1,1,1-Trifluoro-2-hydroxy-5-benzyloxy compounds represented by the following formula (I) and optical isomers thereof: ##STR1## The compounds of the present invention are R-form and S-form when X is --C.tbd.C-- and are trans-form and cis-form both of which have respectively R-form and S-form when X is --CH.dbd.CH-- or ##STR2## ##STR3## the compound of the present invention is ##STR4## The compounds of the present invention are useful for asymmetric introduction of trifluoromethyl group and molecular designing of biologically active substances, ferroelectric liquid crystal compounds and so on.

The present invention relates to optically active 
1,1,1-trifluoro-2-hydroxy-5-benzyloxy compounds and a process for 
asymmetrically introducing the trifluoromethyl group by incorporating the 
hydroxy group at the 2-position to the other molecules. The compounds have 
industrial significance in molecular designing of biologically active 
substances, ferroelectric liquid crystal compounds and so on. 
Fluorine compounds have specificity as compared with hydrocarbons. It is 
considered that this specificity is based on higher electronegativity of 
the fluorine atom than the other atoms, sterically small molecular volume 
next to the hydrogen, and large carbon-fluorine bond energy. 
Fluorine atom has the highest electronegativity among all elements (F: 
4.00, O: 3.50, Cl: 3.15, N: 3.05). This means that electron density of 
carbons at .alpha.-position and .beta.-position of the C-F bond is made 
smaller. For instance, when it has an amino group at the .alpha.-position, 
basicity of the amino group is made extremely small and, when it has an 
hydroxyl group at the .beta.-position, the behavior of the hydroxyl group 
is more acidic. In some cases, the fluorine atom influences conformation 
of the whole molecule. Therefore, when a compound is modified with a 
fluorine atom, especially a trifluoromethyl group, chemical properties and 
reactivity of the compound per se undergo conspicuous change and in some 
cases, organic synthesis methods are hardly used freely. 
Hitherto, introduction of a fluorine atom or a fluorine-containing group 
into a compound has been mostly conducted by direct fluorination method by 
using fluorinating agents. For example, fluorination of alcohols is 
conducted with organic reagents such as HPDA reagent 
(hexafluoropropenedialkylamine adduct) and DAST reagent 
(dialkylaminosulfur trifluoride). Furthermore, halogen exchanging process 
by metal fluorides such as CsF, KF, and AgF or TBAF (tetrabutylammonium 
fluoride) has been well known to be used for tosylate, mesylate and 
halides derived from alcohols. In additinn, there are fluorination by ring 
opening of oxirane, addition reaction of fluorine to double bonds, and 
fluorination of carbonyl compounds. 
As explained above, synthesis of various optically active 
trifluoromethyl-containing compounds has become relatively simple, but for 
molecular designing of biologically active substances based on these 
optically active substances, asymmetric propagation to a plurality of 
carbon atoms in the molecule becomes necessary. In such case, asymmetric 
induction to carbon in the molecule of optically active compounds having a 
trifluoromethyl group is difficult and has limit due to specificity of 
fluorine atoms when modified with fluorine atoms, especially a 
trifluoromethyl group. 
The present invention provides compounds represented by the formula (I) and 
optical isomers thereof, and a process for preparation thereof. 
##STR5## 
The present invention has established a process for obtaining optically 
active 1,1,1-trifluoro-2-hydroxy-5-benzyloxy-3-pentyne, wherein propargyl 
alcohol which is inexpensive and readily available, is used as a starting 
material, 1,1,1-trifluoro-2-hydroxy-5-benzyloxy-3-pentyne is once 
prepared, and the pentyne compound is subjected to optical resolution by 
use of enzyme or lipase until optically active 
1,1,1-trifluoro-2-hydroxy-5-benzyloxy-3-pentyne, optically active 1,1,1- 
trifluoro-2-hydroxy-5-benzyloxy-3-pentene and 
1,1,1-trifluoro-3-(N-phenylamino-5-benzyloxypentane-2,4-diol are obtained. 
Route of chemical reaction according to the present invention will be 
explained below. 
