Automatic analyzing apparatus

An automatic analyzing apparatus includes a stir piece moving part that moves a stir piece for stirring a mixture into a reaction container, a reaction disk that rotationally moves and thereafter stops the reaction container in which the stir piece moved by the stir piece moving part and the mixture are housed, and first drivers that drive the stir piece in the reaction container to stir first and second mixtures. During the rotation movement of the reaction container into which first and second reagents have been dispensed at first and second reagent dispensing positions, the first drivers vertically move the stir piece in the reaction container and stir the first and second mixtures.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates to an automatic analyzing apparatus that analyzes components contained in fluid and a stirring method thereof, more specifically, relates to an automatic analyzing apparatus that analyzes chemical components contained in blood, urine, etc., of human.

2. Description of the Related Art

An automatic analyzing apparatus covers biochemical test items and immunoserological test items. The automatic analyzing apparatus dispenses a test sample and a reagent corresponding to a test item for the test sample into a reaction container, and measures the change in color tone and turbidity due to reaction of a mixture by measurement of transmission light, thereby obtaining the densities and enzyme activities of various components in the test sample.

This automatic analyzing apparatus measures a test item selected for a test from among a plurality of test items that are measurable based on the setting of analysis conditions for each test sample. When the rotationally moving reaction container stops, a sample-and-reagent dispensing probe dispenses the sample and a reagent for the test item into the reaction container. After the sample and the reagent are dispensed, a stir piece stirs the mixture of the sample and the reagent in the reaction container when the reaction container stops. After the mixture is stirred, a photometric part measures the mixture in the rotationally moving reaction container. The sample-and-reagent dispensing probe is cleaned every time dispensing ends, the stir piece is cleaned every time stirring ends, and the reaction container is cleaned every time mixture measurement ends. The sample-and-reagent dispensing probe, the stir piece, and the reaction container are repeatedly used for measurement every time cleaning ends.

Meanwhile, a method for stirring a mixture is, for example, moving a stir piece attached to the tip of a motor from above into a reaction container while the reaction container is stopping, and rotating the stir piece to stir a mixture in the reaction container. Moreover, a method of moving a stir piece disposed to the tip of a thin metal plate, on whose both faces piezoelectric elements are bonded, from above into a reaction container and vibrating the stir piece to stir a mixture in the reaction container is also known (Japanese Patent No. 3135605).

However, the automatic analyzing apparatus required to process a number of tests (obtained by multiplying the number of test samples by the number of test items) at high speeds cannot sufficiently stir a mixture while a reaction container is stopping in a case where, for example, the mixture contains a test sample or reagent with high viscosity. Consequently, a problem of deterioration of analysis data arises.

SUMMARY OF THE INVENTION

The present invention has been devised to solve the above problem, and makes it possible to increase the stir performance. An object of the present invention is to provide an automatic analyzing apparatus and a stirring method thereof that can prevent deterioration of analysis data due to insufficient stir.

In a first aspect of the present invention, an automatic analyzing apparatus comprises: a stir piece housed so as to be vertically movable in a reaction container housing a mixture of a test sample and a reagent; a reaction container moving part configured to move the reaction container housing the stir piece and the mixture; and a driver configured to stir the mixture by alternately driving the stir piece in the reaction container upward and downward during movement of the reaction container by the reaction container moving part.

According to the first aspect, the mixture is stirred during the movement of the reaction container. Therefore, it is possible to respond to a request for processing a number of tests at high speeds. Moreover, since the stir piece is alternately driven upward and downward, the test sample does not accumulate on the bottom of the reaction container but mixes with the reagent. Therefore, it becomes possible to increase the stir performance, and prevent deterioration of analysis data due to insufficient stir.

Further, in a second aspect of the present invention, an automatic analyzing apparatus comprises: a stir piece, which is a permanent magnet, housed so as to be vertically movable in a reaction container housing a mixture of a test sample and a reagent; and a driver placed below the reaction container, provided with a magnet whose facing plane is formed so as to be capable of facing the reaction container, and configured to relatively move the reaction container and the facing plane of the magnet so that the facing plane of the magnet indicates either an north pole or a south pole to draw the stir piece downward and the facing plane of the magnet indicates the other pole to repulse the stir piece upward.

According to the second aspect, the stir piece is alternately driven upward and downward. Therefore, it becomes possible to increase the stir performance, and prevent deterioration of analysis data due to insufficient stir. Moreover, use of a magnet as the stir piece makes it possible to vertically drive the stir piece by magnetic power with efficiency, and moreover, it becomes possible to install the driver in a small space.

