Downregulation of NEAT1 reverses the radioactive iodine resistance of papillary thyroid carcinoma cell via miR-101-3p/FN1/PI3K-AKT signaling pathway
Authors: Jialun Zhu
Ting Chen
Juan Lv
Pengjie Liu
Fukun Chen
Chuanzhou Yang
Chao Liu
Li Jia
Zhiyong Deng
Zhiping Feng
Journal: Cell Cycle (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: FN1; NEAT1; PI3K/AKT signaling pathway; PTC; RAI-resistance
Affiliations: a Department of Nuclear Medicine , Yunnan Cancer Hospital & The Third Affiliated Hospital of Kunming Medical University & Yunnan Cancer Center , Kunming , Yunnan , China.

Abstract:  Considering the resistance of papillary thyroid cancer (PTC) 131I therapy, this study was designed to find a solution at molecular respect. By probing into lncRNA-NEAT1/miR-101-3p/FN1 axis and PI3K/AKT signaling pathway, this study provided a potential target for PTC therapy. 131I-resistant cell lines were established by continuous treatment with median-lethal 131I. Bioinformatic analysis was applied to filtrate possible lncRNA/miRNA/mRNA and related signaling pathway. Luciferase reporter assay was employed in the verification of the targeting relationship between lncRNA and miRNA as well as miRNA and mRNA. MTT assay and flow cytometry assay were performed to observe the impact of NEAT1/miR-101-3p/FN1 on cell viability and apoptosis in radioactivity iodine (RAI)-resistant PTC cell lines, respectively. Western blot and qRT-PCR were conducted to measure the expression of proteins and mRNAs in RAI-resistant PTC tissues and cells. Meanwhile, endogenous PTC mice model were constructed, in order to verify the relation between NEAT1 and RAI-resistance in vivo. NEAT1 was over-expressed in RAI-resistant PTC tissues and cell lines and could resist RAI by accelerating proliferation accompanied by suppressing apoptosis. It indicated that overexpressed NEAT1 restrained the damage of RAI to tumor in both macroscopic and microcosmic. Besides, NEAT1/miR-101-3p exhibited a negative correlation by directly targeting each other. The expression of FN1, an overexpressed downstream protein in RAI-resistance PTC tissues, could be tuned down by miR-101-3p, while the decrease could be restored by NEAT1. In conclusion, both in vitro and in vivo, NEAT1 suppression could inhibit 131I resistance of PTC by upregulating miR-101-3p/FN1 expression and inactivated PI3K/AKT signaling pathway both in vitro and in vivo. 

Retracted: Yes

PubPeer comments:
 Two flow cytometry dot plots have been duplicated and presented as something else: Figure 5e: first plot and third plot from left. Figure 6e: second plot vs last plot in Figure 5e. The Western blots appear suspicious (artificial look, no background). ![file] , 
 Still waiting for a response from the papermill who produced this paper. Zhiyong Deng s e-mail address is dorwangjq@163.com... , 
 Google indicates that the corresponding email address was previously used in 2012, attached to a researcher in Qingdao. ![file] , 
 Retracted [15 July 2022] . -------------------------------- We, the Editors and Publisher of the journal Cell Cycle, have retracted the following article: Chao Liu, Zhiping Feng, Ting Chen, Juan Lv, Pengjie Liu, Li Jia, Jialun Zhu, Fukun Chen, Chuanzhou Yang, and Zhiyong Deng. Downregulation of NEAT1 reverses the radioactive iodine resistance of papillary thyroid carcinoma cell via miR-101-3p FN1 PI3K-AKT signaling pathway. Cell Cycle. 2018;18(2):167u2013203. doi: 10.1080 15384101.2018.1560203. Since publication, concerns have been raised about the integrity of the data in the article. When approached for an explanation, the authors checked their data and confirmed there are fundamental errors present. Therefore, they have agreed to the retraction of this article. The authors apologise for this oversight. We have been informed in our decision-making by our policy on publishing ethics and integrity and the COPE guidelines on retractions. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as u2018Retractedu2019. ,