Long noncoding RNA MT1JP inhibits proliferation, invasion, and migration while promoting apoptosis of glioma cells through the activation of PTEN/Akt signaling pathway
Authors: Hanyang Liang
Haozhe Piao
Ji Shi
Ye Zhang
Yi Chen
Rui Sui
Journal: Journal of Cellular Physiology (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: IncRNA MT1JP; PTEN/Akt signaling pathway; apoptosis; glioma; invasion
Affiliations: Department of Neurosurgery Liaoning Cancer Hospital &amp; Insititute, Cancer Hospital of China Medical University Shenyang Liaoning People's Republic of China

Abstract:  This study is carried out to elucidate the role of long noncoding RNAs (lncRNAs) MT1JP in proliferation, invasion, migration, and apoptosis of glioma cells through the regulation of PTEN/Akt signaling pathway. The expression of MT1JP in 80 normal brain tissues and 138 glioma tissues, as well as glioma cell lines, was detected by quantitative reverse-transcription polymerase chain reaction. Besides, glioma cells with overexpression and low expression of MT1JP were constructed to confirm the role of MT1JP in proliferation, invasion, migration, and apoptosis of glioma cells and the growth of glioma cells in vivo through the regulation of PTEN/Akt signaling pathway. MT1JP expression was downregulated in glioma tissues and cells. The low expression of MT1JP was considered as an independent risk factor for predicting overall survival in gliomas. After transfection of MT1JP overexpression plasmid, glioma cells showed decreased proliferation, migration and invasion ability, increased apoptosis rate, and decreased the tumorigenic ability of nude mice. The trends were opposite in glioma cells transfected with MT1JP poor expression plasmid. Collectively, our study suggests that lncRNA MT1JP is responsible for inhibiting proliferation, invasion, and migration while promoting apoptosis of glioma cells through the activation of PTEN/Akt signaling pathway. 

Retracted: Yes

PubPeer comments:
 Figure 6 contains what appear to be duplicate images(rotated). ![file] Figure 5 appears to have a similar issue, but most importantly also appears to have a duplicate image with another paper (DOI: 10.1038 srep44688): Figure 5 from this paper: ![file] Figure 8 from Qiao et al 2017: ![file] Can the authors explain this? , 
 Fig 6(a) again: rotating one panel... ![file] ...for better comparison with Fig 5B from [Xie et al (2017)] . ![file] , 
 Fig 4(d).  Detection of cell apoptosis in each group by flow cytometry . The central regions of the pEGFP-NC and shRNA-NC plots are more similar around the meeting-point of the quadrants than I would expect. The shRNA-NC and pGPU6-MT1JP-shRNA plots are rife with repeating elements. I lost the will to live before I could examine other plots. ![file] , 
 Fig 6A again. ![file] Figs 5A,B, 7A,B of [Xu et al (2015)] . ![file] , 
 Retracted [20 July 2021] . ---------------------------------------------- The above article, published online on 07 May 2019 in Wiley Online Library , has been retracted by agreement between the journal s Editor in Chief, Prof. Dr. Gregg Fields, and Wiley Periodicals LLC. The retraction has been agreed following an investigation based on allegations raised by a third party. Several flaws and inconsistencies between results presented and experimental methods described were found. Thus, the editors consider the conclusions of this article to be invalid. ,