Understanding the anti-kindling role and its mechanism of Resveratrol in Pentylenetetrazole induced-kindling in a rat model
Authors: Amitava Chakrabarti
Lekha Saha
Journal: Pharmacology Biochemistry and Behavior (2014)
Discipline: NEUROSCIENCE & BEHAVIOR
Keywords: Antioxidant; Epileptogenesis; Kindling; Pentylenetetrazole; Rat
Affiliations: Department of Pharmacology, Post Graduate Institute of Medical Education & Research (PGIMER), Chandigarh, India. Electronic address: ., Department of Pharmacology, Post Graduate Institute of Medical Education & Research (PGIMER), Chandigarh, India.

Abstract:  Background: Resveratrol is a polyphone chemical found in a number of plant species, including peanuts and grapes, but with significant amounts in red wine. In normal plant physiology, Resveratrol is produced as a defensive response to injury or parasitic attacks. Resveratrol has diverse biological properties and actions with potential clinical applications, including anti-inflammatory, antioxidant, anti proliferative, and neuroprotective effects. Aim: The aim of the present study was to explore the effect and mechanism of Resveratrol in Pentylenetetrazole (PTZ) induced kindling in rats. Materials and methods: In a PTZ kindled Wistar rat model, different doses of Resveratrol (25mg/kg, 50mg/kg and 75 mg/kg) were administered orally 30 min before the PTZ injection. The PTZ injection was given on alternate day till the animal became fully kindled or till 10 weeks. The following parameters were compared between control and various experimental groups: the course of kindling, stages of seizures, histopathological scoring of hippocampus, antioxidant parameters, DNA fragmentation and caspase-3 expression in the hippocampus, and neuron-specific enolase in the blood. One way ANOVA followed by Bonferroni post hoc analysis and Fischer's Exact test were used for statistical analyses. The results: In the present study, Resveratrol showed dose-dependent anti-seizure effect. Resveratrol (75 mg/kg) significantly increased the latency to myoclonic jerks, clinic seizures as well as generalized tonic-clinic seizures, improved the seizure score and decreased the number of myoclonic jerks. PTZ induced kindling caused a significant neuronal injury, oxidative stress and apoptosis which were reversed by pretreatment with Resveratrol in a dose-dependent manner. Conclusion: Our study suggests that Resveratrol has a potential antiepileptogenic effect on PTZ-induced kindling in rats. The possible underlying mechanisms of Resveratrol as an antiepileptic agent may be due to its antioxidative property and neuroprotective effect. 

Retracted: No

PubPeer comments:
 The Tables 1 in three of the four papers listed above also show remarkable similar values - some marked here, but several others to be found.  , 
 Figure 2 of this paper appears to share panels with Figure 2 in the Journal of Epilepsy Research 2014 paper mentioned above. Note the panels on the right (marked in aqua boxes, and mislabeled C U K in the PBB paper), which appear to represent different treatments.  , 
 Figure 3 in this paper looks remarkably similar to figures in three other papers by shared researchers. Thus, in total, four papers appear to show the same gel. The four papers are: * Lekha Saha et al. - Anti-kindling Effect of Bezafibrate, a Peroxisome Proliferator-activated Receptors Alpha Agonist, in Pentylenetetrazole Induced Kindling Seizure Model - Journal of Epilepsy Research 2014; 4(2): 45-54.t- DOI: 10.14581 jer.14011 - https: pubpeer.com publications 5DA39E0F9B3FECB5FB244FBF4718A4 * Lekha Saha et al. - Understanding the anti-kindling role and its mechanism of Resveratrol in Pentylenetetrazole induced-kindling in a rat model - Pharmacology, Biochemistry and Behavior 120 (2014) 57u201364 - DOI: 10.1016 j.pbb.2014.01.010 - https: pubpeer.com publications A5EAA270F953F8F1553C8E3B542D53 * Lekha Saha et al. - Gastroprotective effect of bezafibrate, a peroxisome proliferator activated receptor a agonist and its mechanism in a rat model of aspirin-induced gastric ulcer - Advances in Digestive Medicine (2016) -DOI: 10.1016 j.aidm.2016.04.001 - https: pubpeer.com publications 37BC35FD27C1AB7130178F6716C2B7 * Lekha Saha et al., - Antiapoptotic and neuroprotective role of Curcumin in Pentylenetetrazole (PTZ) induced kindling model in rat - Indian J Exp Biol . 2016 Feb;54(2):133-41 - PMID: 26934781 - https: pubpeer.com publications 18EC51EB813A08FCA5D010C358C376 Despite the different experiments, the gels look very similar to each other. In addition, in each of these, lanes 1 2 3 4 9 look remarkably similar. Yellow boxes showing the possible lane similarities are only shown in one of the four versions.   ,