depar depar depar depar department of health and human ser tment of health and human ser tment of health and human ser tment of health and human ser tment of health and human services vices vices vices vices# Introduction
Each year, influenza virus infections cause substantial morbidity and mortality in the United States (1). Prevention of influenza relies primarily on vaccination. Until recently, only inactivated influenza vaccine administered by injection was available for use in the United States. Inactivated influenza vaccine is approved for persons aged >6 months, both with and without chronic medical conditions. In 2003, an intranasal, trivalent, cold-adapted, live, attenuated vaccine (LAIV) was newly approved for use among healthy persons aged 5-49 years. LAIV adds an option for vaccinating healthy persons aged 5-49 years who either want to avoid influenza or who have close contact with persons at high risk for experiencing serious complications from influenza infection.

# Description and Action Mechanisms of LAIV
LAIVs are in use in Russia and have been in development since the 1960s in the United States, where they have been evaluated as mono-, bi-, and trivalent formulations (2)(3)(4)(5)(6). The newly licensed LAIV is produced by MedImmune, Inc., (Gaithersburg, Maryland; ) and marketed under the name FluMistTM. It is a live, trivalent, intranasally administered vaccine that is - attenuated, producing mild or no signs or symptoms related to influenza virus infection; - temperature-sensitive, a property that limits the replication of the vaccine viruses at 38 o C-39 o C, and thus restricts LAIV viruses from replicating efficiently in human lower airways; and - cold-adapted, replicating efficiently at 25 o C, a temperature that is permissive for replication of LAIV viruses, but restrictive for replication of different wild-type viruses. In animal studies, LAIV viruses replicate in the mucosa of the nasopharynx, inducing protective immunity against The first step in developing an LAIV was the derivation of two stably attenuated master donor viruses (MDV), one for type A and one for type B influenza viruses. The two MDVs each acquired the cold-adapted, temperature-sensitive, attenuated phenotypes through serial passage in viral culture conducted at progressively lower temperatures. The vaccine viruses in LAIV are reassortant viruses containing genes from these MDVs that confer attenuation, temperature sensitivity, and cold adaptation and genes from the recommended contemporary wild-type influenza viruses, encoding the surface antigens hemagglutinin (HA) and neuraminidase (NA). Thus, MDVs provide the stably attenuated vehicles for presenting influenza HA and NA antigens, to which the protective antibody response is directed, to the immune system. The reassortant vaccine viruses are grown in embryonated hens' eggs. After the vaccine is formulated and inserted into individual sprayers for nasal administration, the vaccine must be stored at -15 o C or colder.
The immunogenicity of the approved LAIV has been assessed in multiple studies (7)(8)(9)(10)(11)(12)(13)(14)(15), which included approximately 100 children aged 5-17 years, and approximately 300 adults aged 18-49 years. LAIV virus strains replicate primarily in nasopharyngeal epithelial cells. The protective mechanisms induced by vaccination with LAIV are not completely understood but appear to involve both serum and nasal secretory antibodies. No single laboratory measurement closely correlates with protective immunity induced by LAIV.

# Comparison of LAIV with Inactivated Influenza Vaccine Major Similarities
LAIV and inactivated influenza vaccine contain strains of influenza viruses that are antigenically equivalent to the annually recommended strains: one influenza A (H3N2) virus, one A (H1N1) virus, and one B virus. Each year, one or more virus strains might be changed on the basis of global surveillance for influenza viruses and the emergence and spread of new strains. Viruses for both vaccines are grown in eggs. Both vaccines are administered annually to provide optimal pro-tection against influenza infection. This report includes a more detailed comparison of LAIV with inactivated influenza vaccine (Tables 1 and 2).

# Major Differences
Inactivated influenza vaccine contains killed viruses, whereas LAIV contains attenuated viruses still capable of replication. LAIV is administered intranasally by sprayer, whereas inactivated influenza vaccine is administered intramuscularly by injection. LAIV is more expensive than inactivated influenza vaccine. LAIV is approved for use only among healthy persons aged 5-49 years; inactivated influenza vaccine is approved for use among persons aged >6 months, including those who are healthy and those with chronic medical conditions. This report includes a more detailed comparison of LAIV with inactivated influenza vaccine (Tables 1 and 2).

