Process for improving floral fertility

Improved floral fertility is obtained by applying lipoaminoacids to plants during the period between floral induction and the end of inflorescence of the plant. Foliar spraying can be used to apply a solution or aqueous suspension that has 10 to 1000 ppm of lipoaminoacids. The solution of suspension is applied in amounts of 5 to 100 g per hectare.

It is well known that floral abortion is, presently, an important problem 
in the agricultural field: a high floral abortion rate results in poor 
production yields with all the corresponding economic consequences. If 
such problem could be partially solved, it would result in a more regular 
agricultural production. 
The studies of J.C. VALLEE [These Nov. 1972, Faculte des Sciences de Dijon, 
"Quelques aspects du metabolisme de la proline sur les divers nicotiana en 
fonction du developpement"], of E. Perdrizet, J. C. Vallee, C. Martin, 
[(1967) Comptes-rendus, Academie des Sciences, Serie D, 264, 1024-1027, 
(1968) Comptes-rendus, Annales physiologie Veg., 10, 237-250, (1969) 
Comptes-rendus, Academie des Sciences, Serie D, 269, 1190-1193], and also 
of Margara, Bouniols et alii [(1971) Comptes-rendus, Academie des Sciences 
Paris, Serie D, 273, 1104-1107 and 1193-1196] demonstrated, in laboratory, 
that sugar and salt could have some action on the floral induction and 
that phenolamides and proline were involved, but this did not result in 
any carrying out in agricultural practice. 
The studies of Britikov et al., [C.A. Vol. 66, No. 5, p. 1642, (1965) 
Fiziol Razt, 12, p. 953-967, (1966) Fiziol Razt, 13, 978-987] using 
proline or anti-metabolites thereof on male sterility, improved the 
knowledge in this field without resulting in any practical application. 
Artifical technics, such as bees attraction by sugars or like compounds, as 
described by BURDEN, W.S.P. 4,075,783, do not appear to have reached a 
noticeable development. 
To date, no known techniques or means avoid or reduce floral abortion (non 
floral pollination due to weather conditions and which might be due to 
pollen sterility). In the agricultural, horticultural, sylvan, 
aquacultural fields etc . . . , there is no known process for increasing 
pollination. 
The present invention provides a means for increasing the floral fertility 
(pollination capacity, particularly under defavourable weather conditions) 
consisting of applying to the plants, during the period comprised between 
floral induction and the end of inflorescence, a specific amount of 
lipoaminoacids and/or related compounds. Specific amount means that 
quantitites brought by hectare should be comprised between 5 and 100 g and 
applied under the form of foliar sprayings of solutions or of aqueous 
suspensions, wherein lipoaminoacids or related compounds concentrations 
are comprised between 10 and 1000 ppm. 
The aim of the present invention is to improve the quality of floral 
material (stamens and pistils). The period of use is after and not before 
floral induction because the lipoaminoacids and related compounds don't 
seem to have any effect on floral induction itself but they speed up and 
enlarge floral process and, consequently, they can be used during all the 
inflorescence period. 
On certain plants, particularly peas, it may be preferably to spray the 
lipoaminoacids or related compounds right in the middle of the flowering 
period such that, even if weather conditions do not remain favourable, 
pollination can be secured up to the end of the cycle, which leads to more 
abundant and larger pods. 
Further to their action against abortion, an increase of 10 to 20% of the 
fecundated flowers in normal conditions can be expected from 
lipoaminoacids and related compounds. 
Lipoaminoacids and related compounds should be understood as described in 
Morelle French Pats. Nos. 2,403,024, 2,495,608, 2,503,144 and 2,503,153, 
the agricultural portions of which have been assigned to the applicant. 
The lipoaminoacids and related compounds are, accordingly, acyl aminoacids 
with an acylating moiety containing from 4 to 30 carbon atoms (either 
lipomonoaminoacids or polyaminoacids) and their derivatives as salts 
either formed with metals or with one or more amines, or with basic 
aminoacids. These compounds may be used alone or associated with other 
substances, whose action may be advantageous for plants. The term 
lipoaminoacids as used in the specification and claims means 
lipoaminoacids and/or related compounds which are defined as the result of 
acylation by a fatty acid comprising from 4 to 30 carbon atoms of either 
isolated naturally occurring amino acids (lipomonoaminoacids) or mixtures 
of naturally occurring amino acids obtained by hydrolysis of proteins 
(lipopolyaminoacids) and as the salts of the same with metals or with one 
or more amines or with basic amino acids. 
