Knockdown of long non-coding RNA PEG10 inhibits growth, migration and invasion of gastric carcinoma cells via up-regulating miR-3200
Authors: D F Kong
J Wang
X Q Chu
D Zhang
Journal: Neoplasma (2018)
Discipline: CLINICAL MEDICINE
Keywords: Gastric cancer; PEG10; miR-3200; AEG1; LncRNA
Affiliations: Department of Gastrointestinal Surgery, Jining No. 1 People's Hospital, Jining, China.

Abstract:  Gastric cancer is the main leading cause of cancer-related death worldwide. The aberrant expression of paternally expressed gene 10 (PEG10) is involved in development of a range of cancers. However, the potential biological function and the underling mechanism of PEG10 in human gastric carcinoma are still unknown. Knocking down LncRNA PEG10 might represent a promising therapeutic strategy for the treatment of gastric cancer. The expression of PEG10, miR-3200, and AEG1 in human gastric carcinoma NCI-N87 cells were altered by cell transfection assay. Cell viability, migration, invasion, and apoptosis were determined by trypan blue exclusion, Transwell assay, and flow cytometric analysis, respectively. RNA and protein expression level of gene was analyzed by real-time PCR and Western blot. Luciferase reporter assay was conducted to determine the target gene of miR-3200. JNK and Wnt signal pathway protein expressions were tested by Western blot. The up-regulation of PEG10 was found in clinical samples. PEG10 knockdown effectively inhibited gastric carcinoma cell viability, migration, and invasion, but promoted cell apoptosis. This tumor-suppressing effect of PEG10 knockdown might be realized by up-regulating miR-3200 in vitro and in vivo. AEG1 was a direct target gene of miR-3200. Moreover, miR-3200 might suppress NCI-N87 cells by negative regulating AEG1. Up-regulating miR-3200 effectively blocked JNK and Wnt pathways likely via down-regulating AEG1. PEG10 knockdown played a carcinostatic role via up-regulating miR-3200 and further regulating AEG1 in gastric carcinoma cells, during which process, JNK pathway and Wnt pathway were blocked. 

Retracted: No

PubPeer comments:
 This paper belongs to a set of over 400 papers (as per February 2020) that share very similar Western blots with tadpole-like shaped bands, the same background pattern, and striking similarities in title structures, paper layout, bar graph design, and - in a subset - flow cytometry panels. Despite these similarities, these papers are authored by researchers from different departments and institutes, with almost no overlap in authors. The list of these papers is available here: https: docs.google.com spreadsheets d 1KXqTAyl4j-jVorFPMD2XRpr76LcIKJ0CVyIvRj0exYQ edit?usp=sharing A more detailed description of this set of papers, suspected to all be generated by a common source, a paper mill , can be found here: * https: forbetterscience.com 2020 01 24 the-full-service-paper-mill-and-its-chinese-customers (January 2020) * https: scienceintegritydigest.com 2020 02 21 the-tadpole-paper-mill (February 2020) The same paper mill has been described in these papers: * Jana Christopher - Systematic fabrication of scientific images revealed - FEBS Letters (2018) - https: febs.onlinelibrary.wiley.com doi full 10.1002 1873-3468.13201 * Jennifer A Byrne and Jana Christopher - Digital magic, or the dark arts of the 21st centuryu2014how can journals and peer reviewers detect manuscripts and publications from paper mills? - FEBS Letters (2020) - https: febs.onlinelibrary.wiley.com doi full 10.1002 1873-3468.13747 , 
 [Source: Jining First People s Hospital Release time: 2021-12-17] : In order to strengthen the construction of scientific research integrity, severely punish violations, and create a good environment for scientific and technological innovation, the relevant paper issues recently investigated and dealt with by this unit are hereby notified as follows. 7. The paper  Knockdown of long non-coding RNA PEG10 inhibits growth, migration and invasion of gastric carcinoma cells via up-regulating miR-3200 , published by Kong Dongfang as the corresponding author and Wang Jing as the first author, was investigated. There are academic misconducts such as tampering with data, fabricating research processes, and improperly signing names. The relevant responsible persons are dealt with as follows: Kong Dongfang (corresponding author, combined with another paper): Cancellation of application qualifications for scientific research projects, scientific research awards, scientific and technological achievements, scientific and technological talent plans within 6 years; cancellation of 6 years as a nominee or recommender , nominees or recommenders, review experts and other qualifications; cancel their membership or membership in academic institutions such as societies, associations, etc.; recover the author s personal research awards and honorary titles; postpone promotion to a higher level for 2 years Professional and technical positions; instructed to contact the magazine as soon as possible to withdraw the manuscript; administrative warning. Wang Jing (first author, merged with 2 other papers): Cancellation of application qualifications for scientific research projects, scientific research awards, scientific and technological achievements, scientific and technological talent plans within 6 years; cancellation of 6 years as a nominee or recommender, nominee or recommender, Review experts and other qualifications; cancel their membership or membership in academic institutions such as societies, associations, etc.; recover the author s personal research awards and honorary titles; revoke the higher-level professional and technical positions that have been obtained; postpone promotion 4 Senior professional and technical position; administrative warning. ,