Dissociated expression of natural killer 1.1 and T-cell receptor by invariant natural killer T cells after interleukin-12 receptor and T-cell receptor signalling
Authors: Masashi Emoto
Robert Hurwitz
Stefan H E Kaufmann
Hiromi Koike
Yoshiko Emoto
Takamitsu Shimizu
Izumi Yoshizawa
Journal: Immunology (2010)
Discipline: IMMUNOLOGY
Keywords: Invariant natural killer T cells; iNKT cells; alpha-galactosylceramide; alpha-GalCer; interleukin-12
Affiliations: Laboratory of Immunology, Department of Laboratory Sciences, Gunma University School of Health Sciences, Maebashi, Gunma, Japan. 

Abstract:  Invariant (i) natural killer T (NKT) cells become undetectable after stimulation with alpha-galactosylceramide (alpha-GalCer) or interleukin (IL)-12. Although down-modulation of surface T-cell receptor (TCR)/NKR-P1C (NK1.1) expression has been shown convincingly after stimulation with alpha-GalCer, it is unclear whether this also holds true for IL-12 stimulation. To determine whether failure to detect iNKT cells after IL-12 stimulation is caused by dissociation/internalization of TCR and/or NKR-P1C, or by block of de novo synthesis of these molecules, and to examine the role of IL-12 in the disappearance of iNKT cells after stimulation with alpha-GalCer, surface (s)/cytoplasmic (c) protein expression, as well as messenger RNA (mRNA) expression of TCR/NKR-P1C by iNKT cells after stimulation with alpha-GalCer or IL-12, and the influence of IL-12 neutralization on the down-modulation of sTCR/sNKR-P1C expression by iNKT cells after stimulation with alpha-GalCer were examined. The s/cTCR(+ )s/cNKR-P1C(+) iNKT cells became undetectable after in vivo administration of alpha-GalCer, which was partially prevented by IL-12 neutralization. Whereas s/cNKR-P1C(+) iNKT cells became undetectable after in vivo administration of IL-12, s/cTCR(+) iNKT cells were only marginally affected. mRNA expression of TCR/NKR-P1C remained unaffected by alpha-GalCer or IL-12 treatment, despite the down-modulation of cTCR and/or cNKR-P1C protein expression. By contrast, cTCR(+ )cNKR-P1C(+) sTCR(-) sNKR-P1C(-) iNKT cells and cNKR-P1C(+) sNKR-P1C(-) iNKT cells were detectable after in vitro stimulation with alpha-GalCer and IL-12, respectively. Our results indicate that TCR and NKR-P1C expression by iNKT cells is differentially regulated by signalling through TCR and IL-12R. They also suggest that IL-12 participates, in part, in the disappearance of iNKT cells after stimulation with alpha-GalCer by down-modulating not only sNKR-P1C, but also sTCR. 

Retracted: Yes

PubPeer comments:
 2020 retraction. https: onlinelibrary.wiley.com doi abs 10.1111 imm.13244 Retraction: Emoto, M., Shimizu, T., Koike, H., Yoshizawa, I., Hurwitz, R., Kaufmann, S.H.E. and Emoto, Y. (2010), Dissociated expression of natural killer 1.1 and Tu2010cell receptor by invariant natural killer T cells after interleukinu201012 receptor and Tu2010cell receptor signalling. Immunology, 129: 62u201374. https: doi.org 10.1111 j.1365-2567.2009.03148.x The above article, published online on 08 December 2009 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the Editoru2010inu2010Chief of Immunology and John Wiley Sons Ltd. The retraction has been agreed following an investigation carried out by Gunma University (http: www.gunma-u.ac.jp wp-content uploads 2017 10 chosakekka29.pdf). The investigation was unable to determine the validity of the images for which Professor Emoto, the article s corresponding author, was responsible. As a result, the journal has made the decision to retract the article. ,