LncRNA DGCR5 promotes non-small cell lung cancer progression via sponging miR-218-5p
Authors: S-G Guo
H-Z Shu
C-Y Xu
J Wang
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: Non-small cell lung cancer; lncRNA; DGCR5; miR-218-5p; migration
Affiliations: Department of Respiratory Disease, Jinshan Hospital of Fudan University, Shanghai, China. .

Abstract:  Objective: Non-small cell lung cancer (NSCLC) is one of the most ordinary malignant tumors worldwide. Recent researches have proved that long noncoding RNAs (lncRNAs) play vital roles in many diseases. The aim of this study was to investigate the exact function of lncRNA DiGeorge syndrome critical region gene 5 (DGCR5) in the development of NSCLC. Patients and methods: Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was utilized to detect DGCR5 expression in paired NSCLC patients' tissue samples and cell lines. The function of DGCR5 in NSCLC was detected through wound healing assay and transwell assay in vitro. Besides, mechanism assays were conducted to observe the interaction between DGCR5 and microRNA-218-5p (miR-218-5p). Results: DGCR5 was remarkably highly expressed in NSCLC tissues compared to that of adjacent normal tissues. The migration and invasion of NSCLC cells were significantly promoted via overexpression of DGCR5. However, the silence of DGCR5 significantly inhibited NSCLC cell migration and invasion. Moreover, RT-qPCR results revealed that miR-218-5p was down-regulated via overexpression of DGCR5, while miR-218-5p was up-regulated after the knockdown of DGCR5. Further experiments showed that miR-218-5p was a direct target of DGCR5 in NSCLC. Conclusions: DGCR5 enhances NSCLC cell migration and invasion via targeting miR-218-5p, indicating that DGCR5 may be a potential therapeutic target in NSCLC. 

Retracted: Yes

PubPeer comments:
 This paper is one of a set of 121 papers that all contain images from the same library of about 100 photos and plots. This is unexpected, because each paper has different authors and different affiliations, with little to no overlap, and describes different cancer types and molecules. It would be very hard to explain how authors from different labs would all obtain the same photo. Therefore, the papers might have all be derived from the same source, such as a paper mill that sells ready-to-be-published papers. This  Stock Photo paper set has been described in more detail on my blog post, https: scienceintegritydigest.com 2020 07 05 the-stock-photo-paper-mill and in a Wall Street Journal article by Eva Xiao (currently behind a paywall): https: www.wsj.com articles chinese-research-papers-raise-doubts-fueling-global-questions-about-scientific-integrity-11593939600 The vast majority (113 121) of the papers were published in the same journal, European Review for Medical and Pharmacological Sciences (ERMPS). A link to the full set of papers can be found here: https: docs.google.com spreadsheets d 172RWS18w1lTNJ9Nnj80fuXXTqTm4fGkoK0sdYyvKE3g edit?usp=sharing All papers have been reported to the journal editors on July 2, 2020. , 
 Withdrawn. https: www.europeanreview.org article 23355 *Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cLncRNA DGCR5 promotes non-small cell lung cancer progression via sponging miR-218-5p, by J. Wang, H.-Z. Shu, C.-Y. Xu, S.-G. Guo, published in Eur Rev Med Pharmacol Sci 2019; 23 (22): 9947-9954u2013DOI: 10.26355 eurrev_201911_19561u2013PMID: 31799664u201d has been withdrawn.* *The Publisher apologizes for any inconvenience this may cause.* ,