ATA box gene transcription laboratory
Gene transcriptions are usually initiated by a transcription factor. The gene being considered in this laboratory is GeneID: 1, "A1BG alpha-1-B glycoprotein ".  The transcription factors are the ATA boxes.
A laboratory is a specialized activity where a student, teacher, or researcher can have hands-on, or as close to hands-on as possible, experience actively analyzing an entity, source, or object of interest.
Usually, expensive equipment, instruments, and/or machinery are available for taking the entity apart to see and accurately record how it works, what it's made of, and where it came from. This may involve simple experiments to test reality, collect data, and attempts to make some sense out of it.
Expensive equipment can be replaced or substituted for with more readily available tools.
This is another gene transcription laboratory to test the transcription of A1BG by the transcription factor: ATA boxes.

# Notations
You are free to create your own notation or use that already presented. A method to statistically assess your locator is also needed.

# Laboratory control group
A laboratory control group of some large number of laboratory test subjects or results may be used to define normal limits for the presence of an effect.

# Instructions
This laboratory is an activity for you to explore the universe for, to create a method for, or to examine. While it is part of the {{Gene project}}, it is also independent.
Some suggested entities to consider are
- available classification,
- human genes,
- eukaryotes,
- nucleotides,
- classical physics quantities,
- known gene expressions, or
- geometry.
More importantly, there are your entities.
You may choose to define your entities or use those already available.
Usually, research follows someone else's ideas of how to do something. But, in this laboratory you can create these too.
This is a gene project laboratory, but you may create what a laboratory, or a {{Gene project}} is.
This laboratory is structured.
I will provide an example. The rest is up to you.
Questions, if any, are best placed on the Discuss page.
To include your participation in each of these laboratories create a subpage of your user page once you register at Wikiversity and use this subpage, for example, your online name/laboratory effort.
Enjoy learning by doing!

# Hypotheses
- A1BG is not transcribed by an ATA box.
- ATA boxes have a role as downstream signal transducers in A1BG.
- ATA boxes may assist transcription of A1BG by other transcription factors.

# Introduction
The ATA box is a variant of the TATA box that appears in the globin and other genes. Instead of a sequence TATA as in the TATA box, the ATA box lacks the first thymine (T) and may be tissue specific.
"The 3' flanking area contained the highly conserved hexanucleotide sequence A-A-T-A-A-A found in eukaryotic messages between the terminator codon and the polyadenylylation site (44)."
"ATA boxes  can be clearly identified in the chicken A- and D-globin genes about 70 bp upstream from the initiator ATG codon  The sequences of the proposed cap sites agree with those determined for other globin genes (Fig. 6A; Refs. 15, 24, and 32) as do their positions relative to the ATA boxes"
An ATA box may have the sequence AAATAT. The CArG box has the sequence CCTATTATGG.
"The  ATA box, located 30 bp 5' to the putative cap site, is of the form AAATAAAA typically found in eutherian embryonic -like globin genes. In sequence comparisons with ATA boxes from human, mouse, and  adult and embryonic -like globin genes, the S.c- ATA box was found to most closely resemble that found in the D. virginiana -globin gene (Fig 4)."
This suggests a consensus sequence of 3'-AAATA(A/T)A-5' on the template strand, or perhaps 3'-(A/C/G/T)AATA(A/T)A-5'.

# Human genes
GeneID: 3043 HBB hemoglobin subunit beta, "The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'."

# Cystatin genes
The "four cystatin genes  contain the ATA-box sequence (ATAAA) in their 5'-flanking regions; however, the CAT-box sequence (CAT), a binding site of the transcription factor, CTF, is found only in the 5'-flanking region of the S-type cystatin genes."

# -thalassemia
"DNA sequence analysis of a cloned -globin gene from a Chinese patient with -thalassemia revealed a single nucleotide substitution (A G) within the ATA box homology and 28 base pairs upstream from the cap site."
"Comparison of the level of -globin transcripts in a variety of deletion mutants shows that for efficient transcription, both the ATA or GoldbergHogness box, and a region between 100 and 58 base pairs in front of the site at which transcription is initiated, are required. Deletion of either of these regions results in a decrease in the level of -globin transcripts by an order of magnitude; deletion of the ATA box causes an additional loss in the specificity of the site of initiation of RNA synthesis. The DNA sequences downstream from the ATA box, including the natural -globin mRNA cap site, are dispensable for transcription in vivo."
"The first is a sequence rich in the nucleic acids adenine and thymine (the Goldberg-Hogness, "TATA," or "ATA" box) which is located 20-30 base pairs upstream from the RNA initiation site (the cap site which is the transcriptional start site for the mRNA) and is characterized by a concensus sequence (5'-TATAA-ATA-3')."

