Protein: TCDA_CLONO
Sequence: MLITREQLMKIASIPLKRKEPEYNLILDALENFNRDIEGTSVKEIYSKLSKLNELVDNYQTKYPSSGRNLALENFRDSLYSELRELIKNSRTSTIASKNLSFIWIGGPISDQSLEYYNMWKMFNKDYNIRLFYDKNSLLVNTLKTAIIQESSKVIIEQNQSNILDGTYGHNKFYSDRMKLIYRYKRELKMLYENMKQNNSVDDIIINFLSNYFKYDIGKLNNQKENNNNKMIAIGATDINTENILTNKLKSYYYQELIQTNNLAAASDILRIAILKKYGGVYCDLDFLPGVNLSLFNDISKPNGMDSNYWEAAIFEAIANEKKLMNNYPYKYMEQVPSEIKERILSFVRNHDINDLILPLGDIKISQLEILLSRLKAATGKKTFSNAFIISNNDSLTLNNLISQLENRYEILNSIIQEKFKICETYDSYINSVSELVLETTPKNLSMDGSSFYQQIIGYLSSGFKPEVNSTVFFSGPNIYSSATCDTYHFIKNTFDMLSSQNQEIFEASNNLYFSKTHDEFKSSWLLRSNIAEKEFQKLIKTYIGRTLNYEDGLNFNKWKRVTTSELLKVIEEVNSTKIYENYDLNMILQIQGDDISYESAVNVFGKNPNKSILIQGVDDFANVFYFENGIVQSDNINNILSRFNDIKKIKLTLIGHGENVFNPKLFGGKTVNDLYTNIIKPKLQHLLEREGVILKNKYLKINILGCYMFTPKVDINSTFVGKLFNKISRDLQPKGFSKNQLEISANKYAIRINREGKREVLDYFGKWVSNTDLIAEQISNKYVVYWNEVENTLSARVEQLNKVAEFAKDINSIIQTTNNQELKQSLVNTYADLITTLYSELLKEDIPFELDNIQIKERIILNEISRLHDFSNIILDFYQKNNISNNMIILFDSIIKEKDYYNVKLANKITGETSVIKTYSDSLWNFTNKYKKIVDDIKGIIVKDINGEFIKKADFEIEQNPSLLNSAMLMQLLIDYKPYTEILTNMNTSLKVQAYAQIFQLSIGAIQEATEIVTIISDALNANFNILSKLKVGSSVASVIIDGINLIAALTELKNVKTNFERKLIEAKVGMYSIGFILESSSLISGLLGATAVSEILGVISVPVAGILVGLPSLVNNILVLGEKYNQILDYFSKFYPIVGKNPFSIQDNIIIPYDDIAITELNFKYNKFKYGYAKISGLKVGLVTHIGENIDHYFSAPSLDHYIELSIYPALKLNDTNLPKGNVVLLPSGLNKVYKPEISAIAGANSQEGNGVEVLNLIRNYYVDSNGNTKFPWKYEAPFEYSFSYMRVEYFDTKVNVILDNENKTLIIPVLTIDEMRNKISYEILGDGGQYNVILPVNQTNINIVSNKNDIWNFDVSYIVKESKIEDNKFVLDGFINNIFSTLKVSNDGFKIGKQFISIKNTPRAINLSFKINNNIVIVSIYLNHEKSNSITIISSDLNDIKNNFDNLLDNINYIGLGSISDNTINCIVRNDEVYMEGKIFLNEKKLVFIQNELELHLYDSVNKDSQYLINNPINNVVKYKDGYIVEGTFLINSTENKYSLYIENNKIMLKGLYLESSVFKTIQDKIYSKEKVNDYILSLIKKFFTVNIQLCPFMIVSGVDENNRYLEYMLSTNNKWIINGGYWENDFNNYKIVDFEKCNVIVSGSNKLNSEGDLADTIDVLDKDLENLYIDSVIIIPKVYTKKIIIHPIPNNPQINIINTQSIHDKCHLIIDSVLTNNYHWESDGDDLIITNGLDINIRILQGLSFGFKYKNIYLKFSNYDELSLNDFLLQNYNVKGLYYINGELHYKNIPGDTFEYGWINIDSRWYFFDSINLIAKKGYQEIEGERYYFNPNTGVQESGVFLTPNGLEYFTNKHASSKRWGRAINYTGWLTLDGNKYYFQSNSKAVTGLQKISDKYYYFNDNGQMQIKWQIINNNKYYFDGNTGEAIIGWFNNNKERYYFDSEGRLLTGYQVIGDKSYYFSDNINGNWEEGSGVLKSGIFKTPSGFKLFSSEGDKSAINYKGWLDLNGNKYYFNSDSIAVTGSYNIKGIQYYFNPKTAVLTNGWYTLDNNNYYVSNGHNVLGYQDIDGKGYYFDPSTGIQKAGVFPTPNGLRYFTMKPIDGQRWGQCIDYTGWLHLNGNKYYFGYYNSAVTGWRVLGGKRYFFNIKTGAATTGLLTLSGKRYYFNEKGEQLTLV
What is the function of this protein?
Active form of the toxin, which is released into the host cytosol following autoprocessing and inactivates small GTPases (PubMed:8810274, PubMed:16157585).  Acts by mediating monoglycosylation of small GTPases of the Rho family (Rac1, RhoA, RhoG and Cdc42) in host cells at the conserved threonine residue located in the switch I region ('Thr-37/35'), using UDP-N-acetyl-alpha-D-glucosamine as the sugar donor (PubMed:8810274, PubMed:16157585).  Monoglycosylation of host small GTPases completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption and cell death (PubMed:8810274).
What are the official names of this protein?
Toxin A.
What is the protein family that this protein belongs to?
Belongs to the clostridial glucosylating toxin (LCGT) family.
What are the subcellular locations of this protein?
[Toxin A]: Secreted. Host endosome membrane. Note=Secreted from C.novyi cell into the extracellular environment via help of holin-like protein TcdE/UtxA (By similarity). Binds to the cell surface receptors via the receptor- binding region and enters the cells via clathrin-mediated endocytosis (PubMed:20498856). Acidification in the endosome triggers conformational changes that promote the membrane insertion of the translocation region, allowing formation of a pore, leading to translocation of the GT44 and peptidase C80 domains across the endosomal membrane (By similarity). 1D-myo-inositol hexakisphosphate- binding (InsP6) activates the peptidase C80 domain and autoprocessing, generating the N-acetylglucosaminyltransferase TcdA form, which is released in the host cytosol (By similarity). SUBCELLULAR LOCATION: [N-acetylglucosaminyltransferase TcdA]: Host cytoplasm, host cytosol. Host cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Binding to phospholipids, such as phosphatidylserine and phosphatidic acid promotes localization to the inner face of the cell membrane close to its membrane anchored substrates (small GTPases).