 3 days *in vitro*. Vγ3+ T cells were gated, and intracellular IFN-γ and IL-13 production was analyzed by flow cytometry. Quadrant settings were determined by staining with isotype control mAbs. The numbers denote the percentages of cells in the respective quadrants. Representative profiles from two independent experiments are shown. In each experiment, cells pooled from 2 mice were analyzed in triplicate. (C) Quantification of (B). Data are expressed as the mean and SD. Significant differences compared with the control are denoted with asterisks (\*\*P < 0.01, \*\*\*P < 0.001). (D) Adult epidermal cells stimulated with anti-TCR mAb in the presence of DMSO (control) or 2-DG for 3 days *in vitro* were analyzed for intracellular p4E-BP1 expression in gated Vγ3+ T cells. Representative profiles from two independent experiments are shown. In each experiment, cells pooled from 2 mice were analyzed in triplicate. (E) Quantification of (D). Relative MFI was determined as (geometric MFI of p4E-BP1)/ (geometric MFI of isotype control). Data are expressed as the mean and SD. Compared with the control, 2-DG treatment did not diminish p4E-BP1 expression in stimulated DETCs (NS, not significant).
needs. To avoid complete blocking of mTORC1 activity, we used a low dose (5 ng/mL) of rapamycin for inhibiting mTORC1 activation in our *in vitro* neonatal DETC differentiation model. Although low-dose rapamycin treatment was reported to activate mTORC2 (62), the involvement of mTORC2 in the increase in IL-13-producing cells by rapamycin treatment was unlikely in our experiments because the addition of Torin 1, which inhibits both mTORC1 and mTORC2, also increased IL-13-producing cells. In our *in vitro* differentiation model, inhibition of mTORC1-dependent glycolysis might prevent differentiated IL-13-producing cells from redifferentiating into IFN-γ-producing cells and result in an increase in IL-13-producing cells.

In summary, we demonstrated that TCR signaling through the p38 MAPK pathway promotes the differentiation of IL-13-producing Vγ3+ T cells in the perinatal epidermis and that constitutive TCR signaling at steady state is also needed for maintaining the rapid IL-13-producing capacity of adult DETCs. In addition, the low mTORC1 activity and low glycolytic capacity of adult DETCs at steady state also regulate the rapid IL-13 response and delayed IFN-γ production after activation. A variety of sometimes conflicting effector functions of DETCs have been identified (7, 20–25). The effector functions of DETCs may be fine-tuned by their metabolic states depending on the epidermal microenvironment. Whether the functions of DETCs other than cytokine production (e.g., growth factor production, cytotoxicity) are regulated by cellular metabolism remains to be determined in future studies.

# Materials and methods

## Mice

C57BL/6J mice were purchased from Japan SLC (Hamamatsu, Japan). Tcrd⁻/⁻ mice (63) were purchased from the Jackson Laboratory (Bar Harbor, ME). Tcrd-VI⁻/⁻ mice (50) were a gift from Yasunobu Yoshikai (Division of Host Defense, Medical Institute of Bioregulation, Kyusyu University, Fukuoka, Japan). TSLP-R⁻/⁻ mice (64) were a gift from Steven F. Ziegler (Benaroya Research Institute, Seattle, WA). All mice were bred and maintained on a C57BL/6 background in the animal facility of Kagoshima University under specific pathogen-free conditions. Female mice at 4-12 weeks of age were used as adult mice. Fetuses and postnatal mice younger than 4 weeks were used irrespective of sex. Fetuses were obtained from timed pregnant mice. The plug date was defined as embryonic day 0 (E0).

## Cells

To isolate epidermal cells, the skin was floated dermal-side down on 1% trypsin (Gibco, Waltham, MA) in PBS for 30 minutes at 37°C. The epidermis was separated and collected in Iscove's modified Dulbecco's medium (IMDM, Gibco) supplemented with 10% FCS (Sigma-Aldrich, St. Louis, MO) and 0.025% DNase I (Sigma-Aldrich). Single-cell suspensions were obtained by

mechanical agitation and sequential filtration through 70- and 30-µm nylon meshes.

DETCs were purified from epidermal cells by positive magnetic selection using an anti-integrin β7 mAb because DETCs are the only cells in the normal epidermis that express the integrin β7 chain (65). After preincubation with anti-CD16/CD32 mAb (clone 2.4G2; BD Biosciences, Franklin Lakes, NJ), cells were stained with PE-conjugated anti-integrin β7 mAb (clone M293, BD Biosciences), followed by incubation with magnetic particles conjugated with anti-PE mAb (BD IMagTM Anti-R-PE Magnetic Particles-DM, BD Biosciences). The labeled cells were isolated using the BD IMagTM Cell Separation Magnet (BD Biosciences) according to the manufacturer's instructions.

