LncRNA HANR aggravates the malignant progression of glioma via targeting miRNA-335
Authors: D Liu
K Sun
X-D Wang
J Liu
W-J Wang
F-Y Li
X-B Hui
Journal: European review for medical and pharmacological sciences (2020)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: lncRNA HANR; glioma progression; metastasis; prognosis; miRNA-335
Affiliations: Department of Neurosurgery, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai'an, China. .

Abstract:  Objective: The aim of this study was to uncover the role of lncRNA HANR in the progression of glioma and the underlying mechanism. Patients and methods: HANR expression level in 36 matched glioma tissues and adjacent non-tumoral tissues was determined by qRT-PCR. The relationship between HANR expression and pathological indexes of the glioma patients was analyzed. The Kaplan-Meier method was introduced to investigate the survival of glioma patients. After the knockdown of HANR, the proliferative, migratory, and invasive changes of U251 and SHG44 cells were determined. Bioinformatics and Dual-Luciferase Reporter Gene Assay were applied to predict and verify the downstream target of HANR, respectively. Furthermore, the rescue experiments were conducted to clarify the role of HANR/miRNA-335 regulatory loop in the progression of glioma. Results: HANR was significantly upregulated in glioma tissues and cell lines. Glioma patients with a high expression level of HANR presented remarkably higher rates of lymphatic metastasis and distant metastasis, as well as worse prognosis. The silence of HANR remarkably attenuated the proliferative, migratory, and invasive capacities of U251 and SHG44 cells. MiRNA-335 was the direct target of HANR and was significantly downregulated in glioma tissues. Meanwhile, the miRNA-335 level was negatively regulated by HANR. In addition, the knockdown of miRNA-335 partially reversed the regulatory effects of HANR on cellular behaviors of glioma. Conclusions: LncRNA HANR is upregulated in glioma, which is closely correlated with metastasis and poor prognosis of glioma patients. In addition, HANR aggravates the progression of glioma by negatively regulating miRNA-335. 

Retracted: No

PubPeer comments:
 Figure 4C: * Pink boxes: Two panels appear to overlap with each other, except for a rogue letter  D . * Green boxes: One panel appears to overlap with a panel from Figure 2D of a different paper, i.e. L Yang et al., ERMPS (2020), DOI: 10.26355 eurrev_202002_20156 - https: pubpeer.com publications B324F0C5CC90125D4A9AA9F883A5DC In one paper, the photo with the green box represents glioma cells, in the other paper it represents ovarian cancer cells. Could the authors please explain how this could have happened? ![file] , 
 Figure 4C: * Yellow boxes: A panel in this figure appears to overlap with a panel in a paper by different authors, where it represents lymphoma cells. That paper is: CC Wang et al., doi: 10.26355 eurrev_202004_21022 - https: pubpeer.com publications 67A6DA90BC3875837836A82121A0EA ![file] , 
 Figure 2C. * Cyan boxes: A panel in this figure appears to overlap with a panel in Figure 2D of Y Li et al., ERMPS (2020), DOI: 10.26355 eurrev_202002_20169 - https: pubpeer.com publications FF65094B1FF240E784366446E88AE2, where it represents a very different experiment. Can the authors please explain how two very different research groups can publish a photo of the same specimen? ![file] , 
 Figure 2C. * Blue boxes: A panel in this figure appears to overlap with a panel in Figure 4E of DZ Fang et al., ERMPS (2020), DOI: 10.26355 eurrev_202001_19918 - https: pubpeer.com publications 2AFA82AD6FFBD11352FC668F913BDB, where it represents a different experiment. Can the authors please explain how two very different research groups can publish a photo of the same specimen? ![file] , 
 *[right]* Fig 3F from [Wang et al (2020b)] .  A negative correlation was observed between the expression levels of HANR and miR-335 in glioma tissues . *[left]* Fig 3E from [Wang et al (2020a)] .  A negative correlation between peripheral blood levels of FOXO1 and miRNA-584 in NK T-cell lymphoma patients . ![file] Implausibly many points in common. For comparison:  Here is a small collage of the plots (h t [Elisabeth Bik] ): ![] Here, an overlay of 20 correlation scatterplots from other papers (all based on the same spreadsheet), emphasising the points frequently occurring between them. ![file] , 
 Dear Sir, Thank you for your comments.There are some errors in my article.I will check the original data and contact the editor of European Review for Medical and Pharmacological sciences to correct our data.Thank you very much. ,