miR-196B-5P and miR-200B-3P Are Differentially Expressed in Medulloblastomas of Adults and Children
Authors: Dario de Biase
Francesca Romana Buttarelli
Annalisa Pession
Alba Ariela Brandes
Alessia Ciarrocchi
Gianluca Marucci
Michela Visani
Enrico Franceschi
Giorgia Acquaviva
Kerry Jane Rhoden
Giovanni Tallini
Felice Giangaspero
Journal: Diagnostics (2020)
Discipline: CLINICAL MEDICINE
Keywords: adult medulloblastoma; childhood medulloblastoma; microRNA; microRNA profile.
Affiliations: Department of Specialized, Diagnostic and Experimental Medicine, Anatomic Pathology-Molecular Diagnostic Unit AUSL-IRCCS of Bologna, University of Bologna School of Medicine, 40138 Bologna, Italy., Anatomic Pathology Unit, Ospedale Bellaria AUSL-IRCCS of Bologna, 40139 Bologna, Italy., Department of Pharmacy and Biotechnology (FaBiT), Molecular Diagnostic Unit AUSL of Bologna, University of Bologna, 40138 Bologna, Italy., Department of Radiological, Oncological and Anatomo-Pathological Sciences, Sapienza University School of Medicine, 00161 Rome, Italy., IRCCS Neuromed, 86077 Pozzilli (Isernia), Italy., Department of Human Neurosciences, Sapienza University School of Medicine, 00161 Rome, Italy., Department of Medical Oncology, Bellaria-Maggiore Hospitals AUSL-IRCCS of Bologna, 40139 Bologna, Italy., Laboratory of Translational Research, Arcispedale Santa Maria Nuova AUSL-IRCCS of Reggio Emilia, 42122 Reggio Emilia, Italy., Department of Medical and Surgical Sciences, Medical Genetics Unit, University of Bologna School of Medicine, 40138 Bologna, Italy.

Abstract:  Medulloblastoma is a highly aggressive brain tumor that typically affects children, while in adults it represents ~1% of all brain tumors. Little is known about microRNA expression profile of the rare adult medulloblastoma. The main aim of this study was to identify peculiar differences in microRNA expression between childhood and adult medulloblastoma. Medulloblastomas were profiled for microRNA expression using the Exiqon Human miRNome panel (I + II) analyzing 752 microRNAs in a training set of six adult and six childhood cases. Then, the most differentially expressed microRNAs were validated in a total of 21 adult and 19 childhood cases. Eight microRNAs (miR-196b-5p, miR-183-5p, miR-200b-3p, miR-196a-5p, miR-193a-3p, miR-29c-3p, miR-33b-5p, and miR-200a-3p) were differentially expressed in medulloblastoma of adults and children. Analysis of the validation set confirmed that miR-196b-5p and miR-200b-3p were significantly overexpressed in medulloblastoma of adults as compared with those of children. We followed an in silico approach to investigate direct targets and the pathways involved for the two microRNAs (miR-196b and miR-200b) differently expressed between adult and childhood medulloblastoma. Adult and childhood medulloblastoma have different miRNA expression profiles. In particular, the differential dysregulation of miR-196b-5p and miR-200b-3p characterizes the miRNA profile of adult medulloblastoma and suggests potential targets for novel diagnostic, prognostic, or therapeutic strategies. 

Retracted: No

PubPeer comments:
 Another paper from [a group with common author] . Figure 1 appears to have two images which overlap, but are described differently. *** ![file] , 
 Dear, in the original article, there was a mistake in Figure 1 as published. The IHC of u201cWNT subgroup+GAB1 was incorrectly the same IHC as in u201cnon-SHH WNT sub-group+YAP1 . The authors apologize for any inconvenience caused and state that the scientific conclusions are unaffected. The original article will be updated. Sincerly Dr. Visani , 
 Published September 7, 2021  The authors wish to make the following corrections to this paper [1]: In the original article, there was a mistake in Figure 1 as published. The IHC of u201cWNT subgroup + GAB1u201d was incorrectly the same IHC as in u201cnon-SHH WNT subgroup+YAP1.u201d We probably made a mistake while assembling the picture. The corrected Figure 1 appears below. The authors apologize for any inconvenience caused and state that the scientific conclusions are unaffected. The original article has been updated. ![file] ,