Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK axis
Authors: Tianxin Lin
Wei Dong
Junming Bi
Jian Huang
Juanyi Shi
Meihua Yang
Hongwei Liu
Ning Jiang
Journal: Molecular Cancer (2018)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Bladder cancer; G3BP2; SRC/FAK; circFNDC3B; miR-1178-3p.
Affiliations: Department of Urology and Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107th Yanjiangxi Road, Yuexiu District, Guangzhou, 510120, China., Department of Urology and Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107th Yanjiangxi Road, Yuexiu District, Guangzhou, 510120, China. .

Abstract:  Background: Increasing evidence has revealed that circular RNAs (circRNAs) play crucial roles in cancer biology. However, the role and underlying regulatory mechanisms of circFNDC3B in bladder cancer (BC) remain unknown. Methods: A cell invasion model was established by repeated transwell assays, and invasion-related circRNAs in BC were identified through an invasion model. The expression of circFNDC3B was detected in 82 BC tissues and cell lines by quantitative real-time PCR. Functional assays were performed to evaluate the effects of circFNDC3B on proliferation, migration and invasion in vitro-, and on tumorigenesis and metastasis in vivo. The relationship between circFNDC3B and miR-1178-3p was confirmed by fluorescence in situ hybridization, pull-down assay and luciferase reporter assay. Results: In the present study, we identified a novel circRNA (circFNDC3B) through our established BC cell invasion model. We found that circFNDC3B was dramatically downregulated in BC tissues and correlated with pathological T stage, grade, lymphatic invasion and patients' overall survival rate. Functionally, overexpression of circFNDC3B significantly inhibited proliferation, migration and invasion both in vitro and in vivo. Mechanistically, circFNDC3B could directly bind to miR-1178-3p, which targeted the 5'UTR of the oncogene G3BP2. Moreover, circFNDC3B acted as a miR-1178-3p sponge to suppress G3BP2, thereby inhibiting the downstream SRC/FAK signaling pathway. Conclusions: CircFNDC3B may serve as a novel tumor suppressive factor and potential target for new therapies in human BC. 

Retracted: No

PubPeer comments:
 Figure 8e has a duplicate image ![file] , 
 #1 Dear Dr. Dendrodoa Grossularia We do appreciate you for pointing out the error in our publication and we are sorry for the confusion to the readers. Here is our response to your comment. After checking the original data and contacting with the first author Hongwei Liu, we found this mistake happened in the process of figure layout. When we added a scale to the picture, we put the picture with scale and without scale in the same folder. Then, two pictures were unintentionally added in the process of layout, resulting in duplication. This error has been corrected in the revised Fig 8e attached below. As all the experiments have been repeated at least 3 times, so this correction does not affect the interpretations or conclusions of this work. We re preparing a correction request that will be submitted to the Molecular Cancer journal shortly. We apologize to our readers for any confusion that this unintentional error might have caused. Best regardsuff01 Jian Huang ![file] , 
 Dear Dr. Dendrodoa Grossularia Thank you for pointing out the error in our published paper entilted with u201cInvasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p G3BP2 SRC FAK axisu201d. After communicating with Molecular Cancer journal, the correction to the paper has been published on 10 August 2020. The link to the correction is as follows: https: molecular-cancer.biomedcentral.com articles 10.1186 s12943-020-01241-2 Best regards! Jian Huang ,