Swainsonine protects H9c2 cells against lipopolysaccharide-induced apoptosis and inflammatory injury via down-regulating miR-429
Authors: Hongyan Lv
Xiaohong Hao
Su Zhang
Journal: Cell Cycle (2020)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Pediatric myocarditis (PM); lipopolysaccharide (LPS); miR-429; p38MAPK/NF-κB pathway; swainsonine (SW).
Affiliations: Department of Pediatrics, Jining No.1 People's Hospital, Jining, China., Department of Nursing, Jining No.1 People's Hospital, Jining, China.

Abstract:  Pediatric myocarditis (PM) is usually related to myocardial dysfunction. Generally, 30% of PM patients will die or undergo heart transplantation. Swainsonine (SW) is a natural alkaloid and an anti-cancer substance. Our goal was to determine the roles of SW in PM in current study. H9c2 cells were pre-treated by lipopolysaccharide (LPS). Viability and apoptosis were evaluated utilizing CCK-8 assay and flow cytometry. Inflammatory cytokines' mRNA expression and production were assessed by western blot and ELISA. Western blot was utilized to distinguish apoptosis and immune-related factors expression. Sequentially, the abovementioned parameters were reassessed when miR-429 was overexpressed. LPS declined viability as well as raised apoptosis and inflammatory injury in H9c2 cells. SW alleviated apoptosis and inflammatory injury induced by LPS. MiR-429 expression was elevated by LPS and suppressed by SW. SW-induced the increasing of viability and the reduction of inflammatory injury were reversed by overexpression of miR-429. Eventually, SW inhibited p38MAPK/NF-κB pathway which activated by LPS via overexpressing miR-429. SW exerted its anti-apoptosis and anti-inflammatory function in LPS-treated H9c2 cells through p38MAPK/NF-κB pathway and down-regulation of miR-429. 

Retracted: No

PubPeer comments:
 Fig. 7A.  LINC00963 inhibited related protein expressions in the FoxO signaling pathway detected by western blot assay Notes: A, protein bands for protein expressions in each group . ![file] The bands (and specifically the loading control) appear to be drawn from a small concourse of WBs. Here is a montage I prepared earlier. ![] Clockwise from upper left: Fig 6B,  protein band graph , from  MicroRNA-374 Exerts Protective Effects by Inhibiting SP1 Through Activating the PI3K Akt Pathway in Rat Models of Myocardial Ischemia-Reperfusion After Sevoflurane Preconditioning [(Zhang et al 2018)] . Fig 4D,  GLP-1R and CREB proteins in the myocardium of mice in each group examined by western blotting , from  Suppression of Long Non-Coding RNA LINC00652 Restores Sevoflurane-Induced Cardioprotection Against Myocardial Ischemia-Reperfusion Injury by Targeting GLP-1R Through the cAMP PKA Pathway in Mice [(Zhang et al 2018)] . Fig. 6B,  ELISA and western blot analysis were applied to determine the miR- 495 expression and mRNA and protein expression of NLRP3, TNF-u03b1, IL-1u03b2, IL-18 and caspase-1 , from  MicroRNA-495 Ameliorates Cardiac Microvascular Endothelial Cell Injury and Inflammatory Reaction by Suppressing the NLRP3 Inflammasome Signaling Pathway [(Zhou et al 2018)] . Fig 6B,  WB images showing protein expression of different genes in each group , from  MicroRNA-27a Suppresses Detrusor Fibrosis in Streptozotocin-Induced Diabetic Rats by Targeting PRKAA2 Through the TGF-u03b21 Smad3 Signaling Pathway . [(Wu et al 2018)] . Fig 11E,  protein bands of each factor in ASMCs of asthma mice in each group detected by western blot analysis , from  MicroRNA-485 Modulates the TGF-u03b2 Smads Signaling Pathway in Chronic Asthmatic Mice by Targeting Smurf2 . [(Wang et al 2018)] . , 
 [Expression of Concern] The former and current Publishers and Editor are jointly issuing an Expression of Concern on the article entitled u201cEffects of Long Non-Coding RNA LINC00963 on Renal Interstitial Fibrosis and Oxidative Stress of Rats with Chronic Renal Failure via the Foxo Signaling Pathwayu201d [Cell Physiol Biochem 2018;46:815-828;DOI: 10.1159 000488739] by Wen Chen, Lei Zhang, Zhong-Qi Zhou, Yue-Qin Ren, Li-Na Sun, Yu-Lin Man, Zhong-Wei Ma and Zhi-Kui Wang. After publication of this article, concerns were raised about the integrity of some of the data presented in the article. The authors were unresponsive to our correspondence within the given timeframe. The matter was raised to the research management officers at the corresponding author s institution who have confirmed the launch of an internal investigation into the concerns. Until the conclusion of this investigation, readers should interpret the data presented in the article with caution. Appropriate editorial action will be taken once the investigation is concluded. ,