According to the invention, it has been found that, for each plant and each 
selected lipoaminoacid, there are accurate ranges both for the amounts to 
be used by hectare and for the concentrations of solutions or of aqueous 
suspensions applied. For instance, butyrylcollagen acid on corn, in order 
to increase the pollination and consequently the number of grains, must be 
brought preferably by foliar sprayings, from the first appearance of 
stamens, with around 20 g by hectare in 200 l of water, i.e. at a 
concentration of 100 ppm. Broadly, the amount of lipoaminoacids to be 
applied to the plants ranges between 5 to 50 g per hectare at 
concentrations between 10 to 1000 ppm. 
In these conditions, the increase of output can reach 10 or 15 quintals by 
hectare, but the results are different according to varieties of corn 
used; they are better with Pernel, Arminda or Normal than with Camp Remy. 
The close relationship between the results and the varieties is, itself, 
dependent upon the weather conditions of the year ; the experiments show 
that the fecundating action of lipoaminoacids leads to a result close to 
the maximum of the variety involved but that it does not effect the level 
of this maximum. The action on outputs, if it is closely related with the 
number of fecundated grains or berries, is also dependent upon the level 
of general fertilization by fertilizers and oligo-elements. For instance, 
the action of butyrylcollagenic acid on floral pollination can lead to a 
supplementary output only if fertilization meets the needs of the plant. 
On vines which are sensitive to abortion, and particularly grenache, 
merlot, pinot noir and chardonnay, the increase of output may frequently 
be of some thousand kilos of grapes by hectare when using butyrylcollagen 
in amounts and concentrations conditions similar to these used on corn. 
The acyl aminoacids, whose acylating moiety comprises less than 12 carbon 
atoms, have to be used in amounts by hectare comprised between 5 and 50 g 
and, preferably, sprayed under the form of solutions or aqueous 
suspensions, wherein the concentrations in lipoaminoacids are comprised 
between 10 and 1000 ppm. The doses have to be increased to 25-100 g when 
lipoaminoacids have an acylating moiety comprising more than 12 carbon 
atoms (corresponding concentrations 50 to 10 000 ppm). 
The amounts and concentrations have to be adapted to the nature of plants, 
to the varieties or to the conditions of culture but the indicated ranges 
are usually suitable for cereals, rice, maize, sunflower, peas, beans, 
vines, fruit-trees etc . . . 
The foliar spraying may be applied at the apparition of the first floral 
buds, on a vine for instance. The application may be renewed 8 days layer, 
should the flowering process not be achieved, due to bad weather 
conditions. 
To sum up, the process consists of applying lipoaminoacids, i.e. acyl 
aminoacids with an acylating moiety containing from 4 to 30 carbon atoms 
(either lipomonoaminoacids or polyaminoacids), i.e. the mixture obtained 
by acylation, by the selected acylating moiety, of a mixture of naturally 
occuring aminoacids obtained by hydrolysis of proteins, and their 
derivatives as salts either formed with metals or wtih one or more amines, 
or with basic aminoacids, preferably by foliar sprayings, in order to 
speed up and enlarge floral process; this application has to be made 
during inflorescence, with well defined amounts by hectare and 
concentrations of the solutions or suspensions in water. 
It will be noted that Morelle's U.S. Pats. Nos. 2,503,144 and No. 2,503,153 
of Apr. 2 1981 don't describe or claim any action regarding flowering, 
which is the consequence of a particular metabolic process and that U.S. 
Pat. No. 2,403,024 of 1977 only claims germination, growth, antifungic and 
antiparasitic effects, without any reference to flowering ; that is 
induced by a very specific metabolism. The total pollination depends on 
the pollen potential, which is increased in the mentioned conditions of 
use.

Examples of preparation for increasing the floral fertility 
(a) copper butyryl collagenate 0.012% ethanolamine lauroyl collagenate 
0.006% water 
(b) butyryl collagenic acid 0.006% fatty polyoxyethylen alcohol 0.003% 
water 
(c) copper caprylylkeratinate 0.08% ethanolamine lauroyl collagenate 0.04% 
water 
(d) copper caprylylglycinate 0.08% ethanolamine lauroyl collagenate 0.04% 
water 
(e) butyrylproline 0.003% water 
(f) butyrylhydroxyproline 0.003% water 
N.B.: Butyryl collagenic acid should be understood as the result of the 
acylation by butyric acid of the mixture of aminoacids obtained by the 
hydrolysis of collagen. Salts of this acid may be obtained, for instance, 
with copper and ethanolamine. The same remark applies also to 
caprylylkeratinate and lauroyl collagenate.