MiR-155 regulates lymphoma cell proliferation and apoptosis through targeting SOCS3/JAK-STAT3 signaling pathway
Authors: X-D Li
X-C Sun
X-M Li
J-W Gu
Journal: European review for medical and pharmacological sciences (2017)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: JAK-STAT signaling pathway; SOCS3; miR-155; lymphoma; DLBCL
Affiliations: Dental Department, Yan'an People's Hospital of Shaanxi Province, Yan'an, Shaanxi, China. .

Abstract:  Objective: Janus kinase (JAK)- signal transducer and activator of transcription (STAT) signaling pathway participates in regulating cell proliferation, differentiation, and apoptosis, and related to lymphoma. Suppressors of cytokine signaling 3 (SOCS3) is a negative regulator of the JAK-STAT signaling pathway. SOCS3 reduction and miR-155 up-regulation are associated with lymphoma pathogenesis. Bioinformatics analysis showed the complementary binding site between miR-155 and SOCS3. This study aimed to investigate the role of miR-155 in regulating SOCS3/JAK-STAT signaling pathway and affecting diffuse large B cell lymphoma (DLBCL) cell proliferation and apoptosis. Patients and methods: DLBCL tumor sample was collected from the patients in our hospital. Lymphatic tissue derived from reactive lymphoid hyperplasia patients were selected as control. MicroRNA-155 (MiR-155) and SOCS3 expressions were detected. Dual luciferase assay was used to verify the targeted relationship between miR-155 and SOCS3. OCI-LY10 cells were cultured in vitro and divided into five groups, including miR-NC, miR-155 inhibitor, pIRES2-Blank, pIRES2-SOCS3, and miR-155 + pIRES2-SOCS3 groups. SOCS3, p-JAK1, p-JAK2, p-STAT3, and Survivin expressions were tested. Cell apoptosis and proliferation were detected by flow cytometry. Results: MiR-155 expression significantly increased, while SOCS3 level declined in DLBCL tissue compared with control. MiR-155 targeted regulated SOCS3 expression. MiR-155 inhibitor and/or pIRES2-SOCS3 transfection markedly up-regulated SOCS3 expression, reduced p-JAK1, p-JAK2, p-STAT3, and Survivin levels, attenuated cell proliferation, and enhanced cell apoptosis in OCI-LY10 cells. Conclusions: Down-regulation of miR-155 inhibited DLBCL cell proliferation and facilitated apoptosis through up-regulating SOCS3 expression to suppress JAK-STAT3 signaling pathway. 

Retracted: Yes

PubPeer comments:
 This paper was one of many listed as the work of a papermill, on account of its stylistic features. https: docs.google.com spreadsheets d 1qBqQMXgzxoBd5bTbqdMuapGheupE5bdIopypju417NM edit#gid=0 Some time in the last week or so, the journal wished it into the cornfield: https: www.europeanreview.org article 22251 Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cMiR-155 regulates lymphoma cell proliferation and apoptosis through targeting SOCS3 JAK-STAT3 signaling pathway, by X.-D. Li, X.-M. Li, J.-W. Gu, X.-C. Sun, published in Eur Rev Med Pharmacol Sci 2017; 21 (22): 5153-5159u2013DOI: 10.26355 eurrev_201711_13832u2013PMID: 29228427u201d has been withdrawn. The Publisher apologizes for any inconvenience this may cause.  ,