Dihydroartemisinin inhibits TCTP-dependent metastasis in gallbladder cancer
Authors: Wei Gong
Lin Jiang
Shanshan Xiang
Haibin Liang
Zihang Xu
Zheng Wang
Fei Zhang
Yunping Hu
Yingbin Liu
Xuefeng Wang
Huaifeng Li
Yong Zhang
Yijian Zhang
Yuanyuan Ye
Qiang Ma
Journal: Journal of Experimental & Clinical Cancer Research (2017)
Discipline: CLINICAL MEDICINE
Keywords: Dihydroartemisinin; Gallbladder cancer; Invasion; Metastasis; TCTP.
Affiliations: Department of General Surgery, Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Room 517, Building 22, Xinhua Hospital, 1665 Kongjiang Rd., Shanghai, 200092, China., Shanghai Research Center of Biliary Tract Disease, 1665 Kongjiang Road, Shanghai, 200092, China., Laboratory of Integrative Medicine, School of Basic Medical Sciences, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai, 201203, China., Department of General Surgery, Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Room 517, Building 22, Xinhua Hospital, 1665 Kongjiang Rd., Shanghai, 200092, China. ., Shanghai Research Center of Biliary Tract Disease, 1665 Kongjiang Road, Shanghai, 200092, China. .

Abstract:  Background: Patients with metastatic or relapsed gallbladder cancer generally have a poor prognosis. Therefore, targeting metastasis is one arm of therapeutic strategies to treat gallbladder cancer. Methods: Levels of translationally controlled tumor protein (TCTP) were measured in samples of gallbladder cancer by immunohistochemical staining. Wound healing, migration and invasion assays were used to investigate the motility of cells. Western blot assay was used to investigate the levels of TCTP and other proteins. Liver metastasis models and lung metastasis models were established to investigate the inhibitory effect of Dihydroartemisinin on gallbladder cancer metastasis. Results: TCTP is aberrantly expressed in gallbladder cancer patients and associated with metastasis and a poor prognosis. Depleting TCTP significantly inhibited gallbladder cancer cell migration and invasion. We found that Dihydroartemisinin as a potent inhibitor of TCTP inhibited TCTP-dependent cell migration and invasion by reducing cell division control protein 42 homolog (Cdc42) activation. In addition, in mice with xenografted tumors, treatment with Dihydroartemisinin decreased gallbladder cancer cell metastases and improved survival. Conclusions: These findings provide new insights into the therapeutic activity of Dihydroartemisinin as a treatment for gallbladder cancer metastasis. 

Retracted: No

PubPeer comments:
 Figure 3A appears to include an image overlap; see red boxes. *** ![file] , 
 Correction to: J Exp Clin Cancer Res http: dx.doi.org 10.1186 s13046-017-0531-3 In the original publication of this article [1], there are mistakes in Fig. 3a and Fig. 3d. ![file] , 
 Figures 2D and 3A appear to have any image which overlap. I donu2019t believe this was addressed in the correction. *** ![file] , 
 Dear Dr. Biskrensis, I got you message. Thank you very much for your comments on our paper. I will take a look at our original data regarding the figure panels and respond to you as soon as possible. Many thanks again. Best regards Yingbin , 
 2nd Correction Published: 04 April 2022  Following publication of the original article [1], the authors (sic) identified minor errors in Figs. 3, 6, and S1; specifically: Fig. 3A: Incorrect migration assay images originally used for the TCTP-positive cell lines GBC-SD; the correct images are now used, and a scale bar has been added Fig. 6G: scale bars have been added to the images showing lung metastatic tissues of gallbladder cancer perfused with Indian ink Fig. S1F: scale bars have been added to the images showing lung metastatic tissues of gallbladder cancer perfused with Indian ink The corrected figures are given here. The correction does not have any effect on the final conclusions of the paper. ![file] , 
 Dear Dr. Biskrensis, thank you very much for your comments. The inadvertent errors have been corrected. We apologize for these errors and for any inconvenience that they may have caused. ,