Modified membrane spanning proteins and methods for the preparation and use thereof

In accordance with the present invention, there are provided functionally modulated tool receptors which are useful for drug discovery and development. In certain aspects and embodiments as described herein, a sophisticated and powerful approach has been designed that allows the rapid development of enhanced receptors, while simultaneously exploring millions of possibilities for improved properties with respect to such properties as protein expression, homogeneity, stabilization, conformational and activation pathway selectivity, antigenicity, immunogenicity, and the like. Indeed, the new methodology described herein represents a breakthrough by leveraging a full range of combinatorial amino acid replacements, in multiple positions simultaneously, in order to generate modified membrane-spanning proteins.

FIELD OF THE INVENTION

The present disclosure relates to methods to modulate the functional properties of membrane-spanning proteins. In one aspect, the present disclosure relates to modified membrane-spanning proteins. In another aspect, the present disclosure relates to methods for the use of such modified membrane-spanning proteins.

BACKGROUND OF THE INVENTION

The information provided herein and references cited are provided solely to assist the understanding of the reader, and does not constitute an admission that any of the references or information is prior art to the present invention.

Membrane-spanning proteins such as G Protein Coupled Receptors (GPCRs) are among the most important classes of drug targets, currently targeted by about 30% of therapeutics in the market. However, the use of GPCRs for basic drug discovery and development in the pharmaceutical industry is inherently difficult due to the notoriously low expression, homogeneity, and stability thereof, and the high conformational flexibility of these integral membrane proteins, especially when removed from the cellular environment. This has greatly limited the success of protein-based approaches such as small molecule screening/assays and structural determination for structure-guided drug discovery, as well as antibody discovery efforts. These limitations remain to be addressed.

Importantly, only a very limited number of therapeutic antibodies targeting GPCRs have been approved, while most marketed therapeutics are small molecules or small peptides. As experienced often with non-GPCR targets, biologic drugs such as antibodies may enable access to a therapeutic space where small molecule drugs have failed. Therefore, the availability of GPCR-targeting antibodies will potentially facilitate previously unattainable opportunities for the betterment of human health.

SUMMARY OF THE INVENTION

In accordance with the present invention, the limitations of the art have been addressed through the generation of stabilized tool receptors for use in drug discovery and development.

Therefore, in certain aspects and embodiments as described herein, a sophisticated and powerful approach has been designed that allows the rapid development of enhanced receptors, while simultaneously exploring millions of possibilities for improved properties, such as increased protein expression, increased protein stability, increased protein homogeneity, increased protein antigenicity, increased protein immunogenicity, increased protein crystallizability, modulated conformational selectivity, modulated activation pathway selectivity, and the like. Indeed, the new methodology described herein represents a breakthrough by leveraging a full range of combinatorial amino acid replacements, in multiple positions simultaneously, in order to generate modified membrane-spanning proteins.

In addition, the efficiency of the readout system employed herein, i.e., the system by which clones are identified, is exponentially improved over current screening methods. Therefore, besides achieving higher protein expression and trafficking, the resulting membrane-spanning proteins (e.g., GPCRs) are characterized by having a greater spectrum of variation with respect to properties such as, for example, higher stability, a higher degree of conformational selectivity, and a higher degree of homogeneity), and the like. Therefore, modified membrane-spanning proteins, as described herein have great potential to aid the development of both biologics, small peptides and small molecule drugs.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

In accordance with the present invention, there are provided methods to modulate the functional properties of a membrane-spanning protein, said methods comprising:generating a first polynucleotide library wherein a sufficient number of residues of a polynucleotide encoding said membrane-spanning protein are randomly modified so as to modulate the functional properties thereof,optionally generating a second polynucleotide library from said first polynucleotide library, said second polynucleotide library comprising a polynucleotide encoding said membrane-spanning protein modified by DNA shuffling in a sufficient number of amino acid residues so as to modulate the functional properties thereof,inserting the modified polynucleotides of said second polynucleotide library into a construct comprising:a signal sequence,a first marker sequence, wherein said first marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions and identifying the modified polynucleotide-containing construct(s) contained therein.

As used herein, the phrase “membrane-spanning protein” refers to plasma membrane proteins, nuclear membrane proteins, peripheral membrane proteins, intracellular-membrane proteins (e.g. mitochondrial membrane proteins), transporters, channels, adhesins, translocases and receptors like G Protein Coupled Receptors (GPCRs) from all classes (A-F). Exemplary G-protein coupled receptors (GPCR) include adhesion receptors, secretins, glutamates, frizzled/TAS2, rhodopsin, olfactory receptors, and the like. See, for example, the GPCR family tree set forth inFIG. 1.

