Protein: SWNK_METRA
Sequence: MSGISDHDYQVLVNEFNATEDISLLGSRLDQLFEQVADRFPDNTAVIHNESEVTFKQLNSSANILARCLAKRGLQQGDVVGLAVSRSIDLIAAMLAVLKLGAAYVPIDPSFPAERINQMVEDAGLRLILLSGRPTKGLGRWASLCLSVSEARDGSVTDGTNLETEIQTRDLAYVIYTSGSTGRPKGVEISHGAAANFLSSLRKHEPGCSERDRLLAITTISFDMSALELLLPLVSGSAMIVANTSAVKDPRELISLMARHKVTILQATPATWTMLLESGWKGNPRLSKVICGGEPLSRQLADRLLAAADSVWNVYGPSETTYGSVGRVGQGDIVVGNPVANGRIYVLDDNMSPVPIGSEGEVYIGGGSVSNGYRNKAELTRSRFLVNPFHGGVFFRTGDLARFIAPGKLQVVGRIDGVVKIRGHRIDVGDIEAVLVDHANVSEAVVVSRDDRLVAYCVLHAPCHDAASLDGILRPWVAERLPAYMLPTFFVQMDALPLSPSEKVNRKALPDPIEAMQHQTMIQPTSELEQRIQAIWSDILGHDRFGIEDNFFRIGGDSVRIIRMQAALEKQLHRPVPTPKLFEHYTIKALAAYLAGTGRENNNESQAVSQTFAGSHEDIAIVSMACRLPGGVATPEALWQLLQSGGDTIIDVPKDRWDADKLYNADANIDGTSYCRRGGFLDAIYSYDASFFGISPREAQAMDPTHNLMLELCWEGFERSGYTRDQLSGSATGVFLGVSNNATTNGTPPDLKGYSITGSASATMCGRLSHTLGLRGPSLAVDTACSSSLVATHLACNALRQGECSMALAGGVSLLTTPGIHIEFSKLGGLSADGRCRAFSDDTDGTGFSEGAAIIVLKRLSDARRDGDDIHAVLRGTAVMHGGSSAGLTAPSGPGQVALLRNALARAALEPGDIDYVEAHGTATKLGDPIEATALAEVFGTERSGSDPLRIGSAKSNLGHTQAAAGVVGLLKVVLSMNHDTLPRTLHVREPMAAVDWKRTNMELVLQNRPWLPNNNRLRRAGVSAFGIGGTNAHVIVEESPPPAVEETGNITLPSLPATLPFVLSGQGDSALRAQAEKLRLHIESGAGKDSPLRDVAYSLATCRSHLHRRLVVMAGDKAETLEKLASVSSGPTQPLSVNEVGSPTVAMLFTGQGSQLPGMGKDLYAVYPVFRDALDEIAAKFTDLERPLLDIMWAESGSENAALLSRTDFAQPALFALEVSLWKLWQSWGVKPDFLLGHSVGELAAAHAAGVLDLSDACRLVMMRGRLMQAIPRQGKMASVEASSAEVSAAIQELGQHDKVEIAGYNTPLQTVISGHEEAVEATSVYMSKLGRKTKLLDTSHAFHSFHMNGMLDDLRALAQNIRFSPPKMRIISSMTGRLAGAGELERPEYWAQQARNAVRFSDAFQTLAGQGANVFLELGPSAMLCGLGAACLADPGQVGAALWLPSLKPNMNGPLVIQSSLSELHVRHVPVDWAAYFKPFDCKRVMLPTYAFQREDFRPANKASWFDVASLSTGTNDAAPRVQDMMFEINWRRVETKSIQPRGAWGLLCPSGETAWTTEAQRALLATGIQLVAVSKPHEADQLDGLLSLWDSDADTVQMAHGVTALALAQLQEAIRTGLGAPIVWVTRHAVGAGADDRPVNIGAGPLWGLMRAARSEHPELRLRLIDVDEETDRASLSQAIMLADQTEIAVRREQLLMPHMERAGLAAPLPVGQPFVRTDGAVLVTGGLGDLGSRVARRLATAHGVCDLVLVSRQGTNSPGADALVAELAELGAKATIVGCDLADLDSLGSVMQLFTPDRPLRGVVHAAGVVDSGTLSSLTPRKCAAVFAPKVDGLWNLHQLTKHMDLDLFMMFSSISGIIGLPGLGNYAAANSFIDSLAHLRRAQGLPASSVAYGTWAGDGMATTLVPTTRAHLSQLGLGFLPPEAGLEIFEHAVYQGRALTVAAVLDLQRLRAYYEEQGGVPPLLNSMLGGTKVQKPADEVVNLRDLLADAAPEQHSSIVLHMVQATIAKALGYTRAEDVDASRPMQELGIDSLTAILTRNHLATLTGMALPPNIALLYPNLKSLSEFLLCRLMDDVESSTSSPSDTDGATPSTPTSAASCVDMADIRRGVLDSTFQFNNPVSPGVPGTVFVTGATGFVGTFMIHEFLQRRISVYCLVRASGSQEAQQRMITTLKQYGLWRPEHEPLLVSVAGDLSQPLLGLGEVVFDDLASRVDAILHSGALVDWMRPLDDYIGPNVLGTHEVLRLASCGRAKAIHFISTISTLPIHAGYGLAEHDGEYGYGTSKYLAEKMVVAARFRGAKASSYRLPFVAASAASGRFRLDRGDFLNNLVTGSLDLGAFPLINADLSSVLPVDYLCGTIAAIMTEDQERVGEDYDFVNPQALTFKHFFEMMCSVSGGKEMVSFGEWHRRALEYAAAHPTSSLARIATVLDGYNDKTVGSLVSGSPVGKHVLGLDAYRAPPLDEEYVRRYVHCIEAARAKMRT
