Knockdown of long noncoding RNA linc-ITGB1 inhibits tumor metastasis in colorectal cancer through suppressing BDNF
Authors: W-B Wan
Q-L Kong
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: lncRNA; linc-ITGB1; colorectal cancer; RT-qPCR; metastasis
Affiliations: Department of Gastrointestinal Surgery, Heze Municipal Hospital, Heze, China. .

Abstract:  Objective: Recently, long noncoding RNA (lncRNAs) have got much attention for their role in the progression of cancers. In this research, lncRNA linc-ITGB1 was studied to identify whether it affects the development of colorectal cancer (CRC). Patients and methods: Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was performed to detect the linc-ITGB1 expression of CRC cells and tissues. Moreover, the associations between linc-ITGB1 expression level and patients' overall survival rate were further analyzed. Furthermore, we conducted function assays, including wound healing assay and transwell assay, to explore the effect of linc-ITGB1 on CRC metastasis in vitro. In addition, the underlying mechanism was further studied. Results: RT-qPCR results showed that linc-ITGB1 expression level was higher in CRC samples than that in adjacent tissues. Linc-ITGB1 expression was related closely to patients' overall survival time. Moreover, cell migration and cell invasion were inhibited after linc-ITGB1 was silenced in vitro. In addition, the mRNA and protein expression of BDNF was downregulated by the silence of linc-ITGB1. Furthermore, the expression level of BDNF was higher in CRC samples than that in adjacent tissues and was positively related to the expression of linc-ITGB. Conclusions: These results indicate that linc-ITGB1 could enhance CRC cell migration and invasion via upregulating BDNF. Linc-ITGB1 might be a potential therapeutic target for CRC patients. 

Retracted: Yes

PubPeer comments:
 This paper is one of a set of 121 papers that all contain images from the same library of about 100 photos and plots. This is unexpected, because each paper has different authors and different affiliations, with little to no overlap, and describes different cancer types and molecules. It would be very hard to explain how authors from different labs would all obtain the same photo. Therefore, the papers might have all be derived from the same source, such as a paper mill that sells ready-to-be-published papers. This  Stock Photo paper set has been described in more detail on my blog post, https: scienceintegritydigest.com 2020 07 05 the-stock-photo-paper-mill and in a Wall Street Journal article by Eva Xiao (currently behind a paywall): https: www.wsj.com articles chinese-research-papers-raise-doubts-fueling-global-questions-about-scientific-integrity-11593939600 The vast majority (113 121) of the papers were published in the same journal, European Review for Medical and Pharmacological Sciences (ERMPS). A link to the full set of papers can be found here: https: docs.google.com spreadsheets d 172RWS18w1lTNJ9Nnj80fuXXTqTm4fGkoK0sdYyvKE3g edit?usp=sharing All papers have been reported to the journal editors on July 2, 2020. , 
 Retracted: https: www.europeanreview.org article 22195  Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cKnockdown of long noncoding RNA linc-ITGB1 inhibits tumor metastasis in colorectal cancer through suppressing BDNF, by W.-B. Wan, Q.-L. Kong, published in Eur Rev Med Pharmacol Sci 2019; 23 (15): 6453-6458u2013DOI: 10.26355 eurrev_201908_18528u2013PMID: 31378884u201d has been withdrawn. The Publisher apologizes for any inconvenience this may cause. , 
 The [Report from the Affiliated Hospital of Jining Medical College] reads as follows (thanks, Google Translate): --------------------------- 4. The issue of u201cKnockdown of long noncoding RNA linc-ITGB1 inhibits tumor metastasis in colorectal cancer through suppressing BDNFu201d in which Kong Qingli from the Affiliated Hospital of Jining Medical College is the corresponding author. After investigation, the paper  Knockdown of long noncoding RNA linc-ITGB1 inhibits tumor metastasis in colorectal cancer through suppressing BDNF in which Kong Qingli of the Affiliated Hospital of Jining Medical College is the corresponding author has the problem of ghostwriting and investment. The Affiliated Hospital of Jining Medical College will deal with the relevant responsible persons as follows: The corresponding author Kong Qingli will be (1) ordered to make an in-depth inspection; (2) ordered to withdraw the manuscript of the papers involved; (3) cancel the five-year application for scientific and technological plan projects (special projects, funds, etc.), scientific and technological awards, and titles of scientific and technological talents, etc. Eligibility, from July 13, 2020 to July 12, 2025; (4) Cancellation of five-year postgraduate tutor application and appointment qualifications from July 13, 2020 to July 12, 2025 ; (5) Cancellation of three-year professional title promotion qualifications from July 13, 2020 to July 12, 2023; (6) Criticism is reported in the hospital. ,