The novel long non-coding RNA LATS2-AS1-001 inhibits gastric cancer progression by regulating the LATS2/YAP1 signaling pathway via binding to EZH2
Authors: Yan Xin
Dan Sun
Huan Wang
Ying Wang
Journal: Cancer Cell International (2020)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Enhancer of zeste homolog 2/EZH2; Gastric cancer; Large tumor suppressor 2/LATS2; Long non-coding RNA; Yes-associated protein 1/YAP1.
Affiliations: Laboratory of Gastrointestinal Onco-Pathology, Cancer Institute & General Surgery Institute, The First Affiliated Hospital of China Medical University, No. 155 Nanjing North Street, Heping District, Shenyang, 110001 China., Department of Oncology, Hanzhong Central Hospital, Hanzhong, 723000 China.

Abstract:  Background: To explore the expression pattern and role of the novel long non-coding RNA LATS2 antisense transcript 1 (LATS2-AS1-001) in gastric cancer (GC). Methods: qRT-PCR was applied to evaluate LATS2-AS1-001 expression and correlation with LATS2 in GC. In vitro experiments were performed to investigate the role of LATS2-AS1-001 in GC cells. RNA immunoprecipitation (RIP) was performed to assess the interaction between EZH2 and LATS2-AS1-001. LATS2/YAP1 signaling pathway proteins were detected by immunoblot. Oncomine and KMPLOT data analysis was conducted to assess the prognostic value of YAP1 in GC. Results: Decreased expression levels of LATS2-AS1-001 and LATS2 were confirmed in 357 GC tissues compared with the normal mucosa. A strong positive correlation between LATS2-AS1-001 and LATS mRNA expression was found in Pearson Correlation analysis (r = 0.719, P &lt; 0.001). Furthermore, ROC curve analysis revealed areas under the curves for LATS2-AS1-001 and LATS2 of 0.7274 and 0.6865, respectively (P &lt; 0.001), which indicated that LATS2-AS1-001 and LATS could be used as diagnostic indicators in GC. Moreover, ectopic expression of LATS2-AS1-001 decreased cell viability, induced G0/G1 phase arrest, and inhibited cell migration and invasion in GC cells. Mechanistically, overexpressing LATS2-AS1-001 upregulated LATS2 and induced YAP1 phosphorylation via binding to EZH2. Oncomine and KMPLOT database analysis demonstrated YAP1 was highly expressed in human GC samples, and high YAP1 expression predicted poor patient prognosis in GC. Conclusion: This study revealed that lncRNA LATS2-AS1-001 might serve as a potential diagnostic index in GC and act as a suppressor of GC progression. 

Retracted: No

PubPeer comments:
 Figure 4a and 4g appear to have some image issues. These are similar to another paper with overlapping author(s) discussed here: https: pubpeer.com publications B5AC11052325CD5418BD4ECB61C707 *** Contrast enhanced ![file] *** ![file] , 
 Dear Actinopolyspora Biskrensis, Thank you very much for pointing out the mistake in the figure 4a and 4g of the manuscript titled  The novel long non-coding RNA LATS2-AS1-001 inhibits gastric cancer progression by regulating the LATS2 YAP1 signaling pathway via binding to EZH2 .We solemnly committed that the cell wound-healing and Transwell assays were performed all by the first author Sun D during Dec 6, 2016 to Dec 8, 2016 and Nov 4, 2016. The images in different groups were respectively obtained and saved in different folder at 0h, 24h and 48h. To accurate record the average wound-healing and Transwell situation of scratching and invasion continuously, some overlapping images was existed in each group. In addition, the statistical results and conclusion was correct and authentic according to the corrected figures. Unfortunately, when the manuscript was prepared for publication in a hurry, so many original images of experiments were put on PPT to present the results respectively in BGC823, NC-BGC823 and AS1-BGC823 groups and SGC7901, NC-SGC7901 and AS1-SGC7901 groups. The errors were generated when the Figure 4 was assembled respectively from each group. Because lacking of labeling on each picture in the PPT resulted in figure chaos, and the assembled figures were not carefully checked according to the original record induced the repeated use the overlapping figure form one group. After the Figure 4 was assembled, which including in several figures of other assays, the Figure 4 is too small to identify these errors before publication. We deeply apologize for this careless mistake. Fortunately, however, this mistake does not affect the correctness of the results and conclusions in our article. Now, the corrected figure 4 was presented in attachment!![file] ![file] ![file] . To prove the corrected figures were the original result of the relevant experiment, the attachment demonstrated that the wound-healing figures were acquired respectively at Dec 6-8, 2016 according to 0h, 24h and 48h while the Transwell assay figures were acquired at Nov 4, 2016. I have contacted the journal and provide the original pictures to request a correction, and hope to publish a corrigendum for our paper. Thanks again for your comments! , 
 To prove the corrected figures were the original result of the relevant experiment, the attachment demonstrated that the wound-healing figures were acquired respectively at Dec 6-8, 2016 according to 0h, 24h and 48h while the Transwell assay figures were acquired at Nov 4, 2016. ![file] ![file] ![file] ![file] , 
 #3 These screenshots are too blurred to see any useful info. , 
 Retracted [14 October 2022] .  The Editors in Chief has retracted this article because of irregularities in Fig. 4 A and G. In Fig. 4 A the BGC823 and NC BGC823 panels for 0 h appear to overlap and the BGC823 and NC BGC823 panels for 24 h appear to overlap. In Fig. 4G the BGC823 and NC BGC823 panels appear to overlap and the SGC7901 and NC SGC7901 panels appear to overlap. The study had also not received sufficient ethics approval. None of the authors agree to this retraction. ,