miR-182-5p Attenuates High-Fat -Diet-Induced Nonalcoholic Steatohepatitis in Mice
Authors: Huan Chen
Xiaoqun Xu
Weiwei Jiang
Qionghe Liang
Journal: Annals of Hepatology (2019)
Discipline: CLINICAL MEDICINE
Keywords: Non-alcoholic fatty liver disease; Non-alcoholic steatohepatitis; Toll-like receptor 4; miR-182-5p.
Affiliations: Department of Neonatal Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China; Department of Radiology, Children's Hospital of Nanjing Medical University, Nanjing, China., Department of Neonatal Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China., Department of Neonatal Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China; Institute of Pediatric Research, Children's Hospital of Nanjing Medical University, Nanjing, China. Electronic address: .

Abstract:  Introduction and aim: Patients with NASH have increased risk for sepsis or cardiovascular disease after Liver transplantation. An important role of Toll-like receptor (TLR) 4 in the pathogenesis of nonalcoholic steatohepatitis (NASH) was demonstrated. Here, we study the role of miR-182-5p in TLR4 expression and high-fat-diet (HFD)-induced NASH in vitro and in vivo Material and methods. Following transfection with a miR-182-5p mimic, the effect of miR-182-5p on TLR4 in RAW264.7 and HepG2 cells was investigated. Following administration of the miR-182-5p mimic into the livers of HFD-induced NASH mice, we determined the in vivo expression of TLR4, TNFa, and IL-6 and assessed the histologic features of the livers. Results Following lipopolysaccharide (LPS) treatment of RAW264.7 cells, real-time RT-PCR and western blot results indicated decreases levels of TLR4 mRNA and protein in the miR-182-5p group as compared with levels observed in controls, with similar trends were observed in TNFa and IL-6 protein levels. Following oleic acid (OA) treatment of HepG2 cells, TLR4, TNFa, and IL-6 levels were significantly decreased in the miR-182-5p group as compared with levels observed in controls. Following miR-182-5p administration, TLR4 mRNA and protein levels decreased along with those of TNFa and IL-6 proteins, and the liver weight/body weight ratio of treated mice was less than that observed in controls. Furthermore, hematoxylin and eosin staining showed that the miR-182-5p-treated group exhibited low adiposecell cross-sectional areas, and Oil Red O staining showed decreases in the size of lipid droplets in the miR-182-5p-treated group. Conclusions: miR-182-5p ameliorated HFD-induced NASH by suppressing TLR4. 

Retracted: No

PubPeer comments:
 Figure 1B. Panels B (RAW264.7 cells) and C (HepG2 cells) appear to look remarkably similar in terms of the height of the bars and the error bars. The only difference is the error bar in the 2nd bar from the left which appears to not be positioned in the middle of the bar, as if it was hand drawn (marked by me with an aqua circle). ![file] , 
 Figures 2B and 4B. * Red boxes: The TLR4 panels appear to look very similar. ![file] , 
 Dear Prof Elisabeth M Bik Thank you very much for your careful review. The first histogram (Figure 1b) is indeed misplaced in the group diagram. Figure 1a and figure 1b are the same. It s because the same picture was put in when copying and pasting, but figure 1b was not copieduff0cso it s wrong. When the article was accepted, the picture is the same as the one on the attached picture (black).. The blue picture was redone by the magazine, so it turned into the blue one now. We will contact the magazine to correct the mistakes. I m very sorry for the trouble. The second problem, I found a problem after careful study. The two protein maps in the paper should be the same strip produced by different exposures, and they were put in the same folder. Due to the similar marks, there was an error in the selection of the mapuff0cso there was an error in the selection of the map. I regret that. We will actively contact the magazine for revision.![](http: ) Thank you very much. I will review it strictly and check it carefully in the future. Weiwei Jiang![file] ,