Retracted : MicroRNA‐184 inhibits proliferation and promotes apoptosis of human colon cancer SW480 and HCT116 cells by downregulating C‐MYC and BCL‐2
Authors: Gang Li
Wei Qiu
Xiao-Hui Zhao
Yong-Bing Wang
Jun-Hua Zheng
Journal: Journal of Cellular Biochemistry (2018)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: BCL-2; C-MYC; MicroRNA-184; apoptosis; colon cancer
Affiliations: Department of General Surgery, Shanghai Pudong New Area People's Hospital, Shanghai, China., Department of Neurology, Shanghai Pudong New Area People's Hospital, Shanghai, China., Department of Proctology, Shanghai Pudong New Area People's Hospital, Shanghai, China.

Abstract:  This study aimed to investigate the effects of microRNA-184 (miR-184) on the proliferation and apoptosis of human colon cancer cells through the regulation of C-MYC and BCL-2. Human colon cancer tissues were selected as case group, and adjacent normal tissues were as control group. Human colon cancer SW480 and HCT116 cells were allocated into blank, miR-184 mimic negative control (mimic-NC), miR-184 inhibitor NC (inhibitor-NC), miR-184 mimic, and miR-184 inhibitor groups. Flow cytometry, Annexin V/PI and MTT assay were used to examine the cell cycle, apoptosis and viability. The expressions of C-MYC, BCL-2 and miR-184 were detected via immunohistochemistry, Western blotting and reverse transcription quantitative polymerase chain reaction (RT-qPCR). C-MYC and BCL-2 were direct targets to miR-184. The growth of colon cancer cells in the miR-184 mimic group was inhibited and exhibited an increase in apoptosis. Cell growth in the miR-184 mimic group was increased in addition to the inhibition of apoptosis. Compared with miR-184 mimic group, the expressions of C-MYC and BCL-2 in miR-184 inhibitor group were increased. The expressions of C-MYC and BCL-2 in colon cancer tissues exhibited high levels of expression, while miR-184 displayed relatively low levels in comparison to the adjacent normal tissues. An association was detected regarding the expressions of miR-184, C-MYC and BCL-2 with the differentiation, invasion depth and lymph node metastasis. MiR-184 expression was negatively related to C-MYC and BCL-2 expressions. Our study suggested that miR-184 could inhibit proliferation and promote apoptosis of colon cancer cells by down-regulating expressions of C-MYC and BCL-2. 

Retracted: Yes

PubPeer comments:
 Figure 6 A and C blots appear to have the same bands for Western blots of different cell lines. ![file] , 
 #1 From left to right here: Fig 5B and 4B from [Wen et al (2018)] . Then 6E from [Zhang et al (2018)] . ![] Repeated control bands, which also have similarities to the control bands of the present 6A C. ![file] , 
 Fig 4A,C.  Cell cycles of SW480 and HCT116 cells among four groups at 48 h after transfection by flow cytometry with PI staining Notes: A-B, cell cycle of SW480 cell among four groups; C-D, cell cycle of HCT116 cell among four groups ![file] I am not convinced that the raw cell-count histograms should exhibit such alarming overhangs. Nor am I convinced that some of the results for SW480 and HCT116 should be so similar. , 
 Fig 4A again: ![file] Fig 8A from [Jin et al (2018)] :  Flow cytometry analysis of PI staining shows that SUMO2 gene silencing promotes cell cycle entry of NPCs Notes: (A) Cell cycle entry of NPCs in the sham, model, NC, and shRNA groups . ![file] Areas of similarity marked. , 
 Retracted [19 July 2021] . ---------------------------------------------- The above article, published online on 07 August 2017 in Wiley Online Library has been retracted by agreement between the journal s Editor in Chief, Prof. Dr. Christian Behl, and Wiley Periodicals LLC. The retraction has been agreed following an investigation based on allegations raised by a third party. A detailed investigation revealed that several image elements of the experimental data were published elsewhere in a different scientific context. Thus, the editors consider the conclusions of this article to be invalid. ,