Long noncoding RNA LINC00205 enhances the malignant characteristics of retinoblastoma by acting as a molecular sponge of microRNA-665 and consequently increasing HMGB1 expression
Authors: Yin Hu
Jian Long
Shu Zhang
Journal: Biochemical and Biophysical Research Communications (2020)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: Long intergenic non–protein-coding RNA 00205; MicroRNA-665; Retinoblastoma.
Affiliations: Department of Ophthalmology, The First People's Hospital of Jinzhou (Yangtze University Affiliated First People's Hospital), Hubei, 434000, PR China., Department of Oncology, Jingzhou Central Hospital, The Second Clinical Medical College of Yangtze University, Hubei, 434020, PR China. Electronic address: ., Department of Ophthalmology, Xiangyang Central Hospital, Hubei, 441021, PR China. Electronic address: .

Abstract:  Long intergenic non-protein-coding RNA 00205 (LINC00205) has been found to play crucial roles in hepatocellular carcinoma progression. In this study, we aimed to determine the expression pattern of LINC00205 in retinoblastoma (RB), to identify its functions in RB progression in detail, and to reveal the underlying mechanisms. Herein, we showed that LINC00205 is highly expressed in RB tissues and cell lines. The LINC00205 upregulation correlated with adverse clinicopathological parameters and shorter overall survival in patients with RB. LINC00205 depletion decreased the proliferative, migratory, and invasive abilities; promoted the apoptosis of RB cells in vitro; and impeded the tumor growth of RB cells in vivo. Mechanism investigation revealed that LINC00205 can act as a competing endogenous RNA by sponging microRNA-665 (miR-665) in RB cells, thereby upregulating miR-665's target: high-mobility group box 1 (HMGB1). Finally, rescue experiments confirmed that enhancing the miR-665-HMGB1 axis output attenuated the influence of the LINC00205 knockdown on RB cells. To sum up, the newly identified LINC00205-miR-665-HMGB1 pathway was systematically studied and may be validated as a potential target for RB diagnosis, prognosis, and therapy. 

Retracted: No

PubPeer comments:
 Figs 1F, 4B, 4G. ![file] A panel is duplicated, with what appear to be different descriptions (blue boxes). Yellow box marks a panel that also appears in Figs 2C,5C,6C of [Cheng et al (2020)] , and in Figs 2D,6D of [Lv et al (2019)] . ![file] ![] ![] There may be matches with other panels, or with other papers in this oeuvre. https: docs.google.com spreadsheets d 1X401JM6CcpTSkbKsPsOl20pRKQ-ns1S_J5pTwtBjqPw edit#gid=0 , 
 Figs 1F, 4B, 4G again. ![] Carmine box added, to match a panel from Figs 2C, 4D, 5E from [Zhao, Li Uu (2019)] . ![] ![] , 
 Another in a series of  Travelling Ruler posts. These six line-ups of xenograft tumors came from different research teams at different institutions, but somehow they seem to use the same ruler to provide scale. **Fig 1J from [Zhang, Long Hu (2020)] **. Representative images of the tumor xenografts obtained from groups u201csh-LINC00205u201d and u201csh-NCu201d. ![file] Fig 7B from [Liu et al (2020)] .  Images of tumor xenografts derived from sh-OSER1-AS1 or sh-NC-stably transected H522 cells . ![file] Fig 7B from [Yang et al (2020)] .  After 4 weeks, all the mice were euthanized, and the tumor xenografts were excised and photographed. ![file] Fig 6B of [Xing et al (2020)] .  The tumor xenografts obtained from sh-LINC01224 or sh-NC groups. ![file] Fig 6B from [Qi, Lu Wang (2020)] .  Representative photograph of tumor xenografts obtained from the sh-ST7-AS1 and sh-NC groups . ![file] Fig 7C from [Hou, Dong Du (2020)] .  Tumor xenografts representative images collected from groups u2018shLINC00466u02b9 and u2018sh-NCu2019. ![file] Of course there are a lot of metal rulers of any given brand within laboratories, but the pattern of smudges and mottling on these six is similar enough to suggest identity. ![file] , 
 #3 *[left]* Fig 1J again. *[right]* Fig 7B from [Zhang et al (2020)] .  Tumor xenografts derived from sh-NR2F1-AS1 or sh-NC stably transfected H460 cells were collected and photographed. ![file] Could the authors of either paper comment on the appearance of the same line-up of xenograft tumors? , 
 A triad of flow-cytometry plots share most of their points in common, and are presumably derived from the same raw data, differently gated and filtered. ![] Right-hand panel is from Fig 1F. Left-hand panel is from Figs 2C, 5B of [Wang et al (2020)] . Middle panel is from Fig 5C of [Xie et al (2019)] . In context: ![] --------------------------------------------------------------- ![] --------------------------------------------------- ![] , 
 - *[above]* Fig 1G. - *[below]* Fig 2I from  Long Non-Coding RNA DARS-AS1 Contributes to Prostate Cancer Progression Through Regulating the MicroRNA-628-5p MTDH Axis ([Fan et al 2020] ) **[retracted]**. ![file] ---------------- - *[left]* Fig 1H. - *[right]* Fig 7G from  LINC00514 drives osteosarcoma progression through sponging microRNA-708 and consequently increases URGCP expression ([Yu et al 2020] ). ![file] , 
 - *[left]* Fig 4C. - *[right]* Fig 3D from  Long Non-Coding RNA C1QTNF1 Antisense RNA 1 Upregulates Hexokinase 2 by Sponging microRNA-484 to Promote the Malignancy of Colorectal Cancer ([Jin et al 2020] ) **[retracted]**. ![file] ,