METHOD FOR MIXING IMMUNOASSAY REAGENT IN IMMUNOASSAY ANALYSIS DEVICE AND IMMUNOASSAY ANALYSIS DEVICE

A method for mixing immunoassay reagent in immunoassay analysis device and an immunoassay analysis device are provided. The method disperses aggregated magnetic beads in a reagent tray of the immunoassay analysis device by keeping an immunoassay reagent tube containing the immunoassay reagent in a variable speed state during rotation and/or by exerting an impact force on the immunoassay reagent tube during rotation of the immunoassay reagent tube, so as to keep the immunoassay reagent in a mixed state. The method can mix the immunoassay reagent, avoid the phenomenon of magnetic beads' aggregation and ensure the accuracy of the testing results.

CROSS-REFERENCE TO RELATED APPLICATION

This application claims the priority benefit of China application serial no. 202311013030.1, filed on Aug. 14, 2023. The entirety of the above-mentioned patent application is hereby incorporated by reference herein and made a part of this specification.

TECHNICAL FIELD

The present invention relates to a method for mixing a reagent and a device using same, and particularly to a method for mixing an immunoassay reagent in an immunoassay analysis device and an immunoassay analysis device, which belong to the technical field of medical testing.

BACKGROUND

It has been more than half a century since the first automatic chemical analysis instrument was manufactured, and the full automatic immunoassay analysis device has become technologically mature. The existing full automatic immunoassay analysis device has the following characteristics: 1. a multi-degree-of-freedom robotic arm is used to coordinate the actions of modules; 2. the device has strong flexibility and thus can meet various analysis requirements; 3. the test speed is high, and the time of continuous operation without intervention is long; and 4. the combination of multiple technologies and the full automation of processing are realized in testing technologies so that the testing results are more accurate and more precise. The full automatic immunoassay analysis device can realize the steps of taking a reaction cup, adding samples and reaction solutions, shaking up, promoting the reaction, measuring, performing operational analysis and cleaning in the experimental testing process, and substitute manual operation, which not only saves labor costs, but more importantly, eliminates human errors and ensures the accuracy of data. The full automatic immunoassay analysis device has the advantages of high speed, high efficiency, high precision and repeated consistency, has been widely used in the fields of processing, production, testing and living assistance, and is bound to become a trend in the field of medical testing.

The reagent required for the reaction is stored in a kit, and the kit is mounted on a reagent tray, so the reagent tray of the full automatic immunoassay analysis device is the storage and supply unit of the reagent required for the reaction. When the reagent is to be aspirated, the kit on the reagent tray is rotated by means of rotation of the reagent tray to the position where a reagent needle needs to aspirate the reagent, and then the reagent in the kit is aspirated into an incubation tray by the reagent needle to react with the sample.

As shown inFIG.1andFIG.2, a plurality of independent reagent chambers2are arranged in a kit1, the upper end of each reagent chamber2is opened to facilitate the reagent needle to aspirate the reagent, and each reagent chamber2contains a different reagent; and one end of the kit1is also rotationally connected with an immunoassay reagent tube3, the bottom of the immunoassay reagent tube3is provided with a reagent tube gear311, a plurality of kits1are successively installed on a reagent turntable4along the circumferential direction of the reagent turntable4, and the reagent turntable4can drive the plurality of kits1to rotate together. The immunoassay reagent tube3contains the immunoassay reagent, and the immunoassay reagent is prone to precipitation and accumulation due to the characteristics thereof and needs to keep rotating during the testing aspirating process to prevent precipitation and accumulation. Therefore, during the testing process, the prior art is that a toothed disc5is arranged in the central position of the reagent turntable4, the reagent tube gear311at the bottom of the immunoassay reagent tube3in each kit is engaged with the toothed disc5, the reagent turntable4is driven by a power mechanism to rotate, the toothed disc5in the central position is held still, and the plurality of kits1are driven by the reagent turntable4to rotate together, so as to drive the immunoassay reagent tubes3in the plurality of kits1on the reagent turntable4to keep rotating by engaged transmission of the toothed disc5and the reagent tube gear311to prevent the immunoassay reagent in the immunoassay reagent tubes3from precipitating and accumulating to ensure the accuracy of the testing results.

The prior art has the problem that although the immunoassay reagent tube is rotating most of the time, the mixing effect of the magnetic beads and the reagent solution in the immunoassay reagent is not very good, and the phenomenon of magnetic bead aggregation occurs, which affects the accuracy of the testing results.

After retrieval, the following patent documents are found:I. The Chinese invention patent application with the application publication No. of CN110160957A and the application publication date of Aug. 23, 2019 discloses a reagent tray module, comprising an open device and a rotating component arranged at the bottom of the open device and drives the open device to rotate, the side wall of the open device is provided with a scanning part, and a scanning device can recognize the properties of the reagent in the open device through the scanning part.II. The Chinese utility model patent with the authorized announcement No. of CN217156535U and the authorized announcement date of Aug. 9, 2022 discloses a sample and reagent integration device, comprising a base, and the base is rotationally provided with a reagent warehouse system and a sample system, wherein the reagent warehouse system has a circular structure, the sample system has an annular structure surrounding the reagent warehouse system, and the base is provided with a first driving component and a second driving component which are respectively used for driving the reagent warehouse system and the sample system to rotate.

The immunoassay reagent in the reagent tray in the above two patent documents will have the above problem.

To sum up, how to design a method for mixing an immunoassay reagent in an immunoassay analysis device and an immunoassay analysis device to mix the immunoassay reagent so as to avoid the phenomenon of magnetic bead aggregation and ensure the accuracy of the testing results is the primary technical problem to be solved urgently.

SUMMARY OF INVENTION

The primary technical problem to be solved by the present invention is to disclose a method for mixing an immunoassay reagent in an immunoassay analysis device and an immunoassay analysis device in view of the defects in the prior art, which can mix the immunoassay reagent, thus avoiding the phenomenon of magnetic bead aggregation and ensuring the accuracy of the testing results.

To solve the above technical problem, the present invention adopts the following technical solution: a method for mixing an immunoassay reagent in an immunoassay analysis device, which disperses aggregated magnetic beads in a reagent tray of an immunoassay analysis device by keeping an immunoassay reagent tube containing the immunoassay reagent in a variable speed state during rotation and/or by exerting an impact force on the immunoassay reagent tube during rotation of the immunoassay reagent tube, so as to keep the immunoassay reagent in a mixed state.

Preferably, continuous teeth distributed on a toothed disc in the reagent tray along a circumferential direction are designed into discontinuous teeth so that a plurality of tooth segments and notch segments are formed on the toothed disc, a kit is installed on a reagent turntable, the immunoassay reagent tube is rotationally connected to the kit, and the reagent turntable can rotate relative to the toothed disc, so as to drive a reagent tube gear at the bottom of the immunoassay reagent tube to move along the circumferential direction of the toothed disc.