# Core promoters
The core promoter is approximately -34 nts upstream from the TSS.
From the first nucleotide just after ZSCAN22 to the first nucleotide just before A1BG are 4460 nucleotides. The core promoter on this side of A1BG extends from approximately 4425 to the possible transcription start site at nucleotide number 4460.
From the first nucleotide just beyond ZNF497 to the first nucleotide just before A1BG are 4300 nucleotides. The core promoter on this side of A1BG extends from approximately 4266 to the possible transcription start site at nucleotide number 4300.
Def. "the factors, including RNA polymerase II itself, that are minimally essential for transcription in vitro from an isolated core promoter" is called the basal machinery, or basal transcription machinery.

# Proximal promoters
Def. a "promoter region  binds transcription factors that modify the affinity of the core promoter for RNA polymerase." is called a proximal promoter.
The proximal sequence upstream of the gene that tends to contain primary regulatory elements is a proximal promoter.
It is approximately 250 base pairs  upstream of the  start site.
The proximal promoter begins about nucleotide number 4210 in the negative direction.
The proximal promoter begins about nucleotide number 4150 in the positive direction.

# Distal promoters
The "upstream regions of the human CYP11A and bovine CYP11B genes  a distal promoter in each gene. The distal promoters are located at 1.8 to 1.5 kb in the upstream region of the CYP11A gene and 1.5 to 1.1 kb in the upstream region of the CYP11B gene."
"Using cloned chicken A-globin genes, either individually or within the natural chromosomal locus, enhancer-dependent transcription is achieved in vitro at a distance of 2 kb with developmentally staged erythroid extracts. This occurs by promoter derepression and is critically dependent upon DNA topology. In the presence of the enhancer, genes must exist in a supercoiled conformation to be actively transcribed, whereas relaxed or linear templates are inactive. Distal proteinprotein interactions in vitro may be favored on supercoiled DNA because of topological constraints."
Distal promoter regions may be a relatively small number of nucleotides, fairly close to the TSS such as (-253 to -54) or several regions of different lengths, many nucleotides away, such as (-2732 to -2600) and (-2830 to -2800).
The "istal promoter is not a spacer element."
Using an estimate of 2 knts, a distal promoter to A1BG would be expected after nucleotide number 2460.
If there are any transcription factors between ZN497 and A1BG, they are inside the gene for ZN497 as there are only 4300 nts between them to just beyond the other side of ZNF497.

# Samplings
Once you've decided on an entity, source, or object, compose a method, way, or procedure to explore it.
One way is to perceive (see, feel, hear, taste, or touch, for example) if there are more than one of them.
Ask some questions about it.
Does it appear to have a spatial extent?
Is there any change over time?
Can it be profiled with a kind of spectrum for example, by emitted radiation? Sample by plotting two or more apparent variables against each other, like intensity versus wavelength.
Is there some location, time, intensity, where there isn't one?
Regarding hypothesis 1: A1BG is not transcribed by an ATA box.
For the Basic programs (starting with SuccessablesATA.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand in the negative direction (from ZSCAN22 to A1BG) is SuccessablesATA--.bas, looking for 3'-AATAAA-5', 1, 3'-AATAAA-5', 1726,
- negative strand in the positive direction (from ZNF497 to A1BG) is SuccessablesATA-+.bas, looking for 3'-AATAAA-5', 1, 3'-AATAAA-5', 3427,
- positive strand in the negative direction is SuccessablesATA+-.bas, looking for 3'-AATAAA-5', 3, 3'-AATAAA-5', 3014, 3'-AATAAA-5', 3335, 3'-AATAAA-5', 4072,
- positive strand in the positive direction is SuccessablesATA++.bas, looking for 3'-AATAAA-5', 0,
- complement, negative strand, negative direction is SuccessablesATAc--.bas, looking for 3'-TTATTT-5', 3, 3'-TTATTT-5', 3014, 3'-TTATTT-5', 3335, 3'-TTATTT-5', 4072,
- complement, negative strand, positive direction is SuccessablesATAc-+.bas, looking for 3'-TTATTT-5', 0,
- complement, positive strand, negative direction is SuccessablesATAc+-.bas, looking for 3'-TTATTT-5', 1, 3'-TTATTT-5', 1726,
- complement, positive strand, positive direction is SuccessablesATAc++.bas, looking for 3'-TTATTT-5', 1, 3'-TTATTT-5', 3427,
- inverse complement, negative strand, negative direction is SuccessablesATAci--.bas, looking for 3'-TTTATT-5', 5, 3'-TTTATT-5', 3013, 3'-TTTATT-5', 3334, 3'-TTTATT-5', 4071, 3'-TTTATT-5', 4075, 3'-TTTATT-5', 4221,
- inverse complement, negative strand, positive direction is SuccessablesATAci-+.bas, looking for 3'-TTTATT-5', 2, 3'-TTTATT-5', 2347, 3'-TTTATT-5', 4142,
- inverse complement, positive strand, negative direction is SuccessablesATAci+-.bas, looking for 3'-TTTATT-5', 1, 3'-TTTATT-5', 4537,
- inverse complement, positive strand, positive direction is SuccessablesATAci++.bas, looking for 3'-TTTATT-5', 0,
- inverse, negative strand, negative direction, is SuccessablesATAi--.bas, looking for 3'-AAATAA-5', 1, 3'-AAATAA-5', 4537,
- inverse, negative strand, positive direction, is SuccessablesATAi-+.bas, looking for 3'-AAATAA-5', 0,
- inverse, positive strand, negative direction, is SuccessablesATAi+-.bas, looking for 3'-AAATAA-5', 5, 3'-AAATAA-5', 3013, 3'-AAATAA-5', 3334, 3'-AAATAA-5', 4071, 3'-AAATAA-5', 4075, 3'-AAATAA-5', 4221,
- inverse, positive strand, positive direction, is SuccessablesATAi++.bas, looking for 3'-AAATAA-5', 2, 3'-AAATAA-5', 2347, 3'-AAATAA-5', 4142.
Regarding hypothesis 2: ATA boxes have a role as downstream signal transducers in A1BG.
Regarding hypothesis 3: ATA boxes may assist transcription of A1BG by other transcription factors.

