Middle sporulation element gene transcriptions
Ndt80 is a meiosis-specific transcription factor required for successful completion of meiosis and spore formation. The DNA-binding domain of Ndt80 has been isolated, and the structure reveals that this protein is a member of the Ig-fold family of transcription factors. Ndt80 also competes with the repressor SUM1 for binding to promoters containing MSEs.
Direct "binding of Ndt80 to the  MSE is necessary for activating transcription of the middle sporulation genes."
"The midsporulation gene SPR3  is expressed only in sporulating cells (20), during both divisions of meiosis. It is therefore regulated in a developmentally specific manner and provides an example of a sporulation gene suited to analysis of midsporulation control. The 5'-flanking region contains several potential regulatory motifs, including an ABFI element which was shown to be essential to the regulation of SPR3 (33)."
"The SPR3 gene contains four potential TATA boxes, with the most proximal one adjacent to the transcription start sites identified previously (33). The MSE is located 43 to 102 bp upstream of these TATA boxes, locating the sporulation-specific element very close to the site for assembly of the general transcription machinery."

# Human genes

# Interactions

# MSE consensus sequences
Of the midsporulation element (MSE), "a minimal element, CRCAAA(A/T), is sufficient for sporulation specificity."
"These genomic sequences were analysed for the presence of  middle sporulation elements (MSE) motif (5'-ACACAAA-3') using the NCBI BLAST tool."
Middle sporulation elements (MSE) motif has the consensus sequence 5'-ACACAAA-3'.

# MSE (Branco) samplings
Copying apparent core consensus sequences for the MSEs of ACACAAA and putting it in "F" finds none located between ZSCAN22 and none between ZNF497 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence ACACAAA (starting with SuccessablesMSEB.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for ACACAAA, 0.
- positive strand, negative direction, looking for ACACAAA, 0.
- positive strand, positive direction, looking for ACACAAA, 0.
- negative strand, positive direction, looking for ACACAAA, 0.
- complement, negative strand, negative direction, looking for TGTGTTT, 0.
- complement, positive strand, negative direction, looking for TGTGTTT, 0.
- complement, positive strand, positive direction, looking for TGTGTTT, 0.
- complement, negative strand, positive direction, looking for TGTGTTT, 0.
- inverse complement, negative strand, negative direction, looking for TTTGTGT, 2, TTTGTGT at 3513, TTTGTGT at 1541.
- inverse complement, positive strand, negative direction, looking for TTTGTGT, 0.
- inverse complement, positive strand, positive direction, looking for TTTGTGT, 1, TTTGTGT at 2835.
- inverse complement, negative strand, positive direction, looking for TTTGTGT, 0.
- inverse negative strand, negative direction, looking for AAACACA, 0.
- inverse positive strand, negative direction, looking for AAACACA, 2, AAACACA at 3513, AAACACA at 1541.
- inverse positive strand, positive direction, looking for AAACACA, 0.
- inverse negative strand, positive direction, looking for AAACACA, 1, AAACACA at 2835.

## MSE (Branco) (4560-2846) UTR gene transcriptions
- Negative strand, negative direction: TTTGTGT at 3513

## MSE (Branco) negative direction (2596-1) distal promoters
- Negative strand, negative direction: TTTGTGT at 1541.

## MSE (Branco) positive direction (4050-1) distal promoters
- Positive strand, positive direction: TTTGTGT at 2835.

# MSE (Branco) random dataset samplings
- MSEBr0: 0.
- MSEBr1: 0.
- MSEBr2: 0.
- MSEBr3: 0.
- MSEBr4: 2, ACACAAA at 1970, ACACAAA at 1775.
- MSEBr5: 0.
- MSEBr6: 0.
- MSEBr7: 0.
- MSEBr8: 0.
- MSEBr9: 0.
- MSEBr0ci: 0.
- MSEBr1ci: 0.
- MSEBr2ci: 0.
- MSEBr3ci: 0.
- MSEBr4ci: 0.
- MSEBr5ci: 1, TTTGTGT at 323.
- MSEBr6ci: 1, TTTGTGT at 4365.
- MSEBr7ci: 0.
- MSEBr8ci: 0.
- MSEBr9ci: 0.

## MSEBr arbitrary (evens) (4560-2846) UTRs
- MSEBr6ci: TTTGTGT at 4365.

## MSEBr alternate positive direction (evens) (4445-4265) core promoters
- MSEBr6ci: TTTGTGT at 4365.

## MSEBr arbitrary negative direction (evens) (2596-1) distal promoters
- MSEBr4: ACACAAA at 1970, ACACAAA at 1775.

## MSEBr alternate negative direction (odds) (2596-1) distal promoters
- MSEBr5ci: TTTGTGT at 323.

## MSEBr arbitrary positive direction (odds) (4050-1) distal promoters
- MSEBr5ci: TTTGTGT at 323.

## MSEBr alternate positive direction (evens) (4050-1) distal promoters
- MSEBr4: ACACAAA at 1970, ACACAAA at 1775.

# MSE (Branco) analysis and results
The Pax-4 homeodomain  was shown to preferentially dimerize on DNA sequences consisting of an inverted TAAT motif, separated by 4-nucleotide spacing."
Comparison:
The occurrences of real MSE (Branco)s are greater than the randoms. This suggests that the real MSE (Branco)s are likely active or activable.

