Natural Killer Cell-Derived Exosomal miR-3607-3p Inhibits Pancreatic Cancer Progression by Targeting IL-26
Authors: Shengjie Dai
Wen Ye
Kai Qi
Qiye He
Jie Zhang
Hongwei Sun
Hongru Kong
Tuo Deng
Mengtao Zhou
Keqing Shi
Journal: Frontiers in Immunology (2019)
Discipline: IMMUNOLOGY
Keywords: IL-26; extracellular vesicles; miR-3607-3p; natural killer; pancreatic cancer.
Affiliations: Department of Hepatobiliary Surgery, Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of ZheJiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China., Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of ZheJiang Province, Center of Precision Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China., Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences, Shanghai, China., Singlera Genomics Inc., San Diego, CA, United States., Singlera Genomics (Shanghai) Ltd., Shanghai, China., Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of ZheJiang Province, Precision Medical Center Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Abstract:  Increasing evidences have suggested that natural killer (NK) cells in the tumor microenvironment are involved in the regulation of cancer development. However, the potential biological roles and regulatory mechanisms of NK cells in pancreatic cancer (PC) remain unclear. Co-culture system of NK cells with PC cells is used to test the ability of cancer cell proliferation, migration and invasion both in vitro and in vivo. And tail vein intravenous transfer was used to test metastasis in vivo. Meanwhile, extracellular vesicles (EVs) were separated and examined. Furthermore, reporter assay and Biotin-RNA pull down assay were performed to verify the interaction between molecules. NK cells can inhibit the malignant transformation of co-cultured PC cells both in vivo and in vitro, which requires miR-3607-3p. miR-3607-3p is found enriched in the EVs of NK cells and transmitted to PC cells, and low level of miR-3607-3p predicts poor prognosis in PC patients. It can also inhibit proliferation, migration and invasion of PC cells in vitro. Importantly, IL-26 is found to be a direct target of miR-3607-3p in PC cells. miR-3607-3p enriched in EVs derived from NK cells can inhibit the malignant transformation of PC probably through directly targeting of IL-26. 

Retracted: No

PubPeer comments:
 u201cIn the original article, there was a mistake in Figure 1 and Figure 5 as published. Figures 1E and Figure 5F were **wrongly placed**. The corrected Figure 1 and Figure 5 appears below. The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.u201d (emphasis added) The u201cwrongly placedu201d image 1E shows below. I would have described the error differently. *** ![file] , 
 Oopsies. Another u201cwrongly placedu201d image may be found in Figure 5E as well, although my description for this tends to be u201cimage overlap.u201d Iu2019ve seen this type of error in papermill publications before. Note: The corresponding authoru2018s email address thereu2019s no resemblance to any author s name. *** ![file] ,