Overexpression of long intergenic noncoding RNA LINC00312 inhibits the invasion and migration of thyroid cancer cells by down-regulating microRNA-197-3p
Authors: Kai Liu
Qing Luo
Wen Huang
Xiang Min
Dan-Qing Yan
Journal: Bioscience Reports (2017)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Cell migration; Long intergenic noncoding RNA LINC00312; MicroRNA-197-3p; Thyroid cancer; invasion and migration
Affiliations: Department of Otorhinolaryngology and Head and Neck Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, P.R. China., Department of Blood Transfusion, The First Affiliated Hospital of Nanchang University, Nanchang 330006, P.R. China., Department of Otorhinolaryngology and Head and Neck Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, P.R. China .

Abstract:  The study evaluated the ability of long intergenic noncoding RNA LINC00312 (LINC00312) to influence the proliferation, invasion, and migration of thyroid cancer (TC) cells by regulating miRNA-197-3p. TC tissues and adjacent normal tissues were collected from 211 TC patients. K1 (papillary TC), SW579 (squamous TC), and 8505C (anaplastic TC) cell lines were assigned into a blank, negative control (NC), LINC00312 overexpression, miR-197-3p inhibitors, and LINC00312 overexpression + miR-197-3p mimics group. The expression of LINC00312, miR-197-3p, and p120 were measured using quantitative real-time PCR (qRT-PCR) and Western blotting. Cell proliferation was assessed via CCK8 assay, cell invasion through the scratch test, and cell migration via Transwell assay. In comparison with adjacent normal tissues, the expression of LINC00312 is down-regulated and the expression of miR-197-3p is up-regulated in TC tissues. The dual luciferase reporter gene assay confirmed that P120 is a target of miR-197-3p The expression of LINC00312 and p120 was higher in the LINC00312 overexpression group than in the blank and NV groups. However, the expression of miR-197-3p was lower in the LINC00312 overexpression group than in the blank and NC groups. The miR-197-3p inhibitors group had a higher expression of miR-197-3p, but a lower expression of p120 than the blank and NC groups. The LINC00312 overexpression and miR-197-3p inhibitor groups had reduced cell proliferation, invasion and migration than the blank and NC groups. These results indicate that a LINC00312 overexpression inhibits the proliferation, invasion, and migration of TC cells and that this can be achieved by down-regulating miR-197-3p. 

Retracted: No

PubPeer comments:
 Figure 5 appears to have overlapping images that are stretched somehow? For images that are putatively of different cell lines. ![file] , 
 Fig 3B. I have singled out the actin loading control, enhancing its contrast. ![file] It has appeared in a number of other papers by unrelated teams of authors. Fig 9B of [Han et al (2018)] : ![] Fig 5B of [Wu et al (2018)] . ![] , 
 #1  Figure 5 appears to have overlapping images that are stretched somehow? For images that are putatively of different cell lines. Here I have excerpted those panels for comparison with a composite, at upper right, constructed from overlapping panels of *[below]* Fig 7A of [Xiong et al (2018)] . ![file] Black, yellow, purple, green boxes mark other overlaps between panels of Fig 5 and 7A. 90-deg rotations involved. Fig 7A in turn overlaps with Fig 5F H of [Xie et al (2017)] , meaning that the latter also overlaps with the present Fig 5. ![] ![] ![] , 
 10.1042 BSR-20170109_EOC The Editorial Office has been made aware of potential issues surrounding the scientific validity of this paper, hence has issued an expression of concern to notify readers whilst the Editorial Office investigates. , 
 Retracted [8 June 2021] . --------------------------------------------- This article is being retracted from Bioscience Reports at the request of the Editor-in-Chief and the Editorial Board following receipt of a notification from a reader, alerting the Editorial Board to duplications in Figure 5, which also overlap with images published in Journal of Cellular Biochemistry (10.1002 jcb.26763) and in Bioscience Reports (10.1042 bsr20160542). The authors have been contacted in regards to the retraction, and have not responded to the Journal s queries and the concerns raised. Given the extent of the issues raised, the Editorial Board stand by the decision to retract the article. ,