Protein: GLOA_GLAL2
Sequence: MTPSRSLENGEKQMNWNESPQTASPKNVLRDSNSNGNYVNGHGTNINGDGSDGVGNGINANGSATKINGNGTYTNGNGAHTNGNGVHTNGHGISLESQTSDSKVHSTSKFKEEFRAICAKVLKIDIEELDDTCSFVSLGGDSISAIKLVTECEVRGIELKTVDVISTHTISGLFATANFRPFGKHSNGKTGISYSRNQEDDDPSPFALWTAHRDSDLIEKRQRLEEIAVLCGVETEEIEDVYPCTPLQEGLIAITTRQPTAYVQRRVCRLTNEIDLERFWAAWETLVANVASLRTRIIMGPGGQSLQVVIREKLLWRRGSNLGRYLSRDRADGMMLGQPLARFGRIQEETGSFFVWTAHHSIFDGWSALLLYRQLLAIYQQSYVPRLVPFSRFLRYLAEQDSTAATRYWHSQLHGDTMADWPALPSSNYQPQPQHVFRSSINLPHGYKPGTIMISNLLRAAWALVMAQYSGNDDVIFAVTVSGRSAPVSQIADIIAPTITTVPVRIRIDRSMSIAELLLEIQSQAAKMIEHEHTGLQTIKKLLPEFGTALELRNLLIVQPEAESDDYVYKEFPGLEAIREAMEDFDNYGLNVECILGSQSIEVLVNYDDHVINTMHLRDVMGQFTYTVQCLCNPSVSKLSVNDVATIKTSDQQRILEWNEHIPPSVDRCIHHLVQDQVNIQPAKLAVDAWDGKYTYADLARESISLAHHLVGLGLGPEQPVGLCGSVLPLGVSHPFARTSGIVQEAKVRIVLVDESQRTELAKLATTLIVVDSELIAALPSEAKPPETGVTPENVAWILFTSGSTGTPKGVVLQHASLCTSLIGHANTVGINKYTRTFQFAAFTFDVSLCDIFSTLQAGGCVCMPSEDERMNSLAEAASRMEVNYAELTSTVTETISPSQVPSLATLALSGEALKPSVLSTWARHSSVFNSYGPTECSIVASNSKRLSNVEEAQNIGGPMSSIFWVVQAANFHQLCPIGAPGELLIEGPLLARGYLNDEVKTEKAFIIDPDFTKQLGLSPGRRMYRTGDLVRQNQDGSLMYLGRCDSTQVKVRGQRVEVSEIEYQISRQLPEIQTVAVEMLQRGTQASLVAVVNFAMDSKYAAPAAFDTTTTKTETIFSTDALRAVFQDLQLALSQVLPAYMIPTLYAPMSTIPMNASGKLDRRVLRTKLDSMSFDELRVYMADDGPKAAPSTDAEKQVQSLWSEVLAINPQDIALSDNFFRIGGDSIAAMRMVALKASRRLRLTVADVFQHPQLSDLAFVIQRRLQMVEDGVQEEDSAPFDLWAKFKVDGLDSTKRAQELAIIATRCDIRVDDIEDIYPCTPLQEGLIAITTHQPTAYVSRQIYKLAPTLDVVRFQKAWQTLAQVTPILRTRILAGLDTSDVSLQVVVRGSITWQYGADSGTLSDYVAQDRKSGMRLGQPLVRFGLVRNSSEQFFIWTAHHSVYDGWSVSLMYQHLYDIYFDQRIPSTIPYARFIRYLIRHDDAASEKYWRSQLQGEVVSNWPPLPRADYQPRPQQRYTCDIILPDHTMNDRVNSLLPSVLRAAWGVTMSKYTGQGDVVFGVTLLGRNAPVSQITEMTGPTITTVPVRIHLDGPQPLTINQFLQNVQKQAADMINYEHAGLQVIKKLVPELNSSLELRNLLVIQPASETDGTAFPGLDPLPVDLEGFDSYGLTIECSILSGLVKVEARYDENVIATPQLKRTIRTFEHVVKQLLDVRNSVYRLEEMSWLSDYDEEAIANWSKATPIRVERCIHELVQEQTKLRPNATAICAWDGNLTYAELDMQATWLARYLTSLGACSQRMVGICMDKSKWAGVSMLAVLKAGAVLVPLGVNHPEARIKAMVDDTDTQIILVDEKQRDRLSIQGVRLITVDADILKKLPVLAEKEELLGSVNPDDAAWVIYTSGSTGKPKGVVLQHVALCSSIQAHGARFGMGTSTRMLQFAAHTFDACIQDYFTTLSWGGVVCVPSENDRMSDLTTAMCQMKVTFATLTSTVARLIDPHKVPSMQKLALVGEPVKADVVKQWLGHTIVLNAYGPSECSIHSSCGEPLADSTKSAVIGTGMGTRLWVVDVDYNQLCPIGAPGELLIEGPLLAREYLNDPRKTKAAFVSDPRFAQKFGLAPGTRMYRTGDLVKQNEDSSITHLGRRDTQIKIRGQRVEVGEIEFQIAQHPQVRTVAVELLEQDSNGSQVILTAVIEFTEDSEYRNGPVTSSGLLTLTPSLSLAFEMLRGALFQVLPSYMLPSMYVPIVDMPMNVNGKLDRRAVRDLLQAMTPDVRQQYLSASDHKVAPSTREECLVHSLWTEALSLSFSQVGIYDNFFQIGGDSVVAMRMVATESARELQLTVADLFQHPRLIELAELLAKRSVDKKVEADPEPFSLWLEPQIGSEQQQEKLTMVAKQCGISVNHVEDVYPCTPLQVGLMAITARQPLAYIDRQVYKLADTIDLDRFQAAWRALSDATPILRTRIITSEGPQSFLQVVVGGCDPWRDSNDLEDYMASDRDVGIALGKPLVRMGLVRERNSEERYFVWTAHHSVYDGTSALLMYQQLASIYFHGSLLPTAPFTRFIRYLARKEVIAAESAAYWADQLQGEVMANWPPLPRIDYQPKPQHEMTQIFRLPQFESRSVVTISNVIRAAWALVMAQRTGHSDVVFAVTVSGRNAPISQVDSIIAPTISTVPVRVQIDWTQDVAGFLFAIQNQAAQMIDYEHTGLRAIKALVPELGPTLDIRNVLVVQTAEERGAADHFPGIEALPQGKENFDSYGLLTECTLGTDGEVRIDFRYDDNVIPSSSIKRISAQFAHLVQQLCGNATSTLHRLNELVLIAPEDQEQIMKWNPMLPPRVDSCIHELFYKQVMARPQAEAVSGWDGELSYSDLADESIRLAYQLISLGIGPEMKVGLCIDKSKWAIIAIMSILFAGGVVVPLGVTHPLPRLDVVIEDASIDLILVDQHQRKRLAALSQNVKLITVNDALLRTLPVHTEPPVTGVVSRNAAWIIYTSGSTGTPKGVVLEHGGCCTSMRTQGKKMNLSAETRALQFTPFTFDVSISDVSATLIYGGCICVISESDRVNNLPGAIREMKVNFASLTPTVAQMLSPAELPSLKTLALTGEAVKPEVVELWMNSVALYDTYGPSEGSVCTCNGPLSSPDQADNIGFPMSTLHWVTQLHNHNQLCPIGAPGELLIEGPLLARGYLNQARTKESFVNDPAFTKQQTGLPSARHIYRTGDLVRQNEDGSFIYLGRRDDQIKIRGQRVEVGEIEYQIVCELPGTHSAAVAMLQDGKNISLIAIVDFKSDSEHYPGELESLGTLAPTPQLRAAFNELRQSLTKLLPSYMVPAIFVPVVQMPTNISGKLDRLGVRALLRAIPSDHLARYMIDETLPSETPSTKMEKVIQSLWAEALDIPMDNISAHDNFFQIGGDSVTAMRIVAATTRTNQLQLTVSDIFQNPKLSDLASVMTEHRKNHFDVMDEDPEPFSLWEAVISDNSNEQKRQIEAIAQQCNVSVDDIEDIYPCTALQEGLLAVTARQTSAYVSRQAYVLSDQIDISRFKEAWRKLVAGIHILRTRAVVGPDSLLQVVVRDEITWRHGSNLEDYIQQDKEEGIRLGQPLSRYGLVQLPSGEQVFVWTAHHSIYDGWTIRLMCRQLISLYRQEEDISTSIPYSRFIQYLTQINIEDSIEYWREQLRGESVTANWPSLPQPNYEPRPRHLLRKHISLPRTENQGIVMSNILRAAWGLVMIQYSGENDVVYAANLSGRNVPVRDVAEICAPTITTVPIRLRLDHTSTQTVGDFLQNIQQQAIEMINHEHTGLQVIKSLAPELSDSVLKLRNLLVIQPAAESDTHLDFPGIELVPSDIADFDAYGVNIECTLGQEIAVEARYDENVVETPYMNGVLDQFVYIVGLLCDPSISRWNATVPERVTKCIHELVQEQALARPTALAVQAWDGKLTYGELDNLANRLAHQLVSFGIGSLPDQMVGVCMEKSLFAVVAMLAVLKAGGVVVPLGVTHPITRLDTIIHDTGITVLLVDASQDERLAELSPTRILVNSDHLYHYLPARTQPPKTPVNHMDAAWVIYTSGTTGTPKGAVSEHGTLSTSIKAHGARYGFGHHTRKLNYAAHTFDGTIEDFFTTLSWGGVCCIPSEEDRMDKYKLMEFMNLTKVNSAAMTYTVASLLSPRDLPTLHTLVLGGEPATIDVVSTWMTEVNLFNCYGPSECSIFSAAAGPTKNTNELHNIGFPIGTRLWVADIENYHKLAPIGAPGELLIEGPQLARGYLNDESKTSAAFIVDPAFTTHFKLPLGTRMYRSGDIVRQKNDGSLVYVARRDMQVKIRGQRVEIGEIESQISQHISEARTIAVELLKLGTQSQPVLVAAVEFADGSQYHTGDVTSFGMLAPTEAIREAFIKLRGTLFQVLPGYMVPSAYLTIAEMPRNISGKLDRKTLRTMLEAIPADAIQQYLDGEAKTLPSTQVELQLQALWAEALGISVDGVGAHDNFFQLGGDSVAAMRIVAMSQAREMGLSVADIFAYPRLSELAVILDGRKNSNEVFYSDPEPFALWPRATNKVLDENTLLADIASKCNVTVNQIEDIYPCTPLQEGMIAITARQSAAYVSRQIYALDTTVIELGKFQRAWQVLANATPILRTRLVITQNGQSMQVVLRDTIAWRHSTDLDAYVNEDRAEGISLGQPLLRYSLVKQITGECFFVWTAHHSIYDGWTMRSICQRLVEMYNNIDNSSYQMPQSVPYSRFIHYLTQSDKSAAATFWRQQLHGDIMADWPSLPSIDYQPKPQHRDRKTIRVAGSTSKNILTSNILRAAWALLMSQYTGHPDVVFAASVSGRNAPVWQIGEIAGPTLTTVPVRVQAKPGMTVRQFIQEVQEQSTAMIRFEHTGLQNIKALVPEAAMALELRNVLVVQIAEESDHRIDFPGLEALPMPFEDFDSFGIHLECTPGLDDIEVEARYDVNIVSAPHMKRVLNQFEYVVQKFHDSEYSDFSLQSIQLNPHDERQILEWNATVPSHTERCVHNLVDDHVAARPMAPAICGWDGDLTYRELSRIATSLAFHLQHELGVGPEQKVGVCMDKSKWAVVAMLAVMYAGGVVVPLGVAHPLTRIRGILIDSASSVVLVDATQRERLVDLHTSLICVDAKLIARLSSQNQKQNQTQKLQVEVTPGNLAWVVYTSGSTGKPKGVMLEHRALSTALQAHGSAFGMDTNTRTIQFAAHTFDAAIQDIFTTFSKGGCVCIPSEHDRVNNLTKAMASMNVNFANFTSTVASMLVPEELPSLKTMILAGEAVTPTAVGLWSQHVTIFNSYGPSECSINSSCSKPVKEVSQASNVGLPLSCCFWVTNTTDYNSLCPIGAPGELLIEGPIQARGYLNDKEQTNKSFVTDPGFTKKLGLSGRRMYRTGDLVRQNADGTLTYLGRQDLQVKIRGQRVEIGEIEYQIKKKLLGARTVAVEKIEQGGHSEQTRLVTMMDFKDTSEHSHNPDILASGALSPTPKLQTAFEKLRQSLSEVLPSYMVPTFYVPVAQMPVNASNKLDRRAVKAVLASLTPDALQQYLPGGTDTKQAPDTDLGRLIQGLWADALGISTDTISMTDNIFHLGGDSVTAMRIVAAAYSHELQLTVTDIFQHPQLADLVNTLSNRSLERNTEIQEDPMAFELWEEAASCSTEERKRLLTEVAAQCGVAVSHIEDVYPSTPLQEGLMAITARQPAAYVSRQVYTLAKTVDQLKFKMAWQALSSEAHILRTRLLVAPHGLQVVVNDRIDWHHGTDLENYLQADRRAGMSPGKPLVRYGLIKQPSGETFFVWTSHHSLYDGWTLRSLGKRLLDLYNDGSPQSFVPFSRFIRYLQFGRPGNDDTATYWRNELEGDIVTDWPSLPRSDYQPLPRDNFSRAITLPDSHHSGSVVMSNVIRAAWALVMSQYAGHNDVAFAATVSGRNAPVWQIEDIPAPTITTVPLRISVDPMQTVAEFLDTVQQQAVRMIDYEHTGLQGIKALAPDLGPAIDLRNLLVVQPAADSDSNVQLDFPGLGSVSMPIEPFNSYGLTVECKLASHEIVVDVHYDKDVISSAQLKRVIDFFACVVQRILTQSPRSYRIQEIVAIGEEDLQQVLAWNSTIPPNVDKCIHEMVQAQVKKSPAALAISAWDGDLTYEEFFKSSARLAHHLVALGVNTGSNIGICMDKSKWGPVSMLSIMQAGAAIMPLGTSHPLARIETIVRNSEASVIIVDEKQRQRLDQLYTETSLTLVTVDSKFFKQLPAQTKAPSTGVRPSDASWLIHTSGSTGVPKGVIIDHVTMSTSLRAQGSWLGLNQKSRFLQFSNYTFDNVITDTFATTVFGGCVCVPSEDARMNNLPGFMATANVNVAMLTSTVARQISPSQVPSLHTLILTGEPVRADVVSTWLGHADIYNAYGPTEGSMSTCTKPMMRSDQVSNIGYPLATRAWITQPDHIQLSPIGAPGELFIEGPLLARGYLNNPEMTRDSFIINPEFTKRLGLENRRVYRTGDLVRQNEDGSLIYLGRRDLQVKIRGQRVEVGEIELQIIKHTPGAELVAVELIQQKDTKEEKRNLIAAIEFAKDSEHCHGSQNTPGPQILAPTDALRDDFARLRGLLYQVLPSYMIPSAFIPTTNLDRNLSGKLDRKGLRGLLEALSSQQLRQYSASGGSKVNPSTTMERQLQTLWAEALGIPADQVGAHDNFFQIGGDSMVAMRVVAASHSKDLNLRVNDIFQHSCLSDLAVVLTDRLAHNFNGGQDGHAPFSLIKTDDIDDFLQQIASSVVGCAIQDIVDILPTTDFQSSIIDTALAAPKSGTSHFLLDGNGPCDTRALKKSCLELIQATDTLRTGYVFDQGNLLQVIQAYFEPEIKIYETDSTIEAVTEDIVSRDMYQPIGLGRPFTQIAIIRETATLKHRVLLRLTHAEYDAHSMGSIWQNLRSLYEGGSTRPQAKFSDFLYNQRQSINADTYDYWRDLLKGSSMPAINLSAKKIGQYPSKVNQDLFRTIETPDLMVEGRTSAMLVKSAWSLVLSQFLRVNDVVFADTVSTRTTVDSSLMDAMGCCVTLIPFRVTLEQQWTIKDLLDNVRDQQSQSMQHSQLGFREILRECTDWPASTRFTSALNHISSGPESSIFSMRGVEYSISEMEIKDPLWEIDVGITTIQRGSELEIRLSYLPANISESVATSLLDALHNTLQFIHNNPLSPVKQVLSFHTDMKIQNGSSN
What is the function of this protein?
Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of pneumocandins, lipohexapeptides of the echinocandin family that prevent fungal cell wall formation by non-competitive inhibition of beta-1,3-glucan synthase (PubMed:23688303, PubMed:25879325, PubMed:27705900).  