An inbred corn plant containing dominant genetic information that expresses high protein and increased lysine levels in corn kernels produced by the plant is provided. The genetic information in the plant can be used to produce novel inbred corn lines and hybrid corn lines containing high protein and lysine levels both by established plant breeding techniques and other techniques of genetic manipulation.

INTRODUCTION 
1. Technical Field 
This invention is related generally to the production of maize, commonly 
known in the United States as corn, and more particularly to maize 
containing genetic material capable of causing higher than normal 
production of protein and lysine, and to the unique genetic material 
itself. 
2. Background 
Single-cross hybrid corn is produced by crossing two homozygous inbred 
lines. Homozygosity in an inbred line is achieved by repeated inbreeding; 
in general, by the seventh or eighth generation the inbred line is 
considered genetically pure. Plant breeders basically have two sources of 
germplasm from which to develop new inbreds. They include adapted corn 
belt germplasm and exotic germplasm. The steady increase in corn yield in 
the United States over the past fifty years has been accomplished almost 
exclusively with adapted germplasm. 
Industry's needs for corn are becoming increasingly segmented, opening new 
markets for corn that has specialized characteristics. The grain 
processing industry, especially the dry millers, are interested in new 
hybrids with grain that has an increased amount of hard endosperm and 
which can still be grown competititively against current commercial 
hybrids. The livestock industry, already the largest user of corn, has a 
potential for value-added, nutritionally improved corn. Globally, about 
two thirds of total maize production is used for livestock feed (Glover, 
D. V. and Mertz, E. T. (1987) Agronomy Monograph, 28: 183-336). Maize is 
thus an important food material which supplies 19 percent of the world's 
food calories. Corn also contributes 42 million tons of protein a year, 
which represents 15 percent of the world's annual production of food-crop 
protein. Understandably, high grain yields are the objectives of most corn 
improvement programs, and breeding for nutritional quality or special 
purposes has been of minor importance because of the lack of incentives, 
the lack of communication with the end-user for specific demands, and the 
lack of breeding efforts in using exotic germplasm which can offer some 
unique traits. 
Accordingly, there remains a need for developing unique inbred lines of 
maize that offer genetic diversity and special traits such as increased 
amounts of hard endosperm in the grain and increased nutritive values such 
as high lysine. Additionally, there remains the need to develop the 
genetic material that can control the lysine content in a dominant and 
more stable manner than the previously known recessive opaque-2 (O2) 
system, the best known genetic source of high-lysine corn. 
SUMMARY OF THE INVENTION 
It is the object of the present invention to provide an inbred corn line 
that is significantly different genetically from commercially available 
adapted inbred corn lines. 
It is also an object of this invention to demonstrate that the use of this 
inbred increases the protein and lysine content of resulting single-cross 
hybrids. 
It is still a further object of this invention to demonstrate that the 
nutritive value of a resulting hybrid using this inbred is increased as 
measured by a significant increased rate of gain in livestock. 
These and other objects of the invention have been accomplished by 
providing an inbred corn line (identified as WIL500), hybrid corn lines 
that have the WIL500 line as a parent, and other plant forms, such as cell 
lines and other inbred corn lines, that contain the genetic information of 
the WIL500 line that relates to high lysine and protein production. 
Characteristics of the genetic information are discussed in more detail 
below. 
Seeds of the WIL500 inbred corn line plant have been deposited with the 
Plant Variety Protection Office, NAL Building, Room 500, 10301 Baltimore 
Boulevard, Beltsville, Md. 20705-2351 and have received CORN, FIELD, 
Application No. 8900156. Seeds of the WLL500 inbrid corn line plant have 
been deposited with the American Type Culture Collection (ATCC), 12301 
Parklawn Drive, Rockville, Md. 20852 as ATCC No. 40844.

DETAILED DESCRIPTION OF THE INVENTION 
WIL500 was derived as a self out in an exotic corn breeding experiment 
carried out at Wilson Hybrids, Ind., of Harlan, Iowa. This breeding 
program used corns primarily from tropical origins, especially subtropical 
white dent germplasm, to screen for useful genetic material capable of 
improving inbred corn lines used in producing hybrids for the North 
American corn belt and for other purposes. 
A pedigree breeding method was used for the development of WIL500. In each 
of the six selfing generations during development, the line that 
eventually became WIL500 was selected for on a line-per-se basis for 
agronomic characteristics and specific traits that are discussed below. 
