MiR-101 affects proliferation and apoptosis of cervical cancer cells by inhibition of JAK2
Authors: H Wei
K-M Chen
W-R He
C-J Yi
X-W Wang
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: JAK2 expression; miR-101; cervical cancer; proliferation; apoptosis
Affiliations: Department of Obstetrics and Gynecology, The First Clinical Medicine College of Yangtze University, Jingzhou, Hubei, China. .

Abstract:  Objective: JAK2 expression and dysfunction play a role in tumor pathogenesis. Bioinformatics analysis revealed a targeted binding site between miR-101 and the 3'-UTR of JAK2 mRNA. This study investigated the role of miR-101 in regulating JAK2 expression and affecting the proliferation and apoptosis of cervical cancer cells. Patients and methods: The tumor tissues and adjacent tissues of patients with cervical cancer were collected. The expression of miR-101 and JAK2 was detected by qRT-PCR. The dual luciferase reporter gene assay validated the targeting relationship between miR-101 and JAK2. The cervical cancer Caski cells were cultured in vitro, and divided into miR-NC group and miR-101 mimic group. The expression of JAK2 and p-JAK2 was detected by Western blot, cell apoptosis was detected by flow cytometry, and cell proliferation was detected by EdU staining. Results: Compared with adjacent tissues, miR-101 expression was significantly decreased, and JAK2 expression was increased in cervical cancer tissues. There was a targeted regulatory relationship between miR-101 and JAK2. Compared with HcerEpic cells, miR-101 expression in HeLa and Caski was significantly decreased, and the expression of JAK2 and p-JAK2 was significantly increased. Transfection of miR-101 mimic significantly reduced the expression of JAK2 and p-JAK2 in Caski cells, reduced cell proliferation and increased cell apoptosis. Conclusions: The decrease of miR-101 expression and the increase of JAK2 expression play a role in cervical cancer, while the increase of miR-101 expression can inhibit the proliferation and promote the apoptosis of cells by inhibiting the expression of JAK2. 

Retracted: Yes

PubPeer comments:
 Please appreciate the effort I put into creating this Gallery of Unfortunate FACS Plots. Fig 4D [Li et al (2019)] 10.26355 eurrev_201909_18832 Fig 5D [Liu et al (2019)] 10.26355 eurrev_201911_19421 Fig 4E [Du et al (2019)] 10.26355 eurrev_201910_19278 Fig 4D [Dong et al (2019)] 10.26355 eurrev_201907_18305 ![file] Fig 4E [Wu et al (2019)] 10.26355 eurrev_201909_18852 Fig 3C [Cao et al (2019)] 10.26355 eurrev_201908_18531 Fig 4E [Wang et al (2019)] 10.26355 eurrev_201906_18181 Fig 4C Wei et al (2019) 10.26355 eurrev_201907_18299 ![file] , 
 #1 Astonishing. One is left with a concern that someone associated with the journal is involved. Very impressive effort Hoya. , 
 These eight papers also display a strange stylised form of Western Blots, where out-of-focus geometrical ellipses hover above a featureless background. Shadowy wisps of smoke hang in the foreground but there is no real-world detail. Did I mention the absence of overlap among the different teams of authors, and their far-flung locations? Figs 3C, 4C [Li et al (2019)] 10.26355 eurrev_201909_18832 Figs 4B, 5B [Liu et al (2019)] 10.26355 eurrev_201911_19421 Figs 3C, 4C [Du et al (2019)] 10.26355 eurrev_201910_19278 Figs 3C, 4C [Dong et al (2019)] 10.26355 eurrev_201907_18305 ![file] Figs 2B, 4C [Wu et al (2019)] 10.26355 eurrev_201909_18852 Figs 2B, 3B [Cao et al (2019)] 10.26355 eurrev_201908_18531 Figs 3B, 4C [Wang et al (2019)] 10.26355 eurrev_201906_18181 Figs 3B, 4B [Wei et al (2019)] 10.26355 eurrev_201907_18299 ![file] , 
 It is also worth comparing 4(D)  flow detection of cell proliferation (below) with (above) Fig 4(E)  EdU staining for cell proliferation from [Dong et al (2019)] . ![file] , 
 Retracted [April 2021] .  Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cMiR-101 affects proliferation and apoptosis of cervical cancer cells by inhibition of JAK2, by H. Wei, W.-R. He, K.-M. Chen, X.-W. Wang, C.-J. Yi, published in Eur Rev Med Pharmacol Sci 2019; 23 (13): 5640-5647u2013DOI: 10.26355 eurrev_201907_18299u2013PMID: 31298355u201d has been withdrawn.  The Publisher apologizes for any inconvenience this may cause. , 
 http: www.most.gov.cn zxgz kycxjs kycxgzdt 202201 t20220127_179206.html Google Translated Date: January 27, 2022 14:44 Source: Ministry of Science and Technology u3000u300039. The paper  miR-101 affects proliferation and apoptosis of cervical cancer cells by inhibition of JAK2 by Wei Hua from Yangtze University, the corresponding author and the first author. After investigation, there are acts of tampering with data and charts. Wei Hua will be dealt with as follows: the dissertation s qualification for achievement grade recognition in the school will be cancelled, the dissertation shall not be used for award application, year-end assessment, employment period assessment, professional title evaluation, etc., and the application qualification for scientific research projects will be cancelled for 3 years. u3000u3000(Notification link: http: kxyj.yangtzeu.edu.cn info 1043 4457.htm) ,