Protein: NOTE_ASPSM
Sequence: MDSIKLTESHQGLSVLHAKQATETMRETLSSSSSPLSLSSITSPLSSASEPPALGEIQARVSESTLFSNAQVPEFWETCVHEVIQQRCREAPESSAVAAWDGSFTYSELDELSNRLASSLILLGVKAETFVPICMEKSRWATVAILGVMKAGGAFTLLDPSYPLPRLKTICEELSSLVVLSSTTQSERCTQLANVIVVEHLCRAWHPGAYLAQSPATVCPSNVLYVAFTSGSTGKPKGVLIEHRAYSTGAREHLRVFQIDQTSRVLQFSSYAFDVNIMETLSTLMAGGCLCVVGEAQRSDVSLFAEAVDELQVSHAMLTPSFARTVPWENVRHLRVLILGGEEMRESDAAICAERGVRLINAYGAAECSVNATARPGVQPGDNLSTIGHGTGAIAWIIDPDDPERQMGPGTAGELLLEGPIVGRGYLNSPDMTDRVFIDPPTWLRQLRMMDYQHQLYRTGDLAVQDSTGALTLLGRKEGQVKIRGQRVEVAEIEQHIDQVLTAATEVVVEKVTPECDQRDVLMAFVQTGRAAQGWTEGSPFFLPPRPASIQEFSAAQSQLREQLPSYMIPAIFIGVSCIPRTPSGKADRRLLRMTAARLSREELQAFAGSPVHSRPPTTATEHALQQLYADVLELPIMRISMEDSFVRLGGDSIMAVRLVGAARQAGLVLDIRDVLGTARLEEQARKATPVSEETPCEAYVPFSMLGSRCTDRDEVLRVAAEQCGTSPSEIEDIFPCTPLQEGMLALSSSQPQMYVGQIVFGMPEDVDVSQFKAAWQSTADATPILRTRIIHTPQGLLQVVLRGKLAWENYNEHPEACAADVGSQIGSPGAPLIRFALGDGDHRGEFTLTVHHAVWDAWTMRLIHDALERSFQGEMTKKHPFHPFIQYLQQVDGATMDDFWRTELADLEAPSFPALPSTQHRPSPTAMLRHTVEKIEVVPRIHTMASYIHLAWSLLVAHYTDSTEAVYGAIVSGRNSPVAAINELAGPTIATVPVRVRVSPEDTVSAALEQIQTCMVRMVPHEQAGLLRIAKASPDAARACSFQSHLNIQVVEQEHRLLPARRGIASTGMELTRFSSYALNLMLQLSPDNTSVTVDIAYDPQVLSAWEVDRMIHQWEHILRQICREPSGSLQELDFASPQDRDLLRLRNSETPTVDWRCLHDLVLAQEARQPSREAVSAWDGDFTYRELAELSSNFARLLNLFAVGRGSFVPICMDKSRWAIVSILAVLQAGATCVLLDPQHPRQRMQDTIAGLSVPVLVNAPSTAPVTKGLCAIELCVSAKLTEQLWTNAYGSRFQTHVDPDDLAFLIFTSGSTGVPKGIAMPHCTVSSSIYHNSAPMMFDADTRALHFSSYAFDVSIYEIFTTLASGGCVCVPSEFQRTNELADFIQQRAVNWTFLTPSTAQSLHPSEVPGVATLVLGGEAVTPDHVKTWAPGRSLINGYGPAEATICAVGPMPEHGWDPGNIGHVVGGVGWVTIPSDPSRLAAIGAIGELLLEGPFLARGYLNQHEATAASFISPPPWHRTLLPDCDAETTRLYRTGDLVQYEEDGSIRYIGRRDTQLKLRGQRIDLGEIETQLRRSFPGVHDVVAEAIQLPILQDRAALVAFIGCQEAQVTESAVGEQVLSAVDESFQHTVSLAQTRLQAILPPYMLPSVFFPLAHCPKTLTGKTDRRYLRQAVLALPPHELQRYRVAGRQKARIPVSRGPELRLQSIWADLLRIPCDDIGSDDTFLLHGGDSVAAMRMVALARRADFTFRVTDVLSNCTLSELARCTGEEPCLTDGDGTLPTTHEFESGHKMVDSPVSADYHTGMIGTELEMENDAIAVYPTTQAQSFLIKRYPWTHWRFSFHGEVSVERLRTACARLVAAHSIMRTLFVAGAGGERVRHVVMKELDIPLHTGTTHKNLVDYCQSICDAEQEMDVLEAVLPTRLTLISDALQTSHIFILRLSHAQYDGICVPKIFADLEALYNGTEPIAPTRFERYLDERRWYSGERARAFWKEYLAGSSPPCTMPVKATPPTDSDDSRPSAARSVISASQTVRCTAIPFQVTLATVVKAAACLVLARLTGRTDITVGQTVNGRSLPQPWVSEVVGPCVNYIPFRATLSESMSIQDYLVHMQSQHNRCIHYDGAELDTIIKNCTTWDPSTEFGFILQHQNIDMDLSLTLDGNRCASCASSGRLRPSNEVWICSTPSPSGVDLDVVASSQTLTADAAKNLVDDIADMIQTLLYNLETPLRDVVEFD
What is the function of this protein?
