An efficient protocol to generate placental chorionic plate-derived mesenchymal stem cells with superior proliferative and immunomodulatory properties
Authors: Chen Luo
Ruohong Liu
Shuai Li
Qilin Huang
Lijun Tang
Tao Chen
Yi Wen
Hongyu Sun
Yi Yang
Journal: Stem Cell Research & Therapy (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Chorionic plate-derived MSCs; Isolation; Macrophage; Mesenchymal stem cells; The modified explant culture method.
Affiliations: Department of General Surgery & Pancreatic Injury and Repair Key Laboratory of Sichuan Province, The General Hospital of Western Theater Command (Chengdu Military General Hospital), Chengdu, 610083, China., College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China., Department of General Surgery & Pancreatic Injury and Repair Key Laboratory of Sichuan Province, The General Hospital of Western Theater Command (Chengdu Military General Hospital), Chengdu, 610083, China. ., College of Medicine, Southwest Jiaotong University, Chengdu, 610031, China. .

Abstract:  Background: Placenta-derived MSCs (P-MSCs) represent a promising tool for cell-based therapeutic applications. However, the increasing demand for P-MSCs in clinical trials makes high quality and large number of P-MSCs mandatory. Here, we aim to develop an efficient protocol for P-MSC isolation and culture. Methods: The modified explant culture (MEC) method by combining an initial mild enzymatic reaction with the subsequent explant culture was developed to simultaneously produce various P-MSCs from the different regions of the placenta in serum-free medium (SFM). Its isolation efficiencies, cell yield, and proliferative capacity were compared with the conventional explant culture (EC) method. Furthermore, we determined whether functional properties of P-MSCs are affected by the used tissue-harvesting sites in terms of their proliferation, migration, and the immunomodulatory effect on macrophage. Results: The MEC method achieved higher yield and shorter time in primary cell confluence in SFM compared with the conventional method. The harvested cells possessed the MSC characteristics and demonstrated significantly stronger proliferation ability. Importantly, MSCs derived from chorionic plate (CP-MSCs) were found to exhibit superior properties to the other P-MSCs in proliferation and migration capacity, maintaining the fetal origin over serial passages. Notably, CP-MSCs show stronger ability in regulating macrophage polarization from M1 to M2. Conclusion: Our study developed an efficient and high-yield technique to produce high-quality P-MSCs from the placenta, hence serving as an optimal source of MSCs for clinical application. 

Retracted: No

PubPeer comments:
 Two images in figure 3B appear to overlap. Scale bars seem to be the same although magnification and color differ. *** Contrast enhanced  ***  , 
 We thank Dr. Actinopolyspora Biskrensis so much for pointing out the mistake in Figure 3B. We do deepest apologize to all readers for the mistake we made. In our article, Figure 3B shows adipogenic differentiation of MSCs. Sadly, as you said, the two images of the adipogenic differentiation of CP-MSCS and UC-MSCs do overlap in some areas in our published article. To clarify this mistake, we checked out our original data carefully, and we found that when taking photos of the adipogenic differentiation results of UC-MSCs, multiple areas were taken at the same time, and some of the pictures had overlapping areas. However, during the image saving process, we mistakenly saved the image of UC-MSCs adipogenic differentiation into the data folder of CP-MSCs adipogenic differentiation. Moreover, the background colors of the two pictures are different, and only partially overlapped, so that we found no errors in the picture layout and proofreading. We have corrected the Figure 3B in the following figure, we also provided the initial images for this experiment in the end. We are contacting the editor office of Stem Cell Research Therapy for this correction. This correction does not change any conclusions made in the article. We are really appreciated for your comment. Finally, we do deepest apologize to all readers for the misunderstanding owing to our carelessness.   ,