Small interfering RNA-induced inhibition of epithelial cell transforming sequence 2 suppresses the proliferation, migration and invasion of osteosarcoma cells
Authors: Yihe Hu
Jie Xie
Pengfei Lei
Journal: Experimental and Therapeutic Medicine (2015)
Discipline: CLINICAL MEDICINE
Keywords: apoptosis; epithelial cell transforming sequence 2; invasion; osteosarcoma; proliferation.
Affiliations: Department of Orthopedics, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.

Abstract:  Osteosarcoma (OS) is the most common malignant tumor in bones. Although the five-year survival rate has improved to ∼60% for patients without metastasis, the prognosis remains poor for patients with metastatic OS. Epithelial cell transforming sequence 2 (ECT2) has been shown to act as an oncogene in human malignancies. More recently, ETC2 was shown to be involved in the development and progression of OS; however, the detailed role of ECT2 in the regulation of cellular biological processes in OS cells remains largely unknown. Therefore, it was investigated in the present study. It was found that the expression of ECT2 was notably increased in OS tissues when compared with that in matched normal adjacent tissues. Furthermore, it was established that the downregulation of ECT2 induced by transfection with ECT2-specific small interfering RNA effectively inhibited OS cell proliferation and induced cell apoptosis. Further investigation revealed that the inhibition of ECT2 expression suppressed OS cell migration and invasion, indicating that the overexpression of ECT2 promotes OS cell migration and invasion, while. In addition, western blotting results indicated that matrix metalloproteinases 2 and 9 may be involved in the ECT2-mediated OS cell invasion. In conclusion, the current study suggested that ECT2 acted as an oncogene in OS, and it may become a promising therapeutic target for the prevention and treatment of OS. 

Retracted: Yes

PubPeer comments:
 Fig 4.  (A) Cell migration and (B) cell invasion assays were performed in MG63 cells with or without transfection with ECT2-specific siRNA . Duplicate panels are marked. ![file] , 
 Fig 3,  Cell apoptosis assay was performed in MG63 cells with or without transfection with ECT2u2011specific siRNA . ![file] For comparison, Fig 2 from [Li et al (2013)] .  AZD8055 increases apoptosis in cervical cancer cells. Following treatment with 10 nM AZD8055 for 24, 48 and 72 h, HeLa cells were stained with FITC-conjugated Annexin V PI. Percentage of Annexin V PI-stained cells was determined by flow cytometry . ![file] , 
 An editor [retraction] was issued on July 9th, 2021: Following the publication of this paper, it was drawn to the Editors attention by a concerned reader that certain of the flow cytometric data shown in Fig. 3 and Transwell cell migration data shown in Fig. 4A and B were strikingly similar to data appearing in different form in other articles by different authors. Owing to the fact that the contentious data in the above article had already been published elsewhere, or were already under consideration for publication, prior to its submission to Experimental and Therapeutic Medicine, the Editor has decided that this paper should be retracted from the Journal. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive any reply. The Editor apologizes to the readership for any inconvenience caused. ,