Protein: TENS_BEAB2
Sequence: MSPMKQNESESHSVSEPIAIVGSAYRFPGGCNTPSKLWDLLQQPRDILKELDPERLNLRRYYHPDGETHGSTDVSNKAYTLEEDISRFDASFFGISPLEAASMDPQQRTLLEVVYESTETAGIPLDKLRGSLTSVHVGVMTTDWAQVQRRDPETMPQYTATGIASSIISNRISYIFDLKGASETIDTACSSSLVALHNAARALQSGDCEKAIVAGVNLILDPDPFIYESKLHMLSPDARSRMWDAAANGYARGEGAAAVVLKTLGHALRDGDRIEGVIRSTFVNSDGLSSGLTMPSSAAQTALIRQTYRKAGLDPVRDRPQFFECHGTGTRAGDPVEARAISDAFLPSHRTNGGGAATTVDDPLYVGSIKTVVGHLEGCAGLAGLVKVLLSLKHGIIPPNLWFDKLNPEIARYYGPLQIPTKAIPWPELAPGTPLRASVNSFGFGGTNAHAIIERYDASQSYCSQWRRDMTEEKTIARTQNNDDVEIPVPLVLTAKTGGALWRTVDAYAQHLRQHPKLRVANLSQFMHSRRSTHRVRASFSGASREELVENMANFVQAHAADAKSPASQNRIGYSPLLIDPKEVSGILGIFTGQGAQWPAMGRDMMHQSPLFRKTIADCESVLQALPLKDAPAWSLSEELKKDASTSRLGEAEISQPLCTAVQLALVNVLTASGVYFDAVVGHSSGEIAATYASGIINLKAAMQIAYYRGLYAKLARGQSDEAGGMMAAGLSMDDAVKLCRLPEFEGRIQVAASNAPQSVTLSGDKEAIKAAKAKLDADGVFARELKVDTAYHSHHMLPCAEPYLKALLACDIQVSAPTKTPGRKCMWSSSVRGDAELLRRDRNLDSLKGPYWVANMVQTVQFSRAIQSTIWHGGPFDLAVEVGPHPALKGPTEQTLKAVYGSAPLYTGVLSRGANDAVAFSTAIGNIWSHLGPAFVDITGYQSIFSGTCEGHGGSEAPFISDLPLYPWDHDEEYWRESRISRRYRTGKDESHELLGRRMPDDNEREIRWRNLLKVSELPWTQGHRVLGEVLLPGAAYISMAIEAGRRLALDQGREVSLLEVSDVDILRPVVVADNKEGTETLFTVRLLDEYASTGKKSDELMTASFSFYIYNSPASTSIVHTCEGRIAVHLGAKLGSEAAANSTPQLPPREPSVSNLQQLDCEKLYSVFETIGLEYSGAFRRIVSSSRCLGHATATASWPTADLNDCYLVHPAILDVAFQTIFVARAHPDSGQLSSALLPSRIERVRVVPSLAMGSKLQNNENFNAAIDSWALNQTASSLTGNINVYDADSERALIQVEGFEVRAVGEPDASKDRLLFYETVWGRDISIMGLSDPIRDETSDAMVQNLSEAIERVSLFYVRQLMGELSTADRRQANWYHTRMLAAFDHHLAKVHEETHLHLRPEWLADDWTVIQTIDEAYPDAVELQMLHAVGQNVADVIRGKKHLLEVLRVDNLLDRLYTEDKGMHMANLFLANALKEITFKFPRCKILEIGAGTGATTWAALSAIGEAFDTYTYTDLSVGFFENAVERFSAFRHRMVFRALDIEKDPASQSFDLNSYDIIIATNVLHATRNLGVTLGNVRSLLKPGGYLLLNEKTGPDSLRATFNFGGLEGWWLAEEKERQLSPLMSPDGWDAQLQKAQFSGVDHIVHDVQEDQQDKQQNSMIMSQAVDDTFYARLSPLSEMANLLPMNEPLLIIGGQTTATLKMIKEIQKLLPRQWRHKVRLIASVNHLEAEGVPAHSNVICLQELDRGLFTTAMTSKCLDALKTLFINTRNLLWVTNAQHSSSMTPRASMFRGITRVLDGEIPHIRTQVLGIEPRATSSATARNLLEAFLRLRSDDGRHAANVDEDGADGSSQQVLWLHEPEAELLSNGTMMIPRVKARKSLNDTYLASTRAISTTVDARCVSVQAVAGPAKMLLRPVEDFAVEHAISSQSTDSKVHIQVESTLHIPEALDGTCLYLVCGWTRTAETSVPVIALSTSNASIVAVESKAVAMIDEADVKPETLFRVFQHMAMQALDSAVGRHGQGQSTALIYGADEELAKLTSERFAVRESKVYFASTRTSAPGDWLKVQPLLSKFALSQMMPADVEVFIDCLGDTESFDACRTLESCLSTTSTVHRLDACLLSRMSQCSPDTLADAYSHAKTQSNAEFSWNGNVQTFTAAELAGKLSHSLMHSVYMTDWQEKDSILVTVPPLQTRGLFKSDRTYLMVGAAGGLGTSICRWMVRNGARHVVVTSRNPKADPEMLNEARRYGAAVKVVPMDACSKDCVQTVVDMIRDTMPPIAGVCNAAMVLRDKLFLDMNVDHMNNVLGPKMQGTEHLDSIFAQEPLDFFVLLSSSAAILNNTGQSNYHCANLYMDSLVTNRRSRGLAASIIHVGHVCDTGYVARLVDDSKVQMSLGTTRVMSVSETDVHHAFAEAVRGGQPDSRSGSHNIIMGIEPPTKPLDVAKRKPVWISDPRLGHMLPFSTLENQMVASEQAAASAADSLAQQVSEATTDEEAAAAALKGFATKLEGILLLPLGSIGEDSAGRPVTDLGIDSLVAVEIRTWFLKQLRVDVPVMKILGGSTVGQLSALAAKLARQDAKKRAQLEEASGNQPVALPPLNDKETGPSKKGKAQEFPETVQVVGTAAERTEPLVLEASDRGGSSTANFTTSSSVSELDDSLQESTLQSSENNGESTPSKSSNCNSDSGSDNQAPREISSNGFFTQPAATARPNVLREAPMSPAQSRIWFLSKHIAEPDAYNMVFHYRVRGPLSMVRLRHALQTVTNHHECLCMCFYASADNGQPMQGLLASSASQMTIVPGGEEQDLQRELRKLKTRVWSVESGQTLELVVVGPRPGTAAAEEEEFSLLFGYHHIVMDAISFSIFLADLDKAYRMLPLDKASAGSHLDLAAHQRQQEHAGAWKESLEFWQAEFETIPEMLPPLSVALPTLQRGAVGTHRVLRELAHEQGGDAAIKKTCKNLRVSPFNLHIAVLQVVIARLGSIEDVCVGIVDANRSDSRASRMVGCFVNMLPVRSRILPSATLADVARAASSKALAAFAHGQVPLDSILDKVKAPRPAGSTPLFQVALNYRPAAAIASKQSLGGECEMELLADDFKDAENPFEISVLVSEMPGGRIAVEVVCQKSRYTMQATEALLDAYLNVLAGFLSDTAQSVGDCVVHDQSKVEHALDLGKGAQKSFGWPRTLSERVMSICQQHSTKSAIKDGRNELSYAQLASKVNHTASALVNAGCSVGSRIAVLCNPSIDAIVAMLAILHIGGVYVPLDTSLPEARHQSLASNCTPSLIISHAATRERAHKLSAVISAPGHEPARELTLDDLSPDETGYMAPLNAEPNAPAILLYTSGSTGTPKGVLLTQANFGNHIALKTDILGLQRGECVLQQSSLGFDMSLVQVFCALANGGCLVIVPQDVRRDPMELTSLMAQHKVSLTIATPSEYLAWLQYGSDALAQATSWKHLCMGGEPIPQLLKDELRRRLERKDLVVVSNCYGPTETTAAISFQSIALDSQDSHEQLPGESELANYAVGKALPNYSIRIRDPAGGAWLPVNHTGEIVIGGAGVALGYLDMPEETRARFLQTPGEEDGMLLYRTGDKGRLLSDGTLLCFGRITGDNQVKLRGLRIELGEVEAALLQASQGLIHTAVVSRRGDVLVAHCARSHESSRETTGGGGEQQDAATAILRRVSELLPQYSVPAAIALLPSLPTNANGKLDRTAIAALPLSPQDEAAAATSPSNDNNNNNTPSGGGGEKMTVRQGELRLLWERVLPRDATTTTTTNSVRITPESDFFLRGGNSLLLMKLQAAIRESMGVRVSTKALYQASTLSGMARCVAEQRSDDDEAEEDIDWAAEVAVPPSMLAQIEKLQHSSASSSSSSSSSSAGSSSTQRPRKTSGLQILLTGATGFLGGQLLERLVQSPRVSTVHCVAVPVDEQSLLEPFLQQQADGTRRKVRCYIGNLAAPALGLTAADQTALSQTADVIVHAGSMGHCLNTYATLSAPNFASTRHLCALALSRSPPIPLAFASSNRVALLTGSTAPPPGSAAAFPPPPGAQGFTASKWASEAFLEKLTASMSDVSKTKTKTTTTVMPWRVSIHRPCALISDRAPNSDALNAILRYSTSMRCVPSLPEHRAEGYLDFGQVDKVVEEMVGDILGLADERPQEGPAVVYRHHSGGVKVPIHEFREHMESVYGGRFESVQLGQWIIRAVDAGMDPLISAYLETFLEGDASMVFPYMGEQAV
What is the function of this protein?
