Effects of mTOR/NF-κB signaling pathway and high thoracic epidural anesthesia on myocardial ischemia-reperfusion injury via autophagy in rats
Authors: Ri-Qi Lin
Peng Wei
Feng Huang
Jian-Lin Wen
Wei-Qiang Huang
Journal: Journal of Cellular Physiology (2018)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: autophagy; high thoracic epidural anesthesia; mTOR/NF-κB signaling pathway; myocardial ischemia reperfusion injury.
Affiliations: Cardio-Cerebrovascular Disease Precision Medical Key Laboratory Cultivation Base of Guangxi, Department of Geriatric Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China., Cardio-Cerebrovascular Disease Precision Medical Key Laboratory Cultivation Base of Guangxi, Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Abstract:  We investigated the role of mammalian target of rapamycin/nuclear factor-kappa B (mTOR/NF-κB) signaling pathway in high thoracic epidural anesthesia (HTEA) against myocardial ischemia-reperfusion (I/R) injury in rats. The rat model of myocardial I/R injury was established. Ninety rats were divided into the normal, sham, I/R, eHTEA, the PDTC, and HTEA + PDTC groups. ELISA was applied to detect cardiac function indexes. HE staining was conducted to observe histopathological changes of myocardial tissues, and TTC staining was performed to detect the myocardial infarction size. TUNEL staining was adopted to detect the cell apoptosis rate. The mRNA and protein levels of mTOR, NF-κB, Fasl, Bcl-2 and Bax, and LC3-I, LC3-II, BNIP3, and Atg5 were detected by RT-qPCR and Western blotting, respectively. The findings indicated that compared with the normal and sham groups, the I/R, PDTC, and HTEA groups showed the larger myocardial infarction size and increased cell apoptosis rate, while the results in the HTEA + PDTC group were opposite. Compared with the normal and sham groups, the I/R group showed reduced mRNA and protein levels of Bcl-2, LC3, BNIP3, and Atg5, and elevated mRNA and protein levels of mTOR, p50, p65, Bax, and Fasl, while the HTEA + PDTC group revealed the opposite results, and the PDTC and HTEA group revealed the increased mRNA and protein levels of Bcl-2, LC3, BNIP3, Atg5, mTOR, p50, p65, Bax, and Fasl. These results prove that the inhibition of mTOR/NF-κB signaling pathway potentiates HTEA against myocardial IR injury by autophagy and apoptosis in rats. 

Retracted: Yes

PubPeer comments:
 Fig 1. Panels overlap. ![file] Fig 3. Overlaps. ![file] , 
 Retracted [6 December 2021] . ------------------------------ he above article, published online on 5 December 2017 in Wiley Online Library has been retracted by agreement between the journal s Editor in Chief, Prof. Dr. Gregg Fields, and Wiley Periodicals LLC. The retraction has been agreed after the authors stated that **unintentional errors occurred** during the research process, and the experimental results cannot be verified. Thus, the conclusions are considered to be invalid. , 
 Interesting, the above images are totally different from the journal site. The retraction is wrong? Please check https: onlinelibrary.wiley.com doi 10.1002 jcp.26320 , 
 #3 Images have evidently changed from the version that passed peer-review. ![file] For instance, the panel of Fig 1 that had originally been  I R underwent a 180-deg flip and became  PDTC in the version that was retracted. ![file] ,