Rosiglitazone Suppresses the Growth and Invasiveness of SGC-7901 Gastric Cancer Cells and Angiogenesis In Vitro via PPARgamma Dependent and Independent Mechanisms
Authors: Pinjin Hu
Huixin Chen
Qing He
Ruiping Pang
Xin Song
Minhu Chen
Jie Chen
Baili Chen
Journal: PPAR research (2008)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: thiazolidinediones; PPARgamma; anticancer effect; metastatic potential; angiogenesis
Affiliations: Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China., Department of Physiology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China, Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China

Abstract:  Although thiazolidinediones (TZDs) were found to be ligands for peroxisome proliferators-activated receptorgamma (PPARgamma), the mechanism by which TZDs exert their anticancer effect remains unclear. Furthermore, the effect of TZDs on metastatic and angiogenesis potential of cancer cells is unknown. Our results in this paper show that rosiglitazone inhibited SGC-7901 gastric cancer cells growth, caused G1 cell cycle arrest and induced apoptosis in a dose-dependent manner. The effects of rosiglitazone on SGC-7901 cancer cells were completely reversed by treatment with PPARgamma antagonist GW9662. Rosiglitazone inhibited SGC-7901 cell migration, invasiveness, and the expression of MMP-2 in dose-dependent manner via PPARgamma-independent manner. Rosiglitazone reduced the VEGF induced angiogenesis of HUVEC in dose-dependent manner through PPARgamma-dependent pathway. Moreover, rosiglitazone did not affect the expression of VEGF by SGC-7901 cells. Our results demonstrated that by PPARgamma ligand, rosiglitazone inhibited growth and invasiveness of SGC-7901 gastric cancer cells and angiogenesis in vitro via PPARgamma-dependent or -independent pathway. 

Retracted: Yes

PubPeer comments:
 Heavy reuse of data in Figure 3. ![file] , 
 2020 retraction. https: www.hindawi.com journals ppar 2020 9469261 Retraction | Open Access Volume 2020 |Article ID 9469261 | https: doi.org 10.1155 2020 9469261 Show citation Retracted: Rosiglitazone Suppresses the Growth and Invasiveness of SGC-7901 Gastric Cancer Cells and Angiogenesis In Vitro via PPARu03b3 Dependent and Independent Mechanisms PPAR Research Received 16 Aug 2020 Accepted 16 Aug 2020 Published 01 Sep 2020 PPAR Research has retracted the article titled u201cRosiglitazone Suppresses the Growth and Invasiveness of SGC-7901 Gastric Cancer Cells and Angiogenesis In Vitro via PPARu03b3 Dependent and Independent Mechanismsu201d due to figure duplication. As raised on PubPeer [2], Figure 3C shows several issues: (i)tFor PPARu03b3, lanes 1, 2, and 3 appear similar (ii)tFor PPARu03b3, lanes 6, 7, 9, and 11 all appear similar (iii)tFor PPARu03b3, lanes 8 and 10 appear similar (iv)tFor MMP-2, lanes 1 and 7 appear similar; likewise lanes 3 and 8 (v)tFor VEGF, lanes 8 and 11 appear similar (vi)tFor u03b2-actin, lanes 1, 2 appear similar to lanes 7, 8 (vii)tFor u03b2-actin, lanes 5, 9, and 11 appear similar At revision, the authors replaced the Western Blots in Figures 2B and 2D in the submitted article with new Western Blots in Figure 3C to consolidate the results into one blot and add results for VEGF with GW9662, which had been missing in the submitted version. The authors say Figure 3C does match the text description in the article, is not their experimental result, and they do not know how it was included in the article. References Q. He, R. Pang, X. Song et al., u201cRosiglitazone Suppresses the Growth and Invasiveness of SGC-7901 Gastric Cancer Cells and Angiogenesis In Vitro via PPARu03b3 Dependent and Independent Mechanisms,u201d PPAR Research, vol. 2008, Article ID 649808, 9 pages, 2008. Q. He, R. Pang, X. Song et al., u201cRosiglitazone Suppresses the Growth and Invasiveness of SGC-7901 Gastric Cancer Cells and Angiogenesis In Vitro via PPARgamma Dependent and Independent Mechanisms,u201d PPAR Research, vol. 2008, Article ID 649808, 9 pages, 2008. ,