Long non-coding RNA 91H regulates IGF2 expression by interacting with IGF2BP2 and promotes tumorigenesis in colorectal cancer
Authors: Bangshun He
Yuqin Pan
Xiangxiang Liu
Tianyi Gao
Shukui Wang
Journal: Artificial Cells Nanomedicine and Biotechnology (2020)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: CRC; IGF2; IGF2BP2; lncRNA 91H.
Affiliations: Department of Clinical Laboratory, Nanjing First Hospital, Nanjing Medical University, Nanjing, China., Central Laboratory, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.

Abstract:  91H, a long non-coding antisense transcripts located on the position of the H19/IGF2 locus had been suggested to play a critical role in tumour development. However, little study had proved the mechanism in colorectal cancer (CRC). Hence, we performed this study to deeply explore the mechanism of lncRNA 91H in tumour progression. The expression of lncRNA 91H was first detected in CRC tissues and cells which was higher in vitro and in vivo than normal cells or tissues and CRC patients with high lncRNA 91H expression usually had a high risk in tumour metastasis (p &lt; .05). Then, monodansylcadaverine (MDC) staining, scratch wound, migration and invasion assays were conducted which showed to that reduced lncRNA 91H would greatly affect tumour migration, invasion and autophagy. Finally, by RNA pull down and RNA-binding protein immunoprecipitation (RIP) assay, a significant interaction was found between lncRNA 91H and IGF2BP2 which was proved to play an important role in CRC IGF2 expression. All these results suggested lncRNA 91H promotes IGF2 expression by interacting with IGF2BP2 which would provide a new strategy in finding potential CRC diagnostic biomarkers and therapeutic targets. 

Retracted: Yes

PubPeer comments:
 Figure 1A appears to have images which overlap. TAM. *** ![file] , 
 Corresponding author appears to be in the leadership group of their institution. Would be nice to see a response addressing this concern (and with other papers). *** ![file] , 
 Dear Dr. Actinopolyspora Biskrensis, Thank you very much for your important comments on our studies. We take your inqueries seriously. We are investigating and verifying the original records and raw data. We will assess the effect of these issues on the conclusion of the publications you listed, and will contact the editorial offices to correct the mistakes as necessary. We really appreciate your comments on our works and will response soon after. Best regards , 
 **Retracted** February 3, 2022 u201cWe, the Editors and Publisher of the journal Artificial Cells, Nanomedicine, and Biotechnology, have retracted the following article: Tianyi Gao, Xiangxiang Liu, Bangshun He, Yuqin Pan Shukui Wang (2020) Long non-coding RNA 91H regulates IGF2 expression by interacting with IGF2BP2 and promotes tumorigenesis in colorectal cancer, Artificial Cells, Nanomedicine, and Biotechnology, 48:1, 664-671, DOI: 10.1080 21691401.2020.1727491 Since publication, the authors (sic) noticed duplication in Figure 1A and 4A. As this error directly impacts the reported results and conclusions the authors alerted the issue to the Editor and Publisher to request correction. The authors acknowledged that they had made mistakes and the Editor and Publisher found there to be fundamental flaws present within the requested corrections, as well as the rest of the paper. The authors have agreed to the retraction of this article. We have been informed in our decision-making by our policy on publishing ethics and integrity and the COPE guidelines on retractions. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as u2018Retractedu2019. u201c ,