Protein: ASCB_ACREG
Sequence: MTVNGHHTNGVNGANGTNGHANGSNGINDTKAVKEIVPFVKPQVNFASAQRLEGCIHSLPELVDFNSLNNQHHTFCVQAKSSEPFDTITHGEFKVAVSKCAAWLKENLPIRPSSDDKALTKMAPVALFMESDIGLVIHEFALMSIGVPPLVLSPRLSPVAINALLEATGAASFIVSPRMSEPLKGALAALAAKGVSTHIGNPYKAYYQPGADPKSVAPFEVPQNPEDVILLLHSSGTTGLPKPIPTTHRQLLFAVNCHKFDTEEQAQSLNLSTLPLFHGFGLVAPGLSMSAGKPTLYPASDGIPNAKSIVDLINKTNAKSMMTVPFLLDDITNLPNEEGIKALVHMDFVGTGGAALGAGIGDRLAKGGVKLLNFYGTTETGPLSLTFAPTDNYDWKYFRLRTDCEYKIDELEPRDGERRFRLTVYPYGSEGFEISDQLIRNEQYPETDFAAVGRDDDVIVLATGEKANPLILETKLTEAPMVKAAIAFGENQFNLGVIVEPAEPLTPDTESAFRESIWPIITAACDQMDAFSRIPSPDAVVLVPAGVVIPRTDKGSIARKETYALFDKQIKGVYEQLLKAAADAVEPLDLDNLEQNLKSLIQEHLHIQAPASDWGVEDSLFDIGVDSLQVLQLRRILVTAASKTEAFKDTDCEKMIPPEFVYMNPSIREIAAALTKGSDGGDVSLEDAAKEVVELAETYSLKGVSAQEKAPSSSEGAFVMLTGATGSLGSHVAADLARRDNVAKVVCLVRKDKGTNQPPMPGGNPFDKKILKARGIQLTDEQFGKLATLEVDPTADKLGLIPMAYGMMQAKVTHVIHAAWPMNYLIRLRNFQYQFKFLRNLLEFASQGPAPTKKRFVFISSIATVARIGLAQPGSISEAPVSPSDSACGIGYADGKLVCEKIMEKAAQDYGGQLDVTSVRCGQMTGSKKTGVWNSNEQIPMLLKSAQGLGSLPQLSGELSWIPVDDAASTVSEIAFSDGSMPIVQHLENPIRQSWDAMLQSFGRELGLPAGKVPFGEWLDQVAAADGDDETFPVKKLTFFFKSFFQSVACGQVVLDTTVSRGQSKTLNAMTAVGDETVKAYADYWKSTGYLSK
What is the function of this protein?
Non-canonical non-ribosomal peptide synthetase; part of the asc-1 gene cluster that mediates the biosynthesis of both ascochlorin and ascofuranone, a strong inhibitor of cyanide-insensitive alternative oxidases and a promising drug candidate against African trypanosomiasis (PubMed:30952781, PubMed:35418536).  The first step in the pathway is performed by the non-reducing polyketide synthase ascC that produces orsellinic acid by condensing acetyl-CoA with 3 malonyl-CoA units (PubMed:30952781, PubMed:35418536).  Orsellinic acid is then prenylated by the prenyltransferase ascA to yield ilicicolinic acid B (PubMed:30952781, PubMed:35418536).  Ilicicolinic acid B is further reduced to ilicicolin B by the reductase ascB (PubMed:30952781, PubMed:35418536).  The halogenase ascD then chlorinates ilicicolin B to produce ilicicolin A which is converted to ilicicolin A epoxide by the cytochrome P450 monooxygenase ascE that catalyzes stereoselective epoxidation of the terminal double bond of the prenyl group (PubMed:30952781, PubMed:35418536).  Ilicicolin A epoxide is the last common precursor for the biosynthesis of ascofuranone and ascochlorin (PubMed:30952781, PubMed:35418536).  The terpene cyclase ascF produces a monocyclic terpene, and the cyclization reaction is proposed to be initiated by protonation of the terminal epoxide of ilicicolin A epoxide to generate a monocyclic tertiarycation, which is followed by a series of hydride and methyl shifts with abstraction of proton, leading to the formation of the (14S,15R,19R)-trimethylcyclohexanone ring structure of ilicicolin C, which is finally reduced to ascochlorin by the dehydrogenase ascG (PubMed:30952781).  On the other hand, ilicicolin A epoxide is hydroxylated by the cytochrome P450 monooxygenase ascH, and the resultant product is cyclized by the terpene cyclase ascI to ascofuranol via protonation-initiated epoxide ring opening, which facilitates the 6-endo-tet cyclization to form the tetrahy-drofuran ring (PubMed:30952781, PubMed:35418536).  Finally, ascofuranol is oxidized into ascofuranone by ascJ (PubMed:30952781, PubMed:35418536).
What are the official names of this protein?
Non-canonical non-ribosomal peptide synthetase ascB, Ascofuranone/ascochlorin biosynthesis clusters protein B.
What is the protein family that this protein belongs to?
Belongs to the NRP synthetase family.