Upregulating miR-146a by physcion reverses multidrug resistance in human chronic myelogenous leukemia K562/ADM cells
Authors: Juan He
Qulian Guo
Fei Gao
Wenjun Liu
Yiling Yang
Journal: American journal of cancer research (2016)
Discipline: CLINICAL MEDICINE
Keywords: CXCR4; chronic myeloid leukemia; drug resistance; miR-146a; physcion.
Affiliations: Department of Pediatrics, Affiliated Hospital of Southwest Medical University Luzhou 646000, Sichuan, China.

Abstract:  The aim of this study was to evaluate the role of miR-146a in the drug resistance of chronic myelogenous leukemia (CML) cells (K562/ADM) and to investigate the reversal effect of physcion, a natural compound, on the multidrug-resistance in CML. Our results showed that miR-146a was significantly downregulated in drug-resistant K562 cells and the overexpression of miR-146a in K562/ADM cells could restore the sensitivity to adriamycin (ADM). In addition, our results showed that the downregulation of miR-146a was associated with increase in CXCR4 expression, which was a direct target of miR-146a. Moreover, our findings also provided experimental evidence that physcion could enhance the anti-proliferative effect of ADM in K562/ADM cells by upregulating miR-146a. In conclusion, this present study showed that miR-146a conferred ADM resistance in CML cells and physcion could improve the sensitivity of K562/ADM cells by enhancing apoptosis via upregulating miR-146a. 

Retracted: Yes

PubPeer comments:
 Fig 6B:  Physcion potentiates the anti-tumor effect of ADM in vivo. ... B. Combination of physcion and ADM shows more significant inhibiting effect on tumor growth.  ![file] Later reincarnated as Fig 5B of [Du et al (2019)] :  Anti-tumor activities of PG in vivo. ... (B) PG dose-dependently decreased the [lung] tumor weight. ![] At right, Fig 4A from [Pan et al (2019)] ,  Combination therapy of physcion and sorafenib significantly suppresses the [hepatocellular] tumor growth in vivo. (A) Combination of physcion and sorafenib can markedly suppress tumor growth . At left, Fig 7C, from [Liu et al (2017)] ,  AF suppresses ovarian tumor growth in vivo. .... C. Tumor weight measurement showed exertion of anti-tumor effects in vivo by AF. ![] , 
 Fing 3  D. Knockdown of CXCR4 in K562 cells transfected with miR-146a inhibitor restores the sensitivity to ADM-induced apoptosis . ![file] When two panels are identical in the two upper quadrants, it is corrosive of one s confidence that they really represent the results of separate experiments. , 
 #1 The  Physcion + ADM tumors panel had earlier illustrated how high-dose hispidulin slowed the growth of colon carcinoma xenografts, in Fig 7D ( Tumors representing each of the experimental groups ) of [Xie et al (2015)] . ![] ![file] , 
 Fig 5F [below]  Physcion reverses the resistance of K562 ADM cells through upregulating miR-146a and interfering CXC12 CXCR4 signaling. .... F. Physcion dose-dependently interferes with the binding between CXC12 and CXCR4 . [Above] Fig. 2E from [Zhang et al (2016)] .  MiR-124 functions as tumor suppressor in melanoma cells. ... E. MiR-124 inhibits invasion while miR-124 promotes invasion in melanoma cells . ![] , 
 Retracted [1 August 2020] .  A large portion of the data and the text in this article was copied from another source as disclosed by a knowledgeable informant and subsequently confirmed by the Editorial Office. Therefore, the entire article has been retracted in accordance with this journalu2019s policy and editorial decision. ,