miR-199a-3p inhibits aurora kinase A and attenuates prostate cancer growth: new avenue for prostate cancer treatment
Authors: Jinlin Wu
Xiang Huang
Fengyan Zhao
Zhiqing Li
Junyan Liu
Yi Qu
Dapeng Chen
Dezhi Mu
Journal: American Journal Of Pathology (2014)
Discipline: CLINICAL MEDICINE
Keywords: Prostate cancer; Surgery; Aurora kinase A; miRNAs; Gene expression
Affiliations: Key Laboratory of Birth Defects of Ministry of Education, Sichuan University, Chengdu, China. Electronic address: ., Department of Urology, The People's Hospital of Sichuan Province, Chengdu, China., Key Laboratory of Birth Defects of Ministry of Education, Sichuan University, Chengdu, China.

Abstract:  Prostate cancer (PCa) is the most common solid tumor malignancy in men that severely influences quality of life. Surgery is most often the recommended treatment for PCa, but radical prostatectomy can cause significant urinary adverse effects. Therefore, finding effective biochemical treatments for PCa remains a necessity. Aurora kinase A has been shown to be involved in PCa progression, thus making it a good target for PCa therapy. miRNAs are important regulators of gene expression, with some miRNAs specifically involved in carcinogenesis. Therefore, herein, we identified miRNAs targeted to aurora kinase A and examined their effects on the growth of PCa. We used primary samples from PCa patients and PCa cell lines as research subjects. We demonstrate that miR-199a-3p is down-regulated in PCa tissues compared with normal prostate tissues, with the expression pattern inversely correlated with the expression pattern of aurora kinase A. We find that miR-199a-3p agomir inhibits aurora kinase A and attenuates xenograft tumor growth of PCa. Moreover, we demonstrate that down-regulation of miR-199a-3p results from enhancement of the methylation of miR-199a gene in PCa. Furthermore, we find that the expression level of miR-199a-3p is inversely correlated to tumor stage and Gleason score of PCa. Revealing novel mechanisms for oncogene inhibition by miRNA-mediated pathways offers new avenues for PCa treatment. 

Retracted: No

PubPeer comments:
 Figure 3B. Most panels appear to show bands surrounded by different shades of grey, suggesting they came from different gels with different backgrounds. This makes it hard to analyze their sizes or relative intensities. Bands also appear to differ in sharpness and  smiling orientation. Of note, the 24h b-actin panel might be upside down. Could the authors please show the original blots? ![file] , 
 Figure 3C. I am worried about the peaks shown here. In each of the four panels, the rightmost dark grey peak looks like a disheveled conifer after a windstorm. These peaks, in particular those from the right three panels, also appear to share similarities, as highlighted here with red boxes. Could the authors please assure us that these peaks are totally fine? I am really worried about how they look, and just wanted to make sure they are ok. ![file] , 
 Dear Dr. Bik, Thank you very much for pointing out this mistake of Figurer 3 C of the paper. I am the co-corresponding author of the paper. I have contacted the first author and the first corresponding author to carefully check the figure and will response to you as soon as possible. Sincerely, Dezhi Mu , 
 Dear Dr. Bik, Iu2019m the first author, Yi Qu, of the paper. Thank you very much for pointing out our errors. We are now checking the original data, especially the figures from the collaborating units. , 
 Dear Dr. Bik, Iu2019m the first author of the paper. Thank you very much for pointing out our mistakes . We are now checking the original data, especially the figures from the collaborating units. ,