Protein: AZPA3_ASPTN
Sequence: MALLEDIINFATLNPMVVVAIPVFLFVISLLRSYLRLAHIPGPFAAGWTNLPRFSWVLSFKAHDIHTALHRKYGPLVRFGPNMVSVGDPKEVGHIYGFTDPWMKSDFYHALLMKPRGKPIPGIFAAQDENIHRALKKPISSAYSMSTLLSFEPYVDSTMRVFCEQLESRFIENKKPLDFGKWLQMFAFDVIGELTFSRRLGFLESGEDINHVMANIWETFKKTSLVTQMPWLDKLWTNNPIQRWRRGGGASPGAAFAMARVEERRELQRTTNKNDWHFNTRDFLSRFMEAEAKDPSIPPYALAAWASSNITAGSDTTGIFLRTIFYQLLTHPETLRKLREELDQAAAAGNLDDLASWKQTRELPYLDAVIKEGGRIHPPFGLPYERVVPAQGATICGKFLPGGTLVGMSAWVVHRNKELYGEDCDEWNPDRWLKCDTEKRRKMENALLTFGAGHRTCMGKHIAYLEMYKVVPTLLRKYDVSRLLCFLVSRTG
What is the function of this protein?
Cytochrome P450 monooxygenase; part of the cluster A that mediates the biosynthesis of azasperpyranones, members of the azaphilone family that exhibit anti-cancer activities (PubMed:31908094).  Azasperpyranones are synthesized by 2 clusters, A and B (PubMed:31908094).  Cluster A is responsible for the production of the polyhydric phenol moiety while the azaphilonoid scaffold is produced by the cluster B (PubMed:31908094).  The non-reducing polyketide synthase ATEG_03629 produces 5-methyl orsellinic acid, which is then reduced to 5-methyl orsellinic aldehyde by the NRPS-like protein ATEG_03630 (PubMed:24412543).  5-methyl orsellinic aldehyde is then first hydroxylated by the FAD-dependent monooxygenase ATEG_03635 and subsequently hydroxylated by the cytochrome P450 monooxygenase ATEG_03631 to produce the unstable polyhydric phenol precursor of azasperpyranones (PubMed:31908094).  On the other hand, the polyketide synthase ATEG_07659 is responsible for producing the 3,5-dimethyloctadienone moiety from acetyl-CoA, three malonyl-CoA, and two S-adenosyl methionines (SAM) (Probable).  The 3,5-dimethyloctadienone moiety is then loaded onto the SAT domain of ATEG_07661 and extended with four malonyl-CoA and one SAM, which leads to the formation of 2,4-dihydroxy-6-(5,7-dimethyl-2-oxo-trans-3-trans-5-nonadienyl)-3-methylbenzaldehyde (compound 8) after reductive release and aldol condensation (Probable).  The FAD-dependent monooxygenase ATEG_07662 is the next enzyme in the biosynthesis sequence and hydroxylates the side chain at the benzylic position of compound 8 (Probable).  In Aspergillus nidulans, afoF, the ortholog of the FAD-dependent oxygenase ATEG_07660, is the key enzyme for the biosynthesis of asperfuranone by catalyzing the hydroxylation at C-8 of to prevent the formation of a six-membered ring hemiacetal intermediate and thus facilitatings the formation of a five-membered ring to produce asperfuranone (Probable).  In Aspergillus terreus, ATEG_07660 is probably not functional, which leads to the formation of the six-membered ring hemiacetal intermediate presperpyranone instead of asperfuranone (Probable).  Finally, ATEG_03636 is involved in the condensation of the polyhydric phenol moiety produced by cluster A and the perasperpyranone precursor produced by cluster B, to yield azasperpyranone A (Probable).  Further modifications of azasperpyranone A result in the production of derivatives, including azasperpyranone B to F (PubMed:31908094).
What are the official names of this protein?
Cytochrome P450 monooxygenase ATEG_03631, Azasperpyranone A biosynthesis cluster A protein ATEG_03631.
What is the protein family that this protein belongs to?
Belongs to the cytochrome P450 family.
What are the subcellular locations of this protein?
Membrane; Single-pass membrane protein.