<p>Enhanced Antitumor Efficacy of Macrophage-Mediated Egg Yolk Lipid-Derived Delivery System Against Breast Cancer</p>
Authors: Yali Jun
Qilong Wang
Li Zhang
Zhuang Tang
Mingyue Tao
Zhengwei Zhang
Lu Liu
Xiang Li
Chao Luo
Su&#x27;An Sun
Yanguan Lv
Journal: International Journal of Nanomedicine (2020)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: breast cancer; drug delivery; egg yolk lipid-derived nanovector; macrophage; target therapy.
Affiliations: Department of Clinical Medical Laboratory, Huai'an Maternity and Child Healthcare Hospital Affiliated to Yangzhou University Medical Academy, Huai'an 223002, People's Republic of China., Department of Central Laboratory, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an 223300, People's Republic of China., School of Pharmacy and State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, 999078, People's Republic of China., Department of Clinical Oncology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an 223300, People's Republic of China., Department of Pathology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huai'an 223300, People's Republic of China.

Abstract:  Background: Chemotherapy is the primary treatment for most cancers apart from surgery. However, the use of chemotherapeutic drugs is limited by side effects and restricted accumulation in tumors because of unique tumor microenvironments. Macrophages have excellent drug delivery potential owing to their chemotaxis and can home in on tumors. Materials and methods: We developed an effective drug-delivery system for doxorubicin using macrophages. Doxorubicin-loaded egg yolk lipid-derived nanovectors (EYLNs-Dox) were prepared, EYLNs-Dox-loaded macrophages (Mac/EYLNs-Dox) were developed and their tumor penetration and anti-cancer activity against 4T1 cells were analyzed. The biodistribution and anti-4T1 breast cancer activities were determined using 4T1 subcutaneous and lung metastasis models. Results: EYLNs-Dox was successfully internalized into macrophages without affecting their viability and was less toxic than Dox. Mac/EYLNs-Dox penetrated the 4T1 tumor spheroids more efficiently and was more effective in inhibiting tumors in vitro. Macrophages significantly enhanced the distribution of EYLNs vectors in both inflammatory and tumor sites, playing a more effective role in the inhibition of tumors. Conclusion: EYLNs-Dox can be effectively delivered using macrophages and Mac/EYLNs-Dox might be a promising targeted delivery system for breast cancer. 

Retracted: No

PubPeer comments:
 Two sets of mice images appear to be repeated, although they seem to have different tumor signals suggesting this error did not occur during figure assembly. *** ![file] , 
 #1 Dear Dr. Actinopolyspora Biskrensis, I appreciate your interest in our work. We carefully checked our manuscript and raw data again and found the images you mentioned were misused in the manuscript editing process. Attached please find the corrected images for figure 5c. ![file] I most sincerely apologize for our editing mistakes and I will use this as an invaluable learning chance to do better in safeguarding the accuracy and integrity of science. We have contacted the editor board to revise the images. Yours sincerely, Chao Luo! , 
 We have contacted the journal and completed the errata. , 
 Manipulation of data should not be corrected, it should be retracted. ,