# A stage-dependent seed defense response to explain efficient seed transmission of Xanthomonas citri pv. fuscans to common bean

Armelle Darrasse, Łukasz Pawel Tarkowski, Martial Briand, David Lalanne, Nicolas Chen, Matthieu Barret, Jerome Verdier

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## To cite this version:

Armelle Darrasse, Łukasz Pawel Tarkowski, Martial Briand, David Lalanne, Nicolas Chen, et al.. A stage-dependent seed defense response to explain efficient seed transmission of Xanthomonas citri pv. fuscans to common bean. Plant, Cell and Environment, 2024, 10.1111/pce.15037. hal-04665509

HAL Id: hal-04665509

https://hal.inrae.fr/hal-04665509v1

Submitted on 31 Jul 2024

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Distributed under a Creative Commons Attribution 4.0 International License
A "ceasefire" model to explain efficient seed transmission of
Xanthomonas citri pv. fuscans to common bean.

<table><tr><td>Journal:</td><td>Plant, Cell & Environment</td></tr><tr><td>Manuscript ID</td><td>PCE-23-1399</td></tr><tr><td>Wiley - Manuscript type:</td><td>Original Article</td></tr><tr><td>Date Submitted by the Author:</td><td>05-Dec-2023</td></tr><tr><td>Complete List of Authors:</td><td>Verdier, Jerome; Institut de Recherche en Horticulture et Semences Darrasse, Armelle; Institut de Recherche en Horticulture et Semences Tarkowski, Łukasz Paweł; Institut de Recherche en Horticulture et Semences Briand, Martial; Institut de Recherche en Horticulture et Semences Lalanne, David; Institut de Recherche en Horticulture et Semences Chen, Nicolas W.G.: Institut de Recherche en Horticulture et Semences barret, matthieu; Institut de Recherche en Horticulture et Semences</td></tr><tr><td>Suggested Editor:</td><td>Corne Pieterse</td></tr><tr><td>Environment Keywords:</td><td>pathogens</td></tr><tr><td>Physiology Keywords:</td><td>development</td></tr><tr><td>Other Keywords:</td><td>Seed, &lt;i&gt;Xanthomonas&lt;/i&gt;, transmission, &lt;i&gt;Phaseolus vulgaris&lt;/i&gt;</td></tr><tr><td>Abstract:</td><td>Although seed represents an important means of plant pathogen dispersion, the seed-pathogen dialogue remains largely unexplored. A multi-omic approach (i.e. dual RNAseq, plant small RNAs and methylome) was performed at different seed developmental stages of common bean (Phaseolus vulgaris L.) during asymptomatic colonization by Xanthomonas citri pv. fuscans (Xcf). In this condition, Xcf did not produce disease symptoms, neither affect seed development. Although, an intense molecular dialogue, via important transcriptional changes, was observed at the early seed developmental stages with down-regulation of plant defense signal transduction, via action of plant miR, and upregulation of the bacterial Type 3 Secretion System. At later seed maturation stages, molecular dialogue between host and pathogen was reduced to few transcriptome changes, but marked by changes in DNA methylation of plant defense and germination genes, in response to Xcf colonization, potentially acting as defense priming to prepare the host for the post-germination battle. This distinct response of infected seeds during maturation, with a more active role at early stages refutes the widely diffused assumption considering seeds as passive carriers of microbes. Finally, our data support a novel plant-pathogen interaction model, specific to the seed tissues, which differs from others by the existence of distinct phases during seed-pathogen interaction with seeds first actively interacting with colonizing pathogens, then both belligerents switch to more passive mode at later stages. We contextualized this</td></tr></table>
<table><tr><td></td><td>observed scenario in a novel hypothetical model that we called “ceasefire”, where both the pathogen and the host benefit from temporarily laying down their weapons until the moment of germination.</td></tr></table>

Note: The following files were submitted by the author for peer review, but cannot be converted to PDF. You must view these files (e.g. movies) online.

Supplementary Data S1.xlsx

Supplementary Data S3.xlsx
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1 A "ceasefire" model to explain efficient seed transmission of *Xanthomonas citri* pv. *fuscans* to common bean.

2 fuscans to common bean.

3

4 Armelle Darrasse, Łukasz Paweł Tarkowski, Martial Briand, David Lalanne, Nicolas W.G.

5 Chen, Matthieu Barret, Jerome Verdier

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7 Univ Angers, Institut Agro, INRAE, IRHS, SFR QUASAV, F-49000 Angers, France

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9 Author for correspondence: Jerome Verdier

10 jerome.verdier@inrae.fr

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ORCIDs

13 Armelle Darrasse: 0000-0002-9334-5862

14 Łukasz Paweł Tarkowski: 0000-0002-5419-6606

15 Nicolas W.G. Chen: 0000-0002-7528-4656

16 Matthieu Barret: 0000-0002-7633-8476

17 Jerome Verdier: 0000-0003-3039-2159

18

19 Indicate the number of words (Full papers <6,500 words): 6,425

20 Indicate the number of figures: 4 figures (all in color), 2 tables, 4 supplementary data

21

Summary

* Although seed represents an important means of plant pathogen dispersion, the seed-pathogen dialogue remains largely unexplored.

* A multi-omic approach (i.e. dual RNAseq, plant small RNAs and methylome) was performed at different seed developmental stages of common bean (*Phaseolus vulgaris* L.) during asymptomatic colonization by *Xanthomonas citri* pv. *fuscans* (*Xcf*).

* In this condition, *Xcf* did not produce disease symptoms, neither affect seed development. Although, an intense molecular dialogue, via important transcriptional changes, was observed at the early seed developmental stages with down-regulation of plant defense signal transduction, via action of plant miR, and upregulation of the bacterial Type 3 Secretion System. At later seed maturation stages, molecular dialogue between host and pathogen was reduced to few transcriptome changes, but marked by changes in DNA methylation of plant defense and germination genes, in response to *Xcf* colonization, potentially acting as defense priming to prepare the host for the post-germination battle. This distinct response of infected seeds during
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maturation, with a more active role at early stages refutes the widely diffused assumption considering seeds as passive carriers of microbes.

* Finally, our data support a novel plant-pathogen interaction model, specific to the seed tissues, which differs from others by the existence of distinct phases during seed-pathogen interaction with seeds first actively interacting with colonizing pathogens, then both belligerents switch to more passive mode at later stages. We contextualized this observed scenario in a novel hypothetical model that we called "ceasefire", where both the pathogen and the host benefit from temporarily laying down their weapons until the moment of germination.

**Key words:** Seed, *Xanthomonas*, transmission, dialogue, epigenome, *Phaseolus vulgaris*, dual transcriptomics.

## Introduction

Legumes provide a sustainable source of proteins for human and livestock diet, moreover their symbiotic nitrogen fixation capacity contributes to soil preservation and reduces the need for chemical fertilizers (Stagnari et al., 2017; Ferreira et al., 2021). An important factor limiting legume utilization is their relatively high yield variability, greatly due to their susceptibility to environmental factors such as biotic and abiotic stresses (Cernay et al., 2015; Martins et al., 2020). While legumes are expected to better perform under changing climatic conditions in relation to other crops thanks to higher biomass accumulation under increased atmospheric CO₂ levels and higher photosynthetic efficiency under increased irradiation levels, other traits are predicted to be negatively affected, such as seed quality and resistance to pathogens (Myers et al., 2014).

Pathogens are responsible for 35-70% yield losses on grain legumes (Martins et al., 2020). An important determinant of disease outbreak is pathogen dispersal through infected seeds (Denancé and Grimault, 2022). The mode of transmission of pathogens to the seed can be schematically summarized in three non-exclusive pathways: internal (*via* the host xylem), floral (*via* the pistil) and external as a consequence of contact of the seed with symptomatic fruit tissues or with threshing residues (Maude, 1996). For instance, *Xanthomonas citri* pv. *fuscans* (*Xcf*), causal agent of common bacterial blight of bean (CBB), can use these three pathways for its transmission to common bean seeds (Darsonval et al., 2008; Darrasse et al., 2018). Contaminated seeds can be symptomatic or asymptomatic, and are generally associated with high or moderate bacterial population sizes, respectively, moreover symptomatic seeds often fail to germinate (Darrasse et al. 2018; Chen et al., 2021a) and no viable pathogen control method to counteract bacterial seed infections exists.
Decades of research led to a comprehensive overview of the genetic (for review Dodds and Rathjen, 2010; Wirthmueller et al., 2013) and epigenetic (for review Hannan Parker et al., 2022) mechanisms involved in plant-pathogen interactions during vegetative growth. However, the molecular dialogue that takes place between seeds and pathogens was overlooked to date. On the plant side, in the event of an incompatible interaction between *Medicago truncatula* and *X. campestris* pv. *campestris* (Xcc), seed transcriptome exhibited an activation of defense response and a repression of seed maturation pathways (Terrasson et al., 2015). From the bacterial side, some specific genetic determinants such as the type 3 secretion system (T3SS, Darsonval et al., 2008) and adhesins (Darsonval et al., 2009) were shown to be involved in the transmission of Xcf to common bean seeds. Involvement of the T3SS in seed transmission was also documented for *Acidovorax citrulli* in watermelon (Dutta et al., 2014). However, a global view of bacterial transcriptomic changes occurring during seed transmission is currently missing. This lack of knowledge is partly due to the difficulties of collecting enough bacterial RNA from the seeds. Indeed seed-associated bacterial population sizes are usually very low (from 10 to 1,000 CFU per bean seed; Chesneau et al., 2022) and follow a Poisson distribution, which complicates the sampling of contaminated seeds and prevent molecular analysis of seed-pathogens interactions (Gitaitis and Walcott, 2007).

Since knowledge regarding molecular interactions occurring during bacterial seed infections is currently lacking, the objective of this work was to decipher the molecular dialogue between the common bean (*Phaseolus vulgaris* L.) seed and a seed pathogen at several stages of seed development in order to identify major molecular factors involved in seed infection establishment and pathogen transmission to the seedling. A dual RNAseq approach to identify both the host seed and the Xcf pathogen transcriptomes was performed at three stages of seed development during seed filling, seed maturation and seed maturity. The technical limitation of low bacterial population within seeds was successfully bypassed using bacterial transcript enrichment. This transcriptomic analysis was complemented by the analysis of small RNAs and DNA methylation changes in infected seeds to reveal the role of these mechanisms in the seed-pathogen interaction, which allowed us to propose a novel model in plant-pathogen interactions specific to seed developmental stage and explaining the efficiency of pathogen seed transmission.

# Materials and Methods

## Bacterial strain and inoculum preparation

The Xcf bacterial strain 7767R (RifR, Darrasse et al., 2018) was grown for 24h at 28°C in Tryptic Soy Agar at 10% (1.7 g.L⁻¹ tryptone, 0.3 g.L⁻¹ soybean peptone, 0.25 g.L⁻¹ glucose, 0.5 g.L⁻¹ NaCl, 0.5 g.L⁻¹ K₂HPO₄ and 15 g.L⁻¹ agar) supplemented with 50 mg.L⁻¹ rifamycin.
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Bacterial cells were suspended in sterile distilled water, calibrated at $10^8$ CFU.mL$^{-1}$ ($OD_{600}$ = 0.1) and adjusted to $10^6$ CFU.mL$^{-1}$ for spray-inoculation.

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## Plant materials and production of contaminated seeds

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Experiments were performed with *Phaseolus vulgaris* L. cv. Flavert, a cultivar susceptible to CBB (Darrasse et al., 2007). Seeds were sown in one liter of Tray substrate (NF U 44–551, Klasmann- Deilmann GmbH, Rippert France). Plants were grown in a controlled growth chamber with 16h of light at 23°C and 8h of dark at 20°C and a relative humidity (RH) of 70%. Plants were watered twice a week during the first three weeks, then with a nutrient solution (N/P/K=15/10/30). Plants were staked and pinched after the third leaf.

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Plants were spray-inoculated at the flower bud stage (R5, Michael 1994) with either Xcf bacterial suspension ($10^6$ CFU.mL$^{-1}$) or water as control. The day prior to inoculation, temperature (day 25°C/night 23°C) and RH (95%) were increased. Inoculation was performed using a two-step protocol. First, small green flower buds were sprayed. Three days later, flower buds at the pollination stage were tagged. Then, a second inoculation was performed at one day after pollination (DAP) when tagged organs turned into open flowers. Then afterward, RH was reduced to 70% to limit pathogen symptom development and seed abortion. Three independent replicates of five plants ($n=15$) were inoculated. Tagged pods were harvested at 24, 35 and 42 DAP. Seeds were collected aseptically from pods to avoid contamination by external bacterial populations (Darsonval et al., 2008).

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## Monitoring of bacterial population sizes

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For each sample, Xcf population sizes were determined from ten individual seeds and from five pools of three seeds. Seeds were soaked in 0.5 mL of sterile water per seed overnight at 4°C under shaking (150 rpm). Then, 50 µL of serial dilutions were plated on 10% TSA. Colonies were monitored five days after incubation at 28°C. The contamination rate of a sample (p) was calculated from the analysis of N sub-samples according to the formula $p = 1 - (Y/N)^{1/n}$ (Maury et al., 1985), where n is the number of seeds in each group and Y the number of healthy groups.

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## Seed physiological analyses

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Three sub-samples of ten seeds were used to determine dry weight and water content. Each sub-sample was weighed before and after incubation (3 days) in a 96°C incubator (Memmert).

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## Plant and bacterial RNA extraction and RNA sequencing

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Seed samples harvested at 24, 35 and 42 DAP were flash-frozen in liquid nitrogen. Samples were ground in liquid nitrogen using a mechanical grinder (Retsch MM300
TissueLyser) during 1 min at 30 Hertz. Total RNAs were extracted using the NucleoSpin® RNA Plant and Fungi Kit (Macherey-Nagel, Düren, Germany), according to the manufacturer instructions. RNA quantity and integrity were assessed respectively using a NanoDrop ND-1000 (NanoDrop Technologies, Wilmington, DE, USA) and a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Library constructions and single-end sequencing (SE50, 20M) were outsourced to the Beijing Genomics Institute (BGI, https://www.bgi.com) using the Illumina Hiseq 2500 technology. Raw reads are available at GSE226918.

Using the same seed lots as for plant RNAs, bacterial macerates were collected after soaking contaminated seeds (2 mL per gram of seed) overnight in KPO₄ buffer, (50 mM, pH 6.8), supplemented with 20% of blocking agent (RNAlater, Thermofisher scientific, Carlsbad, CA, United States). After centrifugation (15 min at 15,000 g) and removal of the supernatant, total RNAs were extracted as previously described (Darsonval et al., 2009). Concentration and integrity of RNAs were assessed with Qubit (Invitrogen, Carlsbad, CA, USA) and a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), respectively. As total RNA extracted from bacterial macerates corresponded mainly to plant transcripts (not shown), we designed a procedure of bacterial transcript enrichment. Bacterial mRNAs were captured using the SureSelectXT RNA Direct technology (Agilent, Santa Clara, CA, USA). A total of 54,548 probes of 120-nts length were designed based on the predicted mRNAs of Xcf7767R genome sequence (GCA_900234465; Chen et al., 2018). Quality and quantity of sequencing libraries were evaluated and quantified using Bioanalyzer and KAPA Library Quantification assay (Roche, Basel Switzerland). Paired-end sequencing (2 × 75 bp) was performed with a NextSeq 550 System High OutPut kit (Illumina, San Diego, CA, USA). Raw reads are available at GSE227386.

After quality control, high-quality reads were mapped either on Xcf 7767R transcriptome (Briand et al., 2021) (https://bbric-pipelines.toulouse.inra.fr/myGenomeBrowser?browse=1&portalname=Xcf7767Rpb&owner=armelle.darrasse@inrae.fr&key=TwzQ08DA) or on *P. vulgaris* transcriptome version 2.1 (https://phytozome-next.jgi.doe.gov/info/Pvulgaris_v2_1) using quasi-mapping alignment and quantification methods of Salmon algorithm v.1.2 (Patro et al., 2017). RNA-Seq data were normalized as transcripts per million (TPM). Differentially expressed genes (DEGs) were determined using DESeq2 v1.22.2 (Love et al., 2014), using an adjusted p-value <5%. Xcf DEGs were analyzed between sampling dates. *P. vulgaris* DEGs were obtained by comparing Xcf- versus H₂O-inoculated seeds at each developmental stage. Gene annotations were provided with the *P. vulgaris* version 2.1 genome and Mapman functional categories v.4 were determined using Mercator tool from the predicted protein sequences (Schwacke et al., 2019). Bacterial Type III effectors were predicted using the automated machine-learning based web
H₂O-inoculated seeds at 24 and 42 DAP were determined using DESeq2 following a *p*-value threshold < 5% from the SARTools R package (Varet et al., 2016). Known and putative novel small RNAs were mapped to the *P. vulgaris* genome sequence using ShortStack4 algorithm (Johnson et al., 2016) and displayed in the dedicated Jbrowse <https://iris.angers.inrae.fr/pvulgaris_v2> in the 'small RNA tracks' section. Transcripts potentially targeted by miRNAs were predicted via analyzing complementary matching between sRNA and target and evaluating target site accessibility using psRNATarget tool (Dai and Zhao, 2011; Dai et al., 2018) and a threshold of expectation below 5 was set to consider transcripts as putative miRNA targets. Raw reads are publicly available at GSE226920.

