Retracted : MicroRNA‐21 antisense oligonucleotide improves the sensitivity of A375 human melanoma cell to Cisplatin: An in vitro study
Authors: Ang Zeng
Hai-Lin Zhang
Ming Bai
Zheng Qi
Fei Long
Lou-Bin Si
Ru Zhao
Journal: Journal of Cellular Biochemistry (2018)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Cisplatin; IC50; antisense oligonucleotide; drug resistance; melanoma
Affiliations: Department of Plastic and Reconstructive Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, P.R. China.

Abstract:  This study explored Cisplatin resistance effect of microRNA-21 (miR-21) antisense oligonucleotide (AS-ODN) in human melanoma A375 cell. AS-ODN was transfected in melanoma A375 cells and Cisplatin-resistant cell line A375/CDDP, and divided into the AS-ODN, nonsense oligonucleotide (NS-ODN) and normal groups. Cell ultrastructure changes were observed through transmission electron microscope. MiR-21 AS-ODN could be tested cell growth effect in different time periods by trypan blue exclusion. MiR-21 mRNA expression change was detected by quantitative fluorescence PCR. Cell apoptosis, cycle distribution and miR-21 AS-ODN effect on proliferation and Cisplatin sensitivity were tested by flow cytometry, MTT assay, TUNEL, and Clonogenic assay. Cell apoptosis was observed after transfection 24 h with the AS-ODN group, while the NS-ODN and normal group cells had no apoptotic symptoms; Compared with the normal group, the AS-ODN group began to show obvious cell growth inhibition effect after transfection 24 h lasting 72 h (all P &lt; 0.05), but the NS-ODN group had no significant difference (P &gt; 0.05). miR-21 mRNA expression in the AS-ODN group was obviously decreased with rising apoptosis rate (all P &lt; 0.05) and there was no significant difference in the NS-ODN group (P &gt; 0.05). MiR-21 AS-ODN could remarkably increase A375 cell and A375/CDDP cell sensitivity to Cisplatin (P &lt; 0.05), while A375 cell sensitivity to Cisplatin between the NS-ODN group and the normal group had no difference. MiR-21 AS-ODN decreased IC50 and increased Cisplatin sensitivity for A375 cells and A375/CDDP cells, which would be a new target of melanoma treatment. 

Retracted: Yes

PubPeer comments:
  Fig. 3. Comparisons of ultrastructural changes of the A375 and A375 CDDP cells after transfection of 24h in the normal, AS-ODN, and NS-ODN groups A, transmission electron microscopy of cell morphology 0 h after transfection; B, transmission electron microscopy of cell morphology 24 h after transfection; C, transmission electron microscopy of cell morphology 48 h after transfection; D, transmission electron microscopy of cell morphology 72 h after transfection . Surely there has been some mistake. The 24-hour A375 CDDP and 48-hour A375 images are the same, apart from a 90-deg rotation and a nonlinear shear transformation of some kind. The 48-hour A375 CDDP and 72-hour A375 images are also the same, apart from a 90-deg rotation and a smaller shear. ![file] , 
 Fig 1B,  Comparisons of miR-21 expression in the A375 and A375 CDDP cells in the normal, AS-ODN, and NS-ODN groups ... B, expression of miR-21 in A375 cells in each groups 24 h, 48 h, and 72 h after transfection . It is not clear which of the three time delays provided the example of a Western Blot. Shown at upper left. At right, for comparison, Fig 6C from [Wen et al (2018)] and Fig 5B from [Wu et al (2018)] . Other manifestations of the same versatile loading control are adduced in those threads. ![file] , 
 Retracted [30 June 2021] . ----------------------------------- The above article, published online on 23 October 2017 in Wiley Online Library has been retracted by agreement between the the journal s Editor in Chief, Prof. Dr. Christian Behl, and Wiley Periodicals LLC. The retraction has been agreed following an investigation based on allegations raised by a third party. A detailed investigation revealed that several image elements of the experimental data were published elsewhere in a different scientific context. Thus, the editors consider the conclusions of this article to be invalid. ,