Suppression of adriamycin resistance in osteosarcoma by blocking Wnt/β-catenin signal pathway
Authors: Z-G Bi
B-Q Wu
Y Cao
Journal: European Review for Medical and Pharmacological Sciences (2017)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: Wnt/β-catenin signal pathway; B-cell lymphoma 2 (Bcl-2); drug resistance; osteosarcoma cells; cell proliferation
Affiliations: Department of Orthopedics, First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. .

Abstract:  Objective: Wnt/β-catenin signal pathway plays a role in regulating cell proliferation and apoptosis, and is correlated with tumor onset, progression and drug resistance. B-cell lymphoma 2 (Bcl-2) is an anti-apoptotic factor inducing tumor cell drug resistance. Wnt/β-catenin signal pathway can modulate Bcl-2 expression. This study established a cell model of drug resistance using adriamycin (ADM) treatment. Wnt/β-catenin signal pathway was intervened to discuss its role in drug resistance of osteosarcoma cells. Materials and methods: Expression of β-catenin and Bcl-2 was compared between U2OS and hFOB1.19 cells. ADM resistant cell line U2OS/ADM was established for comparing β-catenin and Bcl-2 expression. Cell counting kit-8 (CCK-8) assay was used to test cell proliferation, followed by flow cytometry for apoptotic rate under ADM concentrations. U2OS/ADM cells were further treated with si-β-catenin and/or β-catenin inhibitor XAV939. β-catenin and Bcl-2 expression were measured, followed by CCK-8 and flow cytometry. Results: Comparing to hFOB1.19 cells, U2OS cells had significantly elevated β-catenin and Bcl-2 expression. U2OS/ADM cells had higher β-catenin and Bcl-2 expression than U2OS, plus lower ADM sensitivity and suppressed apoptotic rate. Transfection of si-β-catenin and XAV939 suppressed β-catenin and Bcl-2 expression, and significantly enhanced ADM sensitivity and ADM-induced apoptosis. Conclusions: Up-regulation of β-catenin plays a role in potentiating expression and downstream anti-apoptotic factor Bcl-2, and in enhancing ADM resistance of osteosarcoma U2OS cells. 

Retracted: Yes

PubPeer comments:
 Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cSuppression of adriamycin resistance in osteosarcoma by blocking Wnt u03b2-catenin signal pathway, by B.-Q. Wu, Y. Cao, Z.-G. Bi, published in Eur Rev Med Pharmacol Sci 2017; 21 (14): 3185-3192u2013PMID: 28770967u201d has been withdrawn. [The Publisher apologizes for any inconvenience this may cause] . ![file] The paper had been flagged in a Google document as showing [the signs of a papermill provenance] . https: docs.google.com spreadsheets d 1qBqQMXgzxoBd5bTbqdMuapGheupE5bdIopypju417NM edit#gid=0 One common feature of this particular papermill is a disconnection between the name of the nominal author and the email address provided for contact. ![file] , 
 Via http: www.most.gov.cn zxgz kycxjs kycxgzdt 202203 t20220318_179892.html u3000u30004. The paper  Suppression of adriamycin resistance in osteosarcoma by blocking Wnt u03b2-catenin signal pathway in the First Affiliated Hospital of Harbin Medical University, with Cao Yang as the corresponding author and Wu Binqi as the first author. After investigation, it is found that the paper has the behavior of writing and investing, buying and selling papers. Wu Binqi was dealt with as follows: the scientific research integrity admonition talk, the cancellation of the application for scientific and technological plans (special projects, funds, etc.) projects and awards for 5 years, the cancellation of the qualification for promotion of postgraduate tutors for 4 years, and the cancellation of the qualification for promotion of professional titles for 3 years; Cao Yang was dealt with as follows: The scientific research integrity exhortation talk, the cancellation of the application for scientific and technological plans (special projects, funds, etc.) projects and awards for 3 years, and the cancellation of the admission qualification for academic degree graduate students for 3 years. ,