miR-182-5p improves the viability, mitosis, migration, and invasion ability of human gastric cancer cells by down-regulating RAB27A
Authors: Shengqiang Yu
Sen Xu
Yongwei Li
Zhengfei Shan
Liyan Bi
Shudong Chen
Yuling Li
Journal: Bioscience Reports (2017)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: RAB27A; gastric cancer; miR-182-5p.
Affiliations: Department of Pathophysiology, Binzhou Medical University, Yantai 264003, Shandong, China., Department of Gastrointestinal Surgery Ward II, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, Shandong, China., Department of Urology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, Shandong, China., Department of Transformative Otology and Neuroscience Center, Binzhou Medical University, Yantai 264003, Shandong, China., Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai 264003, Shandong, China .

Abstract:  We investigated the effect of miR-182-5p on the viability, proliferation, invasion, and migration ability of human gastric cells by regulating the expression of RAB27A. Real-time PCR assay was used to detect the expression of miR-182-5 and RAB27A in human gastric carcinoma tissues, para-carcinoma tissues, and different cell lines. Western blotting was also used to determine the RAB27A expression in both tissues and cell lines. We chose the HGC-27 cell line as experiment subject as it demonstrated the highest miR-182-5p level. HGC-27 cells were transfected with different vectors and the cell viability, mitosis, invasion, and migration ability were measured through MTT assay, flow cytometry (FCM) analysis, Transwell assay, and wound healing assay. In comparison with the normal tissues, miR-182-5p is expressed at a higher level in gastric cancer (GC) tissues, while RAB27A is expressed at a lower level in cancerous tissues. The down-regulation of miR-182-5p and up-regulation of RAB27A can significantly decrease the viability, migration, invasion, and mitosis of HGC-27 cells. The target relationship between miR-182-5p and RAb27A was confirmed through a dual-luciferase reporter gene assay and Western blot assay. miR-182-5p enhances the viability, mitosis, migration, and invasion of human GC cells by down-regulating RAB27A. 

Retracted: Yes

PubPeer comments:
 Figure 4A appears to have two same images Also, the email address is strange, which does not correspond with his her name (Correspondence: SenXu, dashashan1000@163.com) ![file] , 
 Retracted [22 September 2021] . ------------------------ This article is being retracted from *Bioscience Reports* at the request of the Editor-in-Chief and the Editorial Board following receipt of a notification from a reader, alerting the Editorial Board to a duplicated panel in Figure 4A. Post publication review by the Editorial Office also identified signs of image manipulation in the original version of Figure 4A as well as further duplications in the original version of 4C, which were replaced by the authors during the revision process. The authors have been contacted in regards to the Retraction, and have not responded to the Journal s queries and the concerns raised. Given the extent of the issues raised, the Editorial Board stand by the decision to retract the article. ,