Long noncoding RNA SNHG16 acts as an oncogene in Wilms' tumor through sponging miR-200a-3p
Authors: C-Y Dong
C Zhang
N Tao
X-S Zhao
C-M Gong
Journal: European review for medical and pharmacological sciences (2020)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: lncRNA; SNHG16; Wilms' tumor; RT-qPCR; cell migration
Affiliations: Department of Pediatric Surgery, The First Hospital of Jilin University, Changchun, China. .

Abstract:  Objective: Recently, the role of long noncoding RNA (lncRNAs) in tumor progression has attracted much attention. The aim of this study was to investigate the role of lncRNA SNHG16 in the development of Wilms' tumor, and to explore the underlying mechanism. Patients and methods: Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was used to detect SNHG16 expression in Wilms' tumor patients' tissues. Function assays, including wound healing assay, and transwell assay, were conducted to detect the changes of biological behaviors in Wilms' tumor cells due to gain or loss of SNHG16. Besides, the luciferase reporter gene assay was performed to explore the underlying mechanism. Results: The expression level of SNHG16 was significantly up-regulated in Wilms' tumor tissues when compared with adjacent tissues. Cell migration and invasion abilities were significantly repressed via down-regulation of SNHG16. However, opposite results were obtained after up-regulation of SNHG16 in vitro. After the down-regulation of SNHG16, the expression of miR-200a-3p increased significantly. However, the expression of miR-200a-3p was remarkably reduced via up-regulation of SNHG16 in vitro. Furthermore, SNHG16 acted as a competing endogenous RNA via sponging miR-200a-3p in Wilms' tumor. Conclusions: SNHG16 induced the metastasis of Wilms' tumor via sponging miR-200a-3p. Our findings might provide a new prospect for the diagnosis and therapy of Wilms' tumor. 

Retracted: Yes

PubPeer comments:
 This paper is one of a set of 121 papers that all contain images from the same library of about 100 photos and plots. This is unexpected, because each paper has different authors and different affiliations, with little to no overlap, and describes different cancer types and molecules. It would be very hard to explain how authors from different labs would all obtain the same photo. Therefore, the papers might have all be derived from the same source, such as a paper mill that sells ready-to-be-published papers. This  Stock Photo paper set has been described in more detail on my blog post, https: scienceintegritydigest.com 2020 07 05 the-stock-photo-paper-mill and in a Wall Street Journal article by Eva Xiao (currently behind a paywall): https: www.wsj.com articles chinese-research-papers-raise-doubts-fueling-global-questions-about-scientific-integrity-11593939600 The vast majority (113 121) of the papers were published in the same journal, European Review for Medical and Pharmacological Sciences (ERMPS). A link to the full set of papers can be found here: https: docs.google.com spreadsheets d 172RWS18w1lTNJ9Nnj80fuXXTqTm4fGkoK0sdYyvKE3g edit?usp=sharing All papers have been reported to the journal editors on July 2, 2020. , 
 https: www.europeanreview.org article 22219 Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cLong noncoding RNA SNHG16 acts as an oncogene in Wilmsu2019 tumor through sponging miR-200a-3p, by X.-S. Zhao, N. Tao, C. Zhang, C.-M. Gong, C.-Y. Dong, published in Eur Rev Med Pharmacol Sci 2020; 24 (8): 4145-4151u2013DOI: 10.26355 eurrev_202004_20994u2013PMID: 32373950u201d has been withdrawn. The Publisher apologizes for any inconvenience this may cause. , 
 The primers for CREB1 in the article  F: CCCAAGCTTATGACCATGGAATCTGGAGC R: TGCTCTAGATAATCTGATTTGTGGCAGTAAAGG cannot get any human genes. Again, the primers for microRNA-155-5pin the article cannot get targeted miRNA . ,