Antiapoptotic and neuroprotective role of Curcumin in Pentylenetetrazole (PTZ) induced kindling model in rat
Authors: Alka Bhatia
Amitava Chakrabarti
Dibyojyoti Banerjee
Sweta Kumari
Lekha Saha
Journal: Indian journal of experimental biology (2016)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: Kindling; Curcumin; PTZ kindled rats; Apoptosis; Neuronal damage

Abstract:  Kindling, a sub threshold chemical or electrical stimulation, increases seizure duration and enhances accompanied behavior until it reaches a sort of equilibrium state. The present study aimed to explore the effect of curcumin on the development of kindling in PTZ kindled rats and its role in apoptosis and neuronal damage. In a PTZ kindled Wistar rat model, different doses of curcumin (100, 200 and 300 mg/kg) were administrated orally one hour before the PTZ injections on alternate day during the whole kindling days. The following parameters were compared between control and experimental groups: the course of kindling, stages of seizures, Histopathological scoring of hippocampus, antioxidant parameters in the hippocampus, DNA fragmentation and caspase-3 expression in hippocampus, and neuron-specific enolase in the blood. One way ANOVA followed by Bonferroni post hoc analysis and Fischer's Exact test were used for statistical analyses. PTZ, 30 mg/kg, induced kindling in rats after 32.0 ± 1.4 days. Curcumin showed dose-dependent anti-seizure effect. Curcumin (300 mg/kg) significantly increased the latency to myoclonic jerks, clonic seizures as well as generalized tonic-clonic seizures, improved the seizure score and decreased the number of myoclonic jerks. PTZ kindling induced a significant neuronal injury, oxidative stress and apoptosis which were reversed by pretreatment with curcumin in a dose-dependent manner. Our study suggests that curcumin has a potential antiepileptogenic effect on kindling-induced epileptogenesis. 

Retracted: No

PubPeer comments:
 The Tables 1 in three of the four papers listed above also show remarkable similar values - some marked here, but several others to be found. ![file] , 
 Figure 1 in this paper looks remarkably similar to figures in three other papers by shared researchers. Thus, in total, four papers appear to show the same gel. The four papers are: * Lekha Saha et al. - Anti-kindling Effect of Bezafibrate, a Peroxisome Proliferator-activated Receptors Alpha Agonist, in Pentylenetetrazole Induced Kindling Seizure Model - Journal of Epilepsy Research 2014; 4(2): 45-54.t- DOI: 10.14581 jer.14011 - https: pubpeer.com publications 5DA39E0F9B3FECB5FB244FBF4718A4 * Lekha Saha et al. - Understanding the anti-kindling role and its mechanism of Resveratrol in Pentylenetetrazole induced-kindling in a rat model - Pharmacology, Biochemistry and Behavior 120 (2014) 57u201364 - DOI: 10.1016 j.pbb.2014.01.010 - https: pubpeer.com publications A5EAA270F953F8F1553C8E3B542D53 * Lekha Saha et al. - Gastroprotective effect of bezafibrate, a peroxisome proliferator activated receptor a agonist and its mechanism in a rat model of aspirin-induced gastric ulcer - Advances in Digestive Medicine (2016) -DOI: 10.1016 j.aidm.2016.04.001 - https: pubpeer.com publications 37BC35FD27C1AB7130178F6716C2B7 * Lekha Saha et al., - Antiapoptotic and neuroprotective role of Curcumin in Pentylenetetrazole (PTZ) induced kindling model in rat - Indian J Exp Biol . 2016 Feb;54(2):133-41 - PMID: 26934781 - https: pubpeer.com publications 18EC51EB813A08FCA5D010C358C376 Despite the different experiments, the gels look very similar to each other. In addition, in each of these, lanes 1 2 3 4 9 look remarkably similar. Yellow boxes showing the possible lane similarities are only shown in one of the four versions. ![file] ![file] ,