Circ_0005075 stimulates the proliferation and metastasis of glioma via downregulating SIRT1
Authors: D Liu
W-J Wang
J Liu
X-D Wang
F-Y Li
D-Z Fang
X-B Hui
Journal: European review for medical and pharmacological sciences (2020)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: circ_0005075; glioma progression; metastasis; prognosis; SIRT1
Affiliations: Department of Neurosurgery, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai'an, China. .

Abstract:  Objective: The aim of this study was to uncover the potential influence of circ_0005075 on the malignant progression of glioma and the underlying mechanism. Patients and methods: Circ_0005075 level in glioma tissues and cell lines was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The relation between circ_0005075 expression and metastasis of glioma patients was analyzed. Prognostic potential of circ_0005075 in glioma was assessed by calculating overall survival (OS) and progression-free survival (PFS). After knockdown or overexpression of circ_0005075, changes in the viability, migration, and wound closure percentage of T98-G and U87 cells were examined, respectively. Subsequently, expression pattern and prognostic value of SIRT1 in glioma patients were determined. Furthermore, the involvement of SIRT1 in glioma progression affected by circ_0005075 was evaluated through rescue experiments. Results: Circ_0005075 was significantly up-regulated in glioma tissues and cell lines. Meanwhile, its expression level was significantly higher in glioma patients with lymphatic metastasis or distant metastasis when compared with those with negative metastasis. OS and PFS were both remarkably worse in glioma patients with high expression level of circ_0005075. Knockdown of circ_0005075 decreased the viability, migration, and wound closure percentage of T98-G cells. However, overexpression of circ_0005075 in U87 cells yielded the opposite trends. SIRT1 expression level was negatively regulated by circ_0005075 in glioma. QRT-PCR results demonstrated that SIRT1 was significantly down-regulated in glioma tissues and cell lines. High level of SIRT1 predicted better prognosis of glioma patients. Rescue experiments confirmed that SIRT1 was responsible for the regulatory role of circ_0005075 in the malignant progression of glioma. Conclusions: Circ_0005075 is up-regulated in glioma tissues and correlated with distant metastasis and poor prognosis of glioma patients. Furthermore, it aggravates the malignant progression of glioma by down-regulating SIRT1. 

Retracted: No

PubPeer comments:
 Figure 4E. * Blue boxes: A panel in this figure appears to overlap with a panel in Figure 2C of WJ Wang et al., ERMPS (2020), DOI: 10.26355 eurrev_202001_20056 - https: pubpeer.com publications A93964B21F3EE8BAC2E56B992DA8F9, where it represents a different experiment. Can the authors please explain how two very different research groups can publish a photo of the same specimen? ![file] , 
 On one hand...  A total of **40** matched glioma tissues and para-cancerous tissues were surgically resected from glioma patients. ... By analyzing the clinical data of enrolled **40** glioma patients,.. On the other hand... ![file] I am loath to belabour the obvious, but 40 34. , 
 Figure 1 (n = 34).  Upregulation of circ_0005075 in glioma tissues and cell lines.... Circ_0005075 level in glioma patients either with lymphatic metastasis or not. D, Circ_0005075 level in glioma patients either with distant metastasis or not. **E, Overall survival of glioma patients with high and low level of circ_0005075.** F, Progression-free survival of glioma patients with high and low level of circ_0005075. ![file] Fig 3F.  Overall survival of glioma patients with high and low level of SIRT1 . ![file] Patients are either almost all dead by 55 months, or by 75 months, depending on whether they are subgrouped by circ_0005075 or by SIRT1 , 
 #3 *[left]* Fig 1 from [Fang et al (2020)] . *[right]* Fig 1 from [Hou et al (2018)] . ![file] , 
 *[left]* Fig 3E from [Fang et al (2020)] .  A negative correlation between expression levels of circ_0005075 and SIRT1 in glioma tissues. *[right]* Fig 3E from [Li, Li Liu (2019)] .  A significant negative correlation between miR-328-3p and MMP-9 expression levels in the hepatocellular carcinoma tissues . ![file] No comment required. , 
 Dear Sir.Thank you for your comments.I will check the original data and contact the editor to correct our data. Thank you very much. ,