LINC00968 accelerates the progression of epithelial ovarian cancer via mediating the cell cycle progression
Authors: N Yao
B-Q Guo
J-Q Sun
L Yu
L Ma
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: LINC00968; epithelial ovarian cancer; cell cycle progression; cell proliferation; tumor xenograft
Affiliations: Department of Pathology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China. .

Abstract:  Objective: The aim of this study was to clarify the potential role of LINC00968 in the progression of epithelial ovarian cancer (EOC) and the underlying mechanism. Patients and methods: The relative expression level of LINC00968 in EOC tissues (n=40) and normal ovarian tissues (n=40) was determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). LINC00968 expression in human-derived ovarian cancer cell lines was examined by qRT-PCR as well. After transfection of LINC00968 small-interfering RNA (siRNA) in ovarian cancer cells, cell cycle progression and cell proliferation were evaluated through flow cytometry, Cell Counting Kit-8 (CCK-8) and colony formation assay, respectively. Tumor xenograft was conducted in nude mice to elucidate the function of LINC00968 in EOC tumorigenesis in vivo. Furthermore, the relative expression levels of cell cycle factors and protein kinase B/extracellular-signal-regulated kinase (AKT/ERK) in ovarian cancer cells influenced by LINC00968 were detected by Western blot. Results: LINC00968 was significantly up-regulated in EOC tissues when compared with normal control tissues. Meanwhile, LINC00968 expression was positively correlated with the prognosis of EOC. Transfection of LINC00968 siRNA in HEY and HO8910 cells markedly attenuated proliferative ability and arrested cell cycle in the G1 phase. Knockdown of LINC00968 remarkably suppressed tumor growth of EOC in nude mice. The silence of LINC00968 significantly downregulated Cyclin D, Cyclin E and CDK4, whereas upregulated p16 and p21. In addition, AKT and ERK pathways were inhibited by knockdown of LINC00968 in ovarian cancer cells. Conclusions: LINC00968 expression is markedly upregulated in EOC. Meanwhile, it arrests the cell cycle in the G1 phase by inhibiting the ERK and AKT pathways, thus accelerating EOC progression. 

Retracted: No

PubPeer comments:
 Fig 2E, F.  This is an example of what has elsewhere been labeled as  Papermill #2 , characterised by stylised WBs reminiscent of the blobs in a Miro painting,  ...and by a small repertoire of recurring FACS plots, slightly modified by artisanal stippling on each of their occurrences.  https: forbetterscience.com 2020 02 27 dark-satanic-papermills , 
 Retracted [6 August 2021] . ---------------------- *Cancer Biotherapy and Radiopharmaceuticals* officially retracts the paper entitled, u201cmiR-155 Affects Osteosarcoma MG-63 Cell Autophagy Induced by Adriamycin Through Regulating PTEN-I3K AKT mTOR Signaling Pathway Cancer Biotherapy and Radiopharmaceuticals,u201d by Lin Wang, Bing Tang, Heng Han, Dan Mao, Jie Chen, Yun Zeng, and Min Xiong (Cancer Biother Radiopharm 2018;33(1):32u201338; doi: 10.1089 cbr.2017.2306) due to the discovery that the paper was submitted from a paper mill, which is a violation of the journal s standard protocols and is considered an infraction against the rigorous standards of scientific publishing. The Editor and Publisher of *Cancer Biotherapy and Radiopharmaceuticals* are committed to preserving the scientific literature and the community it serves and does not tolerate any violations of scientific misconduct. , 
 http: www.most.gov.cn zxgz kycxjs kycxgzdt 202201 t20220127_179206.html Google Translated Date: January 27, 2022 14:44 Source: Ministry of Science and Technology u3000u300041. The paper  miR-155 Affects Osteosarcoma MG-63 Cell Autophagy Induced by Adriamycin Through Regulating PTEN-PI3K AKT mTOR Signaling Pathway in which Xiong Min is the corresponding author and Wang Lin is the first author. After investigation, it was found that the paper had academic misconduct such as purchasing experimental data, writing and investing on behalf of others. Wang Lin will be dealt with as follows: withdraw the manuscript, return the issued paper awards, cancel the application qualifications for scientific research projects, talent projects, and scientific and technological awards at all levels and various levels for 5 years, cancel the qualification for merit evaluation for 5 years, and cancel the qualification for applying for professional titles for 5 years. , revoke the scientific research projects that use the thesis conclusion, and deduct the quarterly performance; make the following treatment for Xiong Min: admonishing the conversation, suspending the qualification for evaluation and evaluation for one year, and canceling the qualifications for application of various scientific research projects, talent projects, and scientific and technological awards at all levels 1 year; other authors, Tang Bing, Han Heng, Mao Dan, Chen Jie, Zeng Yun, will be dealt with as follows: admonishment to talk, cancel the application qualifications for various scientific research projects, talent projects, and scientific and technological awards at all levels for 1 year. u3000u3000(Notification link: https: www.dfmhp.com.cn Html News Articles 3073.html) ,