Role of Small Interfering RNA Silencing Protein Kinase C-α Gene on the Occurrence of Ultrafiltration Failure in Peritoneal Dialysis Rats
Authors: Jian Xu
Zhi-Wei Sun
Jun-Feng Wang
Jian Wang
Min Weng
Hong-Ling Yuan
Jian-Zhong Tang
Ling Lin
Journal: Journal of Cellular Biochemistry (2017)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: GLUCOSE TRANSPORT; MICROVESSEL DENSITY; PERITONEAL DIALYSIS; PKC-α siRNA; REVERSED EFFECT
Affiliations: Department of Hepatobiliary Surgery, The First People's Hospital of Yunnan Province, Kunhua Hospital Affiliated to Kunming University of Science and Technology, Kunming, 650031, P. R. China., Department of Nephrology, The First People's Hospital of Yunnan Province, Kunhua Hospital Affiliated to Kunming University of Science and Technology, Kunming, 650031, P. R. China., Department of Nutrition, The First Affiliated Hospital of Kunming Medical University, Kunming, 650032, P. R. China., Department of Geriatric Cardiology, The First Affiliated Hospital of Kunming Medical University, Kunming, 650032, P. R. China., Department of Nephrology, The First Affiliated Hospital of Kunming Medical University, Kunming, 650032, P. R. China.

Abstract:  This study aims to explore the effect of PKC-α gene silencing on the occurrence of ultrafiltration failure (UFF) in peritoneal dialysis (PD) rats. Forty-eight male SD rats were collected to establish 5/6 renal resection uremic and uremic PD rats models. Rats were assigned into control, sham operation, uremia, PD-2 W (peritoneal dialysis for 2 weeks), PD-4 W (peritoneal dialysis for 4 weeks), negative control (NC) (peritoneal dialysis for 4 weeks, and injected 0.1 mg/kg blank plasmid into abdominal cavity) and PKC-α siRNA (peritoneal dialysis for 4 weeks, and injected 0.1 mg/kg PKC-α siRNA into abdominal cavity) groups. CD34 staining was performed to determine microvessel density (MVD) for peritoneal tissues. The mRNA and protein expression of PKC-α in peritoneal tissue were detected by qRT-PCR and Western blot. Compared with the control group, MVD, the mRNA and protein expression of PKC-α were significantly increased in rats of the uremia, PD-2 W, PD-4 W, NC, and PKC-α siRNA groups. Compared with the uremia group, MVD, the mRNA and protein expression of PKC-α were increased, the changes observed in the PD-4 W and NC groups were better obvious than in the PD-2 W group. In comparison with the PD-4 W and NC groups, MVD, the mRNA and protein expression of PKC-α in rats were decreased in the PKC-α siRNA group. PKC-α gene has a high expression in uremic PD rats, and PKC-α gene silencing is able to increase UF while decrease MVD and glucose transport in peritoneal tissues thus reversing UFF in PD rats. J. Cell. Biochem. 118: 4607-4616, 2017. © 2017 Wiley Periodicals, Inc. 

Retracted: No

PubPeer comments:
 Figure 1A. * Red boxes: Two panels appear to show an overlap. ![file] , 
 Corrected [8 January 2021] .  Role of Small Interfering RNA Silencing Protein Kinase Cu2010u03b1 Gene on the Occurrence of Ultrafiltration Failure in Peritoneal Dialysis Rats Zhiu2010Wei Sun, Jian Wang, Min Weng, Jianu2010Zhong Tang, Junu2010Feng Wang, Jian Xu, Ling Lin, Hongu2010Ling Yuan Journal of Cellular Biochemistry 2017 Dec;118(12):4607u20104616. The authors realized that there was an error in the results of the immunohistochemistry in Figure 1, in the NC group. The correct Figure 1 with the modified NC group is shown below. The authors regret this error. , 
 Fig 4B,  Band patterns of PKC-u03b1 expression in peritoneal tissues of rats in each group . ![] Could the authors comment on the appearance of what appears to be the same loading control in Fig 9c of [Zhang et al (2019)] ? ![] More sightings. At left, Fig 4B from [Tian et al (2018)] .  Western blot analysis demonstrates that miRu2010485 reduces the protein expressions of RhoA, ILu20106, TGFu2010u03b2, and p38MAPKs as well as the extent of pu2010p38MAPK . At right, Fig 7C from [Han et al (2018)] .  Gray value of cAMP, PKA and CREB1 protein bands . ![file] ,