## Plant DNA extraction and Bisulfite sequencing experiments (BS-seq)

From the same frozen seed powders used for RNA extractions, we performed DNA extraction, on the three biological replicates of *Xcf*- and H₂O-inoculated seeds at 42 DAP, using the NucleoSpin® DNA Food Kit (Macherey-Nagel, Düren, Germany), according to the manufacturer's instructions. DNA samples were sent to the BGI Genomics (Hong Kong) for bisulfite treatment using a ZYMO EZ DNA Methylation-Gold kit, library construction and paired-end sequencing using BGISEQ-500 sequencing technology (PE100 45M). FastQC was used to check sequencing quality and clean reads were mapped to the *P. vulgaris* genome version 2.1 using Bismark software (Krueger and Andrews, 2011). After mapping, deduplication of sequences and quantification of cytosine methylation were performed using Bismark_deduplicate and Bismark_methylation extractor. Each context of methylation was considered independently: CG, CHG, or CHH and corresponding bigwig files were generated using bismark_to_bigwig python script and displayed in the dedicated Jbrowse: <https://iris.angers.inrae.fr/pvulgaris_v2>. Putative differentially methylated regions (DMRs) were identified in each independent methylation context using DMRCaller algorithm available in R (Catoni et al., 2018). Raw reads are publicly available at <https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE226919>.

## Results

*Seed transmission of moderate *Xcf* population sizes does not impact seed development*

Seed transmission of *Xcf* 7767R was investigated following spray-inoculation of *P. vulgaris* L. cv Flavert. Three stages of seed development were targeted: (i) 24 DAP (seed filling), (ii) 35 DAP (seed maturation) and 42 DAP (seed maturity). Seed water content (Fig. 1A) and dry seed weight (Fig. 1B) were not significantly impacted by *Xcf*-inoculation. As
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3 254 described in Darsonval et al. (2008), we used 10⁶ CFU.mL⁻¹ for Xcf spray-inoculation at
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5 255 flowering time to allow seed bacterial transmission without apparition of symptoms during seed
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6 256 development. Otherwise, higher concentration could generate symptomatic seed bacterial
7 257 transmission leading to defect in germination of infected seeds. Following this mild treatment,
8 258 about 80% of seeds were contaminated with Xcf with an average population size of 10⁵ CFU.g⁻¹
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10 259 of seeds at 24 DAP (Fig. 1C). Over the course of seed development, the frequency of
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12 260 detection of Xcf decreased from 80% to 50%. This was accompanied by a significant decrease
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14 261 in Xcf population size from 35 to 42 DAP, down to an average of 10³ CFU.g⁻¹ of seeds at
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16 262 maturity (Fig. 1C).

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263 **Changes in the Xcf bacterial transcriptome during seed development**

264 To explore the genetic determinants involved in Xcf seed transmission, dual (host and pathogen) transcriptome sequencing was performed at 24, 35 and 42 DAP. An essential step to obtain sufficient bacterial transcript data was to enrich RNA-Seq libraries for Xcf transcripts using 54,656 capture-probes. Among a total of 27.7 to 61.3 M sequenced reads that were obtained for each sample, 4.7 to 55.1 M mapped on the predicted transcriptome of Xcf strain 7767R (Supplementary Table S1). A total of 4,372 mRNA were detected in at least one sample (count ≥10), which corresponded to >96% of the 4,537 predicted mRNA, thus validating our Xcf transcriptome enrichment strategy. Extensive changes in Xcf transcriptome were observed between seed filling (24 DAP) and the two other seed maturation stages (35 and 42 DAP). Indeed, 865 and 1,674 DEGs were detected between 24 and 35 DAP and 24 and 42 DAP, respectively, (Fig. 2A). On the other hand, only 17 DEGs were detected between 35 and 42 DAP, indicating that transcriptomic levels stabilized between seed maturation and maturity stages. In line with this result, over-representation analyses of COG terms associated to bacterial DEGs were performed and revealed that intracellular trafficking and secretion terms were enriched at 24 DAP and post-translational modification at 35 and 42 DAP (Fig. 2B). The other enriched categories were translation and reparation/ repair, both enriched at 42 DAP, and extracellular structure and cell motility, both enriched at 24 DAP (Fig. 2B).

281 A focus on the COG related to secretion processes revealed that all T3SS encoding
282 genes and several xps genes involved in the T2SS were up-regulated at the seed filling stage,
283 but not later during seed maturation (Supplementary Table S1). This was consistent with the
284 observed up-regulation of the master regulator hrpG that is known to control many genes
285 involved in the interaction with the host plant (Teper et al., 2021) such as the T3SS
286 transcriptional activator hrpX and cognate effectors (T3Es) but also the xps genes involved in
287 the secretion of cell wall degrading enzymes (Szczesny et al., 2010). In line with this result,
288 26/40 (65%) of T3E-encoding genes and several genes encoding pectin lyase (1), pectate
lyases (2), glycoside hydrolases (34) and proteases (40) were only up-regulated at early stage (Supplementary Table S1). As demonstrated in Darsonval et al. (2008), T3SS encoding genes, including hrpG and hrpX are essential genes for efficient bacterial seed transmission. Together, these results suggested that bacteria were actively interacting with the host plant only at early seed maturation stages, but not later.

## Transcriptomic analysis of bean seeds in response to pathogen colonization

Changes in *P. vulgaris* transcriptome were assessed using the same seed lots as for the Xcf transcriptome analyses described above. All results from this RNA-Seq analysis are displayed in the dedicated Jbrowse (https://iris.angers.inrae.fr/pvulgaris_v2) and in Supplementary Table S1. Similar to what was observed in Xcf transcriptome changes, RNA-Seq analysis revealed that the plant response to the bacteria was higher at early than later stages of seed maturation, with 1,826 DEGs at 24 DAP, 1,351 at 35 DAP and only 105 at 42 DAP (Fig. 2C). Only 137 DEGs (7.5% of 24 DAP DEGs) were shared between 24 and 35 DAP, indicating that the plant's response was different between these stages, ending up with almost no response in mature seeds. Only one DEG, encoding a CHAPERONE PROTEIN DNAJ-LIKE PROTEIN, was found to be in common between all the three stages (*Phvul.001G262000*) and could reflect a cellular stress in seeds inoculated with Xcf. This low overlap in DEGs across different seed developmental stages was also reflected at the level of functional category enrichments, which were different between 24, 35 and 42 DAP (Fig. 2D). The 24 DAP timepoint displayed the most complex response, with six up-regulated and nine down-regulated Mapman functional categories detected through functional enrichment analysis of DEGs. Some categories had well characterized roles in the plant-microbe molecular dialogue, such as Leucine Rich Repeat protein kinases (LRRs), which were up-regulated in Xcf-inoculated seeds (i.e. up-regulation of 15 annotated LRR related proteins), whereas the Mitogen-Activated Protein Kinases (MAPKs) and transcription factors (TF) of the bZIP, TIFY and AP2/ERF classes were down-regulated. At 24 DAP, in parallel to the down-regulation of MAPKs known to be involved in defense signal transduction such as MAPKKK3, MAPK3 or MAPK4, we also identified down-regulation of defense related genes such as two encoding thaumatin pathogenesis-related (PR) proteins, five JAZ and one JAR genes involved in the jasmonic acid pathway, but also PAD4, a central regulator of the salicylic acid pathway (Supplementary Table S1). At 35 DAP, functional ontology enrichment detected four up-regulated categories related to peptidase/protease activities and transfer of carbon skeletons. At 42 DAP, only two up-regulated categories (chromatin regulation and calcium-permeable channel) were detected.

## Small RNAs associated with Xcf seed colonization
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To further characterize the molecular dialogue between the colonized seeds and Xcf and the changes in plant transcript expression we focused our analysis on small RNA changes between colonized and healthy seeds at two contrasted stages, at 24 DAP to decipher if transcriptome changes due to plant response to pathogen could be mediated by small RNAs and at 42 DAP to reveal if specific small RNA could be stored at seed maturity to mediate defense response at post-germinative stage. Following sequencing and mapping against the mature miRNA database (miRBase release 22), we observed a total of 255 and 112 mature miRNAs differentially expressed (p< 0.05) between Xcf-colonized and healthy seeds at 24 and 42 DAP, respectively. At 24 DAP, mature miRNA up-regulated in Xcf-colonized seeds belonged to six miRNA families (miR162, miR172, miR396, miR482, miR6478 and miR8175), while four miRNA families showed down-regulation (let7, miR21, miR2111 and miR482) (Supplementary Table S2, Table 1). Similarly, at 42 DAP, we observed up-regulation of only one miRNA family (miR31) and down-regulation of two miRNA families (miR164 and miR451) (Supplementary Table S2, table 1). These data further confirmed that the molecular dialogue was more intense at early stages compared to later stages. Moreover, several miRNA families differentially regulated in Xcf-inoculated seeds were known to be involved in plant defense response such as miR482 (Shivaprasad et al., 2012), miR396 (Soto-Suárez et al., 2017) and miR172 (Holt et al., 2015). Known and unknown identified small RNAs were mapped to the genome using ShortStack version 4 and are available in the dedicated *P. vulgaris* Jbrowse (https://iris.angers.inrae.fr/pvulgaris_v2).

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To reveal the potential response mediated by these miRNAs, we identified putative transcript targets using (i) psRNATarget predictive tool (Dai et al. 2018) combined with (ii) our generated transcriptomic data at these two stages (Supplementary Table S2). To clarify, a transcript was considered as putative miRNA target if (i) its expectation (E) score from PsRNATarget was below 5 and if (ii) its expression was down-regulated when miRNA was up-regulated or inversely. Following these criteria, we identified between one to 11 putative miRNA target transcripts depending on miRNA families (Table 1). Among miRNAs up-regulated at 24 DAP in Xcf-inoculated, there were target transcripts related to defense such as miR8175 that could down-regulate key defense genes such as *PAD4-LIKE* involved in the defense pathway mediated by salicylic acid or more generic ones potentially involved in defense signaling such as a calcium-dependent-lipid-binding domain gene (CalB) or phospholipase A1 (Table 1). At the opposite, in the Xcf-inoculated seeds, we observed down-regulation of miRNA families such as let7, miR21, miR2111 and miR482 that potentially enhanced expression of developmental/growth genes such as TOR-LIKE, MED15, MED13, NOC1/SWA2. At 42 DAP, only three miR families, miR31, miR451 and miR164, showed significant expression changes between Xcf-infected and healthy seeds. An unique putative
transcript target was identified associated with miR451, which encodes a UBP26-LIKE protein potentially involved in the heterochromatin silencing at the end of the seed maturation (Luo et al., 2008). In conclusion, these results suggested that miRNA did mediate seed growth by silencing defense response at 24 DAP during early seed development. On the other hand at maturity, even if miR164 up-regulation was already shown to be involved in plant defense against fungi in cotton (Gossypium hirsutum) and Populus tomentosa (Hu et al., 2020; Chen et al., 2021b), in our susceptible host this miR was down-regulated at 42 DAP, which did not support the hypothesis that specific miRNA were accumulated in Xcf-inoculated seeds to prepare plant defense during germination. Interestingly, at 24 and 42 DAP, we observed that plant miRNA could support seed defense silencing probably due to the bacteria infection arsenal such as its T3Es activated early during seed development.

### Seed methylome dynamics associated with Xanthomonas seed colonization

To better understand the plant defense response and the impact of the bacterial colonization during seed development, we analyzed the changes in the seed methylomes of healthy and Xcf-colonized bean seeds at seed maturity (42 DAP). Indeed, DNA methylation was already described as a relevant mechanism in defense priming and plant immunity (for review see Deleris et al., 2016; Espinas et al., 2016). By focusing on the mature stage, we intended to capture the cumulative impact on DNA methylation of the bacterial colonization throughout seed development. The comparison of Xcf-colonized versus healthy seeds samples revealed 954 Differentially Methylated Regions (DMRs), of which 61.95% were hypomethylated (loss of methylation due to bacterial colonization) and 38.05% hypermethylated (gain of methylation due to bacterial colonization) (Supplementary Table S3). Not surprisingly, DMRs were predominantly localized on sequences containing transposable elements or repeats (74.1% of total DMRs), while 7.9% and 4.5% were located within gene and promoter sequences, respectively (Fig. 3A). Regarding the methylation context, we mainly observed DMRs in the CHH (i.e. 481 DMRs) and CHG (i.e. 394 DMRs) contexts, while only 79 were related to the CG context. The complete list of the differentially methylated genes can be found in Supplementary Table S3 and in the dedicated Jbrowse (https://iris.angers.inrae.fr/pvulgaris_v2).

We identified a total of 102 DMRs located within either coding (n=66) or promoter regions (n=36) of annotated genes, affecting 99 unique genes. Among coding sequences, 33 genes resulted in hypomethylation and 33 hypermethylation, while among promoter regions 27 genes were hypomethylated and 9 hypermethylated (Fig. 3B). To understand the role of genes differentially methylated in promoter and coding sequences at seed maturity, we compared with their changes in expression and did not observe any overlap with the DEGs
1
2
3 395 between Xcf-colonized and non-colonized mature seeds, suggesting that differentially
4
5 396 methylated regions did not regulate gene expression during seed development. To understand
6
7 397 the potential role of these DMRs in the host-pathogen interaction, we looked at genes involved
8 398 both in the germination and defense processes. First, from the dataset generated from Narsai
9
10 399 et al. (2017) during ten early stages of A. thaliana seed germination, we identified 21,015
11
12 400 genes showing a differential expression (adjusted pvalue <1% using ImpulseDE2) during
genesis
13 401 germination process, therefore potentially involved in germination. By mapping P. vulgaris
14
15 402 transcripts on Arabidopsis transcripts, we identified potential homologous transcripts in these
16
17 403 two species and revealed a statistically significant enrichment (Fig. 3B, Fisher's Exact test p-
18
19 404 value< 2.2e-16) of P. vulgaris genes displaying DMRs following pathogen colonization with
20
21 405 those differentially expressed during germination. Indeed, out of the 90 homologous genes
22
23 406 identified in A. thaliana and displaying DMR, 78 were genes differentially expressed during
germination
24
25 407 Second, by analyzing the list of 99 unique genes displaying changes in
26
27 408 methylation levels following bacterial colonization, we compiled a list of genes with putative
28
29 409 roles in defense. We identified 17 genes, 10 hypomethylated and 7 hypermethylated following
30 410 bacterial infection (Table 2). As example, we observed five LRR-related protein kinases, two
31
32 411 PR proteins, and some genes identified as involved in immune response such as PUB13-
33
34 412 LIKE, CES11-LIKE or WRKY72 (complete list in Supplementary Table S3). As it is known that
35
36 413 changes in the methylation state of transposable regions can also spread to adjacent regions
37
38 414 and regulate nearby gene expression (Ahmed et al., 2011), we extended our search to coding
39
40 415 sequences that are 5kb nearby DMRs located in transposable regions. This analysis detected
41 416 additional 280 genes potentially associated with DMRs located in transposable regions (61.4%
42 417 with hypomethylated regions and 38.6% with hypermethylated regions). Among these genes,
43
44 418 we observed a subgroup coding for disease resistance proteins, with 5 additional putative TIR-
45
46 419 NB-LRR proteins (Phvul.004G105600, Phvul.004G100300, Phvul.010G026400,
47
48 420 Phvul.010G027900, Phvul.010G028000), 3 putative NB-ARC proteins (Phvul.002G130300,
49 421 Phvul.002G130400, Phvul.004G076100) and 4 putative LRR kinases (Phvul.008G164500,
50
51 422 Phvul.008G164600, Phvul.005G162100, Phvul.005G162000) (Table 2, Supplementary Table
52
53 423 S3 and in the dedicated Jbrowse). In total, we listed 17 DMRs nearby genes associated with
defense processes (Table 2). A comparison between these two lists revealed that 5 genes
54
55 424 encoding three LRR related proteins (Phvul.008G164600, Phvul.005G162000 and
56
57 425 Phvul.005G163000), one TIR NBS LRR protein (Phvul.010G026400) and WRKY72 TF
58
59 426 (Phvul.003G068700), displayed DMRs both within their gene sequences and in transposable
elements located in proximal genomic regions. To define if these DMRs present in defense
60 427
60 428 genes could be associated to a mechanism of defense priming induced by the presence of
the pathogen during seed development, we selected the most differentially methylated, the
WRKY72 gene, and validated its implication in Xcf response during germination. By qRT-PCR,
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432 we analyzed the expression profile of *WRKY72* during germination in healthy seeds that germinated in presence of water versus *Xcf*. We clearly observed an over-expression of *WRKY72* at 7 days after imbibition in radicle of germinated seeds in presence of *Xcf*, showing the role of this gene in the defense response to *Xcf* infection during germination (Fig. 3D).

436 Together, these results suggested that DMRs due to the presence of Xcf were mainly located in genes that could serve during the germination process and/or to the plant immune response to Xcf. In other word, pathogen-specific DNA methylations occurring during seed development could serve as defense priming to regulate gene expressions during the germination process, including a resumption of the molecular dialogue with the pathogen.

