Protein: BUAA_ASPBU
Sequence: MAPQNEPIAIVGSGCRFPGEASSPSKLWDLLREPRDVLSKIDRFSADGFYNKDGHHHGSSNVLHSYQLSEDTRSFDAQFFNIPASEAESMDPQQRFIMEVVYEALESAAIKIEELSGSPTAVYVGVMCNDYAHITYADLESVPKYAATGTALSILANRISYFFNWTGPSMTIDTACSSSLVALHHAVQTLRGGTSKVAVAAGTNLIFTPTNYIAESNVNMLSPTGRSRMWDANADGYARGEGVAAVVLKTLSQAVADGDRIECVIRETGLNQDGRTPGITMPSSVAQAELIRSTYARAGLDVTRESDRPQFFEAHGTGTKAGDPEEAKAIYKAFFGQEDTMDARDTLYVGSIKTIIGHTEGTAGLAGLLKASLAVQNKTLPPNMHFHTLNPDIEPYYGKLQILTSVKPWPALASGVPRRVSVNSFGFGGANAHAIVESYEPSNGAIKVQSSKETAIPFTFSGYSEKSLMSQLTSFLEYLDSHPEPRLRDSAWTLSRRSAFSTRTTVSGTSIERLQEKLQSKIDSKIKDGKALGIRSSSKNDKILGVFTGQGAQWPRMGLRLLQSSATARRIFDDLERSLAELPTEDRPSWSLVQELEREAEDSRVMEAEFSQVLCTAVQVMLVDLLHSVGVSFDIVVGHSSGEIGAAYSAGYLSARDAIRIAYYRGKFGKLACGRDGVAGGMLAAGTDMADAKDLCELDDFVGRLQLAASNSSSSVTLSGDAEAVAWAQFVLEDEKKFARLLKVDTAYHSYHMQPCAEPYIEAMKRAGIQALKPQSDCRWFSSVLEGQEVSAAMSASLANSYWRDNLLQPVMFSQALEQAVSNTSDIGLVLEVGPHAALRGPATLTINDKLGRDVPYFGLLSRNADDVESFSDGIGAVWASLARCVIDFTRVDALLADGPEDQPRLCDNVPGYSWDHQRTYWMESRSSAALRLRPAAHHELLGVRVDSLNREYRWRNFIKPSQLSWTRGHQVQSQLIFPGAGFSVMALEAAKALAPAEKIALVELTDMQVLRAMAFQDENTAVEVICSLSNVIEDPDQANLTANFTCDMCLSKESGFVMAACGAVRLQLGAASSQLLPERTACPVRMNDVNIEHFYSTLWSLGYNYTDMFRSITSLQRTTDAASGIIHTTTEPDYTTSLTLHPATLDVAFQGIFGAMGAPGDGRLWTVLVPTRIKRITINPAVCGGTSGLGVDLPFDASVSVSPIDGVAGDVDIFDSTGVNKAVQVEGLQVAPLVPVTQSDDREVFSDTIWNFQEPDAARDVPKWTLTDEEWEHARYVERACFYYLKQLHDTITAEERDRCEWHPRKMLDWATEVVGVVSRGEHPIIRQEWMNDTWEMLKGPLDELTAKYEDFESLTHVGKNLIPFVKGEFSLLELVRNGGLLEHIYRNTYAFCEYNNYLANLVKQLSHRFPRMDIFEIGAGTGSTTEAVLRGIGDHYSSYTYTDLSAGFFPNAQETFKEHDAKMIYKIYDAEKEPGKQGYTERTYDLVIASNVLHATHSLETTLTNARKLLKPGGYLVMLEVTDVNPLRPTFFFGCLPGWWVGENDGRPHHPLVTKERWGELFDRTGFSGLDTSTPSHDVFMAPFSVMLTQAVDRQMALIRQPLQEDNRTTIDHLLILGGTGFTSFMLIEDVKHQLKRYAKHVIVVETLEALEASHFHSRQMLLSLVELDAPVFSPFTPERFAALQMLTEKCRNVLWVVRGASGEQPYANMMNGVARCLVGEQPDMRFQFVDFDMADKVDAGFIVRSLLQLQISDAWHTFIEPYRPVWTLEREVRYIQGQAHIPRYSPSLRRNLQYNSWRRTIRETVDPSSKYVILTHANGYYDLEEDNSPRPDPMAEDDRMAINVSRSSTVAVEIDGIGHLYILTGECELSGQRVLAFSSHNASRVQVKKDWVVSIGILSSDEPALIQMVTNVCLGTMLLNQTPRNGSLLVYEPTVALARILTALTSAEEPGRVLFTTTNRAKLDSGVAFSFIHPSSPDSSIARWVPAGVAGFVDASGGRKEQNMAARFARHLSSQCRVISMEGFYSNSSHQWGNAGGNSLSALLQHSTGLFTQGYKQCKQNQVQELSLDDVIGASTEHKEIRILNWKSQSKALVKLSPVQDEITFKGDRTYLLVGLTGELGRSLCRWMVQRGARYVVLTSRKPDVEPAWLELMQSYGAHIEVMAMDATDRKSTYNTVRKVQQTLPPVAGVGNGAMVLNDGLFNVISHQDFNQTLRPKVDGTTYLNELFQSPDLDFFIVFSSLAYVTGNFGQTSYAAANAFMASLVEGRKRRGLPGSVMNLAGIFGLGYITRTDRSILERLGKLGYANISEYDFHQFFAEAVLSGVPGSGRCHEISSALRPIDPDGETNPPAWLDMPRLSYYRHTKHAFTESGDTKSLSVRSQLKEQTTMEDVQRVLTNGLILTLYKQLGLDPEDDAISPDTSLVELGIDSLVAVDMRVWFTKELDLDMPVLKLLGGATVAAMVEDTMERMSPDLIPNVAQKDVTVAADRPSAPSDGVPAVGRSTAVSTTEHNSEEQESHAMETQELDESTTSGGECSSTKESSSSEATPPPSSVMSEDLAKVEETASIDGPKYVRKVKMGYGSLQFFFLVKHLDDPTVLNMQFRLPLQGSIKIPDLKYAVKMLGQRHEALRTAFFVDAENDDEPTQGVLETSPLQLETMQVTDSKEARRVCEDVQKYVFNIESGETIRILLLSITPSSHWLVLSFHHISIDGFSFNILLDEINALYQGQHLPPVKTQFTDVMCKQRQDLQAGFRRSELAYWQQVLGKIPDPIPLFPVAKLSSRLPVTRYHFEEAPMASIDAATAEQIRKQCRALKATRFHFFMTVLRIFLFAFLDTDELCIGFADASRADSGVSRTVGYLVNMLSLKFQRKPSQTFAQKVEEARKQSYAALANSTVPFNALLEKLEPPRSAAYTPVFQVFMDYLQHKFTAPKGLGVVEEQVYAHLTHNFFDLAVDINDVSASEILVRFRMQQYLYSASSVSLLLKSYVQLVKMCAYMEPNKAIGEPVPYDAQDIERAISLGQGPVVSSQWPSTPIERILDVAQARPEAPALVDGEGARLSYMEMIDRAHSIAGCLLTAGVAEGSTVGVYQEPTADSICSLLAIWIIGAVYLPLDRRVPCSRLSSIVQDCQPSAILCHERTLSDTPYLEATKQTAIITVPVNVAGIEAAPVPLNTGNGDQTSIILYTSGSTGVPKGLPIRHVSLLNQIEAMTTTFGVGAEMVLQQSAPSFDVSMQQILMALCNGGALYVVPNETRLDPVTITRLIASEKITWVHATPSEFTQWLRHGSAHLRAAKDWKFAFSSGEALSSDLVKGFEALRRPDVKLINVYGPAEAGVITGTEIDTTHVSAEPRSPISLGSPLANIAVYVVDRNLRPVPVGVSGEIVVAGAGNISGYLNRFELTAKLFLPDTITPRGYYPGQLATLYRSGDIGRYSPDGQLYYEGRIAGDTQVKLNGIRIDLKDVESAILETSGGEIVNAIVTDRRSPDFLVAHVELKADFPEAERKNFLTYIQQCLPLPKYMCPAMFIPLDHVPLNSHGKLDRRAIAARPLPTVDGDDQQGDADLSETELALRELWTGCLPEDVVKLTSISATTDFFHLGGNSYLLVRLQRLIRDRFNVSVPVMALYDASTLMAMALKIRNSQSLAVIDWDTETSVAQSLGASPCAEQPTAPRKTTDLTVVLTGATGYLGSRIMKALIASEQVSQIHCVAVRGHSAGVPRELAHSSDKLILHGGHLEDPLLGMSEEEFTFVARETDLVIHSGANRSFWDHYERLRGPNVLSTKTLVDLALQNQAPLHFISSGGVHLLCSGEDYAAESLASYLPPTDGSNGYIASKWASEVYLEKAAQKTSLPVYVHRLTPAPDVTPDAPMELLEEMSALAVKLQALPAPSGWTGTFDLTPAEALATGIAAAAVGAQAPMLESHQSARFIHHPSQVKMTMDHVAKYLDMLPSAEGFERLPPLQWAGRAKREGLTWHFSSTDFITMGG
What is the function of this protein?
Hybrid PKS-NRPS synthetase; part of the gene cluster that mediates the biosynthesis of burnettramic acids, an unusual class of bolaamphiphilic pyrrolizidinediones that display potent antibacterial, antifungal, and cytotoxic activities (PubMed:30735051).  The first step of the biosynthesis of burnettramic acids is the hydroxylation of proline by the proline hydroxylase buaE to generate 4-hydroxyproline (PubMed:30735051).  The PKS-NRPS buaA and trans-enoyl reductase buaC construct the highly reduced polyketide chain, and the condensation (C) domain of buaA then catalyzes the amide bond formation with the activated 4-hydroxyproline (PubMed:30735051).  This is followed by the R domain releasing the nascent polyketide-peptide directly via a Dieckmann condensation to afford a tetramic acid fused to the hydroxyproline, generating the bicyclic pyrrolidinedione moiety (PubMed:30735051).  The cytochrome P450 monooxygenases buaD and buaG are likely responsible for the multiple hydroxylations on the polyketide chain and its terminus, although in a heterologous context, buaD does not appear to be required.  Therefore, while buaG may be a multifunctional cytochrome P450 monooxygenase, it cannot be ruled out that the two secondary alcohols on the polyketide chain could have an acetate origin (PubMed:30735051).  Finally, the glycosyltransferase buaB transfers beta-D-mannose to the aglycone burnettramic acid A to form burnettramic acid A (PubMed:30735051).  Burnettramic acid B is a minor cis-pyrrolizidine epimer of burnettramic acid A and it is likely that small amounts of it form naturally in acidic environments (PubMed:30735051).
What are the official names of this protein?
Hybrid PKS-NRPS synthetase buaA, Burnettramic acids biosynthesis cluster protein A.
What is the protein family that this protein belongs to?
In the C-terminal section; belongs to the NRP synthetase family.