MicroRNA-505-5p functions as a tumor suppressor by targeting cyclin-dependent kinase 5 in cervical cancer
Authors: Wenhua Tan
Indira Sakhautdinova
Elena Kapora
Bo Gao
Wei Liu
Shujun Feng
Journal: Bioscience Reports (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: CDK5; Cervical cancer; cell invasion; cell migration; cell proliferation
Affiliations: Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China., First department of Gynecology and Obstetrics, Bashkir State Medical University, Ufa, Republic of Bashkortostan 450008, Russia., Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, China .

Abstract:  MicroRNAs (miRs) are considered to be tumor suppressors or oncogenes as they regulate cell proliferation, migration, invasion, and differentiation. Recently, microRNA-505 (miR-505) has been reported as being involved in the progression of several human cancers. In the present study, we aim to investigate the expression rate and functional role of miR-505-5p in cervical cancer (CC) to determine its significance regarding the disease's development.The expression of miR-505-5p and cyclin-dependent kinase 5 (CDK5) in specimens of patients with CC and CC cell lines was examined by quantitative real-time PCR (qRT-PCR) and Western Blot. The relationship between miR-505-5p and CDK5 was verified by luciferase reporter assay. Cell counting kit-8 (CCK-8) assay, Scratch wound healing assay and transwell assay were used to detect the roles of miR-505-5p and CDK5 in CC cell functions. Western Blot was utilized to explore the epithelial-mesenchymal transition (EMT) markers.The result showed that in CC tissues and CC cell lines miR-505-5p was down-regulated while CDK5 level was up-regulated. MiR-505-5p was closely correlated with the metastasis-associated clinicopathological features. Overexpression of miR-505-5p inhibited cell viability, cell metastasis and EMT in CC cells. CDK5 was confirmed as a direct target of miR-505-5p and inverse relationship between them was also observed. Overexpression of CDK5 reduces the inhibitory effects of miR-505-5p in CC.Taken together, these results determine that miR-505-5p is a tumor suppressor miRNA which regulates tumor cell proliferation, migration, and invasion via binding to the functional target CDK5 and demonstrates its potential for future use in the treatment of CC. 

Retracted: No

PubPeer comments:
 Figure 5F appears to have two images which overlap. This is another paper where the corresponding authoru2019s email address does not match the authoru2019s name (here it is all numerals). *** ![file] *** ![file] , 
 The Editorial Office has been made aware of potential issues surrounding the scientific validity of this paper, hence has issued an expression of concern to notify readers whilst the Editorial Office investigates. , 
 Retracted [14 April 2021] . ---------------------------------- This Retraction follows an Expression of Concern relating to this article previously published by Portland Press. This article is being retracted from Bioscience Reports at the request of the Editor in Chief and the Editorial Board following receipt of a notification from a reader, alerting the Editorial Board to duplication in Figure 5F. The Editorial Office has also identified evidence of potential image manipulation in this Figure. The authors were contacted regarding the concerns raised and provided a replacement Figure 5F, however given that the original image showed signs of potential manipulation the Editorial Board stands by the decision to retract. The authors do not agree that the images were deliberately manipulated, but agree to the retraction. ,