MicroRNA-374b inhibits migration and invasion of glioma cells by targeting EGFR
Authors: D Fan
D-S Pan
J-J Li
Z-Q Song
P Cao
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: microRNA-374b; glioma tissues; invasive ability; regulatory mechanism; qRT-PCR
Affiliations: Department of Neurosurgery, General Hospital of Northern Theater Command, Shenyang, China. .

Abstract:  Objective: To investigate the expression level of microRNA-374b in glioma tissues and its influence on the invasive ability of glioma cells. Meanwhile, the regulatory mechanism of microRNA-374b in glioma was also explored. Patients and methods: The expression level of microRNA-374b in 32 glioma tissues and para-cancerous tissues were detected by quantitative Real-time polymerase chain reaction (qRT-PCR). The relationship between microRNA-374b expression and clinical indicators of glioma was analyzed. Meanwhile, the expression of microRNA-374b in glioma cells was verified by qRT-PCR as well. Subsequently, microRNA-374b over-expression model was constructed in glioma cell lines, including U251 and U87. Next, the effect of microRNA-374b on cellular biological functions was analyzed using cell counting kit-8 (CCK-8) assay, Wound healing test and transwell invasion assay, respectively. Finally, the relationship between miRNA and epidermal growth factor receptor (EGFR) was explored. Results: QRT-PCR results showed that the expression level of microRNA-374b in glioma was significantly lower than that of adjacent tissues, and the difference was statistically significant. Compared with patients with higher expression of microRNA-374b, the occurrence rate of lymph node or distant metastasis was significantly higher in those with lower microRNA-374b expression. In addition, compared with NC group, the proliferation, invasion and migration abilities of cells in microRNA-374b mimics group was significantly decreased. Subsequently, results demonstrated that the expression of EGFR was significantly increased in glioma cells and tissues, which was negatively correlated with microRNA-374b expression. Subsequent cell recovery experiment indicated that microRNA-374b and EGFR had a mutual regulation and could affect the malignant progression of glioma all together. Conclusions: MicroRNA-374b could inhibit the invasion and migration of glioma by regulating EGFR. Moreover, the expression of microRNA-374b was significantly associated with lymph node metastasis, distant metastasis and poor prognosis. 

Retracted: No

PubPeer comments:
 *[right]* Fig 3C from [Pan et al (2019)] .  Significant negative correlation was found between miRNA-374b and EGFR expression levels . *[left]* Fig 3C from [Liu et al (2019)] .  A significant negative correlation was found between microRNA-106a expression and DAX-1 expression in BC tissues. All data points from Pan et al are also present in Liu et al. ![file] ------------------------- UPDATE. *[right]* Fig 3C from [Pan et al (2019)] .  Significant negative correlation was found between miRNA-374b and EGFR expression levels . *[left]* Fig 3D from [Yu et al (2019)] .  There was a significant negative correlation between the expression levels of SAMD12-AS1 and P53 in glioma tissues. ![file] There are so many papers in this *oeuvre* that it s hard to keep up. , 
 One more paper with a similar correlation plot: X.-Y. Niu et al., doi: 10.26355 eurrev_201908_18738, https: pubpeer.com publications B19B8F95B27B0DD55D052B6221DEAD Here is a small collage of the plots. I have highlighted some characteristic data points with colored ellipses. ![file] ,