(1) Propargyl alcohol is allowed to react with chloromethylbenzyl ether to 
obtain 1-benzyloxy-2-propyne (1). Then, this is allowed to react with 
Grignard reagent and a trifluoroacetic acid ester to obtain 
5-benzyloxy-1,1,1-trifluoro-3-pentyne-2-one (2). Then, this is allowed to 
react with hydride of boron or aluminum to obtain 
5-benzyloxy-1,1,1-trifluoro-3-pentyne-2-ol (3). This is acetylated or 
isobutylated and thereafter lipase or lipase-producing microorganism is 
allowed to act thereon to obtain optically active 
(R)-5-benzyloxy-1,1,1-trifluoro-3-pentyne-2-ol [(R)-(3)] and optically 
active (S)-5-benzyloxy-1,1,1-trifluoro-2-acetoxy (or butoxy)-3-pentyne 
[(S)-(4)]. 
Furthermore, if necessary, 
(S)-5-benzyloxy-1,1,1-trifluoro-2-hydroxy-3-pentyl [(S)-(4)] is also 
obtained by hydrolysis of acetate (or isobutoxylate) of the above (S)-(4). 
The above process is shown by reaction formula as follows. 
##STR6## 
Next, a hydride of boron or aluminum or palladium catalyst is allowed to 
act on 5-benzyloxy-1,1,1-trifluoro-3-pentyne-2-ol (compound (3)) to obtain 
5-benzyloxy-1,1,1-trifluoro-3-pentene-2-ol (E)-(5) or (Z)-(5). This is 
acetylated or isobutylated and then thereon is allowed lipases or 
lipases-producing microorganism to act to obtain optically active 
(R)-5-benzyloxy-1,1,1-trifluoro-3-pentene-2-ol [(R)-(E)-5] or [(R)-(Z)-5] 
and optically active (S)-2-acetoxy (or 
butoxy)-5-benzyloxy-1,1,1-trifluoro-3-pentene [(S)-(E)-6 or (S)-(Z)-6]. 
Furthermore, if necessary, 5-benzyl-oxy-1,1,1-trifluoro-3-pentene-2-ol 
[(S)-(E)-(5) or (S)-(Z)-(5)] can also be obtained by hydrolysis of 
acetate or isobutoxylate of the above [(S)-(E)-6[ or ](S)-(Z)-6]. 
The above reaction is shown by chemical formula as follows: 
##STR7## 
The following optical isomers are obtained also for (Z)-(5) by asymmetric 
resolution in the same manner as for (E)-(5). 
##STR8## 
Optically active compounds produced by the present process are as follows: 
______________________________________ 
Configuration 
Chemical structure 
______________________________________ 
(S)-(E) 
##STR9## 
(S)-(Z) 
##STR10## 
(R)-(E) 
##STR11## 
(R)-(Z) 
##STR12## 
(S)-(E) 
##STR13## 
(S)-(Z) 
##STR14## 
(R)-(E) 
##STR15## 
(R)-(Z) 
##STR16## 
______________________________________ 
Furthermore, 5-benzyloxy-1,1,1-trifluoro-3-pentene-2-ol [(E)-(5) or 
(Z)-(5)] is allowed to react with a peroxide of an organic acid to obtain 
5-benzyloxy-trans or cis-3,4-epoxy-1,1,1-trifluoro-2-pentanol (7). 
Then, the above epoxide (7) is allowed to react with an isocyanic acid 
ester to obtain 5-benzyloxy-trans (or 
cis)-3,4-epoxy-2-[(N-phenylcarbamoyl)oxy]-1,1,1-trifluoropentane (8). 
Then, the resulting compound (8) is allowed to react with an alkali metal 
alcoholate to obtain a mixture of the above trans epoxide (7) or cis 
epoxide (7') and urethan (9). This mixture is allowed to react with an 
alkali metal hydroxide to obtain the above trans epoxide (7) or cis 
epoxide (7') and 
5-benzyloxy-3-(N-phenylamino)-1,1,1-trifluoropentane-2,4-diol (10). 
The above compounds (7) and (10) are both optically active compounds having 
three continuous asymmetric points. 
