Long noncoding RNA DUXAP8 contributes to the progression of hepatocellular carcinoma via regulating miR‐422a/PDK2 axis
Authors: Jing Zhang
Liang Yang
Feifei Wei
Guiyan Tang
Dandan Jiang
Min Pan
Mingyue Huang
Journal: Cancer medicine (2020)
Discipline: CLINICAL MEDICINE
Keywords: DUXAP8; HCC; PDK2; miR-422a.
Affiliations: Department of Oncology, Fifth Clinical Medical College, Guilin Medical University, Guilin, People's Republic of China., Department of Oncology, Jining NO.1 People's Hospital; Affiliated Jining NO.1 People's Hospital, Jining Medical University, Jining, People's Republic of China.

Abstract:  Background: Hepatocellular carcinoma (HCC) is one of the most deadly cancer worldwide. Multiple long noncoding RNAs (lncRNAs) are recently identified as crucial oncogenic factors or tumor suppressors. In this study, we explored the functon and mechanism of lncRNA double homeobox A pseudogene 8 (DUXAP8) in the progression of HCC. Methods: Expression levels of DUXAP8 in HCC tissue samples were measured using qRT-PCR. The association between pathological indexes and the expression of DUXAP8 was also analyzed. Human HCC cell lines SMMC-7721 and QSG-7701 were used in in vitro studies. CCK-8 assay was used to assess the effect of DUXAP8 on HCC cell line proliferation. Scratch healing assay and Transwell assay were conducted to detect the effect of DUXAP8 on migration and invasion. Furthermore, dual-luciferase reporter assay was used to confirm targeting relationship between miR-422a and DUXAP8. Additionally, Western blot was used to detect the regulatory function of DUXAP8 on pyruvate dehydrogenase kinase 2 (PDK2). Results: DUXAP8 expression HCC clinical samples was significantly increased and this was correlated with unfavorable pathological indexes. High expression of DUXAP8 was associated with shorter overall survival time of patients. Its overexpression remarkably facilitated the proliferation, metastasis, and epithelial-mesenchymal transition of HCC cells. Accordingly, knockdown of it suppressed the malignant phenotypes of HCC cells. Overexpression of DUXAP8 significantly reduced the expression of miR-422a by sponging it, but enhanced the expression of PDK2. Conclusions: DUXAP8 was a sponge of tumor suppressor miR-422a in HCC, enhanced the expression of PDK2 indirectly, and functioned as an oncogenic lncRNA. 

Retracted: No

PubPeer comments:
 In Figure 2D, a micrograph has been duplicated and presented as something else (Invation, 2nd an 3rd image from left). In Figure 5C, two cases where micrographs have been duplicated and presented as something else. In addition they have been manipulated (different size slight rotation; Left panel: Invasion-control vs Migration-2nd image from left; Right panel: Migration-2nd image from left vs Left panel, Migration-2nd image from left). ![file] , 
 #1 Thank you for your comments. These comments are very important to us. We must apologize for our inadvertent mistakes. When we performed Transwell assays, we took a lot of photos. When we were preparing the figures, the wrong images were selected, captured and presented. After receiving your comments, we have checked the raw photos, and corrected Figure 2 and Figure 5. Please check it. We will also try to contact with the journal to make a correction. Thank you again for your comments. Jiang Dandan , 
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