Zinc responsive element gene transcriptions
"A robust set of genes that responded consistently to Zn limitation was identified, and the set enabled the definition of the Zn-specific Zap1p regulon, comprised of 26 genes and characterized by a broader zinc-responsive element consensus (MHHAACCBYNMRGGT) than so far described."
"In order to better define the Zap1p regulon, Lyons and coworkers analyzed the genome-wide transcriptional response of a S. cerevisiae zap1 mutant strain and a control strain to zinc abundance or depletion (50). A combinatorial analysis identified a subset of 46 zinc-responsive genes whose expression was reduced in the zap1 mutant and that possessed a zinc-responsive element (ZRE) (5-ACCYYNAAGGT-3). Among the members of this updated defined Zap1p regulon were the well-characterized plasma membrane, vacuole, and endoplasmic reticulum zinc transporters."

# Consensus sequences
A zinc-responsive element consensus (MHHAACCBYNMRGGT) is (A/C)(A/C/T)(A/C/T)AACC(C/G/T)(C/T)N(A/C)(A/G)GGT.

# ZRE samplings
Copying a responsive elements consensus sequence (A/C)(A/C/T)(A/C/T)AACC(C/G/T)(C/T)N(A/C)(A/G)GGT and putting the sequence in "F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs. The addition of degenerate nucleotides increases the possible occurrences to 216 from 1.
For the Basic programs testing consensus sequence (A/C)(A/C/T)(A/C/T)AACC(C/G/T)(C/T)N(A/C)(A/G)GGT (starting with SuccessablesZRE.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for (A/C)(A/C/T)(A/C/T)AACC(C/G/T)(C/T)N(A/C)(A/G)GGT, 0.
- positive strand, negative direction, looking for (A/C)(A/C/T)(A/C/T)AACC(C/G/T)(C/T)N(A/C)(A/G)GGT, 0.
- positive strand, positive direction, looking for (A/C)(A/C/T)(A/C/T)AACC(C/G/T)(C/T)N(A/C)(A/G)GGT, 0.
- negative strand, positive direction, looking for (A/C)(A/C/T)(A/C/T)AACC(C/G/T)(C/T)N(A/C)(A/G)GGT, 0.
- complement, negative strand, negative direction, looking for (G/T)(A/G/T)(A/G/T)TTGG(C/G/A)(A/G)N(G/T)(C/T)CCA, 0.
- complement, positive strand, negative direction, looking for (G/T)(A/G/T)(A/G/T)TTGG(C/G/A)(A/G)N(G/T)(C/T)CCA, 0.
- complement, positive strand, positive direction, looking for (G/T)(A/G/T)(A/G/T)TTGG(C/G/A)(A/G)N(G/T)(C/T)CCA, 0.
- complement, negative strand, positive direction, looking for (G/T)(A/G/T)(A/G/T)TTGG(C/G/A)(A/G)N(G/T)(C/T)CCA, 0.
- inverse complement, negative strand, negative direction, looking for ACC(C/T)(G/T)N(A/G)(A/C/G)GGTT(A/G/T)(A/G/T)(G/T), 0.
- inverse complement, positive strand, negative direction, looking for ACC(C/T)(G/T)N(A/G)(A/C/G)GGTT(A/G/T)(A/G/T)(G/T), 0.
- inverse complement, positive strand, positive direction, looking for ACC(C/T)(G/T)N(A/G)(A/C/G)GGTT(A/G/T)(A/G/T)(G/T), 0.
- inverse complement, negative strand, positive direction, looking for ACC(C/T)(G/T)N(A/G)(A/C/G)GGTT(A/G/T)(A/G/T)(G/T), 0.
- inverse negative strand, negative direction, looking for TGG(A/G)(A/C)N(C/T)(C/G/T)CCAA(A/C/T)(A/C/T)(A/C), 0.
- inverse positive strand, negative direction, looking for TGG(A/G)(A/C)N(C/T)(C/G/T)CCAA(A/C/T)(A/C/T)(A/C), 0.
- inverse positive strand, positive direction, looking for TGG(A/G)(A/C)N(C/T)(C/G/T)CCAA(A/C/T)(A/C/T)(A/C), 0.
- inverse negative strand, positive direction, looking for TGG(A/G)(A/C)N(C/T)(C/G/T)CCAA(A/C/T)(A/C/T)(A/C), 0.

# Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.