Long noncoding RNA Pvt1 regulates the immunosuppression activity of granulocytic myeloid-derived suppressor cells in tumor-bearing mice
Authors: Jie Ma
Xinyu Tian
Xueli Xia
Jie Tian
Huaxi Xu
Ping Xu
Shengjun Wang
Tingting Wang
Fenghua Cao
Yu Zheng
Journal: Molecular Cancer (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Immunosuppression; Long noncoding RNA; Myeloid-derived suppressor cells; Pvt1.
Affiliations: Department of Laboratory Medicine, the Affiliated People's Hospital, Jiangsu University, Zhenjiang, 212013, China., Department of Immunology, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, China., Department of Laboratory Medicine, the Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi Children's Hospital, Wuxi, China., Department of Laboratory Medicine, Zhenjiang Hospital of Chinese Traditional and Western Medicine, Zhenjiang, China., Department of Immunology, Jiangsu Key Laboratory of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, China. ., Department of Laboratory Medicine, The Fifth People's Hospital of Suzhou, Suzhou, China. ., Department of Laboratory Medicine, the Affiliated People's Hospital, Jiangsu University, Zhenjiang, 212013, China. .

Abstract:  Background: Myeloid-derived suppressor cells (MDSCs) participate in tumor-elicited immunosuppression by dramatically blocking T-cell-induced antitumor responses, thereby influencing the effectiveness of cancer immunotherapies. Treatments that alter the differentiation and function of MDSCs can partially restore antitumor immune responses. The long noncoding RNA plasmacytoma variant translocation 1 (lncRNA Pvt1) is a potential oncogene in a variety of cancer types. However, whether lncRNA Pvt1 is involved in the regulation of MDSCs has not been thoroughly elucidated to date. Methods: MDSCs or granulocytic MDSCs (G-MDSCs) were isolated by microbeads and flow cytometry. Bone marrow derived G-MDSCs were induced by IL-6 and GM-CSF. The expression of lncRNA Pvt1 was measured by qRT-PCR. Specific siRNA was used to knockdown the expression of lncRNA Pvt1 in G-MDSCs. Results: In this study, we found that knockdown of lncRNA Pvt1 significantly inhibited the immunosuppressive function of G-MDSCs in vitro. Additionally, lncRNA Pvt1 knockdown reduced the ability of G-MDSCs to delay tumor progression in tumor-bearing mice in vivo. Notably, lncRNA Pvt1 was upregulated by HIF-1α under hypoxia in G-MDSCs. Conclusions: Taken together, our results demonstrate a critical role for lncRNA Pvt1 in regulating the immunosuppression activity of G-MDSCs, and lncRNA Pvt1 might thus be a potential antitumor immunotherapy target. 

Retracted: No

PubPeer comments:
 Fig 3B.  Overexpression of miR-128 inhibited AXIN1 to promote EAAT4 expression in dopamine neurons. ...B and C, Western blot analysis of the protein level of AXIN1 and EAAT4 . ![file] I am intrigued by the absence of background on these WBs, and the way the bands remain perfectly linear and retain the shape of the protein blobs as they move up the gel. Is it possible for the authors to upload the original scans of the gels? Numerous other papers have Figures which display the same qualities, hence my curiosity. I compiled a montage: ![file] , 
 ![file] Hello Hoya Thanks for your comments. I am the corresponding author WF, and sorry for the delayed reply due to a long holiday. Attached is the scan of original glue for WB figure with issue on this website. , 
 #2 Thank you! Concerns allayed! ,