MiR-143 regulates proliferation and apoptosis of myelocytic leukemia cell HL-60 via modulating ERK1
Authors: W-G Zhang
C-C Wan
B Song
L-J Zhang
Y Chen
Y-J Tang
Journal: European review for medical and pharmacological sciences (2018)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: ERK1; miR-143; leukemia; proliferation; apoptosis
Affiliations: Department of Hematology, Affiliated Taihe Hospital of Xi'an Jiaotong University Health Science Center, Shiyan, Hubei, China. .

Abstract:  Objective: Extracellular signal-regulated kinase (ERK)/mitogen activated protein kinase (MAPK) signaling pathway is widely involved in cell proliferation and invasion regulation. Enhanced expression or function of ERK1 is important for leukemia. Abnormal down-regulation of microRNA (miR)-143 is correlated with leukemia pathogenesis, indicating possible tumor-suppressing role. Bioinformatics analysis showed the existence of complementary binding sites between miR-143 and ERK1. This study aims to investigate whether the miR-143 plays a role in mediating ERK1 expression and proliferation and apoptosis of leukemia cells. Patients and methods: Dual luciferase reporter gene assay confirmed targeted regulation between miR-143 and ERK1. Quantitative RT-PCR (qRT-PCR) was used to measure and compare the peripheral miR-143 and ERK1 expression between healthy and acute promyelocytic leukemia (APL) patients to analyze the effect of miR-143 and MEK1 on survival and prognosis. Cultured HL-60 cells were treated with miR-143 mimic or small interfering RNA (siRNA)-ERK1, followed by qRT-PCR to measure miR-143 expression. Western blot quantified expression of ERK1 and p-ERK1, flow cytometry measured apoptosis, and EdU staining measured proliferation. Results: MiR-143 targeted and modulated ERK1. APL patients presented lower miR-143 and higher ERK1 in peripheral blood. Those with miR-143 down-regulation displayed worse prognosis than those with high miR-143 expression (œá2 = 5.198, p = 0.039). Patients with ERK1 mRNA low-expression presented better prognosis than those a having higher expression (Log-rank test, œá2 = 5.873, p = 0.028). Transfection of miR-143 mimic or siRNA-ERK1 remarkably suppressed ERK1 and p-ERK1 expression in HL-60 cells, inhibited cell proliferation and induced cell apoptosis. Conclusions: MiR-143 down-regulation and ERK1 up-regulation are correlated with APL pathogenesis. Their expression level affected patient's prognosis. MiR-143 targeted and inhibited ERK1 expression, weakened proliferation potency of HL-60 cells, and induced apoptosis. 

Retracted: Yes

PubPeer comments:
 A Gallery of Unfortunate FACS Plots. Please excuse the length. This is a random assortment from a certain oeuvre. Any other of ~40 papers from the same journal(s) could have been used instead. I have only marked a few of the recurring constellations of points. Colored plots darkened and contrast-enhanced for visibility. Fig 4C [Liu et al (2017)] PMID 28770966 Fig 2D, 3D [Wu et al (2017)] PMID 28617558 Fig 2D [Liang et al (2017)] PMID 29164556  Fig 3E [Li et al (2018)] 10.26355 eurrev_201809_15933 Fig 4D [Dong et al (2018)] 10.26355 eurrev_201805_15059 Fig 4D [Song et al (2018)] 10.26355 eurrev_201806_15153  Fig 2B, 4B, 5C [Wang et al (2018)] 10.26355 eurrev_201801_14201 Fig 3B, 4E [Su Song (2018)] 10.26355 eurrev_201810_16155 Fig 2D [Han et al (2018)] 10.26355 eurrev_201811_16390  Fig 4C [Wang Yu (2019)] 10.26355 eurrev_201903_17397 Fig 5D [Zhou et al (2019)] 10.26355 eurrev_201911_19436 Fig 4E [Liu Zhang (2019)] 10.1089 cbr.2018.2749 Fig 4D [Piao et al (2019)] 10.1089 cbr.2018.2625  , 
 It may also be worth documenting the curious stylized renditions of Western Blots favoured by this production studio (and by the editors and reviewers who accept this oeuvre of papers).  , 
 Withdrawn. https: www.europeanreview.org article 22994 *Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, u201cMiR-143 regulates proliferation and apoptosis of myelocytic leukemia cell HL-60 via modulating ERK1, by B. Song, Y.-J. Tang, W.-G. Zhang, C.-C. Wan, Y. Chen, L.-J. Zhang, published in Eur Rev Med Pharmacol Sci 2018; 22 (11): 3333-3341u2013DOI: 10.26355 eurrev_201806_15153u2013PMID: 29917183u201d has been withdrawn.* *The Publisher apologizes for any inconvenience this may cause.* ,