Silencing of PTEN inhibits the oxidative stress damage and hippocampal cell apoptosis induced by Sevoflurane through activating MEK1/ERK signaling pathway in infant rats
Authors: Bin Wang
Shubo Zhang
Tiejun Liu
Jing Bai
Xiaoliu Dong
Xiaotao Wang
Xiaojing Zhao
Wei Ma
Journal: Cell Cycle (2020)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: MEK1/ERK signaling pathway; Oxidative damages; PTEN; apoptosis; gene silencing
Affiliations: Department of Anesthesiology, North China University of Science and Technology Affiliated Hospital, Tangshan, P.R. China., Department of Neurology, Tangshan People 'S Hospital, Tangshan, P.R. China., Department of Pediatric, North China University of Science and Technology Affiliated Hospital, Tangshan, P.R. China.

Abstract:  Phosphatase and tensin homolog (PTEN) is a suppressive player in tumor but its concrete role in oxidative stress (OS) damage and cell apoptosis remains much exploration. Thus, this study is conducted to explore the participation of PTEN and its mechanisms in OS damage and cell apoptosis in hippocampal cells.Infant rats were grouped into normal, Sevo, Sevo + si-negative control (NC), Sevo + si-PTEN and Sevo + si-PTEN + PD (MEK1/ERK signaling pathway inhibitor) groups. Infant hippocampal cells were grouped into blank, Sevo, Sevo + si-NC, Sevo + si-PTEN and Sevo + si-PTEN + PD groups. The expressions of PTEN and MEK1/ERK signaling pathway-related proteins were determined. OS-related indices in hippocampal tissues and cells were detected. Cell apoptosis was detected by flow cytometry.Sevoflurane up-regulated PTEN expression and silencing of PTEN activates MEK1/ERK signaling pathway in hippocampal tissues and cells of infant rats. Silencing of PTEN alleviated hippocampal tissue pathological status and inhibited sevoflurane-induced cell apoptosis in hippocampal tissues of infant rats. Silencing of PTEN alleviated OS damage in hippocampal tissues of infant rats. Silencing of PTEN inhibited sevoflurane-induced apoptosis after OS damage in hippocampal cells of infant rats. Silencing of PTEN reduced sevoflurane-induced OS damage in hippocampal cells of infant rats.Our study demonstrates that PTEN silencing inhibits the OS damage and cell apoptosis in hippocampal cells induced by Sevoflurane through activating MEK1/ERK signaling pathway in infant rats. 

Retracted: Yes

PubPeer comments:
 Fig 2a.  Pathology of hippocampal tissues of infant rats in each group . ![file] How to explain the internal repetitions in one panel? (including many areas repeated with a 180-deg flip) ![file] ![file] , 
 Retracted, [22 February 2022] . --------------------- We, the Editors and Publisher of the journal Cell Cycle, have retracted the following article: Tiejun Liu, Xiaoliu Dong, Bin Wang, Shubo Zhang, Jing Bai, Wei Ma, Xiaojing Zhao Xiaotao Wang (2020) u2018Silencing of PTEN inhibits the oxidative stress damage and hippocampal cell apoptosis induced by Sevoflurane through activating MEK1 ERK signaling pathway in infant ratsu2019, Cell Cycle, 19:6, 684-696, DOI: http: 10.1080 15384101.2020.1717041 Since publication, concerns have been raised about the integrity of the data in the article. When approached for an explanation, the authors checked their data and confirmed there are fundamental errors present. The authors have agreed to the retraction of this article. We have been informed in our decision-making by our policy on publishing ethics and integrity and the COPE guidelines on retractions. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as u2018Retractedu2019. ,