Effect of miR-181a-3p on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by targeting BMP10
Authors: GuiLu Tao
Ping Mao
CunDi Zhong
MinFei Jiang
JiaZheng Liu
Tongbin Chu
HaoNan Guan
Journal: Artificial Cells Nanomedicine and Biotechnology (2019)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: BMP10; MCSs; miR-181a-3p; osteogenic differentiation.
Affiliations: Department of Wound Repairment, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China;, Department of Laboratory, Second Affiliated Hospital of Dalian Medical University, Dalian, China;, Department of Wound Repair, Second Affiliated Hospital of Dalian Medical University, Dalian, China

Abstract:  Objective: To explore the regulation relationship between miR-181a-3p and BMP10, and their mechanism of osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs).Methods: After osteogenic induction of MSCs, the ALP activity was detected by ELISA. The expression of miRNA-181a-3p and BMP10 was detected by RT-qPCR, and the protein levels of BMP10 and osteogenic differentiation marker proteins ALK and RUNX2 were detected by Western blot. The TargetScan online website was used to predict the putative target of miR-181a-3p, and dual luciferase reporter assay was performed to validate the targeting relationship between miR-181a-3p and BMP10.Results: In osteogenic differentiation of MSCs, ALP activity, the level of ALK and RUNX2 was evidently increased (p &lt; .05), and the expression of miR-181a-3p was significantly downregulated (p &lt; .05). Moreover, overexpression of miR-181a-3p obviously decreased the expression of BMP10 (p &lt; .05), miR-181a-3p knockdown increased the expression of BMP10 prominently (p &lt; .05). The transfection of miR-181a-3p mimics resulted in significantly downregulation of ALP activity and RUNX2 protein expression in MSCs (p &lt; .05). In addition, overexpression of BMP10 could reverse the inhibitory effect of miR-181a-3p on osteogenic differentiation (p &lt; .05).Conclusions: In conclusion, we found that miR-181a-3p inhibited osteogenic differentiation of MCSs by targeting BMP10. 

Retracted: Yes

PubPeer comments:
 The text of this encyclopedia entry overlaps in large part with that of the book Oskar Peterlini, *Instruments for Direct Democracy in Italy* (Prokopp Hechensteiner 2012) ISBN 9788860690135, available at [www.researchgate.net publication 316701649_Instruments_for_Direct_Democracy_in_Italy_Strumenti_di_democrazia_diretta_in_Italia](http: www.researchgate.net publication 316701649_Instruments_for_Direct_Democracy_in_Italy_Strumenti_di_democrazia_diretta_in_Italia), pages 49u201372 and 85u201386. Example, Ratto Trabucco (2018) page 2 on the left, Peterlini (2012) page 49 on the right:  Total extent of overlap, Ratto Trabucco (2018) on the left and Peterlini (2012) extract pages 49u201372 and 85u201386 on the right:  Picture generated in Sofia Kalaidopoulou s *similarity texter*, [people.f4.htw-berlin.de ~weberwu simtexter app.html](http: people.f4.htw-berlin.de ~weberwu simtexter app.html): pdf converted to txt line breaks replaced by spaces double spaces replaced by single spaces copied and pasted into similarity texter and run with minimum match length 4 zoomed out and screenshot quality rendered down. Non-readability here is intentional so as not to infringe copyright. This encyclopedia entry has also been published, with a few revisions, as a journal article: Fabio Ratto Trabucco, The Advantages and Disadvantages of Italian Referendum Tools (2019) 24(1) Biau0142ostockie Studia Prawnicze 151, [doi.org 10.15290 bsp.2019.24.01.11](http: doi.org 10.15290 bsp.2019.24.01.11). ,