LncRNA PCAT6 promotes occurrence and development of ovarian cancer by inhibiting PTEN
Authors: H-Y Zhang
S-E Liu
Y Zhou
Y-H Lv
F-R Kong
H-M Yao
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: lncRNA PCAT6; ovarian cancer; expression; function; metastasis
Affiliations: Department of Gynecology, Affiliated Hospital of Jining Medical University, Jining, China. .

Abstract:  Objective: The purpose of this study was to explore the expression and function of long non-coding RNA (lncRNA) PCAT6 in ovarian cancer. Patients and methods: Quantitative Real-Time-Polymerase Chain Reaction (qRT-PCR) was used to detect the expression of lncRNA PCAT6 in 42 pairs of ovarian cancer tissues and adjacent normal tissues. Then, the relationship between PCAT6 expression and pathological indicators of ovarian cancer was analyzed. Subsequently, the transfection efficiency of PCAT6 in ovarian cancer cells was verified, and the PCAT6 knockdown model was constructed using lentiviruses in SKOV3 and CAOV3 ovarian cancer cell lines. In addition, Cell Counting Kit-8 (CCK-8) test, wound healing assay and transwell invasion and migration experiments were performed to estimate the effect of PCAT6 on the biological function of ovarian cancer cells, to further explore the possible potential mechanisms. Results: QRT-PCR results showed that the expression level of PCAT6 in ovarian cancer was higher than that in the adjacent normal tissues. The incidence of distant metastasis and lymph node metastasis in patients with high expression of PCAT6 was higher than those with low PCAT6 expression. Compared with the NC group, the proliferation, metastasis and invasion ability of ovarian cancer cells in si-PCAT6 group decreased significantly. QRT-PCR results demonstrated that the PTEN expression was increased after the knockdown of PCAT6. In addition, the recovery experiment also revealed that PCAT6 and PTEN have a mutual regulation, which can jointly regulate the development of ovarian cancer. Conclusions: LncRNA PCAT6 was up-regulated in ovarian cancer tissues and was closely related to distant metastasis or lymph node metastasis. Additionally, lncRNA PCAT6 might promote the proliferation, migration and invasion of ovarian tumor cells by inhibiting PTEN. 

Retracted: No

PubPeer comments:
 Table 1 has column headings promising to convert the numbers within patient subgroups into percentages, but without delivering. ![file] Of course this is not a grievous error. However, Table 1s of this form are a recurring feature of the outputs of a papermill. Other products are tabulated in a Google spreadsheet: https: docs.google.com spreadsheets d 1_762PbYYTCJhek5PdBuiDuN1q48UDgMa-rT6gi_jA8c edit?usp=sharing Another example here, from [Yang, Wang Luo (2018)] . At random. ![] , 
 Fig 2C.  Cell scratch assay to test the effect of SKOV3 and CAOV3 cell lines on ovarian cancer cell healing . Lots of repeated panels are visible when contrast is enhanced. ![file] , 
 *[above]* Fig 4D. Taken from the 4-hour and 12-hour columns of *[below]* Fig 8A B from  Potent Inhibition of miR-34b on Migration and Invasion in Metastatic Prostate Cancer Cells by Regulating the TGF-u03b2 Pathway ([Fang et al 2017] ). ![] ,