Expression of miR-29 and STAT3 in osteosarcoma and its effect on proliferation regulation of osteosarcoma cells
Authors: Y Wang
F-Z Chen
Z-Y Wu
Y Liu
M-H Li
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: STAT3; miR-29; osteosarcoma; proliferation; apoptosis
Affiliations: Department of Orthopedics, the Fifth Hospital of Wuhan, Wuhan, Hubei, China. .

Abstract:  Objective: STAT3 has been shown to be involved in the occurrence, progression, and resistance of various tumors. The abnormal expression of miR-29 is associated with the pathogenesis of osteosarcoma. The bioinformatics analysis showed a targeting relationship between miR-29 and STAT3 3'-UTR. This study investigated whether miR-29 regulates the STAT3 expression and affects the proliferation and apoptosis of osteosarcoma cells. Patients and methods: The tumor tissues of osteosarcoma patients were collected, and the adjacent tissues were used as controls to detect the expression of miR-29 and STAT3. The Dual-Luciferase Gene Reporter Assay validated the regulatory relationship between miR-29 and STAT3. The expression of miR-29 and STAT3 in normal osteoblasts hFOB1.19, osteosarcoma SJSA-1, and MG-63 was measured. SJSA-1 cells were divided into miR-NC group and miR-29 mimic group. Cell apoptosis and proliferation were detected by flow cytometry. Results: Compared with adjacent tissues, miR-29 expression was decreased, and STAT3 expression was increased in osteosarcoma. There was a targeted regulation relationship between miR-29 and STAT3. Compared with hFOB1.19 cells, miR-29 expression in osteosarcoma SJSA-1 and MG-63 cells was decreased, with increased STAT3 expression. The transfection of miR-29 mimic significantly decreased the expression of STAT3 and p-STAT3 in SJSA-1 cells, inhibited cell proliferation, and increased cell apoptosis. Conclusions: Decreased miR-29 expression plays a role in the increase of the STAT3 expression and in the promotion of the pathogenesis of osteosarcoma. Increasing the expression of miR-29 can inhibit the proliferation of osteosarcoma cells and promote apoptosis by decreasing STAT3 expression. 

Retracted: No

PubPeer comments:
 Why would  Corresponding Author Yang Liu, MD use the e-address  dingliao8482624@126.com for academic correspondence? Did this not pique the editors interest? , 
 Left:  Figure 4. Overexpression of **miR-29** can significantly inhibit **osteosarcoma** cell proliferation and induce apoptosis. ... D, Flow cytometry to detect apoptosis in SJSA-1 cells Right:  Figure 4. Inhibition of **miR-155** expression can significantly inhibit **pancreatic cancer** cell proliferation and promote apoptosis. .. E, Flow detection of apoptosis . From [Wang, Guo Fang (2019)] . ![file] , 
 Please appreciate the effort I put into creating this Gallery of Unfortunate FACS Plots. Fig 4D [Li et al (2019)] 10.26355 eurrev_201909_18832 Fig 5D [Liu et al (2019)] 10.26355 eurrev_201911_19421 Fig 4E [Du et al (2019)] 10.26355 eurrev_201910_19278 Fig 4D [Dong et al (2019)] 10.26355 eurrev_201907_18305 ![file] Fig 4E [Wu et al (2019)] 10.26355 eurrev_201909_18852 Fig 3C [Cao et al (2019)] 10.26355 eurrev_201908_18531 Fig 4E [Wang et al (2019)] 10.26355 eurrev_201906_18181 Fig 4C [Wei et al (2019)] 10.26355 eurrev_201907_18299 ![file] , 
 These eight papers also display a strange stylised form of Western Blots, where out-of-focus geometrical ellipses hover above a featureless background. Shadowy wisps of smoke hang in the foreground but there is no real-world detail. Did I mention the absence of overlap among the different teams of authors, and their far-flung locations? Figs 3C, 4C [Li et al (2019)] 10.26355 eurrev_201909_18832 Figs 4B, 5B [Liu et al (2019)] 10.26355 eurrev_201911_19421 Figs 3C, 4C [Du et al (2019)] 10.26355 eurrev_201910_19278 Figs 3C, 4C [Dong et al (2019)] 10.26355 eurrev_201907_18305 ![file] Figs 2B, 4C [Wu et al (2019)] 10.26355 eurrev_201909_18852 Figs 2B, 3B [Cao et al (2019)] 10.26355 eurrev_201908_18531 Figs 3B, 4C [Wang et al (2019)] 10.26355 eurrev_201906_18181 Figs 3B, 4B [Wei et al (2019)] 10.26355 eurrev_201907_18299 ![file] ,