miR-335-5p targeting ICAM-1 inhibits invasion and metastasis of thyroid cancer cells
Authors: Qiu Zhang
Datong Deng
Jing Wu
Binshan Zha
Yong He
Linhong Hu
Changjiang Wang
Li Luo
Chunlin Zuo
Youmin Wang
Fang Dai
Mingwei Chen
Li Xia
Journal: Biomedicine & Pharmacotherapy (2018)
Discipline: PHARMACOLOGY & TOXICOLOGY
Keywords: ICAM-1; Thyroid cancer; miR-335-5p; miRNAs.
Affiliations: Department of Endocrinology, Institute of Endocrinology and Metabolism, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China., Department of Thyroid and Vascular Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China., Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China., Department of Endocrinology, Institute of Endocrinology and Metabolism, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China. Electronic address: .

Abstract:  miRNAs is a kind of noncoding small RNAs with negative regulation function. Some miRNAs play a crucial role in the growth of tumor cells. In this study, we analyzed the role of miR-335-5p and its target gene intercellular adhesion molecule 1 (ICAM-1) in thyroid cancer. Real-time polymerase chain reaction (PCR) results showed that the expression level of ICAM-1 in cancer tissues was higher than that in their adjacent tissues. The expression level of ICAM-1 in papillary thyroid carcinoma was also significantly higher than that in other types of tumors. However, the expression of miR-335-5p is opposite to that of ICAM-1. In human thyroid cancer cell lines TPC-1, FTC-133, TT and human thyroid follicular cell line Nthyori 3-1, the expression level of ICAM-1 in TPC-1 was significantly higher than that of other cells, while the expression level of miR-335-5p in TPC-1 was significantly lower than that of other cells. When ICAM-1 expression was downregulated and miR-335-5p expression was upregulated in TPC-1 cells, ICAM-1 expression was upregulated and miR-335-5p expression was downregulated in FTC-133 cells, we found that ICAM-1 could promote the proliferation of thyroid cancer cells, while miR-335-5p could inhibit the proliferation of thyroid cancer cells. miR-335-5p could combine with 3'UTR of ICAM-1 by bioinformatics prediction. Luciferase reporter gene analysis and Western blotting detection further confirmed that miR-335-5p could target ICAM-1 and inhibit its expression. The expression level of miR-335-5p was downregulated, while the expression level of ICAM-1 was upregulated in thyroid cancer. This study will help us better understand the pathogenesis of thyroid cancer and provide new insights into the treatment of this disease. 

Retracted: No

PubPeer comments:
 Fig 3.  Effects of miR-335-5p and ICAM-1 on the apoptosis of thyroid cancer cells. A. down regulation of ICAM-1 or up regulation of miR-335-5p in TPC-1 cells could promote the apoptosis of TPC-1 cells ... B. up regulation of ICAM-1 or inhibition of miR-335-5p in FTC-133 cells could inhibit the apoptosis of FTC-133 cells . Two panels are implausibly similar.  , 
 Fig 2.  C. Down regulation of ICAM-1 expression or over expression of miR-335-5p in TPC-1 cells could inhibit cell clone formation... D. Overexpression of ICAM-1 or inhibition of miR-335- 5p in FTC-133 cells could promote cell clone formation . Overlaps marked.  ,