Low-energy scanning transmission electron microscope

Low-energy scanning transmission electron microscopy is achieved by using a sharply pointed electrode as a source of electrons having energies less than 10 eV and scanning the electron emitting pointed source across the surface of a self-supported thin film of material to be investigated at an essentially constant distance on the order of nanometers. The electrons transmitted through the specimen are sensed by a suitable detector and the output signal of the detector is used to control a display unit, such as a CRT display or a plotter. A scanning signal generating means simultaneously controls both the scanning of the electron emitting point source and the display unit while a separation control unit holds the distance between the point source and surface at a constant value. The electron emitting point source and associated mechanical drives as well as the specimen film and electron detector are all positioned in a vacuum chamber and isolated from vibration by a damped suspension apparatus.

DESCRIPTION 
1. Technical Field This invention relates to a low-energy scanning electron 
microscope for investigating the properties of self-supported thin films 
in the transmission mode. Low-energy in this context refers to electrons 
having an energy not exceeding ten electron volts. 
2. BACKGROUND ART 
In the well-known conventional transmission electron microscope the 
electron source typically is a hairpin-shaped tungsten wire which emits 
electrons when heated. The emitted electrons are accelerated through a 
potential drop typically of 40 to 100 kV and then are transmitted through 
a very thin specimen. An electron lens arranged downstream from the 
specimen generates a typically 50 to 100-fold enlarged intermediate image. 
By means of one or more additional electron lenses, the intermediate image 
is further enlarged and projected onto a screen for observation. 
In conventional electron microscopes the specimen can be damaged by 
ionizing events caused by the electrons passing through the specimen with 
energies of tens or even hundreds of keV. Indeed, in some caes, such as 
with electron beam lithography, such ionizing events have a useful 
function. However, in the investigation of protein crystals or biological 
molecules for biomedical purposes, such ionization effects cause 
catastrophic damage to the specimen and are highly undesirable. 
Unfortunately, it is not practical with a conventional electron microscope 
to reduce the energy of the electrons to a few electron volts or less 
because a very low acceleration voltage will result in an electron beam 
which cannot be suitably focussed. Either the resulting beam diameter 
would be much too large for practical use or the electron density in the 
resulting beam would be too low to be of practical use (or both). This 
situation exists with respect to the conventional transmission microscope 
both when it is operated in the conventional imaging mode and when it is 
operated in the scanning mode. 
DISCLOSURE OF THE INVENTION 
It is an object of the present invention to advance the usefulness of 
transmission electron microscopy by providing a way of avoiding the use of 
a high-energy electron beam and thereby reducing or avoiding damage to the 
specimen. 
This object and other objects have been achieved by the present invention 
in which a low-energy scanning transmission electron microscope has been 
formed. It has already been established that slow, i.e. low-energy, 
electrons can be transmitted through self-supported thin films. In an 
article entitled "Slow-Electron Beam Attenuation by Gold Films", 10 Appl. 
Phys. Lett. 73-75 (1967), H. Kanter showed that if electrons with a 1.1 eV 
energy are incident upon a self-supported gold film having a thickness of 
20 nm, one electron in 50,000 will emerge from the far side of the film 
without loss of energy. For a 5 nm gold film, Kanter's findings can be 
extrapolated to give a transmission of one in about 500. Since these are 
average values for a large-area electron beam, one might expect to find 
higher values if a low-energy electron beam could be very narrowly 
focussed to form a more intense small area low-energy electron beam. As 
previously pointed out, however, this cannot be done with a conventional 
e1ectron beam apparatus, since low-energy electron beams cannot be 
focussed to form a small area beam of practical electron density. 
In accordance with this invention, a small area of a surface is bombarded 
with low-energy electrons without any need to do focussing by emitting the 
low-energy electrons from a point source and positioning the point source 
suitably close to the film being investigated. 
An electron emitting pointed electrode is physically scanned in close 
proximity to a self-supported thin film specimen or target. The voltage 
between the film and the electrode is maintained at preferably one volt or 
less so that low-energy electrons are emitted from the electrode and 
travel towards the film. An electron sensing device is positioned on the 
opposite side of the thin film for detecting low-energy electrons which 
have been emitted by the electrode and which have passed through the thin 
film. 
When the separation between the pointed electrode and the film is less than 
about 1 nm, the electron clouds of the atoms at the apex of the pointed 
electrode and at the surface opposite the apex touch, and a tunnel current 
path is established between the apex of the pointed electrode and the 
film. When the separation is more than about 3 nm, electrons must leave 
the pointed source via field emission. When the separation is between 
about 1 and 3 nm, both current effects are experienced. In any case, the 
area of the surface receiving the electrons has a diameter roughly equal 
to the distance between the point source and the specimen. 
