EIN3 binding site gene transcriptions
"We scanned the ORE1 promoter and found a putative EIN3 binding site (EBS), ATGAACCT, located 1056~1064 bp upstream from the start codon (ATG) of the gene ."

# Human genes

# Gene expressions
EIN3 mat affect the gene expression of ORE1.

# Interactions

# Consensus sequences
"EIN3/EIL1 transcription factors were reported to bind to a consensus DNA sequence of AGACT ."

# Binding site for
A binding site for ethylene insensitive (EIN) 3 (EIN3) occurs in the oresara1 (ORE1) promoter.

# Promoter occurrences
ORE1 promoter has a putative EIN3 binding site.

# Hypotheses
- A1BG has no EIN3 binding sites in either promoter.
- A1BG is not transcribed by an EIN3 binding site.
- EIN3 binding site does not participate in the transcription of A1BG.

# EIN3 samplings
Copying a responsive elements consensus sequence ACGTACC and putting the sequence in "F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence A(C/T)G(A/T)A(C/T)CT (starting with SuccessablesEIN.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for A(C/T)G(A/T)A(C/T)CT, 1, ATGAACCT at 329, and complement.
- negative strand, positive direction, looking for A(C/T)G(A/T)A(C/T)CT, 0.
- positive strand, negative direction, looking for A(C/T)G(A/T)A(C/T)CT, 1, ATGAACCT at 1957, and complement.
- positive strand, positive direction, looking for A(C/T)G(A/T)A(C/T)CT, 0.
- complement, negative strand, negative direction, looking for T(A/G)C(A/T)T(A/G)GA, 1, TACTTGGA at 1957.
- complement, negative strand, positive direction, looking for T(A/G)C(A/T)T(A/G)GA, 0.
- complement, positive strand, negative direction, looking for T(A/G)C(A/T)T(A/G)GA, 1, TACTTGGA at 329.
- complement, positive strand, positive direction, looking for T(A/G)C(A/T)T(A/G)GA, 0.
- inverse complement, negative strand, negative direction, looking for AG(A/G)T(A/T)C(A/G)T, 0.
- inverse complement, negative strand, positive direction, looking for AG(A/G)T(A/T)C(A/G)T, 1, AGGTTCGT at 1928, and complement.
- inverse complement, positive strand, negative direction, looking for AG(A/G)T(A/T)C(A/G)T, 0.
- inverse complement, positive strand, positive direction, looking for AG(A/G)T(A/T)C(A/G)T, 1, AGGTACGT at 1935, and complement.
- inverse negative strand, negative direction, looking for TC(C/T)A(A/T)G(C/T)A, 0.
- inverse negative strand, positive direction, looking for TC(C/T)A(A/T)G(C/T)A, 1, TCCATGCA at 1935.
- inverse positive strand, negative direction, looking for TC(C/T)A(A/T)G(C/T)A, 0.
- inverse positive strand, positive direction, looking for TC(C/T)A(A/T)G(C/T)A, 1, TCCAAGCA at 1928.

## EIN3 negative direction (2596-1) distal promoters
- Negative strand, negative direction: ATGAACCT at 329.
- Positive strand, negative direction: ATGAACCT at 1957.

## EIN3 positive direction (4050-1) distal promoters
- Negative strand, positive direction: AGGTTCGT at 1928.
- Positive strand, positive direction: AGGTACGT at 1935.

# EIN3 binding site random dataset samplings
- EIN3r0: 0.
- EIN3r1: 0.
- EIN3r2: 1, ATGAACCT at 3145.
- EIN3r3: 0.
- EIN3r4: 1, ATGTACCT at 4544.
- EIN3r5: 2, ATGTACCT at 4396, ACGTACCT at 3129.
- EIN3r6: 0.
- EIN3r7: 0.
- EIN3r8: 1, ATGTACCT at 2781.
- EIN3r9: 0.
- EIN3r0ci: 0.
- EIN3r1ci: 1, AGGTACAT at 1128.
- EIN3r2ci: 0.
- EIN3r3ci: 1, AGGTTCGT at 4467.
- EIN3r4ci: 0.
- EIN3r5ci: 1, AGATTCGT at 4092.
- EIN3r6ci: 1, AGGTTCGT at 1273.
- EIN3r7ci: 0.
- EIN3r8ci: 0.
- EIN3r9ci: 0.

## EIN3r arbitrary (evens) (4560-2846) UTRs
- EIN3r2: ATGAACCT at 3145.
- EIN3r4: ATGTACCT at 4544.

## EIN3r alternate (odds) (4560-2846) UTRs
- EIN3r5: ATGTACCT at 4396, ACGTACCT at 3129.
- EIN3r3ci: AGGTTCGT at 4467.
- EIN3r5ci: AGATTCGT at 4092.

## EIN3r arbitrary positive direction (odds) (4445-4265) core promoters
- EIN3r5: ATGTACCT at 4396.

## EIN3r arbitrary negative direction (evens) (2811-2596) proximal promoters
- EIN3r8: ATGTACCT at 2781.

## EIN3r arbitrary positive direction (odds) (4265-4050) proximal promoters
- EIN3r5ci: AGATTCGT at 4092.

## EIN3r arbitrary negative direction (evens) (2596-1) distal promoters
- EIN3r6ci: AGGTTCGT at 1273.

## EIN3r alternate negative direction (odds) (2596-1) distal promoters
- EIN3r1ci: AGGTACAT at 1128.

## EIN3r arbitrary positive direction (odds) (4050-1) distal promoters
- EIN3r5: ACGTACCT at 3129.
- EIN3r1ci: AGGTACAT at 1128.

## EIN3r alternate positive direction (evens) (4050-1) distal promoters
- EIN3r2: ATGAACCT at 3145.
- EIN3r8: ATGTACCT at 2781.
- EIN3r6ci: AGGTTCGT at 1273.

# EIN3 analysis and results
"EIN3/EIL1 transcription factors were reported to bind to a consensus DNA sequence of  AGACT ."
Comparison:
The occurrences of real EIN3 distals are greater than the randoms. This suggests that the real EIN3s are likely active or activable.

# Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.