Identification and characterization of GRIM-1, a cell-death-associated gene product
Authors: Edward R Hofmann
Daniel J Lindner
Jonathan D&#x27;Cunha
Limei Lin
Shreeram C Nallar
Xiao Weihua
Dhananjaya V Kalvakolanu
Journal: Journal of Cell Science (2010)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: genome-wide; technical knockout; GRIM genes; IFN; retinoic-acid

Abstract:  Using a genome-wide technical knockout, we isolated a newly identified set of GRIM (genes associated with retinoid-interferon-induced mortality) genes; GRIM genes mediate IFN- and retinoic-acid (RA)-induced cell death. Here, we describe the isolation and characterization of one such gene, GRIM-1. Three proteins, with identical C-termini, were produced from the GRIM-1 open reading frame when this gene was transcribed and translated in vitro. These protein isoforms, designated GRIM-1alpha, GRIM-1beta and GRIM-1gamma, differentially suppressed growth via apoptosis in various cell lines. We also show that a caspase-dependent mechanism generates the proapoptotic GRIM-1 isoforms. Lastly, GRIM-1 isoforms differentially blocked maturation of 18S ribosomal RNA, consistent with their respective growth-suppressive ability. Together, these studies identified a novel protein involved in growth suppression and cell death. 

Retracted: Yes

PubPeer comments:
 In Figure 2B, bands in lane #3 (old leaves) and #5 (tubers) are much more similar than expected. Could the authors please provide the raw data at higher resolution? ![file] , 
 In Figure 4B, blue boxes: bands are much more similar than expected, just horizontally flipped. ![file] , 
 In Figure 4C, much more similar than expected: ![file] , 
 Dear Editor, I will try to find all the materials and attach them again. Each of the experiments has been repeated 3 times, so I hope that they have survived with any films after so many years. The photos were strongly brightened and sharpened, Please let me know by when I should attach the documentation and if there is any technical repetition I can find. Kind regards, Agnieszka Kieu0142bowicz-Matuk , 
 #4 Dear Dr. Kieu0142bowicz-Matuk, thank you for your reply. Looking forward to seeing the raw data. Regarding  when , the sooner the better. Not sure what you mean with  technical repetition I can find . Regards. , 
 In Figure 3C, red boxes: much more similar than expected. Could the authors please search for the raw data at higher resolution of Fig. 3C too? ![file] , 
 Figure 6B, much more similar than expected, and there could be more. ![file] , 
 A band of Figure 2B (left side) reappears in Figure 3C (right side) of a different article (Kieu0142bowicz-Matuk et al. 2017 PCE) published by the same group three years later. Astonishingly, while in Fig 2B the western blot is supposed to show the distribution of **SsBBX24** in organs of Solanum plants, the same band in Fig. 3C is supposed to highlight **CBP20** a protein marker of the nucleoplasm. ![file] , 
 #3 As shown before, Figure 4C (left side) seems to have a three-time repeated band. The same band (with a peculiar submarine shape) seems to reappear in Figure 3C (right side) of a different article (Kieu0142bowicz-Matuk_et_al_2017 PCE) published by the same group three years later. Astonishingly, while in Fig 4C the band is supposed to show **UGPase**, the same band in Fig. 3C is supposed to highlight **H3** a protein marker in the nucleus. ![file] , 
 #3 Probably one more in Figure 4C (pink boxes): ![file] ,