Protein: MALA_MORAP
Sequence: MNNIDHQNCERTVAADASEYRQGIAKVNSDFDLKAEKSRLFENPELASPVYHPGSAAMSVDESGTPRSASPLSSYTGSPGETRHLGTELSRTLSGDTLSDPKEASSSVQDDVNEKGEDCLFGPSLFDAADAKAGPQSSVGDRFKAQAEYWRTALAGAPALLDLPTDRPRPSHPSFEGDRVPFELNSKTTRALKELSQQQGVTLFLTILSAWSAVLSRLSGQDDIVIGTPSSSRTSEVDSLNGSATKMLALRVDLSGKPNTQELLDRVRRTTLSAFTHQDLSFEQVVEILQPPRKMDHTPLFQVLYSWRNHENSSDLATDAAKFDLTLDMWESDNSIRGSLGYSTALFDRSSIERHVGYLQAMLLEMTTDSELPVALAEILSLDERTFLLETLNVTAENHSDSQHLHQLFEQQAERRPHAIAVVHESESLTYSELNTRANRLAHHLIHVGVQPDSVVAICVERSLALIVGILAILKSGGAYVPLDPVHASERLVGILSDAAPSVLVADACGMKALQGADLSQLKMVDPTSTLPTSAANPRVPNLEMHHLAYVIYTSGTTGKPKGVMVEHRQIARLFATSATQFDFSEQDTWCLLHSAAFDFSIWEMWGALRQGGKLVVVSQDVVRTPHDLHQLLQEHAVTVLNMTPTAFKPLLEIDTANKLQAALRYLFLGGEALSPAMLKPWLLPQAERCPKIINLYGPTEISIYVTYSKVTLEDCSQATSSIGGRLPDMRAYVFDDFMRPMPRGVVGELYVGGPGVARGYLNRPELTAERFLRNPFAAGRDERVYRTGDLVKQLPNGSLLYMGRNDHQVKIRGFRIELGEIETRLVEYPLVSEAVVIATGDDSNRRLVAYVTVRSDEQAPVAESSSTDQLASSLRAHLATQLPEYMVPSAFVRMDAFPMTPNGKLDVQALPAPRESDFARQAYVPPQGELETAIASIWSELLQMQRVSRDDSFFALGGHSLLAVQVISKLHALGHSVSASVLFESPTLSAFAHAIGQHRAIVVPPNLITSDVARITPEMLPLIDLTQTDIDHIVEQVPGGLANIQDIYALAPLQEGILFHHLMHKTGDPYLLYYGRAFDNRALLDQYLVGMQQMVSRHDILRTAFFWEDLSTPAQVVLRSATLSTTELTLDPADGSAIEQLKQRLDPRYNRLDLTQAPLLHYTIAQDFDGRWILGEWLHHLLGDHSTLEHIELEIRAIQEGRGAELLAPHPYRNLIAQTRLGVSQDAHEQFFKAMLSDFDTPSLPFGITDVRGDGSNITESTRMLPADLNERLRFQAKRLGVSLASLCHVAWAQVIARTSGQQRVVFGTVLFGRMQAETSSEGAIGLYINTLPFRVDVDSRSVEESVQETHALLAKLLEHEHAPLALAQRCSDVEPGVPLFSSLLNYRHNMESPEHGSGMEFLGEVERTNYPYDLSIEDYGHSLGLTAQVVLPLVSDRACGYMQEALDSLASALEYNPKMPVSQLDVLPADERKLLTRGGRWTQEDDSYHVCLHELFEQYADRVPNALSVMTDKHSLTYAEMNAESNRLAHRLIDLGAKTDSVMGLCMERSSSMIVAMFGILKSGAAYLPLDPSYTGERLKDIVSDAAPTILVTDAVGRKALGELVTATMVVLDMSDLKGGDTSNPQVPGLHSGNLAYMIYTSGSTGKPKGVMVEHKGVASLAQYHSELIGIHEGSRMLQFASISFDFSVWEIFLTLCSGATLVLAPSSIRMDRNMLWNYMMRQSVTHATFTPSFLQDGVDFPCAIEPLTLTLGGEALGPTLLQNLIQQGIAVFNDYGPTESSISAATWKGSADFNGDVVPIGRPVRNSRLYVLDSQQEPVPLGAVGELYISGVGLARGYLNRPEQTAERFLQNPFGDNKSARMYRTGDLVRYLPDGDLVYLGRTDYQVKIRGFRIELGEIEVRLAEHAMVSEAFVLALGEGINKRLVAYLTTDPEVQIEALDTTSLPSLLRSHLSARLPEYMVPAAFVCLDAFPLTSNGKLDRKALPAPSEDDYARQAYEAPEGEVENAIASIWSELLHVKRVSRHDSFFALGGHSLLAVQVISRLHRLGHSVSVRTLFESPTLLALAQSIGEHRAIVVPPNVITPGVTHITPEMLPLIDLNQNDIDRIVDQIPGGVANIQDIYALSPLQDGILFHNLMHKVGDPYLLYTARAFDNREVLDQYLAATQQIMDRHDILRTAFMWENLSVPAQVVLRNASLPVTELTLDPADGPIVQQLKRRLDPRHNRIDLTQAPLLRFTIAQDSDGRWILGELLHHLTGDHSTLEVIEAELLAIQEGRGAELLAAHPYRNLIAQARLGVSQAAHEKFFKEMLSDFDTPSLPFGIKEVRGDGSNVTESGLTLPSKLNERLRFQAKRLGVSVASLCHVAWAQVIARTSGQQQVVFGTVLFGRMQAETSSDRAIGLYINTLPLRVDVDDRSVEECVQLTHSLLAKLLEHEHASLALAQRCSSVQPGVPLFNSLLNYRHNMDTAQHASSASGMELLESLERTNYPFTISVEDYGTSLGLTAQVAQPLESGRACGYMQEALDSLTTALETSAGMSVSRLNILPTEERQLLTQGGSTQKHDDSYRICLHELFEQHVDRAPDALSIICGDQSLTYADLNAYSNRLAHRLIDTGVKADSIVALCMERSPSMIVAMLAILKAGAAYLPLDPLYPSDRLQGIVTDAEPAVLVADAVGRKALGELSSVHHIEVDIDGLQDGNASNPQVSNVDFTKLAYVIYTSGSTGKPKGVMLEHQGVSSLVQYHRELLRVCEGSRMLQFASISFDFSVWEIFIILCSGATLVLAPSSMRMDRDMLWRYMDEVSVSHAACTPSFLQDGADLPPLTSPLTLALGGEALSPALLQNLIRQGINVFNLYGPTETSIATATWNCPTNFCGDIVPIGGPVRNARHYILDSQQLPVPMGAIGELFIGGIGLARGYLNRPEQTAERFLKDPFSEDEGARVYRTGDLVRQLPDGNLVYLGRADFQVKIRGFRIELGEIEARLVEHESVSEAVVVALGEGVEARLVAYFTRDHDGKSEAFDKSLLPSILRSHLSARLPDYMVPSAFVRMEAFPLLASGKLDRRSLPAPSEEDYARQAYEAPQGAVEAAIASVWSELLNVNRVSRHDSFFALGGHSLLAVQVISRLHGLGHSVSVRALFESPTLSALAQSIGEHRAIVVPSNVITLDSIRITPEMLPLIDLTQDDIDHIVEQVPGGVANIQDIYALAPLQDGILFHHLMHKVGDPYILYSATAFDNRASVDQYLAATQQIVDRHDILRTAFMWENLSTPTQVVLRNAPLSITELFLDTSKGPVVQQLQKRSDPRFHRMDLTQAPLLRFTVAQEDDGRWIVSQLLHHLIGDHSTLEVIELEMKAIKEGRAADLLAPHPYRNLIAQARLGVTQDAHEKFFKEMLSDFDTPSLPFGITDVRGDGSNVTESERDLSAELNERLRFQAKRLGVSLASLCHVAWAQVIARTSGQQQVVFGTVLFGRMQAETSSGQAMGLYINTLPLRVDVDNRSVEESVQQTHSRLAKLLEHEHASLTLAQRCSDVPPGAPLFSSLLNYRHNAEMPERGSNISGKEEVDSRERTNYPFSISVEDYGTALGLTAQTVQPLDSDRACGYMQEALDSLASALEHNPKMPVAHLNVLPAEERTLLLDTWNDTAEDYPEHLCLHQLFEQQAERTPDTLAVVCEDKSLTYRELEARSNGLAHHLIQLGVRTDDIVAICVKRSVEMIVGILAVLKAGGAYVPLDPFFASDRLKDIMSDAAPVVLLADEAGRTALGKSAVSDVVVVDPTLFTTKADSPPRVADLSSQNLAYVIYTSGTTGKPKGVMVEHQGVVNLVVSQQPLLNISTSSRFTQFLSISFDPSVWETFATLSFGGVLHVLQEDVRRDFRQLWAYLQLNQITHAIFTPAVIQDCEGLPPLESMSTLLIGGEALSGALVRRVGDLVPNAAIINEYGPTEASIAALSWTYVEGGLIGDDIVPIGHPLSSKRVYILDEFGLPLPLGVAGELYLGGVGVARGYLNRPDLSAEKFVMDPFSREPGARMYKTGDMAKYHTDGRVICLGRNDDQIKIRGFRVELGEIEARLVEYPQVSEAAVVPLGKGGNMRLVAYVIARDEGEVEQHSDRSQSASSTELATSLRTHLLAKLPDYMVPSAFVRLSSFPVTTNGKLDRRALPAPNEDDYAREAYEAPQGEVETALASIWCELLQLKRVSRNDSFFALGGHSLLAVRLVNRVSTLGATVAISTLFASPSLSAFASRVQEQLAQEESSVMAITPVSRDNDLPLSFAQQRLWFLAQLGGVSDTYHMPLALRLQGQVNQVALESSLSELCNRHEALRSVFVTVNGQAHVKILPPGGLSIRKVDLRGAADQEIQLRQWMDKETHASFDLEQGPLVRASLIQTQDDECVLLITQHHIVSDGWSVGIMLRELSQLYTAFCSGESSPLSPLRIQYPDYAAWQRKWLSGDQLKSQSEYWRTALSGAPVLLDLPTDHPRPAHQSFKGDRVTIAWDADITRAVKQLSQRHGVTLFMTILSAWSAVLSHLSGQDDVVIGTPNANRNHPEIESLIGFFVNTLALRIDLSGKPTTQELLERVRRSTLAALNHQDLPFEQVVEAVQPPRKMDHTPLFQVMFAWQNNEDDDLELPGLQVTPCDLDYDAAKFDLTLSLWEEDSGIGGNLEYSTALFDRTTIERHVGYVHAMLLAMTTDDEQPVAAAEILSQDERTLLLETLNVTAESQSDKSCLHQLFEQRVESTPDAIAVVHDEQTLTYGELNARANRLAHHLIQLGVKPDSLVAICVDRSLPMLVGVLAILKAGGAYVPLDPVHASSRLLDILDDVQSSVVLVDARGTKALQGSDLSHMNVVDLREPLSGPTHNPQVMDLSSNHLAYVIYTSGSTGKPKGVMVEHRQVARLFTATSVWFDISEQDTWCLLHSFAFDFSVWEIWGAFLYGGKLVVVSQDIARSPQELYRTICEQAVTVLNMTPSAFKQLIDIHSGEQLDDSLRYVVFGGEALAPAILKPWFHTHAQDRPKVVNMYGITETTVHVTYRLMTPEDCSQTTSPIGVRIPDLRTYVLNDCGRPVPLGVMGELFVGGAGVTRGYLNRPDLTADRFIPDPFVEGHEGRMYKTGDLVKQLPDGSLVYMGRNDHQVKIRGFRIELGEIEARLTDHARVIEAVVIPLGDERNKRLVAYVSIREGEMSEEDGDDAESSFAERLASTLRTHLSTRLPEYMIPSAFVHMASFPLTPSGKLDMRALPAPSEDNVARQAYEAPEGEVETAIASIWSELLQIERVSRHDSFFALGGHSLLAVQVISKLHRVGHSVSVRALFEAPTLAVFAASIGHHQAIVIPPNVITPSTTSITPEMLPLIDLTQTDIDHVVEHVPGGVSNIQDIYSLSPLQDGILFHHLMAKSGDPYLLYVAMRFDTREALDQHLAGMQLIVNRHDILRTAFMWENLSSPAQVVWRNAPITVMEMNLDPSEGPILQQMKERFDPLHYKIDLTQAPVLRFAVAQDIDNQWILVRLLHHLVEDNSTLKVLHSELQMFAENSNAVLPPAEPYRNLIAQARLGMSQQAHEKFFKAMLEDIDTPSLPFGMANVHGEGADVIVSDRMLPQSLNDRLRLQAKRLGVTVASLCHVAWAQVIARTSGQQRVVFGTVLFGRMQAETSADQAMGLYINTLPIRVDIDGRSVEDSVQQTHSLLAKLLEHEHAPLTLAQRCSGIPAGGPLFSSLLNYRHSEDDDEEESGEEDMELLDFQERINYPFGISVEDLGTSLGLTAQVAAPLDSYRVCGYMQEAMESLANALESNPKMAVAQLDVLPAEERTLLLETWNNSAEEYPDSLCLHHMFEQQSERTPEAVAVVYGDRSLTYGELNARANGLARHLVHYGIQPDERVAICVKRSPEMLVGIMAILKVGGAYVPLDPLFASDRLKDIINDAAPRILLADEAGRAALGKSIVSGLTAVDPTLFGADLGASPRVAGLSCRNLAYVIYTSGTTGKPKGVLVEHQGVVNLLTSRQKHQLVQPSSNLTQFFSYSFDASILEIFGSLSFGGTLHILQEDVRLDFHQLWTYMEKHEITHAALTPAVLQNCEGLSPLVSMSTLIIGGESLSEGMVRKVSELMPNAAVVNEYGPTEATVATLSWKYSNEGMIGHDLVPVGRPLSNKRVYLLDDQSRPVPLGAVGELYIGGAGIARGYLNRPDLTAEKFLVDPFASESGATMYRTGDLAKYHPDGNVICLGRNDDQVKIRGFRVELGEIEARLSEHPQVSEAVVVPLGEGSLLRLVAYIIARTEDVLEQNIDTAEPMQLSSSLRSHLATRLPDYMVPSAFVCLSALPLTSNGKLDRRALPAPSDDDYAREAYEAPQGEVETALALIWCELLQLKRVSRNDSFFALGGHSLLAVQVISRLHRLGHSVSVRALFDSPTLSALAQSIGQHHAIVVPPNYITPGVTRITPEMLPLIDLSQMDIDHIAERVPGGVANIQDIYALSPLQDGILFHHLMQKSGDPYLFFTARSFDTRLSLDMHLSAMQQIVDRHDILRTSFVWEGLSAPAQVVWRTAPLSITELDLDPAQGSAVQQLQERFDPRNNRIDLSQAPLLRLAVAQELDGRWILMELLHHTIGDHSTLETTEIELREIQEGRGADLLVPHPYRNLIAQARLGVSQEAHEKFFKEMLEDFDTPSLPFGIADIFGDGSQVTECHRMLPQSLNDRLRAQAKRLGVSVASMCHVAWAQVIAQTSGQQRVVFGTVLFGRMQAETSSDQAMGLYINTLPFRVDVNNATVEEGVHQAQSLLAKLLEHEHASLTLAQSCSGIPAGSPLFSSLLNYRHNSAELLEEASNNSEMDILNSQERTNYPLGLSVEDFGTSLGLTAQAVLPLDPSRICGYMQEALDNLASALEFNPTMTVAQLDTVPAEEHNLLLQAFNDTAEEHPSSLCLHRMFEHQVERTPAAIAVVHEDQSLTYSELNVRANRLAHHLIQLGVQVETLVAICVKRSPEMLIAILAVLKAGGAYVPLDPLYASDRLREMVSDAAPSVLIADDAGRKALGGPFVATLTVVDPSTAFAGDCSDPHVGGLTSNSLAYVIYTSGTSGTPKGVMVEHQGVVSLVSSRQKQLLVEPTSRMTLFFSVSFDPSLLEIFGTLGFGGALHILGDHIRQDRHLLWKYLEHHRITHAILTPAMLQEFDDSSSLTDMQTLLIGGEALSVRLARKARKIVSNGAVINEYGPTEASIAALSWRYAEHVLHEHTPIGRPFSNRRVYLLDAEGTPVPLGAIGEIYLGGLGVARGYLNRPDLTAERFVADPFSGVSGARMYKTGDLGKFLPDGNVVCLGRNDHQIKIRGYRIELGEIETKLAEHEMVSEVVVVTSGSDADRQLVAYVVANHEHQMTREVDAADSSSLAQLAATLRSYLSARLPDYMVPAAFVRLDALPLTPNGKLDRHALPAPLSHAFATEDYEEPRGAIENILAGIWAELLNIDRVGRNDGFFVLGGYSLLAVRMISRVRAMLGLDLSLRTLFEAPTIAELAPRLLATGVTQDESYDVLLPIKPQGSRPPLFCVHPVTGLSWCFTGLSAHLDSDQPLYGLQARGLIDNGNMASSLDEMVVDYIDQIRRVQPHGPYYLLGYSFGGLVAHTMAAYLEKQGEQVALVALMDTPADYRTMAPEDKDEKQREQSLIQAFSKNRDLYSPEMINPVLRKALQTVNINNNKLGRLQAPRVTGGDLLIFRATVMEDEGRALLDPNAWKPYVQGSIEVCDVDCAHDFMDTPEATVVVSQVLNQKLRQSHCCLQREE
What is the function of this protein?
