Protein: SEP1_CAEEL
Sequence: MKITNKSVDKQHIEKLDELRKNVSCTVIGFAEQTAELQQEISELFIAEFGVNGPIDMNSLSKLARITSYYASSEYFQGLAKYQRTACKMFITWQTLRKEAMECRSKDREIFASIPAKLCFFYFYNGELCRAVVCLLDYIDLSDDTLAKEAALRWLMFLGETELIEKKLKTWKMDKSSKDMFSATEFAMNYLKKSEYRVEMLEKLMKLRDKVKSDPTRSFSRYELASYVSWLCSTLSNVPVGSALRECEFPDRVSHIQEAALKSDSLVRNRIPGLASSQFDNSVNASIWPFLDGHQEDSNYYVHIGSTIAWHFEMRRECALVNVTTAQTRDSMSAMILNLRVALKSASFFRVLQTTNTLAYYSSIIEEAGSEKNAKLMRVSCVNLLSSNPIIVRCSTPKETGATSRAHTPMAGSSVSEKQNTMRPDLADLLGDLELLDEQSFHPITRSCVCNVCTIYPLHSSFAAEYMMSYAIHSDFSQLSIKHFNDEFARIRERGMSSQVLMHRDSSVRPRPNIIQNEIFGMCVIRWLTKKLDSKESADEDTMEIFNNALKIVRYLQQRTTDMILAVTQLGRQLEFPMECNYSWMRPTIRKPRVKATIDCAVDILRAVSPFGRRPKVEKLEKNLQPFDKERFEKVRLAMRNEMNHYGHILYREWRCRLFAYVGRTSRDPWEAAYAWAESTQIGARNAVQSRLEKCKRGLVTMSGHDRFKTCVQSMPDEMTLVQIAMADDKTIYLVKLHADRDPIIMPLAHYSQAVELMDKFTFLLDEDEMIAKYPGDITPEEFWKRRKIVDGRMMTFVDEVQKHFLGVAASLLMPSGQLGPKAAELAIKIHKLSKGGLLLGEAKEMVYQSKLMDAKSWEALILRFCEMRTTDEKFKSFLPLMHRNSVEVMNQDDSIVTEKKYTYLVICPHLSQFCWERLPIFDEYPYVGRQVSIHSTFSQLEAMKSQEKQIPLQIDVQNAYYILDPDNNLGETQKRMVEYINKFNWEGTVGSAPKSNEISAALSQRDAFFFIGHGSGSSVMPRSVLKQSTCNAISLLMGCGSVRTIPQALGFDGKTAILDYAMAKCPLIVGCLWTVTDGEIDRFLIRMIDDCFEDSKSLTGIDKLRQLSEAMHEARSKARLKYLTGAAVVMYGLPVVAKQTTPFVEKDQRNLPQTPKTSARTSMRMETVPKTPKQEFVTSKSVPMTPIFSNNENKSPSRARMPSRVLKTPRQVKTFQEEDDEAPKRSTTRQLKPLVAPPIPATPTTRTTRSSARTPSRSRNL
What is the function of this protein?
Cysteine protease, which plays a central role in homologous chromosome separation during meiosis I and in sister chromatid separation during embryonic mitosis (PubMed:11728305, PubMed:12498686, PubMed:20116245, PubMed:21878498).  Promotes chromosome/sister chromatid segregation by cleaving the scc-1 (mitosis) and rec-8 (meiosis) subunits of the cohesin complex at the onset of anaphase (Probable).  May cleave histone H3-like protein cpar-1 during meiosis I metaphase-anaphase transition (PubMed:25919583).  Promotes cortical granule exocytosis after oocyte fertilization during the first meiotic anaphase (PubMed:17913784, PubMed:21878498).  Essential for embryonic cytokinesis by regulating rab-11-positive vesicle trafficking at the plasma membrane at the cleavage furrow and midbody (PubMed:20116245).  Regulates centriole segregation during spermatocyte meiosis (PubMed:23401519).  Required for embryonic anterior-posterior axis formation (PubMed:11832245).
What are the official names of this protein?
Separin homolog sep-1, Separase.
What are the subcellular locations of this protein?
Cytoplasm. Chromosome. Cytoplasmic granule. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cleavage furrow. Midbody. Note=Before ovulation, accumulates on chromosomes and cortical filaments in oocytes (PubMed:17913784). By metaphase I, dissociates from filaments and transiently localizes to cortical granules until their exocytosis during anaphase I followed by an accumulation at the cortex near the polar body (PubMed:17913784). Localizes to meiotic spindle matrix (PubMed:21878498). During mitotic metaphase and anaphase, localizes to chromosomes, centrosomes and the spindle matrix (PubMed:17913784, PubMed:20116245, PubMed:21878498). During spermatogenesis, localizes to the spermatocyte cytoplasm throughout meiotic prophase I (PubMed:23401519). At the diplotene stage of prophase I accumulates at low levels in puncta around the nuclear envelope (PubMed:23401519). At diakinesis, transiently localizes to centrosomes until nuclear envelope breakdown (PubMed:23401519). At metaphase I and II becomes highly enriched around chromosomes (PubMed:23401519). At anaphase I and II, chromosomal localization is reduced and centrosome localization is highly increased (PubMed:23401519). Localizes to residual body during the budding division leading to spermatid formation (PubMed:23401519).