# Efficacy and Effectiveness of LAIV Efficacy Among Healthy Children
A randomized, double-blind, placebo-controlled trial among 1,602 healthy children initially aged 15-71 months assessed the efficacy of the trivalent LAIV against culture-confirmed influenza during two seasons- (8,9). This trial included subsets of 238 healthy children (163 vaccinees and 75 placebo recipients) aged 60-71 months who received two doses and 74 children (54 vaccinees and 20 placebo recipients) aged 60-71 months who received a single dose during season one, and a subset of 544 children (375 vaccinees and 169 placebo recipients) aged 60-84 months during season two. Children who continued from season one to season two remained in the same study group. In season one, when vaccine and circulating virus strains were well-matched, efficacy was 93% for all participants, regardless of age, among subjects receiving 2 doses of LAIV. Efficacy was 87% in the 60-71-month subset for those who received 2 doses, and was 91% in the subset for those who received 1 or 2 doses. In season two, when the A (H3N2) component was not well-matched between vaccine and circulating virus strains, efficacy was 86% overall and 87% among those aged 60-84 months. The vaccine was 92% efficacious in preventing culture-confirmed influenza during the two-season study. Other results included a 27% reduction in febrile otitis media and a 28% reduction in otitis media with concomitant antibiotic use. Receipt of LAIV also resulted in decreased fever and otitis media among vaccine recipients who experienced influenza.  If not simultaneously administered, can be administered within 4 weeks of an inactivated vaccine Yes Yes - Populations at high risk from complications of influenza infection include persons aged >65 years; residents of nursing homes and other facilities that house persons with chronic medical conditions; adults and children with chronic disorders of the pulmonary or cardiovascular systems; adults and children with chronic metabolic diseases (including diabetes mellitus), renal dysfunction, hemoglobinopathies, or immunnosuppression; children and adolescents receiving long-term aspirin therapy (at risk for developing Reye syndrome after wild-type influenza infection); and women who will be in the second or third trimester of pregnancy during influenza season.  Immunosuppressed persons include, but are not limited to, those persons with human immunodeficiency virus, malignancy, or those receiving immunosuppressive therapies.  No data are available regarding effect on safety or efficacy.  Inactivated influenza vaccine coadministration with pneumococcal polysaccharide vaccine has been evaluated systematically only among adults.

# Effectiveness and Efficacy Among Healthy Adults
A randomized, double-blind, placebo-controlled trial among 4,561 healthy working adults aged 18-64 years assessed multiple endpoints, including reductions in illness, absenteeism, health-care visits, and medication use during peak and total influenza outbreak periods (16). The study was conducted during the 1997-98 influenza season, when the vaccine and circulating A (H3N2) strains were not well-matched. The study did not include laboratory virus testing of cases. Dur-ing peak outbreak periods, no difference was identified between LAIV and placebo recipients experiencing any febrile episodes. However, vaccination was associated with reductions in severe febrile illnesses of 19% and febrile upper respiratory tract illnesses of 24%. Vaccination also was associated with fewer days of illness, fewer days of work lost, fewer days with health-care provider visits, and reduced use of prescription antibiotics and over-thecounter medications.
Among the subset of 3,637 healthy adults aged 18-49 years, LAIV recipients (n = 2,411) had 26% fewer febrile upper respiratory illness episodes; 27% fewer lost work days as a result of febrile upper respiratory illness; and 18%-37% fewer days of health-care provider visits caused by febrile illness, compared with placebo recipients (n = 1,226). Days of antibiotic use were reduced by 41%-45% in this age subset. Another randomized, double-blind, placebo-controlled challenge study among 92 healthy adults (LAIV, n = 29; placebo, n = 31; inactivated influenza vaccine, n = 32) aged 18-41 years assessed the efficacy of both LAIV and trivalent inactivated vaccine (15). The overall efficacy of LAIV and inactivated influenza vaccine in preventing laboratory documented influenza from all three influenza strains combined was 85% and 71%, respectively, on the basis of experimental challenge by viruses to which study participants were susceptible before vaccination. The difference between the two vaccines was not statistically significant.

# Person-to-Person Transmission of Vaccine Viruses
Because LAIV contains live influenza viruses, a potential exists for transmission of these viruses from vaccinees to other persons. Vaccinated immunocompetent children can shed vaccine viruses for 1 virus strain, with a mean of 7.6 days duration (17). One influenza type B isolate was recovered from a placebo recipient and was confirmed to be vaccine-type virus. The estimated probability of acquiring vaccine virus after close contact with a single LAIV recipient was 0.58%-2.4%. The type B isolate retained the cold-adapted, temperature-sensitive, attenuated phenotype, and it possessed the same genetic sequence as a virus shed from a vaccine recipient in the same children's play group.