441

Discussion

443 Seeds are essential components of plants fitness and represent an important means of pathogen dispersion. To date, seed-pathogen interactions have been understudied at the molecular level, with, to our knowledge, only one plant-orientated study describing the transcriptomic response of *Medicago truncatula* seeds to bacterial pathogens of the Xanthomonadaceae family (Terrasson et al., 2015). We thus attempted to mitigate this knowledge gap by describing the molecular dialogue between common bean seeds and Xcf in conditions that seed bacterial transmission was asymptomatic. A first central result regarding this interaction is that Xcf was able to colonize seeds without major impact on seed physiology parameters, which was reflected by similar dry weights and water contents in healthy- and infected-seeds (Fig. 1). Consequently, we could not observe any obvious morphological changes in Xcf-colonized seeds compared to mock treated samples. Such findings indicate that asymptomatic Xcf colonization does not impact seed development or alter seed growth. This is consistent with previous report in *M. truncatula* during compatible interaction with *X. euvesicatoria* pv. *alfalfae*, while incompatible interaction with *X. campestris* pv. *campestris* resulted in developmental defects alongside a strong activation of defense pathways (Terrasson et al., 2015).

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541
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560

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559
560
establish an intense molecular dialogue at the early stages of seed development that appears to become less intense as seed maturity approaches (Fig. 2).

On the pathogen side, the up-regulation of the T3SS genes and cognate effectors observed in the early stages in comparison with 42 DAP suggests they could play a role in the host defense silencing during the early step of seed colonization (Buttner, 2016). Indeed, Xanthomonads T3SS and T3Es are known to play two crucial roles in allowing efficient bacterial seed transmission (Darsonval et al., 2008) and in suppressing plant innate immunity and modulate plant pathways for the benefits of the bacteria (Büttner, 2016). Interestingly, down-regulated categories at early stages include basic biological processes such as translation, protein turnover and DNA replication. This might suggest that Xcf multiplication is hampered, consistently with the observation that number of Xcf cells in seeds does not increase significantly throughout seed developmental stages (Fig. 1C). Fewer functional categories were enriched at 35 DAP (Fig. 2D). The up-regulated ones (4 out of 5) included peptidases, glycosylases and methyl transferases. Such functions can be associated with both suppression of defense (peptidases, Figaj et al., 2019) and cell wall remodeling, which could help bacterial colonization of seed tissues, with no detectable impact on the seed physiology and morphology, although more subtle microscopical effects cannot be excluded (Fig. 1).

On the host side, we also observed intense gene expression changes at early seed developmental stage (24 DAP) in comparison to later ones, concomitantly with the intense bacterial secretion activity. We observed an enrichment of up-regulated Leucine Rich Repeat (LRR) protein kinases (2 categories out of 6, LRR class VIII and class Xb), which are known to have prominent roles in microbe perception and defense activation in non-seed tissues (Chakraborty et al., 2019), suggesting that the host may be able to recognize the pathogen. On the other hand, RNA-Seq data highlighted a down-regulation of gene categories with well characterized roles in the transduction of defense signaling pathways, including Mitogen-Activated Protein Kinases (MAPKs such as MAPKKK3, MAPK3 or MAPK4) and transcription factors of the bZIP (basic leucine ZIPper), TIFY, and AP2/ERF (APETALA 2/ Ethylene Responsive Factor) families (Bethke et al., 2009; Bai et al., 2011; Tintor et al., 2013; Noman et al., 2017). In line with this, we observed down-regulation of transcription factor families known to have wider functions in plant stress signaling, such as the TUB or TLP (TUBBY-Like Proteins) and the HSF (Heat Shock Transcription factor), as well as genes encoding PR proteins, including JAZ and JAR genes involved in the jasmonic acid pathway, and PAD4 involved in the salicylic acid pathway. Such data suggest that even the transduction components of the defense pathway are inhibited, potentially due to the bacterial T3E, ultimately avoiding a defense response.

Similar to transcriptomic data, changes in expression of small RNA at 24 DAP and 42 DAP were consistent with silencing of downstream defense gene response. Indeed, analysis
of the differentially expressed miRNA at 24 DAP and their putative target genes suggest a growth/defense trade-off mechanism in favor of growth in Xcf-inoculated seeds, with down-regulation of defense-associated transcripts (e.g. putative ortholog of PAD4 (Phvul002G274500, Phyto Alexin Deficient 4, involved in salicylic acid signaling in A. thaliana (Pruitt et al., 2021)), a pepsin-type protease (Phvul001G229200)) and up-regulation of development-associated transcripts (e.g. TOR-LIKE (Phvul011G050300) and MED15 (Phvul010G157900, MEDIATOR 15, required for correct embryogenesis in A. thaliana (Kim et al., 2016)). Interestingly, a heat shock protein (HSP70, Phvul003G154800) was detected as down-regulated genes at 24 DAP in Xcf-inoculated seeds and potential target of miR396, which complete the observed downregulation of HSF and smallHSP from our infected host transcriptome data (Fig. 2D). Recently it was showed that heat shock proteins are the most represented family among the down-regulated DEGs in leaf in a resistant common bean genotype towards common bacterial blight (caused by Xcf and Xanthomonas phaseoli pv. phaseoli) in comparison to a susceptible one (Foucher et al., 2020). On the other hand, data obtained at 42 DAP revealed only down-regulation of one miRNA family miR451, potentially regulating the up-regulation of its predicted target gene (Phvul009G100000) (Table 1). Its A. thaliana homolog (AT3G49600.1) deubiquitinates the histone H2B and is required for heterochromatin silencing during seed development (Luo et al., 2008). It is worth noting that chromatin reorganization processes due to histone modifications are among the categories enriched at 42 DAP (Fig. 2D), therefore suggesting that epigenetic regulation is a relevant component of the seed-pathogen molecular dialogue at this stage, potentially acting as priming for post-germination phase. Globally, the transcriptomic response of the susceptible host plant suggests that developing seeds are able to perceive the pathogen, and that defense responses might be largely inhibited by the bacterial T3SS arsenal. Consistent with suppression of the plant defense, up-regulation of photosynthesis and down-regulation of cell wall organization enzymes (Fig.2D) were also previously observed in leaves of susceptible common bean plants upon infection (Foucher et al., 2020). On the other hand, down-regulation of HSP and HSF, and AP2/ERF transcription factors (Fig.2D) were the hallmark of resistant plants. This suggests that a balance between susceptibility and resistance exist in Xcf-infected seeds, which could explain why, despite active bacterial colonization, the seeds were asymptomatic and presented no obvious physiological impact.

In this study, we also revealed that DNA methylation changes in Xcf-inoculated seed may also act as defense priming for post-germination phase. Indeed, the seed host methylome analysis at 42 DAP revealed significant changes in methylation status in 826 different genomic regions, affecting a total of 99 different genes, which did not display any change in gene expression during seed maturation. Of these, 17 can be associated to defense processes in
<table>
  <tr>
    <td>1</td>
    <td>540</td>
    <td>a relatively straightforward manner (Table 2). As hypomethylation of defense genes has been</td>
  </tr>
  <tr>
    <td>2</td>
    <td>541</td>
    <td>widely associated with increased resistance to biotic stress (Dowen et al., 2012; Annacondia</td>
  </tr>
  <tr>
    <td>3</td>
    <td>542</td>
    <td>et al., 2021), the hypomethylated genes of this list (10 out of 17) can be considered as</td>
  </tr>
  <tr>
    <td>4</td>
    <td>543</td>
    <td>candidates for epigenetic-dependent defense priming. The concept of defense priming</td>
  </tr>
  <tr>
    <td>5</td>
    <td>544</td>
    <td>postulates that plants conserve the memory of previous encounters with pathogens by</td>
  </tr>
  <tr>
    <td>6</td>
    <td>545</td>
    <td>preparing their defense networks to respond more rapidly and strongly to a future aggression</td>
  </tr>
  <tr>
    <td>7</td>
    <td>546</td>
    <td>(Martinez-Medina et al., 2016). Enhanced chromatin access to defense genes through</td>
  </tr>
  <tr>
    <td>8</td>
    <td>547</td>
    <td>hypomethylation is one of the best characterized mechanisms in this sense (Hannan Parker</td>
  </tr>
  <tr>
    <td>9</td>
    <td>548</td>
    <td>et al., 2022). Furthermore, epigenetic defense priming can be transmitted to the next</td>
  </tr>
  <tr>
    <td>10</td>
    <td>549</td>
    <td>generations (Slaughter et al., 2012). This would be consistent with a scenario where Xcf</td>
  </tr>
  <tr>
    <td>11</td>
    <td>550</td>
    <td>colonization does not directly induce defense gene activation in common bean seeds, but</td>
  </tr>
  <tr>
    <td>12</td>
    <td>551</td>
    <td>rather triggers a primed state that prepare defense networks for the moment when the</td>
  </tr>
  <tr>
    <td>13</td>
    <td>552</td>
    <td>pathogen will again become virulent (after germination). Hypomethylation of transposable</td>
  </tr>
  <tr>
    <td>14</td>
    <td>553</td>
    <td>elements is another well-characterized mechanism of epigenetic regulation of plant defenses,</td>
  </tr>
  <tr>
    <td>15</td>
    <td>554</td>
    <td>as it can lead to the euchromatisation of wide genomic regions, both proximal and distal (López</td>
  </tr>
  <tr>
    <td>16</td>
    <td>555</td>
    <td>Sánchez et al., 2016; Halter et al., 2021). The five defense genes present in Table 2 are thus</td>
  </tr>
  <tr>
    <td>17</td>
    <td>556</td>
    <td>likely to be good candidates for relevant roles in bean resistance against Xcf. They include</td>
  </tr>
  <tr>
    <td>18</td>
    <td>557</td>
    <td>three genes affected by hypomethylation (Phvul.008G164600, Phvul.005G163000,</td>
  </tr>
  <tr>
    <td>19</td>
    <td>558</td>
    <td>Phvul.010G026400), namely two putative LRR kinase receptors and one effector receptor, all</td>
  </tr>
  <tr>
    <td>20</td>
    <td>559</td>
    <td>uncharacterized. The other two genes affected by hypermethylation are another</td>
  </tr>
  <tr>
    <td>21</td>
    <td>560</td>
    <td>uncharacterized LRR kinase receptor (Phvul.005G162000) and the putative bean homolog of</td>
  </tr>
  <tr>
    <td>22</td>
    <td>561</td>
    <td>WRKY72 (Phvul.003G068700). This transcription factor has the highest methylation gain</td>
  </tr>
  <tr>
    <td>23</td>
    <td>562</td>
    <td>among all the genes detected (fold change of +4,3), suggesting that its methylation status</td>
  </tr>
  <tr>
    <td>24</td>
    <td>563</td>
    <td>might be important in response to Xcf infection. Indeed, the role of WRKY72 orthologs is</td>
  </tr>
  <tr>
    <td>25</td>
    <td>564</td>
    <td>contradictory in different species. A positive role on defense responses was showed in A.</td>
  </tr>
  <tr>
    <td>26</td>
    <td>565</td>
    <td>thaliana and tomato (Solanum lycopersicum) against oomycetes and bacteria, respectively</td>
  </tr>
  <tr>
    <td>27</td>
    <td>566</td>
    <td>(Bhattarai et al., 2010), but regarding the interaction between rice (Oryza sativa) and</td>
  </tr>
  <tr>
    <td>28</td>
    <td>567</td>
    <td>Xanthomonas oryzae pv. Oryzae, it was showed to negatively regulate rice defense responses</td>
  </tr>
  <tr>
    <td>29</td>
    <td>568</td>
    <td>by repressing jasmonate biosynthetic genes (Hou et al., 2019). In our study, we validated its</td>
  </tr>
  <tr>
    <td>30</td>
    <td>569</td>
    <td>role as Xcf-response genes during germination by highlighting its over-expression at 7 DAI in</td>
  </tr>
  <tr>
    <td>31</td>
    <td>570</td>
    <td>radicles of germinated seeds in presence of Xcf. Another consideration regarding our</td>
  </tr>
  <tr>
    <td>32</td>
    <td>571</td>
    <td>methylome data is the high overlap between DMRs-containing genes and germination-DEGs</td>
  </tr>
  <tr>
    <td>33</td>
    <td>572</td>
    <td>(Fig. 3B). This suggests that the DMR-containing genes following bacterial infection detected</td>
  </tr>
  <tr>
    <td>34</td>
    <td>573</td>
    <td>in this study may serve during the germination process through a defense priming mechanism.</td>
  </tr>
  <tr>
    <td>35</td>
    <td>574</td>
    <td>More investigation will be required to define if these methylation changes will have positive or</td>
  </tr>
  <tr>
    <td>36</td>
    <td>575</td>
    <td>negative impacts on defense- and/or germination-related gene expressions and will require</td>
  </tr>
  <tr>
    <td>37</td>
    <td>576</td>
    <td>extensive transcriptomic analyses.</td>
  </tr>
  <tr>
    <td>38</td>
    <td>577</td>
    <td></td>
  </tr>
  <tr>
    <td>39</td>
    <td>578</td>
    <td></td>
  </tr>
  <tr>
    <td>40</td>
    <td>579</td>
    <td></td>
  </tr>
  <tr>
    <td>41</td>
    <td>580</td>
    <td></td>
  </tr>
  <tr>
    <td>42</td>
    <td>581</td>
    <td></td>
  </tr>
  <tr>
    <td>43</td>
    <td>582</td>
    <td></td>
  </tr>
  <tr>
    <td>44</td>
    <td>583</td>
    <td></td>
  </tr>
  <tr>
    <td>45</td>
    <td>584</td>
    <td></td>
  </tr>
  <tr>
    <td>46</td>
    <td>585</td>
    <td></td>
  </tr>
  <tr>
    <td>47</td>
    <td>586</td>
    <td></td>
  </tr>
  <tr>
    <td>48</td>
    <td>587</td>
    <td></td>
  </tr>
  <tr>
    <td>49</td>
    <td>588</td>
    <td></td>
  </tr>
  <tr>
    <td>50</td>
    <td>589</td>
    <td></td>
  </tr>
  <tr>
    <td>51</td>
    <td>590</td>
    <td></td>
  </tr>
  <tr>
    <td>52</td>
    <td>591</td>
    <td></td>
  </tr>
  <tr>
    <td>53</td>
    <td>592</td>
    <td></td>
  </tr>
  <tr>
    <td>54</td>
    <td>593</td>
    <td></td>
  </tr>
  <tr>
    <td>55</td>
    <td>594</td>
    <td></td>
  </tr>
  <tr>
    <td>56</td>
    <td>595</td>
    <td></td>
  </tr>
  <tr>
    <td>57</td>
    <td>596</td>
    <td></td>
  </tr>
  <tr>
    <td>58</td>
    <td>597</td>
    <td></td>
  </tr>
  <tr>
    <td>59</td>
    <td>598</td>
    <td></td>
  </tr>
  <tr>
    <td>60</td>
    <td>599</td>
    <td></td>
  </tr>
</table>
All together, these results indicate that the molecular mechanisms involved in the pathogen-seed dialogue change radically across the developmental stages for both the host and the pathogen side, potentially suggesting the existence of distinct phases in the considered seed-pathogen interaction. It would be interesting to explore whether such pattern takes place in other seed-pathogen interactions. By summing our physiological and molecular observations, with the previous findings of Terrasson et al. (2015), we can propose a model where the recognition of a host-specific pathogen at the early stages of seed development fails to trigger seed defense activation, as if the presence of the pathogen was "accepted" by the host. Even if we cannot define whether this suppression is caused by the pathogen or by the host, two Xanthomonas studies would support the role of bacterial T3SS in host defense silencing. Darsonval et al. (2008) showed the essential role of T3E for an efficient seed colonization in the X. citri pv. fuscans-bean seed interaction and Terrasson et al. (2015) showed that X. euvesicatoria pv. Alfalfae was able to silence some defense genes in a compatible interaction, but not in an incompatible one. In any case, the result is a situation where the seed develops normally without any obvious fitness costs associated to an eventual defense activation, while the host-specific pathogen displays a non-aggressive behavior throughout all the seed development and limits its proliferation (Fig. 4). Such "ceasefire" scenario might be advantageous for both parts: the seed is able to reach maturity, which would potentially be beneficial for the pathogen as well by allowing it to infect the future germinated seedling, therefore giving it access to nourishment and facilitating its dispersal. On the other hand, data at 42 DAP suggest a relevant role for epigenetic modifications in the host. It is tempting to speculate that such modifications contribute to prepare the host to face a novel pathogen assault after germination (Fig. 4). Detailed analysis of the transcriptome and epigenome of the bean-Xcf interaction during the germination process would be a promising future research direction in this sense. Recent data from the compatible interaction *Alternaria brassicicola-A. thaliana*, used as seed transmission model, showed that host defense pathways are subjected to drastic changes during the germination process (Ortega-Cuadros et al., 2022). It would be interesting to explore whether such rearrangements take place in other compatible interactions such as Xcf-bean and if a link with epigenetic modifications exists.

To summarize, the present study adds novel elements to the current knowledge gap of seed-pathogen interactions. The dual transcriptomic analysis allowed for the first time to describe the molecular dialogue from both host and pathogen sides, while methylome and sRNAs profiling added further indications on the potential regulatory mechanisms and the genes involved. A dedicated Jbrowse containing all these generated data will serve as
baseline tool for the scientific communities and will be enriched by future related studies. An
important general conclusion that we can draw is that seeds have primarily an active role in
this interaction at early seed maturation stage, contrary to the widely diffused assumption
considering seeds as passive carriers of microbes (Dutta et al., 2014). As the role of
seedborne pathogens in causing yield losses receives relatively little attention, we hope that
the present study can stimulate novel research efforts in this sense to shed light on the many
obscure points still shrouding seed-pathogen interactions.

