p300 promotes proliferation, migration, and invasion via inducing epithelial-mesenchymal transition in non-small cell lung cancer cells
Authors: Li Zhang
Likun Chen
Xi Chen
Wenfeng Fang
Yuanyuan Zhao
Ting Zhou
Haoxian Yang
Yuxiang Ma
Fan Luo
Xue Hou
Run Gong
Yaxiong Zhang
Yunpeng Yang
Shaodong Hong
Jianhua Zhan
Gang Chen
Fangfang Gao
Shuxiang Ma
Yan Huang
Zhonghan Zhang
Journal: BMC Cancer (2018)
Discipline: CLINICAL MEDICINE
Keywords: Epithelial-mesenchymal transition; Invasion; Non-small cell lung cancer; Prognosis; p300.
Affiliations: Department of Medical Oncology, Sun Yat-sen University Cancer Center, 651 East Dongfeng Road, 510060, Guangzhou City, Guangdong Province, People's Republic of China., State Key Laboratory of Oncology in South China, Guangzhou City, Guangdong Province, People's Republic of China., Collaborative Innovation Center for Cancer Medicine, Guangzhou City, Guangdong Province, People's Republic of China., Department of Medical Oncology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai City, Guangdong, People's Republic of China., Department of Clinical Research, Sun Yat-sen University Cancer Center, Guangzhou City, Guangdong Province, People's Republic of China., State Key Laboratory of Oncology in South China, Guangzhou City, Guangdong Province, People's Republic of China. ., Collaborative Innovation Center for Cancer Medicine, Guangzhou City, Guangdong Province, People's Republic of China. ., Department of Thoracic Oncology, Sun Yat-sen University Cancer Center, 651 East Dongfeng Road, 637300, Guangzhou City, Guangdong, People's Republic of China. ., Department of Medical Oncology, Sun Yat-sen University Cancer Center, 651 East Dongfeng Road, 510060, Guangzhou City, Guangdong Province, People's Republic of China. .

Abstract:  Background: Histone acetyltransferase p300 is a crucial transcriptional coactivator and has been implicated as a poor prognostic factor in human cancers. However, little is known about the substantial functions and mechanisms of p300 in NSCLC proliferation and distant metastasis. Methods: We constructed p300 down-regulated and up-regulated cell lines through RNAi and recombinant plasmid transfection. Cell Counting Kit-8 assays were used to test the cell proliferation and confirmed by colony formation assays. Wound healing assays and transwell chamber assays were used to test the migration and invasion ability. Based upon these results, we measured the epithelial markers and mesenchymal markers after regulating p300 expression to explore epithelial-mesenchymal transition as a potential mechanism of p300 promoting NSCLC metastasis. Results: In NSCLC cells NCI-H1975 and NCI-H1993, down-regulation of p300 leads to inhibition of cell proliferation and colony formation. Cells with reduced p300 expression also demonstrate inhibited migration and invasion ability. Contrarily, up-regulation of p300 significantly enhanced the proliferation, colony formation, migration and invasion ability of NCI-H460. Importantly, further investigation shows that decreased p300 expression is associated with reduced expression of mesenchymal markers and increased expression of epithelial markers, while up-regulated p300 expression correlated with decreased expression of epithelial markers and increased expression of mesenchymal markers. Conclusions: As a crucial tumor promoter, p300 promotes cell proliferation, migration, and invasion in NSCLC cells. Epithelial-mesenchymal transition is a potential mechanism of p300 promoting NSCLC metastasis. 

Retracted: No

PubPeer comments:
 Figure 2D appears to have a repeated image, although rotated 90u00b0. Compare H1975 shNC and H1993 shNC. ***  , 
 Update from the journal: u201dDear Reader, Thank you for your email and interest. The case is still under in-house investigation.u201d , 
 09 February 2021 Editoru2019s Note: Readers are alerted that concerns have been raised about Figure 2D. Appropriate editorial action will be taken once this matter is resolved. ,