# Stability of Vaccine Viruses
In clinical trials, viruses shed by vaccine recipients have been phenotypically stable. In one study, nasal and throat swab specimens were collected from 17 study participants for 2 weeks after vaccine receipt (18). Virus isolates were analyzed by multiple genetic techniques. All isolates retained the LAIV genotype after replication in the human host, and all retained the cold-adapted and temperature-sensitive phenotypes.

# Recommendations for Influenza Vaccination
Recommendations for inactivated influenza vaccination have targeted specific groups for annual immunization, including persons aged >6 months who are at high risk for complications from influenza because of age or presence of certain medical conditions, persons who are in close contact with those at  These persons should receive inactivated influenza vaccine. high risk, persons aged 50-64 years, and close contacts of infants aged 0-6 months (1). Vaccination with inactivated influenza vaccine is also encouraged when feasible for children aged 6-23 months and their close contacts and caregivers. In addition, physicians should administer inactivated influenza vaccine to any person who wishes to reduce the likelihood of becoming ill with influenza. Recommendations for use of inactivated influenza vaccine are located at http:// www.cdc.gov/mmwr/PDF/rr/rr5208.pdf.

# Recommendations for Using Live, Attenuated Influenza Vaccine
LAIV is an option for vaccination of healthy persons aged 5-49 years, including persons in close contact with groups at high risk and those wanting to avoid influenza (Tables 1 and  2). Possible advantages of LAIV include its potential to induce a broad mucosal and systemic immune response, its ease of administration, and the acceptability of an intranasal rather than intramuscular route of administration (1).

# Persons Who Should Not Be Vaccinated with LAIV
The following populations should not be vaccinated with LAIV:

# Close Contacts of Persons at High Risk for Complications from Influenza
Close contacts of persons at high risk for complications from influenza should receive influenza vaccine to reduce transmission of wild-type influenza viruses to persons at high risk. No data are available assessing the risk for transmission of LAIV from vaccine recipients to immunosuppressed contacts. In the absence of such data, use of inactivated influenza vaccine is preferred for vaccinating household members, health-care workers, and others who have close contact with immunosuppressed persons because of the theoretical risk that a live, attenuated vaccine virus could be transmitted to the immunosuppressed person and cause disease. Otherwise, no preference is given to either inactivated influenza vaccine or LAIV for vaccination of healthy persons aged 5-49 years in close contact with all other groups at high risk.

# Timing of LAIV Administration
Administration of LAIV is not subject to tiered timing recommendations because it is not approved for use among populations at high risk. The optimal time to vaccinate is usually in October and November, but providers can begin vaccinating with LAIV as soon as vaccine supplies are available. Children aged 5-8 years who have never received influenza vaccine should receive LAIV for the first time in October or earlier because they need a second dose 6-10 weeks after the initial dose.

# Dosage, Administration, and Storage

# LAIV Dosage
LAIV is intended for intranasal administration only and should not be administered by the intramuscular, intradermal, or intravenous route. LAIV must be stored at -15 o C or colder. LAIV should not be stored in a frost-free freezer (because the temperature might cycle above -15 o C), unless a manufacturer-supplied freezer box is used. LAIV must be thawed before administration. This can be accomplished by holding an individual sprayer in the palm of the hand until thawed, with subsequent immediate administration. Alternatively, the vaccine can be thawed in a refrigerator and stored at 2 o C-8 o C for <24 hours before use. Vaccine should not be refrozen after thawing. LAIV is supplied in a prefilled singleuse sprayer containing 0.5 mL of vaccine. Approximately 0.25 mL (i.e., half of the total sprayer contents) is sprayed into the first nostril while the recipient is in the upright position. An attached dose-divider clip is removed from the sprayer to administer the second half of the dose into the other nostril. If the vaccine recipient sneezes after administration, the dose should not be repeated.
LAIV should be administered annually according to the following schedule:
- Children aged 5-8 years previously unvaccinated at any time with either LAIV or inactivated influenza vaccine should receive 2 doses  of LAIV separated by 6-10 weeks.  One dose equals 0.5 mL, divided equally between each nostril.
- Children aged 5-8 years previously vaccinated at any time with either LAIV or inactivated influenza vaccine should receive 1 dose of LAIV. They do not require a second dose. - Persons aged 9-49 years should receive 1 dose of LAIV. LAIV can be administered to persons with minor acute illnesses (e.g., diarrhea or mild upper respiratory tract infection with or without fever). However, if clinical judgment indicates nasal congestion is present that might impede delivery of the vaccine to the nasopharyngeal mucosa, deferral of administration should be considered until resolution of the illness.
Whether concurrent administration of LAIV with other vaccines affects the safety or efficacy of either LAIV or the simultaneously administered vaccine is unknown. In the absence of specific data indicating interference, following the ACIP general recommendations for immunization is prudent (19). Inactivated vaccines do not interfere with the immune response to other inactivated vaccines or to live vaccines. An inactivated vaccine can be administered either simultaneously or at any time before or after LAIV. Two live vaccines not administered on the same day should be administered >4 weeks apart when possible.

