REMOVED: miR-4295 promotes cell proliferation and invasion in anaplastic thyroid carcinoma via CDKN1A
Authors: Sidong Wei
Qingxia Fan
Liuxing Wang
Yiwei Geng
Peng Lu
Mingchen Shao
Wang Ma
Ying Xi
Journal: Biochemical and Biophysical Research Communications (2015)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: Anaplastic thyroid carcinoma; CDKN1A; miR-4295.
Affiliations: Oncology Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; Laboratory of Tumor Biology, Zhengzhou University, Zhengzhou, China., Gastrointestinal Surgery Department, People's Hospital of Zhengzhou, Zhengzhou, China., Liver Transplantation Hepatobiliary Surgery Department, People's Hospital of Zhengzhou, Zhengzhou, China.

Abstract:  MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. However, the role of microRNAs in anaplastic thyroid carcinoma (ATC), has remained elusive. Here, we identified that miR-4295 promotes ATC cell proliferation by negatively regulates its target gene CDKN1A. In ATC cell lines, CCK-8 proliferation assay indicated that the cell proliferation was promoted by miR-4295, while miR-4295 inhibitor significantly inhibited the cell proliferation. Transwell assay showed that miR-4295 mimics significantly promoted the migration and invasion of ATC cells, whereas miR-4295 inhibitors significantly reduced cell migration and invasion. luciferase assays confirmed that miR-4295 directly bound to the 3'untranslated region of CDKN1A, and western blotting showed that miR-4295 suppressed the expression of CDKN1A at the protein levels. This study indicated that miR-4295 negatively regulates CDKN1A and promotes proliferation and invasion of ATC cell lines. Thus, miR-4295 may represent a potential therapeutic target for ATC intervention. 

Retracted: Yes

PubPeer comments:
 This article has been retracted: please see Elsevier Policy on Article Withdrawal (http: www.elsevier.com locate withdrawalpolicy). This article has been retracted at the request of the Editor-in-Chief. The Western blots from Figure 1A are similar to Western blots from Figures 3A and 3D of the paper published by Yongfang Wang, Lianhong Xu and Lixin Jiang in Biochemical and Biophysical Research Communications (BBRC) 458 (2015) 714-719 http: dx.doi.org 10.1016 j.bbrc.2015.02.033 and Figures 3A and 3D of the paper published by Hong Zhu, Mei-hua Miao, Xue-qiang Ji, Jun Xue and Xue-jun Shao in BBRC 459 (2015) 340-345 http: dx.doi.org 10.1016 j.bbrc.2015.02.116. In addition, a significant amount of textual overlap has been noticed between this article and the articles published by BBRC 458 (2015) 714-719 and BBRC 459 (2015) 340-345. One of the conditions of submission of a paper for publication is that authors declare explicitly that their work is original and has not appeared in a publication elsewhere. Re-use of any data should be appropriately cited. As such this article represents a severe abuse of the scientific publishing system. The scientific community takes a very strong view on this matter and apologies are offered to readers of the journal that this was not detected during the submission process. ,