Importance of dimensional changes on glycolytic metabolism during growth
Authors: Neil Armstrong
Claire Thomas
Joffrey Bardin
Sébastien Ratel
Quentin De Larochelambert
Allison Diry
Hugo Maciejewski
Journal: European journal of applied physiology (2020)
Discipline: CLINICAL MEDICINE
Keywords: Accumulated oxygen deficit; Adolescent; Allometric modelling; Lactate; Maturation
Affiliations: French Rowing Federation, 17, boulevard de la Marne, 94130, Nogent-sur-Marne, France., Laboratory Sport, Expertise, and Performance - EA 7370, Research Department, French Institute of Sport (INSEP), Paris, France., AME2P - EA 3533, Clermont-Auvergne University, Clermont-Ferrand, France., Children's Health and Exercise Research Centre, University of Exeter, Exeter, UK., French Institute of Sport (INSEP), IRMES (Institut de Recherche bioMédicale et d'Épidémiologie du Sport) - EA 7329, Paris, France., LBEPS - University of Évry Val d'Essonne, IRBA - Université Paris Saclay, Évry, France., French Rowing Federation, 17, boulevard de la Marne, 94130, Nogent-sur-Marne, France. ., LBEPS - University of Évry Val d'Essonne, IRBA - Université Paris Saclay, Évry, France. .

Abstract:  Purpose: The aim of the present study was to investigate (i) how glycolytic metabolism assessed by accumulated oxygen deficit (AODgly) and blood metabolic responses (lactate and pH) resulting from high-intensity exercise change during growth, and (ii) how lean body mass (LBM) influences AODgly and its relationship with blood markers. Methods: Thirty-six 11- to 17-year olds performed a 60-s all-out test on a rowing ergometer. Allometric modelling was used to investigate the influence of LBM and LBM + maturity offset (MO) on AODgly and its relationship with the extreme post-exercise blood values of lactate ([La]max) and pH (pHmin) obtained during the recovery period. Results: AODgly and [La]max increased while pHmin decreased linearly with LBM and MO (r2 = 0.46 to 0.72, p &lt; 0.001). Moreover, AODgly was positively correlated with [La]max (r2 = 0.75, p &lt; 0.001) and negatively correlated with pHmin (r2 = 0.77, p &lt; 0.001). When AODgly was scaled for LBM, the coefficients of the relationships with blood markers drastically decreased by three to four times ([La]max: r2 = 0.24, p = 0.002; pHmin: r2 = 0.30, p &lt; 0.001). Furthermore, by scaling AODgly for LBM + MO, the correlation coefficients with blood markers became even lower ([La]max: r2 = 0.12, p = 0.037; pHmin: r2 = 0.18, p = 0.009). However, MO-related additional changes accounted much less than LBM for the relationships between AODgly and blood markers. Conclusion: The results challenge previous reports of maturation-related differences in glycolytic energy turnover and suggest that changes in lean body mass are a more powerful influence than maturity status on glycolytic metabolism during growth. 

Retracted: No

PubPeer comments:
 2019 correction. http: www.jbc.org content 294 9 3311.short In Fig. 2E, the images of control and 15(S)-HETE treatments (5 min and 120 min) were duplicated. Similarly, in Fig. 3E, in the control group, the rZO-1 (WT) and rZO-1 (T770A T772A) images were duplicated. These duplications occurred while capturing the images from a group of slides in the dark, and they were amended by replacing with the correct images. This correction does not change the results or conclusions of this work. , 
 Here is an illustration of the duplications as found by ImageTwin. I only marked the  Merged+DAPI panels. * Red and teal boxes:  In Fig. 2E, the images of control and 15(S)-HETE treatments (5 min and 120 min) were duplicated. * Pink boxes:  in Fig. 3E, in the control group, the rZO-1 (WT) and rZO-1 (T770A T772A) images were duplicated. ![file] ,