miR-200b inhibits migration and invasion in non-small cell lung cancer cells via targeting FSCN1
Authors: Wenliang Liu
Hui Zhou
Peng Xiao
Journal: Molecular Medicine Reports (2016)
Discipline: CLINICAL MEDICINE
Keywords: NSCLC; microRNA-200b; metastasis; migration; invasion
Affiliations: Department of Thoracic Surgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China., Department of Thoracic Surgery, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China., Department of Medical Oncology, Tumor Hospital of Hunan, Changsha, Hunan 410000, P.R. China.

Abstract:  Distant metastasis of non-small cell lung cancer (NSCLC) leads to high postoperative recurrence and low long‑term survival rates. Deregulation of microRNA (miR)-200b has been demonstrated to be associated with NSCLC metastasis. However, the underlying molecular mechanism of miR‑200b in mediating NSCLC cell migration and invasion remains to be fully elucidated. In the current study, reverse transcription‑quantitative polymerase chain reaction data indicated that miR‑200b was significantly downregulated in several NSCLC cell lines, including A549, L78, H1229, H358 and H1650, compared with a normal human lung epithelial cell line, BEAS‑2B. Overexpression of miR‑200b significantly inhibited NSCLC cell migration and invasion. Bioinformatics analysis and a luciferase reporter assay were additionally conducted, which identified fascin actin‑bundling protein 1 (FSCN1) as a novel target of miR‑200b. In addition, miR‑200b negatively mediated the protein expression of FSCN1 in NSCLC H1229 cells. siRNA‑mediated FSCN1 inhibition also significantly inhibited the migration and invasion of H1229 cells. In addition, overexpression of FSCN1 effectively reversed the suppressive effect of miR‑200b overexpression on NSCLC cell migration and invasion. Accordingly, it is suggested that miR‑200b is able to inhibit the migration and invasion of NSCLC cells, partly at least, via targeting FSCN1. The current study provides novel insight into miR‑200 regulation in NSCLC metastasis. 

Retracted: Yes

PubPeer comments:
 Figs 2C and 5.  Cell ... invasion were then examined by conducting a ... Transwell assay in each group . ![file] For comparison: *[upper]* Figs 4 C D and 5C from [Yang et al (2016)] .  Transwell assays were conducted to examine the cell invasion capacities ofA549 cells transfected with (C) miR-133b mimics and (D) FSCN1 plasmids. Non-transfected A549 cells were used as control ....  Transwell assay were conducted to examine the cell migration and invasion in each group . *[lower]* Fig 5B from [Wang et al (2016)] .  Transwell assay to determine the invasive capacity of U2OS cells transfected with miRu2011101 mimics or cu2011FOS siRNA, or cou2011transfected with miRu2011101 mimics and cu2011FOS plasmid. Magnification, x100. Control: untransfected U2OS cells . ![file] Fig 7 from [Wang et al (2014)] .  Cell invasion assay was performed to determine the effect of miR-195 and CARMA3 on invasion in SW480 and HT29 cells following transfection with an miR-195 mimic or an miR-195 inhibitor, or co-transfected with an miR-195 mimic and CARMA3-pcDNA3.1 . ![file] , 
 Retracted [14 June 2021] . More background [here] . --------------------------------------- Following the publication of this paper, it was drawn to the Editorsu2019 attention by a concerned reader that certain of the Transwell assay data shown in Figs. 2C and 5C bore unexpected similarities to data appearing in different form in other articles by different authors. Owing to the fact that some of the contentious data in the above article had already been published elsewhere, or were already under consideration for publication, prior to its submission to Molecular Medicine Reports, the Editor has decided that this paper should be retracted from the Journal. After having been in contact with the authors, they agreed with the decision to retract the paper. The Editor apologizes to the readership for any inconvenience caused. ,