# Erratum to: Dynamics of gene silencing during X inactivation using allele-specific RNA-seq

Hendrik Marks, Hindrik H. D. Kerstens, Tahsin Stefan Barakat, Erik Splinter, René A. M. Dirks, Guido van Mierlo, Onkar Joshi, Shuang-Yin Wang, Tomas Babak, Cornelis A. Albers, et al.

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Hendrik Marks, Hindrik H. D. Kerstens, Tahsin Stefan Barakat, Erik Splinter, René A. M. Dirks, et al.. Erratum to: Dynamics of gene silencing during X inactivation using allele-specific RNA-seq. Genome Biology, 2016, 17, 10.1186/s13059-016-0885-4. hal-02630193

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# Erratum to: Dynamics of gene silencing during X inactivation using allele-specific RNA-seq

Hendrik Marks<fnref n="1" />*, Hindrik H. D. Kerstens<fnref n="1" />, Tahsin Stefan Barakat³, Erik Splinter⁴, René A. M. Dirks<fnref n="1" />, Guido van Mierlo<fnref n="1" />, Onkar Joshi<fnref n="1" />, Shuang-Yin Wang<fnref n="1" />, Tomas Babak⁵, Cornelis A. Albers², Tüzer Kalkan⁶, Austin Smith⁶, Alice Jouneau⁷, Wouter de Laat⁴, Joost Gribnau³ and Hendrik G. Stunnenberg<fnref n="1" />*

After the publication of this work [1], we noticed there was an error in Fig. 5 where –1,0 and 1 are incorrectly displayed in the y-axis in panel b. Please see the corrected Fig. 5 below. We apologize for this error.

**Author details**

¹Radboud University, Faculty of Science, Department of Molecular Biology, Radboud Institute for Molecular Life Sciences (RIMLS), Nijmegen 6500HB, The Netherlands. ²Radboud University, Faculty of Science, Department of Molecular Developmental Biology, Radboud Institute for Molecular Life Sciences (RIMLS), Nijmegen 6500HB, The Netherlands. ³Department of Reproduction and Development, Erasmus MC, University Medical Center, Rotterdam, The Netherlands. ⁴Hubrecht Institute, University Medical Center Utrecht, Uppsalaan 8, Utrecht 3584CT, The Netherlands. ⁵Biology Department, Queen's University, Kingston, ON, Canada. ⁶Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK. ⁷INRA, UMR1198 Biologie du Développement et Reproduction, Jouy-en-Josas F-78350, France.

Received: 25 January 2016 Accepted: 25 January 2016

**References**

1. Marks H, Kerstens HH, Barakat TS, Splinter E, Dirks RAM, van Mierlo G, et al. Dynamics of gene silencing during X inactivation using allele-specific RNA-seq. Genome Biol. 2015;16:149.

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<footnote>* Correspondence: H.Marks@ncmls.ru.nl; H.Stunnenberg@ncmls.ru.nl</footnote>

<footnote n="1">¹Radboud University, Faculty of Science, Department of Molecular Biology, Radboud Institute for Molecular Life Sciences (RIMLS), Nijmegen 6500HB, The Netherlands</footnote>
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<figure><img src="image_8.png" /><figcaption>**Fig. 5** llele-specific RNA-seq on three NPC lines identifies three distal regions of genes that escape XCI. **a** Ratio of Xi/Xa (y-axis; for each of the three NPC lines sorted from highest to lowest) for genes showing a log2 ratio of at least −5. We set the cutoff for escape on 10 % relative expression from the Xi versus the Xa (log 2 ratio of > −3.32; similar to Yang et al. [37]). **b** Xi/Xa ratio of genes that escape XCI in all three NPC lines. **c** Distribution of the escape genes identified in *NPC_129-Xi* over the four clusters as characterized in Fig. 4a. **d** Localization of the escape genes within each NPC line over the linear X chromosome (see also Table 1). The black dots on the fourth row represent all X-linked genes for which high-confidence allele-specific ratios were obtained in NPCs. **e** Validation of the escape genes within the three escape regions by Sanger sequencing of cDNA. See Additional file 1: Figure S13 for the full panel of 13 genes that we validated, and for further details</figcaption></figure>