Ubiquitin‚Äëproteasomes are the dominant mediators of the regulatory effect of microRNA‚Ä? on cardiac remodeling after myocardial infarction
Authors: Yue Xu
Yufan Zhang
Yuanyang Li
Liping Wei
Xin Qi
Xuseng Sun
Journal: International Journal of Molecular Medicine (2019)
Discipline: CLINICAL MEDICINE
Keywords: Ischemic hearts; myocardial remodeling; miRNA-1; ubiquitin-proteasome system; myocardial infarction
Affiliations: Department of Cardiology, Tianjin Union Medical Center, Nankai University Affiliated Hospital, Tianjin 300121, P.R. China., School of Graduate Studies, Tianjin Medical University, Tianjin 300070, P.R. China., School of Graduate Studies, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, P.R. China.

Abstract:  Patients with ischemic hearts who have refused coronary vascular reconstruction may exhibit dynamic myocardial remodeling and cardiac dysfunction. In the present study, the role of miRNA‚Ä? and its association with the ubiquitin‚Äëproteasome system (UPS) in regulating myocardial remodeling was investigated. A myocardial infarction (MI) model was constructed and the hearts were treated with miRNA‚Ä? antagomir, miRNA‚Ä? lentiviral vectors and the UPS proteasome blocker bortezomib. The expression levels of miRNA‚Ä? were evaluated using reverse transcription PCR and the abundance of the ubiquitin‚Äëproteasome protein and caspase‚Ä? were evaluated via western blot analysis. Furthermore, the collagen volume fraction was calculated using Masson's trichrome staining, and the apoptosis index was detected via terminal deoxynucleotidyl transferase dUTP‚Äëbiotin nick end labeling staining. Transforming growth factor (TGF)‚ÄëŒ?expression was assessed via immunohistochemical staining. Echocardiographic characteristics and myocardial infarct size were analyzed. miRNA‚Ä? expression levels were found to be increased following MI. miRNA‚Ä? antagomir administration clearly inhibited miRNA‚Ä? expression, whereas the miRNA‚Ä? lentiviral vector exerted the opposite effect. The levels of 19s proteasome, 20S proteasome and ubiquitin ligase E3 were decreased in the miRNA‚Ä? antagomir group, but were significantly increased in the miRNA‚Ä? lentiviral group; however, only 20S proteasome expression was decreased in the bortezomib group. Collagen hyperplasia and TGF‚ÄëŒ?expression were decreased in both the miRNA‚Ä? antagomir and bortezomib groups, although the effects of the miRNA‚Ä? antagomir were more noticeable. The miRNA‚Ä? antagomir and the UPS proteasome blocker both alleviated the ultrastructural impairments, demonstrated by a decreased left ventricular (LV) end‚Äëdiastolic diameter and LV mass, but the miRNA‚Ä? antagomir was also able to increase LV ejection fraction and LV fractional shortening. miRNA‚Ä? regulated UPS‚Äëassociated mRNA expression and affected the majority of the UPS components in the myocardium, thereby leading to increased myocardial cell apoptosis, myocardial fibrosis and remodeling. The miRNA‚Ä? antagomir exerted a more prominent cardioprotective effect compared with the UPS proteasome blocker bortezomib. 

Retracted: No

PubPeer comments:
  Figure 5 - Myocardial infarct size in each group. Representative illustrations of myocardial infarct in different groups. The white area represents myocardial infarct tissue. (A) Myocardial infarct size was calculated and evaluated . MI and MI+control are not identical but surprisingly similar.  , 
 #1 These two groups are the control group without drugs. We think they represent the same infarct degree, so we choose the photo with similar infarct degree. Thank you! , 
 This experiment was conducted by myself. Both groups were not given the drug.The infarcts were almost the same size for several trials, but in fact only one group may be sufficient for some trials. I think they can represent each other. Thank youuff01 ,