Syndecan-1 suppresses cell growth and migration via blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells
Authors: Shaojun Wang
Guimei Wang
Lipeng Jin
Xiaofei Zhang
Bin Cao
Hong Yang
Mingjuan Cui
Yongjun Mao
Journal: BMC cancer (2019)
Discipline: CLINICAL MEDICINE
Keywords: Colorectal carcinoma; JAK1/STAT3; Migration; Ras/Raf/MEK/ERK; Syndecan-1.
Affiliations: Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao, 266000, China., Department of Geriatrics, The Affiliated Hospital of Qingdao University, No.59 Haier Road, Laoshan District, Qingdao, 266000, Shandong, China., Emergency Department, The Affiliated Hospital of Qingdao University, Qingdao, 266000, China., Department of Geriatrics, The Affiliated Hospital of Qingdao University, No.59 Haier Road, Laoshan District, Qingdao, 266000, Shandong, China. .

Abstract:  Background: Syndecan-1 (SDC-1) is a crucial membrane proteoglycan, which is confirmed to participate in several tumor cell biological processes. However, the biological significance of SDC-1 in colorectal carcinoma is not yet clear. An objective of this study was to investigate the role of SDC-1 in colorectal carcinoma cells. Methods: Expression of SDC-1 in colorectal carcinoma tissues was evaluated by Reverse transcription-quantitative real-time PCR (RT-qPCR) and western blot. After transfection with pcDNA3.1 or pc-SDC-1, the transfection efficiency was measured. Next, SW480, SW620 and LOVO cell viability, apoptosis, migration and adhesion were assessed to explore the effects of exogenous overexpressed SDC-1 on colorectal carcinoma. In addition, the influences of aberrant expressed SDC-1 in Janus kinase 1 (JAK1)/signal transducer and activator of transcription 3 (STAT3) and rat sarcoma virus (Ras)/rapidly accelerated fibrosarcoma (Raf)/mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) pathways were detected by western blot analysis. Results: SDC-1 mRNA and protein levels were down-regulated in human colorectal carcinoma tissues. SDC-1 overexpression inhibited cell proliferation via suppressing CyclinD1 and c-Myc expression, meanwhile stimulated cell apoptosis via increasing the expression levels of B-cell lymphoma-2-associated x (Bax) and Cleaved-Caspase-3. Additionally, SDC-1 overexpression restrained cell migration via inhibiting the protein expression of matrix metallopeptidase 9 (MMP-9), and elicited cell adhesion through increasing intercellular cell adhesion molecule-1 (ICAM-1). Furthermore, SDC-1 overexpression suppressed JAK1/STAT3 and Ras/Raf/MEK/ERK-related protein levels. Conclusions: In general, the evidence from this study suggested that SDC-1 suppressed cell growth, migration through blocking JAK1/STAT3 and Ras/Raf/MEK/ERK pathways in human colorectal carcinoma cells. 

Retracted: No

PubPeer comments:
 Our investigation of falsified articles produced by Chinese papermills have so far uncovered over 300 articles. BMC Cancer is included in this list. The articles share the same structure (language, graphical illustrations and figures) and all Western immunoblots show background with repetitive patterns (seen by increasing the contrast). Here are examples from Figure 4 and 5 shown.  , 
 Peer review reports [here] . , 
 This paper belongs to a set of over 400 papers (as per February 2020) that share very similar Western blots with tadpole-like shaped bands, the same background pattern, and striking similarities in title structures, paper layout, bar graph design, and - in a subset - flow cytometry panels. Despite these similarities, these papers are authored by researchers from different departments and institutes, with almost no overlap in authors. The list of these papers is available here: https: docs.google.com spreadsheets d 1KXqTAyl4j-jVorFPMD2XRpr76LcIKJ0CVyIvRj0exYQ edit?usp=sharing A more detailed description of this set of papers, suspected to all be generated by a common source, a paper mill , can be found here: * https: forbetterscience.com 2020 01 24 the-full-service-paper-mill-and-its-chinese-customers (January 2020) * https: scienceintegritydigest.com 2020 02 21 the-tadpole-paper-mill (February 2020) The same paper mill has been described in these papers: * Jana Christopher - Systematic fabrication of scientific images revealed - FEBS Letters (2018) - https: febs.onlinelibrary.wiley.com doi full 10.1002 1873-3468.13201 * Jennifer A Byrne and Jana Christopher - Digital magic, or the dark arts of the 21st centuryu2014how can journals and peer reviewers detect manuscripts and publications from paper mills? - FEBS Letters (2020) - https: febs.onlinelibrary.wiley.com doi full 10.1002 1873-3468.13747 , 
 http: www.most.gov.cn zxgz kycxjs kycxgzdt 202201 t20220127_179206.html Google Translated Date: January 27, 2022 14:44 Source: Ministry of Science and Technology The paper  Syndecan-1 suppresses cell growth and migration via blocking JAK1 STAT3 and Ras Raf MEK ERK pathways in human colorectal carcinoma cells of Qingdao University Affiliated Hospital Mao Yongjun as the corresponding author and Wang Shaojun as the first author. After investigation, it was found that the paper was fraudulent and improperly signed. Mao Yongjun was unaware that the paper was signed. Wang Shaojun will be dealt with as follows: demerit for 1 year, cancel application or application for science and technology projects (special projects, funds, etc.), science and technology awards, and qualifications for science and technology talents for 5 years, cancel as nominee or recommender, nominee or recommender, evaluation expert Wait for the qualification for 5 years, record it in the file of scientific research integrity and dishonesty, give the scientific research integrity admonition talk, cancel the participation in this year s first evaluation and excellent evaluation, and the annual evaluation shall not be determined as qualified or above. (Notification link: https: kjc.qdu.edu.cn info 1023 3176.htm) ,