Retracted : Long noncoding RNA CHRF exacerbates IL‐6‐induced inflammatory damages by downregulating microRNA‐146a in ATDC5 cells
Authors: Chuandong Yu
Zhilin Li
Xuefeng Shi
Guang Wan
Donglei Shi
Journal: Journal of Cellular Physiology (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: NF-κB/JAK1/STAT3; inflammatory damages; lncRNA CHRF; miR-146a; osteoarthritis.
Affiliations: Department of Orthopedics Heze Municipal Hospital Heze China, Department of Orthopedics The First Affiliated Hospital of Xinxiang Medical University Weihui China, Department of Orthopedics Trauma &amp; Hand and Foot Surgery, Jinan Central Hospital Affiliated to Shandong University Jinan China

Abstract:  Osteoarthritis (OA) is a frequent chronic musculoskeletal disorder which lacks applicably effective therapeutic strategy. In this study, we attempted to investigate whether long noncoding RNA (lncRNA) cardiac hypertrophy-related factor (CHRF) participated in mediating interleukin-6 (IL-6)-induced in vitro inflammatory damages as well as the regulatory mechanisms. ATDC5 cells were stimulated with IL-6, and then cellular damages were evaluated on the basis of cell viability by CCK-8, apoptotic cells by observation with flow cytometry, apoptosis-associated proteins by western blot analysis, and accumulation of inflammatory factors by quantitative reverse transcription polymerase chain reaction (RT-qPCR), enzyme-linked immunosorbent assay (ELISA), and western blot analysis. Then, effects of lncRNA CHRF on IL-6-treated cells were evaluated. We further explored the downstream factor of lncRNA CHRF and demonstrated whether lncRNA CHRF functioned through the downstream factor. Afterwards, crucial signaling cascades were anatomized. We found that IL-6 reduced cell viability, elevated apoptosis, induced upregulation of inflammatory factors, as well as upregulated lncRNA CHRF and down-regulated miR-146a expression. Then, we found lncRNA CHRF overexpression aggravated IL-6-induced alterations, and lncRNA CHRF knockdown showed the opposite effects. Furthermore, miR-146a was identified to be negatively regulated by lncRNA CHRF, and its overexpression abrogated the roles of lncRNA CHRF in IL-6-treated cells. IL-6-induced the accumulation of IκBα, p65, JAK1, and STAT3 at phosphorylated level was further facilitated by lncRNA CHRF whereas repressed by miR-146a. In conclusion, lncRNA CHRF aggravated the IL-6-induced inflammatory damages in ATDC5 cells. We further outlined a possible mechanism that through downregulating miR-146a, lncRNA CHRF evoked the activation of NF-κB and JAK1/STAT3 signaling cascades. 

Retracted: Yes

PubPeer comments:
 Brought to my attention by a reader. * Red boxes: Two lanes in the GAPDH panel in Figure 1D look similar to two lanes in the GAPDH panel in Figure 3B but they represent different conditions * Blue boxes: Two lanes in the left p62 panel in Figure 1D look similar, if flipped horizontally, to the right Cyclin B lanes in Figure 3B. ![file] , 
 Thank you for your inquiry. We will seriously investigate and deal with it as soon as possible. , 
 2020 retraction. https: www.spandidos-publications.com 10.3892 mmr.2020.11474 Aims and Scope Abstracting and Indexing Bibliographic Information Information for Librarians Information for Advertisers Reprints and permissions Contact the Editor General Information About Spandidos Conferences Job Opportunities Contact Terms and Conditions [Retracted] p53 suppresses stressu2011induced cellular senescence via regulation of autophagy under the deprivation of serum Authors: Xinbing Sui Yong Fang Haizhou Lou Kaifeng Wang Yu Zheng Fang Lou Wei Jin Yinghua Xu Wei Chen Hongming Pan Xian Wang Weidong Han View Affiliations Published online on: September 1, 2020 https: doi.org 10.3892 mmr.2020.11474 Copyright : u00a9 Sui et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0]. Metrics: Total Views: 3 (Spandidos Publications: 3 | PMC Statistics: 0 ) Total PDF Downloads: 3 (Spandidos Publications: 3 | PMC Statistics: 0 ) Cited By (CrossRef): 0 citations Article Following the publication of this article, an interested reader drew to the Editoru2019s attention that certain GAPDH control bands were strikingly similar, comparing between two different figures in the paper. Two GAPDH bands in Fig. 1D appeared to be duplicates of two GAPDH bands featured in Fig. 3B, although different conditions were represented by these figures. Furthermore, the protein bands featured in the two lanes in the left p62 panel in Fig. 1D, when flipped horizontally, looked remarkably similar to the Cyclin B lanes featured in the right-hand gel of Fig. 3B. Finally, the same protein bands were also strikingly similar to bands featured in Fig. 4B, albeit in a different experimental context. The Editor of Molecular Medicine Reports has investigated this matter, and we were able to confirm that the two sets of data bands featured in this trio of figures were indeed the same ones, beyond all reasonable doubt. Consequently, the Editor has decided that this article should be retracted from the publication on the basis of an overall lack of confidence in the presented data. The Editor apologizes to the readership of the Journal for any inconvenience caused. [the original article was published in Molecular Medicine Reports 11: 1214-1220, 2015; DOI: 10.3892 mmr.2014.2853] ,