MiR-129-5p is down-regulated and involved in migration and invasion of gastric cancer cells by targeting interleukin-8
Authors: N Ma
S Chen
Z Zong
Y Li
Z Jiang
W Chen
H Wang
Z Hou
Journal: Neoplasma (2016)
Discipline: CLINICAL MEDICINE
Keywords: gastric cancer; interleukin-8.; microRNA-129-5p; migration and invasion.

Abstract:  Expression of microRNA-129-5p (miR-129-5p) has been reported to decrease in gastric cancer (GC). However, little information is available about how miR-129-5p affects cell migration and invasion of GC. Cancer samples and matched non-tumor adjacent tissues were obtained from patients with GC. Besides, peripheral blood samples were collected from both the patients and healthy volunteers. Expression of miR-129-5p was analyzed by real-time PCR (RT-PCR). After transfection with miR-129-5p mimics, miR-129-5p inhibitor, or negative controls in human GC cell line SGC-7901, cell viability, colony-formation ability, migration, and invasion assay were evaluated. Luciferase reporter assay, RT-PCR, and enzyme-linked immunosorbent assay (ELISA) were performed to explore whether interleukin-8 was a target of miR-129-5p. Further, small interfering RNA (siRNA) against IL-8 was transfected into cells, and then the effects of miR-129-5p inhibitor on migration and invasion were assessed. MiR-129-5p was down-regulated in both GC samples and blood samples compared to their matched non-tumor adjacent tissues and healthy volunteers (both P &lt; 0.05). Compared to the control group, transfection with miR-129-5p inhibitor markedly increased the cell viability, colony-forming ability, and numbers of migrated and invaded cells. Luciferase reporter assay confirmed that IL-8 was a direct target of miR-129-5p, and IL-8 was negatively regulated by miR-129-5p. Co-transfection of miR-129-5p inhibitor with si-IL-8 reversed the effect of miR-129-5p inhibitor on the migration and invasion of the cells. MiR-129-5p and regulates migration and invasion of GC cells by targeting IL-8. 

Retracted: No

PubPeer comments:
 2019 retraction notice. https: journals.plos.org plosone article?id=10.1371 journal.pone.0210894 After publication of this article [1], several concerns were raised about images presented in Figures 1B and 2B. Similarities were noted between the following images in Figure 1B: ccnD1 lanes 1, 2 and wnt5a lanes 2, 1 (horizontally flipped) ccnD1 lanes 1u20133 and tgfB1r1 lanes 2u20134 tgfB1r1 lane 1 and wnt5a lane 3 (horizontally flipped) wnt3 lanes 1u20134 and wnt7a lanes 1u20134 The following similarities were noted in the upper panels of Figure 2B: PKC A B panel: CMT-U27 cells, lane 1 (ctrl), CMT-U309 cells lane 1 (ctrl; horizontally flipped), and P114 lane 2 (CM, horizontally flipped) PKC A B panel: CMT-U27 lane 2 (CM) and P114 lane 1 (ctrl; horizontally flipped) PKC A B panel: CMT-U309 lane 2 (CM) and P114 lane 3 (+MQ) Akt panel: CMT-U27 lanes 1, 2 (ctrl, CM) and CMT-U309 lanes 2, 3 (CM, +MQ), respectively (for these, the bands appear similar but with different intensity suggesting different exposures) Akt panel: CMT-U27 lane 3 (+MQ) and P114 lane 1 (ctrl) JNK 1 3 panel: CMT-U27 cells, lane 3 (+MQ) and CMT-U309 panel lane 3 (+MQ; horizontally flipped) Calmodulin panel: CMT-U309 lane 1 (ctrl) and P114 lane 1 (ctrl; horizontally flipped) In the lower panels of Figure 2B, it was noted that the band and background in lane 3 of the Lamin a c CMT-U27 panel (+MQ) appears highly similar to those in lane 1 of the Lamin a c CMT-U309 panel (ctrl). For these, the bands and background differ in intensity across the panels, suggesting they represent different exposures. We raised these concerns with the authors, who provided some raw data supporting results in Figures 1 and 2, but noted that the original images supporting the figure panels in question are no longer available. The above similarities call into question the integrity and reliability of the results reported in these figures. The concerns cannot be resolved in light of the data unavailability. Furthermore, the article is not in compliance with the journalu2019s Data Availability policy that was in place at the time of the articleu2019s publication. In light of these concerns, the PLOS ONE Editors retract this article. JM, KM, AH, MB, AM, MG, EH agreed with the retraction. M. Pietrzak, M. Perszko, KR, KP, TM did not respond. MK did not agree with retraction. EM could not be reached. Reference 1. Kru00f3l M, Mucha J, Majchrzak K, Homa A, Bulkowska M, Majewska A, et al. (2014) Macrophages Mediate a Switch between Canonical and Non-Canonical Wnt Pathways in Canine Mammary Tumors. PLoS ONE 9(1): e83995. https: doi.org 10.1371 journal.pone.0083995 pmid:24404146 , 
 An illustration of the concerns in Figure 1B.  Similarities were noted between the following images in Figure 1B: * ccnD1 lanes 1, 2 and wnt5a lanes 2, 1 (horizontally flipped) * ccnD1 lanes 1u20133 and tgfB1r1 lanes 2u20134 * tgfB1r1 lane 1 and wnt5a lane 3 (horizontally flipped) * wnt3 lanes 1u20134 and wnt7a lanes 1u20134 ![file] , 
 An illustration of the concerns in Figure 2B.  The following similarities were noted in the upper panels of Figure 2B: * PKC A B panel: CMT-U27 cells, lane 1 (ctrl), CMT-U309 cells lane 1 (ctrl; horizontally flipped), and P114 lane 2 (CM, horizontally flipped) * PKC A B panel: CMT-U27 lane 2 (CM) and P114 lane 1 (ctrl; horizontally flipped) PKC A B panel: CMT-U309 lane 2 (CM) and P114 lane 3 (+MQ) * Akt panel: CMT-U27 lanes 1, 2 (ctrl, CM) and CMT-U309 lanes 2, 3 (CM, +MQ), respectively (for these, the bands appear similar but with different intensity suggesting different exposures) * Akt panel: CMT-U27 lane 3 (+MQ) and P114 lane 1 (ctrl) JNK 1 3 panel: CMT-U27 cells, lane 3 (+MQ) and CMT-U309 panel lane 3 (+MQ; horizontally flipped) * Calmodulin panel: CMT-U309 lane 1 (ctrl) and P114 lane 1 (ctrl; horizontally flipped) * In the lower panels of Figure 2B, it was noted that the band and background in lane 3 of the Lamin a c CMT-U27 panel (+MQ) appears highly similar to those in lane 1 of the Lamin a c CMT-U309 panel (ctrl). For these, the bands and background differ in intensity across the panels, suggesting they represent different exposures. ![file] ,