Myocyte enhancer factor gene transcriptions
Mef2 was originally identified as a transcription factor complex through promoter analysis of the muscle creatine kinase (mck) gene to identify nuclear factors interacting with the mck enhancer region during muscle differentiation.
Three human mRNA coding sequences designated RSRF (Related to Serum Response Factor) were cloned and shown to dimerize, bind a consensus sequence similar to the one present in the MCK enhancer region, and drive transcription. RSRFs were subsequently demonstrated to encode human genes now named Mef2A, Mef2B and Mef2D.
Myocyte enhancer factor-2 (MEF2) proteins are a family of transcription factors which through control of gene expression are important regulators of cellular differentiation and consequently play a critical role in embryonic development. In adult organisms, Mef2 proteins mediate the stress response in some tissues.

# Human genes
Vertebrates have at least four versions of the Mef2 gene (human versions are denoted as Myocyte-specific enhancer factor 2A (MEF2A), MEF2B, MEF2C, and MEF2D), all expressed in distinct but overlapping patterns during embryogenesis through adulthood.

# Interactions

# Consensus sequences
"The current study delineates the conformational paradigm, clustered recognition, and comparative DNA binding preferences for MEF2A and MEF2B-specific MADS-box/MEF2 domains at the YTA(A/T)4TAR consensus motif." Y = (C/T) and R = (A/G). The consensus sequence is (C/T)TA(A/T)(A/T)(A/T)(A/T)TA(A/G).

# Myocyte enhancer factor samplings
Copying an apparent consensus sequence for the TTATAT or CTAATT and putting it in "F" finds two (TTATAT) located between ZSCAN22 and one (CTAATT) between ZNF497 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence (C/T)TA(A/T)(A/T)(A/T)(A/T)TA(A/G) (starting with SuccessablesMYO.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for (C/T)TA(A/T)(A/T)(A/T)(A/T)TA(A/G), 2, TTATTATTAA at 4226, CTATATATAA at 1601.
- positive strand, negative direction, looking for (C/T)TA(A/T)(A/T)(A/T)(A/T)TA(A/G), 0.
- positive strand, positive direction, looking for (C/T)TA(A/T)(A/T)(A/T)(A/T)TA(A/G), 1, CTAATATTAA at 4169.
- negative strand, positive direction, looking for(C/T)TA(A/T)(A/T)(A/T)(A/T)TA(A/G), 1, CTAATTTTAA at 2443.
Inverse complement is the same as the direct myocyte enhancer factor.

## MEF UTR gene transcriptions
- Negative strand, negative direction: TTATTATTAA at 4226.

## MEF proximal promoters
- Positive strand, positive direction: CTAATATTAA at 4169.

## MEF distal promoters
- Negative strand, negative direction: CTATATATAA at 1601.
- Negative strand, positive direction: CTAATTTTAA at 2443.

# Myocyte enhancer factor random dataset samplings
- MYOr0: 0.
- MYOr1: 0.
- MYOr2: 2, CTAAATTTAG at 2521, TTAAATTTAG at 1772.
- MYOr3: 0.
- MYOr4: 2, TTAATATTAA at 4164, TTATATTTAA at 3724.
- MYOr5: 0.
- MYOr6: 1, TTAAATATAA at 2239.
- MYOr7: 1, CTATAAATAA at 3632.
- MYOr8: 0.
- MYOr9: 0.
- MYOr0ci: 0.
- MYOr1ci: 0.
- MYOr2ci: 2, CTAAATTTAG at 2521, TTAAATTTAG at 1772.
- MYOr3ci: 0.
- MYOr4ci: 2, TTAATATTAA at 4164, TTATATTTAA at 3724.
- MYOr5ci: 0.
- MYOr6ci: 1, TTAAATATAA at 2239.
- MYOr7ci: 1, CTATAAATAA at 3632.
- MYOr8ci: 0.
- MYOr9ci: 0.

## MYOr arbitrary (evens) (4560-2846) UTRs
- MYOr4: TTAATATTAA at 4164, TTATATTTAA at 3724.

## MYOr alternate (odds) (4560-2846) UTRs
- MYOr7: CTATAAATAA at 3632.

## MYOr alternate positive direction (evens) (4265-4050) proximal promoters
- MYOr4: TTAATATTAA at 4164.

## MYOr arbitrary negative direction (evens) (2596-1) distal promoters
- MYOr2: CTAAATTTAG at 2521, TTAAATTTAG at 1772.
- MYOr6: TTAAATATAA at 2239.

## MYOr arbitrary positive direction (odds) (4050-1) distal promoters
- MYOr7: CTATAAATAA at 3632.

## MYOr alternate positive direction (evens) (4050-1) distal promoters
- MYOr2: CTAAATTTAG at 2521, TTAAATTTAG at 1772.
- MYOr4: TTATATTTAA at 3724.
- MYOr6: TTAAATATAA at 2239.

# Myocyte enhancer factor analysis and results
"The current study delineates the conformational paradigm, clustered recognition, and comparative DNA binding preferences for MEF2A and MEF2B-specific MADS-box/MEF2 domains at the YTA(A/T)4TAR consensus motif."
Comparison:
The occurrences of real myocyte enhancer factors are greater than the randoms. This suggests that the real myocyte enhancer factors are likely active or activable.