miR-365 targets ADAM10 and suppresses the cell growth and metastasis of hepatocellular carcinoma
Authors: Kai Liu
Songyang Liu
Yingchao Wang
Wei Zhang
Bai Ji
Yahui Liu
Journal: Oncology Reports (2017)
Discipline: CLINICAL MEDICINE
Keywords: miR-365; hepatocellular carcinoma; HCC; tumor progression; tumor-suppressor
Affiliations: Department of Hepatobiliary and Pancreatic Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.

Abstract:  Expression of miR-365 has been reported to be downregulated in hepatocellular carcinoma (HCC). However, the biological function and underlying mechanism of miR-365 in HCC growth and metastasis remain unclear. The aim of the present study was to explore the role of miR-365 in HCC progression. We found that miR-365 expression was downregulated in HCC tissues and cell lines. Further results showed that low expression of miR-365 was significantly associated with tumor-node-metastasis (TNM) stage and lymph node metastasis. Functional assays revealed that overexpression of miR-365 significantly inhibited cell proliferation, colony formation, migration and invasion of HCC cells in vitro, and suppressed tumor growth in vivo. Mechanistic investigations demonstrated that ADAM10 (a disintegrin and metalloproteinase 10) is a target of miR-365 in HCC. In addition, knockdown of ADAM10 in HepG2 cells significantly inhibited cell proliferation, colony formation, migration and invasion, which mimicked the suppressive effects induced by miR-365 overexpression. Restoration of ADAM10 expression partially reversed the suppressive effects mediated by miR-365 overexpression. Taken together, these results indicate that miR-365 functions as a tumor-suppressor in HCC through targeting ADAM10, and may serve as a promising candidate for therapeutic applications in HCC treatment. 

Retracted: No

PubPeer comments:
 *[left]* Fig 2E.  miR-365 inhibits HCC growth in vitro and in vivo.... (E) Images of the tumor tissue . *[right]* Fig 7B from [Li et al (2017)] .  Representative images of tumors treated with miR-592 mimic or miR-Ctrl. ![file] Could the authors comment on the appearance of three tumors in both compositions? , 
 Fig 2C.  colony formation ... determined in HepG2 cells transfected with miR-365 mimic or miR-NC. ![file] For comparison: *[left]* Fig 5C from [Li et al (2017a)] .  colony formation ... detected in SCC9 cells transfected with miR-NC or and miR-152 mimic with without c-MET overexpression plasmid . *[right]* Fig 5C from [Li et al (2017b)] .  colony formation ... determined in SKOV3 cells transfected with miR-218 mimic or miR-NC with or without RUNX2 overexpression plasmid . ![] Blue circles call attention to an unexpected similarity. , 
 Retracted [12 September 2022] .  Following the publication of this paper, it was drawn to the Editors attention by a concerned reader that certain of the colony formation assay data shown in Fig. 2C and the tumor images shown in Fig. 2E were similar to data appearing in different form in other articles by different authors. Owing to the fact that the contentious data in the above article had already been published elsewhere, or were already under consideration for publication, prior to its submission to Oncology Reports, the Editor has decided that this paper should be retracted from the Journal. After having been in contact with the authors, they agreed with the decision to retract the paper. The Editor apologizes to the readership for any inconvenience caused. ,