Downregulation of NOB1 suppresses the proliferation and tumor growth of non-small cell lung cancer in vitro and in vivo
Authors: Donghua Qian
Yang Li
Hongyu Jing
Chengyuan Ma
Ming Qian
Zhongmei Wen
Journal: Oncology Reports (2014)
Discipline: CLINICAL MEDICINE
Keywords: Non-small cell lung cancer; NSCLC; diagnostic biomarkers; therapeutic targets; molecular targets
Affiliations: Department of Respiration, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China., Department of Neurosurgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China., Prosthodontics Department of the Stomatological Hospital, Jilin University, Changchun, Jilin 130021, P.R. China.

Abstract:  Non-small cell lung cancer (NSCLC) is a lethal disease due to the absence of effective diagnostic biomarkers and therapeutic targets. Therefore, novel molecular targets are critically needed to formulate new approaches for this devastating disease. In the present study, using quantitive real-time PCR and immunohistochemistry. we initially found that expression of the ribosome assembly factor NIN/RPN12 binding protein (NOB1) was elevated in the majority of NSCLC tissues when compared to that in the normal lung tissue counterparts, and its expression level was correlated with key pathological characteristics including tumor differentiation, stage and metastasis. Then, the recombinant lentiviral shRNA expression vector carrying NOB1 was constructed and infected into the human NSCLC A549 cell line. Cell proliferation, cell apoptosis, cell cycle distribution and colony formation ability in A549 cells were assessed following downregulation of NOB1 by siRNA. In addition, tumor growth ability in nude mice was evaluated to define the function of NOB1 in cell transformation and tumorigenesis. It was found that downregulation of NOB1 expression using the RNA silencing approach in A549 tumor cells significantly suppressed the proliferation and colony formation ability, and induced tumor apoptosis in vitro. Tumor growth was also suppressed in vivo. These data suggest that NOB1 is an important regulator of the tumorigenic properties of human NSCLC and may be used as a new promising diagnostic biomarker and a potential anticancer therapeutic target for NSCLC. 

Retracted: Yes

PubPeer comments:
 Concerns about Figure 4A: Some of the mice share unexpected similarities with each other. None of the mice are completely identical but certain features (shadows, colors in abdomen) are more similar than expected. ![file] , 
 2020 retraction. https: www.spandidos-publications.com 10.3892 or.2020.7483 Oncol Rep 31: [Related article:] 1271u20111276, 2014; DOI: 10.3892 or.2014.2991. The authors wish to retract their article entitled u2018Downu00adregulation of NOB1 suppresses the proliferation and tumor growth of nonu2011small cell lung cancer in vitro and in vivou2019, published in Oncology Reports 31: 1271u20111276, 2014. The authors have identified that the results shown in Fig. 4A did not display a significant level of difference comparing among the groups, which undermines the conclusions stated in the article. In addition, the u2018Acknowledgementsu2019 section featured an error in terms of the quoted project number. For these reasons, the authors have decided to withdraw this paper from the Journal. All the named authors agree to this retraction. and regret any inconvenience to the readers and to the Editor of Oncology Reports that this retraction will cause. ,