# The PHYTOSTIM Project (2021-2025): Toward an integrative Analysis of Phytostimulant Mode of Action and Acceptance

Cecile Cabasson, Nicolas Guibert, Sabri Najaï, Joana Jorly, C. Eyssan, Pierre Pétriacq, Frédéric Delmas, Michel Hernould, J.M. M Deogratias, Maïder Saint Jean, et al.

► To cite this version:

Cecile Cabasson, Nicolas Guibert, Sabri Najaï, Joana Jorly, C. Eyssan, et al. The PHYTOSTIM Project (2021-2025): Toward an integrative Analysis of Phytostimulant Mode of Action and Acceptance. XII International Agriculture Symposium "AGROSYM 2021", Oct 2021, Sarajevo, Bosnia and Herzegovina. pp.P80, Agrosym 2021 - Book of proceedings. hal-03383359

HAL Id: hal-03383359

https://hal.inrae.fr/hal-03383359

Submitted on 18 Oct 2021

HAL is a multi-disciplinary open access
archive for the deposit and dissemination of sci-
entific research documents, whether they are pub-
lished or not. The documents may come from
teaching and research institutions in France or
abroad, or from public or private research centers.

L'archive ouverte pluridisciplinaire HAL, est
destinée au dépôt et à la diffusion de documents
scientifiques de niveau recherche, publiés ou non,
émanant des établissements d'enseignement et de
recherche français ou étrangers, des laboratoires
publics ou privés.

<figure><img src="image_1.png" /></figure>
<figure><img src="image_2.png" /></figure>

RÉPUBLIQUE
FRANÇAISE
Liberté
Égalité
Fraternité

INRAe

<figure><img src="image_3.png" /></figure>

Biologie du fruit et Pathologie

<figure><img src="image_4.png" /></figure>

GREThA

Groupe de Recherche en
Économie Théorique et Appliquée
UMR CNRS 5113

<figure><img src="image_5.png" /></figure>

UMR/1332 Biologie du Fruit et Pathologie

UMR/5113 GREThA

ASTREDHOR Sud-Ouest

C. Cabasson, N. Guibert, S. Najaï, J. Jorly, C. Eyssan, P. Pétriacq, S. Prigeant, F. Delmas, M. Hernould, J.M. Deogratias, M. Saint-Jean, Y. Gibon

## Introduction

The aim of this study was to characterize the effect of an algae-based biostimulant on tomato production. For this, we investigated the plant development (root system, vegetative and reproductive part)

<figure><img src="image_6.png" /><figcaption>TOTAL RANDOMIZED TRIAL - 10 plants per experimental unit</figcaption></figure>

<figure><img src="image_7.png" /></figure>

10 plants per experimental unit; 3 blocks per condition; 30 plants per condition. (sowing week 16, transplanting and first biostimulant treatment week 22)

Conditions M1 (without biostimulant), M2 (Pure Stim® Van Ipperen France - Spray 0.2% at repotting, watering 6 days later then every 10 days), M3 (Pure Stim® Van Ipperen France - Root ball soaking for 5 minutes at 0.2% before repotting, then foliar spraying during flowering ~23 mL/plant).

In addition for M1 and M2 : 60 plants (6 plants destroyed per week over 9 weeks of culture)

Black box : Other treatments in block (10 plants per experimental unit)

## Methodology

* ✓ Summer 2021, ASTREDHOR SO greenhouse

* ✓ 210 tomato plants (cultivar M82)

* ✓ Application of Stim Pure Liquid® (2 types, M2 & M3)

* ✓ Descriptors statement during 9 weeks: plant growth, rooting index, fruit yield...

* ✓ Leaf and fruit samples collection for biochemical phenotyping

<figure><img src="image_8.png" /><figcaption>ROOTING INDEX
Week 25, 19 DAT</figcaption></figure>

<figure><img src="image_9.png" /></figure>

DAT = day after transplanting

<figure><img src="image_10.png" /><figcaption>FLOWER STAGE - TRUSS 1
Week 28</figcaption></figure>

* P<0,05, ANOVA and Tukey test

## Conclusion & Perspective

The descriptors presented here do not allow to predict the effect of the biostimulant on tomato plant. The perspective is to study the biomass composition of the leaves and fruit via non-targeted metabolomics (Near-infra red spectroscopy analysis in progress).

<figure><img src="image_11.png" /></figure>

Centre
Bordeaux Nouvelle-Aquitaine

<figure><img src="image_12.png" /></figure>

Université
de BORDEAUX

<figure><img src="image_13.png" /></figure>

ASTREDHOR
SUD-OUEST
GIE Fleurs et Plantes

<figure><img src="image_14.png" /></figure>

RÉGION
Nouvelle-
Aquitaine

# The PHYTOSTIM Project (2021-2025): Toward an integrative Analysis of Phytostimulant Mode of Action and Acceptance

Sustainable Agriculture
European obligation set out in Directive 2009/128/EC

Bordeaux Plant Sciences (BPS)

Program of the University of Bordeaux

Phytostim project

TOMATO YIELD
Week 32, 69 DAT

<figure><img src="image_15.png" /></figure>

DAT = day after transplanting

* P<0,05, ANOVA and Tukey test

<figure><img src="image_16.png" /></figure>

Source : https://tea.solgenomics.net/anatomy/tomography

## Background
The Regulation (EU) 2019/1009 is a framework for Community action to achieve a sustainable use of pesticides. Biostimulant and biocontrol use is full of promises for a less impacting agriculture on human health and the environment. The PHYTOSTIM project is part of BPS program of the University of Bordeaux, whose ambition is to better understand and regulate the mechanisms involved in the trade-offs between yield and resistance to biotic and abiotic stress of crops.

## Results & Discussion
Here we present a part of the results available for this study of the effect of biostimulant application on tomato under conditions of production (without any stress in summer 2021). The application of biostimulant specified by the supplier (M2) did not confer any benefit in comparison with the control (M1) for the development of the root system and the vegetative part.
We noticed a slight drop in reproductive performance of the fruit production when the biostimulant was applied by root ball soaking at the time of transplantation and a slightly higher variability of the rooting index.

<figure><img src="image_17.png" /></figure>

<figure><img src="image_18.png" /></figure>

<figure><img src="image_19.png" /></figure>

<figure><img src="image_20.png" /></figure>

CONTACT US:
cecile.cabasson@u-bordeaux.fr
maider.saint-jean@u-bordeaux.fr
https://www.u-bordeaux.com/News/Seven-major-research-programs-at-the-University-of-Bordeaux