# A Comprehensive Review on Cucurbits Yellow Stunting Disorder Virus (CYSDV) and their Management

Badri Lal Nagar, Sakshi Pandey, Himendra Raj Raghuvanshi, M Muhammad Suhaib Ismayil, Shwetank Singh, Vivek Thapliyal, Dhananjay Kushwaha,

Arun Kumar

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Badri Lal Nagar, Sakshi Pandey, Himendra Raj Raghuvanshi, M Muhammad Suhaib Ismayil, Shwetank Singh, et al.. A Comprehensive Review on Cucurbits Yellow Stunting Disorder Virus (CYSDV) and their Management. International Journal of Environment and Climate Change, 2023, 13 (10), pp.3574 - 3586. hal-05192336

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# A Comprehensive Review on Cucurbits
## Yellow Stunting Disorder Virus
(CYSDV) and their Management

Badri Lal Nagar<sup>a++</sup>, Sakshi Pandey<sup>b++</sup>,
Himendra Raj Raghuvanshi<sup>c++</sup>,
Muhammad Suhaib Ismayil M.<sup>d++</sup>, Shwetank Singh<sup>e++</sup>,
Vivek Thapliyal<sup>f++</sup>, Dhananjay Kushwaha<sup>g++</sup>
and Arun Kumar<sup>g++*</sup>

## Authors' contributions

This work was carried out in collaboration among all authors. All authors read and approved the final manuscript.

## Article Information

DOI: 10.9734/IJECC/2023/v13i103028

**Open Peer Review History:**

This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/105907

Received: 05/07/2023

Accepted: 11/09/2023

Published: 19/09/2023

## Review Article

<footnote><sup>a</sup> Department of Vegetable Science, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, Madhya Pradesh (474002), India.</footnote>

<footnote><sup>b</sup> Department of Plant Pathology, Banda University of Agriculture & Technology, Banda, Uttar Pradesh (210001), India.</footnote>

<footnote><sup>c</sup> Department of Plant Pathology, Chandra Shekhar Azad University of Agriculture & Technology, Kanpur, Uttar Pradesh (208002), India.</footnote>

<footnote><sup>d</sup> Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bangalore, Karnataka (560065), India.</footnote>

<footnote><sup>e</sup> Department of Plant Pathology, Acharya Narendra Deva University of Agriculture & Technology, Kumarganj, Ayodhya, Uttar Pradesh (224229), India.</footnote>

<footnote><sup>f</sup> Department of Vegetable Science, Govind Ballabh Pant University of Agriculture and Technology, Pant Nagar, Uttarakhand (474002), India.</footnote>

<footnote><sup>g</sup> Department of Entomology, Chandra Shekhar Azad University of Agriculture & Technology, Kanpur, Uttar Pradesh (208002), India.</footnote>

<footnote>++ Ph.D. Scholar;</footnote>

<footnote>*Corresponding author: E-mail: arunkumarbujhansi@gmail.com;</footnote>
ABSTRACT

Cucurbit Yellow Stunting Disorder Virus (CYSDV) represents a significant threat to global agriculture, particularly impacting the cultivation of cucurbit crops such as melons, squashes, and cucumbers. This comprehensive review explores the various dimensions of CYSDV, including its taxonomy, epidemiology, pathogenesis, diagnostic methods, management, ongoing research, and the broader social and economic implications. Beginning with an examination of CYSDV's classification and morphology, the review delineates the geographical distribution of the virus, its host range, transmission vectors, and environmental factors influencing its spread. It also outlines the mechanisms of infection, stages of disease development, symptoms in various cucurbit species, and the economic impact of the disease. The discussion extends to both traditional and molecular diagnostic techniques and the associated challenges. Different strategies for managing and controlling CYSDV are highlighted, including cultural practices, chemical methods, biological control, and integrated pest management approaches. The review emphasizes ongoing research initiatives and future perspectives in CYSDV research, considering technological innovations and potential limitations. The final sections focus on the broader social and economic context, exploring the impact of CYSDV on small and large-scale farming, international trade considerations, community engagement, and government initiatives. Through an integrated analysis, this review provides valuable insights into the multifaceted nature of CYSDV, its influence on agriculture, and the wider societal dynamics. The conclusion underscores the necessity of a coordinated, comprehensive approach that leverages scientific research, international collaboration, community involvement, and governmental support to address the challenges posed by CYSDV. Understanding the complexities of this virus is essential for developing effective strategies to ensure food security and economic stability in regions affected by this detrimental plant disease.

Keywords: CYSDV; cucurbits; epidemiology; molecular diagnostic and management.

1. INTRODUCTION

Cucurbit Yellow Stunting Disorder Virus (CYSDV) is a viral disease that has captured the attention of agricultural scientists and farmers alike. Being a member of the genus *Crinivirus* within the family *Closteroviridae*, this virus presents a severe threat to the growth and sustainability of various cucurbit plants [1]. The whitefly-transmitted virus, which can cause a wide range of symptoms including yellowing and stunting in cucurbit plants, was first identified in the Middle East during the 1970s and has since spread to different parts of the world [2]. CYSDV was first observed in the southeastern coast of Spain, on melon and cucumber grown under plastic. The virus was also found in the Canary Islands, Egypt, France, Israel, Jordan, Lebanon, Mexico, Morocco, Portugal, Saudi Arabia, Syria, Texas, Turkey, and the United Arab Emirates. The family of plants affected by this virus is collectively known as cucurbits. They belong to the family *Cucurbitaceae* and include species like melon, cucumber, squash, pumpkin, and many others. These plants are widely cultivated not only for their fruits but also for seeds, and they possess ornamental value in various cultures. As staple crops in many parts of the world, cucurbits play a critical role in human nutrition and the