Further, in a third aspect of the present invention, the automatic analyzing apparatus according to the first aspect has a photometric part configured to apply light to the reaction container and measure light transmitted through the mixture in the reaction container. A lower face and an upper face of the stir piece have similar shapes smaller than a bottom face inside the reaction container open-topped and formed into a hollow cylindrical column or polygonal column. A part of a side face of the stir piece facing an inner wall of the reaction container transmitting light is contracted so as not to contact the inner wall.

Further, in a fourth aspect of the present invention, the automatic analyzing apparatus according to the second aspect has a photometric part configured to apply light to the reaction container and measure light transmitted through the mixture in the reaction container. A lower face and an upper face of the stir piece have similar shapes smaller than a bottom face inside the reaction container open-topped and formed into a hollow cylindrical column or polygonal column. A part of a side face of the stir piece facing an inner wall of the reaction container transmitting light is contracted so as not to contact the inner wall.

Further, in a fifth aspect of the present invention, the automatic analyzing apparatus according to the first aspect has at least one piercing hole that pierces the lower face and the upper face so that the mixture in the reaction container can flow from a lower side of the lower face to an upper side of the upper face and vice versa when the stir piece vertically moves in the reaction container.

Further, in a sixth aspect of the present invention, the automatic analyzing apparatus according to the second aspect has at least one piercing hole that pierces the lower face and the upper face so that the mixture in the reaction container can flow from a lower side of the lower face to an upper side of the upper face and vice versa when the stir piece vertically moves in the reaction container.

Further, in a seventh aspect of the present invention, the automatic analyzing apparatus according to the second aspect is characterized in that the magnet is placed on part of an orbit on which the reaction container moves.

Further, in an eighth aspect of the present invention, the automatic analyzing apparatus according to the second aspect is characterized in that the magnet of the driver is an electromagnet.

Further, in a ninth aspect of the present invention, an automatic analyzing apparatus comprises: a stir piece, which is a permanent magnet, housed so as to be vertically movable in a reaction container housing a mixture of a test sample and a reagent; an electromagnet configured to alternately drive the stir piece in the reaction container upward and downward; an electric power supplying part configured to supply an electric current whose polarity is alternately changed to the electromagnet; and a controller configured to control the electric power supplying part based on a liquid property or an amount of one or both of the test sample and the reagent contained in the mixture in the reaction container, and vary a level or frequency of the electric current supplied to the electromagnet or a level and frequency of the electric current supplied to the electromagnet.

According to the ninth aspect, in accordance with the liquid property or the amount of one or both of the test sample and the reagent container in the mixture in the reaction container, the number of times and speed of stir of the mixture is varied. Therefore, it becomes possible to increase the stir performance, and prevent deterioration of analysis data due to insufficient stir. Moreover, it becomes possible to install the driver in a small space.

DETAILED DESCRIPTION OF THE EMBODIMENTS

An embodiment of an automatic analyzing apparatus according to the present invention will be described below with reference toFIGS. 1 to 12.

FIG. 1is a block diagram showing the configuration of the automatic analyzing apparatus according to the embodiment of the present invention. An automatic analyzing apparatus100is provided with: an analyzer18configured to measure standard samples or test samples of various test items by using a reagent for every test item; an analysis controller40configured to drive and control each unit used in measurement by the analyzer18; and a data processor50configured to process standard sample data or test sample data outputted from the analyzer18after measurement of the standard samples or the test samples to create a calibration curve or generate analysis data.

Further, the automatic analyzing apparatus100is provided with: an output part60configured to output the calibration curve created by the data processor50or the analysis data generated thereby; an operation part70for inputting analysis conditions for the respective test items, various command signals, etc.; and a system controller80configured to integrally control the analysis controller40, the data processor50, and the output part60.

FIG. 2is a perspective view showing the configuration of the analyzer18. The analyzer18is provided with: a sample container17configured to house samples such as the standard samples and the test samples; a disk sampler5configured to hold the sample container17housing the samples so as to be rotatable; a sample dispensing probe16configured to execute a dispensing operation of sucking the sample from the sample container17and discharging it to a reaction container3in every analysis cycle; and a sample dispensing arm10configured to hold the sample dispensing probe16so as to be rotatable and vertically movable.

Further, the analyzer18is provided with: a reagent container6configured to house a first reagent that reacts with a component of each of the test items included in the sample; a first reagent storage1in which a rack1afor rotatably holding the reagent container6housing the first reagent is stored; a first reagent dispensing probe14configured to, in every analysis cycle, execute a dispensing operation of sucking the first reagent from the reagent container6in the first reagent storage1and discharging it to the reaction container3into which the sample has been dispensed; and a first reagent dispensing arm8configured to hold the first reagent dispensing probe14so as to be rotatable and vertically movable.