# MSE (Ozsarac) samplings
Copying a responsive elements consensus sequence C(A/G)CAAA(A/T) and putting the sequence in "F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence C(A/G)CAAA(A/T) (starting with SuccessablesMSEX.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for C(A/G)CAAA(A/T), 0.
- positive strand, negative direction, looking for C(A/G)CAAA(A/T), 2, CACAAAA at 3767, CGCAAAA at 164.
- positive strand, positive direction, looking for C(A/G)CAAA(A/T), 0.
- negative strand, positive direction, looking for C(A/G)CAAA(A/T), 0.
- complement, negative strand, negative direction, looking for G(C/T)GTTT(A/T), 2, GTGTTTT at 3767, GCGGGGG at 164.
- complement, positive strand, negative direction, looking for G(C/T)GTTT(A/T), 0.
- complement, positive strand, positive direction, looking for G(C/T)GTTT(A/T), 0.
- complement, negative strand, positive direction, looking for G(C/T)GTTT(A/T), 0.
- inverse complement, negative strand, negative direction, looking for (A/T)TTTG(C/T)G, 1, TTTTGTG at 3512.
- inverse complement, positive strand, negative direction, looking for (A/T)TTTG(C/T)G, 0.
- inverse complement, positive strand, positive direction, looking for (A/T)TTTG(C/T)G, 0.
- inverse complement, negative strand, positive direction, looking for (A/T)TTTG(C/T)G, 0.
- inverse negative strand, negative direction, looking for (A/T)AAAC(A/G)C, 0.
- inverse positive strand, negative direction, looking for (A/T)AAAC(A/G)C, 1, AAAACAC at 3512.
- inverse positive strand, positive direction, looking for (A/T)AAAC(A/G)C, 0.
- inverse negative strand, positive direction, looking for (A/T)AAAC(A/G)C, 0.

## MSE (Ozsarac) UTR gene transcriptions
Negative strand, negative direction: TTTTGTG at 3512
Positive strand, negative direction: CACAAAA at 3767

## MSE (Ozsarac) distal promoters
Positive strand, negative direction: CGCAAAA at 164

# MSE (Ozsarac) random dataset samplings
- MSEXr0: 2, CGCAAAT at 2807, CACAAAT at 1788.
- MSEXr1: 3, CGCAAAA at 4408, CACAAAA at 3175, CGCAAAA at 1775.
- MSEXr2: 1, CACAAAA at 4322.
- MSEXr3: 1, CGCAAAT at 3362.
- MSEXr4: 2, CACAAAA at 1971, CACAAAA at 1776.
- MSEXr5: 1, CGCAAAA at 752.
- MSEXr6: 4, CACAAAT at 3077, CACAAAA at 1779, CACAAAA at 970, CGCAAAT at 362.
- MSEXr7: 2, CGCAAAT at 2393, CGCAAAT at 660.
- MSEXr8: 2, CACAAAT at 3714, CGCAAAA at 2111.
- MSEXr9: 1, CACAAAT at 4053.
- MSEXr0ci: 0.
- MSEXr1ci: 1, TTTTGTG at 282.
- MSEXr2ci: 2, TTTTGTG at 4487, TTTTGCG at 37.
- MSEXr3ci: 1, ATTTGCG at 3972.
- MSEXr4ci: 2, TTTTGCG at 2429, TTTTGCG at 1426.
- MSEXr5ci: 3, ATTTGTG at 3880, TTTTGTG at 322, TTTTGCG at 276.
- MSEXr6ci: 1, TTTTGTG at 2653.
- MSEXr7ci: 2, ATTTGTG at 4226, TTTTGTG at 493.
- MSEXr8ci: 1, ATTTGCG at 2528.
- MSEXr9ci: 1, TTTTGTG at 470.

## MSEXr arbitrary (evens) (4560-2846) UTRs
- MSEXr2: CACAAAA at 4322.
- MSEXr6: CACAAAT at 3077.
- MSEXr8: CACAAAT at 3714.
- MSEXr2ci: TTTTGTG at 4487.

## MSEXr alternate (odds) (4560-2846) UTRs
- MSEXr1: CGCAAAA at 4408, CACAAAA at 3175.
- MSEXr3: CGCAAAT at 3362.
- MSEXr9: CACAAAT at 4053.
- MSEXr5ci: ATTTGTG at 3880.
- MSEXr7ci: ATTTGTG at 4226.