Hybrid PKS-NRPS synthetase; part of the gene cluster that mediates the biosynthesis of tenellin-type 2-pyridones, iron-chelating compounds involved in iron stress tolerance, competition with the natural competitor fungus Metarhizium robertsii and insect hosts infection (PubMed:17216664, PubMed:18266306, PubMed:20575135, PubMed:34903054).  TenS catalyzes the assembly of the polyketide-amino acid backbone (PubMed:18266306, PubMed:34903054).  Because tenS lacks a designated enoylreductase (ER) domain, the required activity is provided the enoyl reductase tenC (PubMed:18266306, PubMed:34903054).  Upon formation of the polyketide backbone on the thiotemplate, the triketide is transferred to the NRPS module and linked to tyrosine to produce the pyrrolidine-2-dione intermediates, including pretellinin A, 11-hydropretellenin A, 12-hydropretellenin A, 13-hydropretellenin A, 14-hydropretellenin A, 12-oxopretellenin A and prototellinin D (PubMed:18266306, PubMed:34903054).  The pathway begins with the assembly of the polyketide-amino acid backbone by the hybrid PKS-NRPS tenS with the help of the enoyl reductase tenC.  These enzymes catalyze the synthesis of the pyrrolidine-2-dione intermediates pretellinin A, 11-hydropretellenin A, 12-hydropretellenin A, 13-hydropretellenin A, 14-hydropretellenin A, 12-oxopretellenin A and prototellinin D.  The cytochrome P450 monooxygenase tenA then catalyzes an oxidative ring expansion of pretenellin A and 14-hydropretellenin A to form the 2-pyridone core, leading to pretenellin B and pyridovericin, respectively.  The cytochrome P450 monooxygenase tenB is then required for the selective N-hydroxylation of the 2-pyridone nitrogen of yield tellinin and 15-hydroxytellenin (15-HT), respectively.  The UDP-glucosyltransferase GT1 and the methyltransferase MT1, located outside the tenS gene cluster, contribute to the stepwise glycosylation and methylation of 15-HT to obtain the glycoside pyridovericin-N-O-(4-O-methyl-beta-D-glucopyranoside) (PMGP).  Additional related compounds such as 1-O-methyl-15-HT, (8Z)-1-O-methyl-15-HT, and O-methyltenellin A are also produced but the enzymes involved in their biosynthesis have still to be determined (PubMed:34903054).
What are the official names of this protein?
Tenellin synthetase, TENS, Hybrid PKS-NRPS synthetase tenS, Tenellin biosynthesis protein S.
What is the protein family that this protein belongs to?
In the C-terminal section; belongs to the NRP synthetase family.