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# Six years of tularemia surveillance in a zoo

Lorraine Michelet, Maellys Kevin, Moulay Ali Cherfa, Christiane Mendy, Baptiste Mulot, Nora Madani

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## ► To cite this version:

Lorraine Michelet, Maellys Kevin, Moulay Ali Cherfa, Christiane Mendy, Baptiste Mulot, et al.. Six years of tularemia surveillance in a zoo. 10th International Conference on Tularemia, Sep 2023, Grenoble, France. anses-04266192

HAL Id: anses-04266192

https://anses.hal.science/anses-04266192

Submitted on 31 Oct 2023

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# Six years of tularemia surveillance in a zoo

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Lorraine Michelet<fnref n="1" />, Maellys Kevin<fnref n="1" />,², Moulay Ali Cherfa<fnref n="1" />, Christiane Mendy<fnref n="1" />, Baptiste Mulot³, Nora Madani<fnref n="1" />

The first cases were detected in 2015 and 2016 in two non-human primates at ZooPark de Beauval, followed by two human cases in the veterinary team, probably contaminated through blood sampling or autopsy of the animals.

* Since 2017, tularemia surveillance of suspect animals (n=72) in the park and in the surrounding wildlife (n=23)

* Introduction of a tularemia protocol for autopsies (PPE, disinfection, etc).

* Samples (lung, spleen, kidney and liver) from suspect animals are sent to the French Tularemia NRL for bacteriology (BAC) and molecular biology (PCR).

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<figure><img src="image_7.png" /><figcaption>Figure 1 : Map of the zoo showing the localisation of cases</figcaption></figure>

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Two different strains were identified using the HRM typing method on three animals.

* From 2021, an external company is employed to remove rodents from the park every two weeks.

* No human or animal cases of tularemia detected since 2020.

* Further WGS analysis may help to better understand the link between cases.

* Surveillance for this disease in the zoo should be maintained. Environmental investigations (water, soil, food) could help to identify areas at risk in case of a re-emergence of the disease.

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<footnote n="1">¹ Université Paris-Est, Anses, Animal Health Laboratory, National reference laboratory for Tularemia, Maisons-Alfort, France, ² DNAscript, ³ ZooParc de Beauval & Beauval Nature, Saint-Aignan 41110, France</footnote>