Resveratrol down-regulates endothelin type B receptors in vascular smooth muscle cells via Sirt1/ERK1/2/NF-–∫B signaling pathways
Authors: Ting Wang
Bingqiao Huang
Linting Wei
Rongguo Fu
Sheping Chen
Wanggang Zhang
Journal: European Journal of Pharmacology (2018)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: Endothelin type B receptor; Oxidized low density lipoprotein; Resveratrol; Sirt1; Superior mesenteric artery.
Affiliations: Department of Haematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China., Department of Haematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China. Electronic address: ., Department of Kidney, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China., Department of Kidney, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China. Electronic address: .

Abstract:  Silent information regulator family protein 1 (Sirt1) has gained attention for protective effects against cardiovasc diseases. Vascular smooth muscle endothelin type B (ETB) receptors are related to the pathogenesis of cardiovascular diseases. Elevated oxidized low-density lipoprotein (ox-LDL) is associated with atherosclerosis. This study will investigate whether resveratrol (a Sirt1 activator, Res) is involved in oxidized low density lipoprotein (ox-LDL)-mediated- regulation of ETB receptors in rat superior mesenteric arteries (SMA). The rat SMA segments were cultured in the presence and absence of ox-LDL with or without Res and specific inhibitor (U0126) for the extracellular signal-regulated protein kinase 1 and 2 (ERK1/2) for 24 h. After organ culture, the contractile responses to sarafotoxin 6c (S6c) were studied using a sensitive myograph, and the ETB receptor protein expression was detected using Western blotting. The results showed that Res concentration-dependently suppressed the ox-LDL -induced up-regulation of ETB receptors expression and receptor-mediated vasoconstriction. In addition, these effects could be inhibited by U0126. Furthermore, activity of ERK1/2 phosphorylation and P65 acetylation induced by ox-LDL were blocked by Res. In conclusion, Res down-regulated ETB receptors through up-regulating Sirt1 and followed by ERK1/2/NF-–∫B signaling pathways in the organ culture SMA. 

Retracted: No

PubPeer comments:
 (a) In eq.(2) of Definition 2, the term $lambda$ is missing in the term $$(1-beta) S_R(alpha)$$ The index defined by eq.(2) is used in **Step 5 of Algorithm II** for ranking alternatives. The numerical results based on eq.(2) in Example 1 are hence not reliable.   (b) The notion of consistency defined by eq.(8) in Definition 6 is not invariant to the permutation of alternatives and hence meaningless. To be clear, let the preference relation on ${x_1, x_2, x_3}$ be $$(1,1,1)quad (1,2, 3)quad (1, 4, 9)$$ $$(1 3,1 2,1)quad (1,1, 1)quad (1, 2, 3)$$ $$(1 9,1 4,1)quad (1 3,1 2, 1)quad (1, 1, 1)$$ It is consistent according to eq.(8) since $$(1,2,3)times (1, 2, 3)=(1, 4, 9)$$ Now, let $$x_1 to y_2 qquad x_2 to y_3 qquad x_3to y_1$$ The preference relation becomes $$(1,1,1)quad (1 9,1 4,1) quad (1 3,1 2,1)$$ $$(1, 4, 9)quad (1,1, 1)quad (1, 2, 3)$$ $$(1,2, 3) quad (1 3,1 2, 1)quad (1, 1, 1)$$ It is not consistent according to eq.(8) since $$(1 9, 1 4, 1)times (1,2, 3)  eq (1 3, 1 2, 1)$$  , 
 For point a), it is a typo and does not effect the main contribution of this paper. Eq.(2) in Definition 1 is cited from other scholars to ranking the triangular fuzzy numbers. We use it in the final step of Algorithm II. Sorry for making you confused because of our carelessness. Thanks for your comments. For point b), please see the following links: https: pubpeer.com publications 02302428068CCE89CE7718D7E30881 https: pubpeer.com publications B40D58EDD22ACE96D6A143A80B144A ,