Retracted : Evaluation of the circulating MicroRNA‐495 and Stat3 as prognostic and predictive biomarkers for lower extremity deep venous thrombosis
Authors: La-Mei Yu
Nai-Xuan Li
Jing-Wu Sun
Journal: Journal of Cellular Biochemistry (2018)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: MicroRNA-495; Stat3; lower limb fracture; perioperative deep vein thrombosis; peripheral blood
Affiliations: Department of Interventional Medicine, The Affiliated Hospital of Binzhou Medical University, Binzhou, P.R. China., Department of Vasculocardiology, The Affiliated Hospital of Binzhou Medical University, Binzhou, P.R. China., Department of Physiology, Binzhou Medical University, Yantai, P.R. China.

Abstract:  This study aims to elucidate the prognostic and predictive biomarker of miR-495 and Stat3 in peripheral blood in relation to lower extremity deep venous thrombosis (DVT). Patients with lower limb fractures were assigned into case and control groups. Rats were allocated into blank (normal rats), sham (normal rats), DVT, miR-495 mimic, miR-495 inhibitor, over-Stat3, and si-Stat3 groups. ELISA was used to detect levels of prothrombin time (PT), endothelin-1 (ET-1), Human Fibrinogen (FIB), D-Dimer, blood coagulation factors V and VIII, tissue type plasminogen activator (t-PA), platelet activating factor (PAF), protein C and Stat3. qRT-PCR was employed for the evaluation of the expressions of miR-495 and Stat3, while receiver operating characteristic (ROC) curve was constructed to assess the predictive value of miR-495 and Stat3 as well as the treatment outcomes of patients with lower limb fractures. Logistic regression analyses were conducted in order to correlate indexes and lower extremity DVT. miR-495 overexpression, t-PA, PAF, and protein C were confirmed to be protective factors, while Stat3 overexpression, PT, ET-1, FIB, D-Dimer, blood coagulation factor V, and VIII were all ultimately considered to be risk factors of lower extremity DVT. Stat3 was confirmed to be the target gene of miR-495. Compared with the blank group, the length and weight of the thrombus as well as the ratio between length and weight, mRNA and protein expression of Stat3 were reduced in the miR-495 mimic and si-Stat3 groups. Our findings suggest that through the suppression of Stat3 expression, miR-495 prohibits lower extremity DVT in peripheral blood. 

Retracted: Yes

PubPeer comments:
 Fig 6.  Conditions of inferior DVT formation in each group detected by HE staining . ![file] Two panels appear to be details of larger images, which appeared later as Fig 1 of [Bao et al (2018)] . ![file] , 
 Retracted [30 June 2021] . ---------------------------------- The above article, published online on 21 December 2017 in Wiley Online Library has been retracted by agreement between the journal s Editor in Chief, Prof. Dr. Christian Behl, and Wiley Periodicals LLC. The retraction has been agreed following an investigation based on allegations raised by a third party. A detailed investigation revealed that several image elements of the experimental data were published elsewhere in a different scientific context. Thus, the editors consider the conclusions of this article to be invalid. ,