Molecular identification of head lice collected in Franceville (Gabon) and their associated bacteria
Authors: Nadia Amanzougaghene
Celia Scherelle Boumbanda-Koyo
Jean Bernard Lekana-Douki
Rom√©o Karl Imboumi-Limoukou
Oleg Mediannikov
Florence Fenollar
Didier Raoult
Sandrine Lydie Oyegue-Liabagui
Journal: Parasites & Vectors (2020)
Discipline: MICROBIOLOGY
Keywords: Acinetobacter spp.; Borrelia spp.; Gabon; Head lice; Pediculus humanus.
Affiliations: Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France., IHU-M√©diterran√©e Infection, Marseille, France., Unit√© d'Evolution, Epid√©miologie et R√©sistances Parasitaires (UNEEREP), Centre International de Recherches M√©dicales de Franceville (CIRMF), B.P. 769, Franceville, Gabon., Ecole Doctorale R√©gionale en Infectiologie Tropicale d'Afrique Centrale, B.P. 876, Franceville, Gabon., IHU-M√©diterran√©e Infection, Marseille, France. ., Aix Marseille Univ, IRD, AP-HM, MEPHI, Marseille, France. ., Aix Marseille Univ, IRD, AP-HM, MEPHI, Marseille, France., D√©partement de Parasitologie-Mycologie et M√©decine Tropicale, Facult√© de M√©decine, Universit√© des Sciences de la Sant√© (USS), B.P. 4009, Libreville, Gabon.

Abstract:  Background: Pediculus humanus, which includes two ecotypes (body and head lice), is an obligate bloodsucking parasite that co-evolved with their human hosts over thousands of years, thus providing a valuable source of information to reconstruct the human migration. Pediculosis due to head lice occurred each year throughout the world and several pathogenic bacteria, which are usually associated with body lice, are increasingly detected in them. In Gabon, where this pediculosis is still widespread, there is a lack of data on genetic diversity of head lice and their associated bacteria. Methods: This study aimed to investigate the phylogeny of head lice collected in Gabon and their associated bacteria, using molecular tools. Between 26 March and 11 April 2018, 691 head lice were collected from 86 women in Franceville. We studied the genetic diversity of these lice based on the cytochrome b gene, then we screened them for DNA of Bartonella quintana, Borrelia spp., Acinetobacter spp., Yersinia pestis, Rickettsia spp., R. prowazekii, Anaplasma spp. and C. burnetii, using real time or standard PCR and sequencing. Results: Overall 74.6% of studied lice belonged to Clade A, 25.3% to Clade C and 0.1% to Clade E. The phylogenetic analysis of 344 head lice yielded 45 variable positions defining 13 different haplotypes from which 8 were novel. Bacterial screening revealed the presence of Borrelia spp. DNA in 3 (0.4%) of 691 head lice belonging to Clade A and infesting one individual. This Borrelia is close to B. theileri (GenBank: MN621894). Acinetobacter spp. DNA has been detected in 39 (25%) of the 156 screened lice; of these 13 (8.3%) corresponded to A. baumannii. Acinetobacter nosocomialis (n = 2) and A. pittii (n = 1) were also recorded. Conclusions: To of our knowledge, this study is the first to investigate the genetic diversity of head lice from Gabon. It appears that Clade C is the second most important clade in Gabon, after Clade A which is known to have a global distribution. The detection of Borrelia spp. DNA in these lice highlight the potential circulation of these bacteria in Gabon. 

Retracted: No

PubPeer comments:
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 Flow cytometry plots in Figure 11 show some usual areas of similarity. One example below. ***  *** Closeup comparison  *** Full figure  ,