In the working process, when the immunoassay reagent tube is located on the tooth segments on the toothed disc, the tooth segments are engaged with the reagent tube gear at the bottom of the immunoassay reagent tube to make the immunoassay reagent tube rotate; when the immunoassay reagent tube is located on the notch segments on the toothed disc, the immunoassay reagent tube loses power of rotation so that the rotation speed of the immunoassay reagent tube in the notch segments is changed once; and when the immunoassay reagent tube is located on the tooth segments on the toothed disc again, the tooth segments can be engaged with the reagent tube gear at the bottom of the immunoassay reagent tube again, and the speed of the immunoassay reagent tube is changed again to return to the rotation state, so repeatedly, until the rotation speed of the immunoassay reagent tube is repeatedly in a variable state in the working process.

When the immunoassay reagent tube is moved from the notch segments to the tooth segments on the toothed disc, the tooth segments that are just engaged with the reagent tube gear will form an impact force on the immunoassay reagent tube, which plays an impact role.

Preferably, the arc length of the tooth segments is set to L, and the arc length of the notch segments is L/2.

Preferably, the inside wall of the immunoassay reagent tube is provided with tube bumps; and the immunoassay reagent tube can further mix the immunoassay reagent by the tube bumps during rotation at a variable speed and under the action of the impact force.

Preferably, the number of the tube bumps is set to two, and the two tube bumps are distributed symmetrically about the central axis of the immunoassay reagent tube.

Preferably, in the working process, when it is not necessary to aspirate the reagent, the reagent turntable is held still by controlling the rotation of the toothed disc so that the immunoassay reagent tube rotates.

When it is necessary to aspirate various reagents in the kit, the reagent turntable is controlled to drive the kit to rotate to a reagent aspirating point A, the kit at point A is held still, and then various reagents in the kit are aspirated.

Preferably, the rotation direction of the toothed disc is controlled to be opposite to that of the reagent turntable.

The present invention also discloses an immunoassay analysis device, comprising a reagent tray and a cleaning tray arranged on one side of the reagent tray, wherein the reagent tray adopts the above method for mixing an immunoassay reagent to mix the immunoassay reagent. Preferably, the cleaning tray comprises a cleaning tray barrel body, a turntable

mechanism arranged in the cleaning tray barrel body and a needle body lifting mechanism arranged above the cleaning tray barrel body, injection needles and aspirating needles are arranged on the needle body lifting mechanism, reaction cups are placed on a turntable of the turntable mechanism, the reaction cups can be driven through the turntable to rotate, a cleaning tank is arranged in the cleaning tray barrel body and below the turntable, and the injection needles and the aspirating needles can be driven by the downward movement of the needle body lifting mechanism to move down to be inserted into the reaction cups to clean the reaction cups and to move down to be inserted into the cleaning tank to be cleaned after the reaction cups are removed.

Preferably, the cleaning tank comprises a fully enclosed annular tank body, and injection needle cleaning barrels and aspirating needle cleaning barrels which are arranged on the annular tank body, wherein the inner space of the injection needle cleaning barrels and the aspirating needle cleaning barrels is communicated with the inner space of the annular tank body, the bottom surface of the annular tank body is also provided with a drain pipe for draining water, and the top surfaces of each injection needle cleaning barrel and each aspirating needle cleaning barrel are respectively provided with openings for the injection needles and the aspirating needles to insert;

and the injection needle cleaning barrels and the aspirating needle cleaning barrels are arranged according to the positions of the injection needles and the aspirating needles so that one injection needle is cleaned by one injection needle cleaning barrel and one aspirating needle is cleaned by one aspirating needle cleaning barrel.

Preferably, each aspirating needle cleaning barrel is vertically penetrated through the annular tank body, each aspirating needle cleaning barrel comprises an outer barrel body and an inner barrel body arranged in the outer barrel body, an annular space is formed between the outer barrel body and the inner barrel body, the annular space is communicated with the inner space of the annular tank body, the top surface of the inner barrel body is lower than that of the outer barrel body, the opening of each aspirating needle cleaning barrel is arranged on the top surface of the outer barrel body, the aspirating needle cleaning barrel arranged below the annular tank body is also provided with a water inlet, the water inlet is communicated with the bottom of the inner cavity of the inner barrel body, and the top of the inner cavity of the inner barrel body is communicated with the inner space of the outer barrel body.

Preferably, each injection needle cleaning barrel comprises a barrel body, the bottom end of the barrel body is arranged at the top of the annular tank body, the inner cavity of the barrel body is communicated with the inner space of the annular tank body, and the opening of each injection needle cleaning barrel is arranged on the top end of the barrel body.

Preferably, a reaction cup vibrating mechanism is arranged in the cleaning tray barrel body, the reaction cup vibrating mechanism comprises a guide rail I and a guide rail II which are arranged in the cleaning tray barrel body and an impactor which is slidably connected to the guide rail I and the guide rail II, an impactor driving mechanism is also arranged in the cleaning tray barrel body, and the impactor driving mechanism is used to drive a push block to move back and forth along the guide rail I and the guide rail II so as to use the impactor to repeatedly impact the reaction cups on the turntable located at an injection station so that the magnetic beads in the reaction cups are dispersed by the impact.

Preferably, the impactor driving mechanism comprises an impactor driving motor arranged in the cleaning tray barrel body and an impactor rotating shaft I rotationally connected in the cleaning tray barrel body, the output shaft of the impactor driving motor is in matched rotational connection with one end of the impactor rotating shaft I, the end surface of the other end of the impactor rotating shaft I is also provided with an impactor rotating shaft II, the central axis of the impactor rotating shaft I does not coincide with that of the impactor rotating shaft II, the impactor is provided with a slotted hole, the impactor rotating shaft II is inserted into the slotted hole for matched sliding connection so as to control the impactor driving motor to drive the impactor rotating shaft I and the impactor rotating shaft II to rotate, and the impactor can be driven by the contact between the impactor rotating shaft II and the inner circumferential surface of the slotted hole to move back and forth along the guide rail I and the guild rail II to repeatedly impact the reaction cups.

Preferably, three basic cleaning units are successively arranged on the needle body lifting mechanism along the circumferential direction of the turntable, the basic cleaning units comprise two basic cleaning units I and one basic cleaning unit II, an injection needle I and an aspirating needle are arranged in each basic cleaning unit I, an integrated double injection needle and an aspirating needle are arranged in the basic cleaning unit II, and the integrated double injection needle comprises an injection needle I and an injection needle II; and along the circumferential direction of the turntable, needle bodies are distributed on the needle body lifting mechanism in the following order: injection needle I, aspirating needle, injection needle I, aspirating needle, integrated double injection needle and aspirating needle.

