The inhibition of tumor protein p53 by microRNA-151a-3p induced cell proliferation, migration and invasion in nasopharyngeal carcinoma
Authors: He Xu
Yaping Xu
Haibin Liu
Juntian Lang
Yin Cheng
Jingping Fan
Zheng Lin
Journal: Bioscience Reports (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Apoptosis; Flow cytometry; Luciferase reporter assay; Matrix metalloproteinases.
Affiliations: Department of ENT&N Surgery, Shanghai Changzheng Hospital, No. 415 Fengyang Road, Huangpu District 200003, Shanghai, China., Department of ENT&N Surgery, No.452 Hospital, Jinjiang District 610000, Chengdu, China.

Abstract:  A close relation between microRNA-151a-3p (miR-151a-3p) and nasopharyngeal carcinoma (NPC) has been reported, however, the molecular mechanism is still unclear. The aim of the present study was to explore the mechanism in the promotion of miR-151a-3p to NPC progression. The levels of miR-151-3p in several NPC cell lines were detected in order to screen an experimental cell line. MiR-151a-3p mimic and inhibitor were constructed and transfected into 5-8F cells and cell proliferation were detected by Cell Counting Kit-8 (CCK-8). The apoptosis rate, cell migration and invasion were determined by flow cytometry, wound healing and Transwell assays. The predicted target was further verified by luciferase reporter assay. Real-time quantification-PCR and Western blot were carried out for mRNA and protein level analysis. Tumor protein p53 was co-transfected to verify the functions of miR-151a-3p. The miR-151a-3p level in NPC tissues was much higher than that in adjacent tissues. After transfecting cells with miR-151a-3p mimic, the cell proliferation and patients' survival rate were much increased, and this was accompanied by the increase in B-cell lymphoma 2 (Bcl-2) and decreases in Bax and cleaved caspase-3 (P&lt;0.01). Moreover, the migration rate and number of invaded cells were also remarkably increased, however, the miR-151a-3p inhibitor had opposite effects on the 5-8F cells. Noticeably, p53 was revealed as a potential target of miR-151a-3p. Co-transfection of P53 could partially reverse the promotive effects of miR-151a-3p on NPC cell progression. Our data indicated that blocking p53 expression and mediated signal pathways contribute to the positive effects of miR-151a-3p on NPC cell proliferation, migration and invasion. 

Retracted: Yes

PubPeer comments:
 Figs 2C, 3C, 7D. ![file] One panel (purple rectangle) is surprisingly similar to panels from Figs 2D, 5D, 6D of [Zhao et al (2018)] and Figs 2C, 4C, 5D of [Lyu et al (2019)] . The panels are not identical but they share a majority of points and seem to have come from the same raw data. ![file] Here in context: ![] ![] , 
 Retracted, [29 October 2021] . ----------------------------- This article is being retracted from *Bioscience Reports* at the request of the Editor-in-Chief and the Editorial Board following receipt of a notification from a reader, alerting the Editorial Board to the inhibitor panel in Figure 3C, which contains a significant number of data points overlapping with that from figures in other publications by different authors. The authors have been contacted in regards to the Retraction, and have not responded to the Journal s queries and the concerns raised. Given the extent of the issues raised, the Editorial Board stand by the decision to retract the article. ,