Retracted : Circular RNA circC3P1 restrains kidney cancer cell activity by regulating miR‐21/PTEN axis and inactivating PI3K/AKT and NF‐ k B pathways
Authors: Lei Xin
Tao Chen
Lei Guo
Qinchao Yu
Journal: Journal of Cellular Physiology (2020)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: circC3P1; growth; invasion; kidney cancer; miR-21
Affiliations: Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

Abstract:  Kidney cancer (KC) seriously impacts public health. We detected the function and mechanism of circular RNA C3P1 (circC3P1) in KC cells. CCK-8, flow cytometry, migration, and invasion assay were respectively used to investigate the efficacies of circC3P1 and microRNA (miR)-21 on cell viability, apoptosis, migration, and invasion. Phosphatase and tensin homologue deleted on chromosome 10 (PTEN), circC3P1, and miR-21 expression were changed by cell transfection and detected by quantitative reverse-transcription polymerase chain reaction. Moreover, the apoptosis/pathways-related proteins and proteins were detected by western blot analysis. Besides, the relation between PTEN and miR-21 was detected by luciferase assay. circC3P1 and PTEN were downregulated while miR-21 was upregulated in KC tissues. circC3P1 declined cell viability, migration, and invasion and caused apoptosis. Furthermore, circC3P1 negatively regulated miR-21; miR-21 mimic could reverse the efficacies of circC3P1. Besides, circC3P1 restrained the PI3K/AKT and NF-κB pathways by downregulating miR-21. Finally, PTEN was authenticated as a target of miR-21. circC3P1 restrained KC cell growth, migration, and invasion by regulating miR-21/PTEN axis and inactivating PI3K/AKT and NF-κB signaling pathways. 

Retracted: Yes

PubPeer comments:
 The Western blots and other figures in this paper have an interesting layout that appears to be shared with many other papers, as discussed in a January 2020 post on Leonid Schneider s blog: https: forbetterscience.com 2020 01 24 the-full-service-paper-mill-and-its-chinese-customers The list of these papers is available here: https: docs.google.com spreadsheets d 1KXqTAyl4j-jVorFPMD2XRpr76LcIKJ0CVyIvRj0exYQ edit?usp=sharing In almost all papers, Western blot panels within the same figure, and across figures and papers, appear to share the same background, while the bands are regularly spaced, all have similar rounded edges without the usual smudges and specks, and with some bands showing a recognizable  jumping sardine shape. Also in almost all papers, bar graphs show a characteristic layout with bars in solid shades of grey, and the left-most bar in black, as well as double-edged error bars. Many papers have  cell viability and  apoptotic cells graphs. To illustrate the recognizable layout of the graphs in this paper, here is part of Figure 3. ![file] , 
 2021 retraction. https: onlinelibrary.wiley.com doi 10.1002 jcp.30438 Retraction: u201cCircular RNA circC3P1 restrains kidney cancer cell activity by regulating miR-21 PTEN axis and inactivating PI3K AKT and NF- kB pathways,u201d by Tao Chen, Qinchao Yu, Lei Xin, Lei Guo, J Cell Physiol. 2020; 4001-4010: The above article, published online on 23 October 2019 in Wiley Online Library , has been retracted by agreement between the journal s Editor in Chief, Prof. Dr. Gregg Fields, and Wiley Periodicals LLC. The retraction has been agreed following an investigation based on allegations raised by a third party. Several flaws and inconsistencies between results presented and experimental methods described were found, the editors consider the conclusions of this article to be invalid. The authors collaborated in the investigation initially, but were not available for a final confirmation of the retraction. ,