Long noncoding RNA LUCAT1 promotes migration and invasion of prostate cancer cells by inhibiting KISS1 expression
Authors: Y-S Cui
Y-Q Wang
C Liu
Y-W Li
L Wang
S Liu
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: lncRNA; LUCAT1; prostate cancer; RT-qPCR; overall survival
Affiliations: Department of Urology, Qilu Hospital of Shandong University, Jinan, China. .

Abstract:  Objective: Recent researches have revealed the role of long noncoding RNAs (lncRNAs) in tumor development. In this study, the potential function of lncRNA LUCAT1 in the progression of prostate cancer was identified. Patients and methods: Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was used to detect LUCAT1 expression in both prostate cancer cells and tissue samples. Moreover, the association between LUCAT1 expression level and overall survival of prostate cancer patients was analyzed. In addition, wound healing assay and transwell assay were conducted to evaluate the regulatory effect of LUCAT1 on prostate cancer cells. Furthermore, the underlying mechanism of LUCAT1 in regulating the development of prostate cancer was explored via qRT-PCR and Western blot. Results: LUCAT1 expression was much higher in prostate cancer samples than controls. Besides, LUCAT1 expression was correlated with the overall survival of prostate cancer patients. Moreover, LUCAT1 overexpression promoted in vitro migration and invasion of prostate cancer cells. In addition, the mRNA and protein expression of KISS1 were downregulated after LUCAT1 overexpression. Furthermore, it was found that the expression level of KISS1 was negatively related to the expression level of LUCAT1 in prostate cancer tissues. Conclusions: LUCAT1 could enhance migration and invasion of prostate cancer cells by regulating KISS1, which might offer a potential therapeutic target for prostate cancer. 

Retracted: Yes

PubPeer comments:
 This paper is one of a set of 121 papers that all contain images from the same library of about 100 photos and plots. This is unexpected, because each paper has different authors and different affiliations, with little to no overlap, and describes different cancer types and molecules. It would be very hard to explain how authors from different labs would all obtain the same photo. Therefore, the papers might have all be derived from the same source, such as a paper mill that sells ready-to-be-published papers. This  Stock Photo paper set has been described in more detail on my blog post, https: scienceintegritydigest.com 2020 07 05 the-stock-photo-paper-mill and in a Wall Street Journal article by Eva Xiao (currently behind a paywall): https: www.wsj.com articles chinese-research-papers-raise-doubts-fueling-global-questions-about-scientific-integrity-11593939600 The vast majority (113 121) of the papers were published in the same journal, European Review for Medical and Pharmacological Sciences (ERMPS). A link to the full set of papers can be found here: https: docs.google.com spreadsheets d 172RWS18w1lTNJ9Nnj80fuXXTqTm4fGkoK0sdYyvKE3g edit?usp=sharing All papers have been reported to the journal editors on July 2, 2020. , 
 The publishers have wished the paper into the cornfield. https: www.europeanreview.org article 21864 Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cLong noncoding RNA LUCAT1 promotes migration and invasion of prostate cancer cells by inhibiting KISS1 expression, by C. Liu, L. Wang, Y.-W. Li, Y.-S. Cui, Y.-Q. Wang, S. Liu, published in Eur Rev Med Pharmacol Sci 2019; 23 (8): 3277-3283u2013DOI: 10.26355 eurrev_201904_17689u2013PMID: 31081080u201d has been withdrawn. The Publisher apologizes for any inconvenience this may cause. This does not inspire confidence in the **eight papers** recorded by Google Scholar as having cited the paper. ,