Protein: PKS8_PESMI
Sequence: MRPTTPSTTESPDNFSNMKLLYFSNEFPHDDLAGLSRQLTLLSKQKQFPHLARFLDHATEAVRDEVRQLPWSLRNTIPSFDSVFNFIEHASLRKGELGASIDGVLLVVVQLGTLIKYYEENPKKYDLNTGNAALSGLGLGLLSTAAVSLSSSVSDLAVAGVEVVRLAFRLGVVVGSVSSNLESRDPTSPPVSWAAVVPDVTVDEARDELDAFYAREKMPSTSKIFISAWSHNSVTISGPPSRLKHLFRTSEFFRGQKIINLPVYGGLCHAAHLYNQEHVREVVSETDSLKELSSRASPKLTTFATSTGKRFEAETARELFEQIVAEIMTKEIRWGTVVDSVLAQAHTTSCSDIQVLVFRKSLPVYDLLTAFSSKQPGLEVSTLELIPTIPASLDNGTPVSGGSSKSSIAIVGMSCRMPGGATDTESFWNLLEKGMDVHRTIPADRFDIDTHHDPKGKAMNSSHTAYGCFIDEPGLFDAPFFNMSPREAQQTDPMQRLALVTAYEALERAGYVANRTPATSLERIGTFYGQASDDYREVNTAQEISTYFIPGGCRAFGPGRINYFFKFAGPSFSIDTACSSSLATIQTACTSLWNKDTDMAVAGGMNVLTNSDAFAGLSHGHFLTKTPNACKTWDSEADGYCRADGIGSIVMKRLEDAIADNDNIIGVIRGAATNHSAEAVSITHPHAGAQSFLSSKVLSAAGIDPFDVSYVEMHGTGTQAGDKEEMKSVTDVFAPAGPKRRSMRQPLHVGAVKANVGHGEAVAGVTALIKVLLMFQKSMIPPHVGIKNSLNPALPSDLAERNVRIPYTRQAWPREKDRKRIAAVNNFSAAGGNTMIAIEEPPLPRNIAPEDEDPRTTHAVTVSAKSKVSLEGNLKRLIAFLENNPDVRMSDLAYTTAARKYHHNHRIAVAAADVPQLKKKLSAQLNSIDSVKSISNLQPLIAFAFTGQGASYKSMDLQLFHHEPTFRSQILHLNSLALAQGFPSFLPAVDGSFPKDHAHSAVVTQMALVAIEISLAAYWASLGVVPNVVVGHSLGEYAALYVAGVLSASDAIYLVGQRARLLEERVQAGSHKMMAVRASLADIQKIAAGMPYEVACINGPRDTVLSGLSKDLDTLIEPLQKAGFKCYSLDVAYAFHSSQTDPILEEFGELAETGAIFHAPKLPVLSPLLGKVIFDEKTLNATYMKRATRECVNYLAAVETGAKLSLVDDNTLWVEIGPHPVCINMSKSSLRSVGAAVPSMRRDESNWATMAQSLSTLHCAGATIRWNEFHKPFEKGLRLLDLPTYAWNNKNYWIQYNGDWALTKGNTYYDAQKKALETAKALPAPRLSDLKTSSVQRIIEESFDGSAGTVVMQSDLMEADFLAAAHGHKMNGCGVVTSSIHADISYTLGSYLLKKMSPSSGDIAMNVADLEVVKGLVAQKNTKVPQMIQVSISTENINTGTAQLRWQNVNAQGIPDEPFATATLEYGDRSAWLSSWVPQTHLIQGRIEALETLAAQGIANRLSHNMAYLLFANNLVDYATKYRGMQSVVINGLEAFADVTLTTEKGGVWTVPPYFIDSVAHLAGFVMNVSDAIDTKANYCVTPGWSSMRFAKPLVAGAKYRSYVKMIPTVEDRSVYLGDVYVLQDNEIIGIVGGIKFRQYPRILLNRFFSAPDEASPSPNPTHGATAKAQAQPKTIAASHGDEKHFATSVQISTPSKIGHQLIVKPVAIQAPPPAGPVVVALKDPEVQGSPVVVASAAAAVDTNSVASKAISMIANEAALEQADLQDEASFAELGIDSLMSLVISEKLREELGVVVSGSLFLEYPTIGDLRSWLMEYYG
What is the function of this protein?
Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of dibenzodioxocinones such as pestalotiollide B, a novel class of inhibitors against cholesterol ester transfer protein (CEPT) (PubMed:31474098, PubMed:28005187).  The biosynthesis initiates from condensation of acetate and malonate units catalyzed by the non-reducing PKS pks8/GME11356.  Pks8/GME11356 lacks a thioesterase (TE) domain, which is important to the cyclizing of the third ring of atrochrysone carboxylic acid, and the esterase GME11355 might play the role of TE and catalyzes the cyclization reaction of the C ring.  The lactamase-like protein GME11357 (or other beta-lactamases in Pestalotiopsis microspora) probably hydrolyzes the thioester bond between the ACP of pks8/GME11356 and the intermediate to release atrochrysone carboxylic acid, which is spontaneously dehydrates to form endocrocin anthrone.  Endocrocin anthrone is further converted to emodin via the endocrocin intermediate.  Emodin is then oxidized by several enzymes such as the Baeyer-Villiger oxidase GME11358, the oxidoreductase GME11367, the short chain dehydrogenase/reductase GME11373, as well as by other oxidoreductases from the cluster, to modify the A and C rings and open the B ring, and finally yield monodictyphenone.  The prenyltransferase GME11375 may catalyze the addition reaction between the C5 side chains and the carbon bone of dibenzodioxocinones.  The remaining biochemical reactions to the final product dibenzodioxocinones should be methylation catalyzed by methyltransferase GME11366 and reduction and lactonization reaction catalyzed by a series of oxidordeuctases (Probable).
What are the official names of this protein?
Non-reducing polyketide synthase 8, Dibenzodioxocinones biosynthesis cluster protein pks8.