Furthermore, the analyzer18is provided with: a sample container7configured to house a second reagent paired with the first reagent; a second reagent storage2in which a rack2afor rotatably holding the reagent container7housing the second reagent is stored; a second reagent dispensing probe15configured to, in every analysis cycle, execute a dispensing operation of sucking the second reagent from the reagent container7in the second reagent storage2and discharging it to the reaction container3into which the sample and the first reagent have been dispensed; and a second reagent dispensing arm9configured to hold the second reagent dispensing probe15so as to be rotatable and vertically movable.

Furthermore, the analyzer18is provided with: a reaction disk4configured to rotationally move, by a predetermined angle θ1, a plurality (m pieces) of reaction containers3arranged at equal intervals on the circumference in every analysis cycle and then stop them; a stirring part11configured to stir, in every analysis cycle, a first mixture composed of the sample and the first reagent discharged into the reaction container3or a second mixture composed of the sample, the first reagent, and the second reagent; a photometric part13configured to measure the first mixture or the second mixture within the reaction container3; and a cleaning unit12configured to hold, so as to be vertically movable, a cleaning nozzle for sucking the first mixture or the second mixture after measurement in the reaction container3and cleaning the inside of the reaction container3, and a drying nozzle for drying the inside of the reaction container3.

The photometric part13applies light to the rotationally moving reaction container3at a photometric position, converts the light transmitted through the mixture containing the standard sample into absorbance to generate the standard sample data, and thereafter outputs it to the data processor50. Moreover, the photometric part13converts the light transmitted through the mixture containing the test sample into absorbance to generate the test sample data, and thereafter outputs it to the data processor50. The reaction container3, sample dispensing probe16, first and second reagent dispensing probes14and15and stirring part11after measurement, i.e., the respective units having contacted liquids such as the sample, the first reagent, the second reagent, the first mixture and the second mixture, are cleaned and thereafter used in measurement again.

The analysis controller40is provided with a mechanism part41having mechanisms for driving the respective units of the analyzer18, and a controller42configured to drive the respective mechanisms of the mechanism part41and control the respective units of the analyzer18.

The mechanism part41is provided with: mechanisms configured to rotate the rack1aof the first reagent storage1, the rack2aof the second reagent storage2, and the disk sampler5, respectively; a mechanism configured to rotate the reaction disk4; mechanisms configured to rotate and vertically move part of the units including the sample dispensing arm10, first reagent dispensing arm8, second reagent dispensing arm9and stirring part11, respectively; and a mechanism configured to vertically move the cleaning unit12.

Further, the mechanism part41is provided with: a mechanism configured to drive a sample dispensing pump to suck and discharge, the sample dispensing pump causing the sample dispensing probe16to suck and discharge the sample; mechanisms configured to drive first and second reagent pumps to suck and discharge, respectively, the first and second reagent pumps causing the first and second reagent dispensing probes14and15to suck and discharge the first and second reagents; a mechanism configured to drive a cleaning pump that sucks the first mixture or the second mixture from the cleaning nozzle of the cleaning unit12or that discharges and sucks a cleaning solution; a mechanism configured to drive a drying pump that sucks from the drying nozzle of the cleaning unit12; and so on.

The data processor50shown inFIG. 1is provided with: a calculator51configured to create a calibration curve or generate analysis data from standard sample data or test sample data outputted from the analyzer18; and a data storage52configured to store the calibration curve created by the calculator51, the analysis data generated thereby, etc.

The calculator51creates a calibration curve from the standard sample data of each test item outputted from the photometric part13of the analyzer18, and stores it into the data storage52and also outputs it to the output part60. Moreover, the calculator51reads out a calibration curve of a test item corresponding to the test sample data outputted from the photometric part13of the analyzer18, from the data storage52.

Next, the calculator51generates analysis data such as the density and active value of a test item component from the test sample data by using the calibration curve having been read out, and stores the generated analysis data into the data storage52and also outputs it to the output part60.

The data storage52is provided with a hard disk, etc. The data storage52stores the calibration curve outputted from the calculator51for every test item, and stores the analysis data of the respective test items outputted from the calculator51for every test sample.

The output part60is provided with a printing part61configured to print out the calibration curve, the analysis data, etc., outputted from the data processor50, and a display62configured to display them. The printing part61is provided with a printer, etc. The printing part61prints out the calibration curve, the analysis data, etc., outputted from the data processor50to printing sheets in accordance with preset formats.

The display62is provided with a monitor such as a CRT and a liquid crystal display. The display62displays the calibration curve or analysis data outputted from the data processor50. The display62also displays: an analysis condition setting screen for setting analysis conditions such as the amounts of the sample, first reagent and second reagent and the wavelength for each test item; a subject information setting screen for setting the ID and name of a subject, etc.; a measurement item selecting screen for selecting a test item to measure for each test sample; and so on.

The operation part70is provided with an input device such as a keyboard, a mouse, a button, and a touch screen. The operation part70is used for inputting subject information such as a subject ID and a subject name, a test item to measure for each test sample, and the like.

