Astragaloside IV inhibits progression of glioma via blocking MAPK/ERK signaling pathway
Authors: Fei Wang
Wen Shen
Bin Li
Tao Huang
Ningtao Liu
Journal: Biochemical and biophysical research communications (2017)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: Astragaloside IV; Glioma; MAPK/ERK; Metastasis; Proliferation.
Affiliations: Department of Neurosurgery, Tongji Hospital, Shanghai, 200065, China., Department of Clinical Laboratory, The Baoshan Branch of Shanghai First People's Hospital, Shanghai, 200940, China., Department of Clinical Laboratory, The Baoshan Branch of Shanghai First People's Hospital, Shanghai, 200940, China. Electronic address: .

Abstract:  Glioma is one of the most common primary brain tumors in adults with a high mortality rate and relapse rate. Thus, finding better effective approaches to treat glioma has become very urgent. Astragaloside IV (AS-IV), the major active triterpenoid in Radix Astragali, has shown anti-tumorigenic properties in certain cancers. However, its role in glioma remains unclear. Here, we studied the effects of AS-IV on glioma in vitro and in vivo, and explored the underlying mechanisms. Our results revealed that AS-IV dose-dependently inhibited the proliferation of U251 cells in vitro and attenuated tumor growth in vivo. In addition, the migration and invasion ability of U251 cell has been suppressed in presence of AS-IV. The levels of proliferating cell nuclear antigen (PCNA), Ki67, matrix metallopeptidase (MMP) -2, MMP-9 and vascular endothelial growth factor (VEGF) were decreased significantly by the treatment of different concentrations AS-IV. Furthermore, AS-IV also significantly weakened the activation of Mitogen-activated protein kinase/Extracellular regulated protein kinase (MAPK/ERK) signaling pathway in vitro and in vivo. Taken together our study has identified a novel function of AS-IV and provided a molecular basis for AS-IV potential applications in the treatment of glioma and other cancers. 

Retracted: No

PubPeer comments:
 Please be noted, the same or very similar crease of Surgical pad are showing on the following 11 chinese papers including this paper. Also, the samples on the pad are somehow shared in those papers. UCA1 Regulates the Growth and Metastasis of Pancreatic Cancer bySponging miR-135a. Oncology research (2017) . DOI: 10.3727 096504017x14888987683152. ![file] H19 promotes the migration and invasion of colon cancer by spongingmiR-138 to upregulate the expression of HMGA1. INTERNATIONAL JOURNAL OF ONCOLOGY (2017). DOI: 10.3892 ijo.2017.3941. ![file] Silencing of lncRNA CCDC26 Restrains the Growth and Migration of Glioma Cells In Vitroand In Vivo via Targeting miR-203. Oncology research (2018). DOI: 10.3727 096504017x14965095236521. ![file] Long Noncoding RNA SChLAP1 Accelerates the Proliferation and Metastasis of Prostate Cancervia Targeting miR-198 and Promoting the MAPK1 Pathway. Oncology research (2018) . DOI: 10.3727 096504017x14944585873631. ![file] HOTAIR contributes to cell proliferation and metastasis of cervical cancer via targetting MAPK1 axis. Bioscience reports (2018). DOI: 10.1042 bsr20171563. ![file] Long non-coding RNA MEG3 inhibits cell growth of gliomas by targeting miR-93 andinactivating PI3K AKT pathway. Oncology reports (2017). DOI: 10.3892 or.2017.5871. ![file] The inhibitory effect of MEG3 miR-214 AIFM2 axis on the growth of T-cell lymphoblastic lymphoma. INTERNATIONAL JOURNAL OF ONCOLOGY. DOI: 10.3892 ijo.2017.4006. ![file] Inhibition of BAMBI reduces the viability and motility of colon cancervia activating TGFu2011u03b2 Smad pathway in vitro and in vivo. ONCOLOGY LETTERS. 10.3892 ol.2017.6811. ![file] HNF1Au2011AS1 promotes growth and metastasis of esophageal squamous cellcarcinoma by sponging miRu2011214 to upregulate the expression of SOX-4. INTERNATIONAL JOURNAL OF ONCOLOGY. DOI: 10.3892 ijo.2017.4034. ![file] Astragaloside IV inhibits progression of glioma via blocking MAPK ERKsignaling pathway. Biochemical and Biophysical Research Communications (2017). DOI: 10.1016 j.bbrc.2017.07.052. ![file] Epigenetic silence of HOTAIR contributes to the metastasis of pancreaticcancer via targeting miR-138. Oncology Research (2017). DOI: 10.3727 096504017X15138544374391. ![file] Possibly the following one: Long Noncoding RNA MEG3 Inhibits Cell Proliferation and Metastasis in Chronic Myeloid Leukemia via Targeting miR-184. Oncology research (2018) . DOI: 10.3727 096504017x14980882803151 ![file] , 
  Fig. 2. AS-IV suppresses migration and invasion of glioma cells. (A) Matrigel invasion assay . ![file] Green, red, purple rectangles are for comparison with Fig 4A of [Li et al (2018)] . ![file] Other manifestations in that thread. , 
 Fig 1C,  The expressions of Ki67, PCNA were measured by Western blot . ![file] Enhanced contrast reveals areas of repeating background within the PCNA band. The Ki67 band is strange in appearance, with one background texture just above the central WB-shaped phenomena and a different, vertically-stretched background texture along the top and bottom, separated by sharp horizontal discontinuities. I have not examined the other WBs. , 
 *[left]* Fig 1C again. *[right]* Fig 1C from  Silencing of H19 inhibits the adipogenesis and inflammation response in ox-LDL-treated Raw264.7 cells by up-regulating miR-130b ([Han et al 2018] ). ![file] *[left]* Fig 2C. *[right]* Fig 2C from Han et al (2018). ![file] These come under the jurisdiction of the Department of Unexpected Similarities. ,