MicroRNA‐205 affects mouse granulosa cell apoptosis and estradiol synthesis by targeting CREB1
Authors: Xiaohui Liu
Chengcheng Tan
Weixia Wang
Hongyan Lang
Yumin Zhao
Xintao Li
Pengju Zhang
Jun Wang
Xinghong Wu
Haiyan Liu
Journal: Journal of Cellular Biochemistry (2018)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: apoptosis; cyclic AMP response element (CRE)-binding protein 1 (CREB1); estradiol synthesis; follicular atresia; miR-205.
Affiliations: Institute of Animal Sciences, Jilin Academy of Agricultural Sciences Changchun Jilin China, College of Animal Science and Technology, Jilin Agricultural University Changchun Jilin China

Abstract:  MicroRNA-205 (miR-205) is involved in various physiological and pathological processes, but its biological function in follicular atresia remains unclear. In this study, we investigated miR-205 expression in mouse granulosa cells (mGCs) and analyzed its functions in primary mGCs by performing a series of in vitro experiments. Quantitative real-time polymerase chain reaction showed that miR-205 expression was significantly higher in early atretic follicles and progressively atretic follicles than in healthy follicles. miR-205 overexpression in mGCs significantly promoted apoptosis and caspase-3/9 activities, as well as inhibited estrogen (E2) release and cytochrome P450 family 19 subfamily A polypeptide 1 (CYP19A1, a key gene in E2 production) expression. Bioinformatics and luciferase reporter assays revealed that the gene encoding cyclic AMP response element (CRE)-binding protein 1 (CREB1) was a direct target of miR-205 in mGCs. CREB1 upregulation partially rescued the effects of miR-205 on apoptosis, caspase-3/9 activities, E2 production, and CYP19A1 expression on mGCs. These results indicate that miR-205 might play an important role in ovarian follicular development and provide new insights into follicular atresia. 

Retracted: No

PubPeer comments:
 Fig 3C, 5B.  The .. protein expression levels were analyzed in mGCs transfected with miRu2010205 mimics or miRu2010NC by ... Western blot ;  CREB1, Bax, Bclu20102, and CYP19A1 protein expression was determined in mGCs transfected with miRu2010205 or the miRu2010NC plasmid, with or without the CREB1u2010overexpressing plasmid (without 3u2032u2010UTR) . ![file] If the CYP19A1 band is being reused here to illustrate different points, shouldn t the loading-control band be duplicated as well? UPDATE: *[left]* Fig 5D from [Xia et al (2018)] .  The MMP-9 expression on ... protein levels were determined in A549 cells transfected with si-NC, si-HOXD-AS1, and si-HOXDAS1+miR-133b inhibitor . *[right]* Fig 6A from [Zhao et al (2018)] .  STAT3, CyclinD1, Bcl-2 and MMP-9 protein expression were determined by western blot in RA-FLS after the transfection of pCDNA3.1-GRIM19 and pCDNA3.1. ![file] , 
 *[right]* Fig 2B.  Cell apoptosis was determined in mGCs transfected with miR-205 mimics or miR-NC by flow cytometric analysis . *[left]* Fig 3A from [Li et al (2019)] .  Cell apoptosis was detected in RA-FLSs transfected with miR-506 mimic or miR-NC mimic by flow cytometric analysis. ![file] Could the authors of either paper comment on the appearance of the same flow-cytometry outputs in both? ,