Protein: XENE_XENSI
Sequence: MSGQDPVKESGQREPIAVVGSGFRFPGSSNNPSKLWDLLVKPRDLLTKIPENRFNSDAFYHPKPFHHGTSDVRESYFLEEDHRQFDAAFFNIKPVEVHAIDPQQRILMEAVYESLEAAGLSMESLAGSRTGVYVGLMCADYVDLLNNDVNSLPTYTPTGTARSIMSNRISYFFDWHGPSMTIDTACSSSLVAVHQAVQLLRSGDSDVAVAAGANLMLGPLPYIAESKLQMLSSNSRSRMWDIDASGYARGEGVAAVVLKRLSSAIADGDQIECIIRESGINQDGRTKGITMPSSVAQADLISRTYAKAGLNPRDPTERCQYFEAHGTGTAAGDPKEAEAISKAFFHPGEDISGKTDPLYVGSIKTVIGHTEGTAGLAGLLKASLAVQHGIVPPNLLFNQLSPAVEPFYTNLEVLTSPRPWPKLAEGTPRRASINSFGFGGTNAHCIIENYIPSPAHTDRAITTRQFTPFNFSAASEKSLRGILTDYSNYLRLNPEVSLQDLSYTLYARRSEHAVRVHISAGSTTDLYTRIDDLLQVPSSGGNAQSIGTRSKILSRPVRALGVFTGQGAQWPSMGRELVLNSPYAKEVVQKLDLVLQSLPEPERPDWSLMYELTCDASQSRLNTAVIAQPLCTVVQIILFDLLSSAGVKLQAVVGHSSGEIAAAYAAGYLTREDALKIAYYRGYFTNLTPSDRPGAMMAIGTSAEDAEELCSLPMFQGRLAVAAVNSSSSVTISGDRDAIEQAKEVLEDEKKFARLLKVDKAYHSSHMVPCAEGYLEALKNSEIHPLSGTDDCVWHSSTHKNKYSHGDGALAGQYWADNMIQPVLFSHAVEAAASAGDAFDIAIEVGPHPALKGPALQTLQEVQKDTIPYTGLLNRGKDDIEALSDALGYLWTQFTPSLIDFRGFDLLASGGEQRSLIRNLPTYHWDHDKIFWHKSRAVKAFLGQKNIPNPLLGSRTTDVMEQEIRWRNLLRLSELPWVRGHQLQGQVIYPATAYISTAVEAARFLVPKGDNIALIEVEDFSLGKPLVFAEDAAGIETVFTLSDIAKENDTTYSASFIYHASTNAETEQLSTHAIGRVIVITGETSSHWLPSRQKDLPNLVDIPEDRFYASLEPLGYAYSGYFKTMSSIKRRLNFSSTKIRVPPQDDEPEKMLLHPALLDTALQGIFLAYCWPGDGSLEQLHVPTGIKNFRVNVGLCQQVLTPETDVSSCTQLTGNPLATKHLNGDVEIYADDGAGLVQMEGLRVVAFAEQTEDADRAIFSEHVWDVLAPNCERAMGGKRATLQDYEFAYGMERVVVYYMKQLVTLFPESLRKIMNLEWHFECMFAFFTDVLTTLEAGERRTARREWLQDTAADVEGIKARYAHTVDMQLTCAVGDNLPAVLRGESTILQHLTKDNLLNRFYEVGLGLKEVSGYLGKIVEQVVHRHPRMKILEIGKSGTGGATKVIMRGIGRSFSSYTYTDISPNFFESAQEVFSAVADKMIFKTLDVEKDITEQSFEEHSYDLVVASLVLHATTNLKRTLTNARRLLKPGGYLIFQEICDNDIARVGFLICAVPGWWLGQDDGRKLSPCVSTSEWHNLLLETGFSGADSPMPEYDAAPYPLAVIVSQAVDDRIALLREPLSLVQNDASVGEPWDLVLVGGQTSKTAMIIEQISGLITSSGVTHSVFKTIDEVDGTRISPTTAILCLADLDEPVFKGLSSTTLEGLQRLFETQRTVLWITQGCRSEDPWMNMSVGLGRTLVLENPDLALQFLDLEPGVEPNPRQLLEVLLRLRQSDIWEKEGKFDDVLWTNEHELAYDKGDLTLSRVHLSGALNDRYNAAKRTVLEAKNPQETPLNLSLGPSLKQFLVLDDVLVAKTLSSWELKDDSETLIKVTHSLLMPALAAPTPLYLILGTINKTKKSVLSIADNNGSYALVASNKVLAIDVPAGQESQLLSLFNTRLQVDSMLSLCESDSTLLIHEPSPDLASAIAACGSSGKINVVFTTSTSSGDSTWTRIDAYSTQRAIRSLLPENVSVFIDCSAGSQSRRTASLIASCLLPSCFQTTISGVQSLQRIRGLSPTDLCQKLNDALAWASKELAAPSNPGTLPSIKLETLIDDSAVASTTQAVVDWSTATSVPVQVSTVDNHVTFKGNNTYVLFGLTSDLAQSICDWMVSRGARNIVLTSRNPKIDSNWIELLKGAGVRLEAFANDITNKDALSSLVHHIRKNFPPIAGVAHGAMVLDDVSFFEMPYEKMTKVLGPKVQGAILLDEIFQDTSLDFFVFFSSVTAIAGNRGQSAYTSANMFMTSLASQRRDKGLAASILHLGAVMGVGYINRGFSDAFFTTLRRAGFMMMSERGLHLLFGEAVLASNPHSGRNPEVITALELSRLGDKPPLWTKFPRFQHCLQADDGANKRAKKKTAAVSTKLKLAEATTAEEILEIVQDAFYLKLQVALQIPDETDKSQVLASGTDDLGIDSLVAVEIRSWFLKELETEIPVFKVLSGGSVTQLVEYAIGSMPAELTPNRADSAKASEPEPEAPATLMPPPDSVSSSPSSLPKTSASGSSQQMSEGSSKTSEQGDAQDKKEESPSESVNDISELTYEKVLPVSPGQSRFWFLKHLLEDQTTANNTIWVSIQGTIRLNDLEMAIRKVAARHEALRTSFFMDENQKPIQAISETSRLYLEKKTLSSGSQAEREFEGLKKHVYDIEHGECMRLVYLEVTDTESYLLIGSHHIIMDGISLEVFLKDIEKAYNGQSLSNQVYQYSDYSEKLRQELEQGTMQEEINYWKSEFADVPSPLPLLPFAAEKQRKSLAAYSHTSVSRLVDPRVARQISNTCHKLKANVFHFYLGVFEVLLFKLFGNNDVCIGMADANRWNEKVSQSIGMYLNLLPLRFHLDGRQSFEAMLKDTRRKAYLAMSNSRLPFDVLLDNVICERSSAFSPLFQAFINYRQGVNEKRVLGNATGATKELSLPRAGYDISLDIIENPGNDTRVTVMLQKALYSDNESSRVLDLYFKLLNDLSSSSKKMLEEVSLFTEQEISNSIQLGQGPVLPSQWPETLVHRIDAMIAVHTDKVALKEITGKSWTYLQLEEEINRVSSVLIQANVTSGSTVAIYQEASPNFVFSLLAVLRIGAIYVPLDCNLPEGRLRLVLAECKPSALLADGKTLSQIGSLGLSPSVTILDVSRLPAASASISPVFTKAANPAAILFTSGSTGVPKGVVLSHGSLRNHVEALVHTHGFGSETVLQQSSVGFDMSMNQVFMALANGGSLVIVPESLRKDSSAIAKILLEQNITYTSATPSEYFAWLRHGSDDLLRNKSWKYATAGGEKFTPKLLQAFQKLKSAFSHSFHAFNAYGPTECSMSSNELEVNLDGHSAQFITAGRALPNYAVYIVDENATPQTIEIPGEICIAGAGVALEYLNNPVETAKKFLKDPFASVSAIERGWNRMYRTGDKGVLRPDGTLEILGRIEGDTQIKLRGLRIEMQDIEQSILKAGEGRVKEAIVTPRGDPTILVAHAVVSPIVSIDNEREYLRGLAASLPLPQYMRPAAIIPIATMPLNASGKIDRRALQNLDIPSTLQQKTRSKRKLTDTESKLAQIWVEVLPQQLQEVYAIDETSDFFQVGGNSMLLIELRELVKKRFQVHLPLLRFFEHSTLGAMAAAIQDTSPGEKLEINWDVETEVPSAYSELNTQEPAQSHSSHKTIVLTGATGFLGKYLLNLLTEAPDVDKIHCIAIRNREKLANFTNSAKVVIHDGDLASPRCGLSEADATSVFGSANVIIHNGADVSFLKTYSSLRASNVLSTKELVKLALPHHIPIHYISTATVGKLNKSDSLAPESLAQYPPGPSFVDGYAASKWASEVFLEKTTRQFGLPTFIHRPSSITGDGAGENDIVPSVLKYSAMIKALPDSSKWTGYIDLITVEKAAAGIADSVLQGRLVNVTATEAEYLHHAGEKVIPAQSIKTILTADDGPQWESVSMKNWVEKAIQNGMNPLVGEFLLSIDKGQGMQIGQKLLSKTDGSKNEF
What is the function of this protein?
Hybrid PKS-NRPS synthetase; part of the gene cluster that mediates the biosynthesis of xenoacremones such as xenoacremone A, a compound that shows inhibitory activity toward the PI3K/AKT signaling pathway and which has the ability to induce apoptosis of A549 lung cancer cells (PubMed:34900544).  Within the pathway, cooperation of the hybrid PKS-NRPS xenE and the trans-acting enoyl reductase xenG is responsible for the formation of the reduced tyrosine-nonaketide derivative (PubMed:34900544).  The PKS module of xenE acted in combination with the trans-acting enoyl reductase xenG to produce a double-methylated nonaketide attached to the ACP domain (PubMed:34900544).  In parallel, the adenylation (A) domain of the NRPS module activated L-tyrosine, which was then transferred to the ACP domain (PubMed:34900544).  The condensation (C) domain subsequently linked this group to the polyketide chain, forming an enzyme-bound amide (PubMed:34900544).  Reductive release by the C-terminal R domain afforded the aldehyde derivative (PubMed:34900544).  The alpha/beta hydrolase xenA then accelerates intramolecular nucleophilic attack to give a pyrrolidone derivative (PubMed:34900544).  Subsequently, three enzymes, xenF, xenD, and xenC, coordinately participate in the conversion to xenoacremone B (PubMed:34900544).  XenF catalyzes sigmatropic rearrangement to form an A-ring, which leads to an unusual intermediate with a hexane ring, which is required for the formation of the tricarbocyclic product (PubMed:34900544).  Epoxidation catalyzed by xenD and the formation of the paracyclophane ether catalyzed by xenC initiate a spontaneous intramolecular Diels-Alder (IMDA) reaction to yield xenoacremone B (PubMed:34900544).  Spontaneous hydration of xenoacremone B leads to the formation of xenoacremone A, which undergoes subsequent methylation to afford xenoacremone C (PubMed:34900544).
What are the official names of this protein?
Hybrid PKS-NRPS synthetase xenE, Xenoacremones biosynthesis cluster protein E.
What is the protein family that this protein belongs to?
In the C-terminal section; belongs to the NRP synthetase family.