Long noncoding RNA ANRIL contributes to the development of ulcerative colitis by miR-323b-5p/TLR4/MyD88/NF-κB pathway
Authors: Lili Yang
Jine Wan
Cuixia Qiao
Chengjian Pang
Gang Zhao
Wenli You
Xiaoling Liu
Journal: Biochemical and biophysical research communications (2019)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: ANRIL; TLR4/MyD88/NF-κB pathway; Ulcerative colitis; miR-323b-5p.
Affiliations: Department of Anorectal, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003, China; School of First Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210023, China., School of First Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 210023, China; Jingwen Library, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China., Department of High Pressure Oxygen, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003, China., Department of Obstetrics, Qingdao Women and Children's Hospital, Qingdao, Shandong, 266000, China., Department of Anorectal, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003, China., Department of Anorectal, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003, China. Electronic address: .

Abstract:  The aim of this study was to investigate the role and possible mechanism of long noncoding RNA ANRIL in the development of ulcerative colitis (UC). The expression of ANRIL in colonic mucosa tissues collected from the sigmoid colon of UC patients and healthy control was determined. Subsequently, fetal human cells (FHCs) were treated with lipopolysaccharide (LPS) to stimulate UC-caused inflammatory injury, followed by detection of the effects of suppression of ANRIL on cell viability, apoptosis and cytokines production in LPS-stimulated FHCs. Moreover, the regulatory relationship between ANRIL and miR-323b-5p as well as the target relationship between miR-323b-5p and TLR4 were investigated. Furthermore, the effects of ANRIL/miR-323b-5p axis on the activation of TLR4/MyD88/NF-κB pathway in LPS-stimulated FHCs were investigated. LncRNA ANRIL was highly expressed in colonic mucosa tissues of UC patients. In addition, LPS markedly induced cell injury in FHC cells (inhibited cell viability and promoted cell apoptosis and cytokine production). Suppression of ANRIL alleviated LPS-induced injury in FHC cells, which was achieved by negatively regulating miR-323b-5p. Moreover, miR-323b-5p negatively regulated TLR4 expression and TLR4 was a target of miR-323b-5p. Knockdown of TLR4 reversed the effects of miR-323b-5p suppression on LPS-induced injury in LPS-stimulated FHCs. Furthermore, the effects of ANRIL on LPS-induced cell injury were achieved by TLR4/MyD88/NF-κB pathway. Our data indicate that suppression of ANRIL may inhibit the development of UC by regulating miR-323b-5p/TLR4/MyD88/NF-κB pathway. ANRIL/miR-323b-5p/TLR4/MyD88/NF-κB pathway may provide a new strategy for UC therapy. 

Retracted: No

PubPeer comments:
 Could the authors please double check figure 2? There may be a repeated graph. *** ![file] , 
 April 6, 2021 The Editorial Office has been made aware of potential issues surrounding the scientific validity of this paper, hence has issued an expression of concern to notify readers whilst the Editorial Office investigates. , 
 Corrected [1 July 2021] . ------------------------------- This Correction follows an Expression of Concern relating to this article previously published by Portland Press. The Authors of the original article u201cPrognostic values of inhibitory u03baB kinases mRNA expression in human gastric canceru201d (Biosci Rep (2019) 39(1); DOI: 10.1042 BSR20180617) would like to correct their Figure 2 graphs. The Authors state that due to an error in the initial drafting process of the article, a graph was accidentally duplicated in parts B and C of Figure 2. This duplication had not been identified at an earlier stage, and was therefore carried through to the publication. The correct Figure 2 graphs are presented here. The Authors declare that this error has no effect on the conclusions of their study. ![file] ,