Sep 22, 2019

Version 2

LB Agar (1 Liter) V.2

DOI

https://dx.doi.org/10.17504/protocols.io.7jvhkn6

Alba Balletbó¹

¹Wageningen University

iGEM Wageningen 2019

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Alba Balletbó

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DOI: https://dx.doi.org/10.17504/protocols.io.7jvhkn6

**Protocol Citation:** Alba Balletbó 2019. LB Agar (1 Liter). protocols.io https://dx.doi.org/10.17504/protocols.io.7jvhkn6

**License:** This is an open access protocol distributed under the terms of the **Creative Commons Attribution License**, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

**Protocol status:** Working

We use this protocol and it's working
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**Created:** September 22, 2019

**Last Modified:** September 22, 2019

**Protocol Integer ID:** 27989

**Keywords:** lb agar, liter

## Guidelines

Mixture must be autoclaved before use.

## Materials

### MATERIALS

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Distilled Water

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Agar

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NaCl Merck MilliporeSigma (Sigma-Aldrich) Catalog #53014

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Tryptone Fisher Scientific Catalog #BP1421-500

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Yeast Extract Thermo Fisher Catalog #211930

## Troubleshooting
1. Add LB agar components in a 1 L autoclavable. LB agar components (1 L):

<table><tr><td>NaCl</td><td>10<br>gr<br>m</td></tr><tr><td>Tryp<br>tone</td><td>10<br>gr<br>m</td></tr><tr><td>Yeas<br>t<br>Extr<br>act</td><td>5<br>gr<br>m</td></tr><tr><td>Agar</td><td>15<br>gr<br>m</td></tr></table>

2. Add 1 L of distilled water into the bottle.

3. Vigorously shake the bottle.

4. Place the bottle into the autoclave and sterilize.