Protein: CHEA_CHAGB
Sequence: MSDNDDEWNGFSDDNGEDDGPPEPGRPSQIDGQPWDVPDSALLQGDVIPNPKDSDFATDFAKIEELQGAINAWARSRGFAVVRRHGRNKQDGLYTRFDILCDRFGSPRAPRGAGVRETATRKCDCKWKAAASRTKDGWRFHSHPDPQHSVHNHGPSLHPSAHTQHRMSNSESLDTIAELSNHASIRAREIRSVVNQEHDTIYTRKDIYNVRAKMRKVNLDGYTAAGALIKALDNVDGDTANHYEVEWADAAETIFCSLVWGFESCLEATSIYHDCMLIDLTYNTNYMGMPLYQVNCLTSVGKTLSTMFGLVSDETTQTFRWLMKATKKLRDKFNIPEPAVIVTDHCKELKQAISEVFPDSQQQTCIFHVIKNVMLNTKRKFKYPGRDEVDSEDEEYRADFEDYDGVSPQERAAMEKDHAERLLSRNTSTSKVTKPISHDPRGVEEMFKAMQWMSCRDQWAHCYTRHYRNFGVRTTSPTESNNMSIKSYLINGRSSAYSLVSVSQDLCKEQVQQNVEEMAKQAIRARHDFLSRPWLGALPLRVSYKALDLIVGEYRRAKAAMPSTRPSQSIRRPLEPCHPDTCTATIQYSIPCRHDIYKKLAEGERLELREVHTHWHLQMSLVKPPLPSVEDNVADPKIVENRKGRPKNSARPLPDGLGIPSSPKTPRSQRRDGTQGSQGSQGRRTPGSTPGPPRGNAPRLQPSIRRVLSAHETVEEPRPPQAAPKRRGRPPGSKNKKKDQAPAPAESQLSAAERAEPRTTLVIASAPRRGLRAAPAAETTASEKRKAQAGGDSAPAPKRTRATASTPRAQSDQGTGNVAIPSLPAPAGAPGARVTRSGRAVRLTAKAAKAASGGRISYFFDWHGPSITIDTACSSSLIAVHQAVQSLRNGEVPLAIAAGANLLLGPSQYVAESKLKMLSPGGLSRMWDEEADGYARGDGFASVVLKPLKDAIRDGDRIECIIRETGSNQDGRTQGITMPSPIAQSALIRETYQRAGLDLSRPADRPQYFEAHGTGTPAGDPVESEAISTAFFGPDSRFKRQPGDAKLLVGSIKTVLGHTEGTAGLASLIKVSLALQNGKVPPNLHFNRLSPSVKPHYQNLQIPTSLLDWPEVPEGGVRRASVNSFGFGGANAHAILEAYTPATAKELLTAPSPFSFAPLLFSAASDTALAANIAAHADFVEKASDVNLGDIAHTLHSHRSALAKRAVFAAASRSDLVARLRGHAAEDKAKSDAAAPLGRSLSSRPRTLGVFTGQGAQWPRMGAELIERSEAVSRIVDELEASLATLPQQDRPSWSLRAEMLAPAASSQIGKAEFSQPLCTALQIILVDVLREAGVVFDAVVGHSSGEIAAAYAAGVVTASEAMRIAYYRGFHTHRCGGAGGQSGAMMAVGTSFEDAQELCALDAFKGRLSVAASNSSSSITLSGDADAVAEAAVVCEEENKFHRALRVDKAYHSHHMVPCLGPYVESLRGACNISPTPQNGSGSGCVWISSVYATDIADVQDDIGSEYWAKNMGQTVLFSQALETALRERGPFDQVVELGAHPALKGPAMQVIEETTREKIPYVGTLLRQRDATEALAESLGALWAANGRASVDLAKYEAFLSGGRVHQVLADELPRYQWDHQTAYYHDSRLLKALRTSSIKPNELLGTRIMDNSPSEARWRNRLSANEVPWLRDHRVQNQAIFPGAGYIATGLEAVRELLGNEPLLLVNMQDIFIGQALIIPEPGSVETLVSVTNIVRGADKITARFTFFADEGRADSVSMAEKASANLIISLGEPDPDALPPRPEPGRDYHMLDVPAERFYDAVGSLGFGYTGPFRALSGLSRKMDYATGSVVQPEPTEGFGRLLVHPAALDAAVQSIILAYCFPGDTRLRTTHLPTRIDSLRVNIPLCEADRSAQTPFRSSVPSGGGVELSDINGDVDLYDENGSTLIQLQGLHTKPLVPPTPSTDLPLFTEWVWGPLSPHGRDLTLRGAEAEAERDLFNDLERVAYFYLRRLDAAIPPEQRVDLPAHQTALFRIDLELMHAVGENLASVIRGEMNMLEPMMQDNKLNRFYIEALGMSRYLEELSRMAAQISHRYPQMHVLEVGAGTGGATKVLLRHLEGGFESYAYTDISSGFFPSARETFEAYTDKMTFKTLDIEKDIAEQGYQEESFDLVIANLVVHATKDLQVTVRNLRRLVKPGGYLLLLEITDNDPLRFGFIFGGLPGWWLGEEEDRGLSPCVEVATWDRILRNTGFSGADEVTVRDPNNPLSVILTQALDDRVELLRQPLTAQPSSEGVQLDSLTILGAETGRAAELARDVEALLSPYFRRSRTVSSLVGLGPQDLPLMGTVLSLVELDTPVFKGITPERLRGFQQVFQQSKNVLWVTTGYKADDPYSAMVYGVGRNVVLEMSHLRLQFLDLETLAAADPRILAECVLRFEFSDMWEQAADKRPLLWTTEPDLAYEEGRLRMPRIKVSKERNARYNSSRRPVTKEVDPIASPLALQPLDDTGKDYALVAPSGRLAAGTRLDTVKIRVAKSILRAVRVLATNYLFVVAGFAENGTSPLVAVSDSQASVVEVDRAWTMPIQHSEGAADTAVETAMEQAMVALYDGLLAQALLQDVEHGRALAVLDASPSLTRALRSSGRYRGVQVVSLASKAAASGVPGTIPVHPRESVRSLKSKLPAHVDKLANFSDRADLARTVASCLPTRCDFQDWQSLTRPGAVITERTLLGLADCEVPSVLRAAWAHVKVDQRSTDLAGVLRADPTSLSQAAVVPGDAANQVCLVDWARRPTLPARIQPLVTTITFSPDKTYWLVGLTGGLGQSLCRWMVERGARYLVLTSRNPKLDPRWLAGVEALGAVVRAFPNDITSRDAVQAAYRTITATMPPIGGVAHGAMVLHDSMFAEVTVEKMDKVLRPKVDGAIHLDEIFYDAPLDWFVYLSSVVVITGNKGQGIYAAANMFLNSMTMQRRKRGVPAAAVNIGAVLGNGYVTRELNHQQQTFLQEVGNNWLSEQDFLTIFAEGVAASRVDSTEAVEITTGLRMLSSRDENVTWATNPKFQYLVQAHVASAAAKLAKSSNVSLKKQLEDVKTIQDASEILEDAYTSKLRAVLQIAPDRDVLSAALDDLGMDSLVAVEIRSWVLKELSADITVLEVLNSGTAGALFELVKERALASLALLDSGEQPDQVKSPRAAPLDLVSGHGGGDRRPSTVVDVADTSLDQGSSWDSGSLREASNGHDSTILSSTAPSSPISKPAGVDASDLEQTPIPEDSEEPVASSPDAGLARSVPLSFSQARFWFLRHFLPDQSAFNITSVVRMHGRPDMERLARAIKAVGNHHEALRTAFRVGEGNEPVQAVLKETVLVLEHRDISDADDVTPAYEAVQRHVYDLEAGETMRLQVLTLSPTEHFLILGYHHINMDGISFEVLFNDLQKAYRGVEFTPGVAQYPAFSILERDEYRLGKWKTELDFWKAEFAHLPEPLPLLPLSQRASRPAVAQYATLRVERRIPADLSATIKSAARKFGAGVFAFYLAVLKALVVRYVDVDNLCIGLADANRRRAEVLESIGLYLNLVPLNVPCDRTQPFSDALREMHTKYQRAFANARVPFDVLLHELDVPRSSSHPPLFQVFMNYRQGVSEAREFCDCECEGELVSGGQLAYDIAVDVVENPGGETNVMLSVQQSLYNAASAEVLLDSFFSLMEGFAANPISRISKPPLHRQTAIEQAVELANGPILDLAWAPTVSHRIDEMIQAHPDKLALTDGQGTDLTYAQLGASVNGIVQGLREVRASNVVGVLQHPTPAAICSILAVLKAGLTYVPLDPRVGPAKLAAIVGEAKPSCILVDDATEVDIGSFSLDDTAKVRNVASLPPSEDRLPIEAVPAGTAVLHCAAASRRPVSELAVQEGSETILQQTAFSFDISLFQSLLALTTASTLVVAPREVRGDPAALAKLMLIAGVTVTAATPTEYVHLIGHGASQLKQNDKWRLALCGGEKLSDQVVSGFRSLNRPELTLVNDYGPAEATFRCSTTVVPYQEEDGQELARTTPLKTCANSAVYILGDDMKPLPVGLTGEVCVGGAGVGLGYLNNHQLSAQRFIANPYASPAFVARGWTTMHPTGDRGRLSPDGGLILEGRIDGDTQVKLGGIRIELEEVERAVINDSQGAIREAAVSVRTDEASGTEYLVAHAVMQDRADSTPAHVDWLQQLQSRLSLPRYMAPSAIVPVAALPLSVSGKLDRRALRELPVATALTTQTPAEESEGLPAMQHLIKQLWEQVIPNGLLAGRDIGPKTDFFNVGGSSLLLVQLQALLREQFSVAPLVQELFQASTLETMAALVASGSGSSTQQGSDTTPAPIDWEAEAGLLQDAYYNSTTEKKQSGPAVANPPKVVVLTGSTGFLGRHLLERLLRTSHIEKVYCVAVRKQPAELPGIFNDPRVEVFPGDLSLAGLGLSDADTERVFSTADAVLHNGADVSFMKTYISLRPTNVAATQQLAALAQRQGRRIPFHFVSSAAVTQLTPLDEVGEVSVAAYPPAVSSPSSSSSAGGYVAAKWVSERHLEQVAQAHGLPVTIHRPSSILGNDASDVDLMGNLFRYVERLQAVPESRDWKGYFDLISVHTVAAAIVKAVVAAREEEQEEEEGHEKGGPAGRVRYQYEAGEIVYPLSTVADMGELEAAGFVLKTLPLEEWVAQAEGAGLNPLLAAYLKAAAGGGVRWAFPKLVSSTV