Nonribisomal peptide synthase; part of the gene cluster that mediates the biosynthesis of notoamide, a fungal indole alkaloid that belongs to a family of natural products containing a characteristic bicyclo[2. 2. 2]diazaoctane core (PubMed:20722388).  The first step of notoamide biosynthesis involves coupling of L-proline and L-tryptophan by the bimodular NRPS notE, to produce cyclo-L-tryptophan-L-proline called brevianamide F (PubMed:20722388).  The reverse prenyltransferase notF then acts as a deoxybrevianamide E synthase and converts brevianamide F to deoxybrevianamide E via reverse prenylation at C-2 of the indole ring leading to the bicyclo[2. 2. 2]diazaoctane core (PubMed:20722388).  Deoxybrevianamide E is further hydroxylated at C-6 of the indole ring, likely catalyzed by the cytochrome P450 monooxygenase notG, to yield 6-hydroxy-deoxybrevianamide E (Probable).  6-hydroxy-deoxybrevianamide E is a specific substrate of the prenyltransferase notC for normal prenylation at C-7 to produce 6-hydroxy-7-prenyl-deoxybrevianamide, also called notoamide S (PubMed:20722388).  As the proposed pivotal branching point in notoamide biosynthesis, notoamide S can be diverted to notoamide E through an oxidative pyran ring closure putatively catalyzed by either notH cytochrome P450 monooxygenase or the notD FAD-linked oxidoreductase (Probable).  This step would be followed by an indole 2,3-epoxidation-initiated pinacol-like rearrangement catalyzed by the notB FAD-dependent monooxygenase leading to the formation of notoamide C and notoamide D (PubMed:22188465).  On the other hand notoamide S is converted to notoamide T by notH (or notD), a bifunctional oxidase that also functions as the intramolecular Diels-Alderase responsible for generation of (+)-notoamide T (Probable).  To generate antipodal (-)-notoaminide T, notH' (or notD') in Aspergillus versicolor is expected to catalyze a Diels-Alder reaction leading to the opposite stereochemistry (Probable).  The remaining oxidoreductase notD (or notH) likely catalyzes the oxidative pyran ring formation to yield (+)-stephacidin A (Probable).  The FAD-dependent monooxygenase notI is highly similar to notB and is predicted to catalyze a similar conversion from (+)-stephacidin A to (-)-notoamide B via the 2,3-epoxidation of (+)-stephacidin A followed by a pinacol-type rearrangement (Probable).  Finally, it remains unclear which enzyme could be responsible for the final hydroxylation steps leading to notoamide A and sclerotiamide (Probable).
What are the official names of this protein?
Nonribisomal peptide synthase notE, NRPS notE, Notoamide biosynthesis cluster protein E.
What is the protein family that this protein belongs to?
Belongs to the NRP synthetase family.