Long noncoding RNA MIAT promotes the growth and metastasis of non-small cell lung cancer by upregulating TDP43
Authors: Y-B Zhao
S-Q Xu
X Li
M-P Yang
Q Li
H-L Zhao
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: lncRNA; MIAT; non-small cell lung cancer; NSCLC; RT-qPCR
Affiliations: Department of Medicine, Harbin Medical University Cancer Hospital, Harbin, China. .

Abstract:  Objective: Recent researches have proved that long noncoding RNAs (lncRNAs) act and have an important role in many diseases. In this research, lncRNA MIAT was explored to identify how it functions in the development of non-small cell lung cancer (NSCLC). Patients and methods: Real-time quantitative polymerase chain reaction (RT-qPCR) was utilized to detect MIAT expression in NSCLC patients. Next, we conducted cell counting kit-8 (CCK-8) assay, colony formation assay, ethynyl deoxyuridine (EdU) incorporation assay, wound healing assay and transwell assay to identify its biological function. Further experiments were performed to explore the potential mechanism. Results: By comparing with MIAT expression in adjacent tissues, MIAT expression level was significantly higher in NSCLC samples. Moreover, functional assays showed that cell growth ability of NSCLC cells was inhibited after MIAT was knocked down. In addition, the migrated and invaded ability of NSCLC cells was inhibited after MIAT was knocked down. Furthermore, the expression of TDP43 was downregulated by knockdown of MIAT. Meanwhile, it was found that TDP43 expression positively correlated to MIAT expression in NSCLC tissues. Conclusions: Results above suggest that MIAT could enhance cell proliferation and metastasis of NSCLC by upregulating TDP43, which suggests that MIAT may be a potential therapeutic target in NSCLC. 

Retracted: Yes

PubPeer comments:
 This paper is one of a set of 121 papers that all contain images from the same library of about 100 photos and plots. This is unexpected, because each paper has different authors and different affiliations, with little to no overlap, and describes different cancer types and molecules. It would be very hard to explain how authors from different labs would all obtain the same photo. Therefore, the papers might have all be derived from the same source, such as a paper mill that sells ready-to-be-published papers. This  Stock Photo paper set has been described in more detail on my blog post, https: scienceintegritydigest.com 2020 07 05 the-stock-photo-paper-mill and in a Wall Street Journal article by Eva Xiao (currently behind a paywall): https: www.wsj.com articles chinese-research-papers-raise-doubts-fueling-global-questions-about-scientific-integrity-11593939600 The vast majority (113 121) of the papers were published in the same journal, European Review for Medical and Pharmacological Sciences (ERMPS). A link to the full set of papers can be found here: https: docs.google.com spreadsheets d 172RWS18w1lTNJ9Nnj80fuXXTqTm4fGkoK0sdYyvKE3g edit?usp=sharing All papers have been reported to the journal editors on July 2, 2020. , 
 #1 Dear editor. Thank you for your comments for our manuscript. Actually, we sent our manuscript to a company for language polishing and modification before submitting. Now, we have noticed the figures had some differences with our manuscript version. We are still surveying what happened to our paper. Based on the results, we decided to withdraw our paper from the journal. Thank you for your concern and we are really sorry for bothering you. Wish you all the best in such an epidemic situation. , 
 #2 We are really sorry. , 
 The publishers have wished the paper into the cornfield: https: www.europeanreview.org article 21860 Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cLong noncoding RNA MIAT promotes the growth and metastasis of non-small cell lung cancer by upregulating TDP43, by H.-L. Zhao, S.-Q. Xu, Q. Li, Y.-B. Zhao, X. Li, M.-P. Yang, published in Eur Rev Med Pharmacol Sci 2019; 23 (8): 3383-3389u2013DOI: 10.26355 eurrev_201904_17702u2013PMID: 31081093u201d has been withdrawn. The Publisher apologizes for any inconvenience this may cause. The PDF has disappeared from their site, but if anyone is interested, a copy was saved on the Wayback Machine here: https: web.archive.org web 20200215013207 https: www.europeanreview.org wp wp-content uploads 3383-3389.pdf , 
 http: www.most.gov.cn zxgz kycxjs kycxgzdt 202201 t20220127_179206.html Google Translated Date: January 27, 2022 14:44 Source: Ministry of Science and Technology u3000u300027. The paper  Long noncoding RNA MIAT promotes the growth and metastasis of non-small cell lung cancer by upregulating TDP43 with Zhao Hongli as the first author and Yang Maopeng as the corresponding author, Harbin Medical University Cancer Hospital. After investigation, it was found that the paper was written, voted, and improperly signed. Zhao Hongli will be dealt with as follows: order to withdraw the manuscript, exhort her to talk about scientific research integrity, cancel her application for science and technology plans (special projects, funds, etc.) projects and award qualifications for 5 years, cancel her qualifications for promotion to postgraduate tutors for 4 years, and cancel her qualifications for promotion to professional titles for 3 years; Yang Maopeng will be dealt with as follows: the scientific research integrity admonishment talk, the research award of the paper will be withdrawn, and all the bonus will be withdrawn, his application for science and technology plan (special projects, funds, etc.) projects and award qualifications will be cancelled for 3 years, and the qualification for academic degree postgraduate enrollment will be cancelled for 3 years. Self-examination of the research of other members of the research group within 1 year to avoid similar incidents. u3000u3000(Notification link: https: www.hrbmu.edu.cn kyc info 1026 1320.htm) ,