MicroRNA-299-3p regulates proliferation, migration and invasion of human ovarian cancer cells by modulating the expression of OCT4
Authors: Ruiyan Zhao
Chunxiang Lou
Qiyong Liu
Journal: Archives of Biochemistry and Biophysics (2018)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: Cell migration; Ovarian cancer: OCT-4; Proliferation; miR-299-3p.
Affiliations: Department of Obstetrics and Gynecology, The Third People's Hospital of Jinan, Jinan, Shandong 250132, China. Electronic address: ., Department of Cardiology, The Third People's Hospital of Jinan, Jinan, Shandong 250132, China., Department of Obstetrics and Gynecology, The Third People's Hospital of Jinan, Jinan, Shandong 250132, China.

Abstract:  Ovarian cancer is among the most prevalent and lethal types of cancers in women. Several factors such as late diagnosis, unavailability of the reliable biomarkers, frequent relapses and dearth of efficient therapeutic targets form bottleneck in the treatment of ovarian cancers. In this study we investigated the potential of less studied miR-299-3p as the therapeutic target for the treatment of ovarian cancer. The results of the present investigation revealed that miR-299 is significantly upregulated in the ovarian cancers and suppression of its expression inhibits the proliferation by induction of apoptosis as well suppresses migration and invasion of the SKOV3 cancers cells. Further, OCT-4 was found to be putative target of miR-99-3p in ovarian cancer and inhibition of OCT-4 had similar effects as that of miR-299 inhibition on cell migration and invasion. Intriguingly, even overexpression of miR-299-3p could not rescue the effects of OCT-4 suppression on SKOV3 cell proliferation, migration and invasion. On contrary, overexpression of OCT-4 in SKOV3 cells transfected with miR-299-3p transfected could nullify the effects of miR-200-3p on proliferation, migration and invasion of the SKOV3 cells. Taken together, miR-299-3p regulated cell proliferation and metastasis by modulating the expression of OCT-4 and as such may prove to be an important therapeutic target. 

Retracted: No

PubPeer comments:
 *[left]* Fig. 3B from [Zhao, Liu Lou (2018)] .  Effect of miR-299-3p inhibition on ... (B) cell invasion of SKOV3 ovarian cancer cells . *[right]* Figure 6B from [Zhou Hu (2018)] .  cell invasion of U2OS cells treated with Evodiamine at IC50 concentration . ![file] , 
 *[above]* Fig 2B. ImageTwin informs me that the panels were obtained from *[below]* Fig 1D of  RNA-binding protein RBM3 prevents NO-induced apoptosis in human neuroblastoma cells by modulating p38 signaling and miR-143 ([Yang et al 2017] ). ![file] , 
 *[left]* Fig 3A. The panels are taken from *[right]* Fig 5A of  Loss of CAR promotes migration and proliferation of HaCaT cells, and accelerates wound healing in rats via Src-p38 MAPK pathway ([Su et al 2016] ). ![file] , 
 #1 But wait, there s more! - *[above]*, Figs 3B and 5C. - *[below]* Figs 2F G, 4G H from  Long non-coding RNA SPRY4-IT1 promotes development of hepatic cellular carcinoma by interacting with ERRu03b1 and predicts poor prognosis ([Yu et al 2017] ). ![file] Fig 4 from  Long nonu2011coding RNA LINC00525 regulates the proliferation and epithelial to mesenchymal transition of human glioma cells by sponging miRu2011338u20113p ([Wan et al 2020] ). ![file] ,