# Verifications
To verify that your sampling has explored something, you may need a control group. Perhaps where, when, or without your entity, source, or object may serve.
Another verifier is reproducibility. Can you replicate something about your entity in your laboratory more than 3 times. Five times is usually a beginning number to provide statistics (data) about it.
For an apparent one time or perception event, document or record as much information coincident as possible. Was there a butterfly nearby?
Has anyone else perceived the entity and recorded something about it?
Gene ID: 1, includes the nucleotides between neighboring genes and A1BG. These nucleotides can be loaded into files from either gene toward A1BG, and from template and coding strands. These nucleotide sequences can be found in Gene transcriptions/A1BG. Copying the above discovered ATA boxes and putting the sequences in "F" locates these sequences in the same nucleotide positions as found by the computer programs.

# Core promoters ATA boxes
From the first nucleotide just after ZSCAN22 to the first nucleotide just before A1BG are 4460 nucleotides. The core promoter on this side of A1BG extends from approximately 4425 to the possible transcription start site at nucleotide number 4460.
There are no ATA boxes on the negative strand, negative direction in the core promoter between 4425 and 4460.
There is the following inverse ATA box on the negative strand, negative direction: 1, 3'-AAATAA-5' at 4537.
From the first nucleotide just beyond ZNF497 to the first nucleotide just before A1BG are 4300 nucleotides. The core promoter on this side of A1BG extends from approximately 4266 to the possible transcription start site at nucleotide number 4300.
There are no ATA boxes in the positive direction in the core promoter between 4266 and 4300.

# Proximal promoter ATA boxes
The proximal promoter begins about nucleotide number 4210 in the negative direction.
There is the following inverse ATA box on the positive strand, negative direction: 3'-AAATAA-5' at 4221.
The proximal promoter begins about nucleotide number 4050 in the positive direction.
There is one inverse and inverse complement ATA box in the proximal promoter between 4050 and 4300: 3'-AAATAA-5' at 4142, and 3'-TTTATT-5' at 4142.

# Distal promoter ATA boxes
Using an estimate of 2 knts, a distal promoter to A1BG would be expected after nucleotide number 2460.
There is the following ATA box on the negative strand in the negative direction: 1, 3'-AATAAA-5' at 1726 nts from ZSCAN22.
There are the following ATA boxes on the positive strand in the negative direction: 3, 3'-AATAAA-5' at 3014, 3'-AATAAA-5' at 3335, and 3'-AATAAA-5' at 4072.
There are the following inverse ATA boxes on the positive strand, negative direction: 4, 3'-AAATAA-5' at 3013, 3'-AAATAA-5' at 3334, 3'-AAATAA-5' at 4071, 3'-AAATAA-5' at 4075.
There is the following ATA box on the negative strand in the positive direction: 1, 3'-AATAAA-5' at 3427. It has a complement on the positive strand in the positive direction: 1, 3'-TTATTT-5' at 3427.
There is another inverse complement ATA box on the negative strand in the positive direction in distal promoter: 3'-TTTATT-5' at 2347. It also has an inverse in the distal promoter: 3'-AAATAA-5' at 2347.