The system controller80is provided with a CPU and a memory circuit. The system controller80stores a command signal supplied from the operation part70, analysis conditions for each test item, the subject information, and information such as a test item to measure for each test sample. Then, the system controller80controls the entire system based on these information.

Next, with reference toFIGS. 1 to 9, the configuration and operation of the stirring part11in the analyzer18will be described.

FIG. 3is a top view showing the configuration of the stirring part11. The stirring part11is placed on the outer periphery of the reaction disk4of the analyzer18. The stirring part11is provided with a stir piece20for stirring the first mixture or the second mixture in the reaction container3, a housing part21that houses the stir piece20, a stir piece moving part22for moving the stir piece20housed by the housing part21into the reaction container3, a driver23for driving the stir piece20in the reaction container3to stir the first mixture or the second mixture, and a stir piece cleaning part24for cleaning the stir piece20having been for stirring the first mixture or the second mixture.

FIG. 4is a view showing an example of the stir piece20in the reaction container3. For example, the stir piece20is a permanent magnet that indicates either the north pole or the south pole on a lower face201and the other pole on an upper face202and that can vertically move in the reaction container3. The stir piece20is coated with a material having high chemical resistance and small frictional coefficient, such as polytetrafluoroethylene.

The lower face201and the upper face202are similar in shape, which are slightly smaller than the bottom face of the inside of the reaction container3that is open-topped and formed into a hollow quadrangular column. A part of a side face203facing the inner wall of the reaction container3transmitting light is constricted so as not to contact a light-transmitting part of the inner wall. Moreover, the side face203has a specified height so that the lower face201and the upper face202cannot be reversely positioned in the reaction container3, and the stir piece20is housed in the reaction container3so as to be vertically movable. Besides, a plurality of piercing holes204that pierce the lower face201and the upper face202are formed so that the first mixture or the second mixture can flow from either the lower side of the lower face201or the upper side of the upper face202to the other and can be stirred when the stir piece20vertically moves in the reaction container3.

The configuration of the stir piece is not limited to the above example, and the stir piece may be a rectangular stir piece20awhose lower and upper faces are along the bottom face of the inside of the reaction container3as shown inFIG. 5. Moreover, as shown inFIG. 6, the stir piece may be a stir piece20bwhose lower and upper faces are circular. Besides, the stir piece may be a stir piece whose lower and upper faces have similar shapes slightly smaller than the bottom face of the inside of the reaction container that is open-topped and formed into a hollow cylindrical column or polygonal column other than quadrangular column.

The housing part21ofFIG. 3houses a plurality of stir pieces20with upper faces202facing above so as to be rotatable. Moreover, the stir piece20cleaned in the stir piece cleaning part24is dried.

FIG. 7is a view showing the configuration of the stir piece moving part22. The stir piece moving part22is controlled by the analysis controller40. The stir piece moving part22includes an absorption/desorption probe221that absorbs and desorbs the stir piece20at the lower end thereof, and a moving arm222that holds the upper end of the absorption/desorption probe221so as to be vertically movable and rotatable.

For example, the absorption/desorption probe221has an electromagnet in the lower end thereof. As shown inFIG. 8A, the absorption/desorption probe221moves into the housing part21, approaches and absorbs the upper face202of an unused or dried stir piece20by electric power supplied to the electromagnet from the controller42of the analysis controller40, and thereafter desorbs the absorbed stir piece20within the reaction container3stopping at a distributing position as the controller42stops supplying electric power to the electromagnet.

Further, as shown inFIG. 8B, the absorption/desorption probe221moves into the reaction container3after measurement stopping at the distributing position, absorbs the stir piece20by electric power supplied to the electromagnet, and thereafter desorbs the absorbed stir piece20within the stir piece cleaning part24as the controller42stops supplying electric power to the electromagnet. In the stir piece cleaning part24, a mixture adhering to the stir piece20is washed away by using alkaline detergent, acid detergent, cleaning solution containing surface active agent, etc., or pure water. The mixture may be washed away by simultaneously using ultrasonic cleaning. Furthermore, the absorption/desorption probe221moves into the stir piece cleaning part24, absorbs the cleaned stir piece20, and thereafter desorbs the absorbed stir piece20within the housing part21.

The stir piece20may be moved in a state where the plurality of piercing holes204or the margins of the stir piece20are sandwiched by an absorption/desorption probe provided with a robot arm mechanism near the lower end thereof.

The moving arm222is vertically moved and rotated by the mechanism part41of the analysis controller40to move the absorption/desorption probe221from the inside of the housing part21to the inside of the reaction container3stopping at the distributing position. Moreover, the moving arm222moves it from the inside of the reaction container3stopping at the distributing position to the inside of the stir piece cleaning part24. Besides, the moving arm222moves it from the inside of the stir piece cleaning part24to the inside of the housing part21.

