B box gene transcriptions
"The mP2 EB fragment used for binding was the 118 nucleotide fragment extending from the Dde I site at position -140 to the Dde I site at position -23 . This fragment contains the GC, E, B, CAAT, and TATA boxes."

# Consensus sequences
TGGGCA is a B-box.
"The human  TGFB1 promoter region contains two binding sequences for  AP-1, designated AP-1 box A (TGACTCT) and box B (TGTCTCA), which mediate the upregulation of promoter activity via a PKC-dependent pathway after exposure of cells to a high-glucose environment (Refs 37, 38)."

# Hypotheses
- A1BG has neither a B-box (TGGGCA) nor a box B (or B1 box) (TGTCTCA) in either promoter.
- A1BG is not transcribed by either B box.
- Neither B box participates in the transcription of A1BG.

# B box (Johnson) samplings
Copying the above consensus B box and putting the sequences in "F" locates any consensus sequences in any nucleotide positions as may be found by the computer programs.
One 3'-TGGGCA-5' shows up at about -600 nucleotides from the TSS between ZSCAN22 and A1BG as A1BG is approached from ZSCAN22 on the positive strand. This warrants testing with the computer programs.
- Negative strand, negative direction: 0.
- Positive strand, negative direction: 9, TGGGCA at 4191, TGGGCA at 4040, TGGGCA at 3301, TGGGCA at 2773, TGGGCA at 2438, TGGGCA at 1359, TGGGCA at 1114, TGGGCA at 902, TGGGCA at 462.
- Negative strand, positive direction: 4, TGGGCA at 4180, TGGGCA at 2894, TGGGCA at 1945, TGGGCA at 27.
- Positive strand, positive direction: 0.
- Inverse complement, negative strand, negative direction: 0.
- Inverse complement, positive strand, negative direction: 4, TGCCCA at 4251, TGCCCA at 3883, TGCCCA at 3854, TGCCCA at 1458.
- Inverse complement, negative strand, positive direction: 2, TGCCCA at 3377, TGCCCA at 3237.
- Inverse complement, positive strand, positive direction: 1, TGCCCA at 3750.

## Bbox (4560-2846) UTRs
- Positive strand, negative direction: TGCCCA at 4251, TGGGCA at 4191, TGGGCA at 4040, TGCCCA at 3883, TGCCCA at 3854, TGGGCA at 3301.

## Bbox negative direction (2811-2596) proximal promoters
- Positive strand, negative direction: TGGGCA at 2773.

## Bbox positive direction (4265-4050) proximal promoters
- Negative strand, positive direction: TGGGCA at 4180.

## Bbox negative direction (2596-1) distal promoters
- Positive strand, negative direction: TGGGCA at 2438, TGCCCA at 1458, TGGGCA at 1359, TGGGCA at 1114, TGGGCA at 902, TGGGCA at 462.

## Bbox positive direction (4050-1) distal promoters
- Negative strand, positive direction: TGCCCA at 3377, TGCCCA at 3237, TGGGCA at 2894, TGGGCA at 1945, TGGGCA at 27.
- Positive strand, positive direction: TGCCCA at 3750.

# B box (Johnson) random dataset samplings
- Bboxr0: 1, TGGGCA at 1253.
- Bboxr1: 2, TGGGCA at 3675, TGGGCA at 2650.
- Bboxr2: 0.
- Bboxr3: 3, TGGGCA at 3857, TGGGCA at 3329, TGGGCA at 2535.
- Bboxr4: 1, TGGGCA at 4228.
- Bboxr5: 0.
- Bboxr6: 0.
- Bboxr7: 1, TGGGCA at 2615.
- Bboxr8: 2, TGGGCA at 2952, TGGGCA at 1018.
- Bboxr9: 1, TGGGCA at 3599.
- Bboxr0ci: 2, TGCCCA at 1080, TGCCCA at 245.
- Bboxr1ci: 0.
- Bboxr2ci: 0.
- Bboxr3ci: 5, TGCCCA at 4519, TGCCCA at 2177, TGCCCA at 1577, TGCCCA at 1423, TGCCCA at 136.
- Bboxr4ci: 0.
- Bboxr5ci: 1, TGCCCA at 2606.
- Bboxr6ci: 1, TGCCCA at 956.
- Bboxr7ci: 4, TGCCCA at 4059, TGCCCA at 3010, TGCCCA at 1406, TGCCCA at 1338.
- Bboxr8ci: 3, TGCCCA at 4140, TGCCCA at 1901, TGCCCA at 1380.
- Bboxr9ci: 2, TGCCCA at 1672, TGCCCA at 732.

## Bboxr arbitrary (evens) (4560-2846) UTRs
- Bboxr4: TGGGCA at 4228.
- Bboxr8: TGGGCA at 2952.
- Bboxr8ci: TGCCCA at 4140.

## Bboxr alternate (odds) (4560-2846) UTRs
- Bboxr1: TGGGCA at 3675.
- Bboxr3: TGGGCA at 3857, TGGGCA at 3329.
- Bboxr9: TGGGCA at 3599.
- Bboxr3ci: TGCCCA at 4519.
- Bboxr7ci: TGCCCA at 4059, TGCCCA at 3010.

## Bboxr alternate negative direction (odds) (2811-2596) proximal promoters
- Bboxr1: TGGGCA at 2650.
- Bboxr7: TGGGCA at 2615.
- Bboxr5ci: TGCCCA at 2606.

## Bboxr arbitrary positive direction (odds) (4265-4050) proximal promoters
- Bboxr7ci: TGCCCA at 4059.

## Bboxr alternate positive direction (evens) (4265-4050) proximal promoters
- Bboxr4: TGGGCA at 4228.
- Bboxr8ci: TGCCCA at 4140.