## Supplemental data

Supplementary Data S1: Result tables of RNA-seq data

Supplementary Data S2: Result tables of sRNA-seq data

Supplementary Data S3: Result tables of BS-seq data

Supplementary Data S4: Primers used for qPCR experiments.

## Acknowledgements

This work was supported by the French National Research Agency in the framework of the
SUCSEED project (ANR-20-PCPA-0009) and by the RFI "Objectif Végétal" supported by the
French Region Pays de la Loire, Angers Loire Métropole, and the European Regional
Development Fund. The authors wish to thank Daniel Sochard (Phenotic plateform, SFR
Quasav) for crop management, Muriel Bahut (ANAN platform, SFR Quasav) for bacterial RNA
sequencing, Sébastien Carrère (LIPME, Toulouse) for the annotation of Xcf genome
sequences and Sylvain Gaillard for the public release of the Phaseolus Jbrowse.

## Author contributions

AD, MBarret and JV designed the research. AD, MBarret and JV supervised the
experiments; AD, LPT, DL, NC, MBriand, MBarret, JV performed and analysed the
experiments. AD, LPT, NC, MBarret and JV wrote the manuscript and all co-authors
reviewed and edited the manuscript.

## Data availability

The data that support the findings of this study have been deposited in NCBI Gene Expression
Omnibus and are accessible through GEO Super Series accession number GSE227421
(https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE227421) or individually through
GEO accession numbers GSE227386 (bacterial RNA-seq,
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE227386), GSE226918 (plant
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649 RNAseq, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE226918), GSE226919

650 (plant methylome, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE226919) and

651 GSE226920 (sRNA-Seq, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE226920).

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653

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## Legends

**Figure 1. Transmission of Xcf to bean seeds.** (A) Seed water content (B) Seed dry weight (gram) and (C) Xcf population size (log₁₀ CFU per gram of seed) at the different sampling stages (24DAP, 35DAP and 42DAP). Differences between the sampling stage and the treatment (H₂O- or Xcf-inoculated) were assessed by Kruskall-Wallis test followed by post-hoc Dunn's test. The percentages of observed contaminated seeds at different seed developmental stages are indicated (expressed as averages with SD between brackets). P-values are indicated as * <5%, ** <1% and *** <0.1%.

**Figure 2. Dual transcriptomic analysis of the Xcf-P. vulgaris seed interaction.** (A) & (C) Histograms summarizing the number of differentially expressed genes (DEGs) detected comparing datasets from different seed development stages from Xcf samples (A) and DEGs from different development stages from P. vulgaris samples (C). The number of DEGs is indicated on the bars. (B) & (D) Dot plots showing category enrichment results obtained through gene ontology analysis of DEGs from Xcf (B) and P. vulgaris (D). Gene ontology analysis was performed with the Clusterprofiler package for R.

**Figure 3. Summary of methylome analysis data generated by comparing Xcf-colonized and uncolonized seeds at 42 DAP.** (A) Pie chart illustrating the repartition of differentially methylated regions (DMRs) following Xcf colonization on P. vulgaris genome at 42 DAP. (B) Venn diagram illustrating the overlap between gene sequences containing DMRs at 42 DAP and differentially expressed genes (DEGs) during germination (see details in text).
Tables

<table><caption>Table 1. Summary of differentially accumulated small RNAs (up- or down-regulated) detected at 24 and 42 DAP in *Xcf*-colonized *P. vulgaris* seeds with their putative target genes according to psRNAtarget.</caption><thead><tr><th rowspan="2">Seed developmental stages</th><th colspan="3">DEseq2</th><th rowspan="2">psRNAtarget combined with corresponding significant expression changes from RNAseq data</th></tr><tr><th>Up- or down-regulation in Xcf-colonized seeds</th><th>mature miR</th><th>variants</th></tr></thead><tbody><tr><td rowspan="10">24DAP</td><td>Up</td><td>miR162</td><td>a,b</td><td>Phvul.007G067800 (HSF), Phvul.008G055500 (TGD3), Phvul.006G176000 (trihelix DNA-binding), Phvul.008G114700 (Rab-GDF)</td></tr><tr><td>Up</td><td>miR172</td><td>a,c,d,e,f,g,h,i,l</td><td>Phvul.009G014600 (cardiolipin deacylase), Phvul.001G212400 (RING-domain E3 ligase), Phvul.005G068800 (Probable E3 ubiquitin-protein ligase), Phvul.003G053000 (glycosyltransferase)</td></tr><tr><td>Up</td><td>miR396</td><td>a,b,c,d,e,i</td><td>Phvul.009G246000 (SNF4-like), Phvul.001G229200 (pepsin-type protease), Phvul.002G026300 integrin-like protein), Phvul.003G154800 (HSP70)</td></tr><tr><td>Up</td><td>miR482</td><td>3p, b-3p, d-3p</td><td>Phvul.011G149100 Transducin/WD40 repeat-like), Phvul.008G055500 (ATPase component TGD3 of TGD), Phvul.003G295800 (ATG2-like), Phvul.011G082700 (P-loop NTPase), Phvul.010G141400 (DOF1-like TF), Phvul.002G261500 (RNA polymerase regulatory protein)</td></tr><tr><td>Up</td><td>miR6478</td><td>-</td><td>Phvul.003G155500 (component SR-alpha of SRP)</td></tr><tr><td>Up</td><td>miR8175</td><td>-</td><td>Phvul.002G059000 (Phospholipase A1), Phvul.002G274500 (PAD4-like), Phvul.010G082300 (UDP-D-glucuronic acid 4-epimerase), Phvul.005G035400 (mRNA-splicing factor 18), Phvul.001G240600 (CaLB domain)</td></tr><tr><td>Down</td><td>let7</td><td>a,c,d,f</td><td>Phvul.001G022700 (REMORIN-LIKE), Phvul.003G119100 (calcium-dependent lipid-binding), Phvul.011G061600 (PTAC16-like), Phvul.003G035400 (XYL1-like), Phvul.004G121666 (subunit of CF1 of ATP synthase), Phvul.008G163350 (cohesin cofactor (PDS5)), Phvul.011G050300 (protein kinase (PIKK) TOR-like), Phvul.003G050600 (catalytic protein (CER2)), Phvul.007G069900, Phvul.011G001200 (SAC1-like), Phvul.002G185150 (sodium:proton antiporter (SOS1))</td></tr><tr><td>Down</td><td>miR21</td><td>a</td><td>Phvul.010G157900 (MED15-like), Phvul.007G191600 (CHR8-like)</td></tr><tr><td>Down</td><td>miR2111</td><td>a,b,c,d,e,f,g,h, l,j,k,m,n,o</td><td>Phvul.001G269300 (MED13-like), Phvul.001G179300 (PGP1-like), Phvul.010G125200 (NOC1/SWA2-like), Phvul.007G168500 (Solute transport channels)</td></tr><tr><td>Down</td><td>miR482</td><td>5p</td><td>Phvul.004G170000 (STT3-like), Phvul.010G125200 (NOC1/SWA2-like), Phvul.007G244066, Phvul.002G189700 (UPL1-like)</td></tr><tr><td rowspan="3">42DAP</td><td>Up</td><td>miR31</td><td>-</td><td>-</td></tr><tr><td>Down</td><td>miR451</td><td>a</td><td>Phvul.009G100000 (UBP26-like)</td></tr><tr><td>Down</td><td>miR164</td><td>a,b,c,d,e,f,g,h, l,j,k</td><td>-</td></tr></tbody></table>

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<table><caption>Table 2. List of differentially methylated regions located in defense-associated genes in of *P. vulgaris* seeds following Xcf colonization at 42 DAP. DMRs were located in promoter or gene sequences, but also in transposable elements located within 5kb of genic regions. The *P. vulgaris* annotation column was filled according to the *P. vulgaris* genome (v2.1). The location indicates whether the region is localized in a coding region (gene) or in the promoter (1kbprom) or in TE within 5kb of genic regions (within 5kb). The putative ortholog was assigned as best hit based on sequence similarity in the *A. thaliana* genome (v.11). The “gain or loss” column shows whether the differentially methylated region associated with the corresponding *P. vulgaris* gene is hypo- (loss) or hypermethylated (gain) in response to Xcf colonization at 42 DAP. FC, fold change of methylation between Xcf- versus H2O-treated seeds. FC ratios are not indicated for DMRs within 5kb of genic regions because they correspond to multiple DMRs.</caption><thead><tr><th>P. vulgaris locus ID</th><th>Location</th><th>P. vulgaris annotation</th><th>A. thaliana putative ortholog</th><th>A. thaliana symbol</th><th>A. thaliana annotation</th><th>Methylation FC (Xcf vs H2O)</th><th>Gain or loss of methylation in Xcf-treated seeds</th></tr></thead><tbody><tr><td>Phvul.001G233000</td><td>gene</td><td>protein kinase (SD-1)</td><td>AT3G16030</td><td>CES101</td><td>lectin protein kinase family protein</td><td>0.52</td><td>loss</td></tr><tr><td>Phvul.001G215500</td><td>within 5kb</td><td>not annotated</td><td>AT5G08315</td><td></td><td>Defensin-like (DEFL) family protein</td><td></td><td>loss</td></tr><tr><td>Phvul.002G130300</td><td>within 5kb</td><td>not annotated</td><td>AT3G14470</td><td></td><td>NB-ARC domain-containing disease resistance protein</td><td></td><td>gain</td></tr><tr><td>Phvul.001G2130400</td><td>within 5kb</td><td>not annotated</td><td>AT3G14470</td><td></td><td>NB-ARC domain-containing disease resistance protein</td><td></td><td>gain</td></tr><tr><td>Phvul.003G021700</td><td>1kbProm</td><td>transferase transferring phosphorus-containing group</td><td>AT3G07040</td><td></td><td>Protein kinase superfamily protein</td><td>0.51</td><td>loss</td></tr><tr><td>Phvul.003G040300</td><td>1kbProm</td><td>6-deoxocastasterone 6-oxidase</td><td>AT3G30180</td><td>BR6OX2</td><td>brassinosteroid-6-oxidase 2</td><td>2.98</td><td>gain</td></tr><tr><td>Phvul.003G056900</td><td>within 5kb</td><td>systemic acquired resistance (SAR) regulator protein (SNI1)</td><td>AT4G18470</td><td>SNI1</td><td>negative regulator of systemic acquired resistance (SNI1)</td><td></td><td>loss</td></tr><tr><td>Phvul.003G068700</td><td>gene</td><td>transcription factor (WRKY)</td><td>AT5G15130</td><td>WRKY72</td><td>WRKY DNA-binding protein 72</td><td>4.30</td><td>gain</td></tr><tr><td>Phvul.003G068700</td><td>within 5kb</td><td>transcription factor (WRKY)</td><td>AT5G15130</td><td>WRKY72</td><td>WRKY DNA-binding protein 72</td><td></td><td>gain</td></tr><tr><td>Phvul.003G175700</td><td>gene</td><td>DRB4-DRB7.1 regulator complex component DRB7</td><td>AT5G20320</td><td>OCL4</td><td>dicer-like 4</td><td>0.37</td><td>loss</td></tr><tr><td>Phvul.004G076100</td><td>within 5kb</td><td>not annotated</td><td>AT3G14470</td><td></td><td>NB-ARC domain-containing disease resistance protein</td><td></td><td>loss</td></tr><tr><td>Phvul.004G105600</td><td>within 5kb</td><td>not annotated</td><td>AT2G34930</td><td></td><td>disease resistance family protein / LRR family protein</td><td></td><td>loss</td></tr><tr><td>Phvul.005G162000</td><td>1kbProm</td><td>transferase transferring phosphorus-containing group</td><td>AT4G29990</td><td></td><td>Leucine-rich repeat transmembrane protein kinase protein</td><td>2.36</td><td>gain</td></tr><tr><td>Phvul.005G162000</td><td>within 5kb</td><td>transferase transferring phosphorus-containing group</td><td>AT4G29990</td><td></td><td>Leucine-rich repeat transmembrane protein kinase protein</td><td></td><td>gain</td></tr><tr><td>Phvul.005G162100</td><td>within 5kb</td><td>transferase transferring phosphorus-containing group</td><td>AT3G51800</td><td></td><td>Leucine-rich repeat protein kinase family protein</td><td></td><td>gain</td></tr><tr><td>Phvul.005G163000</td><td>gene</td><td>transferase transferring phosphorus-containing group</td><td>AT4G29990</td><td></td><td>Leucine-rich repeat transmembrane protein kinase protein</td><td>0.38</td><td>loss</td></tr><tr><td>Phvul.005G163000</td><td>within 5kb</td><td>transferase transferring phosphorus-containing group</td><td>AT4G29990</td><td></td><td>Leucine-rich repeat transmembrane protein kinase protein</td><td></td><td>loss</td></tr><tr><td>Phvul.006G006800</td><td>gene</td><td>TKL protein kinase superfamily, protein kinase (DUF26)</td><td>AT4G05200</td><td>CRK25</td><td>cysteine-rich RLK (RECEPTOR-like protein kinase) 25</td><td>0.18</td><td>loss</td></tr><tr><td>Phvul.006G033200</td><td>gene</td><td>not annotated</td><td>AT5G38280</td><td>PRSK</td><td>PR5-like receptor kinase</td><td>0.52</td><td>loss</td></tr><tr><td>Phvul.007G187700</td><td>1kbProm</td><td>not annotated</td><td>AT3G04720</td><td>PR4</td><td>pathogenesis-related 4</td><td>0.37</td><td>loss</td></tr><tr><td>Phvul.007G241200</td><td>gene</td><td>transcription factor (MYB-related)</td><td>AT5G47390</td><td></td><td>myb-like transcription factor family protein</td><td>3.25</td><td>gain</td></tr><tr><td>Phvul.007G241200</td><td>within 5kb</td><td>transcription factor (MYB-related)</td><td>AT5G47390</td><td></td><td>myb-like transcription factor family protein</td><td></td><td>gain</td></tr><tr><td>Phvul.007G241300</td><td>within 5kb</td><td>Ser/Thr protein kinase</td><td>AT3G50240</td><td>FU</td><td>Protein kinase family protein with ARM repeat domain</td><td></td><td>gain</td></tr><tr><td>Phvul.008G164500</td><td>within 5kb</td><td>transferase transferring phosphorus-containing group</td><td>AT3G21340</td><td></td><td>Leucine-rich repeat protein kinase family protein</td><td></td><td>loss</td></tr><tr><td>Phvul.008G164600</td><td>gene</td><td>not annotated</td><td>AT3G05700</td><td></td><td>Leucine-rich repeat transmembrane protein kinase protein</td><td>0.45</td><td>loss</td></tr><tr><td>Phvul.008G164600</td><td>within 5kb</td><td>not annotated</td><td>AT3G05700</td><td></td><td>Leucine-rich repeat transmembrane protein kinase protein</td><td></td><td>loss</td></tr><tr><td>Phvul.008G228714</td><td>gene</td><td>S8-class protease (subtilisin) families, protease (SBT4)</td><td>AT3G46850</td><td></td><td>Subtilase family protein</td><td>4.29</td><td>gain</td></tr><tr><td>Phvul.008G229400</td><td>gene</td><td>S8-class protease (subtilisin) families, protease (SBT4)</td><td>AT5G59100</td><td></td><td>Subtilisin-like serine endopeptidase family protein</td><td>0.48</td><td>loss</td></tr><tr><td>Phvul.010G026400</td><td>1kbProm</td><td>effector receptor (NLR)</td><td>AT5G36930</td><td></td><td>Disease resistance protein (TIR-NBS-LRR class) family</td><td>0.35</td><td>loss</td></tr><tr><td>Phvul.010G026400</td><td>within 5kb</td><td>effector receptor (NLR)</td><td>AT5G36930</td><td></td><td>Disease resistance protein (TIR-NBS-LRR class) family</td><td></td><td>loss</td></tr><tr><td>Phvul.010G027900</td><td>within 5kb</td><td>effector receptor (NLR)</td><td>AT5G36930</td><td></td><td>Disease resistance protein (TIR-NBS-LRR class) family</td><td></td><td>loss</td></tr><tr><td>Phvul.010G028000</td><td>within 5kb</td><td>effector receptor (NLR)</td><td>AT5G36930</td><td></td><td>Disease resistance protein (TIR-NBS-LRR class) family</td><td></td><td>loss</td></tr><tr><td>Phvul.010G062500</td><td>within 5kb</td><td>WRKY33-dependent plant immunity transcription factor</td><td>AT2G38470</td><td>WRKY33</td><td>WRKY DNA-binding protein 33</td><td></td><td>loss</td></tr><tr><td>Phvul.011G064700</td><td>gene</td><td>U-Box E3 ligase activities, E3 ubiquitin ligase (PUB)</td><td>AT3G46510</td><td>PUB13</td><td>plant U-box 13</td><td>4.06</td><td>gain</td></tr><tr><td>Phvul.011G108300</td><td>gene</td><td>transferase transferring phosphorus-containing group</td><td>AT1G29730</td><td></td><td>Leucine-rich repeat transmembrane protein kinase</td><td>2.32</td><td>gain</td></tr><tr><td>Phvul.011G176100</td><td>gene</td><td>transferase transferring one-carbon group</td><td>AT3G11480</td><td>BSMT1</td><td>S-adenosyl-L-methionine-dependent methyltransferases family protein</td><td>2.56</td><td>gain</td></tr></tbody></table>

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<figure><img src="image_3.png" /></figure>

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<figure><img src="image_4.png" /></figure>

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<figure><img src="image_5.png" /></figure>

**Figure 1. Transmission of Xcf to bean seeds. (A)**
Seed water content (B) Seed dry weight (gram) and (C) Xcf population size (log₁₀ CFU per gram of seed) at the different sampling stages (24DAP, 35DAP and 42DAP). Differences between the sampling stage and the treatment (H₂O- or Xcf-inoculated) were assessed by Kruskall-Wallis test followed by post-hoc Dunn's test. The percentages of observed contaminated seeds at different seed developmental stages are indicated (expressed as averages with SD between brackets). P-values are indicated as * <5%, ** <1% and *** <0.1%.

