MiR-HCC2 Up-regulates BAMBI and ELMO1 Expression to Facilitate the Proliferation and EMT of Hepatocellular Carcinoma Cells
Authors: Yajie Fan
Hong Wang
Shengping Li
Hong Xie
Min Liu
Huijie Gao
Jianying Yi
Le Zhang
Hua Tang
Ting Mu
Journal: Journal of Cancer (2019)
Discipline: CLINICAL MEDICINE
Keywords: BAMBI; ELMO1; HCC; invasion; miRNAs
Affiliations: Tianjin Life Science Research Center and Department of Pathogen Biology, Collaborative Innovation Center of Tianjin for Medical Epigenetics, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China., State Key Laboratory of Oncology in Southern China, Department of Hepatobiliary Oncology, Cancer Center, Sun Yat-sen University, 651 Dong-Feng Road East, Guangzhou 510060, China.

Abstract:  MicroRNAs (miRNAs) are a class of gene expression regulators that participate in the occurrence and development of hepatocellular carcinoma (HCC), although the underlying mechanism by which they function in HCC has not been fully elucidated. Here, small RNA deep sequencing was used to identify aberrantly expressed miRNAs in HCC tissues, and a novel miRNA named miR-HCC2 was identified. RT-qPCR analysis demonstrated that miR-HCC2 displayed higher expression in HCC tissues than in adjacent non-tumor tissues. We documented that miR-HCC2 facilitated the growth, migration and invasion of HCC cells by accelerating cell cycle progression, incressing the expression of epithelial-to-mesenchymal transition (EMT)-associated marker vimentin but decreasing the expression of E-cadherin. MiR-HCC2 directly targeted the 3' UTR of BAMBI and ELMO1 and up-regulated their expression. Both BAMBI and ELMO1 had the same patterns of expression with miR-HCC2 in HCC tissues. Additionally, blocking BAMBI or ELMO1 counteracted the phenotypic alterations elicited by miR-HCC2. Collectively, our investigation identified miR-HCC2 as a new positive modulator of HCC aggressiveness that may serve as a potential biomarker for the development of diagnostic and therapeutic approaches for HCC. 

Retracted: No

PubPeer comments:
 [March 2022] . -------------------------------------------- Retraction of six ARVO Annual Meeting abstracts: u201cEffect of 3-Hour Normobaric Hyperoxia on Diabetic Macular Edemau201d by Robert Minturn, Brendan Seto, Keiko Yamada, Ke Zeng, and Jorge G. Arroyo ([Invest Ophthalmol Vis Sci. 2019;60(9):2635] ). u201cThe Effect of Hyperoxia and Hypercapnia on Retinal Vascular Blood Flow in Healthy Adultsu201d by Keiko Yamada, Brendan Seto, Christopher Llerena, Christopher Hsu, Chie Sotozono, Takatoshi Maeno, and Jorge G. Arroyo ([Invest Ophthalmol Vis Sci. 2019;60(9):5742] ). u201cHuman Laser Speckle Retinal Blood Flow Changes Associate with Exposure to Varying Concentrations of O2 and CO2u201d by Keiko Yamada, Rachelle Koch, Purva Atreay, Sinjin Swartz, Takatoshi Maeno, Chie Sotozono, Brendan Seto, and Jorge G. Arroyo ([Invest Ophthalmol Vis Sci. 2020;61(7):1749] ). u201cShort-Term Normobaric Hyperoxia Treatment for Dry Age-Related Macular Degeneration: An Exploratory Studyu201d by Justin Wang, Colin Lemire, Brendan Seto, Keiko Yamada, and Jorge G. Arroyo ([Invest Ophthalmol Vis Sci. 2021;62(8):312] ). u201cAnatomical and Physiological Effects of Nocturnal Normobaric Hyperoxia Treatment in Patients with Diabetic Macular Edemau201d by Soobin Song, Colin Lemire, Purva Atreay, Brendan Seto, Keiko Yamada, and Jorge G. Arroyo ([Invest Ophthalmol Vis Sci. 2021;62(8):1069] ). u201cAssessing Clinical and Anatomical Outcomes for Central Serous Chorioretinopathy Treated With Standard of Care or Experimental Hyperoxiau201d by Mortada Salman Najem, Colin Lemire, Abigail Ralph, Brendan Seto, Keiko Yamada, and Jorge G. Arroyo ([Invest Ophthalmol Vis Sci. 2021;62(8):3105] ). After the research outlined in the above abstracts was presented at the ARVO Annual Meetings, and after the abstracts were published in IOVS, a serious issue related to lack of Institutional Review Board (IRB) approval for the research procedures was brought to the attention of the journal by the IRB, and has been corroborated by the corresponding author. It was therefore agreed that the abstracts should be retracted from the journal. ,