MiR-155 regulates the proliferation and apoptosis of pancreatic cancer cells through targeting SOCS3
Authors: J Wang
J Guo
H Fan
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: SOCS3; miR-155; pancreatic cancer; expression; regulation
Affiliations: Department of Hepatobiliary Surgery, Huangshi Central Hospital of the Eastern Hubei Medical Group, Huangshi, Hubei, China. .

Abstract:  Objective: Reduced expression of suppressors of cytokine signaling 3 (SOCS3) is associated with a variety of tumors. The elevated miR-155 expression is associated with the onset of pancreatic cancer. Bioinformatics analysis revealed a targeted relation between miR-155 and the 3'-UTR of SOCS3. This study investigated whether miR-155 regulates SOCS3 expression and affects the biological effects of pancreatic cancer cells. Patients and methods: QRT-PCR was used to detect the expression of miR-155 and SCOS3 mRNA in tumor tissues and paracancerous tissues of patients with pancreatic cancer. The dual luciferase reporter gene assay validated the target interaction between miR-155 and SOCS3. Pancreatic cancer cell line SW1990 cells were divided into miR-NC group and miR-155 inhibitor group followed by an analysis of the expressions of SOCS3, p-JAK2 and p-STAT3, cell apoptosis by flow cytometry, and cell proliferation by EdU staining. Results: Compared with adjacent tissues, miR-155 expression was increased in tumor tissues of patients with pancreatic cancer, and SOCS3 expression was decreased. There was a targeted regulatory relationship between miR-155 and SOCS3 mRNA. Compared with HPDE6-C7 cells, miR-155 expression in pancreatic cancer SW1990 and Capan-1 cells was increased, and SOCS3 expression was decreased. Transfection of miR-155 inhibitor significantly increased SOCS3 expression in pancreatic cancer SW1990 cells, decreased the expression of p-JAK2 and p-STAT3, increased cell apoptosis, and decreased cell proliferation. Conclusions: Increased miR-155 expression and decreased SOCS3 expression are related to the pathogenesis of pancreatic cancer. miR-155 inhibits the proliferation and apoptosis of pancreatic cancer cells by inhibition of SOCS3 expression. 

Retracted: Yes

PubPeer comments:
 Why would  Corresponding Author Hui Fan, MD use the e-address  songlinzhanw@yeah.net for academic correspondence? Did this not pique the editors interest? , 
 Right:  Figure 4. Inhibition of **miR-155** expression can significantly inhibit **pancreatic cancer** cell proliferation and promote apoptosis. .. E, Flow detection of apoptosis . Left:  Figure 4. Overexpression of **miR-29** can significantly inhibit **osteosarcoma** cell proliferation and induce apoptosis. ... D, Flow cytometry to detect apoptosis in SJSA-1 cells . From [Li et al (2019)] . ![file] , 
 Please appreciate the effort I put into creating this Gallery of Unfortunate FACS Plots. Fig 4D [Li et al (2019)] 10.26355 eurrev_201909_18832 Fig 5D [Liu et al (2019)] 10.26355 eurrev_201911_19421 Fig 4E [Du et al (2019)] 10.26355 eurrev_201910_19278 Fig 4D [Dong et al (2019)] 10.26355 eurrev_201907_18305 ![file] Fig 4E [Wu et al (2019)] 10.26355 eurrev_201909_18852 Fig 3C [Cao et al (2019)] 10.26355 eurrev_201908_18531 Fig 4E [Wang et al (2019)] 10.26355 eurrev_201906_18181 Fig 4C [Wei et al (2019)] 10.26355 eurrev_201907_18299 ![file] , 
 #3 These eight papers also display a strange stylised form of Western Blots, where out-of-focus geometrical ellipses hover above a featureless background. Shadowy wisps of smoke hang in the foreground but there is no real-world detail. Did I mention the absence of overlap among the different teams of authors, and their far-flung locations? Figs 3C, 4C [Li et al (2019)] 10.26355 eurrev_201909_18832 Figs 4B, 5B [Liu et al (2019)] 10.26355 eurrev_201911_19421 Figs 3C, 4C [Du et al (2019)] 10.26355 eurrev_201910_19278 Figs 3C, 4C [Dong et al (2019)] 10.26355 eurrev_201907_18305 ![file] Figs 2B, 4C [Wu et al (2019)] 10.26355 eurrev_201909_18852 Figs 2B, 3B [Cao et al (2019)] 10.26355 eurrev_201908_18531 Figs 3B, 4C [Wang et al (2019)] 10.26355 eurrev_201906_18181 Figs 3B, 4B [Wei et al (2019)] 10.26355 eurrev_201907_18299 ![file] , 
 Now retracted: https: www.europeanreview.org wp wp-content uploads 12625-12625.pdf ------------------------------- Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cMiR-155 regulates the proliferation and apoptosis of pancreatic cancer cells through targeting SOCS3, by J. Wang, J. Guo, H. Fan, published in Eur Rev Med Pharmacol Sci 2019; 23 (12): 5168-5175u2013DOI: 10.26355 eurrev_201906_18181u2013PMID: 31298410u201d has been withdrawn. The authors stated that the problem was related to u201csubstantial deficiency in the experimental design (Figures 3B and 4C, 4E, and 4F). The experiment was not done well at that time. It was processed by computer and there were some inaccuraciesu201d. The Publisher apologizes for any inconvenience this may cause. ,