Suppression of microRNA-135b-5p protects against myocardial ischemia/reperfusion injury by activating JAK2/STAT3 signaling pathway in mice during sevoflurane anesthesia
Authors: Ya-Wei Chen
Dong-Mei Fan
Xiao-Juan Xie
Kai Xi
Liang Fang
Qian-Hui Li
Li-Gang Ma
Peng-Wei Qi
Journal: Bioscience Reports (2017)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Inhalation anesthesia; JAK2/STAT3 signaling pathway; MicroRNA-135b-5p; Myocardial ischemia/reperfusion injury; Sevoflurane.
Affiliations: Department of Anesthesiology, The First Affiliated Hospital of Henan University of Science and Technology (New District Hospital), Luoyang 471300, P.R. China ., Department of Obstetrics and Gynecology, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471300, P.R. China., Department of ENT, The First Affiliated Hospital of Henan University of Science and Technology (New District Hospital), Luoyang 471300, P.R. China., Department of Obstetrics and Gynecology, Luoyang Women and Children's Medical Health Care Center, Luoyang 471300, P.R. China., Department of Anesthesiology, The First Affiliated Hospital of Henan University of Science and Technology (New District Hospital), Luoyang 471300, P.R. China., Department of Anesthesiology, The Second Affiliated Hospital of Henan University of Science and Technology, Luoyang 471300, P.R. China.

Abstract:  The study aims to explore the effects of miR-135b-5p on myocardial ischemia/reperfusion (I/R) injuries by regulating Janus protein tyrosine kinase 2 (JAK2)/signal transducer and activator of transcription (STAT) signaling pathway by mediating inhalation anesthesia with sevoflurane. A sum of 120 healthy Wistar male mice was assigned into six groups. Left ventricular ejection fraction (LVEF) and left ventricular shortening fraction (LVSF) were detected. Cardiomyocyte apoptosis was determined by terminal dexynucleotidyl transferase mediated dUTP-biotin nick end labeling (TUNEL) assay. MiR-135b-5p expression, mRNA and protein expression of p-STAT3, p-JAK2, STAT3, JAK2, B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein B (Bax) were detected by quantitative real-time PCR (qRT-PCR) and Western blotting. Target relationship between miR-135b-5p and JAK2 was confirmed by dual-luciferase reporter assay. The other five groups exhibited increased cardiomyocyte necrosis, apoptosis, miR-135b-5p and Bax expression, mRNA expression of JAK2 and STAT3, and protein expression of p-STAT3 and p-JAK2 compared with the sham group, but showed decreased LVEF, LVFS, and Bcl-2 expression. Compared with the model and AG490 + Sevo groups, the Sevo, inhibitor + Sevo and inhibitor + AG490 + Sevo groups displayed reduced cardiomyocyte necrosis, apoptosis, miR-135b-5p and Bax expression, but displayed elevated mRNA expression of JAK2 and STAT3, protein expression of p-STAT3 and p-JAK2, LVEF, LVFS and Bcl-2 expression. Compared with the Sevo and inhibitor + AG490 + Sevo groups, the AG490 + Sevo group showed decreased LVEF, LVFS, Bcl-2 expression, mRNA expressions of JAK2 and STAT3, and protein expressions of p-STAT3 and p-JAK2, but increased cardiomyocyte necrosis, apoptosis, and Bax expressions. MiR-135b-5p negatively targetted JAK2. Inhibition of miR-135b-5p can protect against myocardial I/R injury by activating JAK2/STAT3 signaling pathway through mediation of inhalation anesthesia with sevoflurane. 

Retracted: No

PubPeer comments:
 Fig 1.  ECG of the mice in the sham and model group . ![file] Inquiring minds would like to know more about the hardware or software used to plot these palsied lines. , 
 Figures 4C, 5B. There are a number of familiar protein blobs in these WBs. ![file] , 
 Expression of Concern, [26 March 2021](10.1042 BSR-20170186_EOC).  The Editorial Office has been made aware of potential issues surrounding the scientific validity of this paper, hence has issued an expression of concern to notify readers whilst the Editorial Office investigates. , 
 Retracted [8 June 2021] . ---------------------------------------- This Retraction follows an Expression of Concern relating to this article previously published by Portland Press. This article is being retracted from Bioscience Reports at the request of the Editor-in-Chief and the Editorial Board following receipt of a notification from a reader, alerting the Editorial Board to the Western blots in Figures 4C and 5B which are duplicated in several other publications. The authors have been contacted in regards to the retraction, and have not responded to the Journal s queries and the concerns raised. Given the extent of the issues raised, the Editorial Board stand by the decision to retract the article. ,