Physcion 8-O-β-glucopyranoside prevents hypoxia-induced epithelial-mesenchymal transition in colorectal cancer HCT116 cells by modulating EMMPRIN
Authors: Z Ding
H Wu
P Jiang
J Tang
F Xu
Z Tang
G Li
Journal: Neoplasma (2016)
Discipline: CLINICAL MEDICINE
Keywords: EMMPRIN.; EMT; colorectal cancer; hypoxia; physcion 8-O-β-glucopyranoside.

Abstract:  Epithelial-mesenchymal transition (EMT) is considered as the most important mechanism that underlies the initiation of cancer metastasis. Here we report that Physicon 8-O-β-glucopyranoside (PG), a major active ingredient from a traditional Chinese herbal medicine Rumex japonicus Houtt, is capable of preventing human colorectal cancer cells from hypoxia-induced EMT. The treatment of the cells with PG reversed the EMT-related phenotype that has the morphological changes, down-regulation of E-cadherin, and hypoxia-induced cell migration and invasion. The effect was mediated at least in part by inhibiting the mRNA and protein expressions of EMMPRIN via modulation of PTEN/Akt/HIF-1α pathway. In addition, we found that PG-mediated prevention of EMT involved blockade of the hypoxia-induced up-regulation of Snail, Slug and Twist. In summary, this study showed that PG can prevent EMT induced by hypoxia, the environment that commonly exists in the center of a solid tumor. Given the low toxicity of PG to the healthy tissues, our study suggests that PG can serve as a safe therapeutic agent for suppressing cancer metastasis. 

Retracted: No

PubPeer comments:
 Fig 3F, 5B. Extra band inserted into 5B to illustrate the similarity of beta-actin control bands. ![file] , 
  Figure 1. PG suppresses hypoxia-induced migration ... in HCT116 cells . Below: Fig 4A from [Wang et al (2016)] .  PG suppresses migration ... of HepG2 cells . ![file] Some repeating panels marked, before I ran out of colors. Some repetitions involve a 180-deg rotation. Note that these are non-overlapping teams of authors, from different cities, so the source of the reuse is a mystery. , 
 Below: Fig 2,  PG prevents hypoxia-induced EMT. HCT116 cells are treated for PG at indicated concentration for 24 hours in hypoxia before assays are performed. (A) The representative graphs of the u03b2-catenin translocation determined by immno fluorescent staining were displayed . Above: Fig 2,  Effect of PG on EMT of MDAu2010MBu2010231 cells. MDAu2010MBu2010231 cells were incubated with PG at indicated concentration for 24h before the assay was performed. A. PG significantly inhibited the translocation of u03b2u2010catenin. From [Chen et al (2017)] . ![file] Could the authors comment on the reuse of one image? , 
 #1 Fig 3F again. This time compared to Fig 6D from [Xie et al (2015)] .  Western blot analysis to show the effect of hispidulin on:... E-cadherin expression in response to hispidulin, IGF-1 and LY294002. ... D. HT-29 cells were grown under hypoxia in the presence or absence of LY294002 then treatment with IGF-I (10 ng ml) for 2 hours. ![file] Perhaps the authors of the earlier paper are better-placed to describe exactly how the cells were treated, and what proteins were studied. , 
 #3 Above here, Fig 2A:  PG prevents hypoxia-induced EMT. HCT116 cells are treated for PGat indicated concentration for 24 hours in hypoxia before assays are performed. (A) The representative graphs of the u03b2-catenin translocation determined by immno fluorescent staining were displayed. Below: Fig 3B from[ Xie et al (2015)] .  Hispidulin prevents hypoxia-induced EMT. HT-29 cells growing in hypoxia were treated with hispidulin (25 u03bcM) for 24 hours. ... B. The effect of hispidulin on the expression of E-cadherin examined by florescence staining . ![file] Could the authors comment on the similarity of two panels (after 180 deg rotation), despite different descriptions? , 
 #3 And **another** sighting! Upper left, Fig 2 from [Chen et al (2017)] ; lower left, Fig 2. At right, Fig 3A from [Gao et al (2016)] :  PTTG overexpression promotes EMT process. A. Effect of PTTG overexpression on cell morphology was evaluated by fluorescent microscopy . ![file] These are very versatile cells. , 
 #4 Extended version - now including Fig 4B from [Han et al (2016)] at right.  Involvement of SOX2 in the modulatory effect of physcion on EMT. SW620 cells were incubated with 5 u03bcmol L physcion for 24 h....(B) Silencing SOX2 suppressed EMT, and the suppressive effects of physcion on EMT was significantly abrogated in SOX2-overexpressing SW620 cells.  ![file] , 
 Wuxi Maternity and Child Health Hospital announced the results of an investigation, on [14 September 2021](http: www.wxfuyou.com keyanchengxin kycx 4884.html). Thanks to Google Translate for the translation. -------------------------- In order to continue to carry out scientific research integrity construction, severely punish violations, standardize scientific research process management , and create a good environment for scientific and technological innovation, we hereby report the relevant paper issues recently investigated and dealt with by our unit as follows. The paper  Physcion 8-O-u03b2-glucopyranoside prevents hypoxia-induced epithelial-mesenchymal transition in colorectal cancer HCT116 cells by modulating EMMPRIN) published by Xu Fei, the original laboratory department of Wuxi Maternity and Child Health Hospital as the corresponding author and Ding Zhongyang, Wuxi Traditional Chinese Medicine Hospital as the first author.  , after investigation, the paper was commissioned by a third party to write and submit. Xu Fei was sentenced to three years in prison for other economic cases in 2017 , and Wuxi Maternity and Child Health Hospital expelled him in 2018 . The Wuxi Hospital of Traditional Chinese Medicine has dealt with relevant personnel as follows: give Ding Zhongyang scientific research admonitions , recover scientific research awards issued, cancel various types of science and technology projects (special projects, funds, etc.), various talent titles, and professional and technical job promotions, etc. Qualification for 5 years. ,