# Transcribed ATA boxes
The "four cystatin genes  contain the ATA-box sequence (ATAAA) in their 5'-flanking regions".
For "efficient transcription , both the ATA or Goldberg-Hogness box, and a region between 100 and 58 base pairs in front of the site at which transcription is initiated, are required."
The "DNA sequences downstream from the Goldberg-Hogness box are not necessary for specific in vitro transcription because a variety of mutants lacking sequences extending from -20 (or about 4 nucleotides to the 3' side of the ATA box) to as far as + 164 (in the small intron) are transcribed effectively in vitro.12"
"The promoter region of the gene contains an ATA box and a CCAAT box, which are located 32 and 74 bp upstream, respectively, from the transcription initiation site."
"The conserved ATA box fixes the initiation of transcription to a point 30 bp downstream from its position in the 5' flanking sequence.67,86,87"
"As is the case in BhO and phl, the ATA box in eucaryotic genes is typically located in an AT-rich region, approximately 30 bases from the mRNA cap site (Efstratiadis et al., 1980)."
"The putative promoter region of the mouse K19 gene is highly homologous to the corresponding sequences of the human and bovine K19 genes. It contains an ATA box, a CAAT box and two potential Sp1-binding sites."
"The analysis of the promoter region indicated that a putative ATA box is located 54 nucleotides upstream from the transcription start site".
"In the promoter region, an ATA box and a CCAAT box are located 32 and 74 base pairs (bp), respectively, upstream from the transcription initiation site."

# Laboratory reports
Below is an outline for sections of a report, paper, manuscript, log book entry, or lab book entry. You may create your own, of course.
ATA boxes transcription laboratory
by --Marshallsumter (discuss - contribs) 23:32, 18 November 2017 (UTC)

## Abstract
Three hypotheses regarding the possibility of ATA boxes being transcription factors for GeneID: 1 alpha-1-B glycoprotein (A1BG) have been examined: (1) A1BG is not transcribed by any ATA boxes, (2) ATA boxes have a role as downstream signal transducers in A1BG, and (3) ATA boxes may assist transcription of A1BG by other transcription factors. These have been tested by literature searching articles that report ATA boxes in the promoter region of a particular human gene and by using a simple computer program to look for ATA boxes in the nucleotide sequences on either side of the A1BG gene. Both the template DNA strand and the coding strand have been checked. To show that these ATA boxes can be used during or for transcription of A1BG at least one transcription factor has been found.