To isolate dermal cells, the skin was floated dermal-side down on 1.2 U/mL dispase II (Roche Diagnostics, Basel, Switzerland) in IMDM for 30 minutes at 37°C. After removing the epidermis, small pieces of the dermis were digested in IMDM containing 0.01% DNase I and 250 U/mL collagenase IV (Sigma-Aldrich) for 30 minutes in a shaking water bath at 37°C. The digested dermis was filtered through 70- and 30-µm nylon meshes.

Fetal thymocytes were obtained by teasing the thymic lobes with fine forceps and filtering through a 70-µm nylon mesh.

Blood was collected in 20 mM EDTA in PBS. Lymphocytes were isolated by density gradient centrifugation on Lympholyte™-M Cell Separation Media (Cedarlane Laboratories, Burlington, Canada) for 15 minutes at 1000 × g.

## Flow cytometry

Cells were resuspended in PBS supplemented with 2% FCS and 0.1% NaN₃. After preincubation with anti-CD16/CD32 mAb, cells were stained with the following mAbs: FITC-, PE-, BD Horizon™ BB700-, or biotin-conjugated anti-TCR Vγ3 (clone 536, BD Biosciences or BioLegend, San Diego, CA), FITC-conjugated anti-CD3 (clone 145-2C11, eBioscience, Waltham, MA), FITC-conjugated anti-IAᵇ (clone AF6-120.1, BD Biosciences), biotin-conjugated anti-TCR Cβ (clone H57-597, BD Biosciences), biotin-conjugated anti-CD27 (clone LG.7F9, eBioscience), BB700-conjugated anti-TCR Vγ2 (clone UC3-10A6, BD Biosciences), and FITC-, PE-, BB700-, or biotin-conjugated isotype control mAbs (BD Biosciences or eBioscience). Biotin-conjugated mAbs were visualized with FITC- or PE-Cy5™-conjugated streptavidin (SouthernBiotech, Birmingham, AL or BD Biosciences).

For intracellular cytokine staining, cells were stimulated with 25 ng/mL PMA (Sigma-Aldrich) and 1 µg/mL ionomycin (Sigma-Aldrich) in the presence of brefeldin A (GolgiPlug™, BD Biosciences) for 4 hours at 37°C. TCR-stimulated cells were incubated with brefeldin A for the last 4 hours. After surface staining, the cells were fixed and permeabilized using Cytofix/Cytoperm™ (BD Biosciences) for 20 minutes at 4°C. Cells were washed and stained in Perm/Wash™ buffer (BD Biosciences) with Alexa Fluor™ 488-conjugated anti-IFN-γ (clone XMG1.2, BD Biosciences), PE-conjugated anti-IL-13 (clone eBio13A, eBioscience), PE-conjugated anti-IL-17A (clone TC11-18H10, BD Biosciences), and Alexa Fluor™ 488- or PE-conjugated isotype control mAbs (BD Biosciences or eBioscience).
needs. To avoid complete blocking of mTORC1 activity, we used a low dose (5 ng/mL) of rapamycin for inhibiting mTORC1 activation in our *in vitro* neonatal DETC differentiation model. Although low-dose rapamycin treatment was reported to activate mTORC2 (62), the involvement of mTORC2 in the increase in IL-13-producing cells by rapamycin treatment was unlikely in our experiments because the addition of Torin 1, which inhibits both mTORC1 and mTORC2, also increased IL-13-producing cells. In our *in vitro* differentiation model, inhibition of mTORC1-dependent glycolysis might prevent differentiated IL-13-producing cells from redifferentiating into IFN-γ-producing cells and result in an increase in IL-13-producing cells.

In summary, we demonstrated that TCR signaling through the p38 MAPK pathway promotes the differentiation of IL-13-producing Vγ3+ T cells in the perinatal epidermis and that constitutive TCR signaling at steady state is also needed for maintaining the rapid IL-13-producing capacity of adult DETCs. In addition, the low mTORC1 activity and low glycolytic capacity of adult DETCs at steady state also regulate the rapid IL-13 response and delayed IFN-γ production after activation. A variety of sometimes conflicting effector functions of DETCs have been identified (7, 20–25). The effector functions of DETCs may be fine-tuned by their metabolic states depending on the epidermal microenvironment. Whether the functions of DETCs other than cytokine production (e.g., growth factor production, cytotoxicity) are regulated by cellular metabolism remains to be determined in future studies.

# Materials and methods

## Mice

C57BL/6J mice were purchased from Japan SLC (Hamamatsu, Japan). Tcrd⁻/⁻ mice (63) were purchased from the Jackson Laboratory (Bar Harbor, ME). Tcrd-VI⁻/⁻ mice (50) were a gift from Yasunobu Yoshikai (Division of Host Defense, Medical Institute of Bioregulation, Kyusyu University, Fukuoka, Japan). TSLP-R⁻/⁻ mice (64) were a gift from Steven F. Ziegler (Benaroya Research Institute, Seattle, WA). All mice were bred and maintained on a C57BL/6 background in the animal facility of Kagoshima University under specific pathogen-free conditions. Female mice at 4-12 weeks of age were used as adult mice. Fetuses and postnatal mice younger than 4 weeks were used irrespective of sex. Fetuses were obtained from timed pregnant mice. The plug date was defined as embryonic day 0 (E0).

