Long non-coding RNA XIST regulates gastric cancer progression by acting as a molecular sponge of miR-101 to modulate EZH2 expression
Authors: Le-Zong Chen
Rui-Hua Xu
Da-Zhi Xu
Helene Pelicano
Yun-Xin Lu
Dong-Sheng Zhang
Miao-Zhen Qiu
Dong-Liang Chen
Zhao-Lei Zeng
Peng Huang
Dan Xie
Hui-Yan Luo
Huai-Qiang Ju
Yu-Hong Li
Feng Wang
Feng-Hua Wang
De-Shen Wang
Zhi-Wei Zhou
Journal: Journal of Experimental & Clinical Cancer Research (2016)
Discipline: CLINICAL MEDICINE
Keywords: EZH2; Gastric cancer; Long non-coding RNA; lncRNA XIST; miR-101.
Affiliations: State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Department of Medical Oncology, Sun Yat-sen University Cancer Center, No. 651 Dong Feng East Road, Guangzhou, 510060, China., University of Texas M.D. Anderson Cancer Center, Houston, TX, USA., State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Department of Medical Oncology, Sun Yat-sen University Cancer Center, No. 651 Dong Feng East Road, Guangzhou, 510060, China. .

Abstract:  Background: Long non-coding RNAs (lncRNAs) have emerged as critical regulators of tumor progression. However, the role and molecular mechanism of lncRNA XIST in gastric cancer is still unknown. Methods: Real-time PCR analysis was performed to measure the expression levels of lncRNA XIST in gastric cancer tissues and cell lines, the correlation between lncRNA XIST expression and clinicopathological characteristics and prognosis was analyzed in gastric cancer patients. The biological function of lncRNA XIST on gastric cancer cells were determined both in vitro and in vivo. The regulating relationship between lncRNA XIST and miR-101 was investigated in gastric cancer cells. Results: lncRNA XIST was significantly up-regulated in gastric cancer tissues and cell lines. Overexpression of lncRNA XIST was markedly associated with larger tumor size, lymph node invasion, distant metastasis and TNM stage in gastric cancer patients. Functionally, knockdown of lncRNA XIST exerted tumor-suppressive effects by inhibiting cell proliferation, migration and invasion in vitro and tumor growth and metastasis in vivo. Furthermore, an inverse relationship between lncRNA XIST and miR-101 was found. Polycomb group protein enhancer of zeste homolog 2 (EZH2), a direct target of miR-101, could mediated the biological effects that lncRNA XIST exerted. Conclusions: lncRNA XIST is up-regulated and is associated with aggressive tumor phenotypes and patient survival in gastric cancer, and the newly identified lncRNA XIST/miR-101/EZH2 axis could be a potential biomarkers or therapeutic targets for gastric cancer patients. 

Retracted: No

PubPeer comments:
 In Figure 6c a micrograph has been duplicated and presented as something else. ![file] , 
 #1 Dear Dr. Indigofera Tanganyikensis, I am Rui-hua Xu, the corresponding author of the article. Thank you very much for your comment about our article, and we apologize for this mistake, and for any inconvenience caused by this. We carefully checked the manuscript and the raw data immediately upon receiving your comment, we found that the mentioned image has indeed been mismatched during the preparation of Fig. 6c. We will contact the journal office to issue a correction. Thank you very much again for your comment! Yours sincerely! , 
 Dear authors, I am curious about the author contributions. In the notice of Authorsu2019 contributions: QMZ and WDS supported the experiments and helped to draft the manuscript. However, no authors in the author list match QMZ. Who is QMZ and what about the exact role of QMZ in the present work? ,