XTP8 stimulates migration and invasion of gastric carcinoma through interacting with TGIF1
Authors: X Zhao
K Jia
M Xi
Q-H Wen
J-M Cheng
L Cheng
Journal: European review for medical and pharmacological sciences (2020)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: XTP8; TGIF1; gastric carcinoma; progression; expression characteristics
Affiliations: Department of General Surgery, Shanxi Cancer Hospital, Taiyuan, China. .

Abstract:  Objective: To determine expression characteristics of XTP8 and TGIF1 in gastric carcinoma (GC), and the potential roles of XTP8/TGIF1 axis in influencing the progression of GC. Materials and methods: The expression levels of XTP8 and TGIF1 in GC tissues and cells were detected. Their functions in prognosis in GC patients were evaluated by the Kaplan-Meier method. The correlation between the XTP8 level and the pathological indexes of the GC patients were analyzed. The changes in the proliferation, migration, and invasion capacities of MKN-45 and SGC-7901 cells affected by XTP8 and TGIF1 were assessed. The interaction between XTP8 and TGIF1 was determined through Dual-Luciferase reporter gene assay and rescue experiments. Results: XTP8 was upregulated in GC tissues and cells. XTP8 level was positively correlated with lymphatic and distant metastasis, as well as poor prognosis of GC patients. The silence of XTP8 attenuated proliferation, migration, and invasion capacities of MKN-45 and SGC-7901 cells. TGIF1 was the downstream gene binding to XTP8, which was downregulated in GC, and XTP8 negatively regulated the TGIF1 level in GC tissues. Importantly, the knockdown of TGIF1 could abolish the regulatory effect of XTP8 on GC cell behaviors. Conclusions: XTP8 is upregulated in GC and is closely linked to lymphatic metastasis, distant metastasis, and poor prognosis of GC patients. Besides, it accelerates the malignant progression via negatively regulating TGIF1. 

Retracted: No

PubPeer comments:
 This paper is part of a group of papers with a characteristic Kaplan Meier curve with a comb-like appearance. These plots all have a drop to 0 % survival at 75 months, a.k.a  Hoya s Law . Within the Comb papers, there is a subgroup of at least 9 papers that have very similar negative correlation plots. * X Shi et al., ERMPS (2019), DOI: 10.26355 eurrev_201912_19778 - https: pubpeer.com publications 3133C4267A6FBF81D00B0AF9FB9F2C * CY Yang et al., ERMPS (2019), DOI: 10.26355 eurrev_201908_18736 - https: pubpeer.com publications 04A58FD5AFBB311D1A2938B0612E1E * HG Zhang et al., ERMPS (2019), DOI: 10.26355 eurrev_201903_17239 - https: pubpeer.com publications D916E15502393940A4F3151E1D300C * JY Chen et al, ERMPS (2020), DOI: 10.26355 eurrev_202001_20040 - https: pubpeer.com publications 9266A736E004B8D7C32F6811161C1C * SS Li et al., ERMPS (2020) - DOI: 10.26355 eurrev_202002_20163 - https: pubpeer.com publications 5CDE9845135F510EC218243CD501F9 * TZ Zheng et al., ERMPS (2020) - DOI: 10.26355 eurrev_202002_20369 - https: pubpeer.com publications AF0159381E48D7937D78C948FAB379 * JB Ma et al., ERMPS (2019) - DOI: 10.26355 eurrev_201902_17108 - https: pubpeer.com publications 2EB1B6A17E9877F2D7F8FE4E117446 * HW Sun et al., ERMPS (2019)- DOI: 10.26355 eurrev_201910_19299 - https: pubpeer.com publications 937B0CAD1A01B66E854F4A6CCDA56B * K Jia et al., ERMPS (2020) - DOI: 10.26355 eurrev_202003_20508 - https: pubpeer.com publications 11550517E8A0503AFDC49D460BAD30 And here is a collage of the nine correlation plots, where I have circled some similar groupings of points. ![file] , 
 #1 Figs 1C and 4C.  Prognosis in GC patients with high and low expression levels of XTP8 ...  Prognosis in GC patients with high and low expression levels of TGIF1 . When patients are dichotomised by TGIF1 expression there are far more survivors after 75 months than if they are dichotomised by XTP8. This is important clinical news. ![file] , 
 A panel in Figure 2C from this paper appears to show an overlap with a panel in Figure 4D of L Yang et al., ERMPS (2020), DOI: 10.26355 eurrev_202002_20156 - https: pubpeer.com publications B324F0C5CC90125D4A9AA9F883A5DC Shown with red boxes. ![file] , 
 #1 Fig 3D.  A negative correlation between XTP8 and TGIF1 in GC tissues . ![file] At right, a superimposition of 10 variants from various papers in the same journal. ,