Migration of growth factor-stimulated epithelial and endothelial cells depends on EGFR transactivation by ADAM17
Authors: Thorsten Maretzky
Wenhui Zhou
Shahin Rafii
Pui-Mun Wong
Carl P Blobel
Steven L Swendeman
Astrid Evers
Karina Reiss
Journal: Nature Communications (2011)
Discipline: Multidisciplinary
Keywords: FGFR2b; VEGFR2; cell migration; proliferation; embryonic development
Affiliations: Arthritis and Tissue Degeneration Program, Hospital for Special Surgery at Weill Medical College, Cornell University, New York, New York 10021, USA.

Abstract:  The fibroblast growth factor receptor 2-IIIb (FGFR2b) and the vascular endothelial growth factor receptor 2 (VEGFR2) are tyrosine kinases that can promote cell migration and proliferation and have important roles in embryonic development and cancer. Here we show that FGF7/FGFR2b-dependent activation of epidermal growth factor receptor (EGFR)/ERK1/2 signalling and cell migration in epithelial cells require stimulation of the membrane-anchored metalloproteinase ADAM17 and release of heparin-binding epidermal growth factor (HB-EGF). Moreover, VEGF-A/VEGFR2-induced migration of human umbilical vein endothelial cells also depends on EGFR/ERK1/2 signalling and shedding of the ADAM17 substrate HB-EGF. The pathway used by the FGF7/FGFR2b signalling axis to stimulate shedding of substrates of ADAM17, including ligands of the EGFR, involves Src, p38 mitogen-activated protein-kinase and PI3K, but does not require the cytoplasmic domain of ADAM17. Based on these findings, ADAM17 emerges as a central component in a triple membrane-spanning pathway between FGFR2b or VEGFR2 and EGFR/ERK1/2 that is required for cell migration in keratinocytes and presumably also in endothelial cells. 

Retracted: No

PubPeer comments:
 This paper has features suggestive of [what has elsewhere been labeled as  Papermill #2 ] , characterised by stylised geometrical WBs that are reduced to black blobs on a featureless grey background, reminiscent of a Miro painting: ![file] ...and by a small repertoire of recurring flow cytometry plots, ![file] slightly modified by artisanal stippling on each of their occurrences. ![] Other examples are listed in a Google document. https: docs.google.com spreadsheets d 1qBqQMXgzxoBd5bTbqdMuapGheupE5bdIopypju417NM edit?usp=sharing The connection between the name of the nominal corresponding author and the corresponding email address is not obvious. ![file] , 
 Retracted. No date or Retraction Note, but Abstract now reads  *Cancer Biotherapy and Radiopharmaceuticals* officially retracts the paper entitled, u201cmiR-205 Promotes Apoptosis of Cervical Cancer Cells and Enhances Drug Sensitivity of Cisplatin by Inhibiting YAP1,u201d by Xingmei Li, Yuewen Li, Yuning Han, Bing Dong, Dan Liu, Liqun Che, Yu Liu, and Yuchun Wang (Cancer Biother Radiopharm. 2020;35(5):338u2013344; doi: 10.1089 cbr.2019.2983) due to the discovery that the paper was submitted from a paper mill. This is a violation of the journal s standard protocols and is considered an infraction against the rigorous standards of scientific publishing.  The Editor and Publisher of *Cancer Biotherapy and Radiopharmaceuticals* are committed to preserving the scientific literature and the community it serves, and does not tolerate any misconduct. ,