The Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas
Authors: Jitka Cejkova
Barbora Hermankova
Céline Olmiere
Jan Kossl
Sarka Kubinova
Vladimir Holan
Cestmir Cejka
Journal: Oxidative Medicine and Cellular Longevity (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: trehalose; antioxidant; UVB-irradiated corneas; oxidative stress; inflammation
Affiliations: Institute of Experimental Medicine of the Czech Academy of Sciences, Vídenska 1083, 14220 Prague 4, Czech Republic., Faculty of Natural Science, Charles University, Vinicna 7, 12843 Prague 2, Czech Republic., Laboratoires Thea (Thea-Pharma), 12 Rue Louis-Bleriot, 63000 Clermont-Ferrand, France.

Abstract:  Our previous research revealed that trehalose, a nonreducing disaccharide of glucose and an important stress responsive factor, proved to have anti-inflammatory, antiapoptotic, and particularly antioxidant properties in UVB-irradiated corneas. Trehalose reduced oxidative stress in corneas induced by UVB irradiation, by means of a decrease in the antioxidant/prooxidant imbalance in the corneal epithelium. In this study, we demonstrate that trehalose of 3% or 6% concentration in eye drops directly decreases oxidative stress in UVB-irradiated corneas, by removing the excessive amount of reactive oxygen species (ROS). Trehalose drops applied on corneas during UVB irradiation once daily for four days resulted in a reduction or even absence of the oxidative stress, DNA damage, and peroxynitrite formation (detected by nitrotyrosine residues), seen in buffer-treated corneas. Furthermore, trehalose treatment applied curatively after repeated irradiation for the subsequent fourteen days led to the renewal of corneal transparency and significant suppression or even absence of neovascularization. This was in contrast to buffer-treated irradiated corneas, where the intracorneal inflammation was developed and the untransparent corneas were vascularized. In conclusion, the treatment of UVB-irradiated corneas with trehalose eye drops removed the excessive amount of ROS in the corneal epithelium, leading to the suppression of oxidative stress and favorable corneal healing. The 6% trehalose showed a higher intensive antioxidant effect. 

Retracted: Yes

PubPeer comments:
 2020 retraction. https: www.hindawi.com journals omcl 2020 4128985 Oxidative Medicine and Cellular Longevity has retracted the article titled u201cThe Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneasu201d [1] due to concerns with the figures. We were contacted by an author of the article who identified that Figure 4c in [1] is duplicated with 6a in [2], despite different experimental conditions being described. Figure 6c in [1] is also duplicated with Figure 5d in [2] despite different experimental conditions being described. The first author responded with an explanation and revised figures, but these did not satisfactorily address the concerns of the editorial board. The article is therefore being retracted due to concerns regarding the reliability of the data. The authors Sarka Kubinova and Cu00e9line Olmiere agree to the retraction of the article, however, all other authors do not agree. References C. Cejka, J. Kossl, B. Hermankova et al., u201cThe Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas,u201d Oxidative Medicine and Cellular Longevity, vol. 2019, Article ID 1857086, 10 pages, 2019. V. Holan, P. Trosan, C. Cejka et al., u201cA Comparative Study of the Therapeutic Potential of Mesenchymal Stem Cells and Limbal Epithelial Stem Cells for Ocular Surface Reconstruction,u201d Stem Cells Translational Medicine, vol. 4, no. 9, pp. 1052u20131063, 2015. ,