During the last four selfing generations, the line that became WIL500 was 
also evaluated in hybrid combination with other inbreds. In hybrid 
combination, the line was evaluated in replicated yield trials at 
different locations for grain yield, protein quantity, protein quality, 
hybrid agronomic traits, and stability of traits. 
The initial multiplication of WIL500 was made by shelling all the seed from 
a single ear of corn selected by the selfing process as described above 
and planting these seeds in a nursery block. All of the plants that 
resulted were selfed by hand pollination, and at harvest the seeds from 
these ears were bulked and called WIL500. Using this initial seed bulk, 
subsequent seed multiplication was made in an isolation increase field. No 
variants were observed during the seed increase of WIL500. The inbred 
plants appeared stable and uniform in the seed increase fields. 
WIL500 was compared to a public released corn inbred line called AR266 that 
is typical of U.S. corn belt inbred lines to demonstrate the particular 
characteristics of WIL500. Twenty to twenty-five measurements were made 
per trait on each of these inbreds. From these measurements, means, 
variances and t values were calculated. The t values were calculated by 
the following formula: 
##EQU1## 
where: x.sub.1, x.sub.2 =mean 1 and mean 2 respectively; s.sup.2.sub.1, 
s.sup.2.sub.2 =variance 1 and variance 2 respectively; and n=number of 
measurements per mean. 
Statistical differences between the means were determined by the t values 
for n-1 degrees of freedom at the 5% level of probability. Statistically 
significant differences between the means of traits of WIL500 and AR266 
are marked as significant in Table 1. 
TABLE 1 
__________________________________________________________________________ 
Comparison of WIL500 to AR266 
Mean Variance 
Obser./ 
WIL AR WIL AR Calc 
Character 
inbred 
500 266 500 266 t value 
__________________________________________________________________________ 
Tassel 25 9.00 
11.88 
8.42 8.28 3.524* 
branches/ 
tassel 
Tassel 25 16.80 
34.60 
81.00 
133.17 
6.081* 
Branch Angle 
Peduncle 
25 2.88 
3.24 
1.86 1.27 1.017 
Length cm 
No. of 25 14.00 
13.32 
1.42 1.14 2.125* 
leaves/plant 
Leaf Angle 
25 26.60 
29.00 
84.82 
127.08 
0.824 
Ear Leaf 
25 76.28 
72.88 
36.88 
24.28 
2.174* 
Length cm 
Ear Leaf 
25 8.60 
8.84 
0.33 0.47 1.342 
Width cm 
Husk Leaf 
25 1.24 
0.60 
0.19 0.58 3.647* 
Length cm 
Plant 25 129.88 
143.92 
66.36 
636.33 
2.648* 
Height cm 
Ear 25 37.84 
48.00 
27.81 
106.67 
4.381* 
Height cm 
Top Ear 25 5.84 
19.32 
11.72 
25.14 
11.102* 
Internode 
Length cm 
Husk Ex- 
25 7.56 
7.72 
4.09 0.96 0.356 
tension cm 
Shank 25 10.20 
13.40 
1.75 7.50 5.261* 
Length cm 
Shank Inter- 
25 9.76 
10.80 
1.36 1.17 3.269* 
nodes 
Ear 20 14.05 
12.15 
1.94 1.50 4.581* 
Length cm 
Ear Weight gm 
20 30.20 
59.50 
145.75 
389.74 
5.662* 
Kernel Rows/ 
20 13.30 
13.20 
2.22 2.27 0.211 
Ear 
Ear Diameter 
20 35.75 
35.60 
9.46 4.36 0.180 
mm 
Cob Diameter 
20 27.50 
21.95 
7.84 1.31 8.205* 
mm 
100 Kernel 
20 23.58 
25.28 
12.37 
9.25 1.635 
Weight gm 
% Round 20 78.75 
56.60 
315.25 
387.62 
3.736* 
Kernels 
Kernel 20 4.35 
4.90 
1.61 1.57 1.379 
Thickness mm 
Kernel 20 7.60 
7.30 
0.88 0.43 1.172 
Width mm 
Kernel 20 6.90 
8.20 
0.52 0.80 5.060* 
Length mm 
No. of 25 0 0 0 0 0 
Tillers/Plant 
__________________________________________________________________________ 
*Significant 
Additionally, the following differences, although not tested for 
statistical significance, where observed between WIL500 and AR266. as 
compared to AR266, WIL500 flowers two days later, forms kernel black layer 
five days later, reaches 25% kernel moisture two days later, has less 
pollen shed, has a lighter green leaf color, has fewer marginal leaf waves 
amd ore longitudinal creases. WIL500 has a white cob, wherereas AR266 has 
a red cob. 
