Protein: ACVA_ACRCH
Sequence: MALEQWKTTVQSVSERCDLSGLSQHPTDYQLASTGVKGAGGSSIEERSAIVSDELFSSLRDVCSQRQLDPRSLMLFSVHQMLKRFGNGSHTVVASLVTSSEGCPSTSAWRAIPSVIHHIEGGDNNNTVASAVEQAANLLNSEGSGQDLLIPIGLTELVKSELIDLLVIFDDETNNIRLPQDFPLILRIHQRQDHWQLSVRYPSPLFDTMVIDSFLSALHNLLSAVTKPSQLVRDIELLPEYQVAQLEKWNNTDGDYPTEKRLHHLFEEAAVRRPQHVALICGDKRITYEELNAMANRLAHHLVSSGIQTEQLVGLFLDKTELMIATILGIWKSGAAHVPIDPGYPDERVKFVLNDTKAQVVIASQRHVDRLRAEAVGGQHLRIIGLESLFDNLAQQTQHSPETSGNLTHLPLNSKQLAYVTYTSGTTGFPKGIYKEHTSVVNSITDLSARYGVAGEDDEVILVFSAYVFEPFVRQMLMALTTGNSLAIISDEDKFDPDTLIPFIQKHKVTYIHATSSVLQEYDFGSCPSLKRMILVGENLTEPRYEALRQRFKSRILNEYGFTESAFVTALNIFEPTSQRKDMSLGRPVRNVKCYILDANLKRVPIGVTGELHIGGLGISRGYMNREELTRQKFLPNPYQTDKERQRGVNSTMYKTGDLARWLPSGEVEYLGRADFQIKLRGIRIEPGEIESTLAMYPGIRASIVVSKKLLSQGQETIQDHLVGYYVCDEGHIPEGDLLSFLEKKLPRYMVPTRLVQLAQIPTNINGKADLRALPAVEVAVAPTHKQDGERGNQLESDLAAIWGNILSVPAQDIGSESNFFRLGGHSIACIQLIARVRQQLGQGITLEEVFQTKTLRAMAALLSEKYTKASNGTNGVTNGTAHVNGHAANGHVSDSYVASSLQQGFVYHSLKNELSEAYTMQSMIHYGVPLKRDIYQAAWQRVQGEHPALRLRFTWEAEVMQIVDPKSELDWRVVDWTDVSSREKQLVALEQLQTEDLAKVYHLDKGPLMRLYLILLPDSKYSCLFSCHHAILDGWSLPLLFNNVHQAYLDLVEGTASPVEQDATYLLGQQYLQSHRDDHLDFWAEQIGRIEERCDMNALLNEASRYKVPLADYDQVREQRQQTISLPWNNSMDAGVREELSSRGITLHSILQTVWHLVLHSYGGGTHTITGTTISGRHLPVPGIERSVGLFINTLPMIFDHTVCQDMTALEAIEHVQGQVNAMNSRGNVELGRMSKNDLKHGLFDTLFVLENYPNLDTEQREKHEEKLKFTIKGGTEKLSYPLAVIAQEDGDSGCSFTLCYAGELFTDESIQALLDTVRDTLSDILGNIHAPIRNMEYLSSNQTAQLDKWNATAFEYPNTTLHAMFESEAQQKPDKVAVVYEDIRLTYRELNSRANALAFYLLSQAAIQPNKLVGLIMDKSEHMITSILAVWKTGGAYVPIDPRYPDQRIQYILEDTAALAVITDSPHIDRLRSITNNRLPVIQSDFALQLPPSPVHPVSNCKPSDLAYIMYTSGTTGNPKGVMVEHHGVVNLCVSLCRLFGLRNTDDEVILSFSNYVFDHFVEQMTDALLNGQTLVVLNDEMRGDKERLYRYIETNRVTYLSGTPSVISMYEFDRFRDHLRRVDCVGEAFSEPVFDKIRETFPGLIINGYGPTEVSITTHKRPYPFPERRTDKSIGCQLDNSTSYVLNDDMKRVPIGAVGELYLGGDGVARGYHNRPDLTADRFPANPFQTEQERLEGRNARLYKTGDLVRWIHNANGDGEIEYLGRNDFQVKIRGQRIELGEIEAVLSSYPGIKQSVVLAKDRKNDGQKYLVGYFVSSAGSLSAQAIRRFMLTSLPDYMVPAQLVPIAKFPVTVSGKLDAKALPVPDDTVEDDIVPPRTEVERILAGIWSELLEIPVDRISIYSDFFSLGGDSLKSTKLSFAATRALGVAVSVRNLFSHPTIEALSQWIIRGSNEVKDVAVVKGGASLDIPLSPAQERLMFIHEFGHSGEDTGAYNVPLQLQLHHDVCLESLEKALRDVVSRHEALRTLITRTQKSSVHCQKILDAEEAQKLFSVDVLRLTSETEMQGRMAESTAHAFKLDEELPIHVRLYQVVRDGRTLSFASIVCHHLAFDAWSWDVFQRDLDAFYAVHTKHKAAANLPTLRVQYKEYAIEHRRALRAEQHRVLADYWLRKLSDMEASYLVPDRPRPAQFDYTGNDLQFSTTPETTAQLKELAKREGSSLYTVVAAAYFLLLYVYTNQRDITIGIPVAHRNHPDFESVVGFFVNLLPLRVNVSQSDIHGLIQAVQKELVDAQIHQDLPFQEITKLLHVQHDPSRHPLLQAVFNWENVPANVHEEQLLQEYKPPSPLPSAAKFDLNVTVKESVNSLNVNFNYPTSLFEEETVQGFMETFHLLLRQLAHNKASTSLSKLSVEDGVLNPEPTNLQPSSRDSGNSLHGLFEDIVASTPDRIAIADGTRSLSYSELNERANQLVHLIISSASIVADDRIALLLDKSIDMVIALLAVWKAGAAYVPLDPTYPSQRTELILEESSARTLITTRKHTPRGGTVANVPSVVLDSPETLACLNQQSKENPTTSTQKPSDLAYVIFTSGTTGKPKGVLVEHQSVVQLRNSLIERYFGETNGSHAVLFLSNYVFDFSLEQLCLSVLGGNKLIIPPEEGLTHEAFYDIGRREKLSYLSGTPSVLQQIELSRLPHLHMVTAAGEEFHASQFEKMRSQFAGQINNAYGITETTVYNIITTFKGDAPFTKALCHGIPGSHVYVLNDRLQRVPFNAVGELYLGGDCLARGYLNQDALTNERFIPNPFYEPKQASDSRPQRLYKTGDLVRFRGPHHLEYLGRKDQQVKLRGFRIELSEVRDAVLAISAVKEAAVIPKYDEDGSDSRRVSAIVCYYTLNAGTVCEASSIRDHLHANLPPYMVPSQIHQLEGSLPVTVNGKLDLNRLSTTQVSQPELYTAPRNSTEETLCQLWASLLGVDHCGIDDDLFARGGDSISSLRLVGDIYRALGRKVTVKDIYLHRSVRALSENVLTDQKDKGTLPASPPLQRAEQGQVEGDAPLLPIQDWFLSKPLDNPAYWNHCFTIRTGALSVEGLRGALKLLQERHDVLRLRLQRRDEGRHVQTFARDCAQPRLTVLDRRSFEDAEDVQEALCEIQSHFDLENGPLYTVAYIHGYEDGSARVWFACHHVMVDTVSWNIILQDLQALYHGDSLGPKSSSVQQWSLAVSDYKMPLSERAHWNVLRKTVAQSFETLPICMGGVLQCQEKFSRETTTALLSKACPALDSGMHEILLMAVGSALQKAAGDVPQVVTIEGHGREDTIDATLDVSRTVGWFTSMYPFEIPKVTDPAQGVVDVKEAMRRVPNRGVGYGPAYGYGGSCLPAVSFNYLGRLDQASSGAQRDWTLVMDEDEYPVGLCTSAEDSGRSSSMVDFTFSISGGQLVMDMSSSWGHGARNEFVRTVRNTLDDLIKTTSSRDFSAPLPPSDQESSFTPYFVFEEGERHGAPLFLLPPGEGGAESYFHNIVKGLPNRNLVVFNNHYREEKTLRTIEALAEYYLSHIRSIQPEGPYHILGWSFGGILGLEAAKRLTGEGHKIATLALIDPYFDIPSASKAIGQPDDACVLDPIYHVYHPSPESFRTVSSLTNHIALFKATETNDQHGNATQQALYEWFATCPLNNLDKFLAADTIKVVPLEGTHFTWVHHPEQVRSMCTMLDEWLG
What is the function of this protein?
Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of penicillin, the world's most important antibiotic (PubMed:1706706, PubMed:2076552, PubMed:9305735).  The trimodular NRPS PCBAB produces the tripeptide N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine (LLD-ACV or ACV) via condensation of the 3 residues L-2-aminoadipate, L-cysteine and L-valine (PubMed:9305735).  The precursor amino acids for penicillin biosynthesis are withdrawn from the vacuolar amino acid pool by the MFS-type transporter penV (By similarity).  Each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates (PubMed:9305735).  The penicillin biosynthesis occurs via 3 enzymatic steps, the first corresponding to the production of the tripeptide N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine (LLD-ACV or ACV) by the NRPS PCBAB.  The tripeptide ACV is then cyclized to isopenicillin N (IPN) by the isopenicillin N synthase PCBCC that forms the beta-lactam nucleus.  Finally, the alpha-aminoadipyl side chain is exchanged for phenylacetic acid by the isopenicillin N acyltransferase PCBDE to yield penicillin in the peroxisomal matrix (By similarity).
What are the official names of this protein?
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase, Delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase, ACV synthetase, ACVS.
What is the protein family that this protein belongs to?
Belongs to the NRP synthetase family.
What are the subcellular locations of this protein?
Cytoplasm, cytosol. Vacuole membrane; Peripheral membrane protein. Note=Loosely attached to the vacuoles.