Lnc-SNHG1 may promote the progression of non-small cell lung cancer by acting as a sponge of miR-497
Authors: Zhanxia Li
Dongyi Zhu
Yang Han
Xiaohui Zhou
Tao Ren
Qingchun Lu
Journal: Biochemical and Biophysical Research Communications (2018)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: IGF1-R; Long noncoding RNA; Non-small cell lung cancer; SNHG1; miR-497.
Affiliations: Department of Intensive Care Unit, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China., Department of Respiratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China., Department of Pathology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China., Department of Respiratory Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China. Electronic address: ., Department of Respiratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China; Department of Respiratory Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China. Electronic address: .

Abstract:  Lnc-SNHG1 (small nucleolar RNA host gene 1) is considered an important regulating factor in several types of cancers. However, the biological functions and underlying molecular mechanisms in which lnc-SNHG1 is involved in non-small cell lung cancer (NSCLC) still need to be explored. In this study, we investigated the detailed effects and possible molecular mechanisms. The transcript level of lnc-SNHG1 was higher in lung adenocarcinoma specimens and NSCLC cell lines than in noncancer tissue and cells. The level of expression was positively correlated with invasiveness and was negatively correlated with the level of miR-497 in vivo and in vitro. In exploring the regulatory mechanism, we found that lnc-SNHG1 might modulate tumor growth by sponging miR-497. The inhibitory effect of si-lnc-SNHG1 on NSCLC cell proliferation, migration and invasion could be rescued by miR-497 inhibition, while the overexpression of miR-497 could reverse the effect of lnc-SNHG1 overexpression. Furthermore, our study demonstrated that the lnc-SNHG1 regulated the expression of the insulin-like growth factor 1 receptor (IGF1-R) by acting as a sponge of miR-497 in NSCLC. lnc-SNHG1 could be a novel biomarker as well as a curative target. 

Retracted: No

PubPeer comments:
 Figure 4D. Cyclin E and Lamin B panels look like they come from the same blot. Cyclin A and cyclin D1 panels do not look they come from the same blots as the Cyclin E and Lamin B panels, and do not look like they came from the same blot as each other. https: imgur.com PK1113p , 
 I am not entirely sure I would call this a top tier scientific problem and I presume it has little to do with Leucula Festiva being unable to reproduce the data or having issues with the conclusions. I think this is fine, because some of these proteins are close in size, so the authors could strip the blot and re-probe which has all sorts of problems and is a much bigger source of unintentional error, when compared to running two gels from the same protein lysate and blotting for proteins in adjacent gels and just being conscious of making sure loading is even. Or they could strip the blot two times, to re-probe three times (to get the three 50-60kDa-ish proteins from the same gel), which I would trust far less. Regardless, since the paper is 12 years old anyway, is it fair to raise a micro-concern like this? ,