MiRNA-584 suppresses the progression of NK/T-cell lymphoma by targeting FOXO1
Authors: C-C Wang
X-Y Ying
L Han
Y-H Hou
Journal: European review for medical and pharmacological sciences (2020)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: miRNA-584; NK/T-cell lymphoma; proliferative abilities; invasive abilities; FOXO1
Affiliations: Hemato-Oncology, Minhang Hospital, Fudan University, Shanghai, China. .

Abstract:  Objective: This study aims to clarify the influences of miRNA-584 on proliferative and invasive abilities in NK/T-cell lymphoma, and to illustrate the underlying mechanism. Patients and methods: MiRNA-584 levels in peripheral blood of NK/T-cell lymphoma patients and healthy controls were detected by qRT-PCR. Potential relationship between miRNA-584 level and clinical indicators of NK/T-cell lymphoma patients was analyzed. Kaplan-Meier curves were depicted for assessing the prognostic value of miRNA-584 in NK/T-cell lymphoma patients. After overexpression or knockdown of miRNA-584, changes in proliferative and invasive abilities in KHYG-1 and SNK-6 cells were assessed by CCK-8 and transwell assay, respectively. The potential mechanism of miRNA-584 and its downstream gene FOXO1 in regulating the development of NK/T-cell lymphoma was explored by Dual-Luciferase reporter gene assay and rescue experiments. Results: Peripheral blood levels of miRNA-584 were lower in NK/T-cell lymphoma patients than those of healthy control. Compared with NK/T-cell lymphoma patients expressing high level of miRNA-584, those expressing a low level suffered worse tumor staging, higher rate of bone marrow invasion, and worse prognosis. Overexpression of miRNA-584 suppressed proliferative and invasive abilities in KHYG-1 and SNK-6 cells. On the contrary, knockdown of miRNA-584 yielded the opposite results. FOXO1 was showed to be the direct target binding miRNA-584, and its level was negatively regulated by miRNA-584. Notably, FOXO1 could partially abolish the regulatory effects of miRNA-584 on proliferative and invasive abilities in NK/T-cell lymphoma. Conclusions: Low level of miRNA-584 is closely linked to advanced stage, susceptibility to bone marrow invasion, and poor prognosis in NK/T-cell lymphoma. MiRNA-584 suppresses proliferative and invasive abilities in NK/T-cell lymphoma by targeting FOXO1. 

Retracted: No

PubPeer comments:
 Figure 2C: * Yellow boxes: A panel in this paper appears to overlap with a panel in a paper by different authors, where it represents glioma cells. That paper is: WJ Wang et al., doi: 10.26355 eurrev_202001_20056 - https: pubpeer.com publications A93964B21F3EE8BAC2E56B992DA8F9 ![file] , 
 Fig 1C.  Overall survival in NK T-cell lymphoma patients expressing high or low level of miRNA-584 . ![file] It is another manifestation of  Hoya s Law for J.BUOn ERMPS :  When 40 or 50 cases of any given cancer are dichotomised into half-groups of high- or low-expression of any given noncoding RNA, survival rate for one half-group or both always drops to 0% at 75 months. , 
 *[left]* Fig 3E from [Wang et al (2020a)] .  A negative correlation between peripheral blood levels of FOXO1 and miRNA-584 in NK T-cell lymphoma patients . *[right]* Fig 3F from [Wang et al (2020b)] .  A negative correlation was observed between the expression levels of HANR and miR-335 in glioma tissues . ![file] Implausibly many points in common. For comparison:  Here is a small collage of the plots (h t [Elisabeth Bik] ): ![] Here, an overlay of 20 correlation scatterplots from other papers (all based on the same spreadsheet), emphasising the points frequently occurring between them. ![file] ,