Albumin Nano-Encapsulation of Piceatannol Enhances Its Anticancer Potential in Colon Cancer Via Downregulation of Nuclear p65 and HIF-1α
Authors: Paul Thompson
Saurabh Satija
Prashant Kesharwani
Ibrahim M Azzouz
Parteek Prasher
Kavita Pabreja
Nitin B Charbe
Harish Dureja
Shakti Dhar Shukla
Yusuf A Haggag
Murtaza M Tambuwala
Hamid A Bakshi
Kamal Dua
Deepak N Kapoor
Bahaa Al-Trad
Ritesh M Pabari
Poonam Negi
Ashref Ali K Dardouri
Alaa A A Aljabali
Mohammed Khan
Faruk L Hakkim
Salem Abobaker
Mateus Webba da Silva
Vijay Mishra
Meenu Mehta
Gaurav Gupta
Mohamed M Nasef
Dinesh Kumar Chellappan
Mazhar S Al Zoubi
Paul McCarron
Khalid M Al-Batanyeh
Journal: Cancers (2020)
Discipline: CLINICAL MEDICINE
Keywords: HIF-1α; albumin nanoparticles; colon cancer; nuclear P65; piceatannol.
Affiliations: Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Tanta, Tanta 31111, Egypt., Department of Basic Medical Sciences, Yarmouk University-Faculty of Medicine, Irbid 566, Jordan., School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab 144411, India., School of Pharmacy and Pharmaceutical Science, Ulster University, Coleraine, County Londonderry, Northern Ireland BT52 1SA, UK., Department of Gynecology, European Competence Center for Ovarian Cancer, Campus Virchow, Klinikum Charite-Universitatmedizin Berlin, augustenburger Platz 1, Berlin 13353, Germany., Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak 124001, India., Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi 110062, India., School of Pharmacy, Suresh Gyan Vihar University, Jagatpura, Jaipur 302017, India., Priority Research Centre for Healthy Lungs, Hunter Medical Research Institute (HMRI) and School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, New South Wales NSW 230, Australia., Department of Chemistry, University of Petroleum & Energy Studies, Dehradun 248007, India., School of Pharmaceutical Sciences, Shoolini University of Biotechnology and Management Sciences, Solan, India 173229, India., Department of Life Sciences, School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur 57000, Malaysia., School of Biomedical Sciences, University of Ulster, Coleraine, Northern Ireland, BT52 1SA, UK., Discipline of Pharmacy, Graduate School of Health, University of Technology, Sydney, NSW 2007, Australia., Centre for Inflammation, Centenary Institute, Sydney, NSW 2050, Australia 2308, Australia.

Abstract:  Piceatannol (PIC) is known to have anticancer activity, which has been attributed to its ability to block the proliferation of cancer cells via suppression of the NF-kB signaling pathway. However, its effect on hypoxia-inducible factor (HIF) is not well known in cancer. In this study, PIC was loaded into bovine serum albumin (BSA) by desolvation method as PIC-BSA nanoparticles (NPs). These PIC-BSA nanoparticles were assessed for in vitro cytotoxicity, migration, invasion, and colony formation studies and levels of p65 and HIF-1α. Our results indicate that PIC-BSA NPs were more effective in downregulating the expression of nuclear p65 and HIF-1α in colon cancer cells as compared to free PIC. We also observed a significant reduction in inflammation induced by chemical colitis in mice by PIC-BSA NPs. Furthermore, a significant reduction in tumor size and number of colon tumors was also observed in the murine model of colitis-associated colorectal cancer, when treated with PIC-BSA NPs as compared to free PIC. The overall results indicate that PIC, when formulated as PIC-BSA NPs, enhances its therpautice potential. Our work could prompt further research in using natural anticancer agents as nanoparticels with possiable human clinical trails. This could lead to the development of a new line of safe and effective therapeutics for cancer patients. 

Retracted: No

PubPeer comments:
 Figure 4C appears to include two images which overlap, or at least share the same source. *** ![file] , 
 Figure 2: Some panels appear to show overlapping areas. The rows show different conditions, the columns three different replicates, so all panels were expected to be different. ![file] , 
 All overlapping images have been corrected, these changes have been reviewed and approved by the Academic editors of Cancers Journal. The Journal will publish the revised images as corrections in due course. Revised Figure 2A. ![file] Revised Figure 2C ![file] Revised Figure 4C ![file] ![file] , 
 The correction has been published by Cancers Journal https: www.mdpi.com 2072-6694 12 12 3587 ,