Retracted : Effects of long noncoding RNA SPRY4‐IT1‐mediated EZH2 on the invasion and migration of lung adenocarcinoma
Authors: Yong-Jian Wang
Dong-Mei Wu
Xin-Rui Han
Bin Hu
Chun-Hui Sun
Juan Zhuang
Xin Wen
Meng-Qiu Li
Qiao Wu
Jun Lu
Shao-Hua Fan
Qun Shan
Jun-Hua Tan
Zi-Feng Zhang
Yuan-Lin Zheng
Journal: Journal of Cellular Biochemistry (2018)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: SPRY4-IT1; enhancer of zeste homolog 2; invasion; long noncoding RNA; lung adenocarcinoma
Affiliations: Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu ProvinceSchool of Life ScienceJiangsu Normal UniversityXuzhouP. R. China, School of Environment Science and Spatial InformaticsChina University of Mining and TechnologyXuzhouP. R. China, Jiangsu Key Laboratory for Eco‐Agricultural Biotechnology around Hongze LakeSchool of Life SciencesHuaiyin Normal UniversityHuaianP. R. China, Department of OncologyCangzhou Central HospitalCangzhouP. R. China

Abstract:  We aim to investigate the interaction between the EZH2 and the long noncoding RNA (lncRNA) SPRY4-IT1. We also explore their respective effects on human lung adenocarcinoma (LA) cell invasion and migration. Both LA and adjacent normal tissues were obtained from 256 LA patients. SPTY4-IT expression and EZH2 mRNA expressions in tissues and cells were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR). The siRNAs against SPRY4-IT1 and EZH2 were co-transfected into A549 and H1975 cells. The interaction between SPRY4-IT1 and EZH2 was determined using a RNA pull-down assay and a RNA immunoprecipitation (RIP) assay. A Transwell assay and scratch assay were used to evaluate the cell migration and invasion abilities. The expressions of E-cadherin and Vimentin in the epithelial-mesenchymal transition (EMT) and EZH2 protein expression were detected through western blotting. SPRY4-IT1 expression was observed to be significantly lower, while the expression of EZH2 was higher in the LA tissues than in the adjacent normal tissues. Compared with the HBE cell line, expressions of SPRY4-IT1 in each human LA cell line had decreased, with the lowest observed reduction in the A549 cell line, while EZH2 mRNA and protein expression increased in each human LA cell lines. After SPRY4-IT1-siRNA was transfected into A549 and H1975 cells, invasion and migration abilities were enhanced, in addition to a reduction in the expression of E-cadherin, while expressions of Vimentin exhibited an increased rate. Consequently, we find that EZH2 promotes LA cell invasion and metastasis by inhibiting SPRY4-IT1 expression. 

Retracted: Yes

PubPeer comments:
 *[Right]* Figure 6A,C  Expressions of EMT-related proteins (E-cadherin and Vimentin) detected by western blotting Note: A and C, expressions of EMT-related proteins (E-cadherin and Vimentin) in A549 and H1975 cells co-transfected with si-SPRY4-IT1 and siEZH2 . *[Left]* FIGURE 5B from [Wang et al (2018)] .  Upregulation of miR-382 negatively regulates FoxO1 and increases ECM accumulation in the glomerular mesangial cells.... Panels B and C, western blot analysis was used to determine the protein expressions of FoxO1, p-FoxO1, FN, Col I, Col IV and PAI-1; the protein expression of FoxO1 does not differ among the six groups . A loading control seems to be used in both. ![file] , 
 Fig 2C,  SPRY4-IT1 and EZH2 expressions in five cell lines detected by RT-qPCR and western blotting ... C, the EZH2 protein bands in five LA cell lines detected by western blotting . ![file] Particular blobs from the loading control are familiar from *[left]* Fig. 3A from [Shen et al (2017)] ; *[right]* Fig 4B from [Wang et al (2017)] . ![file] , 
 #1 Fig 6A C again. ![] Another sighting of the control band: Fig 5B from [Wu et al (2018)] .  Expression of Wnt u03b2-catenin signaling pathway-related factors in rats of each group. ... B, relative protein expression of u03b2-catenin, GSK-3u03b2, GSK-3u03b2-Ser9 and cyclinD1 detected by western blot . ![] , 
