Protein: LUC5_FUSSX
Sequence: MGAPNSTREPIAIVGTACRFPGGANTPSKLWDLLCEKRDVQTRIPPERFNPDAFYHRNGEKSGCTDVKKAYLLTEDIRAFDASFFKINPREAEAMDPQQRLLLETVYEATEAAGLPYEDLKGSNTAVYVGSMTGDYHEMLLRDPQDMPKYMATGTARSILSNRVSYFFDWKGPSMTIDTACSSSLVAVHEAVTALRLGVSNIACAAGTNLILGPEMMISESKLHMLSPTGRSKMWDASANGYARGEGTAAIMMKTLSQALSDGDHVYGIIRETGVNSDGHTNGITLPSSESQKTLIRQTYANAGLDLIKERCQFFEAHGTGTPAGDPIEARAIHEAFFEDAAGSSDQMFVGSVKTAIGHLEGCAGLAGLIKALEAVRRGVIPPNQLFENLNPALKPFAGNLSIPTETLPWPEVAPGTPRRASVNSFGFGGTNAHAIIESFDNTPQPAPTGGIISYPLVLSANSEKSLRRQISQLHDTLQNAGEGEVQDTLYTLAQRRSQLPARTYFSGHTQEELLKKLSAASAEDATITVASQEVTNQNSRILGVFTGQGAQWPTMGREILKSSAFAGDLITRLETSLASLQEPPTWTLSEQILADPESSRLGEAAVSQPVCTAVQLMLVELLRQAGITFSTVIGHSSGEIAAAYAAGFLTPEDAIRIAYCRGVCAKLAGGEEGQKGSMMAVGLSYEEAACFCEDHFPGCIDVAASNAPSSATLSGDKDAILEAKALLDEQGTFARVLKVDTAYHSRHMQPCAEPYMALLRESNIQLLPGDDSCEWFSSVIGERMSSFTHGQLLTGEYWVENMVKPVLFTLASELAADSKLPCHVALEVGPHPALKGPFSQTYKRATGSQLPYQGALTRNVHDVEALSDALGFIWARLGKSAVNFASHAELFSVSKTSFSTNLPSYPWDHSQSFWKESRKSANFRQRTSPPHPLLGTRSTEDATQDLRWLNILHLDDAPWLEGHKVEGLVVYPAAAYLVMAMESAKSIDETKTIQLVELFDVQILSAIQLSQDSQGVETLFTLEIDDVNSTAATARWSLFTSMVGRGSNWKCNAKGHLRVDFGSEAQDSLLPSRDPPVASLTSVNIERFYTSLAEIGLGYTGAFKHLATVQRQSGFATAKASQMNTDFSAMIHPALLDSAFQSLFAAYCWPDDGSLAAPFVPTFFKSLRIVSLDHIENGQELTIDSYLTDTNDREITADLDIFTSDSEKPLLQLQGLTCTSLLRPGPSNAKELYTQTKWEVDISCAVASLDVQQHDAAEDLDLVDLCERLSYYYLRELNRKVDRSEVPAMDWHFQRIFEWIDYLFPIIEAGKHTTIRKEWSADEGSWLLEQASKFPGQVDLLLIRAVGENLTEVVRKETTMLEHMVRNDVLNRFYKFGLGFQRANGYLSRISKQIAHRYPQMKILEIGAGTGGATKGILESLGTTFESYTFTDISTGFFEAAAEAFEPWVSKIIFKPLNVENDPVEQGFLEAQYDFIVASNVLHATKSLSTTMRNVRRLLKPGGQLLLLEVTSDIVRVRLMMSGLSGWWLGGDDGRRYAPTITVPEWDSLLRSTGFSGVDHTVNDFYDPSKYMTSVMLSQAVDDHHVDILRKPLNSALGWLPQRCITIIGGKNNEIAQQVSKTLLSMKSASLDLINHVDSFEQLASTPELPLRAVLILEDLDEPVLKSLTSEKLAGLQRTINDSRQILWVSKGCQKDEPYANMSTGLCRSLASEYPHIQLQHIDMETGLDSLAVSRIVEALIRIVYKASLKQDDDLLWSHEAELILEDEGRWLIPRILPDDKLNDHLNAGKMKVKTNASLADTPVEIQQAGSQWVISQTVPSLPISDNTDHIRIKASYSTLHAVRVRGSRVYLSYGHRVTGSTTPVIAFSETAGSIISVPESQVFDVPQGFDIDQSASLRSLVLTAIVESVLAECDHGAAIIVHEADNYLGAAFETKCREIGLKLVRTTSKSDHKDDAIFIHPLAPERVVKKALPHVEVAVVVDLSGRDYSVVDSPLRRHVPSTTKFLELSDLIGSVTCGLRDVNIQCVQDAIESSFKSPSDGPVVNISEVSGLQASETSYATVVDWSIEKPVSVQVQPLQANRLLRSDRTYLLAGCTGGLGKALCRWMVAAGVRHLALTTRNVEAIDKVWLEGLQLQGADVRLFQVDVGDKAALERAHAQVTAEMPPICGVANAAMVLSDRSFGELKVGDFDKVFGPKVRGTQNLHELFQDEPLDFFIMFSSLASVVGNRGQANYAAANLFMTAVAEQRRAKNLAASVIHIGMILGVGYVSSTGAYEATLRQYNYMPISEPDFLNMFSEAILVGQPGSSHAPELITGLNRYSLQEDAPKFFWHENMRFSHHTLEEQHQESTSTTKASISQRLAQVQTPAEMLEVVEEEFCTKLERMLQAESGTIKVSQPLMSLGVDSLIAAEIRSWFFKELDVDMPVLEILNTASVAEICSTAVASLATLAPQEQTETTTLVTSEAVQSLNAVSGNGSSSSRAPTEFNSSTLKSGAQSTQGTSVSGDKDTNSVDGSAKVERNGPLSFAQERIWFLQQYLQDATTFNVTMAYRITGPLRVNDLESAFQKVIQRHESLRTGFHMDPETTVPTQIVYEQSPFGLEQRNDSDITKEFEELQNTHYDLENGRVLKAIVLTKPDTDEHILLVGFHHIALDGFSAQILVRDLAIAYAGGNLAPLDKGYLDFAVDQRAAVYPAETLQYWKTEFETLPPALPVFDFAETKTRLPLTDYKTRVSERTLQPDVAGKAKSAARALAATPFHVYLAALQVLLSDFASTQDVCIGITDANKNDAAHMDTIGFFVNLLPLRFQLSASQTLAELVSNTKAKANGALTHSRLPFDVLLDELKIPRSTSHSPLFQVVLNFKMGSTQKVPLADCQAEVIDFKDVNNPYDLAFDIETYPDGSTSISVKSQEYLYTKNELDLILESYINLLSLFEKDSSKTLGEVSQCTPDEAQKTLTLGRGERIPSPSFDTLSHYFEDWVKRQPDAIAIRDDQGTTLSYSQLKSFVNNIAATLEKSGLTPGARVGVYCEPSIFIIASLLAIAEVGGVYVPLDPQNPIKRLQLIVDDCEPEILLFDESTKELAPKLQTNASLINIYNVRRLPSSAAITNRAQGAGMAYMFYTSGTTGVPKGVALTHANLVHHIDSITHFYDIKRGTMLQQAPLGFDMSLTQMSLSTMLGGTLVVASSEARKDPLQLAKLMLSERVTHTFMTPTLAVALIHHGYEYLVKCVGWEFSLLSGEAFRTHVISEFQRLGLPQLKLFNGYGPTEITINSSSGLNELDLAAPRDTRNPTIGFTLPNYSCYILDEDLKPVRPGHAGELFVGGAGIAVGYLRRDELNKERFLSDPFASSEDVARGWARMYRTGDKAKFLPDGRIVFLGRIAGDSQIKLRGFRIELEDIANTIVKSSGGVVSEAAVSFRQGVNGPDDGAFLVAFAIISQAHRPENPSSFLKQLLKDLSLPRYMIPAKIVQVEHLPMGPTGKLDQNALDVMPIPQDENVHEETLTTTQERLRALWFESLPAVAPDAFIGSETDFFEAGGNSLRIVMLREHIAREFGVMVSVFDLFQASTLGGMAAKIDGSTGADNQPIIWEEETRVDIPSGLETPDEPAILDGDELEVALTGATGFLGLAILRSLLKDERISRVHCLAVRSPSKARDEVFKSPRVVVYHGDLSSPRLGLSEDEFGTLSKKFDIIIHNGAEVSFLKSYQALKKANVSSTKELAQLASGRQIPFHFVSTGGVVNLTDHDGLPEISVSGFKPPIDGTEGYAASKWASEVILESHAERAHLPVWIHRPANVTGAAAPATDLMGSILQYSTTMQSLPEISNWKGSFDFVPVEQVADEIAASIHESRSSEPVYRHHCGDQKISVSELSAHLEAGIGAKMEIIGVDDWLARARSTGIDETTALLVEKMLSGENGGTVPWLRKGE
What is the function of this protein?
