The relationship between the expression of TAM, survivin and the degree of necrosis of the tumor after cisplatin treatment in osteosarcoma
Authors: G Chen
Journal: European review for medical and pharmacological sciences (2017)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: TAM; survivin; necrosis; cisplatin treatment; osteosarcoma
Affiliations: Department of Orthopedics, First Affiliated Hospital of South-West Medical University, Luzhou, Sichuan Province, China. .

Abstract:  Objective: To explore the relationship between the expression of TAM, survivin and the degree of necrosis of the tumor after cisplatin treatment in osteosarcoma. Materials and methods: The mice model of osteosarcoma S180 were injected with 6 mg/kg/day of cisplatin (observation group) or the same amount of normal saline (control group) for 4 weeks. Mice were sacrificed at days 1, 4, 9, 14, 18, 22 and 28, respectively, 24 h before administration of the drug or saline, and tumor tissues were collected. The size of the tumor samples was measured and the correlation of TAM, survivin expression in osteosarcoma and necrosis degree of tumor tissue after cisplatin treatment was studied using various methods including fluorescence quantitative PCR, enzyme linked immunosorbent assay (ELISA), Western blotting and immunohistochemistry. Results: Fluorescence quantitative PCR showed that the expression of TAM, survivin mRNA in the control group was significantly higher than that in the observation group. Also, the ELISA monitoring showed that the expression of mice TAM, survivin protein in vivo was significantly lower than TAM, survivin protein expression of mice in vivo in the observation group (2.3 µg/l, 1.6 µg/l) relatively to the control group (9.7 mg/l, 10.3 µg/l). Consistent with the Western blot data, ELISA results showed that the expression of survivin and TAM protein decreased gradually with the prolongation of drug treatment along the time in the observation group. The volume and weight of the tumor in the observation group were significantly less than that of the control group. Additionally, the tumor necrosis of mice in the observation group was more significant, suggesting that the meant of the size of tumor tissue decreased significantly with the extension of the time of drug treatment. Immunohistochemical results showed that the rate of the positive cell of TAM and survivin in the observation group (82.3%) was significantly higher (p&lt;0.05) than that in the control group (19.5%). However, the rate of the positive cell of survivin and TAM gradually declined at the level of the trend with the extension of the time of drug treatment in the observation group. Conclusions: Cisplatin treatment can inhibit the expression of TAM and survivin in osteosarcoma tissue sand then, promote the necrosis of tumor tissue. 

Retracted: No

PubPeer comments:
 2019 retraction. http: www.oncotarget.com index.php?journal=oncotarget page=article op=view path%5B%5D=27064 In August 2018 an investigation of this article was requested by the Supervisory Committee of the National Natural Science Foundation of China, after allegations of fraud. The Oncotarget editorial staff also requested that Shandong University bring the paper to the attention of an ethical committee in August 2018, after the corresponding authors requested retraction on the grounds of statistical errors. The institutionu2019s investigation determined that u201call the experiments of this paper were entrusted by the first author Yu Xinshuang to a commercial company, and the original experimental data were unable to be provided. Corresponding authors and other authors were unaware of the relevant experiments nor the submission process of the paper.u201d Based on these results, all authors have agreed to the retraction of this article from Oncotarget. Original article: Oncotarget. 2016; 7:76827u201376839. DOI: https: doi.org 10.18632 oncotarget.12718. , 
 We are left to speculate about the cause of the accusations and the investigations. A cursory glance at the paper inspires my curiosity about Figures 4A ( MDC fluorescence intensity of NCI-H446 and H69 cells after treated with different doses of tunicamycin for 24 h ) and 8A ( flow cytometry of NCI-H446 CDDP and H69 CDDP cells after treated with 5 ug mL of tunicamycin or and 64 u03bcM of BEZ235 for 24 h ). ![file] In each row, a single cell-count histogram is repeated four or five times, with different vertical and horizontal scales. The authors did not receive full value from the commercial company to which they outsourced the experiments. , 
 Note: This article has been retracted. Please see the link below: https: www.oncotarget.com article 27064 , 
 In further evidence that the author did not get value-for-money from the outside laboratory where the experiment was ostensibly performed, here is Fig 3A,  the expressions of PI3K AKT mTOR signaling pathway-related proteins in NCI-H446 cells after treated with different doses of tunicamycin for 24 h . ![file] For comparison with Figs 7C and Fig 9A from [Song et al (2016)] :  Bcl-2, Bax and Caspase-3 protein expressions detected by western blotting ;  Western blot analysis of p-ASK1, p-JNK and p-p38 levels in rat myocardial cells . ![file] ![file] ,