Globularifolin exerts anticancer effects on glioma U87 cells through inhibition of Akt/mTOR and MEK/ERK signaling pathways in vitro and inhibits tumor growth in vivo
Authors: Fei Wang
Hang Li
Yunhu Yu
Xiaohong Fu
Qishan Ran
Kaihua Yang
Yuanchao Wen
Journal: Biochimie (2017)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: Akt/mTOR; Apoptosis; Cell cycle arrest; Glioma; Globularifolin.
Affiliations: Department of Neurosurgery, The First People's Hospital of Zunyi, Department of Neurosurgery, ZunYi, 563000, China. Electronic address: ., Department of Neurosurgery, The First People's Hospital of Zunyi, Department of Neurosurgery, ZunYi, 563000, China., Department of Cerebrovascular Disease, The First Affiliated Hospital of Zunyi Medical College, Zunyi, 563000, China.

Abstract:  This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of the Editor-in-Chief. Reason for retraction: Figures 5 and 7B show shared images with Figures 4 and 7 of a publication in Mol Med Reports, Zeng et al. (2018) 10.3892/mmr.2018.8599. The two manuscripts were submitted from different laboratories at almost exactly the same time. 

Retracted: No

PubPeer comments:
 *[right]* Figure 5 from [Yu et al (2017)] .  Expression of apoptosis-regulatory proteins in glioma U87 cells at indicated concentrations of globularifolin . *[left]* Figure 4 from [Zeng et al (2018)] .  Effect of CAE on the protein expression of Bax and Bcl-2 was determined using western blot analysis ![file] , 
 *[right]* Figure 7B from [Yu et al (2017)] .  Inhibition of cell migration and invasion of glioma cells at indicated concentrations of globularifolin. ... (B) globularifolin inhibited glioma U87 cell migration and invasion as tested by transwell assays. The migratory and invasive cells were detected by crystal violet staining and photographed . *[left]* Figure 7 from [Zeng et al (2018)] .  Effects of CAE on the migration of A431 cells at indicated concentrations ![file] , 
 #1 More sightings. *[left]* Figure 6 from [Niu et al (2018)] .  Effect of cycloartenol on the Bax and Bcl-2 expression in glioma U87 cells as shown by western blotting . *[right]* Figure 7 from [He et al (2018)] .  Western blot analysis of the levels of mTOR P13K Akt pathway proteins showing that arglabin caused dose-dependent inhibition of p-mTOR, p-PI3K and p-AKT proteins. ![file] , 
 *[left]* Figure 4A from [Yu et al (2017)] .  Effect of indicated doses of globularifolin on apoptosis (A) DAPI staining . *[right]* Figure 3 from [Deng Li (2019)] .  Effect of linderalactone on the apoptosis of A549 cells as determined by DAPI staining . ![file] UPDATE. Another sighting! Fig 2A from [Wang et al (2020)] .  Sugiol induces apoptosis in SKOV3 cells as depicted by DAPI staining (A), AO EB staining . ![] , 
 *[right]* Figure 4B from [Yu et al (2017)]  Effect of indicated doses of globularifolin on apoptosis ...(B) Estimation of apoptotic glioma U87 cell populations at indicated doses by annexin V PI using flow cytometry . *[left]* Figure 5 from [Zheng et al (2019)] .  Estimation of the apoptotic cell populations in Trifolirhizin treated SNU-5 cells as depicted by annexin V PI staining. The Figure shows that the percentage of apoptotic SNU-5 cells increased with the increase in the concentration of Trifolirhizin. ![file] , 
 *[right]* Figure 6 from [Yu et al (2017)] .  Effect of indicated concentration the key proteins of Akt mTOR and MEK ERK signalling pathways. *[left]* Figure 8 from [Zheng et al (2019)] .  Effect of Trifolirhizin on the mTOR PI3K KT signalling pathway as depicted by western blot analysis. The Figure shows that Trifolirhizin blocked the PI3K AKT mtTOR signaling pathway dose-dependently . ![file] , 
 #5 Fig 4B again *[right]*: ![] For comparison: Figure 6 from [Wu Li (2017)] .  Estimation of apoptotic MCF-7 cell populations at indicated doses of GA by annexin V PI and followed by flow cytometry . ![] ,