Isorhamnetin inhibited migration and invasion via suppression of Akt/ERK-mediated epithelial-to-mesenchymal transition (EMT) in A549 human non-small-cell lung cancer cells
Authors: Qingbin Liu
Nan Jiang
Li Shi
Wei Luo
Mingquan Li
Journal: Bioscience Reports (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Akt/ERK1/2; Epithelial-mesenchymal transition (EMT); Isorhamnetin; Lung cancer; Metastasis.
Affiliations: Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130021, China., Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130021, China .

Abstract:  In the present study, we investigated the potential effects of Isorhamnetin on the growth and metastasis of A549 human lung cancer cells, as well as the underlying mechanism. Treatment with Isorhamnetin exhibited a dose- and time-dependent inhibition on A549 cell proliferation. Furthermore, the cell adhesion and Transwell assay showed that treatment with Isorhamnetin (2.5, 5, and 10 ŒºM) for 48 h resulted in a significant inhibition effect on cell adhesion, invasion and migration of A549 cells, depending on concentration, which was associated with the suppression of matrix metalloproteinase (MMP)-2 and MMP-9 activity and protein expression. Moreover, Isorhamnetin effectively suppressed the expressions of epithelial-to-mesenchymal transition (EMT) markers, as evidenced by the down-regulation of N-cadherin, vimentin and snail, as well as up-regulation of E-cadherin protein expression. Additionally, these inhibitions were mediated by interrupting AKT/ERK1/2 signaling pathways. Taken together, the results of the current study demonstrated that Isorhamnetin may become a good anti-metastastic agent against lung cancer A549 cell line by the suppression of EMT via interrupting Akt/ERK1/2 signaling pathway. 

Retracted: No

PubPeer comments:
 Figure 5D appears to have the same image used to represent two different treatments, with a horizontal flip and different contrast (which makes certain features more less prominent. *** Original  *** Flipped  , 
 I am intrigued by Figs 3B and 3C, migration invasion assays where the membranes contain a mixture of vertical pores (circles) and diagonal pores (caterpillar-like elongations). The horizontal and vertical discontinuties are particularly intriguing. Here, three frames from 3B, with some of the discontinuities marked at the right.    One from 3C:  Did Fig 3 fall into the paper shredder, so it had to be reassembled with sellotape? Some close-ups:  , 
 The Editorial Office has been made aware of potential issues surrounding the scientific validity of this paper, hence has issued an expression of concern to notify readers whilst the Editorial Office investigates. , 
 https: doi.org 10.1042 BSR-20190159_COR This Correction follows an Expression of Concern relating to this article previously published by Portland Press. The authors of the original paper u201cIsorhamnetin inhibited migration and invasion via suppression of Akt ERK-mediated epithelial-to-mesenchymal transition (EMT) in A549 human non-small cell lung cancer cellsu201d (Biosci Rep (2019) 39(9), DOI: 10.1042 BSR20190159) would like to replace Figures 3 and 5 with a new set of images, due to an unintentional mistake when preparing the submission. In an attempt to adjust the images in Figure 3B,C to be consistent with Figure 3A-C had become discontinuous and blurry in appearance. The wrong image was uploaded in the final revision before publication for Figure 5, instead of the one that was originally submitted upon the second revision of the manuscript (this can be verified via the submission system records). The corrections made in this erratum will not alter the conclusion of the results. The authors apologize for any inconvenience or misunderstanding that these errors may have caused. The corrected Figures 3 and 5 are updated as shown in this correction article. ***  ***  , 
 #4 Many thanks to the authors for the explanation of the artefact in the image. This explanation, in combination with the publication history, has resolved my concerns. ,