A number of the characteristics of WIL500 and its hybrids are of particular 
advantage because of requirements of the milling industry. Currently, some 
dry millers pay a premium for corn that meets certain standards in kernel 
characteristics. Acceptable corn must have the following characteristics: 
1. A high percent of bright-yellow, hard-endosperm starch 
2. A high percent of corn oil 
3. Uniform, large-size kernels 
4. An attractive, bright yellow color 
Acceptable corn hybrids on the market today have a range of 56 to 58.8% 
hard endosperm (Table 2). Dry millers have expressed interest in seeing 
future hybrids developed that would have kernels that attained 62% hard 
endosperm and have yields within 10% of leading commercial hybrids. 
TABLE 2 
______________________________________ 
Comparative Milling Characteristic 
of Previous Wilson Corn Hybrids 
Description 
1500b 1700 1700b 1800b 2100 2300 
______________________________________ 
Test Wt. 58.5 59.0 59.3 58.3 59.7 59.3 
lbs/bu 
% Small 3.4 5.6 7.7 5.8 2.4 4.1 
Kernels 
% Hard 57.0 57.3 58.8 56.0 58.8 57.7 
Endosperm 
% Floury 13.8 14.6 13.8 14.7 14.3 14.2 
Endosperm 
% Germ 14.9 14.9 13.5 14.7 13.8 13.9 
______________________________________ 
A selective screening process was initiated on the testing phase of 1500 
hybrids in the breeding program. The criteria consisted of the following: 
1. Protein values to exceed 10%. 
2. Test weight to exceed 59 lbs./bu. (normal=56 lbs./bu.) 
3. Yield comparable to those of standard corn lines. 
4. Plants tolerant to anthracnose. 
5. Plants tolerant to stalk rot, especially fusarium. 
6. Kernels to have a high percent of hard endosperm. 
A sample of a hybrid having WIL500 as a parent, designated as T2021, was 
taken to a dry miller for analysis. The results (Table 3) indicated that 
this grain had a significant increase in the amount of hard endosperm. In 
addition, yield of the T2021 was comparable to leading Wilson hybrids. 
TABLE 3 
______________________________________ 
Comparative Milling Characteristics 
of Wilson Corn Hybrids 
Description 1700 1700b 2100 2300 T2021 
______________________________________ 
Test Wt. lbs/bu 
59.0 59.3 59.7 59.3 63.0 
% Small Kernels 
5.6 7.7 2.4 4.1 0 
% Hard Endosperm 
57.3 58.8 58.8 57.7 64.2 
% Floury Endosperm 
14.6 13.8 14.3 14.2 14.0 
% Germ 14.9 13.5 13.8 13.9 10.0 
______________________________________ 
Protein and Lysine Evaluation 
During the selective screening process discussed above, it was discovered 
that T2021 not only had high levels of hard endosperm, it had 
significantly increased protein and lysine levels compared to regular corn 
(Table 4). All grain samples were obtained from a replicated yield trial, 
which means that the traits of higher protein and lysine are dominant 
since this corn had not been isolated during growth from other sources. 
Isolation of growing corn from other corn fields is required if a 
recessive trait is to be expressed since otherwise cross pollination from 
the other corn will mask the effects of the recessive trait. 
TABLE 4 
______________________________________ 
Composition of Wilson T2021 vs. Regular Corn.sup.1 
Characteristic Regular Corn Wilson T2021 
______________________________________ 
Crude Protein 7.0 to 9.0 10.2 to 10.5 
Lysine (%) 0.23 0.38 (kernel) 
Oil (%) 3.3 to 4.0 4.0 
% Hard Endosperm 
47 to 58 64.2 
% Floury Endosperm 
14 to 15 14 
% Germ 14 to 19 10 
Test Wt. lbs/bu 55 to 60 63 
______________________________________ 
.sup.1 Range of values for commercial yellow dent corn were obtained from 
grain analysis completed by A and L Laboratories and by Lincoln Grain on 
hybrids that are representative of the majority of hybrids used in the 
midwest. Values are based on 12% moisture. The protein, lysine, and oil 
values are very representative of published values which are discussed in 
"Corn: Chemistry and Technology," S. A. Watson and P. E. Ramstad, 1987, 
American Association of Cereal Chemists, Inc., St. Paul, Minnesota. 
