Retracted: Cypermethrin exposure leads to regulation of proteins expression involved in neoplastic transformation in mouse skin
Authors: Jasmine George
Amit Kumar Srivastava
Yogeshwer Shukla
Richa Singh
Journal: PROTEOMICS (2011)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: Cypermethrin; carcinogenic effects; protein expression; neoplastic transformation; mouse skin
Affiliations: Proteomics Laboratory, CSIR-Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Lucknow, Uttar Pradesh, India., Proteomics Laboratory, CSIR‐Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Lucknow, Uttar Pradesh, India

Abstract:  Cypermethrin, a synthetic pyrethroid insecticide is shown to exert carcinogenic effects in rodents; however, its underlying mechanism remains elusive. Here, we showed the effect of cypermethrin on protein expression involved in neoplastic transformation in mouse skin. Comparative protein expression profiles between untreated control and cypermethrin-treated mouse skin were explored using 2-DE. A total of 27 spots that were statistically significant (p&lt;0.05) and differentially expressed in response to cypermethrin exposure were identified by MALDI-TOF/TOF and LC-MS/MS. Among them, six up-regulated proteins (carbonic anhydrase 3 (Ca 3), Hsp-27, S100A6, galectin-7, S100A9, S100A11) and one down-regulated protein (superoxide dismutase [Cu-Zn] (Sod 1)) are associated with cancer-related key processes. These selected dysregulated proteins were further validated in 2-DE gels of mouse skin treated with known tumorigens (benzo-[a]-pyrene, 12-O-tetradecanoyl-phorbol-13-acetate and mezerein), respectively. Comparative studies showed that Ca 3, S100A6, S100A9, S100A11 and Sod 1 are specific for stages of development and progression of tumors whereas Hsp-27 and galectin-7 are specific for tumor promotion stage by cypermethrin in mouse skin. Furthermore, these chosen proteins confirmed by Western blotting and immunofluorescence staining were consistent with changes in 2-DE check. This proteomic investigation for the first time provides key proteins that will contribute in understanding the mechanism behind cypermethrin-induced neoplastic transformation. 

Retracted: No

PubPeer comments:
 Figure 6: Compare column D Ca3 with column C HSP27. Same tissue section having overlapping segment. The difference is that of exposure. Next, compare column 3 Sod1 with S100A1. Exactly the same field with different exposure. Next, compare column 1 Ca3 with column D Sod1. Same field with different exposure. ![file] , 
 #1 You missed a pair. ![file] , 
 Figure 5: the bottom beta-actin panel appears to show two bands that look very similar. ![file] , 
 There is another pair in columns B and E of Figure 6: ![file] , 
 Figure 1(A) shows 2D protein expression for an **untreated control**. ![file] In Fig 1(A) of a concurrent publication, the same plot illustrates protein expression in a **vehicle control**, i.e. a mouse treated with acetone.  Neoplastic alterations induced in mammalian skin following mancozeb exposure using in vivo and in vitro models [(Tyagi et al 2011)] . ![file] It is a small inconsistency and I hope the authors can resolve it. , 
 At right:  Figure 2. Scatter plot presentation of protein expression comparisons between cypermethrin and ... mezerein Please join with in marvelling at the number of data points also present in the scatter-plot of protein expressions at left: Fig 1 from  Early changes in proteome levels upon acute deltamethrin exposure in mammalian skin system associated with its neoplastic transformation potential [(George Sukla 2013)] . Even though the latter compares **delta**methrin with B[a]P. ![file] ,