Protein: FLVI_ASPFN
Sequence: MAKDQSMSRTASLCQEITVPKTIPVCIQDVFYRRVLEQHDAPAVCAWDGELTYGELDDKSSSLARILAQKGIHRGSFVPLCFDRSLWTAVAMLAVLKAGGVFCFLEPKYPLARLEHMCRHINAKMVLSGESRSELARKLGEHLAVNEDLLATSPSDQELVDVAPNQAAYVAFTSGSTGKPKGILVSHQALVAGILYNDKPMYLNRTSRVLSFASFAFDVSFLEHFWALLVGGCMCIPSESDRENNLLEAIENLQVNWAFLTPSVARVLNPTKLPSLRHLIMGGEPITQTDIDMWSPHVHLIGVYGPAECAGCTTIQSDYGKVESAANIGFPYAVTCWIVDENNHNVLVPTGSIGELVVQGPSLSEGYVNDPEQSAKSYITNPLWLSGSKEAEQQLYKTGDLVRRLSDGSLHFISRKDTQVKINGQRIELGEVEHHTRAVLGGNREVIVEAVKAGRPSSSLVAFIVTDNIPQSSTELFLAPDAGFKDRINTTKSLLRERLPDYMVPETYISINHLPSTVTGKADRKRLREQFTLLTRAQIKAYFGLEDKVKVMPLTEIELKMQRLWAKVLNLDLHEIARDDDWVSLGGDSLGAMQLASLARSEQFFLTVPEIFRHKTISMLCQNIKTDVSETIEEMKPFALLCDHELESDRILQTIADQCQVSQNSIEDAYPITSLQLDASIIPIQWGLNYTLRLEFKLPPTVDPAQLTLAWEMTVASNPVLRTRIVELTKDHYIQAVIRSKIPLENLDSSNMARYEPAVDVWGIGKPLVRVGLQANRFVMLIHHAIYDGQSLPLVFRDISNAYQGQKLALIHFAPFVRWSKSLDAPKRQFWIDKFAGFDGRVFPPVLDPSLDPVESRELTGHLNIVHDAFTATNKIRVTLAIVISWHHGTNDVVFGGVFARRSAPIPDIIDSAVPTTAMLPDRIKLDPDETLRYNLERDQDNILSLMLHEGIDDRDIEQLSPECEAVACKYGTLLAVQPDLATAYPEMFRERDMQYYGPICALNALMQCYLSPESATISLRLSESTMDGVYHWGRFLDEFEAVFHFIQKNPDVKLCYLRRQLDIPNPRSPA
What is the function of this protein?
Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of flavunoidine, an alkaloidal terpenoid with a tetracyclic cage-like core connected to dimethylcadaverine via a C-N bond and acylated with 5,5-dimethyl-L-pipecolate (PubMed:31885262).  The tetracyclic core is synthesized by the terpene cyclase flvE and the cytochrome P450 monooxygenase flvD (PubMed:31885262).  The terpene cyclase flvE catalyzes the cyclization of farnesyl pyrophosphate (FPP) to form (1R,4R,5S)-(+)-acoradiene and the cytochrome P450 monooxygenase flvD is then responsible for oxidative conversion of (1R,4R,5S)-(+)-acoradiene into the tetracyclic cage present in the final product flavunoidine (PubMed:31885262).  In parallel, the N-methyltransferase flvH dimethylates L-lysine to give N,N-dimethyl-L-Lysin which is decarboxylated by flvG to afford dimethylcadaverine (PubMed:31885262).  The terpene cyclase-like protein flvF is the enzyme that attaches the dimethylcadaverine precusor at the C-7 of the tetracyclic cage to yield pre-flavunoidine (PubMed:31885262).  The cytochrome monooxygenase flvC hydroxylates the C-10 position of pre-flavunoidine whereas the NRPS flvI acylates the terpenoid core at the hydroxylated C-10 with dimethylpipecolate to yield final flavunoidine (PubMed:31885262).  The bifunctional enzyme flvA and the dehydrogenase flvB are responsible for the synthesis of the dimethylpipecolate precursor (PubMed:31885262).  The PLP-dependent lyase domain of flvA might use L-O-acetyl-homoserine and alpha-keto-isovalerate to form an intermediary ketone that can cyclize intramolecularly to yield an imine (PubMed:31885262).  The imine can be reduced by flvB to yield the 6-carboxylated pipecolate (PubMed:31885262).  The C-terminal alpha-KG-dependent oxygenase domain of flvA is then proposed to catalyze the decarboxylation to yield dimethylpipecolate (PubMed:31885262).
What are the official names of this protein?
Nonribosomal peptide synthetase flvI, Flavunoidine biosynthesis cluster protein I.
What is the protein family that this protein belongs to?
Belongs to the NRP synthetase family.