Herbometallic nano-drug inducing metastatic growth inhibition in breast cancer through intracellular energy depletion
Authors: Kunal Pal
Sutapa Som Chaudhury
Bhuban Ruidas
Prasanta Kumar Sarkar
Pritha Das
Chitrangada Das Mukhopadhyay
Tapas Kumar Sur
Koel Sinha
Parash Prasad
Journal: Molecular Biology Reports (2020)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Antitumor; Apoptosis; Glyco-stress; Herbometallic; Metastasis
Affiliations: Centre for Healthcare Science and Technology, Indian Institute of Engineering Science Technology, Shibpur, Howrah, West Bengal, 711103, India., Department of Pharmacology, R G Kar Medical College and Hospital, Kolkata, West Bengal, 700004, India., Department of Life Sciences and Biotechnology, Jadavpur University, Kolkata, West Bengal, 700032, India., Cell Biology and Physiology Division, CSIR-Indian Institute of Chemical Biology, Kolkata, West Bengal, 700032, India., Department of Rasashastra, J. B. Roy State Ayurvedic Medical College and Hospital (affiliated to the University of Calcutta), Kolkata, West Bengal, 700004, India., Centre for Healthcare Science and Technology, Indian Institute of Engineering Science Technology, Shibpur, Howrah, West Bengal, 711103, India. .

Abstract:  Cancer cells need extensive energy supply for their uncontrolled cell division and metastasis which is exclusively dependent on neighboring cells, especially adipocytes. Herein, we have introduced a novel herbometallic nano-drug, Heerak Bhasma nanoparticle (HBNP) from natural resources showing high potential in the reduction of energy supply thereby promoting cell death in breast cancer cells. Inductively coupled plasma optical emission spectra (ICP-OES), atomic absorption spectra (AAS), Raman spectra, X-ray diffraction analyses confirmed the physicochemical properties of HBNP. The differential light scattering (DLS) and field emission scanning electron microscope (FESEM) analyzed the cell-permeable size of HBNP, whereas, cell viability assay confirmed the non-toxic effect. Seahorse energy efflux assay, apoptotic cell quantification, ROS, mitochondrial membrane potential, in vivo oxidative stress etc. were measured using standard protocol. The notable changes in cancer energy metabolism investigated by cellular Mito and Glyco-stress analyses confirmed the HBNP induced intracellular energy depletion. Also, a significant reduction in mitochondrial membrane potential and subsequently, extensive reactive oxygen species (ROS) generations were observed in presence of HBNP followed by the induction of cell apoptosis. The cell invasion and wound healing assay followed by reduced expression both protein (MMP 2, MMP 9) and cytokine (IL6, IL10) had signified the effectiveness of HBNP against cancer metastasis. In addition, HBNP also showed an excellent antitumor activity in vivo followed by developing healing characteristics due to oxidative stress. All these findings strongly suggest that HBNP has the potential to be the new cancer therapeutic. A schematic phenomenon represents the overall HBNP mediated anticancer activity via limitation of both fatty acid uptake and energy metabolism. 

Retracted: No

PubPeer comments:
 ![file] The publication  Herbometallic nano-drug inducing metastatic growth inhibition in breast cancer through intracellular energy depletion (10.1007 s11033-020-05467-7) has some images replicated from  Natural product inspired allicin analogs as novel anti-cancer agents (10.1016 j.bioorg.2019.01.057). **Fig. 7(B) Control**, **Fig. 7(B) HBNP 20u00b5g ml**, Fig. 7(B) HBNP 40u00b5g ml of DOI **10.1007 s11033-020-05467-7** are similar to images **Fig. 2 MCF-7 Control**, **Fig. 2 MCF-7 15u00b5M**, **Fig. 2 MCF-7 H2O2** of DOI **10.1016 j.bioorg.2019.01.057** respectively. ,