LncRNA SNHG7 promotes development of breast cancer by regulating microRNA-186
Authors: L Tang
D-G Ji
D-H Sun
X Luo
Y Song
Journal: European review for medical and pharmacological sciences (2018)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: lncRNA; SNHG7; breast cancer; biological functions; mechanism
Affiliations: Department of Breast Surgery, China-Japan Union Hospital, Jilin University, Changchun, China. .

Abstract:  Objective: To elucidate the biological functions of long non-coding RNA (lncRNA) SNHG7 in breast cancer (BC), and its underlying mechanism in the occurrence and progression of BC. Patients and methods: The expression of SNHG7 in 72 pairs of BC tissues and paracancerous tissues was detected by quantitative Real-time polymerase chain reaction (qRT-PCR). Correlation between SNHG7 expressions with pathological indicators of BC patients was analyzed. Similarly, SNHG7 expression in BC cell lines was determined by qRT-PCR as well. After constructing the small inference RNA of SNHG7, cell proliferation, migration and invasion were determined by cell counting kit-8 (CCK-8), 5-Ethynyl-2'-deoxyuridine (EdU) and transwell assay. MicroRNA-186 expression in BC tissues and cells was accessed. Dual-luciferase reporter gene assay was conducted to verify the binding condition between SNHG7 and microRNA-186. Results: SNHG7 expression was higher in BC tissues than that of paracancerous tissues. High expression of SNHG7 was positively correlated to tumor stage, lymph node metastasis and distant metastasis, whereas not correlated to age, sex and tumor location of BC. Kaplan-Meier curves revealed that higher expression of SNHG7 is correlated to the worse prognosis of BC patients. SNHG7 was highly expressed in BC cells as well. Knockdown of SNHG7 inhibited proliferative, invasive and migratory abilities of BC cells. QRT-PCR data showed that microRNA-186 is lowly expressed in BC tissues compared with that of paracancerous tissues. MicroRNA-186 was lowly expressed in BC cells as well. Both mRNA and protein levels of microRNA-186 were negatively correlated to SNHG7 in BC tissues. Finally, the dual-luciferase reporter gene assay demonstrated that SNHG7 could be directly targeted by microRNA-186. Conclusions: SNHG7 is highly expressed in BC, which is correlated to tumor stage, lymph node metastasis and distant metastasis of BC patients. SNHG7 could promote malignant progression of BC by regulating microRNA-186. 

Retracted: No

PubPeer comments:
 The relative colony number including the error bars of pmirGLO-SNHG7, pmirGLO-SNHG7-mut and pmirGLO groups appear similar in MCF-7 and SKBR3. Shown in colored dotted lines. , 
 #1 https: www.europeanreview.org wp wp-content uploads 7788-7797.pdf I found the authors have made Erratum regarding this mistake. I think it might be their carelesness while preparing the figure using the software You did good job !! , 
 *[left]* Fig 4D from [Luo et al (2018)] .  Negative correlation between SNHG7 and microRNA-186 in BC . *[right]* Fig 3E from [Xu et al (2019)] .  MiR-424-5p was significantly negatively correlated with the expression level of ITGB1 in NSCLC tissues. An implausible number of data points are present in both scatterplots.  , 
 Fig 1D.  Survival curves of SNHG7 expression in BC patients.  Another manifestation of Hoya s Law for J.BUOn and ERMPS...  When 40 or 50 cases of any given cancer are dichotomised into half-groups of high- or low-expression of any given nc-RNA, survival rate for one half-group or both will drop to 0% at 75 months. Other examples, from left to right: - Fig 1 from [Zang et al (2020)] - Fig 1 from [Li et al (2020)] - Fig 1, and Fig 4D, from [Cui et al (2019)] - Fig 1D from [Gao et al (2019)] .  *[left]* Figure 1C from [Wu et al (2020)] . *[right]* Figs 1D, 3E from [Wu et al (2019)] .  , 
 *[left]* Fig 4D from [Luo et al (2018)] .  Negative correlation between SNHG7 and microRNA-186 in BC . *[right]* Fig 3C from [Liu et al (2019)] .  A significant negative correlation was found between microRNA-106a expression and DAX-1 expression in BC tissues. Almost all points are the same.  , 
 *[above]* Table 1 from [Luo et al (2018)] . *[below]* Table 1 from [Liu et al (2019)] .  Yes, men can develop breast cancer, but when they comprise almost half the patients in the group, surely this deserves some comment. It is even more noteworthy that two ostensibly-unrelated groups of researchers have reported **almost identical demographics and disease characteristics** for their 72-case samples. , 
 One more paper with a similar correlation plot, also describing men with breast cancer: X.-Y. Niu et al., doi: 10.26355 eurrev_201908_18738, https: pubpeer.com publications B19B8F95B27B0DD55D052B6221DEAD Here is a small collage of the plots. I have highlighted some characteristic data points with colored ellipses.  , 
 Comment deleted; wrong thread ,