Long noncoding RNA LINC00052 inhibits colorectal cancer metastasis by sponging microRNA‐574‐5p to modulate CALCOCO1 expression
Authors: Zhengshu Liu
Dongmei Xiong
Yongguo Li
Hua Tang
Ke Chen
Gangfeng Yu
Journal: Journal of Cellular Biochemistry (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: LINC00052; colorectal cancer; invasion; miR-574-5p; migration.
Affiliations: Department of Infectious Diseases, Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China., Nursing Department, Chongqing Medical and Pharmaceutical College, Chongqing, China., Department of Physical Examination, he First Affiliated Hospital of Chongqing Medical University, Chongqing, China., Department of Forensic Medicine, Chongqing Medical University, Chongqing, China.

Abstract:  The dysregulation of long-chain noncoding ribonucleic acid (lncRNA) is a common phenomenon in many human cancers. Some studies on the biological function of long intergenic non-protein-coding RNA 52 (LINC00052) in cancer indicate that this gene can act as either oncogene or tumor suppressor in some kinds of cancers, such as breast cancer, gastric cancer, liver cancer, and lung cancer. However, the biological function of LINC00052 in colorectal cancer (CRC) has not been studied. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and Western blot (WB) techniques were applied to detect the expression levels of LINC00052, miR-574-5p, and calcium-binding and coiled-coil domain 1 (CALCOCO1) in CRC cells and tissues. We authenticated the biological function of LINC00052 and miR-574-5p in CRC, and find some target genes for LINC00052 and miR-574-5p via bioinformatics methods. Dual-luciferase reporter gene assay was performed to identify the interaction between LINC00052 and miR-574-5p or CALCOCO1 and miR-574-5p. The results demonstrated that LINC00052 was downregulated in CRC tissues compared with their adjacent tissues. And LINC00052 could suppress CRC cells metastasis both in vivo and in vitro. Beyond that, miR-574-5p was upregulated in CRC tissues, and as an oncogene, it accelerated CRC cell migration and invasion. More importantly, the results of our research demonstrated that LINC00052 could regulate the expression of CALCOCO1 via sponging miR-574-5p in CRC. Overall, our study illuminated the lncRNA-miRNA functional networks in CRC, and these results might provide a new research direction for the diagnosis and treatment of CRC. 

Retracted: Yes

PubPeer comments:
 Brought to my attention by a reader. Figure 1C and 3D: Two panels that are labeled as different experiments appear to be showing an overlap. Shown with red ellipses. Note that the image supposed to be showing the bottom of a well, but the photos are shifted. Could the authors please clarify? ![file] , 
 #1 ![file] Dear Sir, Thank you very much for your comments. Yes, you are quite right, these two Figs are overlap in two experiments. This is a error that can not be forgived. I have responsibility for this error. I am sorry to all readers. After the first authour checked her original data, she corrected these data as following. And I will contact the eitor of Journal of Cellular Biochemistry and correct our data. Thank you very much for your help. Sinncerely, Hua Tang 10 06uff0c 2020![file] ![file] , 
 Is there any word from the editors about the proposed correction? , 
 #3 Dear Siruff0c I have written a mail to the editor of JCB Journal and he will contact with me soon. The following is what he say. Dear Dr.Tang, Thank you very much for your e-mail. I have forwarded your request to the production team, they would contact you very soon. Uma Maheswari Editorial Assistant Journal of Cellular Biochemistry , 
 #4 Thank you, Dr Tang! , 
 Retracted [23 May 2022] . ---------------------------- The above article, published online on 19 May 2019 in Wiley Online Library , has been retracted by agreement between the authors, the journal s Editor in Chief, Prof. Dr. Christian Behl, and Wiley Periodicals LLC. The retraction has been agreed after the authors asked to correct the article. An investigation revealed several flaws and inconsistencies between results presented and experimental methods described. Thus the editors consider the conclusions of this article to be invalid. ,