<p>C-terminal of E1A binding protein 1 enhances the migration of gastric epithelial cells and has a clinicopathologic significance in human gastric carcinoma</p>
Authors: Zhaocheng Chi
Hongyu Wang
Min Wang
Bocheng Xing
Chunxiao Lie
Can Wang
Han Dong
Journal: OncoTargets and Therapy (2019)
Discipline: CLINICAL MEDICINE
Keywords: C-terminus of the E1A binding proteins; Janus Kinase 1; gastric cancer; signal transducer and activator of transcription 3.
Affiliations: Second Department of Gastrointestinal Surgery, Jilin Provincial Cancer Hospital, Changchun, Jilin 130012, People's Republic of China., Department of Pathology, Jilin Provincial Cancer Hospital, Changchun 130012, People's Republic of China., Internal Medicine of Abdominal Tumors, Jilin Provincial Cancer Hospital, Changchun 130012, People's Republic of China., Department of Geriatric Medicine, First Hospital of Jilin University, Changchun, Jilin 130012, People's Republic of China.

Abstract:  Background: Recent studies have claimed that the C-terminal of E1A binding proteins (CtBPs) influence tumorigenesis through participating in cell signal transduction in various human tumors. However, the detailed expression profiles of CtBP isoforms in human gastric cancer (GC) and the molecular mechanisms of CtBP involvement in tumor cell phenotypes warrant further investigation. Materials and methods: The expression of CtBPs in GC cell lines and a human gastric epithelial cell line were explored via RT-qPCR and Western blotting assays. Moreover, the expression profiles of CtBPs in GC and histologically noncancerous tissues were explored by immunohistochemistry. To explore the effects of CtBP1 on the metastatic phenotype in GC, gastric epithelial cells were transfected with a eukaryotic expression plasmid to overexpress CTBP1, and the endogenous CtBP1 or JAK1 in GC cells was silenced through an RNA interference (RNAi) method. These transfections were validated via Western blotting, and the activation state of the JAK1/Stat3 signaling pathway was also explored via Western blotting. Furthermore, the malignant phenotype of GC cells was evaluated via a Cell Counting Kit-8 (CCK8) assay, colony formation assay, transwell assay, and wound-healing experiment. Results: Our data revealed that the expression of CtBP1, but not CTBP2, was upregulated in 102 GC tissue samples compared with 98 noncancerous tissue samples, and the elevated expression level of CtBP1 was notably associated with distant metastasis. CTBP1 modulated cell migration and invasion through the JAK1/Stat3 signaling pathway in gastric epithelial cells. In addition, genetic silence of CtBP1 expression in GC cells notably constrained cell proliferation, invasion and migration abilities through inhibiting the activation of the JAK1/Stat3 pathway in GC cells. Conclusion: Our data reveal that the knockout of CtBP1 notably constrains distant metastasis in GC through the JAK1/Stat3 pathway, suggesting that targeting CtBP1 is a practical anti-tumor approach to restrain tumor progression in GC. 

Retracted: Yes

PubPeer comments:
 Retracted [10 March 2021] . --------------------------- The Editor and Publisher of OncoTargets and Therapy wish to retract the published article. It came to our attention that images in Figure 4E had allegedly been duplicated, specifically: - Figure 4E panel Scramble 0h appears to be duplicated with Figure 4E panels CTBP1-RNAi and JAK1-RNAi 0h, with all images showing evidence of frame shifting. - Figure 4E panel CTBP1-RNAi 12h appears to be duplicated with Figure 4E JAK1-RNAi 24h. The authors were unable to provide a satisfactory explanation for the alleged duplication nor were they able to provide the raw data for their study. The Editor determined the findings of the study are no longer valid and requested for the paper to be retracted. Our decision-making was informed by our policy on publishing ethics and integrity and the COPE guidelines on retraction. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as u201cRetractedu201d. --------------------  ,