microRNA-98 protects sepsis mice from cardiac dysfunction, liver and lung injury by negatively regulating HMGA2 through inhibiting NF-κB signaling pathway
Authors: Shaodong Yang
Zhixia Zhu
Xingyu Lin
Jingfa Zhu
Cairong Yan
Journal: Cell Cycle (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: HMGA2; MicroRNA-98; sepsis.
Affiliations: Department of Emergency, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou, China, Department of Emergency, Fujian Medical University Union Hospital, Fuzhou, China

Abstract:  Recently, MicroRNA-98 (miR-98) works as a biomarker in some diseases, such as lung cancer, Schizophrenia, and breast cancer, but there still lack of studies on the function of miR-98 during sepsis. Thus, our study is conducted to figure out the function of miR-98 for the regulation of cardiac dysfunction, liver and lung injury in sepsis mice. Cecum ligation and puncture was used to establish the sepsis mice model. Next, miR-Con and agomiR-98 were injected into the tail vein of mice 48 h after modeling. Then, expression of miR-98, HMGA2, NF-κB, inflammatory factors, apoptosis-related proteins in myocardial, liver and lung tissues of septic mice were determined. Moreover, other indices that were associated with cardiac dysfunction, liver and lung injury in septic mice were detected. Finally, bioinformatics analysis and luciferase activity assay were utilized to validate the binding site between miR-98 and HMGA2. miR-98 was poorly expressed, while HMGA2, NF-κB pathway-related proteins were highly expressed in myocardial, liver, and lung tissues of mice with sepsis. Upregulated miR-98 inhibited HMGA2, NF-κB, TNF-α, IL-6, Bcl-2 and increased IL-10, Cleaved caspase-3 and Bax expression in myocardial, liver, and lung tissues of septic mice. Upregulation of miR-98 decreased LVEDP, CTn-I, BNP, ALT, AST, TBIL, LDH, and PaCO2 while increased +dp/dt max, -dp/dt max, pH and PaO2 in sepsis mice. miR-98 was a direct target gene of HMGA2. Our study provides evidence that miR-98 protects sepsis mice from cardiac dysfunction, liver and lung injury by negatively mediating HMGA2 via the inhibition of the NF-κB signaling pathway. 

Retracted: No

PubPeer comments:
 ![file] ![file] , 
 The previous comment was made on 16 February 2020. No response from the authors? ![] , 
 The treatment of Fig 3b is extraordinary, and deserves its own comment. ![file] , 
 Unfortunately, this is apparently common practice for the publisher Taylor Francis. They share no responsibility for the content of their publications. They know that Cell cycle is totally infiltrated by the fabrications from the different papermills from China, but there are apparently no initiativ to stop this terrible mass disinformation. , 
 Retracted [15 July 2022] . -------------- We, the Editors and Publisher of the journal *Cell Cycle*, have retracted the following article: Jingfa Zhu, Xingyu Lin, Cairong Yan, Shaodong Yang, and Zhixia Zhu. microRNA-98 protects sepsis mice from cardiac dysfunction, liver and lung injury by negatively regulating HMGA2 through inhibiting NF-u03baB signaling pathway. Cell Cycle. 2019;18(16):1948u20131964. doi: 10.1080 15384101.2019.1635869 Since publication, significant concerns have been raised about the integrity of the data and reported results in the article. When approached for an explanation, the authors did not provide their original data or any necessary supporting information. As verifying the validity of published work is core to the integrity of the scholarly record, we are therefore retracting the article. The corresponding author listed in this publication has been informed. We have been informed in our decision-making by our policy on publishing ethics and integrity and the COPE guidelines on retractions. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as u2018Retractedu2019. ,