Retracted : Down‐regulation of the long noncoding RNA‐HOX transcript antisense intergenic RNA inhibits the occurrence and progression of glioma
Authors: Hua-Chao Yang
Jun-Yi Wang
Bang-Qing Wang
Zhen-Yu Wei
Xing-Yao Bu
Bin Yang
Journal: Journal of Cellular Biochemistry (2018)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: RNA-HOX transcript antisense intergenic RNA; cell apoptosis; cell invasion; cell migration; cell proliferation
Affiliations: Department of Neurosurgery, People's Hospital of Zhengzhou University, Zhengzhou, P.R. China., Department of Neurosurgery, The 371th Central Hospital of Chinese People's Liberation Army, Xinxiang, P.R. China., Department of Neurology, Henan University of Chinese Medicine, Zhengzhou, P.R. China.

Abstract:  This study aims to explore the role of long noncoding RNA (lncRNA)-HOX transcript antisense intergenic RNA (HOTAIR) in the occurrence and progression of glioma. Fresh glioma and normal brain tissues were classified into a glioma group (n = 67) and a normal group (n = 64) respectively. U87 cells were assigned into the blank, sh-NC, and sh-HOTAIR groups. Quantitative real-time polymerase chain reaction (qRT-PCR) was utilized to determine HOTAIR expression. Cell proliferation, cell cycle and cell apoptosis rates were detected by cell counting kit-8 (CCK-8) and flow cytometry (FCM). Scratch test and transwell assay were conducted for cell migration and invasion. Orthotopic glioma tumor model in nude mice was established by inoculating tumor cell suspension. Hematoxylin-Eosin (HE) staining was used to observe the growth and invasion of orthotopic glioma tumors. The expression of HOTAIR and cell viability was found to be lowest in the sh-HOTAIR group among the three groups. The sh-HOTAIR group exhibited a higher apoptotic rate and lower number of cell migration compared with the blank and sh-NC groups. Additionally, the speed of wound healing was slower, the migration distance decreased and the survival time of nude mice was extended in the sh-HOTAIR compared to the other groups. Moreover, the sh-HOTAIR group demonstrated reduced lesion sizes and inflammation, no convulsions or hemiplegia and lesser number of satellite metastases. Our findings support that down-regulation of HOTAIR could inhibit cell proliferation, promote cell apoptosis as well as suppress cell invasion and migration in the progression of glioma. 

Retracted: Yes

PubPeer comments:
 Fig 4A.  Differences in Cell cycle patterns of glioma cells in the blank, sh-NC and sh-HOTAIR groups Note: A, cell cycle distribution of the three groups using FCM . These histograms appear to have been hand-drawn. They are not healthy. ![file] , 
 Retracted [30 June 2021] . ------------------------------- The above article, published online on 31 August 2017 in Wiley Online Library has been retracted by agreement between the the journal s Editor in Chief, Prof. Dr. Christian Behl, and Wiley Periodicals LLC. The retraction has been agreed following an investigation based on allegations raised by a third party. As several flaws and inconsistencies between results presented and experimental methods described were found, the editors consider the conclusions of this article to be invalid. ---------------------------------- UPDATED: One more comparison, for dead-horse-flogging-purposes: *[above]* Fig 9A,  satellite metastases in each group using HE staining . *[below]* Fig 2 from [Han et al (2018)] ,  Hematoxylin-eosin staining for neurons in rats from the normal, sham and model groups . Flipped through 180 deg for ease of comparisons. ![file] ,