LncRNA SNHG12 accelerates the progression of ovarian cancer via absorbing miRNA-129 to upregulate SOX4
Authors: D Sun
X-H Fan
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: lncRNA; SNHG12; miRNA-129; ovarian cancer; proliferation
Affiliations: Department of Obstetrics and Gynecology, Hanchuan People's Hospital, Hanchuan, China. .

Abstract:  Objective: To clarify whether long non-coding RNA (lncRNA) SNHG12 could regulate the proliferative and migratory abilities of ovarian cancer (OC) cells through mediating microRNA-129 (miRNA-129), thus influencing the progression of OC. Patients and methods: The expression patterns of SNHG12 and miRNA-129 in OC tissues and adjacent normal tissues were determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Meanwhile, their expression levels in OC cell lines were also examined. Regulatory effects of SNHG12 and miRNA-129 on the proliferative and migratory abilities of OC cells were evaluated by cell counting kit-8 (CCK-8) and transwell assay, respectively. Through the dual-luciferase reporter gene assay, we explored the binding between miRNA-129 with SNHG12 and SOX4. A series of rescue experiments were conducted to clarify the role of SNHG12/miRNA-129/SOX4 regulatory loop in the progression of OC. Results: SNHG12 was upregulated in OC tissues relative to adjacent normal ones. Patients with metastatic OC or those in stage III-IV had a higher level of SNHG12 compared with non-metastatic or stage I-II patients. The 5-year survival was markedly worse in OC patients with high-level SNHG12 than those in the low-level group. Similarly, SNHG12 was highly expressed in OC cell lines. Overexpression of SNHG12 accelerated A2780 and HO8910 cells to proliferate and migrate. We observed the binding between SNHG12 and miRNA-129, and the latter was lowly expressed in OC. The miRNA-129 overexpression partially reversed the promotive effects of SNHG12 on proliferative and migratory abilities of OC cells. Subsequently, SOX4 was proved to be the target gene of miRNA-129. The SOX4 overexpression was further confirmed to reverse the inhibitory effects of miRNA-129 on proliferative and migratory abilities of OC cells. Conclusions: SNHG12 accelerates the proliferative and migratory abilities of OC cells via sponging miRNA-129 to upregulate SOX4. 

Retracted: No

PubPeer comments:
 Clockwise from upper left: a. Fig 1D from [Jiang et al (2019)] .  Lnc00908 was highly expressed in OC... Correlation between five-year survival rate of OC patients and lnc00908 expression . b. Fig 1D from [Zhao Huang (2019)] .  SNHG14 was highly expressed in ovarian cancer... The five-year survival rate was analyzed . c. Fig 2B from [Sheng et al (2019)] .  CircUBAP2 promoted OC development... A negative correlation was found between the five-year survival rate of OC patients and circUBAP2 expression . **d. Fig 1D from [Sun Fan (2019)] .  Upregulation of SNHG12 in OC. ... The survival analysis demonstrated a worse prognosis in OC patients with high-level SNHG12 relative to those with low-level SNHG12 .** ![file] Many similarities, most notably between b) and c), and between a) and d). ,