Up-regulation of miRNA-148a inhibits proliferation, invasion, and migration while promoting apoptosis of cervical cancer cells by down-regulating RRS1
Authors: Ying Zhang
Changhong Hao
Zonglan Xu
Zhengling Liu
Yonghui Tian
Lianbin Zhao
Bingmei Sun
Journal: Bioscience Reports (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: Cervical cancer; Invasion; Migration; Proliferation; RRS1
Affiliations: Department of Obstetrics and Gynecology, Linyi Central Hospital, Linyi 276400, Shandong Province, P.R. China., Department of Emergency, Linyi Central Hospital, Linyi 276400, Shandong Province, P.R. China., Department of Obstetrics and Gynecology, Linyi Central Hospital, Linyi 276400, Shandong Province, P.R. China .

Abstract:  The purpose of the present study is to figure out the role of miRNA-148a (miR-148a) in growth, apoptosis, invasion, and migration of cervical cancer cells by binding to regulator of ribosome synthesis 1 (RRS1). Cervical cancer and adjacent normal tissues, as well as cervical cancer cell line Caski, HeLa, C-33A, and normal cervical epithelial cell line H8 were obtained to detect the expression of miR-148a and RRS1. Relationship between miR-148a and RRS1 expression with clinicopathological characteristics was assessed. The selected Caski and HeLa cells were then transfected with miR-148a mimics, miR-148a inhibitors or RRS1 siRNA to investigate the role of miR-148a and RRS1 on proliferation, apoptosis, colony formation, invasion, and migration abilities of cervical cancer cells. Bioinformatics information and dual luciferase reporter gene assay was for used to detect the targetting relationship between miR-148a and RRS1. Down-regulated miR-148a and up-regulated RRS1 were found in cervical cancer tissues and cells. Down-regulated miR-148a and up-regulated RRS1 are closely related with prognostic factors of cervical cancer. RRS1 was determined as a target gene of miR-148a and miR-148a inhibited RRS1 expression in cervical cancer cells. Up-regulation of miR-148a inhibited cell proliferation, migration, and invasion while promoting apoptosis in Caski and HeLa cells. Our study suggests that miR-148a down-regulates RRS1 expression, thereby inhibiting the proliferation, migration, and invasion while promoting cell apoptosis of cervical cancer cells. 

Retracted: No

PubPeer comments:
 I have serious concerns about Figure 6A. When rotated two of the colonies have substantial overlap (outlined by like colored shapes, although position differs in at least one case), however the images are not identical suggesting that they may share the same source but have been edited. Could the authors comment? *** Closeup  *** Full figure  , 
 #1  , 
 Fig 10  , 
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 The Editorial Office has been made aware of potential issues surrounding the scientific validity of this paper, hence has issued an expression of concern to notify readers whilst the Editorial Office investigates. , 
 https: doi.org 10.1042 BSR-20181815_RET This Retraction follows an Expression of Concern relating to this article previously published by Portland Press. This article is being retracted from Bioscience Reports at the request of the Editor-in-Chief and the Editorial Board following receipt of a notification from a reader, alerting the Editorial Board to multiple manipulations in Figures 6A and 6C, as well as duplications in Figure 10A and 10C. The authors have been contacted in regard to the retraction, and have not responded to the Journal s queries and the concerns raised. Given the extent of the issues raised, Editorial Board stand by the decision to retract the article. ,