## Cells

To isolate epidermal cells, the skin was floated dermal-side down on 1% trypsin (Gibco, Waltham, MA) in PBS for 30 minutes at 37°C. The epidermis was separated and collected in Iscove's modified Dulbecco's medium (IMDM, Gibco) supplemented with 10% FCS (Sigma-Aldrich, St. Louis, MO) and 0.025% DNase I (Sigma-Aldrich). Single-cell suspensions were obtained by

mechanical agitation and sequential filtration through 70- and 30-µm nylon meshes.

DETCs were purified from epidermal cells by positive magnetic selection using an anti-integrin β7 mAb because DETCs are the only cells in the normal epidermis that express the integrin β7 chain (65). After preincubation with anti-CD16/CD32 mAb (clone 2.4G2; BD Biosciences, Franklin Lakes, NJ), cells were stained with PE-conjugated anti-integrin β7 mAb (clone M293, BD Biosciences), followed by incubation with magnetic particles conjugated with anti-PE mAb (BD IMag™ Anti-R-PE Magnetic Particles-DM, BD Biosciences). The labeled cells were isolated using the BD IMag™ Cell Separation Magnet (BD Biosciences) according to the manufacturer's instructions.

To isolate dermal cells, the skin was floated dermal-side down on 1.2 U/mL dispase II (Roche Diagnostics, Basel, Switzerland) in IMDM for 30 minutes at 37°C. After removing the epidermis, small pieces of the dermis were digested in IMDM containing 0.01% DNase I and 250 U/mL collagenase IV (Sigma-Aldrich) for 30 minutes in a shaking water bath at 37°C. The digested dermis was filtered through 70- and 30-µm nylon meshes.

Fetal thymocytes were obtained by teasing the thymic lobes with fine forceps and filtering through a 70-µm nylon mesh.

Blood was collected in 20 mM EDTA in PBS. Lymphocytes were isolated by density gradient centrifugation on Lympholyte™-M Cell Separation Media (Cedarlane Laboratories, Burlington, Canada) for 15 minutes at 1000 × g.

## Flow cytometry

Cells were resuspended in PBS supplemented with 2% FCS and 0.1% NaN₃. After preincubation with anti-CD16/CD32 mAb, cells were stained with the following mAbs: FITC-, PE-, BD Horizon™ BB700-, or biotin-conjugated anti-TCR Vγ3 (clone 536, BD Biosciences or BioLegend, San Diego, CA), FITC-conjugated anti-CD3 (clone 145-2C11, eBioscience, Waltham, MA), FITC-conjugated anti-IAᵇ (clone AF6-120.1, BD Biosciences), biotin-conjugated anti-TCR Cβ (clone H57-597, BD Biosciences), biotin-conjugated anti-CD27 (clone LG.7F9, eBioscience), BB700-conjugated anti-TCR Vγ2 (clone UC3-10A6, BD Biosciences), and FITC-, PE-, BB700-, or biotin-conjugated isotype control mAbs (BD Biosciences or eBioscience). Biotin-conjugated mAbs were visualized with FITC- or PE-Cy5™-conjugated streptavidin (SouthernBiotech, Birmingham, AL or BD Biosciences).

For intracellular cytokine staining, cells were stimulated with 25 ng/mL PMA (Sigma-Aldrich) and 1 µg/mL ionomycin (Sigma-Aldrich) in the presence of brefeldin A (GolgiPlug™, BD Biosciences) for 4 hours at 37°C. TCR-stimulated cells were incubated with brefeldin A for the last 4 hours. After surface staining, the cells were fixed and permeabilized using Cytofix/Cytoperm™ (BD Biosciences) for 20 minutes at 4°C. Cells were washed and stained in Perm/Wash™ buffer (BD Biosciences) with Alexa Fluor™ 488-conjugated anti-IFN-γ (clone XMG1.2, BD Biosciences), PE-conjugated anti-IL-13 (clone eBio13A, eBioscience), PE-conjugated anti-IL-17A (clone TC11-18H10, BD Biosciences), and Alexa Fluor™ 488- or PE-conjugated isotype control mAbs (BD Biosciences or eBioscience).
For intracellular p4E-BP1 staining, after surface staining, cells were fixed and permeabilized using Cytofix/Cytoperm™ and washed and stained in Perm/Wash™ buffer with Alexa Fluor™ 488-conjugated anti-p4E-BP1 (Thr37/46) mAb (clone 236B4, Cell Signaling Technology, Danvers, MA) or Alexa Fluor™ 488-conjugated isotype control mAb (Cell Signaling Technology).