economic well-being of farming communities. Understanding the definition and characteristics of CYSDV is crucial for developing effective strategies to combat the virus. Structurally, CYSDV is a single-stranded RNA virus and can be identified by its unique morphology under the microscope. Recent studies have shown a close relationship between CYSDV and other viruses within the *Crinivirus* genus, contributing to the broader understanding of these plant pathogens [3]. Cucurbits are an important part of agricultural systems worldwide. Their significance lies not only in their nutritional value but also in their contribution to various economic and cultural practices. In many tropical and subtropical regions, cucurbits are a primary source of vitamins and minerals. They are used in traditional medicines and cultural rituals in several communities [4]. The importance of studying CYSDV cannot be overstated. First, the economic implications of the virus are profound. It has been reported that CYSDV can cause up to 50% yield loss in some cucurbit crops, leading to financial strain for farmers and affecting the supply chain in regions dependent on cucurbit production. Second, the virus poses a serious threat to food security. In some areas where cucurbits are a major dietary component, the spread of CYSDV can lead to malnutrition and
associated health problems. Third, the management of CYSDV often requires the use of chemical pesticides, potentially impacting the environment and leading to long-term ecological issues [5]. The increasing globalization of trade and transportation has facilitated the spread of CYSDV across different regions and continents. The virus's presence has been reported in North America, Europe, and Asia, underscoring the need for coordinated international efforts to understand and control the disease. Climate change and changing agricultural practices also contribute to the complexity of managing CYSDV, requiring ongoing research and innovation [6]. Given these factors, the objective of studying CYSDV extends beyond mere academic interest. The current review aims to synthesize the existing body of knowledge related to CYSDV's taxonomy, structure, and epidemiology. By doing so, the review seeks to provide practical insights that can guide the development of diagnostic methods, management strategies, and policy decisions related to CYSDV. The specific goals include understanding the virus's pathogenesis, evaluating its economic impact, exploring innovative control measures, and assessing the broader social and technological implications of CYSDV research [7].

## 2. LITERATURE REVIEW

Cucurbit Yellow Stunting Disorder Virus (CYSDV) has been a focal point of scientific investigation for several decades. First identified in the 1970s in the Middle East, the CYSDV has since spread to different continents and became a significant concern for cucurbit production worldwide [8]. Initially, researchers struggled to properly categorize the virus due to its resemblance to other plant diseases, but through meticulous observation and scientific inquiry, they were able to classify and understand CYSDV in more detail. The history of CYSDV research took a significant turn when the virus spread to North America and Europe, resulting in increased research efforts aimed at studying its biology, transmission, control, and economic impact. These efforts were supported by advancements in technology, leading to more sophisticated and precise methods of examination and analysis [9]. Over the years, numerous studies have been conducted to unravel various aspects of CYSDV. Early research focused on understanding its transmission, host range, and symptoms. Key discoveries included the identification of whitefly,

particularly *Bemisia tabaci*, as the primary vector transmitting CYSDV, and the recognition of cucurbits as the primary hosts. Comprehensive studies of symptomatology, including yellowing, stunting, and leaf discoloration, were carried out, revealing a devastating economic impact in different regions due to significant yield losses [10]. The exploration of the virus's genetic structure provided profound insights into its genomic makeup and function. Sequencing of its RNA genome enabled researchers to comprehend more about the behavior and spread of CYSDV. Studies investigated various management strategies, such as cultural practices, chemical control, and breeding resistant varieties, to mitigate the impact of CYSDV, paving the way for a new era of disease management [11]. Various methodologies have been employed in the research on CYSDV. Field studies played an essential role in observing the virus's behavior in its natural environment, leading to essential insights into the epidemic's development. Laboratory techniques such as PCR, RT-PCR, and sequencing were critical in characterizing the virus at the molecular level. Epidemiological analyses, through spatial and temporal studies, helped to unravel the virus's spread and impact across regions, while experimental infections were used to understand host range and transmission mechanisms [12]. Despite extensive research, several gaps in the literature still exist. The detailed molecular interaction between CYSDV and its host plants remains only partially understood, requiring study to unravel the complexity of this interaction. Long-term control strategies, taking into account sustainability and environmental implications, have not been thoroughly explored. Additionally, variations in virus behavior in different climatic and geographical conditions have not been comprehensively studied, and this could provide understanding of the virus's adaptability and potential to spread [13]. There is also a lack of research on the socio-economic impact of CYSDV, examining the broader effects on rural communities and local economies. Another notable gap is the absence of coordinated international efforts to tackle CYSDV as a global issue. Shared knowledge across regions and collaboration between different stakeholders could lead to a more robust response to the threats posed by CYSDV [14].

## 3. CYSDV TAXONOMY AND STRUCTURE

The Cucurbit Yellow Stunting Disorder Virus (CYSDV) is a member of the family
<figure><img src="image_3.png" /></figure>

Image 1. (a) Strong symptoms of PepMV on tomato; (b) symptom of CYSDV on melon; (c) severe vein yellowing on cucumber infected by CVYV; (d) swelling at the graft union and death of the scion of a vine grafted on Kober 5BB, infected by Grapevine leafroll-associated virus 2; (e) a citrus grove affected by sudden death disease; and (f) a citrus plant killed by sudden death disease. (a) Courtesy of Dr. E. Moriones. (b) Courtesy of Dr. E. Moriones. (c) Courtesy of Dr. I. M. Cuadrado. (e, f) Courtesy of: Dr. J.Bove

