The effect of miR-30d on apoptosis and autophagy in cultured astrocytes under oxygen-glucose deprivation
Authors: Huiqing Wang
Jiao Li
Li Zhang
Fengyan Zhao
Lan Huang
Shiping Li
Yi Qu
Yu Tong
Jianghu Zhu
Dezhi Mu
Journal: Brain Research (2017)
Discipline: NEUROSCIENCE & BEHAVIOR
Keywords: Apoptosis; Astrocyte; Autophagy; Beclin1; Mir-30d
Affiliations: Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, Sichuan University, Chengdu 610041, China., Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, Sichuan University, Chengdu 610041, China; Department of Pediatrics, University of California, San Francisco, San Francisco, United States. Electronic address: .

Abstract:  MiR-30d has been demonstrated to regulate autophagy in human cancer cells. However, the role of miR-30d in astrocytes exposed to hypoxia-ischemia is not clear. In this study, we established model of oxygen and glucose deprivation (OGD) with rat primary astrocytes and evaluated the role of miR-30d in the cross-talk between autophagy and apoptosis in astrocytes after OGD. We found that miR-30d inhibited Beclin1 expression in astrocytes. Inhibition of miR-30d by antagomir significantly increased cell autophagy but decreased cell apoptosis in astrocytes exposed to OGD. Knockdown of Beclin1 reversed the upregulation of autophagy and downregulation of apoptosis induced by miR-30d inhibition in astrocytes subjected to OGD. These results strongly indicated that miR-30d played critical roles in the cross-talk between autophagy and apoptosis of astrocytes subjected to OGD by targeting Beclin1. MiR-30d is a novel target to attenuate cell injury under hypoxia-ischemia condition. 

Retracted: No

PubPeer comments:
 Figure 6C. * Green boxes: The OGD and OGD+AT+si-Beclin1 panels appear to show an overlap. The TUNEL brightness is different in both images. ![file] , 
 Dear Dr. Bik, Thank you very much for pointing out this mistake of Figurer 6 C. We have checked our original figures and found we have uploaded wrong figure by mistake. This did not affect the results and conclusion. We will contact the editor of the journal to resubmit the corrected figure. Thank you again. Sincerely, Dezhi Mu , 
 Dear Dr. Bik, Iu2019m the first author of this paper. Thank you very much for helping us to find out the mistakes. The OGD and OGD+AT+si-Beclin1 panels had overlapping area as you pointed out. I checked the original data and found that I the same site of a sample was taken twice by mistake and accidently saved in different groups. We are sorry for this mistake. We will contact the editor of the journal to submit the correct photo. , 
 I was unable to locate a correction. Could the authors provide an update? , 
 Dear Dr. Mu and Dr. Zhao, Thank you for checking the original figures. Could you please clarify how the green signal was different between these two overlapping photos? If by accident the same specimen was photographed twice, would the green signal not be more identical in the area of overlap? ,