CircRNA UBAP2 promotes the progression of ovarian cancer by sponging microRNA-144
Authors: M Sheng
M Yan
Q-X Zhao
N Wei
H-Y Yang
L-J Jing
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: Circular RNA UBAP2; circUBAP2; ovarian cancer; microRNA-144; CHD2
Affiliations: Department of Obstetrics, Weifang Yidu Center Hospital, Weifang, China. .

Abstract:  Objective: This study aims to elucidate the regulatory effect of circular RNA UBAP2 (circUBAP2) on the progression of ovarian cancer (OC). Patients and methods: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detect the expressions of circUBAP2, microRNA-144 and CHD2 in OC tissues and adjacent normal tissues. The correlation between the expression levels of circUBAP2 and microRNA-144 with pathological parameters of OC patients was analyzed. Subcellular distribution of circUBAP2 was detected by chromatin fractionation assay. After overexpression of circUBAP2 in OC cells, changes in proliferative and migratory abilities were evaluated by Cell Counting Kit-8 (CCK-8) and transwell assay, respectively. In addition, the Dual-Luciferase reporter gene assay was used to verify the binding of circUBAP2 and microRNA-144, and the binding of CHD2 to microRNA-144. Results: QRT-PCR results showed that circUBAP2 was highly expressed in OC tissues, and its expression was negatively correlated with TMN stage and five-year survival of OC patients. CircUBAP2 was mainly distributed in the cytoplasm. Overexpression of circUBAP2 significantly promoted the proliferative and migratory abilities of OC cells. The Dual-Luciferase reporter gene assay demonstrated that circUBAP2 could bind to microRNA-144. Meanwhile, circUBAP2 negatively regulated microRNA-144 expression in OC cells. Besides, the promotive effects of circUBAP2 on the proliferation and migration of OC cells were reversed by microRNA-144 overexpression. MicroRNA-144 was lowly expressed in OC tissues, which was negatively correlated with TNM stage of OC patients. The Dual-Luciferase reporter gene assay confirmed the binding condition between CHD2 and microRNA-144. CHD2 expression was negatively regulated by microRNA-144 in OC cells. Moreover, CHD2 could bind to microRNA-144 and partially inhibited its activity, thereby promoting the proliferative and migratory abilities of OC cells. Conclusions: CircUBAP2 promotes the progression of ovarian cancer by adsorbing microRNA-144. 

Retracted: No

PubPeer comments:
 This article has been retracted:  The article titled u201cDownregulation of ADAM10 Expression Inhibits Metastasis and Invasiveness of Human Hepatocellular Carcinoma HepG2 Cellsu201d [1] has been retracted as it is found to contain a substantial amount of material, without referencing, from the paper u201cADAM10 is overexpressed in human hepatocellular carcinoma and contributes to the proliferation, invasion and migration of HepG2 cells,u201d by Shao Yuan, Shi Lei, and Shengli Wu published in Oncology Reports, vol. 2013, 1715-1722 pages, DOI: 10.3892 or.2013.2650. [That paper ](http: doi.org 10.3892 or.2013.2650) shares some of the same authors as this paper. It also shares some figures: Yuan Yue 2013 figure 1 ![file] Shao Yuan 2013 figure 6: ![file] Yuan Yue figure 2: ![file] Shao Yuan figure 8: ![file] It also shares these figures with a [different paper](http: doi.org 10.3892 or.2014.3503) with no common authors and different affiliations: ![file] This paper was published a year after the previous papers. , 
 So the images appear in -  [Downregulation of ADAM10 expression inhibits metastasis and invasiveness of human hepatocellular carcinoma HepG2 cells]  (Yue et al 2013): retracted from BioMed Research International. -  [ADAM10 is overexpressed in human hepatocellular carcinoma and contributes to the proliferation, invasion and migration of HepG2 cells]  (Yuan et al 2013): cited as the reason for that retraction. -  [Synergistic effects of co-expression plasmidu2011based ADAM10-specific siRNA and GRIM-19 on hepatocellular carcinoma in vitro and in vivo]  (Liu et al 2014). Have I missed anything? ,