LDH-A promotes malignant behavior via activation of epithelial-to-mesenchymal transition in lung adenocarcinoma
Authors: Xiao-Jun Liu
Da Zhao
Shu-Qiao Yuan
Xiao-Ming Hou
Xin-An Wu
Journal: Bioscience reports (2019)
Discipline: MOLECULAR BIOLOGY & GENETICS
Keywords: EMT; LDH-A; LUAD; Prognosis; Tumor progression.
Affiliations: Department of Oncology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, People's Republic of China., The First Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu Province, People's Republic of China., Department of Medical Laboratory, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, People's Republic of China., The First Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu Province, People's Republic of China ., Department of Pharmacy, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, People's Republic of China.

Abstract:  Lactate dehydrogenase A (LDH-A) is a key enzyme during glycolysis, which increases the synthesis of related proteins and has elevated activity in cancer cells. The role of LDH-A in lung adenocarcinoma (LUAD) progression was investigated in the present study. Expression levels of LDH-A were assessed in LUAD samples, and the relationship between LDH-A expression status and the prognosis of LUAD patients was confirmed. The effect of LDH-A on proliferation, invasion, migration, and colony formation of cancer cells was assessed. We further determined the role of LDH-A in tumor growth in vivo by using xenograft LUAD tumor models. The potential mechanism of LDH-A promotion in LUAD progression was explored. LDH-A showed an abnormally high expression in LUAD, which is closely associated with poor prognosis in patients with LUAD. In in vitro experiments, silencing LDH-A expression in LUAD cells could effectively inhibit proliferation, invasion, migration, and colony formation of cancer cells. In in vivo experiments, tumor growth was markedly inhibited by LDH-A silencing in a xenograft model of LUAD. Notably, LDH-A could also promote tumor progression by regulating epithelial-mesenchymal transition (EMT)-related molecules. LDH-A can promote the malignant biological behaviors of LUAD cells, and thus can be a potential target for LUAD treatment. 

Retracted: No

PubPeer comments:
 Figure 3C may have a repeated image. Indicated with green boxes. *** Contrast enhanced closeup  ***  , 
 Thank you very much for your important reminder. After learning the news, we immediately checked the original data. I must admit that this is a low-level mistake. Due to carelessness in the typesetting of pictures, the above pictures are repeated. Here, I express my deep apologies. I will give you the corrected Figure 3C for your review, please refer to the figure below. We are happy to provide more detailed raw data. At the same time, we will actively contact the editor of the journal to talk about the formal submission of the corrected picture. We would like to express our apologies for any inconvenience caused to the readers of our published article by this error. , 
 Correction: https: portlandpress.com bioscirep article 40 6 BSR-20181476_COR 225511 Correction-LDH-A-promotes-malignant-behavior-via The authors of the original article above article u201cLDH-A promotes malignant behavior via activation of epithelial-to-mesenchymal transition in lung adenocarcinomau201d (Biosci Rep (2019) 39(1), https: doi.org 10.1042 BSR20181476) would like to correct Figure 3C, as the image of 0h for siRNA-1 and siRNA-2 due to mis-operation in visualisation. The authors declare that these corrections do not change the results or conclusions of their paper, and express their sincere apologies for any inconvenience that this error has caused to the readers. The corrected version of Figure 3 is presented here. (3C only)  ,