Analgesic effects of microRNA‐129‐5p against bone cancer pain through the EphB1/EphrinB2 signaling pathway in mice
Authors: Guo-Qing Zhao
Shao-Nan Yu
Lin Liu
Xue-Feng Li
Gui-Feng Liu
Long-Yun Li
Journal: Journal of Cellular Biochemistry (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: EphB1/EphrinB2 signaling pathway, microRNA-129-5p; analgesic effects; bone cancer pain.
Affiliations: Department of Radiology China‐Japan Union Hospital of Jilin University Changchun People’s Republic of China, Department of Anesthesiology China‐Japan Union Hospital of Jilin University Changchun People’s Republic of China

Abstract:  The study aims to investigate the analgesic effects of microRNA-129-5p (miR-129-5p) on bone cancer pain (BCP) by targeting Eph receptor B1 (EphB1) through the EphB1/EphrinB2 signaling pathway. BCP mice models were established, and C3H/HeJ female mice were classified into the normal, blank, negative control (NC), miR-129-5p mimics, miR-129-5p inhibitors, EphB1 knockout (KO), and miR-129-5p inhibitors + EphB1 KO groups. Quantitative reverse transcription polymerase chain reaction and Western blot analysis were used to evaluate the miR-129-5p expression, and messenger RNA (mRNA) and protein expressions of EphB1, p-EphB1, EphrinB2, and p-EphrinB2. EphB1 and EphrinB2 were highly activated in the tibias of BCP mice 7 days after the operation. EphB1 is a target gene of miR-129-5p. The mechanical withdrawal threshold increased in the miR-129-5p mimics, EphB1 KO and miR-129-5p inhibitors + EphB1 KO groups, but decreased in the miR-129-5p inhibitors group. Compared with the blank and the NC groups, the expression of miR-129-5p was significantly increased in the miR-129-5p mimics group, and the mRNA and protein expressions of EphrinB2, p-EphrinB2, EphB1, and p-EphB1 were significantly decreased, while in the miR-129-5p inhibitors group, the results were opposite (all P &lt; 0.05); the mRNA and protein expressions of EphrinB2, p-EphrinB2, EphB1, and p-EphB1 were significantly decreased in the EphB1 KO group (all P &lt; 0.05); the expression of miR-129-5p was significantly decreased in the miR-129-5p inhibitors + EphB1 KO group ( P &lt; 0.05), while the mRNA and protein expressions of EphrinB2 and p-EphrinB2 were not significantly different ( P &gt; 0.05). The results indicated that upregulated miR-129-5p alleviate BCP via downregulation of the EphB1/EphrinB2 signaling pathway. 

Retracted: Yes

PubPeer comments:
 Fig 6B.  western blotting of protein and expressions of EphrinB2, p-EphrinB2, EphB1 and p-EphB1 . ![file] The protein blobs seem to come from the same restricted gamut shown in this montage: ![] The stylised nature of these Western Blots are suggestive of a papermill provenance for this report. Other examples are tabulated in a spreadsheet. https: docs.google.com spreadsheets d 1r0MyIYpagBc58BRF9c3luWNlCX8VUvUuPyYYXzxWvgY edit?usp=sharing , 
 Retracted [19 July 2021] . ---------------------------------------------- The above article, published online on 13 December 2017 in Wiley Online Library has been retracted by agreement between the authors, the journal s Editor in Chief, Prof. Dr. Christian Behl, and Wiley Periodicals LLC. The retraction has been agreed following an investigation based on allegations raised by a third party. A detailed investigation revealed that several image elements of the experimental data were published elsewhere in a different scientific context. The conclusions of this article are invalid. ,