A New Zn(II) Complex: Fluorescent Detection of Fe3+ Ions in Water and Prevention Effect on DVT By Regulating Platelets Numbers and Activity of Clotting Factor
Authors: Yue-Ying Pei
Hong-Yuan Xue
Jie Gao
Xin Liu
Journal: Journal of Fluorescence (2020)
Discipline: CHEMISTRY
Keywords: DVT; Fe3+ sensor; Imidazole-carboxyl ligand; Molecular docking; Porous framework
Affiliations: Department of Ultrasonography, Hebei General Hospital, Shijiazhuang, Hebei, China., Department of Ultrasonography, Hebei General Hospital, Shijiazhuang, Hebei, China. .

Abstract:  [Zn(dima)(H2O)0.5]·H2O (1), a three-dimensional metal organic framework (MOF) with high porosity was formed by self-assembly of 4,6-di(1 h-imidazol-1-yl)isophthalic acid (H2dima) and Zn2+ ion. Owning to its excellent luminescence and excellent water stability, complex 1 can be used as a super sensitive sensor to detect Fe3+ ions via the behaviors of fluorescence quenching. At the same time, the mechanism for the fluorescence quenching is also further discussed. Furthermore, the prevention effect of the compound on the deep vein thrombosis of the lower extremities (DVT) after lower venous CHIVA surgery was evaluated in vivo. Firstly, the DVT animal models was constructed and the number of platelets was measured with flow cytometry and content of clotting factor IX and anticoagulant factor III was also detected with Lowry method after compound treatment. The molecular docking simulation results indicate that the Zn(II) complex has activity to protein docking pocket with different sizes. 

Retracted: No

PubPeer comments:
 The opening paragraph is incoherent, but the random meaningless References do not help.  Deep vein thrombosis (DVT) of lower extremities is an abnormal thrombosis of lower extremities in deep veins, resulting in strong venous obstruction, causing blood reflux disorders [1]. - [1]. Kashtan HW, Simon MA, Beyer CA, Wishy A, Hoareau GL, Grayson JK, Johnson MA (2020) Effects of extended lower extremity cooling following zone 3 REBOA in a porcine hemorrhage model. Mil Med 185:42u201349  Lower venous CHIVA surgery has a long course of disease, which increases the number of platelets, the content of clotting factors and decreases the activity of anticoagulant factors [2]. - [2]. Ciudad P, Vargas MI, Bustamante A, Agko M, Manrique OJ, Ludeu00f1a J, Castro J, Wiegering G, Navarro W, Reynaga C (2020) Combined radical reduction with preservation of perforators and distal lymphaticovenular anastomosis for advanced lower extremity lymphedema. Microsurgery 40:417u2013418  In addition, the long-term bed rest of the patients causes the decrease of muscle contraction, which leads to a slowing of the blood flow velocity in the vein, an increase in blood viscosity, resulting in abnormal blood vessel condensation and thrombosis, and the formation of deep vein thrombosis of the lower extremity, which may cause pulmonary embolism, seriously affect the quality of life of patients, and even die in respiratory failure [3, 4]. - [3]. Bond CW, Dorman JC, MacFadden LN, Munce TA (2020) Dynamic postural stability in active, adolescent males following repeated bouts of aerobic exercise in hot and temperate environments: a pilot study. Mil Med 185:368u2013375 - [4]. McFadden C, Daniels K, Strike S (2020) The sensitivity of joint kinematics and kinetics to marker placement during a change of direction task. J Biomech 101:109635 Perhaps things would make a little more sense if the authors had *described* CHIVA surgery (instead of mentioning it as a meaningless buzzword) but I am skeptical. The crucial point of the treatment is that *no bed rest* is involved. Could the authors explain the relevance of [6]?  Nevertheless, excessive Fe3+ will do harm to proteins and nucleic acids, due to Fe3+ can catalyze Fenton reaction to produce active oxygen [6]. - [6]. Zhang D, Wang MM, Jiang N, Liu Y, Yu XN, Zhang HB (2020) Electrochemical corrosion behavior of Ni-doped ZnO thin film coated on low carbon steel substrate in 3.5% NaCl solution. Int J Electrochem Sci 15:4117u20134126 How about the relevance of [7-9]? Thus, it is of important sense for environment and health of human beings to develop robust, reliable and efficient Fe3+ detection approaches [7u20139]. - [7]. Kuang Y, Zhao J, Gao Y, Lu C, Luo S, Sun Y, Zhang D (2020) Enhanced hydrogen production from food waste dark fermentation by potassium ferrate pretreatment. Environ Sci Pollut R 27:18145u2013 18156 - [8]. Feng X, Feng YQ, Liu L, Wang LY, Song HL, Ng SW (2013) A series of Zn-4f heterometallic coordination polymers and a zinc complex containing a flexible mixed donor dicarboxylate ligand. Dalton T 42:7741u20137754 - [9]. Du H, Ma C, Ma W, Wang H (2018) Microstructure evolution and dielectric properties of Ce-doped SrBi4Ti4O15 ceramics synthesized via glycine-nitrate process. Process Appl Ceram 12:303u2013312 There must be *some* relevance, *somewhere*, if we keep looking.  In recent years, it has been shown that bifunctional ligands are ideal for the preparation of photoluminescence MOFs [19, 20]. - [19]. Chen DM, Zhang XJ (2019) Stepwise and hysteretic sorption of CO2 in polycatenated metal-organic frameworks. CrystEngComm 21:4696 , 
 This paper has further oddities regarding the DVT experiments, which are not sufficiently described to be interpreted. There is no indication what samples were scored for platelet content, and the graphs in Figure 6 appear to be meaningless with regards to any known DVT model. In a procoagulant situation, platelet numbers decrease as they are consumed in clots. There is no mechanism that can increase platelet count like this. ![file] There is a similar apparent confusion in the claimed effects of treatment on coagulation factors, where the authors claim to have measured FIX and  anticoagulant factor III (which does not exist as far as I could determine) using the Lowry method - which of course simply measures total protein in a sample whereas here a specific assay is implied. ![file] It is difficult to see how these results support the authors claims. ,