S100A16-induced adipogenesis is associated with up-regulation of 11 β-hydroxysteroid dehydrogenase type 1 (11β-HSD1)
Authors: Jie Yang
Yun Liu
Dong Li
Shan Lu
Jing Bao Kan
Ling Xi
Xiubing Liang
Rihua Zhang
Pei Tong
Dongming Su
Journal: Bioscience reports (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: 11β-HSD1; S100A16; adipogenesis; obesity; type 2 diabetes.
Affiliations: Department of Geratology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China., The Center of Metabolic Disease Research, Nanjing Medical University, Nanjing 210029, China., Department of Orthopedics, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China., Department of Geratology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China .

Abstract:  The steadily increasing epidemic of obesity continues at alarming rates, is an important public health problem, and expression changes of S100A16 and 11 β-hydroxysteroid dehydrogenase type 1(11β-HSD1) is attributable to the adipocyte differentiation. In our previous study, we found that 11β-HSD1 protein expression increased in S100A16-overexpressed 3T3-L1 cell model. In order to further investigate the relationship between S100A16 and 11β-HSD1, and the molecular mechanisms of S100A16-induced adipogenesis, we constructed S100A16 transgenic and knockout mouse, and S100A16-overexpressed 3T3-L1 preadipocyte cell. Using S100A16 transgenic (S100A16Tg/+) mice fed with normal fat diet (NFD) and high fat diet (HFD) diet model, we evaluated the effect of S100A16 on adipogenesis, expression of 11β-HSD1, and RNA sequencing and quantification of gene expression. Using the 3T3-L1 cell model, we examined the effect of S100A16 and 11β-HSD1 on pre-adipocyte differentiation, and cell signaling events of 11β-HSD1 overexpression induced by S100A16. We found that when compared with C57BL/6 mice, overexpression of S100A16 under the condition of HFD increased lipid content in WAT and fat infiltration in hepatocytes, 11β-HSD1 protein expression increased along with S100A16. Elevated S100A16 and 11β-HSD1 expression promoted adipogenesis in 3T3-L1 cells. Overexpression of S100A16 inhibited the degradation of 11β-HSD1. We conclude that S100A16-induced adipogenesis is associated with up-regulation of 11β-HSD1. 

Retracted: No

PubPeer comments:
 I posed a question about this paper on social media after noting some repeated numbers in the log2 ratio column. The authors may wish to check that column for errors. One example shown below. However, one other reader replied, u201dThe entirety of both tables is questionable. 1st each measurement must involve error. Where are the errors? Why transform values using log base 2? The *measured values* should be presented not log2 values. If the P values were determined using log2 numbers, the P values are wrong.u201d Could the authors respond to this comment please? *** ![file] , 
 They are two different genes. When they compared it to the S100A16, the difference was almost the same.This is not a mistake. , 
 #2 We knew the same results during the experiment. And this was the data given by the calculation software. We can not changed it. The original data is attached to this message.![file] The p value has nothing to do with the log2 Ratio. The p value is calculated using the hypothesis test.! ,