MicroRNA-101 inhibits growth and metastasis of human ovarian cancer cells by targeting PI3K/AKT
Authors: Zhuo Li
Min Wei
Xinlei Wang
LiXiang Li
Hongjuan Jin
ShuLi Yang
Yan Jia
ManHua Cui
Journal: Archives of Medical Science (2021)
Discipline: CLINICAL MEDICINE
Keywords: apoptosis; microRNA; ovarian cancer; proliferation.
Affiliations: Department of Gynecology and Obstetrics, The Second Hospital of Jilin University, Changchun, Jilin, China, Department of Plastic and Reconstructive Surgery, The First Hospital of Jilin University, Changchun, Jilin, China

Abstract:  Introduction: Ovarian cancer is the most frequent cause of gynecological cancer related mortality in woman. This study was designed to investigate the role and therapeutic potential of miRNA-101 in ovarian cancer. Material and methods: Expression analysis was carried out by real-time quantitative polymerase chain reaction. Transfections were performed with the help of Lipofectamine 2000 reagent. AO/EB and annexin V/PI staining was used to detect apoptosis and flow cytometry was used for cell cycle analysis. Western blotting was employed for cell cycle analysis. Results: It was found that miRNA-101 was significantly down-regulated in ovarian cancer cells. The over-expression of miRNA-101 causes a significant decrease in the viability of ovarian cancer cells via the initiation of apoptosis and sub-G1 arrest of OVACAR-3 cells. It was indicated that PTEN was the potential target of miRNA-101 in OVACAR-3 cells. There was 4.5-fold up-regulation of PTEN expression in ovarian cancer cell lines and the over-expression of miRNA-101 in OVACAR-3 cells resulted in the down-regulation of PTEN expression. The inhibition of PTEN in the OVACAR-3 cells arrested the proliferation of these cells. The over-expression of miRNA-101 causes significant down-regulation in PI3K and AKT expression of OVACAR-3 cells. Conclusions: It can be concluded that miRNA-101 acts as a tumor suppressor which may be beneficial in the treatment of ovarian cancer. 

Retracted: No

PubPeer comments:
 *[below]* Fig 2A from [Wei et al (2019)] .  Overexpression of miRNA-101 triggers apoptosis in ovarian cancer cells. A u2013 AO EB staining showing induction of apoptosis in NC or miRNA-101 mimic transfected OVACAR-3 ovarian cancer cells. *[above]* Figure 2 from [Cao et al (2020)] .  A u2013 miR-NC and miR-27a inhibitor transfected A431 cells showing morphology as revealed by phase contrast microscopy, induction of apoptosis as depicted by AO EB staining... ![file] , 
 Thank you for pointing it out. Our manuscript was published one year ago than the other one. , 
 Fig 2B from [Wei et al (2021)] .  Annexin V PI staining showing percentage of apoptosis in NC or miRNA-101 mimic transfected OVACAR-3 ovarian cancer cells. ![file] For comparison: Fig 2D from [Zhong et al (2019)] .  Overexpression of miR-324 triggers apoptosis in KGN ovarian granulosa cells of PCOS,... (D) Annexin V PI staining of miR-324 in NC- and miR-324 mimics-transfected KNG cells. ![] *[left]* Fig 3B from [Wang et al (2020)] . *[right]* Figure 2C from [Chen, Lu Hu (2019)] .  A: AO EB staining, B: DAPI staining, C: Annexin V PI staining of the miR-NC and miR-299 mimic-transfected HK1 nasopharyngeal cancer cells . ![] ,