MicroRNA-494 inhibits proliferation and metastasis of osteosarcoma through repressing insulin receptor substrate-1
Authors: Yansong Wang
Gang Lv
Yang Yu
Peng Yan
Kai Wu
Xiaodong Zhi
Deshui Yu
Journal: American journal of translational research (2016)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: metastasis; miR-494; osteosarcoma; proliferation.
Affiliations: Department of Spine Surgery, The First Affiliate Hospital of Liaoning Medical University Jinzhou 121000, China., Department of Trauma, The First Affiliate Hospital of Liaoning Medical University Jinzhou 121000, China., Department of Joint, The First Affiliate Hospital of Liaoning Medical University Jinzhou 121000, China.

Abstract:  Despite microRNA-494 (miR-494) has a well-established role in many types of cancer; the biological function and potential mechanism of miR-494 in human osteosarcoma (OS) has not been elucidated. The aim of this study was therefore to investigate the role and underlying mechanism of miR-494 expression in osteosarcoma. Here, we found that miR-494 was significantly decreased in OS tissues and cell lines compared to the adjacent noncancerous bone tissues (P&lt;0.01) and human normal osteoblast cells (NHOst) (P&lt;0.05), respectively. Functional assays demonstrated that ectopic overexpression of miR-494 could significantly inhibit cell proliferation, colony formation, migration and invasion in vitro, as well as suppress tumor growth in nude mice model. Further integrative and functional studies suggested insulin receptor substrate 1 (IRS1) as a target gene of miR-494 in OS cells. IRS1 expression was upregulated, and inversely correlated with miR-494 expression in clinical OS tissues (r=-0.589, P=0.001). Moreover, downregulation of IRS1 had similar the inhibition effect on cell proliferation, colony formation, migration and invasion of miR-494 overexpression. Overexpresion of miR-494 obviously decreased AKT signal pathway activation. These findings suggested that miR-494 functioned as a tumor suppressor in OS, at least in part, by targeting IRS1. 

Retracted: No

PubPeer comments:
 Figure 4B: the images of clone 1 and clone 2 appear to be the same image, e.g. if the clone 2 image is rotated 90 degrees clockwise and rescaled, they appear to be identical. ![file] original figure below: ![file] , 
 observation from Actinopolyspora Biskrensis Fig 4B. the  wild image (shown in comment #1) appears to be a duplicate of one of the images shown in Figure 3C and 3E in: Yi Yang et al. Autophagy regulates the stemness of cervical cancer stem cells Biologics: Targets and Therapy 2017:11 71u201379 10.2147 BTT.S134920 Fig 3C and E from citation immediately above compared to Fig 4 from Fu et al. Bioscience Reports (2017) 37 BSR20160523; 10.1042 BSR20160523 ![file] , 
 not sure who is copying from whom but there s another duplication between the 2 papers cited in comment #2 ![file] , 
 and yet more inter-paper duplication and manipulation ![file] , 
 borrowing from Smut Clyde and continuing from comment #1 ![file] , 
 yet another duplication between the 2 papers cited Fu and Yang papers ![file] , 
 and just because there s never too much duplication and manipulation.... Fig 5 from Yang and Fig 4 from Fu with duplicated areas in red box. ![file] , 
 The Editorial Office has been made aware of potential issues surrounding the scientific validity of this paper, hence has issued an expression of concern to notify readers whilst the Editorial Office investigates. , 
 #9 While it does appear that this paper was submitted before the Biologics paper, how did the other paper come to be submitted while this paper was under review and who was responsible for the image manipulation? It is especially puzzling and concerning given the apparent overlap in the authors. This paper: Rui Fu 1, **Yan Ding** 2, Jie Luo 1, **Li Yu** 2, Cheng Lin Li 2, **Dong Sheng Li** 2 and Shi Wen Guo 1 1 Department of neurosurgery, affiliated Taihe hospital of Xiu2019an Jiaotong University Health Science Center, Shiyan, Hubei, China; 2 Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China Biologics. 2017; 11: 71u201379: Yi Yang, 1,2 **Li Yu**, 1 Jin Li, 1 Ya Hong Yuan, 1 Xiao Li Wang, 1 Shi Rong Yan, 1 **Dong Sheng Li**, 1 and **Yan Ding** 1 1 Hubei Key Laboratory of Embryonic Stem Cell Research 2 Reproductive Center, Taihe Hospital, Hubei University of Medicine, Shiyan, Peopleu2019s Republic of China , 
 Iu2019m not sure this was covered in the thread already, but Figure 2F appears to have two images which overlap slightly. *** ![file] , 
 Retracted [14 May 2021] . ---------------------------------------- This Retraction follows an Expression of Concern relating to this article previously published by Portland Press. This article is being retracted from Bioscience Reports at the request of the Editor-in-Chief and the Editorial Board following receipt of a notification from a reader, alerting the Editorial Board to duplicated images in Figures 2F, 2I and 4B which are also published in Biologics: Targets and Therapy (10.2147 BTT.S134920). Images are also duplicated within the Bioscience Reports article, in Figures 2F and 4B. The authors have been contacted in regards to the retraction, and disagree with the Journal s decision to retract the article; however, the Editorial Board stand by the decision to retract given the quantity and extent of the concerns with the figures. ,