Inhibition of BAP31 expression inhibits cervical cancer progression by suppressing metastasis and inducing intrinsic and extrinsic apoptosis
Authors: Yanqin Zhang
Peilong Cao
An Wang
Journal: Biochemical and Biophysical Research Communications (2019)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: BAP31; Cervical cancer; Intrinsic and extrinsic apoptosis; Metastasis.
Affiliations: Department of Obstetrics, Ankang Central Hospital, Ankang, 725000, China., Department of Nursing, Yulin Traditional Chinese Medicine Hospital, Yulin, 719000, China., Department of Pathology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, China. Electronic address: .

Abstract:  Cervical cancer is reported as one of the most lethal types of cancer among female. However, extensive studies of the molecular mechanisms that regulate the progression of cervical cancer are still required. B-cell associated protein (BAP)-31 is a 28-kDa integral membrane protein in the endoplasmic reticulum (ER), playing essential role in modulating various physiological processes. The present study indicated that BAP31 was a novel gene associated with cervical cancer development. Here, we demonstrated that BAP31 was significantly increased in human cervical cancer specimens, which was positively correlated to histological grade of the cancer. BAP31 knockdown suppressed cell proliferation, clonogenic ability and metastasis-associated traits in vitro, as well as carcinogenesis and pulmonary metastasis in vivo. Further studies indicated that the expression levels of transforming growth factor (TGF)-β1, matrix metalloproteinase (MMP)-2, MMP-9, Rho-associated protein kinase 1 (ROCK1), α-smooth muscle actin (α-SMA), Vimentin and N-cadherin were markedly reduced by BAP31 knockdown in cervical cancer cells. In addition, intrinsic and extrinsic apoptosis was significantly induced in BAP31 knockdown cells, as evidenced by the increased expression of cleaved Caspase-8/-9/-3 and poly (ADP-ribose) polymerases (PARP). Notably, suppressing the activities of Caspase-8/-9 and -3 obviously diminished BAP31 silence-triggered apoptosis. Together, these findings highlighted an essential role for BAP31 in the modulation of tumorigenesis and metastatic potential of cervical cancer, and demonstrated a promising application of BAP31 in cancer prevention. 

Retracted: No

PubPeer comments:
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 An image in this paper seems to overlap with an image in a different paper and journal; no common authors were noticed. Figure 1J, Biochemical and Biophysical Research Communications (2019), doi: 10.1016 j.bbrc.2018.11.017, discussed here: https: pubpeer.com publications 00620D448446453421FA1436A66337 Figure 2C, Cell Death Disease (2018), doi: 10.1038 s41419-018-0334-2, discussed here: https: pubpeer.com publications 1308B6D5876AD3007912F0F9959C9D *** Brightness enhanced ![file] , 
 An image in this paper seems to overlap with an image in a different paper and journal; no common authors were noticed. Figure 4G, Biochemical and Biophysical Research Communications (2019), doi: 10.1016 j.bbrc.2018.11.017, discussed here: https: pubpeer.com publications 00620D448446453421FA1436A66337 Figure 5e, excerpt, Scientific Reports (2017), doi: 10.1038 s41598-017-09271-5, discussed here: https: pubpeer.com publications 9CFBDEF6838FBE19F5DD0A59F80310 The error bars on the earlier paper do not appear on the images in the later paper, suggesting that whomever prepared these figures may have had access to a clean original. *** ![file] ,