# LAIV Administration and Use of Influenza Antiviral Medications
The effect on safety and efficacy of LAIV coadministration with influenza antiviral medications has not been studied. However, because influenza antivirals reduce replication of influenza viruses, LAIV should not be administered until 48 hours after cessation of influenza antiviral therapy, and influenza antiviral medications should not be administered for 2 weeks after receipt of LAIV.

# LAIV Storage
LAIV must be stored at -15 o C or colder. LAIV should not be stored in a frost-free freezer because the temperature might cycle above -15 o C, unless a manufacturer-supplied freezer box or other strategy is used. LAIV may be thawed in a refrigerator and stored at 2 o C-8 o C for <24 hours before use. It should not be refrozen after thawing. Additional information is available at Wyeth Product Quality (1-800-411-0086) or at http:/ /www.FluMist.com.

# Side Effects and Adverse Reactions
Twenty prelicensure clinical trials assessed the safety of the approved LAIV. In these combined studies, approximately 28,000 doses of the vaccine were administered to >20,000 subjects. A subset of these trials were randomized, placebo-controlled studies in which >4,000 healthy children aged 5-17 years and >2,000 healthy adults aged 18-49 years were vaccinated. The incidence of adverse events possibly complicating influenza (e.g., pneumonia, bronchitis, bronchiolitis, or central nervous system events) was not statistically different among LAIV and placebo recipients aged 5-49 years.

# Children
Signs and symptoms reported more often among vaccine recipients than placebo recipients included runny nose or nasal congestion (20%-75%), headache (2%-46%), fever (0%-26%), and vomiting (3%-13%), abdominal pain (2%), and myalgias (0%-21%) (7,12,14,(20)(21)(22). These symptoms were associated more often with the first dose and were self-limited. In a subset of healthy children aged 60-71 months from one clinical trial (8,9), certain signs and symptoms were reported more often among LAIV recipients after the first dose (n = 214) than placebo recipients (n = 95) (e.g., runny nose, 48.1% versus 44.2%; headache, 17.8% versus 11.6%; vomiting, 4.7% versus 3.2%; myalgias, 6.1% versus 4.2%), but these differences were not statistically significant. Unpublished data from a study including subjects aged 1-17 years indicated an increase in asthma or reactive airways disease in the subset aged 12-59 months. Because of this, LAIV is not approved for use among children aged <60 months (see Recommendations for Using Live, Attenuated Influenza Vaccine).

# Adults
Among adults, runny nose or nasal congestion (28%-78%), headache (16%-44%), and sore throat (15%-27%) have been reported more often among vaccine recipients than placebo recipients (16,23,24). In one clinical trial (16), among a subset of healthy adults aged 18-49 years, signs and symptoms reported more frequently among LAIV recipients (n = 2,548) than placebo recipients (n = 1,290) within 7 days after each dose included cough (13.9% versus 10.8%); runny nose (44.5% versus 27.1%); sore throat (27.8% versus 17.1%); chills (8.6% versus 6.0%); and tiredness/weakness (25.7% versus 21.6%).

# Safety Among Groups at High Risk from Influenza-Related Morbidity
Until additional data are acquired, persons at high risk for experiencing complications from influenza infection (e.g., immunocompromised patients; patients with asthma, cystic fibrosis, or chronic obstructive pulmonary disease; or persons aged >65 years) should not be vaccinated with LAIV. Protection from influenza in these groups should be accomplished Online know what matters. e ncore.
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# Serious Adverse Events
Serious adverse events among healthy children aged 5-17 years or healthy adults aged 18-