Closteroviridae, a group known for its complexity and distinctive features among plant viruses [15]. The CYSDV belongs to the genus Crinivirus, distinguished by their unique genetic structures and the mode of transmission, primarily by whiteflies. The Criniviruses are complex and multipartite RNA viruses, with CYSDV itself being bipartite, comprising two single-stranded, positive-sense RNA genomes [16]. The morphology of CYSDV has been studied using electron microscopy, revealing the typical filamentous form common to other members of the Closteroviridae family. The virions of CYSDV are reported to be 800-850 nm in length and around 12 nm in diameter, consisting of a helical nucleocapsid enveloped in a lipid bilayer [17]. The structural proteins form a coat around the RNA genomes, and there are also non-structural proteins, which play roles in replication and other virus functions. The genome of CYSDV is organized into two RNA components, RNA1 and RNA2. RNA1 is the larger segment and primarily encodes proteins involved in virus replication and some structural proteins. It consists of replicase genes, helicase, methyltransferase, and other non-structural proteins necessary for the virus's life cycle [18]. RNA2, the smaller segment,

encodes proteins that form the coat protein and play crucial roles in virus movement within the plant host and the transmission by vector insects. The bipartite nature of the genome necessitates a coordinated infection process where both RNA segments must be delivered to the host cells. Studies of CYSDV's genome organization have uncovered a multifaceted system of control and function. Its RNA nature means that the genome acts as both the template for replication and the mRNA for protein translation [19]. The regulation of these processes is not fully understood, but recent research has begun to uncover the specific roles of individual genes and proteins in the virus's life cycle. It is clear that the genome organization of CYSDV is complex and highly specialized, reflecting its adaptation to its specific hosts and vectors [20]. Additionally, comparative genomic analyses have shown similarities and differences between CYSDV and other members of the Closteroviridae family, providing insights into its evolution and classification. These studies have also facilitated the development of molecular tools for the detection and management of CYSDV, contributing to the ongoing efforts to control this agriculturally significant disease [21].
## 4. EPIDEMIOLOGY

The geographical distribution of CYSDV has expanded significantly since it was first identified in the Middle East during the 1970s. Initially confined to the Middle Eastern countries such as Jordan and Saudi Arabia [22], the virus has since spread to North Africa, Europe, Asia, and the Americas. In the U.S., it has been detected in states such as California, Arizona, and Florida (Wintermantel et al., 2009). Its rapid spread has raised concerns about the potential economic impact on cucurbit production globally [23]. CYSDV primarily affects members of the Cucurbitaceae family, including squash, melon, cucumber, and pumpkin. Some studies have suggested that CYSDV may infect other non-cucurbitaceous plants under experimental conditions, but these hosts are not typically associated with natural infections. The host range's specificity has shaped the virus's spread and determined the affected agricultural areas, mostly in regions where cucurbits are cultivated extensively [24]. The primary transmission vector for CYSDV is the whitefly *Bemisia tabaci*, a well-known vector for various plant viruses. The virus is transmitted in a persistent, semi-persistent manner, meaning that the vector remains viruliferous for a certain period after feeding on an infected plant. Other whitefly species, such as *Trialeurodes vaporariorum*, have been identified as potential vectors, but *B. tabaci* is by far the most significant. Understanding the biology and ecology of this vector has become central to the efforts to manage and control CYSDV [25]. The spread of CYSDV is significantly influenced by environmental factors, particularly those that affect the abundance and behavior of its vector, *B. tabaci*. Temperature, humidity, and wind play essential roles in the distribution and movement of the whiteflies, and therefore, the spread of the virus. Warmer temperatures and higher humidity levels favor the reproduction and survival of whiteflies, leading to a higher risk of CYSDV infection. Cultural practices, such as irrigation and planting density, also indirectly affect the virus's spread by influencing the vector's habitat and food availability [26]. Additionally, global trade and transportation of infected plant material have contributed to the virus's geographical expansion. The introduction of infected plants into new areas can quickly lead to local outbreaks, especially in regions with suitable environmental conditions and the presence of the vector [27].

## 5. PATHOGENESIS AND SYMPTOMS

The infection mechanism of CYSDV is a multifaceted process that commences with the virus's transmission by the whitefly *Bemisia tabaci*. Once transmitted, the virus targets phloem cells in the host plant [28]. The virus's replication occurs in the cytoplasm of these cells, utilizing host machinery to synthesize viral RNA and proteins. CYSDV then moves from cell to cell through plasmodesmata, specialized channels that link adjacent plant cells [29]. The coat protein of CYSDV plays a vital role in vector transmission and cell-to-cell movement. Host factors also likely contribute to the infection process, although detailed mechanisms remain partially understood [30]. Recent studies have begun to unravel the interactions between viral proteins and host components, providing insights into the molecular details of CYSDV infection.

## 6. STAGES OF DISEASE DEVELOPMENT

The development of CYSDV infection in a host plant can be divided into several stages, including:

1. **Inoculation:** Whiteflies transmit CYSDV to the host plant, specifically to phloem cells [31].

2. **Replication:** The virus replicates inside the host cells, synthesizing RNA and proteins necessary for its propagation [32].

3. **Spread:** The virus spreads locally from cell to cell and then systemically throughout the plant, eventually reaching all parts of the host [33].

4. **Symptom Expression:** Symptoms begin to appear, initially as mild chlorosis, later becoming more pronounced [34].

5. **Secondary Spread:** Infected plants serve as reservoirs for secondary infections, propagating the disease within a crop [35].

