Caveolin-1 plays a key role in the oleanolic acid-induced apoptosis of HL-60 cells
Authors: Peng-Xia Zhang
Yu-Kuan Feng
Jin-Hua Piao
Gui-Ming Zhu
Di-Di Wang
Yu Yang
Hong-Mei Gu
Xu Gao
Li Li
Wei Ma
Journal: Oncology Reports (2014)
Discipline: CLINICAL MEDICINE
Keywords: caveolin-1; CAV-1; oleanolic acid; HL-60 cells; apoptosis
Affiliations: School of Basic Medicine, Jiamusi University, Jiamusi 154000, P.R. China., Department of Biology, Mudanjiang Medical University, Mudanjiang 157011, P.R. China., Department of Biochemistry, Harbin Medical University, Harbin 150086, P.R. China.

Abstract:  Our previous study found that caveolin-1 (CAV-1) protein expression is upregulated during oleanolic acid (OA)-induced inhibition of proliferation and promotion of apoptosis in HL-60 cells. CAV-1 is the main structural protein component of caveolae, playing important roles in tumorigenesis and tumor development. It has been shown that cav-1 expression is lower in leukemia cancer cell lines SUP-B15, HL-60, THP-1 and K562 and in chronic lymphocytic leukemia primary (CLP) cells when compared with normal white blood cells, with the lowest cav-1 expression level found in HL-60 cells. To study the effects of cav-1 in HL-60 cells and the effects of cav-1 overexpression on OA drug efficacy, cav-1 was overexpressed in HL-60 cells using lentiviral-mediated transfection combined with OA treatment. The results showed that cav-1 overexpression inhibited HL-60 cell proliferation, promoted apoptosis, arrested the cell cycle in the G1 phase and inhibited activation of the PI3K/AKT/mTOR signaling pathway. Overexpression of CAV-1 also increased HL-60 cell sensitivity to OA. To further verify whether OA affects HL-60 cells via the activation of downstream signaling pathways by CAV-1, cav-1 gene expression was silenced using RNAi, and the cells were treated with OA to examine its efficacy. The results showed that after cav-1 silencing, OA had little effect on cell activity, apoptosis, the cell cycle and phosphorylation of HL-60 cells. This study is the first to show that CAV-1 plays a crucial role in the effects of OA on HL-60 cells. 

Retracted: No

PubPeer comments:
 Figure 4. Multiple flow cytometry images were reused indicated by different color boxes with gating changed.  , 
 Dear editor: Thank you very much.yYour comment is highly appreciated. As you said, we did make some mistakes in the process of drawing, but this does not affect the data analysis and the conclusion of t he article. In fact, in the study, our experimentswere divided into two parts: CAV1overexpression experiment and CAV1silencing experiment. Because there was a period!of time between the two batches of experiments, the voltage setting for the flow cytometry was not completely consistent. Therefore, in the process of analyzing the data, we corrected the gate threshold in order to compare the results from different batches with the same parameters. It s a pity that we made the mistake in reorganizing and selecting maps (as you marked, it is actually the result of the analysis with different parameters of a set of data, such as the threshold of the gatedrift shown in the figure). Sorry again for confusion we have brought to you. In the future, we must pay attention to the problems of correction and post verification in the process of data analysis! ,