Protein: CDIA_ECOL5
Sequence: MHQPPVRFTYRLLSYLISTIIAGQPLLPAVGAVITPQNGAGMDKAANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLKAGGEAKGIINEVTGGNRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTTGRPVMNADGSLQALEVTEGSITINGAGLDGTRSDAVSIIARATEVNAALHAKDLTVTAGANRITADGRVSALKGEGDVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARDGDIILSSAGKLVLKNSLAGGNTTVTGTDVSLSGDNKAGGNLSVTGTTGLTLNQPRLVTDKNLVLSSSGQIVQNGGELTAGQNAMLSAQHLNQTSGTVNAAENVTLTTTNDTTLKGRSIAGKTLTVSSGSLNNGGTLVAGRDATVKTGTFSNTGTVQGNGLKVTATDLTSTGSIKSGSTLDISARNATLSGDAGAKDSARVTVSGTLENRGRLVSDDVLTLSATQINNSGTLSGAKELVASADTLTTTEKSVTNSDGNLMLDSASSTLAGETSAGGTVSVKGNSLKTTTTAQTQGNSVSVDVQNAQLDGTQAARDILTLNASEKLTHSGKSSAPSLSLSAPELTSSGVLVGSALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSGLITGDNGLMLNAVSLSNPGKIIADTLSVRATTLDGDGLLQGAGALALAGDTLSQGSHGRWLTADDLSLRGKTLNTAGTTQGQNITVQADRWANSGSVLATGNLTASATGQLTSTGDIMSQGDTTLKAATTDNRGSLLSAGTLSLDGNSLDNSGTVQGDHVTIRQNSVTNSGTLTGIAALTLAARMVSPQPALMNNGGSLLTSGDLTITAGSLVNSGAIQAADSLTARLTGELVSTAGSKVTSNGEMALSALNLSNSGQWIAKNLTLKANSLTSAGDITGVDTLTLTVNQTLNNQANGKLLSAGVLTLKADSVTNDGQLQGNATTITAGQLTNGGHLQGETLTLAASGGVNNRFGGVLMSRNALNVSTATLSNQGTIQGGGGVSLNVTDRLQNDSKILSGSNLTLTAQVLANTGSGLVQAATLLLDVVNTVNGGRVLATGSADVKGTTLNNTGTLQGADLLVNYHTFSNSGTLLGTSGLGVKGSSLLQHGTGRLYSAGNLLLDAQDFSGQGQVVATGDVTLKLIAALTNHGTLAAGKTLSVTSQNAITNGGVMQGDAMVLGAGEAFTNNGMLTAGKGNSVFSAQRLFLNAPGSLQAGGDVSLNSRSDITISGFTGTAGSLTMNVAGTLLNSALIYAGNNLKLFTDRLHNQHGDILAGNSLWVQKDASGGANTEIINTSGNIETHQGDIVVRTGHLLNQREGFSATTTTRTNPSSIQGMGNALVDIPLSLLPDGSYGYFTREVENQHGTPCNGHGACNITMDTLYYYAPFADSATQRFLSSQNITTVTGADNPAGRIASGRNLSAEAERLENRASFILANGDIALSGRELSNQSWQTGTENEYLVYRYDPKTFYGSYATGSLDKLPLLSPEFENNTIRFSLDGREKDYTPGKTYYSVIQAGGDVKTRFTSSINNGTTTAHAGSVSPVVSAPVLNTLSQQTGGDSLTQTALQQYEPVVVGSPQWHDELAGALKNIAGGSPLTGQTGISDDWPLPSGNNGYLVPSTDPDSPYLITVNPKLDGLGQVDSHLFAGLYELLGAKPGQAPRETAPSYTDEKQFLGSSYFLDRLGLKPEKDYRFLGDAVFDTRYVSNAVLSRTGSRYLNGLGSDTEQMRYLMDNAARQQKGLGLEFGVALTAEQIAQLDGSILWWESATINGQTVMVPKLYLSPEDITLHNGSVISGNNVQLAGGNITNSGSSINAQNGLSLDSTGYIDNLNAGLISAGGSLDLSAIGDISNISSVISGKTVQLESVSGNISNITRRQQWNAGSDSRYGGVHLSGTDTGPVATIKGTDSLSLDAGKNIDITGATVSSGGTLGMSAGNDINIAANLISGSKSQSGFWHTDDNSASSTTSQGSSISAGGNLAMAAGHNLDVTASSVSAGHSALLSAGNDLSLNAVRESKNSRNGRSESHESHAAVSTVTAGDNLLLVAGRDVASQAAGVAAENNVVIRGGRDVNLVAESAGAGDSYTSKKKKEINETVRQQGTEIASGGDTTVNAGRDITAVASSVTATGNISVNAGRDVALTTATESDYHYLETKKKSGGFLSKKTTHTISEDSASREAGSLLSGNRVTVNAGDNLTVEGSDVVADQDVSLAAGNHVDVLAATSTDTSWRFKETKKSGLMGTGGIGFTIGSSKTTHDRREAGTTQSQSASTIGSTAGNVSITAGKQAHISGSDVIANRDISITGDSVVVDPGHDRRTVDEKFEQKKSGLTVALSGTVGSAINNAVTSAQETKESSDSRLKALQATKTALSGVQAGQAATMASATGDPNATGVSLSLTTQKSKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGSQLKVGGNTSLDAANDILLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFAGVNAAKGSEKGNGTEWTETTTDSGKTVTINSGRDTVLNGAQVNGNRIIADVGHDLLISSQQDTSKYDSKQTSVAAGGSFTFGSMTGSGYIAASRDKMKSRFDSVAEQTGMFAGDGGFDITVGRHTQLDGAVIASTATPDKNHLDTGTLGFSDLHNEADYKVSHSGISLSGGGSFGDKFQGNMPGGMISAGGHSGHAEGTTQAAVAEGTITIRDRDNQKQNLANLSRDPAHTNDSISPIFDKEKEQRRLQTVGLISDIGSQVADIARTQGELNALKAAQDKYGPVPADATEEQRQAYLAKLRDTPEYKKEQEKYGTGSDMQRGIQAATAALQGLVGGNMAGALAGASAPELANIIGHHAGIDDNTAAKAIAHAILGGVTAALQGNSAAAGAIGAGTGEVIASAIAKSLYPGVDPSKLTEDQKQTVSTLATLSAGMAGGIASGDVAGAAAGAGAGKNVVENNALSLVARGCAVAAPCRTKVAEQLLEIGAKAGMAGLAGAAVKDMADRMTSDELEHLITLQMMGNDEITTKYLSSLHDKYGSGAASNPNIGKDLTDAEKVELGGSGSGTGTPPPSENDPKQQNEKTVDKLNQKQESAIKKIDNTIKNALKDHDIIGTLKDMDGKPVPKENGGYWDHMQEMQNTLRGLRNHADTLKNVNNPEAQAAYGRATDAINKIESALKGYGI
What is the function of this protein?
The CdiA protein is thought to be exported from the cell through the central lumen of CdiB, the other half of its two-partner system (TPS).  The TPS domain probably remains associated with CdiB while the FHA-1 domain forms an extended filament with the receptor-binding domain (RBD) at its extremity; in the secretion arrested state the C-terminus of the RBD and YP domains form a hairpin-like structure as the FHA-2, PT and CT domains are periplasmic.  The YP domain is probably responsible for this arrest at the point where it re-enters the host cell periplasm.  Upon binding to a target cell outer membrane receptor (heterotrimeric OmpC-OmpF for this CDI) a signal is transmitted to activate secretion.  The filament elongates slightly, the rest of CdiA is secreted and the FHA-2 domain becomes stably associated with the target cell's outer membrane where it facilitates entry of the toxic CT domain into the target cell periplasm.  From there the toxic CT domain is cleaved and gains access to the target cell cytoplasm via an inner membrane protein (FtsH for this CDI).
What are the official names of this protein?
tRNA nuclease CdiA, tRNase CdiA, Anticodon nuclease CdiA, CdiA-EC536, Toxin CdiA.
What is the protein family that this protein belongs to?
In the N-terminal section; belongs to the CdiA toxin family.
What are the subcellular locations of this protein?
Secreted. Target cell membrane. Target cell, target cell cytoplasm. Note=Secreted to the cell surface by CdiB, its two partner secretion pathway (TPS) partner (Probable). Expressed on the cell surface of both the encoding cell, and in mixing experiments on the target cell surface, in a proteinase K-sensitive manner. Both the N- and C-termini can be detected on target cells, whereas only the CT domain is detected in the target cell cytoplasm (PubMed:23469034). Toxin translocation into the target cell depends on the proton motive force of the target cell, but not on tolA or tonB (PubMed:25174572).