The above reaction is shown by chemical formula as follows: 
##STR17## 
is prepared in the same procedure as above from (S)-(E)-(5) (trans) 
##STR18## 
Reaction of the compound (Z)-(5) is also shown by the following chemical 
formulas. 
##STR19## 
is prepared in the same manner as above from (S)-(Z)-(5) (cis) 
##STR20## 
Optically active compounds which are obtained by the present process are 
shown below. 
______________________________________ 
Configuration 
Chemical structure 
______________________________________ 
2S, 3R, 4R 
##STR21## 
2S, 3S, 4S 
##STR22## 
2R, 3R, 4R 
##STR23## 
2R, 3S, 4S 
##STR24## 
2S, 3R, 4R 
##STR25## 
2S, 3S, 4S 
##STR26## 
2S, 3R, 4R 
##STR27## 
2R, 3S, 4S 
##STR28## 
Anti-form 
##STR29## 
Anti-form 
##STR30## 
______________________________________

The present invention will be explained in detail by the following 
examples. 
EXAMPLES 
(1) Preparation of 1-benzyloxy-2-propyne (1) 
4.6 g (192 mmol) of sodium hydride was charged in a 200 ml three-necked 
flask provided with a 100 ml dropping funnel with a bypass and 
vacuum-dried in nitrogen stream. Then, thereto was added 100 ml of 
tetrahydrofuran, followed by cooling to 0.degree. C. Furthermore, 11.2 ml 
(192 mmol) of propargyl alcohol diluted with 100 ml of tetrahydrofuran was 
gradually added thereto. After stirring at this temperature for 30 
minutes, 22.3 ml (160 mmol) of chloromethylbenzyl ether diluted with 20 ml 
of tetrahydrofuran was added dropwise, followed by stirring for 30 
minutes. After further stirring for 1 hour at room temperature, the 
reaction was stopped by saturated aqueous ammonium chloride solution and 
the reaction mixture was rendered weakly acidic with 3N aqueous 
hydrochloric acid solution and extracted with methylene chloride. The 
organic layer was dried over anhydrous magnesium sulfate. The solvent was 
removed by vacuum distillation and then the ether (1) was obtained by 
vacuum distillation. Boiling point: 80-83.degree. C./ 0.5 mmHg, Yield: 90% 
.sup.1 H-NMR.delta.(ppm): 2.28(t.1H, J=3.0 Hz. CH.tbd.C-); 4.28(d.2H, J=3.0 
Hz CH.tbd.C-CH.sub.2); 4.63(s.2H, CH.sub.2 Ph); 4.83(s.2H, O-CH.sub.2 O); 
7.58(s.5H, Ph). 
IR(neat)(cm.sup.-1): 3300(C.tbd.C), 3050, 2900(CH.sub.2), 1500(Ph) 
(2) Preparation of 5-benzyloxy-1,1,1-trifluoro-3-pentyne-2-one (2) 
4.2 g (172 mg-atm) of magnesium was charged in a 200 ml three-necked flask 
equipped with a Dimroth condenser and a 100 ml dropping funnel with a 
bypass and vacuum-dried in argon stream. Thereto were added 20 ml of dry 
tetrahydrofuran and 0.84 ml of ethyl bromide and after initiation of 
reaction, a solution prepared by dissolving 12.00 ml (total amount 12.84 
ml, 172 mmol) of ethyl bromide in 140 ml of tetrahydrofuran was gradually 
added, followed by stirring for 1.5 hour to obtain Grignard reagent. Then 
thereto was added ether (1) (143 mmol) prepared by the method of the above 
(1) diluted with 20 ml of tetrahydrofuran over a period of 30 minutes, 
followed by stirring for 1 hour at 40.degree. C. Then, separately, 28.4 g 
(200 mmol) of ethyl trifluoroacetate and 80 ml of tetrahydrofuran were 
charged in a 300 ml three-necked flask in argon stream and cooled to 
-98.degree. C. Thereto was added the Grignard reagent obtained above over 
a period of 1 hour, followed by stirring at this temperature for 1.5 hour 
and then, for further 1 hour at 0.degree. C. 
