LncRNA MT1JP inhibits the malignant progression of hepatocellular carcinoma through regulating AKT
Authors: J-C Liu
J-H Liu
J-H Wu
L-L Du
X-S Li
Y-M Su
K Xu
Journal: European review for medical and pharmacological sciences (2020)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: lncRNA MT1JP; hepatocellular carcinoma; expression profile; clinical indicators; prognosis
Affiliations: Postdoctoral Workstation, Qiqihar Institute of Medical Sciences, Qiqihar Medical University, Qiqihar, China. .

Abstract:  Objective: The purpose of this study was to investigate the expression profile of long non-coding RNA (lncRNA) MT1JP in hepatocellular carcinoma (HCC), and to explore the relationship between its expression level and the clinical indicators, as well as the prognosis of HCC patients. Patients and methods: In this study, the expression level of MT1JP in 45 pairs of tumor tissue specimens and paracancerous ones collected from HCC patients were examined through quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) method, and the interplay between MT1JP expression and clinical indicators, as well as the prognosis of HCC patients, was also analyzed. Meanwhile, the expression of MT1JP in HCC cell lines was further verified by qRT-PCR. In addition, MT1JP overexpression model was constructed using lentivirus in HCC cell lines (Hub7 and HepG2), and then, Cell Counting Kit-8 (CCK-8), cell colony formation assay, and flow cytometry were performed to examine the impact of MT1JP on the HCC cell functions. Additionally, whether MT1JP exerts its biological characteristics through protein kinase B (AKT) was finally explored. Results: In this experiment, qRT-PCR results showed that the expression level of lncRNA MT1JP in tumor tissues of HCC patients was remarkably lower than that in adjacent tissues, and the difference was statistically significant. Meanwhile, compared with patients with high expression of MT1JP, patients with low expression of MT1JP had a higher pathological staging and a lower overall survival rate. In addition, overexpression of MT1JP remarkably attenuated the proliferation ability of HCC cells but enhanced cell apoptosis rate at the same time. Finally, Western blot results revealed that the overexpression of MT1JP may markedly reduce the AKT expression, thereby suppressing the malignant progression of HCC. Conclusions: LncRNA MT1JP expression is remarkably decreased in HCC tumor tissue samples, which is associated with pathological stage and poor prognosis of HCC patients. In addition, MT1JP may inhibit the malignant progression of HCC by downregulating AKT. 

Retracted: No

PubPeer comments:
 As with other papers by this group of authors this article is a combination of nonsensical text, vaguely described experiments, and colorful yet meaningless drawings that do not prove anything. ![file] I have many questions. * The authors investigate the  effects of structures within the earthu2019s core by putting chicken eggs in incubators and measuring their waves. Could the authors please clarify how these two things are related? * How did the authors create a condition of microgravity? Did they drop the incubator from a plane? That might have resulted in some cracked eggs, wouldn t it? Or, did they put the eggs in a bed with their feet up, in a slightly tilted position? Or, did they bring the eggs to the International Space Station? I would love to replicate this experiment, but some essential information appears to be missing here. * Which temperature were the eggs incubated at? * Then, the eggs were broken, and their waves were measured by a scope. Which wavelength? What was the brand of the scope? What was the gain? Some specifics appear to be missing here. * In figure 8, a legend appears to be missing. I see a red and a blue line. Which one is which? * The experiment was running for 17 days (408h). Yet, the measurements in Figure 8 appear to stop at 325h. * What would the authors make out of the fact that in Figure 8 the two lines merge after 300h? What is the deeper meaning in terms of the earth core s evolution? ![file] , 
 Let me be the first to propose sending a probe to the earth s core to retrieve some of the stringy black DNA that governs its metabolism. ,