miRNA‑183‑5p.1 promotes the migration and invasion of gastric cancer AGS cells by targeting TPM1
Authors: Jun Lin
Juan Shen
Hao Yue
Zhongwei Cao
Journal: Oncology Reports (2019)
Discipline: CLINICAL MEDICINE
Keywords: MicroRNA-183; Gastric cancer; Oncogene; Migration; Tropomyosin 1 (TPM1)
Affiliations: Department of Pathology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, P.R. China, Department of Gastroenterology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, P.R. China

Abstract:  MicroRNA‑183 (miR‑183) is a small, non‑coding RNA that is involved in post‑transcriptional processes, is upregulated in gastric cancer and acts as an oncogene in cancer migration. Although fragmentary reports have demonstrated the importance of miR‑183 in gastric cancer, its biofunctions and regulatory effects are still unknown. In the present study, the gene and protein expression levels were determined by reverse transcription‑quantitative PCR and western blot analysis. The connection between miR‑183‑5p.1 and tropomyosin 1 (TPM1) was tested through luciferase reporter experiments. Cell viability, apoptosis and related proteins were detected by MTT assay, flow cytometry, immunofluorescence and western blotting, respectively. The migration and invasion of AGS cells modulated by miR‑183‑5p.1 were analyzed by Transwell assay. TPM1 expression was found to be decreased in gastric cancer tissues and cell lines when compared with normal and adjacent tissues and gastric epithelial cells, and was regulated by miR‑183‑5p.1 targeting TPM1. miR‑183‑5p.1 overexpression facilitated the growth and suppressed the death of AGS cells through Bcl‑2 and P53 proteins. In addition, miR‑183‑5p.1 restricted TPM1, TPM2 and TPM3 protein expression in AGS cells. The excessive levels of miR‑183‑5p.1 promoted the migration and invasion of AGS cells, and inhibited the apoptosis of AGS cells. However, the knockdown of miR‑183‑5p.1 induced the opposite in AGS cells. In conclusion, miR‑183‑5p.1 promotes cell proliferation, migration and invasion by downregulating TPM1 and deactivating the Bcl‑2/P53 signaling pathways in gastric cancer, indicating that miR‑183‑5p.1 and TPM1 may be potential targets for the diagnosis or therapy of gastric cancer in the future. 

Retracted: No

PubPeer comments:
 This paper is one of a set of 121 papers that all contain images from the same library of about 100 photos and plots. This is unexpected, because each paper has different authors and different affiliations, with little to no overlap, and describes different cancer types and molecules. It would be very hard to explain how authors from different labs would all obtain the same photo. Therefore, the papers might have all be derived from the same source, such as a paper mill that sells ready-to-be-published papers. This  Stock Photo paper set has been described in more detail on my blog post, https: scienceintegritydigest.com 2020 07 05 the-stock-photo-paper-mill and in a Wall Street Journal article by Eva Xiao (currently behind a paywall): https: www.wsj.com articles chinese-research-papers-raise-doubts-fueling-global-questions-about-scientific-integrity-11593939600 The vast majority (113 121) of the papers were published in the same journal, European Review for Medical and Pharmacological Sciences (ERMPS). A link to the full set of papers can be found here: https: docs.google.com spreadsheets d 172RWS18w1lTNJ9Nnj80fuXXTqTm4fGkoK0sdYyvKE3g edit?usp=sharing All papers have been reported to the journal editors on July 2, 2020. , 
 Hello, thank you for your attention, I am collating data, later reply you , 
 #1 Based on the information included on your spreadsheet, I marked some of the overlaps of images of this paper with others. There are more. ![] ![] , 
 My article was published before your list , 
 #4 Dr. Zhao: Thank you for your reply. Iu2019m not sure I understand what you are saying. Are you suggesting that your paper was published before the others? In any case, I donu2019t think that you have addressed the concern described by Dr. Bik in #1. , 
 Dear Dr. Zhao, Thank you for your reply. I am equally confused as Actinopolyspora in comment #5. Of course, your paper was published before I made a list of the Stock Photo papers. Your paper was not the first to publish these images, nor was it the last. It just appears to be part of a set of 121 papers that all use from the same library of photos. I am happy to show the details of the findings in your paper, and link to the spreadsheet with all the papers, so you can check if my numbers are correct: https: scienceintegritydigest.files.wordpress.com 2020 07 stockphoto-papermill-july-2-2020-public-version.xlsx or (in Chinese): https: scienceintegritydigest.files.wordpress.com 2020 07 stockphoto-papermill-july-2-2020-public-version-chn.pdf Your paper is listed in my coding system as  CF09 CF15 WH02 TW12 sWBother down1 St1a St2b St2d . Here is an explanation of which images it contained that were also found in other papers. Your paper used the following colony formation images: CF09  The W (used in 13 papers of the set), and CF15  Snowman (used in 15 papers). ![file] Next, your paper included wound healing photo WH02  The River , also illustrated above in comment #3. This image was used in 17 papers. Here are four examples: ![file] Then, there is transwell photo TW12  The Lion King , which I found in 7 different papers including yours. Here is a collage of some examples: ![file] Next, I spotted a negative correlation curve in your paper, which I called  Down1 , and which I found in a whopping 25 papers, including yours. Here are four examples of  Down1 : ![file] How do you explain that your paper contained images and plots used in so many other papers? If you claim that these images are really yours and that your lab did these experiments, how could the same images have ended up in dozens of other papers? I look forward to your reply. , 
 https: www.europeanreview.org article 22221 Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cCircular RNA circ-SMAD7 promoted ovarian cancer cell proliferation and metastasis by suppressing KLF6, by Y. Zhao, X.-P. Qin, Y.-P. Lang, D. Kou, Z.-W. Shao, published in Eur Rev Med Pharmacol Sci 2019; 23 (13): 5603-5610u2013DOI: 10.26355 eurrev_201907_18294u2013PMID: 31298312u201d has been withdrawn. The Publisher apologizes for any inconvenience this may cause. ,