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<figure><img src="image_6.png" /></figure>

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<figure><img src="image_7.png" /></figure>

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<figure><img src="image_9.png" /><figcaption>Figure 2. Dual transcriptomic analysis of the *Xcf-P. vulgaris* seed interaction.</figcaption></figure>

(A)(C) Histograms summarizing the number of differentially expressed genes (DEGs) detected comparing datasets from different seed development stages from *Xcf* samples (A) and DEGs from different development stages from *P. vulgaris* samples (C). The number of DEGs is indicated on the bars. (B) (D) Dot plots showing category enrichment results obtained through gene ontology analysis of DEGs from *Xcf* (B) and *P. vulgaris* (D). Gene ontology analysis was performed with the Clusterprofiler package for R.

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<figure><img src="image_10.png" /><figcaption>D Relative Expression of *WRKY72* during germination</figcaption></figure>

<figure><img src="image_11.png" /></figure>

**Figure 3.** Summary of methylome analysis data generated by comparing *Xcf-colonized* and *uncolonized seeds* at 42 DAP. (A) Pie chart illustrating the repartition of differentially methylated regions (DMRs) following *Xcf* colonization on *P. vulgaris* genome at 42 DAP. (B) Pie charts illustrating the number of hypo-(loss) and hyper-(gain) methylated DMRs located in annotated genes (1kb promoter or coding sequences). (C) Venn diagram illustrating the overlap between gene sequences containing DMRs at 42 DAP and differentially expressed genes (DEGs) during germination (see details in text). (D) Relative expression of *WRKY72* during germination (at 3 and 7 Days after imbibition, DAI) in H₂O- and *Xcf*-treated seeds.

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<figure><img src="image_12.png" /><figcaption>**Figure 4.** Schematic model of the Xcf-bean seed dialogue. Left panel: at early seed development stages (24 DAP), Xcf is recognized by the host. Despite the bacterial recognition, defense transduction pathways based on MAP kinases cascades (MAPKs) and transcription factors (TFs) activation are suppressed in seeds, thus failing to induce a defense reaction. Red dotted lines with flat end indicate hypothetical inhibition. Middle Panel: at 35 DAP, both the bacterial pathogen and the host plant are still transcriptionally active. Bacterial populations continue to grow, but the T3SS is no longer active, suggesting that the bacteria lowered its weapons, keeping the seed alive and healthy. Right panel: at seed maturation (42 DAP), the dialogue between Xcf and seed is much less detectable in comparison to earlier stages but epigenetic mechanisms such as DNA methylation could be active, which was observed at seed maturity by the changes in the methylation status of genes identified as involved in both defense and germination processes. This change in DNA methylation could prime genes involved in defense/germination, ultimately preparing the host for the post-germination battle with the virulent Xcf (see text for more details).</figcaption></figure>

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<table><caption>Table 2. List of differentially methylated regions located in defense-associated genes in of P. vulgaris</caption><thead><tr><th>P. vulgaris locus ID</th><th>Location</th><th>P. vulgaris annotation</th></tr></thead><tbody><tr><td>Phvul.001G233000</td><td>gene</td><td>protein kinase (SD-1)</td></tr><tr><td>Phvul.002G125500</td><td>within 5kb</td><td>not annotated</td></tr><tr><td>Phvul.002G130300</td><td>within 5kb</td><td>not annotated</td></tr><tr><td>Phvul.002G130400</td><td>within 5kb</td><td>not annotated</td></tr><tr><td>Phvul.003G021700</td><td>1kbProm</td><td>transferase transferring phosphorus-containing group</td></tr><tr><td>Phvul.003G040300</td><td>1kbProm</td><td>6-deoxocastasterone 6-oxidase</td></tr><tr><td>Phvul.003G056900</td><td>within 5kb</td><td>systemic acquired resistance (SAR) regulator protein (SNI1)</td></tr><tr><td>Phvul.003G068700</td><td>gene</td><td>transcription factor (WRKY)</td></tr><tr><td>Phvul.003G068700</td><td>within 5kb</td><td>transcription factor (WRKY)</td></tr><tr><td>Phvul.003G175700</td><td>gene</td><td>DRB4-DRB7.1 regulator complex.component DRB7</td></tr><tr><td>Phvul.004G076100</td><td>within 5kb</td><td>not annotated</td></tr><tr><td>Phvul.004G105600</td><td>within 5kb</td><td>not annotated</td></tr><tr><td>Phvul.005G162000</td><td>1kbProm</td><td>transferase transferring phosphorus-containing group</td></tr><tr><td>Phvul.005G162000</td><td>within 5kb</td><td>transferase transferring phosphorus-containing group</td></tr><tr><td>Phvul.005G162100</td><td>within 5kb</td><td>transferase transferring phosphorus-containing group</td></tr><tr><td>Phvul.005G163000</td><td>gene</td><td>transferase transferring phosphorus-containing group</td></tr><tr><td>Phvul.005G163000</td><td>within 5kb</td><td>transferase transferring phosphorus-containing group</td></tr><tr><td>Phvul.006G006800</td><td>gene</td><td>TKL protein kinase superfamily.protein kinase (DUF26)</td></tr><tr><td>Phvul.006G033200</td><td>gene</td><td>not annotated</td></tr><tr><td>Phvul.007G187700</td><td>1kbProm</td><td>not annotated</td></tr><tr><td>Phvul.007G241200</td><td>gene</td><td>transcription factor (MYB-related)</td></tr><tr><td>Phvul.007G241200</td><td>within 5kb</td><td>transcription factor (MYB-related)</td></tr><tr><td>Phvul.007G241300</td><td>within 5kb</td><td>Ser/Thr protein kinase</td></tr><tr><td>Phvul.008G164500</td><td>within 5kb</td><td>transferase transferring phosphorus-containing group</td></tr><tr><td>Phvul.008G164600</td><td>gene</td><td>not annotated</td></tr><tr><td>Phvul.008G164600</td><td>within 5kb</td><td>not annotated</td></tr><tr><td>Phvul.008G228714</td><td>gene</td><td>S8-class protease (subtilisin) families.protease (SBT4)</td></tr><tr><td>Phvul.008G229400</td><td>gene</td><td>S8-class protease (subtilisin) families.protease (SBT4)</td></tr><tr><td>Phvul.010G026400</td><td>1kbProm</td><td>effector receptor (NLR)</td></tr><tr><td>Phvul.010G026400</td><td>within 5kb</td><td>effector receptor (NLR)</td></tr><tr><td>Phvul.010G027900</td><td>within 5kb</td><td>effector receptor (NLR)</td></tr><tr><td>Phvul.010G028000</td><td>within 5kb</td><td>effector receptor (NLR)</td></tr><tr><td>Phvul.010G062500</td><td>within 5kb</td><td>WRKY33-dependent plant immunity transcription factor</td></tr><tr><td>Phvul.011G064700</td><td>gene</td><td>U-Box E3 ligase activities.E3 ubiquitin ligase (PUB)</td></tr><tr><td>Phvul.011G108300</td><td>gene</td><td>transferase transferring phosphorus-containing group</td></tr><tr><td>Phvul.011G176100</td><td>gene</td><td>transferase transferring one-carbon group</td></tr></tbody></table>
garis seeds following Xcf colonization at 42 DAP. DMRs were located in promoter or gene sequence:

<table><thead><tr><th>A. thaliana putative ortholog</th><th>A. thaliana symbol</th><th>A. thaliana annotation</th></tr></thead><tbody><tr><td>AT3G16030</td><td>CES101</td><td>lectin protein kinase family protein</td></tr><tr><td>AT5G08315</td><td></td><td>Defensin-like (DEFL) family protein</td></tr><tr><td>AT3G14470</td><td></td><td>NB-ARC domain-containing disease resistance protein</td></tr><tr><td>AT3G14470</td><td></td><td>NB-ARC domain-containing disease resistance protein</td></tr><tr><td>AT1G70740</td><td></td><td>Protein kinase superfamily protein</td></tr><tr><td>AT3G30180</td><td>BR6OX2</td><td>brassinosteroid-6-oxidase 2</td></tr><tr><td>AT4G18470</td><td>SNI1</td><td>negative regulator of systemic acquired resistance (SNI1)</td></tr><tr><td>AT5G15130</td><td>WRKY72</td><td>WRKY DNA-binding protein 72</td></tr><tr><td>AT5G15130</td><td>WRKY72</td><td>WRKY DNA-binding protein 72</td></tr><tr><td>AT5G20320</td><td>DCL4</td><td>dicer-like 4</td></tr><tr><td>AT3G14470</td><td></td><td>NB-ARC domain-containing disease resistance protein</td></tr><tr><td>AT2G34930</td><td></td><td>disease resistance family protein / LRR family protein</td></tr><tr><td>AT4G29990</td><td></td><td>Leucine-rich repeat transmembrane protein kinase protein</td></tr><tr><td>AT4G29990</td><td></td><td>Leucine-rich repeat transmembrane protein kinase protein</td></tr><tr><td>AT1G51800</td><td></td><td>Leucine-rich repeat protein kinase family protein</td></tr><tr><td>AT4G29990</td><td></td><td>Leucine-rich repeat transmembrane protein kinase protein</td></tr><tr><td>AT4G29990</td><td></td><td>Leucine-rich repeat transmembrane protein kinase protein</td></tr><tr><td>AT4G05200</td><td>CRK25</td><td>cysteine-rich RLK (RECEPTOR-like protein kinase) 25</td></tr><tr><td>AT5G38280</td><td>PR5K</td><td>PR5-like receptor kinase</td></tr><tr><td>AT3G04720</td><td>PR4</td><td>pathogenesis-related 4</td></tr><tr><td>AT5G47390</td><td></td><td>myb-like transcription factor family protein</td></tr><tr><td>AT5G47390</td><td></td><td>myb-like transcription factor family protein</td></tr><tr><td>AT1G50240</td><td>FU</td><td>Protein kinase family protein with ARM repeat domain</td></tr><tr><td>AT3G21340</td><td></td><td>Leucine-rich repeat protein kinase family protein</td></tr><tr><td>AT1G05700</td><td></td><td>Leucine-rich repeat transmembrane protein kinase protein</td></tr><tr><td>AT1G05700</td><td></td><td>Leucine-rich repeat transmembrane protein kinase protein</td></tr><tr><td>AT3G46850</td><td></td><td>Subtilase family protein</td></tr><tr><td>AT5G59100</td><td></td><td>Subtilisin-like serine endopeptidase family protein</td></tr><tr><td>AT5G36930</td><td></td><td>Disease resistance protein (TIR-NBS-LRR class) family</td></tr><tr><td>AT5G36930</td><td></td><td>Disease resistance protein (TIR-NBS-LRR class) family</td></tr><tr><td>AT5G36930</td><td></td><td>Disease resistance protein (TIR-NBS-LRR class) family</td></tr><tr><td>AT5G36930</td><td></td><td>Disease resistance protein (TIR-NBS-LRR class) family</td></tr><tr><td>AT2G38470</td><td>WRKY33</td><td>WRKY DNA-binding protein 33</td></tr><tr><td>AT3G46510</td><td>PUB13</td><td>plant U-box 13</td></tr><tr><td>AT1G29730</td><td></td><td>Leucine-rich repeat transmembrane protein kinase</td></tr><tr><td>AT3G11480</td><td>BSMT1</td><td>S-adenosyl-L-methionine-dependent methyltransferases family protein</td></tr></tbody></table>
s, but also in transposable elements located within 5kb of genic regions. The P. vulgaris annotation column

<table><thead><tr><th>Methylatio<br>n FC (Xcf vs<br>H2O)</th><th>Gain or loss of<br>methylation in<br>Xcf-treated seeds</th></tr></thead><tbody><tr><td rowspan="4">0.52</td><td>loss</td></tr><tr><td>loss</td></tr><tr><td>gain</td></tr><tr><td>gain</td></tr><tr><td rowspan="2">0.51</td><td>loss</td></tr><tr><td>gain</td></tr><tr><td rowspan="2">2.98</td><td>loss</td></tr><tr><td>gain</td></tr><tr><td rowspan="2">4.30</td><td>loss</td></tr><tr><td>gain</td></tr><tr><td rowspan="2">0.37</td><td>loss</td></tr><tr><td>loss</td></tr><tr><td rowspan="2">2.36</td><td>loss</td></tr><tr><td>gain</td></tr><tr><td rowspan="2">0.38</td><td>loss</td></tr><tr><td>loss</td></tr><tr><td rowspan="2">0.18</td><td>loss</td></tr><tr><td>loss</td></tr><tr><td rowspan="2">0.37</td><td>loss</td></tr><tr><td>gain</td></tr><tr><td rowspan="2">3.25</td><td>loss</td></tr><tr><td>gain</td></tr><tr><td rowspan="2">0.45</td><td>loss</td></tr><tr><td>loss</td></tr><tr><td rowspan="2">4.29</td><td>gain</td></tr><tr><td>loss</td></tr><tr><td rowspan="2">0.48</td><td>loss</td></tr><tr><td>loss</td></tr><tr><td rowspan="2">0.35</td><td>loss</td></tr><tr><td>loss</td></tr><tr><td rowspan="2">4.06</td><td>gain</td></tr><tr><td>gain</td></tr><tr><td rowspan="2">2.32</td><td>gain</td></tr><tr><td>gain</td></tr><tr><td rowspan="2">2.56</td><td>gain</td></tr><tr><td>gain</td></tr></tbody></table>

<table><thead><tr><th></th><th>loss</th><th>gain</th></tr></thead><tbody><tr><td>Protein kinases</td><td>6</td><td>2</td></tr><tr><td>Resistance proteins</td><td>1</td><td>0</td></tr><tr><td>Transcription factors</td><td>0</td><td>1</td></tr><tr><td>Antimicrobial proteins</td><td>2</td><td>1</td></tr><tr><td></td><td>loss</td><td>gain</td></tr><tr><td>Protein kinases</td><td>4</td><td>3</td></tr><tr><td>Resistance proteins</td><td>4</td><td>2</td></tr><tr><td>Transcription factors</td><td>1</td><td>1</td></tr><tr><td>Antimicrobial proteins</td><td>1</td><td>0</td></tr></tbody></table>
In was filled according to the P. vulgaris genome (v2.1). The location indicates whether the region is located in the genome.