Then, 1N aqueous hydrochloric acid solution was added thereto and the 
reaction mixture was made weakly acidic and extracted with methylene 
chloride. The organic layer was dried over magnesium sulfate and solvent 
was distilled off to obtain crude product ketone (2). This compound was 
susceptible to decomposition by distillation and so was used in the next 
step as it was without purification. 
(3) Preparation of 5-benzyloxy-1,1,1-trifluoro-3-pentyne-2-ol (3) 
1.85 g (48.9 mmol) of sodium borohydride and 100 ml of absolute ethanol 
were charged in a 200 ml eggplant type flask and then thereto was added a 
solution prepared by dissolving the crude ketone (the total amount) 
obtained in the above (2) in 60 ml of ethanol over a period of 1 hour 
under ice-cooling, followed by stirring at room temperature overnight. 
Ethanol was removed by vacuum distillation and thereafter, the reaction 
was stopped by adding saturated aqueous solution of ammonium chloride. 
Then, the reaction mixture was made weakly acidic with 1N aqueous 
hydrochloric acid solution and was extracted with methylene chloride. The 
organic layer was dried over anhydrous magnesium sulfate and then the 
solvent was distilled off to obtain a crude product. The resulting crude 
product was purified by silica gel column chromatography to obtain the 
above alcohol (3). 
Yield: 88% 
.sup.1 H-NMR.delta.(ppm): 3.63(bs.1H, OH); 4.30(d.2H, J=1.5 Hz 
CH.vertline.C-CH.sub.2 O-); 4 62[m.1H, CF.sub.3 CH(OH)]; 4.63(s.2H, 
CH.sub.2 Ph); 4.82(s.2H, O-CH.sub.2 O); 7.38(s.5H, Ph). 
.sup.1 9 F-NMR: 1.00(d, JH-F=5.6 Hz) 
IR(neat)(cm.sup.-1): 3400(OH), 3050, 2900(CH.sub.2) 
(4) Preparation of 2-acetoxy-5-benzyloxy-1,1,1-trifluoro-3-pentyne (4) 
In a three-necked flask of 50 ml in internal volume which was well dried 
under reduced pressure in nitrogen stream were charged 20 ml of methylene 
chloride, 3.57 g (13.0 mmol) of the above alcohol (3) and 1.20 ml (16.9 
mmol) of acetyl chloride and then 1.37 ml (16.9 mmol) of pyridine was 
added thereto under ice-cooling, followed by stirring at room temperature 
overnight. The reaction was stopped by adding 1N aqueous hydrochloric acid 
solution and the reaction mixture was extracted with methylene chloride 
and dried over anhydrous magnesium sulfate. Solvent was distilled off to 
obtain a crude product, which was purified by silica gel column 
chromatography to obtain the above acetate (4). 
Yield: 96% 
.sup.1 H-NMR.delta.(ppm): 2.17(s.3H, CH.sub.3 CO); 4.30(d.2H, J=1.5 Hz, 
CH.vertline.C-CH.sub.2); 4.63(s.2H, CH.sub.2 Ph); 4.80(s.2H, O-CH.sub.2 
O); 5.92 [tq.1H, J=1.5, 6.4 Hz, CF.sub.3 CH(OCOCH.sub.3)]; 7.38(s.5H, Ph). 
.sup.1 9 F-NMR: -1.17(d, JH-F=6.4 Hz) 
IR(neat)(cm.sup.-1): 2950, 2900(CH.sub.2), 1770(C=0) 
For the corresponding isobutyrate, 
5-benzyloxy-2-isobutyryloxy-1,1,1-trifluoro-3-pentyne was obtained in the 
same manner as for acetate. 