After gating on forward and side scatters and viable cells as previously described (29, 65), cells were analyzed on a CytoFLEX flow cytometer with CytExpert software (Beckman Coulter, Brea, CA), and the data were analyzed using FlowJo™ software (Tree Star, Ashland, OR).

## TCR stimulation

Purified DETCs were stimulated on 96-well plates ($5 \times 10^4$ cells/well) coated with 10 µg/mL anti-TCR Cδ mAb (UC7-13D5, BD Biosciences) or isotype control mAb (BD Biosciences) in IMDM supplemented with 10% FCS and 50 µM 2-mercaptoethanol (Nacalai Tesque, Kyoto, Japan) for 24 hours at 37°C. Culture supernatants were harvested and replaced with fresh culture medium, and DETCs were stimulated for an additional 24 hours. Cytokine levels in the supernatants were determined using Quantikine™ ELISA kits (R&D Systems, Minneapolis, MN).

Epidermal cells were stimulated on 24-well plates ($1 \times 10^6$ cells/well) coated with 10 µg/mL anti-TCR Cδ mAb in IMDM supplemented with 10% FCS, 50 µM 2-mercaptoethanol, and 10 ng/mL recombinant mouse IL-2 (R&D Systems) in the presence of DMSO (Sigma-Aldrich) or 3 mM 2-DG (MedChemExpress, Monmouth Junction, NJ) for 3 days at 37°C. At the time of use, DETCs were harvested by incubation with 1 mM EDTA in PBS for 3 minutes.

## Real-time RT-PCR

Total RNA was extracted from the cells using the RNeasy™ Plus Mini kit (Qiagen, Venlo, Netherlands) and reverse transcribed using the SuperScript™ III First-Strand Synthesis System for RT-PCR (Invitrogen, Waltham, MA) with random hexamers. The cDNA was subjected to quantitative real-time PCR in triplicate using Thermal Cycler Dice™ Real Time System (Takara, Kusatsu, Japan) with FastStart Universal SYBR™ Green Master (Roche Diagnostics). All primers were purchased from Takara. Primer sequences are available upon request. The cycling conditions were 95°C for 10 minutes, followed by 40 cycles of 95°C for 15 seconds and 60°C for 1 minute. Threshold cycle (Ct) values were determined, and mRNA expression relative to that of the *Actβ* mRNA was calculated as $2^{-\Delta Ct}$.

## ATP treatment of fetal thymocytes

E17 fetal thymocytes were cultured on 24-well plates ($2 \times 10^6$ cells/well) in IMDM supplemented with 10% FCS and 50 µM 2-mercaptoethanol in the presence of PBS or 3 mM ATP (Sigma-Aldrich) for 30 minutes at 37°C.

## *In vitro* differentiation model of neonatal DETCs

Epidermal cells isolated from day 1 neonatal mice were stimulated on 24-well plates ($1 \times 10^6$ cells/well) coated with 10 µg/mL anti-TCR Cδ mAb in IMDM supplemented with 10% FCS, 50 µM 2-mercaptoethanol, and 10 ng/mL recombinant mouse IL-2 for 5 days at 37°C. Various inhibitors of signaling pathways downstream of TCR were added during this period. Cells were harvested and rested on uncoated plates in the same culture medium without inhibitors for 2 days at 37°C to allow the recovery of TCR expression before restimulation with PMA/ionomycin followed by intracellular cytokine staining.

Optimal concentrations of the inhibitors were predetermined as the maximum concentrations that did not affect viable $Vγ3^+$ T-cell yields after the cultures. The following inhibitors were used at the indicated concentrations: calcineurin inhibitor cyclosporin A (0.01 µM; Cell Signaling Technology), MEK1/2-ERK1/2 inhibitor U0126 (5 µM, Cell Signaling Technology), p38 MAPK inhibitor SB203580 (10 µM, Cell Signaling Technology), JNK inhibitor SP600125 (5 µM, Cell Signaling Technology), PKCθ inhibitor sotrostaurin (0.1 µM; Abcam, Cambridge, UK), PI3K inhibitor LY294002 (5 µM, Cell Signaling Technology), mTORC1 inhibitor rapamycin (5 ng/mL, Sigma-Aldrich), and mTORC1/2 inhibitor Torin 1 (0.05 µM, Cell Signaling Technology).

## *In vivo* treatment with the p38 MAPK inhibitor and tape-stripping

PBS or 10 µg SB203580 in 25 µL PBS was administered by intradermal injections into the dorsal and ventral sides of the ear pinna using a 29-gauge needle under inhalation anesthesia. Epidermal cells were isolated 24 hours after the injection and stimulated for 4 hours with PMA/ionomycin. To activate DETCs *in situ* by mild tissue abrasion induced by tape-stripping, the stratum corneum was removed from both sides of the earlobe by application and removal of cellophane tape (Scotch™, 3M, St. Paul, MN) seven times (22, 24) 24 hours after the injection. Epidermal cells were isolated 4 hours after tape stripping.

