High LINC01605 expression predicts poor prognosis and promotes tumor progression via up-regulation of MMP9 in bladder cancer
Authors: Jie Yang
Wei Zhang
Wei Wang
Zhiqiang Qin
Ran Li
Peng Han
Qianwei Xing
Jianxin Xue
Jingyuan Tang
Chao Qin
Yi Wang
Lei Zhang
Journal: Bioscience Reports (2018)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Bladder cancer; EMT; MMP9; Migration; lncRNA LINC01605.
Affiliations: Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210009, China., Department of Urology, Jiangsu Province Hospital of TCM, Affiliated Hospital of Nanjing University of TCM, Nanjing 210029, China., Department of Urology, Affiliated Hospital of Nantong University, Nantong 226001, China  ., Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210009, China  .

Abstract:  The advent of high-throughput sequencing methods has facilitated identification of novel long non-coding RNAs (lncRNAs), which have been demonstrated to play an important role in multiple tumors. Moreover, with the assistance of bioinformatics analysis, LINC01605 has been found to be up-regulated in bladder cancer (BC) tissues compared with normal tissues. Hence, the present study was to explore its specific biological role and related mechanism in BC. The relative expression level of LINC01605 was measured in a cohort of BC tissues with matched normal tissues as well as human BC cell lines by quantitative real-time PCR (qRT-PCR). Survival analysis was performed to explore the relationship between LINC01605 expression and the prognosis of BC patients. The biological function of LINC01605 was studied in vitroand in vivo, by means of CCK-8 assay, colony formation assay, transwell assay, and tumor xenografts mice model. LINC01605 was found to be frequently highly expressed in both human BC cells and tissues. Survival analysis indicated that high LINC01605 expression was associated with higher histological grade and clinical stages. In addition, down-regulated LINC01605 in BC cells could significantly inhibit the abilities of proliferation, migration, and invasion in vitro and knockdown of LINC01605 in subcutaneous xenograft tumor model could impede tumorigenesis in vivo Mechanistically, LINC01605 could activate epithelial-mesenchymal transition (EMT) signaling pathway and promote the expression of matrix metallopeptidase (MMP) 9 (MMP9). In summary, our results shed light on that LINC01605, as a new prognostic biomarker, could promote the proliferation, migration, and invasion of BC cells via activating EMT signaling pathway and up-regulating MMP9 expression. 

Retracted: No

PubPeer comments:
 In Figure 4C, a micrograph has been duplicated and presented as something else. ![file] , 
 Dear professoruff1a Thank you for your prompt attention to our manuscript and helpful suggestions. Iu2019m writing to you in response to recent inquiries you posted with regard to the study u201cLong non-coding RNA LINC01605 predicts poor prognosis and promotes tumor progression via upregulation of MMP9 in bladder canceru201d, with me being the corresponding author. Due to the negligence of our work, incorrect picture about transwell migration of sh-lnc01605-1 in T24 cells was selected, due to no systematic folder management. Therefore, the modified picture has been attached. Moreover, we had contacted this press for article correction. Best wishes. , 
 #2 Thank you for your reply. Perhaps not critical, but figure 5C suffers from similar overlap issues as figure 4C. *** ![file] , 
 Thank you for your reply, Qianwei Xing.  no systematic folder management doesn t sound promising. How can you be sure about any data without control of your folders? The last update by Actinopolyspora Biskrensis is reason to be more concerned... , 
 #4 I think it s euphemism or wrong translation, and the real meaning is to apologize for the confusion of file naming. , 
 The authors of the original article u201cHigh LINC01605 expression predicts poor prognosis and promotes tumor progression via upregulation of MMP9 in bladder canceru201d (Bioscience Reports (2018) 38(5); https: doi.org 10.1042 BSR20180562) would like to provide a correction to Figure 4 of their published paper. Due to a management error, an incorrect transwell migration image of sh-lnc01605-1 in T24 cells was included. The corrected Figure 4 is shown in this correction article. *** ![file] , 
 Appears to be additional image overlaps in Figure 4B and 5B and 5C. *** 4B ![file] *** 5B C ![file] , 
 April 6, 2021 u201cThis Retraction follows an Expression of Concern relating to this article previously published by Portland Press. This article is being retracted from Bioscience Reports at the request of the Editor-in-Chief and the Editorial Board following receipt of a notification from a reader, alerting the Editorial Board to two pairs of duplicated images, one in Figure 5C and the other in Figure 4C (previously corrected). The Editorial Office has also identified a third pair of duplicated images between Figures 4B and 5B. The authors were contacted regarding the concerns raised and requested to correct the paper, however given the quantity of duplications within the article the Editorial Board stands by the decision to retract. The authors agree to the retraction.u201d I appreciate the rapid decision by the editors. ,