<p>LncRNA MALAT1 Promotes Survival of Epithelial Ovarian Cancer Cells by Downregulating miR-145-5p</p>
Authors: Ke Wang
Yi-Min Wang
Ye Zhao
Journal: Cancer Management and Research (2020)
Discipline: CLINICAL MEDICINE
Keywords: EOC; MALAT1; cell survival; miR-145-5p.
Affiliations: Department of Gynaecology and Obstetrics, China-Japan Union Hospital of Jilin University, Changchun 130000, Jilin, People's Republic of China., Department of Dermatology, China-Japan Union Hospital of Jilin University, Changchun, Jilin, 130000, People's Republic of China., Central Research Room, China-Japan Union Hospital of Jilin University, Changchun 130000, Jilin, People's Republic of China.

Abstract:  Purpose: This paper was aimed at investigating the regulatory mechanism of long non-coding RNA metastasis-associated lung adenocarcinoma transcript-1 (MALAT1) in epithelial ovarian cancer (EOC). Materials and methods: MALAT1 and miR-145-5p expression in the tissues, serum, and EOC cell lines (TOV-112D, TOV-21G) of patients with EOC were detected. The two genes were transfected into the cells via upregulating or downregulating their expression. Levels of apoptosis-related proteins (Caspase-3, Bax, Bcl-2) were analyzed. Mechanisms of cell proliferation, invasion, and apoptosis were studied. Results: MALAT1 was high expressed in EOC tissues, while miR-145-5p was poorly expressed in them. The areas under the curves (AUCs) of the two genes for diagnosing EOC were greater than 0.850, and the two had a significantly negative correlation. According to multivariate Cox regression analysis, high MALAT1 expression, tumor size, degree of differentiation, case staging, and lymph node metastasis were the independent risk factors affecting prognosis. The 5-year overall survival rate (OSR) of patients with low MALAT1 expression was remarkably higher than that of those with high expression. Overexpressing miR-145-5p and silencing MALAT1 could inhibit EOC cells from proliferating and invading, increase their apoptotic rate, and improve levels of the apoptosis-related proteins. After co-transfection with MALAT1-inhibitor + miR-145-5p-inhibitor, the proliferation and invasion of TOV-112D and TOV-21G cells were inhibited and the apoptotic rate rose more obviously. Inhibiting MALAT1 could increase miR-145-5p expression, thus inhibiting EOC cells from proliferating and invading and thereby increasing their apoptotic rate. Conclusion: MALAT1 promotes EOC cells' survival by downregulating miR-145-5p so it may become a new direction for EOC diagnosis and gene therapy. 

Retracted: Yes

PubPeer comments:
 Figs 4I, 5I.  Flow cytometry maps . ![file] Fig 7E.  Flow cytometry maps. ![file] Each panel contains a halo of regularly-spaced dots, surrounding a central clump with a curious texture, almost woven in appearance (also visible in [Qu et al 2020] ). Perhaps these are instrumentation artefacts of some kind. Is it possible for the authors to upload the raw data files to a data repository somewhere? , 
  Glioma tissues and normal brain tissues were taken out from the liquid nitrogen container for grinding . This is a curious statement to encounter in a paper that primarily focusses on epithelial ovarian cancer. Was the manuscript originally written for a different purpose? According to the test, 105 patients with EOC were recruited. Table 1 vacillates between 54 high- and 54-low-expression cases, i.e. 108; or 104 in the factor subgroupings (except for tumor size where there are 108 cases again). The subgrouped numbers never add up to 54 high- or 54 low-expression. ![file] , 
 Could the authors also please provide high resolution images of Figure 5J? Uncropped originals would be preferred. The images I have found online seem to contain unusual halos around the smears. You may post those here or use a link to an external site (such as Imgur.com or Google Drive) for larger files. Thank you! *** ![file] , 
 Retracted, [17 September 2021] . ------------------- The Editor and Publisher of Cancer Management and Research wish to retract the published article. Concerns were raised over the flow cytometry maps and western blot data presented in this article. There also appeared to be errors in the tumor size numbers (n = 108) reported in Table 1, where the subgroups of High and Low MALATI expression do not add up to 54 as reported. The authors responded to our queries but were unable to provide the original raw data for their study and have therefore agreed to retract the article. Our decision-making was informed by our policy on publishing ethics and integrity and the COPE guidelines on retraction. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as u201cRetractedu201d. ,