Long non‐coding RNA LINC00152 promotes cell growth and invasion of papillary thyroid carcinoma by regulating the miR‐497/BDNF axis
Authors: Peisong Wang
Shuai Xue
Guang Chen
Mingcui Zang
Xun Guo
Zhihui Sun
Journal: Journal of Cellular Physiology (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: LINC00152; brain-derived neurotrophic factor (BDNF); miR-497; papillary thyroid carcinoma (PTC).
Affiliations: Department of Drug Management, The First Hospital of Jilin University, Changchun, Jilin, China., Department of Hyperbaric Oxygen Therapy, The First Hospital of Jilin University, Changchun, Jilin, China., Department of Hepatobiliary pancreatic surgery, The First Hospital of Jilin University, Changchun, Jilin, China., Department of Thyroid surgery, The First Hospital of Jilin University, Changchun, Jilin, China.

Abstract:  Long intergenic non-coding RNA 152 (LINC00152) was reported to be tightly linked to tumorigenesis and progression in multiple cancers. However, its biological role and modulatory mechanism in papillary thyroid carcinoma (PTC) has not been elucidated. In this study, we determined the expression levels of LINC00152 in PTC tissues and cell lines by quantitative real time polymerase chain reaction (qRT-PCR). Cell proliferation, colony formation, migration, and invasion were measured by a Cell Counting Kit-8 assay, colony formation analysis, wound healing, and transwell invasion assay, respectively. A luciferase reporter assay and qRT-PCR were used to determine whether LINC00152 interacts with miR-497 directly. We established a xenograft mouse model to examine the underlying molecular mechanism and effect of LINC00152 on tumor growth in vivo. We found that LINC00152 expression was significantly increased in PTC tissues and derived cell lines. LINC00152 knockdown significantly inhibited proliferation, colony formation, migration, and invasion in vitro, and impaired tumor growth in vivo. We revealed that LINC00152 functioned as a competing endogenous RNA to the miR-497 sponge, downregulating its downstream target brain-derived neurotrophic factor (BDNF), which is an oncogene in thyroid cancer. These findings suggest that LINC00152 is responsible for PTC cell proliferation and invasion and exerts its function by regulating the miR-497/BDNF axis. 

Retracted: No

PubPeer comments:
 Fig 6(d).  Immunohistochemical (IHC) staining of Kiu201067 in xenograft tumor. ![file] What happened here? , 
 Fig 6(b).  Tumor image . ![file] My faith in the veridicality of this line-up of tumors is undermined by too much familiarity with the ruler component of the composition. ![] Source:*[left]* Fig 6B from [Li et al (2018)] .  Tumor tissues were imaged after the mice were sacrificed . *[right]* Fig 3B from [Fei et al (2018)] .  Shown are images of xenograft tumors at 28 days post injection. ![] , 
 Retracted [20 October 2022] . --------------------- The above article, published online on 26 August 2018 in Wiley Online Library , has been retracted by agreement between the authors, the journal s Editor in Chief, Prof. Dr. Gregg Fields, and Wiley Periodicals LLC. The retraction has been agreed after the authors stated that the experimental data in the article could not be verified. The investigation additionally revealed inconsistencies in several image elements. Thus, the editors consider the conclusions of this article to be invalid. The authors were not available for a final confirmation of the retraction. ,