## Bboxr arbitrary negative direction (evens) (2596-1) distal promoters
- Bboxr0: TGGGCA at 1253.
- Bboxr8: TGGGCA at 1018.
- Bboxr0ci: TGCCCA at 1080, TGCCCA at 245.
- Bboxr6ci: TGCCCA at 956.
- Bboxr8ci: TGCCCA at 1901, TGCCCA at 1380.

## Bboxr alternate negative direction (odds) (2596-1) distal promoters
- Bboxr3: TGGGCA at 2535.
- Bboxr7: TGGGCA at 2615.
- Bboxr3ci: TGCCCA at 2177, TGCCCA at 1577, TGCCCA at 1423, TGCCCA at 136.
- Bboxr7ci: TGCCCA at 1406, TGCCCA at 1338.
- Bboxr9ci: TGCCCA at 1672, TGCCCA at 732.

## Bboxr arbitrary positive direction (odds) (4050-1) distal promoters
- Bboxr1: TGGGCA at 3675, TGGGCA at 2650.
- Bboxr3: TGGGCA at 3857, TGGGCA at 3329, TGGGCA at 2535.
- Bboxr7: TGGGCA at 2615.
- Bboxr9: TGGGCA at 3599.
- Bboxr3ci: TGCCCA at 2177, TGCCCA at 1577, TGCCCA at 1423, TGCCCA at 136.
- Bboxr5ci: TGCCCA at 2606.
- Bboxr7ci: TGCCCA at 3010, TGCCCA at 1406, TGCCCA at 1338.
- Bboxr9ci: TGCCCA at 1672, TGCCCA at 732.

## Bboxr alternate positive direction (evens) (4050-1) distal promoters
- Bboxr0: TGGGCA at 1253.
- Bboxr8: TGGGCA at 2952, TGGGCA at 1018.
- Bboxr0ci: TGCCCA at 1080, TGCCCA at 245.
- Bboxr6ci: TGCCCA at 956.
- Bboxr8ci: TGCCCA at 1901, TGCCCA at 1380.

# B-box analysis and results
TGGGCA is a B-box.
Comparison:
The occurrences of real B-boxes UTRs, proximals and negative direction distals are greater than the randoms, positive direction distals are greater than or equal to the randoms. This suggests that the real B-boxes are likely active or activable.

# B1 box (Sanchez) samplings
- Negative strand, negative direction: 2, TGTCTCA at 2445, TGTCTCA at 1075.
- Positive strand, negative direction: 5, TGTCTCA at 4373, TGTCTCA at 3323, TGTCTCA at 2033, TGTCTCA at 1089, TGTCTCA at 923.
- Negative strand, positive direction: 2, TGTCTCA at 2468, TGTCTCA at 2174.
- Positive strand, positive direction: 0.
- Negative strand, negative direction, inverse complement: 3, TGAGACA at 2029, TGAGACA at 1085, TGAGACA at 919.
- Positive strand, negative direction, inverse complement: 0.
- Positive strand, positive direction, inverse complement: 1, TGAGACA at 2308.
- Negative strand, positive direction, inverse complement: 0.

## B1 (4560-2846) UTRs
- Positive strand, negative direction: TGTCTCA at 4373, TGTCTCA at 3323.

## B1 negative direction (2811-2596) proximal promoters
- Negative strand, negative direction: TGTCTCA at 2445.

## B1 negative direction (2596-1) distal promoters
- Negative strand, negative direction: TGTCTCA at 2445, TGAGACA at 2029, TGAGACA at 1085, TGTCTCA at 1075, TGAGACA at 919.
- Positive strand, negative direction: TGTCTCA at 2033, TGTCTCA at 1089, TGTCTCA at 923.

## B1 positive direction (4050-1) distal promoters
- Negative strand, positive direction: TGTCTCA at 2468, TGTCTCA at 2174.
- Positive strand, positive direction: TGAGACA at 2308.

# B1 box (Sanchez) random dataset samplings
- B1boxr0: 0.
- B1boxr1: 0.
- B1boxr2: 0.
- B1boxr3: 0.
- B1boxr4: 0.
- B1boxr5: 0.
- B1boxr6: 0.
- B1boxr7: 0.
- B1boxr8: 0.
- B1boxr9: 0.
- B1boxr0ci: 1, TGAGACA at 4234.
- B1boxr1ci: 0.
- B1boxr2ci: 0.
- B1boxr3ci: 0.
- B1boxr4ci: 1, TGAGACA at 74.
- B1boxr5ci: 0.
- B1boxr6ci: 0.
- B1boxr7ci: 0.
- B1boxr8ci: 0.
- B1boxr9ci: 0.

## B1r arbitrary (evens) (4560-2846) UTRs
- B1boxr0ci: TGAGACA at 4234.

## B1r alternate positive direction (evens) (4265-4050) proximal promoters
- B1boxr0ci: TGAGACA at 4234.

## B1r arbitrary negative direction (evens) (2596-1) distal promoters
- B1boxr4ci: TGAGACA at 74.

## B1r alternate positive direction (evens) (4050-1) distal promoters
- B1boxr4ci: TGAGACA at 74.

# Box B (B1box) analysis and results
And "box B (TGTCTCA)  the upregulation of promoter activity via a PKC-dependent pathway after exposure of cells to a high-glucose environment (Refs 37, 38)."
Comparison:
The occurrences of real box Bs are greater than the randoms. This suggests that the real box Bs are likely active or activable.

# Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.
Initial content for this page in some instances came from Wikiversity.