Downregulated long non-coding RNA LINC00899 inhibits invasion and migration of spinal ependymoma cells via RBL2-dependent FoxO pathway
Authors: Liang Han
Nan Tian
Yao Fu
Yu Tian
Qun-Bang Chen
Zhao-Hui Li
Ning-Ning Lv
Journal: Cell Cycle (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: FoxO pathway; LINC00899; RBL2; invasion; migration
Affiliations: Department of Neurosurgery, China-Japan Union Hospital of Jilin University, Changchun, P.R. China, Department of Nuclear Medicine, China-Japan Union Hospital of Jilin University, Changchun, P.R. China, College of Life Science, Zhejiang Chinese Medical University, Hangzhou, P.R. China, Department of Pathology, China-Japan Union Hospital of Jilin University, Changchun, P.R. China

Abstract:  This study is aimed to clarify the potential role of lncRNA LINC00899 in invasion and migration of spinal ependymoma cells through the FoxO pathway via RBL2. Spinal ependymoma related chip data (GSE50161 and GSE66354) was initially downloaded and differentially expressed lncRNAs were screened out. Fifty-eight cases of spinal ependymoma and normal ependymal tissues were collected. The effects of LINC00899 and RBL2 on the spinal ependymoma cell migration and invasion were determined using the third generation spinal ependymoma cells and transfection with LINC00899 vector, siRNA-LINC00899 and siRNA-RBL2. The expression of LINC00899, pathway and cell proliferation- and apoptosis-related factors was determined. Finally, we also detected cell proliferation, migration, invasion, cycle and apoptosis after transfection. Our results showed that LINC00899 was up-regulated in spinal ependymoma and RBL2 was confirmed as a target gene of LINC00899 and found to be involved in regulation of FoxO pathway. LINC00899 expression increased in spinal ependymoma tissues whereas RBL2 expression decreased. Moreover, we found that siRNA-LINC00899 could elevate RBL2, p21, p27 and Bax levels, decrease FoxO, Bcl-2, Vimentin, Annexin levels, reduced cell proliferation, migration and invasion and enhanced apoptosis. Taken together, our study suggests that down-regulated LINC00899 exerts anti-oncogenic effects on spinal ependymoma via RBL2-dependent FoxO, which provides a novel therapeutic target for the treatment of spinal ependymomas. 

Retracted: Yes

PubPeer comments:
 Fig 3a.  Immunohistochemical staining of RBL2 in the normal group and the spinal ependymoma group (400 u00d7); the positive cells is mainly located in the nucleus with a small amount of expression in the cytoplasm Well done Cell Cycle reviewers. ![file] , 
 Retracted [15 July 2022] . --------------------- We, the Editors and Publisher of the journal *Cell Cycle*, have retracted the following article: Qun-Bang Chen, Zhao-Hui Li, Yao Fu, Ning-Ning Lv, Nan Tian, Liang Han, and Yu Tian. Downregulated long non-coding RNA LINC00899 inhibits invasion and migration of spinal ependymoma cells via RBL2-dependent FoxO pathway. Cell Cycle. 2019;18(19):2566u20132579. doi: 10.1080 15384101.2019.1652046 Since publication, concerns have been raised about the integrity of the data in the article. When approached for an explanation, the authors have been unable to address the concerns raised and have not been able to provide their original data. As verifying the validity of published work is core to the integrity of the scholarly record, we are therefore retracting the article. The corresponding author listed in this publication has been informed. We have been informed in our decision-making by our policy on publishing ethics and integrity and the COPE guidelines on retractions. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as u2018Retractedu2019. ,