Protein: AGIA_ASPFN
Sequence: MGSIGNVQSPLPRCVQSMHAPSVMQEEMIVSTIADPSHKSYFETYHFSAQGIVDPDQLNGAIHAVARKHAVLRSVFVHPTEFDSTNVQIAVLDQAYTLHRAKLLSLQPIGEEIRFGILPLESIGTDEWDGVMPWKFSLVVCEREQKSYITVRYHHALLDGWSARALLELVQQEMLNPGAVLKGSDFFSLVRQPLKRDWKQDETLLRERLAQIETSPILSPGPVANGDLRVGEVTREVSISSDIWLPDRPAVPARLLRLALGMTISVFRNSDDSLFLEITSARSRLFPKDQQVLGPVLAPQVRNIHLKEHTTLGECMQLLRSSNDPQHNFSVSQLKSFLTESSRDLDVCLVCQTNESYPSNGVGHWEWIKGEARNDLPFILEILPPKEGVFFAKIRYHQNRFEKQFADSFLEFFCQTLTWMQATGNSIDHQTFAAAVSEICRQGDYRQHYLALNPRGVSDDPVNVHDLIEQAASKWPSKIALEAEHNQFMTYAELSNESDRVAKGLRHCLPRDQKDQPLVPICFDKSVDMVVAIVAVLKAGGAYVPLDPSQPRDRLVSILSACHATVVIAGQTDLQDVLHSTCSELSILVTSIENLSEHDKSTDELSGSEHPPPSSLAYVLFTSGSTGTPKGVMVEHRNLVAFMKAGEGNADGTWTSTRLQLAAYSFDASIGDIFANLYRGGRLALVQRNKMLSNLNHWLEEMLITHLALTPTIGDLIISHLPPRLQTLMFGGEPFHQSFLAQAPAEARVWNTCGPTETVVDVACCILQKENPDVPIGRPFGQCQIYILRRGGSNTAVPPNAIGEICVAGPQVSRGYLERPDLTSLGFVTDPFRPSQRMYRTGDLGRLNHRGMLEHLGRADGQIKLRGLRVETGEVEVTIKQSSSIIAQVTVSVNHLEGYDREALVAWIVPEASQGSQDIELAWQEDIMPSCQRRLVPYMIPEVWIMISYLPLTVSGKLNRGTLASWVEHVATGGTHDGLYVITAPWKTQEITQRLPESPAERLLVATCANILGTTLHAVSMDSTFIALGGNSLLAMRLLAALRQEGMNCSLRDLLTPMTLGDVARAMSPSSTPKKQITKAFYHEDSWERIVADSSIAADTIDAIYPCTPQQEGLIQTSLHGDKSAYFATITVHLGDSLNLRTFHAAWNRLVFGCDMLRTAFVSFSEVQHPPVSESNILQVVLSQSAEDVRRLVSLDNRDIAFQFGVVPLSAGISQGSVNEPWRLHLKIHHALYDEAFLSRIVAELCIVYEALEMESSEAALPPSRPFSAFVESLCNDDPEVSKGFWKKYMHEVAPATWPVASGIRRMREENGQDVEMTVVKSWTGNAVALGQKFQATPASIVRAALALALAQYSQTDDVVFGEVSSGRFDHDRFTLGPCLATHPVRIQIDRKSSSGMLKLVTQALESYLATTPYQHCGLASIHRQTADPDLMAFQVLFAHQEAFVEYTAGSRFHVKSAKLRNIGFPLVLESRCDRSTGNLAFECSFDRRYLGDQDVDWFLRSICRTLDMFSAGARDGLSDQKLITGVVDEEMRRAIEKWSTKRTPFGSTFTRNAEICAHELFEMQADMTPQKIALQVNQSEFITYKELNKRCNQLSNALVNWLDSLGLEQSRDQQIVPLCFSNAVDMVIAMIAVLKAGAAYLPIDQNHPQERIQQILSLSGARAMLGDGEAETLEKLQAASKQANSTLITSKRLRTLYGGQTAKKPSFRPTPASLAYVIFTSGSTGKPKGVMVEHGNLAAFMQANEPEAVGTWTSVRLQLATATFDAATGETFGTLGCGGRLILGQTHEILAGLPDWLERTNITHLFVTPRVAANFLTEIHPPYLRTLHIGGEAFDPSILPHMPPGCDVYNVFGPTETTIYATHYKIRKGDGIRRNIPIGYPFGGCRLYILNPETLEQVPIGVIGEICIGGPQVTRGYQGRPELTSRSFLSDPHIVGERMYRSGDLGRLCGDGSIEHHGRIDSQIKLRGLRIEISEIESVCLEHAIATACTVIVLDREDGQVLVAFVQTQKDQQTSTCSQDWAETESILHRHLDSRLPSYMVPSRFVPIEVMPLTTSGKVDRRQLGARAETMDQAGELFTSQHTNHAGSWEQGSIEDKIADAWVQVLGIDRANITPNVAFSRLGGDSIRAIRLLSLLRKAGLKLNMTDVSNASTIFSQAKCATSHTVAAKKSTEDATDIDAHTGPVALGPIAGRYAGIQLKYASQRGEQIIDHFNQSVLLDVTTLCPLRLQQALQRLRNHHDPLRAIVHWSLDIPVEEWTIRILPCSDIKPLVLDSPRTLTLQALREQIQHRITGLDIRRGKVMDAELYRLDGDSRVFLFWTVHHFVVDIVSWQILRDDLNVLVRAKEEPESIALQPATMSFLAWTREGQMKSQDASLATGSIPEEPDILLSSSDSDQLPLWVRQPELVPIHPVNRTTTSASLSPCITALLLGRSNNVLSTEPLDLLLAGLAMTISKHFARAVDRLVVGLETHGRHTGTNTADLSRSIGWFTAIIPMILDCRCESSSIESVVRRVKDQRRLLMENDRGFRHFVASRWSTANARKSEIMPLVFNYQGVRHDPHDHDEMMLHPVQIPGLSWIESSPHAIPLSHAAFELYVHDRQAYIEATWPSDGEMDQEDVATLMGEQITNICQYLAEKDVLGKTSISASSTSAFGLLPDDCFDRVFSLYPEDTLDDIIPCTPMQRALLYEGIADHESRSYVTCRIWRIPTDQAICSQIEGAVKSLIQRHGILRTVFHIDPEVGPLALVLRDTQSPTASAVGHVKVKDHTEMEERVTSLLCNPDYGNPMKQAFYVRIVHAADGSGARLIWLLHHSLIDAWSQDLLLSELTQILVDGCPTKSLIPRPSFGSFARYVTSDSRADHSHGKFWSETLNGVQPKSLPLSLMSSSPINAAAVVVEQACNLAILSENCISPAALVSLAWSLVLSEILDTDDVTHGMLFSGRQLPVDGVADIIGPCISTVPIRTHLNRHGKVLDLLQSTEAAIRLAGSHSTVGVDGVARAAGVEAPALVNTLLNFFGVRTDVLENDGLNSILELDSVDDGLPPSITLSCWQREVDANIMVMRLERRHPLQIGIAQCLVKRMAWYCHTLSHHMDRKLDSVLSITSNEDQLLTKWSQPVDSRPSDSYPCIHDLITGWAAQVPEKVAVQVAESQFVTYGEIEKKSTAIARVIERLVDPRSGSTPPLIPICCDRGVDMVIAILAILKAGAAYVPLNISDPEGRLEAILRQTGSTILVDGLLDKGSRRKLHALGDRTSTTVYTVDGLSILPPSEVSLRKAHSESLAYVLFTSGSTGEPKGVMIEHRNLTSFITTQHNEIIGRWTSCRMPVAAYTFDVSMADLLISLAIGARVALVDSEKMLASMPYWADRTLATTLSMTPTLASLLARSLPPHVAVLMLAGEVFDPNIMKALPRECRVWNGYGPTETFYASFHPVDAQPTHAQVPIGRSFGGNRIYILRPGSNYRQPIGAIGEICIGGTQVARGYLGREDLTSRSFTRDPYNTQTVLYRTGDLGRFTDCGVVEYLGRMDDQIKIRGQRAEPAEIESVVHAASPRVAHAIVDLYQSKRGGGLPRLIAFISTKDPVPPSLCKTFLQEEVEPSCRNQLPGHMMPSTWIYVRTVPLTSSGKADKRMLRSWMARLEEGETVPEASIIELSLSTQMNSETHQDISGGPESPTEIMLRQSCAQLFKVNEDIISLDKSFISSGGDSLLALQLNARLREKGLKCTPRDIVEAQSLAELATILNTSYEAVSPVHMADWTLDLNEMARLPDNGIQGWETIVQRVGIDPGQVRCVMPCTPFQEGVLSSNDESGSSAGYLAHMTVGLGKEIDVEALKYAWQETVDHEDMLRTTFIPADMDMTDIRGLGQGSSLLQVVLYPESPQAGRVKTMKTVSTPATPNAALPSYPSLQGKAQQGHIPVAALVAIGSQDGEGQECTLLITMHHALYDEAYLSLLLKDLSGRYRSIACNEVVPQVPEDQRIPFSTYVRFVHSKLGTAPSTSTAGKFWKSYLADATPSTWPLPHGMQSSITSVKSPETAVLEWTGNLRAAASKVQVTAAAIARAALALTVAEHTNVTDVVLGEVSKGRPDIRGPGDARARFITGPCATTHPVRIRIADEGASKRRTMLQLLRESFTSYMETLPHQFYGLSRIREQSCRVDLLPFQVLFVYQDAFRQKEGLAGDDAFQIQGGNLGQMGFPIVLECSCLSGDSGVVFHCTYAPDVIDKPRIEWFLHHISQSIDALVQVDPARSDSLRSARVPLSAQETRQLELWSRCHAAKDVEKVDDTMPTETSSITHAFDDTARNLHEKRSTSLSMALQRHIVSLDDVGSQQPIIPICFERSTDMVVAILAILKAGAAFVPLEPGYPPERLISIVRTARASLMICGKEDKSNEILVSVCHATNTKLITLEDLKSRPASSDQAVISRYCRDDSRIAYVLFTSGSTGTPKGVVITHRNLLAFMRHNNPDVHGRWYNSRMPVASYTFDVSMADIITTLCCGGRVVLVPVQKLLPSLGAWVDASITSHISLTPTIANMLWEPVKNAEIAFPFLSVLLLAGEVFDAQLMSYVPKECRVWNGYGPTETFYVTFYRVPKAHAKEQTSVSIGYPFGENVIHLLGFESNDHVPVGCIGEICVMGPQVAQGYLGQPELTKQRFKRGVISQAPEGFLYRTGDIGRFHPDGKLEYLGRFDRQIKIRGQRVELAEIEMAIAQHSFVDGCAVVVVNTPTGDSLVGFCVKTSSQTPGKGWDANTAVQIKTWISTRLPAHMVPSYLFPLEGELPRVPSGKVDRQLLAQRATDLLADSLLSSQGQDTYIAPTTEREKVICEIFEETLAQRVSVLDNFLHLGGHSILAIRAVSKINHRLHANLTFKDVFDFATARDLARQLESTATNHRSYTSIPRLTQDKMIVRQSFAQGRLWFLDQLHPGSTWYLMPFGLRIQGDLHLDALEAAVSAIEERHETLRTTFEHRDGENVQVVHPFAHRQLRVVEVPPAVDEEGLLGALKEEQSTPFDLQVHPGWRPLVLRQNKRSHILSIVIHHIICDGWSVAVLLKELSTFYSAALHGKPIHAQLPPLPIQYRDFSAWESQKEQRVEHDRQLKYWIEKLTGSKPAEFICDKRRPQAPSRQAIFEEVRIDGAMYDQLRQYCKQHQLTPFIVLLAVFRATHYRLTREADATIGTPIANRGREELHDIIGLFVNVQCIRLKVDDHHTTFEDLVNQAQSTATEAFAHQDIPFDRIVSALQPDRETTQNPLVQTVFAVHPQTQGKEELEGLLTEQILLSRTTRFDLEFHLFQEEDGLSGQVVFAQDIFFPETVKAMISVFYAVLECGLNQPSISVASMALLNDLSMHDMDDLLSINQTDYPRDATVVDLFRQEARSHPDSIAIVHEGKEVTYGELDRQSDNIERWLRSLHLDRETIVGVLAARSAEAITVFLGIMKADLAYLPLDASTPQTRICSVLSCISERITVIVVDGAQVAVPDVSLAHVDFVALSNLLDDQRHKPSDNQKFESESSISATSLACVLFTSGSTGRPKGVMIEHRAIVRLAKDFNFAKAAGKPLAHMASLSFDVSTWEVFMPLLSGGVVVCVDAMTVLDYKALSDVYARHHVRAAMFTPALFKQCLHDSPSIVKNLDLLILGGDRLDPEDVFQAKQLTQGIILNGYGPTENTGASTIYPIPDEESCVNGVPIGKPIGNSGAYVMDDSLNVVPKGVVGELVVTGDGLARGYTDPEKNEGRFVHVVIGQDTVRAYRTGDYARWRPIDGQLEYFGRRDDQVKIRGNRVEIGEIEHNILSHSAVRSAAVVMNGVGAEADLAAFVTLHPIHDDVAETERVDAWKNVFDTEAYDSFTHQPNRLGRDFVGWLSMYDGCNIDLTEMDEWLDDTLQTLLNGQDPGKVLEIGTGSGMILFNITKGLEEYVGIELVPKLAHMVEQVANADQRLAGRVKVHPGVADRVEDFIPGFIPDLVIINSVAQYFPSAGYLSEIVQKLVRLQGVKSLFFGDIRSYPLYDEFLVSKALHNAGARATQDQIRKCIEESKAAETELLVDPAFFTSLTNQFPDQVAHVEILPKLMNATNELSCYRFSAVVHLKHATAATRIACQVKKADWIDYTARSLNSRSLLEHLRSSHDNTIIAVENIPNRRTILERYIVDALKEGVEPRSMDTKSHWQVRHRNRAAQSSALLPGDLVAIANQAGFQVEISWARQSSQRGAFDAIFHRMGPNSRRQRVLFDFPVDHQCRDVDSFTNHPVQGQQDQQCIFELKESLRAQLPAYMIPRTITILDQLPLTDRGKVDRQALRQRIIKQEPVATESESSAGRVTVEYGSEMERILCDVFAEVLGLQSVDRESSFFSLGGHSLLAPRLASRVSKRLDCTATVRDLFDCPTPVRLADRLLSKQSDSNTEANTSTDGKTQHSALDKVKYHNTLRDWGVQSSEVGHLMPCTPFQEGVLSNSLAVPGDSGYLSVVRLGLQSQLDTKAMRLAWQKVVEREETLRTAFIPVAEDLSSACITSSTFWQCIFNINSREVQRLLCIEGRNSGVDRSALGFGHIPVSLILTDVPTVCKARGVGSTQLELTIHHALYDEAYFRWIIHELSREYHKARLAKDYVPLRAPQTSMNRIPFSIFVSQLQAMPKESATSFWKSYLNGAPAACWPVARGLESGRITEIDEFSSRSLIWKGNMHNLAGARGVTPAAISRAAVALVVAEHSGVEDIVLGEVSSGRSITDGAAGFVAGPCISTHPIRIRMQQRQGSRSSQHRLSFDQLVKQSLNSYLETVPYHQLGLPSIRRQSDAPDLLPFQVLFVYQQAFDFETDSREEASHNFKVQGGHLGRFEFPVVLQASCHPVTGHMSLQCMFDPTVLITEDIEWFLEHISQVLSSIADSSSQPVARLTVGDAEEAALTKLSCAKDQTPELPLGTDSESLCAHDLISRQAMESPCKIAVQYELSQFMTYGELDRESTKLSIVIRAFFDHLSKSVSHEQPLVPISFDKGLDMIVTMLAVLKAGAAYIPLDISHSEQRLRMICQSAQAKLILWDGQNGFDKLRAIGHSSGATISTVDELSDAVDGWGSTPRSGEKPNLSSLAYIIYTSGSTGVPKGVMVNHANLVSFMRSATNETYMSWTANRLQIASYAFDMSVSDIFPVLAVGGRVLLARQQSLWSDLAGWVDAFAVNQLMTTPTVADMMLSSALSDGFLLAHLRDVIVGGEAVKRDILDKAPTEMVFWIQYGPTETTVVVTGCMFRGPTYYQPVPHSQITTIGFPLRGCRVYILQPGTSNRVPIGVPGELCISGPQVTMGYRGGGDPSESPFVPDPFWAGQTMYRSRDIAKVHGDGMIEWVGRMDSQVKLRGLRIDLGEIESAARQLNGVQSCAVVKLALEDKETLLAFIEVAKSHQTHITPVTIQQHIAQNVPSYMVPAHLRLLDKPLPRTASDKLDRKGIHALAQQLVDNGDLLSSCTSRIPPADLRPSPGTLEATLASYWGTILGIDQEVISLETPFSHLGGDSVRAISLLALLRRNNFQLNLTDLGSFSTIRSQAFRILCDVQPQKLPAYMQLTTRSTSRATMVLIHPFFGQSSVFDHVVPALSEQYDIVQVSDPFFGKPDGPASLRDWAAHYLEALHVHLEQDRPVVLVGYSFGGLLVLEMARLLEMTGKGSPFSTVIVDTRCYDPDQPFFKDEEERQTAADDAVRLFGPGQTSMIEEHFDKHAHIWENSVCPDKYLGRSLYLATPEAVESGIVDWWRNQCPHIEVQRVECSHGEIFEPAMTGRVSALINGHCDLDFTRIEP
