Long non-coding RNA LINC00628 suppresses the growth and metastasis and promotes cell apoptosis in breast cancer
Authors: X-D He
X-D Zheng
W-Q Nian
X-Y Liu
D-Q Chen
Y Cao
X-H Zeng
Journal: European review for medical and pharmacological sciences (2017)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: Breast cancer; Long non-coding RNA; LINC00628; Tumorigenesis; Prognosis
Affiliations: Department of Clinical Laboratory, Chongqing Cancer Institute/Hospital, Chongqing, China. .

Abstract:  Objective: Breast cancer is the most common malignant tumor in women. However, the detailed mechanisms of its tumorigenesis remain largely unknown. Evidence and data have shown that abnormality in expression of Long non-coding RNA (LncRNA) is closely related to tumorigenesis. The aim of this study is to identify the detailed mechanisms of LncRNA LINC00628 in breast cancer. Patients and methods: The expression of LINC00628 in breast cancer tissues, adjacent non-cancerous tissues and cell lines were detected by qRT-PCR. Kaplan-Meier method and log-rank test were applied to analyze the overall survival of patients with low and high expression level of LINC00628 respectively. The LCC2 and MCF-7 cells were transfected with LINC00628 and the proliferation, invasion and migration were examined. The cell cycle distribution and cell apoptosis rate in LCC2 and MCF-7 cells after transfection with LINC00628 were explored by flow cytometry. The relative expression level of Bcl-2, Bax and Caspase-3 protein in LCC2 and MCF-7 cells after transfection with LINC00628 was detected by Western blotting. Results: The relative expression level of LINC00628 in breast cancer tissues and cell lines were significantly decreased and the low expression level of LINC00628 has a poorer prognosis and lower overall survival rate. The overexpression of LINC00628 suppressed breast cancer cells proliferation, invasion and migration. Further, with the overexpression of LINC00628, cell cycle was arrested in G0/G1 phase in breast cancer cells and cell apoptosis was promoted. The relative expression of Caspase-3 and Bax protein were significantly increased and the relative expression of Bcl-2 protein was significantly decreased after transfection with LINC00628. Conclusions: The expression of LINC00628 was decreased in breast cancer. The overexpression of LINC00628 suppressed the proliferation, invasion and migration of breast cancer cells and promoted cell apoptosis associated with the regulation of Bcl-2/Bax/Caspase-3 signal pathway. 

Retracted: No

PubPeer comments:
 Here comparing *[right]* [Chen et al (2017)] with *[left]* [He et al (2018)] . The textual similarity raises the possibility that both are the work of unknown authors at a papermill. ![file] It is not a case of two teams of authors independently creating similar summaries of the literature, as the citation lists are different (refs 8 to 16, and 11 to 23) and in both case the citations are unrelated to the statements that they are claimed to support. Comparing Figs 2B *[right]* and 1C *[left]*. While it is not impossible that 60 cases of breast cancer and 80 cases of osteosarcoma would have similar 36-month post-surgery survival curves, what are the odds of two teams of authors independently spelling it  surgy ? ![file] ,