<em>PRMT7</em> contributes to the metastasis phenotype in human non-small-cell lung cancer cells possibly through the interaction with <em>HSPA5</em> and <em>EEF2</em>
Authors: Peng Zhang
Liangcheng Zheng
Deyao Xie
Dezhi Cheng
Zhifeng He
Shangwen Dong
Journal: OncoTargets and Therapy (2018)
Discipline: CLINICAL MEDICINE
Keywords: EEF2; HSPA5; PRMT7; arginine methylation; human non-small-cell lung cancer
Affiliations: Department of Thoracic Surgery, Tianjin Medical University General Hospital, Tianjin, China, ., Department of Thoracic Cardiovascular, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Abstract:  Background: Non-small-cell lung cancer (NSCLC) constitutes the leading cause of cancer death in humans. Previous studies revealed the essential role of the protein arginine methyltransferase 7 (PRMT7) in promoting metastasis in breast cancer. However, its function and potential mechanism in NSCLC remain unclear. Materials and methods: The gene expression of PRMT7 between lung cancer tissues and normal tissues was studied with online database (http://medicalgenome.kribb.re.kr/GENT/). NSCLC cell lines with specific gene overexpression were constructed with lentivirus transduction. Matrigel invasion and colony formation assays were performed to evaluate the invasion and colony formation abilities. Co-immunoprecipitation coupled with mass spectrometry analysis was performed to explore the potential interaction proteins of PRMT7. Bioinformatic analysis was performed with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases. Results: Online analysis of gene expression patterns revealed the relatively high expression of PRMT7 in lung cancer tissues. PRMT7 overexpression was able to promote the invasion and colony formation of A549 and SPC-A1 cells. A total of 19 in-common proteins shared by both NSCLC cell lines were identified to be interacting with PRMT7 and found to participate in a wide variety of pathways and protein-protein interactions according to bioinformatic analysis. Among them, HSPA5 and EEF2 were further investigated for their essential roles in PRMT7-promoted NSCLC cell invasion. Conclusion: Our results suggested PRMT7 overexpression was able to promote metastasis in NSCLC possibly through the interaction with HSPA5 and EEF2, which provides the potential mechanism of oncogenesis in lung cancer. 

Retracted: No

PubPeer comments:
 Figure 2B appears to have two images which overlap after rotation. General areas shown with red. Similar issues in paper with shared authors: https: pubpeer.com publications C0BC2D00ED28924217A8C70CC79DCD *** ![file] , 
 Thank you very much for helping us find out the problem. It is obvious that these two pictures belong to the same sample. I checked the original pictures and found that the shooting time was very tight. Maybe there was an error when our staff took the sample. What has to be criticized is that, our staff s carelessness and negligence led to the wrong pictures. At present, the transfection positive cells are reviving, and the experiment is going on again. , 
 #2 Is the original data no longer available? This is a very recent paper. , 
 #3 #3 Thank you for your time and concern. Actually, this project was done five years ago. I was very worried about the reliability of the data, so I d better arrange to do it again. In addition, the transfection positive cells are frozen well and they are recovering, which can verify the results in 2-3 weeks. , 
 Figure 3 may also have some issues, see below after adjusting contrast on the SPC-A1 image (bottom). *** ![file] , 
 I m not sure about the specific situation, there is a possibility of picture placement error. We looked for the original data, and A549 also got this data. Since there are doubtsuff0cit is better to change this image.![file] ,