microRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric cancer
Authors: Gen Chen
Maohui Feng
Zhiliang Wang
Zhangshuo Yang
Journal: Bioscience reports (2020)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: EMT; PTPN14; gastric cancer; microRNA-217.
Affiliations: Gui Zhou Provincial People’s Hospital, Gui Yang, Gui Zhou, China, Department of Gastroenterological Surgery, Zhongnan Hospital, Wuhan University, Wuhan, China, Institute of Hepatobiliary Diseases, Transplant Center, Hubei Key Laboratory of Medical Technology on Transplantation, Zhongnan Hospital, Wuhan University, Wuhan, China, Zhongnan Hospital of Wuhan University, Wuhan, China

Abstract:  Background: Gastric cancer (GC) is the one of most common malignancies and its mechanism of metastasis remains unclear. The study was designed to investigate the effects of microRNA-217 on epithelial-to-mesenchymal transition. Methods: The expression levels of miR-217 in GC were assayed by real-time qPCR. Metastasis and invasion of cancer cell were assayed by transwell chamber. Double luciferase reporter gene was used to verify the target regulatory relationship between microRNA-217 and tyrosine-protein phosphatase non-receptor type 14 (PTPN14) on gastric cell lines. Epithelial-to-mesenchymal transition (EMT) markers were assayed by Western blot. Results: We found that miR-217 had a low level expression in gastric tumor tissues of 40 patients with GC, and a lower expression in the gastric tumor tissues of the patients with GC metastasis. Moreover, miR-217 markedly suppressed the metastasis and invasion of gastric cancer cell line in vitro. Furthermore, miR-217 inhibited the expression of PTPN14 by directly targeting its 3'UTR. Moreover, the down-regulation of PTPN14 reduced the metastasis and invasion, whereas up-regulation of PTPN14 led to the enhanced metastases and invasion of gastric cells. miR-217 induced the down-regulation of PTPN14 and inhibited the EMT in gastric cancer cells. Conclusion: miR-217 inhibited the EMT through directly targeting to the 3'UTR of PTPN14. 

Retracted: No

PubPeer comments:
 Papers seems to share same image. Curiously, they don t share any co-authors ![file] , 
 The Editorial Office has been made aware of potential issues surrounding the scientific validity of this paper, hence has issued an expression of concern to notify readers whilst the Editorial Office investigates. , 
 EXPRESSION OF CONCERN: MARCH 17 2022  This update follows the previous Expression of Concern for the article u201cmicroRNA-217 suppressed epithelial-to-mesenchymal transition through targeting PTPN14 in gastric canceru201d by Chen at al., copied at the end of this update, published June 26 2020. The Editorial Office has contacted the listed authors of the Bioscience Reports article to investigate the origins of the images in Figures 2A and B, sections of which had been published previously in Eur Rev Med Pharmacol Sci. 2017; 21 (8): 1759-1767. Whilst the images were published first in European Review for Medical and Pharmacological Sciences, the images in the Bioscience Reports article show a wider field of view. The authors of the Bioscience Reports article have corresponded with the Editorial Office and have provided the Editorial Office with raw data pertaining to these images, in which no further issues have been identified. The Editorial Office has also contacted the authors of the European Review for Medical and Pharmacological Sciences article as well as the publisher, Verduci Editore, for further information on whether raw data was available or received during the submission process, but received no response from the authors and no response from the publisher pertaining to the data from the Eur Rev Med Pharmacol Sci. article. The authors of the Bioscience Reports article, who have cooperated fully with the Bioscience Reports Editorial Office during this investigation, state that these image are their own original work, and given that the Editorial Office has not identified any further issues, the article will remain published with an Expression of Concern to alert readers to the duplicated images and prior publication. , 
 #3 Perhaps addressing the journal s concern about the originality of the images, I note that one additional image in Figure 2A of this paper also seems to have been previously published in Figure 2e of Cell Death and Disease (2015), doi: 10.1038 cddis.2014.573. I did not see any authors in common between these two papers. *** ![file] ,