MiR-488 inhibits proliferation and cisplatin sensibility in non-small-cell lung cancer (NSCLC) cells by activating the eIF3a-mediated NER signaling pathway
Authors: Ji-Ye Yin
Hsuan-Shun Huang
Hong-Hao Zhou
Wei Zhang
Chao Fang
Na-Yiyuan Wu
Zhao-Qian Liu
Xiang-Ping Li
Yi-Xin Chen
Journal: Scientific Reports (2017)
Discipline: Multidisciplinary
Keywords: eIF3a; miRNA-488; cisplatin resistance; lung cancer; NSCLC
Affiliations: Departments of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, P. R. China., Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, P. R. China., Departments of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, P. R. China., Department of Research, Cervical Cancer Prevention Center, Tzu Chi University, Hualien 970, Taiwan, Republic of China., Hunan Province Cooperation Innovation Center for Molecular Target New Drug Study, Hengyang 421001, P. R. China.

Abstract:  Our previous studied indicated that eukaryotic translation initiation factor 3a (eIF3a) increases the sensitive of platinum-based chemotherapy in lung cancer. MiRNAs play an important role in lung carcinogenesis and drug response. In this study, we aimed to identify potential endogenous miRNAs that inhibit eIF3a expression and determine their influence of this inhibition on cisplatin resistance. Using bioinformatics analysis prediction and confirmation with dual-luciferase reporter assays, we found that miRNA-488 inhibited eIF3a expression by directly binding to the 3'UTR of eIF3a. In addition, the overexpression of miRNA-488 inhibited cell migration and invasion in A549 cells, and also inhibited cell proliferation, cell cycle progression by elevated P27 expression. Compared to the parental cell line, A549/cisplatin (DDP) resistant cells exhibited a higher level of miRNA-488. Moreover, we found that miRNA-488 was associated with cisplatin resistance in three NSCLC cells (A549, H1299 and SK-MES-1). The mechanism of miRNA-488 induced cisplatin resistance was that miRNA-488 activated nucleotide excision repair (NER) by increasing the expression of Replication Protein A (RPA) 14 and Xeroderma pigmentosum group C (XPC). In conclusion, our results demonstrated that miRNA-488 is a tumor suppressor miRNA that acts by targeting eIF3a. Moreover, miRNA-488 also participates in eIF3a mediated cisplatin resistance in NSCLC cells. 

Retracted: No

PubPeer comments:
 Figure 4D appears to have a repeated image, after rotation. *** ![file] , 
 Dear Prof. Actinopolyspora Biskrensis, Thank you very much for your helpful comments. We checked the article carefully again and sorry for the mistake caused by our negligence. Fortunately, it does not affect the main results and conclusions of the article. We have contacted the editorial department to publish a corrigendum with update the correct figure 4d for our paper. Thanks again for your comment! Kind regards! , 
 Dear Prof. Actinopolyspora Biskrensis, Thank you very much for your helpful comments. We checked the article carefully again and sorry for the mistake caused by our negligence. Fortunately, it does not affect the main results and conclusions of the article. We have contacted the editorial department to publish a corrigendum with update the correct figure 4d for our paper. Thanks again for your comment! Kind regards! , 
 Author Correction Published: 11 November 2021 This Article contains an error in Figure 4D, where the image for the blank condition was inadvertently duplicated for the NC panel. The correct Figure 4 and its accompanying legend appear below. ![file] ,