## 7. SYMPTOMS IN VARIOUS CUCURBIT SPECIES

The symptoms of CYSDV can vary among different cucurbit species but generally include leaf yellowing, stunting, and reduced fruit quality and quantity [36]. In cucumbers, an interveinal chlorosis may occur, while in melons, symptoms might include yellowing, reduced fruit size, and altered ripening patterns. Squash plants may exhibit severe yellowing with leaf curling. The virus has a latency period during which symptoms may not be apparent, leading to challenges in early detection and management [37].
## 8. ECONOMIC IMPACT

CYSDV's impact on cucurbit production is substantial. Yield losses can range from moderate to severe, depending on factors such as infection stage, host susceptibility, and environmental conditions [38]. In regions where CYSDV is endemic, such as parts of the Middle East and Mediterranean, the virus has significantly affected cucurbit production, leading to losses in both quality and quantity. The economic implications extend beyond direct yield losses. Efforts to manage CYSDV, including vector control, monitoring, and development of resistant varieties, also incur significant costs. Global trade restrictions and quarantine measures to prevent the virus's spread contribute to the economic burden of this disease [39].

## 9. DIAGNOSTIC METHODS

### 9.1 Traditional Diagnostic Techniques

#### 9.1.1 Visual inspection

Visual inspection is the primary traditional method of diagnosing CYSDV. The appearance of symptoms like yellowing and stunting in cucurbits [40] can be indicative of CYSDV infection. However, visual inspection alone is not definitive due to symptom similarity with other diseases.

#### 9.1.2 Serological tests

Enzyme-Linked Immunosorbent Assay (ELISA) is commonly used for detecting CYSDV. It employs antibodies specific to CYSDV and provides relatively rapid and sensitive detection. Other serological techniques like ImmunoStrip® have been used for field diagnosis [41].

### 9.2 Molecular Diagnostic Methods

#### 9.2.1 Polymerase chain reaction (PCR)

PCR has become an essential tool for CYSDV diagnosis. Specific primers designed to amplify segments of CYSDV genome offer sensitivity and specificity [42]. Real-Time PCR enhances detection accuracy and quantification.

#### 9.2.2 Reverse transcription pcr (RT-PCR)

RT-PCR, which involves converting viral RNA to DNA before amplification, has been employed

successfully to detect CYSDV in various hosts [43]. It is especially useful when studying the virus's genetic diversity and infection dynamics.

#### 9.2.3 Next-generation sequencing (NGS)

NGS offers a comprehensive approach to detect and characterize CYSDV and other co-infecting pathogens [44]. It enables a broader understanding of virus evolution and epidemiology.

### 9.3 Limitations and Challenges in Diagnosis

#### 9.3.1 Overlap with other diseases

The symptoms of CYSDV can resemble other cucurbit diseases, leading to potential misdiagnosis [45]. Traditional methods may struggle to distinguish between CYSDV and other closely related viruses.

#### 9.3.2 Sensitivity and specificity issues

Traditional serological tests may sometimes lack sensitivity, especially in early-stage infections or low viral load conditions. Cross-reactivity with related viruses can lead to false positives [46].

#### 9.3.3 Resource-intensive molecular methods

While molecular techniques like PCR and NGS offer high accuracy, they require specialized equipment and trained personnel. This may limit their applicability in resource-poor settings or remote field locations [47].

#### 9.3.4 Need for continuous development

New CYSDV strains and emerging related viruses necessitate continuous updating of diagnostic methods and tools. Keeping up with the evolving landscape of CYSDV and other viruses poses an ongoing challenge [48].

## 10. MANAGEMENT AND CONTROL

### 10.1 Cultural Practices

The adoption of sound cultural practices has been instrumental in mitigating the impact of CYSDV in various cucurbit-growing regions. Crop rotation is considered one of the most effective measures for breaking the life cycle of the virus's vector, whiteflies. By planting non-host crops in rotation, the continuity of host plants is
disrupted, thus reducing whitefly populations and CYSDV spread [49]. The introduction of CYSDV-resistant cucurbit varieties has also proven effective in reducing disease incidence [50]. Strict sanitation measures, including the regular removal of infected plants and weeds that may host the virus, minimize the potential sources of CYSDV in the field [51]. Adjusting planting dates to coincide with lower whitefly populations has also been shown to reduce the risk of early-season infection.

## 10.2 Chemical Control Methods

Chemical control remains an essential strategy in managing CYSDV, particularly in regions where the virus is prevalent. The application of insecticides targeting the whitefly vector has proven effective in lowering the risk of virus transmission [52]. However, this approach requires careful selection and timing of insecticides to minimize negative impacts on beneficial insects. Soil fumigation with chemicals like metam sodium has also been used to suppress soil-borne stages of the whitefly, aiding in CYSDV control [53]. The use of reflective mulches, which deter whiteflies from landing on plants, has been found to reduce transmission effectively [54].

## 10.3 Biological Control Strategies

Biological control has emerged as a promising alternative to conventional chemical methods in the management of CYSDV. The introduction of natural enemies of whiteflies, such as predatory beetles or parasitoid wasps, has helped in controlling whitefly populations [55]. Research into biopesticides, utilizing microbial agents like Beauveria bassiana that infect and kill whiteflies, has offered an environmentally friendly control option [56]. Studies have explored antiviral compounds in plants that can be extracted and used to induce resistance in cucurbits against CYSDV.

## 10.4 Integrated Pest Management Approaches

Integrated Pest Management (IPM) approaches combine the strengths of cultural, chemical, and biological strategies, providing a comprehensive and sustainable solution to CYSDV control. Regular monitoring of whitefly populations and infection levels, along with defined intervention thresholds, enables timely and effective management [57]. The multitactic approach of

IPM, integrating cultural practices, insecticides, and biological controls, ensures more effective and sustainable CYSDV management, tailored to specific regional conditions [58]. Education and extension support to farmers on the latest techniques and practices for CYSDV management enhance the overall effectiveness of control strategies [59].

