Structural determinants of schisandrin B which enhance mitochondrial functional ability and glutathione status as well as heat shock protein expression in rat hearts and H9c2 cells
Authors: Po Yee Chiu
Kam Ming Ko
Journal: Molecular and Cellular Biochemistry (2005)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: ex vivo model; isolated-perfused rat hearts; cultured H9c2 cells; schisandrin B; schisandrin A
Affiliations: Department of Biochemistry, The Hong Kong University of Science & Technology, Clear Water Bay, Hong Kong, China. 

Abstract:  Using an ex vivo model of isolated-perfused rat hearts and cultured H9c2 cells, the structure-activity relationships of schisandrin B (Sch B), and analogs lacking either the methylendioxy group or cyclooctadiene ring, schisandrin A (Sch A) and dimethyl diphenyl bicarboxylate (DDB), respectively, were investigated. Pretreatment with Sch B, but not with Sch A or DDB, protected against myocardial ischemia-reperfusion (I-R) injury in rats. Although Sch B pretreatment largely prevented H9c2 cells from menadione-induced cytotoxicity, Sch A pretreatment produced only a marginal protection. However, DDB pretreatment did not cause any detectable effect. The myocardial and cellular protection afforded by Sch B pretreatment correlated with increases in mitochondrial ATP generation capacity and/or reduced glutathione level as well as heat shock protein (Hsp)25/70 expression, under both control and oxidative stress conditions. The results indicate that the methylenedioxy group and the cyclooctadiene ring are important structural determinants of Sch B in enhancing mitochondrial functional ability and glutathione status, as well as tissue Hsp 25/70 expression, thereby protecting the myocardium against I-R injury. 

Retracted: No

PubPeer comments:
 The Western immunoblots in Figure 3 look artificial, without any background noise and halo around the crystal clear bands. The microscopy data shown in Figure 5 and 6 also have an unusual pattern. Squares containing images of cells seem to be put together. The space around is completely white. ![file] , 
 2021 retraction. https: content.iospress.com articles cancer-biomarkers cbm210951 IOS Press has retracted the following publication from its online content: [Cancer Biomarkers, 22(1) (2018), 111u2013118] 10.3233 CBM-171045Serum miR-658 induces metastasis of gastric cancer by activating PAX3-MET pathway: A population-based studyYuanyu Wua, Xiaoyu Wanb, Fujian Jia, Zheyu Songa and Xuedong FangaaDepartment of Gastrointestinal, Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin, ChinabDepartment of Breast and Thyroid Surgery, The Second Hospital of Jilin University, Changchun, Jilin, China The above article, originally published in Cancer Biomarkers volume 22, number 1, 2018, pp. 111u2013118, has been retracted from its online catalogue in consultation between the corresponding author and the publisher of Cancer Biomarkers. The published paper mainly focuses on Serum miR-658 inducing metastasis of gastric cancer. However, recent work showed that serum miR-658 is unstable with quick turnover even at a low temperature. The work of this study cannot be repeated. In this light the journal cannot condone publication of this paper and has decided to retract it from its online catalogue. ,