Composition of powdered aureobacidium culture solution, manufacturing method thereof and powder mixture with the composition

Disclosed is a mixture excellent in coagulation of fats and oils together with a method of powdering the Aureobacidium culture solution. Formation of a powdered clathrate that is formed by including the Aureobacidium culture solution as a guest component into cyclodextrin as a host component improves its physical properties and shelf life. Preferably, chitosan is added to the above-described composition of the powdered Aureobacidium culture solution.

EXAMPLE 1 Mixture of 700 parts by weight of a commercially available Aureobacidium culture solution (“RAKUSHO”TM: from Health Support Japan Corporation: Tokyo) and 300 parts by weight of cyclodextrin (“K-100”TM: from Ensuiko Sugar Refining Co., Ltd.) which contains about 70 wt % &agr;-cyclodextrin by weight was mixed with 1000 parts by weight of water and stirred vigorously with a homogenizer. The stirring condition was set at 4000 rpm for the first 10 minutes and 2000 rpm for the subsequent 15 minutes. Through this stirring process, clathration was performed containing the Aureobacidium culture solution as a guest into the central cavity of cyclodextrin as a host. After the stirring process was finished, the liquid mixture was spray-dried with a spray dryer and the like at about 170° C. and a composition of powdered Aureobacidium culture solution was obtained. The powder showed a sufficient coagulating effect when it was used together with aluminum sulfate. Next, experiments on powders in the case where the Aureobacidium culture solution was mixed with &agr;-CD, &bgr;-CD and &ggr;-CD respectively were carried out. Each powder in which &agr;-CD, &bgr;-CD and &ggr;-CD were used under the same conditions as those of the above-described EXAMPLE 1 respectively was used together with aluminum sulfate and examined whether or not coagulating function was achieved. The result is shown in TABLE 1. In TABLE 1, “G” for “Good” represents the case where coagulating function was recognized, and “NG” for “Not Good” represents the case where the coagulation function was slightly recognized, but not remarkably. 1 TABLE 1 Aureobacidium culture solution &agr;-CD &bgr;-CD &ggr;-CD Evaluation of powdering G NG NG Then, &agr;-CD was mixed with the mixture of &bgr;-CD and &ggr;-CD (1:1) and coagulating property in the case where only the content ratio of &agr;-CD was varied was examined. The result is shown in TABLE 2. 2 TABLE 2 &agr;-CD (&bgr;-CD) &plus; (&ggr;-CD) Evaluation of powdering 10(wt %) 90(wt %) NG 30 70 NG 50 50 G 70 30 G 80 20 G According to the above results, powdering with good coagulation is made possible when the Aureobacidium culture solution is mixed with cyclodextrin containing 50 wt % or more &agr;-CD. Stability Test (1 ) Right after Production In order to check the stability holding of compositions of the Aureobacidium culture solution in the form of powdered clathration produced by the method of the present invention, the property of rapid coagulation (gelling) by aluminum ion in the culture solution described in the above-mentioned Patent Office Gazette was utilized. 20 parts by weight of the composition of powdered Aureobacidium culture solution (sample A) obtained by the present example was mixed with 100 parts of water, then stirred well to a state of solution. When 20 parts by weight of 25 wt % aqueous solution of aluminum sulfate was added herein, the solution gelled at once and turned into paste like “konjak” jelly. For comparison, the same test was repeated for 20 parts of the raw material of Aureobacidium culture solution (sample B: control) used for the present example, and the similar gelling was observed. Accordingly, the result shows that the culture solution contents are substantially held stable and do not substantially receive any change in quality like deterioration, degradation and so on through the treatments of clathration and spray drying due to the present invention. (2) After Storage The same samples A and B mentioned above were subjected to the same test described as in (1) above after they had stored as they were in wide-mouthed bottles being opened in a room from spring to summer, for three months from the middle of July to the middle of September (in Tokyo). The sample A of the present invention showed a gel formation with the hardness equal to that of a fresh one. As for the sample B, it started to become moldy on the surface after a week of storage, and became covered with mold over the whole surface giving out a nasty smell in two weeks. It was not a usable condition, and it was clear that the contents had changed in quality, deteriorated or degraded. As for the sample A based on the present invention, a slight growth of mold was observed on the surface portion just before the end of the examination period, but it did not extend all over, and no nasty smell generation was recognized. 
