miR-451a Inhibited Cell Proliferation and Enhanced Tamoxifen Sensitive in Breast Cancer via Macrophage Migration Inhibitory Factor
Authors: Liming Zhou
Tianyu Miao
Zhenru Liu
Ting Feng
Mingyuan Li
Zhouhua Jiang
Hong Li
Journal: BioMed Research International (2015)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: microRNA-451a; cell proliferation; tamoxifen; breast cancer cells; lentiviral vectors
Affiliations: Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China, Department of Pharmacology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, China, West China School of Medicine, Sichuan University, Chengdu 610041, China, 985 Science and Technology Platform for Innovative Drugs of Sichuan University, Chengdu 610041, China

Abstract:  This study aims to investigate the regulative effects of microRNA-451a (miR-451a) on cell proliferation and sensitivity to tamoxifen in breast cancer cells. In cell culture experiments, the lentiviral vectors of pHBLV-miR-451a and pHBLV-miR-451a sponge were constructed and used to transfect MCF-7 and LCC2 cells. The transfection efficiency was tested by fluorescent observation, and cell lines with stable over- or downregulated expression of miR-451a were established. The expression of miR-451a and the target gene macrophage migration inhibitory factor (MIF) were detected by real-time reverse transcriptase polymerase chain reaction and/or western blot. Moreover, MTT assay, colony formation, and Transwell invasion assays were also performed. Data showed that the recombinant lentiviral vectors were constructed correctly, and the virus titer was 1 × 10(8) CFU/mL. The stable transfected cells were obtained. Overexpression of miR-451a downregulated MIF expression in mRNA and protein levels and inhibited cell proliferation, colony formation, and invasion of breast cancer cells. Downregulation of miR-451a upregulated MIF expression and increased breast cancer cell growth, invasion, and tamoxifen sensitivity. In summary, the miR-451a/MIF pathway may play important roles in the biological properties of breast cancer cells and may be a potential therapeutic target for breast cancer. 

Retracted: Yes

PubPeer comments:
 Figure 5B. * Red boxes: The LCC2 Lv-miR-451a NC and LCC2 Lv-miR-451a panels appear to look similar albeit with a different field of focus. ![file] , 
 Figure 7A. * Pink boxes: The top and bottom GAPDH panels look remarkably similar to each other if one of the two is rotated 180 degrees. Pink arrows highlight the direction. ![file] , 
 Figure 8A: * Blue boxes: The 0h MCF-7 and LCC2 panels appear to share an area of overlap, with one of the two panels shifted and slightly rotated. ![file] , 
 Figure 8B. * Red boxes: the siMIF panel shown here looks very similar to two panels in Figure 5B marked above in comment #1. ![file] , 
 Retraction, 1 July 2022: https: www.hindawi.com journals bmri 2022 9758629  BioMed Research International has retracted the article titled u201cmiR-451a Inhibited Cell Proliferation and Enhanced Tamoxifen Sensitive in Breast Cancer via Macrophage Migration Inhibitory Factoru201d [1], because several images were found to be duplicated as follows: * Figure 5(b) LCC2 Lv-miR-451aNc panel is identical to both the Figure 5(b) LCC2 Lv-miR-451a panel and the Figure 8(b) siMIF panel. * Figure 7(a) MCF7 GAPDH lanes are a rotated version of the Figure 7(a) LCC2 GAPDH lanes. * Figure 8(a) LCC2 0u2009h panel is identical to the Figure 8(a) MCF-7 0u2009h panel. The authors responded to explain that the errors were introduced during manuscript preparation. However, due to the number of duplicated images the Editorial Board no longer consider the data to be reliable. The article is therefore retracted with the agreement of the editorial board. The authors agree with this retraction. ,