Identification of the porcine G protein-coupled receptor 41 and 43 genes and their expression pattern in different tissues and development stages
Authors: Zhenjin Zhou
Hao Su
Wen Yao
Genlai Li
Journal: PLoS ONE (2014)
Discipline: Multidisciplinary
Keywords: Short-chain fatty acids; SCFAs; G protein-coupled receptor; GPR41; GPR43
Affiliations: Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China., Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China; Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing, Jiangsu, China.

Abstract:  Short-chain fatty acids (SCFAs) are not only an important energy source, but they also play a regulatory role in various physiological processes in humans and rodents. Current studies, mostly in humans and rodents, have revealed that SCFAs acted as endogenous ligands for G protein-coupled receptor GPR41 and GPR43. Whether proteins similar to human GPR41 and GPR43 mediate the regulatory effects of SCFAs in swine remains unclear to date. The aims of this study were to determine whether GPR41 and GPR43 genes are expressed in porcine different tissues; and whether the expression of GPR41 and GPR43 is tissue-specific and/or time-associated. The alignment results showed that pig chromosome 6 contained GPR41 and GPR43 genes. Reverse transcription polymerase chain reaction (RT-PCR) indicated that GPR41 and GPR43 were expressed in porcine various tissues. The 2218 bp and 1908 bp nucleotide sequence representing the full-length cDNA sequence of porcine GPR41 and GPR43 was obtained from the ileum and spleen using rapid amplification of cDNA ends (RACE), which were capable of encoding 335 and 329 amino acid sequences, respectively. The structure prediction revealed that porcine GPR41 and GPR43 proteins had seven putative trans-membrane domains. The real-time PCR results indicated that GPR41 and GPR43 were expressed throughout the developmental stages in a tissue-specific and time-associated manner. GPR41 and GPR43 were most highly expressed in the ileum (P&lt;0.01) and the spleen (P&lt;0.01), respectively. Western blot results showed that porcine GPR41 and GPR43 proteins were expressed in a variety of porcine tissues, including the spleen, ileum, colon, and adipose tissue. In situ GPR41 and GPR43 immunoreactivities were observed through immunohistochemistry in the spleen, ileum, colon, and adipose tissue. In conclusion, the pig genome encoded GPR41 and GPR43 genes, and these two genes were detected in a variety of porcine tissues and expressed in tissue-specific and time-associated manner. 

Retracted: No

PubPeer comments:
 September 2020 Correction, found here: https: journals.plos.org plosone article?id=10.1371 journal.pone.0239768 It reads:  After publication of this article [1], concerns were raised that in Fig 5, the adipose tissue panels for GPR41 and GPR43 appear to be derived from the same image. The authors apologize that an error was made in generating Fig 5 such that the wrong image file was included for GPR41 adipose tissue. The original images for GPR41 and GPR43 adipose tissue are provided in S1 and S2 Files, and the authors provide here an updated version of Fig 5 including the correct data. The original images supporting other panels of Fig 5 are no longer available. An Academic Editor reviewed the updated figure and underlying data and confirmed that they support the results and conclusions reported in the published article. The authors apologize for the error in the published article. With this Correction, the authors also provide underlying data supporting Fig 2 and Fig 3 of [1], in S3 and S4 Files. Here is the original Figure 5, as reported to the journal in May 2014. I thank the authors and journal for correcting this duplication. ![file] ,