TRIM59 suppresses NO production by promoting the binding of PIAS1 and STAT1 in macrophages
Authors: Qianjing Zhang
Xiaomin Su
Yuan Zhang
Rongcun Yang
Jianmei Yue
Yachen Wang
Journal: International Immunopharmacology (2020)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: COX2; JAK2-STAT1 pathways; Macrophage; Melanoma; TRIM59
Affiliations: Department of Immunology, Nankai University School of Medicine, Nankai University, Tianjin, China; Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin, China; State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China. Electronic address: ., Department of Immunology, Nankai University School of Medicine, Nankai University, Tianjin, China; Key Laboratory of Bioactive Materials Ministry of Education, Nankai University, Tianjin, China; State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China., Department of Immunology, Nankai University School of Medicine, Nankai University, Tianjin, China.

Abstract:  Macrophages, which can secret various inflammation mediators, have an essential role in tumor growth and metastasis. However, the mechanism(s) to regulate the production of inflammation mediator is not completely clear. Here we found that TRIM 59 could inhibit the production of NO and the expression of inducible nitric oxide synthase (iNOS), cytochrome c oxidase subunit2 (COX2) and TNFα. TRIM59 mediated suppression on nitric oxide (NO) production is through inhibiting the activation of JAK2-STAT1 signal pathway. In response to LPS, TRIM59 in macrophages was translocated from cytoplasm to nucleus and directly bound with STAT1. During this process, TRIM59 could recruit much more PIAS1 to bind with STAT1 to suppress the activation of STAT1. Finally, TRIM59 modified macrophages could promote tumor growth. Thus, TRIM59 mediated suppression on NO production by promoting the binding of PIAS1 and STAT1 in macrophages may regulate tumor growth. 

Retracted: No

PubPeer comments:
 This paper has a March 2020 retraction, found at: https: www.pnas.org content early 2020 03 26 2005286117 The text of the retraction notice reads as follows: The authors wish to note the following: u201cIt came to our attention after publication that the fundus images in Fig. 3E and SI Appendix, Fig. S4C of our PNAS article were a duplication of the photos in a recent Aging Cell article (1). These photographs were generated by Dr. Daniel Chen and Dr. Dorota Skowronska-Krawczyk in the course of conducting research exploring the effects of Elovl2 deficiency in the mouse eye at Dr. Kang Zhangu2019s laboratory at University of California San Diego. In the same time period, the authors of the PNAS paper were working at the same laboratory exploring the effects of the Elovl2 loss in the entire mouse body. While the two groups of researchers were working concurrently at Dr. Zhangu2019s University of California San Diego laboratory, the fundus photographs were inadvertently misidentified and mixed in with materials developed for the Elovl2 mouse strain in the PNAS paper, during the process of data organization and presentation. The similarities in genetic properties between the Elovl2 mouse strains contributed to the confusion and subsequent mix-up. u201cWe apologize to the scientific community for this inadvertent error. Despite the fact that this is an honest mistake and the inclusion of the erroneously misidentified photos did not affect the conclusions of the PNAS article nor the validity of the remaining data and findings in the paper, including all of the data generated from the laboratories of Drs. Qi Zhou, Wei Li, and Jian-Kang Zhu, who are unaccountable for this inadvertent error, we believe that a retraction is appropriate to prevent confusion among the scientific community. The affiliation of Dr. Xin Li needs to be corrected as University of California, San Diego, rather than Whitehead Institute for Biomedical Research.u201d , 
 https: twitter.com DrDorotaSK status 1345873497533288448 ,