<p>Circular RNA Gprc5a Promotes HCC Progression by Activating YAP1/TEAD1 Signalling Pathway by Sponging miR-1283</p>
Authors: Zhixiang Jian
Guanqun Huang
Ye Lin
Haosheng Jin
Journal: OncoTargets and Therapy (2020)
Discipline: CLINICAL MEDICINE
Keywords: HCC; YAP1/TEAD1; circGprc5a; miR-1283; proliferation.
Affiliations: Department of General Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, People's Republic of China., Department of General Surgery, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou 510700, People's Republic of China.

Abstract:  Background: Circular RNA (circRNA) plays a critical role in tumorigenesis and tumor progression. Many studies indicate that circRNA Gprc5a is significantly upregulated and functions as an oncogene in a variety of cancers. However, the molecular mechanism of circGprc5a in liver cancer remains unclear. Methods: qRT-PCR was used to measure the expression levels of circGprc5a, miR-1283, YAP1 and TEAD1 mRNA in hepatocellular carcinoma (HCC) tissues or cells. YAP1 and TEAD1 protein levels were detected by Western ﻿blot. CCK-8 assay, cell colony formation, BrdU incorporation and Annexin V-FITC/PI assays were performed to analyze the effects of circGprc5a and miR-1283 on cell proliferation and apoptosis. The relationship between circGprc5a, miR-1283, YAP1 and TEAD1 was analyzed using bioinformatic analysis and ﻿luciferase. The tumor changes in mice were detected by in vivo experiments. Results: CircGprc5a was highly expressed in liver cancer, and closely related poor survival of patients with liver cancer. Knockout of circGprc5a inhibited proliferation of HCC and induced apoptosis. CircGprc5a activated the YAP1/TEAD1 signaling pathway by acting as a sponge for miR-1283. Furthermore, overexpression of miR-1283 abolished the promotion of circGprc5a on HCC cells. Therefore, miR-1283 expression correlated negatively with circGprc5a expression yet positively with the expression of YAP1/TEAD1 in liver cancer. Conclusion: ﻿CircGprc5a promoted the development of HCC by inhibiting the expression of miR-1283 and activating the YAP1/TEAD1 signaling pathway. 

Retracted: No

PubPeer comments:
 Atomic model in Protein Data Bank (PDB) ID 7MHY (chain N) does not match cryo-EM map (Electron Microscopy Data Bank, EMD-23836) at least in several regions (three images are shown below). ![file] ![file] ![file] , 
 Thank you for this comment. The regions in question refer to two regions of an antibody was used as a reagent to help determine the structure of the HHAT enzyme. For clarification, the top and middle images shown above refer to the same region (amino acids 115-119 of the light chain [chain N] of antibody 1C06). The bottom image refers to amino acids 50-53 of the light chain (chain N) of antibody 1C06. We had difficulty modeling these regions. Because the antibody was used as a tool to help determine the structure of HHAT, the conformations of the amino acids of the antibody are not relevant to the function of HHAT nor would they alter the conclusions of the publication. We note that the structure of HHAT in complex with the palmitoylated Hedgehog peptide product (PDB: 7MHZ, EMDB: EMD-23837) was determined without the use of antibody 1C06. ,