Efficient tumor targeting by polysaccharide decked polyethylenimine based nanocomposites
Authors: Atul Pathak
Anil K Mishra
Krishna Chuttani
Kailash C Gupta
Archana Swami
Sanyog Jain
Pradeep Kumar
Suresh P Vyas
Soma Patnaik
Journal: Journal of Biomedical Nanotechnology (2009)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: Hyaluronic acid; polyethylenimine; nanocomposites; solid tumors; transfection efficiency
Affiliations: Institute of Genomics and Integrative Biology (CSIR), Delhi University Campus, Mall Road, Delhi 110007, India.

Abstract:  Hyaluronic acid (HA)-polyethylenimine (PEI, 25 kDa) (HP) nanocomposites were fabricated for efficient targeting to solid tumors. Branched PEI was ionically blended with a natural mucopolysaccharide, HA, to partially block the positive charge and to impart site specificity to HP nanocomposites. A series of nanocomposites were prepared by varying the content of HA. HP nanocomposites were characterized by their size, morphology, zeta potential and evaluated for pDNA protection study, transfection efficiency and cytotoxicity. The competency of HP nanocomposites to relocate a plasmid encoding enhanced green fluorescent protein (pEGFP) gene was assessed in HEK293, HEK293T, and HeLa cells and found to be approximately 1-8 folds efficient compared to Superfect, Fugene, GenePORTER 2. HP nanocomposites also exhibited efficient transfection in serum-containing medium. MTT assay showed significantly improved cell viability in HEK293T, HepG2 and HeLa cells. The specificity of HP nanocomposites to target tumor was investigated in vivo by injecting pDNA-loaded HP-4 nanocomposite or PEI intravenously into mice bearing Ehrlich ascites tumor (EAT). The gamma scintigraphic studies showed a higher accumulation of HP-4 nanocomposite in the solid tumor compared to PEI. The results cumulatively advocate that HP nanocomposites could epitomize a viable alternative for site specific gene therapy. 

Retracted: No

PubPeer comments:
 The author proposed in this paper **Pythagorean fuzzy Hamacher power aggregation operators** and claimed that they are monotonic, which can be easily proved. However, **this is simply not the case**, because the original version of power aggregation operators proposed by Yager is known to be not monotonic. See also https: pubpeer.com publications E2951D1B90C4B63B92D6675ACC64B1 https: pubpeer.com publications 3B9236D4BDD6177F4D5085195AC445 https: pubpeer.com publications BA2AE54716F248CA641312AD85EB48 ![file] ![file] ![file] , 
 This paper has a sole author G.W. Wei and was submitted on August 2017. It shares the same patterns of closing sentences and citing styles. The reference list includes 82 papers of which 42 are authored by G.W. Wei and his co-authors. ![file] , 
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