Hsa_circ_0000673 is down-regulated in gastric cancer and inhibits the proliferation and invasion of tumor cells by targetting miR-532-5p
Authors: Peng Chang
Furong Wang
Yumin Li
Journal: Bioscience Reports (2018)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Hsa_circ_0000673; Invasion; cell proliferation; gastric cancer; miR-532-5p.
Affiliations: School of Life Sciences, Lanzhou University, Lanzhou 730000, China., Lanzhou University Second Hospital, Lanzhou 730000, China., Department of Pathology, Lanzhou University Second Hospital, Lanzhou 730000, China., The Key Laboratory of the Digestive System Tumors of Gansu Province, Lanzhou University Second Hospital, Lanzhou 730000, China., School of Life Sciences, Lanzhou University, Lanzhou 730000, China ., Department of General Surgery, Lanzhou University Second Hospital, Lanzhou 730000, China.

Abstract:  Circular RNAs (circRNAs), a new class of endogenous non-coding RNAs, have recently been known to play critical roles in various cellular biological processes, including tumorigenesis, in which they act as an miRNA sponge that regulates gene expression. Thus, revealing the functions of circRNAs in carcinogenesis and cancer development has been of great interest. However, their expression and functions in gastric cancer (GC) development are still largely unknown. Therefore, the present study aimed to identify novel deregulated circRNAs in GC and reveal their biological functions and molecular mechanisms in GC. Quantitative real-time PCR (qRT-PCR) was performed to measure the expression levels of circRNAs in GC tissues, cell lines, and plasma. The MTT assay, colony formation assay, transwell assay, and tumor xenografts in vivo were used to evaluate the effects of circRNAs on the proliferation and invasion of GC. The abovementioned methods coupled with Western blotting were used to investigate the molecular mechanisms. The current study showed that hsa_circ_0000673 was significantly down-regulated in GC. Overexpression of hsa_circ_0000673 inhibited the proliferation and invasion of GC cells. In contrast, hsa_circ_0000673 down-regulation promoted the proliferation and invasion of GC cells. Further studies revealed that hsa_circ_0000673 targetted miR-532-5p and up-regulated the expression of RUNX3. The present study showed that hsa_circ_0000673 was decreased in GC and it exerted tumor-suppressing effects by targetting miR-532-5p and up-regulating RUNX3 expression level. Hsa_circ_0000673 may be a promising diagnosis biomarker and therapeutic target in GC. 

Retracted: No

PubPeer comments:
 In Figure 3C, a micrograph has been duplicated and presented as something else. In Figure 6D, a micrograph has been duplicated and presented as something else. ![file] , 
 Dear Sir Madam, Thank you for notifying us of the potential errors in our published paper. Your points are taken seriously. We will investigate this issue and respond to you as soon as we possibly can. Yours sincerely, Yumin Li , 
 EXPRESSION OF CONCERN| FEBRUARY 16 2021 The Editorial Office has been made aware of potential issues surrounding the scientific validity of this paper, hence has issued an expression of concern to notify readers whilst the Editorial Office investigates. , 
 Additionally, it seems a fair amount of data is based on known problematic cell lines: BGC-823 (RRID:CVCL_3360) Problematic cell line: Contaminated. Shown to be a HeLa derivative (CCRID; PubMed=26116706; PubMed=28851942). Originally thought to be a gastric adenocarcinoma. SGC-7901 (RRID:CVCL_0520) Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=26116706; PubMed=28851942). Originally thought to originate from the lymph node of a 56 year old female patient with a gastric carcinoma. MGC-803 (RRID:CVCL_5334) Problematic cell line: Possibly contaminated. May be a derivative of HeLa and another cell of unknown origin. The STR profile is highly similar to that of HeLa yet shows some specific differences that suggest a possible hybrid cell line. MKN28 (RRID:CVCL_1416) Problematic cell line: Contaminated. Shown to be a MKN74 derivative (PubMed=20143388). source:[(http: web.expasy.org cellosaurus )] , 
 Figure 6 panels in this paper also appear to have been used in an other paper by the same group, i.e. Yucai Wei et al., Aging (2019), DOI: 10.18632 aging.102359 - https: pubpeer.com publications 82580BC68BF23D812375192E890CAD In that paper, the panels represent very different experiments. ![file] , 
 Retracted [14 July 2022] . --------------------- This article is being retracted from Bioscience Reports at the request of the Editor-in-Chief and the Editorial Board following receipt of a notification from several readers, alerting the Editorial Board to duplications in Figures 3C and 6D, after which a third duplication in Figure 6D was identified. Readers also identified that several panels from Figures 6C and D also appeared in the groupu2019s previous publication (Wei et al. LDLRAD2 overexpression predicts poor prognosis and promotes metastasis by activating Wnt u03b2-catenin EMT signaling cascade in gastric cancer. Aging (Albany NY). 2019 Oct 24;11(20):8951-8968. doi: 10.18632 aging.102359). The authors have contacted the Journal to correct the Article however, given they were unable to provide the original images and the extent of the issues raised, the Editorial Board stand by the decision to retract the article. The authors do not agree to the Retraction. ,