Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154
Authors: Rongyuan Zhang
Chuan Lv
Jianquan Hou
Baocai Xu
Weijie Zhang
Guangbo Zhang
Journal: Artificial Cells Nanomedicine and Biotechnology (2020)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: LINC01939; Renal carcinoma; miR-154; migration; proliferation.
Affiliations: Department of Urology, The First Affiliated Hospital of Soochow University, Jiangsu, China, Department of Urology, Jining No, 1 People’s Hospital, Jining, China, Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Jiangsu, China, Jiangsu Key Laboratory of Clinical Immunology, Soochow University, Jiangsu, China, Jiangsu Key Laboratory of Gastrointestinal Tumor Immunology, Jiangsu, China

Abstract:  Renal carcinoma (RCC) is widely accepted as a malignant tumour of urinary system. Long intergenic non-coding RNA 1939 (LINC01939) is a novel lncRNA which was found to be down-regulated in RCC. Thus, we set out to explore the effect and regulation mechanism of LINC01939 in RCC. LINC01939 and miR-154 in RCC tissues and cell lines were detected using qRT-PCR assay. To examine cellular viability of ACHN and CAKI-1 cells, cell counting kit-8 (CCK-8) assay was exploited here. Flow cytometric analysis was conducted to examine apoptosis. Cell mobility was valued through wound healing assays. Western blotting was applied for examination of proteins related to proliferation, apoptosis, migration and Wnt/β-catenin/Notch. LINC01939 was down-regulated in RCC tissues. LINC01939 overexpression impeded proliferation and migration, and induced apoptosis. Further study found that the overexpression of LINC01939 strongly suppressed miR-154 expression. Then, the inhibiting effect of overexpressed LINC01939 on proliferation and mobility and the promoting role of LINC01939 in apoptosis were abolished by the combination of miR-154 mimic. Finally, we found that overexpressed LINC01939 inactivated Wnt/β-catenin and Notch through suppressing miR-154. Up-regulation of LINC01939 inhibited proliferation and migration of RCC cells by down-regulating miR-154. 

Retracted: No

PubPeer comments:
 The Western Blots in this paper fit a tadpole template reported in a number of places, including Elisabeth Bik s description. https: scienceintegritydigest.com 2020 02 21 the-tadpole-paper-mill The 400+ cases noticed so far are [tabulated in a spreadsheet] . They are notable for the similar conformation of protein blobs in every band, and the appearance of the same *background* across bands. Here, Fig 5: ![file] There is no obvious connection between the name and the email address of the second corresponding author. ![file] , 
 Expression of Concern [6 April 2021] . ------------------------------- We, the Publisher and Editor of Artificial Cells, Nanomedicine, and Biotechnology, are issuing an Expression of Concern for the following article: Rongyuan Zhang, Weijie Zhang, Baocai Xu, Chuan Lv, Jianquan Hou Guangbo Zhang (2020) Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154. Artificial Cells, Nanomedicine, and Biotechnology, 48(1), 695u2013702, DOI: 10.1080 21691401.2020.1725024 After publication of this article, questions about the scientific integrity of the article content were brought to the Publisher and Editoru2019s attention. We reached out to the authors requesting that they supply information that would confirm the articleu2019s integrity. The authors provided several files in response to our queries, but they were unable to provide the original data in a format which satisfies our requirements to confirm the authenticity of some types of data. In particular, they were unable to provide full western blots, where the molecular weight markers are indicated. Therefore, as we continue to work through the issues raised, we advise readers to interpret the information presented in the article with due caution. The authors have been notified about this Expression of Concern. ,