# 11. RESEARCH AND DEVELOPMENT

## 11.1 Ongoing Research Initiatives

The current research on CYSDV is extensive and multifaceted, focusing on different aspects of understanding and controlling the virus:

1. **Molecular Studies:** Researchers are continuously examining the molecular structure of CYSDV to understand its interaction with host plants [60].

2. **Vector Control:** Extensive research is ongoing to control the whitefly vector, including genetic studies to understand its biology and behavior [61].

3. **Resistant Crop Varieties:** Plant breeding techniques are being used to develop cucurbit varieties resistant to CYSDV [62].

4. **Integrated Management Strategies:** Research includes combining cultural, chemical, biological, and physical methods to develop sustainable disease management strategies [63].

## 11.2 Future Perspectives in CYSDV Research

Future research avenues show promising directions:

1. **Genomic Approaches:** Utilizing genomic tools to explore complex interactions between CYSDV, host plants, and vectors [64].

2. **Innovative Control Measures:** Including environmentally sustainable methods such as RNA interference technology and biological control agents [65].

3. **Climate Change Impact:** Investigating how global climate changes will affect the epidemiology of CYSDV and creating adaptive strategies [66].

## 11.3 Challenges and Limitations

Several challenges hinder the fight against CYSDV:
1. **Limited Genetic Resources:** Scarcity of naturally resistant sources is a major constraint in breeding CYSDV-resistant varieties [67].

2. **Complex Interactions:** The intricate relationships between the virus, host plants, and whitefly vector increase the complexity of disease management [68].

3. **Economic Factors:** Lack of funding and resources in areas where CYSDV is prevalent can impede research and control measures [69].

4. **Environmental Sustainability:** Striking a balance between effective control measures and environmental considerations is a continuous challenge [70].

## 11.4 Potential for Technological Innovations

Emerging technologies are providing new opportunities:

1. **Precision Agriculture Technologies:** Implementing remote sensing and GIS can enhance early detection and targeted control of CYSDV [71].

2. **Genetic Engineering and CRISPR/Cas Systems:** Developing CYSDV-resistant cucurbit varieties using genetic engineering techniques is promising [72].

3. **Big Data and AI:** Leveraging data analytics and artificial intelligence can provide insights into CYSDV epidemiology and predictive modeling [73].

## 12. SOCIAL AND ECONOMIC IMPLICATIONS

### 12.1 Impact on Small-scale and Large-scale Farming

Cucurbit Yellow Stunting Disorder Virus (CYSDV) poses significant threats to both small-scale and large-scale farming operations. For small-scale farmers, the emergence of CYSDV can be devastating.

1. **Small-scale Farming:** Many small-scale farmers depend on cucurbit crops such as melons, squashes, and cucumbers for their livelihoods. The loss of yield due to CYSDV infection can lead to economic hardship. Small farmers often lack access to advanced diagnostic tools and effective control methods, making them particularly vulnerable [74].

2. **Large-scale Farming:** Even in large-scale farming operations, CYSDV can cause substantial losses. The virus can spread rapidly across large areas, and controlling it requires significant investments in pesticides, resistant varieties, and other management practices [75]. The economic costs of managing CYSDV in large-scale farming can be substantial, affecting overall profitability [76].

### 12.2 International Trade Considerations

CYSDV has considerable implications for international trade, impacting both export and import markets:

1. **Export Markets:** Countries that export cucurbit products to international markets must adhere to stringent phytosanitary regulations. The presence of CYSDV can lead to trade barriers and loss of access to valuable markets [77]. Managing CYSDV to meet international standards can be costly, impacting the competitiveness of exports [78].

2. **Import Controls:** Countries that import cucurbit products must implement measures to prevent the introduction and spread of CYSDV. These measures can include inspections, quarantines, and restrictions, which add to the costs and complexity of international trade [79].

### 12.3 Community and Government Initiatives

Local community engagement can play a vital role in CYSDV management. Farmer education and community-led interventions can help in early detection and implementation of control measures. Collaboration and knowledge-sharing among farmers can lead to more effective and sustainable solutions [80]. Governments play a crucial role in managing CYSDV. Initiatives may include funding research, providing subsidies for control measures, enforcing quarantine regulations, and facilitating international cooperation [81]. Government policies can help support both small-scale and large-scale farmers, ensuring food security and economic stability [82].
## 13. CONCLUSION

The social and economic implications of Cucurbit Yellow Stunting Disorder Virus (CYSDV) are profound, affecting both small-scale and large-scale farming operations. The virus can cause significant economic hardships for farmers, disrupt international trade through stringent regulations, and necessitate community and government engagement in managing its spread and impact. Efforts to combat CYSDV must be multifaceted, encompassing scientific research, international collaboration, community involvement, and governmental support. The interconnectedness of CYSDV with broader societal dynamics emphasizes the critical need for a coordinated and comprehensive approach to ensure food security and economic stability.

## COMPETING INTERESTS

Authors have declared that no competing interests exist.

## REFERENCES

1. Tzanetakis IE, Martin RR, Wintermantel WM. Epidemiology of criniviruses: An emerging problem in world agriculture. Frontiers in Microbiology. 2013;4:119.

2. Navas-Castillo J, López-Moya JJ, Aranda MA. Whitefly-transmitted rna viruses that affect intensive vegetable production. Annals of Applied Biology. 2014;165(2):155-171.