Preferably, the integrated double injection needle is connected to a lifting plate of the needle body lifting mechanism through a limiting block, the limiting block comprises a base arranged on the lifting plate and a screw cap threaded to the base, the base comprises a bottom plate and a cylinder body arranged on the bottom plate, the cylinder body and the bottom plate are connected through a mounting through hole, the inner circumferential surface of the cylinder body penetrated by the mounting through hole is provided with a groove, the groove is concave along the radial direction of the cylinder body, one side of the groove is open, and the other three sides are closed.

The bottom of the screw cap is provided with a screw cap through hole, the integrated double injection needle is also provided with a guide block which is cylindrical, the diameter of the guide block is matched with that of the mounting through hole, and one side of the guide block is provided with a bump protruding radially; and during installation, the injection needle I and the injection needle II are successively penetrated through the screw cap through hole of the screw cap, the guide block and the mounting through hole of the base so that the bump of the guide block is stuck into the groove, the groove is matched with the bump to limit the injection needle I and the injection needle II in the circumferential direction, the screw cap is tightened onto the cylinder body, and the bump is pressed into the groove by the screw cap to limit the injection needle I and the injection needle II in the axial direction.

The present invention has the following beneficial effects: the present invention can mix the immunoassay reagent, thus avoiding the phenomenon of magnetic bead aggregation and ensuring the accuracy of the testing results. The reagent tube gear at the bottom of the immunoassay reagent tube can be driven to move along the circumferential direction of the toothed disc by designing the toothed disc to have discontinuous teeth and rotating the reagent turntable relative to the toothed disc, so as to realize the technical solution of dispersing aggregated magnetic beads by changing the state of uniform rotation during rotation of the immunoassay reagent tube and exerting an impact force during rotation of the immunoassay reagent tube. The reagent turntable is held still by controlling the rotation of the toothed disc so that the immunoassay reagent tube achieves the effect of active mixing. In this way, the entire testing time is greatly shortened compared with the previous mixing methods, thus improving the testing efficiency. By designing the cleaning tray in the immunoassay analysis device, the cleaning tank is integrated in the cleaning tray and located below the turntable, making full use of the inner space of the cleaning tray and the moving stroke of the needle body lifting mechanism. During normal operation of the cleaning tray, the needle body lifting mechanism is moved down so that the injection needles and the aspirating needles are moved down to the working station to clean the reaction cups. When the injection needles and the aspirating needles need to be cleaned, the reaction cups are removed, and then the needle body lifting mechanism continues to move down so that the injection needles and the aspirating needles are moved down beyond the working station until inserted into the cleaning tank to clean the needle bodies. Therefore, the present invention can not only ensure that the injection needles and the aspirating needles can normally clean the magnetic beads in the reaction cups, but also clean the injection needles and the aspirating needles, which simplifies the cleaning steps, improves the convenience of cleaning the injection needles and the aspirating needles and improves the cleaning effect of the injection needles and the aspirating needles. Through the design of the cleaning tank and the specific design of the cleaning steps, the inner and outer walls of the aspirating needles and the inner walls of the injection needles are cleaned at the same time, and the cleaning effect is improved. The reaction cup vibrating mechanism is arranged in the cleaning tray. When the injection needles inject solutions into the reaction cups, the reaction cup vibrating mechanism is used to repeatedly impact the reaction cups so that the magnetic beads in the reaction cups are dispersed by the repeated impact, and the magnetic beads are cleaned with the cleaning solutions injected by the injection needles, thus ensuring the cleaning effect. The reaction cup vibrating mechanism is arranged in the cleaning tray. When the injection needles inject solutions into the reaction cups, the reaction cup vibrating mechanism is used to repeatedly impact the reaction cups so that the magnetic beads in the reaction cups are dispersed by the repeated impact, and the magnetic beads are cleaned with the cleaning solutions injected by the injection needles, thus ensuring the cleaning effect. With a double injection needle mechanism, the present invention can use two different cleaning solutions to cooperate with each other, which can not only achieve the effect of cleaning the magnetic beads in the reaction cups, but also remove the residual bubbles in the reaction cups, thus ensuring the accuracy of the final testing results.

DESCRIPTION OF EMBODIMENTS

The technical solution of the present invention is further described in detail below in combination with the drawings and specific embodiments.

Although the immunoassay reagent tube is rotated most of the time, the mixing effect of the magnetic beads and the reagent solution in the immunoassay reagent is not very good, and the phenomenon of magnetic bead aggregation occurs. The applicant finds through research that the main reason is that the immunoassay reagent tube is rotated most of the time, but is always in a state of slower uniform rotation, and the magnetic beads are very easy to be thrown to the inside wall of the immunoassay reagent tube under the action of a centrifugal force, resulting in the phenomenon of magnetic bead aggregation.

The present invention discloses a method for mixing an immunoassay reagent in an immunoassay analysis device, which disperses aggregated magnetic beads by keeping an immunoassay reagent tube in a variable speed state during rotation and/or by exerting an impact force on the immunoassay reagent tube during rotation of the immunoassay reagent tube, so as to keep the immunoassay reagent in a mixed state. The applicant finds through research that the above problem can be solved by changing the state of uniform rotation during rotation of the immunoassay reagent tube, i.e., the immunoassay reagent tube is not kept in a state of uniform rotation, and the rotation speed is changed so that the centrifugal force on the immunoassay reagent tube will also be changed, thus changing the state of magnetic bead aggregation; or the aggregated magnetic beads can be dispersed by exerting an external impact force during rotation of the immunoassay reagent tube. Most preferentially, the phenomenon of magnetic bead aggregation is eliminated by the above two technical solutions. The applicant finds through tests that such technical solutions can achieve the best effect, and no phenomenon of magnetic bead aggregation occurs in the immunoassay reagent simultaneously mixed by the two technical solutions.

Embodiment: the technical solution of dispersing aggregated magnetic beads by changing the state of uniform rotation during rotation of the immunoassay reagent tube and exerting an impact force during rotation of the immunoassay reagent tube is shown inFIG.3, and the applicant designs continuous teeth distributed on the toothed disc5along a circumferential direction into discontinuous teeth to form notches between the adjacent tooth segments. In the present embodiment, the toothed disc5is provided with ten tooth segments511and ten notch segments512. In the working process, when the immunoassay reagent tube is located on the tooth segments511on the toothed disc5, the tooth segments511are engaged with the reagent tube gear311at the bottom of the immunoassay reagent tube to provide a rotational force to the immunoassay reagent tube to make the immunoassay reagent tube rotate; when the immunoassay reagent tube is located on the notch segments512on the toothed disc5, since no teeth in the notch segments512are engaged with the reagent tube gear311, the immunoassay reagent tube loses power of rotation, and since the rotation speed is not high, the immunoassay reagent tube slows down and sometimes even stops rotating after losing power of rotation, so the rotation speed of the immunoassay reagent tube in the notch segments512is changed once; and when the immunoassay reagent tube is located on the tooth segments511on the toothed disc5again, the tooth segments511can be engaged with the reagent tube gear311at the bottom of the immunoassay reagent tube again, a rotational force is provided to the immunoassay reagent tube, and the speed of the immunoassay reagent tube is changed again to return to the rotation state after obtaining the force, so repeatedly, until the rotation speed of the immunoassay reagent tube is repeatedly in a variable state in the working process. In addition, when the immunoassay reagent tube is moved from the notch segments512to the tooth segments511on the toothed disc, the tooth segments511that are just engaged with the reagent tube gear311will form an impact force on the immunoassay reagent tube and play an impact role. The immunoassay reagent tube disperses the aggregated magnetic beads therein under the double actions of the above rotational variable speed state and the impact force, so as to keep the immunoassay reagent in a mixed state. The present embodiment can mix the immunoassay reagent, thus avoiding the phenomenon of magnetic bead aggregation and ensuring the accuracy of the testing results.