In generating a first polynucleotide library wherein a sufficient number of residues of a polynucleotide encoding said membrane-spanning protein (and the corresponding polypeptide sequence after translation thereof) are randomly modified so as to modulate the functional properties thereof, one typically modifies in the range of 1-50 residues; in some embodiments, one typically modifies 1-40 residues; in some embodiments, one typically modifies 1-30 residues; in some embodiments, one typically modifies 1-20 residues; in some embodiments, one typically modifies 1-15 residues; in some embodiments, one typically modifies 2-50 residues; in some embodiments, one typically modifies 2-40 residues; in some embodiments, one typically modifies 2-30 residues; in some embodiments, one typically modifies 2-20 residues; in some embodiments, one typically modifies 2-15 residues; in some embodiments, one typically modifies 3-50 residues; in some embodiments, one typically modifies 3-40 residues; in some embodiments, one typically modifies 3-30 residues; in some embodiments, one typically modifies 3-20 residues; in some embodiments, one typically modifies 3-15 residues; in some embodiments, one typically modifies 4-50 residues; in some embodiments, one typically modifies 4-40 residues; in some embodiments, one typically modifies 4-30 residues; in some embodiments, one typically modifies 4-20 residues; in some embodiments, one typically modifies 4-15 residues; in some embodiments, one typically modifies 5-50 residues; in some embodiments, one typically modifies 5-40 residues; in some embodiments, one typically modifies 5-30 residues; in some embodiments, one typically modifies 5-20 residues; in some embodiments, one typically modifies 5-15 residues; an average of 6 residues are modified (mutated) per 1 kB of DNA; in some embodiments, an average of 8 residues are modified (mutated) per 1 kB of DNA; in some embodiments, an average of 10 residues are modified (mutated) per 1 kB of DNA; in some embodiments, an average of 12 residues are modified (mutated) per 1 kB of DNA.

In optionally generating a second polynucleotide library from said first polynucleotide library, said second polynucleotide library comprising a polynucleotide encoding said membrane-spanning protein modified by DNA shuffling in a sufficient number of amino acid residues so as to modulate the functional properties thereof, one typically modifies 1-50 residues; in some embodiments, one typically modifies 1-40 residues; in some embodiments, one typically modifies 1-30 residues; in some embodiments, one typically modifies 1-20 residues; in some embodiments, one typically modifies 1-15 different amino acid residues per polynucleotide. In some embodiments, one typically modifies 2-50 residues; in some embodiments, one typically modifies 2-40 residues; in some embodiments, one typically modifies 2-30 residues; in some embodiments, one typically modifies 2-20 residues; in some embodiments, one typically modifies 2-15 different amino acid residues per polynucleotide; in some embodiments, one typically modifies 3-50 residues; in some embodiments, one typically modifies 3-40 residues; in some embodiments, one typically modifies 3-30 residues; in some embodiments, one typically modifies 3-20 residues; in some embodiments, one typically modifies 3-15 different amino acid residues per polynucleotide; in some embodiments, one typically modifies 4-50 residues; in some embodiments, one typically modifies 4-40 residues; in some embodiments, one typically modifies 4-30 residues; in some embodiments, one typically modifies 4-20 residues; in some embodiments, one typically modifies 4-15 different amino acid residues per polynucleotide; in some embodiments, one typically modifies 5-50 residues; in some embodiments, one typically modifies 5-40 residues; in some embodiments, one typically modifies 5-30 residues; in some embodiments, one typically modifies 5-20 residues; in some embodiments, one typically modifies 5-15 different amino acid residues per polynucleotide.

As used herein, “signal sequence” refers to a sequence of amino acid residues in the amino terminus of a nascent protein during protein translation, which when recognized by the signal recognition particle results in the transport of the nascent protein via the translocation pathway of the host organism.

Marker sequences contemplated for use herein encode one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s) and/or selective pressure conditions. Exemplary marker sequences include ampicillin, chloramphenicol, neomycin, kanamycin, tetracycline, gentamicin resistance genes; pyrE gene (orotate phosphoribosyltransferase) and pyrF gene (orotidine-5′-monophosphate decarboxylase); URA3 gene (orotidine 5′-phosphate decarboxylase); LYS2 gene (alpha-aminoadipate reductase); ADE1-2 genes (phosphoribosylamino-imidazole-succinocarbozamide synthetase, phosphoribosylamino-imidazole-carboxylase), and the like.

