Gal4p gene transcriptions
"PGAL1 is often used repeatedly for the expression of different enzymes in the construction of metabolic pathways , so the gene copy number has to be increased to compensate for the insufficient promoter strength , and the utilization of too many galactose-inducible promoters may interfere with the metabolism of galactose due to the depletion of the transcription activator Gal4p ."
Galactose-inducible "promoters have various conserved UASGAL sites, a 17-bp consensus sequence 5-CCGNNNNNNNNNNNCGG-3 that is recognized by the transcription activator Gal4p to control their expression activities ."

# Human genes

# Interactions
"During the induction of galactose, the production of Gal4p can improve the PGAL1 activity 1000-fold ."
"We used the yeast Gal4p two-hybrid system (Fields and Sternglanz, 1994) to identify proteins that physically interact with GLD-1. We recovered two identical cDNAs in two-hybrid screens . One (OG2.3) using GLD-1 residues 84-341 and the other (CD13.1) using residues 273-457, both fused to the Gal4p DNA binding domain ."

# Consensus sequences
Upstream activating sequence for Gal4p is 5'-CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG-3' .

# Hypotheses
- A1BG has no Gal4ps in either promoter.
- A1BG is not transcribed by a Gal4p.
- Gal4p does not participate in the transcription of A1BG.

# Samplings
Copying 5'-CGGACCGC-3' in "F" yields none between ZSCAN22 and A1BG and none between ZNF497 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG (starting with SuccessablesGal4.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG, 0.
- negative strand, positive direction, looking for CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG, 0.
- positive strand, negative direction, looking for CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG, 0.
- positive strand, positive direction, looking for CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG, 0.
- complement, negative strand, negative direction, looking for GCC(C/T)NN(C/T)G(A/G)N(A/G)NGNGGC, 0.
- complement, negative strand, positive direction, looking for GCC(C/T)NN(C/T)G(A/G)N(A/G)NGNGGC, 0.
- complement, positive strand, negative direction, looking for GCC(C/T)NN(C/T)G(A/G)N(A/G)NGNGGC, 0.
- complement, positive strand, positive direction, looking for GCC(C/T)NN(C/T)G(A/G)N(A/G)NGNGGC, 0.
- inverse complement, negative strand, negative direction, looking for CGGNGN(A/G)N(A/G)G(C/T)NN(C/T)CCG, 0.
- inverse complement, negative strand, positive direction, looking for CGGNGN(A/G)N(A/G)G(C/T)NN(C/T)CCG, 0.
- inverse complement, positive strand, negative direction, looking for CGGNGN(A/G)N(A/G)G(C/T)NN(C/T)CCG, 0.
- inverse complement, positive strand, positive direction, looking for CGGNGN(A/G)N(A/G)G(C/T)NN(C/T)CCG, 0.
- inverse negative strand, negative direction, looking for GCCNCN(C/T)N(C/T)C(A/G)NN(A/G)GGC, 0.
- inverse negative strand, positive direction, looking for GCCNCN(C/T)N(C/T)C(A/G)NN(A/G)GGC, 0.
- inverse positive strand, negative direction, looking for GCCNCN(C/T)N(C/T)C(A/G)NN(A/G)GGC, 0.
- inverse positive strand, positive direction, looking for GCCNCN(C/T)N(C/T)C(A/G)NN(A/G)GGC, 0.

# Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.