Doxorubicin-loaded poly (lactic-co-glycolic acid) nanoparticles coated with chitosan/alginate by layer by layer technology for antitumor applications
Authors: Jieli Li
Linlin Sun
Liang Ge
Thomas J Webster
Yuanfen Liu
Fei Xiong
Fujuan Chai
Chunli Zheng
Xinyi He
Journal: International journal of nanomedicine (2017)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: burst release; chitosan/alginate; layer by layer; multilayer growth influencing factors; pH-dependent
Affiliations: Department of Pharmaceutics, China Pharmaceutical University, Nanjing., Wenzhou Institute of Biomaterials and Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China., Department of Chemical Engineering, Northeastern University, Boston, MA, USA., Department of Pharmacy, Jiangsu Jiankang Vocational College., State Key Laboratory of Bioelectronics, Jiangsu Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, People's Republic of China.

Abstract:  Natural polyelectrolyte multilayers of chitosan (CHI) and alginate (ALG) were alternately deposited on doxorubicin (DOX)-loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) with layer by layer self-assembly to control drug release for antitumor activity. Numerous factors which influenced the multilayer growth on nano-colloidal particles were studied: polyelectrolyte concentration, NaCl concentration and temperature. Then the growth regime of the CHI/ALG multilayers was elucidated. The coated NPs were characterized by transmission electron microscopy, atomic force microscopy, X-ray diffraction and a zeta potential analyzer. In vitro studies demonstrated an undesirable initial burst release of DOX-loaded PLGA NPs (DOX-PLGA NPs), which was relieved from 55.12% to 5.78% through the use of the layer by layer technique. The release of DOX increased more than 40% as the pH of media decreased from 7.4 to 5.0. More importantly, DOX-PLGA (CHI/ALG)3 NPs had superior in vivo tumor inhibition rates at 83.17% and decreased toxicity, compared with DOX-PLGA NPs and DOX in solution. Thus, the presently formulated PLGA-polyelectrolyte NPs have strong potential applications for numerous controlled anticancer drug release applications. 

Retracted: No

PubPeer comments:
 Figure 6. Panels A and B appear to show some repetitive areas. Boxes of the same color have been drawn around areas that look similar. The nanoparticles in panel A do not show any structure, but appear to look like blank holes in the background. Could the authors provide an explanation of this unexpected appearance in both panels, please?  , 
 June 2021 Erratum found here: https: www.dovepress.com corrigendum-doxorubicin-loaded-poly-lactic-co-glycolic-acid-nanopartic-peer-reviewed-fulltext-article-IJN  The authors have advised that the wrong images for Figure 6 on page 1798 were used. The correct Figure 6 is shown below. The authors wish to apologize for this error and advise that this does not change the conclusions of the paper. In contrast to COPE guidelines, I was not informed of this decision. I not only posted my concerns here, I also wrote to the Editor in Chief. Of other note, one of the senior authors on this paper was the Editor in Chief of this journal. Here is the corrected Figure - with some stains not present in the original, published, figure.  ,