Ipriflavone promotes osteogenesis of MSCs derived from osteoporotic rats
Authors: A-G Gao
Y-C Zhou
B-B Lu
Z-J Hu
Journal: European review for medical and pharmacological sciences (2018)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: Ipriflavone; postmenopausal osteoporosis; bone quality; osteogenesis; mesenchymal stem cells
Affiliations: Department of Orthopedics, The First Affiliated Hospital Of Soochow University, Suzhou, China. .

Abstract:  Objective: To explore whether Ipriflavone could prevent postmenopausal osteoporosis (PMOP) and improve bone quality via promoting osteogenesis of bone marrow-derived mesenchymal stem cell (MSCs). Materials and methods: MSCs were extracted from rats and identified using flow cytometry. Osteogenic specific genes and adipogenic specific genes in MSCs were detected by quantitative Real-time polymerase chain reaction (qRT-PCR). The effect of Ipriflavone on osteogenesis was detected by CCK-8 (cell counting kit-8) assay, ALP activity detection, alizarin red staining and Western blot, respectively. Furthermore, ovariectomized PMOP rat model was constructed. The effects of Ipriflavone on osteogenesis, BMD and bone biomechanical properties of ovariectomized rats were detected. Results: MSCs derived from ovariectomized rats exerted multiple differentiation potentials. CCK-8 assay indicated that 0.8 μM Ipriflavone were the maximal dose that did not affect MSCs proliferation, which was selected for the following experiments. In vitro researches demonstrated that Ipriflavone remarkably promoted MSCs osteogenesis. In vivo results indicated that BMD, BV/TV, Tb.N and Tb.Th were decreased in ovariectomized rats than those of rats in sham group. Ipriflavone treatment remarkably prevented osteoporosis via promoting MSCs osteogenesis in ovariectomized rats. Conclusions: Ipriflavone prevents postmenopausal osteoporosis, improves bone quality and protects bone tissue via promoting MSCs osteogenesis. 

Retracted: No

PubPeer comments:
 This paper appears to belong to a set of (as of today) seven papers that all show very similar, and sometimes overlapping, panels with Alizarin Red-stained cells. These papers were published by different authors at different hospitals, suggesting the photos all might have originated from the same source. The papers are: * AG Gao et al., , The First Affiliated Hospital Of Soochow University, Suzhou, DOI: 10.26355 eurrev_201807_15527- https: pubpeer.com publications 65A5976CDD42166D927AF2D3BA0B56 * B Luo et al., The Affiliated Hospital (Traditional Chinese Medicine) of Southwest Medical University, Luzhou, DOI: 10.26355 eurrev_201908_18717 - https: pubpeer.com publications 20DFC925FC185C52D8BA99B153F510 * Changgong Lan et al., The First Affiliated Hospital of Jinan University, Guangzhou, DOI: 10.3892 etm.2019.8246 - https: pubpeer.com publications 1D79EA9F787AF36661079482544D12 * FB Weng et al., The Ninth Peopleu2019s Hospital of Suzhou, Suzhou, DOI: 10.26355 eurrev_201912_19759 - https: pubpeer.com publications D63F002727B3A990A4571F7D4B6F56 * GC Gao et al., The Second Affiliated Hospital of Nanchang University, Nanchang, DOI: 10.26355 eurrev_202001_20029 - https: pubpeer.com publications F04C25EBF6963C260938DF3B6BB999 * JY Li et al., Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, DOI: 10.26355 eurrev_201908_18519 - https: pubpeer.com publications 6171C86828BF72676BB2B178610093 * YC Fu et al., Health Management Center, Weifang Peopleu2019s Hospital, Weifang, DOI: 10.26355 eurrev_201908_18632 - https: pubpeer.com publications FF66C46D23036BC26668481C279E4B Here is a collage of the images from these papers. Boxes of the same color highlight panels or parts of panels that look similar. ![file] , 
 Sorry for the later response.I can confirm that the photos in our article originated from our team.We can provide the original images and data if necessary ,and we can contact the publisher to handle this situation. , 
 Dear Dr. Gao, Thank you for your reply. Yes, I d love to see some of the original photos and data here, please. ,