Long noncoding RNA CASC15 is upregulated in glioma and facilitates cell proliferation and metastasis via targeting miR-130b-3p
Authors: Y Xie
Y Cheng
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: lncRNA; CASC15; glioma; tumorigenesis; RT-qPCR
Affiliations: Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, .

Abstract:  Objective: Recent studies have discovered a class of long non-coding RNAs (lncRNAs) which are dysregulated in various tumors and linked to carcinogenesis. This paper aimed to uncover the molecular functions of lncRNA CASC15 in glioma tumorigenesis. Patients and methods: Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was performed to detect CASC15 expression in 50 glioma samples and 4 glioma cells. Besides, proliferation assay, transwell assay and wound healing assay were performed to explore the role of CASC15 in glioma progression in vitro. Furthermore, the interaction between CASC15 and miR-130b-3p in glioma was studied by performing the Dual-Luciferase reporter assay. In addition, tumor formation and metastasis assay were performed in vivo. Results: CASC15 expression was remarkably upregulated in glioma samples compared with that in adjacent samples. Cell proliferation, invasion and migration in glioma were inhibited via knockdown of CASC15 in vitro. Moreover, miR-130b-3p was upregulated via knockdown of CASC15 in vitro. Besides, miR-130b-3p was a direct target of CASC15 in glioma. Tumor formation and metastasis were inhibited after CASC15 was knockdown in vivo. Conclusions: These results suggest that CASC15 could repress metastasis and proliferation of glioma by sponging miR-130b-3p in vitro and in vivo, which may offer a new therapeutic intervention for glioma patients. 

Retracted: Yes

PubPeer comments:
 This paper is one of a set of 121 papers that all contain images from the same library of about 100 photos and plots. This is unexpected, because each paper has different authors and different affiliations, with little to no overlap, and describes different cancer types and molecules. It would be very hard to explain how authors from different labs would all obtain the same photo. Therefore, the papers might have all be derived from the same source, such as a paper mill that sells ready-to-be-published papers. This  Stock Photo paper set has been described in more detail on my blog post, https: scienceintegritydigest.com 2020 07 05 the-stock-photo-paper-mill and in a Wall Street Journal article by Eva Xiao (currently behind a paywall): https: www.wsj.com articles chinese-research-papers-raise-doubts-fueling-global-questions-about-scientific-integrity-11593939600 The vast majority (113 121) of the papers were published in the same journal, European Review for Medical and Pharmacological Sciences (ERMPS). A link to the full set of papers can be found here: https: docs.google.com spreadsheets d 172RWS18w1lTNJ9Nnj80fuXXTqTm4fGkoK0sdYyvKE3g edit?usp=sharing All papers have been reported to the journal editors on July 2, 2020. , 
 Some time in the last week or so, the journal wished the paper into the cornfield: https: www.europeanreview.org article 22240 Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cLong noncoding RNA CASC15 is upregulated in glioma and facilitates cell proliferation and metastasis via targeting miR-130b-3p, by Y. Xie, Y. Cheng, published in Eur Rev Med Pharmacol Sci 2019; 23 (17): 7475-7481u2013DOI: 10.26355 eurrev_201909_18857u2013PMID: 31539135u201d has been withdrawn. The Publisher apologizes for any inconvenience this may cause. ,