Lidocaine alleviates cytotoxicity-resistance in lung cancer A549/DDP cells via down-regulation of miR-21
Authors: Chuangen Ma
Haixia Xu
Qing Yang
Zhi Zhang
Journal: Molecular and Cellular Biochemistry (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Cisplatin; Lidocaine; Lung cancer; MicroRNA-21; PDCD4/JNK
Affiliations: Department of Anesthesiology, Huaihe Hospital of Henan University, No. 8, Baobei Road, Gulou District, Kaifeng, 475000, China., Department of Anesthesiology, Huaihe Hospital of Henan University, No. 8, Baobei Road, Gulou District, Kaifeng, 475000, China. .

Abstract:  Lidocaine (Lido) is a commonly used local anesthetic, which has been reported in various types of cells. However, the effects of Lido on lung cancer cells remain not understood. The study aimed to investigate the underlying mechanisms of Lido in the cisplatin resistance of A549/DDP cells. Different concentrations of cisplatin (0-320 µM) were used to stimulate A549 and A549/DDP cells, and cell viability and apoptosis were examined. To investigate the effect of Lido on A549/DDP cells, the optimum concentration of Lido was selected to treat A549/DDP cells, and cell viability, apoptosis, migration and invasion were then detected. The relative expression of miR-21 in A549/DDP cells or in Lido-treated A549/DDP cells was analyzed by RT-qPCR. MiR-21 mimic, inhibitor and its control were transfected into A549/DDP cells to explore the regulatory effect of miR-21 on the cisplatin resistance in A549 or A549/DDP cells. The effects of miR-21 on PTEN/PI3K/AKT and PDCD4/JNK pathways were detected by western blot. The cisplatin resistance of A549/DDP cells was higher than that of A549 cells. Lido significantly suppressed cell viability, induced apoptosis, and inhibited cell migration and invasion in A549/DDP cells. Additionally, miR-21 expression in A549/DDP was higher than that in A549 cells, and Lido significantly down-regulated miR-21 expression in A549/DDP cells. MmiR-21 inhibition exhibited the same effects as Lido on the cisplatin resistance of A549/DDP cells. Further, miR-21 suppression regulated PTEN/PI3K/AKT and PDCD4/JNK pathways in A549/DDP cells. These findings indicated that Lido alleviated the cytotoxicity resistance of A549/DDP cells via down-regulation of miR-21. 

Retracted: No

PubPeer comments:
 The authors can boast of an eclectic range of affiliations: - C.-Y.J. Chen: Department of Civil Engineering, Universidade de Brasu00edlia, Brasu00edlia, 70910-900 Distrito Federal, Brazil - Chia-Yen Hsieh: Special Education, National Kaohsiung Normal University, Kaohsiung - Aiden Smith: Faculty of Engineering, Universidad del Norte, Barranquilla, AA 1569 Atlantico, Colombia - Dariush Alako: Department of Natural Science, Bost University, Lashkargah Helmand, Afghanistan Afghanistan - Lallit Pandey: Applied Math Department, St. George s University, Grenada - Tim Chen: AI Lab, Faculty of Information Technology, Ton Duc Thang University, Ho Chi Minh City, Vietnam It was [elsewhere observed that]  The author J.C.-Y. (or C.Y.J.) Chen exhibited **29 different academic affiliations** in different institutes and or universities in 15 different countries (i.e. Bangladesh, Nigeria, Saudi Arabia, United States, Papua New Guinea, Taiwan, United Kingdom, Brasilia, Australia, China, Russia, Kazakhstan, Vietnam, Spain, Egypt) for the 68 papers (at least) published between 2018 and 2021 in which he is a co-author with Tim Chen (timchen@tdtu.edu.vn). Author Chia-Yen Hsieh is assigned an affiliation to Special Education, National Kaohsiung Normal University. There is a Dr Chia-Yen Hsieh at [a different institution] , with his **degree** in Special Education from National Kaohsiung Normal University. That other C.-Y. Hsieh has also collaborated with Tim Chen ( Effect of Pokeu00b4mon GO on the Cognitive Performance and Emotional Intelligence of Primary School Students : [Hsieh Chen, 2019] ), but his research interests lie far from  ocean systems engineering . Authors Chen and Chen do have prior form for [inventing or identity-stealing co-authors] . Background [here] . ![] , 
  Lallit Pandey: Applied Math Department, St. George s University, Grenada There is no Applied Math Department at St. George s University. , 
 So far **Table 1** and **Figure 1** (see below) are re-used in respectively **4 other papers** and **2 other papers** written by the **same two authors Tim Chen and C.Y.J alias John Chen**. ![file] ![file] u00b0 **Table 1** was published in [Chen, Khurram Cheng (2019)] , [Chen _et al._(2021)] , [Chen, Kuo Chen (2021)] and [Chen Chen (2021)] . u00b0 **Figure 1** was published in [Chen, Khurram Cheng (2019)] and [Chen Chen (2021)] . As stated in a [previous post] , this figure is from [Tsai et al. (2016)] . ![file] ,