Up-regulation of microRNA-497-5p inhibits colorectal cancer cell proliferation and invasion via targeting PTPN3
Authors: Sen Hong
Helei Wang
Zhenkun Yan
Lei Ding
Miaomiao Bi
Journal: Bioscience reports (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Colorectal cancer (CRC); invasion; microRNA-497-5p (miR-497-5p); proliferation; protein tyrosine phosphatase non-receptor type 3 (PTPN3).
Affiliations: Department of Colorectal and Anal Surgery, The First Hospital of Jilin University, Changchun 130021, Jilin, China., Department of Endoscopy Center, China-Japan Union Hospital of Jilin University, Changchun 130022, Jilin China., Department of Gastrointestinal Surgery, The First Hospital of Jilin University, Changchun 130021, Jilin, China  ., Department of Radiology, China-Japan Union Hospital of Jilin University, Changchun 130022, Jilin, China  ., Department of Ophthalmology, China-Japan Union Hospital of Jilin University, Changchun 130022, Jilin, China.

Abstract:  To investigate the role of microRNA-497-5p (miR-497-5p) in the tumorigenesis of colorectal cancer (CRC), the present study applied qRT-PCR to detect the expression level of miR-497-5p in both clinical samples and CRC cell lines. Furthermore, to specifically evaluate the carcinogenic role of miR-497-5p in CRC, the expression of miR-497-5p was monitored by transfecting with the mimics or inhibitors of miR-497-5p. Transwell assay as well as CCK-8 assay were used to determine the functions of miR-497-5p on cell invasion, migration and proliferation, respectively. miR-497-5p expression was remarkably down-regulated in clinical samples with cancer development as well as in CRC cell lines. Additionally, low miR-497-5p expression was remarkably correlated with higher TNM stage and lymph node metastasis of CRC patients. Up-regulation of miR-497-5p significantly inhibited proliferation, migration, and invasion of LOVO CRC cell line. Conversely, antagonizing miR-497-5p significantly promoted cell proliferation, migration and invasion. Mechanistic analysis revealed that miR-497-5p directly bound to its downstream target, protein tyrosine phosphatase non-receptor type 3 (PTPN3), whose aberrant expression partially reversed inhibition of cell proliferation and migration. Taken together, the present study elucidated the inhibitory role of miR-497-5p in CRC via targeting PTPN3, which potentiated miR-497-5p as a potential therapeutic target for combating CRC. 

Retracted: No

PubPeer comments:
 Figure 4D shows common elements shared across all 4 images. Some indicated with like-colored ellipses. Cyan arrows indicates an area of difference within an area of overlap. ***  *** Forensically clone analysis  *** Full figure  , 
 Fig 1B,  Pathological assay of representative CRC and adjacent matched-carcinoma tissues using H E staining .   I have concerns. , 
 Fig 1D, E are weird diagrams. They are not swarm plots, for there is no system to the horizontal jitter; nor are they strip plots, which do not taper at top and bottom.  More to the point, the superimposed I-beams (means and standard deviations? Medians and quartiles?) bear no discernible relationship to the corresponding clouds of points. , 
 Fig 4C. Zooming in on one frame at random...  A lot of work went into it.  , 
 #1 Note areas of cancellation when a color-reversed version of miR-497-5p is superimposed on the Control.  Non-cancellation areas are mainly due to extra groups of cells present in the Control slide. , 
 #5 Knowing which clumps of cells are present in the Control slide but not in the corresponding areas of the mIR-497-5p slide makes it easier to find them in pCDNA + Scramble.  , 
 #4 Agreed. An impressive amount of work went into making these images.  , 
 The Editorial Office has been made aware of potential issues surrounding the scientific validity of this paper, hence has issued an expression of concern to notify readers whilst the Editorial Office investigates. , 
 https: doi.org 10.1042 BSR-20191123_RET Retracted: This Retraction follows an Expression of Concern relating to this article previously published by Portland Press. This article is being retracted from Bioscience Reports at the request of the Editor in Chief and Editorial Board following receipt of a notification from several readers, alerting the Editorial Office to manipulation in Figures 1B, 2C, 4C, and 4D. The authors have been contacted in regard to the retraction, and have not responded to the Journal. Given the extent of the issues raised, Editorial Board stand by the decision to retract the article. ,