Inhibiting lncRNA ROR suppresses growth, migration and angiogenesis in microvascular endothelial cells by up-regulating miR-26
Authors: Z-L Xin
W-W Qin
H-Q Wang
X Wang
X-Y Li
K-P Wang
Journal: European review for medical and pharmacological sciences (2018)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: Lower extremity arteriosclerosis; microvascular endothelial cells; long non-coding RNA ROR; growth; migration
Affiliations: Department of Vascular Surgery, Jining No.1 People's Hospital, Jining, Shandong Province, China. .

Abstract:  Objective: Lower extremity arteriosclerosis is one of leading causes of death worldwide. Arteriosclerosis is closely related to microvascular endothelial cells. This study was aimed to explore the role of long non-coding RNA ROR in regulations of growth, migration, and angiogenesis of microvascular endothelial cells. Materials and methods: Angiogenesis was determined by the number of tube-like cells on a matrigel extracellular matrix. Cell viability, apoptosis, and migration were determined by CCK-8 assay, PI/FITC-Annexin V staining method, and transwell assay, respectively. Relative RNA expression of ROR, miR-26, and angiogenesis-associated genes were analyzed by qRT-PCR. The protein expression of apoptosis- and angiogenesis-associated genes, as well as main factors in NF-κB and JAK1/STAT3 pathways, were analyzed by Western blot. Results: LncRNA ROR silence inhibited viability, migration, and angiogenesis of HMEC-1 cells but promoted apoptosis of them. miR-26 expression was promoted after knocking down ROR expression. miR-26 overexpression enhanced the inhibitory effects of ROR silence on growth, migration, and angiogenesis in HMEC-1 cells, whereas, miR-26 silence impaired the effects of ROR silence. Finally, we found that NF-κB and JAK1/STAT3 signaling pathways were inhibited by ROR down-regulation. Similarly, miR-26 overexpression enhanced the inhibitory effect of ROR down-regulation on the pathways and miR-26 inhibition abrogated it. Conclusions: Down-regulating lncRNA ROR inhibited growth, migration and angiogenesis of microvascular endothelial cells possibly through up-regulation of miR-26. During this process, the activations of NF-κB and JAK1/STAT3 pathways were inhibited after interaction of ROR and miR-26. 

Retracted: No

PubPeer comments:
 There seems to be an image that appears in both Figure 3B and Figure 11E, but it is described differently. *** ![file] , 
 An image in Figure 3A and Figure 6E appear to overlap. TAM. *** ![file] , 
 Thank you for your kind reminder. Especially, great thanks for this comment. We have checked all the original pictures again and make sure that all the pictures and data we used in our manuscript are true and correct. However, we did make a mistake when we did Figure 6 and 11 although it didnu2019t have any impact on our results. In the process of preparing the Figure 6 and 11, we misused the data in Figures 3. Therefore, Figure 6 contains the representative images of cell migration in the Figures 3 and Figure 11 contains the representative images of angiogenesis of Figure 3. However, the conclusions made are not influenced because the statistical analysis was performed based on the original images. We have started contacting the periodical office for corrigendum. Additionally, We apologize for this mistake. ,