Dll4/Notch1 signalling pathway is required in collective invasion of salivary adenoid cystic carcinoma
Authors: Hua-Yang Fan
Ke Wang
Jian Jiang
Ya-Ling Tang
Mei Zhang
Xiang-Hua Yu
Jia-Shun Wu
Bing-Jun Chen
Xin-Hua Liang
Xing Pang
Journal: Oncology Reports (2021)
Discipline: CLINICAL MEDICINE
Keywords: salivary adenoid cystic carcinoma; δ-like ligand 4; Notch1; collective invasion; migration and invasion.
Affiliations: State Key Laboratory of Oral Diseases, Department of Oral and Maxillofacial Surgery, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China., Department of Head and Neck Surgery, Sichuan Cancer Institute, Sichuan Cancer Center, Sichuan Cancer Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan 610000, P.R. China., State Key Laboratory of Oral Diseases, Department of Oral Pathology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China.

Abstract:  High expression of δ‑like ligand 4 (Dll4) is reportedly related to the invasion, metastasis, and clinical prognosis of various malignant tumours. Our previous study revealed that collective cell invasion was a common pattern in salivary adenoid cystic carcinoma (SACC). However, the roles of the Dll4/Notch1 signalling pathway in the collective invasion of SACC remain unclear. The present study revealed that Dll4 expression was higher at the invasive front of SACC, and that this upregulation was associated with solid tumour type, high TNM grade, and high rates of metastasis and recurrence. Furthermore, the expression levels of Notch1 and Dll4 were positively correlated at the invasive front, and a three‑dimensional (3D) culture model revealed that leader cells showed high expression of Dll4, while follower cells showed high expression of Notch1. Moreover, silencing of Dll4 expression using small interfering RNA reduced the migration, invasion, and collective invasion of SACC cells, and these abilities were rescued by Notch1 overexpression. Finally, SACC collective invasion was increased via the Dll4/Notch1 signalling pathway in experiments that involved a stiff 3D gel, hypoxia and co‑culture with human endothelial cells. These findings indicated that the Dll4/Notch1 signalling pathway may be involved in the collective invasion of SACC, which may help to provide possible targets for the treatment of SACC. 

Retracted: No

PubPeer comments:
 Figure 6B appears to have a repeated image. *** ![file] , 
 Thanks for your information! Our group paid highly attention to this comment! We had carefully checked the figure and found that we have made a mistake during pasting Figure 6B image. So we will contact the editor as soon as possible to replace the repeated image. Thanks again for your comment and we will pay attention to this and will not make the same mistakes in the future. , 
 #2 Is there an update on this? Authors with a same or similar name appear to have multiple papers with similar issues: https: pubpeer.com search?q=authors%3A%22Ya-ling+Tang%22 , 
 Thanks for your attention! We have contacted Journal and asked for a corrigendum of our article entitled  Dll4 Notch1 signal pathway is required in collective invasion of salivary adenoid cystic carcinoma (DOI: 10.3892 or.2021.7939; Manuscript No: 262723). And the Journal has been dealing with this after we showed the proof of ID! We will contact again. In addition, we have contacted the Journals of the other papers with similar issues and step by step we will do all . Please give us some times to make a corrigendum for them. The following has been done. Corrigendum: Overexpression Cathepsin D contributes to perineural invasion of salivary adenoid cystic carcinoma In the future we will pay more attention to this! Thanks! , 
 Retraction of a paper with overlapping authors: https: pubpeer.com publications EC4B69E153BE5814CC21E40C13A3CE , 
 Corrigendum, [19 January 2022] .  Following the publication of this article, the authors have realized that they made an error during the compilation of the images shown in Fig. 6, and that this error was not corrected before the paper was sent to press. Specifically, in Fig. 6B, the data panels showing the results from the HUVEC + SACCu201183 siu2011Dll4 and HUVEC + SACCu2011LM siu2011Dll4 experiments at 24 h were inadvertently repeated. The corrected version of Fig. 6, showing the correctly assembled data panels for Fig. 6B, is shown on the next page. The authors sincerely apologize for the errors that were introduced during the preparation of this Figure, thank the Editor for allowing them the opportunity to publish this Corrigendum, and regret any inconvenience that these errors may have caused. ,