Plasma exosomes from endometrial cancer patients contain LGALS3BP to promote endometrial cancer progression
Authors: Yuan Tan
Xiaoping Wan
Kai Wang
Xiang Hu
Yunfeng Song
Mengfei Wang
Huan Tong
Journal: Oncogene (2021)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Endometrial cancer; Exosomes; LGALS3BP; Angiogenesis; Proliferation
Affiliations: Department of Gynaecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, PR China., Clinical and Translational Research Centre, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, PR China. ., Department of Gynaecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, PR China. .

Abstract:  Endometrial cancer (EC) is a common gynaecological cancer worldwide. Exosomes, secreted by living cells and detected in various body fluids, can exchange information between organs and compartments to affect cellular functions, such as proliferation, apoptosis, migration and angiogenesis. We hypothesise that plasma exosomal contents are altered during cancer progression and promote cancer growth and angiogenesis by delivering biomolecules to cancer and vascular endothelial cells. In this study, circulating exosomes derived from EC patients and age-matched healthy people were acquired by commercial kits. Cell counting kit-8, Transwell and Matrigel tube formation assays showed that circulating exosomes from EC patients promote EC cell growth and human umbilical vein endothelial cell (HUVEC) angiogenesis. Next, proteomic analysis and ELISA revealed that plasma exosomal lectin galactoside-binding soluble 3 binding protein (LGALS3BP) increased during EC progression. Moreover, to explore the function of exosomal LGALS3BP, we acquired exosomes containing high levels of LGALS3BP by overexpressing LGALS3BP in human embryonic kidney 293 cells, and we demonstrated that highly contained exosomal LGALS3BP contributed to EC cell proliferation and migration and HUVEC functions via the activation of the PI3K/AKT/VEGFA signalling pathway both in vitro and in vivo. Finally, high LGALS3BP expression was observed in human EC tissue, which indicated a poor prognosis. In addition, immunohistochemical analysis of human EC tissues revealed that LGALS3BP expression was correlated with VEGFA expression and blood vessel density. Hence, we proposed that plasma exosomes containing LGALS3BP contributed to EC growth and angiogenesis during EC progression, which also provided a novel perspective on EC diagnosis and prognosis. 

Retracted: No

PubPeer comments:
 2018 correction. https: mcr.aacrjournals.org content 16 1 184.long In this article (Mol Cancer Res 2012;10:821u201333), which was published in the June 2012 issue of Molecular Cancer Research (1), the authors reported erroneous immunoblot exposures. Specifically, the blot labeled u201cu03b2-actinu201d in Figure 1C contained an erroneous Akt1 exposure rather than a GAPDH exposure, and the blot labeled u201cCyclin D2u201d in Figure 2A contained an erroneous Cyclin D1 exposure rather than a Cyclin D2 exposure. The corrected blots depicting GAPDH and Cyclin D2 exposures are shown below. ,