MiR-605-3p inhibits malignant progression of prostate cancer by up-regulating EZH2
Authors: Y-L Song
M-Z Pan
F Gao
Journal: European review for medical and pharmacological sciences (2019)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: microRNA-605-3p; prostate cancer; cell proliferation; invasion; migration
Affiliations: Department of Urology Surgery, The Seventh People's Hospital of Jinan, Jinan, China. .

Abstract:  Objective: This study aimed to investigate the effect of microRNA-605-3p (miR-605-3p) on cell proliferation, invasion and migration in prostate cancer cells, as well as its effects on the Enhancer Zeste Homolog 2 (EZH2) expression and the potential regulatory mechanisms. Patients and methods: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was applied to detect the miR-605-3p expression in 52 pairs of Prostate Cancer (PCa) tissues and normal tissues, and to analyze the relationship between miR-605-3p expression and PCa pathological parameters. MiR-605-3p mimics were transfected into PCa cell line PC-3 and DU145 to achieve miR-605-3p overexpression. Then, Cell Counting Kit-8 (CCK-8), cell colony formation assay and transwell invasion and migration assay were performed to analyze miR-605-3p's effect on the biological function of prostate cancer cells. Finally, the potential mechanisms of downstream genes were explored through bioinformatics analysis and recovery experiments. Results: We found that miR-605-3p expression in PCa was markedly lower than that in normal tissues. Patients with low miR-605-3p expression had higher tumor stage, higher incidence of lymph node metastasis and distant metastasis. In vitro analysis showed that miR-605-3p overexpression leads to a significant decrease in cell proliferation, invasion and migration ability. Subsequently, it was verified in cell line and tissue that EZH2 expression was remarkably increased in PCa, which was negatively correlated with miR-605-3p expression. In addition, the recovery experiment found that EZH2 can counteract the role of miR-605-3p in PCa, together they affected the malignant progression of PCa. Conclusions: MiR-605-3p was significantly associated with PCa stage, lymph node metastasis and distant metastasis and the poor prognosis; besides, it can inhibit the malignant progression of PCa. In addition, the study showed that miR-605-3p may inhibit the proliferation, invasion and migration ability of PCa by regulating EZH2. 

Retracted: Yes

PubPeer comments:
 Figure 2D and 4D. * Red boxes: Overlap between a panel in Figure 2D (invasion) and Figure 4D (migration).  , 
 *[left]* Fig 3C from [Pan, Song Gao (2019)] .  MiR-605-3p is significantly negatively correlated with the expression level of EZH2 in prostate cancer tissues . *[right]* Fig 3C from [Liu et al (2019)] .  A significant negative correlation was found between microRNA-106a expression and DAX-1 expression in BC tissues. Implausibly similar.  , 
 Fig 1D.  Kaplan-Meier survival curve of lung cancer patients based on FOXC2-AS1 expression .  Another manifestation of Hoya s Law for J.BUOn and ERMPS...  When 40 or 50 cases of any given cancer are dichotomised into half-groups of high- or low-expression of any given nc-RNA, survival rate for one half-group or both will drop to 0% at 75 months. Other examples, from left to right: - Fig 1 from [Zang et al (2020)] - Fig 1 from [Li et al (2020)] - Fig 1, and Fig 4D, from [Cui et al (2019)] - Fig 1D from [Gao et al (2019)] .  *[left]* Figure 1C from [Wu et al (2020)] . *[right]* Figs 1D, 3E from [Wu et al (2019)] .  , 
 #2 *[left]* Fig 4C from [Cui et al (2019)] .  qRT-PCR showed a significant negative correlation between HOXA-AS2 and miR216a-5p expression in breast cancer tissues . *[right]* Fig 3C from [Pan, Song Gao (2019)] .  MiR-605-3p is significantly negatively correlated with the expression level of EZH2 in prostate cancer tissues . More points in common than one would expect.  , 
 Further concerns over the validity of this paper are raised by looking at Table I. More than half of the patients with prostate cancer in this paper were women. It is quite remarkable.  , 
 Retracted [March 2021] . ------------------------------------------- We detected some serious inaccuracies and mistakes. Therefore, the article u201cMiR-605-3p inhibits malignant progression of prostate cancer by up-regulating EZH2, by M.-Z. Pan, Y.-L. Song, F. Gao, published in Eur Rev Med Pharmacol Sci 2019; 23 (20): 8795-8805u2013DOI: 10.26355 eurrev_201910_19274u2013PMID: 31696466u201d has been withdrawn. The Publisher apologizes for any inconvenience this may cause. ,