Protein: TCPP_CLAP2
Sequence: MATVPVSVHDLIRHHAEAHPEALAISKNEDQITYGELYAASTRIAQLLADQGVEKGDVVPLLGSRCLEMIACTLAIFMIGATLVPMEAGSWSEARIQTVLDALEYKTLLVTADGDVRRRKTIDYHEIQRAMTGGNGWDCGNAKVPINGPESVRDVAYIIFTSGTTGNPKGVKVTHQSLLNYVWPAHANAPFNLGVGPSDTSLLLFSVAFDAFYGVLLSTLCNGGHVLLSEPSTFIDDAKKCTLLPATPTLLGTISDVSPYSNVRGIFLGGETPTPDVVRKWWTPSRSMWNAYGPTETTVSVTMAELRPDVPIVLGEPIRNSKIIILDSNLEEATQGEICVLGSTVLALGYYKNQAQTDDKFVLWNNERIYRTGDMAKWTENGLKFLGRKDQLIKNRGFLINLEADVIPAILSQNNVETATVLMHRQRLIAFVTPLTVNGDLVRQEMARRFDQFLVPDEIQSRDQLPQTINGKVDNRALYDELVQRDTVGVASNAPIATADTKLSALMNTMSEALTIPAQMIRPELSFTDVGGNSLLAIKMLSALRQKGLSLSMSSLFLLPTISEISNHIIEFDASVSHDDDDQQAADLAGCGKSLSLPGATRSAREITMTDVQRGMIRSTLHDAPTGYMLITISLHQNARDIHPSRLSNAVSQVLGRHDIFCSSFDLVRGTISVNDRYQHDWETRALDGSPMSQAIADESELLNQRARMSDTSNEFFRPVNAFRLLLGENSESVLLWLVHHALVDGWSVGKLLNDFRAQLLTEHSQAAQQSQFSQYTAALTPHLEKVHEPAELFWRESMAGLLDGTELKVGRLEDGASNGSRIEHECLSLGLSLEQTEIAARALGFSPAVIFHAAWALLLSSYASEDAVVFGSVFSARSFHVPRIEEIVGPLINLCPFPVQVHALGSKMDLLSSVQSLLLQISEYQWSASKILQDIASGSHARIFSTALFLEYDLPLYASSDQHELAAWTYDRKDWPEFGLTLQVQCVGEHLGFRAVIKDPKYESPLASRLLGHFRNLCLFLLSPKISTLAEANDSMLEPTEMLCLTRTSTSLFTPYSGPPTLKQAFEIGVAAWPMSVALESLSGKLLYQELDDITNALACSIRGLIRPRDVVALLSDGSQNWLLGVISIIKAGATYLPLDTKLPAQRMEAMMETSGACLCVYPNASSLAAFSDLSKPRYLVYEHAAVKTMNGSSSDRLEDIVGPDDYAYIMFTSGSTGTPKGIRVTHRATTSHLSFEPARLHARPGRRHAQVFSPGFDVNIAEIFGTLCYGATLVLKDPADPFAHLSRVDAAMITPSFLSVLSPTELQNLDSIYLIGEAVSQSLADRWSPGRVLYNFYGPCECTIAVAYTRLEIGRPVTLGKTIPRVGCYILDRLLRPVPMGVIGEICLYGVQTMEGYIGQNADEVTKRAFVQDPFRRPGERMYRTGDLAFWTENMEMRYVGRADHQVKVRGYRIELEEIENVIRRSDENVSQSVAIVHQDTIYAFATPQGARIDQIQQCLRQHLPSYAVPQLIIALEAFPTTPNQKLDRKALINLLAPVCSRENETTDHTELVVSQVWREVIGLDEEIALSIDDDFLAIGGNSLRQIAAAQKICSKLGCRVPLSLFITSRSIRSLAASVKKHLAQQSLASTTSVSLAEFSNQCQFLSSKLSYLEKEFLRMHKQASNPSSFNVVHRVRLQGDVDSLLLERALRTVVSKHDILRASYVEVDGVPQRVIQTNTIQIDRIECSDDVAKLHDYISTKFELSGVLIRIALVERCGATDVVLVQHHIITDQVSVQIFLTNLSMEYRALACRDESGQTICTSDHSTNDYHVWALWRDSQLEQPPENTHCEFWRSQFGEKTESIRLVQHQKHPAGEFHSVPRRLKRNSSSGSIEVYLAAAALALQKVSRLNTIRLGVPFLDRLEPGTENMMGVFLDALPVCVQIEPHTDLPSLLSTIRTTLTSALAHAIPSFMIKDIVGLDSIFEVMIVYNRFEDRVTRNMSIPGVSISVEAMRAQGAKFPLLIEFNEHVDHVTLEIEYSEDVLTPSSLSRFEQEICNLLDPQIVV
What is the function of this protein?
Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) lacking the reactive thiol group important for toxicity (PubMed:27390873).  Firstly, L-tyrosine is prenylated by tcpD, before undergoing condensation with L-glycine in a reaction catalyzed by the NRPS tcpP leading to the diketopiperazine (DKP) backbone (PubMed:27390873).  Afterwards the alpha-carbon of tyrosine is oxidized by the cytochrome P450 tcpC to form a hydroxyl group (PubMed:27390873).  However, in contrast other ETP biosynthesis pathways studied so far, tcpC is not able to bishydroxylate the DKP at both alpha-carbon positions, but hydroxylates the alpha-carbon of the tyrosine part and the nitrogen of the glycine part (PubMed:27390873).  The next steps involve an alpha,beta-elimination reaction catalyzed by tcpI, a methylation by the methyltransferase tcpN the action of the four enzyme cascade tcpG/K/J/I (PubMed:27390873).  Due to a dysfunctional cytochrome P450 monooxygenase tcpC, the pathway leads to the biosynthesis of probable non-toxic metabolites lacking the reactive thiol group (PubMed:27390873).
What are the official names of this protein?
Nonribosomal peptide synthetase tcpP, NRPS tcpP, Thioclapurine biosynthesis protein P.
What is the protein family that this protein belongs to?
Belongs to the NRP synthetase family.