Resveratrol inhibits the invasion and metastasis of colon cancer through reversal of epithelial‑ mesenchymal transition via the AKT/GSK‑3β/Snail signaling pathway
Authors: Kai Zhang
Harpreet S Wasan
Leitao Sun
Hong Huang
Minhe Shen
Dawei Huang
Shanming Ruan
Li Yuan
Shenglin Ma
Mengmeng Zhou
Journal: Molecular Medicine Reports (2019)
Discipline: CLINICAL MEDICINE
Keywords: drugs; tumor invasion; metastasis; colon cancer; resveratrol
Affiliations: The First Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China., Department of Traditional Chinese Medicine, The First People's Hospital of Quzhou, Quzhou, Zhejiang 324000, P.R. China., Department of Chinese Medicine, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, P.R. China., Department of Cancer Medicine, Hammersmith Hospital, Imperial College Healthcare NHS Trust, London W12 0HS, UK., Teaching and Research Section of Prescription, Basic Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China., Department of Medical Oncology, The Fourth Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, P.R. China., Department of Medical Oncology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, P.R. China.

Abstract:  The identification of safe and effective drugs that inhibit tumor invasion and metastasis is required to improve the clinical outcome of patients with colon cancer. The present study aimed to investigate the inhibitory effects and possible mechanisms of action of resveratrol against the invasion and metastasis of colon cancer. AKT1‑knockdown SW480 and SW620 colon cancer cells were used to detect the effects of resveratrol on cell invasion and metastasis, as well as changes in the expression of epithelial‑mesenchymal transition (EMT) markers and serine/threonine kinase (AKT)/glycogen synthase kinase (GSK)‑3β/Snail signaling pathway‑related molecules in vitro. Furthermore, nude mice were inoculated with SW480 cells in the tail vein to establish an in vivo lung metastasis model of colon cancer, to investigate the effects of resveratrol on lung metastasis in colon cancer. The results revealed that resveratrol treatment and AKT1 knockdown significantly inhibited cell migration and invasion in colon cancer, and markedly increased E‑cadherin expression and decreased that of N‑cadherin, phospho (p)‑AKT1, p‑GSK‑3β, and Snail in colon cancer both in vitro and in vivo. Furthermore, the effects of resveratrol were significantly weaker in the AKT1‑knockdown cells. In conclusion, resveratrol may suppress the invasion and metastasis of colon cancer through reversal of EMT via the AKT/GSK‑3β/Snail signaling pathway. AKT1 may therefore be a key regulator of EMT in colon cancer cells and a potential therapeutic target for this disease. 

Retracted: No

PubPeer comments:
 Unexpected similarities in figure 5A. *** ![file] , 
 Also seems to be an issue in Figure 4A. *** ![file] , 
 Corrigendum, [10 October 2022] . ------------------------------ Subsequently to the publication of the above article, an interested reader drew to the authorsu2019 attention that the Ncu2011control and siu2011AKT1u2011control data panels featured in Fig. 4A for the SW480 Transwell assay experiments on p. 2788 appeared to contain overlapping data, such that the data, which were intended to show the results from experiments performed under different experimental conditions, may have been derived from the same original source. Furthermore, in Fig. 5 on p. 2789, there appeared to be some overlapping data comparing the AKT1 western blotting data with the pu2011AKT1 data, and the same data also appeared in Fig. 4D for the pu2011AKT1 data presented there. The authors have reu2011examined their original data, and have realized that these figures were assembled incorrectly; essentially, the siu2011AKT1u2011control data panel was selected incorrectly for Fig. 4A, and the authors were able to retrieve the original data for the western blots presented in Fig. 5. The corrected versions of Figs. 4 and 5 are shown on the next page. The authors confirm that these errors did not have any major impact on the conclusions reported in their paper, and are grateful to the Editor of Molecular Medicine Reports for allowing them this opportunity to publish a Corrigendum. Furthermore, the authors apologize to the readership for any inconvenience caused. , 
 The authors may also wish to examine similarities between data published in this paper and another paper with common authors submitted around the same time. For example, a gel strip in Figure 6D of Journal of Cancer (2019), doi: 10.7150 jca.32873, seems to appear in Figure 5D of this MMR paper. *** ![file] , 
 Another apparent overlap between these two papers. *** ![file] ,