Effect of long non-coding RNA Gas5 on proliferation, migration, invasion and apoptosis of colorectal cancer HT-29 cell line
Authors: Hai-Juan Xiao
Han-Juan Xiao
Cheng-Gong Zhang
Jun-Ming Hou
Jing-Dong He
Jin Li
Yuan Wang
Jun-Ming Hu
Journal: Cancer Cell International (2018)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Apoptosis; Colorectal cancer; HT-29; Invasion; Long non-coding RNA growth arrest-specific transcript 5
Affiliations: Department of Oncology, Huai'an First People's Hospital, Nanjing Medical University, No. 6, Beijing Road West, Huai'an, 223300 People's Republic of China., Department of Oncology, Nanjing Medical University, Nanjing, 211166 People's Republic of China.

Abstract:  Objective: This study aims to investigate the effect of long non-coding RNA (lncRNA) Gas5 on proliferation, migration, invasion and apoptosis of colorectal cancer (CRC) HT-29 cell line. Methods: CRC and normal tissues were collected and prepared from a total of 126 CRC patients, and normal intestinal epithelial cell line FHC and CRC cell lines (HCT-8, HT-29, HCT-116 and SW-480) were prepared. Gas5 expression was detected by quantitative reverse transcriptase-polymerase chain reaction. HT-29 cell line exhibiting the lowest Gas5 expression was selected for further experimentation and divided into blank, negative control and pcNDA-Gas5 groups. The cell counting kit-8 assay was used to test cell proliferation. Flow cytometry was applied to examine cell apoptosis. Transwell assay was performed to detect the migration and invasion of HT-29 cells. The mRNA and protein expression of factors in the classical proliferation (Akt/Erk) and apoptosis (caspase-9/caspase-3) pathways were detected. Results: Gas5 expression was lower in CRC tissues compared to the adjacent normal tissues, and is also lower in CRC cell lines than FHC cell line. Gas5 expression was associated with tumor size and TNM staging. Gas5 expression, distant metastasis, tumor differentiation and TNM staging were independent CRC prognostic factors. The results showed that elevated Gas5 expression inhibited proliferation, migration and invasion, but promoted apoptosis of CRC cells. Meanwhile, elevated Gas5 expression inhibited mRNA expression of Akt and Erk and protein expression of p-Akt and p-Erk, which promoted Casp9 mRNA and pho-Casp9 protein expression but inhibited Casp3 mRNA and pho-Casp3 protein expression. Conclusion: The findings indicated that overexpression of lncRNA Gas5 can inhibit the proliferation, migration and invasion but promote apoptosis of CRC cells. 

Retracted: No

PubPeer comments:
 I don t understand why the surgery performed on rats was so elaborate and destructive when the goal of the experiment was simply to test whether exposure to the coordination polymers would hinder the post-surgical presence of *S. aureus*. Surely a spinal incision alone would suffice.  This **preformation** was carried out totally under the instructionsu2019 guidance with slight modifications. Briefly, the SD rats (180u2013220 g) utilized in this present study were obtained from the Nanjing University, and they were placed at 20u201325 C standard condition, with free food and water. All the **preformation** in this present research was approved by the Animal Ethics Committee of Nanjing University. Before the experiment, the rats were divided into the control group, model group, compound 1 treatment group and compound 2 treatment group (n = 18). Healthy rats were anesthetized by chloral hydrate (350u2013400 mg kg body weight) or pentobarbital sodium (with the body weight of 50u201360 mg kg). **The spine stereotaxic instrument fixes the skull and the transverse processes on both sides of T 10 and L1, and the skin of the operation area is disinfected. Bite off the T11 * T13 lamina, perform vertebral arch and vertebral osteotomy from the level of T12 * L1, adjust the stereotaxic device to retract the longitudinal axis of the spinal cord into the spinal canal and cause injury**. After the model construction, the two compounds were injected into the animals for the treatment at 5 mg kg concentration. Ethical permission to mutilate rats was apparently provided not by the researchers institution, but by the company that sold them. Is this normal? The curious term  preformation , in this current sense of  performance , only appears in [the products of a prolific coordination-polymer papermill] . ![] , 
 Papers in this curious genre tend to recycle a small list of incongruous, irrelevant References.  In the past few years, the design and establishment of the coordination polymers (CPs) on the basis of multiple organic ligands and clusters or metal ions have received extensive attention owing to their unique topological structure and the latent applications, for instance, gas separation and storage, luminescence, and magnetism, biomedicine, sensing materials as well as catalysis [3u20138]. - [7]. C. Y. Pei, Y. G. Chen, L. Wang, W. Chen, and G. B. Huang (2021). Appl. Surf. Sci. 535, 147682. ** Step-scheme WO3 CdIn2S4 hybrid system with high visible light activity for tetracycline hydrochloride photodegradation **  Nevertheless, the choice and design of appropriate organic ligands are still the key to construct CPs with ideal performances and structures [9u201312]. - [9]. N. Jiang, Y. Liu, X. N. Yu, H. B. Zhang, and M. M. Wang (2020). Int. J. Electrochem. Sci. 15, 5520. ** Corrosion Resistance of Nickel-Phosphorus Nano-ZnO Composite Multilayer Coating Electrodeposited on Carbon Steel in Acidic Chloride Environments  ** ,