Correlations of IGF-1R and COX-2 Expressions with Ras and BRAF Genetic Mutations, Clinicopathological Features and Prognosis of Colorectal Cancer Patients
Authors: Zi-Wen Long
Xiang Lin
Mei Jin
Jing Yang
Journal: Pathology & Oncology Research (2018)
Discipline: CLINICAL MEDICINE
Keywords: BRAF mutation; Clinicopathological feature; Cyclooxygenase 2; Insulin-like growth factor receptor 1; Prognosis
Affiliations: Department of Surgery, Shigatse People's Hospital, Shigatse, Tibet, 85700, People's Republic of China., Department of Gastric Cancer Surgery, Fudan University Shanghai Cancer Center, Shanghai, 200032, People's Republic of China. ., Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, People's Republic of China. .

Abstract:  This case-control study aims to investigate the correlations of insulin-like growth factor receptor 1 (IGF-1R) and cyclooxygenase 2 (COX-2) expressions with Ras and BRAF genetic mutations, clinicopathological features and prognosis of colorectal cancer (CRC) patients. A total of 213 CRC patients (case group) and 200 healthy individuals (control group) were selected from our hospital. Ras (K-Ras/N-Ras) and BRAF genetic mutations were detected by direct sequencing. The positive expression rates of IGF-IR and COX-2 in CRC and normal tissues were detected using immunohistochemistry. RT-qPCR and Western blotting were applied to detect the mRNA and protein expressions of IGF-IR and COX-2 in CRC tissues and normal tissues. Total mutation rate of N-Ras, BRAF and K-Ras in case group were 5.2%, 12.2% and 47.4%, respectively. The expressions of IGF-IR and COX-2 were higher in CRC tissues with Ras and BRAF mutations than in those without. CRC tissues with Ras mutation showed higher COX-2 expression than those with BRAF mutation. IGF-IR and COX-2 expressions were correlated to infiltration degree, lymphatic metastasis (in CRC tissues with and without Ras and BRAF mutations), and Dukes stages (only in CRC tissues with Ras and BRAF mutations). CRC patients with negative expressions of IGF-IR and COX-2 had significantly higher accumulative survival rate and longer mean survival duration than those with positive expressions of IGF-IR and COX-2. These findings indicate that IGF-1R and COX-2 expressions may be associated with Ras and BRAF genetic mutations, clinicopathological feature and prognosis of CRC patients. 

Retracted: No

PubPeer comments:
 Fig 2A:  IGF-IR and COX-2 expressions in normal tissues and colorectal cancer tissues. Note: (a) Immunohistochemistry of IGF-IR and COX-2 expressions in normal tissues and colorectal cancer tissues . ![file] Wild-type IGF-IR is simply not plausible as a biological image. As well as the long diagonal band of repeated texture, I have marked a few duplicated areas. ![file] , 
 Fig 3.  Pearsonu2019s correlation analysis of the protein and mRNA expressions of IGF-IR and COX-2 with colorectal cancer patients with different genetic mutations . ![file] Each scatterplot is dominated by a neatly-aligned linear ogival sequence of points surrounded by a halo of more random points dragging down the regression value. This is not biologically plausible. , 
 #1 #2 Thank you for your question.I checked my records and data immediately.Thank you for your correction. ,