Seabuckthorn Pulp Oil Protects against Myocardial Ischemia–Reperfusion Injury in Rats through Activation of Akt/eNOS
Authors: Dharamvir S Arya
Nanda Gamad
Rajiv Kumar Malhotra
Sameer Goyal
Salma Malik
Kapil Suchal
Jagriti Bhatia
Shreesh Ojha
Tapas C Nag
Journal: Frontiers in Pharmacology (2016)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: apoptosis; edible oil; inflammation; lehberry; myocardial ischemia–reperfusion injury
Affiliations: Cardiovascular Research Laboratory, Department of Pharmacology, All India Institute of Medical Sciences New Delhi, India., Department of Pharmacology, R. C. Patel Institute of Pharmaceutical Education and Research Shirpur, India., Department of Anatomy, All India Institute of Medical Sciences New Delhi, India., Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, United Arab Emirates University Abu Dhabi, UAE.

Abstract:  Seabuckthorn (SBT) pulp oil obtained from the fruits of seabuckthorn [Hippophae rhamnoides L. (Elaeagnaceae)] has been used traditionally for its medicinal and nutritional properties. However, its role in ischemia-reperfusion (IR) injury of myocardium in rats has not been elucidated so far. The present study reports the cardioprotective effect of SBT pulp oil in IR-induced model of myocardial infarction in rats and underlying mechanism mediating activation of Akt/eNOS signaling pathway. Male albino Wistar rats were orally administered SBT pulp oil (5, 10, and 20 ml/kg/day) or saline for 30 days. On the day 31, ischemia was induced by one-stage ligation of left anterior descending coronary artery for 45 min followed by reperfusion for 60 min. SBT pulp oil pretreatment at the dose of 20 ml/kg observed to stabilize cardiac function and myocardial antioxidants such as glutathione, superoxide dismutase, catalase, and inhibited lipid peroxidation evidenced by reduced malondialdehyde levels as compared to IR-control group. SBT pulp oil also improved hemodynamic and contractile function and decreased tumor necrosis factor and activities of myocyte injury marker enzymes; lactate dehydrogenase and creatine kinase-MB. Additionally, a remarkable rise in expression of pAkt-eNOS, Bcl-2 and decline in expression of IKKβ/NF-κB and Bax was observed in the myocardium. The histopathological and ultrastructural salvage of cardiomyocytes further supports the cardioprotective effect of SBT pulp oil. Based on findings, it can be concluded that SBT pulp oil protects against myocardial IR injury mediating favorable modulation of Akt-eNOS and IKKβ/NF-κB expression. 

Retracted: No

PubPeer comments:
 Image duplication in panels A and F of Fig 5. ![file] , 
 #1  The effect of SBT pulp oil on myocardium ultrastructure (scale bar: 500 nm). (A) Sham; (B) Ischemia-reperfusion control; **(C) Seabuckthorn pulp oil 5 ml kg day + ischemia-reperfusion; (D) Seabuckthorn pulp oil 10 ml kg day + ischemia-reperfusion;** (E) Seabuckthorn pulp oil 20 ml kg day + ischemia-reperfusion; (F) Seabuckthorn pulp oil 20 ml kg day per se; (C) and (D) also overlap. ![file] , 
 In **Fig. 6**, the beta-actin loading controls are **all** identical (4 panels in https: www.frontiersin.org files Articles 186896 fphar-07-00155-HTML-r1 image_m fphar-07-00155-g006.jpg ) see also https: pubpeer.com publications 85CE765422A7061492D1A144A2D78C https: pubpeer.com publications A8C0F28D2602FAB209DF9E79E81674 https: pubpeer.com publications E5FF53F96CC9076AA8AA86A3570867 = will there be new corrections ? https: www.ncbi.nlm.nih.gov pubmed 30766488 ,