Long non-coding RNA UCA1 targets miR-185-5p and regulates cell mobility by affecting epithelial-mesenchymal transition in melanoma via Wnt/β-catenin signaling pathway
Authors: Yingli Pan
Juan Gao
Xige Chen
Zhengjuan Zhao
Yanhua Yu
Journal: Gene (2018)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Epithelial-mesenchymal transition; Melanoma; UCA1; Wnt/β-catenin; miR-185-5p.
Affiliations: Department of dermatology, Weihai Central Hospital, Shandong, China., Department of dermatology, Weihai Central Hospital, Shandong, China. Electronic address: .

Abstract:  This article has been retracted: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). This article has been retracted at the request of the Editor-in-Chief. Several figures presented in the manuscript appear to have been doctored. The journal has tried to contact the authors of this article but at the time of publication of this notice has not received any response. As there is no explanation for the picture manipulations, the Editor-in-Chief of Gene has lost confidence in the validity of this work and has decided to retract it. 

Retracted: Yes

PubPeer comments:
 Fig 5.  (B) Western blot analysis of Wnt5a protein expression in human OS tissue samples and their corresponding normal tissues. GAPDH was used as an internal control. (C) **qRT-PCR analysis** of Wnt5a expression in four OS cell lines (HOS, Saos-2, U2OS and MG-63) and normal osteoblast cells (NHOst). ![file] It is difficult to understand how the authors or reviewers could have mistaken 5C for qRT-PCR results. Both loading controls are versatile, appearing in a list of publications from multiple teams of authors - some cases are [described here.] For instance, Figs 4C, 6D of [Wen et al (2015)] . ![] ![] , 
 Fig 6B.  Wnt5a ... protein (B) levels were detected in U2OS cells 48 h after transfection with pcDNA3.0-Wnt5a or the control vector by ... western blot analysis . ![file] The loading control here is another frequent-flier, with other appearances including *[above]* Fig 5D from [Liu et al (2016)] .  The ADAM10 protein expression was measured in four HCC cell lines (SMMC-7721, Hep3B, HepG2, Huh-7) and normal hepatic cell line HL-7702 by western blot . [below] Fig 4D of [Dong et al (2016)] . ![] See other examples linked in those threads. The substantive band also appears to be a composite. Other manifestations include ADAM-9 from Fig 1C of [Chang et al (2016)] and ADAM-10 from Fig 5D of Liu et al (2016). ![] Earlier, as Survivin in Fig 5 of [Pan et al (2015)] . ![] , 
 Fig 2D from [**Zhou et al (2016)**] .  Cell cycle distribution of U2OS cells transfected with the miR-154 mimic was determined by flow cytometry . ![file] Clockwise from top left: - Fig 3A from [Liu et al (2015)] .  Effects of miR-222 inhibitor on HepG2 cell cycle distribution. (A) Cell DNA content distribution in each phase. - Fig 3A from [Zhang et al (2015)] .  Effects of microRNA-145 (miR-145) on multiple myeloma (MM) cell cycle distribution. (A) Cell DNA content distribution in each phase. - Fig 3A from [Li et al (2015)] .  Enforced expression of miR-338-3p induced cell cycle distribution and cell apoptosis in EC9706 cells. (A) Cell DNA content distribution in each phase. - Fig 3A from [Zhang et al (2015b)] ,  miR-137 induces changes in cell cycle distribution and cell apoptosis in SKOV3 cells. (A) Cell DNA content distribution in each phase ![file] The same panels keep appearing. *[left]* Fig 2A from [Liu et al (2015)] .  Effect of DOX and Ad sh-NOB1 alone or in combination on cell cycle of TPC-1 cells. (A) Cell DNA content distribution in each phase . *[upper right]* Fig 2C from [Song et al (2015)] .  Determination of HeLa cell cycle distribution by PI staining after transfection with miR-133a or miR-Ctrl . *[lower right]* Fig 2d from [Zhang et al (2015)] .  The cell cycle was detected by PI staining after being transfected with miR-133a or miR-NC. ![file] The same panels keep appearing. Clockwise from upper left: - Fig 2D from [Pang et al (2015)] .  cell cycle progression ... determined in HepG2 cells transfected with the miR-154 mimic or miR-NC. - Fig 2D from [Zhao et al (2017)] .  cell cycle stage ... assessed in MM1S cells transfected with miR-144-3p mimic or miR-NC. - Fig 2D from [Li et al (2016)] .  The cell cycle procession was detected by flow cytometry after transfection with miR-494 or miR-NC . - Fig 2C from [Luan et al (2015)] .  Cell cycle was measured in glioma cells at posttransfection by flow cytometry . ![file] The same panels keep appearing. , 
 Retracted [July 5 2021] . ---------------------------------- Following the publication of this paper, it was drawn to the Editors attention by a concerned reader that the western blotting data featured in Fig. 5B and C, and also in Fig. 6B, were strikingly similar to data appearing in different form in other articles by different authors at different research institutes. Owing to the fact that the contentious data in the above article were already under consideration for publication, or had already been published, elsewhere prior to its submission to *Oncology Reports*, the Editor has decided that this paper should be retracted from the Journal. After having been in contact with the authors, they agreed with the decision to retract the paper. The Editor apologizes to the readership for any inconvenience caused. ,