<p>LncRNA LINC01116 Contributes to Cisplatin Resistance in Lung Adenocarcinoma</p>
Authors: Chenchen Wei
Weiyan Zou
Zhaoxia Wang
Fen Yang
Jin Gao
Qinnan Chen
Junbin Wang
Journal: OncoTargets and Therapy (2020)
Discipline: CLINICAL MEDICINE
Keywords: LINC01116; chemotherapy resistance; cisplatin; epithelial–mesenchymal transition; lung adenocarcinoma.
Affiliations: Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, People's Republic of China., Department of Histology and Embryology, Bengbu Medical College, Bengbu 233030, People's Republic of China., Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing 211166, People's Republic of China.

Abstract:  Background: Long non-coding RNAs (lncRNAs) have been found to contribute to cisplatin resistance in several cancers; however, the role of lncRNA LINC01116 in cisplatin resistance remains unknown in non-small-cell lung cancer. This study aimed to examine the contribution of LINC01116 to cisplatin resistance in lung adenocarcinoma (LAD). Materials and methods: Cisplatin-resistant A549/DDP cells were generated by treatment with cisplatin by dose escalation. LINC01116 expression was compared between A549 and A549/DDP cells, and between cisplatin-resistant and non-resistant LAD specimens. The cell viability, colony formation, proliferation, migration and invasion were measured using MTT and Transwell assays, and cell apoptosis and cell cycle were detected using flow cytometry. The expression of E-cadherin and Vimentin was quantified. LAD xenografts were modeled in nude mice to investigate the role of LINC01116 on the resistance of LAD to cisplatin. Results: MTT assay measured the IC50 values of 13.49 ± 1.62 and 3.52 ± 1.33 μg/mL for A549/DDP and A549 cells, respectively. LINC01116 was overexpressed in cisplatin-resistant LAD specimens and A549/DDP cells (P &lt; 0.05). Knockdown of LINC01116 inhibited cell viability, proliferation, migration and invasion, promoted apoptosis and enhanced the sensitivity to cisplatin in A549/DDP cells, while LINC01116 overexpression promoted cell viability, proliferation, migration and invasion, inhibited apoptosis and reduced the sensitivity to cisplatin in A549 cells. LINC01116 knockdown resulted in a 2.1-fold increase in E-cadherin expression and a 56% reduction in Vimentin expression in A549/DDP cells, and LINC01116 overexpression resulted in a 45% reduction in E-cadherin expression and a 1.82-fold increase in Vimentin expression in A549 cells. Conclusion: Dysregulation of lncRNA LINC01116 expression results in resistance of LAD to cisplatin via the EMT process. Our findings support the oncogenic role of LINC01116 to promote the development of cisplatin resistance in LAD, and LINC01116 may be a novel predictor of poor response to cisplatin. 

Retracted: No

PubPeer comments:
 Two images in Figure 2E appear to overlap, with different aspect ratio. *** ![file] , 
 #1 There appears to be similar issues with Figure 5E, again with alterations to the aspect ratio. Difficult to see how the scale of the images could be accidentally changed during figure assembly. *** ![file] , 
 Is it common for rulers at labs in China to use the Imperial scale? Aren t SI Metric rulers preferred by journals? *** ![file] , 
 Thank you very much for your concern reminder. We contacted the researchers in these days to provide the original data graphs, and found that the pictures was misused because the pictures was stored at the same folder. This is a stupid error. We will contact the publisher to erupt the figures.We offer pictures of the rule and background plates filmed yesterday, which proves that all experiments are true. Thank you again for your reminder.![file] ![file] , 
 #4 Respectfully, the images do not appear to only have been mistakenly selected. They have also been stretched horizontally and vertically differently from each other (changing the aspect ratio). What explanation would account for the changing of scale relative to the other images? Regarding the ruler, my question was not asking the authors to demonstrate that they had access to an Imperial scale ruler. My question was: why was an Imperial scale ruler used at all? This seems unusual. ,