<p>Long Non-Coding RNA LINC01783 Promotes the Progression of Cervical Cancer by Sponging miR-199b-5p to Mediate GBP1 Expression</p>
Authors: Bu-Zhen Tan
Li-Juan Yang
Jie Xue
Liang Xiong
Wei-Jun Chen
Lin Yang
Li Huang
Lin Li
Xiao-Qing Liang
Journal: Cancer Management and Research (2020)
Discipline: CLINICAL MEDICINE
Keywords: LncRNA; ceRNA; cervical cancer; invasion; migration
Affiliations: Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Nanchang University, Reproductive Hospital Affiliated to Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, People's Republic of China., Department of Urinary Surgery, Armed Police Jiangxi General Team Hospital, Nanchang, Jiangxi, People's Republic of China., Department of Reproductive Medicine, Reproductive Hospital Affiliated to Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, People's Republic of China., Department of Gynaecology and Obstetrics, The Fourth Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, People's Republic of China., Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, People's Republic of China.

Abstract:  Background: Long non-coding RNA showed potential regulating effects in oncogenesis. Highly expressed LncRNA LINC01783 is observed in cervical cancer. However, the specific pathogenesis of cervical cancer is still unclear. Methods: Differential lncRNAs in cervical cancer were identified based on TCGA dataset. Subsequently, qRT-PCR was utilized for testing the LINC01783 expression in cervical cancer cell lines and normal human cervical epithelial cell line HcerEpic. CCK-8, EdU, Wound healing assay, Transwell assay and flow cytometry were used for detecting proliferative and migratory potential, cell cycle and apoptosis of cervical cancer cells, respectively. To identify the potential target of LINC01783, bioinformatics assay and dual-luciferase reporter gene assay were performed. Moreover, to clarify their interactions and roles in regulating the progression of cervical cancer, Western blot assay and RIP assay were carried out. Results: Our results revealed LINC01783 is overexpressed in cervical cancer cells. Overexpressed LINC01783 considerably accelerated the cell proliferation, migration and invasion of cervical cancer cells while restrained cell apoptosis of them. Moreover, LINC01783 positively regulated the GBP1 expression via competitively binding to miR-199b-5p. Conclusion: LINC01783 is involved in the progression of cervical cancer through competitively binding to miR-199b-5p to mediate GBP1 expression. 

Retracted: Yes

PubPeer comments:
 Retracted [2 March 2022] . ---------------- At the request of the authors, the Editor and Publisher of *Cancer Management and Research* wish to retract the published article. The authors have informed the journal that they have concerns with the reliability and reproducibility of the published results. In addition, they cannot provide any original data relating to their study and can no longer verify the findings as reported. The Editor has agreed with the request to retract the article. Our decision-making was informed by our policy on publishing ethics and integrity and the COPE guidelines on retraction. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as u201cRetractedu201d. ,