Effect of Yi Guan Jian decoction on differentiation of bone marrow mesenchymalstem cells into hepatocyte-like cells in dimethylnitrosamine-induced liver cirrhosis in mice
Authors: Yan Xiang
Ying Zhu
Qiao-Ling Xie
Long-Qing Lu
Yuan Zhang
Ai-Jing Leng
Bing-Yao Pang
Hai-Long Chen
Journal: Molecular Medicine Reports (2017)
Discipline: CLINICAL MEDICINE
Keywords: Yi Guan Jian decoction; bone marrow mesenchymal stem cells; hepatocyte-like cells; liver cirrhosis; dimethylnitrosamine
Affiliations: Department of Infectious Diseases, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China., Department of Pharmacy, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China., Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.

Abstract:  Yi Guan Jian decoction (YGD) may induce the differentiation of bone marrow mesenchymal stem cells (BMSCs) into hepatocyte-like cells (HLCs); however, the underlying mechanisms remain to be elucidated. The present study aimed to investigate this process. To do this, a dimethylnitrosamine (DMN)-induced liver cirrhosis mouse model was established. The mice from the model group were randomly divided into three subgroups: i) Negative control, ii) hepatocyte growth factor and iii) YGD. The overall health, liver function and histological alterations were monitored. The expression of Œ±‚Äësmooth muscle actin (Œ±‚ÄëSMA), C‚ÄëX‚ÄëC chemokine receptor type 4 (CXCR4), extracellular signal‚Äëregulated kinase (ERK1/2), nuclear factor Œ∫B p65 subunit (NF‚ÄëŒ∫B p65) and Œ≤‚Äëcatenin were measured by immunohistochemistry, western blotting and reverse transcription‚Äëquantitative polymerase chain reaction. Following administration of DMN, the overall health of the mice significantly decreased, with an increase in pathological developments and liver damage resulting in a decrease in liver function. Immunohistochemistry revealed that the expression of Œ±‚ÄëSMA, CXCR4, ERK1/2, NF‚ÄëŒ∫B p65 and Œ≤‚Äëcatenin was upregulated. Following treatment with YGD, the overall health, liver function and pathology improved. The mRNA and protein expression levels of CXCR4 and ERK1/2 were upregulated, where as Œ±‚ÄëSMA, NF‚ÄëŒ∫B p65 and Œ≤‚Äëcatenin levels were downregulated. The results demonstrated that YGD may induce the differentiation of BMSCs into HLCs to reverse DMN‚Äëinduced liver cirrhosis; this may be achieved via an upregulation of the SDF‚Ä?/CXCR4 axis to activate the mitogen activated protein kinase/ERK1/2 signaling pathway. 

Retracted: No

PubPeer comments:
 Figure 1A and 1B seem a bit confused. Overlaps roughly indicated, although those marked with magenta have been flipped. I may have missed some, and I did not check elsewhere in this paper. ***  , 
 #1 While some of these repeated areas might be explained, there are many others in the paper which seem unlikely to be expected. Figure 5: ***  ***  , 
 Published online on: September 17, 2021 Following the publication of the above article, an interested reader to the authors attention that there appeared to be several duplications of data panels featured within Figs. 1u20113. After having consulted their original data, the authors have realized that a number of the data panels were inadvertently assembled incorrectly in these figures. The corrected versions of Fig. 1A (showing the correct data for the NCu20112W and NCu20114W experiments), Fig. 1B (including the correct data for the Cu20114W, Mu20112W, NCu20112W and NCu20114W experiments), Fig. 2 (showing the correct data for the YGDu20112W experiment), Fig. 3A (NCu20113W data panel corrected), Fig. 3B (HGFu20111W and NCu20113W data panels corrected) and Fig. 3C (Cu20114W data panel corrected) are shown on the next four pages. All these corrections were approved by all authors. The authors regret that these errors were not resolved before the publication of the paper, thank the Editor of Molecular Medicine Reports for granting them the opportunity to publish this corrigendum, and apologize to the readership for any inconvenience caused. [the original article was published in Molecular Medicine Reports 15: 613u2011626, 2017; DOI: 10.3892 mmr.2016.6083].    , 
 Unfortunately, the correction does not appear to resolve the image issues. Corrected Figure 1A seems to have two images which overlap, one after 180-degree rotation. Shown with enhanced sharpness to aid the reader. ***  , 
 Corrected Figure 1B may have similar issues. ***  , 
 Corrected Figure 3B as well. ***  ,