Selective pressure agents/conditions refer to agents (including conditions) of differential mortality or fertility that tend to make a population change genetically. Exemplary selective pressure agents include antibiotics (e.g., ampicillin, carbenicillin, gentamicin, chloramphenicol, neomycin, kanamycin, tetracycline, and the like), toxic metabolites (e.g., 5-fluoroorotic acid or uracyl), conditions such as lack of nutrients for auxotrophic strains, and the like.

Exemplary selective pressure conditions include elevated temperature, reduced temperature, lack of necessary nutrient(s), co-factors, and the like. Exemplary nutrients include oxygen, carbon dioxide, and the like.

Other marker sequences contemplated for use herein encode reporter genes that confer traits that can be easily identified and measured in organisms expressing same. Exemplary marker sequences include beta-galactosidase, alkaline phosphatase, green fluorescent protein, red fluorescent protein, tdTomato fluorescent protein, luciferase, and the like.

Inserting genetic material into suitable host cells with the modified polynucleotide-containing constructs described herein can be carried out in a variety of ways, e.g., by transformation or transfection, the process by which nucleic acids are introduced into bacteria or mammalian cells, respectively. Protocols and techniques vary widely and include lipid transfection and chemical and physical methods such as electroporation.

A variety of techniques suitable to employ for selecting cells which survive exposure to the selective pressure agent(s)/conditions are well known in the art (e.g. cell growth on agar plates), as are methods for identifying the modified polynucleotide-containing construct(s) contained therein according to the present invention (e.g. the Sanger polynucleotide sequencing method).

In some embodiments of the present invention, the functional property(ies) of a membrane-spanning protein are selected from the expression level of said membrane-spanning protein, the stability of said membrane-spanning protein, the conformational selectivity of said membrane-spanning protein, the homogeneity of said membrane-spanning protein, the crystallizability of said membrane-spanning protein, the antigenicity of said membrane-spanning protein, the immunogenicity of said membrane-spanning protein, the activation pathway selectivity of said membrane-spanning protein, and the like.

In some embodiments of the present invention, additional assays are carried out to verify the occurrence of membrane-spanning proteins having modulated functional properties. Such verification can be carried out, for example, by:inserting the modified polynucleotide into suitable host cells,expressing said modified polynucleotide, andcharacterizing the resulting protein.

As readily recognized by those of skill in the art, a variety of characterization techniques and protocols are available to assess the various properties of the membrane proteins developed employing invention methods, e.g., homogeneity estimation and thermal denaturation assays, analytical size exclusion chromatography (SEC) or fluorescent SEC or fluorescent dyes; gel electrophoresis; direct binding measurements (including radioligand binding assays and surface plasmon resonance); signal transduction measurements (including cAMP production and calcium flux assays); and the like.

In some embodiments of the present invention, additional assays are carried out to identify the minimum working set of mutations required to achieve the desired modulated functional properties. Such evaluation can be carried out, for example, by:randomly reverting said modified residues back,re-selecting in the presence of selective pressure agent(s)/conditions (e.g., antibiotic-containing medium), andre-characterizing (the modulated functional properties) in order to identify the minimum working set of mutations.

As readily recognized by those of skill in the art, a variety of host cells are suitable for use in the invention methods. Exemplary host cells are typically characterized by one or more of the following criteria:being able to undergo transfection, transformation etc to receive (exogenous) genetic material (DNA, mRNA, plasmids, bacmids etc.);being able to receive a limited number of copies of genetic material;being able to form colonies or clonal biomass (genetically uniform);possessing one or more selection markers (e.g., antibiotics, auxotrophies, toxins);being able to effectively produce transmembrane proteins;possessing the appropriate cellular machinery for translocation of proteins to the membrane environment; and/orbeing able to grow in mesophilic, thermophilic, hyperthermophilic and/or other extreme conditions.

Suitable host cells contemplated for use herein can be selected from the group consisting of bacterial cell lines, yeast cell lines, insect cell lines, mammalian cell lines, and the like.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein.

In accordance with still another embodiment of the present invention, there are provided methods to modulate the functional properties of a membrane-spanning protein, said method comprising:generating a polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to modulate the functional properties thereof,inserting the modified polynucleotides of said second polynucleotide library into a construct comprising:a signal sequence, anda first marker sequence, wherein said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions,optionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein.

In accordance with yet another embodiment of the present invention, there are provided methods to modulate the functional properties of a membrane-spanning protein, said methods comprising:inserting each member of a modified polynucleotide library into a construct, wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to modulate the functional properties thereof, said construct comprising:a signal sequence, anda first marker sequence, wherein said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein.

