MicroRNA-122 inhibits proliferation and invasion in gastric cancer by targeting CREB1
Authors: Li Feng
Yonggang Zhu
Xiaoxia Cong
Min Rao
Yinan Zhou
Journal: American journal of cancer research (2017)
Discipline: CLINICAL MEDICINE
Keywords: CREB1; gastric cancer; invasion; miR-122; proliferation.
Affiliations: Department of Gastroenterology, The People's Hospital of Jilin Province Changchun 130021, China., Department of Radiotherapy, China-Japan Union Hospital of Jilin University Changchun 130033, China., Department of Gastrointestinal Surgery, The People's Hospital of Jilin Province Changchun 130021, China.

Abstract:  MicroRNA-122 (miR-122) has been implicated in tumor development and progression in various types of cancers. However, the biological function and regulatory mechanisms of miR-122 in gastric cancer (GC) remain largely unknown. We aimed to determine the biological role and underlying mechanism of miR-122 in GC. Real time quantitative RT-PCR (qRT-PCR) was performed to detect the expression of miR-122 in GC tissues and cell lines. CCK8, wound healing, and transwell assays were conducted to determine the effect of miR-122 on cell proliferation, migration, and invasion, respectively. Target molecules were identified by luciferase activity, quantitative RT-PCR, and western blotting. We found that miR-122 expression was significantly decreased in both GC tissues and cell lines and that reduced expression was significantly associated with aggressive clinicopathological features in patients. We also found that overexpression of miR-122 markedly inhibited proliferation, migration, and invasion in GC cell lines. In addition, cAMP responsive element binding protein 1 (CREB1) was identified as a direct target of miR-122, and its expression was negatively correlated with miR-122 expression in GC tissues (r = -0.711, P &lt; 0.001). CREB1overexpression rescued the suppressive effect of miR-122 on GC cell proliferation, migration, and invasion. Moreover, we demonstrated that miR-122 inhibited GC tumorigenesis in vivo by repressing CREB1 expression. These findings suggest that miR-122 might function as a tumor suppressor in GC and could serve as a promising candidate for therapeutic applications regarding GC treatment. 

Retracted: Yes

PubPeer comments:
 Fig 6B.  **miR-122** suppresses xenograft tumor growth in vivo by targeting CREB1. ... B. The mice were killed at 30 days after inoculation, and **[SGC-7901]** tumor tissues was excised and imaged. ![file] Fig 6 from [Zhang et al (2016)] .  **miR-22** suppresses tumor growth in a **768-O** xenograft model. (A) Photographs of tumor tissue collected after sacrifice at day 30 . ![file] Fig 4A from [Ge et al (2016)] .  **miR-497** inhibits tumor growth in vivo. (A) Representative image of tumors formed in BALB c xenograft mouse models at 28 days following injection with human osteosarcoma **MG63** cells transfected with a miR-497 mimic or miR-control. ![file] , 
 Dear pubpeeruff1b Thank you for your comment.We will examine the orignal date and get back to you soon. , 
 #2 Fig 6B again: ![file] If it s any assistance, the first sighting was as Fig 5A of [Li et al (2015)] .  Representative images of the tumors in the different treatment group . ![file] , 
 This article was retracted. Not reproducible, and  original data was missed due to irresistible cause . No DOI but can be found on PMC @ [PMC8263659]  In this article, we are not able to reproduce in vivo experiment. Moreover, some original data was missed due to irresistible cause. Therefore, we want to retract this paper in Am J Cancer Res. We sincerely apologize for any inconvenience that might result from the retraction of this article. ,