## Measurement of metabolic dependence and capacity

SCENITH™ was performed as previously described (55) using the SCENITH™ kit containing all reagents and protocols (obtained from www.scenith.com/try-it). Briefly, cells were treated on 24-well plates ($1 \times 10^6$ cells/well) with DMSO (control), 100 mM 2-DG, 1 µM oligomycin, or a combination of 2-DG and oligomycin for 40 minutes (for resting cells) or 30 minutes (for activated cells) at 37°C. Puromycin (10 µg/mL) was added for 40 minutes (for resting cells)
For intracellular p4E-BP1 staining, after surface staining, cells were fixed and permeabilized using Cytofix/Cytoperm™ and washed and stained in Perm/Wash™ buffer with Alexa Fluor™ 488-conjugated anti-p4E-BP1 (Thr37/46) mAb (clone 236B4, Cell Signaling Technology, Danvers, MA) or Alexa Fluor™ 488-conjugated isotype control mAb (Cell Signaling Technology).

After gating on forward and side scatters and viable cells as previously described (29, 65), cells were analyzed on a CytoFLEX flow cytometer with CytExpert software (Beckman Coulter, Brea, CA), and the data were analyzed using FlowJo™ software (Tree Star, Ashland, OR).

## TCR stimulation

Purified DETCs were stimulated on 96-well plates ($5 \times 10^4$ cells/well) coated with 10 µg/mL anti-TCR Cδ mAb (UC7-13D5, BD Biosciences) or isotype control mAb (BD Biosciences) in IMDM supplemented with 10% FCS and 50 µM 2-mercaptoethanol (Nacalai Tesque, Kyoto, Japan) for 24 hours at 37°C. Culture supernatants were harvested and replaced with fresh culture medium, and DETCs were stimulated for an additional 24 hours. Cytokine levels in the supernatants were determined using Quantikine™ ELISA kits (R&D Systems, Minneapolis, MN).

Epidermal cells were stimulated on 24-well plates ($1 \times 10^6$ cells/well) coated with 10 µg/mL anti-TCR Cδ mAb in IMDM supplemented with 10% FCS, 50 µM 2-mercaptoethanol, and 10 ng/mL recombinant mouse IL-2 (R&D Systems) in the presence of DMSO (Sigma-Aldrich) or 3 mM 2-DG (MedChemExpress, Monmouth Junction, NJ) for 3 days at 37°C. At the time of use, DETCs were harvested by incubation with 1 mM EDTA in PBS for 3 minutes.

## Real-time RT-PCR

Total RNA was extracted from the cells using the RNeasy™ Plus Mini kit (Qiagen, Venlo, Netherlands) and reverse transcribed using the SuperScript™ III First-Strand Synthesis System for RT-PCR (Invitrogen, Waltham, MA) with random hexamers. The cDNA was subjected to quantitative real-time PCR in triplicate using Thermal Cycler Dice™ Real Time System (Takara, Kusatsu, Japan) with FastStart Universal SYBR™ Green Master (Roche Diagnostics). All primers were purchased from Takara. Primer sequences are available upon request. The cycling conditions were 95°C for 10 minutes, followed by 40 cycles of 95°C for 15 seconds and 60°C for 1 minute. Threshold cycle (Ct) values were determined, and mRNA expression relative to that of the *Actβ* mRNA was calculated as $2^{-\Delta Ct}$.

## ATP treatment of fetal thymocytes

E17 fetal thymocytes were cultured on 24-well plates ($2 \times 10^6$ cells/well) in IMDM supplemented with 10% FCS and 50 µM 2-mercaptoethanol in the presence of PBS or 3 mM ATP (Sigma-Aldrich) for 30 minutes at 37°C.

## *In vitro* differentiation model of neonatal DETCs

Epidermal cells isolated from day 1 neonatal mice were stimulated on 24-well plates ($1 \times 10^6$ cells/well) coated with 10 µg/mL anti-TCR Cδ mAb in IMDM supplemented with 10% FCS, 50 µM 2-mercaptoethanol, and 10 ng/mL recombinant mouse IL-2 for 5 days at 37°C. Various inhibitors of signaling pathways downstream of TCR were added during this period. Cells were harvested and rested on uncoated plates in the same culture medium without inhibitors for 2 days at 37°C to allow the recovery of TCR expression before restimulation with PMA/ionomycin followed by intracellular cytokine staining.

Optimal concentrations of the inhibitors were predetermined as the maximum concentrations that did not affect viable $Vγ3^+$ T-cell yields after the cultures. The following inhibitors were used at the indicated concentrations: calcineurin inhibitor cyclosporin A (0.01 µM; Cell Signaling Technology), MEK1/2-ERK1/2 inhibitor U0126 (5 µM, Cell Signaling Technology), p38 MAPK inhibitor SB203580 (10 µM, Cell Signaling Technology), JNK inhibitor SP600125 (5 µM, Cell Signaling Technology), PKCθ inhibitor sotrostaurin (0.1 µM; Abcam, Cambridge, UK), PI3K inhibitor LY294002 (5 µM, Cell Signaling Technology), mTORC1 inhibitor rapamycin (5 ng/mL, Sigma-Aldrich), and mTORC1/2 inhibitor Torin 1 (0.05 µM, Cell Signaling Technology).

