LncRNA ITGB1 promotes the development of bladder cancer through regulating microRNA-10a expression
Authors: Y-J Niu
Z-Q Shang
C-M Chai
L Dai
T-Y Shen
Y Tian
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: lncRNA ITGB1; bladder cancer; cell proliferation; microRNA-10a; progression
Affiliations: Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin, China. .

Abstract:  Objective: This study aims to investigate the expression level of lncRNA ITGB1 both in bladder cancer (BCa) tissue and cell lines, as well as to evaluate its function and potential mechanism in the progression of BCa. Patients and methods: The expressions of lncRNA ITGB1 in 36 BCa tissues samples (and corresponding adjacent normal ones) and cell lines were detected by quantitative real-time polymerase chain reaction (qRT-PCR). After transfection of sh-ITGB1 in BCa cell lines, the effect of ITGB1 on the proliferation of BCa cells was examined by cell counting kit-8 (CCK-8) assay and colony formation assay. Subsequently, qRT-PCR was used to examine microRNA-10a expression in BCa tissues and cells after ITGB1 was silenced. At the same time, the correlation between ITGB1 and microRNA-10a expression was analyzed. Finally, cell recovery experiment was applied for the in-depth study of the interaction between ITGB1 and microRNA-10a and its underlying mechanism. Results: LncRNA ITGB1 was found upregulated in BCa tissues and cell lines. Knockdown of lncRNA ITGB1 remarkably inhibited cell proliferation. The expression levels of ITGB1 and microRNA-10a in BCa tissues were negatively correlated. ITGB1 downregulation was found to be able to enhance microRNA-10a expression, suggesting that microRNA-10a may be a potential target for ITGB1 in BCa. In addition, cell reverse experiment also verified that ITGB1 could regulate the expression of microRNA-10a, and their interaction affected the malignant progression of BCa. Conclusions: LncRNA ITGB1 level is upregulated in BCa tissues and associated with the pathological stage of BCa, which could be used as a new predictor of BCa patients' prognosis. In addition, ITGB1 might promote BCa cell proliferation via regulating microRNA-10a expression. 

Retracted: No

PubPeer comments:
 Figs 1C and 3F.  Kaplan Meier survival curve of bladder cancer patients based on ITGB1 expression ...  Kaplan-Meier survival curves of bladder cancer patients based on miR-10a expression . It is not possible for a single set of 44 survival times to be dichotomised two ways and produce these two different K-M plots. ![file] They are also two more manifestations of  Hoya s Law for J.BUOn ERMPS :  When 40 or 50 cases of any given cancer are dichotomised into half-groups of high- or low-expression of any given noncoding RNA, survival rate for one half-group or both *always* drops to 0% at 75 months. Some other examples, from left to right: - Fig 1 from [Zang et al (2020)] - Fig 1 from [Li et al (2020)] - Fig 1, and Fig 4D, from [Cui et al (2019)] - Fig 1D from [Gao et al (2019)] . ![file] *[left]* Figure 1C from [Wu et al (2020)] . *[right]* Figs 1D, 3E from [Wu et al (2019)] . ![file] 1D from [Luo, Yan Liu] : ![] , 
 Fig 3E.  There was a significant negative correlation between the expression levels of ITGB1 and miR-10a in bladder cancer tissues . At right, an overlay of 20 correlation scatterplots from various ERMPS papers, all based on the same spreadsheet, emphasising the points frequently occurring between them. ![file] ,