Retracted : Effects of microRNA‚Ä?06 and its target gene IGF‚Ä? on sevoflurane‚Äêinduced activation of hippocampal astrocytes in aged rats through the PI3K/AKT/CREB signaling pathway
Authors: Bin Wang
Qun-Xi Li
Xiao-Liu Dong
Shu-Bo Zhang
Xiao-Liang Han
Tie-Jun Liu
Journal: Journal of Cellular Physiology (2018)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: activation; hippocampal astrocytes; insulin-like growth factor-1; microRNA-206; phosphatidylinositol 3-kinase/protein kinase B/cAMP-responsive element-binding protein signaling pathway
Affiliations: Department of AnesthesiologyNorth China University of Science and Technology Affiliated HospitalTangshanP.R. China, Department of PaediatricsNorth China University of Science and Technology Affiliated HospitalTangshanP.R. China, Department of NeurosurgeryNorth China University of Science and Technology Affiliated HospitalTangshanP.R. China, Department of NeurologyTangshan People's HospitalTangshanP.R. China

Abstract:  The study aims to explore the effects of microRNA-206 (miR-206) targeting IGF-1 on the activation of hippocampal astrocytes in aged rats induced by sevoflurane through the PI3K/AKT/CREB signaling pathway. Wistar rats and astrocytes were divided into the normal/blank, sham/negative control (NC), sevoflurane (sevo), miR-206 mimics+sevo, miR-206 inhibitors+sevo, miR-206 NC+sevo, IGF-1 shRNA+sevo, and miR-206 inhibitors+IGF-1 shRNA+sevo groups. The Morris water maze test was exhibited to assess the cognitive functions. Glial fibrillary acidic protein (GFAP) expression was detected by immunofluorescence assay. Western blotting and RT-qPCR were used to detect the expression of miR-206, IGF-1, PI3K, AKT, CREB, pPI3K, pAKT, pCREB, cytochrome-c (Cyt-c), and caspase-3. Cell viability and apoptosis were detected by MTT assay and annexin V/PI double staining respectively. Mitochondrial transmembrane potential (MTP) were determined by flow cytometry. The IGF-1 shRNA+sevo group showed reduced miR-206 expression. Compared with the normal/blank group, the sevo, and miR-206 NC+sevo groups showed decreased miR-206 and GFAP expressions, cell viability and MTP but increased expressions of IGF-1, PI3K, AKT, CREB, pPI3K, pAKT, pCREB, Cyt-c and caspase-3, as well as cell apoptosis. Similar trends were observed in the miR-206 inhibitors+sevo group when compared with the sevo group. The study provides evidence that miR-206 alleviates the inhibition of activation of hippocampal astrocytes in aged rats induced by sevoflurane by targeting IGT-1 through suppressing the PI3K/AKT/CREB signaling pathway. 

Retracted: Yes

PubPeer comments:
 FIGURE 8  Expressions of IGF-1, pPI3K, pAKT, pCREB, Cyt-c, and caspase-3 in astrocytes among the eight groups. (a) Western blot images of expressions of IGF-1, pPI3K, pAKT, pCREB, Cyt-c, and caspase-3 in astrocytes among each group .  Compare the loading control with Fig. 9A of [Zhou et al (2018)] .  Western blot analysis demonstrated that miR-495 negatively correlated with the protein expression of NLRP3, TNF-u03b1, IL-1u03b2, IL-18 and caspase-1, and the opposite was found for NLRP3. ... (A), western blotting map .  More examples in the Zhou et al thread. , 
 Retracted [30 June 2021] . ------------------------------ The above article, published online on 20 October 2017 in Wiley Online Library has been retracted by agreement between the journal s Editor in Chief, Prof. Dr. Gregg Fields, and Wiley Periodicals LLC. The retraction has been agreed following an investigation based on allegations raised by a third party. Several flaws and inconsistencies between results presented and experimental methods described were found. The editors consider the conclusions of this article to be invalid. ,