Wogonoside inhibits angiogenesis in breast cancer via suppressing Wnt/β-catenin pathway
Authors: Na Lu
Kai Zhao
Zhiyu Li
Xiaorui Li
Yang Hu
Xuwei Luo
Yuxin Zhou
Qidong You
Libin Wei
Qinglong Guo
Yujie Huang
Journal: Molecular Carcinogenesis (2016)
Discipline: CLINICAL MEDICINE
Keywords: Wnt/β-catenin; angiogenesis; breast cancer; wogonoside.
Affiliations: Jiangsu Key Laboratory of Carcinogenesis and Intervention, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, People's Republic of China., Department of Medicinal Chemistry, China Pharmaceutical University, Nanjing, People's Republic of China., Jiangsu Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing, People's Republic of China., Jiangsu Key Laboratory of Carcinogenesis and Intervention, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, People's Republic of China. .

Abstract:  Wogonoside, a main flavonoid component derived from the root of Scutellaria baicalensis Georgi, has been reported to have anti-angiogenesis and anti-leukemia activities. However, whether it can inhibit tumor angiogenesis is unclear. In this study, we investigate the inhibitory effect of wogonoside on angiogenesis in breast cancer and its underlying mechanisms. ELISA assay shows that wogonoside (25, 50, and 100 µM) decreases the secretion of VEGF in MCF-7 cells by 30.0%, 35.4%, and 40.1%, respectively. We find it inhibits angiogenesis induced by the conditioned media from MCF-7 cells in vitro and in vivo by migration, tube formation, rat aortic ring, and chicken chorioallantoic membrane (CAM) assay. Meanwhile, wogonoside can inhibit the growth and angiogenesis of MCF-7 cells xenografts in nude mice. The reduction of tumor weight can be found both in wogonoside (80 mg/kg) and bevacizumab (20 mg/kg) treated group, and the tumor inhibition rate is 42.1% and 48.7%, respectively. In addition, mechanistic studies demonstrate that wogonoside suppresses the activation of Wnt/β-catenin pathway in MCF-7 cells. Wogonoside (100 µM) decreases the intracellular level of Wnt3a, increases the expression of GSK-3β, AXIN, and promotes the phosphorylation of β-catenin for proteasome degradation significantly. Furthermore, the nuclear accumulation of β-catenin and the DNA-binding activity of β-catenin/TCF/Lef complex are inhibited by 49.2% and 28.7%, respectively, when treated with 100 µM wogonoside. Taken together, our findings demonstrate that wogonoside is a potential inhibitor of tumor angiogenesis and can be developed as a therapeutic agent for breast cancer. © 2015 Wiley Periodicals, Inc. 

Retracted: No

PubPeer comments:
 In 2014, Alan Welch presented his work along with Emma Knight to the AIS where they looked into a method of statistics called Magnitude Based Inference (MBI). MBI has never been published in a statistical or mathematical journal but is heavily used by the sports science field. You can view the presentation here https: maths.anu.edu.au files AIS-slides.pdf . After presenting MBI was supposedly discontinued at the AIS after Alan and Emma showed the method to be fundamentally flawed. In 2018 a 538 article was written about MBI uptake within the sports science field. https: fivethirtyeight.com features how-shoddy-statistics-found-a-home-in-sports-research This article notes to further work was done by Kristin Sainani, showing that MBI has numerous issues. She gives reproducible mathematics to show this. https: www.ncbi.nlm.nih.gov pubmed 29683920 In 2019 there was an article written in The Age talking about how still MBI is being used. https: www.smh.com.au national cold-water-poured-on-scientific-studies-based-on-statistical-cult-20191108-p538t6.html This is yet another MBI paper post MBI being banned as a method by the countries institute of sport. ,