Protein: HKM11_ASPHA
Sequence: MTVPATTKDPRDSLLDSPLVHFPSLPRDSYLPHPTEHTVVSIDDEEILPSTRSSLHDVLQVAWSLVLADQTSSPEVVFGMAYDGRTTDDGEQAIMPFRLRLRAEDSVQEALAAAAAVTLQMRQWEHMGLRRFSTMSPQNVVLCQFRNLFVIDHKDPTEDCVERSCTSYSKGYALTVLCEIVDQVIHVHAMFDPLVLPPGQLRLILHQFGDILKTCLRGPPGRVKDLQQIGPDGLNYIYEWNRGGERDEGIVCVHQLIEDRFKQAPFAAAVCAWDGALTYGELDRWAKRIAAQLVEAGVKPGSFVGIYMQKSVLAVVAMVAIVKAGAAFIFLPPFLPTVRLQLMCQRTPVELVLSVATLLRSASDLSVPVQVLDYRAKDEEETAATSGGSEIAQPNHPLYAIFTSGSTGEPKGVVVDRASFGPGVREYCRRAQLGPNSRLFQSVSYAFIVSIFEQLISLALGACICVPSEEQLQNDMEGAMCRYQATWGCMTPSVARTLKPERLSCLKTLALTGEPVNQSDMEQWKDHVNLYTLYGQSETGSTLLINSITGSLADSRGLGRPSTGACWIVDPEDPTTLRPLGAEGELLIETTALARGYMNNLEESARTFIEMPKWLKQLRPQGHRSRCLLTGDIVRYYDTDGTIRLLSRKGTGAKIRGQRVELGEIEHHLRPKFPDARHILVDVVCPAKAGTGHSILVAFVHGPWKDTEKTGELATATSEFRQQARRVIAELRQVLPSFMVPSAIVPLADVPTTATGKVHRKSLRERMSALTVAEILAYNQEDRSAYRAPTTEQEALLLSICAELLYLPASSISLDNSFFQVGGDSLNARQLAAKVRSHGFSLLATDIFEASTLASLASRMRQYNQTDSEVSTAPEGDPFEGLKQELLGELPSSLVKENVEDVYPASDMQARAIRTHMLDYFPFEIKGQLDRHQLQHACETLIRRTPVMRSVFINFRGKMLQVTLRSVAIPYKELTIPTGEDPLSWARLHIAEDKKKTAAFDRPTIRFTLCRQSLQHHIFIVRLPHAIYDGSCLEQVAKQISAAYNAQTLPEAPDFAAYARRCARLRTPSAMDFWRNFLAESEVTRLPHASKGDEVAVIYPGECSPRSPPPGITMATAIKAAWAWVLHKRTGKLDVLFGQVGSTRGIDIPGATDIIGLCLNITAVRVQFAGLQTVEDLLRMLQQQHMRALMYETADWTDIVANASSWPEGTELDSVVLHENFGGLPALDLGDAIGEMADPIFSLSTSNPLTLVTWPSTQTLTSFLLTRENVFQKEYAEGLVTEFNQTLVQFLDFPESSLCSISTCG
What is the function of this protein?
Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of hancockiamides, an unusual new family of N-cinnamoylated piperazines (PubMed:33242032).  The NRPS hkm10 and the NmrA-like reductase hkm9 are proposed to convert two molecules of L-Phe to the intermediary piperazine called xenocockiamide A (Probable).  Xenocockiamide A is then converted to hancockiamide D via a series of hydroxylations and O-methylations (Probable).  The tyrosinase hkm6 may catalyze an aromatic hydroxylation, then the 2-oxoglutarate-dependent Fe(II) dioxygenase hkm4 and the FAD-dependent phenol hydroxylase hkm7 may catalyze consecutive hydroxylations to install 2 more hydroxy groups, and the methyltransferase hkm8 probably catalyzes two methylations using 2 molecules of S-adenosyl-L-methionine (SAM) (Probable).  The NRPS hkm11 activates and transfers trans-cinnamate supplied by the PAL hkm12 to hancockiamide D and produces hancockiamide A (PubMed:33242032).  NRPS Hkm11 has the flexibility to tolerate the bulky hancockiamide G as a substrate and the absence of the acetyl-transferase hkm3 opens up the opportunity for hkm11 to introduce a second N-cinnamoyl moiety (PubMed:33242032).  The cytochrome P450 monooxygenase hkm5 catalyzes the methylenedioxy bridge formation, converting hancockiamide A into hancockiamide G (PubMed:33242032).  Hkm5 can also convert hancockiamide B into hancockiamide C, and hancockiamide D into hancockiamide H (PubMed:33242032).  The N-acetyltransferase hkm3 finally transfers an acetyl group to 1-N of piperazine, converting hancockiamide A into hancockiamide B and hancockiamide G into hancockiamide C (PubMed:33242032).
What are the official names of this protein?
Nonribosomal peptide synthetase hkm11, Hancockiamides biosynthesis cluster protein 11.
What is the protein family that this protein belongs to?
Belongs to the NRP synthetase family.