MicroRNA-129-5p alleviates nerve injury and inflammatory response of Alzheimer‚Äôs disease via downregulating SOX6
Authors: Simiao Zhang
Linlin Hua
Qian Yuan
Honglei Yin
Hongying Bai
Yajun Liu
Xiaodong Li
Liubin Liu
Shanshan Wang
Jianhao Xiao
Zhen Wang
Yunliang Wang
Wei Zheng
Zhilei Zeng
Journal: Cell Cycle (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Interleukin-1Œ≤; MicroRNA-129-5p; SOX6.
Affiliations: Department of Neurology, The Second Affiliated Hospital of Zhengzhou University , Zhengzhou , Henan , China., Department of Neurology, The 960th PLA hospital , Zibo , shandong , China.

Abstract:  There is growing evidence of the position of microRNAs (miRs) in Alzheimer's disease (AD), thus our objective was to discuss the impact of miR-129-5p regulating nerve injury and inflammatory response in AD rats by modulating SOX6 expression. The AD rat model was established by injecting AŒ≤25-35 into the brain. The pathological changes, ultrastructure, number of neurons, cell degeneration and apoptosis of hippocampal tissue were observed in vivo. MiR-129-5p, SOX6, IL-1Œ≤, TNF-Œ±, Bcl-2 and Bax expression in serum and hippocampal tissues were detected by ELISA, RT-qPCR or western blot analysis. The successfully modeled hippocampal neuronal cells of AD were transfected with miR-129-5p mimic, SOX6-siRNA or their controls to figure out their roles in proliferation, apoptosis and inflammatory reaction in vitro. Low expression of SOX6 and high expression of miR-129-5p in vivo of rats would shorten the escape latent period and increase the times of crossing platforms, alleviate the pathological injury, inhibit neuronal apoptosis and reduce the inflammatory reaction. Up-regulation of miR-129-5p and down-regulation of SOX6 promoted proliferation, suppressed apoptosis and degraded the inflammatory reaction of neuronal cells in vitro. Up-regulation of SOX6 reversed the expression of miR-129-5p to reduce the damage and inflammatory response of the cell model of AD. Our study presents that up-regulation of miR-129-5p or down-regulation of SOX6 can reduce nerve injury and inflammatory response in rats with AD. Thus, miR-129-5p may be a potential candidate for the treatment of AD. 

Retracted: Yes

PubPeer comments:
 Fig 3b.  Observation results of neuronal apoptosis by TUNEL staining . I have marked some of the repeating visual elements involved in this composition.  , 
 Fig 5a.  Morphological observation of the hippocampus neurons in rat .  I m sure these images were fun to create, but they have no scientific value. , 
 Retracted [17 March 2022] . -------------------------------- We, the Editors and Publisher of the journal *Cell Cycle*, have retracted the following article: Zhilei Zeng, Yajun Liu, Wei Zheng, Liubin Liu, Honglei Yin, Simiao Zhang, Hongying Bai, Linlin Hua, Shanshan Wang, Zhen Wang, Xiaodong Li, Jianhao Xiao, Qian Yuan, andYunliang Wang. MicroRNA-129-5p alleviates nerve injury and inflammatory response of Alzheimeru2019s disease via downregulating SOX6. Cell Cycle. 2019;18(22):3095-3110. doi: 10.1080 15384101.2019.1669388. Since publication, concerns have been raised about the integrity of the data in the article. When approached for an explanation, the authors checked their data and confirmed there are fundamental errors present. Therefore, they have agreed to the retraction of this article. The authors apologise for this oversight. We have been informed in our decision-making by our policy on publishing ethics and integrity and the COPE guidelines on retractions. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as u2018Retractedu2019. ,