miR-448 suppresses proliferation and invasion by regulating IGF1R in colorectal cancer cells
Authors: Bai Li
Zhihong Li
Liang Ge
Lei Wang
Minghe Li
Journal: American journal of translational research (2016)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: IGF1R; colorectal cancer; invasion; miR-448; proliferation.
Affiliations: Department of Colorectal and Anal Surgery, The First Hospital of Jilin University Changchun 130021, China., Department of Anesthesiology, The First Hospital of Jilin University Changchun 130021, China., Department of Oral and Maxillofacial Surgery, School of Stomatology Hospital of Jilin University Changchun 130021, China., Department of Thoracic Surgery, The First Hospital of Jilin University Changchun 130021, China.

Abstract:  Accumulating evidence has demonstrated that miR-448 expression was downregulated, and exerted tumor suppressor roles in several types of cancer. However, the biological function and underlying mechanism of miR-448 in colorectal cancer (CRC) have not been elucidated. In this study, we detected the miR-448 expression in CRC tumor tissues and adjacent normal tissues (ANT) and five colorectal cancer cell lines by real time quantitative RT-PCR (qRT-PCR). Cell proliferation, colony formation, migration and invasion were investigated in CRC cells transfected miR-448 mimic or negative control mimic by MTT, colony forming, wound healing and transwell invasion assays, respectively. Target gene was identified by bioinformatic prediction, dual-luciferase reporter assay, qRT-PCR and Western blot. Our data proved that miR-448 expression was downregulated in CRC tissues and cell lines, and was inversely associated with advanced tumor-node-metastasis (TNM) stage (P &lt; 0.01), and lymph node metastasis (P &lt; 0.01). Overexpression of miR-448 suppressed CRC cell proliferation, colony formation, migration, and invasion. Moreover, we identified insulin-like growth factor 1 receptor (IGF1R) as a direct target gene of miR-448 in CRC cell. IGF1R expression was upregulated in CRC tissues and cell lines, and its expression was negatively correlated with the expression level of miR-448 in CRC tissues(r = -0.569, P = 0.002). In addition, IGF1R overexpression rescued the suppressive effect of miR-448-mediated CRC on cell proliferation, colony formation, migration and invasion. These results suggested that miR-448 might serve as a tumor suppressor in CRC partly through targeting IGF1R. 

Retracted: Yes

PubPeer comments:
 *[left]* Fig 5B.  IGF1R expression on ... protein level (B) was detected in CRC tissues (T) and the matched adjacent normal tissues (ANT). GAPDH was used to serve as the loading control . *[right]* Fig 6A from [Liu et al (2016)] . ![file] The loading control is the same (see [e.g. here] for a partial list of its other appearances). Lanes in the substantive band also match, after a 180 deg flip. , 
 Fig 5D.  Western blotting analysis of IGF1R protein expression in five CRC cell lines (HCT116, HT29, SW480, SW620 and LoVo) and normal colonic cell line (NCM460) . ![file] The two left-hand lanes of the loading control match the right-hand lanes of frequently-appearing loading control  5 considered [e.g. here] . ![] , 
 Retracted [15 January 2022] .  Falsified materials and or data reported in this publication had been detected by a knowledgeable reader and subsequently confirmed by further investigation of the editorial office. Therefore, the entire article has been retracted in accordance with this journalu2019s policy and editorial decision. ,