MiR-203 regulates DJ-1 expression and affects proliferation, apoptosis and DDP resistance of pancreatic cancer cells
Authors: X-L Cheng
L-Y Xu
Q-S Liu
Z-H Dong
Z-H Mo
Z-Z Fan
F-F Lu
S-L Du
P Gao
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: DJ-1; miR-203; pancreatic cancer; tumorigenesis; PTEN
Affiliations: Department of Medical Imaging, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan, China. .

Abstract:  Objective: DJ-1 is a negative regulator of PTEN and plays a role in tumorigenesis. Abnormal miR-203 expression is associated with pancreatic cancer. Bioinformatics analysis showed a targeted relationship between miR-203 and DJ-1 3'-UTR. This study investigated whether miR-203 regulates DJ-1 expression and its role in pancreatic cancer cell proliferation, apoptosis, and cisplatin (DDP) resistance. Materials and methods: The Dual-Luciferase reporter gene assay validated the targeted regulation between miR-203 and DJ-1. The DDP-resistant cell line SW1990/DDP was established and divided into miR-NC group and miR-203 mimic group followed by analysis of the expression of DJ-1, PTEN and p-AKT, cell apoptosis, and proliferation. Results: There was a targeted relationship between miR-203 and DJ-1 mRNA. The expression of miR-203 in SW1990/DDP cells was significantly lower than that in SW1990 cells, while the expression of DJ-1 mRNA and protein was significantly higher than that in SW1990 cells. Compared with miR-NC group, the expression of DJ-1 and p-AKT protein in SW1990/DDP cells was significantly decreased in miR-203 mimic transfection group, while the expression of PTEN was significantly increased with increased cell apoptosis and decreased cell proliferation, as well as reduced DDP resistance. Conclusions: The decreased expression of miR-203 and the increased expression of DJ-1 is associated with drug resistance in pancreatic cancer cells. Elevated miR-203 can inhibit the expression of DJ-1, affect the activity of PTEN-PI3K/AKT pathway, inhibit the proliferation of pancreatic cancer cells, induce cell apoptosis, and reduce DDP resistance of pancreatic cancer cells. 

Retracted: No

PubPeer comments:
 Please appreciate the effort I put into creating this Gallery of Unfortunate FACS Plots. Fig 4D [Li et al (2019)] 10.26355 eurrev_201909_18832 Fig 5D [Liu et al (2019)] 10.26355 eurrev_201911_19421 Fig 4E [Du et al (2019)] 10.26355 eurrev_201910_19278 Fig 4D [Dong et al (2019)] 10.26355 eurrev_201907_18305 ![file] Fig 4E [Wu et al (2019)] 10.26355 eurrev_201909_18852 Fig 3C [Cao et al (2019)] 10.26355 eurrev_201908_18531 Fig 4E [Wang et al (2019)] 10.26355 eurrev_201906_18181 Fig 4C [Wei et al (2019)] 10.26355 eurrev_201907_18299 ![file] , 
 #1 These eight papers also display a strange stylised form of Western Blots, where out-of-focus geometrical ellipses hover above a featureless background. Shadowy wisps of smoke hang in the foreground but there is no real-world detail. Did I mention the absence of overlap among the different teams of authors, and their far-flung locations? Figs 3C, 4C [Li et al (2019)] 10.26355 eurrev_201909_18832 Figs 4B, 5B [Liu et al (2019)] 10.26355 eurrev_201911_19421 Figs 3C, 4C [Du et al (2019)] 10.26355 eurrev_201910_19278 Figs 3C, 4C [Dong et al (2019)] 10.26355 eurrev_201907_18305 ![file] Figs 2B, 4C [Wu et al (2019)] 10.26355 eurrev_201909_18852 Figs 2B, 3B [Cao et al (2019)] 10.26355 eurrev_201908_18531 Figs 3B, 4C [Wang et al (2019)] 10.26355 eurrev_201906_18181 Figs 3B, 4B [Wei et al (2019)] 10.26355 eurrev_201907_18299 ![file] ,