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Do not distribute
ocalized in a coding region (gene) or in the promoter (1kbprom) or in TE within 5kb of genic regions (

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Do not distribute
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Do not distribute
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Do not distribute
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Do not distribute
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Multiple DMR

Do not distribute
<table><thead><tr><th rowspan="2">Seed developmental stages</th><th colspan="3">DEseq2</th></tr><tr><th>Up- or down-regulation in Xcf-colonized seeds</th><th>mature miR</th><th>variants</th></tr></thead><tbody><tr><td rowspan="7">24DAP</td><td>Up</td><td>miR162</td><td>a,b</td></tr><tr><td>Up</td><td>miR172</td><td>a,c,d,e,f,g,h,i,l</td></tr><tr><td>Up</td><td>miR396</td><td>a,b,c,d,e,i</td></tr><tr><td>Up</td><td>miR482</td><td>3p, b-3p, d-3p</td></tr><tr><td>Up</td><td>miR6478</td><td>-</td></tr><tr><td>Up</td><td>miR8175</td><td>-</td></tr><tr><td>Down</td><td>let7</td><td>a,c,d,f</td></tr><tr><td rowspan="4">42DAP</td><td>Down</td><td>miR21</td><td>a</td></tr><tr><td>Down</td><td>miR2111</td><td>a,b,c,d,e,f,g,h,i,j,k,m,n,o</td></tr><tr><td>Down</td><td>miR482</td><td>5p</td></tr><tr><td>Up</td><td>miR31</td><td>-</td></tr><tr><td>Down</td><td>miR451</td><td>a</td></tr><tr><td>Down</td><td>miR164</td><td>a,b,c,d,e,f,g,h,i,j,k</td></tr></tbody></table>

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psRNAtarget combined with corresponding significant expression changes from RNAseq data

putative targets using psRNAtarget (= miRNA potential target genes)

Phvul.007G067800 (HSP), Phvul.008G055500 (TGD3), Phvul.006G176000 (trifunction DNA-binding), Phvul.008G114700 (Rab GDE)

Phvul.009G014600 (cardiolipin deacylase), Phvul.001G212400 (RING-domain E3 ligase), Phvul.005G068800 (Probable E3 ubiquitin-protein ligase), Phvul.003G053000 (glycosyltransferase)

Phvul.009G246000 (SNF4-like), Phvul.001G229200 (pepsin-type protease), Phvul.002G026300 integrin-like protein), Phvul.003G154800 (HSP70)

Phvul.011G149100 Transducin/WD40 repeat-like), Phvul.008G055500 (ATPase component TGD3 of TGD), Phvul.003G295800 (ATG2-like), Phvul.011G082700 (P-loop NTPase), Phvul.010G141400 (DOF1-like TF), Phvul.002G261500 (RNA polymerase regulatory protein)

Phvul.003G155500 (component SR-alpha of SRP)

Phvul.002G059000 (Phosphoinositide 1), Phvul.002G274500 (PAD4-like), Phvul.010G082300 (ODP-D-glucuronic acid 4-epimerase), Phvul.005G035400 (mRNA-splicing factor 18), Phvul.001G240600 (CalB domain)

Phvul.001G022700 (REMORIN-LIKE), Phvul.003G119100 (calcium-dependent lipid-binding), Phvul.011G061600 (PTAC16-like), Phvul.003G035400 (XYL1-like), Phvul.004G121666 (subunit of CF1 of ATP synthase), Phvul.008G163350 (cohesin cofactor (PDS5)), Phvul.011G050300 (protein kinase (PIKK) TOR-like), Phvul.003G050600 (catalytic protein (CER2)), Phvul.007G069900, Phvul.011G001200 (SAC1-like), Phvul.002G185150 (sodium-proton antiporter (SOS1))

Phvul.010G157900 (MED15-like), Phvul.007G191600 (CHR8-like)

Phvul.001G269300 (MED13-like), Phvul.001G179300 (PGP1-like), Phvul.010G125200 (NOC1/SWA2-like), Phvul.007G168500 (Solute transport channels)

Phvul.004G170000 (STT3-like), Phvul.010G125200 (NOC1/SWA2-like), Phvul.007G244066, Phvul.002G189700 (UPI1-like)

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Phvul.009G100000 (UBP26-like)

