Methyl-accepting chemotaxis proteins 3 and 4 are responsible for Campylobacter jejuni chemotaxis and jejuna colonization in mice in response to sodium deoxycholate
Authors: Aihua Sun
Dexter Sun
Jie Yan
David M Ojcius
Zhifeng Li
Hongqiang Lou
Xu&#x27;ai Lin
Jinfang Zhao
Journal: Journal of Medical Microbiology (2014)
Discipline: MICROBIOLOGY
Keywords: Methyl-accepting chemotaxis proteins; Transducer-like proteins; Bacterial chemotactic signalling; Campylobacter jejuni; Sodium deoxycholate
Affiliations: Department of Acute Infectious Diseases Control and Prevention, Jiangsu Provincial Center for Disease Control and Prevention, Jiangsu Nanjing 210009, PR China, Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, PR China, Medical School of Jinhua Occupational Technique College, Jinhua, Zhejiang 321007, PR China, Molecular Cell Biology and Health Sciences Research Institute, University of California, Merced, CA 95343, USA, Faculty of Basic Medicine, Zhejiang Medical College, Hangzhou, Zhejiang 310053, PR China, Department of Neurology and Neuroscience, New York Presbyterian Hospital and Hospital For Special Surgery, Cornell University Weill Medical College, NY 10021, USA, Depatment of Clinical Laboratory, Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang, PR China, Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, PR China

Abstract:  Methyl-accepting chemotaxis proteins (MCPs), also termed transducer-like proteins (Tlps), serve as sensors in bacterial chemotactic signalling, and detect attractants and promote bacterial movement towards suitable sites for colonization. Campylobacter jejuni is a leading cause of human enteritis, but the mechanisms responsible for bacterial chemotaxis and early colonization in the jejunum of hosts are poorly understood. In the present study, we identified several types of bile and sodium deoxycholate (SDC) acting as chemotactic attractants of C. jejuni strain NCTC 11168-O in vitro, in which SDC was the most efficient chemoattractant. In mice with bile duct ligation, the wild-type strain displayed a markedly attenuated ability for colonization. Blockage of Tlp3 or Tlp4 protein with antibody or disruption of the tlp3 or tlp4 gene (Δtlp3 or Δtlp4) caused a significant inhibition of SDC-induced chemotaxis and attenuation for colonization on jejunal mucosa in mice of the bacterium. Disruption of both the genes (Δtlp3/Δtlp4) resulted in the absence of bacterial chemotaxis and colonization, while the tlp-gene-complemented mutants (CΔtlp3 and CΔtlp4) reacquired these abilities. The results indicate that SDC is an effective chemoattractant for C. jejuni, and Tlp3 and Tlp4 are the SDC-specific sensor proteins responsible for the bacterial chemoattraction. 

Retracted: No

PubPeer comments:
 Readers should become aware of a recent preprint entitled  Alternative Lengthening of Telomeres is not synonymous with mutations in ATRX DAXX that reanalyzed the data from Sieverling et al., 2020 paper, showing that  the Sieverling et al. score overestimates the proportion of ALT associated with ATRX DAXXtrunc and misclassifies ALT tumors when these mutations are absent Reference: https: www.biorxiv.org content 10.1101 2020.05.05.076125v1 ,