The 10,12-dimethylmyristoyl side chain is synthesized by the reducing polyketide synthase gloL/GLPKS4 (PubMed:27494047).  The thioesterase gloN/GLHYD exclusively interacts with gloL/GLPKS4 to maintain turnover of the polyketide side chain (PubMed:27494047).  The 10R,12S-dimethylmyristic acid is then transferred to the first thiolation domain of the nonribosomal peptide synthetase gloA/GLNRPS4 by the acyl-AMP ligase gloD/GLligase, followed by its acylation to L-ornithine to trigger elongation of the cyclic hexapeptide (PubMed:27494047).  L-ornithine, 4R-hydroxyl-L-proline (generated from L-proline by the dioxygenase gloF/GLOXY2), 3S-hydroxyl-L-homotyrosine (generated by gloG/GLHtyB, gloH/GLHtyA, gloI/GLHtyC, gloJ/GLHtyD and hydroxylated at C-3 by the dioxygenase gloM/GLOXY1), 3R-hydroxyl-L-glutamine (generated from L-glutamine probably by the dioxygenase gloE/GLOXY3) and 3S-hydroxyl-L-proline (generated from L-proline by the dioxygenase gloF/GLOXY2 to yield pneumocandin B0), or 3S-hydroxyl-4S-methyl-L-proline (generated from L-leucine by the dioxygenase gloC/GLOXY4 to yield pneumocandin A0) are sequentially added to the growing chain (PubMed:25270390, PubMed:25879325, PubMed:25527531).  The last C domain of gloA/GLNRPS4 is proposed to be responsible for cyclization by condensation to form the peptide bond between L-ornithine and 3S-hydroxyl-4S-methyl-L-proline (for pneumocandin A0) or 3S-hydroxyl-L-proline (for pneumocandin B0).  Finally, the subsequent C-4 hydroxylation of 3S-hydroxyl-L-homotyrosine and L-ornithine dihydroxylation at C-4 and C-5 are performed by the cytochrome P450 monooxygenases gloP/GLP450-1 and gloO/GLP450-2, respectively (PubMed:25879325).
What are the official names of this protein?
Nonribosomal peptide synthetase gloA, Pneumocandin biosynthesis cluster protein A.
What is the protein family that this protein belongs to?
Belongs to the NRP synthetase family.