Protein: PHQL_PENFE
Sequence: MEPHHDGHILKVLPMLASNENFSRLTTAFVAGIAAHIIIFRRGEWDIAAARIPVGLFILQSCLFSYYLFVPGPPTSIYTALWLVGQITLGFIAGTTVSILSYRAFFHRLNSFPGPFPARLSMWYVTSLYAQNPDAFNTVRGLHQQYGDFVRTGPTELSVNHPDALQAVHSGRSECTKGPWYSMLHPFISLFAIRDKAEHSRRRKPWELAFRPNAVLEYLPALEKGTNELLEQVERRKGKSMDMTYWINLFTFDLTGRVAFSQEYECVKHNKRHPIMEINDSSNLVTGVVSHVVWLISFIKATPGLNANMKALIGFSEEQVQNRQKMQTSGQRDVFSWLWEDFKEQGMETPQSKLDLVADASLVIFAGSGTVAVTIIGCLYFLTSTSPDYLTQIRQELDTLDEINSHTLSKVQTLNAVINETLRLHYPALSGFQRQTPPGGLHIAGRYIPGNTNIKIPFYTLFLDERNFAEPEKFIPERWTTRKELVKNPEAFAPFLLGPYNCLGKSLALMQVRHVLVELIRRYEIVLAPGADPEKYWRERTDGFVMGLAPLDLAFTEREMAGF
What is the function of this protein?
Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of paraherquamide, a fungal indole alkaloid that belongs to a family of natural products containing a characteristic bicyclo[2. 2. 2]diazaoctane core (PubMed:23213353).  The first steps in the biosynthesis of paraherquamide is the production of the beta-methyl-proline precursor from L-isoleucine (Probable).  They require oxidation of a terminally hydroxylated L-isoleucine to the corresponding aldehyde by enzymes which have still to be identified (Probable).  Spontaneous cyclization and dehydration would yield the 4-methyl pyrolline-5-carboxylic acid, which is then reduced by the pyrroline-5-carboxylate reductase phqD leading to the beta-methyl-proline precursor (Probable).  The next step of paraherquamide biosynthesis involves coupling of beta-methyl-proline and L-tryptophan by the bimodular NRPS phqB, to produce a monooxopiperazine intermediate (Probable).  The reductase (R) domain of phqB utilizes NADPH for hydride transfer to reduce the thioester bond of the T domain-tethered linear dipeptide to a hemithioaminal intermediate, which spontaneously cleaves the C-S bond to release the aldehyde product (PubMed:31548667).  This compound undergoes spontaneous cyclization and dehydration to give a dienamine which is reverse prenylated at C-2 by the reverse prenyltransferase phqJ (Probable).  The other prenyltransferase present in the cluster, phqI may be a redundant gene in the pathway (Probable).  During biosynthetic assembly, the key step to produce the polycyclic core is catalyzed by the bifunctional reductase and intramolecular [4+2] Diels-Alderase, phqE, resulting in formation of the [2. 2. 2] diazaoctane intermediate preparaherquamide (PubMed:31548667).  Following formation of preparaherquamide, an indole 2,3-epoxidation-initiated pinacol-like rearrangement is catalyzed by the phqK FAD-dependent monooxygenase (Probable).  The prenyltransferase phqA, the cytochrome P450 monooxygenase phqL, and the FAD-linked oxidoreductase phqH (or the cytochrome P450 monooxygenase phqM), are proposed to be involved in the formation of the pyran ring (Probable).  The FAD-dependent monooxygenase phqK is likely responsible for generation of the spiro-oxindole, and the N-methylation is likely mediated by the phqN methyltransferase leading to the isolable natural product paraherquamide F (Probable).  However, the order of these biosynthetic steps has still to be determined (Probable).  In late-stage paraherquamide biosynthesis, the third P450 monooxygenase, phqO, is probably responsible for the C-14 hydroxylation, transforming paraherquamide F to paraherquamide G, and paraherquamide E to the final product paraherquamide A (Probable).  The expansion from the 6-membered ring pyran (in paraherquamides F and G) to the 7-membered dioxepin ring (in paraherquamides A and E) represents a poorly understood but intriguing process that probably involves the 2-oxoglutarate-dependent dioxygenase phqC (Probable).  Finally, the remaining members of the paraherquamide cluster, including phqI as well as phqM (or phqH), do not have a clearly prescribed role and appear to be redundant (Probable).
What are the official names of this protein?
Cytochrome P450 monooxygenase phqL, Paraherquamide biosynthesis cluster protein L.
What is the protein family that this protein belongs to?
Belongs to the cytochrome P450 family.
What are the subcellular locations of this protein?
Membrane; Multi-pass membrane protein.