MicroRNA-214 suppresses oncogenesis and exerts impact on prognosis by targeting PDRG1 in bladder cancer
Authors: Haiyan Wang
Jinfeng Wang
Xin Zhang
Lutao Du
Yongmei Yang
Zhaogang Dong
Chuanxin Wang
Lili Wang
Journal: PLoS ONE (2015)
Discipline: Multidisciplinary
Keywords: MicroRNA-214; bladder cancer; clinical correlation; biological significance; molecular network
Affiliations: Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan, Shandong Province, China; Department of Clinical Laboratory, Linyi People's Hospital, Linyi, Shandong Province, China., Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan, Shandong Province, China.

Abstract:  MicroRNA-214 (miR-214) has been reported to be dysregulated in human bladder cancer tissues. We aimed to investigate the clinical correlation, biological significance and molecular network of miR-214 in bladder cancer. Our results showed miR-214 was down-regulated in bladder cancer tissues and significantly associated with tumor stage, lymph node status, grade, multifocality, history of non-muscle-invasive bladder cancer (NMIBC). Moreover, miR-214 could serve as an independent factor of recurrence-free survival (RFS) and overall survival (OS) for patients with muscle-invasive bladder cancer (MIBC). Restoration of miR-214 expression in bladder cancer cell lines inhibited cell proliferation, migration, invasion and markedly promoted apoptosis. Dual-luciferase reporter assay recognized PDRG1 as direct downstream target gene of miR-214. PDRG1 was significantly increased in tumors low of miR-214 and knockdown of PDRG1 mimicked the effects of miR-214 overexpression. Our findings manifest that miR-214 could exert tumor-suppressive effects in bladder cancer by directly down-regulating oncogene PDRG1 and suggest an appealing novel indicator for prognostic and therapeutic intervention of bladder cancer. 

Retracted: Yes

PubPeer comments:
 there is a region of overlap between Figures 2B and 2C although these 2 sets of panels are supposed to represent different things ![file] , 
 there appears to be an inappropriate duplication of portions of differently labeled panels in Figure 3A ![file] , 
 some more similarities in different figures labeled as being different things ![file] , 
 #3 collected duplicated areas from comments 1 and 3 ![file] , 
 Retracted [17 December 2020] . --------------- Following the publication of this article [1], concerns were raised regarding the results presented in Figs 3 and 6. Specifically, - The 5637 miR-NC-0h panel of Fig 3A partially overlaps with the 5637 miR-214-0h panel of Fig 3A. - The 5637 miR-214-0h panel of Fig 3A partially overlaps with the 5637 si-NC-0h panel of Fig 6A. - The T24 si-NC-0h panel of Fig 6A partially overlaps with the T24 si-PDRG1-0h panel of Fig 6A. - Partial overlap has been detected between the T24-miR-214 panel of Fig 3B, the T24-miR-214 panel of Fig 3C, the T24-si-PDRG1 panel of Fig 6B and the T24-si-PDRG1 panel of Fig 6C. The authors stated that the similarity observed between the panels described above are the result of inadvertent errors introduced during figure preparation and provided data underlying their published results. However, the extent of the image concerns observed in this article raise serious concerns regarding the handling of the data obtained during this study and the integrity of the published results. In light of the concerns affecting multiple figure panels that question the integrity of these data, the PLOS ONE Editors retract this article. XZ agreed with the retraction. LW, YY, ZD, HW, and LD either did not respond directly or could not be reached. JW and CW did not agree with the retraction. ,