Inhibitory effects of 90Sr/90Y Œ≤-irradiation on alkali burn-induced corneal neovascularization in rats
Authors: Yuanqiang Lin
Qiang Wen
Shi Gao
Qingjie Ma
Shan Lin
Hongyan Zhou
Guanghui Cheng
Journal: Experimental and Therapeutic Medicine (2016)
Discipline: CLINICAL MEDICINE
Keywords: 90Sr-90Y; alkali burn; corneal; neovascularization; Œ≤-irradiation.

Abstract:  The aim of the present study was to investigate the inhibitory effects of 90Sr-90Y Œ≤-irradiation in a rat model of alkali burn-induced corneal neovascularization (CNV). Alkali burn-induced CNV was induced in the right eyes of 30 female Wistar rats, which were randomly divided into the following three groups (n=10/group): i) The alkali burn control group, which received a balanced salt solution treatment; ii) group 1, which received treatment with angiogenesis inhibitors; and iii) group 2, which received 90Sr-90Y Œ≤-irradiation treatment. A further 10 female Wistar rats comprised a blank control group and received only balanced salt solution. Digital photographs of the corneas were acquired and the area of NV was calculated. In addition, the expression levels of matrix metalloproteinase (MMP)-9, vascular endothelial growth factor (VEGF), VEGF receptor (VEGFR)-1 and VEGFR-2 in alkali-burned rat corneas were determined using western blot analysis. The results suggested that the number of new vessels and the area of CNV were significantly decreased in groups 1 and 2, as compared with the alkali burn group at each time point (P&lt;0.05). In addition, the number of inflammatory cells and the degree of edema were decreased in groups 1 and 2, as compared with the alkali burn group, with group 2 exhibiting the most marked reduction. Western blot analysis demonstrated that the expression levels of MMP-9, VEGF, VEGFR-1 and VEGFR-2 were significantly decreased in groups 1 and 2, as compared with the alkali burn control group, with group 2 exhibiting the most significant reduction (P&lt;0.05). The results of the present study suggested that 90Sr-90Y Œ≤-irradiation and angiogenesis inhibitor treatments were able to inhibit alkali burn-induced CNV, although 90Sr-90Y Œ≤-irradiation may be more effective. 

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PubPeer comments:
 Fig 3.  Effect of 90Sr-90Y u03b2-irradiation treatment on the protein expression of neovascularization-related signal molecules in the cornea. u03b2-Actin served as an internal standard. The alkali burn control group was treated with balanced salt solution. Group 1 received angiogenesis inhibitors treatment and group 2 90Sr-90Y u03b2-irradiation treatment.  These are very versatile bands. Compare, e.g., *[left]* Fig 3C from [Ge et al (2016)] .  Western blot analysis of uPA, MMP-2 and MMP-9 protein expression following transfection with miR-497 mimic or miR-control. GAPDH was used as an internal control . *[right]* Figs 3C,E from [Liu et al (2015)] .  The ... (C) protein expression levels of PTEN were detected in the HCC cells after transfection with miR-494 mimic or inhibitor. The PTEN protein expression level was detected in... (E) HCC cell lines by western blotting.  Other manifestations are marked in a spreadsheet by rows with  G2 in the  WBs column: https: docs.google.com spreadsheets d 1-6xhXnvVjKASgfCITEF15IsI54PczGt4Yp6rKOXaHhI edit?usp=sharing , 
 Corresponding author *Professor Ma Qingjie is the director, chief physician, professor, and doctoral supervisor of the Department of Nuclear Medicine, China-Japan Friendship Hospital of Jilin University. Engaged in clinical work for more than 30 years. He has expertise and innovation in the personalized treatment of human thyroid diseases, various types of pediatric hemangioma, and certain tumor diseases; for multimodal molecules such as PET CT and SPECT CT He has deep attainments in imaging research and tumor-targeted drug therapy. He was named  Bethune Famous Doctor ,  Bethune Famous Teacher ,  Bethune Medical Worker ,  Expert with Outstanding Contributions in Changchun City, Jilin University. In 2019, he won the Chinese Nuclear Medicine Physician Award.* ,