RETRACTED ARTICLE: A Chinese herbal formula, Jian-Pi-Yi-Shen decoction, improves muscle atrophy via regulating mitochondrial quality control process in 5/6 nephrectomised rats
Authors: Xinhui Liu
Jianping Chen
Shunmin Li
Ping Zheng
Tiegang Yi
Dongtao Wang
Gaofeng Song
Journal: Scientific Reports (2017)
Discipline: Multidisciplinary
Keywords: Muscle atrophy; Chronic kidney disease; Mitochondrial quality control; Jian-Pi-Yi-Shen decoction; Mitochondrial biogenesis
Affiliations: Department of Nephrology, Shenzhen Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen, 518033, China. ., Department of Nephrology, Ruikang Affiliated Hospital, Guangxi University of Chinese Medicine, Nanning, 530011, China. ., Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen, 518033, China., Department of Nephrology, Shenzhen Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen, 518033, China., Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen, 518033, China. .

Abstract:  Muscle atrophy is one of the serious complications of chronic kidney disease (CKD). Dysregulation of mitochondrial quality control (MQC) process, including decrease mitochondrial biogenesis, impair mitochondrial dynamics and induce activation of mitophagy, play an important role in mediating muscle wasting. This study aimed to observe effects of Jian-Pi-Yi-Shen (JPYS) decoction on muscle atrophy in CKD rats and explore its possible mechanism on regulation of MQC processes. The 5/6 nephrectomised rats were randomly allocated into 2 groups: CKD group and JPYS group. Besides, a sham-operated rats as sham group. All rats were treated for 6 weeks. Results showed that administration of JPYS decoction prevented body weight loss, muscle loss, muscle fiber size decrease, muscle protein degradation, and increased muscle protein systhesis. In addition, JPYS decoction increased the mitochondrial content and biogenesis proteins, and down-regulated the autophagy and mitophagy proteins. Furthermore, JPYS decoction increased mitochondrial fusion proteins, while decreased mitochondrial fission proteins. In conclusion, JPYS decoction increased mitochondrial content and biogenesis, restore the balance between fission and fusion, and inhibited autophagy-lysosome pathway (mitophagy). Collectively, our data showed that JPYS decoction to be beneficial to muscle atrophy in CKD, which might be associated with the modulation of MQC process. 

Retracted: Yes

PubPeer comments:
 Two smears in Figure 3a appear unexpectedly similar when flipped horizons vertically. Could the authors please post the original uncropped scans? *** Contrast enhanced closeup ![file] *** Showing original orientation and contrast ![file] , 
 Dear Dr. Actinopolyspora Biskrensis, Thank you very much for pointing out the mistakes of Figure 3a in our published work. We have carefully investigated our raw data and found that the error was inadvertently made during the preparation of the manuscript. We will contact the journal editorial office as soon as possible to correct the error. It may take some times. Thank you again for your valuable comment and give us the opportunity to correct our mistake. Sincerely, Wang , 
 #2 Thank you for your prompt reply. Could you please post the original data here? Thank you. , 
 Image duplication in Figure 1 ![file] , 
 Bands in the red box are unexpectedly similar in figure 5 and figure 6. ![file] , 
 An additional overlap in Figure 4A. The type and quantity of u201cerrorsu201d in this paper do not inspire confidence. *** ![file] , 
 2021 retraction. https: www.nature.com articles s41598-021-91869-x The authors have retracted this article. After publication they became aware of a number of irregularities in the figures, specifically: In Figure 3a the Sham band appears to be identical for the Atrogin-1 and MuRF1 blots In Figure 1d the Sham and CKD panels appear to partially overlap In Figure 4a the Sham and JPYS panels appear to partially overlap Dongtao Wang, Jianping Chen, Xinhui Liu, and Shunmin Li agree to this retraction. Ping Zheng, Gaofeng Song, and Tiegang Yi did not respond to correspondence about this retraction. ,