MicroRNA-144 acts as a tumor suppressor by targeting Rho-associated coiled-coil containing protein kinase 1 in osteosarcoma cells
Authors: Hui Li
Meisongzhu Yang
Jianjun Huang
Guohong Liu
Ying Shi
Journal: Molecular Medicine Reports (2015)
Discipline: CLINICAL MEDICINE
Keywords: MicroRNAs; miR-144; osteosarcoma; cell proliferation; migration
Affiliations: The Second Department of Orthopedics, The First Affiliated Hospital of Jishou University, Jishou, Hunan 416000, P.R. China., Teaching and Research Department of Pathology and Pathophysiology, Medical School of Jishou University, Jishou, Hunan 416000, P.R. China., Department of Immunology Microbiology, Medical School of Jishou University, Jishou, Hunan 416000, P.R. China.

Abstract:  MicroRNAs (miRs) have been demonstrated to be associated with multiple processes in the development and progression of human malignancies. Previous studies have observed aberrant downregulation of miR‚Ä?44 in several types of cancer, including osteosarcoma. However, the function of miR‚Ä?44 and the underlying mechanism in osteosarcoma remain to be elucidated. The present study indicated that miR‚Ä?44 was markedly downregulated in osteosarcoma tissues and cell lines compared with that in the normal controls. Restoration of miR‚Ä?44 significantly inhibited cell proliferation, migration and invasion of MG‚Ä?3 osteosarcoma cells. In addition, Rho‚Äëassociated coiled‚Äëcoil containing protein kinase 1 (ROCK1) was identified as a novel target of miR‚Ä?44 in MG‚Ä?3 osteosarcoma cells. Furthermore, knockdown of ROCK1 suppressed the proliferation, migration and invasion of MG‚Ä?3 osteosarcoma cells to a similar extent to the effects of miR‚Ä?44 overexpression. In addition, the mRNA expression of ROCK1 was increased in osteosarcoma tissues and was negatively correlated with the expression of miR‚Ä?44. In conclusion, the results of the present study suggested that miR‚Ä?44 acts as a tumor suppressor by targeting ROCK1 in osteosarcoma. 

Retracted: Yes

PubPeer comments:
 Figs 2C, D and 4 B, D.  (C) Inhibitory effect of miR-144 on the **migration** of MG-63 cells.... (D) Inhibitory effect of miR-144 on the **invasion** of MG-63 cells ;  (B) Knockdown of ROCK1 inhibited the **migration** capacity of MG-63 cells .... (D) Knockdown of ROCK1 inhibited the **invasion** capacity of MG-63 cells .  Images are all Transwell invasion assays. I set up a spreadsheet to include the various papers using this versatile suite of Transwell images to represent all possible combinations of cell-lines and treatments. This is the 42nd manifestation. For comparison: Fig 3 C D from [Zhang et al (2016)] .  Transwell assay were performed to examine the migratory and invasive capacities of Saos-2 and U2OS cells transfected with miR-205 mimic or miR-negative control (NC) mimic , and Fig 7C D,  Transwell assay were performed to examine cell migration and invasion of MG63 or U2OS cells transfected with miR-205 mimic, or co-transfected with miR-205 mimic and RUNX2 plasmid .  Figs 4D, 6D from [Xia et al (2016)] .  Also *[above]* Fig 4C from [Lai, Chen Li (2015)] .  a Transwell assay were performed to examine the cell proliferation of AGS cells transfected with miR-133a mimics, miR-133a inhibitor, or co-transfected with miR-133a mimics and FSCN1 plasmid. Control: AGS cells without any transfection. *[below]* Figs 3(B), 6(B) from [Shi et al (2015)] .  Transwell assay was performed to determine the invasive capacity of the Saos-2 and U-2 OS cells transfected with NC scramble miRNA or miR-204 mimics, respectively. Control, Saos-2 and U-2 OS cells without any transfection ...  Transwell assay was performed to determine the invasive capacity of the Saos-2 and U-2 OS cells transfected with Sirt1 siRNA or co-transfected with miR-204 mimics and the Sirt1 plasmid, respectively. Control, Saos-2 and U-2 OS cells without any transfection .  , 
 Retracted [5 August 2021] . -------------------------------- Following the publication of this paper, it was drawn to the Editorsu2019 attention by a concerned reader that certain of the Transwell cell migration data shown in Figs. 2C and D, and 4B and D, were strikingly similar to data appearing in different form in other articles by different authors. Owing to the fact that the contentious data in the above article had already been published elsewhere, or were already under consideration for publication, prior to its submission to *Molecular Medicine Reports*, the Editor has decided that this paper should be retracted from the Journal. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive any reply. The Editor apologizes to the readership for any inconvenience caused. ,