Telomerase reconstitution contributes to resetting of circadian rhythm in fibroblasts
Authors: Zhengrong Wang
Jianmin Ding
Lin Zhang
Zhou Jiang
Yi Qu
Chaomin Wan
Yanyou Liu
Meng Mao
Xihong Li
Dezhi Mu
Journal: Molecular and Cellular Biochemistry (2008)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: circadian rhythms; aging; cell senescence; telomerase reconstitution; rhythmic gene expression
Affiliations: Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China.

Abstract:  The synchronization of the circadian signals to external or suprachiasmatic nucleus stimulation in the peripheral clocks is essential for maintaining the usual function of human body. However, aging will disrupt the synchronization of peripheral circadian rhythms, thus leading to some age-associated diseases. Up to now, little is known about the modification of the oscillatory rhythms in aged cells. A recent report showed that cell senescence in vascular human smooth muscle cells (HSMCs) altered circadian rhythms by a dysregulation of rhythmic gene expression. Furthermore, this alteration could be reversed by telomerase reconstitution. To test whether telomerase reconstitution can restore disrupted circadian rhythm in other types of senescent cells, we used fibroblasts as cell models to profoundly investigate the relationship between cell senescence and circadian rhythm modulation. We found that the response of rhythmic gene expression to serum stimulation was markedly attenuated in senescent fibroblasts, telomerase-reconstituted fibroblasts reset the circadian oscillation of rhythmic gene expression, and the activation of pERK-CREB and p38-CREB pathways might be involved in the circadian rhythm resetting. These findings suggested that telomerase reconstitution might be a good way to reset synchronization of peripheral circadian rhythms disrupted in senescent tissues. 

Retracted: No

PubPeer comments:
 Figure 4B. Could the authors please check? * Red lines: Four lanes in the Pi-ERK panel look very similar to each other. * Blue lines: Five lanes in the Pi-CREB panel look very similar to each other. ![file] , 
 Dear Dr. Bik, Thank you very much for pointing out the errors of the figurer. I have contacted the first author to carefully check the figures since the figures were provied from the first author in this paper and will response to you as soon as possible. Sincerely, Dezhi Mu , 
 Dear Dr. Bik, Iu2019m the first author of this paper. I have discussed about this picture with other authors and we regarded that these lanes are similar but not the same. , 
 #3 Dr. Yi Qu: Thank you for replying. Unfortunately after examining this image, I agree with Dr. Biku2019s concerns. Would you be able to provide original images to address the concern that Figure 4B does not accurately represent the experimental results? Separately, I would note that this journal is a member of the Committee on Publication Ethics (COPE). *** Inverted view of 4B ![file] , 
 Correction, June 2020: https: link.springer.com article 10.1007 s11010-020-03785-9  In the original article, Fig. 4b was published incorrectly in which four to five lanes in Pi-ERK and Pi-CREB panels look very similar to each other (Telomerase reconstitution contributes to resetting of circadian rhythm in fibroblasts, Mol Cell Biochem, 2008, 313:11u201318). Since this image was stored in The Experiment Center of the West China Second University Hospital, Sichuan University, which was dissoluted in 2012, the original data cannot be traced. Experiments were therefore redone to verify the result and the correct version of Fig. 4b is provided in this correction. ,