The roles of MASPIN expression and subcellular localization in non-small cell lung cancer
Authors: Wei Lei
Ning Gu
Cheng Chen
Xiao-Fei Wang
Da-Xiong Zeng
Jian-An Huang
Bo Liang
Yan-Bin Chen
Ye-Han Zhu
Journal: Bioscience reports (2020)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: MASPIN; SERPINB5; non-small cell lung cancer; prognosis.
Affiliations: Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China, Nanjing University of Chinese Medicine, Nanjing, China, The First Affiliated Hospital of Soochow University, Suzhu, China, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, China, The First Affiliated Hospital of Soochow University, Suzhou, China

Abstract:  Accumulating studies have confirmed that mammary serine protease inhibitor (MASPIN) plays an essential role in non-small cell lung cancer (NSCLC). However, results are still controversial or inconsistent. In the present study, we attempted to identify the clinical significance of MASPIN and its potential molecular roles in NSCLC. The correlation of MASPIN with prognosis and clinicopathological characteristics was assessed by meta-analysis. Additionally, the potential molecular mechanisms of MASPIN in NSCLC was also investigated through several online databases. A total of 2220 NSCLC patients from 12 high quality studies were included and the results indicated that up-regulated MASPIN nucleus and cytoplasm expression was associated with poor overall survival (OS) (hazard ratio (HR) = 1.43, 95% confidence interval (CI) = 1.01-2.04, P&lt;0.05), elevated MASPIN cytoplasm expression was associated with poor OS (HR = 1.45, 95% CI = 1.01-2.07, P&lt;0.05), disease-free survival (DFS) (HR = 1.95, 95% CI = 1.31-2.88, P=0.001), and disease-specific survival (DSS) (HR = 2.17, 95% CI = 1.18-3.99, P=0.013). MASPIN both nucleus and cytoplasm location were associated with clinicopathological characteristics. Bioinformatics analysis validated the above results and suggested that human serpin family B member 5 (SERPINB5) hypomethylated levels were negatively correlated with its mRNA expression. Bioinformatics analysis also revealed the 85 most frequently altered neighboring genes of SERPINB5, and gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed 20 GO terms and 3 KEGG pathways with statistical significance. MASPIN had a statistically negative correlation with NSCLC prognosis, functioning as an oncoprotein by hypomethylation and influencing specific pathways involving the 85 genes identified herein. MASPIN might be a promising prognostic signature in NSCLC. 

Retracted: No

PubPeer comments:
 Figure 6 appears to have a repeated image. *** ![file] , 
 Thank you very much for your interest in our work. When we revisited our work, we found Figure 6B appeared to include some repeated images, which probably due to our mistake during figure assembly. The corrected Figure 6 are attached below. We sincerely apologize for this unintentional error made before manuscript submission, and we failed to catch at the submission and subsequent review and publication steps. We have contacted the editor to modify the figure. Thanks again.![file] , 
 #2 Thank you for responding. , 
 Correction published: https: portlandpress.com bioscirep article 40 7 BSR-20200743_COR 225633 Correction-The-roles-of-MASPIN-expression-and The authors of the original article above article u201cThe roles of MASPIN expression and subcellular localization in non-small cell lung canceru201d (Bioscience Reports (2020) 40(5), DOI: 10.1042 BSR20200743) would like to correct Figure 6B, as the IHC images of LUAD and LUSC were repeated due to mis-operation in visualisation. The authors had mistakenly reused the image of LUSC (Antibody CAB009570) for LUAD (Antibody HPA019025). The authors declare that these corrections do not change the results or conclusions of this paper, and express their sincere apologies for any inconvenience that this error has caused to the readers. The corrected version of Figure 6 is presented here. ![file] ,