3. Wintermantel WM, Gilbertson RL, McCreight JD, Natwick ET. Host-specific relationship between virus titer and whitefly transmission of Cucurbit yellow stunting disorder virus. Plant Disease. 2016;100(1):92-98.

4. Ozioma EOJ, Chinwe OAN. Herbal medicines in African traditional medicine. Herbal medicine. 2019;10:191-214.

5. Stansly PA, Natwick ET. Integrated systems for managing *Bemisia tabaci* in protected and open field agriculture. *Bemisia: Bionomics and management of a global pest*. 2010;467-497.

6. Krishnareddy M. Impact of climate change on insect vectors and vector-borne plant viruses and phytoplasma. In *Climate-resilient horticulture: adaptation and mitigation strategies*. 2013;255-277. India: Springer India.

7. Defendi HGT, da Silva Madeira L, Borschiver S. Analysis of the COVID-19 vaccine development process: An exploratory study of accelerating factors and innovative environments. Journal of Pharmaceutical Innovation. 2021;1-17.

8. Castle S, Palumbo J, Merten P, Cowden C, Prabhaker N. Effects of foliar and systemic insecticides on whitefly transmission and incidence of Cucurbit yellow stunting disorder virus. Pest management science. 2017;73(7):1462-1472.

9. Lapidot M, Legg JP, Wintermantel WM, Polston JE. Management of whitefly-transmitted viruses in open-field production systems. In Advances in virus research. 2014;90:147-206. Academic press.

10. Abou-Jawdah Y, Sobh H, Fayad A, Lecoq H, Delécolle B, Trad-Ferre J. Cucurbit yellow stunting disorder virus-a new threat to cucurbits in Lebanon. Journal of Plant Pathology. 2000;55-60.

11. Li Y, Mbata GN, Punnuri S, Simmons AM, Shapiro-Ilan DI. *Bemisia tabaci* on vegetables in the Southern United States: Incidence, impact, and management. Insects. 2021;12(3):198.

12. Kreuder Johnson C, Hitchens PL, Smiley Evans T, Goldstein T, Thomas K, Clements A, Mazet JK. Spillover and pandemic properties of zoonotic viruses with high host plasticity. Scientific reports. 2015;5(1):14830.

13. Gilbert M, Slingenbergh J, Xiao X. Climate change and avian influenza. Revue scientifique et technique (International Office of Epizootics). 2008; 27(2):459.

14. Ebert AW, Engels JM, Schafleitner R, Hintum TV, Mwila G. Critical review of the increasing complexity of access and benefit-sharing policies of genetic resources for genebank curators and plant breeders-a public and private sector perspective. Plants. 2023;12(16):2992.

15. Coutts RH, Livieratos IC. Nucleotide sequence and genome organization of Cucurbit yellow stunting disorder virus RNA1. Archives of virology. 2003;148: 2055-2062.

16. Kiss ZA, Medina V, Falk BW. Crinivirus replication and host interactions. Frontiers in Microbiology. 2013;4:99.

17. Liu HY, Wisler GC, Duffus JE. Particle lengths of whitefly-transmitted criniviruses. Plant Disease. 2000;84(7):803-805.
18. Rohaim MA, El Naggar RF, Clayton E, Munir M. Structural and functional insights into non-structural proteins of coronaviruses. Microbial pathogenesis. 2021;150:104641.

19. Sawicki SG, Sawicki DL, Siddell SG. A contemporary view of coronavirus transcription. Journal of virology. 2007;81(1):20-29.

20. Dolja VV, Kreuze JF, Valkonen JP. Comparative and functional genomics of closteroviruses. Virus research. 2006;117(1):38-51.

21. Das A, Singh S, Islam Z, Munshi AD, Behera TK, Dutta S, Dey SS. Current progress in genetic and genomics-aided breeding for stress resistance in cucumber (Cucumis sativus L.). Scientia Horticulturae. 2022;300:111059.

22. Wisler GC, Duffus JE, Liu HY, Li RH. Ecology and epidemiology of whitefly-transmitted closteroviruses. Plant disease. 1998;82(3):270-280.

23. May FJ, Davis CT, Tesh RB, Barrett AD. Phylogeography of West Nile virus: From the cradle of evolution in Africa to Eurasia, Australia, and the Americas. Journal of virology. 2011;85(6):2964-2974.

24. May FJ, Davis CT, Tesh RB, Barrett AD. Phylogeography of West Nile virus: from the cradle of evolution in Africa to Eurasia, Australia, and the Americas. Journal of virology. 2011;85(6):2964-2974.

25. Castle S, Palumbo J, Prabhaker N. Newer insecticides for plant virus disease management. Virus Research. 2009;141(2):131-139.

26. Lapidot M, Legg JP, Wintermantel WM, Polston JE. Management of whitefly-transmitted viruses in open-field production systems. In Advances in virus research 2014;90:147-206. Academic press.

27. Patz JA, Graczyk TK, Geller N, Vittor AY. Effects of environmental change on emerging parasitic diseases. International Journal for Parasitology. 2000;30(12-13):1395-1405.

28. Dolja VV, Kreuze JF, Valkonen JP. Comparative and functional genomics of closteroviruses. Virus research. 2006;117(1):38-51.

29. Dolja VV, Kreuze JF, Valkonen JP. Comparative and functional genomics of closteroviruses. Virus research. 2006; 117(1):38-51.

30. Wintermantel WM, Wisler GC, Anchieta AG, Liu HY, Karasev AV, Tzanetakis IE. The complete nucleotide sequence and genome organization of Tomato chlorosis virus. Archives of virology. 2005;150:2287-2298.

31. Wisler GC, Li RH, Liu HY, Lowry DS, Duffus JE. Tomato chlorosis virus: A new whitefly-transmitted, phloem-limited, bipartite closterovirus of tomato. Phytopathology. 1998;88(5):402-409.