In accordance with a further embodiment of the present invention, there are provided methods to modulate the functional properties of a membrane-spanning protein, said method comprising:transforming suitable host cells with a construct comprising:a modified polynucleotide,a signal sequence, andone or more marker sequences,wherein:said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions,said modified polynucleotide is obtained by generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to modulate the functional properties thereof, and optionally thereaftergenerating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein randomly modified in a sufficient number of amino acid residues so as to modulate the functional properties thereof, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein.

In accordance with yet another embodiment of the present invention, there are provided methods to modulate the functional properties of a membrane-spanning protein, said methods comprising selecting those cells which survive exposure to selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein, wherein said cells are transformed with:a modified polynucleotide,a signal sequence, andone or more marker sequences,wherein:said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions,said modified polynucleotide is obtained by generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to modulate the functional properties thereof, and optionally thereaftergenerating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein randomly modified in a sufficient number of amino acid residues so as to modulate the functional properties thereof.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein.

In certain aspects of the present invention, wherein the property to be modulated is the stability of said membrane-spanning protein, the invention method comprises:generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to modulate the stability thereof,optionally generating a second polynucleotide library from said first polynucleotide library, said second polynucleotide library comprising a polynucleotide encoding said membrane-spanning protein modified by DNA shuffling in a sufficient number of amino acid residues so as to modulate the stability thereof,inserting the modified polynucleotides of said second polynucleotide library into a construct comprising:a signal sequence,a first marker sequence, wherein said first marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

Suitable host cells contemplated for use in this aspect of the present invention can be selected from the group consisting of bacterial cell lines, yeast cell lines, insect cell lines, and mammalian cell lines.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased stability relative to wild type.

In accordance with still another embodiment of the present invention, there are provided methods to modulate the stability of a membrane-spanning protein, said method comprising:generating a polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to modulate the stability thereof,inserting the modified polynucleotides of said second polynucleotide library into a construct comprising:a signal sequence, anda first marker sequence, wherein said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased stability relative to wild type.

In accordance with yet another embodiment of the present invention, there are provided methods to modulate the stability of a membrane-spanning protein, said methods comprising:inserting each member of a modified polynucleotide library into a construct, wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to modulate the stability thereof, said construct comprising:a signal sequence, anda first marker sequence, wherein said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased stability relative to wild type.

In accordance with a further embodiment of the present invention, there are provided methods to modulate the stability of a membrane-spanning protein, said method comprising:transforming suitable host cells with a construct comprising:a modified polynucleotide,a signal sequence, andone or more marker sequences,wherein:said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions,said modified polynucleotide is obtained by generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the stability thereof, and optionally thereaftergenerating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein randomly modified in a sufficient number of amino acid residues so as to increase the stability thereof, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased stability relative to wild type.

In accordance with yet another embodiment of the present invention, there are provided methods to modulate the stability of a membrane-spanning protein, said methods comprising selecting those cells which survive exposure to selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein, wherein said cells are transformed with:a modified polynucleotide,a signal sequence, andone or more marker sequences,wherein:said marker sequence(s) is/are in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions,said modified polynucleotide is obtained by generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein are randomly modified so as to modulate the stability of said membrane-spanning protein, and thereafter optionallygenerating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein randomly modified in a sufficient number of amino acid residues so as to increase the stability thereof.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased stability relative to wild type.

In certain aspects of the present invention, wherein the property to be modulated is the homogeneity of a membrane-spanning protein, the invention method comprises:generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the homogeneity thereof,optionally generating a second polynucleotide library from said first polynucleotide library, said second polynucleotide library comprising a polynucleotide encoding said membrane-spanning protein modified by DNA shuffling in a sufficient number of amino acid residues so as to increase the homogeneity thereof,inserting the modified polynucleotides of said second polynucleotide library into a construct comprising:a signal sequence,a first marker sequence, wherein said first marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions, (e.g., an antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

Suitable host cells contemplated for use in this aspect of the present invention can be selected from the group consisting of bacterial cell lines, yeast cell lines, insect cell lines, and mammalian cell lines.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning proteins have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased homogeneity relative to wild type.

In accordance with still another embodiment of the present invention, there are provided methods to modulate the homogeneity of a membrane-spanning protein, said method comprising:generating a polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the homogeneity thereof,inserting the modified polynucleotides of said second polynucleotide library into a construct comprising:a signal sequence, anda first marker sequence, wherein said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased homogeneity relative to wild type.

