Geniposide alleviates lipopolysaccharide (LPS)-induced inflammation by downregulation of miR-27a in rat pancreatic acinar cell AR42J
Authors: Taishan Gao
Yanhua Wang
Xiaofen Zhang
Journal: Biological chemistry (2019)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: Geniposide; JNK; miR-27a; pancreatitis.
Affiliations: Department of Critical Care Medicine, Jining No. 1 People's Hospital, No. 6 Jiankang Road, Jining 272000, China., Affiliated Jining No. 1 People's Hospital of Jining Medical University, Jining Medical University, Jining 272000, China.

Abstract:  Pancreatitis is a disease caused by inflammation of pancreatic acinar cells. Geniposide (GEN) possesses anti-inflammation activities. Hence, we investigated the effects of GEN on lipopolysaccharide (LPS)-stimulated AR42J cells. AR42J cells were stimulated by LPS and then treated with GEN and/or transfected with miR-27a mimic or negative control. Cell viability and cell apoptosis were detected using the Cell Counting Kit-8 and flow cytometry, respectively. All related proteins were measured by Western blot. The expression of miR-27a was detected by quantitative real time-polymerase chain reaction (qRT-PCR). Moreover, the expression of inflammatory cytokines interleukin-6 (IL-6) and monocyte chemoattractant protein (MCP)-1 was analyzed by qRT-PCR and Western blot. LPS significantly decreased cell viability, and enhanced cell apoptosis and IL-6, MCP-1 expression. Then GEN administration alleviated inflammatory injury by increasing cell viability, while reducing apoptosis, and IL-6 and MCP-1 expression. GEN downregulated miR-27a expression which was induced by LPS. Transfection with miR-27a mimic partially eliminated the protective effects of GEN. The phosphorylation of JNK and c-Jun was downregulated by GEN while upregulated by miR-27a overexpression. GEN alleviates LPS-induced AR42J cell injury as evidenced by promoting cell growth, and upregulation of IL-6 and MCP-1. This process might be modulated by down-regulating miR-27a and inactivation of JNK pathway. 

Retracted: No

PubPeer comments:
 Figure 1C. Much more similar than you would expect. ![file] , 
 Much more similar than you would expect. ![file] , 
 Figures 4A and 5C. Much more similar than you would expect. ![file] , 
 Figure 7A. Unexpected symmetry. ![file] , 
 Indeed it seems that part of the blot has been  recycled , probably for convenient reasons when assembling an image for the manuscript submitted for publication. Please note that the issue concerns a control of a single experiment representing several experiments with similar results. Therefore the main outcome shown in this figure cannot be reconsidered. , 
 #2 Indeed it seems that a blot has been  recycled . I apologize for this. Please note that the issue concerns a control of a single experiment representing several experiments with similar results. Therefore the main outcome shown in this figure cannot be reconsidered. , 
 #1 Indeed it seems that part of the blot has been  recycled , probably for convenient reasons when assembling an image for the manuscript submitted for publication. Please note that the issue concerns a control of a single experiment representing several experiments with similar results. Therefore the main outcome shown in this figure cannot be reconsidered , 
 #3 There also it seems that a blot for controling gel loading has been recycled when assembling this figure. I apologize for this. Please note that this does not change the main outcome depicted in this figure, as these experiments have been reproduced several times, and thoroughly controled each time, even though we should not have assembled this figure the way it was done. , 
 #4 Indeed there may be an issue with these two lanes, which represent a control for gel loading. Again, this experiment is representative of several assays showing identical results and therefore the major outcome of this figure cannot be reconsidered. ,