Screening differential circular RNA expression profiles reveals the regulatory role of circTCF25-miR-103a-3p/miR-107-CDK6 pathway in bladder carcinoma
Authors: Mengxin Lv
Junxia Chen
Zhenyu Zhong
Journal: Scientific reports (2016)
Discipline: Multidisciplinary
Keywords: circRNAs; gene regulation; miRNA sponge effects; microRNA response elements; bladder carcinoma
Affiliations: The First Clinical College, Chongqing Medical University, Chongqing 400016, China., Department of Cell Biology and Genetics, Chongqing Medical University, Chongqing 400016, China.

Abstract:  Circular RNAs (circRNAs), a kind of non-coding RNAs, have shown large capabilities in gene regulation. However, the mechanisms underlying circRNAs remain largely unknown so far. Recent studies demonstrated that circRNAs play miRNA sponge effects and regulate gene expression by microRNA response elements. Here, we screened circRNA expression profiles of bladder carcinoma using microarray assay. A total of 469 dysregulated circular transcripts are found in bladder cancer compared with normal tissues, among which 285 were up-regulated and 184 were down-regulated. Six circRNAs were identified to have significant differences by qRT-PCR. We speculated that circRNAs might involve in cancer-related pathways via interactions with miRNA by multiple bioinformatical approaches. Therefore, we further predicted that circTCF25 could sequester miR-103a-3p/miR-107, which potentially lead to the up-regulation of thirteen targets related to cell proliferation, migration and invasion. Subsequently, we demonstrated that over-expression of circTCF25 could down-regulate miR-103a-3p and miR-107, increase CDK6 expression, and promote proliferation and migration in vitro and vivo. This is the first study to exploit circRNA profiling and circRNA/miRNA interactions in bladder cancer. Our work laid the foundation to investigate the functions of circRNAs in cancers. The data also suggest that circTCF25 might be a new promising marker for bladder cancer. 

Retracted: No

PubPeer comments:
 This article shares the same data and conclusion with a chinese paper (DOI: 10.11844 cjcb.2018.06.0354) which is also an article of Chen s. ![file] , 
 We published English article in 2016 years and Chinese paper in 2018 years. We only used one picture in English to split it into several small panels in Chinese journal and replenished additional experiment. Before, magazine editors said that publishing in different languages was not called repeated publishing, of course, I will not. Thank you for your attention and reminder. , 
 #2 Dear Dr. Chen, Thanks for your effort to bring more research data to China. However, I doubt if the Chinease version received the copyright from scientific reports. Moreover, 15 figures in the Chinease version but only 2 were different from the English one. Also the 15 firgues were not from one pic in English. And you also not mention your first publication in the references of Chinese version, which does not comply with knowledge dissemination requirements. , 
 This article shares the same data and conclusion with a chinese paper (DOI: 10.11844 cjcb.2018.06.0354) which is also an article of Chen s, however, except the corresponse author, the first and the second author are totally different. The figure 5A and figure7 of this english article are totally identical with the figure1, figure2, figure3, figure4, figure5, figure6A, figure7, figure8 of the chinese paper (DOI: 10.11844 cjcb.2018.06.0354) . Most importantly, these two articles share the same data and same conclusion. The chinese article is the part of this english article. The figure 5 and 7 of english article are identical with the figure1, figure2, figure3, figure4, figure5, figure6A, figure7, figure8 of the chinese paper (DOI: 10.11844 cjcb.2018.06.0354) . ![file] ![file] ![file] ,