Alpinumisoflavone induces apoptosis in esophageal squamous cell carcinoma by modulating miR-370/PIM1 signaling
Authors: Jianjun Peng
Ning Zhao
Hui Gao
Xiuwei Yang
Xia Qiu
Yantao Han
Journal: American journal of cancer research (2016)
Discipline: CLINICAL MEDICINE
Keywords: ESCC; PIM1; alpinumisoflavone; miR-370.
Affiliations: Qingdao University Qingdao 266071, Shandong, China., Qingdao University Affiliated Hospital Qingdao 266071, Shandong, China., College of Life Sciences, Chongqing Normal University Chongqing 401331, China., 92196 Military Hospital Qingdao 266011, Shandong, China.

Abstract:  Esophageal squamous cell carcinoma (ESCC) is the most prevalent type of esophageal cancer and accumulating evidence has confirmed the role of miRNAs in ESCC. One such miRNA, miR-370, was found to be aberrantly downregulated in various human malignancies. This study showed that the expression of miR-370 was significantly lower in ESCC tissues and cell lines, and miR-370 functioned as a tumor suppressor in ESCC. Moreover, this is the first report that showed miR-370 suppresses cell proliferation and tumor growth by directly targeting Pim family kinases 1 (PIM1). Furthermore, alpinumisoflavone, a naturally occurring flavonoid, could inhibit tumor growth of ESCC by targeting miR-370/PIM1 signaling. 

Retracted: No

PubPeer comments:
 Fig 5D ( Overexpression of PIM1 in cells transfected with miR-370 mimic leads to apoptosis inhibition ) at right. One might wonder why one of the FACS plots later appeared in (at left) Fig 6B of [Li et al (2017)] ( NAC or Compound C significantly attenuates the apoptosis-inducing effect of PG in both GBM cell lines ) with a different gated value, derived from a different cell line and a different phytochemical. ![file] , 
 Fig 4D would be more convincing if the upper-left and upper-right FACS plots differed in more than just the gate percentages. ![file] , 
 Figures 6C, 7F. These are the earliest sightings to date of some images that regular regularly through this oeuvre of papers. ![file] Compare, for instance, 3B from [Liu Yu (2018)] : ![] , 
 At right, Fig 4E:  PIM1 shRNA impairs the pro-growth effect of miR-370 inhibitor in ESCC cells. ... E. Knockdown of PIM1 in cells transfected with miR-370 inhibitor results in caspase-3 activation. Unexpected similarities with (at left) Figure 4A from [Chen et al (2020)] :  AF induces ferroptosis though breaking iron homeostasis. A. The levels of ferroptosis-related protein TRF, FTH, FPN were analyzed by western blotting after treatment with increasing concentration of AF (0, 10, 20 u03bcM). ![file] , 
 Below, Fig 2D  MiR-370 functions as a tumor suppressor in ESCC cells. ... D. Mi-370 mimic suppresses cell proliferation, while miR-370 inhibitor promotes cell growth. One colony plate (red) seems to be repeated, after a 90-deg rotation and cropping (pink). It, and the plate marked with blue, reappear in (above) Fig 1D of [Lin et al (2019)] :  Following incubation with FL118 (0, 10, and 20 nM) for 14 days, the numbers of colonies of HCT-116 and SW480 cells were quantified using colony formation assay ![file] , 
 Middle left, Fig 7E:  MiR-370 inhibitor or PIM1 overexpression significantly abrogates the anti-growth effect of AIF . Middle right, 1C from [Xue et al (2019)] ( The effect of PG on colony formation was assessed ). Above, from [Wang et al (2017)] : Figs 1B ( PG suppresses cell proliferation, as determined by colony formation assay ) and 3C ( Ectopic overexpression of PIM1 partly abrogated the anti-proliferative effect of PG both HCC cell lines, as determined by colony formation assay ). Below: Fig 1C from [Ma et al (2019)] :  Fur A inhibits growth of HT29 and SW480 cells. ... C. Fur A inhibited anchorage-independent growth of CRC cells. ![file] Numerous colony slides in common, differently identified. No authors in common. , 
 http: www.most.gov.cn zxgz kycxjs kycxgzdt 202201 t20220127_179206.html Google Translated Date: January 27, 2022 14:44 Source: Ministry of Science and Technology u3000u300018. The paper  Alpinumisoflavone induces apoptosis in esophageal squamous cell carcinoma by modulating miR-370 PIM1 signaling by Gao Hui from the School of Pharmacy of Qingdao University as the corresponding author and Han Yantao from the School of Basic Medicine as the first author. Use behavior; Han Yantao is the corresponding author and Gao Hui is the first author of the paper  Hispidulin suppresses tumor growth and metastasis in renal cell carcinoma by modulating ceramide-sphingosine 1-phosphate rheostat . After investigation, the paper has inappropriate signatures and data reuse behavior. Han Yantao was unaware that the paper was signed. Gao Hui (merged with the other 4 papers) will be dealt with as follows: demerit for 1 year, cancellation of application or application for science and technology projects (special projects, funds, etc.), science and technology awards, and qualifications for science and technology talent titles for 9 years, cancellation as a nomination or recommendation Persons, nominees or recommenders, review experts, etc. have been qualified for 9 years, recorded in the file of scientific research integrity and dishonesty, canceled from participating in this year s evaluation, and the annual evaluation shall not be determined as qualified or above. u3000u3000(Notification link: https: kjc.qdu.edu.cn info 1023 3178.htm) ,