Effects of LncRNA-HOST2 on cell proliferation, migration, invasion and apoptosis of human hepatocellular carcinoma cell line SMMC-7721
Authors: Chang-Qing Yan
Run-Tian Liu
Yang Wang
Cong-Jing An
Jing-Lin Cao
Hai-Tao Lv
Journal: Bioscience reports (2017)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Hepatocellular carcinoma; Human ovarian cancer specific transcript 2; Long noncoding RNA; Migration; Proliferation
Affiliations: Department of Hepatobiliary Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050051, P.R. China., Department of Hepatobiliary Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang 050051, P.R. China ., Department of Hepatobiliary Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang 050051, P.R. China., Medical Examination Center, The Second Hospital of Hebei Medical University, Shijiazhuang 050051, P.R. China.

Abstract:  The present study explored the effect of long non-coding RNA-human ovarian cancer-specific transcript 2 (LncRNA-HOST2) on cell proliferation, migration, invasion and apoptosis of human hepatocellular carcinoma (HCC) cell line SMMC-7721. HCC tissues and adjacent normal tissues from 162 HCC patients were collected. The HCC cell lines were assigned into the control group (regular culture), negative control (NC) group (transfected with siRNA) and experimental group (transfected with Lnc-HOST2 siRNA). Quantitative real-time PCR (qRT-PCR) was used to detect the expression of LncRNA-HOST2. Cell proliferation was detected by CCK-8 and colony-forming assays, cell apoptosis by flow cytometry and cell migration by Scratch test. Transwell assay was used to evaluate cell migration and invasion abilities. LncRNA-HOST2 expression in the HCC tissues increased 2-10 times than that in the adjacent normal tissues. Compared with the HL-7702 cell line, LncRNA-HOST2 expression in HepG2, SMMC-7721 and Huh7 cell lines was all up-regulated, but the SMMC-7721 cell had the highest Lnc-HOST2 expression. The LncRNA-HOST2 expression in the experimental group was down-regulated as compared with the control and NC groups. In comparison with the control and NC groups, cloned cells reduced, cell apoptosis increased, clone-forming ability weakened and inhibitory rate of colony formation increased in the experimental group. The cells migrating and penetrating into the transwell chamber were fewer in the experimental group than those in the control and NC groups. The experimental group exhibited slow wound healing and decreased cell migration area after 48 h. These findings indicate that LncRNA-HOST2 can promote cell proliferation, migration and invasion and inhibit cell apoptosis in human HCC cell line SMMC-7721. 

Retracted: No

PubPeer comments:
 Figure 7A.  Effect of LncRNA-HOST2 expression on cell migration and invasion of SMMC-7721 cells detected by Transwell assays (A) Migration and invasion patterns using Transwell chamber . ![file] Fig 8(a) of [Mao et al (2018)] .  Cell invasion of bladder cancer T24 cells in the six groups (u00d7 100). (a) Results of transwell assay . ![file] Different cells, different treatments, different authors, same panel. , 
 Fig 7A again, Negative Control Invasion panel: ![] Appears to have been the original for the miR-197-3P Inhibitors NC panel of Fig 6 of [Wang et al (2016)] . ![] Here is the latter, colour reversed and superimposed on the former. Pale yellow areas are cell groups added to thie Fig 6 panel; dark blue areas are cell groups removed from the present panel; cells in both images cancel out ![] , 
 Fig 5C.  Effect of LncRNA-HOST2 expression on the proliferation of SMMC-7721 cells evaluated by clone-forming assay ... (C) Clone number Below: Fig 5 from [Wang et al (2017)] .  Cell growth (A) and colony number (B) of MG-63 cells as detected using the colony assay . ![file] UPDATE: More colors added to facilitate comparison with Fig 7A,C of [Wang et al (2019)] .  Effects of miRu2010BART7 on tumorigenicity of NSCLC cells. Notes: Au2010D, Clonogenic assay was conducted to detect colony formation after transfection in A549 (A,B) and Caluu20101 cells (C,D). ![] , 
 https: doi.org 10.1042 BSR-20160532_EOC The Editorial Office has been made aware of potential issues surrounding the scientific validity of this paper, hence has issued an expression of concern to notify readers whilst the Editorial Office investigates. , 
 Retracted [24 August 2021] . --------------------------- This Retraction follows an Expression of Concern relating to this article previously published by Portland Press. This article is being retracted from *Bioscience Reports* at the request of the Editor-in-Chief and the Editorial Board following receipt of a notification from a reader, alerting the Editorial Board to image duplications in Figures 5C and 7A which appear in other publications. The authors have been contacted in regards to the Retraction, and have not responded to the Journal s queries and the concerns raised. Given the extent of the issues raised, the Editorial Board stand by the decision to retract the article. ,