ADAM10 is overexpressed in human hepatocellular carcinoma and contributes to the proliferation, invasion and migration of HepG2 cells
Authors: Shengli Wu
Shao Yuan
Shi Lei
Journal: Oncology Reports (2013)
Discipline: CLINICAL MEDICINE
Keywords: ADAM10; hepatocellular carcinoma; proliferation; invasion; migration
Affiliations: Department of Otorhinolaryngology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.

Abstract:  The overexpression of A disintegrin and metalloproteinase 10 (ADAM10) has been found to be closely associated with the development and progression of various types of tumors. However, ADAM10 expression in hepatocellular carcinoma (HCC) and its significance remain largely unknown. The present study aimed to investigate the expression of ADAM10 in human HCC and the effect of ADAM10 gene silencing by siRNA on the proliferation, invasion and migration of HepG2 human hepatoma cells. Immunohistochemistry was performed to examine the expression of ADAM10 in human HCC tissues and in the adjacent non-cancer tissues from 30 patients with HCC. RNA interference was used to knock down ADAM10 expression in HepG2 human hepatoma cells and the proliferation and migration as well as the invasive ability of the treated cells were observed in vitro. The expression of ADAM10 protein in HCC tissues was significantly higher when compared to that in adjacent non-tumor tissues (P&lt;0.05). The high expression of ADAM10 in cancer was significantly correlated with clinical outcomes (P&lt;0.05). Silencing of ADAM10 resulted in inhibition of proliferation and migration as well as invasion of HepG2 human hepatoma cells (P&lt;0.05). These studies suggest that ADAM10 plays an important role in regulating proliferation, invasion and migration of HepG2 cells. High expression of ADAM10 may be a valuable predictive factor for HCC prognosis, and ADAM10 is potentially an important therapeutic target for the prevention of tumor development and progression in HCC. 

Retracted: No

PubPeer comments:
 Fig 6A.  A Matrigel Transwell invasion assay was used to test the migration ability of the cells (a, blank control group; b, Lipo2000 group; c, control siRNA group and d, ADAM10-siRNA group) to pass through the basement membrane. (b) and (c) do not seem to represent different treatments. ![file] , 
 Dear Hoya Camphorifolia Thank you very much for pointing out the negligence in our published work. With the help of the pathologist in our hospital by reviewing the original experimental pictures, we admitted that it is indeed a misused picture for Fig 6A(c). We have written to Spandidos publications to request erratum. Thank you again for discovering our mistake in time and helping us to correct it. Best regards, Shengli Wu , 
 https: pubpeer.com publications E32376FB1D595110D32D245CA1B60B ![file] , 
 So the images appear in -  [Downregulation of ADAM10 expression inhibits metastasis and invasiveness of human hepatocellular carcinoma HepG2 cells]  (Yue et al 2013): the version retracted from BioMed Research International. - the present paper: earliest, and cited as the reason for the retraction. -  [Synergistic effects of co-expression plasmidu2011based ADAM10-specific siRNA and GRIM-19 on hepatocellular carcinoma in vitro and in vivo]  (Liu et al 2014). Have I missed anything? , 
 Dear Hoya Camphorifolia 1.tu201cDownregulation of ADAM10 expression inhibits metastasis and invasiveness of human hepatocellular carcinoma HepG2 cells (Yue et al 2013)u201dwas retracted from BioMed Research International as it is found to contain a substantial amount of material, without referencing, from our paper. 2.t Synergistic effects of co-expression plasmid based ADAM10-specific siRNA and GRIM-19 on hepatocellular carcinoma in vitro and in vivo (Liu et al 2014) shares some images with our paper. We didn t know about it until we learned about it from PubPeer. We reserve the right to defend our intellectual property rights. Best regards, Shengli Wu , 
 #5 I agree, it is clear that you have been the victims of plagiarism. ,