Circular RNA CircCDYL Regulates Proliferation and Apoptosis in Non-Small Cell Lung Cancer Cells by Sponging miR-185-5p and Upregulating TNRC6A
Authors: Li-Yan Wang
Feng Xue
Wei-Xin Bian
Xiao-Fang Xing
Journal: Cancer Management and Research (2021)
Discipline: CLINICAL MEDICINE
Keywords: apoptosis; circular RNA; non-small cell lung cancer; proliferation.
Affiliations: Department of Oncology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang Province 150001, People's Republic of China., Department of Oncology, Affiliated Hospital of Guilin Medical College, Guilin, Guangxi Province 541001, People's Republic of China.

Abstract:  Aim: A series of research reveal that circular RNA (circRNA) plays a vital role in regulating the development of tumor cells. In this research, we would explore the role and mechanism of circCDYL in non-small cell lung cancer (NSCLC). Methods: RT-PCR was performed to detect the expression of circCDYL in NSCLC tissues, plasma, and cell lines. The tumor cell proliferation ability was evaluated by clone formation assay, and cell cycle determination. Flow cytometry was used to detect apoptosis in NSCLC cell lines. Western blot and RT-PCR were used to assess the expression of proteins and genes. Luciferase assay was performed to confirm the relationship of circRNA-miRNA-mRNA. Results: The decreased level of circCDYL was observed in NSCLC patients' tissues and plasma, which was also downregulated in NSCLC cell lines. Forced expression of circCDYL inhibited cell viability, proliferation and induced apoptosis in A549 cells. Luciferase assay verified that circCDYL could bind with miR-185-5p and confirmed that TNRC6A was a downstream target of miR-185-5p. Overexpression of miR-185-5p or silencing of TNRC6A could inhibit the anti-tumor effect of circCDYL in A549 cells via regulating the ERK1/2 signal. Conclusion: Here, we revealed that circCDYL inhibited proliferation and induced apoptosis in NSCLC cell lines via regulating ERK1/2 signal, and the mechanism of this progression may target miR-185-5p/TNRC6A, which provided a theoretical basis for clinical therapy. 

Retracted: No

PubPeer comments:
 In the closing paragraphs we read * Our findings signified the circ_0000376 high expression in NSCLC and its clinical significance. We also found that circ_0000376 enhances the proliferation, invasion, and metastasis in NSCLC cells via modulating miR-384. To our best knowledge, this is the first study to investigate the role of circ_0000376 in cancer biology and it is expected to provide new therapeutic targets for NSCLC patients *. This conclusion is unexpected in a paper than otherwise concentrates on CircCDYL. The source appears to be the last paragraph of [Sun et al (2020)] . * Collectively, our findings signified the circ_0000376 high expression in NSCLC and its clinical significance. We also found that circ_0000376 enhances the proliferation, invasion, and metastasis in NSCLC cells via modulating miR-384. To our best knowledge, this is the first study to investigate the role of circ_0000376 in cancer biology and it is expected to provide new therapeutic targets for NSCLC patients. * Perhaps the authors intended to transpose the sentences into third-person? , 
 Expression of COncern, [26 October 2021] -------------------------- The Editor and Publisher of *Cancer Management and Research* wish to issue an Expression of Concern for the published article. Following publication, concerns were raised about the scientific integrity of the articles content. We reached out to the authors requesting they supply information that would confirm the integrity of the data. However, the authors did not provide the requested files and could not satisfy our requirements to confirm the authenticity of some types of data. Therefore, as we continue to work through the issues raised, we advise readers to interpret the information presented in the article with due caution. The authors have been notified about this Expression of Concern. , 
 - *[left]* Fig 5D. - *[right]* Fig 5D from  MicroRNA-802 Inhibits Cell Proliferation and Induces Apoptosis in Human Laryngeal Cancer by Targeting cAMP-Regulated Phosphoprotein 19 ([Ye et al 2020] ) **[retracted]**. ![file] , 
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