## Introduction
According to one source, A1BG is transcribed from the direction of ZNF497: 3' - 58864890: CGAGCCACCCCACCGCCCTCCCTTGG+1GGCCTCATTGCTGCAGACGCTCACCCCAGACACTCACTGCACCGGAGTGAGCGCGACCATCATG : 58866601-5', per Michael David Winther, Leah Christine Knickle, Martin Haardt, Stephen John Allen, Andre Ponton, Roberto Justo De Antueno, Kenneth Jenkins, Solomon O. Nwaka, and Y. Paul Goldberg, Fat Regulated Genes, Uses Thereof and Compounds for Mudulating Same, US Patent Office, July 29, 2004, at #v=onepage&q&f=false where the second 'G' at left of four Gs in a row is the TSS. Transcription was triggered in cell cultures and the transcription start site was found using reverse transcriptase. But, the mechanism for transcription is unknown.
Controlling the transcription of A1BG may have significant immune function against snake envenomation. A1BG forms a complex that is similar to those formed between toxins from snake venom and A1BG-like plasma proteins. These inhibit the toxic effect of snake venom metalloproteinases or myotoxins and protect the animal from envenomation.
Many transcription factors (TFs) may occur upstream and occasionally downstream of the transcription start site (TSS), in this gene's promoter. The following have been examined so far: (1) AGC boxes (GCC boxes), (2) CArG boxes, (3) enhancer boxes, (4) HY boxes, (5) metal responsive elements (MREs), and (6) STAT5s.
An AGC box was found in the distal promoter of either gene ZSCAN22 or A1BG on both the template and coding strands. But, as the only known transcription of A1BG occurs between Gene ID: 162968 ZNF497 and Gene ID: 1 A1BG, it is unlikely that this AGC box is naturally used to transcribe A1BG.
A full web search produced several references including a GeneCard for "zinc finger protein 497" and "GCC box", including "May be involved in transcriptional regulation." Zinc fingers are mentioned in association with GCC boxes in plants. It seems unlikely that an AGC box is involved in any way with the transcription of A1BG.
By combining a literature search with computer analysis of each promoter between ZSCAN22 and A1BG and ZNF497 and A1BG, CArG boxes have been found. To show that these CArG boxes may be used during or for transcription of A1BG at least one transcription factor has been affirmed.
A literature search of more recent results discovered: "Of the  FLC binding sites, 69% contained at least one CArG-box motif with the core consensus sequence CCAAAAAT(G/A)G and an AAA extension at the 3 end  other MADS-box flowering-time regulators, SOC1, SVP, and AGAMOUS-LIKE 24 (AGL24), bind to two different CArG-box motifs at 502 bp (CTAAATATGG) and 287 bp (CAATAATTGG) upstream of the translation start in the SEP3 gene (24), consistent with different specificities for the different MADS-box proteins."
These together with the core motif CC(A/T)6GG suggest a more general CArG-box motif of (C(C/A/T)(A/T)6(A/G)G). Subsequent computer-program testing revealed two more general CArG boxes: 3'-CAAAAAAAAG-5' at 1399 nts from ZSCAN22 and 3'-CATTAAAAGG-5' at 3441 nts from ZSCAN22, but none within 958 nts toward A1BG from ZNF497.
These results show that the presence of CArG boxes on the ZSCAN22 side of A1BG implies their use when transcribing A1BG, although they may be pointing toward ZSCAN22. These suggest that the hypothesis (A1BG is not transcribed by a CArG box) is false. Regarding the second hypothesis (The lack of a CArG box on either side of A1BG does not prove that it is not actively used to transcribe A1BG), the presence of more general CArG boxes in the distal promoter tentatively confirms this hypothesis.
CArG boxes do occur in the distal promoter of A1BG. And, it is likely that a CArG box is involved in some way with the transcription of A1BG.
The presence of many enhancer boxes on both sides of A1BG demonstrate that the hypothesis: "A1BG is not transcribed by an enhancer box", is false.
The finding by literature search of evidence verifying that at least one transcription factor can enhance or inhibit the transcription of A1BG using one or more enhancer boxes disproves the hypothesis: "Existence of an enhancer box on either side of A1BG does not prove that it is actively used to transcribe A1BG".
Enhancer boxes do occur in the proximal and distal promoters of A1BG. And, it is likely that an enhancer box is involved in some way with the transcription of A1BG.
HY boxes were not found in either core promoters or the proximal promoters in either direction. However, HY boxes were found in the distal promoters between ZSCAN22 and A1BG. No genes are described in the literature so far as transcribed from HY boxes in any distal promoters.
Either A1BG can be transcribed by HY boxes in the distal promoter, or A1BG is not transcribed by HY boxes. As the literature appears absent from a Google Scholar advanced search to confirm possible transcription from distal promoters, wet chemistry experiments are needed to test the possibility.
By combining a literature search with computer analysis of the promoter between ZSCAN22 and A1BG and ZNF497 and A1BG, metal responsive elements have been found. Literature search has also discovered at least three post-translational isoforms including the unaltered precursor. Although no metal responsive elements overlap any enhancer boxes in the distal promoter, there are elements in the distal promoter.
"The human genome is estimated to contain 700 zinc-finger genes, which perform many key functions, including regulating transcription.  clusters of zinc-finger genes  on human chromosome 19".
