Protein: GRA1_GIBZE
Sequence: MALLNGKSTLPNGHNSSIESPNGYTEHEMPIPSDWQRYLIDIDHAAVSLFKSPRPDDPVATVKSTHRFVLTEETLLVSIPSTIYAAFAIVMSEYSNSQDVLFGVRAESRVVPFRVLVDKNDQISSFLDQVASKWKMAQNFPPDMPVPAVPNVLAFVDDKMSASGNGLVLEENEEISMVISLDSKEITIQVLFNPACADLVAVQRFLKQLETVFHQLCKPSAGQLIKEVKSITADDIRDMTTWNSASMTPYTPECIHDVVKQHVLASPNSCAVHGWDGDLSYVQLDEESSRLANYLYRKGVRPHDLVPLAFYKSIWFTICALALSKLGAAIVPLDPQWPKDRQMYIINDIESSRIITNIPNSASAYAGLEIIDISQLSLANEPATARYPVTPEHAIYAYYTSGTTGQPKGCVIEHGAFVSSSSKRIKFMGRNKDSRLLQVTTYTFDVAMDDIFFTLMAGGCLCVPTREECLNDIVGAVEKYNCNTLHVAPSLARDLQPSQLPSLRTLILGGEAMSANILRTWAGRVGLYNSYGPTECCIACCVNLIQSADENPRNIGRPIDCSYWLVRPGDIDTLAAIGTVGEILIHGPNLGRGYLNKPELTAKAFPQNISWASEVGLPAEARFYRTGDLARFNADGSVCLLGRIDDQVKIHGQRIELGEIDYRLSQCLPAGIEAISGVVNFRDREVATLVAFIQTVNSDSNSAKSSGSLALADTDNWNQFHRLRSHVMEQLSHNLPSYMVPSVFLPVQNFLYFNQGKLNRRGMFQQASRFRLDDILTINTSAGHKADDTSSWSAEALVLRQLWAIALGLEEKNIHLDSRFADLGGDSLAAIRLGILCRPYDIELSVDDILQQSTILLQAEMSEKKKKQNIEKHEVAQEAILPAGQRFGLLGQDVDVGMLCEQTSAQCHIDSGAIDDIYPASPLQESLMALSMDDSPYISQFVFRLPDNLDMYRFRKAWKSTFSDIPILRTRIIYLQDFGTLQVVVDEDIEWTEHKNITLSQFLKDDQERLMQVGDRLVRFTIVQESPGTSFLVWTCHHACHDGRTVDQTLRVVGAKYLDRPVSSPVPYRYYIQFQQDVYRRDWQEYWTRNLAGASVSAFPAQDKAVHQPVTDASHQYSFSMPRISTERSDTSVFSPSSILRAAWALIVSRYEESEDVTFGTIVGANASTIADADAIVGPTNNTIPVRVLASEGWTVEAFVSHVKQQFEYSPFQHVGLANLKDLSPEMQDIVNIRNIFVVQSHFVGQTGSEMNLERVAVSAHEGFKYPLVVECFQENASQVLVNFIYDSHILDQSQISRLALQLENTINLLVHNPHKTLNQIEILSPSDVAQILEWQADMPSPSSLCLHQQFFTQVKRSPDAIALCTWEGQFTYLEVQNLVESMAIYLQDAGVRRGDRILCQIEKSACAVISFLAILKLGGTCVLLGTTWPRIRSEVIAEDTKAQYLLVSPTLSNALISLVPNILEVSTSFIQRLPRPTQYVDSVYQPSDLAFILFTSGSTGTPKGVQLAHSGLVTNFASMAQHMQYTSETRLFQFSDFTFDLSIYDIFGMLMVGGCICMPSEQERHEDLMGSMNRMKVNTVTATPSIAKMIKPSSVPTLRCIKLGGEALDSTTLATLAGSLDTENGYGVTECSVWSTCTDRLSPDADPRNVGRGINCYTWIVDAKNPNRLRPIGAVGELILQGPGVALGYVNKPEESKRVFLDALPWSTDKGRSYRTGDLVKYAPDGSLIYVQRKDAQLKIRGQRFESSEVESHLQQCGLPEGNFCVDLVKTQTGPVVVVFLCMNKEVEMKDASKLGVVPLDQQDSSIVDQMAQAMRMLIPRLPGYMIPQAVIPVTQMPVSNSGKLDRRALRSLADGMSPGQLRQLLRPSEDSIYHKRTELETEAERTMAVLWSQVLAIDDSHVFHTDDDFFQLGGHSIALMRLISAGKGHGMSISYRDAFLHPSLGAMSRQATVSDAEEHALPRLIQPFAMAPSDVDGLIQESSRACQVDPQDIVDLYPCTPFQESTMTLSLSKPGLYTAQFVWSLPDTIDLARLRSAWETLVRSDAILRTRLIYSSKYWQVVTRTGIDFALADMSIDSYLEEDKARPIRLGQPLNRLAIIQDQTSATQYLAWTAHHSTYDGHSWSSIQDRLSDLYTQGGSKLPLVPYNVFVDHIVNNPLPETGLSFWKDMMSGARMPSFPKLPLNNDLQSTNSVVTQSVSLPRQTSSDVTVASAIQGAWAILLGQYENSDDVLFAATLSGRNVPVDGIENIAGPTSCTVPMRVRTDPGQSTRSWLRSVQQSYVDAATYGQIGMNEISLVNKDAEVARHIRSLLIVQAITSKTVSGLERIGCTKIETKAKGFLSYALVVECKPSMENNEMEVMVSYDANLLDGPSVYRLVWQLEFTLQQLLSDNCKTIRDLCLISPSDMETISTWNKELPKTVETTVHALFDRRLSQKHSATAISSWDGEMTYVELDNYSSSLAAHLMASGVKPGQYIPLCFEKTMWMVVSMLAVLKAGGACVSLDPNHPSRHHQVILSRVSADIVITSPANKHRFPGNRVLSVSAALMTKIAHEPYAAPLVSAHQPAFVVFTSGTTGEPKGIILEHRALCTSIEAHGQFMEFGPESRVLQFASYTFDVSIAEMLTTLAFGGCICIPSDHARLNNLSGAIKTLRVNQAYLTASVAALLDPDTLNGSLKVLSVGGEQVGQEVLTRWGDRTKLLNMYGPAETTIWCGGKHSVKPDGDAANIGYGVGARMWLTDVNDVQKLAPIGAVGEIVIEGPLLARGYINGNNDVFVESPDWAKAFNVFDGFDQVTGRVYRSGDLGRYQSDGSIAICGRRDTQLKIRGQRVEVSQIEDQLQRLAPDFKCVVGVLRTDTPTLVAFIGLEGPTKDQGLTDSMDLVVRTRDLSEEVRDLMGSLESRLANILPPYSVPAHYLVLRNIPLMTSGKTDRKKLQVIASEHLEHSVDASKPQMLQQVKKIPTTQMEWNLFGLWAQVLGINNLASLGTDDNFFRCGGDSLKAMQLASLASQRGITLQVSDLFKNPVLADLAQAIVLDTPKEIETSPVQDIPDPYSLLPNDTKEQVQAQAALDCDVSPNLIDDIYPCTPLQDGLFALSQKQPGAYVAQFKFSVANRINIRRLRQAWETVCDQAPILRSRLVSTPSGIVQVVLTEDFWWYERHDIDTSAELEQDKAAIGGLGQPLQRFRLVQDVARGQKTLVWTIHHAAFDGWSIERILESVRLVYQDQPIPNPYVPFNAFIRYSSGIVENHESKEFWQSYLSNITPPTFPALPSPSYQALADTVIESKMSNLKLPDSFTLTSILRAAWAIVVSTYQGSDDVVFLTTVFGRNAPLAGIDKIIGPTITTIPIRVKLNDPSTTVDMLLQAVQESATETMAFEQLGLQSIRNLNADCKAACMAQNLLVIQTSRGEDATVPFGGFEKLPDETKGFSTIPFTLECTATSEGGLSIEASFDSKIVDPAQANRIIKTFEHVTQQMCHKHLKLNQVDLISDSDHDLIRNWNSTMPCAKEECIHHRLDRLAVSNPDAEAVCAWDGIFTFKELNSLSNRYAVYLQSQGIKPGNIVPFCFDKSKWVVVAMLAIMKVGAASVTVDPKHPPGRRDGILSAVSASAVVTTSGYTHLFDHNASHGLKTLVLDGKTMDSIADSLQPADIESTPNDAAFVVYTSGSTGTPKAVVIEHRGICTGAFHLAKLIHLGPQTRCLQFAAFTFDQSFGDIFHTLLLGGCVCIPSESDRLNDLVGSILRLRANTAILTPTVACSIDPSELGSHKMDVLTVGGEPVTAEAIRTWAPHVRLFNTYGPAECSVTTIGRPINMQNVTQPANIGRGLGALVWLTYPDDPERLTPIGTVGEILMEGPQLARGYLNDSRNTNAAYITDPAWSHRFPVPGMSTPRRFYRTGDLGQYQADGTIVCLGRRDSQVKLRGQRMELGEVEHHISTYSQSALEIIADVFTPPNGTATLAACISLKGYETKGDECQVEVDEQVLTIFSAMLSGLDSYLSRMLPAYMIPTLYVPVTHIPLTPSGKKDRKSIRLMLARITVDQVQKMRTILGESEPSRPLTEREKDLQQLWVKVLKLDGETVINANTNFFHSGGDSVRAMALVAAARRKGTHLTVAEIFSHPKLCDMAAMTTSLSQKGQPVQLAPYSLLRSTPSADTMSEACGACGVDHSQIQDMYPTTPLQQALVALSIKDSGAYVSNFVFLLPSHLDVELFQRAWECVYVVVSEKVRWNYGDNLEEFVGRQSQKGFKLGQRMAESAIVRQKDGKTFFILILHHAIYDGWSLRRLLEAAEQIYHGQAIPRFVIFNHFVAHVSRSEDNRAPAFWRSYLEGLPKTSFIQQPTTAYKPTADHIISQDVSLRDNFTARSGVTITSLMRAAWAMVLATYNSDQTPDVVFGTVVGGRSLDLADIEYIDGPTIATVPFRVTFDPTAPVDMLLQSVQTMSTQILQHEQFGVQNIIKVSDDGRLACDFETLLVVQDSAEIKASSGFLDMDNIYQRPDRPPGIPLVVECSPSAGNLHLEIHFDAKLLEETQAKRLIRQLAHVIKQLANSVPSLSLSCIDMMNPDDAEEIKSWNKKPPPTFDGCLHEMVLQHSKGCPDRIAIQSWDTSLTYSELDHLSSILAQYLNSLGVRPEDKVPFCMDKSAFAIVAMLAILRSGGCFVPLDMSSPTKRLKNIIKRVNAKFILVSPKTRPLFEDVEGQLVEVTKSMIDGLPELSKSLYIPSATHPAYVLFTSGSTGTPKGVVVEHGAIATSVSSFSSYLGFNPDVRVLQFAPYVFDVSIGEIFACLVSGGCLCIASESSLMDDLPLCIQQLDVNFAVLTPTFARTLTPSEVPSLKTLVLGGEPLRKRDVETWATTVRLFNGYGPTEASVLAMAYRVPDSQSPCNLIGLSVGCRSWIVSPSDPNILPPIGAVGELVLEGNTLARGYLDTESAQGAFIEDPKFLDSLVPEATGSRVYKTGDLVRYNAEGIVEFVSRKDTQVKFHGRRIELEDIEHNAMEAMPEAKHLVVELVRLGNSQQEALALFFHTDNQRTANEKDPILPIGQDLVARLRGVKSNLAVTLPSYMVPSLYIPLSTWPSTSNGKVNRHLLRNLVLHFTSEAAAAYSLHTGDSSALSSDEESQLAQLWATTLNIDARTIGSSDSFFQHGGDSIAAVRLVTLAREQGIGLSVDTLLSKPILRDMALCMTSAQPVRETIVRPFNQIHSHQEEVLLAASQFGVEPAMIEDIYPCSALQNSLMAVSLKNSSAYLSQFVVAIPEGLDIDVLQAAWNTVYSDSPILRTRFYQPSLNNMQHPILQAVVDQKPIWGTEENLDEFLGRDKQTPTGLGSPLTRFTIVVDKTNQERLFVLTAHHAIYDGWSIASTFEKVDMILKGIALPKSVGYNIFIHHLQSLNTQENKAFWSSTLEGATQTLFPQLPSHSYEPATDNSLKHQFAYPADLAPHSTVTMATIVRGAWGLLVSKYCDSPDVVFGTTVNGRMAPIPGIEMVQGPTMATIPFRTRISTNQSVLSYLEQLHVQQIESIPHEQYGLQNIKHLSEPIARVCEFQNLLVIQSSRDSSLSDSGFAFGTVKNMDQGSFSMQGFHNMALVVECSIDSEVIHVTLNFDSNVIPKTQVQRIAHHFEHLILQLQAGSTEPDLKIDQIDHVSPSDQAEILEWNSSIPGSVLCCVHELFESRARLQPSAPAICARDGQLTYFELQAKATTLAAYLSLQGLGRGVLVPLLFEKSCWAVVAMMAVLKAGAANVALNPEHPQARLEDSINATQGEVILCSRKHFELASSFDMQVIVVDEDLFHHIDLPSLASSDPWSPTYPAGPDDPCFVLFTSGTTGKPKGIVINHAAMCSSINGHSSTLRYSTGPGSRNFQFTAYTSDVSIGEIFTSLAVGSCVCVPSDYDRMNNLAGSMRDLNVTWAFLTPSVAALLKPEEVPCLRTLLFGGETATPENISTWADSLYLINSAGPAECCIWTHCNPGISTADIGSNWGYNLGCATWITDPNNPSVLMPIGVTGEMLIEGPNLAQGYLNDPERTQKSFVEIYLAGKKRRLYRTGDLARFMADGKTQFLGRRDTQVKLRGQRVEIGEIENQIRRHIPDSTLVAVEMVRIAEGKSAPLLAAFHAPKDPRAIDDTGDTPQAEVLSAAMVQELGVILDELAAKLAETLPQHMIPTAFIPLTSMPLTASAKTDRNVLSALASTISVEQLSYYALTSAEKQFPSSLAEQQMAKLWQEVLNTKIDIGIHDSFFRIGGDSISAMHLVSRSRAVGISLTVEQIFKNPTLQHMAAIATTFTESMGSTTVEPFSLISPTVTFDIVCSEAQEQCQVTAQQIQDIYPCSALQEGLLALSLKTAGSYLAQMVFEIPDELDLERFKDAWAHMVAKAAPILRTQFFESPSQGHQLMQAVIDAPLEWTYSDKLDEYLMTDSTKIVQLGQPTSRYAIISNTQRYFVFTAHHAVYDGASLGPMFEAVEKIYSENYVSSSSPYSLFVQYLLGMDSDSSKAYWEMSLQGASAPTFPRLPSIGYRPMTNDALKRTIALPARHDTEFTMSSIARAAWSLVVASYSDTDDIVFASTVGGRTLPIAGIENIIGPTLATVPVRVTIDRTASVSDFLTMIQEQSTSMMPHEQYGLQNIKRISPSVSAASDLHNLLVINTSSVEGLGSGGLGLKQVDLGRADGFHNFALSIECTAEADALSLAVSFDDHVIDPRQIRRVVNQFEHMLQQLSTCAIHTRLADMDLTGPADIAEIHLWNSHVPPPQQNYVHTLVEQRVKSQPDSPAVCSWEGELTYRELDELSSSLANHLITAFAVAPGTLIPILFEKSIWTVVTMLAVLKAGGANVPMDPQQPLARLQELAADIGASLAISSSKYQDKAQNVTARSMFVDREVLTTIEKTPICPASTVSYEDPAFILFTSGSTGKPKAILIDHTAFTSSIKGHGEILRYRKGSRNLQFTAYISDVSIGEIFTSLSAGACVCVPSDFERMNDLAGSINRMRVDWAFLTPSVASLLDATKVPSLKTLVFGGETATPENIAAWAPRLFLINSFGPAECSIWTHCDPGVGITHNGSHIGYAIGCATWIVDPNDYNKLAPIGSIGELIVEGPNVARGYLDEAKTKEAFLKTAAWMPSGRKNRLYKMGDLVRYLPDGKIQFLGRKDSQIKLHGQRIELGEIEHQLRVALAKHDADRNVQVAVEMVSLSTDTSTSSLLTAFVDYEGISSDDGPAQLSSGEKAQQWARQMFRVAHEHLALTLPRHMIPSVLLPLTRMPLNGSAKTDRKVLKQIVSGMDTMQRALYSLARVETNIIKAATPNEKTLHHIWSEILSISPESFGVEANFMSLGGDSIAAMKLIPIAQAAGLSISVEDLFTRPVLHDLARVSRQSITEHSQDIPPFYIMEQAQNQDELLAEASTHCNLPPDAIHDIYPCTQVQERFISTTQIQPGAYTLQDVFKISSDMDLAQFKKAWTRTVASHVALRTRIFLSNDRHQHLQVVQKASETLDWIHSENLEEYLKADKAKSMEYGGSLVRSAIVSEGVERHFVVTYHHSIYDAVSLGIIMNDLEAFYLDDLYEVNEPKYNAFVHHLTQVKSQELSSQFWRDHLAGDRSTITPLYQPVDGARVDSLLRHTITFPMHYQQSQLSLTVAAFTYAALSLVTARLTGSSSAVLELTLLGRSVPVKGIERMVGTTVTSAPLRIDTTAGNDKPWTVTVEDYLDYAKKRASSIVLHEHTSMPDPETKHITSAALPIVVHPSNPHKEALGTGIGLQRHEIQSMGQNSSAFYMDIAALDGEGLEINLPFDMPLDEVTRNVDHETDLRMCDIIEQEFRGYIILALVNRLNFVPSCWTMGRWLNEAPRSTY
What is the function of this protein?
Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of gramillins A and B, bicyclic lipopeptides that induce cell death in maize leaves but not in wheat leaves (PubMed:30395461).  The nonribosomal peptide synthetase GRA1 incorporates respectively a glutamic adic (Glu), a leucine (Leu), a serine (Ser), a hydroxyglutamine (HOGln), a 2-amino decanoic acid, and 2 cysteins (CysB and CysA) (Probable).  The biosynthesis of 2-amino decanoic acid incorporated in gramillins could be initiated by a fatty acid synthase composed of the alpha and beta subunits FGSG_00036 and FGSG_11656 (Probable).  The cytochrome P450 monooxygenase FGSG_15680 could hydroxylate the fatty acid chain (Probable).  Subsequent oxidation to the ketone by the oxidoreductase FGSG_00048 and transamination by aminotransferase FGSG_00049 could form 2-amino-decanoic acid (Probable).  On the other hand, FGSG_15680 could also be responsible for the HO-modified glutamine at the gamma-position (Probable).  Whether hydroxylation occurs on the fully assembled product or on the Gln residue prior to assembly into the gramillins requires further proof (Probable).  The thioredoxin FGSG_00043 could also be required for the disulfide-bond formation between CysA and CysB (Probable).  The specific involvement of the remaining proteins from the cluster is more difficult to discern, but could have broader regulatory (FGSG_00040 and FGSG_11657) or enzymatic functions (FGSG_00044 and FGSG_00045) (Probable).  The final C-domain of GRA1 does not possess the expected sequence of a termination CT domain, often implicated in macrocyclization and release of a cyclopeptidein fungal NRPs; and the thioesterase FGSG_00047 may act in concert with the terminal C-domain of GRA1 to catalyze the formation of the macrocyclic anhydride and release of the products (Probable).
What are the official names of this protein?
Nonribosomal peptide synthetase GRA1, Gramillins biosynthetic cluster protein 1, Nonribosomal peptide synthetase 8, NRPS8.
What is the protein family that this protein belongs to?
Belongs to the NRP synthetase family.