MiR-155 affects osteosarcoma cell proliferation and invasion through regulating NF-κB signaling pathway
Authors: L Tang
Q-S Liao
S Lu
Journal: European review for medical and pharmacological sciences (2018)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: Osteosarcoma; miR-155; cell proliferation; apoptosis; NF-κB signaling pathway
Affiliations: College of Optoelectronic Engineering, Health Science Center Shenzhen University, Shenzhen, Guangdong, China. .

Abstract:  Objective: Osteosarcoma can form tumor osteoid tissue and bone tissue directly or indirectly through cartilage stage. It mainly occurs in children and adolescents with high mortality. MicroRNA (miRNA) possesses tissue sensitivity as tumor biomarker and plays a promoting or inhibitory role in tumor pathogenesis as oncogene or tumor suppressor gene. It was found that miR-155 was abnormally expressed in tumor and could be treated as a biomarker for cancer progression. However, miR-155 expression in osteosarcoma and related mechanism still remains unclear. This study aimed to explore the role of miR-155 in osteosarcoma occurrence and development. Materials and methods: Osteosarcoma cell line MG-63 was cultured in vitro and transfected by miR-155 mimics or inhibitor. MiR-155 expression was examined by Real-time PCR (RT-PCR). Cell proliferation was evaluated by MTT assay. Caspase 3 activity was determined by caspase 3 activity detection kit. Cell invasion was measured by transwell assay. B-cell lymphoma-2 (Bcl-2) and nuclear factor κB (NF-κB) protein expressions were assessed by Western blot. Results: MiR-155 mimics significantly up-regulated miR-155 expression, promoted MG-63 cell proliferation and invasion, inhibited caspase 3 activity, and up-regulated expressions of NF-κB and Bcl-2 compared with control group (p &lt; 0.05). However, miR-155 inhibitor significantly inhibited MG-63 cell proliferation and invasion, enhanced caspase 3 activity, and reduced expressions of NF-κB and Bcl-2 compared with control group (p &lt; 0.05). Conclusions: MiR-155 affected osteosarcoma cell proliferation and apoptosis through regulating NF-κB signaling pathway, indicating it might be a new biomarker for osteosarcoma diagnosis and treatment. 

Retracted: No

PubPeer comments:
 This is a very puzzling paper. As claimed in the abstract, the authors intended to proposed a general class of u201c**distance measures**.u201d However, the u201cdistance measureu201d presented in this paper may not be a distance measure in the usual sense. For instance, the measure in eq.(15) cannot be a **distance measure** because it does not meet the triangle inequality. For example, let $$a= (1, 2, 3)$$ $$b= (1, 3, 2)$$ $$c= (3, 1, 2)$$ According to eq.(15), $$MD_2(a, c) =1$$ $$MD_2(a, b) =MD_2(b,c)=0$$ and $$ MD_2(a, c) MD_2(a, b) + MD_2(b,c)$$ ![file] ![file] , 
 Whatu2019s you commented just shows your ignorance! In fact, your puzzliness has been detailedly replied in the following link: [https: pubpeer.com publications BEE49F054B9FBBB3F8F8F6E067E807]. It says u201cIn your u201ceyesu201d, all are not u201cnewu201d, all are u201cmeaninglessu201d, u201cpuzzlingu201d, and u201cnot soundu201d! What a pessimistic and depressing person! Absurdly comment dozens of papers every day and it is said that you have commented thousands of papers on Pubpeer recently! Who do you think you are? Criticizing and raving almost all the experts and the papers in the decision making field! Just a man with madness!u201d It wastes time to discuss here with an idiot and madman! ,