Long noncoding RNA LINC00511 involves in breast cancer recurrence and radioresistance by regulating STXBP4 expression via miR-185
Authors: A-M Liu
Y Feng
Y Zhu
L Liu
Y Chen
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: LINC00511; breast cancer; radiotherapy; recurrence; survival
Affiliations: Department of Nuclear Medicine, Affiliated Hospital of Southwest Medical University, Luzhou, China. .

Abstract:  Objective: The aim of this study was to explore the molecular function of long intergenic noncoding RNA 00511 (LINC00511) and its target proteins in recurrent breast cancer after breast-conserving surgery followed by radiotherapy. Patients and methods: LINC00511 expression in tissues was measured by quantitative polymerase chain reaction (qPCR). The association between LINC00511 expression and the clinicopathological features of breast cancer was analyzed by Chi-square tests. The impact of LINC00511 on overall survival was evaluated by the log-rank test. MDA-MB-231/MDA-MB-436 cell lines transfected with short hairpin RNA (shRNA) were used to investigate the influence of LINC00511 silencing on tumor growth and radiosensitivity in vitro and in vivo. A series of experiments including cell apoptosis assay, cell colony formation assay, and mouse xenograft models were applied to test those transfected cell lines. MicroRNA (miRNA) targets of LINC00511 were identified by bioinformatics analysis and further validated by dual luciferase reporter assay, qPCR, and Western blot analysis. Results: LINC00511 expression was significantly increased in breast cancer tissues and correlated with recurrence and poor survival after breast-conserving surgery followed by radiotherapy. LINC00511 knockdown by shRNA restricted cell proliferation, promoted cell apoptosis, and enhanced radiosensitivity in vitro, and inhibited tumor growth with an increased response to radiation in vivo. In addition, elevated LINC00511 was found to increase syntaxin-binding protein 4 (STXBP4) expression through competitive binding to miR-185, while silencing LINC00511 decreased STXBP4 expression and increased radiosensitivity. Conclusions: LINC00511 inhibition impairs its competitive binding to miR-185, resulting in increased STXBP4 expression and improved radiation response in breast cancer. Our study results suggest that the LINC00511/miR-185/STXBP4 axis may be a promising therapeutic target for improving the prognosis of breast cancer. 

Retracted: No

PubPeer comments:
 Unexpected similar among all of four images. ![file] , 
 This becomes complicated very quickly but this paper appears to be part of a set of at least 4 papers that appear to share images: * C.-S. Liu , Q. Zhou , Y.-D. Zhang , Y. Fu, Long Noncoding RNA SOX2OT Maintains the Stemness of Pancreatic Cancer Cells by Regulating DEK via Interacting With miR-200a 141, European Review for Medical and Pharmacological Sciences 2020; 24: 2368-2379, DOI: 10.26355 eurrev_202003_20504, https: pubpeer.com publications 22DCA8C70F877D6DA3E7FF32FA36AD#4 * Yong Liu, Chuanping Yang, Chengsong Cao, Qing Li, Xin Jin, Hanping Shi, Hsa_circ_RNA_0011780 Represses the Proliferation and Metastasis of Non-Small Cell Lung Cancer by Decreasing FBXW7 via Targeting miR-544atOncoTargets and Therapy 2020:13 745u2013755, DOI: 10.2147 ott.s236162, https: pubpeer.com publications 16742B0BC40A80589E690FB4E9DB3A * L. Liu , Y. Zhu , A.-M. Liu , Y. Feng , Y. Chen, Long noncoding RNA LINC00511 involves in breast cancer recurrence and radioresistance by regulating STXBP4 expression via miR-185tEuropean Review for Medical and Pharmacological Sciences 2019; 23: 7457-7468, DOI: 10.26355 eurrev_201909_18855, https: pubpeer.com publications 40653AE63E45E7A8FDCA7EFD7E273D * S. Zhao, N.-F. Fan , X.-H. Chen , C.-H. Zhuo , C.-W. Xu , R.-B. Lin, Long noncoding RNA PVT1-214 enhances gastric cancer progression by upregulating TrkC expression in competitively sponging way tEuropean Review for Medical and Pharmacological Sciences 2019; 23: 4173-4184, DOI: 10.26355 eurrev_201905_17920, https: pubpeer.com publications 90826D8F19D103A269F03F36B02D19 There does not appear to be an overlap between the authors of these papers, suggesting these papers might all contain outsourced photos generated by the same source. The authors are encouraged to take away any concern by sharing original data. Here are the colony formation photos from 3 of these papers, with colored circles marking photos that look remarkably similar, albeit sometimes rotated or with specific areas altered. Based on the light reflection in the wells, I will call this the  Golden Arches paper set . ![file] ![file] ![file] , 
 Three of the four papers in the Golden Arches set also appear to share tumor photos, although they represent mice injected with different types of cancer cell line. ![file] ,