In accordance with yet another embodiment of the present invention, there are provided methods to modulate the homogeneity of a membrane-spanning protein, said methods comprising:inserting each member of a modified polynucleotide library into a construct, wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the homogeneity thereof, said construct comprising:a signal sequence, anda first marker sequence, wherein said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent/condition (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased homogeneity relative to wild type.

In accordance with a further embodiment of the present invention, there are provided methods to modulate the homogeneity of a membrane-spanning protein, said method comprising:transforming suitable host cells with a construct comprising:a modified polynucleotide,a signal sequence, andone or more marker sequences,wherein:said marker sequence(s) is/are in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions,said modified polynucleotide is obtained by generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the homogeneity thereof, and optionally thereaftergenerating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein randomly modified in a sufficient number of amino acid residues so as to increase the homogeneity thereof, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased homogeneity relative to wild type.

In accordance with yet another embodiment of the present invention, there are provided methods to modulate the homogeneity of a membrane-spanning protein, said methods comprising selecting those cells which survive exposure to selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein, wherein said cells are transformed with:a modified polynucleotide,a signal sequence, andone or more marker sequence,wherein:said marker sequence(s) is/are in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions,said modified polynucleotide is obtained by generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the homogeneity thereof, and optionally thereaftergenerating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein randomly modified in a sufficient number of amino acid residues so as to increase the homogeneity thereof.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased homogeneity relative to wild type.

In certain aspects of the present invention, wherein the property to be modulated is the crystallizability of a membrane-spanning protein, the invention method comprises:generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the crystallizability thereof,optionally generating a second polynucleotide library from said first polynucleotide library, said second polynucleotide library comprising a polynucleotide encoding said membrane-spanning protein modified by DNA shuffling in a sufficient number of amino acid residues so as to increase the crystallizability thereof,inserting the modified polynucleotides of said second polynucleotide library into a construct comprising:a signal sequence,a first marker sequence, wherein said first marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions, (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

Suitable host cells contemplated for use in this aspect of the present invention can be selected from the group consisting of bacterial cell lines, yeast cell lines, insect cell lines, and mammalian cell lines.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., higher crystallizability relative to wild type.

In accordance with still another embodiment of the present invention, there are provided methods to modulate the crystallizability of a membrane-spanning protein, said method comprising:generating a polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the crystallizability thereof,inserting the modified polynucleotides of said second polynucleotide library into a construct comprising:a signal sequence, anda first marker sequence, wherein said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., higher crystallizability relative to wild type.

In accordance with yet another embodiment of the present invention, there are provided methods to modulate the crystallizability of a membrane-spanning protein, said methods comprising:inserting each member of a modified polynucleotide library into a construct, wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the crystallizability thereof, said construct comprising:a signal sequence, anda first marker sequence, wherein said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., higher crystallizability relative to wild type.

In accordance with a further embodiment of the present invention, there are provided methods to modulate the crystallizability of a membrane-spanning protein, said method comprising:transforming suitable host cells with a construct comprising:a modified polynucleotide,a signal sequence, andone or more marker sequence(s),wherein:said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions,said modified polynucleotide is obtained by generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the crystallizability thereof, and optionally thereaftergenerating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein randomly modified in a sufficient number of amino acid residues so as to increase the crystallizability thereof, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., higher crystallizability relative to wild type.

In accordance with yet another embodiment of the present invention, there are provided methods to modulate the crystallizability of a membrane-spanning protein, said methods comprising selecting those cells which survive exposure to selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein, wherein said cells are transformed with:a modified polynucleotide,a signal sequence, andone or more marker sequence(s),wherein:said marker sequence(s) is/are in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions,said modified polynucleotide is obtained by generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the crystallizability thereof, and optionally thereaftergenerating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein randomly modified in a sufficient number of amino acid residues so as to increase the crystallizability thereof.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., higher crystallizability relative to wild type.

In certain aspects of the present invention, wherein the property to be modulated is the antigenicity of a membrane-spanning protein, the invention method comprises:generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the antigenicity thereof,generating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein modified by DNA shuffling in a sufficient number of amino acid residues so as to increase the antigenicity thereof,inserting the resultant modified genes into a construct comprising:a signal sequence,a first marker sequence, wherein said first marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,transforming suitable host cells with the resulting constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

Suitable host cells contemplated for use in this aspect of the present invention can be selected from the group consisting of bacterial cell lines, yeast cell lines, insect cell lines, and mammalian cell lines.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased antigenicity relative to wild type.

In accordance with still another embodiment of the present invention, there are provided methods to modulate the antigenicity of a membrane-spanning protein, said method comprising:generating a polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the antigenicity thereof,inserting the modified polynucleotides of said second polynucleotide library into a construct comprising:a signal sequence, anda first marker sequence, wherein said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased antigenicity relative to wild type.

