MicroRNA-215 targets NOB1 and inhibits growth and invasion of epithelial ovarian cancer
Authors: Manhua Cui
Tianmin Xu
Yang Jin
Yang Lin
Shunqing Zhou
Journal: American journal of translational research (2017)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: MicroRNAs; NOB1; epithelial ovarian cancer; miR-215.
Affiliations: Department of Obstetrics and Gynecology, The Second Hospital, Jilin University #218 Ziqiagn Street, Nanguan District, Changchun 130041, China., School of Pharmaceutical Sciences, Jilin University Changchun 130021, China.

Abstract:  MicroRNA-215 (miR-215) has been showed to play crucial roles in tumorigenesis and tumor progression in many types of cancer. However, its biological function and underlying mechanism in epithelial ovarian cancer (EOC) remains greatly unknown. The aims of this study were to investigate biological role and underlying mechanism of miR-215 in EOC. Here, we found that miR-215 expression was significantly decreased in EOC tissues or cell lines compared with adjacent normal tissues or normal ovarian cell line. Decreased miR-215 expression was significantly associated with International Federation of Gynaecology and Obstetrics (FIGO) stage and lymph node metastasis. Function analysis revealed that overexpression of miR-215 using miR-215 mimic significantly inhibit EOC cell proliferation, colony formation, migration and invasion in vitro. as well as suppress tumor growth in vivo. Moreover, we identified ribosome assembly factor NIN/RPN12 binding protein (NOB1) as a direct targets for miR-215 binding, resulting in suppression it expression, which in turn activated the MAPK signaling pathway. In clinical EOC specimens, NOB1 expression was upregulated, and inversely correlated with miR-215 expression (r = -0.675, P&lt;0.001). Overexpression of NOB1 effectively rescued inhibition effect on EOC cells by induced miR-215 overexpression. Taken together, our findings suggested that miR-215 suppressed EOC growth and invasion by targeting NOB1. 

Retracted: Yes

PubPeer comments:
 *[right]* Fig 4.  miR-215 activate MAPK signal pathway. The p38MAPK, phosphorylated-p38MAPK (p-p38MAPK), ERK, p-ERK, JNK and p-JNK protein expression were measured in SKOV3 cells transfected with miR-215 and miR-NC or infected with Lv sh-NOB1 or Lv shScramble by Western blot . *[left]* Fig 5 from [Liu et al (2015)] . I have concerns.  UPDATE: A few more bands coloured now, to show affiliations to Fig 4D of [Li et al (2016)] .  IGF1R, AKT, p-AKT, ERK, and p-ERK protein in SKOV3 cells transfected with miR-494 mimic were determined by western blot .  , 
 Fig 5D.  Cell ... colony formation ... were determined in [cotransfected SKOV3] cells.  Rotated through 180 deg, the left-hand plate also appears as Fig 2C from [Li et al (2017)] .  Cell colony formation of A549 cells transfected with miR-592 mimic or miR-Ctrl was analyzed .  Fig 2C from [Cao et al (2017)] .  colony formation ... determined in 786-O cells after transfection with miR-148a mimic or miR-NC .  , 
 *[right]* Fig 6B.  Graphs of the tumor tissues . *[left]* Fig 7B from [Han et al (2016)] .  Tumor size and weight.  These appear to be the same 8 tumors, in two different arrangements. , 
 Retracted, [August 15 2021] .  Regarding this article, we recently discovered that the results reported in that publication cannot be reproduced. Therefore, we wish to withdraw this paper. We sincerely apologize for any inconvenience caused by the retraction of this paper. ,