Retracted: Micro RNA ‚Ä?1 promotes glioma cell proliferation and inhibits senescence and apoptosis by targeting SPRY 1 via the PTEN / PI 3K/ AKT signaling pathway
Authors: Lai-Jun Song
Chang Chai
Ming Li
Shuang-Yin Han
Xi-Qing Li
Journal: CNS Neuroscience & Therapeutics (2018)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: PTEN/PI3K/AKT signaling pathway; Sprouty1; apoptosis; cell senescence; glioma
Affiliations: Henan Eye Institute, Henan Provincial People's Hospital, Zhengzhou, China., Department of Neurosurgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China., Department of Gastroenterology, Henan Provincial People's Hospital, Zhengzhou, China., Department of Oncology, Henan Provincial People's Hospital, Zhengzhou, China., Department of Neurosurgery, Henan Provincial People's Hospital, Zhengzhou, China.

Abstract:  Aims: Our study aims to investigate the effect of microRNA-21 (miR-21) on the proliferation, senescence, and apoptosis of glioma cells by targeting SPRY1 via the PTEN/PI3K/AKT signaling pathway. Methods: Glioma tissues and brain tissues were collected for this study after surgical decompression for traumatic brain injury. RT-qPCR was employed to measure mRNA levels of miR-21, SPRY1, PTEN, PI3K, and AKT, and Western blotting was conducted to determine protein levels of SPRY1, PTEN, PI3K, AKT, p-AKT, Caspase-3, Caspase-9, P53, GSK3, and p-GSK3. Human glioma U87 cells were assigned into the blank, negative control (NC), miR-21 mimics, miR-21 inhibitors, siRNA-SPRY1, and miR-21 inhibitors + siRNA-SPRY1 groups, with human HEB cells serving as the normal group. Cell proliferation, cell cycle, and apoptosis were determined by MTT and flow cytometry, respectively. Results: Compared with control group, an increased expression of miR-21, PI3K, AKT, p-AKT, P53, and p-GSK3, and a decreased expression of SPRY1, PTEN, Caspase-3, and Caspase-9 were observed in the glioma group, and no significant differences were found in the expression of GSK3. SPRY1 was verified to be the target gene of miR-21. Compared with the blank and NC groups, levels of PI3K, AKT, p-AKT, P53, and p-GSK3 increased while levels of SPRY1, PTEN, Caspase-3, and Caspase-9 decreased in the miR-21 mimics and siRNA-SPRY1 groups; the miR-21 inhibitors group reversed the tendency; furthermore, the miR-21 inhibitors group showed decreased cell proliferation but promoted apoptosis, which were opposite to the results of the miR-21 mimics and siRNA-SPRY1 groups. Conclusion: MicroRNA-21 might promote cell proliferation and inhibit cell senescence and apoptosis of human glioma cells by targeting SPRY1 via the PTEN/PI3K/AKT signaling pathway. 

Retracted: Yes

PubPeer comments:
 Fig 9C.  Flow cytometric detection on the effect of miR-21 on cell cycle distribution and apoptosis among different groups. ... C, cell apoptosis conditions of all groups . Could the authors comment on the clusters of cells showing up in multiple independent experiments?  More similar than I would expect to Fig 11 of [Wu et al (2018)] . No authors in common.  , 
 January 2021 retraction: https: onlinelibrary.wiley.com doi 10.1111 cns.13614  The above article, published online on January 05, 2018, in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between Shuangyin Han and Ming Li, the journal Editoru2010inu2010Chief Jun Chen, and John Wiley Sons Ltd. The retraction has been agreed upon because the article was submitted and approved for publication by Chang Chai without consent in any form by the named cou2010authors Shuangyin Han and Ming Li. , 
 Acknowledgement:  We would like to give our sincere appreciation to the reviewers for their helpful comments on this article. This courteous expression of gratitude seems anodyne, unexceptional, but it appears frequently in products of the  Contractor papermill (along with hand-drawn flow-cytometry scatterplots and cell-cycle analyses). https: docs.google.com spreadsheets d 1r0MyIYpagBc58BRF9c3luWNlCX8VUvUuPyYYXzxWvgY edit?usp=sharing ,