Long Noncoding RNA LINC00472 Inhibits Proliferation and Promotes Apoptosis of Lung Adenocarcinoma Cells via Regulating miR-24-3p/ DEDD
Authors: Daping Yu
Xu Cheng
Chongyu Su
Kang Shi
Yi Han
Yunsong Li
Zhidong Liu
Journal: Technology in Cancer Research & Treatment (2018)
Discipline: CLINICAL MEDICINE
Keywords: DEDD; LINC00472; lung adenocarcinoma; miR-24-3p.
Affiliations: 1 Department of Thoracic Surgery, Beijing Chest Hospital, Beijing Tuberculosis and Thoracic Tumor Research Institute, Capital Medical University, Beijing, China.

Abstract:  Objective: We aimed to detect the role of LINC00472 via regulating miR-24-3p and death effector domain-containing DNA-binding protein in lung adenocarcinoma. Methods: Long noncoding RNA, microRNA, and messenger RNA levels were determined using reverse transcription quantitative polymerase chain reaction. The expression of death effector domain-containing DNA-binding protein was determined using Western blot assay. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and colony formation assay were conducted to explore the proliferation of cells. The cell apoptosis was tested by flow cytometry assay. Target relationships between miR-24-3p, death effector domain-containing DNA-binding protein, and LINC00472 were validated by dual-luciferase reporter gene assay. Results: LINC00472 and death effector domain-containing DNA-binding protein were found to be underexpressed, whereas miR-24-3p was found overexpressed in lung adenocarcinoma cell lines and tissues. Both LINC00472 and death effector domain-containing DNA-binding protein can bind to miR-24-3p. Overexpression of LINC00472 led to higher death effector domain-containing DNA-binding protein level, demoting cell proliferation while promoting apoptosis. Overexpression of miR-24-3p reduced death effector domain-containing DNA-binding protein level, which facilitated cell proliferation and inhibited cell apoptosis, as well as to some extent restrained the effects of LINC00472. The high expression of miR-24-3p in tumor cells was negatively related to LINC00472 and death effector domain-containing DNA-binding protein, whereas the expression of LINC00472 and that of death effector domain-containing DNA-binding protein were positively correlated. Conclusion: Our findings suggested that LINC00472 contributed to the increase in lung adenocarcinoma cell apoptosis and the inhibition of proliferation via regulating miR-24-3p/ DEDD, which might provide a novel insight into potential therapeutic approach for lung adenocarcinoma. 

Retracted: No

PubPeer comments:
 Figure 3C. * Red circles: In the third column from the left, both wells look very similar to each other. It is not a simple duplication, however, because the two photos differ in the presence of a thick blue colony roughly in the center of the well, as indicated with a thin rounded box. ![file] , 
 Figures 4A and 6A. * Several panels appear to look more similar to each other than expected. Boxes of the same color show panels that look quite similar. Of note, although the panels look similar, the labels suggest that these are different samples, and the gated percentages are different. ![file] , 
 **Retracted**, July 26, 2022: https: doi.org 10.1177 15330338221109184  *At the request of the journal Executive Editor the following article has been retracted.* *Su C, Shi K, Cheng X, et al. Long Noncoding RNA LINC00472 Inhibits Proliferation and Promotes Apoptosis of Lung Adenocarcinoma Cells via Regulating miR-24-3p DEDD. Technology in Cancer Research Treatment. January 2018. doi:10.1177 1533033818790490* *The Executive Editor became aware of several potential image duplication and manipulation issues raised by a reader and also noted on PubPeer. Specifically:* - *Figure 3C pCDNA3.1+miR-24-3p mimics wells appear to be duplicated. The bottom image appears to have an addition of cells in the center of the image.* - *Figure 4A H1299 Mock FACS plot appears similar to Figure 4A H460 Mock FACS plot and Figure 4A H1299 pCDNA3.1+NC FACS plot* - Figure 4A H460 pCDNA3.1+NC FACS plot appears similar to Figure 6A H1299 Mock FACS plot and Figure 6A H1299 pCDNA3.1 FACS plot* *The authors did not provide a sufficient response and did not respond to journal requests for underlying data to resolve the concerns. As a result, the scientific accuracy of this paper is not reliable.* *Adhering to the international guidelines established by the Committee on Publication Ethics, the Journal has determined these are grounds for retraction.* *The authors did not respond or could not be reached.* ,