Nearby zinc-fingers on chromosome 19 include ZNF497 (GeneID: 162968), ZNF837 (GeneID: 116412), and ZNF8 (GeneID: 7554).
"In rodents and in humans, about one third of the zinc-finger genes carry the Kruppel-associated box (KRAB), a potent repressor of transcription (Margolin et al. 1994), . There are more than 200 KRAB-containing zinc-finger genes in the human genome, about 40% of which reside on chromosome 19 and show a clustered organization suggesting an evolutionary history of duplication events (Dehal et al. 2001)."
ZNF8 is in cluster V along with A1BG.
"In contrast to the four clusters considered , one that occurs at the telomere of chromosome 19, which we will call cluster V, has been very stable ."
"Apart from the somewhat unexpected location of Zfp35 on mouse chromosome 18 and of the AIBG orthologs on mouse chromosome 15 and rat chromosome 7, there has been little rearrangement."
So far no article has reported any linkage between zinc, including various zinc fingers, or cadmium, and A1BG.
Regarding additional isoforms, mention has been made of "new genetic variants of A1BG."
"Proteomic analysis revealed that  set of plasma proteins was  1 B-glycoprotein (A1BG) and its
post-translationally modified isoforms."
Pharmacogenomic variants have been reported. There are A1BG genotypes.
A1BG has a genetic risk score of rs893184.
"A genetic risk score, including rs16982743, rs893184, and rs4525 in F5, was significantly associated with treatment-related adverse cardiovascular outcomes in whites and Hispanics from the INVEST study and in the Nordic Diltiazem study (meta-analysis interaction P=2.39105)."
"rs893184 causes a histidine (His) to arginine (Arg)  substitution at amino acid position 52 in A1BG."
For example, GeneID: 9 has isoforms: a, b, X1, and X2. Each of these (a and b) have variants. Variants 1-6 and 9 all encode the same isoform (a).
Variants 7, 8 and 10 all encode isoform b. Isoforms X1 and X2 are predicted.
Variants can differ in promoters, untranslated regions, or exons. For GeneID: 9: This variant (1) represents the longest transcript but encodes the shorter isoform (a). This variant is transcribed from a promoter known as P1, promoter 2, or NATb promoter.
This variant (2, also known as Type IID) lacks an alternate exon in the 5' UTR, compared to variant 1. This variant is transcribed from a promoter known as P1, promoter 2, or NATb promoter.
This variant (9, also known as Type IA) has a distinct 5' UTR and represents use of an alternate promoter known as the NATa or P3 promoter, compared to variant 1.
But, A1BG in NCBI Gene lists only one isoform, the gene locus itself, and the protein transcribed is a precursor subject to translational or more likely post-translational modifications.
The presence of multiple MREs coupled with experimental results from the literature indicating post-translational isoforms tends to confirm the existence of two or more isoforms for A1BG.
Regarding hypothesis 1: STAT5s have a role as downstream signal transducers in A1BG.
The only known TSS for A1BG lies at 4300 nts from ZNF497 toward A1BG. A STAT5 transcription site lies at 3'-TTCCGGGAA-5' at 808 in the proximal promoter, i.e. from 800 (-58) to 808 (-50). This suggests that STAT5 assists in the transcription of A1BG.
"Computer analysis of the 2.3 kb rat a1bg promoter fragment revealed  Stat5  at  69/61 ."
The murine downstream promoter element is only 11 nts displaced from the human one. This suggests a STAT5 participation in human gene transcription of A1BG.
Regarding hypothesis 2: A1BG is not transcribed by any STAT5s.
"Computer analysis of the 2.3 kb rat a1bg promoter fragment revealed two putative Stat5 sites  at 2077/2069  69/61 ."
There are two STAT5s on the negative strand in the negative direction, 3'-AAGCAACTT-5' at 3506 (-954) and 3'-AAGGGACTT-5' at 3782 (-678) in the distal promoter between ZSCAN22 and A1BG. Although much closer than their likely murine counterparts, they are on the other side of A1BG from the STAT5 site confirming hypothesis 1. If active in humans or murine-like STAT5s occur within or beyond ZNF497 in this distal promoter, then human A1BG is transcribed using STAT5 promoters disproving hypothesis 2.
A Google Scholar search using ZNF497 with STAT5 found no articles discussing STAT5 sites inside or associated with ZNF497. To confirm they exist, a data file going say 3,000 nts away from A1BG into ZNF497 needs to be created and tested for a distal promoter on this side.
Regarding hypothesis 3: STAT5s may assist transcription of A1BG by other transcription factors.
Literature search has found that STAT5s assist transcription of A1BG by other transcription factors. The proximal STAT5 promoter is -58 to -50 from A1BG TSS. If another STAT5 promoter is at -2.3 kb, it is about -1.4 kb inside ZNF497 which is 3212 nts long. Per analogy to the rat this would be expected.
Per earlier laboratories transcription factors may occur in the distal promoters on the ZNF497 side of A1BG for
- AGC boxes,
- CArG boxes,
- Enhancer boxes,
- HY boxes,
- MREs and
- STAT5s.
The STAT5 promoter on the other side of A1BG (at about +3 kb is way beyond -2.1 through ZNF497 unless the DNA is folded to allow the STAT5 on the ZSCAN22 side to be used in analogy to the STAT5 on the same side as in the rat.
It isn't known which, if any, assist in locating and affixing the transcription mechanism for A1BG. This examination is the first to test one such DNA-occurring TF: the ATA boxes.

