Ethylene responsive element gene transcriptions
"In vitro DNA binding assays associate  ERF family members with GCC-box motifs (5-AGCCGCC-3) (Ohme-Takagi and Shinshi, 1995; Hao et al., 1998), which have been traced out in many genes induced by ethylene, pathogenesis, wounding, or jasmonate (Brown et al., 2003; Zarei et al., 2011)."

# Human genes

# Consensus sequences
Ethylene responsive elements (ATTTCAAA).

# Hypotheses
- A1BG has no ethylene responsive elements in either promoter.
- A1BG is not transcribed by an ethylene responsive element.
- An ethylene responsive element does not participate in the transcription of A1BG.

# ERE samplings
Copying an apparent consensus sequence of ATTTCAAA and putting it in "F" finds none located between ZSCAN22 and A1BG and one between ZNF497 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence ATTTCAAA (starting with SuccessablesEth.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for 5'-ATTTCAAA-3', 0.
- negative strand, positive direction, looking for 5'-ATTTCAAA-3', 1, ATTTCAAA at 2648.
- positive strand, negative direction, looking for 5'-ATTTCAAA-3', 1, ATTTCAAA at 1383.
- positive strand, positive direction, looking for 5'-ATTTCAAA-3', 0.
- complement, negative strand, negative direction, looking for 5'-TAAAGTTT-3', 1, 5'-TAAAGTTT-3' at 1383.
- complement, negative strand, positive direction, looking for 5'-TAAAGTTT-3', 0.
- complement, positive strand, negative direction, looking for 5'-TAAAGTTT-3', 0.
- complement, positive strand, positive direction, looking for 5'-TAAAGTTT-3', 1, 5'-TAAAGTTT-3' at 2648.
- inverse complement, negative strand, negative direction, looking for 5'-TTTGAAAT-3', 0.
- inverse complement, negative strand, positive direction, looking for 5'-TTTGAAAT-3', 0.
- inverse complement, positive strand, negative direction, looking for 5'-TTTGAAAT-3', 0.
- inverse complement, positive strand, positive direction, looking for 5'-TTTGAAAT-3', 0.
- inverse negative strand, negative direction, looking for 5'-AAACTTTA-3', 0.
- inverse negative strand, positive direction, looking for 5'-AAACTTTA-3', 0.
- inverse positive strand, negative direction, looking for 5'-AAACTTTA-3', 0.
- inverse positive strand, positive direction, looking for 5'-AAACTTTA-3', 0.

## Eth negative direction (2596-1) distal promoters
- Positive strand, negative direction: ATTTCAAA at 1383.

## Eth positive direction (4050-1) distal promoters
- Negative strand, positive direction: ATTTCAAA at 2648.

## ERE random samplings
- EREr0: 0.
- EREr1: 0.
- EREr2: 0.
- EREr3: 0.
- EREr4: 0.
- EREr5: 0.
- EREr6: 0.
- EREr7: 0.
- EREr8: 1, ATTTCAAA at 934.
- EREr9: 0.
- EREr0ci: 0.
- EREr1ci: 0.
- EREr2ci: 0.
- EREr3ci: 0.
- EREr4ci: 0.
- EREr5ci: 0.
- EREr6ci: 0.
- EREr7ci: 0.
- EREr8ci: 0.
- EREr9ci: 0.

## EREr arbitrary negative direction (evens) (2596-1) distal promoters
- EREr8: ATTTCAAA at 934.

## EREr alternate positive direction (evens) (4050-1) distal promoters
- EREr8: ATTTCAAA at 934.

# ERE analysis and results
Ethylene responsive elements (ATTTCAAA).
Two ethylene response factors or ethylene responsive elements occur in the promoters of A1BG: positive strand, negative direction: ATTTCAAA at 1383 and negative strand, positive direction: ATTTCAAA at 2648. Both of these occur in the distal promoters on either side of A1BG. Sampling of ten random datasets looking for the ERE and its inverse complement found only one: ATTTCAAA at 934. Half way between ZSCAN22 and A1BG is about 2300 nucleotides and between ZNF497 and A1BG is about 2200. One is closer to ZSCAN22 and the other is closer to A1BG. The random occurrence is closer to the zinc finger than A1BG. The response element closer to A1BG is likely real even if inactive. As the real occurrences are more frequent and nearer mid points than the random result, it is likely that the EREs are not random but real and perhaps active.
Comparison:
The occurrences of real EREs are greater than the randoms. This suggests that the real EREs are likely active or activable.

# Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.