MiR-129 reduces CDDP resistance in gastric cancer cells by inhibiting MAPK3
Authors: C-Y Guo
H-J Lu
H-Z Yu
C-H Xiao
H-Y Cao
H Hong
J-F Yuan
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: MAPK3; miR-129; gastric cancer; drug resistance; proliferation
Affiliations: Department of Clinical Laboratory, Affiliated Traditional Chinese Medicine Hospital of Nantong University, Nantong, Jiangsu, China. .

Abstract:  Objective: Abnormal expression of mitogen-activated protein kinase 3 (MAPK3) is related to invasion, metastasis, and drug resistance of multiple tumor cells. MiR-129 expression is associated with gastric cancer. Bioinformatics analysis showed a targeting relation between miR-129 and MAPK3. This study investigated whether miR-129 plays a role in regulating MAPK3 expression, affecting proliferation, apoptosis, and cisplatin (CDDP) resistance of gastric cancer cells. Materials and methods: The dual-luciferase reporter gene assay was used to assess the targeted regulation between miR-129 and MAPK3. The expression of miR-129 and MAPK3 in CDDP-resistant cell line MGC-803/CDDP and the parental MGC-803 cells was measured. MGC-803/CDDP cells were cultured in vitro and divided into miR-NC group and miR-129 mimic group. The expression of MAPK3 and p-MAPK3 protein were detected by Western blot and the effect of CDDP treatment on cell apoptosis and proliferation was detected by flow cytometry. Results: There was a targeted regulation relation between miR-129 and MAPK3 mRNA. MiR-129 expression in MGC-803/CDDP cells was significantly lower than that in MGC-803 cells and the expression of MAPK3 mRNA and protein was significantly higher than that in MGC-803 cells. Compared with miR-NC group, the expression of MAPK3 and p-MAPK3 in MHC-803/CDDP cells in miR-129 mimic transfection group was significantly decreased, with increased cell apoptosis and reduced cell proliferation. Conclusions: The decreased expression of miR-129 and the up-regulation of MAPK3 are associated with CDDP resistance in gastric cancer cells. Overexpression of miR-129 inhibits MAPK3 expression and cell proliferation, it induces cell apoptosis and reduces CDDP resistance. 

Retracted: Yes

PubPeer comments:
 Please appreciate the effort I put into creating this Gallery of Unfortunate FACS Plots. Fig 4D [Li et al (2019)] 10.26355 eurrev_201909_18832 Fig 5D [Liu et al (2019)] 10.26355 eurrev_201911_19421 Fig 4E [Du et al (2019)] 10.26355 eurrev_201910_19278 Fig 4D [Dong et al (2019)] 10.26355 eurrev_201907_18305 ![file] Fig 4E [Wu et al (2019)] 10.26355 eurrev_201909_18852 Fig 3C [Cao et al (2019)] 10.26355 eurrev_201908_18531 Fig 4E [Wang et al (2019)] 10.26355 eurrev_201906_18181 Fig 4C [Wei et al (2019)] 10.26355 eurrev_201907_18299 ![file] , 
 These eight papers also display a strange stylised form of Western Blots, where out-of-focus geometrical ellipses hover above a featureless background. Shadowy wisps of smoke hang in the foreground but there is no real-world detail. Did I mention the absence of overlap among the different teams of authors, and their far-flung locations? Figs 3C, 4C [Li et al (2019)] 10.26355 eurrev_201909_18832 Figs 4B, 5B [Liu et al (2019)] 10.26355 eurrev_201911_19421 Figs 3C, 4C [Du et al (2019)] 10.26355 eurrev_201910_19278 Figs 3C, 4C [Dong et al (2019)] 10.26355 eurrev_201907_18305 ![file] Figs 2B, 4C [Wu et al (2019)] 10.26355 eurrev_201909_18852 Figs 2B, 3B [Cao et al (2019)] 10.26355 eurrev_201908_18531 Figs 3B, 4C [Wang et al (2019)] 10.26355 eurrev_201906_18181 Figs 3B, 4B [Wei et al (2019)] 10.26355 eurrev_201907_18299 ![file] , 
 Withdrawn: https: www.europeanreview.org article 23761 *Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cMiR-129 reduces CDDP resistance in gastric cancer cells by inhibiting MAPK3, by H.-Y. Cao, C.-H. Xiao, H.-J. Lu, H.-Z. Yu, H. Hong, C.-Y. Guo, J.-F. Yuan, published in Eur Rev Med Pharmacol Sci 2019; 23 (15): 6478-6485u2013DOI: 10.26355 eurrev_201908_18531u2013PMID: 31378887u201d has been withdrawn.* *The Publisher apologizes for any inconvenience this may cause.* , 
 Nantong Cancer Hospital announced the results of an investigation on [September 16 2021](http: www.ntzlyy.cn news 1211.html). Thanks to Google Translate for the translation. ----------------- In order to further strengthen warning education, strengthen the construction of scientific research integrity, severely punish violations, and create a good environment for scientific and technological innovation, we hereby report the relevant paper issues recently investigated and dealt with by our unit as follows: Nantong Traditional Chinese Medicine Hospital Yu Haizhong is the corresponding author, Nantong Cancer Hospital Xiao Chunhong is the co-corresponding author, and Nantong Traditional Chinese Medicine Hospital Cao Hongyan is the first author. The paper  miR-129 reduces CDDP resistance in gastric is published in European Review for Medical and Pharmacological Sciences.  Cancer cells by inhibiting MAPK3 was investigated, and Comrade Xiao Chunhong, as the co-corresponding author, used the fund number  No.MS12017017-4 in violation of regulations and tampered with the data, which is an academic misconduct. Nantong Tumor Hospital decided to give Comrade Xiao Chunhong the following treatment after research: - 1. Suspend the application of qualifications for financially funded science and technology plans (special projects, funds, etc.) for 8 years, and recover relevant paper awards; - 2. Announce criticism; - 3. Experiments are carried out in earnest to verify, fill up missing materials and pictures; - 4. The hospital will further strengthen scientific research supervision and improve related management systems to ensure the normal development of scientific research. After research, Nantong Hospital of Traditional Chinese Medicine decided to give Comrade Yu Haizhong the following treatment: - 1. Announce criticism; - 2. Withdraw the above-mentioned papers published; - 3. Cancel the qualifications for applying for financially funded science and technology plans (special projects, funds, etc.) for 8 years (involving other paper frauds) , Combined punishment), to recover the one-time bonus for the paper; - 4. Rectify and strengthen the management of the academic team, and cancel the qualification of the academic committee. Nantong Traditional Chinese Medicine gave Comrade Cao Hongyan the following treatments: - 1. Scientific research integrity and admonition talk; - 2. Strengthen scientific research integrity education and strengthen data storage awareness education. ,