# Integration of molecular tools into the maritime pine breeding program in France

Jérôme Bartholomé, Marjorie Vidal, Annie Raffin, Isabelle Lesur Kupin Lesur, Christophe C. Boury, Erwan Guichoux, Adline Delcamp, Joost van Heerwaarden, Christophe Plomion, Laurent Bouffier

► To cite this version:

Jérôme Bartholomé, Marjorie Vidal, Annie Raffin, Isabelle Lesur Kupin Lesur, Christophe C. Boury, et al.. Integration of molecular tools into the maritime pine breeding program in France. ProCoGen Dissemination Workshop "from our labs to yours forest", Dec 2015, Orléans, France. 75 p. hal-02738881

HAL Id: hal-02738881

https://hal.inrae.fr/hal-02738881

Submitted on 2 Jun 2020

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# ProCoGen final open conference
## Promoting Conifer Genomic Resources
### 30th November – 2nd December 2015
Orléans, France

Conifers are key ecological species dominating many terrestrial landscapes, and they are among the largest terrestrial carbon sinks. Of significant economic importance, conifers are key sources for timber, paper and bio-energy worldwide. At social and scientific levels, there is an increasing awareness of the global change challenges affecting conifers.

In parallel, technological and methodological improvements have been attained and have benefited the conifer taxa, notably on high throughput analytical tools able to describe the variability and plasticity at different levels of integration (from genes up to phenotypes). These new advances can be used not only to improve our understanding of fundamental conifer adaptive biology, but also to address practical problems for the forest industry as well as problems related to the management of conifer forests in the context of global change.

Several international research initiatives have crystalized around these new advances, like next-generation DNA sequencing technologies, with a focus on unraveling fundamental and practical problems of conifer adaptability and domestication. **ProCoGen** is a project funded by the EC 7FP that develops integrative and multidisciplinary genomic research in conifers, using high-throughput platforms for sequencing, genotyping and doing functional analysis. The objective of **ProCoGen** is to unravel genome organization and to identify genes and gene networks controlling important ecological and economic traits, such as those related to environmentally driven tree reaction for growth, drought and cold stress tolerance, and thus provide tree breeders with tools for precise selection. **ProCoGen** as well as other parallel initiatives worldwide have produced already substantial findings deserving broad dissemination among scientist for fostering awareness and further collaboration in conifer research.

With this goal in mind, a **ProCoGen** final open conference will be held in Orleans (France) from November 30th to December 2nd 2015. The aim of this international event will be to serve as a showcase of main results achieved in the project, along with other internationally relevant achievements brought in by key invited speakers and general attendees. External researchers from similar initiatives worldwide, from complementary disciplines ranging from genomics, to molecular and population genetics, tree physiology and developmental biology, biochemistry, molecular and cell biology, bioinformatics and conifer breeders, are invited to present and discuss recent and relevant results on structural, functional, comparative and translational genomics of conifer species. Emphasis will be given to broaden the coverage of key actors, from public research institutes and Universities to privately funded research organizations. External and **ProCoGen** keynote speakers, oral and poster presentations form external attendees and **ProCoGen** members will be included in

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ProCoGen

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the program. The number of participants will be limited to 100-120. No registration fees will be demanded. A conference website will be available for registration and abstract submission.

This open conference will be held along with a **ProCoGen** Training Workshop on *"Practicalities of marker and genome-assisted selection"* and with a **ProCoGen** Dissemination Workshop on *"Transfer of genome-related tools to breeding programs"*. The TWS and DSW will be held on December 3rd 2015 and December 4th 2015, respectively.

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ProCoGen is financially supported by the European Commission under the 7th Framework Programme
# Integration of molecular tools into the maritime pine breeding program in France

Jérôme Bartholomé <fnref n="1" />,<fnref n="2" />, Marjorie Vidal <fnref n="1" />,<fnref n="2" />,<fnref n="3" />, Annie Raffin <fnref n="1" />,<fnref n="2" />, Isabelle Lesur <fnref n="1" />,<fnref n="2" />, Christophe Boury <fnref n="1" />,<fnref n="2" />, Erwan Guichoux <fnref n="1" />,<fnref n="2" />, Adline Delcamp <fnref n="1" />,<fnref n="2" />, Joost van Heerwaarden <fnref n="4" />, Marco Bink <fnref n="4" />, Fikret Isik <fnref n="5" />, Christophe Plomion <fnref n="1" />,<fnref n="2" />, Laurent Bouffier <fnref n="1" />,<fnref n="2" />*

Maritime pine breeding in France started in the 1960ies with the selection of 635 founders (called G0 trees) in forest stands located in the Aquitaine region. A classical recurrent selection strategy to improve growth and wood quality is currently implemented using both progeny testing for genetic evaluation and bi-parental mating for selection. In this presentation we detail the progress of the integration of molecular tools into the maritime pine breeding program. From the verification of genetic identity to genomic selection approaches via polymix breeding strategy, we will present examples at different level of implementation. SNP arrays for DNA fingerprinting were developed for forest managers and breeders. These tools were successfully used in a study case of polymix breeding strategy where an increase of accuracy for genetic parameters and a better estimation of breeding values for the progeny were obtained. In addition, two proof-of-concept studies of genomic selection were established and have shown medium to high accuracy for the two main traits of the breeding program: growth and stem straightness.

<footnote n="1">¹ INRA, UMR1202 BIOGECO, F-33610 Cestas, France</footnote>

<footnote n="2">² Univ. Bordeaux, BIOGECO, UMR 1202, F-33600 Pessac, France</footnote>

<footnote n="3">³ FCBA, Biotechnology and Advanced Silviculture Department, Genetics & Biotechnology team, 33610 Cestas, France</footnote>

<footnote n="4">⁴ Biometris, Wageningen University and Research Centre, NL-6700 AC Wageningen, Netherlands</footnote>

<footnote n="5">⁵ North Carolina State University, Department of Forestry and Environmental Resources, Raleigh, NC, USA</footnote>