LncRNA n335586/miR-924/CKMT1A axis contributes to cell migration and invasion in hepatocellular carcinoma cells
Authors: Xiaopei Zhao
Hongxia Fan
Min Liu
Yujie Feng
Jing Lv
Ping Lv
Yankun Liu
Hua Tang
Ting Mu
Journal: Cancer Letters (2018)
Discipline: CLINICAL MEDICINE
Keywords: CKMT1A; HCC migration/invasion; ceRNA; lncRNA n335586; miR-924.
Affiliations: Tianjin Life Science Research Center and Department of Pathogen Biology, Collaborative Innovation Center of Tianjin for Medical Epigenetics, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China., Tianjin Life Science Research Center and Department of Pathogen Biology, Collaborative Innovation Center of Tianjin for Medical Epigenetics, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China; The Cancer Institute, Tangshan People's Hospital, Tangshan, China., Tianjin Life Science Research Center and Department of Pathogen Biology, Collaborative Innovation Center of Tianjin for Medical Epigenetics, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China. Electronic address: .

Abstract:  Hepatocellular carcinoma (HCC) is a leading cause of cancer death worldwide and chronic hepatitis B virus (HBV) infection is a major risk factor for HCC. Emerging evidences indicate that long noncoding RNAs (lncRNAs) play a pivotal role in HCC development, but its contribution to HBV-related HCC remains largely unclear. Differentially expressed lncRNAs in HBV-related HCC tissues were identified by deep sequencing in our previous study. The function of lncRNA n335586, one of most up-regulated lncRNAs in HBV-related HCC, was characterized in the present study. We found that the expression of n335586 was significantly increased in HBV positive HCC tissues and cells and was induced by HBV in vitro. Function study indicated that lncRNA n335586 remarkably promoted HCC cells migration, invasion and epithelial-mesenchymal transition (EMT) in vitro and metastasis in vivo. Further mechanistic studies showed lncRNA n335586 promoted HCC cells migration and invasion through facilitating the expression of its host gene CKMT1A by competitively binding miR-924. In conclusion, we demonstrated that the n335586/miR-924/CKMT1A axis contributes to HCC cell migration and invasion, which may be helpful for understanding of pathogenesis of HBV-related HCC. 

Retracted: No

PubPeer comments:
 Figure 7C appears to include some image overlaps. *** ![file] *** ![file] , 
 Dear Actinopolyspora Biskrensis, ![file] Thank you very much for pointing out this error. We checked the data. During the preparation of figures submitted in the original manuscript, we inadvertently provided an incorrect image for Figure 7C. In the figure 7C: For migration, pcDNA3+pcDNA3 group is correct, pri-miR-924+n335586 group was wrong image by mistake, and is updated with correct image. For invasion, pcDNA3+pcDNA3 group is correct, pri-miR-924+n335586 group was wrong image by mistake, and is updated with correct images. The corrected versions of Fig. 7C and Fig. 7 were provided below. The correction did not affect the overall conclusions and we will make the corrigendum. We apologize for this mistake due to our carelessness. Beat regards, Hongxia Fan, Hua Tang ![file] , 
 I appreciate your response, and look forward to hearing how the correction is processed by the journal. , 
 Dr. Tang, could you also please check Figure 4B? There appears to be another duplication, marked with pink boxes. ![file] , 
 Corrigendum, December 2020, found here: https: www.sciencedirect.com science article pii S0304383520303748  *The authors regret In the original version of the article, the WB band for E-cadherin in Fig. 2G, the WB band for cytokeratin and Vimentin in Fig. 2H were placed by mistake. The transwell invasion image for CKMT1A in Fig. 4B and the WB band for E-cadherin in Fig. 4D were placed by mistake. The transwell migration and invasion images for pri-miR-924 + n335586 in Fig. 7C were placed by mistake. The corrected version of Fig.2, Figs. 4 and 7 were provided below. These corrections do not change the results or conclusions of this paper. We sincerely apologize for the errors due to our carelessness. * *The authors would like to apologize for any inconvenience caused.* ,