MiR-340 affects gastric cancer cell proliferation, cycle, and apoptosis through regulating SOCS3/JAK-STAT signaling pathway
Authors: Hongyan Cao
Chunyan Guo
Weibing Li
Jianfen Yuan
Chunhong Xiao
Hong Hong
Haizhong Yu
Journal: Immunopharmacology and Immunotoxicology (2018)
Discipline: IMMUNOLOGY
Keywords: JAK-STAT3; SOCS3; apoptosis; cell cycle; gastric cancer
Affiliations: a Department of Clinical Laboratory , Nantong Tumor Hospital , Nantong , Jiangsu , China., b Department of Clinical Laboratory , Nantong Traditional Chinese Medicine Hospital , Nantong , Jiangsu , China.

Abstract:  Objective: Janus kinase (JAK)-signal transducer and activator of transcription (STAT) signaling pathway is closely related to tumorigenesis. Suppressors of cytokine signaling 3 (SOCS3) is a negative regulator of JAK-STAT signaling pathway. MiR-340 expression is significantly upregulated in gastric cancer (GC) tissue. This study investigated the role of miR-340 in regulating SOCS3 expression and affecting GC cell proliferation, cycle, and apoptosis. Patients and methods: Dual luciferase assay was used to verify the targeted relationship between miR-340 and SOCS3. GC tissue was collected from patients. Normal gastric mucosal tissue was selected as control. MiR-340, SOCS3, p-JAK, p-STAT3, and Survivin protein expressions were compared with GES-1 and MKN-28 cells. MKN-28 cells were cultured in vitro and divided into four groups, including miR-NC, anti-miR-340, pSicoR-Blank, and pSicoR-SOCS3 groups. Cell proliferation, cycle, and apoptosis were detected by flow cytometry. Results: Bioinformatics analysis revealed the targeted relationship between miR-340 and the 3'-UTR of SOCS3 mRNA. Dual luciferase assay demonstrated that miR-340 regulated SOCS3 expression. MiR-340 level was significantly elevated, while SOCS3 level was obviously declined in GC tissue compared with normal mucosal tissue. MiR-340, p-JAK, p-STAT3, and Survivin expressions were upregulated, whereas SOCS3 expression was reduced in MKN-28 cells compared with that in GES-1 cells. Anti-miR-340 or pSicoR-SOCS3 transfection markedly increased SOCS3 expression, reduced p-JAK, p-STAT3, and Survivin levels, attenuated cell proliferation, arrested cell cycle, and enhanced cell apoptosis in MKN-28 cells. Conclusions: Downregulation of miR-340 inhibited GC cell proliferation, arrested cell cycle, and facilitated apoptosis through upregulating SOCS3 expression to suppress JAK-STAT3 signaling pathway. 

