<p>LncRNA PTTG3P Sponge Absorbs microRNA-155-5P to Promote Metastasis of Colorectal Cancer</p>
Authors: Xinxin Zhang
Lei Dou
Ning Liu
Journal: OncoTargets and Therapy (2020)
Discipline: CLINICAL MEDICINE
Keywords: CRC; LncRNA PTTG3P; metastasis; microRNA-155-5P.
Affiliations: Department of Pancreatic and Biliary Surgery, The First Affiliated Hospital of China Medical University, Shenyang, People's Republic of China., Department of Pathology, China Medical University, Shenyang, People's Republic of China.

Abstract:  Objective: To study the role of LncRNA PTTG3P in colorectal cancer (CRC) and the underlying mechanism. Patients and methods: The expression level of LncRNA PTTG3P was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) in CRC cell lines and tumor tissues from 43 CRC patients, and the correlations between LncRNA PTTG3P expression and the clinicopathological indicators and prognosis of CRC patients were analyzed. Then, a PTTG3P knockdown model in CRC cell lines HCT-8 and HCT-116 was constructed. Finally, the relationship between LncRNA PTTG3P and microRNA-155-5p was explored through luciferase reporter experiments and recovery experiments. Results: qRT-PCR results showed that LncRNA PTTG3P was markedly up-regulated in CRC tumor tissues than that in adjacent tissues. Meanwhile, patients with high LncRNA PTTG3P expression had higher rates of lymph node metastasis and distant metastasis. In addition, cell functional experiments suggested that knocking down PTTG3P markedly reduced the migration abilities of CRC cells. Subsequently, bioinformatics analysis and luciferase reporter gene experiments suggested that LncRNA PTTG3P could directly bind to microRNA-155-5P. Analysis of CRC tissue samples showed that microRNA-155-5P expression was markedly reduced in CRC and was negatively correlated with LncRNA PTTG3P. Finally, the recovery experiments also suggested that there was a mutual regulation between LncRNA PTTG3P and microRNA-155-5P, and silencing microRNA-155-5P can reverse the inhibitory effect of knocking down PTTG3P on the malignant progression of CRC. Conclusion: In summary, LncRNA PTTG3P level was markedly increased in CRC, and was highly correlated to the incidence of lymph node metastasis and distant metastasis in CRC patients. In addition, LncRNA PTTG3P might promote the ability of CRC to invade and migrate by downregulating microRNA-155-5P. Therefore, dissecting the aberrant regulation of LncRNA PTTG3P/microRNA-155-5P may be valuable for early screening, guidance treatment, and recurrence detection of CRC. 

Retracted: No

PubPeer comments:
 Figs 1D, 3D.  Kaplan Meier survival curves was plotted to evaluate the relationship between LncRNA PTTG3P expression and the prognosis of patients ...  Kaplan Meier survival curves was plotted to evaluate the relationship between microRNA-155-5P expression and prognosis . ![file] This distinctive style of paired K-M plots, resembling combs or Lego walls with the precipitous drop in survival at 75 months, is one characteristic feature of papermill. Other products are tabulated in a Google sheet: https: docs.google.com spreadsheets d 1_762PbYYTCJhek5PdBuiDuN1q48UDgMa-rT6gi_jA8c edit?usp=sharing Here I have superimposed the two pairs, to emphasise that for most of the post-surgery time-course, overall survival is better when patients are dichotomised by microRNA-155-5P expression (in red) ![file] , 
 Fig 2C,  Cell scratch test was performed to detect the crawling ability of CRC cells HCT-8 and HCT-116 after transfection of PTTG3P knockdown vector . Two cell-lines seem to be interchangeable. ![file] ,