Hybrid PKS-NRPS synthetase; part of the gene cluster that mediates the biosynthesis of the mycotoxin lucilactaene and the lucilactaene-related compound NG-391 that act as cell cycle inhibitors with potent growth inhibitory activity against malarial parasites, moderate growth inhibitory activity against cancer cells, and no activity against bacteria and fungi (PubMed:32043422, PubMed:35484225).  The hybrid PKS-NRPS synthetase LUC5 is responsible for the condensation of one acetyl-coenzyme A (CoA) unit with six malonyl-CoA units and the amide linkage of the arising heptaketide and homoserine, subsequently releasing the first intermediate prelucilactaene B, as an alcohol with an open ring structure (PubMed:32043422).  Lucilactaene and NG-391 lack the 7-methyl group present in fusarins which is inserted in fusarins by the C-methyltransferase (CMeT) domain of the fusarin synthetase FUS1, suggesting that the CMet domain of LUC5 does not methylate this position (PubMed:32043422).  Within the pathway, both the cytochrome P450 monooxygenase LUC2 and the hydrolase LUC6 function in parallel in modification of prelucilactaene B.  LUC6 may catalyze the 2-pyrrolidone ring formation to form prelucilactaene C from prelucilactaene B, followed by C-15 hydroxylation by the same enzyme to give prelucilactaene D, which is then converted to prelucilactaene E by epoxidation, and finally to prelucilactaene F by cyclization.  Prelucilactane D, prelucilactaene E, and prelucilactaene F can be converted to dihydrolucilactaene, NG391, and lucilactaene, respectively, via C-20 methyl group hydroxylation by the cytochrome P450 monooxygenase LUC2.  However, LUC2, unlike FUS8 in fusarin C biosynthesis, is not enough for the full oxidation of the C-20 methyl group into carboxylic acid, which is a prerequisite for the final methylation step.  The aldehyde dehydrogenase LUC3 is involved in the biosynthesis by further oxidation of the C-20 alcoholic analog prelucilactaene G into a carboxylic derivative.  This unidentified carboxylic derivative may be converted to demethyllucilactaene.  As the last step, the methyltransferase LUC1 methylates the hydroxyl group at C-21 of demethyllucilactaene to generate lucilactaene (Probable).
What are the official names of this protein?
Hybrid PKS-NRPS synthetase LUC5, Lucilactaene biosynthesis cluster protein 5.
What is the protein family that this protein belongs to?
In the C-terminal section; belongs to the NRP synthetase family.