LncRNA ASB16-AS1 promotes proliferation and inhibits apoptosis of non small cell lung cancer cells by activating the Wnt/β catenin signaling pathway
Authors: J-T Liu
L-J Tan
Y-S Zhang
M Yang
T Ju
Journal: European review for medical and pharmacological sciences (2020)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: lncRNA ASB16-AS1; non-small cell lung cancer; proliferation; apoptosis; Wnt/β-catenin signaling pathway
Affiliations: Department of Oncology, the First Affiliated Hospital of Harbin Medical University, Harbin, China. .

Abstract:  Objective: To detect the expression of long non-coding ribonucleic acid (lncRNA) ASB16-AS1 in non-small cell lung cancer (NSCLC) tissues and cells, and to explore the effect of lncRNA ASB16-AS1 on the biological functions of NSCLC cells. Patients and methods: The expression level of lncRNA ASB16-AS1 in NSCLC tissues and cells was detected via real-time fluorescence quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR). The interference sequences of lncRNA ASB16-AS1 were designed and synthesized, and its transfection efficacy was detected by qRT-PCR. After knockdown of lncRNA ASB16-AS1, the proliferation, cell cycle, and apoptosis of NSCLC cells were detected via cell counting kit-8 (CCK-8) assay, colony formation assay, and flow cytometry, respectively. Moreover, the expression changes in the Wnt/β catenin signaling pathway were detected via Western blotting. Results: LncRNA ASB16-AS1 was upregulated in NSCLC tissues and cells compared with that in paracarcinoma tissues and 16HBE cells. The results of CCK-8 assay and colony formation assay revealed that the silence of lncRNA ASB16-AS1 attenuated the proliferative ability in NSCLC. The results of flow cytometry manifested that the silence of lncRNA ASB16-AS1 arrested the cell cycle in G0/1 phase, and accelerated the apoptosis rate. The key proteins in the Wnt/β-catenin signaling pathway were regulated by lncRNA ASB16-AS1 in NSCLC. Conclusions: LncRNA ASB16-AS1 is upregulated in NSCLC tissues and cells, which promotes proliferation and inhibits apoptosis of NSCLC cells through the Wnt/β-catenin signaling pathway. 

Retracted: Yes

PubPeer comments:
 *[left]* Figure 3C.  Effects of lncRNA ASB16-AS1 on cell cycle and apoptosis of NSCLC cells. ... C, D, The results of flow cytometry showed that the apoptosis rate is increased after interference in lncRNA ASB16-AS1 expression in NSCLC cells in both groups. *[upper right]* Fig 4 from [Yu et al(2020)] .  Effect of miR-146 on apoptosis of kidney tissues . *[lower right]* Fig 5 from [Jiang et al (2020)] **(retracted)**.  Effect of miR-210 on apoptosis level in each group. ![file] Many other manifestations of these bursting-champagne-bottle scatterplots are linked in those threads, and in [a spreadsheet] . , 
 Withdrawn [some time in February 2021] .  The article u201cLncRNA ASB16-AS1 promotes proliferation and inhibits apoptosis of non small cell lung cancer cells by activating the Wnt u03b2 catenin signaling pathway, by L.-J. Tan, J.-T. Liu, M. Yang, T. Ju, Y.-S. Zhang, published in Eur Rev Med Pharmacol Sci 2020; 24 (4): 1870-1876u2013DOI: 10.26355 eurrev_202002_20365u2013PMID: 32141556u201d has been withdrawn from the authors due to due to some inaccuracies (some data cannot be repeated by our further research). The Publisher apologizes for any inconvenience this may cause. ,