In the prior art, low-energy electrons also have been emitted from a point 
source positoned close to a surface in the Scanning Tunneling Microscope, 
described by G. Binnig et al., for example, in the article entitled, 
"Surface Studies by Scanning Tunneling Microscopy", 49 Phys. Rev. Lett. 
57-61 (1982), and in the article entitled, "Tunneling Through a 
Controllable Vacuum Gap," 40 Appl. Phys. Lett. 178-180 (1982), and in U.S. 
Pat. No. 4,343,993. Low-energy electrons have also been emitted from a 
point source positioned close to a surface in the apparatus described by 
R. D. Young in "Field Emission Ultramicrometer", 37 Rev. Sci. Instrum. 
275-278 (1966). 
While it may be observed in retrospect that these prior art devices 
incidently irradiate a small surface area with low-energy electrons, these 
devices were not used to do transmission electron microscopy but rather 
were used to measure distance or height. In the present invention 
low-energy electrons from a point electron source are being applied to a 
thin film for the purpose of transmitting some of the electrons through 
the film for collection by an electron detector. The specimen being 
irradiated with the prior art devices was not in the form of a thin film. 
Low-energy electrons did not pass through the prior art specimen. In the 
prior art devices the effect that the proximate surface had upon the 
emission characteristics of the source was used to control or measure the 
position of the electron source with respect to the surface for the 
purpose of measuring the position or height of the proximate surface. No 
known prior art uses a point electron source positioned very close to a 
surface for the direct and sole purpose of applying low-energy electrons 
to a small area of the surface rather than detecting the position or 
height of the surface.

DESCRIPTION OF THE PREFERRED EMBODIMENTS 
FIG. 1 is a diagram showing the range through which electrons can travel 
between scattering events, with relation to their energy. The diagram 
shows the existence of a "low-energy window" for electrons with energies 
typically below 10 eV. 
Referring now to FIG. 2, for the electrons to arrive at the surface 2 of 
the material 3 to be investigated, the distance between the apex of the 
electron emitting point 1 and surface 2 must be on the order of 1 nm for 
an energy of about 10 eV. Two operating modes can be distinguished. First, 
for a distance greater than approximately 3 nm, the electrons leave point 
1 by field emission. Second, for a distance shorter than approximately 1 
nm, the electrons leaving point 1 will tunnel through that distance. In 
the tunneling mode, the energy of the electrons is less than 1 eV. In the 
field emission mode, the energy of the electrons increases with the 
distance. 
The material to be investigated takes the form of a self-supported thin 
film 3, i.e. the material is mounted across a hole in a support 6 as shown 
more clearly in FIG. 3. Of the electrons 4 incident upon surface 2 of thin 
film 3, some electrons will be transmitted through the material either 
with or without loss of energy. These transmitted electrons are detected 
by a conventional electron detector 5 arranged on the opposite side of 
film 3. 
The arrangement of FIG. 2 shows transmission of electrons 4 through one 
particular spot of the specimen 3. Various ways are known in the prior art 
for scanning the specimen in a regular fashion so as to form an image of 
the entire specimen. In the conventional scanning electron microscope the 
scanning action is obtained by way of appropriately deflecting the 
electron beam on its way from the electron gun (or field emission source) 
to the specimen. A cathode ray tube is scanned in synchronism with the 
beam deflection electronics, such as by driving it from the same scan 
generator, so that each beam position on the specimen corresponds to a 
unique position on the cathode ray tube. 
In contrast to the conventional scanning electron microscope, the 
microscope in accordance with the present invention requires a mechanical 
scanning system since the emitted electrons here travel only through a 
distance of a few nanometers (at most), which does not permit any 
deflection of the electrons or space for any deflection coils or 
electrodes. A rather simple mechanical deflection mechanism may be used 
which employs, e.g. lead screws for coarse positioning of the electrode 1 
with respect to film 3, and piezoelectric elements for fine positioning 
and scanning. Mechanical positioning and scanning apparatus suitable for 
this purpose has been described by Binnig et al. in the articles and 
patent cited earlier, which are hereby fully incorporated by reference. 
Another suitable piezoelectric XY translator is described in IBM Technical 
Disclosure Bulletin Vol. 26, No. 10A, March 1984, at pages 4898-4899. 
These prior art mechanical positioning devices permit a one-to-one 
correspondence to be established between each point on the specimen or 
film 3 and each point on the cathode ray tube. It will be obvious to those 
of ordinary skill in this art that instead of a cathode ray tube display a 
graphical recorder or a similar display device such as a plotter can be 
used so long as the mentioned one-to-one relationship between locations on 
the specimen and on the image is maintained. 
