Long noncoding RNA OR3A4 promotes cisplatin resistance of non-small cell lung cancer by upregulating CDK1
Authors: Y-D Xu
J Shang
M Li
Y-Y Zhang
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: lncRNAs; NSCLC; OR3A4; cisplatin resistance; RT-qPCR
Affiliations: Department of Central Sterile Supply, Yantai Yuhuangding Hospital, Yantai, China. .

Abstract:  Objective: Numerous studies have proved that long non-coding RNAs (lncRNAs) have an important role in malignant tumors, including non-small cell lung cancer (NSCLC). LncRNA olfactory receptor family 3 subfamily A member 4 (OR3A4) was explored to identify how it functions in resistance of NSCLC patients to cisplatin. Materials and methods: Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was utilized to detect OR3A4 expression in NSCLC patients. Then, we conducted Cell Counting Kit-8 (CCK-8) assay and flow cytometric analysis to detect the function of OR3A4 on the resistance of NSCLC cells to cisplatin. Furthermore, the potential mechanism was explored by mechanism assays. Results: Compared with OR3A4 expression of paired A549 cells, OR3A4 expression of A549/DDP cells was higher. Moreover, the functional assay showed that after OR3A4 was silenced in A549/DDP cells, cell cycle arrest and cell apoptosis was induced, and resistance to cisplatin was reversed. Furthermore, it was found that CDK1 expression was suppressed in A549/DDP cells by knockdown of OR3A4. Conclusions: The present work suggests that OR3A4 participates in regulating cell cycle, cell apoptosis of NSCLC cells and the resistance to cisplatin via upregulating CDK1, indicating that OR3A4 could be identified as a potential therapeutic target for NSCLC patients. 

Retracted: Yes

PubPeer comments:
 Figure 2C and 5C. * Red boxes: The A375 miR-326 mimics panel in Figure 2C appears to show an overlap with the A375 NC-siRNA panel in Figure 5C. ![file] , 
 Figs 2D, 5D, 6D.  The SK-MEL-28 and the A375 cells were transfected with miR-326 mimics or miR-NC. After a 48-h transfection, flow cytometric analysis was used to examine cell apoptosis ;  flow cytometry analysis were used to investigate the effects of KRAS knockdown by KRAS siRNA on melanoma cell ... apoptosis in vitro ;  Cell apoptosis was detected in the SK-MEL-28 and A375 cells transfected with miR-326 mimics with or without pcDNA3.1-KRAS . ![file] Figs 2D, 5D, 6D from [Zhao, Xu Zhang (2017)] .  Flow cytometric analysis was performed to examine the apoptosis of HCT-116 and CaCo-2 cells following transfection with miR-411 mimics or miR-NC. ;  Flow cytometric analysis was performed to assess the apoptosis of HCT-116 and CaCo-2 cells transfected with PIK3R3 siRNA or NC siRNA ;  apoptosis in HCT-116 and CaCo-2 cells transfected with miR-NC, miR-411 mimics or miR-411 mimics+pcDNA3.1-PIK3R3 were examined ... flow cytometric analysis . ![file] Could the authors comment on the presence of some panels in both papers? UPDATE: for orange-, dark blue-marked panels, see Figs 3B and 5D from [Shen et al (2018)] . ![] Bright green-marked panels: see Figs 2D, 4D, 5D of [Zhao et al (2018)] . ![] , 
 #2 Figs 2D, 5D, 6D again. Khaki and teal boxes added: ![file] To match panels in Figs 2D,5D,6E of [Wang et al (2019)] . ![file] -------------------------------- Violet panel and two turquoise panels mark matching panels in Figs 2D, 5D of [HU, Jin Wang (2018)] . ![] --------------------------------- Figs 2D, 5D, 6D again. dark green box added: ![] matching figs 2C,4C,5C of [Jia et al (2018)] . ![] ![] ,