Protein: EFUA_HORCR
Sequence: MPSYHNTDKTLLGDARQSLQQAVDYSLGCQQPDGHWVAPVMADATFTAQYVFFKHQIPELSLDEDGPEIQRWLLGEQTADGSWTLAPDLPGNLSTTVEAYLALRILGVPKSDQAMLRARDFVVRNGGVEGVRFFTRFFLATFGLVPWTAIPQMPAELILLPTFMFLNIYVLSSWARSTLIPILLVRHHEPVYALPNGQSANNNFLDELWCNPGEKNIPFALPLWDLLRRYQWIEFAFTLLDHILALFGGLRRWPCRHMALKRCTAWLLEHQEESGDWAGFFPPIHGSIWALLLDGFSFQSEVIRLGMEALERLVVIDPKGKWVQSTVSPCWDTALMANALCDAGMSGDTRLAKATQWLRDRQLMVSHGDWRNYANTQQAGGWSFQYFNSFYPDVDDTAVVIMTLIKEDPNCTNSDCVMNGVEWMLGMQSRDGGWGAFDVNNNARWLHKIPFSDMDSLVDPSTSDVTGRILECLGLLLSQRKSPLSPRWRHRLQASSAKAIAFLAKEQESSGAWWGRWGNNYHYGTANVLRGLAWFAQTDPSAQMMCMRTLSWIDETQNADGGWGETLASYVDKSLAGLGRSTAAHTAWALESLLRFRLPSDQAIERGVRWLIDNQQPNVDGYYYGTKWQAGAGQGASWRFDHAYVGTGFPSVLYLGYPYYHHLFPIQALSRYIDKASRQGIETLRIPSSSAVILDRPNVLLMAMGSRGDIQVFLNVTKRLSSCRVRIATHPAHQAKVEGHGFEFYDVGGSPEVFSAALANGHGILRSIIDGRFRELQHLLRSIYRSFWVAALDDVQSHSPLKPESSSRPFIADVVVSGPSTSVHVHAAERAQAPLVIISTQPAIITGDFQSPLTMSRAQFNPSRLWNRISFHMLAFFDWLSFGPSFNRMRANSYQLRSLDLAWALFEFVKVSVPHVCLWSTSLAPKPEDWDNNVIIAGYSSMSDDADDVASKSLQAFLETRQPVVAISFGSATIEDPMKLIKLMSAALVKVGASSVVCRSWDSSFEAEADLPSNVFLVDSIPHGWLLQHVEGFVHHGGAGHTAAGAKAGVSQLVMPQFLDQFFWATKVSEMGLGPTPLPLRELFLDELAPRMEDLLSSKYTKACTNMALQLRGDVDGADVAGDEILRQVEAITTCCIFPELSAHWYCTESDLSLSGAAAASLVSSKEIQWQDLELRPAKDWEQQWRTVRSSSNLVRIWRAIVQLLYGFTTTILALFGWLKGTHGYLDGDRHLIKMEDPLRQARLEQAQFDLHLIHQACDSGSSTSLDAKIIENWKARKAVVIHEVFDDDSGASESSRSSLDGGHADSVLDIEEK
What is the function of this protein?
Terpene cyclase-glycosyl transferase fusion protein; part of the gene cluster that mediates the biosynthesis of enfumafungin, a glycosylated fernene-type triterpenoid with potent antifungal activity, mediated by its interaction with beta-1,3-glucan synthase and the fungal cell wall (PubMed:30051576).  The pathway begins with the terpene cyclase-glycosyl transferase fusion protein that most likely uses 2,3-oxidosqualene as substrate and catalyzes glycosylation immediately after cyclization (Probable).  The fernene glycoside then could be processed by the desaturase efuI which catalyzes isomerization of a double bond established by efuA to form the core structure (Probable).  The latter would then undergo a series of hydroxylations in unknown order at C-2, C-19, C-23 and C-25, which would be catalyzed by two of the three cytochrome P450 monooxygenases efuB, efuG or efuH (Probable).  The hydroxy-group at C-25 becomes oxidized by the dehydrogenase efuE to enable a spontaneous, non-enzymatic hemiacetal formation with C-23 (Probable).  After hydroxylation at C-2, acetylation by the acetyltransferase efuC takes place (Probable).  The final steps in enfumafungin biosynthesis require expansion of the 5-membered ring by lactonization via a Baeyer-Villiger reaction mediated by one of the BGC's cytochrome P450 monooxygenases (efuB, efuG or efuH) followed by ring cleavage (Probable).  This type of reaction would establish a double bond between C-20 and C-21 which could be reduced by the reductase efuL to form the final product (Probable).
What are the official names of this protein?
Enfumafungin synthase efuA, Enfumafungin biosynthesis cluster protein A, Terpene cyclase-glycosyl transferase fusion protein efuA.
What is the protein family that this protein belongs to?
In the C-terminal section; belongs to the glycosyltransferase 28 family.
What are the subcellular locations of this protein?
Membrane; Multi-pass membrane protein.