FH535 inhibits proliferation and migration of colorectal cancer cells by regulating CyclinA2 and Claudin1 gene expression
Authors: Longjin Jin
Dan Hong
Keke Wang
Yiyan Jiang
Yiwen Jiang
Zhefeng Lou
Xuezi Tu
Journal: Gene (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Cancer therapy; Claudin1; Colorectal cancer; CyclinA2; FH535.
Affiliations: Wenzhou Medical University School of Laboratory Medicine and Life Sciences, Wenzhou 325000, China., Wenzhou Medical University First Affiliated Hospital, Wenzhou 325000, China., Wenzhou Medical University School of Laboratory Medicine and Life Sciences, Wenzhou 325000, China. Electronic address: .

Abstract:  Wnt signaling pathway plays a major role in the progression of colorectal cancer (CRC). Small molecules which can cut off this signal transduction can be promising anti-cancer drugs for CRC therapy. Therefore, we aimed to investigate the mechanisms of FH535, an inhibitor of the Wnt signaling pathway, on inhibiting proliferation and migration of colorectal cancer cells DLD-1 and SW620. We found that FH535 could significantly suppress the growth of DLD-1 and SW620 cells in a concentration-dependent and time-dependent manner. The results of cell cycle tests showed that FH535 could significantly induce G2/M arrest in colorectal cancer cells. Transwell and Wound-healing assays revealed that FH535 notably inhibited cell migration. Moreover, we found that FH535 down-regulated Œ≤-catenin and CyclinA2 expressions while up-regulating Claudin-1 expression at both mRNA and protein levels, which may contribute to the FH535-induced inhibitory effect on proliferation and migration in human colorectal cancer cells. Our study revealed that FH535 inhibited proliferation and migration of colorectal cancer cells by regulating CyclinA2 and Claudin1 gene expression, which enriches regulatory network of FH535 and may contribute to being promising anti-cancer drugs for CRC therapy. 

Retracted: No

PubPeer comments:
 Figure 2C appears to have an image which overlaps. TAM. ***  , 
 We appreciated the revieweru2019s attention to detail, and we are sorry that we did not find out such a big mistake during the proof. Now we have checked the raw data and revised the image of Figure 2C. The related original results, statistical results and corresponding to Figure 2C were in the appendix. Thanku2002youu2002again foru2002youru2002valuableu2002andu2002thoughtfulu2002comments. We are sorry that we failed to upload the image, please find the raw data and new figures image in https: figshare.com s dc2b3c1bf3117b2b5048, 10.6084 m9.figshare.14820444. Thank you again! , 
 Figure 2D ***  , 
 Some of the graphs in Figure 1 appear more similar than expected. Could the authors check and perhaps provide higher resolution images? One example below. ***  , 
 After self-inspection of the article, we found problems with image duplication for the field of view was repeated when we took pictures as well as some similar pictures are repeated in the raw data. And we are sorry that we made such big mistakes unintentionally. And we have uploaded our original data inu00a0https: doi.org 10.6084 m9.figshare.14906250.v1. Sorry about the inconvenience caused. ,