Construction of a disulfide-stabilized diabody against fibroblast growth factor-2 and the inhibition activity in targeting breast cancer
Authors: Jinxia Zhang
Likuan Xiong
Jiangchuan Zhong
Yaxiong Cai
Haowu Jiang
Ning Deng
Yanrui Deng
Yunfei Yu
Xuejun Lao
Yiye Liang
Journal: Cancer Science (2016)
Discipline: CLINICAL MEDICINE
Keywords: Breast cancer; FGF-2; Pichia pastoris; ds-Diabody; tumor angiogenesis.
Affiliations: Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Biomedicine Translational Institute in Jinan University, Guangzhou, China., Department of Gastrointestinal Surgery, the First Clinical School in Jinan University, Guangzhou, China., Shenzhen Key Laboratory of Birth Defects in Baoan Maternal and Child Health Care Affiliated Hospital in Jinan University, Shenzhen, China.

Abstract:  Fibroblast growth factor-2 (FGF-2) is one of the most important angiogenic factors to promote tumor growth, progression and metastasis. Neutralizing antibodies against FGF-2 may suppress the growth of tumor cells by blocking the FGF-2 signaling pathway. In this study, a disulfide-stabilized diabody (ds-Diabody) that specifically targets FGF-2 was designed. Compared to its parent antibody, the introduction of disulphide bonds in the diabody could significantly increase the stability of ds-Diabody and maintain its antigen binding activity. The ds-Diabody against FGF-2 could effectively inhibit the tube formation and migration of vascular endothelial cells and block the proliferation and invasion of human breast cancer cells. In the mouse model of breast cancer xenograft tumors, the ds-Diabody against FGF-2 could significantly inhibit the growth of tumor cells. Moreover, the densities of microvessels stained with CD31 and lymphatic vessels stained with LYVE1 in tumors showed a significant decrease following treatment with the ds-Diabody against FGF-2. Our data indicated that the ds-Diabody against FGF-2 could inhibit tumor angiogenesis, lymphangiogenesis and tumor growth. 

Retracted: No

PubPeer comments:
 Figure 11A. * Red boxes: Two panels look more similar to each other than expected. ![file] , 
 Dear Elisabeth M Bik, We are sincerely sorry for the problem you have mentioned in the Figure 11A of the article u201cConstruction of a Disulfide-stabilized diabody against FGF-2 and the Inhibition Activity in Targeting Breast Canceru201d. When we submitted the paper, we misused two pictures in Figure 11A and combined them into one big picture due to our negligence. We found the original picture, hoping to replace the two wrong pictures in the red frame. The original picture could be found in the attachment. We sincerely apologize for the confusion and trouble we have caused to you. ![file] , 
 Dear Dr. Deng, Thank you for finding the correct photo. It is great you post it here, but could I also please ask you to start the process of correcting the paper, so that other readers will also benefit from this correction? , 
 Dear lisabeth M Bik, We will contact the journal editor department to correct it, Thank you! , 
 As promised by the authors, there is now a Correction, August 2021, found here: https: onlinelibrary.wiley.com doi 10.1111 cas.15077  *In an article1 titled u201cConstruction of a disulfide-stabilized diabody against fibroblast growth factor-2 and the inhibition activity in targeting breast canceru201d by Yaxiong Cai, Jinxia Zhang, Xuejun Lao, Haowu Jiang, Yunfei Yu, Yanrui Deng, Jiangchuan Zhong, Yiye Liang, Likuan Xiong and Ning Deng, the authors would like to correct the 2nd and 3rd images (top) in Figure 11A as both images are identical in the published version. The correct Figure 11A is presented below.* I thank the authors and journal for addressing this error. ,