Angelica polysaccharide mitigates lipopolysaccharide-evoked inflammatory injury by regulating microRNA-10a in neuronal cell line HT22
Authors: Xiaoqian Guo
Yuni Zhou
Weimei Chen
Jun Liu
Journal: Artificial Cells Nanomedicine and Biotechnology (2019)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: Epilepsy; JAK2/STAT3; NF-κB; angelica polysaccharide; lipopolysaccharide
Affiliations: Department of Neurology, Jining Psychiatric Hospital, Jining, China;, Department of Neurology, Jining No.1 People’s Hospital, Jining, China;, Department of Neurosurgery, Jining No.1 People’s Hospital, Jining, China

Abstract:  Background: Angelica polysaccharide (AP) is disengaged from the roots of Angelica sinensis. The extensive pathological activities of AP have been discovered in disparate diseases. Nevertheless, the impression of AP in epilepsy (EP) remains unaware. The research attempted to probe the impact of AP on lipopolysaccharide (LPS)-evoked inflammatory injury in HT22 cells. Methods: AP were exploited to stimulate HT22 cells, cell cytotoxicity was monitored by CCK-8 assay. LPS was utilized to administrate HT22 cells to evoke inflammatory injury, meanwhile the involvements of AP in cell proliferation, apoptosis and inflammatory cytokines productions were examined. MicroRNA-10a (miR-10a) inhibitor and its negative control were one by one transfected into HT22 cells, the effect of miR-10a inhibition on LPS- and AP-treated cells was determined. NF-κB and JAK2/STAT3 pathways were ultimately detected. Results: AP promoted cell proliferation, inhibited apoptosis and suppressed IL-1β, TNF-α and IL-6 productions in LPS-stimulated HT22 cells. Additionally, AP raised miR-10a expression in HT22 cells administration with LPS. These functions of AP in LPS-disposed cells were conversed by miR-10a suppression. Further, AP interdicted NF-κB and JAK2/STAT3 pathways via enhancing miR-10a. Conclusions: Data corroboarted that AP mitigated LPS-evoked inflammatory injury through repression of NF-κB and JAK2/STAT3 pathways by regulating miR-10a in HT22 cells. 

Retracted: No

PubPeer comments:
 The Western blots and other figures in this paper have an interesting layout that appears to be shared with many other papers, as discussed in a January 2020 post on Leonid Schneider s blog: https: forbetterscience.com 2020 01 24 the-full-service-paper-mill-and-its-chinese-customers The list of these papers is available here: https: docs.google.com spreadsheets d 1KXqTAyl4j-jVorFPMD2XRpr76LcIKJ0CVyIvRj0exYQ edit?usp=sharing In almost all papers, Western blot panels within the same figure, and across figures and papers, appear to share the same background, while the bands are regularly spaced, all have similar rounded edges without the usual smudges and specks, and with some bands showing a recognizable  jumping sardine shape. Also in almost all papers, bar graphs show a characteristic layout with bars in solid shades of grey, and the left-most bar in black, as well as double-edged error bars. Many papers have  cell viability and  apoptotic cells graphs. To illustrate the recognizable layout of the graphs in this paper, here is Figure 1. ![file] , 
 Expression of Concern, [6 April 2021] . ------------------------------- We, the Publisher and Editor of Artificial Cells, Nanomedicine, and Biotechnology, are issuing an Expression of Concern for the following article: Yuni Zhou, Xiaoqian Guo, Weimei Chen Jun Liu (2019) Angelica polysaccharide mitigates lipopolysaccharide-evoked inflammatory injury by regulating microRNA-10a in neuronal cell line HT22. Artificial Cells, Nanomedicine, and Biotechnology, 47(1), 3194u20133201, DOI: 10.1080 21691401.2019.1614595 After publication of this article, questions about the scientific integrity of the article content were brought to the Publisher and Editoru2019s attention. We reached out to the authors requesting that they supply information that would confirm the articleu2019s integrity. The authors provided several files in response to our queries, but they were unable to provide the original data in a format which satisfies our requirements to confirm the authenticity of some types of data. In particular, they were unable to provide full western blots, where the molecular weight markers are indicated. Therefore, as we continue to work through the issues raised, we advise readers to interpret the information presented in the article with due caution. The authors have been notified about this Expression of Concern. , 
 [Source: Jining First People s Hospital Release time: 2021-12-17] : In order to strengthen the construction of scientific research integrity, severely punish violations, and create a good environment for scientific and technological innovation, the relevant paper issues recently investigated and dealt with by this unit are hereby notified as follows. 5. The paper  Angelica polysaccharide mitigates lipopolysaccharide-evoked inflammatory injury by regulating microRNA is published by Liu Jun of our hospital as the corresponding author, Zhou Yuni of Shandong Daizhuang Hospital (formerly Jining Psychiatric Center) as the first author, and Guo Xiaoqian as the co-first author. -10a in neuronal cell line HT22 , after investigation, it is an academic misconduct of tampering with data and fabricating the research process. Our hospital will deal with the relevant responsible persons as follows: Liu Jun (corresponding author): cancel the application qualifications for scientific research projects, scientific research awards, scientific and technological achievements, and scientific and technological talent plans within 5 years; cancel the nomination or recommender, nominee or recommender for 5 years , evaluation experts and other qualifications; cancel the membership or membership of academic institutions such as societies, associations, etc.; postpone promotion to a higher-level professional and technical position for one year; order to contact the magazine as soon as possible to withdraw the manuscript; warning from the party. Guo Xiaoqian (tied for the first author): A talk on scientific research integrity admonitions; criticism was circulated in the hospital. Chen Weimei (participating author): A talk on scientific research integrity admonitions; criticism was circulated in the hospital. Shandong Daizhuang Hospital (formerly Jining Psychiatric Center) dealt with Zhou Yuni (the first author) as follows: cancel the application qualifications for scientific research projects, scientific research awards, scientific and technological achievements, scientific and technological talent plans within 5 years; cancel the nomination or recommendation within 5 years Persons, nominees or recommenders, review experts and other qualifications, cancel their membership or membership in academic institutions such as societies and associations; postpone promotion to higher-level professional and technical positions for one year; circulate criticism and administrative warnings in the hospital. ,