Transcriptional hierarchy of Aeromonas hydrophila polar-flagellum genes
Authors: Jonathan G Shaw
Raquel Molero
Susana Merino
Juan M Tomás
Markus Wilhelms
Journal: Journal of Bacteriology (2011)
Discipline: MICROBIOLOGY
Keywords: Aeromonas hydrophila; polar-flagellum; transcription; σ70 dependent; LafK protein
Affiliations: Departamento Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain., Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain, Division of Genomic Medicine, School of Medicine and Biomedical Sciences, University of Sheffield, Sheffield S10 2RX, United Kingdom

Abstract:  Aeromonas hydrophila polar-flagellum class I gene transcription is σ70 dependent, which is consistent with the fact that the A. hydrophila polar flagellum is constitutively expressed. In contrast to other bacteria with dual flagellar systems such as Vibrio parahaemolyticus, the A. hydrophila LafK protein does not compensate for the lack of the polar-flagellum regulator FlrA (V. parahaemolyticus FlaK homologue). This is consistent with the fact that the A. hydrophila FlrA mutation abolishes polar-flagellum formation in liquid and on solid surfaces but does not affect inducible lateral-flagellum formation. The results highlight that the polar- and lateral-flagellum interconnections and control networks are specific and that there are differences between the dual flagellar systems in A. hydrophila and V. parahaemolyticus. Furthermore, our results indicate that the A. hydrophila polar-flagellum transcriptional hierarchy (also in class II, III, and IV genes) shares some similarities with but has many important differences from the transcriptional hierarchies of Vibrio cholerae and Pseudomonas aeruginosa. The A. hydrophila flhF and flhG genes are essential for the assembly of a functional polar flagellum because in-frame mutants fail to swim in liquid medium and lack the polar flagellum. In Vibrio and Pseudomonas flhG disruption increases the number of polar flagella per cell, and Pseudomonas flhF disruption gives an aberrant placement of flagellum. Here, we propose the gene transcriptional hierarchy for the A. hydrophila polar flagellum. 

Retracted: No

PubPeer comments:
 *[right]* Fig 2A of [Li et al (2020)] .  miR-7 induces apoptosis in TPC-1 cancer cells. A. Colony formation by TPC-1 cells transfected with miRNC and miR-7 mimics . Plus 180-deg-flipped version. *[left]* Figure 2A of [Hou et al (2020)] .  Clonogenic assay for determination of anticolony potential of Cinnamolide in human cervical HeLa cancer cells. Cinnamolide treatment led to a significant and dose-dependent reduction in the number of cancer cell colonies . ![file] Two panels are repeated. , 
 ![file] Thankyou for pointing it out. We checked our data and found our data correct. I am attaching a snapshot of the lab note book of my student to clear the doubt. We can also upload the high resolutions images in triplicate, if needed , 
 #2 Sadly, J.B.U.On - where Hou et al (2020) was published - appears to be rampant with pirated and manipulated Figures. , 
 According to the dates published on the articles Hou et al was submitted a few days (26 09 2019) before Li et al (Received 2019 Oct 4). , 
 Another sighting! Fig 3E from [Liu, Liu Zhao (2020)] .  Colony formation of the NC or Si-MAPK1 transfected DoTc2 cells . ![]  Received 2019 Jan 4; Accepted 2019 Feb 2. , 
 This study was designed, conducted, written, and published by Xiaobin Li, who asked me to be his correspondence for publishing. It is my negligence that this published material was not thoroughly reviewed before submission. I have instructed him to take full responsibility for this matter and actively engage in subsequent processing. This article will be withdrawn as soon as possible and a full investigation will be held, as neither me or our institution allows such misconduct. We will make sure to strengthen our management and educate our students. We sincerely thank you for putting this issue to our attention; your thoughts are of great value for us. We hope that this issue can be satisfactorily resolved. , 
 *[right]* Figure 3C from [Li et al (2020)] .  miR-7 reduces the migration and invasion of cancer cells. ... C. Transwell assay of TPC-1 cancer cells transfected with miR-NC and miR-7 mimics. *[left]* Figure 7 from [Meng, Tang Qiu (2019)] .  Inhibition of Saos-2 cell invasion by Lactucopicrin at indicated concentrations as depicted by transwell assay. Lactucopicrin led to dose-dependent inhibition of cell invasion of Saos-2 osteosarcoma cells. ![file] , 
 #6 Dr. Liu: Thank you for responding to these concerns. If the study was u201cdesigned, conducted, written, and published by Xiaobin Li,u201d what was the contribution of the other 8 authors (including yourself)? On a related note, the 2nd author was listed as an u201cEqual contributoru201d with the first author. Was that incorrect given that Dr. Li is being identified as the sole responsible party? You may also wish to review this thread: https: pubpeer.com publications 99FEEDAD098A6FFBC4264E2B781CBC , 
 *[right]* Figure 2D.  miR-7 induces apoptosis in TPC-1 cancer cells.... D. AO EB staining of cancer cells transfected with miR-NC and miR-7 mimics. *[left]* Figure 2 from [Xiao et al (2020)] .  AO EB staining showing induction of apoptosis of SCC-1 cancer cells treated with Voacangine in a dose-dependent manner . Replacing an earlier, problematic image. ![file] None of this really inspires faith in the integrity of the papers, or the editorial process. , 
 I am responsible for this project and the manuscript, and I will be responsible for all subsequent problems associated with this study. Thank you very much for your serious works and pointing this questions. Currently, I am in the process of contacting the editorial office for further discussions regarding withdrawal and correction of this manuscript. Sincerely yours, xiaobin Li , 
 *[left]* Figure 1C.  miR-7 inhibits the proliferation of papillary cancer cells... (C) absorbance of TPC-1 cancer cells at 570 nm transfected with miR-NC and miR-7 mimics for different time intervals.  *[right]* Figure 1C from [Liang et al (2020)] .  miR-466 is downregulated in human lung cancer and its overexpression declines the cancer cell proliferation. ... C: Assessment of proliferation of A549 cancer cells transfected with miR-NC or miR-466 mimics. ![file] Can the authors comment on the identical results from otherwise-unrelated research? , 
 #10 Is there any progress with these discussions? , 
 #12 Thank you very much to point out these problems. I have contacted the editorial office to withdraw the manuscript and explain these problems. , 
 Retracted [15 July 2021] .  In this article, part of the experiment was conducted and the results were provided by a third-party biological laboratory due to the lack of necessary instruments and reagents. However, we later found that the wrong results were provided to us by the third-party laboratory after completing the experiment, and we have included part of those mistaken data in the article. Although the original data were later provided, we recognize that the results and conclusions obtained based on these data may be unreliable. After careful discussion, all participating authors have agreed that, based on the need of scientific accuracy and honesty, it is necessary to withdraw the article. We are deeply sorry for the inconvenience this may have caused, and we hope your journal editorial office would accept our sincere apologies and application to withdraw the article. ,