Knockdown of Long Noncoding RNA CAT104 Inhibits the Proliferation, Migration, and Invasion of Human Osteosarcoma Cells by Regulating MicroRNA-381
Authors: Baoyin Du
Yuanjie Tan
Li Feng
Lei Wang
Baofang Tian
Bo Xia
Journal: Oncology research (2018)
Discipline: CLINICAL MEDICINE
Keywords: Osteosarcoma; long noncoding RNA CAT104; microRNA-381; cell proliferation; migration
Affiliations: Department of Emergency Trauma Surgery, Jining No. 1 People's Hospital, Jining, Shandong, P.R. China., Second Department of Orthopedics, The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, P.R. China., Department of Cardiology, Weihai Hospital of Traditional Chinese Medicine, Weihai, Shandong, P.R. China.

Abstract:  Osteosarcoma is the most common primary malignant bone tumor in children and adolescents. This study aimed to explore the effects of long noncoding RNA CAT104 and microRNA-381 (miR-381) on osteosarcoma cell proliferation, migration, invasion, and apoptosis, as well as the underlying potential mechanism. We found that CAT104 was highly expressed in osteosarcoma MG63 and OS-732 cells. Knockdown of CAT104 significantly inhibited OS-732 cell proliferation, migration, and invasion, but promoted cell apoptosis. CAT104 regulated the expression of miR-381, and miR-381 participated in the effects of CAT104 on OS-732 cells. Zinc finger E-box-binding homeobox 1 (ZEB1) was a direct target gene of miR-381, which was involved in the regulatory roles of miR-381 in OS-732 cell proliferation, migration, invasion, and apoptosis, as well as c-Jun N-terminal kinase (JNK) and Wnt/β-catenin pathways. In conclusion, our research verified that suppression of CAT104 exerted significant inhibitory effects on osteosarcoma cell proliferation, migration, and invasion by regulating the expression of miR-381 and downstream ZEB1, as well as JNK and Wnt/β-catenin pathways. 

Retracted: No

PubPeer comments:
 This paper belongs to a set of over 400 papers (as per February 2020) that share very similar Western blots with tadpole-like shaped bands, the same background pattern, and striking similarities in title structures, paper layout, bar graph design, and - in a subset - flow cytometry panels. Despite these similarities, these papers are authored by researchers from different departments and institutes, with almost no overlap in authors. The list of these papers is available here: https: docs.google.com spreadsheets d 1KXqTAyl4j-jVorFPMD2XRpr76LcIKJ0CVyIvRj0exYQ edit?usp=sharing A more detailed description of this set of papers, suspected to all be generated by a common source, a paper mill , can be found here: * https: forbetterscience.com 2020 01 24 the-full-service-paper-mill-and-its-chinese-customers (January 2020) * https: scienceintegritydigest.com 2020 02 21 the-tadpole-paper-mill (February 2020) The same paper mill has been described in these papers: * Jana Christopher - Systematic fabrication of scientific images revealed - FEBS Letters (2018) - https: febs.onlinelibrary.wiley.com doi full 10.1002 1873-3468.13201 * Jennifer A Byrne and Jana Christopher - Digital magic, or the dark arts of the 21st centuryu2014how can journals and peer reviewers detect manuscripts and publications from paper mills? - FEBS Letters (2020) - https: febs.onlinelibrary.wiley.com doi full 10.1002 1873-3468.13747 , 
 [Source: Jining First People s Hospital Release time: 2021-12-17] : In order to strengthen the construction of scientific research integrity, severely punish violations, and create a good environment for scientific and technological innovation, the relevant paper issues recently investigated and dealt with by this unit are hereby notified as follows. 21. The paper  Knockdown of Long Noncoding RNA CAT104 Inhibits the Proliferation, Migration, and Invasion of Human Osteosarcoma Cells by Regulating MicroRNA-381 published by Du Baoyin as the corresponding author and Xia Bo as the first author, was found to be tampered with Academic Misconduct in Data. Our hospital will deal with the relevant responsible persons as follows: Du Baoyin (corresponding author): cancel the application qualifications for scientific research projects, scientific research awards, scientific and technological achievements, scientific and technological talent plans within 5 years; cancel the 5-year nomination or recommender, nominated or recommender, Review experts and other qualifications; cancel their membership or membership in academic institutions such as societies and associations; postpone promotion to a higher-level professional and technical position for one year; order to contact the magazine as soon as possible to withdraw the manuscript; administrative warning. Xia Bo (first author): Cancellation of application qualifications for scientific research projects, scientific research awards, scientific and technological achievements, and scientific and technological talent plans within 5 years; cancellation of 5-year qualifications as nominees or recommenders, nominees or recommenders, review experts, etc.; postponement of promotion 1 A senior professional and technical position; cancel the project committee or membership of academic institutions such as societies, associations, etc. that he has obtained; recover the matching funds for scientific research projects; warning from the party. Feng Li and Tian Baofang (participating authors): Talks about scientific research integrity; Weihai Traditional Chinese Medicine Hospital treated Tan Yuanjie (participating author) as follows: Criticism and education. ,