MicroRNA-613 suppresses proliferation, migration and invasion of osteosarcoma by targeting c-MET
Authors: Xufang Sun
Zhihong Li
Xinyu Li
Jing Wu
Journal: American journal of cancer research (2016)
Discipline: CLINICAL MEDICINE
Keywords: c-MET; invasion; miR-613; migration; osteosarcoma
Affiliations: Department of Anesthesiology, The Second Hospital, Jilin University Changchun 130041, China., Daytime Observation Ward, The First Hospital, Jilin University Changchun 130021, China., Department of Thoracic Surgery, The First Hospital, Jilin University Changchun 130021, China.

Abstract:  MicroRNA-613 (miR-613) has been reported to play an important role in the pathogenesis of multiple cancers by negatively regulating gene expression at posttranscriptional level. However, the biological role of miR-613 in osteosarcoma (OS) remained unclear. In this study, we aimed to determine the expression and biological roles of miR-613 in OS. We found that miR-613 was significantly downregulated in OS tissues and cell lines, and that decreased miR-613 expression was correlated negatively with advanced TNM stage and lymph node metastasis. Overexpression of miR-613 in OS cells significantly suppressed the proliferation and colony formation by regulating cell arrest at G0/G1 phase, and impaired the migration and invasive abilities of OS cells, followed by suppression of the epithelial mesenchymal transition (EMT). Bioinformatic and luciferase reporter analysis identified cellular-mesenchymal to epithelial transition factor (c-MET, also named as MET) as a direct target of miR-613. Overexpression of miR-613 significantly inhibited the c-MET expression and its downstream PI3k/Akt/mTOR signaling pathway in OS cells. In OS clinical samples, there was a significant inverse correlation between miR-613 and c-MET mRNA expression. Rescue experiments showed that overexpression of c-MET partially prevented miR-613-induced suppression of OS cell proliferation, colony formation, migration and invasion. In conclusion, we provide first evidence for the suppressive activity of miR-613 by repressing c-MET, suggesting that miR-613 might be a potential therapeutic strategy for OS. 

Retracted: Yes

PubPeer comments:
 Fig 2C.  colony formation ... determined in U2OS cells after transfected with miR-613 mimic or miR-NC. ![file] Fig 2C from [Pang et al (2017)] .  colony formation ...determined in SGC-7901 cells after transfection with miR-138 or miR-NC . ![file] I have concerns. UPDATE: Fig 2C from [Li et al (2017)] . ![file] , 
 *[below]* Fig 2D.  ...cell cycle were determined in U2OS cells after transfected with miR-613 mimic or miR-NC . *[above]* Fig 4A from [Qu et al (2015)] .  Silencing XIAP induced cell arrest and apoptosis in MG63 cells. (A) Cell DNA content distribution in each phase . ![file] Could the authors of either paper comment on the appearance of the same panel in both? , 
 This was retracted...evidently there was  contamination . No DOI available for the Notice but it can be found on PMC : [PMC8263675 ](http: https: www.ncbi.nlm.nih.gov pmc articles PMC8263675 )  Li XY, Sun XF, Wu J, Li ZH. American Journal of Cancer Research. 2016;6:2869-2879. In this article, MicroRNA-613 suppresses proliferation, migration and invasion of osteosarcoma by targeting c-MET we recently found that U2OS cells were contaminated during colony formation experiments due to our careless. Thus, the results of colony formation are not able to reproduce in this study. Therefore, we want to retract this paper in American Journal of Cancer Research. We sincerely apologize for any inconvenience that might result from the retraction of this article. ,