<p>Downregulation of miR-575 Inhibits the Tumorigenesis of Gallbladder Cancer via Targeting p27 Kip1</p>
Authors: Yang Fu
Wunan Mi
Yiyu Qin
Jian Li
Yizheng Zhang
Cheng Huang
Journal: OncoTargets and Therapy (2020)
Discipline: CLINICAL MEDICINE
Keywords: gallbladder cancer; miR-575; p27 Kip1.
Affiliations: Clinical Medical College, Jiangsu Vocational College of Medicine, Yancheng, Jiangsu 224005, People's Republic of China., Department of Gastrointestinal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, People's Republic of China.

Abstract:  Background: Gallbladder cancer (GBC) is the most ﻿common biliary tract malignant cancer ﻿worldwide. It has been reported that microRNA-575 (miR-575) was involved in the tumorigenesis of many cancers. However, the role of miR-575 during the progression of GBC remains largely unknown. Methods: The expression of miR-575 in GBC cells was detected by quantitative real-time polymerase chain reaction. The proliferation of GBC cells was examined by CCK-8 assay and Ki-67 staining. Apoptosis of GBC cells was measured by flow cytometry, and cell invasion was tested by transwell assay. Moreover, protein expressions in GBC cells were evaluated using Western blot. The target gene of miR-575 was predicted using Targetscan and miRDB. Finally, xenograft tumor model was established to verify the function of miR-575 in GBC in vivo. Results: Our findings indicated that miR-575 antagonist decreased the proliferation and invasion of GBC cells. In addition, miR-575 antagonist significantly induced apoptosis of GBC cells via inducing G1 arrest. Meanwhile, p27 Kip1 was found to be a direct target of miR-575 with luciferase reporter assay. Moreover, miR-575 antagonist significantly decreased the expressions of CDK1 and ﻿cyclin E1 and upregulated the levels of cleaved caspase3 and p27 Kip1 in GBC cells. Finally, miR-575 antagonist notably suppressed GBC tumor growth in vivo. Conclusion: Downregulation of miR-575 significantly inhibited the tumorigenesis of GBC via targeting p27 Kip1. Thus, miR-575 might be a potential novel target for the treatment of GBC. 

Retracted: No

PubPeer comments:
 Figure 1 * Green boxes: Several panels in Figure 1D (GBC-SD cells) appear to look identical to panels in Figure 1E (G415 cells). Could the authors check please? ![file] , 
 #1 Thansk very much for pointing out this mistake. We accidentally uploaded the same images twice in Figure 1E due to carelessness and this error do not affect the results of the paper. We have contacted the publisher to report this issue and the corrected Figure 1 was sent as well. We believe the corrigendum will be published very soon. We are very sorry for our carelessness. Yours sincerely, , 
 Corrigendum, 1 December 2020: https: www.dovepress.com corrigendum-downregulation-of-mir-575-inhibits-the-tumorigenesis-of-ga-peer-reviewed-fulltext-article-OTT  *The authors have advised due to an error at the time of figure assembly, Figure 1E on page 3670 is incorrect. The correct Figure 1 is shown below.* *The authors apologize for this error and advise it does not affect the results of the paper.* New figure 1 panels: ![file] I thank the authors for addressing and correcting this duplication. ,