The applicant finds through many tests that the arc length of the tooth segments511is set to L and the arc length of the notch segments512is L/2, which can achieve the best mixing effect of the immunoassay reagent in the immunoassay reagent tube.

To further improve the mixing effect, the inside wall of the immunoassay reagent tube3is provided with tube bumps312, as shown inFIG.4. The immunoassay reagent tube3can further mix the immunoassay reagent by the tube bumps312during rotation at a variable speed and under the action of the impact force, which can further improve the mixing effect. In the present embodiment, the number of the tube bumps312is set to two, and the two tube bumps312are distributed symmetrically about the central axis of the immunoassay reagent tube3.

The prior art has another problem. As shown inFIG.5, in the prior art, the toothed disc5in the central position is held still in the working process, and the reagent turntable4is controlled to drive the kit1to rotate so that the immunoassay reagent tube3on the kit1rotates through engagement of the toothed disc5and the reagent tube gear311. Generally, reagents contained in the immunoassay reagent tube3and a plurality of reagent chambers2on the kit1are used for testing of the same index. When an index is tested, a reagent aspirating point is assumed to be point A. When the immunoassay reagent in the immunoassay reagent tube3on the kit1is to be aspirated, the reagent turntable4is controlled first to drive the kit1to rotate to point A, and then the immunoassay reagent in the immunoassay reagent tube3is aspirated. After aspiration, the kit1shall not be stopped here, but the reagent turntable4shall be controlled to drive the kit1to continue to turn a circle to reach point A again, and then the reagent contained in one reagent chamber2on the kit1is aspirated. After aspiration, the reagent turntable4is controlled to drive the kit1to continue to turn a circle to reach point A again, and the reagent contained in the next reagent chamber2on the kit1is aspirated, so repeatedly, until the reagents contained in the immunoassay reagent tube3and the plurality of reagent chambers2on the kit1are aspirated, thereby completing the test of the index. Such mixing method will lead to an increase in the entire testing time, thus reducing the testing efficiency.

Therefore, as shown inFIG.6, the applicant arranges a driving mechanism I and a driving mechanism II on the toothed disc5and the reagent turntable4respectively. In the working process, the toothed disc5is driven by the driving mechanism I to rotate, and the reagent turntable4is held still so that the kit1on the reagent turntable4is also stationary, and the immunoassay reagent tube3on the kit1rotates only by engagement of the toothed disc5and the reagent tube gear311. When it is necessary to aspirate various reagents in the kit1, the reagent turntable4is driven by the driving mechanism II to drive the kit1to rotate to the reagent aspirating point A, and then the kit1at point A is held still. At this time, the toothed disc5is still rotated, so the immunoassay reagent tube3on the kit1is still in a state of rotation. Therefore, after the immunoassay reagent in the immunoassay reagent tube3on the kit1is aspirated, the kit1can still be fixed at point A, so as to continue to aspirate reagents in several other reagent chambers2on the kit1. In this way, compared with the previous mixing methods, the entire testing time is greatly shortened, thus improving the testing efficiency.

In the present embodiment, the rotation direction of the toothed disc5is controlled to be opposite to that of the reagent turntable4so that the toothed disc5can be in a state of continuous rotation, and the reagent turntable4, whether rotating or not, can cause the immunoassay reagent tube3of the kit1on the reagent turntable4to keep rotating, avoiding precipitation and accumulation of the immunoassay reagent in the immunoassay reagent tube3.

As shown inFIG.7andFIG.8, the reagent turntable4is installed in the reagent tray6of the immunoassay analysis device, the reagent tray6comprises a reagent tray barrel body611and a driving mechanism I arranged at the bottom of the reagent tray barrel body611, the reagent turntable4is located in the reagent tray barrel body611, the driving mechanism I is a driving motor I7, the output shaft of the driving motor I7is connected with the reagent turntable4, and the reagent turntable4can be driven by the action of the driving motor I7to rotate; and the bottom of the reagent turntable4is also provided with casters8, and the casters8are in contact with the inner bottom of the reagent tray barrel body611, so as to ensure that the reagent turntable4rotates more smoothly. The toothed disc5is rotationally connected in the reagent tray barrel body611and below the reagent turntable4, the toothed disc5and the reagent turntable4are coaxial, the bottom of the reagent tray barrel body611is also provided with a driving mechanism II, the driving mechanism II is a driving motor II9, the output shaft of the driving motor II9is in matched transmission connection with the toothed disc5, and the toothed disc5can be driven by the action of the driving motor II9to rotate. The top of the reagent tray barrel body611is provided with a plurality of reagent through holes612for aspirating reagents, and the position of the reagent through holes612is the reagent aspirating point A.

As shown inFIG.9, the inner bottom of the reagent tray barrel body611is provided with a lug boss10, the inner ring of the toothed disc bearing11is sleeved on the outer circumferential surface of the lug boss10, the toothed disc5is connected with the outer ring of the toothed disc bearing11, the outer ring of the toothed disc bearing11is sleeved with a driving toothed disc I12, the output shaft of the driving motor II9is also provided with a driving toothed disc II13, and the driving toothed disc I12is in engaged transmission connection with the driving toothed disc II13so that the toothed disc5can be driven under the action of the driving motor II9to rotate. The reagent tube gear311of the immunoassay reagent tube on the kit1installed on the reagent turntable4is in engaged transmission connection with the toothed disc5.

