MiR-203 regulates proliferation and apoptosis of ovarian cancer cells by targeting SOCS3
Authors: H-P Liu
X-M Zheng
Y Zhang
H Qi
L-H Qi
Z-T Liu
J-Y Wang
Journal: European Review for Medical and Pharmacological Sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: SOCS3; miR-203; ovarian cancer; JAK-STAT pathway; proliferation
Affiliations: Department of Reproductive Medicine Center, The 960th Hospital of the PLA Joint Logistics Support Force, Jinan, Shandong, China. .

Abstract:  Objective: Cytokine signal transduction inhibitor 3 (SOCS3) negatively regulates Janus kinases (JAK) - signal transducer and activator of transcription (STAT) pathway. Bioinformatics analysis revealed a targeted relationship between miR-203 and SOCS3 mRNA. This study investigated the role of miR-203 in ovarian cancer cell proliferation and apoptosis. Patients and methods: Ovarian cancer tissues and adjacent tissues were collected to detect the expression of miR-203 and SOCS3. Ovarian cancer HO8910 cells were divided into miR-NC group, miR-203 inhibitor group, and miR-203 mimic group followed by the analysis of the expression of miR-203 and SOCS3 mRNA by quantitative Reverse Transcription-PCR (qRT-PCR), protein expression of p-JAK2 and p-STAT3 by Western blot, cell apoptosis by flow cytometry, and proliferation by 5-Ethynyl-2'-deoxyuridine (EdU) staining chronologically. Results: Compared with adjacent tissues, miR-203 expression was significantly increased in tumor tissues and SCOS3 mRNA expression was decreased. Compared with those with lower miR-203 expression, the prognosis of patients with higher expression of miR-203 was significantly worse. There was a targeted regulatory relationship between miR-203 and SOCS3 mRNA. Compared with IOSE80 cells, miR-203 expression in HO8910 and SKOV3 cells was increased, and its expressions of SOCS3 mRNA and protein were decreased. Compared with miR-NC group, the transfection of miR-203 inhibitor significantly increased SOCS3 expression, and decreased the expression of p-JAK2 and p-STAT3 protein. We draw the conclusion that miR-203 increased cell apoptosis and decreased cell proliferation. However, opposite results were observed after the transfection of miR-203 mimic. Conclusions: Abnormal miR-203 and SOCS3 expression are related to the pathogenesis of ovarian cancer. MiR-203 affects the proliferation of JAK-STAT pathway and regulates the proliferation and apoptosis of ovarian cancer cells by targeting the inhibition of SOCS3 expression. 

Retracted: Yes

PubPeer comments:
 Please appreciate the effort I put into creating this Gallery of Unfortunate FACS Plots. Fig 4D [Li et al (2019)] 10.26355 eurrev_201909_18832 Fig 5D [Liu et al (2019)] 10.26355 eurrev_201911_19421 Fig 4E [Du et al (2019)] 10.26355 eurrev_201910_19278 Fig 4D [Dong et al (2019)] 10.26355 eurrev_201907_18305 ![file] Fig 4E [Wu et al (2019)] 10.26355 eurrev_201909_18852 Fig 3C [Cao et al (2019)] 10.26355 eurrev_201908_18531 Fig 4E [Wang et al (2019)] 10.26355 eurrev_201906_18181 Fig 4C [Wei et al (2019)] 10.26355 eurrev_201907_18299 ![file] , 
 #1 These eight papers also display a strange stylised form of Western Blots, where out-of-focus geometrical ellipses hover above a featureless background. Shadowy wisps of smoke hang in the foreground but there is no real-world detail. Did I mention the absence of overlap among the different teams of authors, and their far-flung locations? Figs 3C, 4C [Li et al (2019)] 10.26355 eurrev_201909_18832 Figs 4B, 5B [Liu et al (2019)] 10.26355 eurrev_201911_19421 Figs 3C, 4C [Du et al (2019)] 10.26355 eurrev_201910_19278 Figs 3C, 4C [Dong et al (2019)] 10.26355 eurrev_201907_18305 ![file] Figs 2B, 4C [Wu et al (2019)] 10.26355 eurrev_201909_18852 Figs 2B, 3B [Cao et al (2019)] 10.26355 eurrev_201908_18531 Figs 3B, 4C [Wang et al (2019)] 10.26355 eurrev_201906_18181 Figs 3B, 4B [Wei et al (2019)] 10.26355 eurrev_201907_18299 ![file] , 
 Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cMiR-203 regulates proliferation and apoptosis of ovarian cancer cells by targeting SOCS3, by H.-P. Liu, Y. Zhang, Z.-T. Liu, H. Qi, X.-M. Zheng, L.-H. Qi, J.-Y. Wang, published in Eur Rev Med Pharmacol Sci 2019; 23 (21): 9286-9294u2013DOI: 10.26355 eurrev_201911_19421u2013PMID: 31773696u201d has been withdrawn. [The Publisher apologizes for any inconvenience this may cause] . ![file] ![file] Really? ,