miR-365 functions as a tumor suppressor by directly targeting CYR61 in osteosarcoma
Authors: Yan Zhou
Yawei Xu
Haijiao Chu
Changying Dong
Junling Wang
Rui Yin
Journal: Biomedicine & Pharmacotherapy (2018)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: CYR61; Invasion; Osteosarcoma; Proliferation; miR-365.
Affiliations: College of Bioengineering, Jilin Agricultural Science and Technology University, Jilin City 132101, PR China., College of Bioengineering, Jilin Agricultural Science and Technology University, Jilin City 132101, PR China. Electronic address: .

Abstract:  Increasing evidence indicates that microRNAs(miRNAs) are often aberrantly expressed in osteosarcoma (OS) and play critical roles in OS tumorigenesis. Therefore, the discovery of miRNAs may provide a new and powerful tool for understanding the mechanismof OS initiation and development. The aim of this study was to investigate the functional significance of miR-365 and identify its possible mechanism in OS cells. Here, wefound that the expression level of miR-365 is significantly downregulated in OS tissues and cell lines, and its expression isassociated with the clinical stage, distant metastasis, tumor grade, and poor overall survival rate. The overexpression of miR-365 is able to inhibit cell proliferation, migration, and invasion in Saos-2 and MG-63 cells. Moreover, the cysteine-rich angiogenic inducer 61 (CYR61) has been identified as a target of miR-365 in OS cells, and its expression is found to be significantly increased in OS tissues, which is negatively correlated with miR-365. Furthermore, CYR61 overexpression significantly attenuated the suppressive effects of miR-365 on the proliferation, migration, and invasion of Saos-2 and MG-63 cells. Therefore, we consider that miR-365 acts as a tumor suppressor in OS, partly, by targeting CYR61 expression. 

Retracted: No

PubPeer comments:
 Fig 3.  miR-365 inhibits cell migration and invasion in OS cells. (A,B) Cell migration (A) and invasion (B) were determined in U2OS and MG-63 cells transfected with miR-365 mimic or miR-NC by transwell assay, respectively . ![file] A panel appears twice, acquiring an additional group of cells in one of its manifestations. , 
 *[left]* Fig 5B.  Western blot analysis of CYR61 protein levels in U2OS and MG63 cells transfected with an miR-365 mimic with or without a pCDNA3.1-CYR61. *[right]* Fig 5A from [Lin et al (2017)] .  Western blot and analysis of AKT3 protein expression in TPC-1 and SW1736 cells expressing miR-217 or miR-217 plus AKT3 overexpression plasmid . ![file] Unexpected similarities. , 
 *[right]* Fig 4E.  expression levels in U2OS and MG63 cells transfected with miR-365 mimic or miR-NC was detected by ... Western blot. *[left]* Fig 5B from [Fei et al (2018)] .  qRTPCR analysis of PTEN mRNA expression in U2OS cells transfected with miR-18a-5p mimic miR-NC or pFER1l4 plasmid pcDNA3.1 plasmid. ![file] , 
 #1 Another issue with Fig 3 is that the repeated manipulated panel has appeared in papers elsewhere. ![file] Fig 6 from  Downregulation of long non-coding RNA ANRIL suppresses lymphangiogenesis and lymphatic metastasis in colorectal cancer ([Sun et al 2016] ): ![] Fig 6 from  Effects of RUNX3 mediated Notch signaling pathway on biological characteristics of colorectal cancer cells ([Li et al 2017] ): ![file] , 
 #2 Hello, is that the Department of Unexpected Similarities? I have a concern. - *[left]* Fig 5B, and Fig 5A from [Lin et al (2017)] . - *[right]* Fig 3 from [Zhu et al (2016)] . ![file] ,