As shown inFIG.10, the present invention also discloses an immunoassay analysis device, comprising a sample transfer channel14, and the reagent tray6, the incubation tray15, the cleaning tray16, an optical testing mechanism17and a consumables warehouse18which are arranged on one side of the sample transfer channel14, a cup grabbing swing arm19is also arranged between the incubation tray15and the cleaning tray16, the cup grabbing swing arm19is used for switching and grabbing the reaction cup back and forth between the incubation tray15and the cleaning tray16, a reagent needle swing arm20is also arranged between the incubation tray15and the reagent tray6, the reagent needle swing arm20is used for aspirating and adding the reagent from the reagent tray6into the reaction cup of the incubation tray15, and a reagent needle cleaning pool21is arranged beside the reagent needle swing arm20to clean the reagent needle swing arm20after the reagent needle swing arm20aspirates a reagent so that the reagent needle swing arm20aspirates another reagent. A test chip module181, a tip module182and a reaction cup module183are arranged in the consumables warehouse18, and a plurality of components are stored in each module, i.e., a plurality of test chips are placed in the test chip module181, a plurality of tips are placed in the tip module182, and a plurality of reaction cups are placed in the reaction cup module183so that the immunoassay analysis device can perform multiple tests. A three-dimensional movable robotic arm60is also arranged among the incubation tray15, the optical testing mechanism17and the consumables warehouse18, and the three-dimensional movable robotic arm60is used for replacing consumables and aspirating samples to be tested in the reaction cup of the incubation tray15to the optical testing mechanism17for testing.

As shown inFIG.11andFIG.12, the cleaning tray comprises a cleaning tray barrel body22, a turntable mechanism23arranged in the cleaning tray barrel body22and a needle body lifting mechanism24arranged above the cleaning tray barrel body22; and the reaction cup25is placed on a turntable231of the turntable mechanism23, and the reaction cup25can be driven by the turntable231to rotate. A cleaning tank26is arranged in the cleaning tray barrel body22and below the turntable231, and the needle body lifting mechanism24is provided with injection needles27and aspirating needles28. The method for cleaning the injection needles and the aspirating needles in the cleaning tray of the immunoassay analysis device is that during normal operation, the injection needles and the aspirating needles can be driven by the downward movement of the needle body lifting mechanism24to move down to be inserted into the reaction cups to clean the reaction cups, when the injection needles and the aspirating needles need to be cleaned, the reaction cups25on the turntable231are removed first, and the downward movement of the needle body lifting mechanism24is controlled so that the injection needles and the aspirating needles are inserted into the cleaning tank26by penetrating the turntable for cleaning. In the present embodiment, the cleaning tank is integrated in the cleaning tray and located below the turntable, making full use of the inner space of the cleaning tray and the moving stroke of the needle body lifting mechanism. During normal operation of the cleaning tray, the needle body lifting mechanism is moved down so that the injection needles and the aspirating needles are moved down to the working station to clean the reaction cups. When the injection needles and the aspirating needles need to be cleaned, the reaction cups are removed, and then the needle body lifting mechanism continues to move down so that the injection needles and the aspirating needles are moved down beyond the working station until inserted into the cleaning tank to clean the needle bodies. Therefore, the present embodiment can not only ensure that the injection needles and the aspirating needles can normally clean the magnetic beads in the reaction cups, but also clean the injection needles and the aspirating needles, which simplifies the cleaning steps, improves the convenience of cleaning the injection needles and the aspirating needles and improves the cleaning effect of the injection needles and the aspirating needles.

As shown inFIG.13, two basic cleaning units I and one basic cleaning unit II are successively arranged on the needle body lifting mechanism24along the circumferential direction of the turntable231, an injection station and an aspirating station are arranged in each basic cleaning unit, an injection needle I271and an aspirating needle28are respectively arranged at the injection station and the aspirating station of each basic cleaning unit I, an integrated double injection needle S and an aspirating needle28are respectively arranged at the injection station and the aspirating station of the basic cleaning unit II, and the integrated double injection needle S comprises an injection needle I271and an injection needle II272. Along the circumferential direction of the turntable231, the needle bodies are distributed on the needle body lifting mechanism24in the following order: injection needle I271, aspirating needle28, injection needle I271, aspirating needle28, integrated double injection needle S and aspirating needle28.

Since the injection needles and the aspirating needles are distributed along the circumferential direction of the turntable, as shown inFIG.14andFIG.15, the cleaning tank26is also set to have an annular shape, the cleaning tank26comprises a fully enclosed annular tank body261, and injection needle cleaning barrels262and aspirating needle cleaning barrels263which are arranged on the annular tank body261, wherein the inner space of the injection needle cleaning barrels912and the inner space of the aspirating needle cleaning barrels263are communicated with the inner space of the annular tank body261, the bottom surface of the annular tank body261is also provided with a drain pipe264for draining water, and the top surfaces of each injection needle cleaning barrel912and each aspirating needle cleaning barrel263are respectively provided with openings29for the injection needles and the aspirating needles to insert. The injection needle cleaning barrels262and the aspirating needle cleaning barrels263are arranged according to the distribution positions of the needle bodies on the needle body lifting mechanism24so that one injection needle is cleaned by one injection needle cleaning barrel262and one aspirating needle is cleaned by one aspirating needle cleaning barrel263. Therefore, the order of the cleaning barrels on the annular tank body in the present embodiment is as follows: injection needle cleaning barrel262, aspirating needle cleaning barrel263, injection needle cleaning barrel262, aspirating needle cleaning barrel263, injection needle cleaning barrel262and aspirating needle cleaning barrel263. With the design of the annular tank body and the annularly distributed cleaning barrels, the present embodiment can clean the injection needles and the aspirating needles at the same time, which further improves the cleaning efficiency.

To further improve the cleaning effect, the applicant finds through research that as shown inFIG.16andFIG.17, in daily work, in the process of cleaning the magnetic beads in the reaction cups25on the cleaning tray, when the injection needles27are used to inject a solution into the reaction cups25, the injection needles27are inserted into the solution30in the reaction cups25, so it is only necessary to clean the inner walls of the injection needles27when cleaning the injection needles27; and when the aspirating needles28are used to aspirate the solution from the reaction cups25, the aspirating needles28shall be inserted into the solution30in the reaction cups25, so it is necessary to clean the inner walls and the outer walls of the aspirating needles28when cleaning the aspirating needles28.

Therefore, as shown inFIG.18andFIG.19, each aspirating needle cleaning barrel263is vertically penetrated through the annular tank body261, each aspirating needle cleaning barrel263comprises an outer barrel body2631and an inner barrel body2632arranged in the outer barrel body2631, an annular space2633is formed between the outer barrel body2631and the inner barrel body2632, the annular space2633is communicated with the inner space2611of the annular tank body261, the top surface of the inner barrel body2632is lower than that of the outer barrel body2631, the opening29of the aspirating needle cleaning barrel263is arranged on the top surface of the outer barrel body2631, the aspirating needle cleaning barrel263arranged below the annular tank body261is also provided with a water inlet31, the water inlet31is communicated with the bottom of the inner cavity26321of the inner barrel body2632, and the top of the inner cavity26321of the inner barrel body2632is communicated with the inner space of the outer barrel body2631.

