MicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcoma
Authors: Ren-Tao Zhao
Xiao Li
Yang Chen
Li Chen
Fan-Bin Meng
Wei Wang
Journal: Cytogenetic and Genome Research (2015)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: miR-150; osteosarcoma; cell proliferation; invasion; apoptosis
Affiliations: Department of Bone Tumor, Linyi People's Hospital, Linyi, PR China.

Abstract:  miR-150 expression in osteosarcoma (OS) cell lines and human osteoblast cells was detected, and OS cell models were transfected with exogenous miR-150 to investigate its role in cell proliferation, invasion, and apoptosis. Our results showed that miR-150 expression in OS cells (MG63, Saos-2, SOSP-9607, and U2OS) was significantly lower compared to the osteoblast hFOB1.19 cell line (all p &lt; 0.01). The expression level of miR-150 in MG63 cells that were transfected with exogenous miR-150 mimics was markedly upregulated, while the miR-150 expression level in the inhibitor group was significantly downregulated (both p &lt; 0.01). Similar results were also found in SOSP-9607 cells. Importantly, exogenous miR-150 expression stimulated cell apoptosis and inhibited proliferation, invasion, and migration. A luciferase reporter assay displayed that miR-150 also regulated Sp1 expression by targeting its 3'-UTR, and qRT-PCR and Western blotting showed that elevated levels of miR-150 may reduce Sp1 protein expression. The mRNA and protein levels of Sp1 were upregulated after transfection with a Sp1-expression plasmid and partially reversed the inhibitory effects of miR-150 on cell proliferation, invasion, and metastasis in MG63 and SOSP-9607 cells, as well as promoted cell apoptosis. In conclusion, miR-150 inhibits cell proliferation, invasion, and metastasis and stimulates cell apoptosis by regulating the expression of Sp1. Therefore, miR-150 may be a potential clinical target for the treatment of OS patients. 

Retracted: Yes

PubPeer comments:
 There appear to be image overlaps between figures 6 and 11: ![file] ![file] , 
 Fig 4.  Flow cytometry analysis showing the effect of miR-150 on stimulating MG63 and SOSP-9607 cell apoptosis. The apoptosis rates of MG63 and SOSP-9607 cells were significantly higher in the miR-150 group than in the blank group, but apoptosis rates of MG63 and SOSP-9607 cells were markedly lower in the inhibitor group than in the blank group. ![file] For comparison, Figs 3A and 3C from [Wang et al (2019)] : ![] ![] , 
 Fig. 13. miR-150 stimulates cell apoptosis by regulating the protein expression of Sp1 in both MG63 and SOSP- 9607 cells. ![file] I have not checked other panels for fear of calling down the wrath of the Society for the Prevention of Cruelty to Dead Horses. , 
 Dendrodoa Grossularia has elsewhere pointed out an overlap between the present Figs 6, 8, 11 *[at left]* and *[at right]* Figs 8 and 9 from [Qiao et al (2017)] ![] Qiao et al has figure overlaps with a series of other papers, including [Xie et al (2017)] and [Ba et al (2017)] ... ![] ...with the corollary that versions of the other papers figures can also be found here. , 
 #4 Here, Fig 5B from Xie et al (2017)... ![file] ...and the present Figs 6 and 11. ![file] , 
 It is difficult to show all the connections among [Bai et al (2016)] (Fig 4A), [Li et al (2016)] (Figs 7 and 12), and [He et al (2016)] (Figs 13 and 14) without running rapidly out of colors. I have glossed over the 90-deg or 180-deg rotations involved in some of these correspondences. ![file] , 
 At right, Figs 7 and 12. At the left: Figs 5 and 6 from [Zhao et al (2016)] . With Figure 5A of [Guo et al (2018)] at bottom left. ![file] , 
 #2 Another sighting: Fig 3A of [Chen et al (2016)] . ![] And another! Fig. 3 of [Gao et al (2018)] .  Effects of OP-B on the apoptosis of HT-29 and HCT-116 cells. ... The effect of different concentrations of OP-B (0, 5, 10, and 20 u03bcmol l) on the apoptosis of HT-29 (A) and HCT-116 (B) colon cancer cells detected by flow cytometry . ![file] , 
 Expression of Concern, [26 July 2021] . ---------------------------- The Publisher and Editors are jointly issuing an Expression of Concern on the article entitled u201cMicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcomau201d [Cytogenet Genome Res. 2015;146:124u2013135, DOI: 10.1159 000437379] by Xiao Li, Li Chen, Wei Wang, Fan-Bin Meng, Ren-Tao Zhao, and Yang Chen. After publication of this article, concerns were raised about the integrity of some of the data presented in the article. The authors were unresponsive to our correspondence within the given timeframe. The matter was raised to the research management officers at the corresponding authoru2019s institution who have confirmed the launch of an internal investigation into the concerns. Until the conclusion of this investigation, readers should interpret the data presented in the article with caution. Appropriate editorial action will be taken once the investigation is concluded. , 
 Retracted [1 March 2022] . -------------------------------- The article u201cMicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcomau201d [Cytogenet Genome Res. 2015;146:124u201335. doi: 10.1159 000437379] by Xiao Li, Li Chen, Wei Wang, Fan-Bin Meng, Ren-Tao Zhao, and Yang Chen has been retracted by the Publisher and the Editors. After the publication of this article, concerns were raised about the integrity of some of the data presented in the article. The authors were unresponsive to our correspondence within the given timeframe. The matter was raised to the research management officers at the corresponding authoru2019s institution who confirmed the launch of an internal investigation into the concerns. An Expression of Concern was published: https: doi.org 10.1159 000518607. The research management officers informed the journal that their investigation found issues in the article and that the article should be retracted. The article is being retracted based on the stated findings of the institution. The authorsu2019 institution did not respond to our request to provide more detail about the results of the investigation and the authors have not responded to our correspondence regarding this retraction despite multiple contact attempts. ,