Polyacrylonitrile / graphene oxide nanofibers for packed sorbent microextraction of drugs and their metabolites from human plasma samples
Authors: Mohammadreza Hadjmohammadi
Mohamed Abdel-Rehim
Abdusalam Uheida
Mohammad Mahdi Moein
Hanieh Karimiyan
Journal: Talanta (2019)
Discipline: CHEMISTRY
Keywords: Human plasma; LC-MS/MS; Local anesthetics; Microextraction by packed sorbent; Nanofibers
Affiliations: Functional Materials Division, Department of Applied Physics, School of Engineering Sciences, KTH Royal Institute of Technology, Isafjordsgatan 22, Kista, SE-164 40 Stockholm, Sweden; Department of Chemistry, University of Mazandaran, Babolsar, 47416-95447, Iran., Functional Materials Division, Department of Applied Physics, School of Engineering Sciences, KTH Royal Institute of Technology, Isafjordsgatan 22, Kista, SE-164 40 Stockholm, Sweden., Department of Chemistry, University of Mazandaran, Babolsar, 47416-95447, Iran., Department of Clinical Neuroscience, Centre for Psychiatry Research, Karolinska Institutet, SE-171 76 Solna, Sweden., Functional Materials Division, Department of Applied Physics, School of Engineering Sciences, KTH Royal Institute of Technology, Isafjordsgatan 22, Kista, SE-164 40 Stockholm, Sweden; Department of Clinical Neuroscience, Centre for Psychiatry Research, Karolinska Institutet, SE-171 76 Solna, Sweden. Electronic address: .

Abstract:  In the present study, a new graphene based nanofibers material (Polyacrylonitrile/Graphene Oxide (PAN/GO)) was used for microextraction by packed sorbent (MEPS). The PAN/GO nanofiber was synthesized using the electrospinning technique. MEPS online with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was utilized for the extraction and determination of two local anesthetic drugs (lidocaine, prilocaine) and their major metabolites (2,6-xylidine, o-toluidine) in human plasma samples. Parameters affecting the extraction efficiency were investigated and optimized (including sample pH, washing solution and elution solution). The validation of the method was based on FDA (Food and Drug Administration) guidelines for bioanalytical methods. The calibration curve ranged from 2.00 to 2000 nmol/L for lidocaine and prilocaine, and from 10.0 to 2000 nmol/L for 2,6-xylidine and o-toluidine. The coefficient of determination (R2) values were 0.996, 0.995, 0.995, 0.996 (n = 3) for lidocaine, prilocaine, 2,6-xylidine and o-toluidine, respectively. The extraction recovery was 93.0% for lidocaine, 96.0% for prilocaine, 68.0% for 2,6-xylidine and 69.0% for o-toluidine. The limits of detection (LODs) were 0.25, 0.50, 2.50, 1.25 nmol/L for lidocaine, prilocaine, 2,6-xylidine and o-toluidine, respectively. The lower limits of quantification (LLOQs) were 2.0 nmol/L for lidocaine and prilocaine, and 10 nmol/L for 2,6-xylidine and o-toluidine, respectively. The accuracy values for the quality control (QC) samples were in the range of 91.0-111% for lidocaine, 92.0-118% for prilocaine, 84.0-98.0% for 2,6-xylidine and 82.0-90.0% for o-toluidine. The inter-day precisions for QC samples ranged from 7.0% to 11.8% for lidocaine, from 8.6% to 11.7% for prilocaine, from 8.0% to 10.0% for 2,6-xylidine and from 8.0% to 9.0% for o-toluidine. The matrix effect values were in the range of -2.3% to -8.6% for lidocaine, -2.7% to -10.2% for prilocaine, 4.8%-5.2% for 2, 6-xylidine and -8.2% to 9.4% for o-toluidine. 

Retracted: No

PubPeer comments:
 Figure 1(a) appears strikingly similar to an image which has been published previously. See https: pubpeer.com publications 15B3F7256EEF1BAE771FD1F295C7DC https: pubpeer.com publications 21128F647815F0FC550AD22595EA7C ***  ***  ,