When the aspirating needles28are cleaned, the aspirating needles28are controlled to be inserted into the inner cavity26321of the inner barrel body2632through the opening29at the top of the aspirating needle cleaning barrel263, an external water pump is used to transfer cleaning water to the inner cavity26321of the inner barrel body2632from the water inlet31, and the aspirating needles28are controlled to start inspiration so that a part of cleaning water is aspirated by the aspirating needles28, so as to clean the inner walls of the aspirating needles28with the cleaning water. Meanwhile, since the water supply of the water pump is greater than the water absorption of the aspirating needles28, most of the cleaning water is finally drained through the drain pipe264after flowing through the inner cavity26321of the inner barrel body2632, the annular space2633and the inner space2611of the annular tank body261so that the cleaning water flowing through the inner cavity26321of the inner barrel body2632is used to clean the outer walls of the aspirating needles28. With the above method, the inner walls and the outer walls of the aspirating needles28are cleaned simultaneously, thus improving the cleaning effect.

The water inlet31is arranged on the side of the aspirating needle cleaning barrel263so that when the aspirating needles are cleaned, the cleaning water enters the inner cavity26321of the inner barrel body2632from the side positions of the aspirating needles28. In this way, the cleaning water is not directly flushed into the aspirating needles from the bottoms of the aspirating needles, but is aspirated into the aspirating needles, ensuring the cleaning effect.

As shown inFIG.20andFIG.21, each injection needle cleaning barrel262comprises a barrel body2621, the bottom end of the barrel body2621is arranged at the top of the annular tank body261, the inner cavity of the barrel body2621is communicated with the inner space2611of the annular tank body261, and the opening29of the injection needle cleaning barrel262is arranged on the top end of the barrel body2621.

When the injection needles27are cleaned, the injection needles27are controlled to be inserted into the inner cavity of the barrel body2621through the opening29at the top of the barrel body2621, cleaning water is injected by the injection needles27, and the cleaning water is injected into the inner space2611of the annular tank body261and finally drained through the drain pipe264, so as to clean the inner walls of the injection needles27with the cleaning water flowing through the injection needles27, improving the cleaning effect.

As shown inFIG.12, a cleaning tray partition plate221is arranged in the cleaning tray barrel body22, and the interior of the cleaning tray barrel body22is divided into an upper barrel body space222located in an upper position and a lower barrel body space223located in a lower position by the cleaning tray partition plate221. The turntable231of the turntable mechanism23is located in the upper barrel body space222, an insulation material and a heating device (not shown in the figure) are arranged in the upper barrel body space222, and the heating device is an electric heating film. The interior of the upper barrel body space222is heated and maintained at a certain temperature by the insulation material and the heating device, because the reaction cups need to be maintained at a certain temperature after being placed on the turntable. The present embodiment can ensure that the reaction cups on the turntable are always in an environment with a certain operating temperature by arranging the turntable in the heat preservation space of the upper barrel body space.

As shown inFIG.13,FIG.22andFIG.23, the turntable mechanism23also comprises a turntable motor and a reducer232, the turntable motor and the reducer232are arranged on the cleaning tray partition plate221, and the rotating shaft2321of the turntable motor and reducer232extends into the upper barrel body space222to be connected with the turntable231. In the prior art, the turntable is driven by belt transmission. In this case, the transmission mechanism will be complex and occupy a large space. The present embodiment eliminates the belt transmission mechanism by directly connecting the turntable motor integrated with the reducer to the turntable, so as to simplify the structure of the transmission mechanism and reduce space usage.

A plurality of cup holes32for placing reaction cups are arranged on the turntable231along the circumferential direction, the top of the cleaning tray barrel body22is provided with a reaction cup through hole33, the reaction cup through hole33is used for picking and placing the reaction cups25, and the reaction cup through hole33is at the zero station. When one cup hole32in the turntable231is rotated to coincide with the central axis of the reaction cup through hole33, one reaction cup25is placed in the cup hole32through the reaction cup through hole33, or the reaction cup25in the cup hole32is removed through the reaction cup through hole33. Each cup hole32corresponds to one cleaning barrel. When the injection needles and the aspirating needles are cleaned, after the reaction cup25is removed from the cup hole32, and the injection needles and the aspirating needles are moved down to be inserted into the cleaning tank26by passing through the cup hole32in the turntable for cleaning.

As shown inFIG.11, the side of the cleaning tray barrel body22is also provided with a sensor I34, and the sensor I34is used to detect the number of reaction cups on the turntable and the presence or absence of reaction cups on the turntable. As shown inFIG.24, the bottom of the turntable231is provided with an induction ring35, the induction ring35is provided with a plurality of induction channels351, a sensor II36is also arranged on the cleaning tray partition plate221and at the induction ring35, and when the turntable is rotated, the induction channels351on the induction ring35are induced by the sensor II36to control the rotation angle and position of the turntable.

As shown inFIG.25, the step of injecting a solution into the reaction cups and the step of aspirating the solution from the reaction cups are set to be performed in one basic cleaning unit, the basic cleaning unit comprises the basic cleaning unit I37and the basic cleaning unit II38, the basic cleaning unit II38is a basic cleaning unit before testing, and the basic cleaning unit I37is another basic cleaning unit. The basic cleaning unit I37and the basic cleaning unit II38are successively distributed above the turntable231along the circumferential direction of the turntable231.

As shown inFIG.22andFIG.26, a magnet retaining ring39is arranged on the inner circumferential surface of the cleaning tray barrel body22and at the turntable231. A magnet is arranged on the magnet retaining ring39so that magnetic fields40are formed in the cleaning tray barrel body22and between the injection station and the aspirating station in each basic cleaning unit, the interior of the cleaning tray barrel body22at the injection station is located outside the magnetic field40, and the interior of the cleaning tray barrel body22at the aspirating station is located inside the magnetic field40. After the cleaning solution is injected into the reaction cups at the injection station, the reaction cups enter the magnetic fields40with the rotation of the turntable, and the magnetic fields40are used to adsorb the magnetic beads in the reaction cups until rotating to the aspirating station. When the solution is aspirated at the aspirating station, the magnetic fields40are used to adsorb the magnetic beads in the reaction cups, which can prevent the magnetic beads from being aspirated by the aspirating needles. After aspiration, the reaction cups are removed from the magnetic fields40to the injection station where no magnetic field exists, and then the injection needles are used to inject the solution into the reaction cups to impact the magnetic beads, so as to achieve the purpose of cleaning. In the present embodiment, a total of three magnetic fields40are formed, the magnet can be a permanent magnet or an electromagnet.

