MicroRNA-379 inhibits the proliferation, migration and invasion of human osteosarcoma cells by targetting EIF4G2
Authors: Xi Xie
Hong-Bo He
Yu-Sheng Li
Wei Luo
Zhen-Han Deng
Qing Liu
Wen-Feng Xiao
Journal: Bioscience Reports (2017)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: EIF4G2; Invasion; MicroRNA-379; Migration; Osteosarcoma
Affiliations: Department of Orthopedics, Xiangya Hospital, Central South University, Changsha 410008, P.R. China., Department of Day Surgery Center, Xiangya Hospital, Central South University, Changsha 410008, P.R. China., Department of Orthopedics, Xiangya Hospital, Central South University, Changsha 410008, P.R. China  .

Abstract:  Osteosarcoma (OS) is an aggressive malignant mesenchymal neoplasm amongst adolescents. The aim of the present study was to explore the various modes of action that miR-379 has on the proliferation, migration, and invasion of human OS cells. miR-379 achieves this by targetting eukaryotic initiation factor 4GII (EIF4G2). Human OS cell lines U2OS and MG-63 were selected and assigned into blank, miR-379 mimics, miR-379 mimic negative control (NC), miR-379 inhibitors, miR-379 inhibitor NC, EIF4G2 shRNA, control shRNA, and miR-379 inhibitor + EIF4G2 shRNA group. The miR-379 expression and EIF4G2 mRNA expression were detected utilising quantitative real-time PCR (qRT-PCR) and the EIF4G2 protein expression using Western blotting. MTT assay, scratch test, Transwell assay, and flow cytometry were performed to determine the proliferation, migration, invasion, and cell cycle, respectively. In comparison with the miR-379 mimic NC group, the miR-379 mimics group had decreased EIF4G2 expression; the miR-379 inhibitors group indicated an increased EIF4G2 expression. Compared with the control shRNA group, the EIF4G2 expression was lower in the EIF4G2 shRNA group and the miR-379 expression was dropped in the miR-379 inhibitor + EIF4G2 shRNA group. The proliferation, migration, and invasion abilities of OS cells were reduced in the miR-379 mimics and EIF4G2 shRNA groups. The percentage of OS cells at the G0/G1 stage was increased, and the percentage at the S-stage was decreased in the miR-379 mimics and EIF4G2 shRNA groups. miR-379 may inhibit the proliferation, migration and invasion of OS cells through the down-regulation of EIF4G2. 

Retracted: No

PubPeer comments:
 The authors of the original article u201cMicroRNA-379 inhibits the proliferation, migration and invasion of human osteosarcoma cells by targeting EIF4G2u201d (Bioscience Reports (2017) 37, DOI: 10.1042 BSR20160542) would like to correct Figure 5, as they had mistakenly used images of from another group in place of the blank group in Figure 5F and H due to nonstandard naming of the images. The authors express their sincere apologies for any inconvenience that this error has caused to the readers. The corrected version of Figure 5 is presented here. ![file] https: portlandpress.com bioscirep article 40 7 BSR-20160542_COR 225676 Correction-MicroRNA-379-inhibits-the-proliferation , 
 Fig 5B at lower right here. At left, Figs 8 and 9 from [Qiao et al (2017)] ; and upper right, Fig 5B from [Ba et al (2017)] . Mainly repeating Dendrodoa Grossularia s observations. I ran out of colors before I could include that commenter s observations on the links **within** Figs 8 and 9 of Qiao et al. ![] , 
 Fig 5D *[below]*. *[above]* Fig 4B from [Chen et al (2016)] . ![file] , 
 Fig 5B: ![file] One panel appears to match a (rotated) panel from Fig 6(a) of [Zhang et al (2019)] . ![file] , 
 #4 A panel was duplicated between Figs 5B and 5D. The duplication was removed in the Correction, but without acknowledgement that it had occurred. ![file] The miR-379 Inhibitors panel was duplicated between 5F and 5H. Again, the duplication was cleared up in the Correction without acknowledgement of its occurrence. ![file] Other color boxes here indicate images that also appeared in Fig 7A of [Xiong et al (2018)] . ![] UPDATE: Fig 7A of Xiong et al (2018) also overlaps with Fig 5 of [Liu et al (2017)] - here at upper left - allowing the latter to overlap with the present 5F H. ![] , 
 https: doi.org 10.1042 BSR-20160542_EOC The Editorial Office has been made aware of potential issues surrounding the scientific validity of this paper, hence has issued an expression of concern to notify readers whilst the Editorial Office investigates. , 
 New comparing the present Fig 5B... ![file] ...with Figs 6 and 11 from [Li et al (2015)] . ![file] H t to Dendrodoa Grossularia. , 
 Retracted [16 April 2021](10.1042 BSR-20160542_RET). --------------------------------------- This Retraction follows an Expression of Concern and Correction relating to this article previously published by Portland Press. This article is being retracted from Bioscience Reports at the request of the Editor-in-Chief and the Editorial Board following receipt of a notification from several readers, alerting the Editorial Board to multiple image duplications in the original versions of Figures 5B, 5D, 5F and 5H. The authors were contacted regarding the concerns raised and provided new replacement Figures; however, given that there were extensive duplications within the original and corrected images, the Editorial Board stands by the decision to retract the article. The authors agree to the retraction. ,