In accordance with yet another embodiment of the present invention, there are provided methods to modulate the antigenicity of a membrane-spanning protein, said methods comprising:inserting each member of a modified polynucleotide library into a construct, wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the antigenicity thereof, said construct comprising:a signal sequence, anda first marker sequence, wherein said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased antigenicity relative to wild type.

In accordance with a further embodiment of the present invention, there are provided methods to modulate the antigenicity of a membrane-spanning protein, said method comprising:transforming suitable host cells with a construct comprising:a modified polynucleotide,a signal sequence, andone or more marker sequence(s),wherein:said marker sequence(s) is/are in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions,said modified polynucleotide is obtained by generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the antigenicity thereof, and optionally thereaftergenerating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein randomly modified in a sufficient number of amino acid residues so as to increase the antigenicity thereof, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased antigenicity relative to wild type.

In accordance with yet another embodiment of the present invention, there are provided methods to modulate the antigenicity of a membrane-spanning protein, said methods comprising selecting those cells which survive exposure to selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein, wherein said cells are transformed with:a modified polynucleotide,a signal sequence, andone or more marker sequence(s),wherein:said marker sequence(s) is/are in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions,said modified polynucleotide is obtained by generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the antigenicity thereof, and optionally thereaftergenerating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein randomly modified in a sufficient number of amino acid residues so as to increase the antigenicity thereof.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased antigenicity relative to wild type.

In certain aspects of the present invention, wherein the property to be modulated is the immunogenicity of a membrane-spanning protein, the invention method comprises:generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the immunogenicity thereof,generating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein modified by DNA shuffling in a sufficient number of amino acid residues so as to increase the immunogenicity thereof,inserting the resultant modified genes into a construct comprising:a signal sequence,a first marker sequence, wherein said first marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,transforming suitable host cells with the resulting constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

Suitable host cells contemplated for use in this aspect of the present invention can be selected from the group consisting of bacterial cell lines, yeast cell lines, insect cell lines, and mammalian cell lines.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased immunogenicity relative to wild type.

In accordance with still another embodiment of the present invention, there are provided methods to modulate the immunogenicity of a membrane-spanning protein, said method comprising:generating a polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the immunogenicity thereof,inserting the modified polynucleotides of said second polynucleotide library into a construct comprising:a signal sequence, anda first marker sequence, wherein said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased immunogenicity relative to wild type.

In accordance with yet another embodiment of the present invention, there are provided methods to modulate the immunogenicity of a membrane-spanning protein, said methods comprising:inserting each member of a modified polynucleotide library into a construct, wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the immunogenicity thereof, said construct comprising:a signal sequence, anda first marker sequence, wherein said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased immunogenicity relative to wild type.

In accordance with a further embodiment of the present invention, there are provided methods to modulate the immunogenicity of a membrane-spanning protein, said method comprising:transforming suitable host cells with a construct comprising:a modified polynucleotide,a signal sequence, andone or more marker sequence(s),wherein:said marker sequence(s) is/are in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions,said modified polynucleotide is obtained by generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the immunogenicity thereof, and optionally thereaftergenerating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein randomly modified in a sufficient number of amino acid residues so as to increase the immunogenicity thereof, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased immunogenicity relative to wild type.

In accordance with yet another embodiment of the present invention, there are provided methods to modulate the immunogenicity of a membrane-spanning protein, said methods comprising selecting those cells which survive exposure to selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein, wherein said cells are transformed with:a modified polynucleotide,a signal sequence, andone or more marker sequence(s),wherein:said marker sequence(s) is/are in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions,said modified polynucleotide is obtained by generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to increase the immunogenicity thereof, and optionally thereaftergenerating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein randomly modified in a sufficient number of amino acid residues so as to increase the immunogenicity thereof.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., increased immunogenicity relative to wild type.

In certain aspects of the present invention, wherein the property to be modulated is the conformational selectivity and/or activation pathway selectivity of a membrane-spanning protein, the invention method comprises:generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to modulate the conformational selectivity and/or activation pathway selectivity thereof,generating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein modified by DNA shuffling in a sufficient number of amino acid residues so as to modulate the conformational selectivity and/or activation pathway selectivity thereof,inserting the resultant modified genes into a construct comprising:a signal sequence,a first marker sequence, wherein said first marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,transforming suitable host cells with the resulting constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

As used herein, “conformational selectivity” refers to the activity profile of a polypeptide as a function of the conformation thereof. For example, certain conformations of a peptide or protein may be more or less susceptible to binding or activating other proteins, peptides or compounds. In certain embodiments and aspects, the conformational selectivity embraces samples for which the conformation thereof is substantially homogeneous.

