Protein: NSRJ_ASPN1
Sequence: MTIPYTPGRLDGKVALVTGSGRGIGAAIAIQLGRLGAKVVVNYANSAEHAEKVVAEIKAHGSDAVAFKADMRRVAETAKLFDDAVAHFGHLDIAVSNSGVVSFGHLKDVTEEEFDRVFSLNTRGQFFVAREAYRVLSEGGRIILTSSNTSKDFSVPRHSVYSGSKGAIDSFVRILSKDCGDKKITVNGVAPGGTVTDMFHAVSHHYIPNGEKFTAEERQQMAAHASPLHRNGFPEDIANVVGFLVSKEGEWVNGKVITLDGGAA
What is the function of this protein?
Short chain dehydrogenase/reductase; part of the gene cluster that mediates the biosynthesis of the tetrahydroxanthone dimer neosartorin, which exhibits antibacterial activity (PubMed:30394754, PubMed:32105084, PubMed:33891392).  The two different monomeric units appear to be synthesized by the same set of enzymes, among which the Baeyer-Villiger monooxygenase nsrF is the key enzyme for the divergence of the biosynthetic routes (PubMed:32105084).  The pathway begins with the synthesis of atrochrysone thioester by the polyketide synthase nsrB (PubMed:32105084).  The atrochrysone carboxyl ACP thioesterase nsrC then breaks the thioester bond and releases the atrochrysone carboxylic acid from AacuL (PubMed:32105084).  Atrochrysone carboxylic acid is decarboxylated by the decarboxylase nsrE, and oxidized by the anthrone oxygenase nsrD to yield emodin (PubMed:32105084).  Emodin is then reduced to emodin hydroquinone by the oxidoreductase nsrR (PubMed:32105084).  A-ring reduction by the short chain dehydrogenase nsrJ, dehydration by the scytalone dehydratase-like protein nsrI and probable spontaneous re-oxidation, results in overall deoxygenation to chrysophanol (PubMed:32105084).  The Baeyer-Villiger monooxygenase nsrF accepts chrysophanol as a substrate to insert one oxygen atom at two different positions to yield the precursors of both monomric units (PubMed:30394754, PubMed:32105084, PubMed:33891392).  NsrF is promiscuous/flexible in interacting with the 2 (non methylated and methylated) aromatic rings of chrysophanol, thus diverging the biosynthetic pathway at this point (PubMed:30394754, PubMed:32105084, PubMed:33891392).  After the hydrolysis of the lactones, methylesterification by the methyltransferase nsrG yields respectively moniliphenone and 2,2',6'-trihydroxy-4-methyl-6-methoxya-cyldiphenylmethanone (PubMed:30394754, PubMed:32105084).  The next steps are the hydroxylation by the FAD-dependent monooxygenase nsrK, followed by isomerization by the monooxygenase nsrQ (PubMed:32105084).  The short chain dehydrogenase/reductase nsrO then catalyzes the C-5 ketoreduction to give the xanthone skeleton of blennolide C and 5-acetylblennolide A (PubMed:32105084).  The acetyltransferase nsrL has a strict substrate specificity and uses only blennolide A but not blennolide C to yield 5-acetylblennolide A as the single-acetylated product (PubMed:30394754).  In the final step of the biosynthesis, the heterodimerization of the 2 xanthones, blennolide C and 5-acetylblennolide A, is catalyzed by the cytochrome P450 monooxygenase nsrP (PubMed:30394754).  NsrP can utilize at least three different xanthones as its substrates to perform the dimerization reaction (PubMed:30394754).
What are the official names of this protein?
Short chain dehydrogenase/reductase nsrJ, SDR nsrJ, Neosartorin biosynthesis cluster protein J.
What is the protein family that this protein belongs to?
Belongs to the short-chain dehydrogenases/reductases (SDR) family.