Unique Features of Cortical Bone Stem Cells Associated With Repair of the Injured Heart
Authors: Steven R Houser
Elorm J Agra
Sadia Mohsin
Yan Zhou
Hajime Kubo
Thomas E Sharp
Neil Zalavadia
Shavonn Smith
Timothy Starosta
Constantine D Troupes
Jason M Duran
Remus M Berretta
Journal: Circulation Research (2015)
Discipline: CLINICAL MEDICINE
Keywords: adult stem cells; engraftment; histamine; paracrine factors proliferation; survival.
Affiliations: From the Cardiovascular Research Center, Temple University School of Medicine, Philadelphia, PA (S.M., C.D.T., T.S., T.E.S., E.J.A., S.S., J.M.D., N.Z., H.K., R.M.B., S.R.H.); and Biostatistics and Bioinformatics Facility, Fox Chase Cancer Center, Philadelphia, PA (Y.Z.)., From the Cardiovascular Research Center, Temple University School of Medicine, Philadelphia, PA (S.M., C.D.T., T.S., T.E.S., E.J.A., S.S., J.M.D., N.Z., H.K., R.M.B., S.R.H.); and Biostatistics and Bioinformatics Facility, Fox Chase Cancer Center, Philadelphia, PA (Y.Z.). .

Abstract:  Rationale: Adoptive transfer of multiple stem cell types has only had modest effects on the structure and function of failing human hearts. Despite increasing the use of stem cell therapies, consensus on the optimal stem cell type is not adequately defined. The modest cardiac repair and functional improvement in patients with cardiac disease warrants identification of a novel stem cell population that possesses properties that induce a more substantial improvement in patients with heart failure. Objective: To characterize and compare surface marker expression, proliferation, survival, migration, and differentiation capacity of cortical bone stem cells (CBSCs) relative to mesenchymal stem cells (MSCs) and cardiac-derived stem cells (CDCs), which have already been tested in early stage clinical trials. Methods and results: CBSCs, MSCs, and CDCs were isolated from Gottingen miniswine or transgenic C57/BL6 mice expressing enhanced green fluorescent protein and were expanded in vitro. CBSCs possess a unique surface marker profile, including high expression of CD61 and integrin β4 versus CDCs and MSCs. In addition, CBSCs were morphologically distinct and showed enhanced proliferation capacity versus CDCs and MSCs. CBSCs had significantly better survival after exposure to an apoptotic stimuli when compared with MSCs. ATP and histamine induced a transient increase of intracellular Ca(2+) concentration in CBSCs versus CDCs and MSCs, which either respond to ATP or histamine only further documenting the differences between the 3 cell types. Conclusions: CBSCs are unique from CDCs and MSCs and possess enhanced proliferative, survival, and lineage commitment capacity that could account for the enhanced protective effects after cardiac injury. 

Retracted: No

PubPeer comments:
 Fig3E: Same microscopy images for different cell types and treatments, in **red**. Contrast slightly adjusted. ![file] , 
 #1 I independently came across this today and agree this image appears to be repeated. Could the authors please respond? Sources of Funding S.R. Houser is funded by National Institutes of Health and American Heart Association. S. Mohsin is funded by American Heart Association (Scientific Development Grant). , 
 Originally published 5 Nov 2020 In the article by Mohsin et al, u201cUnique Features of Cortical Bone Stem Cells Associated With Repair of the Injured Heart,u201d which published in the December 4, 2015 issue of the journal (Circ Res. 2015;117:1024u20131033, doi: https: doi.org 10.1161 CIRCRESAHA.115.307362. One of the thirty panels in Figure 3E (the image of Cardiac derived cells in an undifferentiated state [CDCs UD] stained for IGF) was not correctly placed in the figure. The correct panel was accidently deleted during figure preparation leaving the image used for sizing all thirty panels. The correct panel is now included in the new Figure 3E. ![file] The authors apologize for this error. ,