## Experiments
Computer programs were written and run on the positive and negative strands between ZSCAN22 or ZNF497 and A1BG.
Regarding hypotheses 1: A1BG is not transcribed by any ATA boxes.
Here, the experiments have two parts: (1) are there any ATA box promoters? and (2) are any of these used to transcribe A1BG?
The Basic programs (starting with SuccessablesATA.bas) were written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+) looking for 16 possible types of promoters.
Regarding hypothesis 2: ATA boxes have a role as downstream signal transducers in A1BG.
To test for possible downstream signal transducers, each program tested at least 100 nts downstream of each TSS.
Regarding hypothesis 3: ATA boxes may assist transcription of A1BG by other transcription factors.
Experiments consist literature searches which seek transcription of A1BG by ATA boxes and other transcription factors already found.

## Results
Regarding hypotheses 1: A1BG is not transcribed by any ATA boxes.
There are no ATA boxes on the negative strand, negative direction in the core promoter between 4425 and 4460.
There are no ATA boxes in the positive direction in the core promoter between 4265 and 4300.
There is the following inverse ATA box on the negative strand, negative direction in the proximal promoter: 3'-TTTATT-5', 4221.
There is one inverse and inverse complement ATA box in the proximal promoter in the positive direction between 4050 and 4300: 3'-AAATAA-5' at 4142, and 3'-TTTATT-5' at 4142.
There are the following complement ATA boxes on the negative strand, negative direction in the distal promoter: 3'-TTATTT-5', 3014, 3'-TTATTT-5', 3335, and 3'-TTATTT-5', 4072.
There are the following inverse ATA boxes on the negative strand, negative direction in the distal promoter: 3'-TTTATT-5', 3013, 3'-TTTATT-5', 3334, 3'-TTTATT-5', 4071, and 3'-TTTATT-5', 4075.
There is the following ATA box on the negative strand in the negative direction in the distal promoter: 1, 3'-AATAAA-5' at 1726 nts from ZSCAN22.
There is the following ATA box on the negative strand in the positive direction: 1, 3'-AATAAA-5' at 3427. It has a complement on the positive strand in the positive direction: 1, 3'-TTATTT-5' at 3427.
There is another inverse complement ATA box on the negative strand in the positive direction in distal promoter: 3'-TTTATT-5' at 2347. It also has an inverse in the distal promoter: 3'-AAATAA-5' at 2347.
"The conserved ATA box fixes the initiation of transcription to a point 30 bp downstream from its position in the 5' flanking sequence.67,86,87"
"As is the case in BhO and phl, the ATA box in eucaryotic genes is typically located in an AT-rich region, approximately 30 bases from the mRNA cap site (Efstratiadis et al., 1980)."
"The analysis of the promoter region indicated that a putative ATA box is located 54 nucleotides upstream from the transcription start site".
"In the promoter region, an ATA box and a CCAAT box are located 32 and 74 base pairs (bp), respectively, upstream from the transcription initiation site."
Regarding hypothesis 2: ATA boxes have a role as downstream signal transducers in A1BG.
There is the following inverse ATA box on the negative strand, negative direction: 1, 3'-AAATAA-5' at 4537.
"The promoter region of the gene contains an ATA box and a CCAAT box, which are located 32 and 74 bp upstream, respectively, from the transcription initiation site."
"The putative promoter region of the mouse K19 gene is highly homologous to the corresponding sequences of the human and bovine K19 genes. It contains an ATA box, a CAAT box and two potential Sp1-binding sites."
Regarding hypothesis 3: ATA boxes may assist transcription of A1BG by other transcription factors.
"The promoter region of the gene contains an ATA box and a CCAAT box, which are located 32 and 74 bp upstream, respectively, from the transcription initiation site."
For "efficient transcription , both the ATA or Goldberg-Hogness box, and a region between 100 and 58 base pairs in front of the site at which transcription is initiated, are required."

