LncRNA H19 secreted by umbilical cord blood mesenchymal stem cells through microRNA-29a-3p/FOS axis for central sensitization of pain in advanced osteoarthritis
Authors: Yan Li
Guan Wang
Xiaolun Huang
Guangming Xiang
Yutong Yao
Daqiang Zhao
Maozhu Yang
Haibo Zou
Chunyou Lai
Le Luo
Qinyan Yang
Ying Shi
Journal: American journal of translational research (2021)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: LncRNA H19/microRNA-29a-3p/FOS axis; Pain of advanced osteoarthritis; central sensitization; umbilical cord blood mesenchymal stem cells.
Affiliations: Department of Hepatobiliary and Pancreatic Surgery, Cell Transplantation Center, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Chengdu 610072, China., Department of Anesthesiology, Shanghai Jiahui International Hospital Shanghai 200233, China., School of Medicine, University of Electronic Science and Technology of China Chengdu 610054, Sichuan, China.

Abstract:  Objective: To explore the molecular mechanism of umbilical cord blood mesenchymal stem cells (UCBMSCs) in the treatment of advanced osteoarthritis pain. Methods: Normal healthy rats were selected to establish advanced osteoarthritis (OA) model, and the rats were randomly divided into control group, intravenous group, intracavitary group and intrathecal group. The intravenous group received intravenous injection of UCBMSCs, intracavitary group received intra-articular injection of UCBMSCs, and intrathecal group received subarachnoid injection of UCBMSCs. The pain behavior and serum pro-inflammatory factor levels were evaluated before and after treatment. microRNA-29a-3p and FOS mRNA in spinal dorsal horn was detected using qPCR, the phosphorylation of c-fos protein and NR1, NR2B, ERK and PKCg was detected using Western blot, and the level of LncRNA H19 was detected using qPCR. Results: LncRNA H19 was enriched in the exosomes of UCBMSCs. microRNA-29a-3p was the target gene of LncRNA H19, while FOS was the downstream target of microRNA-29a-3p. Pain and inflammation of rats in the intrathecal group improved best, and the phosphorylation levels of c-fos and NR1, NR2B, ERK and PKCg in the spinal dorsal horn of the intrathecal group decreased. LncRNA H19 regulated the central sensitization of astrocytes through microRNA-29a-3p/FOS axis. Conclusion: Intrathecal injection of umbilical cord blood mesenchymal stem cells can improve the pain and central sensitization of advanced osteoarthritis through LncRNA H19/microRNA-29a-3p/FOS axis. 

Retracted: No

PubPeer comments:
 Figure 11. ![file] By the way, Jiang is a full-time researcher. , 
 Thank you for pointing out the problem in our paper on Figure 11. We felt very sorry for the occurrence of these mistakes. Due to the large number of pictures, mistakes occurred when combining the hearts and liversu2019 images. However, we found no problem with the spleen in the MSC group. We have contacted the journal and made corrections. Thank you again for your concerns on our study. , 
 The TEM image in Figure 2A in International Journal of Nanomedicine (2019) is part of the TEM image in Figure 2A in Biomaterials Science (2018) ![file] , 
 #3 Thank you very much for your comments. On August 3, 2020, we have corrected Figure 2 and Figure 11 together. , 
 The authors have advised the scale bars of the transmission electron microscope shown in Figure 2A and C on page 578 is incorrect. The incorrect image for Figure 2A was also used. The correct Figure 2 is shown. The authors have also advised when preparing the images for Figure 11A on page 587, the incorrect images were used for the heart Normal and PBS groups and the liver PBS and MSC+Fe3O4 groups. This inadvertently led to the duplication of these images. The correct Figure 11 is shown. ![file] ![file] , 
 Figure 8 of 10.2147 ijn.s258017 (Original August 2020) appears to include an image with **different description, rotation, dimensions, and coloring**, that seems to overlap with an image included as a correction to 10.2147 ijn.s184920 (Original Jan 2019), that was published as 10.2147 IJN.S275285 (Correction Aug 2020), which is shown in #5 above. All of this occurred in the same journal. As the dates are similar, I am posting to both PubPeer threads. *** ![file] , 
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