Andrographolide inhibits intimal hyperplasia in a rat model of autogenous vein grafts
Authors: Wei-Xin Meng
Xue-Song Jiang
Hong-Yu Liu
Bai-Chun Wang
Ye Tian
Zhi-Tao Zhu
Journal: Cell Biochemistry and Biophysics (2011)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: saphenous vein; coronary artery bypass grafting; intimal hyperplasia; andrographolide; rat veins
Affiliations: Department of Cardiovascular Surgery, First Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, China.

Abstract:  The saphenous vein is considered a common conduit for coronary artery bypass grafting. A major limitation involved is the high graft occlusion rate. We evaluated the effect and mechanism of andrographolide on intimal hyperplasia (IH) of autografted rat veins. For this purpose, 140 female Sprague-Dawley rats were randomly divided into experimental (andro) and control groups. Andro rats received andrographolide (200 mg/kg) lavaged once daily for 2 days before surgery while controls received normal saline. The external jugular vein was grafted into the ipsilateral carotid artery. The animals were killed at 1/3 days and 1/2/4/6/8 weeks postoperatively. The neointima to media area (I/M) ratio was determined. Expression of the p65, E-selectin and MMP-9 proteins/mRNA was also determined. Autografted rat veins displayed IH postoperatively. In andro rats, compared with controls, IH was significantly reduced (P &lt; 0.01) at 2/4/6/8 weeks but not at 1/3/7 days postoperatively. Andrographolide also significantly (P &lt; 0.05) reduced the expression of E-selectin and MMP-9 proteins/mRNAs at 2/4/6/8 weeks postoperatively whereas p65 protein/mRNA was significantly (P &lt; 0.05) diminished at 1/3 days and 1/2/4/6/8 weeks postoperatively as compared with controls. Therefore, it was concluded that andrographolide inhibited IH in autografted rat veins through the suppression of p65, E-selectin, and MMP-9 at the transcriptional level. 

Retracted: No

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 I see the similarities too in Figure 1B, as marked in #1, and some additional regions. Could the authors or the institute maybe post the original blots to take away any of our concerns? ![file] In addition, could someone please clarify why the Investigation mentioned in #4 was conducted at the Wellcome Trust in London? The authors are all affiliated with the University of Toronto, and the research was supported by grants from the Canadian Institute of Health Research (CIHR) and the Parkinson Society of Canada. In addition, the paper states that Postdoctoral support was provided by the Leon Frederick Foundation and the Journal of Cell Science travel grant . The Leon Frederick Foundation is not easy to find online; I can only find mentions of it as funding statement in some papers. , 
 Figure 7A might also show some repetitive elements. Could the authors please provide the original blots? ![file] , 
 August September 2020 retraction: https: www.jbc.org content 295 39 13694.short  *This article has been withdrawn by Sabine Wislet-Gendebien, Cheryl D Souza, Toshitaka Kawarai, Peter St George-Hyslop, David Westaway, Paul Fraser, and Anurag Tandon. The u03b1-synuclein and GAPDH bands at 30 min were superimposed on the earlier time points in Fig. 1B. The GAPDH bands in Fig. 4B were reused in Fig. 7A.* , 
 2020 retraction. https: www.jbc.org content 295 39 13694.short VOLUME 281 (2006) PAGES 32148u201332155 This article has been withdrawn by Sabine Wislet-Gendebien, Cheryl D Souza, Toshitaka Kawarai, Peter St George-Hyslop, David Westaway, Paul Fraser, and Anurag Tandon. The u03b1-synuclein and GAPDH bands at 30 min were superimposed on the earlier time points in Fig. 1B. The GAPDH bands in Fig. 4B were reused in Fig. 7A. , 
 https: retractionwatch.com 2020 09 30 author-says-misguided-efforts-for-the-ideal-western-blot-led-to-the-withdrawal-of-these-studies ,