 One more loading control. At left here, Fig 4B:  The interaction between SPRY4-IT1 expression and EZH2 expression detected by RT-qPCR and western blotting ... B and E, the SPRY4-IT1 protein bands in the eluant of RNA pull-down assay in A549 ... detected by western blotting; Positive control was the RNA provided by kit that could specially combine with HuR protein, and negative control was poly(A)25 RNA without any combination; anti-sense RNA was designed for SPRY4-IT1, which could compete with EZH2 to combine with SPRY4-IT1 . At right: Fig 6E from [Zhang et al (2018)] .  Verification of the relations among miR-300, CUL4B, Wnt u03b2-catenin pathway and EMT ... E, protein bands of GAPDH, CUL4B, Wnt, u03b2-catenin, N-cadherin and Snail in Capan-1 cell line . Loading control bands are more similar than I would have expected. ![file] Throwing Fig 5B into the mix as well *[now at left]* :  Expressions of miR-708, bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI), Wnt10B, P53, Bcl-2, VEGF, Fas, Bax, caspase-3, and cleaved caspase-3 in each group. A, Histogram of mRNA expressions. B, Electrophoretograms of protein expressions. ![file] , 
 Fig 9C.  Effects of siR-SPRY4-IT1 expression on the invasion and metastasis abilities of A549 and H1975 cells ... C, the Transwell assay detection of the invasion ability of H1975 cells in the four groups evaluated by Transwell assay . ![file] Panels appear, differently labeled, in Fig 9(a,c) of [Han et al (2019)] .  FOSL1 silencing exerts a negative effect on OS cell invasion (u00d7200). (a,b) Transwell assay results suggested that in Saos2 cell line, OS cell invasion was inhibited by FOSL1 depletion. (c,d) Transwell assay exhibited that in MG63 cell line, OS invasion was inhibited by FOSL1 depletion . ![file] , 
 Retracted [30 June 2021] . -------------------------------------------- The above article, published online on 10 August 2017 in Wiley Online Library has been retracted by agreement between the the journal s Editor in Chief, Prof. Dr. Christian Behl, and Wiley Periodicals LLC. The retraction has been agreed following an investigation based on allegations raised by a third party. A detailed investigation revealed that several image elements of the experimental data were published elsewhere in a different scientific context. Thus, the editors consider the conclusions of this article to be invalid. , 
 Assessed here, as part of the first of 10 broad investigations: https: www.nsfc.gov.cn publish portal0 tab442 info85495.htm **Decisions on handling scientific research misconduct cases investigated and dealt with in 2022 (first batch)** Date 2022-05-13 Source: u3000 Author: u3000 u3010 Large, Middle and Small u3011 u3010Printu3011 u3010Closeu3011 u3000u3000The National Natural Science Foundation of China resolutely implements the spirit of the  Several Opinions on Further Strengthening the Construction of Scientific Research Integrity and  Opinions on Further Promoting the Spirit of Scientists and Strengthening the Construction of Style and Study Style by the General Office of the Central Committee of the Communist Party of China and the General Office of the State Council. Horizontal science and technology are self-reliant and self-reliant, continue to carry out scientific research integrity construction, and seriously investigate and deal with scientific research misconduct involving the National Natural Science Foundation of China. The Supervision Committee of the National Natural Science Foundation of China conducts investigations, verifications and comprehensive judgments on the accepted scientific research integrity cases, in accordance with the Regulations of the National Natural Science Foundation of ) and  Measures for Investigation and Handling of Scientific Research Misconduct in National Natural Science Foundation of China and other regulations and systems to propose handling suggestions. After deliberation at the second executive meeting of the National Natural Science Foundation of China in 2022, it was decided to deal with the relevant parties involved. The 10 handling decisions (online release version) that have been criticized and criticized are hereby announced. -------------------------------- About the existence of papers published on Wu Dongmei, Zheng Yuanlin, Lu Jun, etc. - Falsification of pictures or data, fabrication of research process, use of others signatures without consent, and false information in project final reports - processing decision - National Science and Technology Financial Supervisory Office [2022] No. 1 u3000u3000The Supervision Committee of the National Natural Science Foundation of China launched an investigation into several papers of