miR-130a alleviates neuronal apoptosis and changes in expression of Bcl-2/Bax and caspase-3 in cerebral infarction rats through PTEN/PI3K/Akt signaling pathway
Authors: Liang Kong
Chaosheng Li
Meiqi Di
Shuhua Gui
Tinggang Wang
Junquan Gu
Yinming Wang
Linlin Hu
Journal: Experimental and Therapeutic Medicine (2020)
Discipline: CLINICAL MEDICINE
Keywords: PTEN/PI3K/Akt signaling pathway; apoptosis; cerebral infarction; miR-130a.
Affiliations: Department of Neurology, The Third People's Hospital of Wuxi, Wuxi, Jiangsu 214041, P.R. China.

Abstract:  Effect of micro ribonucleic acid (miR)-130a on neuronal apoptosis in rats with cerebral infarction (CI) was studied to explore whether phosphatase and tensin homolog deleted on chromosome ten (PTEN)/phosphatidylinositol 3-hydroxy kinase (PI3K)/protein kinase B (Akt) is involved in the regulation of neuronal apoptosis. Thirty-six Sprague-Dawley (SD) rats were randomly divided into blank control group, model group and miR-130a low-expression group. miR-130a was determined by quantitative polymerase chain reaction (qPCR), the content of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-10 was detected using the enzyme-linked immunosorbent assay (ELISA) kits, and the neuronal apoptosis level in each group was determined through terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. The neurobehavioral score was significantly lower in model group than that in blank control group (P&lt;0.01), while it was significantly higher in miR-130a low-expression group than that in model group (P&lt;0.01). Compared with blank control group, the model group had obviously increased content of TNF-α and IL-6 (P&lt;0.01), decreased content of IL-10 (P&lt;0.01), more apoptotic neurons (P&lt;0.01), higher expression of caspase-3 (P&lt;0.01), and obviously lower Bcl-2/Bax (P&lt;0.01). Moreover, expression of phosphorylated (p)-PTEN, PI3K and p-Akt in brain tissues was remarkably lower in the model group than those in the blank control group (P&lt;0.01). The expression level of miR-130a in brain tissues of CI rats is significantly increased. miR-130a promotes the release of inflammatory factors and facilitates neuronal apoptosis through suppressing the PTEN/PI3K/Akt signaling pathway. 

Retracted: No

PubPeer comments:
 Figure 4.  Neuronal apoptosis level in each group detected through TUNEL staining. (A) Micrograph . Below, Fig 4 from [Li Wang (2019)] ,  Osteocyte apoptosis level detected via TUNEL staining. A, TUNEL staining . ![file] , 
 At left here, Figure 5A from [Wang et al (2020)] .  Expression levels of apoptosis-related proteins in CI tissues in each group. (A) Protein bands . Middle, Figure 6A from [Yu et al (2019)] .  Expressions of apoptosis-related proteins in each group measured by Western blotting . At right, Figure 6A from [Li Wang (2019)] :  Expression levels of BMP signaling pathway-related proteins detected via Western blotting. A, Protein band . ![file] Unexpected similarities. ![file] Unexpected similarities. , 
 At right, Figure 6A:  Expression levels of PTEN PI3K Akt pathway-related proteins in brain tissues in each group. (A) Protein bands . At left, Figure 7A from [Zhou et al (2020)] : Expression levels of PI3K AKT signaling pathway-related proteins. A, Protein bands . ![file] Unexpected similarity. ,