MicroRNA-497 suppresses osteosarcoma tumor growth in vitro and in vivo
Authors: Zhihong Li
Liang Ge
Liang Niu
Minghe Li
Baisong Zheng
Journal: Oncology Letters (2016)
Discipline: CLINICAL MEDICINE
Keywords: miR-497; microRNAs; osteosarcoma; tumor growth.
Affiliations: Department of Anesthesiology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China., Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China., Department of Oral and Maxillofacial Surgery, School of Stomatology Hospital of Jilin University, Changchun, Jilin 130000, P.R. China., Operating Room, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China., Department of Thoracic Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.

Abstract:  It has been demonstrated that microRNA-497 (miR-497) acts as a tumor suppressor and is involved in tumor progression, development and metastasis in several types of cancer. However, little is known about the exact role of miR-497 in osteosarcoma (OS). The aim of the current study was to investigate the potential role of miR-497 in human OS. The role of miR-497 in the growth and survival of OS cells was determined using several in vitro approaches and a nude mouse model. The results demonstrated that exogenous expression of miR-497 in human OS MG63 cells suppressed cell proliferation, colony formation, migration and invasion, and induced cell apoptosis and cell arrest at the G0/G1 phase of the cell cycle. In addition, the results of the in vivo study indicated that restoration of miR-497 inhibited OS tumor growth in a nude mouse model. Overall, the results of the present study identified a crucial tumor suppressive role of miR-497 in the progression of OS, and suggested that miR-497 may be a potential therapeutic agent for the treatment of OS. 

Retracted: No

PubPeer comments:
 *[left]* Fig 3C.  Western blot analysis of uPA, MMP-2 and MMP-9 protein expression following transfection with miR-497 mimic or miR-control. GAPDH was used as an internal control . *[right]* Figs 3C,E from [Liu et al (2015)] .  The ... (C) protein expression levels of PTEN were detected in the HCC cells after transfection with miR-494 mimic or inhibitor. The PTEN protein expression level was detected in... (E) HCC cell lines by western blotting. I have concerns. ![file] , 
 Fig 4A.  **miR-497** inhibits tumor growth in vivo. (A) Representative image of tumors formed in BALB c xenograft mouse models at 28 days following injection with human osteosarcoma **MG63** cells transfected with a miR-497 mimic or miR-control. ![file] Fig 6 from [Zhang et al (2016)] .  **miR-22** suppresses tumor growth in a **768-O** xenograft model. (A) Photographs of tumor tissue collected after sacrifice at day 30 . ![file] UPDATE: Fig 6B fro [Rao et al (2017)] .  **miR-122** suppresses xenograft tumor growth in vivo by targeting CREB1. ... B. The mice were killed at 30 days after inoculation, and **[SGC-7901]** tumor tissues was excised and imaged. ![file] Travelling tumors. -------------------------------------- The earliest sighting so far was as Fig 5A of [Li et al (2015)] .  Representative images of the tumors in the different treatment group . ![file] ,