## *In vivo* treatment with the p38 MAPK inhibitor and tape-stripping

PBS or 10 µg SB203580 in 25 µL PBS was administered by intradermal injections into the dorsal and ventral sides of the ear pinna using a 29-gauge needle under inhalation anesthesia. Epidermal cells were isolated 24 hours after the injection and stimulated for 4 hours with PMA/ionomycin. To activate DETCs *in situ* by mild tissue abrasion induced by tape-stripping, the stratum corneum was removed from both sides of the earlobe by application and removal of cellophane tape (Scotch™, 3M, St. Paul, MN) seven times (22, 24) 24 hours after the injection. Epidermal cells were isolated 4 hours after tape stripping.

## Measurement of metabolic dependence and capacity

SCENITH™ was performed as previously described (55) using the SCENITH™ kit containing all reagents and protocols (obtained from www.scenith.com/try-it). Briefly, cells were treated on 24-well plates ($1 \times 10^6$ cells/well) with DMSO (control), 100 mM 2-DG, 1 µM oligomycin, or a combination of 2-DG and oligomycin for 40 minutes (for resting cells) or 30 minutes (for activated cells) at 37°C. Puromycin (10 µg/mL) was added for 40 minutes (for resting cells)
or for the last 15 minutes (for activated cells) at 37°C. After surface
staining, the cells were fixed and permeabilized using Cytofix/
Cytoperm™ and washed and stained in Perm/Wash™ buffer
with Alexa Fluor 488-conjugated anti-puromycin mAb (clone
R4743L-E8) for 30 minutes at 4°C. The impact of the various
metabolic inhibitors was quantified as previously described (55).

Statistical analysis

Differences between the two groups were evaluated by t-test.
Dunnett's test was used for multiple comparisons to a control. All
reported *P* values are two-tailed, with a *P* value < 0.05 considered
significant. Statistical calculations were performed using JMP™
software (SAS Institute, Cary, NC).

Data availability statement

The raw data supporting the conclusions of this article will be
made available by the authors without undue reservation.

Ethics statement

The animal study was approved by the Animal Care Committee
of Kagoshima University. The study was conducted in accordance
with the local legislation and institutional requirements.

Author contributions

AI: Conceptualization, Investigation, Validation, Writing –
original draft, Visualization. KK: Conceptualization, Funding
acquisition, Investigation, Validation, Writing – original draft,
Writing – review & editing, Formal analysis, Visualization. AN-T:
Investigation, Validation, Writing – review & editing. RA: Funding

acquisition, Writing – review & editing, Methodology, Resources.
TK: Supervision, Writing – review & editing.

Funding

The author(s) declare financial support was received for the
research, authorship, and/or publication of this article. This work
was supported in part by JSPS KAKENHI Grant Numbers
JP15K09773 and JP18K08302 to KK. We also acknowledge the
ANR for ANR-20-CE14-0028-01 and ANR-22-CE15-0015-02
grants to RA.

Acknowledgments

We thank Yasunobu Yoshikai for the *Tcrd-V1*⁻¹⁄₂⁻ mice and
Steven F. Ziegler for the *TSLP-R*⁻¹⁄₂⁻ mice.

Conflict of interest

There are restrictions to the commercial use of SCENITH™ due
to a pending patent application (PCT/EP2020/060486).

The authors declare that the research was conducted in the
absence of any commercial or financial relationships that could be
construed as a potential conflict of interest.

Publisher's note

All claims expressed in this article are solely those of the authors
and do not necessarily represent those of their affiliated
organizations, or those of the publisher, the editors and the
reviewers. Any product that may be evaluated in this article, or
claim that may be made by its manufacturer, is not guaranteed or
endorsed by the publisher.

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12. Parker ME, Ciofani M. Regulation of γδ T cell effector diversification in the thymus. *Front Immunol.* (2020) 11:42. doi: 10.3389/fimmu.2020.00042

13. Jensen KDC, Su X, Shin S, Li L, Youssef S, Yamasaki S, et al. Thymic selection determines γδ T cell effector fate: antigen-naive cells make interleukin-17 and antigen-experienced cells make interferon γ. *Immunity*. (2008) 29:90–100. doi: 10.1016/j.immuni.2008.04.022

14. Ribot JC, deBarros A, Pang DJ, Neves JF, Peperzak V, Roberts SJ, et al. CD27 is a thymic determinant of the balance between interferon-γ- and interleukin 17-producing γδ T cell subsets. *Nat Immunol.* (2009) 10:427–36. doi: 10.1038/ni.1717

