Aberrant expression miR-196a is associated with abnormal apoptosis, invasion, and proliferation of pancreatic cancer cells
Authors: Hui Chen
Yiqi Du
Hongyu Wu
Jun Gao
Xiangyu Kong
Yanfang Gong
Jianqiang Liu
Zhaoshen Li
Minghao Liu
Journal: Pancreas (2013)
Discipline: CLINICAL MEDICINE
Keywords: miR-196a; pancreatic adenocarcinoma; ING5; apoptosis; invasion
Affiliations: From the Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai, China.

Abstract:  Objectives: MiR-196a levels inversely correlated with survival in pancreatic adenocarcinoma patients. However, the functional contributions of miR-196a to pancreatic cancer remain unclear. Methods: Three lentiviral vectors encoding microRNA miR-196a precursor, inhibitor, and scrambled microRNA oligomer were transfected into Panc-1 cells, respectively. Then we explored the regulation of inhibitor of growth 5 (ING5) expression by miR-196a and its impact on apoptosis, invasion, and growth of pancreatic cancer cells. The lentiviral transfected Panc-1 cells were surgically implanted into the pancreas of mice. In vivo tumor growth and ING5 expression were measured. Results: Down-regulation of ING5 expression was detected in cells transfected with miR-196a precursor (P &lt; 0.01), accompanied by less apoptosis, increased invasion, and proliferation compared with control cells (P &lt; 0.05). Cells transfected with miR-196a inhibitor revealed an opposite trend. Smaller detectable tumors were found in only 60% of mice after implantation of Lenti.miR-196a inhibitor-transfected Panc-1 cells compared with controls (360.7 ± 303.6 mm vs 511.58 ± 365.9 mm in controls; P &lt; 0.01). Conclusion: Our results provide experimental evidence to support aberrant expression of miR-196a is associated with abnormal apoptosis, invasion, and proliferation of pancreatic cancer cells. 

Retracted: No

PubPeer comments:
 FIGURE 7. Effect of miR-196a on invasion of Panc-1 cells. Panc-1 cells Panc-1 cells were treated with scrambled RNA oligomer (B), and miR-196a anti-miR miRNA inhibitors (D) for 48 hours B and D  **simply circles cookie-cut out from a larger image**! The left image was used an online software to spot the manipulation at the first place because it is really hard to find it at first glance. ![file] Using the same good skill  cookie-cut as the following: https: pubpeer.com publications 604D62C41AC59501A483536332C691#9 Betulinic acid impairs metastasis and reduces immunosuppressive cells in breast cancer models Oncotarget (2018) - 9 Comments doi: 10.18632 oncotarget.23376 issn: 1949-2553 pubmed: 29423083 An-Qi Zeng , Yan Yu , Yu-Qin Yao , Fang-Fang Yang , Mengya Liao , Lin-Jiang Song , Ya-Li Li , Yang Yu , Yu-Jue Li , Yuan-Le Deng , Shu-Ping Yang , Chen-Juan Zeng , Ping Liu , Yong-Mei Xie , Jin-Liang Yang , Yi-Wen Zhang author has email , Ting-Hong Ye author has email , Yu-Quan Wei ![file] **** , 
 Dear Friends, Thank you for your letter and pointing out the mistakes in our article. Although we have all the original data and they fully support the conclusions of the paper, we regret that we accidentally made mistakes in process cut the figure(Figure 6B and D). We have revisited all the raw figure of these experiments. The corrected figure 6 and partial raw figure was attached below. The raw figure proves that we have completed the experiment. Take a few pictures with a microscope camera for aesthetics. The raw figure taken by mobile phone during the experiment, the figure used in this paper were taken by microscope camera, so it is no longer possible to match the figure taken by mobile phone with those taken by microscope. We sincerely apologize for this unintentional error made before manuscript submission but we failed to catch at the submission and subsequent review and publication steps. We stand by the quality of the experiments and the conclusions made in the paper. We have contacted the editorial office of Pancreas and already approved for the correction, and the corrected data will be published very soon. , 
 Dear Friends, Thank you for your letter and pointing out the mistakes in our article. Although we have all the original data and they fully support the conclusions of the paper, we regret that we accidentally made mistakes in process cut the figure(Figure 7B and D). We have revisited all the raw figure of these experiments. The corrected figure 7 and partial raw figure was attached below. The raw figure proves that we have completed the experiment. Take a few pictures with a microscope camera for aesthetics. The raw figure taken by mobile phone during the experiment, the figure used in this paper were taken by microscope camera, so it is no longer possible to match the figure taken by mobile phone with those taken by microscope. We sincerely apologize for this unintentional error made before manuscript submission but we failed to catch at the submission and subsequent review and publication steps. We stand by the quality of the experiments and the conclusions made in the paper. We have contacted the editorial office of Pancreas and already approved for the correction, and the corrected data will be published very soon. ![file] ![file] , 
 Erratum, March 2020: https: journals.lww.com pancreasjournal Fulltext 2020 03000 Aberrant_Expression_miR_196a_is_Associated_With.26.aspx  In the article u201cAberrant Expression miR-196a is Associated With Abnormal Apoptosis, Invasion, and Proliferation of Pancreatic Cancer Cells,u201d published in the October 2013 issue of Pancreas, an error appears in Figure 7. The authors report that Figures 7B and D are the same original images due to a mistake in the selection of the images during the article writing process. The authors explain that because the experimental results pictures were very similar, the same picture was mistakenly used twice in file processing, resulting in duplication of the Figures 7B and D. The corrected figure appears below. ,