Protective functions of myricetin in LPS-induced cardiomyocytes H9c2 cells injury by regulation of MALAT1
Authors: Jianhui Sun
Xuezhong Zhou
Jinliang Sun
Journal: European journal of medical research (2019)
Discipline: CLINICAL MEDICINE
Keywords: Anti-inflammation; MALAT1; Myricetin; NF-κB.
Affiliations: Department of Cardiology, The First People's Hospital of Changzhou, No. 185 Juqian Street, Changzhou, 213000, China. ., Department of Cardiology, The First People's Hospital of Changzhou, No. 185 Juqian Street, Changzhou, 213000, China.

Abstract:  Background: Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a crucial mediator in response to inflammation. Myricetin protects cardiomyocytes against inflammatory injury. However, it's still unexplored whether myricetin exerted anti-inflammatory properties via MALAT1. The purpose of our study was to validate the cardio-protective function of myricetin against myocarditis and its underlying mechanism in vitro. Methods: H9c2 cells were pre-incubated with myricetin before stimulation with lipopolysaccharide (LPS). Enforced silence of MALAT1 was achieved by transducing short hairpin (sh)-MALAT1 into H9c2 cells. Next, cell viability and apoptotic cells were detected with cell counting kit-8 (CCK-8) and Annexin V-fluorescein isothiocyanate/propidium iodide (Annexin V-FITC/PI) apoptosis detection kit, respectively. Western blot assay was conducted to examine apoptosis-relative proteins, pro-inflammatory factors, and signaling regulators. Quantitative real-time PCR (qRT-PCR) was performed to quantify pro-inflammatory factors and MALAT1 at mRNA levels. Enzyme-linked immune sorbent assay (ELISA) was employed to determine protein concentration of pro-inflammatory factors. Results: Myricetin ameliorated LPS-elicited reduction of cell viability, augment of apoptosis, and overexpression of monocyte chemo-attractant protein-1 (MCP-1) and interleukin-6 (IL-6) in H9c2 cells. Meanwhile, phosphorylation of p65 and inhibitor of nuclear factor kappa B alpha (IκBα) were suppressed. Besides, myricetin enhanced the expression of MALAT1 which was originally down-regulated by LPS. However, the protective effects of myricetin against LPS-caused inflammatory lesions were abrogated in MALAT1-deficiency cells, with the restored phosphorylation of p65 and IκBα. Conclusion: Myricetin possessed an anti-inflammatory function against LPS-induced lesions in cardiomyocytes. Mechanically, myricetin up-regulated MALAT1, blocked LPS-evoked activation of nuclear factor-κB (NF-κB) inflammatory pathway, and, finally, exerted cardio-protective effects. 

Retracted: No

PubPeer comments:
 The Western blots and other figures in this paper have an interesting layout that appears to be shared with many other papers, as discussed in a January 2020 post on Leonid Schneider s blog: https: forbetterscience.com 2020 01 24 the-full-service-paper-mill-and-its-chinese-customers The list of these papers is available here: https: docs.google.com spreadsheets d 1KXqTAyl4j-jVorFPMD2XRpr76LcIKJ0CVyIvRj0exYQ edit?usp=sharing In almost all papers, Western blot panels within the same figure, and across figures and papers, appear to share the same background, while the bands are regularly spaced, all have similar rounded edges without the usual smudges and specks, and with some bands showing a recognizable  jumping sardine shape. Also in almost all papers, bar graphs show a characteristic layout with bars in solid shades of grey, and the left-most bar in black, as well as double-edged error bars. Many papers have  cell viability and  apoptotic cells graphs. For example, here is Figure 3 from this paper. ![file] , 
 Here are two panels from Figure 7 from this paper, enhanced to bring out the background. Note the similar background across the different panels, with e.g. small vertical stripes above the left edges of the first and second bands from the left. ![file] , 
 #2 An August 19, 2021 [announcement ](http: www.nhc.gov.cn cms-search xxgk getManuscriptXxgk.htm?id=159bcc53148340d48336060e65571a1c) by  National Health Commission of the People s Republic of China reported the results of investigation of 10 papers flagged on Pubpeer, together with disciplinary actions against the authors. The current paper is the #5 case in the listand the investigation concluded that this paper was purchased from a papermill. Here is a screenshot: ![file] , 
 #3 Google Translation: -------------------------------- 5. Sun Jinliang, Changzhou First People s Hospital, Jiangsu Province, as the first author and corresponding author, published the paper  Protective functions of myricetin in LPS-induced cardiomyocytes H9c2 cells injury by regulation of MALAT1 in the European Journal Of Medical Research. After investigation, It is a serious academic misconduct to write and invest on behalf of a third party. Changzhou First People s Hospital give Sun Jinliang comrades following process: party a serious warning; the withdrawal of the above papers published; cancel the declaration of eligibility at all levels of research and technological achievements five years; suspended incentive pay for performance Shier months; Cancellation of a senior appointment. (Notification link: http: www.czfph.com kyjs ShowInfo.asp?InfoID=9672) ------------------------ Can the journal editors confirm that they have received the authors request to withdraw the paper? , 
 So far cited 16 times with no end in sight. ![file] ,