MicroRNA-142-3p, a new regulator of RAC1, suppresses the migration and invasion of hepatocellular carcinoma cells
Authors: Xin Li
Hua Tang
Xinghua Wang
Lifen Wu
Chunli Cai
Min Liu
Journal: FEBS Letters (2011)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: RAC1; microRNA-142-3p; gene expression; migration; invasion
Affiliations: Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China.

Abstract:  RAC1 regulates a diverse array of cellular events including migration and invasion. MicroRNAs (miRNAs) have a key role in the regulation of gene expression. In this study, we demonstrated that microRNA-142-3p (miR-142-3p) acted as a negative regulator of human RAC1. Overexpression of miR-142-3p decreased RAC1 mRNA and protein levels. Moreover, the overexpression of miR-142-3p suppressed, while blocking of miR-142-3p increased colony formation, migration and invasion in hepatocellular carcinoma (HCC) cell lines (QGY-7703 and SMMC-7721). RAC1 overexpression without the 3'untranslated region abolished the effect of miR-142-3p in the QGY-7703 and SMMC-7721 cells. These results demonstrated that miR-142-3p directly and negatively regulates RAC1 in HCC cells, which highlights the importance of miRNAs in tumorigenesis. 

Retracted: No

PubPeer comments:
 Fig 3D colony pictures more similar than would be expected ![file] , 
 Dear Sir, Thank you very much for you to point out the error in Fig. 3D. I apologize for this mistake. When I got your email, I immediately checked the original maunscripts and found that the current figure 3 were merged previous figure 3 (current figure 3A and 3B) and previous figure 4 (current figure 4C and 4D) in early version of manuscript. The previous figure 3 and 4 were attached below. It placed wrong images by mistake. I will asked the graduates (Lifen Wu, first author) for that and we will make the corrigendum. I apologize for this mistake due to our carelessness. The authors would like to apologize for any inconvenience caused. ![file] Best regards and Happy holidays, Hua Tang, Ph.D. ![file] ![file] ![file] , 
 Dear Lottia, Thank you very much for you to point out the error in Fig. 3D. I apologize for this mistake. When I got your email, I immediately checked the original maunscripts and found that the current figure 3 were merged previous figure 3 (current figure 3A and 3B) and previous figure 4 (current figure 4C and 4D) in early version of manuscript. The previous figure 3 and 4 were attached below. It placed wrong images by mistake. I will asked the graduates (Lifen Wu, first author) for that and we will make the corrigendum. I apologize for this mistake due to our carelessness. The authors would like to apologize for any inconvenience caused. Best regards and Happy holiday! Hua Tang, Ph.D. ![file] ![file] , 
 Dear Dr. Tang, can we ask for an update, please? Did you contact the journal for a corrigendum? I can t seem to find one. Also, could you also please check another possible duplication in Figure 4? I have marked it here below with green boxes. ![file] , 
 Expression of Concern (somehow marked as a  Correction ), June 14, 2021: https: febs.onlinelibrary.wiley.com doi 10.1002 1873-3468.14093  This Expression of Concern is for the above article, published online on 7 April 2011, in Wiley Online Library (wileyonlinelibrary.com), and has been published by agreement between the journal Editor-in-Chief, Michael Brunner, and John Wiley Sons Ltd. The Expression of Concern has been agreed due to concerns about data integrity raised by an independent source regarding a possible duplication in in figures 3D and figure 4A B. The authors institution, Tianjin Medical University, has been contacted and an investigation of this paper and several others from the group of Hua Tang is underway. While the conclusions of this investigation are awaited, the journal has decided to issue this Expression of Concern. , 
 Assessed here, as part of the second of 10 broad investigations: https: www.nsfc.gov.cn publish portal0 tab442 info85495.htm **Decisions on handling scientific research misconduct cases investigated and dealt with in 2022 (first batch)** Date 2022-05-13 Source: u3000 Author: u3000 u3010 Large, Middle and Small u3011 u3010Printu3011 u3010Closeu3011 u3000u3000The National Natural Science Foundation of China resolutely implements the spirit of the  Several Opinions on Further Strengthening the Construction of Scientific Research Integrity and  Opinions on Further Promoting the Spirit of Scientists and Strengthening the Construction of Style and Study Style by the General Office of the Central