Retracted: Use of Decellularized Scaffolds Combined with Hyaluronic Acid and Basic Fibroblast Growth Factor for Skin Tissue Engineering
Authors: Xing Wei
Peng Zhang
Bin Xu
Lina Fan
Zhengzheng Wu
Yongliang Lin
Journal: Tissue Engineering Part A (2015)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Skin damage; skin graft; xenogeneic decellularized scaffold; pig peritoneum; hyaluronic acid
Affiliations: 1 Institute of Biomedicine, College of Life Science and Technology, National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, China ., Multidisciplinary Research Center, College of Science, Shantou University, Shantou, China., Grandhope Biotech Co. Ltd, Guangzhou International Business Incubator, Guangzhou Science Park, Guangzhou, China., Institute of Biomedicine, College of Life Science and Technology, National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, China.

Abstract:  Skin damage is one of the common clinical skin diseases, and the main cure is the use of skin graft, especially for large area of skin injury or deep-skin damage. However, skin graft demand is far greater than that currently available. In this study, xenogeneic decellularized scaffold was prepared with pig peritoneum by a series of biochemical treatments to retain normal three-dimensional tissue scaffold and remove cells and antigenic components from the tissue. Scaffold was combined with hyaluronic acid (HA) plus two different concentrations of basic fibroblast growth factor (bFGF) and tested for its use for the repair of skin wounds. HA enhanced bFGF to adsorb to the decellularized scaffolds and slowed the release of bFGF from the scaffolds in vitro. A total of 20 rabbits were sacrificed on day 3, 6, 11, or 14 postsurgery. The wound healing rate and the thickness of dermis layer of each wound were determined for analyzing the wound repair. Statistical analysis was performed by the two-tailed Student's t-test. Wounds covered with scaffolds containing 1 Œºg/mL bFGF had higher wound healing rates of 47.24%, 74.69%, and 87.54%, respectively, for days 6, 11, and 14 postsurgery than scaffolds alone with wound healing rates of 28.17%, 50.31%, and 61.36% and vaseline oil gauze with wound healing rates of 24.84%, 42.75%, and 57.62%. Wounds covered with scaffolds containing 1 Œºg/mL bFGF showed more dermis regeneration than the other wounds and had dermis layer of 210.60, 374.40, and 774.20 Œºm, respectively, for days 6, 11, and 14 postsurgery compared with scaffolds alone with dermis layer of 116.60, 200.00, and 455.40 Œºm and vaseline oil gauze with dermis layer of 82.60, 186.20, and 384.40 Œºm. There was no significant difference in wound healing rates and thickness of dermis layer between wounds covered with scaffolds containing 1 and 3 Œºg/mL bFGF on days 3, 6, 11, and 14 postsurgery. The decellularized scaffolds combined with HA and bFGF can be further tested for skin tissue engineering. 

Retracted: Yes

PubPeer comments:
 Some apparent image duplications in figures 3, 5, and 6. Sometimes the images were rotated.    There could be more, I had trouble due to the small image size. , 
 Duplicate comment, please ignore ,