Taenia solium and Taenia crassiceps: miRNomes of the larvae and effects of miR-10-5p and let-7-5p on murine peritoneal macrophages
Authors: Luz Navarro
Abraham Landa
Lucía Jiménez
Alicia Ochoa-Sánchez
Journal: Bioscience Reports (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Gene regulation; Larvae; Macrophages; Taenia solium; microRNA (miRs).
Affiliations: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, CDMX 04510, Mexico., Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, CDMX 04510, México.

Abstract:  Neurocysticercosis (NCC), a major cause of neurological morbidity worldwide, is caused by the larvae of Taenia solium. Cestodes secrete molecules that block the Th1 response of their hosts and induce a Th2 response permissive to their establishment. Mature microRNAs (miRs) are small noncoding RNAs that regulate gene expression and participate in immunological processes. To determine the participation of Taenia miRs in the immune response against cysticercosis, we constructed small RNA (sRNA) libraries from larvae of Taenia solium and Taenia crassiceps. A total of 12074504 and 11779456 sequencing reads for T. solium and T. crassiceps, respectively, were mapped to the genomes of T. solium and other helminths. Both larvae shared similar miRNome, and miR-10-5p was the most abundant in both species, followed by let-7-5p in T. solium and miR-4989-3p in T. crassiceps, whereas among the genus-specific miRs, miR-001-3p was the most abundant in both, followed by miR-002-3p in T. solium and miR-003a-3p in T. crassiceps. The sequences of these miRs were identical in both. Structure and target prediction analyses revealed that these pre-miRs formed a hairpin and had more than one target involved in immunoregulation. Culture of macrophages, RT-PCR and ELISA assays showed that cells internalized miR-10-5p and let-7-5p into the cytoplasm and the miRs strongly decreased interleukin 16 (Il6) expression, tumor necrosis factor (TNF) and IL-12 secretion, and moderately decreased nitric oxide synthase inducible (Nos2) and Il1b expression (pro-inflammatory cytokines) in M(IFN-γ) macrophages and expression of Tgf1b, and the secretion of IL-10 (anti-inflammatory cytokines) in M(IL-4) macrophages. These findings could help us understand the role of miRs in the host-Taenia relationship. 

Retracted: No

PubPeer comments:
 One of the GAPDH bands in Figure 7A appears to have a splice (red arrow) that is not readily apparent in the other corresponding bands. Could the authors please provide uncropped original scans of this gel? *** ![file] , 
 I m sorry for the delay in responding, the comment notice came to the spam folder and a new notice came to me yesterday. I ll be sending the originals on Tuesday, since my university is closed by the pandemic and I need to go through the files that are being protected on the lab computer. Please wait for the files on Tuesday 28. , 
 #2 Thank you for your reply. I look forward to hearing the results of your investigation. , 
 #3 I sent the documents this Tuesday, July 28 via email to Zara Manwaring Managing Editor Biochemical Society. Portland Press in response to request. Best regards , 
 Correction June 28, 2021 The authors of the original article u201cTaenia solium and Taenia crassiceps: miRNomes of the larvae and effects of miR-10-5p and let-7-5p on murine peritoneal macrophagesu201d (Biosci Rep (2019) 39(11) BSR20190152), have realised that a splice had been introduced in Figure 7A of their article during their figure preparation. The original files used to create this figure have been assessed and approved by the Deputy Editor-in-Chief and the Editorial Board, and a version without this splice is presented below. The authors would also like to correct some typos in Table 3 of their article. The correct version of their table is also present in this Correction. ,