Retracted: Effects of MicroRNA‚Ä?9b on the Proliferation, Apoptosis, and Migration of Wilms‚Ä?Tumor Cells Via the PTEN/PI3K/AKT Signaling Pathway
Authors: Ge-Liang Liu
Bo Liu
Han-Jie Yang
Tian Liu
Journal: Journal of Cellular Biochemistry (2017)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: CELL APOPTOSIS; CELL MIGRATION; CELL PROLIFERATION; MICRORNA-19B; PTEN/P13K/AKT SIGNALING PATHWAY
Affiliations: Department of Urinary Surgerythe Affiliated Pingxiang HospitalSouthern Medical UniversityPingxiang337000P.R. China, Department of General SurgeryThe Second Xiangya Hospital of Central South UniversityChangsha410011P.R. China

Abstract:  Wilms' tumor (WT) is a most common renal cancer that occurs among children, and microRNA-19b (miR-19b) usually participates in various human cancers. Importantly, the PTEN/PI3K/Akt signaling pathway plays a key role in cell apoptosis, growth and proliferation. Thus, our present study aims to investigate the effect of miR-19b on the PTEN/PI3K/Akt signaling pathway during WT cell proliferation, migration, and apoptosis. WT tissues and adjacent normal tissues from WT patients were collected. qRT-PCR was applied to detect miR-19b expression in both the WT tissues and the adjacent normal tissues, immunohistochemistry was applied to detect the protein expressions of PTEN, P13K, and p-Akt, SK-NEP-1 cells were divided into the blank, negative control (NC), miR-19b mimics and miR-19b inhibitors groups. MTT assay, propidium iodide (PI) staining, Annexin-V/PI double-staining, Transwell assay and Western blotting were performed to examine cell proliferation, cycle, apoptosis, migration, and invasion, and the protein expressions of PTEN, P13K, Akt, and p-Akt. Increased miR-19b expression, positive expression rates of P13K and Akt, decreased PTEN expression rate, a negative correlation between PTEN expression and tumor lymph node metastasis, and a positive correlation between the expression of P13K and Akt and the clinical stages were observed in the WT tissues. The miR-19b inhibitors group exhibited decreased cell proliferation, cell cycle progression, migration and invasion, and protein expressions of PI3K and p-Akt but increased PTEN protein expression compared with the blank and NC groups. Thus, inhibition of miR-19b suppresses the progression of WT by modulating the PTEN/PI3K/AKT signaling pathway. J. Cell. Biochem. 118: 3424-3434, 2017. ¬© 2017 Wiley Periodicals, Inc. 

Retracted: Yes

PubPeer comments:
 Fig. 5A.  Cell cycle and the apoptosis rate of transfected cells as detected by the PI single-staining method and the V PI Annexin double-staining method. Note: A, The results of flow cytometry for cell cycle analysis after transfection . All four examples seem to show the same raw data of cell counts, with different vertical scales and fitted with different model distributions.  , 
 Fig 6.  The migration and invasion ability of the SK-NEP-1 cells after transfection in each group as detected by the Transwell assay .  Fig 7A from [Wang et al (2019)] .  LncRNA HOST2 silencing represses U251and U87 cell invasion as detected by Transwell assay. (A) The cells passing through the bottom chamber were stained with crystal violet .  Different cell types, different treatments, different authors, same panel. , 
 Fig 6 again:  Fig 8A of [Li et al (2017)] .  Cell invasion of normal cells, and A431 and SCL-1 cells in the blank, NC, miR-374 mimics, miR374 inhibitors, siRNAu2013Gadd45a, and miR-374 inhibitors + siRNAu2013Gadd45a groups, evaluated by the Transwell assay (A) A431 cell invasion images :  Different cell types, different treatments, different authors, same panel. , 
 Retracted [30 June 2021] . ---------------------------- The above article, published online on 21 March 2017 in Wiley Online Library has been retracted by agreement between the the journal s Editor in Chief, Prof. Dr. Christian Behl, and Wiley Periodicals LLC. The retraction has been agreed following an investigation based on allegations raised by a third party. A detailed investigation revealed that several image elements of the experimental data were published elsewhere in a different scientific context. Thus, the editors consider the conclusions of this article to be invalid. ,