As used herein, “activation pathway selectivity” refers to the ability of an agent to selectively activate one or a limited number of metabolic or signaling pathways from among a plurality of available metabolic or signaling pathways.

Suitable host cells contemplated for use in this aspect of the present invention can be selected from the group consisting of bacterial cell lines, yeast cell lines, insect cell lines, and mammalian cell lines.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., modulated conformational selectivity and/or activation pathway selectivity relative to wild type.

In accordance with still another embodiment of the present invention, there are provided methods to modulate the conformational selectivity and/or activation pathway selectivity of a membrane-spanning protein, said method comprising:generating a polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to modulate the conformational selectivity and/or activation pathway selectivity thereof,inserting the modified polynucleotides of said second polynucleotide library into a construct comprising:a signal sequence, anda first marker sequence, wherein said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., modulated conformational selectivity and/or activation pathway selectivity relative to wild type.

In accordance with yet another embodiment of the present invention, there are provided methods to modulate the conformational selectivity and/or activation pathway selectivity of a membrane-spanning protein, said methods comprising:inserting each member of a modified polynucleotide library into a construct, wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to modulate the conformational selectivity and/or activation pathway selectivity thereof, said construct comprising:a signal sequence, anda first marker sequence, wherein said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions, andoptionally a second marker sequence, wherein said second marker sequence, when present, is downstream of said signal sequence, but upstream of said modified polynucleotide,thereby producing modified polynucleotide-containing constructs,transforming suitable host cells with the modified polynucleotide-containing constructs, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., modulated conformational selectivity and/or activation pathway selectivity relative to wild type.

In accordance with a further embodiment of the present invention, there are provided methods to modulate the conformational selectivity and/or activation pathway selectivity of a membrane-spanning protein, said method comprising:transforming suitable host cells with a construct comprising:a modified polynucleotide,a signal sequence, andone or more marker sequence(s),wherein:said marker sequence(s) is/are in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions,said modified polynucleotide is obtained by generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to modulate the conformational selectivity and/or activation pathway selectivity thereof, and optionally thereaftergenerating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein randomly modified in a sufficient number of amino acid residues so as to modulate the conformational selectivity and/or activation pathway selectivity thereof, andselecting those cells which survive exposure to said selective pressure agent(s)/conditions (e.g., antibiotic) and identifying the modified polynucleotide-containing construct(s) contained therein.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., modulated conformational selectivity and/or activation pathway selectivity relative to wild type.

In accordance with yet another embodiment of the present invention, there are provided methods to modulate the conformational selectivity and/or activation pathway selectivity of a membrane-spanning protein, said methods comprising selecting those cells which survive exposure to selective pressure agent(s) (e.g., antibiotic) and/or selective pressure conditions, and thereafter identifying the modified polynucleotide-containing construct(s) contained therein, wherein said cells are transformed with:a modified polynucleotide,a signal sequence, andone or more marker sequence(s),wherein:said marker sequence is in-frame with said modified polynucleotide, and encodes one or more genes that overcome the sensitivity of said host to the presence of selective pressure agent(s)/conditions,said modified polynucleotide is obtained by generating a first polynucleotide library wherein a sufficient number of bases of a polynucleotide encoding said membrane-spanning protein and/or a sufficient number of amino acid residues of said membrane-spanning protein are randomly modified so as to modulate the conformational selectivity and/or activation pathway selectivity thereof, and optionally thereaftergenerating a second library from said first library, said second library comprising a polynucleotide encoding said membrane-spanning protein randomly modified in a sufficient number of amino acid residues so as to modulate the conformational selectivity and/or activation pathway selectivity thereof.

In accordance with yet another embodiment of the present invention, there are provided modified membrane-spanning proteins identified by the above-described methods. Typically, said modified membrane-spanning protein have one or more improved functional properties relative to the wild type membrane-spanning protein, e.g., modulated conformational selectivity and/or activation pathway selectivity relative to wild type.

In accordance with still another embodiment of the present invention, there are provided methods of generating diagnostic and/or therapeutic antibodies, said methods comprising generating an antibody against any of the modified membrane-spanning proteins described herein. Antibody generation can be carried out using techniques that are well known in the art. Antibody selection can be greatly improved by using thermostabilized mutants prepared employing invention methods. See, for example, Hutchings C J et al. 2014 Mabs, 6(1):246-61).

