Down-regulation of LINC00472 promotes osteosarcoma tumorigenesis by reducing FOXO1 expressions via miR-300
Authors: Bizeng Zhao
Weifeng Ni
Jingwei Zhang
Haijun Xiao
Jieyuan Zhang
Dong Zhang
Journal: Cancer Cell International (2020)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: FOXO1; LINC00472; Osteosarcoma; miR-300.
Affiliations: Department of Orthopedics, Shanghai Fengxian District Central Hospital/Southern Medical University Affiliated Fengxian Hospital, No. 6600 Nanfeng Road, Shanghai, 201499 China., 2Department of Orthopedics, Shanghai Sixth People's Hospital, No. 600 Yishan Road, Shanghai, 200233 China.

Abstract:  Background: Osteosarcoma (OS) is one of the most common types of primary bone tumors which poses negative effects on the bones of both young children and adolescents. LncRNA LINC00472 has been reported to be involved with poor prognostics in breast cancer and ovarian cancer. As a new lncRNA, its role in OS remains to be elusive. Herein, we are focused to explore its regulatory mechanism in the development of OS. Methods: qRT-PCR was utilized to examine the expressions of LINC00472 and miR-300 in OS tissues and cell lines. OS cell lines of U2OS and MG63 were used to investigate the biological function of LINC00472. Xenograft tumor model was built in nude mice with MG63 cells. Results: The expressions of LINC00472 were inhibited in OS tissues and cells, and were negatively related to the expressions of miR-300. LINC00472 directly targeted miR-300. FOXO1 was inhibited in OS tissues and its expressions were negatively related to the expressions of miR-300. LINC00472 over-expressions decreased cell proliferation abilities and colony formation abilities. These effects were mediated by miR-300. The silence of LINC00472 and over-expressions of miR-300 suppressed FOXO1 expressions. LINC00472 greatly reduced tumor growth in vivo and this effect was attenuated by miR-300 mimic. Conclusions: From all the experiments and observations, we demonstrated that LINC00472 could be a potential tumor suppressor in OS through interacting with miR-300 and FOXO1. 

Retracted: No

PubPeer comments:
 An image in Figure 3b seems unexpectedly similar to Figure 4C in a paper submitted a month apart, Molecular Medicine (2020), doi: 10.1186 s10020-020-00200-3, discussed here: https: pubpeer.com publications 289742E023EAB5FBBFCC8EB38866A3 There does not appear to be any overlap between the authors or affiliated institutions. Even if this was an error during figure assembly, could the authors explain how both groups had access to this image? It suggests that perhaps a common group prepared both papers. *** ![file] , 
 A different image in Figure 3b seems unexpectedly similar to an image in Figure 4C of a different paper, Brazilian Journal of Medical and Biological Research (2021), doi: 10.1590 1414-431x202010222, discussed here: https: pubpeer.com publications 51FACD700BCEB2DE71D4748E96BE72 Interestingly, the light reflections that appear in this paper are absent in the Brazilian Journal paper, one area noted by red arrows. TAM. *** ![file] , 
 #1 ![] Another sighting! Fig 3F of  microRNA-18a from M2 Macrophages Inhibits TGFBR3 to Promote Nasopharyngeal Carcinoma Progression and Tumor Growth via TGF-u03b2 Signaling Pathway ([Peng et al 2020] ). ![] , 
 - *[above]* Fig 5c. - *[below]* Fig 5A from  Exosomal miRNA-205 promotes breast cancer chemoresistance and tumorigenesis through E2F1 ([Zhao et al 2021] ). ![file] ,