As the electron emitting point source scans across the specimen each spot 
on the specimen is exposed to electrons for some fixed length of time 
(dwell time) which is determined by the speed of the scan and size of the 
irradiated area. During this dwell time (or a part thereof), the electrons 
interact with the specimen and the number of transmitted electrons depends 
on the characteristics of the specimen at that particular scanning 
location. The transmitted electrons are sensed by an appropriate detector 
(either individually by "pulse counting" techniques or collectively as an 
electron current), and the signals formed by the detector are suitably 
amplified and used, for example, to control the brightness of a cathode 
ray tube display for that particular scanning location or to give a bright 
dot for each electron that is detected or to control the instantaneous 
print density of a plotter or to plot a single line scan or an array of 
line scans. It should be apparent to one of ordinary skill in this art, 
furthermore, that the signals formed by the detector (or an amplified 
version thereof) alternatively or additionally could be stored for later 
conversion to pictorial representation or for used by a computer capable 
of doing image processing. 
Detectors useful for monitoring transmitted electrons are known in the art. 
One example is the Everhart-Thornley detector described in J. Sci. Instr., 
Vol. 37, at page 246 (1960). Any secondary electron detector which can be 
used in a scanning electron microscope also should be suitable for this 
purpose. 
A microscope in accordance with the present invention is schematically 
shown in FIG. 3. An electron emitting point source 1, a holder 14 for a 
thin film 3 of the material to be investigated, and an electron detector 5 
are supported (directly or indirectly via one or more mechanical drives) 
from a common frame 10 within a vacuum chamber 12 (which may be part of an 
existing scanning electron microscope). 
Source 16 maintains either a constant voltage or a constant current across 
a small gap 18 separating the electron emitting point source 1 and the 
thin film 3. In the constant voltage mode of operation, for example, the 
potential of electrode 1 may be maintained by source 16 at about 0.5 to 
about 10 volts negative with respect to the potential of the thin film 3. 
A mechanical scanning mechanism is associated either with the electrode 1 
or with the specimen holder 14, whichever is most convenient. In FIG. 3, 
an X,Y mechanical scanning mechanism 20 is mounted directly upon frame 10 
and controls the motion of electrode 1 in the X and Y directions via an 
arm 22. The electrode 1 is carried by the Z direction mechanical scanning 
mechanism 24, which is in turn mounted also on the frame 10. Dynamic 
control of the Z position, in addition to the scan directions X,Y, allows 
the distance between electrode 1 and surface 2 of the specimen to be 
maintained constant despite any possible roughness of the surface. An X,Y 
scan generator 26 provides signals to X,Y drive 20 for scanning point 1 
across surface 2 along the X and Y coordinates. Simultaneously, a 
separation control unit 28 responding to sensed signals characteristic of 
the actual separation between the surface 2 and the electrode 1 develops a 
Z signal for separation control by the Z drive 24. 
If source 16 maintains a constant voltage across gap 18, then separation 
control unit 28 may respond to the amplitude of the current flowing 
between the surface 2 and electrode 1, since at constant voltage this 
current becomes larger if the separation between surface 2 and electrode 1 
is reduced. This current may be detected indirectly (as illustrated) by 
monitoring the amount of current flowing to the specimen (or specimen 
holder) with a current amplifier 30. Alternatively, the current flowing 
from electrode 1 to the constant current source 14 may be directly 
monitored (not illustrated). 
If source 16 instead maintains a constant current across gap 18, then 
separation control unit 28 may respond to the voltage across gap 18 (not 
shown), since at constant current this voltage depends upon the separation 
between electrode 1 and surface 2. 
Detector 5 collects electrons which pass through film 3 and provides either 
an amplitude signal or a series of pulses to amplifier 32 for display unit 
34. Display 34 is scanned in synchronism with the scanning of point 1 
since both receive their scanning signals from the same scan generator 26. 
Display 34 may be a cathode ray tube or a graphic output device such as a 
plotter or similar device. In view of the fact that electrode 1 is scanned 
over surface 2 at a separation distance in the nanometer range, and that 
the resolution attainable with a piezoelectric X-Y translation mechanism 
also is on the same order of maqnitude, it is important that the electrode 
1 and specimen 3 be isolated from external sound and vibration. This may 
be achieved, for example by mounting everything inside of vacuum chamber 
12 on a damped suspension apparatus 36. The damped suspension apparatus 
may simply consist of a stack of plates 38 separated by elastic members 
40. In order to provide for absorption of vibrations of different 
frequencies, the cross-sections and/or elasticities of the elastic members 
40 may vary along the stack.