Description of Gabonibacter massiliensis gen. nov., sp. nov., a New Member of the Family Porphyromonadaceae Isolated from the Human Gut Microbiota
Authors: Jean Bernard Lekana-Douki
Pierre-Edouard Fournier
Angélique Ndjoyi-Mbiguino
Nicholas Armstrong
Catherine Robert
Didier Raoult
Jaishriram Rathored
Gaël Mourembou
Saber Khelaifia
Journal: Current Microbiology (2016)
Discipline: MICROBIOLOGY
Keywords: Culturomics; Gabon; Gabonibacter massiliensis; Gut microbiota; Taxonogenomics.
Affiliations: URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée-Infection, Faculté de Médecine, Aix-Marseille Université, 27 Bd Jean Moulin, Marseille, France., Ecole Doctorale Régionale d'Afrique Centrale, B.P. 876, Franceville, Gabon., Unité de Parasitologie Médicale (UPARAM), CIRMF B.P. 769, Franceville, Gabon., Département de Parasitologie Mycologie et de Médecine Tropicale, Université des Sciences de la Santé, B.P. 4009, Libreville, Gabon., Département de Microbiologie, Laboratoire national de référence IST/sida, Faculté de Médecine, Université des Sciences de la Santé, B.P. 4009, Libreville, Gabon., Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia., URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée-Infection, Faculté de Médecine, Aix-Marseille Université, 27 Bd Jean Moulin, Marseille, France. .

Abstract:  The identification of human-associated bacteria is very important to control infectious diseases. In recent years, we diversified culture conditions in a strategy named culturomics, and isolated more than 100 new bacterial species and/or genera. Using this strategy, strain GM7, a strictly anaerobic gram-negative bacterium was recently isolated from a stool specimen of a healthy Gabonese patient. It is a motile coccobacillus without catalase and oxidase activities. The genome of Gabonibacter massiliensis is 3,397,022 bp long with 2880 ORFs and a G+C content of 42.09 %. Of the predicted genes, 2,819 are protein-coding genes, and 61 are RNAs. Strain GM7 differs from the closest genera within the family Porphyromonadaceae both genotypically and in shape and motility. Thus, we propose that strain GM7T (=CSUR P2336 = DSM 101039) is the type strain of the new genus Gabonibacter gen. nov. and the new species G. massiliensis gen. nov., sp. nov. 

Retracted: No

PubPeer comments:
 This paper is part of a set of 9 papers. According to the authors, all papers are related to the same protocol approved under the number 0023 2013 SG CNE by the National Ethics Committee of Gabon. Some of the authors are mentioned in the 9 papers. This protocol seems to cover 2 types of different objectives: -t3 papers describe a study on malaria in children. -t9 papers describe studies dedicated to characterize different new bacteria. **Study on malaria in children:** - 3 papers describe a study in hundreds of febrile and afebriles children. -tBlood samples are taken. -tThe study is performed between 2013 and 2014. **Studies to characterize different new bacteria:** 6 papers describe different protocols done on different participants. -tStools samples have been taken. No blood samples. -t4 papers describe a protocol performed on 1 child only. This is a different child for each paper. All samples have been taken on January 2015. -t1 paper describes a protocol performed on 1 adult only on January 2015 -t1 paper describes a protocol performed on 3 children in Gabon and Senegal in 2016. Can the authors explain how a unique protocol cover 2 different types of studies with 2 objectives so different? -tStudy designs are very different. -tRecruitment periods are different. -tTypes of participants are different -tTypes of sample are different, etcu2026 It seems pretty unusual that a same protocol cover -tblood samples in children to study malaria in 2013. -tand also a stool sample in 1 adult only, 2 years after. Can the authors explain the scope of the protocol Nu00b00023 2013 SG CNE and the approval process by the National Ethics Committee of Gabon for this 2 different type of design ? **Malaria in children u2013 Blood samples (2013-2014)** 1.t[Emerging infectious diseases - Possible role of Rickettsia felis in acute febrile illness among children in Gabon] 2.t[Malaria journal - Malaria in urban, semi-urban and rural areas of southern of Gabon: comparison of the Pfmdr 1 and Pfcrt genotypes from symptomatic children] 3.t[The American journal of tropical medicine and hygiene - Co-circulation of Plasmodium and bacterial DNAs in blood of febrile and afebrile children from urban and rural areas in Gabon] **Description of new bacteria u2013 Stool samples in children (or 1 adult)** 4.t[Current microbiology - Description of Gabonibacter massiliensis gen. nov., sp. nov., a new member of the family porphyromonadaceae isolated from the human gut microbiota] 5.t[New Microbes and New Infections - Taxonogenomics description of Bacillus dakarensis sp. nov., Bacillus sinesaloumensis sp. nov. and Bacillus massiliogabonensis sp. nov., three new species isolated from human stools] 6.t[New microbes and new infections - Noncontiguous finished genome sequence and description of Gabonia massiliensis gen. nov., sp. nov.] 7.t[New microbes and new infections - u201cIntestinimonas gabonensisu201d sp. nov., a new bacterium detected from a Gabonese stool specimen] 8.t[New microbes and new infections - Noncontiguous finished genome sequence and description of Kallipyga gabonensis sp. nov.] 9.t[New microbes and new infections - Discovery of a new species within Bacillus genus in a stool sample from Gabon:u201cBacillus massiliogabonensisu201d sp. nov.] ,