<p>Long Noncoding RNA LINC00460 Promotes Hepatocellular Carcinoma Progression via Regulation of miR-342-3p/AGR2 Axis</p>
Authors: He Wang
Xuan Zhu
Kun Li
Jian Chen
Jing Yang
Xiaoxiong Hu
Journal: OncoTargets and Therapy (2020)
Discipline: CLINICAL MEDICINE
Keywords: AGR2; LINC00460; LncRNA; hepatocellular carcinoma; invasion
Affiliations: Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China., Department of Gastroenterology and Hepatology, The People's Hospital of Yichun City, Yichun 336000, People's Republic of China., Department of General Surgery, The People's Hospital of Yichun City, Yichun 336000, People's Republic of China., Department of Oncology, The People's Hospital of Yichun City, Yichun 336000, People's Republic of China., Clinic Research Center of People's Hospital of Yichun City, Yichun 336000, People's Republic of China., Department of Infection Disease, The People's Hospital of Yichun City, Yichun 336000, People's Republic of China.

Abstract:  Background: Hepatocellular carcinoma (HCC) is the leading cause of cancer-related death worldwide. LINC00460, a novel long non-coding RNA (lncRNA), was recently confirmed as an oncogene in various cancers. However, the biological function and underlying mechanism of LINC00460 in HCC is largely obscure. Methods: Fifty pairs of tumor tissue and adjacent normal tissues from HCC patients, as well as six HCC cell lines and a normal human hepatic epithelial cell line were subjected to qRT-PCR assay to evaluate the expression levels of LINC00460. CCK-8 assays were used to detect the proliferation of HCC cells. Transwell assay was used to measure the migration and invasion abilities of HCC cells. RNA pull-down and luciferase assays were performed to verify the direct interaction between LINC00460 and miR-342-3p. A xenograft model of HCC was established to validate the in vivo function of LINC00460 in HCC progression. Results: We firstly detected LINC00460 expression was significantly upregulated in both HCC tumor tissues and cell lines. The upregulation of LINC00460 was positively associated with HCC progression. Functionally, LINC00460 facilitated HCC cell proliferation, migration, and invasion capacities, which due to that LINC00460 could physically bind to and repress miR-342-3p to elevate the expression of AGR2. Conclusion: Our data firstly reveal the clinical relevance, biological function, and regulatory mechanism of LINC00460 in HCC development. LINC00460 promotes HCC progression by elevating AGR2 expression via sponging miR-342-3p, providing a promising therapeutic target for HCC treatment. 

Retracted: No

PubPeer comments:
 Please compare with  A new application of Schru00f6dinger-type identity to singular boundary value problem for the Schru00f6dinger equation [(Meng 2019)] in the same journal. Large sections of the argument seem to be the same. Here is some comparisons, with He Pang at left, Meng at right: ![file] ![file] Then the two papers diverge, with Meng swerving off suddenly in a new direction with novel previously unmentioned functions, as if everything from (2.3) had wandered in from somewhere else. ![file] Later the papers more-or-less converge, despite the different contexts. ![file] , 
 Please consider these context-setting paragraphs, with my apology for its length. ![file] Beginning at the bottom, with Zhang et al [1] - the references are **not** in order of first use. [1] is Zhang, X., Liu, D., Yan, Z., Zhao, G., Yuan, Y.:  Schru00f6dinger-type identity for Schru00f6dinger free boundary problems . [Bound. Value Probl. 2018, 135 (2018)]( ). I have [concerns about the paper] , but the point here is that the authors do **not** mention kernel functions or Greenu2019s functions, nor do they  give three examples . Nor do they mention  The classical Randon transform (see [17]) as  defined by the Cauchy principal value of the singular integral , but that s fine because it serves no further role in the present paper. The brief summaries in the previous paragraph for Refs [7] to [16] are more accurate, since they are copy-pasted directly from those papers Abstracts. The problem here is that they bear no obvious connection to the present line of argument or conclusions. Moving along to the next paragraph: ![file] The  value of the vaccination-isolation reproductive number makes more sense [in its original context] : ![file] ,