Immobilization of lactoperoxidase on ZnO nanoparticles with improved stability
Authors: Mehrnaz Movahedi
Seyed Ziyae Aldin Samsam Shariat
Mehrdad Movahedi
Habibollah Nazem
Journal: Biotechnology letters (2020)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: Glutaraldehyde; Immobilization; Lactoperoxidase; Stability; ZnO nanoparticles.
Affiliations: Department of Biology, Payame Noor University, P.O. Box 19395-4697, Tehran, Iran. ., Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran., Department of Biology, Payame Noor University, P.O. Box 19395-4697, Tehran, Iran., Department of Geology, Faculty of Earth Sciences, Shahid Beheshti University, Tehran, Iran.

Abstract:  Objectives: The study aimed to develop a facile and effectual method to enhance the stability of lactoperoxidase (LPO) by immobilizing it on ZnO Nanoparticles (ZnO NPs). Results: The successful immobilization of LPO on ZnO NPs was confirmed by using Fourier transform infrared spectroscopy (FT-IR) and field emission scanning electron microscopy (FE-SEM). The Km values of free LPO and LPO immobilized on ZnO were 53.19, 89.28 mM and their Vmax values were 0.629, 0.46 ¬µmol/mL min, respectively. The overall results showed that the stability of the immobilized LPO was significantly improved compared to free LPO. The LPO immobilized on ZnO (LPO-ZnO) retained 18% of the initial activity within 30 days at 25 ¬∞C whereas the free enzyme lost its activity after 7 days at the same temperature. Moreover, evaluation of the thermal stability of LPO at 75 ¬∞C determined the conservation of 12% of the initial activity of LPO in the LPO-ZnO sample after 60 min whereas the free enzyme lost its activity after 5 min. Conclusions: According to the present results, ZnO nanoparticles are suitable for the immobilization of LPO. 

Retracted: Yes

PubPeer comments:
 2020 retraction. https: link.springer.com article 10.1007 s10529-020-02888-z Retraction to: Biotechnology Letters (2020) 42:737u2013745 https: doi.org 10.1007 s10529-020-02828-x The Editors have retracted this article [1] because significant parts of the text were duplicated from previously published articles by Ziyae et al. 2019 [2], Movahedi et al. 2019 [3] and Ziyae et al. 2018 [4]. The authors have not responded to any correspondence regarding this retraction. References 1. Movahedi M, Samsam Shariat SZA, Nazem H, Movahedi M (2020) Immobilization of lactoperoxidase on ZnO nanoparticles with improved stability. Biotechnol Lett. https: doi.org 10.1007 s10529-020-02828-x 2. Samsam Shariat SZA, Movahedi M, Nazem H (2019) Immobilization of lactoperoxidase on Fe3O4 magnetic nanoparticles with improved stability. Biotechnol Lett 41:1373u20131382 https: doi.org 10.1007 s10529-019-02741-y 3. Movahedi M, Samsam Shariat SZA, Nazem H (2019) Immobilization of lactoperoxidase on graphene oxide nanosheets and copper oxide nanoparticles and evaluation of their stability. Catal Lett 149:562u2013573 https: doi.org 10.1007 s10562-018-2620-0 4. Samsam Shariat SZA, Borzouee E, Mofid MR, Varshosaz J (2018) Immobilization of lactoperoxidase on graphene oxide nanosheets with improved activity and stability. Biotechnol Lett 40:1343u20131353 https: doi.org 10.1007 s10529-018-2583-7 ,