MicroRNA-206 facilitates gastric cancer cell apoptosis and suppresses cisplatin resistance by targeting MAPK2 signaling pathway
Authors: D-Y Ding
D-F Song
Y Feng
Z Chen
Y-J Gao
R-Z Hou
D-Y Wang
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: MAPK3; miR-206; gastric cancer; proliferation; apoptosis
Affiliations: Department of Nephrology, First Hospital of Jilin University, Changchun, Jilin, China. .

Abstract:  Objective: Mitogen activating protein kinase 3 (MAPK3) is critical in extracellular signal-regulated kinase (ERK)/MAPK pathway. Gastric cancer tissues have microRNA-206 (miR-206) down-regulation. This study aimed to investigate the role of miR-206 in MAPK3 expression, gastric cancer cell proliferation, apoptosis, and cisplatin (DDP) resistance. Materials and methods: Dual-Luciferase reporter gene assay confirmed targeted regulation between miR-206 and MAPK3. DDP resistant cell line BGC823/DDP and SGC7901/DDP were generated for comparing miR-206 and MAPK3 expression against parental cells using quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blot, followed by flow cytometry measuring apoptosis. Drug-resistant cells were transfected with miR-206 mimic for measuring MAPK3 and phosphorylated MAPK3 (p-MAPK3) expression. Flow cytometry and EdU were employed for measuring cell apoptosis and proliferation. Results: Targeted regulation existed between miR-206 and MAPK3 mRNA. BGC823/DDP and SGC7901/DDP cell presented lower miR-206 than parental cells, plus higher MAPK3 mRNA or protein. Under DDP treatment equivalent to IC50 of parental cells, drug-resistant cells presented lower apoptosis compared to parental drug-sensitive cells. Compared to miR-normal control (miR-NC) group, miR-206 mimic transfection significantly decreased MAPK3 and p-MAPK3 protein expression (p &lt; 0.05), enhanced cell apoptosis and weakened proliferation potency (p &lt; 0.05). Conclusions: The down-regulation of miR-206 is associated with DDP resistance of gastric cancer cells. Up-regulating miR-2016 expression can weaken the proliferation of drug-resistant gastric cancer cells, facilitate cell apoptosis and decrease DDP resistance via targeted inhibition of MAPK3 expression. 

Retracted: No

PubPeer comments:
 This paper has features suggestive of [what has elsewhere been labeled as  Papermill #2 ] , characterised by stylised geometrical WBs that are reduced to black ovals on a featureless grey background, reminiscent of a Miro painting. Here softer-focus than in most manifestations.  The other characteristic is a small repertoire of recurring flow cytometry plots,   slightly modified by artisanal stippling on each of their occurrences.  Other examples are listed in a Google document. https: docs.google.com spreadsheets d 1qBqQMXgzxoBd5bTbqdMuapGheupE5bdIopypju417NM edit?usp=sharing , 
 China s Ministry of Science Technology weighed in, in [a report from May 2022] ,  Summary of the public notification of the results of the investigation and handling of medical research integrity cases in some universities (May 23, 2022) . This is Case 23. Thx Google Translate. 23. The paper  MicroRNA-206 facilitates gastric cancer cell apoptosis and suppresses cisplatin resistance by targeting MAPK2 signaling pathway was written by Feng Ye from the China-Japan Friendship Hospital of Jilin University as the corresponding author and Chen Zhi from the First Hospital of Jilin University as the first author. After investigation, it was found that the paper fabricated the research process and falsified the research data and charts. Chen Zhi will be dealt with as follows: notice of criticism, disqualification of science and technology planning projects (special projects, funds, etc.) For one year, Feng Ye (combined with another paper) will be dealt with as follows: notice of criticism, disqualification of scientific and technological planning projects (special projects, funds, etc.) for 6 years, and cancellation of various awards The eligibility for evaluation of excellence is 2 years, the valid application qualification for job promotion and professional title promotion will be canceled once, the post allowance will be withheld for 24 months, and the postgraduate enrollment qualification review will be prohibited for 2 years. -------------------------- The editors of ERMPS continue to stand by the paper s validity. ,