In addition to T2021, several other genetic combinations were made with the 
inbred line WIL500 and tested for grain protein levels in several 
experiments. Three WIL500 combinations were compared against a regular 
commercial corn for protein levels under different plant populations and 
different fertilizer rates. In this trial, all combinations of WIL500 
crossed by any adapted foundation inbred line had significantly higher 
grain crude protein levels than commercial corn (adapted foundation 
line.times.adapted foundation line) under all population and fertilizer 
rates tested (Table 5). 
TABLE 5 
______________________________________ 
Grain Protein Analysis of Different 
Combinations of WIL500 Compared 
to Wilson 1700 
Pro- 
duct Pedi- Fert..sup.3 
% Yield 0% H.sub.2 O 
I.D. gree.sup.1 
Pop..sup.2 
Rate H.sub.2 O 
Bu/A Prot. 
Oil 
______________________________________ 
T3138 WIL500 17,000 1x 15.1 158.95 
12.5 5.1 
.times. 23,000 1x 16.0 179.77 
12.3 4.6 
F. Line 17,000 1x + 80 
16.4 152.24 
12.7 4.9 
A 23,000 1x + 80 
16.8 158.45 
12.7 4.9 
T3166 WIL500 17,000 1x 21.0 136.70 
13.3 4.2 
.times. 23,000 1x 20.4 164.56 
12.8 4.6 
F. Line 17,000 1x + 80 
21.2 159.00 
13.7 4.5 
B 23,000 1x + 80 
20.7 161.96 
13.1 4.6 
T3127 WIL500 17,000 1x 16.6 152.32 
12.4 4.5 
.times. 23,000 1x 16.9 153.90 
12.3 5.2 
F. Line 17,000 1x + 80 
15.7 138.18 
12.9 4.5 
C 23,000 1x + 80 
15.5 145.91 
12.4 4.8 
Wilson 
F. Lines 17,000 1x 15.2 162.52 
10.4 4.1 
1700 G .times. H 
23,000 1x 14.9 178.68 
9.6 4.2 
17,000 1x + 80 
14.1 166.85 
10.9 4.3 
23,000 1x + 80 
14.8 170.22 
10.1 4.1 
______________________________________ 
L.S.D. = 0.34 (0.05) 
.sup.1 F. Line = Foundation inbred line 
.sup.2 Pop. = Plant population rate per acre 
.sup.3 Fert. Rate = Fertilizer rate; 
1x = standard rate of 160 pounds of actual nitrogen; 
1x + 80 = 160 pounds nitrogen plus 80 pounds of nitrogen 
An additional trial was conducted in three different Iowa research 
locations to compare the crude protein content of T2021 to the standard 
commercial corn Wilson 1700 across a range of environmental conditions. In 
all locations, T2021 had significantly more grain protein than Wilson 1700 
(Table 6). 
TABLE 6 
______________________________________ 
Grain Protein Analysis of T2021 
(Wilson .times. F Line D) Compared to 
Wilson 1700 Across Three Research 
Locations in Iowa 
Harlan Clarinda N. Sharon 
Mean* 
0% H.sub.2 O 
0% H.sub.2 O 
0% H.sub.2 O 
0% H.sub.2 O 
Pedigree Pro (%) Pro (%) Pro (%) Pro (%) 
______________________________________ 
T2021 WIL500 .times. 
11.2 11.2 11.7 11.4 
F Line D 
1700 LH119 .times. 
9.7 9.7 10.3 9.9 
LH51 
______________________________________ 
*Mean of 7 replications 
Seed of the hybrid T3166 was collected and analyzed (in comparison to 
generic corn) for nutrient composition. The results definitely indicated a 
significant increase in protein and lysine levels (Table 7). 
TABLE 7 
______________________________________ 
Nutrient Composition of Grains Involved in the 
High Protein Corn Evaluation Study.sup.1 
Exper. high 
Generic protein corn 
Component Yellow Corn.sup.2 
(T3166) 
______________________________________ 
Metabolizable 
energy 
KCal/lb 1530 1530 
KCal/kg 3370 3370 
Fat % 3.8 3.8 
Linoleic acid % 
1.9 1.9 
Crude Protein % 
8.9 11.2 
Lysine % .24 .32 
______________________________________ 
.sup.1 Nutrient composition values are based on 12% moisture 
.sup.2 Generic corn is from the 1987 crop of commercial yellow dent corn 
representative of corn used in Nebraska for feeding livestock 
the grain of the inbred line WIL500 was also tested for crude protein and 
lysine levels and compared to other inbred lines (Table 8). The results 
clearly indicated that the lysine level of WIL500 was similar to that of 
inbred lines that possessed the recessive opaque-2 gene, and far superior 
to standard foundation inbred lines. The previous analysis (Table 7) 
clearly indicated that hybrids with WIL500 did have a significantly higher 
lysine content. An additional important fact is that the increase in 
protein and lysine in the hybrid was obtained without isolating the field. 
