Effects of TRPC1 on epithelial mesenchymal transition in human airway in chronic obstructive pulmonary disease
Authors: Li Li
Chao-Nan Ma
Xiao-Chun Liu
Feng Xu
Ya-Jun Zhang
Journal: Medicine (2017)
Discipline: CLINICAL MEDICINE
Keywords: TRPC1; epithelial mesenchymal transition; COPD; E-cadherin; vimentin
Affiliations: Department of Respiration, Huaihe Hospital of Henan University College of Nursing and Health, Henan University, Kaifeng, P.R. China.

Abstract:  Background: We investigated the effects of TRPC1 on epithelial mesenchymal transition (EMT) in human airway in chronic obstructive pulmonary disease (COPD). Methods: A total of 94 patients who underwent lobectomy were selected and divided into COPD (49 cases) and control (45 cases) groups. Immunohistochemistry was applied to detect expression of E-cadherin and vimentin and TRPC1. Correlation of TRPC1 expression with E-cadherin and vimentin expression, and correlations of lung function indicators in COPD patients with expression of TRPC1, E-cadherin, and vimentin were analyzed. Human airway epithelial cells (16HBE) were used for cell experiments; and cigarette smoking extract (CSE) was adopted to establish the COPD model using TRPC1 recombinant plasmids and siRNA. Cells were assigned into the control, CSE, CSE + vector, CSE + TRPC1, CSE + si-NC, and CSE + si-TRPC1 groups. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were implemented to detect expression of TRPC1, E-cadherin, and vimentin. Results: Compared with the control group, expression of TRPC1 and vimentin significantly increased while expression of E-cadherin decreased in the COPD group, and protein expression of TRPC1 was positively correlated with the protein expression of vimentin but negatively correlated with the protein expression of E-cadherin. Patients exhibiting positive expression of TRPC1 had lower FEV1, FEV1%Pred, and FEV1/FVC, compared with the patients exhibiting negative expression of TRPC1. Compared with the control group, expression of TRPC1 and vimentin increased, whereas expression of E-cadherin decreased in the CSE, CSE + vector, CSE + TRPC1, and CSE + si-NC groups. Compared with the CSE and CSE + vector groups, the expression of TRPC1 and vimentin increased but the expression of E-cadherin decreased in the CSE + TRPC1 group. Compared with the CSE and CSE + si-NC groups, the expression of TRPC1 and vimentin decreased but the expression of E-cadherin increased in the CSE + si-TRPC1 group. No significant differences were observed among the CSE, CSE + vector and CSE + si-NC groups. Conclusion: Overexpression of TRPC1 in COPD promoted EMT process and TRPC1 may be a new and interesting focus for COPD new treatment in the future. 

Retracted: No

PubPeer comments:
 Fig 3B,  protein expression in cells determined by Western blot assay . ![file] The loading control band is versatile. It also appears in *[left]* Fig 9C from [Han, Ju Geng (2018)] *[RETRACTED]*, *[right]* 6A from [Wen et al (2018a)] and *[below]* Fig 3E from [Wen et al (2018b)] . ![] , 
 Figs 2B, 4A: ![file] Here is a montage I prepared earlier, to illustrate [the products of a papermill] . The present WB bands appear to come from the same limited gamut. Note the loading controls in particular. ![] ,