TRPM channels mediate zinc homeostasis and cellular growth during Drosophila larval development
Authors: David Wright
Cecile Martel
Plamen Georgiev
Hanneke Okkenhaug
Valentina Carta
Padinjat Raghu
Johannes Oberwinkler
Anna Drews
Sachar Lambert
Melanie Flick
Journal: Cell Metabolism (2010)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: TRPM channels; physiological function; ionic permeability; Drosophila; divalent cations
Affiliations: Inositide Laboratory, Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK., Emmy Noether Research Group, Experimentelle und Klinische Pharmakologie und Toxikologie, Uniklinikum des Saarlandes, 66421 Homburg, Germany., Department of Physiology, Development, and Neuroscience, University of Cambridge, Cambridge CB2 3AT, UK., Inositide Laboratory, Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK. Electronic address: .

Abstract:  TRPM channels have emerged as key mediators of diverse physiological functions. However, the ionic permeability relevant to physiological function in vivo remains unclear for most members. We report that the single Drosophila TRPM gene (dTRPM) generates a conductance permeable to divalent cations, especially Zn(2+) and in vivo a loss-of-function mutation in dTRPM disrupts intracellular Zn(2+) homeostasis. TRPM deficiency leads to profound reduction in larval growth resulting from a decrease in cell size and associated defects in mitochondrial structure and function. These phenotypes are cell-autonomous and can be recapitulated in wild-type animals by Zn(2+) depletion. Both the cell size and mitochondrial defect can be rescued by extracellular Zn(2+) supplementation. Thus our results implicate TRPM channels in the regulation of cellular Zn(2+) in vivo. We propose that regulation of Zn(2+) homeostasis through dTRPM channels is required to support molecular processes that mediate class I PI3K-regulated cell growth. 

Retracted: No

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 More details at http: www.birdforum.net showthread.php?t=303435 , 
 See https: www.academia.edu 33827046 Final_investigation_on_serious_allegations_of_fabricated_and_or_falsified_data_in_Al-Sheikhly_et_al._2013_2015_-_1_July_2016 , 
 See https: www.researchgate.net publication 263688691_Breeding_ecology_of_the_Basra_Reed_Warbler_Acrocephalus_griseldis_in_Iraq_Aves_Passeriformes_Acrocephalidae reviews 113402 for an open review of this paper. , 
 Copy pasted from https: doi.org 10.13130 2282-5398 9073 (published on 2 November 2017):  A report with the findings of an investigation on serious allegations of fabricated and or falsified data in the study on the breeding biology of the Basra Reed Warbler was released on 1 July 2016 (Anonymous, 2016). The report was sent to COPE, to TF, to the EiC of Zoology in the Middle East, and to other parties, on the day when it was released. The report reveals that no university in Saudi Arabia had endorsed the study. The report reveals that all efforts failed to get access to the raw research data. The report confirms the serious allegations that the study on the breeding biology of the Basra Reed Warbler contains fabricated and or falsified data. Repeated requests to COPE, to TF and to other parties, for example to members of the board of OASPA (the Open Access Scholarly Publishers Association4),for views of experts (within this field of research) who refute the main findings of this report remained unanswered. Similar requests to biologists of the University of Western Australia remained unanswered. Similar requests to other parties remained unanswered as well. It seems thus reasonable to argue that there are no experts who refute that the fatally flawed study (Al-Sheikhly *et al*, 2013, 2015) contains fabricated and or falsified data. The study thus must be retracted, and as soon as possible. Montgomery and Oliver (2017) argue that the scientific body of knowledge will get contaminated when fraudulent studies are not removed from the scientific body of knowledge (i.e. when they are not retracted). Fazaa* et al*. (2017) is an example that this contamination is already happening. , 
 https: osf.io 5pnk7 (created 1 April 2018): , 
 See also https: osf.io cvu5b , 
 The issues with this paper were also noted by the Iraqi Ministry of Environment. As a result, this Ministry has initiated an extensive inquiry about the credibility of the study. This inquiry was conducted by experts of the Natural History Research Center and Museum Baghdad University. A report with the findings was published on 12 April 2016. The report is available at https: osf.io ajsvw Below a preliminary summary of the main findings of this report. (The report is written in the Arab language.) * the experts conclude that Al-Sheikhly *et al*. (2013) contains inaccurate and wrong conclusions. * the experts conclude that the study reported in Al-Sheikhly *et al*. (2013) is in general weak in providing evidences. * the experts conclude that the research reported in Al-Sheikhly *et al*. (2013) is not convincing. * the experts conclude that Al-Sheikhly *et al*. (2013) contains conclusions which are not supported by field data. * the experts state that Al-Sheikhly *et al*. (2013) contains incorrect information about the occurrence of the species in western Iraq. * the experts support the statements in Porter *et al*. (2015a b) that male and females of the Basra Reed Warbler cannot be identified in the field merely based on seeing them. * the experts support the other methodological issues which are listed in Porter *et al*. (2015a). * the experts propose that all unpublished data will become available. * the experts support the preliminary investigations which have been published on 1 July 2016 as the report *Final investigation on serious allegations of fabricated and or falsified data in Al-Sheikhly et al. (2013, 2015) - 1 July 2016* (available at https: osf.io j69ue ). * the experts were not satisfied with the responses in Al-Sheikhly *et al*. (2015), in particular in regard to the issues mentioned in their own report and to the issues mentioned in the preliminary investigations (which were published on 1 July 2016, see above). * the experts propose to ask the opinion of external experts with thorough knowledge on reed warblers (*Acrocephalus* ssp.). Such an opinion is listed in the report at https: osf.io j69ue Comments on this preliminary summary of the main findings of the report by the experts of the Natural History Research Center and Museum Baghdad University are highly appreciated. Porter *et al*. (2015a): https: www.tandfonline.com doi full 10.1080 09397140.2015.1023424 Porter *et al*. (2015b): https: www.tandfonline.com doi full 10.1080 09397140.2015.1023426 , 
 It is encouraging that the authoritative and frequently used source http: datazone.birdlife.org species factsheet Basra-Reed-Warbler does not refer to this study. It is also encouraging that a recent paper on the first record of this species in Turkey http: journals.tubitak.gov.tr zoology issues zoo-19-43-2 zoo-43-2-13-1808-16.pdf (doi: 10.3906 zoo-1808-16 ) does not refer to this study. Publisher TF has until now been unable to provide a single review comment from an expert from which it can be concluded that this study is based on solid science. TF has until now also been unable to provide a single name of an expert who simply states that this study is based on solid science. There is until now no progress in the efforts to get access to the raw research data of this paper. , 
 A new comment at https: www.researchgate.net project Retracting-fraudulent-articles-on-the-breeding-biology-of-the-Basra-Reed-Warbler-Acrocephalus-griseldis states that the NGO BirdLife South Africa https: www.birdlife.org.za is urging publisher TF to retract this study. , 
 See also https: osf.io 6m3cq (published online on 14 February 2020). , 
 See https: doi.org 10.22541 au.160227182.23124117 v1 ,