The driver23ofFIG. 3is provided with: a first driver25that drives the stir piece20in the reaction container3to stir the first mixture while the reaction container3into which the first reagent has been dispensed at a first reagent dispensing position is rotationally moving in an arrow R1direction; and a second driver26that drives the stir piece20in the reaction container3to stir the first mixture while the reaction container3after stir by the first driver25is stopping at a first stirring position. Moreover, the driver23is provided with: a first driver27that drives the stir piece20in the reaction container3to stir the second mixture while the reaction container3into which the second reagent has been dispensed at a second reagent dispensing position is rotationally moving; and a second driver28that drives the stir piece20in the reaction container3to stir the second mixture while the reaction container3after stir by the first driver27is stopping at a second stirring position.

FIG. 9is a view showing the configuration of the first and second drivers25and26. The first driver25has: first magnets25alto25an, which are n (2<n<m) pieces of electromagnets whose ends facing up can be magnetized to the same pole as the one pole of the lower face201of the stir piece20; and second magnets25blto25bn, which are n pieces of electromagnets whose ends facing up can be magnetized to the opposite pole. The first magnets25alto25anand the second magnets25blto25bnare alternately arranged, and are magnetized by electric power (direct current) supplied from the controller42of the analysis controller40at a timing when the reaction container3into which the first reagent has been dispensed passes over the respective magnets.

Further, the first driver25is arranged below an orbit on which the reaction container3rotationally moves, in a range of an angle θ2included in the range of the predetermined angle θ1between the first reagent dispensing position at which the first reagent is dispensed into the reaction container3by the first reagent dispensing probe14and the first stirring position at which the reaction container3stops after rotationally moving from the first reagent dispensing position in one analysis cycle, as shown inFIG. 3.

Next, with reference toFIGS. 2 to 9, the operation of stirring the first mixture within the reaction container3will be described.

When the reaction container3cleaned and dried by the cleaning unit12of the analyzer18stops at the distributing position, the stir piece moving part22moves the stir piece20in the housing part21to the inside of the reaction container3. After the stir piece20is moved to the inside of the reaction container3, the sample dispensing probe16dispenses the sample from the sample container17into the reaction container3when the reaction container3housing the stir piece20stops at a sample dispensing position. After the sample is dispensed, the first reagent dispensing probe14dispenses the first reagent from the reagent container6in the first reagent storage1to the inside of the reaction container3when the reaction container3housing the sample stops at the first reagent dispensing position.

The reaction container3, into which the first reagent has been dispensed at the first reagent dispensing position, rotationally moves in the arrow R1direction. The stir piece20in the reaction container3is repulsed by the first magnet25alplaced below a one-pitch position rotationally moved by one pitch from the first reagent dispensing position and is moved upward, and thereafter, is absorbed by the second magnet25blplaced below a two-pitch position rotationally moved by two pitches and is moved downward. Here, one pitch is equivalent to a rotation angle obtained by dividing 360 degrees by m, which is the number of the reaction containers3.

Next, after being repulsed by the first magnet25a2placed below a three-pitch position rotationally moved by three pitches and being moved upward, the stir piece20is absorbed by the second magnet25b2placed below a four-pitch position rotationally moved by four pitches and is moved downward.

Furthermore, after being repulsed by the first magnet25anplaced below a (2nn−1)-pitch position rotationally moved by (2n−1) pitches and being moved upward, the stir piece20is absorbed by the second magnet25bnplaced below a 2n-pitch position rotationally moved by 2n pitches, and is moved downward, where the stir piece20stops.

The first driver25may be configured in a manner that: either the first magnets or the second magnets are lined at consecutive-two-pitch positions and the other magnets are lined at one-pitch positions or at consecutive-two-pitch positions; and then the former magnets lined at the two-pitch positions and the latter magnets lined at the one-pitch positions or at the consecutive-two-pitch positions are alternately arranged.

Thus, during the rotation movement of the reaction container3into which the first reagent has been dispensed at the first reagent dispensing position, it is possible to vertically move the stir piece20in the reaction container3to stir the first mixture.

The second driver26has a third magnet26a, which is an electromagnet whose end facing up can be alternately magnetized to one pole and the other pole, i.e., the north pole and the south pole. Then, while the reaction container3with the first reagent dispensed is stopping at the first stirring position, the third magnet26ais alternately magnetized to the one pole and the other pole by specified frequency of alternate current supplied from the controller42, and vertically moves the stir piece20in the reaction container3.

The reaction container3with the first reagent dispensed stops at the first stirring position after rotation movement to the 2n-pitch position. The stir piece20in the reaction container3repeatedly performs the operation of being repulsed by the third magnet26ato move upward and thereafter being absorbed thereby to move downward.

