Effects of cancer-testis antigen, TFDP3, on cell cycle regulation and its mechanism in L-02 and HepG2 cell lines in vitro
Authors: Yunshen Jiao
Jiarui Kang
Xi Chen
Likai Gao
Lingyu Ding
Ming Chu
Huanhuan Li
Tieshan Wang
Zirui Gao
Yuedan Wang
Xiaofan Zhao
Journal: PLoS ONE (2017)
Discipline: Multidisciplinary
Keywords: TFDP3; cancer-testis antigens; drug resistance; apoptosis; cell cycle
Affiliations: Department of Immunology, School of Basic Medical Science, Peking University, Beijing, China., Key Laboratory of Medical Immunology, Ministry of Health, Beijing, China., Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China., Department of Pathology, the First Affiliated Hospital of General Hospital of Chinese People's Liberation Army, Beijing, China.

Abstract:  TFDP3, also be known as HCA661, was one of the cancer-testis antigens, which only expressed in human tissues. The recent researches about TFDP3 mostly focused on its ability to control the drug resistance and apoptosis of tumor cells. However, the role of TFDP3 in the progress of the cell cycle is rarely involved. In this study, we examined the expression of TFDP3 in human liver tissues firstly. After that, we detect the expression of TFDP3 at the RNA level and protein level in L-02 cell line and HepG2 cell line, and the location of TFDP3 was defined by immunofluorescence technique. Furthermore, we synchronized the cells to G1 phase, S phase and G2 phase, and arrested cell mitosis. The localization of TFDP3 and co-localization with E2F1 molecules in different phases of hepatocyte lines. Finally, TFDP3 gene knockout was performed on L-02 and HepG2 cell lines, and detected the new cell cycles by flow cytometry. The result showed that the expression of TFDP3 molecule is negative in normal liver tissue, but positive in immortalized human hepatocyte cell line, and the expression level is lower than in hepatocellular carcinoma cell line. The expression level of TFDP3 was in the dynamic change of L-02 and HepG2 cell lines, and was related to the phase transition. TFDP3 can bind to E2F1 molecule to form E2F/TFDP3 complex; and the localizations of TFDP3 and E2F1 molecules and the co-localization were different in different phases of cell cycle in the nucleus and cytoplasm, which indicated that the E2F/TFDP3 complex involved in the process of regulating the cell cycle. By knocking down the TFDP3 expression level in L-02 and HepG2 cell lines, the cell cycle would be arrested in S phase, which confirmed that TFDP3 can be a potential target for tumor therapy. 

Retracted: No

PubPeer comments:
 I ve tried to access the S1 File  The raw data of some figures and tables in the paper. For example, the supplemental files for Fig 3B as indicated by blue arrows in the image below. However, when I click on the files, it simply links me back to the article Figure itself. The Figure 3B in the article, and incidentally that linked to by the supplemental files is presented below. This is true for the other supplemental files as well, including all other western blot figures. Doo the authors have the raw uncropped blot of Fig 3B? Would they be please willing to share it here or fix the link on the article site? ![file] ![file] There is also a .rar compressed folder included in the supplementary info, which contains these files. HOWEVER, these are not labelled, and some contain possibly duplicated images. For example, when opening the compressed folder, I am presented with this list of files: ![file] I can t match them up to those listed in the paper, because the files in the compressed folder are listed as Figure A, Figure B, Figure C, etc... but are not categorized by Figure 1 A, Figure 2 A, etc.... so what is Figure A supposed to represent? Also there appears to be possible duplication in the compressed raw western blot images. For example (the green and orange boxes are mine, they indicate to the pubpeer reader which blots appear to be duplicated between the different figures) ![file] ,