MicroRNA-206 inhibition and activation of the AMPK/Nampt signalling pathway enhance sevoflurane post-conditioning-induced amelioration of myocardial ischaemia/reperfusion injury
Authors: Ke Wang
Shan-Shan Yu
Chang-Yu Zhou
Tao Li
Ying-Chun Wan
Journal: Journal of Drug Targeting (2020)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: AMPK/Nampt signalling pathway; MicroRNA-206; autophagy; myocardial ischaemia/reperfusion injury; sevoflurane post-conditioning.
Affiliations: Department of Anesthesiology, China-Japan Union Hospital of Jilin University, Changchun, P.R. China;, Department of Gastroenterology, China-Japan Union Hospital of Jilin University, Changchun, P.R. China;, Department of Gynaecology and Obstetrics, China-Japan Union Hospital of Jilin University, Changchun, P.R. China;, Department of Endocrinology, China-Japan Union Hospital of Jilin University, Changchun, P.R. China

Abstract:  This study aimed to investigate the effect of miR-206 on apoptosis and autophagy of cardiomyocytes in ischaemia/reperfusion (I/R) injured rats treated with sevoflurane post-conditioning (SP) through the AMPK/Nampt signalling pathway. Rat models of myocardial I/R injury were established. The combination of SP, miR-206 inhibitor, AMPK activator AICAR and inhibitor Compound C was induced in rats, and their effects on I/R injury were determined with detection of malondialdehyde (MDA), hydroxyproline (HYP) and superoxide dismutase (SOD) levels in cardiomyocytes, autophagy, and apoptosis. We also conducted experiments to determine p62, Beclin1, Bax and Bcl-2 expression and the mRNA and expression pattern of AMPK/Nampt signalling pathway-related genes. Myocardial I/R injured rats revealed decreased SOD activity and elevated MDA content, autophagy and apoptosis. With the combined performance of SP, miR-206 inhibitor and AMPK activator AICAR, the rats presented higher SOD level and lower MDA and HYP levels, suppressed autophagy and apoptosis. Meanwhile, miR-206 inhibition also contributed to elevated expression of Nampt and the extent of AMPK phosphorylation, increased Bcl-2/Bax ratio, but degraded expression of p62 and Beclin1. Collectively, inhibition of miR-206 could activate the AMPK/Nampt signalling pathway, thus protecting against myocardial I/R injury on the basis of SP. 

Retracted: Yes

PubPeer comments:
 Two images in Figure 3A appear to overlap (red boxes). This type of issue sometimes appears in papers that have been outsourced. Last author name seems to be the same on recently retracted paper discussed here: https: pubpeer.com publications D1F1F637053684BC53A804787BA2B5. *** ![file] *** , 
 Retracted [19 May 2022] . -------------------------------- We, the Editor and Publishers of Journal of Drug Targeting, have retracted the following article: Tao Li, Shan-Shan Yu, Chang-Yu Zhou, Ke Wang, Ying-Chun Wan. MicroRNA-206 inhibition and activation of the AMPK Nampt signalling pathway enhance sevoflurane post-conditioning-induced amelioration of myocardial ischaemia reperfusion injury. J Drug Target. 2019;28(1):80u201391. DOI: 10.1080 1061186X.2019.1616744 Following publication, concerns were raised regarding the alleged duplication of images in Figure 3A in which regions of panel SPu2009+u2009miR-206 inhibitor appeared to be duplicated with regions of panel SPu2009+u2009AICAR. The authors did not respond to our queries and were unable to provide their original data or any necessary supporting information. As verifying the validity of published work is core to the integrity of the scholarly record, we are therefore retracting the article. All authors listed in this publication have been informed of this decision. We have been informed in our decision-making by our policy on publishing ethics and integrity and the COPE guidelines on retractions. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as u2018Retractedu2019. ,