von Willebrand factor rescued by miR-24 inhibition facilitates the proliferation and migration of osteosarcoma cells in vitro
Authors: Quanwen Yuan
Lu Zhou
Jing Ling
Zhaohua Bao
Huan Wang
Zhenni Ma
Jie Yin
Xiaofeng Liu
Jun Pan
Ling Liu
Huilin Yang
Yu Zhang
Feng Yuan
Journal: Bioscience reports (2018)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: miR-24; migration; osteosarcoma; proliferation; von Willebrand factor.
Affiliations: Department of Orthopedics, Clinical Medical Research Center of Jiangsu Province, The First Affiliated Hospital of Soochow University, Suzhou 215006, China., Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou 215006, China., Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215006, China., Department of Orthopedics, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221003, China., Department of Orthopedics, Children's Hospital of Soochow University, Suzhou 215003, China., Department of Hematology, Affiliated Hospital of Nantong University, Nantong, 226000 China., Department of Orthopedics, The Friendship Hospital of Ili Kazakh Autonomous Prefecture, Yining 835000, China., Department of Orthopedics, Jiangsu Province Hospital, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China., Department of Orthopedics, Clinical Medical Research Center of Jiangsu Province, The First Affiliated Hospital of Soochow University, Suzhou 215006, China  ., Department of Hematology and Oncology, Children's Hospital of Soochow University, Suzhou 215003, China  .

Abstract:  von Willebrand factor (vWF) is a major procoagulant molecule that was shown to differentiate between metastatic and primary osteosarcoma (OS) tissues and associated with increased metastasis. However, its functional role in OS progression has been unclear yet. The expression profile of vWF and miR-24 in human OS tissues was characterized using immunofluorescence labeling and quantitative real-time PCR analysis. The interaction between miR-24 and vWF was identified by dual luciferase reporter assay. The effects of vWF and miR-24 on OS cells were assessed by cell proliferation, colony formation, and migration. The clinical significance of miR-24 in OS patients was analyzed using Kaplan-Meier analyses and Pearson's Chi-squared test. Here, we reported that the expression of vWF was significantly increased, but miR-24 was significantly decreased in OS tissues (n=84). vWF was further validated as the target of miR-24 in MG-63 and U2OS cells. miR-24 obviously suppressed the proliferation and migration of MG-63 and U2OS cells. However, the migration-inhibiting activity of miR-24 was predominantly attenuated by vWF overexpression. Clinically, low miR-24 expression in human OS tissues was significantly associated with tumor metastasis and predicted a poor survival in OS patients. This work demonstrated that vWF, as a downstream effector of miR-24, played an important role in controlling OS cell progression. Target miR-24 or vWF, therefore, promises to be an effective biological target for OS treatment. 

Retracted: No

PubPeer comments:
 **Computer Vision for Augmentative and Alternative Communication**  Sethuraman Panchanathan, Meredith Moore, Hemanth Venkateswara, Shayok Chakraborty, Troy McDaniel [Arizona State University]  Computer Vision for Assistive Healthcare, 2018, Pages 211-248  https: www.sciencedirect.com science article pii B9780128134450000083 copied approximately a third of the u00a9 2016 IEEE paper: **Person-Centered Multimedia Computing: A New Paradigm Inspired by Assistive and Rehabilitative Applications**  Sethuraman Panchanathan ; Shayok Chakraborty ; Troy McDaniel ; Ramin Tadayon  IEEE MultiMedia ( Volume: 23 , Issue: 3 , July-Sept. 2016)  https: ieeexplore.ieee.org document 7535120 The 2018 version, published by Academic Press Elsevier, did not cite the earlier IEEE version and did not enclose copied passages in quotation marks. The Elsevier version removed the name of author and PhD student Ramin Tadayon while adding authors Meredith Moore and Hemanth Venkateswara. The Elsevier version did not indicate that the copyright holder gave permission for the work to be republished. According to Elsevier: The authors should ensure that they have written entirely original works, and if the authors have used the work and or words of others, that this has been appropriately cited or quoted. ... Proper acknowledgment of the work of others must always be given.  https: www.elsevier.com authors journal-authors policies-and-ethics Reproducing a work word for word, in whole or in part, without permission and acknowledgment of the original source.  Is it unethical? **Yes.**  Literal copying is only acceptable if you reference the source and put quotation marks around the copied text.  https: www.elsevier.com __data assets pdf_file 0009 653886 Plagiarism-factsheet-March-2019.pdf The work was funded by National Science Foundation grants 1069125 ($3 million) and 1116360 ($0.5 million):  https: www.nsf.gov awardsearch showAward?AWD_ID=1069125  https: www.nsf.gov awardsearch showAward?AWD_ID=1116360 ActiveAwards=true ExpiredAwards=true Arizona State University explains that: Whenever you paraphrase or quote someone else s work, you must cite your source in **TWO** places:  1 ) **Within** your paragraph. This is called an u201cin-text citation.u201d Your in-text citation includes brief information a reader will need to find the complete reference in your list of sources such as the author, date or page numbers.  AND  2 ) At the **end** of your paper in a list of sources.  https: www.asu.edu courses asu101 asuonline academic_integrity avoiding_plagiarism_mla_handout.pdf Examples of reproducing the u00a9 2016 IEEE work word for word without permission and acknowledgment of the original source -- which Elsevier characterizes as  unethical -- are shown below, with the Elsevier version on the right.      ,