A new Cu(II)-containing coordination polymer: protective effect and mechanism exploration on elderly osteoporotic hip fractures via regulating the wnt signaling pathway
Authors: Bingqin Ma
Shangding Xie
Jie Yang
Liangzhi Wang
Kai Yu
Journal: Molecular Diversity (2021)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: Coordination polymer; Molecular docking; Osteoporotic hip fractures; RT-PCR.
Affiliations: Department of Orthopedics, Tianjin Fifth Central Hospital, Tianjin, China., Department of Orthopedics, Tianjin Fifth Central Hospital, Tianjin, China. ., Department of Medicine, Soochow University, Changzhou, Jiangsu, China.

Abstract:  Elderly osteoporosis hip fracture has drawn the attention of the researcher. The coordination polymer was now widely used in clinic because their multiple structures and biological activities. In this present research, the new coordination polymer was designed and synthesized and their application values on elderly osteoporosis hip were evaluated. The reaction between Cu(II) salt and 4,4'-(1H-1,2,4-triazol-1-yl)methylene-bis(benzoic acid) (H2tzmb), an aromatase inhibitor letrozole derivative with the aid of the organic linker 4,4-bipyridine (4,4'-bpy) affords a new coordination polymer based on Cu(II) ions as nodes of {[Cu2(tzmb)2(4,4'-bpy) (Œº2-H2O)2(DMA) (H2O)2]¬∑10DMA}n (1). The complex 1 gained is totally investigated with the powder X-ray diffraction study, thermogravimetric analyses, the diffraction of single-crystal X-ray, elemental analysis as well as the Fourier transform infrared spectrometer spectra. The osteogenic differentiation of mesenchymal stem cell was measured with the western blotting assay through determining the Runx2 expression. The wnt signaling pathway relative expression in mesenchymal stem cell was detected through the determination of real-time RT-PCR. The cytotoxicity of the compound on the mesenchymal stem cell was determined with CCK-8 assay. The result of single-crystal X-ray diffraction reflects that the complex 1 has crystallized in space group R-3c of trigonal system and exhibits a three-dimensional skeleton architecture on the bases of the SBUs {Cu(tzmb)(Œº2-H2O)}n. The western blotting assay results revealed that this compound could significantly promote the osteogenic differentiation of mesenchymal stem cell. Besides, the activation of wnt signaling pathway in mesenchymal stem cell was also increased by the compound exposure. Finally, it can be summed up that this compound has excellent application values on the elderly osteoporotic hip fractures treatment by increasing the activation of wnt signaling pathway in mesenchymal stem cell. The results of the CCK-8 assay indicated that the compound has no cytotoxicity on the mesenchymal stem cell. The molecular docking simulation results have identified that only the carboxyl group on the Cu complex exhibits the activity for the hydrogen bond formation, however, the pyridine ring does not have such activity, instead, the pyridine ring only acts as the ligand that binds to the Cu ion. This Cu(II) coordination polymer has excellent application values on the elderly osteoporotic hip fractures treatment by increasing the activation of wnt signaling pathway in mesenchymal stem cell. 

Retracted: No

PubPeer comments:
  Therefore, the choice of biocompatible, safe, along with efficient ligands is the major factor in clinical application, the design of structure, as well as the drug therapy [11u201314].  - [11]. Zhao J, Yuan Q, Sun Y, Zhang J, Zhang D, Bian R (2021) Effect of fluoxetine on enhanced biological phosphorus removal using a sequencing batch reactor. Bioresource Technol 320:124396 Really? --------------  All the **preformation** was approved by the Animal Ethics Committee of China (approval number: SCXK2020-0013, approval time: 2020.02) ...  In brief, the mesenchymal stem cells in the **logical growth phage** were collected and seeded into the 96 well plates at the **final destiny** of 10^5 cells per well . There are some peculiar words and terms here.  Logical growth phage , along with  preformation in this present sense of  performance , occurs only in [the products of a prolific coordination-polymer papermill] . , 
 Fig 5 shows results for a control substance, presumably added at the same concentrations as the trial compound.  The authors forgot details of the control when they wrote the text. Could they provide them now? Statistical comparisons and a non-significant result are meaningless without any statement of what s being compared.  This preformation was conducted totally under the guidance of the instructions with only a little modification. In brief, the mesenchymal stem cells in the logical growth phage were collected and seeded into the 96 well plates at the final destiny of 10^5 cells per well. All the cells were cultured in an incubator at the condition of 37 u00b0C, 5% CO2 for 12 h. Then, the compound was added into the wells with serial different dilutions (1u201380 u03bcM). , 
  All the preformation was approved by the **Animal Ethics Committee of China** (approval number: SCXK2020-0013, approval time: 2020.02) Are the authors certain that such an institution exists? ,