As shown inFIG.27andFIG.28, the cleaning tray of the present embodiment comprises a reaction cup vibrating mechanism41, and the reaction cup vibrating mechanism41is arranged on the cleaning tray partition plate221and also located in the upper barrel body space222. The reaction cup vibrating mechanism41comprises a guide rail I411and a guide rail II412which are arranged on the cleaning tray partition plate221and an impactor413which is in matched sliding connection to the guide rail I411and the guide rail II412through a sliding block, an impactor driving mechanism is also arranged on the cleaning tray partition plate221, and the impactor driving mechanism is used to drive a push block to move back and forth along the guide rail I411and the guide rail II412so as to use the impactor413to repeatedly impact the side of the reaction cups in the cup holes32in the turntable located at the injection station so that the magnetic beads in the reaction cups are dispersed by the impact. When the cleaning solution is aspirated from the reaction cups by the aspirating needles and transferred to the injection needles, the magnetic beads are adsorbed together. In the present embodiment, when the injection needles inject the solution into the reaction cups, the reaction cup vibrating mechanism is used to repeatedly impact the reaction cups so that the magnetic beads in the reaction cups are dispersed by the repeated impact, and the magnetic beads are cleaned with the cleaning solution injected by the injection needles, thus ensuring the cleaning effect.

As shown inFIG.28andFIG.29, the impactor driving mechanism comprises an impactor driving motor (not shown in the figures) arranged at the bottom of the cleaning tray partition plate221and an impactor rotating shaft I42rotationally connected to the top of the cleaning tray partition plate221, the output shaft of the impactor driving motor is in matched rotational connection with one end of the impactor rotating shaft I42, the end surface of the other end of the impactor rotating shaft I42is also provided with an impactor rotating shaft II43, and the central axis of the impactor rotating shaft I42does not coincide with that of the impactor rotating shaft II43, i.e., the central axis of the impactor rotating shaft II43is in a position that deviates from the central axis of the impactor rotating shaft I42. The impactor413is provided with a slotted hole44, the impactor rotating shaft II43is inserted into the slotted hole44for matched sliding connection so as to control the impactor driving motor to drive the impactor rotating shaft I42and the impactor rotating shaft II43to rotate, and the impactor413can be driven by the contact between the impactor rotating shaft II43and the inner circumferential surface of the slotted hole44to move back and forth along the guide rail I411and the guild rail II412to repeatedly impact the reaction cups.

As shown inFIG.30, the impactor rotating shaft II43comprises an impact bearing431and a lock bolt432, and the impact bearing431is locked and fixed on the end surface of the impactor rotating shaft I42by the lock bolt432by passing through the inner ring of the impact bearing431. When the impactor rotating shaft II43is inserted into the slotted hole44, the outer ring of the impact bearing431is in contact with the inner circumferential surface of the slotted hole44for matched sliding connection. Such design can further ensure the smoothness of the impactor during repeated movement. The outer circumferential surface of the impactor rotating shaft I42is provided with an induction disk45, a sensor III46is arranged on the cleaning tray partition plate221and at the induction disk45, and the state of the impactor rotating shaft I42can be tested by matching the induction disk45and the sensor III46.

As shown inFIG.25andFIG.28, the end of the impactor413is set to have an arc shape, and the arc end4131is used to impact the sides of the reaction cups25so that the reaction cups will not be damaged by the impact.

The reaction cup vibrating mechanism is arranged between the basic cleaning unit II38and the basic cleaning unit I37adjacent to the basic cleaning unit II, and both arc ends4131of the impactor413respectively impact a reaction cup located at the injection station in the basic cleaning unit II38and a reaction cup located at the injection station in the basic cleaning unit I37. In this way, the reaction cups can be impacted when the solution is injected twice, so as to achieve better impact and cleaning operation, improving the cleaning effect. In the present embodiment, no reaction cup vibrating mechanism is arranged at the first basic cleaning unit137, because the reaction cups are placed on the turntable and not or less affected by the magnetic fields before being moved to the injection station of the first basic cleaning unit I37, the adsorption force between the magnetic beads is weak, and after the reaction cups are moved to the injection station of the first basic cleaning unit I37, the magnetic beads can be dispersed by the cleaning solution, so it is not necessary to conduct vibratory impact.

As shown inFIG.31, each reaction cup25comprises a cup body251and a cup body flange252arranged on the outer circumferential surface of the cup body251. After the reaction cup25is placed in the cup hole32in the turntable231, an impact gap47is left between the cup body251located in the cup hole32and the inner circumferential surface of the cup hole32. Such arrangement facilitates the impactor to impact the reaction cup, which further improves the cleaning effect.

As shown inFIG.32andFIG.33, the needle body lifting mechanism24comprises a lifting plate241and a lifting motor242, the lifting plate241is provided with a guide sleeve48, the top surface of the cleaning tray barrel body22is provided with a guide rod49, and the guide rod49is in matched connection with the guide sleeve48so that the lifting plate241is slidably connected to the guide rod49; and the lifting motor242is arranged on the cleaning tray barrel body22, the output shaft of the lifting motor242is in transmission connection with the lead screw50, the lifting plate241is provided with a nut sleeve51, the lead screw50is matched with the nut sleeve51to form a lead screw nut mechanism so that the lifting plate241can be controlled by the action of the lifting motor242to move up and down, and the injection needles and the aspirating needles are arranged on the lifting plate241. In addition, the bottom openings of the injection needles are tilted so that the cleaning solution sprayed through the bottom openings of the injection needles can be flushed onto the reaction cups, because the reaction cups apply a magnetic field at the aspirating station, the magnetic beads are adsorbed on the inside walls of the reaction cups. After the magnetic field disappears, part of the magnetic beads are adsorbed on the inside walls of the reaction cups. The bottom openings of the injection needles are tilted to further ensure that the cleaning solution can disperse the magnetic beads adsorbed on the inside walls of the reaction cups, so as to further ensure the cleaning effect. The bottom side walls of the aspirating needles near the bottom openings of the aspirating needles are provided with notches to prevent the bottom openings of the aspirating needles from being blocked when the aspirating needles are inserted into the bottoms of the reaction cups, ensuring the normal aspirating operation.

The top surface of the cleaning tray barrel body22is also provided with an upper position sensor52and a lower position sensor53, and the upper position sensor52and the lower position sensor53are arranged on the top surface of the cleaning tray barrel body22through support rods54. The lifting plate241is also provided with an induction sheet55, and the upper limit position and the lower limit position of the lifting plate241can be detected by matching the induction sheet55with the upper position sensor52and the lower position sensor53. In the working process, when the lifting plate241is moved up to the upper limit position, the injection needles and the aspirating needles on the lifting plate241have been moved up into place and have been removed from the reaction cups. At this time, the turntable can drive the reaction cups to rotate. When the lifting plate241is moved down to the lower limit position, the aspirating needles on the lifting plate241have been inserted into the levels of the reaction cups, and the aspirating operation is ready.