Committee of the Communist Party of China and the General Office of the State Council. Horizontal science and technology are self-reliant and self-reliant, continue to carry out scientific research integrity construction, and seriously investigate and deal with scientific research misconduct involving the National Natural Science Foundation of China. The Supervision Committee of the National Natural Science Foundation of China conducts investigations, verifications and comprehensive judgments on the accepted scientific research integrity cases, in accordance with the Regulations of the National Natural Science Foundation of ) and  Measures for Investigation and Handling of Scientific Research Misconduct in National Natural Science Foundation of China and other regulations and systems to propose handling suggestions. After deliberation at the second executive meeting of the National Natural Science Foundation of China in 2022, it was decided to deal with the relevant parties involved. The 10 handling decisions (online release version) that have been criticized and criticized are hereby announced. -------------------------------- **Decision on the handling of problems such as improper manipulation of images, confusion of experimental data, and false information in project applications, progress reports and final reports published by Tang Hua et al.** - National Science and Technology Financial Supervisory Office [2022] No. 2 The Supervision Committee of the National Natural Science Foundation of China launched an investigation into the papers published by Tang Hua and others of Tianjin Medical University for suspected academic misconduct. The papers involved are as follows: ... Paper 11:  Lifen Wu # , Chunli Cai # , Xinghua Wang # , Min Liu, Xin Li, Hua Tang*. **MicroRNA-142-3p, a new regulator of RAC1, suppresses the migration and invasion of hepatocellular carcinoma cells**. FEBS Letters . 2011, 585(9):1322-1330.u201d (marked with fund numbers 30873017, 91029714, 31071191) ... According to the investigation of the supporting unit, the above paper has problems such as improper operation of pictures and confusion of experimental data. The corresponding author Tang Hua is mainly responsible for the above problems. In addition, Tang Hua has included the above papers in his 7 National Natural Science Foundation of China projects (approval numbers 30873017, 91029714, 31071191, 31270818, 81572790, 91629302, 81830094) applications, progress reports, and final reports. Responsible for the objective results of false information in the project application, progress report or final report. u3000u3000After deliberation at the 12th meeting of the Fifth Session of the Supervision Committee of the National Natural Science Foundation of China (Life Medicine Professional Committee), and the approval of the 2nd Executive Meeting of the National Natural Science Foundation of China in 2022, it was decided to follow the thirty-fifth National Natural Science Foundation of China Regulations. Item 4 of Article 4, Item 2 of Article 16 and Item 3 of Article 17 of  Measures for Handling Misconduct in Science Fund Funding Work by the Supervision Committee of the National Natural Science Foundation of China (for Trial Implementation) , revocation of Tang Hua National Natural Science Foundation of China Science Fund Projects  Research on the molecular mechanism of Hsa-miR-10a inhibiting colon cancer metastasis (Grant No. 30873017),  Research on the interaction between miRNA and TNF-u03b1-IKK-NF-u03baB signaling pathway genes in ovarian cancer cells (Approval No. 91029714),  Research on the mechanism of miR-372 regulating the cycle of cervical cancer cells (Approval No. 31071191),  Regulation of Ago2 stability by PSMC3 and its mechanism (Approval No. 31270818),  miR-372 in liver cancer cells Epigenetic regulation and functional mechanism of 615-5pu201d (Grant No. 81572790), u201cThe mechanism of inflammatory factors activating NF-u03baB STAT3 to regulate the malignant behavior of tumor cells and the formation of pre-metastatic microenvironment through epigenetic modificationu201d ( Approval No. 91629302),  Study on the role and mechanism of m6A-RNA mediated by HPVE6 E7 in cervical cancer cells mediated by METTL3 (approval No. 81830094), recover the funds allocated for the above 7 projects, cancel the Tanghua National Natural Science Foundation of China The Science Foundation project is eligible for 5 years (January 18, 2022 to January 17, 2027), and the criticism will be given to Tang Hua. - National Natural Science Foundation of China - March 30, 2022 ,