Protein: POL_SIVEK
Sequence: MGARASVLTGGKLDQWEKIYLRPGGKKKYMMKHLVWASRELERFACNPGLMDTAEGCAQLLRQLEPALKTGSEGLRSLFNTLAVLYCVHNNIKVQNTQEALEKLREKMKAEQKEPEPEQAAGAAAAPESSISRNYPLVQNAQGQMVHQPLSPRTLNAWVKVVEEKAFNPEVIPMFMALSEGATPQDLNTMLNTVGGHQAAMQMLKEVINEEAAEWDRGHPVHMGPIPPGQVREPRGSDIAGTTSTLAEQVAWMTANPPVPVGDIYRRWIVLGLNKIVRMYSPASILDIKQGPKETFRDYVDRFYKTLRAEQATQEVKNWMTETLLVQNANPDCKNILRALGPGASLEEMMTACQGVGGPAHKARVLAEAMTQAQTATSVFMQRGNFKGIRKTIKCFNCGKEGHLARNCKAPRKKGCWKCGQEGHQMKDCRSGERQFFREGLASLQREARKFPPDNNKERANSPSNRELWVSGGEDHTGDREGRKGEDRELSVPTLNFPQITLWQRPILTVKIGGEIKEALLDTGADDTVIEEIQLEGKWKPKMIGGIGGFIKVKQYDNVIIEIQGKKAVGTVLVGPTPVNIIGRNFLTQIGCTLNFPISPIETIPVKLKPGMDGPRVKQWPLTEEKIKALTEICTEMEKEGKISRIGPENPYNTPIFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSCPLDENFRKYTAFTIPSVNNETPGIRYQYNVLPQGWKGSPAIFQSTMTKILEPFRKNNPELVIYQYMDDLYVGSDLEITQHREAVERLRSHLLTWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQTIQLPEKDTWTVNDIQQLVGKLNWASQIYPGIKVKQLCKLIRGAKALTEVVTLTREAELELAENREILKEPVHGAYYNPDKELIAEIQKQGQGQWTYQIYQDLHKNLKTGKYAKMRSTHTNDIRQLTEVVQKVALESIVIWGKTPKFRLPVQKEVWETWWTEYWQATWIPDWEFVNTPPLVKLWYQLETEPISGAETYYVDGAANRETKLGKAGFVTDRGRQKVTSISETTNQQAELQAVLMALQDAGQEVNIVTDSQYVLGIIHSQPDKSESELVNQIIEELIKKERIYLSWVPAHKGIGGNEQIDKLVSTGIRKVLFLDGIDKAQEEHERYHSNWKAMASDFNLPPIVAKEIVASCDKCQLKGEAIHGQINCSPGVWQLDCTHLEGKIILVAVHVASGYLEAEVIPAETGQETAYFILKLAGRWPVKVIHTDNGSNFTSATVKAACWWANIQQEFGIPYNPQSQGAVESMNKELKKIIGQIRDQAEHLKTAVQMAVFIHNFKRKGGIGGYTAGERIIDIIATDIQTTKLQTQILKVQNFRVYYRDSREPTWKGPAKLLWKGEGAVVIQDNGDIKVVPRRKAKIIRDYGKQMAGDGCVASGQDESQDME
What is the function of this protein?
Integrase catalyzes viral DNA integration into the host chromosome, by performing a series of DNA cutting and joining reactions.  This enzyme activity takes place after virion entry into a cell and reverse transcription of the RNA genome in dsDNA.  The first step in the integration process is 3' processing.  This step requires a complex comprising the viral genome, matrix protein, Vpr and integrase.  This complex is called the pre-integration complex (PIC).  The integrase protein removes 2 nucleotides from each 3' end of the viral DNA, leaving recessed CA OH's at the 3' ends.  In the second step, the PIC enters cell nucleus.  This process is mediated through integrase and Vpr proteins, and allows the virus to infect a non dividing cell.  This ability to enter the nucleus is specific of lentiviruses, other retroviruses cannot and rely on cell division to access cell chromosomes.  In the third step, termed strand transfer, the integrase protein joins the previously processed 3' ends to the 5' ends of strands of target cellular DNA at the site of integration.  The 5'-ends are produced by integrase-catalyzed staggered cuts, 5 bp apart.  A Y-shaped, gapped, recombination intermediate results, with the 5'-ends of the viral DNA strands and the 3' ends of target DNA strands remaining unjoined, flanking a gap of 5 bp.  The last step is viral DNA integration into host chromosome.  This involves host DNA repair synthesis in which the 5 bp gaps between the unjoined strands are filled in and then ligated.  Since this process occurs at both cuts flanking the SIV genome, a 5 bp duplication of host DNA is produced at the ends of SIV integration.  Alternatively, Integrase may catalyze the excision of viral DNA just after strand transfer, this is termed disintegration (By similarity).
What are the official names of this protein?
Gag-Pol polyprotein, Pr160Gag-Pol.
What are the subcellular locations of this protein?
[Matrix protein p17]: Virion. Host nucleus. Host cytoplasm. Host cell membrane; Lipid-anchor. Note=Following virus entry, the nuclear localization signal (NLS) of the matrix protein participates with Vpr to the nuclear localization of the viral genome. During virus production, the nuclear export activity of the matrix protein counteracts the NLS to maintain the Gag and Gag-Pol polyproteins in the cytoplasm, thereby directing unspliced RNA to the plasma membrane (By similarity). SUBCELLULAR LOCATION: [Capsid protein p24]: Virion. SUBCELLULAR LOCATION: [Nucleocapsid protein p7]: Virion. SUBCELLULAR LOCATION: [Reverse transcriptase/ribonuclease H]: Virion. SUBCELLULAR LOCATION: [Integrase]: Virion. Host nucleus. Host cytoplasm. Note=Nuclear at initial phase, cytoplasmic at assembly.