Jiangsu Normal University, including Wu Dongmei, Zheng Yuanlin, and Jun Jun, for suspected academic misconduct. The papers involved are as follows: ... Paper 34:  Xin Wen # , Xin-Rui Han # , Yong-Jian Wang # , Shao-Hua Fan, Juan Zhuang, Zi-Feng Zhang, Qun Shan, Meng-Qiu Li, Bin Hu, Chun-Hui Sun, Qiao Wu, Jun-Hua Tan, Dong-Mei Wu*, Jun Lu*, Yuan-Lin Zheng*. **Effects of long noncoding RNA SPRY4-IT1-mediated EZH2 on the invasion and migration of lung adenocarcinoma**. Journal of Cellular Biochemistry, 2018, 119(2):1827-1840.u201d (marked with fund numbers 81271225, 31201039, 81171012, 30950031, 81571055, 81400902) ... u3000u3000According to the investigation of the supporting unit, the above 35 papers involved all have the problem of improper use of pictures or data fraud, and the pictures or data in question are all provided by third-party companies. Among the 35 papers involved, 34 (except paper 21) still have the problem of using others signatures without consent, and papers 4, 5, 9, 12, 30, and 34 also have the problem of fabricating the research process. Wu Dongmei is the author of the above 35 papers involved, of which 12 papers (papers 2, 8, 9, 10, 11, 12, 14, 15, 25, 26, 30, 35) are the first author and 22 papers (Paper 1, 4, 5, 6, 7, 13, 16, 17, 18, 19, 20, 21, 22, 23, 24, 27, 28, 29, 31, 32, 33, 34) Corresponding author, They should be primarily responsible for the objective results of falsifying pictures or data, using others signatures without consent, and fabricating research processes; Zheng Yuanlin and Lu Jun, as the co-corresponding authors of the 35 papers involved, were negligent in reviewing and took secondary responsibility. In addition, Wu Dongmei included 4 papers involved (papers 21, 24, 28, 29) in the final report of the National Natural Science Foundation of China (Grant No. 81400902), and Zheng Yuanlin included 9 papers involved (papers 6, 8, 10, 13, 21, 24, 26, 28, 29) included in the final report of the National Natural Science Foundation of China (Grant No. 81571055), and should be responsible for the objective results of false information in the final report. u3000u3000After deliberation at the 12th meeting of the Fifth Supervision Committee of the National Natural Science Foundation of China (Life Medicine Professional Committee) and the approval of the second executive meeting of the National Natural Science Foundation of China in 2022, it was decided to follow the thirty-fifth National Natural Science Foundation of China Regulations. Article 4, Article 17, Items 3, 4, and 9 of  Measures for Handling Misconduct in Science Fund Work by the Supervision Committee of the National Natural Science Foundation of China (for Trial Implementation) , revocation of Wu Dongmei s National Natural Science Foundation of Molecular mechanism of TRPV4-mediated domoic acid treatment-induced cognitive deficits in mice (Grant No. 81400902), recovering the allocated funds, permanently disqualifying Wu Dongmei from the National Natural Science Foundation of China (since January 18, 2022), Give Wu Dongmei a circular of criticism; decide to follow Article 35, item 4 of the  National Natural Science Foundation of China Regulations , and refer to  The National Natural Science Foundation of China Supervision Committee s Handling of Misconduct in Science Fund Work (Trial) Tenth Items 3 and 4 of seven articles, the Zheng Yuanlin National Natural Science Foundation of China project  The Mechanism of HDAC6 Activating HMGB1 RAGE Signaling Axis to Mediate Cognitive Defects in Type 2 Diabetic Mice (Approval No. 81571055), recover the allocated funds, cancel Zheng Yuanlin will be eligible to apply for the National Natural Science Foundation of China for 5 years (January 18, 2022 to January 17, 2027), and will give Zheng Yuanlin a notice of criticism; it is decided to refer to the  National Natural Science Foundation of China Supervision Committee on misconduct in the work of the National Natural Science Foundation of China. Items 3 and 4 of Article 17 of the Measures (for Trial Implementation), the Army s National Natural Science Foundation of China project application qualification will be cancelled for 5 years (January 18, 2022 to January 17, 2027), and the Army will be criticized in a circular. - National Natural Science Foundation of China - March 30, 2022 ,