<p>microRNA-605 directly targets SOX9 to alleviate the aggressive phenotypes of glioblastoma multiforme cell lines by deactivating the PI3K/Akt pathway</p>
Authors: Meifeng Yin
Yongdong Liu
Jing Wang
Jianwu Jia
Journal: OncoTargets and Therapy (2019)
Discipline: CLINICAL MEDICINE
Keywords: SRY-Box 9; glioblastoma multiforme; malignant phenotypes; microRNA-605.
Affiliations: Department of Neurosurgery, Yidu Central Hospital of Weifang, Weifang, Shandong 262500, People's Republic of China., Department of Pediatrics, Weifang People's Hospital, Weifang, Shandong 261041, People's Republic of China.

Abstract:  Background: Aberrant microRNA (miRNA) expression has been widely reported to play a crucial role in the progression and development of glioblastoma (GBM). miR-605 has been identified as a tumor-suppressing miRNA in several types of human cancers. Nevertheless, the expression profile and detailed roles of miR-605 in GBM remain unclear and need to be further elucidated. Materials and methods: RT-qPCR analysis was utilized for the determination of miR-605 expression in GBM tissues and cell lines. In addition, CCK-8 assay, transwell migration and invasion assays, as well as sub-cutaneous xenograft mouse models were utilized to evaluate the effects of miR-605 upregulation in GBM cells. Notably, the potential mechanisms underlying the activity of miR-605 in the malignant phenotypes of GBM were explored. Results: We observed that expression of miR-605 was reduced in GBM tissues and cell lines. Decreased miR-605 expression exhibited significant correlation with KPS score. The overall survival rate in GBM patients with low miR-605 expression was lower than that of patients with high miR-605 expression. Increased miR-605 expression suppressed the proliferation, migration, and invasion of U251 and T98 cells. In addition, miR-605 upregulation impaired tumor growth in vivo. Furthermore, SRY-Box 9 (SOX9) was identified as a direct target gene of miR-605 in U251 and T98 cells. SOX9 expression was shown to exhibit an inverse correlation with miR-605 expression in GBM tissues. Moreover, silencing of SOX9 expression mimicked the tumor-suppressing roles of miR-605 in U251 and T98 cells, while SOX9 restoration rescued the suppressive effects of miR-605 overexpression in the same. Notably, miR-605 suppressed the PI3K/Akt pathway in GBM in vitro and in vivo. Conclusion: These results demonstrated that miR-605 acts as a tumor suppressor in the development of GBM by directly targeting SOX9 and inhibiting the activation of the PI3K/Akt pathway, suggesting its potential role as a therapeutic target for GBM. 

Retracted: Yes

PubPeer comments:
 This review paper appears to be unexpectedly similar to a 2014 PhD thesis by Marlen Stacey Chawani at the University of Oslo, Norway, called  Development of Electronic Medical Record Systems for Maternal Health Services in Rural Settings . That 2014 thesis can be found here: https: www.mn.uio.no ifi english research networks hisp research-library thesis chawani2014.pdf Here is a marked-up version of the text by Zhang and Zhang 2016, with highlighted text showing similarities to the 2014 thesis. Generated in SimTexter : ![file] ![file] ,