This is a significant advantage over use of the opaque-2 system, as the 
recessive opaque-2 system (O2) requires field isolation. 
TABLE 8 
______________________________________ 
Protein and Lysine Analysis of the Grain of 
Various Inbreds and of Hybrid Combinations 
Product I.D. Protein (%) 
Lysine (%) 
______________________________________ 
WIL500 (Harlan Nursery) 
10.38 0.44 
Tuxpeno QPM 02 10.06 0.46 
IPTT42 QPM 02 9.76 0.39 
Mo17 02 11.18 0.47 
Foundation Line A 9.08 0.31 
Foundation Line B 9.03 0.29 
Foundation Line C 9.28 0.31 
______________________________________ 
To determine if the increased protein levels of the experimental corn T3166 
(WIL500.times.adapted inbred B) also increase nutritive value, two feeding 
trials were conducted. The first used grains in diets considered low in 
protein for starting chicks (18.4% protein). The rationale for this was 
that if the experimental corn was truly higher in protein, chicks would 
gain more weight. In trial two, recommended (NRX, 1984) protein levels 
(22% in the starter, 19% in the grower) were used, and the birds were fed 
to 42 days of age. In each trial, body weight gain and feed efficiency 
were used as response criteria. 
Results of feeding trials one and two are shown in Table 9. In the first 
trial, chicks receiving the experimental high protein corn diet gained 
about 40 grams (about 0.10 pound) more weight than chicks fed diets with 
the other grain sources. Therefore, this cultivar of corn can be called 
high protein since the protein level is great enough to end the need for 
soybean meal, and chicks still grow at a fast rate. In trial two, there 
were no significant differences in 21-day weight gains (these diets 
contained recommend instead of low protein levels). However, at 42 days 
the birds fed diets containing high protein corn had gained 117 grams and 
70 grams more (about 0.25 and 0.15 pound) than birds fed generic corn and 
grain sorghum diets, respectively. 
TABLE 9 
______________________________________ 
Performance of Chicks Fed High Protein Corn, 
Generic Corn, and Grain Sorghum 
Trial 1 Trial 2 
Body wt Feed/ Body wt Feed/ 
gain gain gain gain Shank 
(grama).sup.1 
ratio (grama).sup.2 
ratio color 
Treat- Day Day Day Day Day Day Day 
ment 21 21 21 42 21 42 42 
______________________________________ 
Generic 
585a 1.68b 571a 1633a 1.64b 
2.00b 
7.3c 
Corn 
Experi- 
627b 1.57a 590a 1750b 1.57a 
1.96a 
6.5b 
mental 
High 
Protein 
Corn 
(#3186) 
Sorghum 
588a 1.61ab 596a 1680a 1.54a 
1.94a 
1.0a 
(NC + 
271) 
______________________________________ 
.sup.1 Each value is the average of 5 replicate pens containing 6 Vantres 
X Arbor Acre chicks per pen. 
.sup.2 Each value is the average of 5 replicate pens of males and 5 
replicate pens of females containing 6 Vantress X Arbor Acre chicks per 
pen. 
a, b, c Values within the same column which are followed by different 
subscripts are statistically different (P &lt; .05). 
In summary, an experimental high protein corn with increased lysine levels 
improved performance of broiler chicks receiving both low and recommended 
or standard protein levels in their diets. High protein corn has the 
potential to reduce the need for soybean meal in poultry diets. 
Restriction Fragment Length Polymorphism 
Restriction fragment length polymorphism (RFLP) has been extensively used 
in human and plant genetics for varietal and parental lineage 
identification. A complete description of this technology is described in 
Soller, M. and J. S. Bockmann, Genetic polymorphism in varietal 
identification and genetic improvment, (1988) Theor. Appl. Genet. 67: 
25-33 and Heleutjaris, T., A genetic linkage map of maize based on RFLPs 
(1987) TIG 3: 217-221. In this technology DNA is isolated from the 
seedlings obtained after planting seed of the corn line to be tested, 
using published techniques. After restriction with HIND III, a 
commercially available restriction enzyme, the DNA is tested with DNA 
probes. In the case reported here UMC probes and BNL probes were obtained 
from Dr. D. Hoisington, 303 Curtis Hall, University of Mo., Columbia, Mo. 
