Effects of exercise training on mobilization and functional activity of blood-derived progenitor cells in patients with acute myocardial infarction
Authors: Michael Brehm
Ch Schannwell
M Köstering
P Schüller
E Bölke
F Picard
P Ebner
K Augusta
D Ilousis
G Turan
M Peiper
B E Strauer
T Fleissner
Journal: European journal of medical research (2009)
Discipline: CLINICAL MEDICINE
Keywords: exercise training; CPCs; myocardial infarction; STEMI; BNP levels
Affiliations: Department of Internal Medicine, Division of Cardiology, Pneumology and Angiology, Heinrich-Heine-University, Moorenstr. 5, 40 225 Düsseldorf, Germany. 

Abstract:  Background: The aim of the present study was to determine whether regular exercise training (ET) is effective at promoting the mobilization of CPCs and improving their functional activity in patients with recently acquired myocardial infarction (STEMI). Regular physical training has been shown to improve myocardial perfusion and cardiovascular function. This may be related in part to a mobilization of bone marrow-derived circulating progenitor cells (CPCs) as well as an enhanced vascularisation. Methods: 37 patients with STEMI were randomly assigned to an ET group or a non-ET group (controls). Two weeks after STEMI, three weeks after regular ET and three months after ET, BNP levels, exercise echocardiography and exercise spiroergometry were evaluated. The number of CD34+/CD45+ and CD133+/CD45+ CPCs was measured by flow cytometry analysis. The migration capacity of the CPCs was determined with a boyden chamber and the clonogenic capacity by CFU-assay. Results: In the ET-group the number and migration capacity of CPCs increased significantly after regular exercise training. The BNP level decreased significantly from 121 +/- 94 to 75 +/- 47 pg/ml (p&lt;0.001) after the ET period, the left ventricular ejection fraction raised in parallel at peak exercise, and the cardiorespiratory condition improved as demonstrated by an increase of VO2max (from 1641 +/- 522 to 1842 +/- 724 ml/min, p&lt;0.02). These three effects persist till three months after the ET period. Conclusions: Regular physical activity appears to predispose the mobilization and enhanced functional activity of CPCs, a phenomenon which might lead to an improved cardiac function in patients with recently acquired acute myocardial infarction. 

Retracted: No

PubPeer comments:
 unexpected image duplication ![file] Disclaimer: These potential image duplications have been detected automatically using ImageTwin http: dx.doi.org 10.1145 3019612.3019875 Although results have been curated, the automatic algorithm may have generated false positive results and cannot take into account all scientific explanations for apparent duplications like stripped and reprobed blots, time series, serial sections and reused controls. , 
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 According to the legend fig. 4. shows photos of a migration assay from an AMI patient at three consecutive moments t0 - before exercise training (two weeks after randomization) t1 - after exercise training (five weeks after randomization) t2 - after exercise training (seventeen weeks after randomization) The pictures t0 and t1 are partly overlapping details of a larger common picture. In particular the photos show different parts of one migration assay at a single moment, i.e. t0 and t1 are identical. The differences between both photos are accidental - and obviously not due to exercise. Notably the panel does not demonstrate mobilization of stem cells by physical exercise. ![file] ![file] ![file] ,