MiR-126 Regulates the ERK Pathway via Targeting KRAS to Inhibit the Glioma Cell Proliferation and Invasion
Authors: Chengyuan Ma
Pengfei Ge
Yang Li
Yunqian Li
Journal: Molecular Neurobiology (2017)
Discipline: NEUROSCIENCE & BEHAVIOR
Keywords: ERK pathway; Glioma; Invasion; KRAS; Proliferation
Affiliations: Department of Respiratory Medicine, The First Hospital of Jilin University, Changchun, 130021, Jilin Province, China., Department of Neurosurgery, The First Hospital of Jilin University, Xinmin Street 71, Changchun, 130021, Jilin Province, China., Department of Neurosurgery, The First Hospital of Jilin University, Xinmin Street 71, Changchun, 130021, Jilin Province, China. .

Abstract:  The activity of some constitutive contained in the extracellular signal-regulated kinase (ERK) pathway plays crucial roles in glioma cell growth and proliferation. Emerging studies have reported that microRNA (miRNA) could regulate the ERK signal pathway by directly targeting various oncogenes. This study enabled us to discover that the average miR-126 expression was significantly decreased in glioblastoma tissues, and this significant decrease was related to high histopathological grades. Our experiment also demonstrated that the over-expression of miR-126 suppressed glioma cell proliferation and invasion in vitro. Kirsten rat sarcoma viral oncogene (KRAS) which is involved in ERK pathway was directly targeted by miR-126 in glioma through binding to two sites in the 3' untranslated region (3'-UTR) of KRAS mRNA. Notably, the expression level of KRAS was positively correlated to the activity of ERK pathway and its downstream regulators (phosphorylation level of ERK (pERK) and c-Fos). Furthermore, the over-expression of KRAS expression vector without the 3'-UTR partially reverses the tumor-suppressive effects of miR-126. Moreover, the up-regulation of miR-126 contributes to the aberrant activation of the ERK signaling and inhibits cell proliferation and invasion through targeting KRAS. Therefore, it was suspected that miR-126 may be a potential therapeutic target for high-grade glioma. 

Retracted: No

PubPeer comments:
 Figure 5 appears to have some image duplications ![file] Control and NC resemble each other strongly when NC is flipped horizontally. KRAS and NC resemble each other when KRAS is rotated. There are also some additional duplications which are circled. , 
 Correction to: MiR-126 Regulates the ERK Pathway via Targeting KRAS to Inhibit the Glioma Cell Proliferation and Invasion [10.1007 s12035-021-02302-3] However, this may not solve all the problems in the article. Figure 4. After being flipped horizontally, these bands for u03b2-actin look the same. ![file] , 
 Figure 1 appears to have been cobbled together too. *** ![file] , 
 u201cCorrection to: Mol Neurobiol (2017) 54:137u2013145 https: doi.org 10.1007 s12035-015-9654-8 The original version of this article unfortunately contained some mistakes. This is concerning with our paper titled u201cMiR-126 Regulates the ERK Pathway via Targeting KRAS to Inhibit the Glioma Cell Proliferation and Invasionu201d published in Molecular Neurobiology before. https: doi.org 10.1007 s12035-015-9654-8. Recently, we found there was (sic) technical error (sic) in the images of transwell assay in Fig. 5c in this paper. After full consideration, we decided to make an erratum on the figure based on the rigorous attitude of scholarship. We promise the errata does not change any of the conclusions drawn in the study, and apologize to the editorial board, reviewers and the readership for the inconvenience caused.u201d ![file] , 
 Although I notified the journal almost a year ago about the possibility of further issues in this paper, they do not seem to have published an additional correction. While looking into this paper again, I noticed that the original Control image published in Figure 5 seems to have originally been published in Oncotarget in 2014 (after rotation). There did not appear to be any authors in common. There were a few alterations to the Oncotarget image before its reuse. This Control image was replaced in May 2021 along with the replacement of all of the other images in Figure 5 of this paper. *** ![file] ,