32. Halbert DN, Cutt JR, Shenk T. Adenovirus early region 4 encodes functions required for efficient DNA replication, late gene expression, and host cell shutoff. Journal of Virology. 1985;56(1):250.

33. Navarro JA, Sanchez-Navarro JA, Pallas V. Key checkpoints in the movement of plant viruses through the host. Advances in virus research. 2019;104:1-64.

34. Van Dorst HJM, Peters D. Some biological observations on pale fruit, a viroid-incited disease of cucumber. Netherlands Journal of Plant Pathology. 1974;80: 85-96.

35. Dale JL. Banana bunchy top: An economically important tropical plant virus disease. Advances in virus research. 1987;33:301-325.

36. Marco CF, Aguilar JM, Abad J, Gómez-Guillamón ML, Aranda MA. Melon resistance to Cucurbit yellow stunting disorder virus is characterized by reduced virus accumulation. Phytopathology. 2003;93(7): 844-852.

37. Castle S, Palumbo J, Merten P, Cowden C, Prabhaker N. Effects of foliar and systemic insecticides on whitefly transmission and incidence of Cucurbit yellow stunting disorder virus. Pest management science. 2017;73(7):1462-1472.

38. Castle S, Palumbo J, Merten P, Cowden C, Prabhaker N. Effects of foliar and systemic insecticides on whitefly transmission and incidence of Cucurbit yellow stunting disorder virus. Pest management science. 2017;73(7):1462-1472.

39. Abrahamian P, Sobh H, Seblani R, Abou-Jawdah Y. Co-infection of two criniviruses and a begomovirus enhances the disease severity in cucumber. European Journal of Plant Pathology. 2015;142:521-530.
40. LOOP AOART. Ghanem, Gamal Amin, kheder, Ahmed Abd El-Aziz 2, 3, Noura-Hassan, Mohamed, Abdel-Alim, Ahmed. International Journal. 2016;4(3):744-751.

41. Papayiannis LC, Ioannou N, Boubourakas IN, Dovas CI, Katis NI, Falk BW. Incidence of viruses infecting cucurbits in Cyprus. Journal of Phytopathology. 2005;153(9):530-535.

42. Berdiales B, Bernal JJ, Sáez E, Woudt B, Beitia F, Rodríguez-Cerezo E. Occurrence of cucurbit yellow stunting disorder virus (CYSDV) and beet pseudo-yellows virus in cucurbit crops in Spain and transmission of CYSDV by two biotypes of *Bemisia tabaci*. European Journal of Plant Pathology. 1999;105: 211-215.

43. Bhat AI, Aman R, Mahfouz M. Onsite detection of plant viruses using isothermal amplification assays. Plant biotechnology Journal. 2022;20(10):1859-1873.

44. Tollenaere C, Susi H, Laine AL. Evolutionary and epidemiological implications of multiple infection in plants. Trends in plant science. 2016;21(1):80-90.

45. Wintermantel WM. Emergence of greenhouse whitefly (Trialeurodes vaporariorum) transmitted criniviruses as threats to vegetable and fruit production in North America. APSnet Features; 2004.

46. De Paschale M, Clerici P. Serological diagnosis of Epstein-Barr virus infection: problems and solutions. World journal of virology. 2012;1(1):31.

47. Jeong JJ, Ju HJ, Noh J. A review of detection methods for the plant viruses. Research in Plant Disease. 2014;20(3):173-181.

48. Karasev AV. Genetic diversity and evolution of closteroviruses. Annual review of phytopathology. 2000;38(1):293-324.

49. Gilbertson RL, Rojas M, Natwick E. Development of integrated pest management (IPM) strategies for whitefly (*Bemisia tabaci*)-transmissible geminiviruses. In *The Whitefly, Bemisia tabaci* (Homoptera: Aleyrodidae) Interaction with Geminivirus-Infected Host Plants: Bemisia tabaci, Host Plants and Geminiviruses*. Dordrecht: Springer Netherlands. 2011;323-356.

50. Seal D, Zhang S, Ozores-Hampton M, Dittmar P, Li Y, Klassen W, Olczyk T. Summer squash production in Miami-Dade

County, Florida. University of Florida; 2006. IFAS Extension, # HS-861. Available: http://edis. ifas. ufl. edu/tr012 [25 May 2016].

51. Seal D, Zhang S, Dittmar P, Li Y, Klassen W, Wang Q, Olczyk T. Summer Squash Production in Miami-Dade County, Florida: HS-861/TR012, rev. 6/2022. EDIS. 2022;4:16-16.

52. Lapidot M, Legg JP, Wintermantel WM, Polston JE. Management of whitefly-transmitted viruses in open-field production systems. In Advances in virus research 2014;90:147-206. Academic press.

53. Castle S, Palumbo J, Prabhaker N. Newer insecticides for plant virus disease management. Virus Research. 2009;141(2):131-139.

54. Radouane N, Ezrari S, Belabess Z, Tahiri A, Tahzima R, Massart S, Lahlali R. Viruses of cucurbit crops: Current status in the Mediterranean Region. Phytopathologia Mediterranea. 2021;60(3).

55. McGrath MT. Diseases of cucurbits and their management. Diseases of fruits and vegetables. 2004;1:455-510.

56. Abrahamian PE, Abou-Jawdah Y. Whitefly-transmitted criniviruses of cucurbits: Current status and future prospects. Virusdisease. 2014;25:26-38.

57. Lapidot M, Legg JP, Wintermantel WM, Polston JE. Management of whitefly-transmitted viruses in open-field production systems. In Advances in virus research 2014;90:147-206. Academic press.