65211 and Dr. F. A. Burr, Biology Department, Brookhaven National 
Laboratory, Upton, N.Y. 11973. In addition to WIL500 line, DNA was also 
extracted from MO17, B73 and LH123 corn homozygous lines. The size of the 
DNA fragments reacting with the probes (Table 10) indicate a specific 
pattern of the WIL500 line which identify the genetic material of this 
corn line. 
TABLE 10 
______________________________________ 
Characterization of the Line WIL500 
Length of Restriction Fragment 
in Kb 
Probe WIL500 LH123 MO17 B73 
______________________________________ 
UMC83 10 3.9 9.8 2.7 
BNL12.06 4.1 4.1 18.4 3.7 
UMC5 2.7 4.2 2.6 2.6 
UMC34 7.5 18.0 4.8 5.6 
UMC131 3.8 3.8 -- -- 
UMC139 5.3 5.3 6.4 4.5 
BNL8.45 11.0 11.0 8.6 8.6 
UMC10 5.3 7.3 7.2 5.2 
UMC102 6.7 6.7 5.7 5.5 
UMC19 3.9 2.3 -- -- 
UMC56 6.2 6.2 2.3 2.3 
UMC151 2.3 4.4 2.7 2.7 
BNL14.07 10.0 10.0 -- -- 
BNL15.21 7.0 20.0 -- -- 
______________________________________ 
Characteristics of WIL500 are as follows: 
Plant color--dark green 
Emergence--Average to good (similar to B73) 
Vigor--Average to good (similar to B73) 
Stalk 
Height (cm)--129.88 
Ear Height (cm)--37.84 
Anthycyanin--Stalks are green with no visual evidence of anthocyanin 
pigment 
Tillers/Plant--0 
Brace roots--Good brace root development at the base. Color=green 
Leaves 
Angle--26.60 
Number/Plant--1400 
Leaf Sheath 
Anthocyanin--Leaf sheath is dark green with no visual evidence of 
anthocyanin pigment 
Pubesence--Heavy (similar to OH26) 
Width (cm)--8.60 
Ear leaf length (cm)--76.28 
Tassel 
Length (cm)--23.0 
Branch angle (cm)--16.80 
Branching--9.00 
Anther color--Green 
Peducle length (cm)--2.88 
Ear 
Silk color--Green 
Husk bracts--Buff in color 
Ear/Stalk--one 
Length (cm)--14.05 
Weight (gm)--30.20 
Shape--Conical 
Diameter (mm)--35.75 
Top ear internode length (cm)--5.84 
Kernel Rows/Ear--13.30 
Shank length (cm)--10.20 
Shank internodes--9.76 
Husk number --9-11 
Husk extension (cm)--7.56 
Husk leaf length (cm)--1.24 
Husk width (cm) --5 
Cob color--White 
Cob diameter (cm)--27.50 
Kernel 
Type--Flint 
Color--Yellow 
Cap--Medium yellow 
Sides--Dark yellow 
Thickness (mm)--4.35 
Form--78.75% round kernels 
Width (mm)--7.60 
Length (mm)--6.90 
Weight 100 Kernels (gm)--23.58 
Texture--&gt;58% of a very hard endosperm with very little white floury starch 
present 
Pollen Shed 
Days--75 
Degree Days--1745 
50% Silk 
Days--79 
Degree days--1765 
Shank diameter (cm)--1.5 
Glume Color--Green 
Glume band--Red 
Tassel--branch angle --16.8.degree. 
Brace root color--green 
Days to flower--75-79 
Days to black layer--148 (from emergence) 
Days to 25% kernel moisture--158 (from emergence) 
All publications and patent applications cited in this specification are 
herein incorporated by reference as if each individual publication or 
patent application were specifically and individually indicated to be 
incorporated by reference. 
Although the foregoing invention has been described in some detail by way 
of illustration and example for purposes of clarity of understanding, it 
will be readily apparent to those of ordinary skill in the art in light of 
the teachings of this invention that certain changes and modifications may 
be made thereto without departing from the spirit or scope of the appended 
claims.