Down-regulation of microRNA-429 alleviates myocardial injury of rats with coronary heart disease
Authors: Hao Zhang
Jinglin Cheng
Jingrong Li
Qin Yang
Heping Zuo
Qinglong Zhang
Journal: Cell Cycle (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Coronary heart disease; CrkL; MEK/ERK signaling pathway; MicroRNA-429; apoptosis
Affiliations: Emergency Department, Attending doctor, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China, Emergency Department, The second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China, Emergency Department, Chief physician, The second Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China

Abstract:  In recent years, the impact of microRNAs (miRNAs) on coronary heart disease (CHD) has been identified. This study was aimed to investigate the regulative role of microRNA (miR-429) in myocardial injury of rats with CHD. Expression of miR-429 in CHD patients and healthy people was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR). The CHD rat models were injected with normal saline, mimics negative control (NC), miR-429 mimics, inhibitors NC and miR-429 inhibitors twice a week, for 4 weeks. Levels of inflammatory factors, oxidative stress indices as well as apoptosis of cardiomyocytes were determined by a series of assays. Expression of miR-429 was up-regulated in CHD patients. Reduced miR-429 could decline the expression of oxidative stress-related factors and inflammation-related factors, and inhibit the apoptosis of cardiomyocytes in rats with CHD. Moreover, the down-regulation of miR-429 could promote the expression of CrkL and repress activation of the MEK/ERK signaling pathway. This study reveals that restrained miR-429 could exert a protective impact on myocardial injury of rats with CHD by suppressing oxidative stress, inflammation reaction and apoptosis of cardiomyocytes. The function mechanisms may relate to the up-regulation of CrkL and inhibition of the MEK/ERK signaling pathway. 

Retracted: Yes

PubPeer comments:
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 Retracted [17 March 2022] . --------------------------------- We, the Editors and Publisher of the journal *Cell Cycle*, have retracted the following article: Qin Yang, Jingrong Li, Hao Zhang, Heping Zuo, Qinglong Zhang, and Jinglin Cheng. Down-regulation of microRNA-429 alleviates myocardial injury of rats with coronary heart disease. Cell Cycle. 2019;18(19):2550-2565. doi: 10.1080 15384101.2019.1652037. Since publication, concerns have been raised about the integrity of the data in the article. When approached for an explanation, the authors have been unable to address all the concerns raised and have not been able to provide all their original data. As verifying the validity of published work is core to the integrity of the scholarly record, we are therefore retracting the article. The corresponding author listed in this publication has been informed. We have been informed in our decision-making by our policy on publishing ethics and integrity and the COPE guidelines on retractions. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as u2018Retractedu2019. ,