B4GALT3 up-regulation by miR-27a contributes to the oncogenic activity in human cervical cancer cells
Authors: Min Liu
Hua Tang
Yanrui Sun
Xi Yang
Journal: Cancer Letters (2016)
Discipline: CLINICAL MEDICINE
Keywords: B4GALT3; Cervical cancer cells; EMT; miR-27a; β1-integrin.
Affiliations: Tianjin Life Science Research Center and School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China., Tianjin Life Science Research Center and School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China. Electronic address: .

Abstract:  β-1,4-Galactosyltransferase III (B4GALT3) is an enzyme responsible for the generation of poly-N-acetyllactosamine and is involved in tumorigenesis. However, B4GALT3-dysregulation and its role in cervical cancer cells are unknown. Herein, we found that B4GALT3 was upregulated in cervical cancer tissues compared to adjacent non-tumor tissues. B4GALT3-overexpression promoted, whereas B4GALT3-knockdown suppressed the cellular migration, invasion and EMT of HeLa and C33A cervical cancer cells. To explore the mechanism of dysregulation, B4GALT3 was predicted to be a target of miR-27a. EGFP and pGL3-promoter reporter assay showed miR-27a binds to B4GALT3 3'UTR region but enhanced its expression. RT-qPCR showed miR-27a was also upregulated and presented positive correlation with B4GALT3-expression in cervical cancer tissues. miR-27a-overexpression promoted, but blocking-miR-27a repressed these malignancies in HeLa and C33A cells. Furthermore, shR-B4GALT3 counteracted the promotion of malignancies induced by miR-27a, suggesting miR-27a upregulates B4GALT3 to enhance tumorigenic activities. In addition, we found that B4GALT3 significantly enhances β1-integrin stability, thus mediating promotion of B4GALT3 on malignancy in cervical cancer cells. Altogether, our findings evidenced that B4GALT3 upregulated by miR-27a contributes to the tumorigenic activities by β1-integrin pathway and might provide potential biomarkers for cervical cancer. 

Retracted: No

PubPeer comments:
 Figure 4B this time. *** ![file] *** ![file] , 
 November 2020 corrigendum, found here: https: www.sciencedirect.com science article pii S030438352030375X It reads:  The authors regret that u201cThe images of the wound healing in Fig. 4B, 1C and 5A were inadvertently wrongly placed by mistake during the figure preparation of manuscript. In Fig. 4B, the image of ASO-miR-27a at 0 h in HeLa was misplaced by mistake. In Fig. 1C, the image of pCD3-Flag at 0 h and image of pSilencer-NC at 48 h in HeLa were misplaced by mistake. In Fig. 5A, images of pcDNA3+pSilencer-NC and pri-miR-27a + pSilencer-NC at 0 h in C33A were misplaced. The incorrect images are revised. The corrections do not change the results and conclusions of the article. The updated figures are as follow.u201d The authors sincerely apologise for any inconvenience caused due to our carelessness. I have reconstructed the five duplications addressed in the correction in this collage of the original panels, highlighted with boxes of the same color. The duplication marked in comment #1 by Actinopolyspora Biskrensis is shown in red. ![file] , 
 Could the authors also please take a look at the transwell panels in Figures 1D, 4A, and 5B? Some possible duplications might be visible in those panels as well. ![file] , 
 Could the authors also please take a look at the phase contrast microscopy panels in Figures 1E, 4C, and 5C? Some possible duplications might be visible in those panels as well. ![file] ,