MicroRNA-143 suppresses the proliferation and metastasis of human gastric cancer cells via modulation of STAT3 expression
Authors: Yuanyu Wu
Caixia Sun
Youmao Tao
Zheyu Song
Xin Zhao
Xiaoyu Wan
Zhonghang Xu
Journal: American journal of translational research (2020)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: Gastric cancer; apoptosis; miRNA-143; migration; tumor suppressor.
Affiliations: Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital, Jilin University Changchun 130033, Jilin, China., Department of Tumor Surgery, Jilin University Second Hospital Changchun 130000, Jilin, China., Department of Pediatric Respiratory, The First Hospital of Jilin University Changchun 130021, Jilin, China.

Abstract:  Accumulating evidences suggest that miRNAs may prove essential therapeutic targets for the treatment of cancer. Herein, the role and therapeutic implications of miRNA-143 was investigated in gastric cancer. The results revealed miRNA-143 to be aberrantly downregulated in gastric cancer cell lines. Ectopic expression of miRNA-143 resulted in a significant (P&lt;0.05) inhibition of AGS gastric cancer cell proliferation suggestive of the tumor suppressive role of miRNA-143. The inhibition of AGS cell proliferation was mainly via activation of apoptotic cell death as evident from the AO/EB and annexin V/PI staining. Additionally, miR-143 overexpression also caused a significant (P&lt;0.05) decline in the migration and invasion of AGS cells. TargetScan analysis and the dual luciferase assay revealed STAT3 to be the potential target of miRNA-143 in AGS cells. Analysis of STAT3 expression in gastric cancer cell lines showed upto 3.5 fold upregulation of STAT3. However, miRNA-143 overexpression resulted in considerable downregulation of STAT3 expression. Silencing of STAT3 also resulted in the inhibition of cell proliferation, migration and invasion of the AGS cells. Moreover, overexpression of STAT3 could nullify the tumor suppressive effects of miRNA-143 in AGS cells. Taken together, miRNA-143 has a tumor suppressive role in gastric cancer and may prove essential in gastric cancer treatment. 

Retracted: Yes

PubPeer comments:
 The 2015 Springer chapter:  **Alternatives to Cyber Warfare: Deterrence and Assurance**  LtGen Robert J. Elder, USAF (ret), USAF chief scientist Alexander H. Levis, and Bahram Yousefi [George Mason University]  Cyber Warfare. Advances in Information Security, vol 56 copied, without quotation marks and without citation, approximately half of the 2014 ECMS copyrighted paper:  **Multi-Formalism Modeling For Evaluating The Effect Of Cyber Exploits**  28th European Conference on Modelling and Simulation, Brescia, Italy Robert J. Elder, author of the 2015 version, was not an author of the 2014 version. The 2014 version had copied verbatim from the 1988 paper by Jean-Louis M. Grevet et al., which is discussed elsewhere on PubPeer:  https: pubpeer.com publications C440CE492E182ED2A82C6941AE21A5  The doubly copied passages from the 1988 Grevet paper are shown below in green. The work was funded by the US Army Research Office through Contract No. W911NF-13-1-0154 to Carnegie Mellon University and sub-award No.1130163-311619 to George Mason University. The 2015 version of the work claimed novelty, using the same language found in the 2014 version: A new approach based on multi-formalism modeling to model, analyze, and evaluate the effect of cyber exploits on the coordination in organizations has been presented. ... Two new measures of performance, information consistency and synchronization, were used ... The 2015 Springer editor [Sushil Jajodia] directs the Center for Secure Information Systems, George Mason University. Examples are shown below, with the 2014 ECMS copyrighted version on the left. ![file] ![file] ![file] ![file] ![file] ![file] ![file] ![file] ![file] ![file] ![file] ![file] ![file] ![file] ![file] ,