15. Turchinovich G, Hayday AC. Skint-1 identifies a common molecular mechanism for the development of interferon-γ-secreting versus interleukin-17-secreting γδ T cells. *Immunity*. (2011) 35:59–68. doi: 10.1016/j.immuni.2011.04.018

16. Muñoz-Ruiz M, Ribot JC, Grosso AR, Goncalves-Sousa N, Pamplona A, Pennington DJ, et al. TCR signal strength controls thymic differentiation of discrete proinflammatory γδ T cell subsets. *Nat Immunol.* (2016) 17:721–7. doi: 10.1038/ni.3424
or for the last 15 minutes (for activated cells) at 37°C. After surface
staining, the cells were fixed and permeabilized using Cytofix/
Cytoperm™ and washed and stained in Perm/Wash™ buffer
with Alexa Fluor 488-conjugated anti-puromycin mAb (clone
R4743L-E8) for 30 minutes at 4°C. The impact of the various
metabolic inhibitors was quantified as previously described (55).

Statistical analysis

Differences between the two groups were evaluated by t-test.
Dunnett's test was used for multiple comparisons to a control. All
reported *P* values are two-tailed, with a *P* value < 0.05 considered
significant. Statistical calculations were performed using JMP™
software (SAS Institute, Cary, NC).

Data availability statement

The raw data supporting the conclusions of this article will be
made available by the authors without undue reservation.

Ethics statement

The animal study was approved by the Animal Care Committee
of Kagoshima University. The study was conducted in accordance
with the local legislation and institutional requirements.

Author contributions

AI: Conceptualization, Investigation, Validation, Writing –
original draft, Visualization. KK: Conceptualization, Funding
acquisition, Investigation, Validation, Writing – original draft,
Writing – review & editing, Formal analysis, Visualization. AN-T:
Investigation, Validation, Writing – review & editing. RA: Funding

acquisition, Writing – review & editing, Methodology, Resources.
TK: Supervision, Writing – review & editing.

Funding

The author(s) declare financial support was received for the
research, authorship, and/or publication of this article. This work
was supported in part by JSPS KAKENHI Grant Numbers
JP15K09773 and JP18K08302 to KK. We also acknowledge the
ANR for ANR-20-CE14-0028-01 and ANR-22-CE15-0015-02
grants to RA.

Acknowledgments

We thank Yasunobu Yoshikai for the *Tcrd-V1*⁻¹⁄₂⁻ mice and
Steven F. Ziegler for the *TSLP-R*⁻¹⁄₂⁻ mice.

Conflict of interest

There are restrictions to the commercial use of SCENITH™ due
to a pending patent application (PCT/EP2020/060486).

The authors declare that the research was conducted in the
absence of any commercial or financial relationships that could be
construed as a potential conflict of interest.

Publisher's note

All claims expressed in this article are solely those of the authors
and do not necessarily represent those of their affiliated
organizations, or those of the publisher, the editors and the
reviewers. Any product that may be evaluated in this article, or
claim that may be made by its manufacturer, is not guaranteed or
endorsed by the publisher.

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20. Thelen F, Witherden DA. Get in touch with dendritic epithelial T cells! *Front Immunol.* (2020) 11:1656. doi: 10.3389/fimmu.2020.01656

21. Sharp LL, Jameson JM, Cauvi G, Havran WL. Dendritic epidermal T cells regulate skin homeostasis through local production of insulin-like growth factor 1. *Nat Immunol.* (2005) 6:73–9. doi: 10.1038/ni1152

22. Dalessandri T, Crawford G, Hayes M, Castro Seoane R, Strid J. IL-13 from intraepithelial lymphocytes regulates tissue homeostasis and protects against carcinogenesis in the skin. *Nat Commun.* (2016) 7:12080. doi: 10.1038/ncomms12080

23. Jameson J, Ugarte K, Chen N, Yachi P, Fuchs E, Boismenu R, et al. A role for skin γδ T cells in wound repair. *Science.* (2002) 296:747–9. doi: 10.1126/science.1069639

24. Strid J, Sobolev O, Zafirova B, Polic B, Hayday A. The intraepithelial T cell response to NKG2D-ligands links lymphoid stress surveillance to atopy. *Science.* (2011) 334:1293–7. doi: 10.1126/science.1211250

25. Girardi M, Oppenheim DE, Steele CR, Lewis JM, Glusac E, Filler R, et al. Regulation of cutaneous malignancy by γδ T cells. *Science.* (2001) 294:605–9. doi: 10.1126/science.1063916

26. Havran WL, Chien YH, Allison JP. Recognition of self antigens by skin-derived T cells with invariant γδ antigen receptors. *Science.* (1991) 252:1430–2. doi: 10.1126/ science.1828619

27. Jameson JM, Cauvi G, Witherden DA, Havran WL. A keratinocyte-responsive γδ TCR is necessary for dendritic epidermal T cell activation by damaged keratinocytes and maintenance in the epidermis. *J Immunol.* (2004) 172:3573–9. doi: 10.4049/ jimmunol.172.6.3573

