MicroRNA‐183 induces epithelial‐mesenchymal transition and promotes endometrial cancer cell migration and invasion in by targeting CPEB1
Authors: Hui Chen
Ruichao Chen
Shaoyan Liu
Qingping Jiang
Qiongyan Lin
Hanzhen Xiong
Journal: Journal of Cellular Biochemistry (2018)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: CPEB1; apoptosis; endometrial cancer; epithelial-mesenchymal transition; invasion
Affiliations: Department of Pathology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, P.R. China., Department of Gynaecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, P.R. China.

Abstract:  The aim of this study is to evaluate the ability of microRNA-183 (miR-183) to influence epithelial-mesenchymal transition (EMT) and cell proliferation, migration, invasion, and apoptosis in endometrial cancer (EC) by targeting cytoplasmic polyadenylation element binding protein 1(CPEB1). EC tissues with matched nonmalignant tissues were collected from 208 EC patients. Ishikawa and RL95-2 cells were selected for cell experiments in vitro and each kind of cells were grouped into blank, negative control (NC), miR-183 mimic, miR-183 inhibitor, CPEB1 overexpression, and miR-183 mimic + CPEB1 overexpression groups. Expressions of miR-183, CPEB1, E-cadherin, and Vimentin were determined by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blotting. Cell viability, colony formation ability, migration, invasion, and apoptosis were assessed by MTT assay, clone formation assay, scratch test, Transwell assay, and flow cytometry. In vivo tumorigenesis of Ishikawa cells was evaluated by tumor formation in nude mice. The miR-183 expression was higher, but the CPEB1 expression was lower in EC tissues than in adjacent nonmalignant tissues. CPEB1 was confirmed as the target of miR-183 by dual-luciferase reporter assay. The miR-183 mimic group had increased cell viability, colony formation ability, cell invasion and migration, tumor volume and weight in nude mice, but decreased cell apoptosis when compared with the blank group. The expression of E-cadherin was down-regulate, but expression of Vimentin was up-regulate in the miR-183 mimic group in comparison with the blank group. In terms of a comparison between the blank group and CPEB1 overexpression group, the CPEB1 overexpression group had suppressed cell viability, colony formation ability, cell invasion and migration, tumor volume and weight, but increased cell apoptosis. The expression of E-cadherin was up-regulated, but the expression of Vimentin was down-regulated in the CPEB1 overexpression group in comparison with the blank group. The miR-183 mimic + CPEB1 overexpression group had higher miR-183 expression than the blank group. These findings indicate that miR-183 induces EMT, inhibits apoptosis, and promotes cell proliferation, migration, invasion, and in vivo tumorigenesis in EC by targeting CPEB1. 

Retracted: Yes

PubPeer comments:
 Fig 7:  Ishikawa and RL95-2 cell invasion and migration abilities in blank, NC, miR-183 mimic, miR-183 inhibitor, CPEB1, and miR183 mimics + CPEB1 groups for Ishikawa and RL95-2 cells, detected by the transwell assay I am not entirely convinced that the panels each illustrate different cell lines and conditions. ![file] I have left this overlap to consider separately, for it is particularly puzzling: cells are present in one version and not in the other. ![file] , 
 Fig 3A.  The transfection efficiency in blank, NC, miR-183 mimic, miR-183 inhibitor, CPEB1, and miR-183 mimics + CPEB1 groups, and the expression levels of miR-183 in each group for Ishikawa and RL95-2 cells, detected using RT-qPCR. A, the distribution of cells labeled with FAM fluorophore . One panel has an unexpected diagonal organisation, with similar cells repeating from upper right down towards lower left like a fading echo. ![file] , 
 #1 Fig 7A again. ![file] Many similarities with Fig 5F of [Xie et al (2017)] . ![file] More similarities with 5H from Xie et al. Also, similarities internal to Fig 5F H. ![file] , 
 Fig 7A AGAIN. Here below. At upper right, a composite constructed from three overlapping panels, rotated through 90-deg. Above, Fig 5 from [Liu et al (2017)] . Three panels appear to be details of that composite. ![file] Black, yellow, purple, green boxes mark other overlaps between panels of Fig 7A and 5. 90-deg rotations involved. , 
 Fig 7A again. ![file] Here marked up to emphasise overlaps with panels of Fig. 9 of [Yang et al (2015)] .  Transwell assay detecting cell invasion and metastasis in U2-OS cells. ![file] , 
 Another sighting! Fig 7A of [Xu et al (2016)] . H t Dendrodoa Grossularia. ![] , 
 Retracted [19 July 2021] . ---------------------------------------------- The above article, published online on 20 June 2018 in Wiley Online Library , has been retracted by agreement between the journal s Editor in Chief, Prof. Dr. Christian Behl, and Wiley Periodicals LLC. The retraction has been agreed following an investigation based on allegations raised by a third party. Several flaws and inconsistencies between results presented and experimental methods described were found. Thus, the editors consider the conclusions of this article to be invalid. ,