MicroRNA-203 inhibits the proliferation and invasion of U251 glioblastoma cells by directly targeting PLD2
Authors: Renjun Peng
Zigui Chen
Yiqiang Cao
Xin Wan
Quan Cheng
Zhiming Ma
Rui Chen
Dazhi Li
Bing Jiang
Journal: Molecular Medicine Reports (2014)
Discipline: CLINICAL MEDICINE
Keywords: MicroRNAs; miR-203; gliomas; cell proliferation; migration
Affiliations: Department of Neurosurgery, The First Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China., Department of Neurosurgery, Traditional Chinese Medicine Hospital of Xinjiang Medical University, Urumchi, Xinjiang 830000, P.R. China.

Abstract:  MicroRNAs (miRNAs) have been demonstrated to be important in the development and progression of various types of cancer. However, the exact roles of certain anti‑oncogenic miRNAs in human malignant gliomas remain to be elucidated. The present study aimed to reveal the expression of microRNA‑203 (miR-203) in normal brain tissues and gliomas, and to investigate the role of miR-203 in cell proliferation and migration in human glioblastoma U251 cells. Real-time reverse transcription polymerase chain reaction (RT-PCR) showed that the expression of miR-203 in high WHO grade glioma tissues was significantly decreased compared with low WHO grade glioma tissues and normal brain tissues, and its expression demonstrated a decreasing tendency with ascending WHO grades. The transfection of the miR-203 mimic into U251 cells markedly downregulated the expression of phospholipase D2 (PLD2), which was identified as a direct target of miR-203. Furthermore, miR-203 overexpression significantly suppressed the proliferation and invasion of U251 cells, while the overexpression of PLD2 abrogated these effects induced by the miR-203 mimic. In conclusion, the present study demonstrated the clinical significance of miR-203 in gliomas and suggested that miR-203 was able to inhibit the proliferation and invasion of glioma cells, partially at least via suppressing the protein expression of PLD2. Thus, miR-203 may be a novel candidate for the development of therapeutic strategies for gliomas. 

Retracted: No

PubPeer comments:
 Fig 6.  PLD2 overexpression abrogated the suppressive effect of miR-203 on U251 cell invasion. Cell invasion assay was performed to determine the effects of miR-203 and PLD2 on U251 cell invasion . ![file] For comparison, Fig 3A from [Dai et al (2015)] .  A Transwell assay was performed to determine the effect of Res on cell invasion of JJ012 chondrosarcoma cells . ![file] Figs 4B and 5B from [Lei et al (2015)] .  Cell invasion assay was performed on MG63 cells with or without transfection with galectin-3-specific siRNA ;  Transwell assay was performed to examine the invasive capacity of MG63 cells transfected with galectin-3 siRNA, with or without pre-treatment of curcumin . ![file] Fig 2C from [Jaing et al (2018)] . ![file] , 
 *[below]* Fig 3A.  Western blot analysis was performed to determine the protein expression of PLD2 in 10 cases of normal brain tissues, 6 cases of WHO I glioma tissues, 7 cases of WHO II glioma tissues, 9 cases of WHO III glioma tissues as well as 9 cases of WHO IV. GAPDH was used as an internal control. Normal, normal brain tissue. *[above]* Fig 1B from [Cheng et al (2014)] . ![file] How to explain the coincidence? ,