Dinitrosopiperazine-decreased PKP3 through upregulating miR-149 participates in nasopharyngeal carcinoma metastasis
Authors: Haitao Xie
Yuejin Li
Kunyu Ju
Zheliang Liu
Jinping Lu
Yuan Jiang
Faqing Tang
Guanmin Jiang
Zigang Dong
Weiwei Wang
Journal: Molecular Carcinogenesis (2018)
Discipline: CLINICAL MEDICINE
Keywords: N,N′-dinitrosopiperazine; Plakophilin3; metastasis; miR-149; nasopharyngeal carcinoma.
Affiliations: Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China., Zhuhai Hospital of Jinan University, Zhuhai, China., Metallurgical Science and Engineering, Central South University, Changsha, China., The First Affiliated Hospital of University of South China, Hengyang, China., Hormel Institute, University of Minnesota, Austin, Minnesota.

Abstract:  Nasopharyngeal carcinoma (NPC) has a high metastatic clinicopathological feature. As a carcinogen factor, N,N'-dinitrosopiperazine (DNP) is involved in NPC metastasis, but its precise mechanism has not been fully elucidated. Herein, we showed that DNP promotes NPC metastasis through upregulating miR-149. DNP was found to decrease Plakophilin3 (PKP3) expression, further DNP-decreased PKP3 was verified to be through upregulating miR-149. We also found that DNP induced proliferation, adhesion, migration and invasion of NPC cell, which was inhibited by miR-149-inhibitor. DNP may promote NPC metastasis through miR-149-decreased PKP3 expression. Therefore, DNP-increased miR-149 expression may be an important factor of NPC high metastasis, and miR-149 may serve as a molecular target for anti-metastasis therapy of NPC. 

Retracted: No

PubPeer comments:
 Figure 8 has two pictures which appear in multiple articles with overlapping authors. This is part of a larger group of articles with numerous duplications. ![file] Dinitrosopiperazine-decreased PKP3 through upregulating miR-149 participates in nasopharyngeal carcinoma metastasis, Mol Carcinog. 2018 Dec, 57(12): 1763u2013-1779, doi:u00a010.1002 mc.22895, Figure 8 ![file] Berberine Inhibits Metastasis of Nasopharyngeal Carcinoma 5-8F Cells by Targeting Rho Kinase-mediated Ezrin Phosphorylation at Threonine 567, J Biol Chem. 2009 Oct 2; 284(40): 27456u2013-27466, doi:u00a010.1074 jbc.M109.033795, Figure 4 ![file] N,Nu0001-Dinitrosopiperazine-mediated Ezrin Protein Phosphorylation via Activation of Rho Kinase and Protein Kinase C Is Involved in Metastasis of Nasopharyngeal Carcinoma 6-10B Cells, J Biol Chem. 2011 Oct 2; 286(42): 36956--36967, doi:u00a010.1074 jbc.M111.259234, Figure 5 , 
 There is a third image in the same figure which also appears in multiple articles. ![file] Dinitrosopiperazine-decreased PKP3 through upregulating miR-149 participates in nasopharyngeal carcinoma metastasis, Mol Carcinog. 2018 Dec, 57(12): 1763u2013-1779, doi:u00a010.1002 mc.22895, Figure 8B ![file] MNAT1 is overexpressed in colorectal cancer and mediates p53 ubiquitindegradation to promote colorectal cancer malignance, J Exp Clin Cancer Res. 2018; 37:284, doi:u00a010.1186 s13046-018-0956-3, Figure 8. , 
 And there is a fourth image, from the same figure, which appears in multiple articles. ![file] Dinitrosopiperazine-decreased PKP3 through upregulating miR-149 participates in nasopharyngeal carcinoma metastasis, Mol Carcinog. 2018 Dec, 57(12): 1763u2013-1779., doi:u00a010.1002 mc.22895, Figure 8B ![file] Dinitrosopiperazine-Mediated Phosphorylated-Proteins Are Involved in Nasopharyngeal Carcinoma Metastasis, Int. J. Mol. Sci. 2014 Nov; 15(11), 20054--20071, doi:u00a010.3390 ijms151120054, Figure 6 , 
 One of the images in Figure two appears in multiple articles. ![file] Dinitrosopiperazine-decreased PKP3 through upregulating miR-149 participates in nasopharyngeal carcinoma metastasis, Mol Carcinog. 2018 Dec, 57(12): 1763u2013-1779, doi:u00a010.1002 mc.22895, Figure 2. ![file] Clusterin induced by N,Nu2019-Dinitrosopiperazine is involved in nasopharyngeal carcinoma metastasis, Oncotarget. 2016 Feb 2; 7(5): 5548--5563, doi:u00a010.18632 oncotarget.6750, Figure 6. , 
 #4 There seems to be at least one additional overlap between the Oncotarget paper and this one. There are common authors on both papers. I do believe these particular images are described similarly as untreated 5-8F cells. I added the email address for the corresponding author and will email the journal. *** ![file] ,