Effects of unpredictable chronic stress on behavior and brain-derived neurotrophic factor expression in CA3 subfield and dentate gyrus of the hippocampus in different aged rats
Authors: Qian Zhang
Yong-juan Ji
Ying Li
Shu-jian Fan
Hong Jiang
Fang Pan
De-xiang Liu
Journal: Chinese Medical Journal (2009)
Discipline: CLINICAL MEDICINE
Keywords: Brain-derived neurotrophic factor (BDNF); stress; age; behavior; hippocampus
Affiliations: Institute of Medical Psychology, School of Medicine, Shandong University, Jinan, Shandong 250012, China.

Abstract:  Background: Brain-derived neurotrophic factor (BDNF) is a stress-responsive intercellular messenger modifying hypothalamic-pituitary-adrenal (HPA) axis activity. The interaction between stress and age in BDNF expression is currently not fully understood. This study was conducted to observe unpredictable stress effect on behavior and BDNF expression in CA3 subfield (CA3) and dentate gyrus of hippocampus in different aged rats. Methods: Forty-eight Wistar rats of two different ages (2 months and 15 months) were randomly assigned to six groups: two control groups and four stress groups. The rats in the stress group received three weeks of unpredictable mild stress. The depression state and the stress level of the animals were determined by sucrose preference test and observation of exploratory behavior in an open field (OF) test. The expressions of BDNF in CA3 and dentate gyrus of the hippocampus were measured using immunohistochemistry. Results: Age and stress had different effects on the behavior of different aged animals (age: F = 6.173, P &lt; 0.05, stress: F = 6.056, P &lt; 0.05). Stress was the main factor affecting sucrose preference (F = 123.608, P &lt; 0.05). Decreased sucrose preference and suppressed behavior emerged directly following stress, lasting to at least the eighth day after stress in young animals (P &lt; 0.05). The older stress rats showed a lower sucrose preference than young stress rats (P &lt; 0.05). Older control rats behaved differently from the younger control animals in the OF test, spending more time in the central square (P &lt; 0.05), exhibiting fewer vertical movements (P &lt; 0.05) and less grooming (P &lt; 0.05). Following exposure to stress, older-aged rats showed no obvious changes in vertical movement and grooming. This indicates that aged rats were in an unexcited state before the stress period, and responded less to stressful stimuli than younger rats. There was significantly lower BDNF expression in the CA3 and dentate gyrus regions of the hippocampus following stress in both age groups (P &lt; 0.05), a reduction that was still present at the eighth day after stress (P &lt; 0.05). Stress and age were the main factors affecting the expression of BDNF (F = 9.408, P &lt; 0.05; F = 106.303, P &lt; 0.05). The aged stress group showed lower BDNF expression compared to the young stressed group at every testing time point. Conclusion: Stress has age-dependent effects on behavioral responses and hippocampal BDNF expression in rats. 

Retracted: No

PubPeer comments:
 There appears to be a duplication in the histology images (same slide is used for animals ostensibly from different experimental groups). The authors may want to check their data and reach out to the journal.  ,