It is also possible to configure so as to move the stir piece into the reaction container3stopping at the first stirring position from above and thereafter rotate or vibrate the moved stir piece to stir the first mixture in the reaction container3.

Thus, it is possible to vertically move the stir piece20in the reaction container3and stir the first mixture while the reaction container3with the first reagent dispensed at the first reagent dispensing position is stopping after the rotation movement.

Consequently, it becomes possible to stir the first mixture in the reaction container3while the reaction container3is rotationally moving and while the reaction container3is stopping after the rotational movement, so that it is possible to stir the first mixture for a long time period.

The first driver27is arranged below the orbit on which the reaction container3, into which the second reagent has been dispensed by the second reagent dispensing probe15at the second reagent dispensing position, rotationally moves, and is configured in a similar manner as the first driver25. Therefore, a description thereof will be omitted. Then, the respective magnets composing the first driver27are magnetized by direct current supplied from the controller42at a timing when the reaction container3with the second reagent dispensed passes over the respective magnets. The stir piece20in the reaction container3is vertically moved to stir the second mixture.

Thus, during the rotational movement of the reaction container3into which the second reagent has been dispensed at the second reagent dispensing position, it is possible to vertically move the stir piece20in the reaction container3and stir the second mixture.

Since the second driver28is arranged below the second stirring position at which the reaction container3with the second reagent dispensed stops after rotationally moving and is configured similarly to the second driver26, a description thereof will be omitted. Then, while the reaction container3with the second reagent dispensed is stopping at the second stirring position, the magnet composing the second driver28vertically moves the stir piece20in the reaction container3by specified frequency of alternate current supplied from the controller42to stir the second mixture.

Thus, it is possible to, while the reaction container3with the first reagent dispensed at the second reagent dispensing position is stopping after the rotational movement, vertically move the stir piece20in the reaction container3and stir the second mixture. Consequently, it becomes possible to stir the second mixture in the reaction container3while the reaction container3is rotationally moving and while the reaction container3is stopping after the rotational movement, so that it is possible to stir the second mixture for a long time period.

The stir piece cleaning part24shown inFIG. 3cleans the stir piece20moved by the stir piece moving part22from the reaction container3containing the first mixture or the second mixture, and a part of the absorption/desorption probe221contacted the first mixture or the second mixture during the movement of the stir piece20. Then, the stir piece20cleaned by the stir piece cleaning part24is moved by the stir piece moving part22to the housing part21, and thereafter, is dried in the housing part21and used for measurement again.

The stir piece cleaning part24may be replaced with a stir piece collecting box so that the stir piece20in the reaction container3having been used for measurement is collected and discarded into the collecting box.

With reference toFIGS. 1 to 12, an example of the operation of the automatic analyzing apparatus100will be described below.FIG. 10is a view showing an example of the analysis condition setting screen displayed on the display62.FIG. 11is a view showing an example of the measurement item selecting screen displayed on the display62.FIG. 12is a flowchart showing the operation of the automatic analyzing apparatus100.

InFIG. 10, an analysis condition setting screen63includes fields like “item name,” “sample amount,” “reagent amount,” “wavelength” and “photometric point” and dialogue boxes631to638for setting analysis conditions in the respective fields. When an input operation into each of the dialogue boxes corresponding to the fields of the analysis condition setting screen63is performed for each test item through the operation part70, input information on the inputted analysis conditions for each test item is stored into the memory circuit of the system controller80.

In the “item name” field, the name of a desired test item is selected and set from among a plurality of test items set in advance. For example, through an operation of selecting and inputting aspartate aminotransferase as the name of a test item, “AST,” which is the abbreviated name of aspartate aminotransferase, is displayed in the dialogue box631.

In the “sample amount” field, the amount of a sample dispensed into the reaction container3at the time of measurement of the test item set in the “item name” field is set. For example, through an operation of inputting 5 μL as the sample amount, “5” is displayed in the dialogue box632.

In the “reagent amount” field, a “first reagent” field and a “second reagent” field are displayed. In a case where a reagent used for measurement of the test item set in the “item name” field is a one-reagent type, the amount of the first reagent dispensed into the reaction container3is set in the “first reagent” field. In the case of a two-reagent type, the amounts of the first and second reagents dispensed into the reaction container3are set in the “first reagent” field and the “second reagent” field. Then, through an operation of inputting 150 μL as the amount of the first reagent of the two-reagent type, “150” is displayed in the dialogue box633. Moreover, through an operation of inputting 50 μL as the amount of the second reagent, “50” is displayed in the dialogue box634.

In the “wavelength” field, a “wavelength1” field and a “wavelength2” field are displayed, and a wavelength appropriate for the kind of reaction of the test item set in the “item name” field is set.