 EXAMPLE 2 The operation of EXAMPLE 1 was herein employed. In addition to the operation, the amounts of Aureobacidium culture solution and cyclodextrin used in EXAMPLE 1 were changed to 900 parts by weight and 100 parts by weight respectively, and ultrasonic of 90 kHz (1 kW nominal power) was applied during the latter part (2000 rpm for 15 minutes) of the stirring process. The composition obtained here gelled extremely hard in the stability test. Except for that, about the same result as that of EXAMPLE 1 in terms of the stability tests (1) and (2) as well as the tableting test was obtained. 
 EXAMPLE 3 (1) Coagulation Test of Fats and Oils 100 ml of water in a beaker was added with 3 mg of a Chinese chili oil and stirred well. Then each 10 mg of (i) Aureobacidium culture solution, (ii) cyclodextrin, (iii) powder of cyclodextrin clathrating Aureobacidium culture solution, and (iv) chitosan (product of Koyo Chemical Co., Ltd), was added as a simple material or a mixture of them and stirred. Then, states of coagulation of the chili oil were observed. Note that in the case of above mixture, the mixing ratio was set equal. Coagulation of the chili oil into a spherical shape with a diameter of 3 mm or more was evaluated as “G”, coagulated sphere with a diameter of more than 2 mm to less than 3 mm was evaluated as “M” for “Moderate”, and coagulated sphere with a diameter of less than 2 mm was evaluated as “NG.” The results are shown in TABLE 3. The reason why the chili oil was used in the experiments here is that the chili oil has a red color and easy to confirm visually the existence of coagulation. 3 TABLE 3 Exp.1 Exp.2 Exp.3 Exp.4 Exp.5 Exp.6 Exp.7 Exp.8 Exp.9 (i) add add add add (ii) add add add add (iii) add add (iv) add add add add add Mark NG NG NG NG M M NG NG G Next, experiments on coagulation were conducted for a variety of mixing ratios of chitosan. In these experiments, coagulation was evaluated in the same manner as described above by varying only adding amount of the powder of cyclodextrin clathrating Aureobacidium culture solution (CD powder) for various values to 10 mg of chitosan. The results are shown in TABLE 4. 4 TABLE 4 Chito- 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 10.0 san(mg) CD 0.5 0.8 1.0 2.5 5.0 10.0 50.0 75.0 100 powder (mg) Mark NG M G G G G G M NG From the results above, it was proved that good coagulation of fats and oils could be obtained when chitosan was added to the powder of cyclodextrin clathrating Aureobacidium culture solution (CD powder) within the range of 10:1 to 1:5. Although cases of using the chili oil among fats and oils are shown in the above-mentioned examples of experiment, the present invention was able to get the same results in the cases of using other oils such as a sesame oil, a salad oil and so on. 
 Industrial Applicability The Aureobacidium culture solution composition according to the present invention has improved physical properties and shelf life in the form of powdered clathrations formed by treating the Aureobacidium culture solution as a guest component with cyclodextrin as a host component. Converting a viscous liquid of the usual Aureobacidium culture solution into a powder both improves the storability and handleability, and extends its application field and development area widely. Meanwhile, because the powder mixture of the composition of powdered Aureobacidium culture solution added with chitosan shows remarkable coagulation effect on fats and oils and chitosan is an edible matter, the mixture is also excellent in usefulness that it can be expected possibility of being used as a material of not only soap, cosmetics and the like but also for medicines, health foods and slimming foods.