## Discussion
Regarding hypothesis 1:  A1BG is not transcribed by any ATA boxes.
There are no ATA boxes in the core promoter of A1BG from either direction or strand.
This is in disagreement with "The conserved ATA box fixes the initiation of transcription to a point 30 bp downstream from its position in the 5' flanking sequence.67,86,87", "As is the case in BhO and phl, the ATA box in eucaryotic genes is typically located in an AT-rich region, approximately 30 bases from the mRNA cap site (Efstratiadis et al., 1980)", and "In the promoter region, an ATA box and a CCAAT box are located 32 and 74 base pairs (bp), respectively, upstream from the transcription initiation site" for core promoter transcription.
There is the following inverse ATA box on the negative strand, negative direction in the proximal promoter: 3'-TTTATT-5', at 4221. This is some 239 nts from the TSS.
There is one inverse and inverse complement ATA box in the proximal promoter in the positive direction between 4050 and 4300: 3'-AAATAA-5' at 4142, and 3'-TTTATT-5' at 4142. As the TSS is at 4300 nts, this ATA box is some 158 nts away, where with the smaller data set 3'-TTTATT-5' was at 703. As the TSS is at 858 nts, this ATA box is some 155 nts away, which is approximately the same number of nts from the TSS but not close enough to be in the core promoter.
"The analysis of the promoter region indicated that a putative ATA box is located 54 nucleotides upstream from the transcription start site". This is not close enough to consider that this ATA box does contribute to transcription of A1BG on the negative strand in the positive direction.
There is another inverse complement ATA box on the negative strand in the positive direction in distal promoter: 3'-TTTATT-5' at 2347. It also has an inverse in the distal promoter: 3'-AAATAA-5' at 2347. This ATA box could be involved in A1BG transcription by approximate analogy to the rat involving STAT5s.
Regarding hypothesis 2: ATA boxes have a role as downstream signal transducers in A1BG.
There is the following inverse ATA box on the negative strand, negative direction: 3'-AAATAA-5' at 4537. On this strand, in this direction the TSS is at 4460 nts from ZSCAN22. This ATA box is 77 nts downstream. So far no published research has been found to verify this type of downstream promoter or enhancer ATA box. There may be another isoform TSS nearby.
Regarding hypothesis 3: ATA boxes may assist transcription of A1BG by other transcription factors.
"In the promoter region, an ATA box and a CCAAT box are located 32 and 74 base pairs (bp), respectively, upstream from the transcription initiation site."
"The promoter region of the gene contains an ATA box and a CCAAT box, which are located 32 and 74 bp upstream, respectively, from the transcription initiation site."
"The putative promoter region of the mouse K19 gene is highly homologous to the corresponding sequences of the human and bovine K19 genes. It contains an ATA box, a CAAT box and two potential Sp1-binding sites."
For "efficient transcription , both the ATA or Goldberg-Hogness box, and a region between 100 and 58 base pairs in front of the site at which transcription is initiated, are required."
Literature search has found that STAT5s assist transcription of A1BG by other transcription factors.
The proximal STAT5 promoter is -58 to -50 from A1BG TSS. But, none of the ATA boxes are close enough to any STAT5 promoter to match known transcription initiation.

## Conclusions
Regarding hypothesis 1: there are no ATA boxes in the core promoter of A1BG from either direction or strand.
This hypothesis has been shown to be true.
A corollary hypothesis might be 1.1: there are no ATA boxes in the proximal promoter of A1BG from either direction or strand.
This corollary hypothesis may be true. "The analysis of the promoter region indicated that a putative ATA box is located 54 nucleotides upstream from the transcription start site". There is one inverse and inverse complement ATA box in the proximal promoter in the positive direction between 4050 and 4300: 3'-AAATAA-5' at 4142, and 3'-TTTATT-5' at 4142. As the TSS is at 4300 nts, this ATA box is some 158 nts away, where with the smaller data set 3'-TTTATT-5' was at 703. As the TSS is at 858 nts, this ATA box is some 155 nts away, which is approximately the same number of nts from the TSS but not close enough to be in the core promoter and not 54 nts upstream from the TSS or to match other such genes with an ATA box.
But the ATA box at 2347 is likely involved in transcription of A1BG in analogy to the rat. Although this has not been confirmed as involved, the existence of this ATA box likely proves hypothesis 1 false.
Regarding hypothesis 2: ATA boxes have a role as downstream signal transducers in A1BG.
There is the following inverse ATA box on the negative strand, negative direction: 3'-AAATAA-5' at 4537. On this strand, in this direction the TSS is at 4460 nts from ZSCAN22. This ATA box is 77 nts downstream. So far no published research has been found to verify this type of downstream promoter or enhancer ATA box. There may be another isoform TSS nearby. As such, hypothesis 2 may be true.
Regarding hypothesis 3: ATA boxes may assist transcription of A1BG by other transcription factors.
This hypothesis has been shown by literature search to be true. But, none of the ATA boxes for A1BG are close enough to any STAT5 promoter to match known transcription initiation.

# Laboratory evaluations
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## Evaluation
No wet chemistry experiments were performed to confirm that Gene ID: 1 is transcribed from either side using ATA boxes, especially in the distal promoters or the downstream promoter. The NCBI database is generalized, whereas individual human genome testing could demonstrate that A1BG is transcribed from either side. It was a good check to add sufficient nts to the data sets for the ZNF497 side to disprove transcription of A1BG per analogy to the rat.