An array of 60,000 antibodies for proteome-scale antibody generation and target discovery
Authors: Shiwei Wang
Ziqing Chen
Jing Geng
Luyan Zulie Ma
Fei Lin
Weining Weng
Qingyou Xia
Nan Wang
Yiqiang Wang
Jian Yan
Qin Zhang
Yangrui Li
Bing Hou
Sheng-Ce Tao
Mira I Pronobis
Huaping Dai
Yang Li
Kun Wang
Wenfang Zeng
Xiquan Zhang
Zhiqing Li
Guangcun Cheng
Susan Zheng
Ying Zhang
Rong Pan
Xuefan Xu
Zhaohui Wang
Yuemeng Wang
Dongliang Huang
Li Jiang
Yuxian Zhu
Yuan Yu
Qinghua Nie
Fulin Chen
Zhiqiang Wang
Yanfang Tang
Kenneth D Poss
Xiao-Dong Zhao
Xuemei Du
Zheng Yuan
Ying Lin
Mingqiao Wang
Xun Meng
Journal: Science advances (2020)
Discipline: Multidisciplinary
Keywords: Antibodies; Proteome; Epitope; Antibody Library; Monoclonal antibodies
Affiliations: School of Life Sciences, Northwest University, Xi'an, Shanxi 710069, China., Abmart, 333 Guiping Road, Shanghai 200033, China., Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China., Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA., State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China., Guangxi Key Laboratory of Sugarcane Genetic Improvement/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Nanning, Guangxi 530007, China., Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China., Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan 450009, China., Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100083, China., State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China., Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, China-Japan Friendship Hospital, National Clinical Research Center for Respiratory Diseases, Beijing 100029, China., School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China., School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China., Ludwig Institute for Cancer Research, 9500 Gilman Dr., La Jolla, CA 92093, USA., Department of Medical Biochemistry and Biophysics, Division of Functional Genomics and Systems Biology, Karolinska Institutet, 171 65 Stockholm, Sweden., College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, China., Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, China., Institute for Advanced Studies and College of Life Sciences, Wuhan University, Wuhan, China., State Key Laboratory of Oncogenes and Related Genes, Shanghai 200240, China.

Abstract:  Antibodies are essential for elucidating gene function. However, affordable technology for proteome-scale antibody generation does not exist. To address this, we developed Proteome Epitope Tag Antibody Library (PETAL) and its array. PETAL consists of 62,208 monoclonal antibodies (mAbs) against 15,199 peptides from diverse proteomes. PETAL harbors binders for a great multitude of proteins in nature due to antibody multispecificity, an intrinsic antibody feature. Distinctive combinations of 10,000 to 20,000 mAbs were found to target specific proteomes by array screening. Phenotype-specific mAb-protein pairs were found for maize and zebrafish samples. Immunofluorescence and flow cytometry mAbs for membrane proteins and chromatin immunoprecipitation-sequencing mAbs for transcription factors were identified from respective proteome-binding PETAL mAbs. Differential screening of cell surface proteomes of tumor and normal tissues identified internalizing tumor antigens for antibody-drug conjugates. By finding high-affinity mAbs at a fraction of current time and cost, PETAL enables proteome-scale antibody generation and target discovery. 

Retracted: No

PubPeer comments:
 I am receiving unsolicited and inappropriate spam from the employers of this paper s corresponding author, citing the paper as the reason why I should buy their products. ![file] *Dear Sir or Madam:* *Recently, our scientists in Abmart reported a new antibody microarray screening technology named PETALu2122 (Proteome Epitope Tag Antibody Library) in Science Advances (An Array of 60,000 Antibodies for Proteome-Scale Antibody Generation and Target Discoveryuff0chttps: advances.sciencemag.org content 6 11 eaax2271 ). PETAL combines the advantage of antibody multispecificity (refer to Cell 1997, 91 6:799-809) and high throughput screening ability of protein microarray, thus enables fast and proteome-scale antibody discovery.* *If you are interesting in get more information you are welcome to leave us a message at weining.weng@ab-mart.com, or visit us at http: www.ab-mart.com http: t.email.ab-mart.com t.aspx subid 756840709 camid 1710452 linkid 6468957 Default.aspx .* The spam is signed by  weining.weng@ab-mart.com , whom I assume to be the co-author Weining Weng. Can the authors assure us that the paper was not published to facilitate this random bombardment with advertising spam? ,