miR-125a-5p upregulation suppresses the proliferation and induces the cell apoptosis of lung adenocarcinoma by targeting NEDD9
Authors: Jiachen Shi
Guojun Zhang
Jianbo Wu
Qianqian Sun
Guoqiang Zhao
Chunya Lu
Hong Zheng
Yuanyuan Wang
Journal: Oncology Reports (2017)
Discipline: CLINICAL MEDICINE
Keywords: MicroRNAs; miR-125a-5p; lung adenocarcinoma; qRT-PCR assay; lymph node metastases
Affiliations: Department of Pathophysiology, Institute of Molecular Medicine, Medical College of Henan University, Kaifeng, Henan 475004, P.R. China., School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China., Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.

Abstract:  MicroRNAs (miRNAs) are critical translational regulators that act as oncogenes or tumor-suppressor genes. qRT-PCR assay results showed that the expression levels of miR-125a-5p are lower in lung adenocarcinoma (AD) tissues than expression levels in adjacent non-neoplastic tissues. This relative expression was found to be significantly associated with lymph node metastases. Cell growth, apoptosis, caspase activity and Transwell invasion assay results showed that in two lung adenocarcinoma cell lines transfected with a miR-125a-5p mimic, proliferation and invasion rates were found to be significantly reduced, whereas the apoptosis rate of the miR-125a-5p mimic group was enhanced. Subsequent western blotting and luciferase reporter assays showed that miR-125a-5p is able to bind to putative binding sites within the mRNA 3' untranslated region (UTR) of neural precursor cell expressed, developmentally downregulated 9 (NEDD9). Our findings suggest that miR-125a-5p may serve as a therapeutic agent for lung adenocarcinoma through its major target, NEDD9. 

Retracted: No

PubPeer comments:
 Top left:  Figure 3. Effect of miR-125a-5p mimic on the invasive capacity of A549 and H1299 cells using Transwell assay. (A) The Transwell images of A549 and H1299 cell lines. Top right:  Figure 3. Effects of upregulation of miR-1207-5p on apoptosis and invasion of EC9706 and EC-1....(B) Transwell assays were used to assess the invasive ability. From  miRNA-1207-5p is associated with cancer progression by targeting stomatin-like protein 2 in esophageal carcinoma [(Yang et al 2015)] . Areas of matching cell colonies marked with colored rectangles, and paired up along the bottom. Note, though that there are occasional colonies in the 2017 versions that are not present in the 2015 versions, as if something has happened to the images as well as accidental reuse. ![] , 
 Top left:  Figure 3. Effect of miR-125a-5p mimic on the invasive capacity of A549 and H1299 cells using Transwell assay. (A) The Transwell images of A549 and H1299 cell lines. Top right:  Figure 5. The overexpression of miR-519d restricted cell invasion in A549 and H1299 cells. The number of cells penetrating the transwell membrane in the miR-519d agomir group ... From  Overexpression of miR-519d in lung adenocarcinoma inhibits cell proliferation and invasion via the association of eIF4H [(Bai et al 2017)] . ![] Areas of matching cell colonies marked with colored rectangles, and paired up at bottom right. Note, though that there are occasional **repeated** colonies in Zheng s versions that are not present in Bai s versions, as if something has happened to the images as well as accidental reuse. , 
 [Perhaps a systemic problem] . Journals and readers may wish to proceed with caution. ,