One wavelength or two different wavelengths are selected and set from among wavelengths set in advance.

Then, for example, through an operation of selecting and inputting 340 nm, which is appropriate for the kind of reaction of the test item set in the “item name” field, into the “wavelength1” field, “340” is displayed in the dialogue box635. Moreover, for example, through an operation of selecting and inputting 380 nm into the “wavelength2” field, “380” is displayed in the dialogue box636.

In the “photometric point” field, for example, through an operation of selecting and inputting twentieth to twenty-ninth photometric points as the timings for measuring a mixture for the test item set in the “item name” field, “20” is displayed in the dialogue box637, and “29” is displayed in the dialogue box638.

Here, for example, a time when the reaction container3with the first reagent dispensed rotationally moves and first passes through a photometric position between the sample dispensing position and the first reagent dispensing position as shown inFIG. 3is defined as a first photometric point. Then, analysis data is generated based on ten pieces of test sample data generated by the photometric part13through measurement at the twentieth to twenty-ninth points, which are points that the reaction container3with the second mixture composed of the test sample, the first reagent and the second reagent passes through the photometric position for the twentieth to twenty-ninth times.

FIG. 11is a view showing an example of the measurement item selecting screen displayed on the display62. A measurement item selecting screen64includes: an “ID” field for displaying, for example, subject IDs of test samples set in the subject information setting screen; an “item” field for displaying the test item set in the analysis condition setting screen63ofFIG. 10; and a measurement item setting area64afor selecting an test item measured for each of the subject IDs displayed in the “ID” field from the “item” field.

In the “ID” field, for example, a subject ID “1” set in the subject information setting screen is displayed. Moreover, in the “item” field, for example, a test item name “AST” set in the analysis condition setting screen63is displayed.

In the measurement item setting area64a, “∘” is displayed in an area of the test item name selected in the “item” field corresponding to the subject ID selected in the “ID” field, whereas “.” is displayed in an unselected area. In a case where the test item name “AST” is selected for the sample ID “1” through the operation part70, “∘” equivalent to a first test is displayed in the area of “AST” of the “item” field corresponding to “1” of the “ID” field in the measurement item setting area64a.

Next, a series of operations by the automatic analyzing apparatus100according to the embodiment of the present invention will be described with reference toFIG. 12.FIG. 12is a flowchart showing the operation of the automatic analyzing apparatus100. In the memory circuit of the system controller80, analysis conditions set in the analysis condition setting screen63and information on a test item for each test sample selected in the measurement item selecting screen64are stored. Moreover, information on reagent properties such as viscosity and bubbliness of the first reagent and the second reagent set in advance for each test item is stored.

After the sample container17in which the test sample of the subject ID set in the subject information setting screen is installed in the disk sampler5of the analyzer18, when a measurement start operation for measuring the test sample is performed through the operation part70, the automatic analyzing apparatus100starts the operation (step S1).

The system controller80instructs measurement of the test sample to the controller42of the analysis controller40. The analyzer18is controlled by the controller42to measure the test item of the test sample selected and set in the measurement item selecting screen64, based on analysis conditions for each test item set in the analysis condition setting screen63. When the reaction container3cleaned and dried in the cleaning unit12stops at the distributing position, the stir piece moving part22of the stir part11in the analyzer18moves the stir piece20from the housing part21into the reaction container3(step S2).

When the reaction container3in which the stir piece20is housed stops at the sample dispensing position, the sample dispensing probe16dispenses the test sample corresponding to the test item of a first test from the sample container17into the reaction container3. When the reaction container3into which the test sample has been dispensed stops at the first reagent dispensing position, the first reagent dispensing probe14dispenses the first reagent for the test item of the first test from the reagent container6of the first reagent storage1into the reaction container3.

The reaction container3with the first reagent dispensed at the first reagent dispensing position rotationally moves in the R1direction.

The first driver25of the stirring part11vertically moves the stir piece20in the rotationally moving reaction container3and stirs the first mixture (step S3).

The second driver26vertically moves the stir piece20in the reaction container3stopping at the first stirring position after the rotational movement and stirs the first mixture (step S4).

The reaction container3with the first reagent dispensed repeats the rotational movement and the stoppage, and stops at the second reagent dispensing position after a specified time. The second reagent dispensing probe15dispenses the second reagent for the test item of the first test from the reagent container7of the second reagent storage2into the reaction container3stopping at the second reagent dispensing position. The reaction container3with the second reagent dispensed rotationally moves in the R1direction. The first driver27vertically moves the stir piece20in the rotationally moving reaction container3and stirs the second mixture (step S6).

The second driver28vertically moves the stir piece20in the reaction container3stopping at the second stirring position after rotationally moving and stirs the second mixture (step S6).

