<p>Long Noncoding RNA LINC00839 Promotes the Malignant Progression of Osteosarcoma by Competitively Binding to MicroRNA-454-3p and Consequently Increasing c-Met Expression</p>
Authors: Xiaoyong Chen
Guangdong Chen
Li Ma
Hai Guo
Yang Zhang
Journal: Cancer Management and Research (2020)
Discipline: CLINICAL MEDICINE
Keywords: ceRNA; long intergenic non-protein coding RNA 839; therapeutic target.
Affiliations: Department of Orthopedics, Shenzhen University General Hospital, Shenzhen 518055, People's Republic of China., Department of Anesthesiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830000, People's Republic of China., Department of Emergency, General Hospital of Xinjiang Military Command of Chinese People's Liberation Army, Urumqi, Xinjiang, 830000, People's Republic of China., Department of Orthopedics, Cangzhou Center Hospital, Cangzhou, Hebei 061014, People's Republic of China.

Abstract:  Purpose: This study was conducted to determine the expression and prognostic relevance of long intergenic non-protein coding RNA 839 (LINC00839) in osteosarcoma (OS) and to explore the detailed roles of LINC00839 in regulating OS cell activities and the mechanisms responsible for its cancer-promoting activity in OS. Methods: The expression of LINC00839 in OS tissues and cell lines was determined by quantitative reverse transcription-polymerase chain reaction. After LINC00839 knockdown, cell counting kit-8 assay, flow cytometric analysis, transwell migration and invasion assay, and in vivo tumor xenograft assay were used to detect its effects on cellular processes in OS. Bioinformatics analyses were conducted to predict the putative miRNAs that target LINC00839. RNA immunoprecipitation assay, luciferase reporter assay, Western blotting analysis, and rescue assays were conducted to establish a relationship among LINC00839, microRNA-454-3p (miR-454-3p), and cellular mesenchymal to epithelial transition factor (c-Met) in OS. Results: LINC00839 was upregulated in OS tissues and cell lines. OS patients characterized with high LINC00839 expression exhibited shorter overall survival than patients with low LINC00839 expression. LINC00839 knockdown caused a significant reduction in OS cell proliferation, migration, and invasion in vitro. Furthermore, LINC00839 depletion inhibited OS tumor growth in vivo and induced apoptosis. Mechanistically, LINC00839 functions as a competitive endogenous RNA in OS by sponging miR-454-3p. c-Met was confirmed as a direct target gene for miR-454-3p in OS cells and was positively regulated by LINC00839 by competitively binding to miR-454-3p. Conclusion: LINC00839 promoted the oncogenicity of OS by targeting the miR-454-3p/c-Met axis. The LINC00839/miR-454-3p/c-Met network may represent a potential target for OS therapy. 

Retracted: Yes

PubPeer comments:
 In Figs 1F, 4C, 5C, a panel seems to be derived from the same experimental data as panels in two other papers. Here, the right-hand panel. I have adjusted aspect ratios and contrast to facilitate comparison. ![file] In context: ![file] Other panels are from Fig 4B of [Li et al (2019)] , and Fig 1I of [Yue Wang (2020)] . ![] ![file] , 
 *[left]* Fig 6A,  Representative images of tumor xenografts originating from sh-LINC00839 or sh-NC stably transfected HOS cells . *[right]* Fig 9B from [Dong et al (2020)] ,  The tumor growth rate was significantly suppressed by sh-LINC00324, as compared with that of the sh-NC group. ![file] The decision of separate research groups to photograph their tumor collections in such a similar style would not be suspicious in itself, but it sits alongside the many other similarities within this corpus of papers. , 
 Retracted [18 October 2021] . ------------------------- The Editor and Publisher of Cancer Management and Research wish to retract the published article. Concerns were raised over alleged image duplication in Figure 5C with similar images from unrelated articles, specifically: - Figure 5C panel HOS si-LINC00839+pcDNA3.1 appears to have been duplicated with similar images in Figure 1I panel MDA-MB-231 si-LINC00922 from Yue and Wang, 2020 and Figure 4B panel agomir-483-3p from Li et al, 2019 . The authors did not respond to our queries and the Editor determined the findings of the study were no longer valid and advised for the article to be retracted. Our decision-making was informed by our policy on publishing ethics and integrity and the COPE guidelines on retraction. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as u201cRetractedu201d. ,