Protein: APF5_GIBF5
Sequence: MHPDVEQELAYTLLIELMAHQFAYPVRWIETQDHILGEVNAERIIEIGPSPILTNMMKRTIASRFSNSDQALNINRHLMSPDNGNPDIYYKYEPTEEPDLPELGSQSNTVKVSGSPAWEVQRPVTASHIPSGPVDEIEDTKVPVSAILISLLAPKLKKDPRAIPMTGTISNLVGGRSTLSNEIVGDLLAEFPNRVPDKPEEMPLSSLSETLSTSHDGQLGKATSALVMKMVSSRLPGGYSLSNARLYLREKWGLPPRRQDAVFLLALQRQPTSRLLSSTDVDSFLDANAAAYFGQENLSIPSRGSVQSAAPAVDAKALLLAKQQNDALLRDIMGVIQGHTAAKNESQDATLQASDSEAAATKKLDMWISEHGDDYAKGMAPIFDAKKQRIYDSYWNWNAQDTILLFQRKLQGQMSSVKELEQLSTSIVNRACGRTLEQLDYIIAQAERDPTVDSGLLKPMQLLRQTCVETQERQPVFINLSPDMAPLTTISSDGRLIFSEIPRALPCSKILSTNEASSIAFPVSRADGVAVSYSPQLTEILCHDLKDSRRSGFSFSGKNVLLTGAGEGSIGNHILRHLLAGGARVTVTTSSFSEKVTAMFQSIYARHGSKGSVLRVVPFNQGSHGDVQNLVKYIYADASWDLDFILPFAAISENGRDIEDLDSKSEIAHRAMLTNLVRLVGAVASQKRQRDTITRPATVVLPLSPNHGLLGNDGLYSESKRSLETLIPKWSSETWGSYIALAGVIIGWTRGTGLMDSNDVIAQAVESLGVRTFSAVEMAANIVSVMGGRFNAECQETPIIVDMGGGLGSVKNFKAKLTSARQELNAYAELKRLTAQESSRDKAYVATDAHAKTSKRLRGRANILLPLPKNLNYEVDIAPFAASLDGMVDLSRVVVITGFAELGPLGNSRTRWEMEESGTLSLEGCVEMAWLMGLITYHNGIDKKGDHYSGWVDTKTSDAICDDEIPGKYLEFMAKHSGIREVEPEISDNGYDPSKKESLIEVALQRDLAPFETSIETAETLKRQHGDKAIVTQDASSGNCQVQLKAGAVVMVPRASRFNRTVAGQIPLGWTAKRYGISDDIIEQVDPVTLFSLVCTVEALLCSGIIDPYEFYQHIHVSQFANCLGSSMGGLTSLRKMHRDRYLDRSVKSDLVQETFINTTGAWINMLLSSSSGPIRTPVGACASSLESLDTACDLIMLKKAKVCLVGGFEDFVEDLSYEFGCMKATCDTDAEYAAGRVPKEMSRPTASSRSGFVESQGCGVQVLTSAELALEMGLPIFGIVAYTSMAADKAGRSVPAPGKGVLTNAREKSTIPNPMLSLGYRKRLLELRRTQIYQNISDHLDILNSEIALFQESETASPGDLKAFRENRELRIRQDAQAQDAEVTFSLGNQFWKSQDAGDISPIRGSLAVWGLGIDDISVASLHGTSTVQNDLNETMVIQEQMKHLGRRQGNLLPCVCQKWLTGHSKGAAGAWMVNGCLQMMNTGLVPGNRNADNVDEKLREHRHLAFPNTNLQMDDIKACSVTSFGFGQKGGQALLVHPRYLFATIGRERYDGYISKRDKRWRKACFRLSEAMVQGNMVSKCIKTEAPYTSADGVAALLDPTARF
What is the function of this protein?
Fatty acid synthase; part of the gene cluster that mediates the biosynthesis of the cyclic tetrapeptide apicidin F (APF) (PubMed:25058475).  The non-ribosomal peptide synthetase apf1 incorporates four different amino acids to produce apicidin F: L-phenylalanine, D-pipecolic acid (D-pip), N-methoxy-L-tryptophan and L-2-aminooctanedioic acid (PubMed:25058475).  L-Phenylalanine is the only proteinogenic amino acid directly used by apf1 (PubMed:24195442, PubMed:25058475).  The 3 other apf1 substrates are non-proteinogenic and have to be modified by other enzymes of the cluster (PubMed:25058475).  Lysine is converted to delta-1-pyrroline-5-carboxylate (P5C) which is reduced to L-pipecolic acid (L-pip) by apf3 (PubMed:25058475).  L-pip is epimerized to D-pip, probably by apf1 activity, prior to incorporation (PubMed:25058475).  L-Tryptophan is N-oxidyzed by one of the cytochrome P450 monooxygenases (apf7 or apf8), and further methylated at the hydroxy group by the O-methyltransferase apf6 to yield N-methoxy-L-tryptophan (PubMed:25058475).  The synthesis of the fourth apf1 substrate is more complex (PubMed:25058475).  The fatty acid synthase apf5 is involved in the synthesis of the octanoic acid backbone of L-2-aminooctanedioic acid by fixing one acetyl-CoA unit and three malonyl-CoA units (PubMed:25058475).  Then one of the cytochrome P450 monooxygenases (apf7 or apf8) may oxidize this backbone to 2-oxooctanoic acid (PubMed:25058475).  The aminotransferase apf4 is predicted to catalyze the exchange of the keto group with an amino group (PubMed:25058475).  The next step would be the oxidation of 2-aminooctanoic acid by one of the cytochrome P450 monooxygenases (apf7 or apf8).  The last step is the oxidation of 2-amino-8-hydroxyoctanoic acid to 2-aminooctanedioic acid is catalyzed by the FAD-dependent monooxygenase apf9 (PubMed:25058475).
What are the official names of this protein?
Fatty acid synthase apf5, Apicidin F synthesis protein 5.
What is the protein family that this protein belongs to?
Belongs to the thiolase-like superfamily. Fungal fatty acid synthetase subunit alpha family.