Long noncoding RNA OIP5-AS1 promotes the progression of oral squamous cell carcinoma via regulating miR-338-3p/NRP1 axis
Authors: Zhihong Li
Jun Ning
Lei Wang
Qianyi Fei
Yue Ge
Minghe Li
Cong Zhao
Journal: Biomedicine & Pharmacotherapy (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: NRP1; OIP5-AS1; Oral squamous cell carcinoma; miR-338-3p.
Affiliations: Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jilin University, Changchun, 130021, PR China., Department of Gynaecology II, The First Hospital of Jilin University, Changchun, 130021, PR China., Department of Thoracic Surgery, The First Hospital of Jilin University, Changchun, 130021, PR China., Department of Orthodontics, Hospital of Stomatology, Jilin University, Changchun, 130021, PR China., Departments of Periodontology, Hospital of Stomatology, Jilin University, Changchun, PR China. Electronic address: .

Abstract:  Opa-interacting protein 5 antisense RNA 1 (OIP5-AS1), a novel identified long noncoding RNA (lncRNA), has been suggested to serve as oncogene in multiple cancers. However, the functional involvement of OIP5-AS1 in oral squamous cell carcinoma (OSCC) was still unknown. The aims of this study were to investigate the functional role of OIP5-AS1 in OSCC and explore its potential mechanism. We found that OIP5-AS1 was up-regulated in OSCC tissues compared with adjacent non-tumor tissues. Loss-of-function experiments revealed that OIP5-AS1 knockdown significantly inhibited OSCC cell proliferation, migration and invasion in vitro, and retarded tumor growth in vivo. Mechanistically, OIP5-AS1 serves as a competing endogenous RNA of miR-338-3p and modulates the expression of neuropilin1 (NRP1), which has been identified as a downstream target gene of miR-338-3p in OSCC. Moreover, downregulation of miR-338-3p or overexpression of NRP1 partly reversed the inhibitory effect of OIP5-AS1 depletion on cell proliferation, migration and invasion. The current results provide evidences for the role of OIP5-AS1 in promoting OSCC progression by regulating miR-338-3p/NRP1 axis and suggest OIP5-AS1 as a potential therapy target for OSCC. 

Retracted: No

PubPeer comments:
 Fig 2C.  Cell ... colony formation were determined in SCC9 cells transfected with sh-NC and shOIP5-AS1.  Fig 2C from [Xu et al (2019)] .  Cell colony formation was determined in SO-RB-50 and Y79 cells transfected with sh-NC or sh-SNHG6. With 180-deg flipped versions below, to facilitate comparisons.  , 
 #1 Fig 2C again.  Now marked up for comparison with Figs 3C, 7D, 8B of [Han, Weng Cheng (2019)] .  , 
 *[left]* Fig 2(c),  Cell proliferation, migration, and invasion were determined in SGC7901 cells transfected with si-NC or si-SBF2-AS1 by ... wound healing . From  Long Noncoding RNASBF2-AS1 Promotes Gastric Cancer Progression via Regulating miR-545 EMS1 Axis ([He et al 2020] ). The panels previously appeared in *[right]* the present Fig 3A,  The cell migration was determined in SCC9 cells transfected with sh-NC and sh-OIP5- AS1 by wound healing assay . Some have been flipped through 180 deg, and different vertical lines have been drawn to delineate the scratch.  , 
 *[above]* Fig 3B,  The cell invasion was determined in SCC9 cells transfected with sh-NC and sh-OIP5-AS1 by transwell invasion assay . Panels were evidently sourced from *[below]* Fig 4h of  Long noncoding RNA MIR31HG inhibits hepatocellular carcinoma proliferation and metastasis by sponging microRNA-575 to modulate ST7L expression ([Yan et al 2018] ). Here flipped through 180 deg for ease of comparison.  ,