## MSEXr arbitrary positive direction (odds) (4445-4265) core promoters
- MSEXr1: CGCAAAA at 4408.

## MSEXr alternate positive direction (evens) (4445-4265) core promoters
- MSEXr2: CACAAAA at 4322.

## MSEXr arbitrary negative direction (evens) (2811-2596) proximal promoters
- MSEXr0: CGCAAAT at 2807.
- MSEXr6ci: TTTTGTG at 2653.

## MSEXr arbitrary positive direction (odds) (4265-4050) proximal promoters
- MSEXr9: CACAAAT at 4053.
- MSEXr7ci: ATTTGTG at 4226.

## MSEXr arbitrary negative direction (evens) (2596-1) distal promoters
- MSEXr0: CACAAAT at 1788.
- MSEXr4: CACAAAA at 1971, CACAAAA at 1776.
- MSEXr6: CACAAAA at 1779, CACAAAA at 970, CGCAAAT at 362.
- MSEXr8: CGCAAAA at 2111.
- MSEXr2ci: TTTTGCG at 37.
- MSEXr4ci: TTTTGCG at 2429, TTTTGCG at 1426.
- MSEXr8ci: ATTTGCG at 2528.

## MSEXr alternate negative direction (odds) (2596-1) distal promoters
- MSEXr1: CGCAAAA at 1775.
- MSEXr5: CGCAAAA at 752.
- MSEXr7: CGCAAAT at 2393, CGCAAAT at 660.
- MSEXr1ci: TTTTGTG at 282.
- MSEXr5ci: TTTTGTG at 322, TTTTGCG at 276.
- MSEXr9ci: TTTTGTG at 470.
- MSEXr7ci: TTTTGTG at 493.

## MSEXr arbitrary positive direction (odds) (4050-1) distal promoters
- MSEXr1: CACAAAA at 3175, CGCAAAA at 1775.
- MSEXr3: CGCAAAT at 3362.
- MSEXr5: CGCAAAA at 752.
- MSEXr7: CGCAAAT at 2393, CGCAAAT at 660.
- MSEXr1ci: TTTTGTG at 282.
- MSEXr5ci: ATTTGTG at 3880, TTTTGTG at 322, TTTTGCG at 276.
- MSEXr9ci: TTTTGTG at 470.
- MSEXr7ci: TTTTGTG at 493.

## MSEXr alternate positive direction (evens) (4050-1) distal promoters
- MSEXr0: CGCAAAT at 2807, CACAAAT at 1788.
- MSEXr4: CACAAAA at 1971, CACAAAA at 1776.
- MSEXr6: CACAAAT at 3077, CACAAAA at 1779, CACAAAA at 970, CGCAAAT at 362.
- MSEXr8: CACAAAT at 3714, CGCAAAA at 2111.
- MSEXr2ci: TTTTGCG at 37.
- MSEXr4ci: TTTTGCG at 2429, TTTTGCG at 1426.
- MSEXr8ci: ATTTGCG at 2528.

# MSE (Ozsarac) analysis and results
Of the midsporulation element (MSE), "a minimal element, CRCAAA(A/T) , is sufficient for sporulation specificity."
Comparison:
The occurrences of real MSE (Ozsarac) UTRs are greater than the randoms, but the negative distals are less than randoms. This suggests that the real MSE (Ozsarac)s are likely active or activable.

# HAP2 consensus sequences
"The HAP2 motif  binds a heterotrimeric transcription factor, HAP2/3/4, involved in the derepression of respiratory genes in cells growing on nonfermentable carbon sources (34)."

# HAP2 samplings
Copying a responsive elements consensus sequence CCAATCA and putting the sequence in "F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence CCAATCA (starting with SuccessablesHAP.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for CCAATCA, 0.
- positive strand, negative direction, looking for CCAATCA, 0.
- positive strand, positive direction, looking for CCAATCA, 0.
- negative strand, positive direction, looking for CCAATCA, 0.
- complement, negative strand, negative direction, looking for GGTTAGT, 0.
- complement, positive strand, negative direction, looking for GGTTAGT, 0.
- complement, positive strand, positive direction, looking for GGTTAGT, 0.
- complement, negative strand, positive direction, looking for GGTTAGT, 0.
- inverse complement, negative strand, negative direction, looking for TGATTGG, 0.
- inverse complement, positive strand, negative direction, looking for TGATTGG, 0.
- inverse complement, positive strand, positive direction, looking for TGATTGG, 0.
- inverse complement, negative strand, positive direction, looking for TGATTGG, 0.
- inverse negative strand, negative direction, looking for ACTAACC, 0.
- inverse positive strand, negative direction, looking for ACTAACC, 0.
- inverse positive strand, positive direction, looking for ACTAACC, 0.
- inverse negative strand, positive direction, looking for ACTAACC, 0.

# Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.