MiR-155 affects proliferation and apoptosis of bladder cancer cells by regulating GSK-3β/β-catenin pathway
Authors: X-X Zhang
H Wu
Z-Q Yao
Z-C Dong
C-H Chen
D Zhang
J-Q Tian
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: GSK-3β; Wnt/β-catenin signaling pathway; miR-155; bladder cancer; proliferation
Affiliations: Department of Urology, Key Laboratory of Gansu Province for Urological Diseases, Department of Urology of Lanzhou University Second Hospital, Urology institute of Lanzhou University Second Hospital, Lanzhou, Gansu, P.R. China. .

Abstract:  Objective: GSK-3β negatively regulates Wnt/β-catenin signaling pathway. The abnormal miR-155 expression is associated with bladder cancer. Bioinformatics analysis revealed a complementary binding site between miR-155 and GSK-3β mRNA. This study investigated the role of miR-155 in the proliferation and apoptosis of bladder cancer cells. Patients and methods: The dual luciferase reporter gene assay validated the targeted regulation between miR-155 and GSK-3β. Tumor tissues and adjacent tissues were collected from bladder cancer patients and the expression of miR-155 and GSK-3β mRNA was detected by RT-PCR. Bladder cancer cell line BIU-87 cells were cultured in vitro and divided into miR-NC group and miR-155 inhibitor group. The expressions of miR-155, GSK-3β and β-catenin were compared, cell apoptosis was detected by flow cytometry, and cell proliferation was detected by EdU staining. Results: Compared with adjacent tissues, miR-155 expression was significantly increased in bladder cancer tissues, and GSK-3β mRNA expression was significantly decreased. There was a targeted regulatory relationship between miR-155 and GSK-3β. Compared with SV-HUC-1 cells, miR-155 expression in bladder cancer BIU-87 and 5637 cells was significantly increased, and GSK-3β expression was significantly decreased. Transfection of miR-155 inhibitor significantly increased GSK-3β expression in BIU-87 and 5637 cells, decreased β-catenin expression, increased cell apoptosis, and decreased cell proliferation. Conclusions: The increased expression of miR-155 plays a role in reducing the expression of GSK-3β and in promoting the pathogenesis of bladder cancer. Inhibition of miR-155 can up-regulate the expression of GSK-3β, inhibit the activity of Wnt/β-catenin pathway, attenuate proliferation and promote apoptosis of bladder cancer cells. 

Retracted: Yes

PubPeer comments:
 Please appreciate the effort I put into creating this Gallery of Unfortunate FACS Plots. Fig 4D [Li et al (2019)] 10.26355 eurrev_201909_18832 Fig 5D [Liu et al (2019)] 10.26355 eurrev_201911_19421 Fig 4E [Du et al (2019)] 10.26355 eurrev_201910_19278 Fig 4D [Dong et al (2019)] 10.26355 eurrev_201907_18305 ![file] Fig 4E [Wu et al (2019)] 10.26355 eurrev_201909_18852 Fig 3C [Cao et al (2019)] 10.26355 eurrev_201908_18531 Fig 4E [Wang et al (2019)] 10.26355 eurrev_201906_18181 Fig 4C [Wei et al (2019)] 10.26355 eurrev_201907_18299 ![file] , 
 #1 These eight papers also display a strange stylised form of Western Blots, where out-of-focus geometrical ellipses hover above a featureless background. Shadowy wisps of smoke hang in the foreground but there is no real-world detail. Did I mention the absence of overlap among the different teams of authors, and their far-flung locations? Figs 3C, 4C [Li et al (2019)] 10.26355 eurrev_201909_18832 Figs 4B, 5B [Liu et al (2019)] 10.26355 eurrev_201911_19421 Figs 3C, 4C [Du et al (2019)] 10.26355 eurrev_201910_19278 Figs 3C, 4C [Dong et al (2019)] 10.26355 eurrev_201907_18305 ![file] Figs 2B, 4C [Wu et al (2019)] 10.26355 eurrev_201909_18852 Figs 2B, 3B [Cao et al (2019)] 10.26355 eurrev_201908_18531 Figs 3B, 4C [Wang et al (2019)] 10.26355 eurrev_201906_18181 Figs 3B, 4B [Wei et al (2019)] 10.26355 eurrev_201907_18299 ![file] , 
 It is also worth comparing Fig 4(E)  EdU staining for cell proliferation with 4(D)  flow detection of cell proliferation from [Wei et al (2019)] . ![file] , 
 Now retracted: https: www.europeanreview.org article 23976 -------------------------------------- Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cMiR-155 affects proliferation and apoptosis of bladder cancer cells by regulating GSK-3u03b2 u03b2-catenin pathway, by Z.-C. Dong, D. Zhang, X.-X. Zhang, Z.-Q. Yao, H. Wu, C.-H. Chen, J.-Q. Tian, published in Eur Rev Med Pharmacol Sci 2019; 23 (13): 5682-5690u2013DOI: 10.26355 eurrev_201907_18305u2013PMID: 31298320u201d has been withdrawn. The Publisher apologizes for any inconvenience this may cause. ,