58. Messelink GJ, Calvo FJ, Marín F, Janssen D. Cucurbits. Integrated Pest and Disease Management in Greenhouse Crops. 2020;537-566.

59. Stansly PA, Natwick ET. Integrated systems for managing *Bemisia tabaci* in protected and open field agriculture. *Bemisia*: Bionomics and management of a global pest. 2010;467-497.

60. Alcaide C, Rabadán MP, Moreno-Perez MG, Gómez P. Implications of mixed viral infections on plant disease ecology and evolution. Advances in virus research. 2020;106:145-169.

61. Moriones E, Navas-Castillo J. Tomato yellow leaf curl virus, an emerging virus complex causing epidemics
worldwide. Virus research. 2000;71(1-2):123-134.

62. López-Sesé Al, Gómez-Guillamón ML. Resistance to Cucurbit yellowing stunting disorder virus (CYSDV) in Cucumis melo L. *HortScience*. 2000;35(1): 110-113.

63. Adkins S, Webster CG, Kousik CS, Webb SE, Roberts PD, Stansly PA, Turechek WW. Ecology and management of whitefly-transmitted viruses of vegetable crops in Florida. Virus Research. 2011;159(2):110-114.

64. Gil-Salas FM, Morris J, Cuadrado IM, Budge G, Colyer A, Boonham N, Janssen D. Risk assessment procedure for Cucumber vein yellowing virus (CVYV) and Cucurbit yellow stunting disorder virus (CYSDV) using a real time RT-PCR assay-study of virus levels in the vector *Bemisia tabaci*. In International Symposium on Vegetable Production, Quality and Process Standardization in Chain: a Worldwide Perspective. 2008;944:59-62.

65. Ng JC, Zhou JS. Insect vector-plant virus interactions associated with non-circulative, semi-persistent transmission: Current perspectives and future challenges. Current opinion in virology. 2015;15:48-55.

66. Chikh-Rouhou H, Abdedayem W, Solmaz I, Sari N, Garcés-Claver A. Melon (Cucumis melo L.): Genomics and Breeding. In Smart Plant Breeding for Vegetable Crops in Post-genomics Era. Singapore: Springer Nature Singapore. 2023;25-52

67. Castle S, Palumbo J, Prabhaker N. Newer insecticides for plant virus disease management. Virus Research. 2009;141(2):131-139.

68. McGrath MT. Diseases of cucurbits and their management. Diseases of fruits and vegetables. 2004;1:455-510.

69. Lapidot M, Legg JP, Wintermantel WM, Polston JE. Management of whitefly-transmitted viruses in open-field production systems. In Advances in virus research 2014;90:147-206. Academic press.

70. Castle S, Palumbo J, Merten P, Cowden C, Prabhaker N. Effects of foliar and systemic insecticides on whitefly transmission and incidence of Cucurbit yellow stunting disorder virus. Pest management science. 2017;73(7):1462-1472.

71. Carrière Y, Degain B, Hartfield KA, Nolte KD, Marsh SE, Ellers-Kirk C, Palumbo JC. Assessing transmission of crop diseases by insect vectors in a landscape context. Journal of economic entomology. 2014;107(1):1-10.

72. Martín-Hernández AM, Picó B. Natural resistances to viruses in cucurbits. Agronomy. 2020;11(1):23.

73. Velasco L, Ruiz L, Galipienso L, Rubio L, Janssen D. A historical account of viruses in intensive horticultural crops in the Spanish mediterranean arc: New challenges for a sustainable agriculture. Agronomy. 2020;10(6):860.

74. Lapidot M, Legg JP, Wintermantel WM, Polston JE. Management of whitefly-transmitted viruses in open-field production systems. In Advances in virus research. 2014;90:147-206. Academic press.

75. Romay G, Lecoq H, Desbiez C. Cucurbit crops and their viral diseases in Latin America and the Caribbean islands: A review. Journal of Plant Pathology. 2014;96(2).

76. Li Y, Mbata GN, Punnuri S, Simmons AM, Shapiro-Ilan DI. *Bemisia tabaci* on vegetables in the Southern United States: Incidence, impact, and management. Insects. 2021;12(3):198.

77. Lecoq H, Desbiez C. Viruses of cucurbit crops in the Mediterranean region: An ever-changing picture. In Advances in virus research. 2012;84:67-126. Academic Press.

78. Pitrat M. Vegetable crops in the mediterranean basin with an overview of virus resistance. Advances in Virus Research. 2012;84:1-29.

79. EFSA Panel on Plant Health (PLH). Scientific opinion on the risks to plant health posed by *Bemisia tabaci* species complex and viruses it transmits for the EU territory. EFSA journal. 2013;11(4):3162.

80. Kaur N, Chen W, Fei Z, Wintermantel WM. Differences in gene expression in whitefly associated with CYSDV-infected and virus-free melon, and comparison with expression in whiteflies fed on ToCV-and TYLCV-infected tomato. BMC genomics. 2019;20:1-18.

81. Lecoq H, Katis N. Control of cucurbit viruses. In Advances in virus research. 2014;90:255-296. Academic Press.

82. Gilbertson RL, Rojas M, Natwick E. Development of integrated pest
management (IPM) strategies for whitefly
(Bemisia tabaci)-transmissible
geminiviruses. In The Whitefly, Bemisia
tabaci (Homoptera: Aleyrodidae)

Interaction with Geminivirus-Infected Host
Plants: Bemisia tabaci, Host Plants and
Geminiviruses. Dordrecht: Springer
Netherlands. 2011;323-356.

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