Long Noncoding RNA Plasmacytoma Variant Translocation 1 (PVT1) Promotes Colon Cancer Progression via Endogenous Sponging miR-26b
Authors: Jianfeng Zhao
Jibin Li
Xin Liu
Keer Jin
Wenya Li
Yefu Liu
Xiaofei Yan
Wen Shang
Rui Zhang
Journal: Medical Science Monitor (2018)
Discipline: CLINICAL MEDICINE
Keywords: lncRNA; PVT1; colon cancer; miR-26b; tumor progression
Affiliations: Department of Colorectal Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning, China (mainland)., Department of Colorectal Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning, Cambodia., Department of Hepatopancreatobiliary Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning, China (mainland).

Abstract:  BACKGROUND Recently, long noncoding RNAs (lncRNAs) have received wide attention in the area of tumor progression. Dysregulation of lncRNAs has been shown to participated in colon cancer, a known malignant tumor. This study aimed to identify the way lncRNA PVT1 affects the progression of colon cancer. MATERIAL AND METHODS Both human colon cancer tissues and 30 paired adjacent normal tissue samples, as well as the colon cancer cells, were collected. Then quantitative real-time (qRT-PCR) was performed to detect the expression of lncRNA PVT1 and miR-26b. Furthermore, the role of PVT1 was determined by function assays such as cell proliferation assay, invasion assay, and wound healing assay. The mechanism was studied using western blot assay and luciferase assay. RESULTS We demonstrate that the expression of PVT1 was significantly higher in tumor tissue compared with the adjacent normal tissue with a lower expression of miR-26b. Moreover, PVT1 promoted tumor growth, migration, and invasion in vitro. In addition, further experiments revealed that miR-26b was a direct target of PVT1 and could inhibit cell migration, invasion, and proliferation in colon cancer. CONCLUSIONS Our results suggest that PVT1 could promote metastasis and proliferation of colon cancer via endogenous sponging and inhibiting the expression of miR-26b, which may highlight the significance of lncRNA PVT1 in colon cancer tumorigenesis. 

Retracted: No

PubPeer comments:
 Fig 3A.  LncRNA PVT1 promotes colon cell migration, invasion, and proliferation. (A, B) Wound healing assays were performed with cells transfected with either pWPXL-PVT1 or si-PVT1 to reveal the relative mobility. Two 0-hour panels are the same, apart from different lines quantifying the initial scratch width. ![file] , 
 Fig 5A.  MiR-26b inhibits cell migration, invasion and proliferation in CC. (A, B) The effects on cell migration in HCT116 cells after transfection with miR-26b mimics and inhibitors . ![file] Two panels overlap (with a rotation). , 
 Fig 3C.  Twenty-four hours post-transfection, cell invasion was evaluated in HCT116 cells using a Transwell system with 8 u03bcm pores in polycarbonate membranes. Representative views are shown. ![file] For comparison: Fig 3C from [Meng et al (2019)] .  The effect of pWPXL-SNHG6 or si-SNHG6 on the invasion of HCT-116 cells was determined using Transwell invasion assays . ![file] , 
 Retracted [6 August 2021] .  Retracted on authorsu2019 request due to detected data flaws . ,