LncRNA GIHCG regulates microRNA-1281 and promotes malignant progression of breast cancer
Authors: P Yang
L-P Ren
D Xu
L-Y Fan
K-Y Shi
F Wang
G-L Shao
L Zhang
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: lncRNA; GIHCG; breast cancer; prognosis; clinical characteristics
Affiliations: Institute of Cancer and Basic Medicine (ICBM), Chinese Academy of Sciences, Zhejiang, China. .

Abstract:  Objective: This study aimed to investigate the expression characteristics of long non-coding RNA (lncRNA) GIHCG in breast cancer (BCa), and further investigate its role in BCa and its relationship with clinical characteristics and prognosis. Patients and methods: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was performed to examine GIHCG expression in 53 pairs of BCa tumor tissues and adjacent tissues. The interaction between the level of GIHCG and the clinical indicators of BCa and the prognosis of patients was then analyzed. Lentivirus was transfected into BCa cell lines to construct the GIHCG knockdown model. The cell counting kit-8 (CCK-8), cell cloning, and 5-Ethynyl-2'-deoxyuridine (EdU) assays were performed to analyze the influence of GIHCG on the biological function of BCa cells, as well as to explore whether it could play a role via modulating microRNA-1281. Results: QRT-PCR results showed that the GIHCG level was remarkably higher in the BCa tumor tissue than in adjacent ones. Compared with patients with low expression of GIHCG, patients with high expression of GIHCG had higher pathological grades and a lower overall survival. Besides, the proliferation ability of BCa cells in GIHCG knockdown group was significantly decreased compared with NC group. QRT-PCR results indicated that silencing GIHCG increased the expression of miR-1281, thereby promoting the malignant progression of BCa. Also, the silence of miR-1281 reversed the effect of GIHCG on the proliferative capacity of BCa, thus increasing the cell anti-apoptotic ability. Conclusions: GIHCG levels were remarkably increased in both BCa tissues and cells, which was related to the pathological stage and poor prognosis of BCa patients. Besides, GIHCG might promote the malignant progression of BCa by inhibiting microRNA-1281. 

Retracted: No

PubPeer comments:
 Supplemental Fig. S2F u2013 panels seems to be duplicated ![file] , 
 Thanks for your kind remind! This is Yuting MA, the first author of this article. Since these experiments were performed more then 10 years ago, I did spend several hours to check our previous records. I managed to find one of our original FACS data (.fcs file) and reanalyzed it with Flowjo software. Please find the uploaded .jpg file, which clearly shows the gating strategy and cytokine secretion by the bulk gdT cells (CD3+TCRdelta+) and Vgamma4+ gdT cells. I can share the original .fcs file, if needed. However, Pubpeer doesn t accept this file format. Since I have changed my working positions and computers multiple times, I couldn t find our previous exported files in my computer. But this file strongly support the conclusion that gdT cells and Vgamma4+ gdT cells in the lymph nodes of naive mice can produce high levels of IL-17, but they don t produce IFN-g upon PMA+ionomycin stimulation. ![file] In our previous supplemental Figure. 2F, the IFN-g vs TCR delta plot does look similar with the IFN-g vs Vgamma 4 plot. It might be a careless copy-and-paste mistake. We will contact the publisher and correct this point. Your kind remind is appreciated! Best regards Yuting MA ,