# Differential apple gene expression during interaction of Erwinia amylovora with a resistant and a susceptible genotype

Jean-Stéphane Venisse, Isabelle Pontais, David Lalanne, Jean-Pierre Paulin, Marie-Noelle Brisset

► To cite this version:

Jean-Stéphane Venisse, Isabelle Pontais, David Lalanne, Jean-Pierre Paulin, Marie-Noelle Brisset. Differential apple gene expression during interaction of Erwinia amylovora with a resistant and a susceptible genotype. 10.International Congress of Molecular Plant Microbe Interaction, Jul 2001, Madison, United States. 2001, Proceedings of the X International Congress of Molecular Plant Microbe Interaction. hal-01600998

HAL Id: hal-01600998

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Submitted on 4 Jun 2020

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# Differential apple gene expression during interaction of Erwinia amylovora with a resistant and a susceptible genotype

Venisse J.S., Pontais I., Lalanne D., Paulin J.P. and Brisset M.N.

UMR Pathologie Végétale INRA/INH/UA and Unité d'Amélioration des Plantes INRA, Centre d'Angers,
42 rue Georges Morel, BP 57, 49071 Beaucouzé Cedex, France

Erwinia amylovora is the causal agent of fire blight of Maloideae (apple, pear). In order to identify mechanisms which discriminate incompatible from compatible interactions, differential gene expression was undertaken through cDNA-AFLP in a resistant (R) and a susceptible (S) apple genotypes challenged with a virulent wild-type strain (Ea wt). Expression patterns were compared with those obtained from the same genotypes of apple challenged with an avirulent hrp secretory mutant of E. amylovora (Ea hrp) and with the tobacco pathogen Pseudomonas syringae pv. tabaci (Pst wt), both used as controls. Three major classes of genes were identified and time-course of their expression was analyzed by semi-quantitative RT-PCR.

## 1 - Early induced genes in the resistant genotype (R)

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## 2 - Early induced genes in the susceptible genotype (S)

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## 3 - Early repressed genes in the susceptible genotype (S)

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INRA