Circ_0105346 Knockdown Inhibits Osteosarcoma Development via Regulating miR-1182/WNT7B Axis
Authors: Jinbao Liu
Liang Yue
Hao Lv
Xiaoyang Li
Journal: Cancer Management and Research (2021)
Discipline: CLINICAL MEDICINE
Keywords: WNT7B; apoptosis; circ_0105346; glycolysis; metastasis
Affiliations: Department of Orthopaedics, The First Clinical Medical School, Shandong University of Traditional Chinese Medicine, Jinan 250011, People's Republic of China., Department of Pediatric Orthopaedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, People's Republic of China.

Abstract:  Background: Osteosarcoma (OS) is a common bone malignancy in children and adolescents. Circular RNAs (circRNAs) have been reported to affect OS progression. This paper mainly delineated the role of circRNA circ_0105346 in OS development and the potential mechanism. Methods: Quantitative reverse transcription PCR (qRT-PCR) and Western blot assays were applied to detect the expression of circ_0105346, microRNA (miR)-1182 and wingless-type MMTV integration site family 7B (WNT7B). 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay was conducted to evaluate cell viability, and flow cytometry was performed to monitor cell apoptosis and cycle. In addition, cell migration and invasion were determined via transwell assay. Wound healing assay was also employed to evaluate the migrated capacity of OS cells. Western blot assay was also employed to examine the levels of protein markers. Additionally, the interaction between miR-1182 and circ_0105346 or WNT7B was confirmed by the dual-luciferase reporter, RNA immunoprecipitation (RIP) and pull-down assays. Mouse xenograft model was constructed to clarify the effect of circ_0105346 on tumor growth in vivo. Results: Circ_0105346 and WNT7B were upregulated, while miR-1182 was downregulated in OS tissues and cells. Circ_0105346 knockdown suppressed OS cell proliferation, cell cycle, migration, invasion and glycolysis, as well as accelerated apoptosis, which was attenuated by miR-1182 inhibition. Interestingly, circ_0105346 targeted miR-1182, and miR-1182 interacted with WNT7B. Circ_0105346 could upregulate WNT7B by downregulating miR-1182 expression. Furthermore, circ_0105346 knockdown blocked tumor growth in vivo. Conclusion: Circ_0105346 knockdown repressed OS progression by regulating miR-1182/WNT7B axis, at least in part. 

Retracted: Yes

PubPeer comments:
 Retracted [30 June 2021] . ---------------------------------- The Editor and Publisher of Cancer Management and Research wish to retract the published paper. The authors initially requested the retraction claiming some results described in the initial study could not be repeated and there were concerns with the specificity of the u03b2-actin primer used in the qRT-PCR assay. Despite several requests from the editorial office, the authors could not provide satisfactory raw data for their qRT-PCR assay. They were also unable to provide satisfactory raw data for the western blots described in the study. It was determined the findings of the study were invalid and the decision was made to retract the paper. Our decision-making was informed by our policy on publishing ethics and integrity and the COPE guidelines on retraction. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as u201cRetractedu201d. -------------------------------------------- Here are figures 4M,N as examples of the Western Blots that the authors were unable to validate.  ,