What is the function of this protein?
PKS-NRPS hybrid synthetase; part of the gene cluster that mediates the biosynthesis of swainsonine (SW), a cytotoxic fungal alkaloid and a potential cancer therapy drug (PubMed:28381497, PubMed:32786262).  Swainsonine production occurs via a multibranched pathway and is dispensable for fungal colonization of plants and infection of insect hosts (PubMed:32786262).  The first step of swainsonine biosynthesis is the production of the precursor pipecolic acid (PA) via conversion of L-lysine (Lys) to 1-piperideine-6-carboxylate (P6C) by the aminotransferase swnA, the latter being further reduced to PA by the reductase swnR (PubMed:32786262).  PA can be converted from lysine by both the SW biosynthetic cluster and the unclustered genes such as lysine cyclodeaminase (PubMed:32786262).  The PKS-NRPS hybrid synthetase swnK uptakes and condensates PA and malonyl-CoA with and without skipping of the ketoreductase (KR) domain in order to produce 3 intermediates, 1-oxoindolizidine, (1S)-1-hydroxyindolizin, and (1R)-1-hydroxyindolizine; with the transisomer (1S)-1-hydroxyindolizin being predominant (PubMed:32786262).  The terminal thioester reductase (TE) domain of swnK is involved in reduction of the thioester bond to release the intermediate aldehydes (PubMed:32786262).  The oxidoreductase swnN could contribute to the reduction of 1-oxoindolizidine to (1S)-1-hydroxyindolizin and (1R)-1-hydroxyindolizine, contributing to the major route of SW production (Probable).  The dioxygenase swnH2 would be responsible for the oxidization of (1R)-1-hydroxyindolizine into (1R,2S)-1,2-dihydroxyindolizine and of (1S)-1-hydroxyindolizin to yield both (1R,2S)-1,2-dihydroxyindolizine and (1S,2S)-1,2-dihydroxyindolizine (PubMed:32786262).  The dioxygenase swnH1 then performs the conversion of the 1,2-dihydroxyindolizine epimers to SW (PubMed:32786262).
What are the official names of this protein?
PKS-NRPS hybrid synthetase swnK, Swainsonine biosynthesis gene cluster protein K.
What is the protein family that this protein belongs to?
In the N-terminal section; belongs to the NRP synthetase family.