LncRNA XIST promotes migration of Wilms' tumor cells through modulation of microRNA-193a-5p
Authors: Y Hou
R He
Y-C Jiang
D Qian
F-Z Yao
Journal: European review for medical and pharmacological sciences (2020)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: lncRNA XIST; Wilms' tumor; clinical features; prognosis; qRT-PCR
Affiliations: Department of Pediatrics, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, China. .

Abstract:  Objective: The aim of this study was to investigate long non-coding RNA (lncRNA) XIST expression in Wilms' tumor (WT) and to further explore its relationship with clinical features and prognosis of WT patients. Patients and methods: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was carried out to examine the expression level of XIST in tumor tissue samples and paracancerous ones collected from 43 patients with renal cell carcinoma, and the interplay between XIST expression and clinical indicators, as well as prognosis of patients was analyzed. Meanwhile, XIST level in the nephroblast cancer cell line was further confirmed by qRT-PCR. In addition, XIST knockdown model was constructed using lentivirus in the WT cell lines, including HFWT and 17-94, and the influence of XIST on WT cell functions was analyzed through transwell assay. Finally, we investigated whether lncRNA XIST plays a role in the progression of WT by modulating microRNA-193a-5p. Results: In this research, qRT-PCR results revealed a significantly higher expression of lncRNA XIST in tumor tissue specimens of patients with renal cell carcinoma than that in adjacent ones. Compared with patients with low expression of lncRNA XIST, those with high XIST expression had a higher incidence of distant metastasis and a lower overall survival rate. Compared with the negative control group, the metastatic ability of WT cells in the lncRNA XIST knockdown group was markedly weakened. In addition, the results of qPCR showed that mRNA expression of lncRNA XIST and microRNA-193a-5p were negatively correlated in renal cell carcinoma tissue specimens. At the same time, silencing microRNA-193a-5p reversed the reduced metastasis ability of WT cells induced by knockdown of XIST. Conclusions: LncRNA XIST expression is dramatically enhanced in WT tissues and cell lines, which is closely associated with the incidence of distant metastasis and patients' poor prognosis. In addition, we demonstrated that lncRNA XIST may accelerate the malignant progression of WT via inhibiting microRNA-193a-5p. 

Retracted: No

PubPeer comments:
 Fig 3D.  There was a significant negative correlation between the expression of lncRNA XIST and miR-193a-5p in WT tissues . Rescaled for comparison with (right) a superimposition of 14 versions of the same scatterplot, in different productions from a papermill. Darker data-points appeared more frequently across the variants.  , 
 Another characteristic feature of this papermill is pairs of crenellated K-M plots, resembling combs or walls of Lego blocks, with survival declining precipitously at 75 months. Here, Figs 1C and 1F.  Kaplan Meier survival curve of patients with renal cell carcinoma based on lncRNA XIST expression was shown; the prognosis of patients with high expression of lncRNA XIST was significantly worse than those in low expression group ...  Kaplan Meier survival curve of patients with renal cell carcinoma based on miR-193a-5p expression was shown; the prognosis of patients with low expression of miR-193a-5p was significantly worse than those in high expression group .  ,