MiR-650 regulates the proliferation, migration and invasion of human oral cancer by targeting growth factor independent 1 (Gfi1)
Authors: Wu Chuanbin
Sun Libo
Zhou Qing
Sun Haijiang
Sun Ningning
Journal: Biochimie (2019)
Discipline: BIOLOGY & BIOCHEMISTRY
Keywords: Cell cycle arrest; Cell migration; Growth factor independent 1; MicroRNA; Oral cancer.
Affiliations: Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, 117 Nanjing North Street, Shenyang, 110002, Liaoning, China., Department of Oral and Maxillofacial Surgery, Southwest Medical University Affiliated Stomatological Hospital, Luzhou, Sichuan, 646000, China., Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, 117 Nanjing North Street, Shenyang, 110002, Liaoning, China. Electronic address: .

Abstract:  This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of the Editor-in-Chief. Following concerns raised by Dr. E. Bik and an anonymous reader, the journal conducted an investigation and found evidence that there had been improper manipulation and duplication of images in Figures 2A, 5C and 6C, as shown by partial overlap between images representing different experiments. An anonymous reader has also drawn attention to flow-cytometry data in Figure 1 similar to previously published data from a different laboratory (Med Sci Monit, 2018; 24: 5874-5880 DOI: 10.12659/MSM.909682, Figure 2B). After raising the complaint with the authors, the corresponding author agreed that the paper needed to be retracted. The authors apologise for any confusion that may have arisen from their article. 

Retracted: No

PubPeer comments:
 There appears to be some overlap between the images in figure 1 and figure 2, although they appear to be described differently. One example marked with red. Apologies if I have misunderstood. ***  *** Figure 1  *** Figure 2  , 
 The upper portion of the rightmost panel in Figure 2a appears to have repetitive markings similar to those created when using a clone tool. Could the authors provide original images? *** Contrast enhanced  , 
 The hard disk where the originals of these images was accidentally reformatted and they were lost; however, I keep originals of preliminary experiments as well as images of another experiment I did in 2017 with YPC4. These plates confirm the results shown in this paper. Preliminary experiment (from 2004) The originals picture were taken in tiff format, which apparently is not supported in your web page, therefore, I attach links to my dropbox account, where I happened to find these old pictures, that belong to an informal talk I gave to my lab mates. As you can see, although YPC4 was published in 2011 we had been working with it much earlier. Please, note that the best rescue of YPC4 phenotype was obtained with chimeric versions of AVP1 that had GFP attached at its N-terminus. These chimeras were generated a bit later and, therefore, are not included in this experiment. https: www.dropbox.com s 4r456376mx1llb7 YPgal.Tif?dl=0 https: www.dropbox.com s kyilo0anl5asl4r gal-pH7.Tif?dl=0 https: www.dropbox.com s 8cnp0vkqf6r2n3i gal-pH7%2C2.Tif?dl=0 https: www.dropbox.com s y0jla6b6cepit9e gal-pH7%2C4.Tif?dl=0 https: www.dropbox.com s 5pzihko2mdc04kh Glu-pH5.Tif?dl=0 https: www.dropbox.com s 60x61waaabywpi3 glu-pH7.Tif?dl=0 https: www.dropbox.com s k9d7j5bvfm9jls5 glu-pH7%2C2.Tif?dl=0 https: www.dropbox.com s 17ao3lmqwx2caap glu-pH7%2C4.Tif?dl=0 You can use the following figure as a guide to interpret the above attached figures:  Experiment done in 2017 to check the effect of Mg2+ on the complementation of vacuolar H+-ATPase by H+-PPases This experiment, that I personally did in 2017 demonstrates that this mutant, that was kept at -80 for more than a decade, maintained its robust phenotype of sensitivity towards pH, Ca2+ and Zn+2. The phenotype is completely rescued by the chimera THGAVP1, which is the name I give to TcGFPAVP1 in the lab. [file]      Again, you can use the following image to interpret these plates  , 
 #3 Dear Dr. Perez-Castineira, in one figure you provided u201cas a guide to interpretu201d linked data, three more yeast strips are duplicated (blue boxes) or duplicated and flipped vertically (orange boxes). Could you please comment?  , 
 #2 In addition to the repetitive markings in the upper portion of the rightmost panel in Figure 2A spotted by *Actinopolyspora biskrensis* in #2 (here highlighted by the magenta box), I spotted a clearly repeated portion of a panel yet in Figure 2A (blue boxes, contrast enhanced). Duplicated panels spotted in #1 are highlighted with red boxes:  ,