What is the function of this protein?
Nonribosomal peptide synthase; part of the gene cluster that mediates the biosynthesis of the aspergillicins A and F, 2 cryptic cyclic hexa-depsipeptides (PubMed:31117395).  The hexamodular NRPS agiA catalyzes the condensation of the six amino acid residues including N-Me-L-O-Me-tyrosine, L-proline 1, L-proline 2, D-isoleucine, O-acetyl-threonine, and L-isoleucine (PubMed:31117395).  The starting condensation domain (C1) of agiA probably loads acetyl-CoA which is condensed on the N-terminus of threonine by the first module to yield O-acetyl-threonine (PubMed:26273902).  The second module then loads L-isoleucine.  The epimerase (E) domain on module 2 is probably involved in the formation of the D-isoleucine moiety (PubMed:26273902).  Modules 3 and 4 further load 2 successive L-prolines (PubMed:26273902).  Module 5 is then involved in the condensation of O-Me-L-tyrosine produced by the O-methyltransferase agiB and the N-methyl transferase (NMeT) domain on module 5 probably catalyzes the N-methylation to yield the N-Me-L-O-Me-tyrosine moiety (PubMed:26273902).  The A domain of module 5 loads preferentially O-Me-L-tyrosine, but it can also accept L-phenylalanine, which leads to the production of aspergillicin G (PubMed:26273902).  Module 6 then loads the last residue, L-isoleucine (PubMed:26273902).  The C-terminal thiolesterase (TE) domain probably cyclizes the peptide using the hydroxy group from threonine to form the cyclic depsipeptide (PubMed:26273902).
What are the official names of this protein?
Nonribosomal peptide synthase agiA, NRPS agiA, Aspergillicins biosynthesis cluster protein A.
What is the protein family that this protein belongs to?
Belongs to the NRP synthetase family.