Effects of miR-223 on colorectal cancer cell proliferation and apoptosis through regulating FoxO3a/BIM
Authors: Z-B Tian
J-Y Tan
B Cao
M-Q Song
H Ju
Journal: European review for medical and pharmacological sciences (2018)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: Colorectal cancer; Bcl-2 interacting mediator of cell death (BIM); FoxO3a; microRNA-223 (miR-223); cell proliferation
Affiliations: Department of Gastroenterology, Affiliated Hospital Of Qingdao University, Qingdao, Shandong, China. .

Abstract:  Objective: Colorectal cancer is a common malignant tumor of the digestive tract. It frequently occurs at the junction of the rectum and sigmoid colon. It is characterized by high mortality and poor prognosis. Bcl-2 interacting mediator of cell death (BIM) plays a role in the regulation of cell proliferation and apoptosis, and involves in the pathogenesis of colorectal cancer. The transcription factor forkhead, transcription factor O subfamily 3a (FoxO3a) plays a role in the regulation of BIM expression and is associated to the pathogenesis of colorectal cancer. Bioinformatics analysis suggests that there is a targeted relationship between FoxO3a and microRNA-223 (miR-223). This study aims to investigate effects of miR-223 on the regulation of FoxO3a/BIM signaling pathway and colorectal cancer cell proliferation and apoptosis. Materials and methods: Colorectal cancer cell line SW620 and normal colorectal epithelial cell line NCM460 were cultured in vitro. Dual luciferase reporter assay was used to validate the relationship between miR-223 and FoxO3a. Flow cytometry was adopted to detect apoptosis. EdU staining was applied to test cell proliferation. Western blot was selected to determine FoxO3a and BIM protein expressions. Results: There was targeted regulatory relationship between miR-223 and FoxO3a. MiRa-223 up-regulated, FoxO3a and BIM expressions reduced, and cell proliferation was enhanced in SW620 cells compared with NCM460 cells. MiR-223 inhibitor or pIRES2-FoxO3a transfection significantly increased FoxO3a and BIM expressions, attenuated cell proliferation, and enhanced cell apoptosis. Conclusions: MiR-223 targeted inhibited expression of FoxO3. Down-regulating the expression of miR-223, it increased the expressions of FoxO3a and BIM, weakened SW620 cells proliferation and induced apoptosis. 

Retracted: No

PubPeer comments:
 This paper has features suggestive of [what has elsewhere been labeled as  Papermill #2 ] , characterised by stylised geometrical WBs that are reduced to black blobs on a featureless grey background, reminiscent of a Miro painting, though in this example the background is more varied and the edges are blurrier than usual: ![file] The other characteristic is a small repertoire of recurring flow cytometry plots, ![file] slightly modified by artisanal stippling on each of their occurrences. ![] Other examples are listed in a Google document. https: docs.google.com spreadsheets d 1qBqQMXgzxoBd5bTbqdMuapGheupE5bdIopypju417NM edit?usp=sharing The connection between the name of the nominal corresponding author and the corresponding email address is not obvious. ![file] , 
 #1 I think the email weibachengyuan@163.com may belong to the corresponding author. The authors were from Department of Gastroenterology,  wei means Gastric(u80c3),  ba means eight(u516b), and  chengyuan (u6210u5458) means group member(s). So the mean of prefix is member of the eighth group of gastroenterology(u80c3u516bu6210u5458). This will cause confusion to people who don t use Chinese, and it makes people wonder whether the corresponding author had actually logged in to this email. , 
 http: www.most.gov.cn zxgz kycxjs kycxgzdt 202201 t20220127_179206.html Google Translated Date: January 27, 2022 14:44 Source: Ministry of Science and Technology u3000u30007. The paper  Effects of miR-223 on colorectal cancer cell proliferation and apoptosis through regulating FoxO3a BIM by Ju Hui from Qingdao University Affiliated Hospital as the corresponding author and the first author. After investigation, the paper was found to be fraudulent. Ju Hui will be dealt with as follows: demerit for 1 year, cancel application or application for science and technology projects (special projects, funds, etc.), science and technology awards, and qualifications for science and technology talent titles for 5 years, cancel as nominee or recommender, nominated or recommender, reviewer Experts and other qualifications for 5 years will be recorded in the archives of scientific research integrity and dishonesty, given a lecture on scientific research integrity, and will be canceled from participating in this year s evaluation. u3000u3000(Notification link: https: kjc.qdu.edu.cn info 1023 3176.htm) ,