Retracted : microRNA‐129‐5p involved in the neuroprotective effect of dexmedetomidine on hypoxic‐ischemic brain injury by targeting COL3A1 through the Wnt/β‐catenin signaling pathway in neonatal rats
Authors: Xiu-Min Zhou
Jie Liu
Jian Pei
Shu-Li Zhang
Xiang Xu
Man-He Zhang
Xin Zhao
Ying Wang
Journal: Journal of Cellular Biochemistry (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Wnt/β-catenin signaling pathway; dexmedetomidine; hypoxic-ischemic brain injury; hypoxic-ischemic neonatal rats; microRNA-129-5p.
Affiliations: Department of Anesthesiology Tangshan Gongren Hospital Tangshan China, Department of Neurosurgery Tangshan Gongren Hospital Tangshan China

Abstract:  Our study aims to elucidate the mechanisms how microRNA-129-5p (miR-129-5p) involved in the neuroprotective effect of dexmedetomidine (DEX) on hypoxic-ischemic brain injury (HIBI) by targeting the type III procollagen gene (COL3A1) through the Wnt/β-catenin signaling pathway in neonatal rats. A total of 120 rats were obtained, among which 15 rats were selected as sham group and rest rats as model, DEX, DEX + negative control (DEX + NC), DEX + miR-129-5p mimics, DEX + miR-129-5p inhibitors, DEX + XAV-939, and DEX + miR-129-5p inhibitors + XAV-939 groups. A dual-luciferase reporter assay was performed for the target relationship between miR-129-5p and COL3A1. Weight rate and water content of cerebral hemisphere were detected. Quantitative real-time polymerase chain reaction and Western blot analysis were conducted to detect miR-129-5p expression and expressions of COL3A1, E-cadherin, T-cell factor (TCF)- 4, and β-catenin. The DEX, DEX + miR-129-5p mimics, DEX + XAV-939 groups had increased weight rate of the cerebral hemisphere, but decreased water content of left cerebral hemisphere, levels of COL3A1, β-catenin, TCF-4, and E-cadherin in the hippocampus compared with the model and DEX + miR-129-5p inhibitors groups. COL3A1 was verified as the target gene of the miR-129-5p. Compared with the DEX + NC and DEX + miR-129-5p inhibitors + XAV-939 groups, the DEX + XAV-939 and DEX + miR-129-5p mimics groups had elevated weight rate of the cerebral hemisphere, but reduced water content of left cerebral hemisphere, levels of COL3A1, β-catenin, TCF-4, and E-cadherin in the hippocampus. Our findings demonstrate that miR-129-5p improves the neuroprotective role of DEX in HIBI by targeting COL3A1 through the Wnt/β-catenin signaling pathway in neonatal rats. 

Retracted: Yes

PubPeer comments:
 Two images in Figure 8A appear to show the same field of view. Shown with increased brightness. *** ![file] , 
 #1 Dear Dr Actinopolyspora Biskrensis, Thank you very much for pointing out the mistake of the manuscript. According to your concern, we carefully checked the raw data of the research. In Figure 8A the control and the SD+SB image show the same field of view. This may be due to that we made an error in the process of naming the picture during the experiment. We sincerely apologize for that this error was not found during the proof of the manuscript. We will contact the editor of the journal and correct the mistake as soon as possible. We apologize again for the careless mistake and we really appreciate your great helps. Yours sincerely, Xiufeng Yu. , 
 Erratum: https: www.cellphysiolbiochem.com Articles 000484 --------------------------- In the article u201cModulation of Pulmonary Vascular Remodeling in Hypoxia: Role of 15-LOX-2 15- HETE-MAPKs Pathwayu201d [Cell Physiol Biochem 2015;35:2079-2097. DOI: 10.1159 000374015] by Yu et al., the incorrect representative images were included in Figure 4A and 8A as a result of unintentional errors in the naming process of data storage which caused data to appear in different groups. In Figure 4A, the same representative trace was presented for HYP+U and HYP+SB. The representative trace for HYP was presented as the trance for HYP+SP. The correct representative traces for the HYP+U, HYP+SB and HYP+SP groups is shown in the corrected Figure 4 below. The corrected Figure 4 and Figure 8 are shown here. ![] ![] ,