Serum response element gene transcriptions
The SRE wild type (SREwt) contains the nucleotide sequence ACAGGATGTCCATATTAGGACATCTGC, of which CCATATTAGG is the CArG box, TTAGGACAT is the C/EBP box, and CATCTG is the E box.
"Serum response factor (SRF) is an important transcription factor that regulates cardiac and skeletal muscle genes during development, maturation and adult aging . SRF regulates its target genes by binding to serum response elements (SREs), which contain a consensus CC(A/T)6GG (CArG) motif."
"The CCATAATAGG sequence in the SIRT2 gene promoter is a classic SRE, which allows the SRF dimer to firmly bind to the DNA sequence, whereupon various SRF-binding proteins may be recruited to form a protein complex, which in turn modulates the expression of the SIRT2 gene."

# Consensus sequences
In the nucleotide sequence ACAGGATGTCCATATTAGGACATCTGC,
5'-CCATATTAGG-3' is a CArG box that does not occur in either promoter of A1BG,
5'-CATCTG-3' is an E box that does not occur in either promoter of A1BG,
5'-TTAGGACAT-3' is a C/EBP box that does not occur in either promoter of A1BG using "F",
5'-ACAGGATGT-3' is contained in the above nucleotide sequence which has one occurring between ZNF497 and A1BG using "F" and none between ZSCAN22 and A1BG.

# SRE samplings
Copying a responsive elements consensus sequence ACAGGATGT and putting the sequence in "F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence ACAGGATGT (starting with SuccessablesSER.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for ACAGGATGT, 0.
- positive strand, negative direction, looking for ACAGGATGT, 0.
- negative strand, positive direction, looking for ACAGGATGT, 1, ACAGGATGT at 3575.
- positive strand, positive direction, looking for ACAGGATGT, 0.
- complement, negative strand, negative direction, looking for TGTCCTACA, 0.
- complement, positive strand, negative direction, looking for TGTCCTACA, 0.
- complement, positive strand, positive direction, looking for TGTCCTACA, 1, TGTCCTACA at 3575.
- complement, negative strand, positive direction, looking for TGTCCTACA, 0.
- inverse complement, negative strand, negative direction, looking for ACATCCTGT, 0.
- inverse complement, positive strand, negative direction, looking for ACATCCTGT, 0.
- inverse complement, negative strand, positive direction, looking for ACATCCTGT, 0.
- inverse complement, positive strand, positive direction, looking for ACATCCTGT, 0.
- inverse negative strand, negative direction, looking for TGTAGGACA, 0.
- inverse positive strand, negative direction, looking for TGTAGGACA, 0.
- inverse positive strand, positive direction, looking for TGTAGGACA, 0.
- inverse negative strand, positive direction, looking for TGTAGGACA, 0.

## SRE positive direction (4050-1) distal promoters
- Negative strand, positive direction: ACAGGATGT at 3575.

# SRE random dataset samplings
- SREr0: 0.
- SREr1: 0.
- SREr2: 0.
- SREr3: 0.
- SREr4: 0.
- SREr5: 0.
- SREr6: 0.
- SREr7: 0.
- SREr8: 0.
- SREr9: 0.
- SREr0ci: 0.
- SREr1ci: 0.
- SREr2ci: 0.
- SREr3ci: 0.
- SREr4ci: 0.
- SREr5ci: 0.
- SREr6ci: 0.
- SREr7ci: 0.
- SREr8ci: 0.
- SREr9ci: 0.

# SRE analysis and results
The SRE wild type (SREwt) contains the nucleotide sequence ACAGGATGT+CCATA+TTAGG+A+CAT+CTG+C, of which CCATATTAGG is the CArG box, TTAGGACAT is the C/EBP box, and CATCTG is the E box.
Comparison:
The occurrences of real SREs are greater than the randoms. This suggests that the real SREs are likely active or activable.

# Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.