Heterogeneous Porphyromonas gingivalis LPS modulates immuno-inflammatory response, antioxidant defense and cytoskeletal dynamics in human gingival fibroblasts
Authors: Lijian Jin
Thanuja D K Herath
Yu Wang
Richard P Darveau
Cun-Yu Wang
Chaminda J Seneviratne
Journal: Scientific Reports (2016)
Discipline: Multidisciplinary
Keywords: Periodontal disease; Porphyromonas gingivalis; Lipopolysaccharide; Human gingival fibroblasts; Proteomics
Affiliations: National Dental Centre Singapore, Singapore., Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China., School of Dentistry, University of Washington, Seattle, USA., Oral Sciences, Faculty of Dentistry, National University of Singapore, Singapore., School of Dentistry, University of California Los Angeles, Los Angeles, USA., Department of Pharmacology &Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.

Abstract:  Periodontal (gum) disease is a highly prevalent infection and inflammation accounting for the majority of tooth loss in adult population worldwide. Porphyromonas gingivalis is a keystone periodontal pathogen and its lipopolysaccharide (PgLPS) acts as a major virulence attribute to the disease. Herein, we deciphered the overall host response of human gingival fibroblasts (HGFs) to two featured isoforms of tetra-acylated PgLPS1435/1449 and penta-acylated PgLPS1690 with reference to E. coli LPS through quantitative proteomics. This study unraveled differentially expressed novel biomarkers of immuno-inflammatory response, antioxidant defense and cytoskeletal dynamics in HGFs. PgLPS1690 greatly upregulated inflammatory proteins (e.g. cyclophilin, inducible nitric oxide synthase, annexins, galectin, cathepsins and heat shock proteins), whereas the anti-inflammatory proteins (e.g. Annexin A2 and Annexin A6) were significantly upregulated by PgLPS1435/1449. Interestingly, the antioxidants proteins such as mitochondrial manganese-containing superoxide dismutase and peroxiredoxin 5 were only upregulated by PgLPS1690. The cytoskeletal rearrangement-related proteins like myosin were differentially regulated by these PgLPS isoforms. The present study gives new insight into the biological properties of P. gingivalis LPS lipid A moiety that could critically modulate immuno-inflammatory response, antioxidant defense and cytoskeletal dynamics in HGFs, and thereby enhances our understanding of periodontal pathogenesis. 

Retracted: No

PubPeer comments:
 Figure 4A and 7A. * Red boxes: The CST3 panel from Figure 4A looks similar to the TXN1 panel from Figure 7A. Could the authors please check? ![file] , 
 #1 Dear Dr. Bik, Many thanks for identifying and letting me know this surprising oversight error. As the corresponding author of this paper, I am very sorry to be unaware of this error during submitting the manuscript and after publishing the paper. I have immediately informed the first author who performed the experiments, and prepared the manuscript and figures to make a careful check on these Western blot images and dataset. We would deal with it correctly and appropriately. Kindest regards! LJ Jin , 
 Please also examine the tubulin blots , they are very similar in appearance. Different MW are indicated for them. Can the authors please comment. , 
 Thank you very much for the comments, apologies for the late reply due to current unfortunate situation, we are in touch with the journal for corrections. Take care and stay safe! , 
 January 2021 Correction, found here: https: www.nature.com articles s41598-021-81798-0  This Article contains errors. In Figure 4A, the band for CDH11 is incorrect. As a result, the Figure legend, u201cThe gene expression of CDH11, PPIA, CTSB and CST3 proteins in P. gingivalis LPS- and E. coli LPS-stimulated HGFs analysed by Western blotu201d should read: u201cThe expression of CDH11, PPIA, CTSB and CST3 proteins in P. gingivalis LPS- and E. coli LPS-stimulated HGFs analysed by Western blotu201d The correct Figure 4 and its legend appear below as Figure 1.  Furthermore, in Figure 7A, the bands for TXN1 and Tubulin are inadvertently duplicated from Figure 4Au2019s CST3 band and Tubulin band, respectively, and the band for MnSOD is incorrect. The correct Figure 7 appears below as Figure 2 I thank the authors and journal staff for correcting this error. ,