Protein: WAPA_BACSU
Sequence: MKKRKRRNFKRFIAAFLVLALMISLVPADVLAKSTEEENGNRIAADDPEETLQKEQTEEAVPFDPKDINKEGEITSERTENTKLYYEGDGVYKQEVYLDPIHTKETPDADWEDISPELKESTSKQVETENAILNSDFQKQMKNGLYATFEHNDHKVTYSLAEAKGPNKTSLTPKDTSADYKTDSNEIVYPDVFPNIDLQTFTFNENIKEDLVLHQYNGYNTFTFQLKTDLQAKEQEDGSIDFSDEKGKVVFSVPKPFMTDSKLDELSGEVERSDKVSYKLEKNEEGYLLHLTADENWLKDPERVYPVSIDPSTSLSVSSDTFVMSAYPTTNYSASSQKWDANLKAYVLKTGYYDKTTGTNYAFMKFNNLKPIQNMTVTKATLKTYVAHSYYGTKATGLWLDTVNSNYDNAKVTWNTKPASKNIGKADVHKGQWASYDVTAAVKSWNSGGANYGFKLHTNGNGKEYWKKLISSANSANKPYIEVTYTIPKGNTPTIKAYHNGDSTGYFDISWKKVEGAKGYKVWIYNGKEYQAISAGNVTSWSTKGKKIWPTSAEIASKRYKLHLDGKDGAELALDPSPVYKNSGGSYATSKNYWIGVSAIFDQGEGAMSAPAKPVIPNVGKAQAPSAKGYNNGNATGYFDLSWKAVSGATGYKVQVFNGKGFETLDLGNQTSWTTKGKKIWPTSAEIKAGKYALHLKDGSGAELPINPGPTYKNAGGDGAKRNYSFKIIAYNKDGEAIASPAATPALPDIARPKNVTGYLYTNTKSSQTGYVNLIWEKVQNAKGYKVNIYNGKEYQSFDVGDADHWTTQNKNIWPTSEEIKAGSYKLHTDGKGGELALDPSPVYNNANGNYKGKKNYSFTLVAYDANGETIPTAPFNPTFHEGAEFLGTEEYWSIIDIPSGQLNGATGNVIVNEEDLSIDGRGPGLGLSRTYNSLDSSDHLFGQGWYADAETSVISTDGGAMYIDEDATTHRFTKKADGTYQPPTGVYLELTETADQFILKTKDQTNAYFNKKGGKLQKVVDGHNNATVYTYNDKNQLTAITDASGRKLTFTYDENGHVTSITGPKNKKVTYSYENDLLKKVTDTDGTVTSYDYDSEGRLVKQYSANSTEAKPVFTEYQYSGHRLEKAINAKKETYVYSYDADKKTLLMTQPNGRKVQYGYNEAGNPIQVIDDAEGLKITTNTKYEGNNVVEDVDPNDVGTGKATESYQYDKDGNVTSVKDAYGTETYEYNKNNDVTKMKDTEGNVTDIAYDGLDAVSETDQSGKSSSAAVYDKYGNQIQSSKDLSASTNILKDGSFEAQKSGWNLTASKDSGKISVIADKSGVLSGSKALEVLSQSTSAGTDHGYSSATQTVELEPNTTYTLSGKIKTDLAKSRAYFNIDLRDKDQKRIQWIHNEYSALAGKNDWTKRQITFTTPANAGKAVVYMEVDHKDKDGKGKAWFDEVQLEKGEVSSSYNPVQNSSFTSATENWNVSGASVDSEEGFNDDVSLKAARTSASQAGSVTKQTVVLGQSANDKPVYLTLTGMSKASSVKFTDEKDYSLQANVTYADGSTGIYNAKFPSGTQEWNRAAVVIPKTKPINKVDISILFQKSATGTVWFDDIRLIEGSLLTKSTYDSNGNYVTKEEDELGYATSTDYDETGKKTSETDAKGEKTTYTYDQADQLTNMTLSNGTSILHSYDKEGNEVSKTIRAGADQTYKFEYDVMGKLVKTTDPLGNVLASEYDANSNLTKTISPNGNEVSLSYDGTDRVKSKSYNGTEKYIFTYDKNGNETSVVNKEQNTTKKRTFDNKNRLTELTDRGGSQTWTYPSDSDKLKTFSWIHGDQKGTNQFTYNKLDQMIEMKDSTSSYSFDYDENGNVQTFITGNGGGTSFSYDERNLVSSLHIGDKNGGDILTESYEYDANGNRTTINSSASGKVQYEYGKLNQLVKETHEDGTVIEYTYDGFGNRKTVTTIKDGSSKTVNASFNIMNQLTKVNDESISYDKNGNRTSDGKFTYTWDAEDNLTAVTKKGEDKPFATYKYDEKGNRIQKTVNGKVTNYFYDGDSLNVLYETDADNNVTKSYTYGDSGQLLSYTENGKKYFYHYNAHGDIIAISDSTGKTVAKYQYDAWGNPTKTEASDEVKDNRYRYAGYQYDEETGLYYLMARYYEPRNGVFLSLDPDPGSDGDSLDQNGYAYGNNNPVMNVDPDGHWVWLVVNAGFAAYDGYKAYKSGKGWKGAAWAAASNFGPGKIFKGASRAYKFTKKAVKITGHTRHGLNQSIGRNGGRGVNLRAKLNAVRSPKKVIKQPNGATKYVGKKATVVLNKRGKVITAYGSSRAKGSKHVFHTHGKGNKSKRRR
What is the function of this protein?
Toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system.  A site-specific general tRNA nuclease, the C-terminus (residues 2201-2334) removes 2 or 4 nucleotides from the 3' end of at least 4 tRNAs (upon expression in E. coli), possibly endonucleolytically.  The nuclease activity is neutralized by expression of the cognate immunity protein WapI from the same strain, but not its homolog from 2 other B. subtilis strains.  The C-terminus cannot be expressed on its own in E. coli, however it can be cloned in the presence of its cognate immunity protein gene.  Cell contact is necessary for growth inhibition (PubMed:23572593).  Unlike the LXG toxin-immunity modules, WapAI mediates competition under shaking culture conditions (PubMed:34280190).
What are the official names of this protein?
tRNA nuclease WapA, Cell wall-associated polypeptide CWBP200, CWBP200, RNase WapA, Toxin WapA, Wall-associated protein.
What is the protein family that this protein belongs to?
Belongs to the RHS/WapA nuclease family.
What are the subcellular locations of this protein?
Secreted, cell wall. Secreted. Note=Probably exported by a SecA-dependent pathway.