RETRACTED ARTICLE: Acute Toxicity and Gastroprotection Studies of a New Schiff Base Derived Manganese (II) Complex against HCl/Ethanol-Induced Gastric Ulcerations in Rats
Authors: Mazen M Jamil Al-Obaidi
Summaya M Dhiyaaldeen
Najihah Mohd Hashim
Mohamed Yousif Ibrahim
Hoyam Adam
Hapipah Mohd Ali
Rashd M El-Ferjani
Bassam Alkotaini
Rami Al Batran
Journal: Scientific Reports (2016)
Discipline: Multidisciplinary
Keywords: Manganese; gastroprotective efficacy; Mn (II) complex; gastric ulceration; rats
Affiliations: Department of Pharmacy, Faculty of Medicine, University of Malaya 50603 Kuala Lumpur, Malaysia., Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia., Center for Natural Products and Drug Discovery (CENAR), University of Malaya, Kuala Lumpur, Malaysia., Department of Microbiology, Faculty of medicine, University of Duhok, 78 Kurdistan, Iraq., Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia., Department of Chemistry, Faculty of Science, University of Benghazi, 1308 Benghazi, Libya., School of Pharmacy, Ahfad University for Women (AUW), 167 Omdurman, Sudan., Department of Chemical Engineering, Faculty of Engineering, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea., Institute of Research Management &Monitoring, Deputy Vice Chancellor (Research &Innovation), University of Malaya, 50603 Kuala Lumpur, Malaysia.

Abstract:  Manganese is a crucial element for health. In this study, the gastroprotective efficacy of Mn (II) complex (MDLA) against acidified ethanol (HCl/Ethanol)-induced gastric ulceration in rats was evaluated. The animals were distributed into 5 groups. Groups 1 and 2 received carboxymethylcellulose (CMC), group 3 was pretreated with omeprazole, and groups 4 and 5 were given 10 and 20 mg/kg of MDLA, respectively. After one hour, CMC and HCl/Ethanol were given to groups 2-5 whilst the animals in group 1 were ingested with CMC. After sacrifice, gastric lesions were evaluated by wall mucus, gross appearance, histology, antioxidant enzymes and immunohistochemistry. Group 2 displayed severe gastric damage with a significant reduction in wall mucus. Conversely, gastric lesions were reduced in groups 3-5 by 85.72%, 56.51% and 65.93%, respectively. The rats in groups 3-5 showed up-regulation of heat shock protein 70 (Hsp70) with down-regulation of Bcl-2-associated protein x (Bax). Pretreatment with omeprazole or MDLA led to an increase in the uptake of Periodic Acid Schiff (PAS) stain in the glandular part of the gastric tissue, raised levels of prostaglandin E2 (PGE2) and superoxide dismutase (SOD), and a reduction in malondialdehyde (MDA) concentrations. These results suggested the gastroprotective action of Mn (II) complex. 

Retracted: Yes

PubPeer comments:
 2020 retraction. https: www.nature.com articles s41598-020-63217-y Retraction of: Scientific Reports https: doi.org 10.1038 srep26819, published online 27 May 2016 The Editors have retracted this Article. An investigation at the University of Malaya identified figure assembly and data integrity issues. Specifically, the following images were duplicated from a previous publication [1]: Fig. 6 G3 (Hsp70 stain, omeprazole (20mg kg)) and Fig. 6 G4 (Hsp70 stain, MDLA (500mg kg)) were duplicates of Fig. 9E (HSP70 stain, omeprazole (20mg kg)) and Fig. 9C (HSP70 stain, HPTP (50mg kg)), respectively. These issues undermine confidence in the study which cannot be considered reliable. Najihah Mohd Hashim, Mazen Al-Obaidi, Bassam Alkotaini, and Rami Al Batran agree with the retraction. The other authors did not respond to correspondence about this retraction. Reference 1. Dhiyaaldeen, S. M. et al. Protective effects of (1-(4-hydroxy-phenyl)-3-m-tolyl-propenone chalcone in indomethacin-induced gastric erosive damage in rats. BMC Vet Res 10, 961 (2014). ,