miR‚Ä?01 regulates the cell proliferation and apoptosis in diffuse large B‚Äëcell lymphoma by targeting MEK1 via regulation of the ERK/MAPK signaling pathway
Authors: Yiqun Huang
Xudong Ma
Ruiji Zheng
Luhui Lin
Yong Zou
Journal: Oncology Reports (2018)
Discipline: CLINICAL MEDICINE
Keywords: MEK1; miR-101; ERK/MAPK pathway; DLBCL; proliferation
Affiliations: Department of Hematology, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, Fujian 363000, P.R. China.

Abstract:  MAPK kinase 1 (MEK1) is an upstream protein kinase of extracellular signal regulated kinase (ERK), which activates the ERK/MAPK (mitogen activated protein kinase) pathway. Importantly, bioinformatic analysis has shown that there is a target complementary binding site between miR‚Ä?01 and MEK1. The present study aimed to ascertain whether or not miR‚Ä?01 plays a role in regulating MEK1 expression, ERK/MAPK pathway activity, and the proliferation and apoptosis in a diffuse large B cell lymphoma (DLBCL) cell line. DLBCL tumor samples were collected from patients in our hospital, and lymphatic tissues with reactive lymphoid hyperplasia were selected as controls. The patients were divided into high and low expression groups, and then the survival rate of the two groups was compared using Kaplan‚ÄëMeier method, as well the effect of miR‚Ä?01 and MEK1 mRNA expression on survival and prognosis was analyzed. The expression of miR‚Ä?01, MEK1 and p‚ÄëMEK1 between normal lymphoblastic cell lines (HCC1954 BL and NCI‚ÄëBL2009) and lymphoma cell lines (SU‚ÄëDHL‚Ä? and Farage) was compared. Lymphoma SU‚ÄëDHL‚Ä? and Farage cells were cultured in vitro, and then divided into the following groups: miR‚ÄëNC group; miR‚Ä?01 mimic group; siRNA‚ÄëNC group; and siRNA‚ÄëMEK1 group. The expression of miR‚Ä?01, MEK1, p‚ÄëMEK1, p‚ÄëERK1/2 and Bcl‚Ä? was compared. Cell apoptosis was detected by flow cytometry, and cell proliferation was detected by EdU staining. The results showed that targeted regulation existed between miR‚Ä?01 and MEK1, and the decreased expression of miR‚Ä?01 was related to the pathogenesis and prognosis of DLBCL. Upregulation of miR‚Ä?01 inhibited DLBCL cell proliferation and facilitated apoptosis by inhibiting the expression of MEK1. 

Retracted: No

PubPeer comments:
 Chinese bloggers  Medical Coffee Club [report as follows] (thx Google Translate): *On June 8, 2021, the official website of the Department of Science, Technology and Education of the National Health Commission released the u201cResults of Investigation and Handling of a Batch of Medical Scientific Research Integrity Casesu201d. These cases came from public notifications from some medical institutions, including 7 cases involving scientific research.* Case Six is the present paper: *The paper  miR-101 regulates the cell proliferation and apoptosis in diffuse large B-cell lymphoma by targeting MEK1 via regulation of the ERK MAPK signaling pathway was published by Zheng Ruiji as the corresponding author and Huang Yiqun as the first author of Zhangzhou Hospital in Fujian Province. According to the investigation, data deviations and inaccurate graphs in the paper are data fraud .* A [report at the authors institution] provides more details. Indeed, the paper appears to be a product of the [ Paint-it-Black papermill] , described [here] . Fig 3:  Fig 5:  ,