What is the function of this protein?
PKS-NRPS hybrid synthetase; part of the gene cluster that mediates the biosynthesis of chaetoglobosin A which has a unique inhibitory activity against actin polymerization in mammalian cells (PubMed:23611317, PubMed:33622536).  Chaetoglobosin A and its intermediates are involved in the morphological differentiation of C. globosum (PubMed:33622536).  The first step of the pathway is the synthesis of prochaetoglobosin I via condensation of one acetyl-CoA, 8 malonyl-CoA, and a L-tryptophan molecule by the PKS-NRPS hybrid synthetase cheA, followed by reduction of backbone double bond to install desired geometry by the enoyl reductase cheB (PubMed:23611317).  Further multiple oxidation steps performed by the cytochrome P450 monooxygenases cheE and cheG, as well as by the FAD-linked oxidoreductase cheF, lead to the formation of chaetoglobosin A (PubMed:23611317).  Depending on the order of action of these reductases, distinct intermediates can be identified (PubMed:23611317).  Within the pathway, the cytochrome P450 monooxygenase cheE catalyzes a stereospecific epoxidation on prochaetoglobosin I, cytoglobosin D, and chaetoglobosin J intermediates (PubMed:23611317).  The FAD-linked oxidoreductase cheF performs dehydrogenation of the C-20 hydroxyl groups in the 20-dihyrochaetoglobosin A and cytoglobosin D intermediates (PubMed:23611317).  Finally, the cytochrome P450 monooxygenase cheG can catalyze the stereospecific dihydroxylation of prochaetoglobosin I and prochaetoglobosin IV at C-19 and C-20, respectively (PubMed:23611317).  The Diels-Alderase cheD may play a role in the post-PKS-NRPS biosynthetic steps catalyzing Diels-Alder cyclization (Probable).
What are the official names of this protein?
PKS-NRPS hybrid synthetase cheA, PKS-NRPS cheA, Chaetoglobosin A biosynthesis cluster protein A, Prochaetoglobin I synthase.
What is the protein family that this protein belongs to?
In the C-terminal section; belongs to the NRP synthetase family.