.sup.1 H-NMR.delta.(ppm): 1.23 [d.6H, J=7.l Hz.)(CH.sub.3).sub.2 CH.]; 2.67 
[sep.1H, J=7.1 Hz)(CH.sub.3).sub.2 CH.]; 4.32(d.2H, J=2.0 Hz) 
CH.vertline.C-CH.sub.2 ; 4.65(s.2H, CH.sub.2 Ph); 4.82(s.2H, O-CH.sub.2 
O); 5.95(tq.1H, J=2.0, 6.0 Hz CF.sub.3 CH); 7.40(s.5H, Ph). .sup.1 9 
F-NMR: -1.58(d, JH-F=6.0 Hz) 
IR(neat)(cm.sup.-1): 3000, 2950(CH.sub.2), 1760(C=0) 
(5) Asymmetric hydrolysis of acetate (4) 
In an eggplant type flask of 200 ml in internal volume were charged 4.3 g 
(13.7 mmol) of the acetate obtained in the above (4), 2.3 g (135470 units) 
of lipase MY and 130 ml of distilled water, followed by stirring at 
40.degree.-41.degree. C. Acetic acid produced in the system with progress 
of reaction was titrated with 1N aqueous sodium hydroxide solution to 
check degree of progress of hydrolysis. When suitable degree of hydrolysis 
was obtained, reaction mixture was filtrated by Celite. Then, filtrate was 
extracted with ethyl acetate and the organic layer was dried over 
anhydrous magnesium sulfate. Thereafter, solvent was distilled off under 
reduced pressure and the resulting crude product was purified by silica 
gel column chromatography where a moving phase was n-hexane/diethyl ether 
(10/1) to obtain 1.50 g (5.50 mmol) of the corresponding optically active 
alcohol (R)-(3) and 1.90 g (6.00 mmol) of optically active acetate (S)-(4) 
(recovery: 84%). 
The same procedure as above was also carried out with using isobutyrate as 
a starting material. The results are shown in Table 1. 
TABLE 1 
______________________________________ 
Degree of 
Optical 
Starting hydrolysis 
purity 
Material (3) 
Enzyme Supplier (%) (% ee) 
______________________________________ 
Acetate Lipase MY Meito Sangyo 
45 30 
Co. 
Acetate Lipase P Amano Seiyaku 
33 49 
"Amano" Co. 
Isobutyrate 
Lipase MY Meito Sangyo 
50 -- 
Co. 
Isobutyrate 
Lipase P Amono Seiyaku 
34 88 
"Amano" Co. 
______________________________________ 
(6) Preparation of 5-benzyloxy-1,1,1-trifluoro-3-pentene-2-ol (E)-(5) 
18 ml (61.2 mmol) of 3.4 N toluene solution of NaAlH.sub.2 (OCH.sub.2 
CH.sub.2 OCH.sub.3).sub.2 (Red-Al.RTM.) and 50 ml of dry diethyl ether 
were charged in a sufficiently dried 100 ml three-necked flask provided 
with a 50 ml dropping funnel with a bypass in nitrogen stream and cooled 
to -20.degree. C. Thereto was added over a period of 30 minutes a solution 
prepared by diluting 14.0 g (51.0 mmol) of 
5-benzyloxy-1,1,1-trifluoro-3-pentyne-2-ol (3) obtained in the above (3) 
with 50 ml of ether. Stirring was carried out at this temperature for 1 
hour and then for further 1 hour at room temperature. The reaction was 
stopped by using 3 N aqueous hydrochloric acid solution and reaction 
mixture was extracted with ether, dried over anhydrous magnesium sulfate 
and concentrated. The resulting crude product was purified by silica gel 
column chromatography to obtain the compound (E)-(5). Yield 83%. 
.sup.1 H-NMR.delta.(ppm): 2.85(d, 1H, J=6.0 Hz, OH); 4.15 (d, 2H, J=4.5 Hz, 
C=CH-CH.sub.2); 4.38 (m, 1H, CF.sub.3 CH); 4.63 (s, 2H, CH.sub.2 Ph); 4.78 
(s, 2H, O-CH.sub.2 O); 5.80 (dd, 1H, J=5.3, 16.5 Hz, CF.sub.3 CHCH=CH); 
6.12 (dt, 1H, J=4.5, 16.5 Hz, CF.sub.3 CHCH=CH); 7.40 (s, 5H, Ph). 
