Relationship between microRNA-27a and efficacy of neoadjuvant chemotherapy in gastric cancer and its mechanism in gastric cancer cell growth and metastasis
Authors: Chunlei Xu
Na Li
Xushan Tang
Ning Zhou
Hong Cheng
Journal: Bioscience reports (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Cell growth; Efficacy; Gastric cancer; Metastasis; MicroRNA-27a
Affiliations: Department of Digestive Internal Medicine, The Affiliated Tumor Hospital of Xinjiang Medical University, Wunumuqi 830053, P.R. China., Department of Laboratory, The Affiliated Tumor Hospital of Xinjiang Medical University, Wunumuqi 830053, P.R. China., Department of Digestive Internal Medicine, The Affiliated Tumor Hospital of Xinjiang Medical University, Wunumuqi 830053, P.R. China .

Abstract:  Objective: The aim of the present study is to investigate the relationship between microRNA-27a (miR-27a) and the efficacy of neoadjuvant chemotherapy in gastric cancer (GC) and its mechanism in the growth and metastasis of GC cells.Methods: The expression of miR-27a in serum of 74 GC patients received neoadjuvant chemotherapy was detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Clinical value and prognosis of miR-27a expression in predicting the efficacy of neoadjuvant chemotherapy in GC were evaluated. Besides, GC cells with low miR-27a expression were transfected with miR-27a mimics, and cells with high miR-27a expression were transfected with miR-27a inhibitors and secreted frizzled-related protein 1 (SFRP1) siRNA. A series of experiments were applied for the determination of cell viability, invasion and migration of GC cells.Results: After neoadjuvant chemotherapy, the expression of miR-27a in serum of GC patients decreased significantly. Additionally, the expression of miR-27a in GC cell line was significantly higher than that in normal gastric mucosa cell line. Meanwhile, after down-regulating the expression of miR-27a in GC cells, the mRNA and protein expression of SFRP1 increased, the proliferation rate of cells slowed down, and the ability of invasion and migration decreased. Furthermore, combined with low expression of miR-27a and SFRP1, the proliferation rate of GC cells increased and the ability of invasion and migration increased.Conclusion: Collectively, our study highlights that the high expression of miR-27a indicates the poor efficacy and prognosis of neoadjuvant chemotherapy in GC patients. Down-regulation of miR-27a can inhibit the growth and metastasis of GC cells via up-regulation of SFRP1. 

Retracted: No

PubPeer comments:
 Firstly, figures 4, 5, and 7 contain what appear to be numerous image duplications within their scratch assays.    , 
 I m also seeing what appear to be some duplications between the cell line images of figures 5 and 7.   Additionally I see what appears to be at least one in figure 4.  , 
 https: doi.org 10.1042 BSR-20181175_EOC The Editorial Office has been made aware of potential issues surrounding the scientific validity of this paper, hence has issued an expression of concern to notify readers whilst the Editorial Office investigates. , 
 While the Editorial Office continues its investigation, the investigators may care to compare *[right]* Fig 6B:  to Fig 8A of [Song et al (2016)] .  , 
 Retracted [6 August 2021] . -------------------------------- This Retraction follows an Expression of Concern relating to this article previously published by Portland Press. This article is being retracted from Bioscience Reports at the request of the Editor-in-Chief and the Editorial Board following receipt of a notification from a reader, alerting the Editorial Board to duplicated regions and images in Figures 4D, 4E, 4F, 5C, 5D, 5E, 5F, 7C, 7D, 7E and 7F. The authors have been contacted in regards to the Retraction, and have not responded to the Journal s queries and the concerns raised. Given the extent of the issues raised, the Editorial Board stand by the decision to retract the article. ,