28. Komori HK, Witherden DA, Kelly R, Sendaydiego K, Jameson JM, Teyton L, et al. Cutting edge: dendritic epidermal γδ T cell ligands are rapidly and locally expressed by keratinocytes following cutaneous wounding. *J Immunol.* (2012) 188:2972–6. doi: 10.4049/jimmunol.1100887

29. Ibusuki A, Kawai K, Yoshida S, Uchida Y, Nitahara-Takeuchi A, Kuroki K, et al. NKG2D triggers cytotoxicity in murine epidermal γδ T cells via PI3K-dependent Syk/ZAP70-independent signaling pathway. *J Invest Dermatol.* (2014) 134:396–404. doi: 10.1038/jid.2013.353

30. Chodaczek G, Papanna V, Zal MA, Zal T. Body-barrier surveillance by epidermal γδ TCRs. *Nat Immunol.* (2012) 13:272–82. doi: 10.1038/ni.2240

31. Boyden LM, Lewis JM, Barbee SD, Bas A, Girardi M, Hayday AC, et al. *Skint1*, the prototype of a newly identified immunoglobulin superfamily gene cluster, positively selects epidermal γδ T cells. *Nat Genet.* (2008) 40:656–62. doi: 10.1038/ng.108

32. Jandke A, Melandri D, Monin L, Ushakov DS, Laing AG, Vandourout P, et al. Butyrophilin-like proteins display combinatorial diversity in selecting and maintaining signature intraepithelial γδ T cell compartments. *Nat Commun.* (2020) 11:3769. doi: 10.1038/s41467-020-17557-y

33. Xiong N, Kang C, Raulet DH. Positive selection of dendritic epidermal γδ T cell precursors in the fetal thymus determines expression of skin-homing receptors. *Immunity.* (2004) 21:121–31. doi: 10.1016/j.immuni.2004.06.008

34. Jin Y, Xia M, Sun A, Saylor CM, Xiong N. CCR10 is important for the development of skin-specific γδ T cells by regulating their migration and location. *J Immunol.* (2010) 185:5723–31. doi: 10.4049/jimmunol.1001612

35. Xia M, Qi Q, Jin Y, Wiest DL, August A, Xiong N. Differential roles of IL-2-inducible T cell kinase-mediated TCR signals in tissue-specific localization and maintenance of skin intraepithelial T cells. *J Immunol.* (2010) 184:6807–14. doi: 10.4049/jimmunol.1000453

36. Romani N, Schuler G, Fritsch P. Ontogeny of Ia-positive and Thy-1-positive leukocytes of murine epidermis. *J Invest Dermatol.* (1986) 86:129–33. doi: 10.1111/ 1523-1747.ep12284135

37. Elbe A, Tschachler E, Steiner G, Binder A, Wolff K, Stingl G. Maturational steps of bone marrow-derived dendritic murine epidermal cells: phenotypic and functional studies on Langerhans cells and Thy-1(+) dendritic epidermal cells in the perinatal period. *J Immunol.* (1989) 143:2431–8. doi: 10.4049/jimmunol.143.8.2431

38. Cai Y, Shen X, Ding C, Qi C, Li K, Li X, et al. Pivotal role of dermal IL-17-producing γδ T cells in skin inflammation. *Immunity.* (2011) 35:596–610. doi: 10.1016/ j.immuni.2011.08.001

39. Macleod AS, Hemmers S, Garijo O, Chabod M, Mowen K, Witherden DA, et al. Dendritic epidermal T cells regulate skin antimicrobial barrier function. *J Clin Invest.* (2013) 123:4364–74. doi: 10.1172/JCI70064

40. Shibata K, Yamada H, Nakamura R, Sun X, Itsumi M, Yoshikai Y. Identification of CD25(+) γδ T cells as fetal thymus-derived naturally occurring IL-17 producers. *J Immunol.* (2008) 181:5940–7. doi: 10.4049/jimmunol.181.9.5940

41. Sugaya M, Nakamura K, Tamaki K. Interleukins 18 and 12 synergistically upregulate interferon-γ production by murine dendritic epidermal T cells. *J Invest Dermatol.* (1999) 113:350–4. doi: 10.1046/j.1523-1747.1999.00697.x

42. Wencker M, Turchinovich G, Di Marco Barros R, Deban L, Jandke A, Cope A, et al. Innate-like T cells straddle innate and adaptive immunity by altering antigen-receptor responsiveness. *Nat Immunol.* (2014) 15:80–7. doi: 10.1038/ni.2773

43. Omori M, Yamashita M, Inami M, Ukai-Tadenuma M, Kimura M, Nigo Y, et al. CD8 T cell-specific downregulation of histone hyperacetylation and gene activation of the IL-4 gene locus by ROG, repressor of GATA. *Immunity.* (2003) 19:281–94. doi: 10.1016/s1074-7613(03)00210-3

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