Effects of SDF-1/CXCR4 on the Repair of Traumatic Brain Injury in Rats by Mediating Bone Marrow Derived Mesenchymal Stem Cells
Authors: Xiao-Feng Xu
Jing Ren
Quan-Jun Deng
Journal: Cellular and Molecular Neurobiology (2018)
Discipline: NEUROSCIENCE & BEHAVIOR
Keywords: Bone mesenchymal stem cells and cell transplantation; CXCR4; SDF-1; Traumatic brain injury.
Affiliations: Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, 300052, People's Republic of China., Department of Clinical Laboratory, Fudan University Shanghai Cancer Center, Shanghai, 200032, People's Republic of China., Department of Oncology, Shanghai Medical College of Fudan University, Shanghai, 200032, People's Republic of China., Department of Medical Laboratory, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin, 300052, People's Republic of China. .

Abstract:  Our study aims to investigate the effects of the SDF-1/CXCR4 axis on the repair of traumatic brain injury (TBI) in rats by mediating bone marrow derived from mesenchymal stem cells (BMSCs). Healthy male SD rats were collected, their tibiofibulars were removed, cultured, and BMSCs were collected. The expression of cell-surface molecular proteins was examined using flow cytometry. The mRNA and protein expression of CXCR4 in cells were tested using qRT-PCR and western blotting analysis. An electronic brain injury instrument was utilized to build TBI rat models and each rat was assigned into the experiment, positive control and control groups (10 rats in each group). The morris water maze was used to calculate the escape latency and number of times rats in each group crossed the platform. Neurological severity scores (NSS) was calculated to evaluate the recovery of neurological functioning. The distribution of neuronal nuclear antigens was detected using double-labeling immunohistochemistry. The morphological changes in the hippocampal neuronal and the number of BrdU-positive cells were observed through Nissl's staining and high magnification. The mRNA and protein expressions of CXCR4 were gradually increased as SDF-1 concentration increased. NGF and BDNF positive cells were expressed in each group. The distribution of neuronal nuclear antigens in the experiment group was elevated compared to the control and positive control groups. Among the three groups, the experimental group had the shortest escape latency and the highest number platform crossings. The difference in NSS among the three groups was significant. The experimental group had better cell morphology and a higher number of BrdU-positive cells than the other groups. The present study demonstrates that transplanting BMSCs with SDF-1-induced CXCR4 expression can promote the repair of TBI. This is expected to become a new treatment regimen for TBI. 

Retracted: No

PubPeer comments:
 2020 retraction. https: www.nature.com articles s41598-020-63852-5 Retraction of: Scientific Reports https: doi.org 10.1038 srep15574, published online 27 October 2015 The authors are retracting this Article because of duplication of findings and figures from previously published studies [1,2] and issues with assembly of figures. Retraction Open Access Published: 23 April 2020 Retraction Note: Enhancement of the blue photoluminescence intensity for the porous silicon with HfO2 filling into microcavities Ran Jiang, Xianghao Du, Weideng Sun, Zuyin Han Zhengran Wu Scientific Reports volume 10, Article number: 7171 (2020) Cite this article 54 Accesses Metricsdetails The original article was published on 27 October 2015 Download PDF Retraction of: Scientific Reports https: doi.org 10.1038 srep15574, published online 27 October 2015 The authors are retracting this Article because of duplication of findings and figures from previously published studies [1,2] and issues with assembly of figures. Specifically, the production of porous-Si filled with HfO2, and the finding that HfO2-filled porous-Si enhances blue light emission have been reported by the authors in a previous publication [1], which was not cited in the Article. The inset in Fig. 2 is duplicated from Fig. 1b in [1], the spectra in Fig. 2 are previously published as Fig. 2a in [1], the data in Fig. 5 are published as Fig. 3 in [1], the information presented in Fig. 6 is published as Fig. 4 in [1], and the data in Fig. 7 have been presented in Fig. 2b of [1]. Additionally, Fig. 3b is duplicated from Fig. 1c in [2]. All Authors agree with this retraction. References 1. Jiang, R. et al. Strong photoluminescence of the porous silicon with HfO2-filled microcavities. Appl. Phys. Lett. 106, 252902, https: doi.org 10.1063 1.4922879 (2015). 2. Oakes, L. et al. Surface engineered porous silicon for stable, high performance electrochemical supercapacitors. Sci Rep 3, 3020, https: doi.org 10.1038 srep03020 (2013). ,