<p>miR-302e Suppresses Glioma Progression by Targeting VEGFA</p>
Authors: Tiemin Hu
Weixing Wang
Yunpeng Xie
Xin Liu
Journal: Cancer Management and Research (2020)
Discipline: CLINICAL MEDICINE
Keywords: VEGFA; glioma; miR-302e; migration; proliferation.
Affiliations: Department of Neurosurgery, Chengde Medical College Affiliated Hospital, Chengde, Hebei Province, People's Republic of China., Department of Oncology, Chengde Medical College Affiliated Hospital, Chengde, Hebei Province, People's Republic of China.

Abstract:  Background: MiRNA can be involved in regulating tumor genesis and development by regulating the expression of specific genes and regulating corresponding signaling pathways. In this study, we explored the function and mechanisms of miR-302e in glioma progression. Methods: Experimental methods include the following: real-time quantitative PCR, Western Blot Analysis, CCK8 assay and detection of apoptosis. Results: MiR-302e was down-regulated in cancer tissues and cell lines, and the expression of miR-302e was negatively correlated with the tumor grade, which indicated poor prognosis in glioma patients. Followed functional analysis showed overexpression of miR-302e inhibited proliferation, migration and invasion but promoted apoptosis of glioma cells, while silencing miR-302e showed the opposite effects. Mechanistic studies have shown that VEGFA was a directed target of miR-302e. Forced expression of VEGFA removed the inhibiting impact of miR-302e on glioma development. In vivo tumorigenesis experiments showed that miR-302e suppressed glioma development by targeting VEGFA. Conclusion: Present study emphasized miR-302e suppressed glioma development by targeting VEGFA, which might be a valuable target for glioma treatment. 

Retracted: Yes

PubPeer comments:
 16 March 2021  The Editor-in-chief and Publisher of Cancer Management and Research wish to retract the published article. It was bought to the journalu2019s attention that some images in the article appeared to be duplicated with those from another article, Xu et al: Transcriptional regulation of miR-483-3p mediated by IL-6 STAT3 axis promoted epithelialmesenchymal transition and tumor stemness in glioma . u25cf Figure 1D in the published article appears to be identical to Figure 1D in Xu et al but the data represents different experimental conditions and samples. u25cf Figure 7E in the published article appears to be identical to Figure 8A in Xu et al but the data represents different experimental conditions and samples. We attempted to contact the authors of this article but despite multiple attempts received no response. The Editor has determined the findings of the study are no longer valid and requested for the article to be retracted. Our decision-making was informed by our policy on publishing ethics and integrity and the COPE guidelines on retraction. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as u201cRetractedu201d.  *** I would note that the corresponding author s email address (yinyuti00377360@163.com) bears no resemblance to their name (Weixing Wang). This is sometimes seen on paper mill papers. , 
 https: twitter.com DavidSandersRep status 1385241718082220038 *[right]* Fig 1D.  The overall survival of glioma patients with low or high expression of miR-302e in glioma tissues were assessed by Kaplan-Meier survival analysis. *[left]* Fig 1D from [Xu, Chi Xu (2020)] .  The 5-year survival rate of high and low miR483-3p level [glioma] patients . ![file] They appear to be the same. , 
 *[right]* Figs 7B, 7F.  Tumor weight was determined in the isolated tumors from the nude mice . *[left]* Fig 8B from Xu, Chi Xu (2020).  Tumor weight was increased after miR-483-3p overexpression . ![file] The resolution does not allow comparison of the tumors, but the ruler seems to be the same. ,