Integrated Myofibrillar Protein Synthesis in Recovery From Unaccustomed and Accustomed Resistance Exercise With and Without Multi-ingredient Supplementation in Overweight Older Men
Authors: Tim Snijders
Kirsten E Bell
Dinesh Kumbhare
Stuart M Phillips
Philip J Atherton
Ken Smith
Gianni Parise
Matthew S Brook
Journal: Frontiers in Nutrition (2019)
Discipline: CLINICAL MEDICINE
Keywords: creatine; deuterated water; fractional synthesis rate; high-intensity interval training; n-3 PUFA
Affiliations: Department of Kinesiology, University of Waterloo, Waterloo, ON, Canada., School of Life Sciences, University of Nottingham, Nottingham, United Kingdom., Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, Netherlands., Department of Medicine, University of Toronto, Toronto, ON, Canada., Exercise Metabolism Research Group, Department of Kinesiology, McMaster University, Hamilton, ON, Canada., School of Graduate Entry Medicine and Health, University of Nottingham, Derby, United Kingdom.

Abstract:  Background: We previously showed that daily consumption of a multi-ingredient nutritional supplement increased lean mass in older men, but did not enhance lean tissue gains during a high-intensity interval training (HIIT) plus resistance exercise training (RET) program. Here, we aimed to determine whether these divergent observations aligned with the myofibrillar protein synthesis (MyoPS) response to acute unaccustomed and accustomed resistance exercise. Methods: A sub-sample of our participants were randomly allocated (n = 15; age: 72 ± 7 years; BMI: 26.9 ± 3.1 kg/m2 [mean ± SD]) to ingest an experimental supplement (SUPP, n = 8: containing whey protein, creatine, vitamin D, and n-3 PUFA) or control beverage (CON, n = 7: 22 g maltodextrin) twice per day for 21 weeks. After 7 weeks of consuming the beverage alone (Phase 1: SUPP/CON only), subjects completed 12 weeks of RET (twice per week) + HIIT (once per week) (Phase 2: SUPP/CON + EX). Orally administered deuterated water was used to measure integrated rates of MyoPS over 48 h following a single session of resistance exercise pre- (unaccustomed) and post-training (accustomed). Results: Following an acute bout of accustomed resistance exercise, 0-24 h MyoPS was 30% higher than rest in the SUPP group (effect size: 0.86); however, in the CON group, 0-24 h MyoPS was 0% higher than rest (effect size: 0.04). Nonetheless, no within or between group changes in MyoPS were statistically significant. When collapsed across group, rates of MyoPS in recovery from acute unaccustomed resistance exercise were positively correlated with training-induced gains in whole body lean mass (r = 0.63, p = 0.01). Conclusion: There were no significant between-group differences in MyoPS pre- or post-training. Integrated rates of MyoPS post-acute exercise in the untrained state were positively correlated with training-induced gains in whole body lean mass. Our finding that supplementation did not alter 0-48 h MyoPS following 12 weeks of training suggests a possible adaptive response to longer-term increased protein intake and warrants further investigation. This study was registered at ClinicalTrials.gov. Clinical Trial Registration: www.ClinicalTrials.gov, identifier: NCT02281331. 

Retracted: No

PubPeer comments:
 Copy pasted from https: www.frontiersin.org articles 10.3389 fnut.2020.611389 full :  A Corrigendum on Integrated Myofibrillar Protein Synthesis in Recovery From Unaccustomed and Accustomed Resistance Exercise With and Without Multi-ingredient Supplementation in Overweight Older Men by Bell, K. E., Brook, M. S., Snijders, T., Kumbhare, D., Parise, G., Smith, K., et al. (2019). Front. Nutr. 6:40. doi: 10.3389 fnut.2019.00040 In the original article, there was an error in Conflict of Interest Statement. The updated statement appears below. The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated. Conflict of Interest Statement SP is listed as an inventor on patent (Canadian) 3052324 issued to Exerkine, and a patent (US) 16 182891 pending to Exerkine (but reports no financial gains). SP reports personal fees from Enhanced Recovery (donated to charity), equity from Exerkine (all proceeds donated to charity), outside the submitted work. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Received: 29 September 2020; Accepted: 30 September 2020; Published: 30 October 2020. See https: retractionwatch.com 2021 02 12 eleven-papers-corrected-after-nutrition-prof-fails-to-disclose-patent-company-ties for backgrounds. ,