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59
60
<table><thead><tr><th>Id</th><th>H24_1</th><th>H24_2</th><th>H24_3</th><th>X24_1</th><th>X24_2</th></tr></thead><tbody><tr><td>csi-miR482e-3p</td><td>492</td><td>0</td><td>0</td><td>1</td><td>0</td></tr><tr><td>ata-miR5168-3p</td><td>314</td><td>1</td><td>0</td><td>2</td><td>1</td></tr><tr><td>bdi-miR166h-3p</td><td>314</td><td>1</td><td>0</td><td>2</td><td>1</td></tr><tr><td>osa-miR166k-3p</td><td>314</td><td>1</td><td>0</td><td>2</td><td>1</td></tr><tr><td>osa-miR166l-3p</td><td>314</td><td>1</td><td>0</td><td>2</td><td>1</td></tr><tr><td>sbi-miR166e</td><td>314</td><td>1</td><td>0</td><td>2</td><td>1</td></tr><tr><td>sbi-miR166g</td><td>314</td><td>1</td><td>0</td><td>2</td><td>1</td></tr><tr><td>zma-miR166j-3p</td><td>314</td><td>1</td><td>0</td><td>2</td><td>1</td></tr><tr><td>zma-miR166k-3p</td><td>314</td><td>1</td><td>0</td><td>2</td><td>1</td></tr><tr><td>zma-miR166n-3p</td><td>314</td><td>1</td><td>0</td><td>2</td><td>1</td></tr><tr><td>ami-let-7f-5p</td><td>53</td><td>36</td><td>153</td><td>10</td><td>39</td></tr><tr><td>ggo-let-7f</td><td>53</td><td>36</td><td>153</td><td>10</td><td>39</td></tr><tr><td>oar-let-7f</td><td>53</td><td>36</td><td>153</td><td>10</td><td>39</td></tr><tr><td>efu-let-7c</td><td>106</td><td>111</td><td>486</td><td>62</td><td>89</td></tr><tr><td>abu-let-7c</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>aca-let-7c-5p</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>ami-let-7c-5p</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>bta-let-7c</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>cfa-let-7c</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>chi-let-7c-5p</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>cja-let-7c</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>cli-let-7c-5p</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>cpi-let-7c-5p</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>cpo-let-7c-5p</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>dma-let-7c</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>dno-let-7c-5p</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>dre-let-7c-5p</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>eca-let-7c</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>gga-let-7c-5p</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>gmo-let-7c-5p</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>hhi-let-7c</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>hsa-let-7c-5p</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>ipu-let-7c</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>mml-let-7c-5p</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>mmr-let-7c</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>mmu-let-7c-5p</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>mze-let-7c</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>nbr-let-7c</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>nle-let-7c</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>oan-let-7c-5p</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>oar-let-7c</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr><tr><td>ocu-let-7c-5p</td><td>96</td><td>107</td><td>463</td><td>60</td><td>82</td></tr></tbody></table>
<table>
  <tr>
    <td>1</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>2</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>3</td>
    <td>oga-let-7c</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>4</td>
    <td>oha-let-7c-5p</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>5</td>
    <td>oni-let-7c</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>6</td>
    <td>pal-let-7c-5p</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>7</td>
    <td>pbv-let-7c-5p</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>8</td>
    <td>pha-let-7c</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>9</td>
    <td>pny-let-7c</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>10</td>
    <td>pol-let-7a-5p</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>11</td>
    <td>ppa-let-7c</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>12</td>
    <td>ppy-let-7c</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>13</td>
    <td>ptr-let-7c</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>14</td>
    <td>rno-let-7c-5p</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>15</td>
    <td>ssa-let-7c-5p</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>16</td>
    <td>ssc-let-7c</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>17</td>
    <td>tcf-let-7-5p</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>18</td>
    <td>tgu-let-7c-5p</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>19</td>
    <td>xla-let-7c-5p</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>20</td>
    <td>xtr-let-7c</td>
    <td>96</td>
    <td>107</td>
    <td>463</td>
    <td>60</td>
    <td>82</td>
  </tr>
  <tr>
    <td>21</td>
    <td>efu-let-7f</td>
    <td>174</td>
    <td>117</td>
    <td>380</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>22</td>
    <td>aca-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>23</td>
    <td>bta-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>24</td>
    <td>cfa-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>25</td>
    <td>cgr-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>26</td>
    <td>chi-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>27</td>
    <td>cja-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>28</td>
    <td>cli-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>29</td>
    <td>cpi-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>30</td>
    <td>cpo-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>31</td>
    <td>dma-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>32</td>
    <td>dno-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>33</td>
    <td>dre-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>34</td>
    <td>eca-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>35</td>
    <td>gga-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>36</td>
    <td>gmo-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>37</td>
    <td>hsa-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>38</td>
    <td>ipu-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>39</td>
    <td>mdo-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>40</td>
    <td>mml-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>41</td>
    <td>mmr-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>42</td>
    <td>mmu-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>43</td>
    <td>nle-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>44</td>
    <td>ocu-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>45</td>
    <td>ipu-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>46</td>
    <td>moa-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>47</td>
    <td>mmu-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>48</td>
    <td>mmr-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>49</td>
    <td>mmu-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>50</td>
    <td>nle-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>51</td>
    <td>ocu-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>52</td>
    <td>ocu-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>53</td>
    <td>ocu-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>54</td>
    <td>ocu-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>55</td>
    <td>ocu-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>56</td>
    <td>ocu-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>57</td>
    <td>ocu-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>58</td>
    <td>ocu-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>59</td>
    <td>ocu-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>60</td>
    <td>ocu-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
</table>
<table>
  <tr>
    <td>1</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>2</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>3</td>
    <td>oga-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>4</td>
    <td>oha-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>5</td>
    <td>oni-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>6</td>
    <td>pal-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>7</td>
    <td>pbv-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>8</td>
    <td>pha-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>9</td>
    <td>ppa-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>10</td>
    <td>ppy-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>11</td>
    <td>ptr-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>12</td>
    <td>rno-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>13</td>
    <td>sbo-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>14</td>
    <td>ssc-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>15</td>
    <td>tch-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>16</td>
    <td>tgu-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>17</td>
    <td>xla-let-7f-5p</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>18</td>
    <td>xtr-let-7f</td>
    <td>172</td>
    <td>115</td>
    <td>379</td>
    <td>52</td>
    <td>105</td>
  </tr>
  <tr>
    <td>19</td>
    <td>aly-miR2111a-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>20</td>
    <td>aly-miR2111b-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>21</td>
    <td>aly-miR2111c-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>22</td>
    <td>ath-miR2111a-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>23</td>
    <td>ath-miR2111b-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>24</td>
    <td>bna-miR2111a-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>25</td>
    <td>bna-miR2111b-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>26</td>
    <td>bna-miR2111d</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>27</td>
    <td>bra-miR2111a-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>28</td>
    <td>bra-miR2111b-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>29</td>
    <td>cme-miR2111a</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>30</td>
    <td>cme-miR2111b</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>31</td>
    <td>gma-miR2111b</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>32</td>
    <td>gma-miR2111c</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>33</td>
    <td>gma-miR2111e</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>34</td>
    <td>gma-miR2111f</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>35</td>
    <td>lja-miR2111-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>36</td>
    <td>mdm-miR2111a</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>37</td>
    <td>mdm-miR2111b</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>38</td>
    <td>mes-miR2111a</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>39</td>
    <td>mes-miR2111b</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>40</td>
    <td>mes-miR2111c</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>41</td>
    <td>mes-miR2111e</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>42</td>
    <td>mes-miR2111f</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>43</td>
    <td>mtr-miR2111a-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>44</td>
    <td>mtr-miR2111b-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>45</td>
    <td>mtr-miR2111d</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>46</td>
    <td>mdm-miR2111a</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>47</td>
    <td>mdm-miR2111b</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>48</td>
    <td>mes-miR2111a</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>49</td>
    <td>mes-miR2111b</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>50</td>
    <td>mtr-miR2111a</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>51</td>
    <td>mtr-miR2111b</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>52</td>
    <td>mtr-miR2111a-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>53</td>
    <td>mtr-miR2111b</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>54</td>
    <td>mtr-miR2111c</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>55</td>
    <td>mtr-miR2111d-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>56</td>
    <td>mtr-miR2111e-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>57</td>
    <td>mtr-miR2111f</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>58</td>
    <td>mtr-miR2111a-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>59</td>
    <td>mtr-miR2111b</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>60</td>
    <td>mtr-miR2111c</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
</table>
<table>
  <tr>
    <td>1</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>2</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>3</td>
    <td>mtr-miR2111g-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>4</td>
    <td>mtr-miR2111h</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>5</td>
    <td>mtr-miR2111i</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>6</td>
    <td>mtr-miR2111j</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>7</td>
    <td>mtr-miR2111k</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>8</td>
    <td>mtr-miR2111m-5p</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>9</td>
    <td>mtr-miR2111n</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>10</td>
    <td>mtr-miR2111o</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>11</td>
    <td>ppe-miR2111a</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>12</td>
    <td>ppe-miR2111b</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>13</td>
    <td>ppe-miR2111c</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>14</td>
    <td>ppe-miR2111d</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>15</td>
    <td>tcc-miR2111</td>
    <td>555</td>
    <td>707</td>
    <td>456</td>
    <td>105</td>
    <td>264</td>
  </tr>
  <tr>
    <td>16</td>
    <td>csi-miR2111-5p</td>
    <td>509</td>
    <td>626</td>
    <td>412</td>
    <td>92</td>
    <td>239</td>
  </tr>
  <tr>
    <td>17</td>
    <td>ptc-miR2111a</td>
    <td>509</td>
    <td>626</td>
    <td>412</td>
    <td>92</td>
    <td>239</td>
  </tr>
  <tr>
    <td>18</td>
    <td>ptc-miR2111b</td>
    <td>509</td>
    <td>626</td>
    <td>412</td>
    <td>92</td>
    <td>239</td>
  </tr>
  <tr>
    <td>19</td>
    <td>fve-miR2111a</td>
    <td>509</td>
    <td>626</td>
    <td>411</td>
    <td>92</td>
    <td>239</td>
  </tr>
  <tr>
    <td>20</td>
    <td>fve-miR2111b-5p</td>
    <td>509</td>
    <td>626</td>
    <td>411</td>
    <td>92</td>
    <td>239</td>
  </tr>
  <tr>
    <td>21</td>
    <td>fve-miR2111c</td>
    <td>509</td>
    <td>626</td>
    <td>411</td>
    <td>92</td>
    <td>239</td>
  </tr>
  <tr>
    <td>22</td>
    <td>efu-let-7d</td>
    <td>73</td>
    <td>45</td>
    <td>170</td>
    <td>27</td>
    <td>42</td>
  </tr>
  <tr>
    <td>23</td>
    <td>bta-let-7d</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>24</td>
    <td>cgr-let-7d-5p</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>25</td>
    <td>chi-let-7d-5p</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>26</td>
    <td>cja-let-7d</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>27</td>
    <td>cli-let-7d-5p</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>28</td>
    <td>cpi-let-7d-5p</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>29</td>
    <td>cpo-let-7d-5p</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>30</td>
    <td>dno-let-7d-5p</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>31</td>
    <td>eca-let-7d</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>32</td>
    <td>hsa-let-7d-5p</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>33</td>
    <td>mml-let-7d</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>34</td>
    <td>mmu-let-7d-5p</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>35</td>
    <td>oan-let-7d-5p</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>36</td>
    <td>ocu-let-7d-5p</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>37</td>
    <td>oha-let-7d-5p</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>38</td>
    <td>pbv-let-7d-5p</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>39</td>
    <td>ppy-let-7d</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>40</td>
    <td>ptr-let-7d</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>41</td>
    <td>rno-let-7d-5p</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>42</td>
    <td>ssc-let-7d-5p</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>43</td>
    <td>tgu-let-7d-5p</td>
    <td>65</td>
    <td>44</td>
    <td>166</td>
    <td>25</td>
    <td>42</td>
  </tr>
  <tr>
    <td>44</td>
    <td>pvu-miR482-5p</td>
    <td>395</td>
    <td>278</td>
    <td>197</td>
    <td>141</td>
    <td>106</td>
  </tr>
  <tr>
    <td>45</td>
    <td>bta-miR-21-5p</td>
    <td>5527</td>
    <td>1944</td>
    <td>4924</td>
    <td>1486</td>
    <td>1872</td>
  </tr>
</table>
<table>
  <tr>
    <td>1</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>2</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>3</td>
    <td>cpo-miR-21-5p</td>
    <td>5527</td>
    <td>1944</td>
    <td>4924</td>
    <td>1486</td>
    <td>1872</td>
  </tr>
  <tr>
    <td>4</td>
    <td>dno-miR-21-5p</td>
    <td>5527</td>
    <td>1944</td>
    <td>4924</td>
    <td>1486</td>
    <td>1872</td>
  </tr>
  <tr>
    <td>5</td>
    <td>ocu-miR-21-5p</td>
    <td>5527</td>
    <td>1944</td>
    <td>4924</td>
    <td>1486</td>
    <td>1872</td>
  </tr>
  <tr>
    <td>6</td>
    <td>sha-miR-21</td>
    <td>5527</td>
    <td>1944</td>
    <td>4924</td>
    <td>1486</td>
    <td>1872</td>
  </tr>
  <tr>
    <td>7</td>
    <td>ami-miR-21-5p</td>
    <td>5489</td>
    <td>1934</td>
    <td>4893</td>
    <td>1478</td>
    <td>1859</td>
  </tr>
  <tr>
    <td>8</td>
    <td>chi-miR-21-5p</td>
    <td>5489</td>
    <td>1934</td>
    <td>4893</td>
    <td>1478</td>
    <td>1859</td>
  </tr>
  <tr>
    <td>9</td>
    <td>dom-miR-21</td>
    <td>5489</td>
    <td>1934</td>
    <td>4893</td>
    <td>1478</td>
    <td>1859</td>
  </tr>
  <tr>
    <td>10</td>
    <td>mmr-miR-21</td>
    <td>5489</td>
    <td>1934</td>
    <td>4893</td>
    <td>1478</td>
    <td>1859</td>
  </tr>
  <tr>
    <td>11</td>
    <td>nle-miR-21</td>
    <td>5489</td>
    <td>1934</td>
    <td>4893</td>
    <td>1478</td>
    <td>1859</td>
  </tr>
  <tr>
    <td>12</td>
    <td>oar-miR-21</td>
    <td>5489</td>
    <td>1934</td>
    <td>4893</td>
    <td>1478</td>
    <td>1859</td>
  </tr>
  <tr>
    <td>13</td>
    <td>oga-miR-21</td>
    <td>5489</td>
    <td>1934</td>
    <td>4893</td>
    <td>1478</td>
    <td>1859</td>
  </tr>
  <tr>
    <td>14</td>
    <td>pbv-miR-21-5p</td>
    <td>5489</td>
    <td>1934</td>
    <td>4893</td>
    <td>1478</td>
    <td>1859</td>
  </tr>
  <tr>
    <td>15</td>
    <td>pha-miR-21</td>
    <td>5489</td>
    <td>1934</td>
    <td>4893</td>
    <td>1478</td>
    <td>1859</td>
  </tr>
  <tr>
    <td>16</td>
    <td>sbo-miR-21</td>
    <td>5489</td>
    <td>1934</td>
    <td>4893</td>
    <td>1478</td>
    <td>1859</td>
  </tr>
  <tr>
    <td>17</td>
    <td>aca-miR-21-5p</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>18</td>
    <td>age-miR-21</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>19</td>
    <td>cfa-miR-21</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>20</td>
    <td>cgr-miR-21-5p</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>21</td>
    <td>cja-miR-21</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>22</td>
    <td>cli-miR-21-5p</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>23</td>
    <td>cpi-miR-21-5p</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>24</td>
    <td>eca-miR-21</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>25</td>
    <td>gga-miR-21-5p</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>26</td>
    <td>ggo-miR-21</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>27</td>
    <td>hsa-miR-21-5p</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>28</td>
    <td>mdo-miR-21-5p</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>29</td>
    <td>mml-miR-21-5p</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>30</td>
    <td>mmu-miR-21a-5p</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>31</td>
    <td>mne-miR-21</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>32</td>
    <td>oan-miR-21-5p</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>33</td>
    <td>oha-miR-21-5p</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>34</td>
    <td>pal-miR-21-5p</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>35</td>
    <td>ppa-miR-21</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>36</td>
    <td>ppy-miR-21</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>37</td>
    <td>ptr-miR-21</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>38</td>
    <td>rno-miR-21-5p</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>39</td>
    <td>ssc-miR-21-5p</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>40</td>
    <td>tch-miR-21-5p</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>41</td>
    <td>tgu-miR-21-5p</td>
    <td>1292</td>
    <td>407</td>
    <td>432</td>
    <td>239</td>
    <td>354</td>
  </tr>
  <tr>
    <td>42</td>
    <td>efu-miR-34a</td>
    <td>175</td>
    <td>95</td>
    <td>221</td>
    <td>65</td>
    <td>86</td>
  </tr>
  <tr>
    <td>43</td>
    <td>ami-miR-34a-5p</td>
    <td>174</td>
    <td>95</td>
    <td>220</td>
    <td>65</td>
    <td>86</td>
  </tr>
  <tr>
    <td>44</td>
    <td>cja-miR-34a</td>
    <td>174</td>
    <td>95</td>
    <td>220</td>
    <td>65</td>
    <td>86</td>
  </tr>
  <tr>
    <td>45</td>
    <td>gga-miR-34a-5p</td>
    <td>174</td>
    <td>95</td>
    <td>220</td>
    <td>65</td>
    <td>86</td>
  </tr>
</table>
<table>
  <tr>
    <td>1</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>2</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>3</td>
    <td>mdo-miR-34a-5p</td>
    <td>174</td>
    <td>95</td>
    <td>220</td>
    <td>65</td>
    <td>86</td>
  </tr>
  <tr>
    <td>4</td>
    <td>oha-miR-34a-5p</td>
    <td>174</td>
    <td>95</td>
    <td>220</td>
    <td>65</td>
    <td>86</td>
  </tr>
  <tr>
    <td>5</td>
    <td>tch-miR-34a-5p</td>
    <td>174</td>
    <td>95</td>
    <td>220</td>
    <td>65</td>
    <td>86</td>
  </tr>
  <tr>
    <td>6</td>
    <td>xtr-miR-34a</td>
    <td>174</td>
    <td>95</td>
    <td>220</td>
    <td>65</td>
    <td>86</td>
  </tr>
  <tr>
    <td>7</td>
    <td>ame-let-7-5p</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>8</td>
    <td>bdo-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>9</td>
    <td>bmo-let-7-5p</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>10</td>
    <td>cte-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>11</td>
    <td>dan-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>12</td>
    <td>der-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>13</td>
    <td>dgr-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>14</td>
    <td>dme-let-7-5p</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>15</td>
    <td>dmo-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>16</td>
    <td>dpe-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>17</td>
    <td>dps-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>18</td>
    <td>dqu-let-7-5p</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>19</td>
    <td>dse-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>20</td>
    <td>dsi-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>21</td>
    <td>dvi-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>22</td>
    <td>dwi-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>23</td>
    <td>dya-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>24</td>
    <td>ggo-let-7a</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>25</td>
    <td>isc-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>26</td>
    <td>angi-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>27</td>
    <td>nvi-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>28</td>
    <td>ola-let-7a-5p</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>29</td>
    <td>sha-let-7a</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>63</td>
  </tr>
  <tr>
    <td>30</td>
    <td>hme-let-7</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>64</td>
  </tr>
  <tr>
    <td>31</td>
    <td>pca-let-7-5p</td>
    <td>80</td>
    <td>75</td>
    <td>204</td>
    <td>53</td>
    <td>64</td>
  </tr>
  <tr>
    <td>32</td>
    <td>abu-miR-34</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>33</td>
    <td>age-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>34</td>
    <td>bta-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>35</td>
    <td>ccr-miR-34</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>36</td>
    <td>cfa-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>37</td>
    <td>cgr-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>38</td>
    <td>chi-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>39</td>
    <td>cli-miR-34a-5p</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>40</td>
    <td>cpi-miR-34a-5p</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>41</td>
    <td>cpo-miR-34a-5p</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>42</td>
    <td>dno-miR-34a-5p</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>43</td>
    <td>dre-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>44</td>
    <td>eca-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>45</td>
    <td>ggo-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
</table>
<table>
  <tr>
    <td>1</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>2</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>3</td>
    <td>gmo-miR-34-5p</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>4</td>
    <td>hsa-miR-34a-5p</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>5</td>
    <td>ipu-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>6</td>
    <td>lla-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>7</td>
    <td>mml-miR-34a-5p</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>8</td>
    <td>mmr-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>9</td>
    <td>mmr-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>10</td>
    <td>mmu-miR-34a-5p</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>11</td>
    <td>mne-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>12</td>
    <td>mze-miR-34</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>13</td>
    <td>nbr-miR-34</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>14</td>
    <td>nle-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>15</td>
    <td>ocu-miR-34a-5p</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>16</td>
    <td>oga-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>17</td>
    <td>oni-miR-34</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>18</td>
    <td>pal-miR-34a-5p</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>19</td>
    <td>pbv-miR-34a-5p</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>20</td>
    <td>pyu-miR-34</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>21</td>
    <td>ppa-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>22</td>
    <td>ppy-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>23</td>
    <td>ptr-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>24</td>
    <td>rno-miR-34a-5p</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>25</td>
    <td>sla-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>26</td>
    <td>ssc-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>27</td>
    <td>tgu-miR-34a</td>
    <td>134</td>
    <td>76</td>
    <td>189</td>
    <td>56</td>
    <td>75</td>
  </tr>
  <tr>
    <td>28</td>
    <td>prd-let-7-5p</td>
    <td>283</td>
    <td>201</td>
    <td>540</td>
    <td>169</td>
    <td>186</td>
  </tr>
  <tr>
    <td>29</td>
    <td>abu-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>30</td>
    <td>aca-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>31</td>
    <td>ami-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>32</td>
    <td>asu-let-7-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>33</td>
    <td>bbe-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>34</td>
    <td>bfl-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>35</td>
    <td>bma-let-7</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>36</td>
    <td>bta-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>37</td>
    <td>cbn-let-7</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>38</td>
    <td>cbr-let-7</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>39</td>
    <td>ccr-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>40</td>
    <td>cel-let-7-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>41</td>
    <td>cfa-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>42</td>
    <td>cgr-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>43</td>
    <td>chi-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>44</td>
    <td>cja-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>45</td>
    <td>cli-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>46</td>
    <td>cpi-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
</table>
<table>
  <tr>
    <td>1</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>2</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>3</td>
    <td>cpo-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>4</td>
