Controlled release of drug and better bioavailability using poly(lactic acid-co-glycolic acid) nanoparticles
Authors: Pralay Maiti
Sanjeev K Pandey
Manjula Vinayak
Akhilendra K Maurya
Durga P Mishra
Chandana Haldar
Dinesh K Patel
Ravi Thakur
Journal: International Journal of Biological Macromolecules (2016)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: Controlled release; Emulsified nanoprecipitation; Nanoparticles; PLGA; Tamoxifen.
Affiliations: Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221 005, India. Electronic address: ., School of Materials Science and Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi 221 005, India., Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221 005, India., Cell Death Research Laboratory, Division of Endocrinology, Central Drug Research Institute, Lucknow 226031, India., School of Materials Science and Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi 221 005, India. Electronic address: .

Abstract:  Tamoxifen (Tmx) embedded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (PLGA-Tmx) is prepared to evaluate its better DNA cleavage potential, cytotoxicity using Dalton's lymphoma ascite (DLA) cells and MDA-MB231 breast cancer cells. PLGA-Tmx nanoparticles are prepared through emulsified nanoprecipitation technique with varying dimension of 17-30nm by changing the concentrations of polymer, emulsifier and drug. Nanoparticles dimension are measured through electron and atomic force microscopy. Interactions between tamoxifen and PLGA are verified through spectroscopic and calorimetric methods. PLGA-Tmx shows excellent DNA cleavage potential as compared to pure Tmx raising better bioavailability. In vitro cytotoxicity studies indicate that PLGA-Tmx reduces DLA cells viability up to ∼38% against ∼15% in pure Tmx. Hoechst stain is used to detect apoptotic DLA cells through fluorescence imaging of nuclear fragmentation and condensation exhibiting significant increase of apoptosis (70%) in PLGA-Tmx vis-à-vis pure drug (58%). Enhanced DNA cleavage potential, nuclear fragmentation and condensation in apoptotic cells confirm greater bioavailability of PLGA-Tmx as compared to pure Tmx in terms of receptor mediated endocytosis. Hence, the sustained release kinetics of PLGA-Tmx nanoparticles shows much better anticancer efficacy through enhanced DNA cleavage potential and nuclear fragmentation and, thereby, reveal a novel vehicle for the treatment of cancer. 

Retracted: No

PubPeer comments:
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 July 2018 Corrigendum found here: https: www.sciencedirect.com science article pii S0141813018311310  The authors regretfully submit that there was human mistake and it can be rectified by adding a corrigendum in which the following be mentioned: [image] Fig. 1a incorrect one be immediately replaced by the Fig. 1a correct one. The human mistake was due to folders having electron micrograph of all experiment and during transfer of images from technical person to us. The authors would like to apologies for inconvenience caused. ,