MicroRNA-613 targets FMNL2 and suppresses progression of colorectal cancer
Authors: Bai Li
Zhihong Li
Zhongshi Xie
Bo Li
Si Chen
Journal: American journal of translational research (2016)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: FMNL2; colorectal cancer; miR-613; progression.
Affiliations: Department of Colorectal and Anal Surgery, The First Hospital of Jilin University Changchun 130021, P. R. China., Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University Changchun 130033, P. R. China., Department of Thoracic Surgery, The First Hospital of Jilin University Changchun 130021, P. R. China.

Abstract:  Increasing evidence indicates that dysregulation of miRNAs is involved in the initiation and progression of colorectal cancer (CRC). MicroRNA (miR)-613 has been reported to function as a tumor suppressor in many cancers. However, the precise role of miR-613 in CRC progression is unclear. This study aimed to investigate the role and underlying mechanism of miR-613 in growth and metastasis of CRC. Real-time quantitative PCR (qPCR) and western blot techniques were used to assess expression of miR-613 and formin-like 2 (FMNL2) in CRC cell lines and tissues. Luciferase reporter assays were conducted to investigate the association between miR-613 and FMNL2. Proliferation, wound healing, and transwell invasion assays, as well as flow cytometric analysis, were performed to evaluate the effect of miR-613 on proliferation, migration, invasion, and cell-cycle status, respectively, of CRC cells. We found that miR-613 was significantly downregulated in CRC cell lines and tissue samples, and correlated closely with TNM stage. miR-613 suppressed CRC cell proliferation, migration, and invasion, and induced cell-cycle arrest at G1 phase. FMNL2 was identified as a direct target of miR-613 in CRC cells. Importantly, FMNL2 overexpression rescued miR-613-induced suppression of proliferation, migration, and invasion of CRC cells. These results suggest that miR-613 functions as a tumor suppressor in the progression of CRC by regulating FMNL2. 

Retracted: Yes

PubPeer comments:
 A pair of flow-cytometry histograms appears in two papers by different teams of authors, representing the results of two quite different experiments. *[right]* Fig 2C.  Results of flow cytometric analysis of cell-cycle distribution of **SW480** cells after transfection with **miR-613** mimic or miR-NC . *[left]* Fig 2D from [Yu et al (2016)] .  ... cell cycle ... determined in **MMS1** cells after transfected with **miR-497** mimic or miR-NC. ![file] , 
 Fig 6B.  SW480 cells were transfected with FMNL2 expression plasmid (pcDNA3.1-FMNL2) and high levels of miR-613, and FMNL2 ... protein (B) were determined by ... western blot . ![file] Similarities among the blobs are more evident at increased contrast (right). , 
 Retracted [15 January 2022] .  Falsified materials and or data reported in this publication had been detected by a knowledgeable reader and subsequently confirmed by further investigation of the editorial office. Therefore, the entire article has been retracted in accordance with this journalu2019s policy and editorial decision. ,