    <td>crm-let-7</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>5</td>
    <td>dma-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>6</td>
    <td>dno-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>7</td>
    <td>dre-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>8</td>
    <td>eca-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>9</td>
    <td>fru-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>10</td>
    <td>gga-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>11</td>
    <td>gga-let-7j-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>12</td>
    <td>gmo-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>13</td>
    <td>hpo-let-7-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>14</td>
    <td>hsa-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>15</td>
    <td>ipu-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>16</td>
    <td>lgi-let-7</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>17</td>
    <td>mdo-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>18</td>
    <td>mml-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>19</td>
    <td>mmr-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>20</td>
    <td>mmu-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>21</td>
    <td>mze-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>22</td>
    <td>nbr-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>23</td>
    <td>nle-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>24</td>
    <td>oar-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>25</td>
    <td>ocu-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>26</td>
    <td>oga-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>27</td>
    <td>oha-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>28</td>
    <td>oha-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>29</td>
    <td>ola-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>30</td>
    <td>oni-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>31</td>
    <td>pal-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>32</td>
    <td>pbv-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>33</td>
    <td>pha-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>34</td>
    <td>pma-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>35</td>
    <td>pny-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>36</td>
    <td>ppa-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>37</td>
    <td>ppc-let-7</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>38</td>
    <td>ppy-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>39</td>
    <td>pte-let-7-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>40</td>
    <td>ptr-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>41</td>
    <td>rno-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>42</td>
    <td>sbo-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>43</td>
    <td>sko-let-7</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>44</td>
    <td>ssa-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>45</td>
    <td>ssc-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
</table>
<table>
  <tr>
    <td>1</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>2</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>3</td>
    <td>tgu-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>4</td>
    <td>tni-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>5</td>
    <td>xla-let-7a-5p</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>6</td>
    <td>xtr-let-7a</td>
    <td>276</td>
    <td>195</td>
    <td>528</td>
    <td>165</td>
    <td>184</td>
  </tr>
  <tr>
    <td>7</td>
    <td>abu-miR-429b</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>8</td>
    <td>aca-miR-429-3p</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>9</td>
    <td>bta-miR-429</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>10</td>
    <td>ccr-miR-429</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>11</td>
    <td>cfa-miR-429</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>12</td>
    <td>cgr-miR-429</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>13</td>
    <td>cli-miR-429-3p</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>14</td>
    <td>cpi-miR-429-3p</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>15</td>
    <td>cpo-miR-429-3p</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>16</td>
    <td>dno-miR-429-3p</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>17</td>
    <td>dre-miR-429a</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>18</td>
    <td>efu-miR-429</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>19</td>
    <td>fru-miR-429</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>20</td>
    <td>gga-miR-429-3p</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>21</td>
    <td>gmo-miR-429-3p</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>22</td>
    <td>mmu-miR-429-3p</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>23</td>
    <td>mze-miR-429b</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>24</td>
    <td>nbr-miR-429b</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>25</td>
    <td>oan-miR-429-3p</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>26</td>
    <td>oha-miR-429-3p</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>27</td>
    <td>oni-miR-429b</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>28</td>
    <td>pal-miR-429-3p</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>29</td>
    <td>pbv-miR-429-3p</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>30</td>
    <td>pyy-miR-429b</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>31</td>
    <td>rno-miR-429</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>32</td>
    <td>ssc-miR-429</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>33</td>
    <td>tni-miR-429</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>34</td>
    <td>xla-miR-429-3p</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>35</td>
    <td>xtr-miR-429</td>
    <td>9</td>
    <td>37</td>
    <td>167</td>
    <td>23</td>
    <td>39</td>
  </tr>
  <tr>
    <td>36</td>
    <td>aof-miR166c</td>
    <td>101</td>
    <td>1840</td>
    <td>1341</td>
    <td>461</td>
    <td>530</td>
  </tr>
  <tr>
    <td>37</td>
    <td>ath-miR165a-3p</td>
    <td>101</td>
    <td>1840</td>
    <td>1341</td>
    <td>461</td>
    <td>530</td>
  </tr>
  <tr>
    <td>38</td>
    <td>ath-miR165b</td>
    <td>101</td>
    <td>1840</td>
    <td>1341</td>
    <td>461</td>
    <td>530</td>
  </tr>
  <tr>
    <td>39</td>
    <td>bna-miR166f</td>
    <td>101</td>
    <td>1840</td>
    <td>1341</td>
    <td>461</td>
    <td>530</td>
  </tr>
  <tr>
    <td>40</td>
    <td>cas-miR165a</td>
    <td>101</td>
    <td>1840</td>
    <td>1341</td>
    <td>461</td>
    <td>530</td>
  </tr>
  <tr>
    <td>41</td>
    <td>lus-miR166b</td>
    <td>101</td>
    <td>1840</td>
    <td>1341</td>
    <td>461</td>
    <td>530</td>
  </tr>
  <tr>
    <td>42</td>
    <td>cli-let-7i-5p</td>
    <td>203</td>
    <td>105</td>
    <td>205</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>43</td>
    <td>abu-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>44</td>
    <td>aca-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>45</td>
    <td>bta-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>46</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>47</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>48</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>49</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>50</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>51</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>52</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>53</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>54</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>55</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>56</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>57</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>58</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>59</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>60</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
</table>
<table>
  <tr>
    <td>1</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>2</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>3</td>
    <td>chi-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>4</td>
    <td>cja-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>5</td>
    <td>cpi-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>6</td>
    <td>cpo-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>7</td>
    <td>dno-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>8</td>
    <td>dre-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>9</td>
    <td>fru-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>10</td>
    <td>gmo-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>11</td>
    <td>hsa-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>12</td>
    <td>ipu-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>13</td>
    <td>mml-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>14</td>
    <td>mmu-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>15</td>
    <td>mze-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>16</td>
    <td>nbr-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>17</td>
    <td>nle-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>18</td>
    <td>oar-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>19</td>
    <td>ocu-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>20</td>
    <td>oga-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>21</td>
    <td>oha-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>22</td>
    <td>oni-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>23</td>
    <td>pal-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>24</td>
    <td>pbv-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>25</td>
    <td>pha-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>26</td>
    <td>pny-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>27</td>
    <td>ppa-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>28</td>
    <td>ppy-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>29</td>
    <td>ptr-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>30</td>
    <td>rno-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>31</td>
    <td>ssa-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>32</td>
    <td>tch-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>33</td>
    <td>tgu-let-7i-5p</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>34</td>
    <td>tni-let-7i</td>
    <td>201</td>
    <td>103</td>
    <td>204</td>
    <td>74</td>
    <td>111</td>
  </tr>
  <tr>
    <td>35</td>
    <td>ami-miR-30d-5p</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>36</td>
    <td>bta-miR-30d</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>37</td>
    <td>cfa-miR-30d</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>38</td>
    <td>cli-miR-30d-5p</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>39</td>
    <td>cpo-miR-30d-5p</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>40</td>
    <td>dno-miR-30d-5p</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>41</td>
    <td>gmo-miR-30a-5p</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>42</td>
    <td>nle-miR-30d</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>43</td>
    <td>ocu-miR-30d-5p</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>44</td>
    <td>oga-miR-30d</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>45</td>
    <td>oha-miR-30d-5p</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>46</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>47</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>48</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>49</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>50</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>51</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>52</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>53</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>54</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>55</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>56</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>57</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>58</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>59</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>60</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
</table>
<table>
  <tr>
    <td>1</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>2</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>3</td>
    <td>pal-miR-30d-5p</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>4</td>
    <td>pbv-miR-30d-5p</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>5</td>
    <td>pha-miR-30d</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>6</td>
    <td>ssa-miR-30b-5p</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>7</td>
    <td>ssc-miR-30d</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>8</td>
    <td>tgu-miR-30d-5p</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>9</td>
    <td>xla-miR-30d-5p</td>
    <td>75</td>
    <td>82</td>
    <td>136</td>
    <td>31</td>
    <td>60</td>
  </tr>
  <tr>
    <td>10</td>
    <td>cgr-miR-29a-3p</td>
    <td>88</td>
    <td>63</td>
    <td>108</td>
    <td>32</td>
    <td>67</td>
  </tr>
  <tr>
    <td>11</td>
    <td>chi-miR-29a-3p</td>
    <td>88</td>
    <td>63</td>
    <td>108</td>
    <td>32</td>
    <td>67</td>
  </tr>
  <tr>
    <td>12</td>
    <td>oar-miR-29a</td>
    <td>88</td>
    <td>63</td>
    <td>108</td>
    <td>32</td>
    <td>67</td>
  </tr>
  <tr>
    <td>13</td>
    <td>tch-miR-29a-3p</td>
    <td>88</td>
    <td>63</td>
    <td>108</td>
    <td>32</td>
    <td>67</td>
  </tr>
  <tr>
    <td>14</td>
    <td>ssa-miR-23b-3p</td>
    <td>101</td>
    <td>31</td>
    <td>58</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>15</td>
    <td>ami-miR-23b-3p</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>16</td>
    <td>bta-miR-10174-3p</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>17</td>
    <td>bta-miR-23b-3p</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>18</td>
    <td>cpo-miR-23b-3p</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>19</td>
    <td>dno-miR-23b-3p</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>20</td>
    <td>dre-miR-23b-3p</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>21</td>
    <td>efu-miR-23b</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>22</td>
    <td>hsa-miR-23b-3p</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>23</td>
    <td>ocu-miR-23b-3p</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>24</td>
    <td>oga-miR-23b</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>25</td>
    <td>oha-miR-23b-3p</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>26</td>
    <td>ola-miR-23b</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>27</td>
    <td>pbv-miR-23b-3p</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>28</td>
    <td>pma-miR-23b</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>29</td>
    <td>ppa-miR-23b</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>30</td>
    <td>ppy-miR-23b</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>31</td>
    <td>ptr-miR-23b</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>32</td>
    <td>tch-miR-23b-3p</td>
    <td>98</td>
    <td>30</td>
    <td>57</td>
    <td>21</td>
    <td>45</td>
  </tr>
  <tr>
    <td>33</td>
    <td>cfa-miR-29a</td>
    <td>117</td>
    <td>82</td>
    <td>137</td>
    <td>45</td>
    <td>82</td>
  </tr>
  <tr>
    <td>34</td>
    <td>cja-miR-29a</td>
    <td>117</td>
    <td>82</td>
    <td>137</td>
    <td>45</td>
    <td>82</td>
  </tr>
  <tr>
    <td>35</td>
    <td>cpo-miR-29a-3p</td>
    <td>117</td>
    <td>82</td>
    <td>137</td>
    <td>45</td>
    <td>82</td>
  </tr>
  <tr>
    <td>36</td>
    <td>dma-miR-29a</td>
    <td>117</td>
    <td>82</td>
    <td>137</td>
    <td>45</td>
    <td>82</td>
  </tr>
  <tr>
    <td>37</td>
    <td>dno-miR-29a-3p</td>
    <td>117</td>
    <td>82</td>
    <td>137</td>
    <td>45</td>
    <td>82</td>
  </tr>
  <tr>
    <td>38</td>
    <td>eca-miR-29a</td>
    <td>117</td>
    <td>82</td>
    <td>137</td>
    <td>45</td>
    <td>82</td>
  </tr>
  <tr>
    <td>39</td>
    <td>hsa-miR-29a-3p</td>
    <td>117</td>
    <td>82</td>
    <td>137</td>
    <td>45</td>
    <td>82</td>
  </tr>
  <tr>
    <td>40</td>
    <td>mmr-miR-29a</td>
    <td>117</td>
    <td>82</td>
    <td>137</td>
    <td>45</td>
    <td>82</td>
  </tr>
  <tr>
    <td>41</td>
    <td>mmu-miR-29a-3p</td>
    <td>117</td>
    <td>82</td>
    <td>137</td>
    <td>45</td>
    <td>82</td>
  </tr>
  <tr>
    <td>42</td>
    <td>nle-miR-29a</td>
    <td>117</td>
    <td>82</td>
    <td>137</td>
    <td>45</td>
    <td>82</td>
  </tr>
  <tr>
    <td>43</td>
    <td>ocu-miR-29a-3p</td>
    <td>117</td>
    <td>82</td>
    <td>137</td>
    <td>45</td>
    <td>82</td>
  </tr>
  <tr>
    <td>44</td>
    <td>oga-miR-29a</td>
    <td>117</td>
    <td>82</td>
    <td>137</td>
    <td>45</td>
    <td>82</td>
  </tr>
  <tr>
    <td>45</td>
    <td>pal-miR-29a-3p</td>
    <td>117</td>
    <td>82</td>
    <td>137</td>
    <td>45</td>
    <td>82</td>
  </tr>
  <tr>
    <td>46</td>
    <td>1</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>47</td>
    <td>2</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>48</td>
    <td>3</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>49</td>
    <td>4</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>50</td>
    <td>5</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>51</td>
    <td>6</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>52</td>
    <td>7</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>53</td>
    <td>8</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>54</td>
    <td>9</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>55</td>
    <td>10</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>56</td>
    <td>11</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>57</td>
    <td>12</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>58</td>
    <td>13</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>59</td>
    <td>14</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>60</td>
    <td>15</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
</table>
<table>
  <tr>
    <td>1</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>2</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>3</td>
    <td>pha-miR-29a</td>
    <td>117</td>
    <td>82</td>
    <td>137</td>
    <td>45</td>
    <td>82</td>
  </tr>
  <tr>
    <td>4</td>
    <td>rno-miR-29a-3p</td>
    <td>117</td>
    <td>82</td>
    <td>137</td>
    <td>45</td>
    <td>82</td>
  </tr>
  <tr>
    <td>5</td>
    <td>cli-miR-23b-3p</td>
    <td>99</td>
    <td>30</td>
    <td>57</td>
    <td>22</td>
    <td>45</td>
  </tr>
  <tr>
    <td>6</td>
    <td>age-miR-29a</td>
    <td>170</td>
    <td>93</td>
    <td>148</td>
    <td>58</td>
    <td>108</td>
  </tr>
  <tr>
    <td>7</td>
    <td>ggo-miR-29a</td>
    <td>170</td>
    <td>93</td>
    <td>148</td>
    <td>58</td>
    <td>108</td>
  </tr>
  <tr>
    <td>8</td>
    <td>lla-miR-29a</td>
    <td>170</td>
    <td>93</td>
    <td>148</td>
    <td>58</td>
    <td>108</td>
  </tr>
  <tr>
    <td>9</td>
    <td>mml-miR-29a-3p</td>
    <td>170</td>
    <td>93</td>
    <td>148</td>
    <td>58</td>
    <td>108</td>
  </tr>
  <tr>
    <td>10</td>
    <td>mne-miR-29a</td>
    <td>170</td>
    <td>93</td>
    <td>148</td>
    <td>58</td>
    <td>108</td>
  </tr>
  <tr>
    <td>11</td>
    <td>ppa-miR-29a</td>
    <td>170</td>
    <td>93</td>
    <td>148</td>
    <td>58</td>
    <td>108</td>
  </tr>
  <tr>
    <td>12</td>
    <td>ppy-miR-29a</td>
    <td>170</td>
    <td>93</td>
    <td>148</td>
    <td>58</td>
    <td>108</td>
  </tr>
  <tr>
    <td>13</td>
    <td>ptr-miR-29a</td>
    <td>170</td>
    <td>93</td>
    <td>148</td>
    <td>58</td>
    <td>108</td>
  </tr>
  <tr>
    <td>14</td>
    <td>sbo-miR-29a</td>
    <td>170</td>
    <td>93</td>
    <td>148</td>
    <td>58</td>
    <td>108</td>
  </tr>
  <tr>
    <td>15</td>
    <td>sla-miR-29a</td>
    <td>170</td>
    <td>93</td>
    <td>148</td>
    <td>58</td>
    <td>108</td>
  </tr>
  <tr>
    <td>16</td>
    <td>gga-miR-126-3p</td>
    <td>752</td>
    <td>501</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>21</td>
    <td>bta-miR-200c</td>
    <td>2</td>
    <td>59</td>
    <td>226</td>
    <td>29</td>
    <td>53</td>
  </tr>
  <tr>
    <td>22</td>
    <td>cfa-miR-200c</td>
    <td>2</td>
    <td>59</td>
    <td>226</td>
    <td>29</td>
    <td>53</td>
  </tr>
  <tr>
    <td>23</td>
    <td>cgr-miR-200c</td>
    <td>2</td>
    <td>59</td>
    <td>226</td>
    <td>29</td>
    <td>53</td>
  </tr>
  <tr>
    <td>24</td>
    <td>chi-miR-200c</td>
    <td>2</td>
    <td>59</td>
    <td>226</td>
    <td>29</td>
    <td>53</td>
  </tr>
  <tr>
    <td>25</td>
    <td>cja-miR-200c</td>
    <td>2</td>
    <td>59</td>
    <td>226</td>
    <td>29</td>
    <td>53</td>
  </tr>
  <tr>
    <td>26</td>
    <td>cpo-miR-200c-3p</td>
    <td>2</td>
    <td>59</td>
    <td>226</td>
    <td>29</td>
    <td>53</td>
  </tr>
  <tr>
    <td>27</td>
    <td>eca-miR-200c</td>
    <td>2</td>
    <td>59</td>
    <td>226</td>
    <td>29</td>
    <td>53</td>
  </tr>
  <tr>
    <td>28</td>
    <td>efu-miR-200c</td>
    <td>2</td>
    <td>59</td>
    <td>226</td>
    <td>29</td>
    <td>53</td>
  </tr>
  <tr>
    <td>29</td>
    <td>hsa-miR-200c-3p</td>
    <td>2</td>
    <td>59</td>
    <td>226</td>
    <td>29</td>
    <td>53</td>
  </tr>
  <tr>
    <td>30</td>
    <td>mmu-miR-200c-3p</td>
    <td>2</td>
    <td>59</td>
    <td>226</td>
    <td>29</td>
    <td>53</td>
  </tr>
  <tr>
    <td>31</td>
    <td>ocu-miR-200c-3p</td>
    <td>2</td>
    <td>59</td>
    <td>226</td>
    <td>29</td>
    <td>53</td>
  </tr>
  <tr>
    <td>32</td>
    <td>pal-miR-200c-3p</td>
    <td>2</td>
    <td>59</td>
    <td>226</td>
    <td>29</td>
    <td>53</td>
  </tr>
  <tr>
    <td>33</td>
    <td>ptr-miR-200c</td>
    <td>2</td>
    <td>59</td>
    <td>226</td>
    <td>29</td>
    <td>53</td>
  </tr>
  <tr>
    <td>34</td>
    <td>tch-miR-200c-3p</td>
    <td>2</td>
    <td>59</td>
    <td>226</td>
    <td>29</td>
    <td>53</td>
  </tr>
  <tr>
    <td>35</td>
    <td>aca-miR-126-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>40</td>
    <td>ami-miR-126-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>41</td>
    <td>cgr-miR-126a</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>42</td>
    <td>cja-miR-126</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>43</td>
    <td>cli-miR-126-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>44</td>
    <td>cpi-miR-126-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>45</td>
    <td>cpo-miR-126-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>46</td>
    <td>dno-miR-126-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>47</td>
    <td>eca-miR-126-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>48</td>
    <td>hsa-miR-126-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>49</td>
    <td>mdo-miR-126-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>50</td>
    <td>mml-miR-126</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>51</td>
    <td>mmu-miR-126a-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>52</td>
    <td>oan-miR-126-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>53</td>
    <td>pbv-miR-126-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>54</td>
    <td>mdo-miR-126-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>55</td>
    <td>mml-miR-126</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>56</td>
    <td>mmu-miR-126a-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>57</td>
    <td>oan-miR-126-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>58</td>
    <td>pbv-miR-126-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
  </tr>
  <tr>
    <td>59</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>60</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
</table>
<table>
  <tr>
    <td>1</td>
    <td>2</td>
    <td>3</td>
    <td>4</td>
    <td>5</td>
    <td>6</td>
    <td>7</td>
    <td>8</td>
    <td>9</td>
    <td>10</td>
    <td>11</td>
    <td>12</td>
    <td>13</td>
    <td>14</td>
    <td>15</td>
    <td>16</td>
    <td>17</td>
    <td>18</td>
    <td>19</td>
    <td>20</td>
    <td>21</td>
    <td>22</td>
    <td>23</td>
    <td>24</td>
    <td>25</td>
    <td>26</td>
    <td>27</td>
    <td>28</td>
    <td>29</td>
    <td>30</td>
    <td>31</td>
    <td>32</td>
    <td>33</td>
    <td>34</td>
    <td>35</td>
    <td>36</td>
    <td>37</td>
    <td>38</td>
    <td>39</td>
    <td>40</td>
    <td>41</td>
    <td>42</td>
    <td>43</td>
    <td>44</td>
    <td>45</td>
    <td>46</td>
    <td>47</td>
    <td>48</td>
    <td>49</td>
    <td>50</td>
    <td>51</td>
    <td>52</td>
    <td>53</td>
    <td>54</td>
    <td>55</td>
    <td>56</td>
    <td>57</td>
    <td>58</td>
    <td>59</td>
    <td>60</td>
  </tr>
  <tr>
    <td>ppy-miR-126</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
    <td>ptr-miR-126</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
    <td>rno-miR-126a-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
    <td>ssc-miR-126-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
    <td>tgu-miR-126-3p</td>
    <td>752</td>
    <td>499</td>
    <td>671</td>
    <td>291</td>
    <td>487</td>
    <td>ccr-miR-30d</td>
    <td>46</td>
    <td>49</td>
    <td>94</td>
    <td>18</td>
    <td>34</td>
    <td>cja-miR-30d</td>
    <td>46</td>
    <td>49</td>
    <td>94</td>
    <td>18</td>
    <td>34</td>
    <td>cpi-miR-30d-5p</td>
    <td>46</td>
    <td>49</td>
    <td>94</td>
    <td>18</td>
    <td>34</td>
    <td>oan-miR-30d-5p</td>
    <td>46</td>
    <td>49</td>
    <td>94</td>
    <td>18</td>
    <td>34</td>
    <td>ola-miR-30d-5p</td>
    <td>46</td>
    <td>49</td>
    <td>94</td>
    <td>18</td>
    <td>34</td>
    <td>efu-miR-126</td>
    <td>756</td>
    <td>506</td>
    <td>678</td>
    <td>291</td>
    <td>498</td>
    <td>ccr-miR-100</td>
    <td>126</td>
    <td>30</td>
    <td>25</td>
    <td>38</td>
    <td>32</td>
    <td>cgr-miR-100-5p</td>
    <td>126</td>
    <td>30</td>
    <td>25</td>
    <td>38</td>
    <td>32</td>
    <td>chi-miR-100-5p</td>
    <td>126</td>
    <td>30</td>
    <td>25</td>
    <td>38</td>
    <td>32</td>
    <td>cpi-miR-100-5p</td>
    <td>126</td>
    <td>30</td>
    <td>25</td>
    <td>38</td>
    <td>32</td>
    <td>pmi-miR-100-5p</td>
    <td>126</td>
    <td>30</td>
    <td>25</td>
    <td>38</td>
    <td>32</td>
    <td>dma-miR-100</td>
    <td>126</td>
    <td>30</td>
    <td>25</td>
    <td>38</td>
    <td>32</td>
    <td>mdo-miR-100-5p</td>
    <td>126</td>
    <td>30</td>
    <td>25</td>
    <td>38</td>
    <td>32</td>
    <td>sbo-miR-100</td>
    <td>126</td>
    <td>30</td>
    <td>25</td>
    <td>38</td>
    <td>32</td>
    <td>sha-miR-100</td>
    <td>126</td>
    <td>30</td>
    <td>25</td>
    <td>38</td>
    <td>32</td>
    <td>tgu-miR-100-5p</td>
    <td>126</td>
    <td>30</td>
    <td>25</td>
    <td>38</td>
    <td>32</td>
    <td>efu-miR-29a</td>
    <td>207</td>
    <td>117</td>
    <td>182</td>
    <td>73</td>
    <td>128</td>
    <td>gga-let-7g-5p</td>
    <td>27</td>
    <td>68</td>
    <td>130</td>
    <td>39</td>
    <td>56</td>
    <td>ggo-let-7g</td>
    <td>27</td>
    <td>68</td>
    <td>130</td>
    <td>39</td>
    <td>56</td>
    <td>mdo-let-7g-5p</td>
    <td>27</td>
    <td>68</td>
    <td>130</td>
    <td>39</td>
    <td>56</td>
    <td>oar-let-7g</td>
    <td>27</td>
    <td>68</td>
    <td>130</td>
    <td>39</td>
    <td>56</td>
    <td>sha-let-7g</td>
    <td>27</td>
    <td>68</td>
    <td>130</td>
    <td>39</td>
    <td>56</td>
    <td>bta-miR-29a</td>
    <td>200</td>
    <td>114</td>
    <td>179</td>
    <td>73</td>
    <td>124</td>
    <td>ssc-miR-29a-3p</td>
    <td>200</td>
    <td>114</td>
    <td>179</td>
    <td>73</td>
    <td>124</td>
    <td>cpo-miR-16b-5p</td>
    <td>401</td>
    <td>204</td>
    <td>217</td>
    <td>133</td>
    <td>202</td>
    <td>dno-miR-16b-5p</td>
    <td>401</td>
    <td>204</td>
    <td>217</td>
    <td>133</td>
    <td>202</td>
    <td>efu-miR-16</td>
    <td>401</td>
    <td>204</td>
    <td>217</td>
    <td>133</td>
    <td>202</td>
    <td>ocu-miR-16b-5p</td>
    <td>401</td>
    <td>204</td>
    <td>217</td>
    <td>133</td>
    <td>202</td>
    <td>bta-miR-375</td>
    <td>12</td>
    <td>470</td>
    <td>1593</td>
    <td>199</td>
    <td>498</td>
    <td>efu-miR-375</td>
    <td>12</td>
    <td>470</td>
    <td>1593</td>
    <td>199</td>
    <td>498</td>
    <td>mdo-miR-375</td>
    <td>12</td>
    <td>470</td>
    <td>1593</td>
    <td>199</td>
    <td>498</td>
    <td>cfa-miR-375</td>
    <td>12</td>
    <td>464</td>
    <td>1564</td>
    <td>196</td>
    <td>491</td>
    <td>cpo-miR-375-3p</td>
    <td>12</td>
    <td>464</td>
    <td>1564</td>
    <td>196</td>
    <td>491</td>
    <td>dno-miR-375-3p</td>
    <td>12</td>
    <td>464</td>
    <td>1564</td>
    <td>196</td>
    <td>491</td>
    <td>ggo-miR-375</td>
    <td>12</td>
    <td>464</td>
    <td>1564</td>
    <td>196</td>
    <td>491</td>
    <td>hsa-miR-375-3p</td>
    <td>12</td>
    <td>464</td>
    <td>1564</td>
    <td>196</td>
    <td>491</td>
    <td>mml-miR-375</td>
    <td>12</td>
    <td>464</td>
    <td>1564</td>
    <td>196</td>
    <td>491</td>
    <td>mmu-miR-375-3p</td>
    <td>12</td>
    <td>464</td>
    <td>1564</td>
    <td>196</td>
    <td>491</td>
  </tr>
</table>