Expression of mTOR/70S6K signaling pathway in pathological scar fibroblasts and the effects of resveratrol intervention
Authors: Xiao-Xiang Zhai
Zhi-Ming Tang
Ji-Cun Ding
Journal: Molecular Medicine Reports (2017)
Discipline: CLINICAL MEDICINE
Keywords: mTOR; 70S6K; pathological scar; resveratrol; fibroblasts
Affiliations: Department of Dermatology, Xuzhou Hospital of Traditional Chinese Medicine, Xuzhou, Jiangsu 221003, P.R. China., Department of Burns and Plastic Surgery, The Affiliated Xuzhou Center Hospital of Nanjing University of Chinese Medicine, Xuzhou, Jiangsu 221009, P.R. China.

Abstract:  The aim of the study was to examine the expression of mammalian target of rapamycin (mTOR)/70S6K signaling pathway in pathological scar fibroblasts and the effects of resveratrol (Res) intervention. The mTOR and 70S6K in pathological scar and normal skin fibroblasts were detected by immunofluorescence following treatment with different concentrations of Res. RT-PCR and western blot analysis were used to detect the expression of mTOR and 70S6K mRNA and protein, respectively. Immunofluorescence showed that the expression of 70S6K and mTOR was significantly enhanced in pathological scar fibroblasts, and mainly expressed in the nucleus, but not in normal skin fibroblasts. RT-PCR and western blot analysis showed that after different concentrations of Res treatments, the mTOR and 70S6K mRNA and protein expression significantly (P&lt;0.05) decreased in a dose‑dependent manner. In conclusion, the expression of mTOR/70S6K signaling pathway in pathological scar fibroblasts was significantly enhanced. Res can downregulate the expression of mTOR and 70S6K to achieve the inhibition of pathological scar fibroblast proliferation. 

Retracted: No

PubPeer comments:
 ![file] Figure 4 : The normal tissue section was reused from another study by the same authors (Singh et al., 2003, Mol. Microbiol, among others) where the tissue is labled as 6w pi (vs 8w pi in this paper) , 
 *In the manuscript by Rao et al., 2005, Scandinavian Journal of Immunology, the focus was to study the histopathological tissue damage in the lungs of the animals from BCG or rBCG or sham immunized groups after 8 weeks post-M. tuberculosis infection.* *In the study by Singh et al., Molecular microbiology, 2003, the focus was to compare the extent of tissue damage and cellular infiltration in granulomas in the lungs of guinea pigs infected with either wild type or mptpB mutant or mptpB complemented M. tuberculosis strains at both 3 weeks and 6 weeks post-infection.* *The normal lung tissue section shown in Singh et al., Molecular Microbiology 2003 (Fig. 6 and Fig. 7) and Rao et al., 2005 (Fig. 4) refers to sections from uninfected unvaccinated animal and was used for normal tissue histology. The animal experiments shown in these studies were performed at Tuberculosis Research Centre, Chennai, India and multiple experiments were being conducted simultaneously. As per CPCSEA guidelines, users have the responsibility to obtain the maximum information from minimum number of animals used for experiments. Therefore, the same lung tissue from uninfected and unvaccinated animal was included in these studies just as a reference for normal tissue histology. The inclusion exclusion of u201cNormal lungu201d panel in these studies does not have any bearing on the scientific conclusions of these manuscripts in any manner.* , 
 The correction note for Rao et al., 2005 is available at https: doi.org 10.1111 sji.12991 , 
 #3 Will you be able to post the text here than the link? i don t want pay USD14 for 48 h access to read the correction note. I think most journals should start making corrections open access than asking for access fee. ,