Downregulation of microRNA-586 Inhibits Proliferation, Invasion and Metastasis and Promotes Apoptosis in Human Osteosarcoma U2-OS Cell Line
Authors: Lei Yang
Zong-Ming Liu
Shao-Qian Cui
Yan-Wei Rao
Xiao-Jing Jia
Huan Wang
Journal: Cytogenetic and Genome Research (2015)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: microRNA-586; osteosarcoma; cell proliferation; apoptosis; invasion
Affiliations: Department of Radiology, Jilin Cancer Hospital, Changchun, PR China.

Abstract:  In this study, we aim to examine the association of microRNA-586 (miR-586) with osteosarcoma (OS) cell proliferation, apoptosis, invasion, and metastasis. U2-OS cell lines were divided into 4 groups: an miR-586 group, anti-miR-586 group, control group (empty plasmid) and blank group (no plasmid). qRT-PCR was used to detect miR-586 expression, cell counting kit-8 and EdU assays to detect cell proliferation, flow cytometry to detect cell cycle distribution, Annexin V/PI double staining to detect cell apoptosis, and the Transwell assay to detect cell invasion and metastasis. miR-586 expression was significantly higher in the miR-586 group but significantly lower in the anti-miR-586 group compared with the control and blank groups. Cell proliferation at 2-5 days after cell transfection and the EdU-positive cell number increased obviously in the miR-586 group but decreased clearly in the anti-miR-586 group. In the miR-586 group, cells at G0/G1 stage and apoptosis cells significantly decreased, while cells at G2/M and S stages and invasive and metastatic cells significantly increased compared to the control and blank groups; however, opposite trends were found in the anti-miR-586 group. Downregulation of miR-586 expression in OS may inhibit cell proliferation, invasion and metastasis, and promote cell apoptosis. 

Retracted: Yes

PubPeer comments:
  Fig. 9. Transwell assay detecting cell invasion and metastasis in U2-OS cells. ![file] Evidently the overlap between the Blank and Control Invasion results (green rectangles) was not an issue for the reviewers. Nor was the overlap between Invasion and Migration results for miR-586 (yellow rectangles). Setting those overlaps aside, here I have marked up Fig 9 to show the overlaps with panels of Fig 7A from [Xiong et al (2018)] . ![file] ![file] , 
 Fig. 6.  EdU assay detecting cell proliferation. Fluorescence microscope detecting the fluorescence intensity of EdU cells in S phase (left): EdU-positive cells (red) and Hoechst 33342 (blue) . ![file] All four columns also appear in Fig. 8A, C of [He et al (2016)] , albeit in monochrome.  Edu was used to detect the proliferation of U2OS (A, B) and MG63 (C, D) cells in each group. (A, C) fluorescence intensity of Edu cells in U2OS (A) and MG63 (C) cells in S phase under fluorescence microscope, Edu positive cells presented white lights, top row, nucleus stained by Hoechst33342 presented white lights, bottom two rows . ![file] No authors in common. Different institutions. , 
 Retracted [December 2021] . --------------------- After the publication of this article, concerns were raised about the integrity of some of the data presented. Specifically, there is image duplication within Figure 9, and elements of Figures 6 and 9 in this article are presented in other articles without justification. The authors did not respond to requests to comment on the concerns and to provide the raw data within the given time frame despite multiple attempts of contact. The matter was raised to the corresponding authoru2019s institution who did not respond to our request for an investigation. The corresponding author subsequently requested the retraction of this article without responding to the concerns raised. The authors have not responded to our requests to elaborate on the reason for requesting the retraction of the article despite multiple attempts of contact. Given the severity of the concerns raised this article is being retracted. The authors have not responded to our correspondence regarding this retraction despite multiple attempts of contact. ,