Protein: PYNA_ASPNC
Sequence: MDTPLSSSEISPRFSNTVPSSVSSMTPNADPSVIVGLACRVPGATNPSQLWENIVAQKDLQRKMPADRFNVDAFYHPDGTNKGTTNAKFGYFLDQDIGMFDAGFFRISGKEAEAMDPQQRLLLEVVYEALEDAGITLDEVNGSNTAVFCGSFTNDYNAMVTKDLEYYPKYTVTGTGNAILSNRISYFYNLHGPSVTIDTACSSSLNESDIAIVVGSALHFDPNVFITMTDLGMLSSDGRCRTFDSMGSGYVRGEGICAAVLKRRRDAVYNGDNIRAVVRASGVNHDGIKQGITLPNTNAQEKLIRRTYDLAGLDPNDTQYFEAHGTGTARGDPIEARAIGAVFGSTRSEPLYVGSVKSNIGHLEGASGLAGIIKATLALEESQIPPNMHFKRPNPEIKFDEWKIQVPQDIISWPASANGIRRASINSFGYGGTNAHVILDAYKPEDSEAELQAIPAISSSIPVDRPYLIPLSAHSTKAGALWEDKLTKYLSNEPIGRPAVSDLAVSLSTRRTMHGNRSFIIGKDMPTVLQGLEQPPSPAAAWTRPLKETPRLGFVFTGQGAQWFAMGRKLIQQSHLYRQTLERCDAVLQSLPDGPDWTVLEELLRTEEASRLKETRLSQPICTAMQLATVCLLKQWGIEPSAVVGHSSGEVAAAYAAGILTFENAMIAAYYRGLYMSSGVDGSMTTDGAMMAVGLTEAEAKKEFETYTGQICVAAVNSASSLTLSGDKDAIVRLRDSLVERKIFARLLQVAQAFHSHHMLPLAPKYEEALKNCAGFGTSPARVRMFSSVTARLARPGEMGAGYWTANMTGTVRFSDALTGILLNEEDEQNVDILVEIGPHPALKGPSRQVMNALKLNLPYLASLTRGVNDYESLLTLAGQLFQYGFPVDLIAVNSDHFLQRETGMIQSELHGKRLRDLPTYAWDHKRYWSETRPIREHRLRKQRHSILGARMPGIPERTPHWRNYLRLKEIPWLADHVIDGNAVFPAAGYFSMAIEAAVSMCAEDSVIKEIALRDLNVQSALLLSDSEEGTEVIMELRPATQSAKSKSALWYEFTIYSYGESKILNEHCSGLVSVETNALTLPMRWESSKTFDDLAKESQESIPAETLYDHLTALGLQYGPSFQLLTGDVQTGPGFALAGLDFQPSQFSVQAADLTIAHPTLLDASFHAIFPAIESALGRSLDEPLVPTFVRSFKVSGDFLACCRESREQKFQVTCFTRLPGPRVALSDLTVCSKESNKPLLQFNGLEVTALGSDKTDNSAGRSLFFRTRWQPAFTFLGPDHPAVAQKNISEILDIFAHQFPDTRILHVSDTVDGTRDVLKYLGGRSNERRRFHSITPVFQTQIALEEIDALSQEWPGLVEISEPEPNAYGLVVLSSDAADLDSRQFVKEGGFVLALGPHPQPEGLHDVFFTKDLAVWQKSTDNAQKPKQLSLILPSCPSQRTLDIADGMEMQHGSSVFVTRTSLAALSNEALQAEDIVVLANLDEDVLFEHSSSDQSTFLGIKRLLTAGGKNIVWVLEGGSMDAPKPEHAMIIGLARVARSENDQLRFVTLDLPRASTQETVVRHVWRLLDRSITEDEVAVRDNCIFIPRVEADDQLNSKLRNGTNSQPREEPLGAGRPLALKIGRVGLLETLVFEDDEQILDTQLADDEIEIEVKASAINFRDIAASMGIIDDYKLGDECAGIVTRIGAQVNPQDFQVGDRVAAWRPGQGAHKTIVRNPASLSYKLGDMSFVDAASLPCILTTAYYSLVHVAHLQPGETVLIHSAAGGVGQMAIQVAQYVGARVIATVGSQAKRSLLKSRYGLADDMIFNSRDDSFVRDVLDTTGGRGVDVILNSLAGKLLHATWSCVAPFGRFIEIGKRDIHENSKIDMDPFRRNVAFASVDLVTIFEKNKPLGARLLKECGTLVHKGHITPPETVTELPYSDAVKAFRLLQMGKHTGKVVLVPHAGDRVPVLPSTYRNQPLFKHEKTYLLVGGLGGLGRTLAEWMVRKNARRLAFLSRSGADKEEAKRTVEWLRERGVSVTVFKGDVSRYEDVERAVKAIDNLGGIFQAAMVLQDAPLENMSYQQWQICVEPKVKGTYNLHQATLGKQLDFFICFSSASGSIGSKGQANYSSANCYLDALMRHRREMGLAGTTMNCGMIVGIGAVAANQALLKVMMRSGYDGVNKEELLYQIEEAVLSDNNKKVSRRGVDLHQTITGINMTKADFYWCQKPLYRNLYNNHEFLGQTAIKQGTKSLASQLQGTKSVEERTTLVLSAFIEKVADVLSVSVDSIESANPLSAYGLDSIIAVEFRKWFSRSVGVEIALFDVLGAPSIFALVTKASGLITITTSNDDKAENVDNEGAKGNEDQEVETQQGQLNQPIPPAAAVGPVPMSSFQQRLWFIHNFGDDKTFLNLSITSYLEGNPDATILEKALNELVNRNAILRTGYTEGDEFAEQTVLDMPSISLERIDVSSKPSPTVSLQDVIQRRRAIELEIEEGEVVRPMLVRLSDDQHALVLICHHIAIDRGSAKSSLNQLTGIYDAIRQGRDLDMVPRPGVSYADFAVWHNRLLSSPSLQADLTFWKENLSGMPKTCKLLPFAKSERPLHDDLQRTVVSGILKKSLLNRMKRICSQSGATPFQFLLAAFRAFIFRYTEDSDLGILMIDGDRPHPDLEDVLGFFVNMTPIRCQDSCEGAFDQLLEATKTRTLEAMSHSKAPFDSIVDVVKAKKTTSHFPLAQIALNYQIHGTFPVYRTQDFNVHDVQSVDVPTACDMQLEALEHPERGLDLRLEYSSTLYGSGDMNRFFDNFVTFMSSLIRDHRQPIAEVNLCGALEIAHLEKNFWNTQFTQNPWGSVGVCQRIMENAAKQPEAVAIAASDGAAITYSELVERAQRVAASLKASGVTERQKICVLVDPGVDAVIALLAVLLTRSCYVALDSSFAVDRLAFMASDCGAGVLLFGPELQGLAETVASKSKSGLRLLDTKKAALCEDRFVGDLPSVNEDPFFIIYTSGSTGKPKGVVLSHANTQQMLASVGEYFRFTSDDRFLQQSSLCFDLSVVQIFSALTAGARVCVAKHDIRKDPAALAAFMHETGVTITYFTPTHFALLLEHSWETLHQCSQYRAALFAGERLPVRIARAFYDLQTPAVVYNTWSPSELVVQTTIHKVDKPDDDVFDIPIGRPLPNCRHYVVDAVLNPLPAGFVGEICVGGAQVGLEYLNRPLANATSFVRDLNSTPEDQARGWKKMFRTGDKGSFLPNGLLTFKGRIAGDKQIKLRGFRIDLGEVEQVLYKNASTPDGQGIVDISVIARDSEKSDATSPLTDERRLIAFVIPKKPLQSTQERDEYANYLHRMAQGSLNEYMCPNGYQFLERLPMTIGGKVDRRSLLTMKLDLAQHTTTCTDSRPVTEVAGDDAEILQGVTGQVCSLLGIDRSIAPNDNFFELGGQSILLLRLQSRLKKKFKVTLKLQELIHAPTPLAIAGMIQKQLKGPAGVQNENASKSIDWSEEISLPASLMNTDYSQLSRFPRTDVSSILLTGIDTFIGLHMLATILSNNHNATVYVIGIHDELTADHLVEGLTKYKLLDAHLSTEDVLSRTCAVPGKMTSPRFGLAEEAFRNLADKVRVIFNIAADVSLLKTYVDLKTVNTSAILTLIELATSSHGHLLEIHHLSTWSVPHLQTWKKTSRTRAFASNREEDPSHFTPPTADEYGYFKSRWAAEMYLTQAAARGVPVSIYRASSVSGSRATNVPVPEMDFISNMIMHMIQHRAIPEINSSSLIDEAGDFVVDFLPVDALTSSMYTLASEESAAAPGLQVYHLGSSQPLPLQALVDVIPSLDQSGAAGKCRVVPMQEWLRLVSEGASEEEQLHWMVVKKYFQHGHSMFALDKSHTVAALKKAGKEVEFPAIDVDYLKRLLDERGPGLKR
What is the function of this protein?
Hybrid PKS-NRPS synthetase; part of the gene cluster that mediates the biosynthesis of pyranonigrins, a family of antioxidative compounds (PubMed:24106156, PubMed:26414728).  The first step of pyranonigrins biosynthesis is performed by the hybrid PKS-NRPS synthetase that condenses 6 malonyl-CoA units to an acetyl starter unit, to form a 1,3,5-trioxotetradecane-6,8-dienyl-ACP (PubMed:24106156).  The enoyl reducrase (ER) domain of pynA is likely to be functional during the first two rounds of polyketide chain extension, to generate the saturated C-C bonds of the alkyl side chain (Probable).  PynA subsequently forms the amide bond between the acyl chain and L-serine (PubMed:24106156, PubMed:26414728).  Although pynA has a terminal reductase domain, it appears to require the thioesterase pynI for the release of the straight-chain intermediate from pynA via the formation of a tetramic acid pyranonigrin J (PubMed:26414728).  The methyltransferase pynC then coverts pyranonigrin J to pyranonigrin I via N-methylation (PubMed:26414728).  The FAD-dependent monooxygenase pynG catalyzes an epoxidation-mediated cyclization to form the dihydro-gamma-pyrone moiety, followed by pynD-catalyzed oxidation of the alcohol to the ketone and enolization to yield the characteristic tetramic acid-fused gamma-pyrone core of pyranonigrin H (PubMed:26414728).  Pyranonigrin H is substrate of pynH for dehydration-mediated exo-methylene formation from the serine side chain to produce pyranonigrin E, before the oxidase pynE reduces the exo-methylene of pyranonigrin E into a pendant methyl to form pyranonigrin G (PubMed:26414728).  The FAD-linked oxidoreductase pynB performs the reverse reaction and converts pyranonigrin G back to pyranonigrin E (PubMed:26414728).
What are the official names of this protein?
Hybrid PKS-NRPS synthetase pynA, Pyranonigrin biosynthesis cluster protein A.
What is the protein family that this protein belongs to?
In the C-terminal section; belongs to the NRP synthetase family.