Adipose stem cells’ antagonism in glycosylation of D-galactose-induced skin aging of nude mice and its skin recovery function
Authors: Shuyue Wei
Xinxin Xue
Yuntian You
Haiying Wang
Qiang Ma
Journal: International Journal of Immunopathology and Pharmacology (2016)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: antagonism of adipose stem cells; free radicals; glycosylation; systemic aging.
Affiliations: Department of Dermotology, Dongying People's Hospital of Shandong Provincial Hospital Group, Dongying, Shandong, PR China., Binzhou Medical University, Yantai, Shandong, PR China., Department of Dermotology, Dongying People's Hospital of Shandong Provincial Hospital Group, Dongying, Shandong, PR China .

Abstract:  This study aims to discuss adipose stem cells' (ASCs) antagonism in glycosylation of D-galactose-induced skin aging of nude mice and its skin recovery function; the study also aims to explore a new mechanism of anti-aging to provide clinical anti-aging therapy with new thoughts and methods. We selected 40 healthy specific pathogen-free (SPF) nude mice and divided them randomly into four groups which were: blank control group; D-galactose + phosphate buffer saline (PBS) group; D-galactose + ASCs treatment group; and D-galactose + aminoguanidine (AG) group. Results showed that the superoxide dismutase (SOD) level of mice in the D-galactose-induced model group (87.15 ± 4.95 U/g) decreased significantly compared with that of control group (146.21 ± 4.76 U/g), while malonaldehyde (MDA) level of mice in D-galactose induced model group (11.12 ± 2.08 nmol/mg) increased significantly compared with that of control group (5.46 ± 2.05 nmol/mg) (P &lt;0.05); thus D-galactose induced sub-acutely aging mice models were duplicated successfully. Results also indicated that transplantation of ASCs could reverse expression of aging-related biomarkers such as MDA, SOD, and advanced glycosylation end products (AGEs); hematoxylin and eosin (HE) staining showed that thickness of the dermis layer as well as the collagen content of mice in the D-galactose-induced model group increased significantly after ASC transplantation compared with that of control group. In addition, immunohistochemical assay showed that expression quantity of CD31 and vascular endothelial growth factor (VEGF) of mice in the D-galactose-induced model group increased significantly after ASC transplantation compared with that of control group. In conclusion, ASCs can trace cell distribution successfully through bioluminescence, and they survive for a short time in the skin after transplantation, which provides a basis for the application of ASC transplantation in clinical practices. Moreover, ASCs can control glycosylation level of D-galactose-induced skin aging of nude mice, reverse expression of aging-related biomarkers as well as restrain formation of advanced glycation end products, which are similar to the effects of AG inhibitors of advanced glycation end products. Thus, ASCs can prevent glycosylation-induced skin aging as well as recover functions of skin. 

Retracted: No

PubPeer comments:
 Figure 5: each panels appears to show some repetitive features. ![file] The legend reads:  HE staining comparison among four groups: (a) Control group; (b) D-gal group; (c) D-gal+AG group; (d) D-gal+ASCs group. Proportion was 100 um (u00d7200); 40 6-week- old SPF nude mice, 10 in each group. , 
 Figure 7A might also deserve some scrutiny, at least according to Forensically: ![file] ,