LncRNA CASC15 functions as an oncogene by sponging miR-130b-3p in bladder cancer
Authors: M-W Wang
C-L Han
Q-H Xia
X Yu
X-B Jin
Y Jin
Z-L Wang
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: lncRNAs; CASC15; bladder cancer; RT-qPCR; miR-130b-3p
Affiliations: Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China. .

Abstract:  Objective: Recent studies have revealed that long noncoding RNAs (lncRNAs) are dysregulated in malignant tumors and participates in carcinogenesis. The purpose of our study was to uncover the mechanisms underlying lncRNA CASC15 in bladder cancer (BLCA). Patients and methods: In this research, Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was performed to detect cancer susceptibility candidate 15 (CASC15) expression in BLCA samples and cells. Besides, the wound healing assay and transwell assay were performed in BLCA cells after CASC15 was knocked down. Furthermore, the bioinformatics analysis and dual-luciferase reporter assay were conducted to explore the target miRNA of CASC15, which was further verified through rescue experiments in BLCA cells. Results: CASC15 expression was upregulated in BLCA tissue samples. Moreover, CASC15 downregulated the miR-130b-3p expression and promoted cell migration and invasion in BLCA in vitro. The rescue experiments also revealed that the inhibitory effects by the silence of CASC15 could be reversed through the inhibition of miR-130b-3p. Conclusions: Our study suggested a vital regulatory mechanism of CASC15 in BLCA, and the CASC15/miR-130b-3p axis might serve as a new therapeutic interventional target for BLCA patients. 

Retracted: Yes

PubPeer comments:
 This paper is one of a set of 121 papers that all contain images from the same library of about 100 photos and plots. This is unexpected, because each paper has different authors and different affiliations, with little to no overlap, and describes different cancer types and molecules. It would be very hard to explain how authors from different labs would all obtain the same photo. Therefore, the papers might have all be derived from the same source, such as a paper mill that sells ready-to-be-published papers. This  Stock Photo paper set has been described in more detail on my blog post, https: scienceintegritydigest.com 2020 07 05 the-stock-photo-paper-mill and in a Wall Street Journal article by Eva Xiao (currently behind a paywall): https: www.wsj.com articles chinese-research-papers-raise-doubts-fueling-global-questions-about-scientific-integrity-11593939600 The vast majority (113 121) of the papers were published in the same journal, European Review for Medical and Pharmacological Sciences (ERMPS). A link to the full set of papers can be found here: https: docs.google.com spreadsheets d 172RWS18w1lTNJ9Nnj80fuXXTqTm4fGkoK0sdYyvKE3g edit?usp=sharing All papers have been reported to the journal editors on July 2, 2020. , 
 The publishers have wished the paper into the cornfield: https: www.europeanreview.org article 21853 Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cLncRNA CASC15 functions as an oncogene by sponging miR-130b-3p in bladder cancer, by X. Yu, Z.-L. Wang, C.-L. Han, M.-W. Wang, Y. Jin, X.-B. Jin, Q.-H. Xia, published in Eur Rev Med Pharmacol Sci 2019; 23 (22): 9814-9820u2013DOI: 10.26355 eurrev_201911_19544u2013PMID 31799648u201d has been withdrawn. The Publisher apologizes for any inconvenience this may cause. Links to the original PDF no longer function, but a copy was archived at the Wayback Machine: https: web.archive.org web 20200209145441 https: www.europeanreview.org wp wp-content uploads 9814-9820.pdf , 
  [Decision on handling misconduct cases investigated and handled in 2021 (second batch)](http: www.nsfc.gov.cn publish portal0 tab434 info81402.htm) [Google Translate] ------------------------------------------------------- Regarding the purchase of experimental data and writing on behalf of Xiao and other published papers Proxy investment, data falsification, false signature, unauthorized bidding of fund project numbers of others And other issues The Supervision Committee of the National Natural Science Foundation of China reviewed the retracted papers of Shandong University Yu Xiao and others  X Yu, ZL Wang, CL Han, MW Wang, Y Jin, XB Jin*, QH Xia*. LncRNA CASC15 functions as an oncogene by sponging miR -130b-3p in bladder cancer. European Review for Medical and Pharmacological Sciences. 2019, 23(22):9814-9820.u201d (labeled with fund number 81572534) The organization conducted an investigation on suspected academic misconduct. Upon investigation, Yu Xiao, the first author of the paper, purchased experimental data from a third-party company at his own expense and entrusted him to write the paper on behalf of him. The paper had a data fraud problem. In addition, Yu Xiao also signed others as the author without authorization, and marked others with the project number of the National Natural Science Foundation of China without authorization. ,