MicroRNA-182 suppresses clear cell renal cell carcinoma migration and invasion by targeting IGF1R
Authors: L Cui
H Li
X Wang
Q Fan
J Feng
Journal: Neoplasma (2016)
Discipline: CLINICAL MEDICINE
Keywords: MicroRNA-182; clear cell renal cell carcinoma; insulin-like growth factor 1 receptor.; migration and invasion.

Abstract:  The purpose of our study was aimed to determine the functional role of microRNA (miR)-182 in clear cell renal cell carcinoma (ccRCC) and try to clarify its underlying molecular mechanism. Expression of miR-182 in both cancer and peripheral blood samples was analyzed by quantitative real-time PCR (qRT-PCR). Human RCC line Caki-1 cells were transfected with miR-182 mimic, miR-182 inhibitor, or negative controls, and then the cell viability, colony-formation ability, migration, and invasion assay were determined. Luciferase reporter assay, qRT-PCR and Western blotting were used to determine whether insulin-like growth factor 1 receptor (IGF1R) was a target of miR-182. Further, small interfering RNA (siRNA) against IGF1R was co-transfected with miR-182 inhibitor into cells, and then the effects on migration and invasion were assessed. MiR-182 was down-regulated in both cancer and blood samples compared to the matched non-tumor adjacent tissues and healthy volunteers, respectively (both P&lt;0.05). Compared to the control group, cell viability, colony-forming ability, and numbers of migrated and invaded cells were significantly decreased by transfection with miR-182 mimic but were markedly increased by miR-182 inhibitor (all P &lt; 0.05). Luciferase reporter assay confirmed that IGF1R was a target gene of miR-182, and IGF1R was negatively regulated by miR-182. Co-transfection of miR-182 inhibitor with si-IGF1R reversed the effect of miR-182 inhibitor on the migration and invasion of the cells. MiR-182 functions as an anti-oncogene in ccRCC, and miR-182-mediated inhibition of cell migration and invasion might be through directly targeting IGF1R. 

Retracted: No

PubPeer comments:
 This paper belongs to a set of over 400 papers (as per February 2020) that share very similar Western blots with tadpole-like shaped bands, the same background pattern, and striking similarities in title structures, paper layout, bar graph design, and - in a subset - flow cytometry panels. Despite these similarities, these papers are authored by researchers from different departments and institutes, with almost no overlap in authors. The list of these papers is available here: https: docs.google.com spreadsheets d 1KXqTAyl4j-jVorFPMD2XRpr76LcIKJ0CVyIvRj0exYQ edit?usp=sharing A more detailed description of this set of papers, suspected to all be generated by a common source, a paper mill , can be found here: * https: forbetterscience.com 2020 01 24 the-full-service-paper-mill-and-its-chinese-customers (January 2020) * https: scienceintegritydigest.com 2020 02 21 the-tadpole-paper-mill (February 2020) The same paper mill has been described in these papers: * Jana Christopher - Systematic fabrication of scientific images revealed - FEBS Letters (2018) - https: febs.onlinelibrary.wiley.com doi full 10.1002 1873-3468.13201 * Jennifer A Byrne and Jana Christopher - Digital magic, or the dark arts of the 21st centuryu2014how can journals and peer reviewers detect manuscripts and publications from paper mills? - FEBS Letters (2020) - https: febs.onlinelibrary.wiley.com doi full 10.1002 1873-3468.13747 , 
 #1 I have concerns about the provenance of other data. *[left]* Fig 2C. *[right]* Fig 8 from  Numb Promotes Cell Proliferation and Correlates with Poor Prognosis in Hepatocellular Carcinoma ([Wu et al 2014] ) - rotated through 90 deg for ease of comparison.  ------------------------------------ Figs 3A,C and 5A,E:  Fig 4A,B from  SOX2 Enhances the Migration and Invasion of Ovarian Cancer Cells via Src Kinase ([Wang et al 2014] ):  , 
  Other versions of these panels appear in other products of the Tadpole papermill. Fig 2 of  MicroRNA-940 Targets INPP4A or GSK3u03b2 and Activates the Wnt u03b2-Catenin Pathway to Regulate the Malignant Behavior of Bladder Cancer Cells ([Wang et al 2018] ):  Fig 2G of  miR-203 Suppresses Bladder Cancer Cell Growth and Targets Twist1 ([Shen et al 2018] ).  ,