Circular RNA hsa_circ_0072309 inhibits non-small cell lung cancer progression by sponging miR-580-3p
Authors: Yanming Huang
Wenguang Pang
Jingzhuo Pang
Xin Zhang
Zheng Wang
Chengjie Cai
Xiufang Huang
Fengliu Huang
Min Ye
Journal: Bioscience Reports (2020)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: hsa_circ_0072309; miR-580-3p; non-small cell lung cancer.
Affiliations: Department of Thoracic Surgery, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China, Department of Thoracic Surgery, Jiangmen Central Hospital, Jiangmen 529030, China, Department of Respiration Medicine, Jiangmen Central Hospital, Jiangmen 529030, China, Clinical Experimental Center (Clinical Biobanks), Jiangmen Central Hospital, Jiangmen 529030, China, Department of Pathology, Jiangmen Central Hospital, Jiangmen 529030, China, Department of Thoracic Surgery, The 2nd Clinical Medical College of Jinan University, Shenzhen 518020, China

Abstract:  Objective: Non-small cell lung cancer (NSCLC) continues to top the list of cancer mortalities worldwide. Early diagnosis and therapeutic interventions targeting NSCLC is becoming the world's significant challenge. Circular RNAs (circRNAs) are emerging as a group of potential cancer biomarkers. Materials and methods: Quantitative real-time PCR (qRT-PCR) was employed to examine the expression of circ_0072309 in NSCLC tissues and cell lines. Cell counting kit 8 (CCK-8), wound healing and Transwell assays were used to analyze cell proliferation, migration and invasion in A549 and H1299 cells. The relationship between circ_0072309 and miR-580-3 was analyzed by Luciferase reporter and RNA pull down assays. Results: We screened circ_0072309 from Gene Expression Omnibus and found that circ_0072309 was lowly expressed in NSCLC tissues and cell lines. The transfection of circ_0072309-overexpressing vector significantly suppressed the cell proliferation, migration and invasion in A549 and H1299 cells. We predicted that miR-580-3p is a target of circ_0072309 by using publicly available bioinformatic algorithms Circinteractome tool and confirmed that circ_0072309 directly bound to miR-580-3p. Furthermore, the addition of miR-580-3p mitigated the blockage of cell proliferation, migration and invasion induced by circ_0072309. Conclusions: These data showed that circ_0072309 inhibits the progression of NSCLC progression via blocking the expression of miR-580-3p. These findings revealed the anti-tumor role of circ_0072309 during the development of NSCLC and provided a novel diagnostic biomarker and potential therapy for NSCLC. 

Retracted: No

PubPeer comments:
 Figure 4D appears to have two images which overlap. I also wish to note that the email address for the corresponding author does not seem to, um, correspond with their name. *** ![file] , 
 https: doi.org 10.1042 BSR-20194237_EOC The Editorial Office has been made aware of potential issues surrounding the scientific validity of this paper, hence has issued an expression of concern to notify readers whilst the Editorial Office investigates. , 
 #1 We are very sorry for our carelessness. We re-performed the Transwell assays and arranged new figure 4. , 
 #3 Iu2019m confused by the need to re-perform experiments. Was the original data no longer available? This is a 2020 paper. , 
 Retracted [4 May 2021](10.1042 BSR-20194237_RET). ------------------------------------ This Retraction follows an Expression of Concern relating to this article previously published by Portland Press. This article is being retracted from Bioscience Reports at the request of the Editor-in-Chief and the Editorial Board following receipt of a notification from a reader, alerting the Editorial Board to a duplicated image in Figure 4D. The authors were contacted regarding the concerns raised and provided a replacement images for Figures 2D, 3F and 4D, however given that the replacement images for Figure 2D also contained a new duplicated image, the Editorial Board stands by the decision to retract. The authors were contacted in regards to the retraction but did not respond. ,