Near‐infrared emissive polymer‐coated IR ‐820 nanoparticles assisted photothermal therapy for cervical cancer cells
Authors: Mutalifu Aimaiti
Nuernisha Alifu
Jianjun Chen
Xueliang Zhang
Lijun Zhu
Shuang Chen
Rong Ma
Ting Yan
Gulinigaer Alimu
Journal: Journal of Biophotonics (2021)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: IR-820; NIR emission; cervical cancer; photothermal therapy.
Affiliations: Department of Epidemiology and Health Statistics, School of Public Health Xinjiang Medical University  Urumqi China, State Key Laboratory of Pathogenesis, Prevention, and Treatment of Central Asian High Incidence Diseases/Department of Gynecology The First Affiliated Hospital of Xinjiang Medical University  China, Central Laboratory Xinjiang Medical University  Urumqi China, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, School of Medical Engineering and Technology Xinjiang Medical University  Urumqi China

Abstract:  Photothermal therapy (PTT) has attracted wide attention due to its noninvasiveness and its thermal ablation ability. As photothermal agents are crucial factor in PTT, those with the characteristics of biocompatibility, non-toxicity and high photothermal stability have attracted great interest. In this work, new indocyanine green (IR-820) was utilized as a photothermal agent and near-infrared (NIR) fluorescence imaging nanoprobe. To improve the biocompatibility, poly(styrene-co-maleic anhydride) (PSMA) was utilized to encapsulate the IR-820 molecules to form novel IR-820@PSMA nanoparticles (NPs). Then, the optical and thermal properties of IR-820@PSMA NPs were studied in detail. The IR-820@PSMA NPs showed excellent photothermal stability and biocompatibility. The cellular uptaking ability of the IR-820@PSMA NPs was further confirmed in HeLa cells by the NIR fluorescent confocal microscopic imaging technique. The IR-820@PSMA NPs assisted PTT of living HeLa cells was conducted under 793 nm laser excitation, and a high PTT efficiency of 73.3% was obtained. 

Retracted: No

PubPeer comments:
 Figure 7. * Blue boxes: The  Control and  Control+Laser FITC photos appear to be identical * Green boxes: The  Control and  Control+Laser Cell Apoptosis plots appear to be identical, although the gated percentages are different ![file] , 
 Dear Dr. Elisabeth M Bik, Thank you for your valuable comments. According to your careful comments, we checked the whole original data, and found that the two figures were utilized with mistake for our carelessness during the typesetting. The original data of the control group with and without laser (control) were showed as follows: ![file] ![file] The original data of cell Apoptosis plots were shown as follows. 1.tControl group ![file] 2.tIR-820@PSMA NPs+laser group ![file] 3.tControl+laser group ![file] We sincerely apologize for our carelessness and we will update the data for the correct one (as follows). ![file] Figure 6 Detection of PTT in HeLa cells treated with IR-820@PSMA NPs and FITC under 793 nm laser (2 W cm2) irradiation. We have conducted this experiment very seriously. The above are the original data, and our experimental conclusions are correct in the article. Thank you for your valuable advice for our work. We will be more rigorous and serious in our future work. We appreciate your kindness. Your Sincerely Nuernisha Alifu 2021-12-28 , 
 Correction, January 2022: https: onlinelibrary.wiley.com doi 10.1002 jbio.202200004  This erratum corrects Figure 6 and Figure 5 in the above article. We recognized that the sub-Figure 6A (control by staining FITC), sub-Figure 6B (control of cell apoptosis) and sub-Figure 5 (fluorescence images for 6u2009hours) were improperly displayed. We mistakenly replicated control+laser by staining FITC as control by staining FITC in Figure 6A, control+laser of cell apoptosis as control of cell apoptosis in Figure 6B, during combination of the Figure 5, fluorescence images for 6u2009hours was not fully shown. The correct Figureu20096 is: The correct Figureu20095 is: This modification does not change the paper s results. ,