miR‚Ä?09‚Ä?p suppresses the proliferation, invasion and migration of tongue squamous cell carcinoma via targeting RDX
Authors: Jing Dai
Hujie Chen
Journal: Oncology Letters (2018)
Discipline: CLINICAL MEDICINE
Keywords: invasion; miR-409-3p; migration; proliferation; radixin
Affiliations: Department of Stomatology, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, Hubei 434000, P.R. China

Abstract:  The aim of the present study is to investigate the role of microRNA (miRNA/miR)-409-3p in the proliferation, invasion and migration of tongue squamous cell carcinoma (TSCC) cells via targeting radixin (RDX) gene. The expression of miR-409-3p was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in TSCC tissue and cell lines. The binding of miR-409-3p to RDX was investigated by performing a dual-luciferase reporter gene assay. Tca8113 cells were selected to transfect with miR-409-3p mimic/inhibitor, small interfering (si)-RDX, and miR-409-3p inhibitor + si-RDX, as well as negative control (NC) respectively. The proliferative, migratory and invasive abilities of transfected Tca8113 cells were investigated by cell-counting-kit-8, wound-healing and Transwell assays, respectively. Additionally, a tumor xenograft model was constructed to examine the effects of miR-409-3p on the tumor growth and lymphatic metastasis in nude mice. A significant downregulation was detected in miR-409-3p expression in TSCC tissues and cells (all P&lt;0.05) compared with normal tongue mucosa tissues and cell line, which was associated with lymph node metastasis and tumor-node metastasis staging (both P&lt;0.05). The results from the dual-luciferase reporter gene assay indicated that RDX is a potential target gene of miR-409-3p. Compared with the blank group, a marked reduction in RDX expression, cell proliferation, migration and invasion was detected in the miR-409-3p mimic group and si-RDX group (all P&lt;0.05). Conversely, the reverse was observed in cells that were transfected with the miR-409-3p inhibitor. Furthermore, si-RDX is able to reverse the effect of miR-409-3p inhibitor on cell proliferation, invasion and migration (all P&lt;0.05). The results form the tumor xenograft model of nude mice verified that miR-409-3p mimic is able to inhibit the growth of Tca8113 tumor cells and lymph node metastasis in nude mice. miR-409-3p may delay the proliferation of TSCC cells by inhibiting of RDX so as to decrease its migratory and invasive abilities. Therefore, miR-409-3p may be a potential target for the clinical treatment of TSCC. 

Retracted: No

PubPeer comments:
 The images in this paper are unexpectedly similar to those in other papers. Figure 3 of this paper:  Figure 12 and 11 of [Li 2015](http: pubpeer.com publications B3DE3BD6ED69BF2D2D0EAA458B757F) :   Figure 9 of [Qiao 2017](http: pubpeer.com publications A2B9F909208655184858F1F3293580) :  Figure 4 of [Bai 2016](http: pubpeer.com publications DD6F9374BD9CB9FDE5B2BE8E9C0102) :  Figure 5 of [Xie 2017](http: pubpeer.com publications 5FF3FA303B55D3696508D6BFE715A4) :  Figure 5 of [Ba 2017](http: pubpeer.com publications AEFB35F8F72B292D427FFDD6F360D9) :  Thank you to Hoya Camphorifolia for finding and posting many of these papers. ,