miRNA-1207-5p is associated with cancer progression by targeting stomatin-like protein 2 in esophageal carcinoma
Authors: Wenqiao Zang
Zhongquan Wang
Guoqiang Zhao
Xiaoyan Xuan
Zhicai Liu
Jing Cui
Jinwu Wang
Xuan Yang
Journal: International Journal of Oncology (2015)
Discipline: CLINICAL MEDICINE
Keywords: miRNA-1207-5p; esophageal carcinoma; STOML-2; gene expression; cancer
Affiliations: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, P.R. China., Tumor Hospital of Linzhou City, Linzhou, Henan 456500, P.R. China.

Abstract:  Newly discovered intrinsic regulators, the miRNAs regulate gene expression by binding to the 3'-untranslated regions of the genome. Accumulating studies have indicated that miRNAs are aberrantly expressed in various human cancers. We found that miRNA-1207-5p (miR‑1207-5p) was markedly downregulated in esophageal carcinoma (EC) tissues, and was correlated with EC differentiation, pathological stage and lymph node metastasis. Rates of apoptosis were increased and cell invasion ability was decreased in EC9706 and EC-1 cells transfected with a miR‑1207-5p mimic. Stomatin-like protein 2 (STOML-2) was predicted to be a potential target of miR‑1207-5p by bioinformatics analysis and this was confirmed by luciferase assay and western blotting. Our study showed that STOML-2 was negatively regulated by miR‑1207-5p. Furthermore, overexpression of STOML-2 abolished the miR‑1207-5p anti-invasion function. Based on these results, we proposed that miR‑1207-5p might act as a potential therapeutic target in the treatment of EC. 

Retracted: No

PubPeer comments:
 Corrigendum, Jan 2018: https: www.spandidos-publications.com 10.3892 ijo.2018.4254  An interested reader drew to our attention that, in the above-mentioned article, there were two figures where identity in certain of the data was shared between panels within the same figure. First, in Fig. 3B, the data shown for the EC9706 cell line negative control (NC) experiment were derived from the same original source as those for the EC-1 Blank control experiment. Secondly, in Fig. 6B the Bcl-2 bands for the two different cell lines, EC9706 and EC-1, were inadvertently duplicated (the data shown for the EC9706 cell line were correct). These corrections do not address a problem in Figure 3A. Specifically, the two upper left FACS plots show many cells (points) in common. Superimposing the two panels (with one black white reversed, and semitransparent, so that matching points cancel out) results in cancellation throughout the lower left quadrant. Superimposing them with a different alignment results in cancellation through the upper right quadrant. ![file] , 
 Top left:  Figure 3. Effect of miR-125a-5p mimic on the invasive capacity of A549 and H1299 cells using Transwell assay. (A) The Transwell images of A549 and H1299 cell lines. From  miR-125a-5p upregulation suppresses the proliferation and induces the cell apoptosis of lung adenocarcinoma by targeting NEDD9 [(Zheng et al 2017)] . Top right:  Figure 3. Effects of upregulation of miR-1207-5p on apoptosis and invasion of EC9706 and EC-1....(B) Transwell assays were used to assess the invasive ability. Areas of matching cell colonies marked with colored rectangles, and paired up along the bottom. Note, though that there are occasional colonies in the 2017 versions that are not present in the 2015 versions, as if something has happened to the images as well as accidental reuse. ![] , 
 #2 Note also an overlap between a panel of Figure 3B (at bottom) and a panel of Figure 5 in [Bai et al (2017)] (at top). ![file] This is a corollary of the overlap between figures in [Zheng et al (2017)] and Bai et al (2017). , 
 Unexpected similarities between two pairs of FACS plots. Below, Fig 3A. Top here is Figure 4 from  Long noncoding RNA PEG10 regulates proliferation and invasion of esophageal cancer cells [(Zang et al 2015).] . ![file] , 
 [Perhaps a systemic problem] . Journals and readers may wish to proceed with caution. ,