miR-96-5p enhances cell proliferation and invasion via targeted regulation of ZDHHC5 in gastric cancer
Authors: Xianrong Liu
Baolong Wang
Xiangtao Meng
Journal: Bioscience reports (2020)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: cell apoptosis; gastric adenocarcinoma; microRNA-96-5p; prognosis.
Affiliations: General Surgery, ShanXian Hygeia Hospital, Heze, Shandong Province, China., Outpatient Department, ShanXian Central Hospital, Heze, Shandong Province, China., General Surgery, DongDa Hospital of ShanXian, Heze, Shandong Province, China.

Abstract:  Objective: To explore the biological function and mechanism of miR-96-5p in gastric cancer. Methods: The expression of differently expressed microRNAs (DEMs) related to gastric adenocarcinoma (GAC) prognosis was identified in GAC tumor samples and adjacent normal samples by qRT-PCR. A target gene miR-96-5p was selected using TargetScan, miRTarBase, miRDB databases. The combination of miR-96-5p and ZDHHC5 was verified by luciferase receptor assay. To further study the function and mechanism of miR-96-5p, we treated MGC-803 cells with miR-96-5p inhibitor and si-ZDHHC5, then detected cell viability, apoptosis, migration and invasion ability, as well as the expression of ZDHHC5, Bcl-2, Bax, cleaved caspase-3, cleaved caspase-9, and COX-2 by Western blot. Results: Compared with adjacent normal samples, the levels of miR-96-5p, miR-222-5p, and miR-652-5p were remarkably increased, while miR-125-5p, miR-145-3p, and miR-379-3p were significantly reduced in GAC tumor samples (P&lt;0.01), which were consistent with bioinformatics analysis. Furthermore, ZDHHC5 was defined as a direct target gene of miR-96-5p. miR-96-5p silence significantly reduced cell viability, increased cell apoptosis, and suppressed cell migration and invasion, as well as inhibited the expression of Bcl-2 and COX-2 and promoted Bax, cleaved caspase-3 and cleaved caspase-9 level in MGC-803 cells (P&lt;0.01). Notably, ZDHHC5 silence reversed the inhibiting effects of miR-96-5p on MGC-803 cells growth and metastasis Conclusion: Our findings identified six microRNAs (miRNAs; miR-96-5p, miR-222-5p, miR-652-5p, miR-125-5p, miR-145-3p, and miR-379-3p) related to GAC prognosis, and suggested that down-regulated miR-96-5p might inhibit tumor cell growth and metastasis via increasing ZDHHC5 expression enhance MGC-803 cell apoptosis, as well as decrease MGC-803 cell metastasis. 

Retracted: Yes

PubPeer comments:
 This article (Bioscience Reports (2020) 40(4), DOI: 10.1042 BSR20191845) is being retracted from Bioscience Reports at the request of the Editor-in-Chief and the Editorial Board following receipt of a notification from a reader, alerting the Editorial Board that the article had already been published in World Journal of Gastroenterology (DOI: 10.3748 wjg.v25.i47.6823), with a different authorship to the current paper. The authors listed on the Bioscience Reports paper have been contacted in regard to the retraction, but have failed to respond. , 
 2020 retraction. https: portlandpress.com bioscirep article 40 6 BSR-20191845_RET 225495 Retraction-miR-96-5p-enhances-cell-proliferation This article (Bioscience Reports (2020) 40(4), DOI: 10.1042 BSR20191845) is being retracted from Bioscience Reports at the request of the Editor-in-Chief and the Editorial Board following receipt of a notification from a reader, alerting the Editorial Board that the article had already been published in World Journal of Gastroenterology (DOI: 10.3748 wjg.v25.i47.6823), with a different authorship to the current paper. The authors listed on the Bioscience Reports paper have been contacted in regard to the retraction, but have failed to respond. , 
 #2 The two versions seem to have been submitted and processed concurrently. This version:  Received: July 10 2019 Revision Received: December 13 2019 Accepted: January 20 2020 Accepted Manuscript online: March 23 2020 The WJG version:  Peer-review started: September 23, 2019 First decision: November 4, 2019 Article in press: November 29, 2019 . It is natural to wonder how many other manuscripts submitted to Bioscience Reports have also been concurrently submitted to WJG, with different authorship and slight changes in the nature of the cancer. ,