microRNA-136 inhibits proliferation and promotes apoptosis and radiosensitivity of cervical carcinoma through the NF-Œ∫B pathway by targeting E2F1
Authors: Dong-Mei Wu
Hong-Jie Lu
Jun Lu
Shao-Hua Fan
Zi-Feng Zhang
Fang Wang
Pei-Ying Jin
Yao Tang
Yuan-Lin Zheng
Journal: Life Sciences (2018)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: Apoptosis; Cervical carcinoma; E2F1; NF-Œ∫B signaling pathway; Proliferation
Affiliations: Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou 221116, PR China; College of Health Sciences, Jiangsu Normal University, Xuzhou 221116, PR China., Department of Oncology, Linyi People's Hospital, Linyi, 276000, PR China., Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou 221116, PR China; College of Health Sciences, Jiangsu Normal University, Xuzhou 221116, PR China. Electronic address: .

Abstract:  Aims: Several microRNAs (miRs) are expressed aberrantly and associated with progression, tumorigenesis, and prognosis of haematological and solid tumors. The study aimed to identify the effects involved with microRNA-136 (miR-136) on the concurrent enhancement of proliferation, apoptosis and radiosensitivity of cervical carcinoma through the NF-Œ∫B signaling pathway by targeting E2F1. Main methods: Totally 338 patients with cervical carcinoma were recruited in this study. The expressions of miR-136, E2F1, p65, CyclinD1, Atm, Chk2, Bcl-2, Survivin and Bax were detected using RT-qPCR and Western blot analysis. Cells with highest miR-136 expression were subsequently assigned into different groups. Cell survival and apoptosis rate were detected by colony formation assay and flow cytometry, respectively. Key findings: Compared to the sensitivity group, E2F1, p65, Bcl-2 and Survivin exhibited increased levels, while expression of CyclinD1, Atm, Chk2, Bax and miR-136 was reduced in the confrontation group. Cell survival rate was declined at 6 and 8 Gy of X-ray irradiation compared with 0, 2 and 4 Gy. Compared with the blank and NC groups, expression of E2F1, p65, Bcl-2 and Survivin was increased, while that of CyclinD1, Atm, Chk2, Bax and miR-136 was all decreased. The cell survival rate was increased; while apoptosis rate was decreased in the miR-136 inhibitor group. The trends observed in the miR-136 mimics and siRNA-E2F1 groups were contradictory to the miR-136 inhibitor group. Significance: Based on our results, miR-136 inhibits proliferation, while acting to promote apoptosis and radiosensitivity in cervical carcinoma by targeting E2F1 through the NF-Œ∫B signaling pathway, resulting in improved prognoses. 

Retracted: Yes

PubPeer comments:
 I see some serious problems with the Western immuno blot and PCR data in this publication: Figure 1c: Western immuno blots look strange (blurry). There is one discontinuity in the upper blot, not in the others. Figure 2e: Western immuno blot look strange (blurry) . There is one dicontinuity in the upper blot, not in the others. Figure 3d: Much of the PCR data seem to be duplicated and presented as something else.  , 
 2021 retraction. https: www.nature.com articles s41417-021-00325-5 The Editor-in-Chief has retracted this article because figures 1b, d, 3a, 4b, 5b, d and e, 6a, b, c, as well as parts of the text were duplicated from a previously published article by Bao et al. [1]. In addition, the article shows significant overlap with an article by Tian et al. that was simultaneously under consideration [2] and another previously published article by Wu et al. [3]. The authors have not responded to correspondence regarding this retraction. References 1. Bao Y, Chen Z, Guo Y, Feng Y, Li Z, Han W, et al. Tumor Suppressor MicroRNA-27a in Colorectal Carcinogenesis and Progression by Targeting SGPP1 and Smad2. PLoS ONE. 2014;9:e105991. https: doi.org 10.1371 journal.pone.0105991. 2. Tian K, Di R, Wang L. MicroRNA-23a enhances migration and invasion through PTEN in osteosarcoma. Cancer Gene Ther. 2015;22:351u20139. https: doi.org 10.1038 cgt.2015.27. 3. Wu XL, Cheng B, Li PY, Huang HJ, Zhao Q, Dan ZL, Tian DA, Zhang P. MicroRNA-143 suppresses gastric cancer cell growth and induces apoptosis by targeting COX-2. World J Gastroenterol. 2013;19:7758u201365. ,