Calcium plus vitamin D supplementation influences biomarkers of inflammation and oxidative stress in overweight and vitamin D-deficient women with polycystic ovary syndrome: a randomized double-blind placebo-controlled clinical trial
Authors: Khadijeh Nasri
Mehri Jamilian
Ahmad Esmaillzadeh
Zatollah Asemi
Teibeh Hashemi
Rezvan Talaee
Naeeme Talaee
Fereshteh Bahmani
Fatemeh Foroozanfard
Journal: Clinical Endocrinology (2015)
Discipline: CLINICAL MEDICINE
Keywords: calcium; vitamin D; supplementation; inflammatory factors; biomarkers
Affiliations: Department of Gynecology and Obstetrics; School of Medicine; Kashan University of Medical Sciences; Kashan Iran, Department of Gynecology and Obstetrics; School of Medicine; Arak University of Medical Sciences; Arak Iran, Research Center for Biochemistry and Nutrition in Metabolic Diseases; Kashan University of Medical Sciences; Kashan Iran, Department of Dermatology; School of Medicine; Kashan University of Medical Sciences; Kashan Iran, Food Security Research Center; Isfahan University of Medical Sciences; Isfahan Iran

Abstract:  Objective: This study was conducted to determine the effects of calcium plus vitamin D supplementation on inflammatory factors and biomarkers of oxidative stress among overweight vitamin D-deficient women with polycystic ovary syndrome (PCOS). Design, patients and measurements: This randomized double-blind placebo-controlled clinical trial was performed among 104 overweight vitamin D-deficient women diagnosed with PCOS aged 18-40 years. Participants were randomly divided into four groups. Group A received 1000 mg calcium daily and vitamin D placebo weekly (N = 26), group B 50000 IU vitamin D weekly and calcium placebo daily (N = 26), group C 1000 mg calcium daily plus 50000 IU vitamin D weekly (N = 26) and group D calcium placebo daily plus vitamin D placebo weekly (N = 26) for 8 weeks. Fasting blood samples were taken at baseline and 8 weeks after intervention to measure inflammatory factors and biomarkers of oxidative stress. Results: After 8 weeks, individuals taking calcium plus vitamin D supplements had greater decreases in homoeostatic model assessment beta-cell function (HOMA-B) score (-11¬∑1 vs -8¬∑6, -3¬∑4 and 13¬∑7, respectively, P = 0¬∑03), serum high-sensitivity C-reactive protein (hs-CRP) (-948¬∑3 vs 802¬∑3, -383¬∑8 and 618¬∑2 ng/ml, respectively, P = 0¬∑04) and plasma malondialdehyde (MDA) concentrations (-0¬∑6 vs -0¬∑5, -0¬∑1 and 0¬∑6 Œºmol/l, respectively, P = 0¬∑009), and significant increases in plasma total antioxidant capacity (TAC) (35¬∑2 vs 21¬∑1, 22¬∑5 and -153¬∑8 mmol/l, respectively, P = 0¬∑006) and glutathione (GSH) levels (216¬∑0 vs 3¬∑9, -47¬∑5 and -160¬∑8 Œºmol/l, respectively, P = 0¬∑001) compared with calcium alone, vitamin D alone and placebo groups. Calcium plus vitamin D cosupplementation did not influence plasma NO and catalase levels. Conclusions: We found that calcium plus vitamin D cosupplementation for 8 weeks among overweight and vitamin D-deficient women with PCOS had beneficial effects on inflammatory factor and biomarkers of oxidative stress. 

Retracted: No

PubPeer comments:
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 #1 The blots were analyzed using Licor secondary antibodies with different channels (p105 p50 on red and tubulin on green) and the WB for both p105 p50 and tubulin were done at the same time (since they are read on different channels). While the tubulin and p50 bands are distinct, the reason the bands look similar is likely because p50 and tubulin run at around the same Mol. Weight. ,