.sup.1 9 F-NMR: +0.75 (d, J.sub.H-F =6.0 Hz) 
IR(neat)(cm.sup.-1): 3400 (OH), 3050, 2900 (CH.sub.2) 980 (C=C trans) 
(7) Preparation of 2-isobutoxy-5-benzyloxy-1,1,1-trifluoro-3-pentene 
(E)-(6) 
To a solution of 11.6 g (42.0 mmol) of the compound (E)-(5) obtained in the 
above (6) and 5.3 ml (50.4 mmol) of isobutyric acid chloride in 50 ml of 
methylene chloride, was added 4.1 ml (50.4 mmol) of pyridine at 0.degree. 
C. and temperature was returned to room temperature and the solution was 
stirred overnight. Then, reaction was terminated by 1N aqueous 
hydrochloric acid solution and the reaction mixture was extracted with 
methylene chloride and dried over anhydrous magnesium sulfate and solvent 
was distilled off under reduced pressure. The resulting crude product was 
purified by silica gel column chromatography to obtain the above 
isobutyrate (E)-(6). Yield: 84%. 
.sup.1 H-NMR.delta.(ppm): 1.20 [d, 6H, J=7.1 Hz, CH(CH.sub.3).sub.2 ]; 2.67 
[sep.1H, J=7.1 Hz, CH(CH.sub.3).sub.2 ]; 4.15(d, 2H, J=4.5 Hz, 
CH=CH-CH.sub.2); 4.63(s, 2H, CH.sub.2 Ph; 4.80(s, 2H, O-CH.sub.2 -O-); 
5.90(m, 2H, CF.sub.3 CH, CF.sub.3 CHCH=CH); 6.27(dt, 1H, J=4.2, 15.0 Hz, 
CF.sub.3 CHCH=CH; 7.40(s, 5H, Ph). 
.sup.1 9 F-NMR: -1.17 (d, J.sub.H-F =6.2 Hz) 
IR(neat)(cm.sup.-1): 3000, 2950(CH.sub.2), 1760(C=0) 
For the corresponding acetate, 
2-acetoxy-5-benzyloxy-1,1,1-trifluoro-3-pentene (E)-(6) was also obtained 
in the same manner as for isobutyrate. Yield: 94%. 
(8) Asymmetric hydrolysis of the above isobutyrate (E)-(6) 
6.24 g (21.6 mmol) of the above isobutyrate (E)-(6), 7.2 g (216000 units) 
of lipase P (manufactured by Amano Seiyaku Co.), and 150 ml of distilled 
water were charged in an eggplant type flask of 50 ml in internal volume 
and stirred with keeping temperature at 40.degree.-51.degree. C. in a 
thermostatic bath. Isobutyric acid was produced in the reaction system 
with progress of reaction and degree of progress was checked with 
titrating the isobutyric acid with 1N aqueous sodium hydroxide solution. 
Reaction mixture was filtrated by Celite and extracted with ethyl acetate 
and dried over anhydrous magnesium sulfate and solvent was distilled off 
under reduced pressure. Crude product was purified by silica gel column 
chromatography where a moving phase was n-hexane/diethyl ether (10/1) to 
obtain 1.11 g (4.00 mmol, optical purity 98% ee) of the corresponding 
alcohol (R)-(E)-(5) and 5.06 g (14.6 mmol) of isobutyrate (R)-(E)-(6). 
Recovery: 86%. 
When acetate (E)-(6) was used as a starting material, the above compound 
(R)-(E)-(5) was also similarly obtained. Recovery: 90%, optical purity 40% 
ee. 
(9) Preparation of 5-benzyloxy-trans-3,4-epoxy-1,1,1-trifluoro-2-pentanol 
(7) 
In an eggplant type flask of 100 ml in internal volume were charged at 
0.degree. C. 4.14 g (15.0 mmol) of 
5-benzyloxy-1,1,1-trifluoro-3-pentene-2-ol (E)-(5), 5.29 g (30.7 mmol) of 
m-chloroperoxybenzoic acid (mCPBA) and 80 ml of methylene chloride, 
followed by stirring at room temperature overnight. Thereto was added 
saturated aqueous sodium sulfite solution to decompose excess mCPBA and 
then reaction mixture was extracted with methylene chloride. The organic 
layer was dried over anhydrous magnesium sulfate and then solvent was 
distilled off under reduced pressure. The resulting crude product was 
purified by silica gel column chromatography. The above trans epoxide (7) 
was obtained in a yield of 90%. 
