Protective effects of pretreatment with oleanolic acid in rats in the acute phase of hepatic ischemia-reperfusion injury: role of the PI3K/Akt pathway
Authors: Zeping Xu
Yanning Qian
Hongbin Sun
Bo Gui
Fuzhou Hua
Jie Chen
Journal: Mediators of Inflammation (2014)
Discipline: IMMUNOLOGY
Keywords: Oleanolic acid; liver disorders; hepatic ischemia-reperfusion; Sprague-Dawley rats; sodium carboxymethylcellulose
Affiliations: Department of Anesthesiology, 1st Affiliated Hospital, Nanjing Medical University, Nanjing, China ; Key laboratory of Anesthesiology, Xuzhou, Jiangsu, China., Department of Anesthesiology, 2rd Affiliated Hospital, Nanchang University, Nanchang, China., Department of Emergency, 1st Affiliated Hospital, Nanjing Medical University, Nanjing, China., Department of Anesthesiology, Jiangsu Tumor Hospital, Nanjing, China., Center for Drug Discovery, College of Pharmacy, China Pharmaceutical University, Nanjing, China.

Abstract:  Oleanolic acid (OA) has been used to treat liver disorders, but whether it can attenuate hepatic ischemia-reperfusion- (IR-) associated liver dysfunction remains unexplored. In the present study, 160 male Sprague-Dawley rats were equally divided into five groups: group SH received neither hepatic IR nor drugs; group IR received hepatic IR without drugs; group CM and group OA received 0.5% sodium carboxymethylcellulose and 100 mg/kg OA, intragastrically, once a day for seven days before the hepatic IR, respectively; on the basis of treatment in group OA, group OA+wortmannin further received 15 μg/kg of PI3K inhibitor wortmannin, intraperitoneally, 30 min before the hepatic IR. Then each group was equally divided into four subgroups according to four time points (preoperation, 0 h, 3 h, and 6 h after reperfusion). Serum ALT activity, IL-1β concentration, and hepatic phosphorylation of PI3K, Akt, and GSK-3β protein expression were serially studied. We found that OA pretreatment improved histological status and decreased serum ALT and IL-1β levels. It also increased p-PI3K, p-Akt, and p-GSK-3β protein expression at all the four time points. Prophylactic wortmannin partially reversed OA's protective effects. The data indicate that OA pretreatment protects liver from IR injury during the acute phase partially through PI3K/Akt-mediated inactivation of GSK-3β. 

Retracted: No

PubPeer comments:
 figure 5a and figure 6a ![file] , 
 Retraction, April 2022: https: www.hindawi.com journals mi 2022 9825785  Mediators of Inflammation has retracted the article titled u201cProtective Effects of Pretreatment with Oleanolic Acid in Rats in the Acute Phase of Hepatic Ischemia-Reperfusion Injury: Role of the PI3K Akt Pathwayu201d [1] due to errors identified with the figures. Following the publication of the article, concerns have been identified as originally raised on Pubpeer [2]: The concerns are related to Figures 4, 5 and 6: * (i)tIn Figure 4(a), 6u2009h p-PI3K displays a high amount of similarity with 3u2009h p-PI3K after narrowing. Additionally, the 0u2009h p-PI3K bands are also highly similar to the Prep p-PI3K bands after narrowing * (ii)tIn Figure 5(a), the Prep AKT panel and the Prep GSK-3u03b2 panel in Figure 6(a) appear similar * (iii)tIn Figure 6(a), the 0u2009hr p-GSK-3ud835udefd and Prep p-GSK-3ud835udefd of 6A are highly similar The authors do not agree to the retraction, which has been agreed with the editorial board. This notice replaces the corrigendum for the same issue [3], which was published in error. ,