microRNA-206 overexpression inhibits cellular proliferation and invasion of estrogen receptor α-positive ovarian cancer cells
Authors: Yan Li
Zhengfang Wen
Shaoru Li
Wenhui Liu
Xinlei Guan
Fanjing Kong
Journal: Molecular Medicine Reports (2014)
Discipline: CLINICAL MEDICINE
Keywords: estrogen receptor; ERα; microRNA; miR-206; tumor suppressor
Affiliations: Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China., Department of Endocrinology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China., School of Public Health, Shandong University, Jinan, Shandong 250100, P.R. China.

Abstract:  The expression levels of estrogen receptor (ER α) are closely associated with estrogen-dependent growth, invasion and response to endocrine therapy in ERα-positive ovarian cancer. However, the underlying regulatory mechanisms remain to be fully understood. Previous studies have demonstrated that ERα is a direct target of microRNA (miR)-206. miR-206 has been found to be an important tumor suppressor in several cancer types, including ovarian, gastric and laryngeal cancer. However, the specific role of miR-206 in ovarian cancer remains unclear. The aim of the present study was to investigate the role of miR-206 in ER-a positive ovarian cancer in vitro. The present study demonstrated that miR-206 is significantly downregulated in ERα-positive but not ERα‑negative ovarian cancer tissues, compared with normal ovarian epithelium tissue. It was also found that the expression of miR-206 was decreased in ERα-positive ovarian cancer cell lines, CAOV-3 and BG-1, compared with normal ovarian epithelium tissues. This suggests that miR-206 may play a role in ERα-positive ovarian cancer cells via an estrogen-dependent mechanism. Further analysis revealed that 17β-E2 treatment significantly promoted cellular proliferation and invasion of estrogen-dependent CAOV-3 and BG-1 cells, which could be reversed by the introduction of miR-206 mimics. In conclusion, the present study suggests that miR-206 has an inhibitory role in estrogen-dependent ovarian cancer cells. Thus, miR-206 may be a promising candidate for the endocrine therapy of ERα-positive ovarian cancer. 

Retracted: Yes

PubPeer comments:
 Fig 1A.  Western blotting was performed to examine the protein expression of ERu03b1 in 15 ovarian cancer samples. GADPH was used as an internal reference. The relative protein level of ERu03b1 was determined by the grey value of ERu03b1 GADPH. Samples with grey values of ERu03b1 GADPH 0.1 were grouped into the ERu03b1-positive group, while the rest were grouped into the ERu03b1-negative group . ![file] For comparison: Figs 2B and 1B of [Peng et al (2013)] . ![file] , 
 Fig 4A.  Transwell assay was performed to determine the invasion capacity in each group following incubation for 24 h at 37u00b0C and 5% CO2 . ![file] Compare with Fig 4 A,B of [Wen et al (2015)] .  miR-148a inhibits ovarian cancer cell migration and invasion. (A) A cell migration assay was performed to determine the migration capacity of SKOV3 cells transfected with miR-148a mimics, S1PR1 siRNA or co-transfected with miR-148a mimics and S1PR1 plasmid ... (B) A cell invasion assay was performed to determine the cellular invasion capacity of SKOV3 cells transfected with miR-148a mimics, S1PR1 siRNA or co-transfected with miR-148a mimics and S1PR1 plasmid . ![file] Fig 7 from [Wang et al (2014)] .  Cell invasion assay was performed to determine the effect of miR-195 and CARMA3 on invasion in SW480 and HT29 cells following transfection with an miR-195 mimic or an miR-195 inhibitor, or co-transfected with an miR-195 mimic and CARMA3-pcDNA3.1 . ![file] , 
 #2 Fig 4A again, this time color-coding different panels for consistency with a different repertoire of invasion-assay images. ![file] Compare with Figs 2E, 6E from [Ouyang, Yuan Qiu (2018)] (rotated through 90 deg). ![file] Figs 2D, 5E from [Liang et al (2017)] ... ![file] ...Figs 3 C,D and 8 C,D from [Liu et al (2017)] . ![file] Other manifestations are linked in those threads. , 
 Retracted [9 July 2021] . ----------------------------------- Following the publication of this paper, it was drawn to the Editors attention by a concerned reader that certain of the western blotting data shown in Fig. 1A and Transwell cell migration data shown in Fig. 4A were strikingly similar to data appearing in different form in other articles by different authors. Owing to the fact that the contentious data in the above article had already been published elsewhere, or were already under consideration for publication, prior to its submission to *Molecular Medicine Reports*, the Editor has decided that this paper should be retracted from the Journal. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive any reply. The Editor apologizes to the readership for any inconvenience caused. ,