LncRNA SAMD12-AS1 down-regulates P53 to promote malignant progression of glioma
Authors: Y-L Du
L Zou
S Li
B-X Yu
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: LncRNA; SAMD12-AS1; glioma; invasion; migration
Affiliations: Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Suzhou, China. .

Abstract:  Objective: To study the expression level of long non-coding RNA (LncRNA) SAMD12-AS1 in glioma and its influence on the invasive ability of glioma cells; meanwhile, the regulation mechanism of LncRNA SAMD12-AS1 promoting the development of glioma was further explored. Patients and methods: The expression level of SAMD12-AS1 in 40 pairs of tumor tissue specimens and paracancerous ones collected from glioma patients were examined through Real Time quantitative-Polymerase Chain Reaction (qRT-PCR) method, and the interplay between SAMD12-AS1 expression and clinical indicators of glioma patients was also analyzed. Meanwhile, the expression of SAMD12-AS1 in glioma cell lines was further verified by qRT-PCR. In addition, SAMD12-AS1 knockdown model was constructed using glioma cell lines (T98-G and U87). Cell Counting Kit-8 (CCK-8), cell wound healing test, and transwell assays were conducted to examine the impact of SAMD12-AS on glioma cell functions. Additionally, whether it exerted its biological characteristics through P53 was finally explored. Results: qPCR results in the study revealed that SAMD12-AS1 expression in tumor tissue specimens of glioma patients was remarkably higher than that in the adjacent ones, and the difference was statistically significant. Compared with patients with low expression of SAMD12-AS1, patients with high expression of SAMD1-AS1 had a higher incidence of lymph node or distant metastasis. In addition, compared to the NC group, knocking down SAMD12-AS1 markedly attenuated the proliferation rate, as well as the invasiveness and migration ability of glioma cells. Subsequently, in glioma tissues, it was verified that P53 expression was remarkably decreased and negatively correlated with SAMD12-AS1. Finally, cell recovery experiment also demonstrated that there may exist a mutual regulation between SAMD12-AS1 and P53, which then together affected the malignant progression of glioma. Conclusions: LncRNA SAMD12-AS1 may accelerate the invasion and migratory capacities of glioma cells by modulating P53, and its expression was confirmed to be significantly relevant to the incidence of lymph node or distant metastasis. 

Retracted: No

PubPeer comments:
 Readers should become aware of a preprint entitled  The potato NLR immune receptor R3a does not contain non-canonical integrated domains that reanalysed data from Kroj et al., 2016 paper https: www.biorxiv.org content 10.1101 056242v1 From the Abstract:  **A recent study by Kroj et al. (New Phytologist, 2016) surveyed nucleotide binding-leucine rich repeat (NLR) proteins from plant genomes for the presence of extraneous integrated domains that may serve as decoys or sensors for pathogen effectors. They reported that a FAM75 domain of unknown function occurs near the C-terminus of the potato late blight NLR protein R3a. Here, we investigated in detail the domain architecture of the R3a protein, its potato paralog R3b, and their tomato ortholog I2. We conclude that the R3a, R3b, and I2 proteins do not carry additional domains besides the classic NLR modules, and that the FAM75 domain match is likely a false positive among computationally predicted NLR-integrated domains.** ,