<p>LncRNA MALAT1 Promotes the Proliferation, Migration, and Invasion of Melanoma Cells by Downregulating miR-23a</p>
Authors: Pan Wang
Liu Hu
Guili Fu
Jingjing Lu
Ying Li
Yuanquan Zheng
Lin Jia
Journal: Cancer Management and Research (2020)
Discipline: CLINICAL MEDICINE
Keywords: long-chain non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (lncRNA MALAT1); malignant proliferation; melanoma; miR-23a.
Affiliations: Department of Dermatology, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430015, Hubei Province, People's Republic of China., Department of Radiotherapy, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, 430071, People's Republic of China., Department of Nephrology, The Central Hosptial of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430015, Hubei Province, People's Republic of China.

Abstract:  Purpose: This study was designed to investigate the relationship between long-chain non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (lncRNA MALAT1)/miR-23a-23a and melanoma. Patients and methods: Fifty-two cases of corresponding non-tumor normal tissues and 109 cases (including 62 cases of primary melanoma and 47 cases of metastatic melanoma) were collected. Real-time fluorescent PCR quantified lncRNA MALAT1 and miR-23a, and counted the 3-year survival of high/low miR-23 and high/low lncRNA MALAT1 populations. We predicted the binding site according to the sequence information of lncRNA MALAT1 and miR-23a. lncRNA MALAT1 siRNA and miR-23a mimics vectors were constructed and transfected into melanoma cell lines respectively to observe their effects on cells. Results: Compared with corresponding non-tumor normal tissues, lncRNA MALAT1 in melanoma tissue increased while miR-23a decreased. Compared with primary melanoma, metastatic melanoma was higher and miR-23a was lower. Downregulation of lncRNA MALAT1 caused upregulation of miR-23a, and lncRNA MALAT1 could bind to miR-23a. Downregulating lncRNA MALAT1 or upregulating miR-23a inhibited cell proliferation, migration and invasion and promoted apoptosis. Rescue experiments revealed that downregulation of miR-23a could offset cell changes caused by downregulation of lncRNA MALAT1. Conclusion: lncRNA MALAT1 promotes malignant proliferation of melanoma cells through miR-23a. 

Retracted: No

PubPeer comments:
 Figure 2C. Much more similar than expected. https: imgur.com YT6vRBR , 
 No, these are not the same. There is a small bubble underneath the second band of the second highlighted section, which is absent from the first rectangle (have a close look). It is only because of these defining marks (scratches, blemishes, bubbles, smears, smudges etc) that two regions can possibly be compared. Without some defining feature, it s impossible to make a claim of similarity between blots like this, because they are just uniform fuzzy grey blobs from a paper that is so old, the authors surely wouldn t be expected to provide evidence to counter the claim. Ironically enough, in this specific example, it is the very presence of one of these defining features that clearly shows they are not the same panels.....something that must have been missed by Glabratella Mediterranensis. ,