Heptamodular nonribosomal peptide synthetase that catalyzes the biosynthesis of malpinins, natural products that show biosurfactant activities (PubMed:36091214).  Malpinins are acetylated hexapeptides (Ac-D-Leu/Val-D-Arg-D-Leu/Val-L-Phe/Leu-Dhb-D-Trp) containing a non-canonical amino acid derived from dehydration of L-Trp, (Z)-dehydrobutyrine (Dhb), at position 5, as well as a C-terminal D-amino acid, D-tryptophan, that can be oxidized to kynurenine (PubMed:36091214).  Incorporated D-amino acids in positions 1, 3 and 4 are variable resulting in the malpinin A-congeners malpinin B to E (PubMed:36091214).  Both modules M1 and M3 have relaxed specifcity towards aliphatic amino acids (L-Leu > L-Met > L/D-Val > L-Cys), explaining Val at position 1 and 3 in malpinin A-congeners malpinin B to D (PubMed:36091214).  The incorporation of L-Leu, but not N-acetyl-L-Leu by module 1 suggests the N-terminal acetylation occurs at a later stage of biosynthesis (PubMed:36091214).  Similar to M1 and M3, M4 has a broad substrate spectrum showing the highest activity with L-Phe followed by other hydrophobic amino acids (L-Phe > L-Met = L-Trp) (PubMed:36091214).  In contrast, M2, M5 and M6 are highly specific for L-Arg, L-Thr, and L-Trp, respectively (PubMed:36091214).  Solely, M7 converted its preferred substrate (L-Phe) with a 15 000-fold reduced turnover rate compared to the most active module M6, indicating that its A domain cannot contribute to the malpinin biosynthesis due to low activity (PubMed:36091214).  Since the last T domain in malA is apparently not loaded with an amino acid, either the TE domain must offload the oligopeptide from the preceding T domain or the dual E/C domain of M7 must transfer the final peptide chain to the free acceptor T domain of M7 prior to release (PubMed:36091214).
What are the official names of this protein?
Malpinin synthetase, Nonribosomal peptide synthetase 3.
What is the protein family that this protein belongs to?
Belongs to the NRP synthetase family.