MiRNA-802 inhibits the metastasis of colorectal cancer by targeting FOXE1
Authors: L-N Ma
Y Zhang
Y Xie
Journal: European review for medical and pharmacological sciences (2020)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: microRNA-802; colorectal cancer; FOXE1; progression; metastasis
Affiliations: Department of Traditional Chinese Medicine, Shanxi Cancer Hospital, Taiyuan, China. .

Abstract:  Objective: The aim of this study was to explore the role of microRNA-802 (miRNA-802) in the progression of colorectal cancer (CRC) and the underlying mechanism. Patients and methods: The relative expression levels of miRNA-802 and FOXE1 in 40 paired CRC tissues and adjacent normal tissues were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The correlation between miRNA-802 expression and the pathological indexes of CRC patients was assessed. Meanwhile, the prognostic potentials of miRNA-802 and FOXE1 in CRC patients were identified through the Kaplan-Meier method. After overexpression of miRNA-802, the changes in the proliferative, migratory, and invasive capacities of HT29 and HCT-8 cells were evaluated in vitro. The Dual-Luciferase Reporter Gene Assay was applied to investigate the binding relationship between miRNA-802 and FOXE1. Finally, the rescue experiments were carried out to uncover the role of the miRNA-802/FOXE1 axis in regulating the cellular behaviors of CRC. Results: MiRNA-802 was significantly downregulated in CRC tissues and cell lines. CRC patients with a low level of miRNA-802 had significantly higher rates of lymphatic metastasis and distant metastasis, as well as worse overall survival. The transfection of miRNA-802 mimics remarkably attenuated the proliferation, migration, and invasion of HT29 and HCT-8 cells. FOXE1 expression was significantly upregulated in CRC tissues and cell lines. Meanwhile, the expression of FOXE1 was negatively correlated with miRNA-802 in CRC tissues. A higher level of FOXE1 indicated the worse prognosis of CRC patients. The Dual-Luciferase Reporter Gene Assay further verified the binding relationship between FOXE1 and miRNA-802. Importantly, the overexpression of FOXE1 could reverse the regulatory effects of miRNA-802 on the cellular behaviors of CRC. Conclusions: MiRNA-802 is significantly downregulated in CRC, and is closely related to lymphatic and distant metastasis of CRC. Furthermore, miRNA-802 alleviates the malignant progression of CRC via negatively regulating FOXE1. 

Retracted: No

PubPeer comments:
 Fig 3C:  A negative correlation between expression levels of miRNA-802 and FOXE1 in CRC tissues . Rescaled for comparison with (right) a superimposition of 20 versions of the scatterplot, from different papers from a papermill. Darker data-points are present in more of the variants. ![file] , 
 Another frequent characteristic feature of the papermill s productions are pairs of K-M plots, resembling combs or walls of Lego, with a precipitous drop in survival at 75 months, Here, Figs 1C and 3D.  Kaplan-Meier curves revealed the overall survival in CRC patients with high and low expression of miRNA-802 ...  Kaplan-Meier curves revealed the overall survival in CRC patients with high and low expression of FOXE1 . ![file] ,