MiR-101-3p Regulates the Viability of Lung Squamous Carcinoma Cells via Targeting EZH2
Authors: Xudong Xiang
Yulin Lu
Yunhe Ju
Yu Hou
Li Chang
Lan Li
Journal: Journal of Cellular Biochemistry (2017)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: EZH2; SQUAMOUS CELL CARCINOMA OF LUNG; miR-101-3p.
Affiliations: Department of Radiation Oncology, The Third Affiliated Hospital of Kunming Medical University, Cancer Hospital of Yunnan Province, Kunming 650118, P.R.China., Nursing School, Kunming Medical University, Kunming 650118, P.R.China., Department of Thoracic Surgery, The Third Affiliated Hospital of Kunming Medical University, Cancer Hospital of Yunnan Province, Kunming 650118, P.R.China.

Abstract:  The aim of this study was to investigate the effects of miR-101-3p on the viability, migration, invasion, and mitosis of lung squamous carcinoma cells by inhibiting EZH2. In this study, RT-qPCR was used to detect the expression of miR-101-3p and EZH2 in both tissues and cells at RNA level. The dual luciferase reporter gene system was used to determine whether there was targeting relationship between miR-101-3p and EZH2-3'UTR. Western Blot was used to detect the expression of EZH2 as well as the proliferation and invasion related proteins. The CCK-8 assay, Transwell invasion assay, wound healing assay and flow cytometry were conducted to test the cell viability, invasion, migration and apoptosis. The results of RT-qPCR and Western blot showed that miR-101-3p was low-expressed and EZH2 was overexpressed in lung squamous cell carcinoma tissues and cells. Meanwhile the Western blot confirmed the effects of EZH2 expression on the proliferation and invasion of carcinoma cells. The results of luciferase assay and RT-qPCR showed that miR-101-3p had a negative regulation effect on EZH2. The CCK-8 assay, Transwell invasion assay, wound healing assay and flow cytometry results showed that the inhibition of EZH2 or the up-regulation of miR-101-3p inhibited the viability, migration, invasion and cell cycle but promoted cell apoptosis of lung squamous cell carcinoma. MiR-101-3p could inhibit the viability, migration, invasion, and cell cycle of lung squamous carcinoma cells by inhibiting the EZH2. J. Cell. Biochem. 118: 3142-3149, 2017. © 2016 Wiley Periodicals, Inc. 

Retracted: No

PubPeer comments:
 Fig 3E.  statistical analysis of apoptosis and apoptotic rate of cells in different groups . I am not entirely sure how four flow-cytometry plots from four unrelated runs turn out to include areas of similarly-arranged cells. ![file] , 
 Duplication in figure 3C. Here is figure 3: ![file] Here a zoom in, duplicated panels in the pink box ![file] , 
 Some additional findings in Figure 3C: ![file] , 
 Fig 3C. The theory here is that the red peaks and the blue-striped  mesa in each panel are standard template histograms of cell-count against protein marker for populations of cells in the different mitosis phases, scaled until their sum fits the observed histogram of cell-count against marker. ![file] The templates should be the same shape in each panel (they aren t). The sum of the S-phase mesa (blue arrow) and the right-hand G2 M peak (red arrow) should at least vaguely match the observed black line (black arrow). Hint: they don t. ,