In accordance with yet another embodiment of the present invention, there are provided methods of treating a disease or condition mediated by a membrane-spanning protein, said method comprising administering an effective amount of a therapeutic antibody prepared as described herein to a subject in need thereof.

In accordance with yet another embodiment of the present invention, there are provided methods of treating a disease or condition mediated by a membrane-spanning protein, said method comprising administering an effective amount of a modified membrane-spanning protein as described herein to a subject in need thereof.

In accordance with yet another embodiment of the present invention, there are provided methods of treating a disease or condition mediated by a membrane-spanning protein, said method comprising administering polynucleotide encoding a membrane-spanning protein according to any of the modified membrane-spanning proteins described herein.

The following examples are provided to further illustrate aspects of the invention. These examples are non-limiting and should not be construed as limiting any aspect of the invention.

Library Generation Phase

Random mutagenesis, optionally coupled with DNA shuffling, can leverage a full range of combinatorial amino acid replacements in multiple positions simultaneously.

Thus, error prone PCR is used to generate a library (first level library) of genes where 8 amino acid residues are randomly mutated after translation per each 1 Kb of DNA (fairly homogeneous frequency of mutation). Optionally, DNA shuffling (StEP, Staggered Extention PCR) may subsequently be used to generate a second library (second level) with genes randomly mutated in about 3 to 15 different amino acids per gene. Roughly ing of this library will contain 1 billion genes; given the amplification method used, about 0.15 billion genes are contained in each ng of DNA used for transformation. Depending on transformation efficiency and effort, about 1 million different genes could be screened each day.

Construct Design

The mutated genes are inserted in a pre-formed construct (plasmid form) containing:

a signal sequence,

maltose binding protein (MBP) or beta-lactamase on the receptor's N-terminal side, and

a kanamycin or gentamicin resistance gene on its C-terminal side.

These domains are all separated by various small oligopeptide linkers. Two exemplary constructs prepared as described herein are illustrated inFIG. 2.

The construct is permanently transcribed inE. coliusing constitutive promoters (e.g. Plac) or inducible promoters (e.g. araBAD, T7) with the addition of chemical inducers (e.g. arabinose, IPTG).

Selection Phase

The construct library containing modified genes is then used to transformE. colistrains (e.g. BL21 or DH10beta). Growth is tested on LB medium with varying concentrations of kanamycin (MIC for untransformed cells is approximately 10 mg/L), both in liquid and agar plates.

An N-terminal truncation (aa 43-424) of the wild type Neurotensin receptor 1 from rat (NTSR1, UniProt P20789) is used as a control system.

About 25 constructs were generated with wild type NTSR1 consisting of combinations of:

several oligopeptide linkers, and

Results

Libraries of mutated GPR55 genes were created containing between about 3 and 15 random mutations (residue) per gene. Selection of plasmids containing enhanced receptor mutants was performed at 50 mg/L kanamycin.

Transformation ofE. colistrains with these libraries resulted in the isolation of mutated GPR55 clones that could confer resistance to high concentrations of kanamycin (>50 mg/L) or carbenicillin (>80 mg/L).

Mutated clones are transferred to mammalian expression hosts, including HEK293T by transfection using the pcDNA3.1 vector. Expression is observed to increase by >5-fold over constructs containing wild type GPR55, as judged by electrophoretic (see, for example,FIG. 3) and fSEC techniques.

Mutated clones are expressed and purified; the resulting protein-detergent complex samples demonstrate an increase in thermostability of up to 7° C. over wild type after one round of mutagenesis (as judged by fSEC (see, for example,FIG. 4) and fluorimetric techniques).

Mutated clones also demonstrate a more homogeneous oligomeric state compared to wild type when receptor samples are purified in ligand free form (see, for example,FIG. 5).

When using hyperthermophilic species (e.g.Thermus thermophilus) as a selection host, the protein melting temperature (in the thermal unfolding format) of the identified mutants is expected to be superior when compared to a selection process performed inE. coli. (i.e., more than 10° C. higher than wild type).

Antibody selection is expected to greatly improve (become easier) when using said thermostabilized mutants as selecting antigen in phage or yeast display (or similar antibody screening, as well as antibody generation). Generating antibody should also be facilitated when these thermostabilized mutants are used as immunogen in-vivo in protein form, or using sequence encoding DNA or RNA in an appropriate animal or human host, or in association with an appropriate immunization vehicle or appropriate immune formulation used as an adjuvant or delivery vehicle (see, for example, Hutchings C J et al. 2014 Mabs, 6(1):246-61).