Resveratrol Exerts Dosage-Dependent Effects on the Self-Renewal and Neural Differentiation of hUC-MSCs
Authors: Fangxia Guan
Qu Xing
Bo Yang
Shanshan Ma
Qinghua Li
Yanting Zhang
Jishi Song
Kang Han
Kun Zhang
Xinxin Wang
Nan Meng
Ning Yao
Journal: Molecules and cells (2016)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: SIRT1; human umbilical cord derived mesenchymal stem cells; neural differentiation; resveratrol; self-renewal.
Affiliations: The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China., School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.

Abstract:  Resveratrol (RES) plays a critical role in the fate of cells and longevity of animals via activation of the sirtuins1 (SIRT1) gene. In the present study, we intend to investigate whether RES could promote the self-renewal and neural-lineage differentiation in human umbilical cord derived MSCs (hUC-MSCs) in vitro at concentrations ranging from 0.1 to 10 μM, and whether it exerts the effects by modulating the SIRT1 signaling. Herein, we demonstrated that RES at the concentrations of 0.1, 1 and 2.5 μM could promote cell viability and proliferation, mitigate senescence and induce expression of SIRT1 and Proliferating Cell Nuclear Antigen (PCNA) while inhibit the expression of p53 and p16. However, the effects were reversed by 5 and 10 μM of RES. Furthermore, RES could promote neural differentiation in a dose-dependent manner as evidenced by morphological changes and expression of neural markers (Nestin, βIII-tubulin and NSE), as well as pro-neural transcription factors Neurogenin (Ngn)1, Ngn2 and Mash1. Taken together, RES exerts a dosage-dependent effect on the self-renewal and neural differentiation of hUC-MSCs via SIRT1 signaling. The current study provides a new strategy to regulate the fate of hUC-MSCs and suggests a more favorable in vitro cell culture conditions for hUC-MSCs-based therapies for some intractable neurological disorders. 

Retracted: No

PubPeer comments:
 Figure 3C appears to have some figure overlap, although the magnification seems different, despite the scale being described as the same. *** Closeup ![file] *** Full figure ![file] , 
 Dear Dr. Biskrensis,u00a0 Thanks very much for pointing out this mistake. Our group have conducted an investigation and checked the raw data and found that pictures in Figure3C were accidentally misplaced (CON and 2.5u03bcM). The corrected Figure3C was attached below. Meanwhile, the percentage of senescent cells was reanalyzed in the updated Figure3D. In addition, we provide pictures from three independentu00a0experiments to confirm the results. The correction did not affect the conclusions of the paper. We are contacting the editorial office of the journal for correction. As the first author, I am deeply sorry about the unintentional error on my part and sincerely apologize for this. Thank you again for the comment, we will definitely establish a more careful and rigorous attitude towards scientific work and take strict measures to avoid such mistake in the future. Best regards, Xinxin Wang! [file] ![file] ![file] ![file] , 
 #1 Dear Dr. Biskrensis,u00a0 Thanks very much for pointing out this mistake. Our group have conducted an investigation and checked the raw data and found that pictures in Figure3C were accidentally misplaced (CON and 2.5u03bcM). The corrected Figure3C was attached below. Meanwhile, the percentage of senescent cells was reanalyzed in the updated Figure3D. In addition, we provide pictures from three independentu00a0experiments to confirm the results. The correction did not affect the conclusions of the paper. We are contacting the editorial office of the journal for correction. As the first author, I am deeply sorry about the unintentional error on my part and sincerely apologize for this. Thank you again for the comment, we will definitely establish a more careful and rigorous attitude towards scientific work and take strict measures to avoid such mistake in the future. Best regards, Xinxin Wang![file] ![file] ![file] ![file] , 
 #3 Thank you for your reply. For my own education, can you explain how the image magnification came to be different? ,