MiR-210 suppresses neuronal apoptosis in rats with cerebral infarction through regulating VEGF-notch signaling pathway
Authors: W-W Liu
Y Wang
W-Y Yang
Y-L Jiang
Journal: European review for medical and pharmacological sciences (2020)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: miR-210; neuronal apoptosis; VEGF; Notch signaling pathway; rats
Affiliations: Institute of Forensic, Kunming Medical University, Kunming, China. .

Abstract:  Objective: The aim of this study was to explore the effect of micro ribonucleic acid (miR)-210 on neuronal apoptosis in rats with cerebral infarction (CI) by regulating the vascular endothelial growth factor (VEGF)-Notch signaling pathway. Materials and methods: A total of 30 clean healthy male Sprague-Dawley rats weighing 200-300 g were selected and randomly divided into Sham group (n=10), CI model group (CIM group, n=10), and CIM + miR-210 Mimic group (n=10). The protein expression levels of VEGF, Notch1, cleaved-Caspase3 (c-Caspase3), B-cell lymphoma-2 (Bcl-2), and tubulin were detected via Western blotting. The messenger RNA (mRNA) levels of VEGF and Notch1 were detected via quantitative Polymerase Chain Reaction (qPCR). Meanwhile, the expression levels of VEGF and Notch1 in tissues were determined using immunohistochemistry. Furthermore, the apoptosis of tissues was determined via Annexin V-FITC, propidium iodide (PI) double labeling, and flow cytometry. Results: The levels of VEGF and Notch1 increased significantly in the CIM group when compared with those in the Sham group (p&lt;0.01). However, their expressions decreased remarkably in CIM + miR-210 Mimic group when compared with CIM group (p&lt;0.05). The mRNA expressions of VEGF and Notch1 were evidently upregulated in the CIM group when compared with the Sham group (p&lt;0.01), whereas they were remarkably downregulated in the CIM + miR-210 Mimic group than CIM group (p&lt;0.05). Immunohistochemistry results indicated that the expression levels of VEGF and Notch1 in tissues were consistent with Western blotting results. Besides, the protein expressions of c-Caspase3 and Bcl-2 were remarkably higher in the CIM group than Sham group (p&lt;0.01). However, they were significantly lower in the CIM + miR-210 Mimic group than those in the CIM group (p&lt;0.05). In addition, flow cytometry results demonstrated that the apoptosis level increased significantly in CIM group when compared with the Sham group (p&lt;0.05), while it was remarkably inhibited in the CIM + miR-210 Mimic group (p&lt;0.05). Conclusions: MiR-210 can reduce the protein expressions of VEGF and Notch1, inhibit the VEGF-Notch signaling pathway, decrease the expression of pro-apoptotic factor c-Caspase3 and increase the expression of anti-apoptotic factor Bcl-2, thereby suppressing cerebral neuronal apoptosis and preventing CI-induced neuronal apoptosis. 

Retracted: No

PubPeer comments:
 Figure 5.  Effect of miR-210 on apoptosis level in each group. A, Apoptosis detected via Annexin V-FITC and PI double labeling . ![file] Figure 4 from [Yu et al (2020)] .  Effect of miR-146 on apoptosis of kidney tissues. A, Apoptosis determined using Annexin V-FITC and PI dualfluorescence labeling and flow cytometry . ![file] , 
 #1 Fig 5 again - now compared with (below) three panels from Fig 11 of [Zhang et al (2018)] ,  Apoptosis rate of cardiomyocytes in each group after transfection. Notes: A, apoptotic scatter plot . ![file] Similarities are greatest in the left-hand quadrants of each pair. , 
 Expression of Concern: https: pubmed.ncbi.nlm.nih.gov 32964943 *The Editorial Board of European Review for Medical and Pharmacological Sciences would like to issue an Expression of Concern for  MiR-210 suppresses neuronal apoptosis in rats with cerebral infarction through regulating VEGF-notch signaling pathway , by Y.-L. Jiang, W.-W. Liu, Y. Wang, W.-Y. Yang, published in Eur Rev Med Pharmacol Sci 2020; 24 (9): 4971-4978-DOI: 10.26355 eurrev_202005_21188. PMID: 32432760. Following publication, the authors wrote to the Journal and stated  Part of the experiments in this paper were performed by a third party. Reports on the science integrity of the third-party academic institutions in China made us aware of the reliability and originality of the data provided by them. The matter has been referred to our institution for further investigation . Hence, the Editorial Office of European Review for Medical and Pharmacological Sciences decided to publish an expression of concern to notify readers while the investigation is underway. The Publisher apologizes for any inconvenience this may cause.* ---------------------------------------- Retracted. https: www.europeanreview.org article 24300 *This Retraction follows an Expression of Concern in Eur Rev Med Pharmacol Sci 2020; 24 (17): 8621-8621u2013DOI: 10.26355 eurrev_202009_22743u2013PMID: 32964943. The article u201cMiR-210 suppresses neuronal apoptosis in rats with cerebral infarction through regulating VEGF-notch signaling pathway, by Y.-L. Jiang, W.-W. Liu, Y. Wang, W.-Y. Yang, published in Eur Rev Med Pharmacol Sci 2020; 24 (9): 4971-4978u2013DOI: 10.26355 eurrev_202005_21188u2013PMID: 32432760u201d has been withdrawn from the authors due to some inaccuracies (Some data cannot be repeated by our further research).* *The Publisher apologizes for any inconvenience this may cause.* ![file] ,