Anti-apoptotic effect of microRNA-30b in early phase of rat myocardial ischemia-reperfusion injury model
Authors: Qian Li
Jin-Peng Wang
Xin Xue
Ji-Chang Zhang
Jia Liu
Yun-Peng Yu
Li-Yan Zhao
Yong-Feng Shi
Jun-Duo Wu
Chun-Li Song
Bei-Lin Zhang
Guan Wang
Yang-Xue Li
Zhi-Xian Liu
Hong-Ying Diao
Bin Liu
Dan Cai
Journal: Journal of Cellular Biochemistry (2015)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: ANTI-APOPTOSIS; BAX; BCL-2; CASPASE-3; ISCHEMIC-REPERFUSION INJURY
Affiliations: Department of Cardiology, the Second Hospital of Jilin University, Changchun, 130041, P. R. China., Department of Physiology, the College of Basic Medical Sciences of Jilin University, Changchun, 130021, P. R. China., Department of Clinical Laboratory, the Second Hospital of Jilin University, Changchun, 130041, P. R. China.

Abstract:  This study aimed to investigate the effect of microRNA-30b (miR-30b) in rat myocardial ischemic-reperfusion (I/R) injury model. We randomly divided Sprague-Dawley (SD) rats (n = 80) into five groups: 1) control group; 2) miR-30b group; 3) sham-operated group; 4) I/R group, and 5) I/R+miR-30b group. Real-time quantitative polymerase chain reaction, immunohistochemical staining and Western blot analysis were conducted. TUNEL assay was employed for testing cardiomyocyte apoptosis. Our results showed that miR-30b levels were down-regulated in I/R group and I/R + miR-30b group compared with sham-operated group (both P &lt; 0.05). However, miR-30b level in I/R + miR-30b group was higher than I/R group (P &lt; 0.05). Markedly, the apoptotic rate in I/R group showed highest in I/R group (P &lt; 0.05). Additionally, the results illustrated that protein levels of Bcl-2, Bax, and caspase-3 were at higher levels in ischemic regions in I/R group, comparing to sham-operated group (all P &lt; 0.05), while Bcl-2/Bax was reduced (P &lt; 0.05). Bcl-2 level and Bcl-2/Bax were obviously increased in I/R + miR-30b group by comparison with I/R group, and expression levels of Bax and caspase-3 were down-regulated (all P &lt; 0.05). We also found that in I/R + miR-30b group, KRAS level was apparently lower and p-AKT level was higher by comparing with I/R group (both P &lt; 0.05). Our study indicated that miR-30b overexpression had anti-apoptotic effect on early phase of rat myocardial ischemia injury model through targeting KRAS and activating the Ras/Akt pathway. 

Retracted: Yes

PubPeer comments:
 Figure 6A. Repeated bands. The authors seems to accidentally use the bands representing other results to represent the results of Bax and or Casepase-3. In addition, I m curious why the background is grid-like, which seems to be different from the results shown by other researchers. ![file] , 
 Retracted [22 September 2021] . -------------------- The above article, published online on 28 April 2015 in Wiley Online Library has been retracted by agreement between the authors, the journal s Editor in Chief, Prof. Dr. Christian Behl, and Wiley Periodicals LLC. The retraction has been agreed because the authors stated that unintentional errors occurred during the research process, and the experimental results cannot be verified. Thus, the conclusions are considered to be invalid. The authors apologize for the unintentional errors. ,