Based on the previously set test items and the analysis conditions such as the sample amount, the first reagent amount and the second reagent amount set in the analysis condition setting screen63, it is possible to vary the level of a direct current supplied to the driver23, the level of an alternate current, the frequency of the alternate current, the time to supply electric power and the rotation speed of the reaction container3. For example, in a case where the viscosity of the first reagent or the second reagent of the previously set test item is higher than normal viscosity, it is possible to supply an electric current larger than that for a reagent with the normal viscosity to the first and second drivers25,26or the first and second drivers27,28and cause the stir piece20to work with stronger force, and thereby more uniformly stir the first mixture or the second mixture in the reaction container3corresponding to the test item.

Further, for example, in a case where the first reagent or the second reagent of the previously set test item bubbles more easily than a normal reagent, it is possible to supply an electric current smaller than that for the normal reagent to the first and second drivers25,26or the first and second drivers27,28and cause the stir piece20to work with weaker force, and thereby suppress bubbling of the first mixture or the second mixture in the reaction container3corresponding to the test item.

Consequently, it is possible to prevent the second mixture from being insufficiently mixed when the second reagent is dispensed into the bubbling first reagent due to the bubbliness of the first mixture.

Moreover, it is possible to suppress bubbling up to the upper part in the reaction container3that the cleaning unit12cannot reach to clean.

After the second mixture is stirred, the photometric part13measures the second mixture in the reaction container3at the photometric point set in the analysis condition setting screen63that is a photometric position of the reaction container3. Then, the generated test sample data is outputted to the calculator51of the data processor50. The stir piece20when passing through the photometric position is sunk to the bottom inside the reaction container3that is not included in a region of a path of light emitted from the photometric part13.

The stir piece moving part22moves the stir piece20after measurement from the reaction container3into the stir piece cleaning part24(step S7).

The stir piece cleaning part24cleans the stir piece20after measurement moved by the stir piece moving part22(step S8).

The stir piece moving part22moves the stir piece20after cleaning from the stir piece cleaning part24into the housing part21(step S9).

The housing part21dries the cleaned stir piece20moved by the stir piece moving part22(step S10).

The calculator51reads out a previously created calibration curve from the data storage52. Next, the calculator51generates analysis data from test sample data outputted from the photometric part13by using the calibration curve having been read out, and stores the generated analysis data into the data storage52and also outputs it to the output part60.

The cleaning unit12absorbs the mixture after measurement in the reaction container3, and cleans and dries the inside of the reaction container3. At the point of time that the cleaning and drying of the reaction container3is finished and the analysis data of the test item of the sample ID “1” is outputted to the output part60, the automatic analyzing apparatus100ends the operation (step S11).

According to the embodiment of the present invention described above, during the rotation movement of the reaction container3into which the first reagent has been dispensed at the first reagent dispensing position and in which the stir piece20and the sample are housed, it is possible to vertically move the stir piece20in the reaction container3by the first driver25and stir the first mixture. Moreover, while the reaction container3with the first reagent dispensed is stopping after rotationally moving, it is possible to vertically move the stir piece20in the reaction container3by the second driver26and stir the first mixture. Consequently, it is possible to stir the first mixture in the reaction container3for a long time period during the rotation movement and the stoppage of the reaction container3.

Further, during the rotation movement of the reaction container3into which the second reagent has been dispensed at the second reagent dispensing position, it is possible to vertically move the stir piece20in the reaction container3by the first driver27and stir the second mixture. Moreover, during the stoppage of the reaction container3with the second reagent dispensed after the rotational movement, it is possible to vertically move the stir piece20in the reaction container3by the second driver28and stir the second reagent. Consequently, it is possible to stir the second mixture in the reaction container3for a long time period during the rotation movement and the stoppage of the reaction container3.

Furthermore, based on the previously set test items and the analysis conditions such as the sample amount, the first reagent amount and the second reagent amount set in the analysis condition setting screen63, it is possible to vary the level of a direct current supplied to the driver23, the level of an alternate current, the frequency of the alternate current, the time to supply, the rotation speed of the reaction container3, etc.

Accordingly, it becomes possible to increase the stir performance, whereby it is possible to prevent deterioration of analysis data due to insufficient stir.

The drivers25to28may be surrounded with a magnetic shield material having high magnetic permeability. By passing a magnetic line through the inside of the shield member, it becomes possible to decrease the influence of the magnetic line on the surroundings of the drivers.

Further, the stir piece may be driven by the action of the electric field. For example, the lower face of the stir piece is positively charged (plus) or negatively charged (minus). Then, the stir piece is driven upward and downward alternately by a driver disposed below the reaction container. The driver has an electrode having a plane formed so as to be capable of facing the reaction case, and the driver moves the stir piece relatively to the reaction container so that the electrode indicates either plus or minus to draw the stir piece downward and indicates the other to repulse the stir piece upward.