As shown inFIG.34andFIG.35, the injection needle I271and the injection needle II272are connected to the lifting plate241through a limiting block, the limiting block comprises a base56fastened on the lifting plate241through a screw and a screw cap57threaded to the base56, the base57comprises a bottom plate561and a cylinder body562arranged on the bottom plate561, the cylinder body562and the bottom plate561are connected through a mounting through hole563, the inner circumferential surface of the cylinder body562penetrated by the mounting through hole563is provided with a groove58, the groove58is concave along the radial direction of the cylinder body562, one side of the groove58is open, and the other three sides are closed.

As shown inFIG.36andFIG.37, the bottom of the screw cap57is provided with a screw cap through hole571, the injection needle I271and the injection needle II272are provided with a guide block59respectively, the guide block59is cylindrical, the diameter thereof is matched with that of the mounting through hole563, and one side of the guide block59is provided with a bump591protruding radially. During installation, the injection needle I271and the injection needle II272are successively penetrated through the screw cap through hole571of the screw cap57, the guide block59and the mounting through hole563of the base56so that the bump591of the guide block59is stuck into the groove58, the groove58is matched with the bump591to limit the injection needle I271and the injection needle II272in the circumferential direction, the screw cap57is tightened onto the cylinder body562, and the bump591is pressed into the groove58by the screw cap57to limit the injection needle I271and the injection needle II272in the axial direction. With the above structural design, the injection needle I271and the injection needle II272are connected to the lifting plate241, and the positions of the injection needle I271and the injection needle II272are fixed, ensuring the normal injection operation.

As shown inFIG.25, during cleaning in the present embodiment, the turntable231drives the reaction cups to rotate so that the reaction cups are successively passed through the basic cleaning unit I37and the basic cleaning unit II38to complete the cleaning. Supposing that the number of rotation turns of a reaction cup driven by the turntable231is set to N2, N2>2.

When N2=2, the cleaning steps comprise S1 cleaning step for the first turn and S2 cleaning step for the second turn, wherein the S1 cleaning step for the first turn comprises:1). Controlling the turntable231to drive the reaction cup (not shown in the figure) to rotate to the injection station in the first basic cleaning unit I37, and stopping rotation;2) Controlling the injection needle I271located at the injection station in the first basic cleaning unit I37to move down to be inserted into the reaction cup to inject a cleaning solution A into the reaction cup, and cleaning magnetic beads in the reaction cup with the injected cleaning solution A; and after injection, controlling the injection needle I271at the injection station in the first basic cleaning unit I37to move up to the original position;3). Controlling the turntable231to drive the reaction cup to rotate to the aspirating station in the first basic cleaning unit I37, and stopping rotation;4) Controlling the aspirating needle28located at the aspirating station in the first basic cleaning unit I37to move down to be inserted into the reaction cup to aspirate the cleaning solution from the reaction cup, and draining the cleaning solution; and after drainage, controlling the aspirating needle28at the aspirating station in the first basic cleaning unit I37to move up to the original position;5). Controlling the turntable231to drive the reaction cup to rotate to the injection station in the second basic cleaning unit I37, and stopping rotation;6) Controlling the injection needle I271located at the injection station in the second basic cleaning unit I37to move down to be inserted into the reaction cup to inject the cleaning solution A into the reaction cup, controlling the reaction cup vibrating mechanism to impact the reaction cup, and cleaning the magnetic beads in the reaction cup with the injected cleaning solution A and the impact; and after injection, controlling the injection needle I271at the injection station in the second basic cleaning unit I37to move up to the original position;7). Controlling the turntable231to drive the reaction cup (not shown in the figure) to rotate to the aspirating station in the second basic cleaning unit I37, and stopping rotation;8) Controlling the aspirating needle28located at the aspirating station in the second basic cleaning unit I37to move down to be inserted into the reaction cup to aspirate the cleaning solution from the reaction cup, and draining the cleaning solution; and after drainage, controlling the aspirating needle28at the aspirating station in the second basic cleaning unit I37to move up to the original position;9). Controlling the turntable231to drive the reaction cup (not shown in the figure) to rotate to the injection station in the basic cleaning unit II38, and stopping rotation;10) Controlling the injection needle I271located at the injection station in the basic cleaning unit II38to move down to be inserted into the reaction cup to inject the cleaning solution A into the reaction cup, controlling the reaction cup vibrating mechanism to impact the reaction cup, and cleaning the magnetic beads in the reaction cup with the injected cleaning solution A and the impact; and after injection, controlling the injection needle I271at the injection station in the basic cleaning unit II38to move up to the original position;11). Controlling the turntable231to drive the reaction cup (not shown in the figure) to rotate to the aspirating station in the basic cleaning unit II38, and stopping rotation;12) Controlling the aspirating needle28located at the aspirating station in the basic cleaning unit II38to move down to be inserted into the reaction cup to aspirate the cleaning solution from the reaction cup, and draining the cleaning solution; and after drainage, controlling the aspirating needle28at the aspirating station in the second basic cleaning unit I37to move up to the original position;

The S1 cleaning step for the first turn is completed through the above steps.

The difference between the S2 cleaning step for the second turn and the S1 cleaning step for the first turn is step10), and the other steps are the same as those in the S1 cleaning step for the first turn, that is:10) Controlling the injection needle II272located at the injection station in the basic cleaning unit II38to move down to be inserted into the reaction cup to inject a cleaning solution B into the reaction cup, controlling the reaction cup vibrating mechanism to impact the reaction cup, and cleaning the magnetic beads in the reaction cup with the injected cleaning solution B and the impact; and after injection, controlling the injection needle II272at the injection station in the basic cleaning unit II38to move up to the original position;When N2>2, the cleaning steps comprises T1 cleaning step for other turns and T2 cleaning step for the last turn, the T1 cleaning step for other turns is the same as the S1 cleaning step for the first turn, and the T2 cleaning step for the last turn is the same as the S2 cleaning step for the second turn.

In the present embodiment, two cleaning solutions are used in the whole cleaning process: the cleaning solution A and the cleaning solution B. The reaction cup is injected with the cleaning solution A in the injection step of the basic cleaning unit I37, and the reaction cup is injected with the cleaning solution B in the injection step of the basic cleaning unit II38. The cleaning solution A has strong cleaning ability with more residual bubbles after cleaning, and the cleaning solution B has no residual bubbles. After entering the basic cleaning unit I37, the injected cleaning solution A is used to clean the magnetic beads in the reaction cup. After entering the basic cleaning unit II38, the injected cleaning solution B is used to remove residual bubbles in the reaction cup.

The two different cleaning solutions are used to cooperate with each other, which can not only achieve the effect of cleaning the magnetic beads in the reaction cup, but also remove the residual bubbles in the reaction cup.

“A plurality of” in the present embodiment means a quantity of “two or more than two”. The above embodiments are merely used for illustration of the present invention, and not intended to limit the present invention. Various changes or transformations can also be made by those skilled in the art without departing from the spirit and the scope of the present invention. Therefore, all equivalent technical solutions shall also belong to the protection scope of the present invention, and the protection scope of the present invention shall be defined by the claims.