Down-Regulated LncRNA-HOTAIR Suppressed Colorectal Cancer Cell Proliferation, Invasion, and Migration by Mediating p21
Authors: Jie-Ting Zheng
Yi Jiang
Ling-Ling Zhang
Shu-Yao Zhang
Yu-Xian Yang
Guo-Dong Qiu
Chen Chen
Kai Lin
Ling Fang
Yu-Qi She
Hong Jiang
Journal: Digestive Diseases and Sciences (2018)
Discipline: CLINICAL MEDICINE
Keywords: Colorectal cancer; HOX transcript antisense RNA; Invasion; Migration; Proliferation
Affiliations: Family Medicine Centre, The First Affiliated Hospital of Shantou University Medical College, Shantou, 515041, People's Republic of China., Department of Radiology, Cancer Hospital of Shantou University Medical College, Shantou, 515031, People's Republic of China., Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College, Shantou, 515041, People's Republic of China., Medical Oncology, Cancer Hospital of Shantou University Medical College, Shantou, 515031, People's Republic of China., Department of Pharmacy, Cancer Hospital of Shantou University Medical College, No. 7, Raoping Road, Shantou, 515031, Guangdong Province, People's Republic of China., Clinical Pharmacy Research Center, Shantou University Medical College, No. 22, Xinling Road, Shantou, 515041, Guangdong Province, People's Republic of China., Department of Pharmacy, Cancer Hospital of Shantou University Medical College, No. 7, Raoping Road, Shantou, 515031, Guangdong Province, People's Republic of China. ., Clinical Pharmacy Research Center, Shantou University Medical College, No. 22, Xinling Road, Shantou, 515041, Guangdong Province, People's Republic of China. .

Abstract:  Background and aim: HOX transcript antisense intergenic RNA (HOTAIR) is a relatively well-understood RNA, which plays a central role in the pathogenesis of various tumors. The aim of the present study was to investigate the effect by which HOTAIR acts to influence the biological processes of colorectal cancer (CRC) through p21. Methods: Reverse transcription quantitative polymerase chain reaction and Western blot methods were employed to provide verification regarding the changes in HOTAIR, PCNA, Ki67, p21, cyclin E, and CDK2 among the CRC tissues and cells. The correlation between the clinicopathological characteristics of patients and expression of HOTAIR and p21 was subsequently evaluated, followed by an analysis into the effects of HOTAIR on the biological processes of M5 cells. Results: HOTAIR was found to be expressed at high levels, while p21 was determined to be at a low level among both the CRC tissues and the CRC cell lines. The expressions of HOTAIR and p21 were determined to be related to lymph node metastasis, tumor node metastasis, Dukes staging, distant metastases, histological types, and the degree of differentiation. Cells transfected with HOTAIR siRNA displayed inhibited rates of proliferation, invasion, and migration, as well as decreased cyclin E and CDK2, while apoptosis and p21 were increased. Conclusion: The principal findings demonstrated that down-regulation of HOTAIR elicits an inhibitory effect on proliferation, invasion, and migration, while promoting the apoptosis of CRC cells through the up-regulation of p21. We believe that HOTAIR could represent a novel target for the treatment of CRC. 

Retracted: No

PubPeer comments:
 Fig 6(b,e).  b represents the Western blot analysis for expression of cyclin E, CDK2, and p21 in each group... e represents the Western blot analysis for expression of PCNA and Ki67 in each group .  The protein blobs are familiar, especially the bubble under one of the blobs in the 6(e) control band. Previous sightings include Figs 2A,B and 3 of [Chu et al (2018)] .   ,