Effects of microRNA-183 on epithelial-mesenchymal transition, proliferation, migration, invasion and apoptosis in human pancreatic cancer SW1900 cells by targeting MTA1
Authors: Xizhou Lin
Bujian Pan
Jun Xiao
Xiaodan Jin
Haibo Yu
Haichuan Chen
Liang Zheng
Hongliang Song
Journal: Experimental and Molecular Pathology (2017)
Discipline: CLINICAL MEDICINE
Keywords: Epithelial-mesenchymal transition; Invasion; MTA1; Migration; Pancreatic cancer
Affiliations: Department of Digestive Diseases, Wenzhou People's Hospital, The Third Clinical College of Wenzhou Medical University, Wenzhou 325000, PR China., Department of Surgery, Wenzhou Central Hospital, The Dingli Clinical Institute of Wenzhou Medical University, Wenzhou 325000, PR China., Department of Surgery, Wenzhou Central Hospital, The Dingli Clinical Institute of Wenzhou Medical University, Wenzhou 325000, PR China. Electronic address: .

Abstract:  Objective: This study aims to explore effects of miR-183 on epithelial-mesenchymal transition (EMT) and invasion by targeting MTA1 in human pancreatic cancer (PC) cells. Methods: Totally, 108 PC patients admitted in Wenzhou Central Hospital and Wenzhou People's Hospital, The Dingli Clinical Institute of Wenzhou Medical University from March 2010 to March 2014 were enrolled. qRT-PCR and immunohistochemistry were applied to examine expression of MTA1 mRNA and protein. Samples were divided into 6 groups: blank, NC, miR-183 mimics, miR-183 inhibitors, MTA1-siRNA and miR-183 inhibitors +MTA1-siRNA groups. CCK8 method was employed for determining cell proliferation rate, flow cytometry for cell apoptosis rate, scratch test for cell migration and Transwell assay for cell invasion. qRT-PCR and Western blotting were used to determine expression of MTA1, E-cadherin and Vimentin mRNA and protein. Results: Positive expression rate of MTA1 was upregulated in PC tissues, and expression of miR-183 and MTA1 was associated with differentiation, migration, tumor size, TNM. The miR-183 mimics and MTA1-siRNA groups showed a decrease in proliferation, migration and invasion, whereas increased apoptosis, in comparison with blank and NC groups, as expression of MTA1 and Vimentin mRNA and protein were reduced, expression of E-cadherin mRNA and protein was elevated. Compared to blank and NC groups, the miR-183 inhibitors group exhibited enhanced proliferation, migration and invasion and inhibited apoptosis; increased expressions of MTA1 and Vimentin mRNA and protein and decreased expressions of E-cadherin mRNA and protein. Conclusion: Our study supported that miR-183 could repress EMT and invasion of human PC cells through inhibition of MTA1 expression. 

Retracted: No

PubPeer comments:
 Fig 5A:  Comparisons on protein expressions of MTA1, E-cadherin and Vimentin in transfected PC SW1990 cells among different groups. Note: A, Western blotting image for protein expressions . What the chances of finding a distinctive protein blob also appearing in Fig 3B of [Wu et al (2019)] *[at right]*? ![file] , 
 Fig 9A,  Comparisons on cell invasion capacity in human PC SW1990 cells among different groups after transfection through transwell assy. Note: A, cell invasion conditions in different groups . ![file] Fig 8C of [Li et al (2017)] : ![] Different cells, different treatments, different authors, same panel. , 
 Fig 7A.  Comparisons on cell apoptosis and cell cycle distribution in PC SW1990 cells among different groups after transfection with Annexin V PI double parameter method. Note: A, apoptosis conditions of PC SW1990 cells among different groups assessed by flow cytometry . ![file] Blue and orange boxes mark similarities in the upper left quadrants between these  sneezing dust bunnies and, for instance, those in Fig 6A of [Zhu et al (2018)] , with more manifestations in that thread: ![] The magenta box calls attention to a panel in Fig 6 of [Ao et al (2017)] . where again the upper left quadrant has a surprisingly similar constellation of points. ![file] , 
 With their published corrigendum of December 2020, Experimental and Molecular Pathology and their publisher Elsevier acually accept falsification of research data. https: www.sciencedirect.com science article pii S001448002030842X , 
 #4  The authors regret that their article u201cEffects of microRNA-183 on epithelial-mesenchymal transition, proliferation, migration, invasion and apoptosis in human pancreatic cancer SW1900 cells by targeting MTA1u201d that has been published in Experimental and Molecular Pathology had some errors. We recently found that in the Western Blot experiment in Fig. 5, the wrong marker was selected due to the nonstandard operation of the experiment. We conducted the experiment again and provided the correct Western Blot results. In addition, Fig. 7A and Fig. 9 had some mistakesuff0cthe corrected figures appear below. The authors would like to apologise for any inconvenience caused. ![file] ![file] ![file] ,