Integrin β3/Akt signaling contributes to platelet-induced hemangioendothelioma growth
Authors: Yi Ren
Yijie Chi
Kwok-Fai So
Xiao-Ming Chen
Rui Gu
Xinhe Lai
Hongkai Xiang
Dale B Bosco
Xin Sun
Caixia Qin
Qishuang Zhou
Journal: Scientific Reports (2017)
Discipline: Multidisciplinary
Keywords: Hemangioendothelioma; angiogenesis; platelets; endothelial cells; proliferation
Affiliations: Institute of Inflammation and Diseases, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China., Guangdong-Hong Kong-Macau Institute of CNS Regeneration (GHMICR), Joint International Research Laboratory of CNS Regeneration Ministry of Education, Guangdong Medical Key Laboratory of Brain Function and Diseases, Jinan University, Guangzhou, China., Co-innovation Center of Neuroregeneration, Nantong, China., Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL, USA., Institute of Inflammation and Diseases, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. ., Guangdong-Hong Kong-Macau Institute of CNS Regeneration (GHMICR), Joint International Research Laboratory of CNS Regeneration Ministry of Education, Guangdong Medical Key Laboratory of Brain Function and Diseases, Jinan University, Guangzhou, China. ., Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL, USA. .

Abstract:  Hemangioendothelioma (HE) is a type of angiomatous lesions that features endothelial cell proliferation. Understanding the mechanisms orchestrating HE angiogenesis can provide therapeutic insights. It has been shown that platelets can support normal and malignant endothelial cells during angiogenesis. Using the mouse endothelial-derived EOMA cell line as a model of HE, we explored the regulatory effect of platelets. We found that platelets stimulated EOMA proliferation but did not mitigate apoptosis. Furthermore, direct platelet-EOMA cell contact was required and the proliferation was mediated via integrin β3/Akt signaling in EOMA cells. SiRNA knockdown of integrin β3 and inhibition of Akt activity significantly abolished platelet-induced EOMA cell proliferation in vitro and tumor development in vivo. These results provide a new mechanism by which platelets support HE progression and suggest integrin β3 as a potential target to treat HE. 

Retracted: No

PubPeer comments:
 Figure 6D, left part. * Yellow boxes: Two panels appear to show an overlap * (updated): Green boxes: Two panels appear to show an overlap ![file] , 
 #1 Dear Dr. Elisabeth M Bik, I am not the coauther of this paper. , 
 #2 Could you explain a little more? Was your name used without permission? Or are you saying the email address you used to contact you is incorrect? *** ![file] , 
 #3 The author (xiao-Ming Chen) presented in this paper and I are not the same one. I have no idea why my e-mail address was presented in this paper. Maybe, the author who submitted this manuscript can explain. , 
 #4 Dr. Chen, if you were included as an author on this paper without your knowledge, the authors have committed a serious wrong against you, especially as you are listed as corresponding author. The other corresponding author, Dr. Ren, has been informed of this exchange. Here s hoping this is not another misappropriation. I can add that from a platelet perspective, this paper has little merit since the platelets would simply have been activated in the experimental setup described. Hence there claim that Figure 4b shows  platelet engulfment is not tenable. I suggest you contact the journal and commence removal proceedings. , 
 #5 Hi, Dr. Salsola Zygophylla, Thanks for your suggestion. One of the coauthor (Xin Sun) have sent a e-mail to me about this mistake. The e-mail of Xiao-Ming Chen is chxmwz@163.com. They are contacting the journal to correct these issues. , 
 Thanks so much for taking the time and bringing attention to the errors in our paper. The first of these errors is the overlapping images and the second is the accidental input of the wrong email address for the corresponding author Xiao Ming Chen. In the latter case, we inputted the email of someone else with the exact same name when submitting the paper. We are currently in discussion with the journal to decide what the most appropriate and fair decision should be. Most importantly, we offer our sincerest apologies for our mistakes and oversight as it was never our intention to mislead any readers. We will ensure that something like never happens again. , 
 Correction, 29 September 2021: https: www.nature.com articles s41598-021-99149-4  *This Article contains an error in Figure 6, where the Control and Platelet images for Control siRNA and Integrin beta 3 siRNA groups were mistakenly taken from the same original image file in panel (d).* *The correct Figure 6 and accompanying legend appear below.* *Additionally, an incorrect email address for author Xiao-Ming Chen is quoted. Correspondence and requests for materials should be addressed to cxm@wmu.edu.cn.* ,