B7-H3 overexpression in pancreatic cancer promotes tumor progression
Authors: Xin-Guo Zhu
Feng Xiong
Guang-Bo Zhang
Zhi Li
Zi-Xiang Zhang
Xue-Guang Zhang
Hua Zhao
Wen-Juan Gan
Xin Zhao
De-Chun Li
Journal: International Journal of Molecular Medicine (2013)
Discipline: CLINICAL MEDICINE
Keywords: B7-H3; pancreatic cancer; RNA interference; cell migration; invasion
Affiliations: Department of General Surgery, The First Affiliated Hospital                     of Soochow University, Suzhou, P.R. China.

Abstract:  B7-H3, a member of the B7-family molecules, plays an important role in adaptive immune responses. In addition, B7-H3 is also expressed in several types of human cancers and is correlated with the poor outcome of cancer patients. However, its exact role in cancer is not known. In the present study, we compared B7-H3 expression in normal pancreas and pancreatic cancer tissue specimens, and determined the effects of low B7-H3 expression on the human pancreatic cancer cell line Patu8988 using lentivirus-mediated RNA interference. B7-H3 expression in pancreatic specimens was determined by enzyme-linked immunosorbent assay (ELISA). A Patu8988 cell line with low B7-H3 expression was established by lentivirus-mediated RNA interference to investigate the effect of B7-H3 on cell proliferation, migration and invasion in vitro. By establishing subcutaneous transplantation tumor and orthotopic transplantation pancreatic cancer mouse models, the effect of B7-H3 on cell proliferation, migration and invasion was studied in vivo. B7-H3 in tissue samples was significantly higher in the pancreatic cancer group than in the normal pancreas group (mean ± SD, 193.6±9.352 vs. 87.74±7.433 ng/g; P&lt;0.0001). B7-H3 knockdown by RNA interference decreased cell migration and Transwell invasion up to 50% in vitro. No apparent impact was observed on cell proliferation in vitro. In the subcutaneous transplantation tumor mouse model, the tumor growth rate was reduced by the knockdown of B7-H3. In the orthotopic transplantation pancreatic cancer mouse model, the effect of inhibiting metastasis by knocking down B7-H3 was assessed in terms of the average postmortem abdominal visceral metastatic tumor weight. This demonstrated that inhibition of B7-H3 expression reduced pancreatic cancer metastasis in vivo. In conclusion, B7-H3 is aberrantly expressed in pancreatic cancer. In addition to modulating tumor immunity, B7-H3 may have a novel role in regulating pancreatic tumor progression. 

Retracted: No

PubPeer comments:
 Fig 2E.  The promoted apoptosis of OC cells after PVT1 knockdown, as well as the FC diagram. ![file] Fig 4E.  Apoptosis of OC cells after transfection of miR-543 mimics, PVT1 and FC diagram . ![file] Each panel contains a halo of regularly-spaced dots, surrounding a central clump with a curious texture, almost woven in appearance. ![file] Perhaps these are instrumentation artefacts of some kind. Is it possible for the authors to upload the raw data files to a data repository somewhere? ------------------------------------- Here is a spreadsheet containing similar examples of these crocheted beetroot scatterplots (currently 11 retractions): https: docs.google.com spreadsheets d 1NKBplrpW1txuL9PjhYb3bbvtQbk-TcsnqyqI8Uj-5Q8 edit?usp=sharing ,