MiR-221 affects proliferation and apoptosis of gastric cancer cells through targeting SOCS3
Authors: Q-Y Zhou
P-L Peng
Y-H Xu
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: SOCS3; miR-221; gastric cancer; proliferation; apoptosis
Affiliations: Department of Pathology, The Central Hospital of Enshi Autonomous Prefecture, Enshi, China. .

Abstract:  Objective: The suppressors of cytokine signaling 3 (SOCS3) negatively regulates the JAK-STAT pathway. The bioinformatics analysis revealed a targeted binding site between miR-221 and the 3'-UTR of SOCS3 mRNA. This study investigated the role of miR-221 in the proliferation and apoptosis of gastric cancer cells. Patients and methods: The Dual-Luciferase reporter gene assay validated the target relationship between miR-221 and SOCS3. Gastric cancer tissues were collected and compared with adjacent tissues to detect the expression of miR-221 and SOCS3. The Kaplan-Meier method was used to analyze the survival rate between patients with high and low miR-221 expression. Human gastric cancer SGC7901 cells were cultured and divided into the miR-NC group and miR-221 inhibitor group, followed by an analysis of the expression of miR-221, SOCS3, p-JAK2 and p-STAT3, cell apoptosis, and proliferation. Results: Compared with adjacent tissues, miR-221 expression was significantly increased in tumor tissues, and SCOS3 mRNA expression was decreased. Compared with those with lower miR-221 expression, the prognosis of patients with higher miR-221 expression was significantly worse. There was a targeted regulatory relationship between miR-221 and SOCS3 mRNA. Compared with GES-1 cells, miR-221 expression in gastric cancer MGC803 and SGC7901 was significantly increased, and the expression of SOCS3 mRNA and protein was significantly decreased. The transfection of miR-221 inhibitor significantly increased SOCS3 expression in gastric cancer SGC7901 cells, decreased p-JAK2, p-STAT3 protein expression, increased cell apoptosis, and decreased cell proliferation. Conclusions: Increased miR-221 expression and decreased SOCS3 expression are related to gastric cancer. MiR-221 regulates the proliferation and apoptosis of gastric cancer cells by regulating SOCS3 expression. 

Retracted: Yes

PubPeer comments:
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 Some time in the last week or so, the journal wished this paper into the cornfield: https: www.europeanreview.org article 22226 Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cMiR-221 affects proliferation and apoptosis of gastric cancer cells through targeting SOCS3, by Q.-Y. Zhou, P.-L. Peng, Y.-H. Xu, published in Eur Rev Med Pharmacol Sci 2019; 23 (21): 9427-9435u2013DOI: 10.26355 eurrev_201911_19436u2013PMID: 31773681u201d has been withdrawn. The Publisher apologizes for any inconvenience this may cause. ![file] ![file] Really? , 
 The National Medical Commission of the PRC announced the results of an investigation, [15 October 2021](http: www.nhc.gov.cn cms-search xxgk getManuscriptXxgk.htm?id=506681bfbc444f7ba1f053b5d4111f50). Thanks to Google Translate for the translation. ---------------------------- 1. The paper published by Xu Yanhua, the corresponding author of the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Hubei Province, and Zhou Qiuyuan as the first author. MiR-221 affects proliferation and apoptosis of gastric cancer cells through targeting SOCS3 After investigation, Zhou Qiuyuan, the first author of the paper, had academic misconduct of buying and selling data and fabricating the research process, and corresponding author Xu Yanhua had academic misconduct of improper signature. Relevant persons in charge shall be dealt with as follows: - 1. To the first author Zhou Qiuyuan: talk about scientific research integrity and admonishment; report criticism at the hospital weekly meeting; not be promoted within five years, withdraw from the Enshi Central Hospital s Young Talent Growth Project, and return the income from this article Hospital awards; withdrawn; disqualification of this year s first model evaluation. - 2. Corresponding author Xu Yanhua: Talks about scientific research integrity, circulates criticisms, and cannot be promoted within one year, and the qualifications of this year s model evaluation will be cancelled. (Notification link: https: www.es9e.com show-49-9232-1.html ) ,