Chi3l1 regulates APAP-induced liver injury by promoting macrophage infiltration
Authors: Y Wang
M Zhong
Y-H Li
W Wang
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: Chi3l1; Acetaminophen-induced liver injury; Inflammatory cytokines; Macrophage infiltration; Mouse model
Affiliations: Department of General Surgery, China-Japan Union Hospital of Jilin University, Changchun, China. .

Abstract:  Objective: This study aims to investigate the role of Chi3l1 in Acetaminophen (APAP)-induced liver injury. Materials and methods: In vivo model of liver injury was established in mice administrated with APAP (250 mg/kg) or equivalent phosphate-buffered saline (PBS). Mouse liver tissues were collected at 1 h, 3 h, 6 h, 12 h, and 24 h after treatment, respectively. ALT levels and apoptosis were evaluated. Additionally, we established APAP-induced acute liver injury model in wild-type (WT) mice and Chi3l1-deficient (Chi3l1-/-) mice. Pathological changes of liver tissue were observed by hematoxylin and eosin (HE) staining. Mononuclear cells (MNCs) were isolated from mouse liver, and the amounts of infiltrating macrophages and neutrophils were then counted by flow cytometry. Serum levels of cytokines were detected by enzyme-linked immunosorbent assay (ELISA). Bone marrow-derived macrophages (BMDMs) were extracted from each mouse. Results: After APAP stimulation, Chi3l1-/- mice showed more severe liver injury than that of WT mice, which was manifested as higher ALT levels and more necrotic or apoptotic cells. Compared with WT mice, Chi3l1-/- mice expressed higher levels of inflammatory cytokines (MCP-1 and IL-6), macrophage-associated molecules (CD68 and CD86), as well as the amounts of infiltrating macrophages and neutrophils. In addition, higher expressions of inflammatory cytokines were found in BMDMs extracted from WT mice treated with those BMDM lysates derived from Chi3l1-/- mice than those of non-treated cells. APAP-treated Chi3l1-/- mice exhibited more severe liver injury than that of WT mice. Conclusions: Our study confirmed that Chi3l1 protects the liver function from APAP-induced injury by inhibiting the secretion of inflammatory factors and macrophage infiltration. 

Retracted: Yes

PubPeer comments:
  Figure 2B. **Chi3l1 knockout exacerbated** APAP-induced liver damage in mice. ... B, HE staining micrographs (magnification 100u00d7, 200u00d7) of liver tissues of WT and Chi3l1- - mice at 8 h and 24 h; significant hepatocyte necrosis was observed in Chi3l1- - mice (black arrows). ![file] Figure 2B from [Cheng et al (2018)] .  **CD31 knockout protected** mice from APAP-induced liver injury. ... B, HE staining images (100u00d7) of liver tissues in WT and CD31- - mice at 8 and 24 h. A large number of hepatocyte necrosis was observed in WT mice, whereas CD31- - mice had less hepatic damage. ![file] These are versatile microphotographs. , 
 #1 thank you for your adviceuff0cI will check them then explain it to you , 
 Withdrawn without explanation. https: www.europeanreview.org article 23171 *The article u201cChi3l1 regulates APAP-induced liver injury by promoting macrophage infiltration, by Y. Wang, M. Zhong, W. Wang, Y.-H. Li, published in Eur Rev Med Pharmacol Sci 2019; 23 (11): 4996-5003u2013DOI: 10.26355 eurrev_201906_18091u2013PMID: 31210337u201d has been withdrawn from the authors.* *The Publisher apologizes for any inconvenience this may cause.* The companion paper using the same images ([Cheng et al 2018] ) is unaffected. , 
 Wrong thread; comment deleted. ,