Retracted: No

PubPeer comments:
 ![file] These are extremely stylised Western Blots, solid blobs of black on a smooth grey background as if painted by Miro. Compare with (for instance) figures in the now-retracted paper [Wu et al (2017)] . ![] , 
 Expression of Concern issued [12 October 2020] :  After publication of this article, questions about the scientific integrity of the article content were brought to the Publisher and Editoru2019s attention. We have reached out to the authors requesting that they supply information that would confirm the articleu2019s integrity, but the authors have not responded to our queries within the requested timeframe. Therefore, as we continue to work through the issues raised, we advise readers to interpret the information presented in the article with due caution. , 
 Dear editor, In view of the transformation of the laboratory, the original of Western blot images is missing. In order to prevent misunderstanding, the manuscript is withdrawn first, and the new Western blot images will be sent after re testing. We apologize for any inconvenience to you. Yours sincerely, , 
 #3 Personally, I am unconvinced that western blots of these experiments ever existed as these images are mere parodies of what western blot scans look like. The lack of any noise in the images suggest an artificial source. To help reassure readers, could the corresponding author confirm that these experiments gels were generated and scanned at the Nantong Tumor Hospital, or the Traditional Chinese Medicine Hospital, by the authors of this paper? I wonder if facilities have the necessary equipment, but perhaps my skepticism is misplaced. However given the other papers posted here on PubPeer, I think my skepticism is warranted: https: pubpeer.com publications 8D0B222EF943150F5F4E905B3B9BFA *** ![file] , 
 Not waiting for a final verdict from the journal, the authors hospital has reprimanded and disciplined them: http: www.ntzyy.com news 1125.html Thanks Google Translate: ----------------------- **Announcement of Nantong Hospital of Traditional Chinese Medicine on the Investigation and Handling of Yu Haizhong s Academic Misconduct** In order to strengthen the integrity of scientific research, severely punish violations, and create a good environment for scientific and technological innovation, we hereby report the relevant paper issues recently investigated and dealt with by our unit as follows: As the corresponding author, Xiao Chunhong, Nantong Hospital of Traditional Chinese Medicine, Yu Haizhong, the first author of Nantong Tumor, published the paper  MiR-340 affects gastric cancer cell proliferation, cycle, and apoptosis through regulating SOCS3 JAK-STAT signaling pathway in Immunopharmacology and Immunotoxicology. After investigation, the data was falsified, which is academic misconduct. The Nantong Hospital of Traditional Chinese Medicine decided to give Comrade Yu Haizhong the following treatment after research: 1. Announce the criticism; 2. Withdraw the above-mentioned papers published; 3. Cancel the qualification for applying for financially funded science and technology plans (special projects, funds, etc.) for 8 years (involving other paper fraud , Combined punishment), to recover the one-time bonus for the paper; 4. Rectify and strengthen the management of the academic team, and cancel the qualifications of the academic committee. Nantong Tumor Hospital decided to give Comrade Xiao Chunhong the following treatment after research: 1. Announce criticism; 2. Withdraw the above-mentioned papers published; 3. Suspend financial support for science and technology projects for 8 years (involving other paper frauds, combined punishment), and recover the paper once Sex bonus. Nantong Traditional Chinese Medicine Hospital September 08, 2021 , 
 http: med.china.com.cn content pid 291148 tid 1013 iswap 1 The paper  MiR-340 affects gastric cancer cell proliferation, cycle, and apoptosis through regulating SOCS3 JAK is published in the article  MiR-340 affects gastric cancer cell proliferation, cycle, and apoptosis through regulating SOCS3 JAK -STAT signaling pathway , after investigation, is the academic misconduct of tampering with data. 1. The Nantong Hospital of Traditional Chinese Medicine decided to give Comrade Yu Haizhong the following treatment after research: Announce criticism, withdraw the above-mentioned papers published, and cancel the qualifications for applying for financially funded science and technology plans (special projects, funds, etc.) for 8 years (involving other paper frauds, combined punishment) , Recover the one-time bonus for thesis, rectify and strengthen the management of the academic team, and cancel the qualification of the academic committee; 2. Nantong Tumor Hospital decided to give Comrade Xiao Chunhong the following treatment after research: circulate criticism, withdraw the above-mentioned published papers, suspend financial funding of scientific and technological projects for 8 years (involving other paper frauds, combined punishment), and recover the one-time bonus for papers. (Notification link: http: www.ntzyy.com news 1125.html) 3. As the first author and corresponding author, Yi Tongbo, Taizhou Peopleu2019s Hospital, Jiangsu Province, published the paper  Activation of lncRNA lnc SLC4A1- 1 induced by H3K27 acetylation promotes the development of Nanomedicine and Biotechnology (2019, 47: 3765-3773) of breast cancer viaactivating CXCL8 and NF kB pathway , after investigation, there are academic misconducts such as third-party proxy investment, trading data, improper attribution, etc. Taizhou Peopleu2019s Hospital dealt with the relevant responsible person Yi Tongbo: He was advised to contact the magazine to withdraw the manuscript of the article involved, to recover the reimbursed page fee and the award for the paper, and he was not allowed to apply for position or title promotion within 5 years, and was not allowed to participate in scientific research project approval and achievement awards within 5 years. (Notification link: http: www.jstzhospital.com contents 13 2919.html) ,