Overexpression of lncRNA Gm2691 attenuates apoptosis and inflammatory response after myocardial infarction through PI3K/Akt signaling pathway
Authors: Dan Xu
Tingting Li
Jun Li
Yuan Guo
Jiying Chen
Anju Zuo
Hongbo Tian
Haiqing Gao
Journal: IUBMB life (2019)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: Gm2691; apoptosis; inflammation response; ischemic reperfusion injury.
Affiliations: Department of General Practice, Qilu Hospital of Shandong University, Jinan, Shandong, China., Key Laboratory of Cardiovascular Proteomics of Shandong Province, Department of Geriatric, Qilu Hospital of Shandong University, Jinan, Shandong, China., Department of Cardiology, Shandong Provincial Hospital affiliated to Shandong University, Jinan, Shandong, China.

Abstract:  Acute myocardial infarction is one of the most threatening disease in the world. In previous studies, numerous dysregulated lncRNAs exposed to ischemic reperfusion injury have been identified. In this differential lncRNAs, Gm2691 attracted our attention due to its high fold change. The aim of the study was to investigate the function and mechanism of lncRNA Gm2691 in ischemic reperfusion injury. AnaeroPack anaerobic system treated neonatal rat ventricular cardiomyocytes were used to analyze the function of lncRNA Gm2691 in vitro. Tunel, Caspase3, and inflammation markers were detected to evaluate apoptosis and inflammatory response. Rat acute myocardial infarction was performed to elucidate the function of lncRNA Gm2691 in vivo. The results showed that LncRNA Gm2691 improved the cardiac function and attenuated the inflammatory response in vivo. We also found that lncRNA Gm2691 reduced the apoptosis and improved cell survival rates in anaeroPack anaerobic system treated neonatal rat ventricular cardiomyocytes. Western blot analysis revealed that lncRNA Gm2691 decreased Akt and ERK1/2 activities, suggesting that lncRNA Gm2691 may functioned through Akt signaling pathway. We verified the function and mechanism of lncRNA Gm2691 and provide evidence that lncRNA Gm2691 may play important role in ischemic reperfusion injury, and understanding the precise role of Gm2691 will undoubtedly shed new light on the clinical treatment. 

Retracted: No

PubPeer comments:
  FIGURE 1 Overexpression of Gm2691 decreases fibrosis area and improves heart functions. (a) Representative images revealed Masson trichrome staining in the hearts isolated from four group rat hearts. ![file] Could the authors comment on the similarity of the I R and I R+Ad-con images, after a 180-deg rotation? Is it possible that two consecutive tissue slices from the same heart were accidentally assigned the wrong labels? , 
 Fig 3,  (c) Apoptosis in NRVMs in four groups was detected by TUNEL staining. Overlap marked between two conditions. ![file] , 
 #1 Dear Hoya Camphorifolia, I am writing to you in response to your inquiry about the images in our articleuff0cwith me being the corresponding author. I thank you for your interest in our work and your commitment to improving the accuracy of scientific data. They are also very important to us. After receiving your inquiries, we immediately took measures to investigate your concerns and carefully rechecked our experiment records. We found that between the I R and the I R + Ad-con groups, there was a mistake in selecting images during the process of mapping figures. Although we are sure that this mistake has no impact on our study results, we apologize for the inconvenience caused to you and other researchers. We will also contact the editorial office of the journal to make timely corrections. Through this valuable learning opportunity, we will do better in the accuracy and integrity of data in future study. Yours sincerely, Haiqing Gao , 
 #3 Many thanks! ,