Protein: FFSA_ASPFV
Sequence: MATTTAPTTQGHNQPSREPIAIVGSACRFPGGASSPSKLWKLLEHPRDVLKEIPPDRFSVDGFYHPDNMHHGTSNVRHSYILDDDIRVFDAQFFGIKPVEANSIDPQQRLLMETVYEGIESAGLQLNKMKGSQTGVYVGLMSNDYADMLGNDQESFPTYFATGTARSIVSNRVSYFFDWHGPSMTIDTACSSSLVAMHQAVQYLRSGDGSDVAIAAGTNILLNPDQYIAESKLKMLSPDGRSQMWDEKANGYARGDGIAVVVLKTLSQALRDGDHIECIVRETHINQDGKTKGITMPSATAQTALIRSTYKNAGLDITKPSDRPQFFEAHGTGTPAGDPIEAEAIHTAFFGYKGLSKEIEPLSVGSIKTIIGHTEGTAGLAAVLKASLALQAGVIPPNLLFDKVNPKVKPFYGNLQIQTQAKSWPSLAPGAVRRASVNSFGFGGANAHAILEAYEPSSTPTVGTPANVFTALNVSAMSETALRGTLKKYVEYLKEEPSVDLRSLALTVNTRRSTFPVRTSVFGTSVEELSQRLSERSEAEGKTLTPVAPTSLSSSPKILGVFTGQGAQWKQMGAVLLATSPRVVAILDQLEKSLAELPDGPSWSIKGEILADEDSRVNEAVISQPLCTAVQIVLVDLLQSAGVQFHTVVGHSSGEIGAAYAAGYLSASDAIRIAYYRGLHLYLAQGPNGQQGAMMAVGTSFEDAQELCDLPAFRGRISIAASNSSASVTLSGDLNAIEWAKDVFDDEKKFARLLKVDKAYHSHHMLACSDAYRKSMTDCGITVLQPARNGTTWISSVYGEDALDYRHEMNAEYWISNMVSPVLFSQAIEFAMADQGPFDIGIECGPHPALKGPALQVIQEMLGSSIPYTGLLSRGRPDTQALAEGISYLWQALGADVVNYTSFDRFIAGPDASEPQVLANLPSYAWDHDRAFWHESRQYWANRTKEDPPHEILGTKCPDGTDQQHRWRNMLRPREIPWIAGHQIQEQMVFPAAGYVSAAIEAVQMVTRGQSLGAIEIEDFVIGQAIAFNDEYASVETQFTLTDISVEKDIWSASFFFYSASPKSSRSLDLNASGKLKVTLGEPKDDFLPPHLSPEFNMIDVDSERFYDALKKLGFGYTGPFKALGSLKRRMGVATGTITNPTSTDPAHDLLLHPATFDNAIQSIILAYCYPNDGRLWSVQLPTGIKKIKINPVLCNRYAGKKALLCFKASTSDDRSAEIGGDVDIYDEQGNNALMQLEGLQTKPLANATAANDSPLFLETIWDIESPSREAAVADRPDMQPKTELSFDVERVAFFYLRHLDSVATREEREKAESHHKIFFEYIDHTVANVKSGTAQFAKREWMYDTHDEILDIISKYPDSLDMKLMHAVGEHLLPVIRGETTMLEYMREDNLLNDFYVHAIGFDEYTENLAQQVSQFSHRYPHMNILEIGAGTGGATKRIFSKLGKRFGSYTYTDISAGFFEKTRETFREYEHMMTFKALNIEKDPVEQGFTEQSYDLVVASLVLHATHEMETTMRNVRRLLKPGGYLIMLELGDYIEMRTGLIFGSLPGWWMGYDDGRKLSPCMSEEDWSVCMQKTGFSGVDAIVPRQSELPISLAVLTGQAVDDHVNFLRDPLTPGSIDFVESNLTIIGGTTSKVSAMVEEAAKSLGRFYEKIVTATSLAELDTTEVPFMGSVLFLTDLDEPIFENVTEDALTALKQLFKQSRTCLWVTQGARDDNPFQNMSVGLGRVVKLEMTHLRLQSLDFDVETEPSAPTIMQRLLQFEAMAQWEQSGESKDLLWSVEPEIGYDHGKAIIPRLMPNPVRNARYNSSRRLITKYMEPTSANLSLRWSGKSYDIHEGEPSGATSLVMDGRVQLEVSHSTLDAIGVTATDYAYLVLGKNVKSQQQVIALTPKSDSIVRVFDSWTVPYSMAEDDALRLLPVVYTNLMALSVISRLSSGETLVLVEPEEAFAQTLSRLATERAISVVTLTSRMDVKNSDWIYLHVNSPKRLVRSTVPRNASWVIADRDQGGLAANVLQCLPANCKILATESLTSKQPKLDTFSSMAFIPSILRTAFVRAHDIKATLELPSVVAAADISSDNQPSTEATFFSWTASPSVPVQVTPVDHGTLFSSEKTYWLVGLSGGLGLSLCDWMVKHGAKYIVITSRNPQVDARWEQHMKAQGAVVRVYANDITDRESVSSVCKKIRDELPPVGGIAQGAMVLADTMFVDMDLPRVQKVVGPKVNGSIHLHEMFVEVDLEFFVFFSSMAYVTGNQGQSIYAAANAYMTALAAQRRKRGLAGSAINIGTIIGNGYVTRQLTIAQQEYLTHMGNVFMSEQDFHQIFAEAVVAGRPTSKDIPEIMTGLRLAHLDDSDKVTWFHNPKFSHCVLWPEEQGGKAVMSKQNVTVRAQLLLATTADEAREIIEESLAAKLRSSLQIDATVSVINMNADQLGLDSLVAVDIRSWFIKELNVEMPVLKILGGYTVAEMVAAAQEKLSPSLIPNLGKEVDPSLKAVVKAQVEKPVAAAEEKPIVTEKAEYADFDDENEEEGIPTEDSLPEITVSDESSELSDREPAKFNFNGPGFKKVGFSPGPQTPLSEDDRSKWSSYGSPFDSDSDNASIRKSRTSAATSVTALDEYFSKPDHTIFERTLPMSFGQTRFWFLKFYMEDQTTFNITTSISLAGKLDVGRFSRAVHHLGRRHEALRTAFFTDSNNQPMQAVLKEPVLRLEHARGEANVASEYRRIKNHQYDIGRGETMKITLLSLSEKLHQLIIGYHHINMDGISLEVIIRDLQQLYDGKSLAPVSIQYPDFSIMQYKEHSSGQWDDELTFWKSEFADIPEPLPILPPSTKAVRTPLSIYSSNTVKFEVGAELSSQIENACKRTKTSPFNFYLATFKVLLYRLAEGKATDICIGMADGGRNNDLVSQSVGFFLNLLPLRFKQQSSQMFSDALKEARSKVITALANSKVPFDVLLNEVNAPRTATLSPLFQAFINYRQGVQEKRQFCGCESEATKFDGSQTAYDLSLDILGNPGSGIVYLAGQSSLYSQSDVETIAQSYYALLKAFAKNPALRISRPSLYDPQAVDHALAKGKGPTNVGTWPETLVHRVDEIVKAHGSKVALKSATAKLTYTQMAERVNAIASTLQSNGINKCSRVGVFQDPSTDFFCTILAVLRIGAVFVPLEPRLTAPRLATMVQDSDLNAIVYDKANQKTLAELGSNSKKINVSLVLAKSSAVVSNQATPGATAIILYTSGSTGKPKGILLSHSAWRNQIESSSRAWEVPTGTGVHLQQSSWSFDISISQTFVALANGASLIITPKTMRGDSSAITKTIVSDQVTHVQATPSELSSWLRFGDLAALRASKWQFAMTGGERMTPALIDGFRKLAKNDLKLFNAYGPAETTLAVGSSEVDYMTSDDLDTPFTLFPNYSVYILDGQKQPVPAGIPGEVYIGGAGVAQGYLNQDSLTAKRFLPDTFGTTEYTHFGWTKMHRSGDRGHLSEDGHLVLEGRIDGDTQVKLRGIRIDLQDIESAMVQQANGALTEAVVSVCKLQETEYLVAHVVISPTFTGNTESFLDQLRASLPVPQYMQPAIAVTLDALPVNHSGKVDRKAIAALPILPKATQPGATSQPRDSTEKLKDIWTQVLGQGMTSLHHIDAQSDFFHVGGSSLALVEVQAKIKTIFQVEVSLVQLFENPTLGAMARMVDPTAFSAPVNANLTIPAEVATAISAPTTSINTAPKEIDWEEETALTDDFYDIEIDPTPKDQGLPYKTVVITGATGFLGKALLRRMLDDNHIDKIHAITLRRSRSDLPGIFSDPKVHLHRGDLNAPRLGLSETAAAEIFAETDAVIHNGADVSFMKTYRTLSKTNVGSTRELVKLCLPHRIPIHYISSASVVHLSGLESYGEASVSSFEPPQDGTDGYTASKWASERFLERVSEKFSVPIWIHRPSSITGEDAPTLDLMTNMLSFSKKLRKAPTSPAWQGTLDFVDVEKVATEIVEEVKNDSAHPGGLVKYMYESGDLEIAVDDMKGSLERETGQAFQTLSLEEWTKAAAEEVTNELGICCSK
What is the function of this protein?
Hybrid PKS-NRPS synthetase; part of the gene cluster that mediates the biosynthesis of the cytotoxic leucine-containing cytochalasans, including aspochalasin C, aspochalasin E, TMC-169, flavichalasine F, aspergillin PZ, aspochalasin M and flavichalasine G (PubMed:32913332).  The first step in the pathway is catalyzed by the hybrid PKS-NRPS ffsA that utilizes 8 units of malonyl-CoA to iteratively assemble the octaketide chain before addition of L-leucine by the C-terminal NRPS modules (PubMed:32913332).  Because ffsA lacks a designated enoylreductase (ER) domain, the required activity is provided the enoyl reductase fssC (Probable).  The methyltransferase (MT) domain of ffsA catalyzes the alpha-methylation at C10 and C14 using S-adenosyl-L-methionine as the methyl-donating cosubstrate (Probable).  Reduction by the hydrolyase ffsE, followed by dehydration and intra-molecular Diels-Alder cyclization by the Diels-Alderase ffsF then yield the required isoindolone-fused macrocycle (By similarity).  A number of oxidative steps catalyzed by the tailoring cytochrome P450 monooxygenase ffsD, the FAD-linked oxidoreductase ffsJ and the short-chain dehydrogenase/reductase ffsI, are further required to afford the final products (Probable).
What are the official names of this protein?
Polyketide synthase-nonribosomal peptide synthetase ffsA, PKS-NRPS ffsA, Cytochalasans biosynthesis cluster protein ffsA.
What is the protein family that this protein belongs to?
In the C-terminal section; belongs to the NRP synthetase family.