Therapeutic approach for global myocardial injury using bone marrow-derived mesenchymal stem cells by cardiac support device in rats
Authors: Muhammad Naveed
Muhammad Saeed
Qin Zhang
Hafiz Muhammad Zubair
Cuican Li
Zhou Xiaohui
Reyaj Mikrani
Ziwei Liu
Mirza Muhammad Faran Ashraf Baig
Muhammad Asim Farooq
Journal: Biomedical Microdevices (2021)
Discipline: CLINICAL MEDICINE
Keywords: Active cardiac support device (ASD); BMSCs; Epicardial delivery; Global myocardial injury; Stem cell treatment.
Affiliations: Department of Clinical Pharmacy, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, School of Pharmacy, Nanjing, Jiangsu Province, 211198, People's Republic of China., State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China., Faculty of Animal Production and Technology, The Cholistan University of Veterinary and Animal Sciences, Bahawalpur, 6300, Pakistan., Department of Pharmacy, School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu Province, 211198, People's Republic of China., Faculty of Pharmacy, University of Lahore, Lahore, Punjab, Pakistan., Department of Clinical Pharmacy, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, School of Pharmacy, Nanjing, Jiangsu Province, 211198, People's Republic of China. .

Abstract:  Bone marrow-derived mesenchymal stem cells (BMSCs) have been considered a promising therapeutic approach to cardiovascular disease. This study intends to compare the effect of BMSCs through a standard active cardiac support device (ASD) and intravenous injection on global myocardial injury induced by isoproterenol. BMSCs were cultured in vitro, and the transplanted cells were labeled with a fluorescent dye CM-Dil. Isoproterenol (ISO) was injected into the rats; 2 weeks later, the labeled cells were transplanted into ISO-induced heart-jury rats through the tail vein or ASD device for 5 days. The rats were sacrificed on the first day, the third day, and the fifth day after transplantation to observe the distribution of cells in the myocardium by fluorescence microscopy. The hemodynamic indexes of the left ventricle were measured before sacrificing. H&amp;E staining and Masson's trichrome staining were used to evaluate the cardiac histopathology. In the ASD groups, after 3 days of transplantation, there were a large number of BMSCs on the epicardial surface, and after 5 days of transplantation, BMSCs were widely distributed in the ventricular muscle. But in the intravenous injection group, there were no labeled-BMSCs distributed. In the ASD + BMSCs-three days treated group and ASD + BMSCs -five days-treated group, left ventricular systolic pressure (LVSP), the maximum rate of left ventricular pressure rise (+dP/dt), the maximum rate of left ventricular pressure decline (-dP/dt) increased compared with model group and intravenous injection group (P &lt; 0.05). By giving BMSCs through ASD device, cells can rapidly and widely distribute in the myocardium and significantly improve heart function. 

Retracted: No

PubPeer comments:
 Figure 2. * Cyan boxes: Panels d2 (BMSCs) and e1 (Labeled cells after passage) appear to show the same specimen, albeit shown at different exposure, and perhaps with some of the bright cells seen in d2 missing in e1.  Here is a close up of the two panels, with a slightly better drawn box around the regions that look remarkably similar.  ,