MicroRNA-10b promotes migration and invasion through KLF4 and HOXD10 in human bladder cancer
Authors: Hua Xu
Jin Zeng
Junhui Hu
Wei He
Heng Li
Haibing Xiao
Gan Yu
Zhangqun Ye
Wei Xiao
Guohua Zeng
Kun Tang
Journal: Oncology reports (2014)
Discipline: CLINICAL MEDICINE
Keywords: miR-10b; cell migration; invasion; bladder cancer; KLF4
Affiliations: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, P.R. China., Translational Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, P.R. China., Department of Urology, Minimally Invasive Surgery Center, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, P.R. China.

Abstract:  The present study was performed to investigate the effect of microRNA-10b (miR-10b) on cell migration and invasion in human bladder cancer (BC). Real-time PCR was performed to detect the expression of miR-10b in BC cell lines. miR-10b mimics, the negative control for mimics, miR-10b inhibitor and the negative control for inhibitor were transfected into BC cell lines and the effects of miR-10b on the migration and invasion of cells were investigated through Transwell assay. Meanwhile, protein levels of KLF4, HOXD10, E-cadherin and MMP14 were measured. Luciferase assays were also performed to validate KLF4 and HOXD10 as miR-10b targets. In vivo metastasis assay was performed to validate if miR-10b can promote BC cell line metastasis in vivo. miR-10b is significantly upregulated in BC cell lines and metastatic tissues. Increased miR-10b expression significantly enhanced BC cell migration and invasion, while decreased miR-10b expression reduced cell migration and invasion. In vivo metastasis assay demonstrated that overexpression of miR-10b markedly promoted BC metastasis. Moreover, KLF4 and HOXD10 were identified as direct targets of miR-10b in BC cells. Silencing of KLF4 or HOXD10 recapitulated the pro-metastatic function. Furthermore, we found that E-cadherin and MMP14 may be the downstream factors of KLF4 and HOXD10 in the suppression of BC metastasis by miR-10b. These data suggest that miR-10b may function as oncogenes in BC cells. Targeting these novel strategies, inhibition of miR-10b/KLF4/E-cadherin axis and miR-10b/HOXD10/MMP14 axis may be helpful as a therapeutic approach to block BC cell metastasis. 

Retracted: No

PubPeer comments:
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 The article examined the influence of attention, relatedness, and valence of emotional pictures on memory. The results replicate some well-established main effects. 1. Divided attention reduces memory performance. 2. Related pictures are recalled better. 3. Emotional arousing stimuli are remembered better than neutral pictures. 4. The effect of arousal on memory is at least partially mediated by attention. The article also produced some novel findings that suggested negative stimuli are remembered better independent of the other factors. However, a z-curve analysis (see attached picture) shows that these new results had low power. The results have also not been replicated. Therefore, readers should be careful in interpreting these results and the article should not be cited as evidence that negative stimuli have a memory advantage. ![file] ,