Liver receptor homolog 1 controls the expression of carboxyl ester lipase
Authors: Johan Auwerx
Kristina Schoonjans
Elisabeth Fayard
Jean-Sébastien Annicotte
Journal: Journal of Biological Chemistry (2003)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: LRH-1; cholesterol homeostasis; CEL; pancreas; gene expression
Affiliations: Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS/INSERM/Université Louis Pasteur, B.P. 10142, F-67404 Illkirch, C.U. de Strasbourg, France.

Abstract:  The orphan nuclear receptor liver receptor homolog 1 (LRH-1) plays a central role in cholesterol homeostasis by regulating a number of hepatic and intestinal genes critical for reverse cholesterol transport and bile acid homeostasis. Herein, we describe the identification of carboxyl ester lipase (CEL) as a novel target of LRH-1 in pancreas, a tissue in which LRH-1 is abundantly expressed. In situ hybridization and gene expression studies demonstrate that both LRH-1 and CEL are co-expressed and confined to the exocrine pancreas. LRH-1 interacts with a consensus LRH-1 response element in the human CEL promoter, which is perfectly conserved in the rat gene, and induces CEL promoter activity in cotransfection assays. As reported for other LRH-1 target genes, the nuclear receptor short heterodimer partner represses LRH-1-induced CEL promoter activity. Chromatin immunoprecipitation demonstrates that binding of LRH-1 to the CEL promoter increases histone H4 acetylation corresponding with the activation of endogenous CEL gene transcription. Our data, identifying CEL as the first pancreatic LRH-1 target gene, indicate that LRH-1 is an important player in enterohepatic cholesterol homeostasis. 

Retracted: No

PubPeer comments:
 This paper appears to belong to a set of (as of today) seven papers that all show very similar, and sometimes overlapping, panels with Alizarin Red-stained cells. These papers were published by different authors at different hospitals, suggesting the photos all might have originated from the same source. The papers are: * AG Gao et al., , The First Affiliated Hospital Of Soochow University, Suzhou, DOI: 10.26355 eurrev_201807_15527- https: pubpeer.com publications 65A5976CDD42166D927AF2D3BA0B56 * B Luo et al., The Affiliated Hospital (Traditional Chinese Medicine) of Southwest Medical University, Luzhou, DOI: 10.26355 eurrev_201908_18717 - https: pubpeer.com publications 20DFC925FC185C52D8BA99B153F510 * Changgong Lan et al., The First Affiliated Hospital of Jinan University, Guangzhou, DOI: 10.3892 etm.2019.8246 - https: pubpeer.com publications 1D79EA9F787AF36661079482544D12 * FB Weng et al., The Ninth Peopleu2019s Hospital of Suzhou, Suzhou, DOI: 10.26355 eurrev_201912_19759 - https: pubpeer.com publications D63F002727B3A990A4571F7D4B6F56 * GC Gao et al., The Second Affiliated Hospital of Nanchang University, Nanchang, DOI: 10.26355 eurrev_202001_20029 - https: pubpeer.com publications F04C25EBF6963C260938DF3B6BB999 * JY Li et al., Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, DOI: 10.26355 eurrev_201908_18519 - https: pubpeer.com publications 6171C86828BF72676BB2B178610093 * YC Fu et al., Health Management Center, Weifang Peopleu2019s Hospital, Weifang, DOI: 10.26355 eurrev_201908_18632 - https: pubpeer.com publications FF66C46D23036BC26668481C279E4B Here is a collage of the images from these papers. Boxes of the same color highlight panels or parts of panels that look similar. ![file] ,