Diastereomer was a mixture of diastereomers of 55:45 from integral ratio of 
a peak appearing in low magnetic field and a peak appearing in high 
magnetic field in .sup.1 9 F-NMR. 
.sup.1 9 F-NMR: -0.47(d, J.sub.H-F =7.5 Hz); -0.42(d, J.sub.H-F =7.2 Hz) 
(10) Preparation of 
5-benzyloxy-trans-3,4-epoxy-2-[(N-phenylcarbamoyl)oxy]-1,1,1-trifluoropent 
ane (8) 
In a 50 ml three-necked flask dried under reduced pressure were charged 
2.94 g (10.1 mmol) of the epoxide [trans-(7)] obtained in the above (9), 
1.30 ml (1.20 mmol) of phenyl isocyanate (PhNCO), and 30 ml of methylene 
chloride and was further added 1.81 ml (13.0 mmol) of triethylamine. The 
temperature was maintained to room temperature and stirring was conducted 
overnight. Thereafter, reaction was stopped by 1N aqueous hydrochloric 
acid solution and reaction mixture was extracted with methylene chloride. 
The methylene chloride layer was dried over anhydrous magnesium sulfate 
and solvent was distilled off under reduced pressure. The resulting 
composition was purified by silica gel column chromatography to obtain the 
above carbamate. Yield 89%. 
.sup.1 9 F-NMR: -3.5(d, J.sub.H-F =6.6 Hz); -3.1(d, J.sub.H-F =6.4 Hz) 
(11) Preparation of 
5-benzyloxy-3-(N-phenylamino)-1,1,1-trifluoropentane-2,4-diol (10) 
(a) Preparation of epoxide trans-(7) and urethane (9) 
In 100 ml eggplant type flask were charged 3.42 g (9.0 mmol) of the 
carbamate trans (8) prepared in the above (10), 1.08 g (20 mmol) of 
methoxysodium and 50 ml of methanol, followed by stirring overnight. 
Reaction was stopped by adding water and reaction mixture was extracted 
with methylene chloride. The organic layer was dried over anhydrous 
magnesium sulfate and solvent was distilled off under reduced pressure. 
The resulting crude product was purified by silica gel column 
chromatography to obtain epoxide trans-(7) and urethane (9) as a mixture. 
The two products were respectively pure compounds as diastereomers. NMR 
spectra of the resulting compounds are as follows. 
.sup.1 9 F-NMR: -0.25(d, J.sub.H-f =6.6 Hz); +1.58(d, J.sub.H-F =6.6 Hz) 
(b) Preparation of aminodiol (10) 
The mixture obtained in the above (a) was dissolved in a mixture of 30 ml 
of ethanol and 10 ml of water and the solution was charged in a 50 ml 
eggplant type flask. Thereto was added 0.06 g (30.0 mmol) of lithium 
hydroxide, followed by stirring overnight. Then, water was added to stop 
the reaction and the reaction mixture was extracted with methylene 
chloride. The organic layer was dried over anhydrous magnesium sulfate and 
concentrated. The resulting crude product was purified by silica gel 
column chromatography to isolate aminodiol (10) and epoxide trans-(7). 
Two-stage total yield: 60%. NMR spectrum of the aminodiol (10) was as 
follows. 
.sup.1 H-NMR.delta.(ppm): 3.28(bs, 1H, NHPh); 3.83 [bs, 2H, CH(OH)CH.sub.2 
O]; 3.80.about.4.20 [m, 4H, OH, OH, CHNHPh, CH(OH)CH.sub.2 O[; 4.60(s, 2H, 
CH.sub.2 Ph); 4.43-4.95(m, 3H, CF.sub.3 CH, OCH.sub.2 O); 
6.63.about.7.5(m, 5H, NHPh); 7.42(s, 5H, CH.sub.2 Ph). 
.sup.1 9 F-NMR: -2.30(d, J.sub.H-F =7.5 Hz)