MiR-30 suppresses lung cancer cell 95D epithelial mesenchymal transition and invasion through targeted regulating Snail
Authors: M-J Fan
S-K Wang
G-H Zhao
Y Zhang
W Shen
Y-H Zhong
K-F Yuan
Journal: European review for medical and pharmacological sciences (2017)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: Snail; miR-30a; NSCLC; EMT; invasion
Affiliations: Department of Respiratory Medicine, The Second Affiliated Hospital, Kunming Medical University, Kunming, Yunnan, China. .

Abstract:  Objective: As an important factor regulating the epithelial mesenchymal transition (EMT) Snail is associated with lung cancer. Bioinformatics analysis showed that microRNA-30a (miR-30a) may target the 3'-UTR of Snail mRNA. It was exhibited that miR-30a down-regulation was related to tumor size, TNM stage, and poor prognosis of non-small cell lung cancer (NSCLC) patients, which suggests that miR-30a might participate in NSCLC attack. This study aims to explore the role of miR-30a and Snail in NSCLC invasion and metastasis. Patients and methods: NSCLC tumor and para-carcinoma tissues were collected from 46 patients to evaluate the miR-30a and Snail expressions. The targeted relationship between miR-30a and Snail was verified by using dual-luciferase reporter assay. 95D cells were cultured in vitro and transfected with miR-30a mimic or small interfere RNA targeting Snail (si-Snail). The expression of miR-30a, Snail, EMT-related factors, malignant growth, invasion, and apoptosis, were compared. Results: Snail was significantly up-regulated, while miR-30a was significantly reduced in NSCLC tissue. MiR-30a suppressed Snail expression by targeting the 3'-URT of Snail mRNA. 95D cells exhibited significantly higher Snail, N-cadherin, and vimentin levels, while lower miR-30a, E-cadherin, and occludin expressions were compared with 95C cells. 95D cells presented stronger malignant growth and invasive ability, whereas lower background apoptosis than 95C. MiR-30a mimic and/or si-Snail transfection significantly enhanced E-cadherin and occludin expression, while significantly declined N-cadherin and vimentin levels, thus weakening malignant growth and invasion and increasing cell apoptosis. Conclusions: Snail up-regulated, while miR-30a declined in NSCLC tissue. MiR-30a may suppress Snail expression, restrain EMT, and inhibit lung cancer cell invasion. 

Retracted: Yes

PubPeer comments:
 Western Blots are sharp-edged matt-black ellipses on a smooth gray background. For instance, Fig 4B. ![file] , 
 Withdrawn. https: www.europeanreview.org article 22986 *Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cMiR-30 suppresses lung cancer cell 95D epithelial mesenchymal transition and invasion through targeted regulating Snail, by M.-J. Fan, Y.-H. Zhong, W. Shen, K.-F. Yuan, G.-H. Zhao, Y. Zhang, S.-K. Wang, published in Eur Rev Med Pharmacol Sci 2017; 21 (11): 2642-2649u2013PMID: 28678320u201d has been withdrawn.* *The Publisher apologizes for any inconvenience this may cause.* , 
 [This article] appears to refer to this paper. Cut and pasted (translated via Google Translate, which may include errors)  In order to strengthen the integrity of scientific research, severely punish violations, and create a good environment for scientific and technological innovation, we hereby report the relevant paper issues recently investigated and dealt with by this unit as follows. The paper  MiR-30 suppresses lung cancer cell 95D epithelial mesenchymal transition and invasion through targeted regulating Snail was published by Zhong Yunhua, the corresponding author of the First People s Hospital of Yunnan Province and Fan Minjuan of the Second Affiliated Hospital of Kunming Medical University as the first author. It is suspected of falsification of the picture. There is no original record of the production process of the suspected picture (Western Western Blot). The picture was provided by a third-party organization. This organization only completed part of the experiment and provided the experimental results. There is no cooperation agreement between the two parties and the original record of the third-party experiment. The First Peopleu2019s Hospital of Yunnan Province dealt with the relevant person in charge Zhong Yunhua: (1) Cancel the application for reviewing professional and technical titles for 2 years; (2) Suspend job promotion for 2 years; (3) Cancel the application for science and technology plans (special projects, funds, etc.) projects , Enrollment for master s degree candidates and other qualifications for 3 years. At the same time, it is recorded in the scientific research integrity information system, and the hospital urges it to rectify and strengthen the management of the academic team. Administrative handling: exhortation of talks, notification of criticism in the hospital. The Second Affiliated Hospital of Kunming Medical University dealt with the relevant responsible personnel: (1) Admonishment talks to Fan Minjuan (1st work), and the hospital notified criticism. (Completed) (2) Recover the amount of paper awards and article page fee reimbursements (completed); (3) Order the parties to withdraw the papers in question. (Completed) (4) It is recommended that the higher-level competent authority cancel Fan Minjuan s qualification as the chief physician. It is not allowed to apply for the qualification for evaluating professional and technical titles within two years from the date of cancellation. (5) Cancel Fan Minjuan s (first work) application qualifications for applying for various scientific research projects at all levels and signing of cooperative projects within five years; cancel her qualifications for enrolling graduate students within five years. (6) Talk to Shen Wen (three works), Yuan Kaifen (four works), Zhao Guohou (five works), Zhang Yang (six works), and Wang Shukun (seven works). (7) Cancel Shen Wen (three works), Yuan Kaifen (four works), Zhao Guohou (five works), Zhang Yang (six works), Wang Shukun (seven works) to apply for the qualifications of various scientific research projects at all levels and the signing of cooperation projects within two years qualifications. ,