Differential gene expression in proximal and distal nerve segments of rats with sciatic nerve injury during Wallerian degeneration
Authors: Nan Jiang
Dexin Yin
Qin Zhao
Yi Sun
Shusen Cui
Huaiqin Li
Dengbing Yao
Journal: Neural Regeneration Research (2014)
Discipline: NEUROSCIENCE & BEHAVIOR
Keywords: NSFC grant; Wallerian degeneration; biological process; expression profiling; microarray
Affiliations: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China ; China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China., Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China., China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China., Key Laboratory of People's Liberation Army, Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China.

Abstract:  Wallerian degeneration is a subject of major interest in neuroscience. A large number of genes are differentially regulated during the distinct stages of Wallerian degeneration: transcription factor activation, immune response, myelin cell differentiation and dedifferentiation. Although gene expression responses in the distal segment of the sciatic nerve after peripheral nerve injury are known, differences in gene expression between the proximal and distal segments remain unclear. In the present study in rats, we used microarrays to analyze changes in gene expression, biological processes and signaling pathways in the proximal and distal segments of sciatic nerves undergoing Wallerian degeneration. More than 6,000 genes were differentially expressed and 20 types of expression tendencies were identified, mainly between proximal and distal segments at 7-14 days after injury. The differentially expressed genes were those involved in cell differentiation, cytokinesis, neuron differentiation, nerve development and axon regeneration. Furthermore, 11 biological processes were represented, related to responses to stimuli, cell apoptosis, inflammatory response, immune response, signal transduction, protein kinase activity, and cell proliferation. Using real-time quantitative PCR, western blot analysis and immunohistochemistry, microarray data were verified for four genes: aquaporin-4, interleukin 1 receptor-like 1, matrix metalloproteinase-12 and periaxin. Our study identifies differential gene expression in the proximal and distal segments of a nerve during Wallerian degeneration, analyzes dynamic biological changes of these genes, and provides a useful platform for the detailed study of nerve injury and repair during Wallerian degeneration. 

Retracted: No

PubPeer comments:
 These two group were same image! ![file] , 
 The authors do not match the orginal paper! ![file] , 
 #1 We thank you very much for pointing this out, as we hadnu2019t noticed this error till you mentioned it. In fact, the images of MMP12 staining at 14d, 21d, and 28d appear to be an exact repeat of those at 0d, 4d, and 7d. We checked the original data as well as the Ph.D. thesis and found that was a mistake during the Figure arrangement. We are really sorry about this and have corrected them. The corrigendum is available online https: pubmed.ncbi.nlm.nih.gov 33642367 ![file] , 
 #2 Thank you very much for your comments. This manuscript underwent quite a long period of revise and some necessary experiments were additionally added according to the revieweru2019s suggestions. So, the author s order changed in the revised manuscript according to the contribution. ,