Resveratrol inhibits matrix metalloproteinases to attenuate neuronal damage in cerebral ischemia: a molecular docking study exploring possible neuroprotection
Authors: Swet Chand Shukla
Anand Kumar Pandey
Pallab Bhattacharya
Sudip Paul
Ranjana Patnaik
Journal: Neural Regeneration Research (2015)
Discipline: NEUROSCIENCE & BEHAVIOR
Keywords: cerebral infarction; cerebral ischemia; extracellular matrix; matrix metalloproteinase; molecular docking
Affiliations: School of Biomedical Engineering, Indian Institute of Technology, Banaras Hindu University, Varanasi, India.

Abstract:  The main pathophysiology of cerebral ischemia is the structural alteration in the neurovascular unit, coinciding with neurovascular matrix degradation. Resveratrol has been reported to be one of the most potent chemopreventive agents that can inhibit cellular processes associated with ischemic stroke. Matrix metalloproteinases (MMPs) has been considered as a potential drug target for the treatment of cerebral ischemia. To explore this, we tried to investigate the interaction of resveratrol with MMPs through molecular docking studies. At 30 minutes before and 2 hours after cerebral ischemia/reperfusion induced by occlusion of the middle cerebral artery, 40 mg/kg resveratrol was intraperitoneally administered. After resveratrol administration, neurological function and brain edema were significantly alleviated, cerebral infarct volume was significantly reduced, and nitrite and malondialdehyde levels in the cortical and striatal regions were significantly decreased. The molecular docking study of resveratrol and MMPs revealed that resveratrol occupied the active site of MMP-2 and MMP-9. The binding energy of the complexes was -37.848672 kJ/mol and -36.6345 kJ/mol for MMP-2 and MMP-9, respectively. In case of MMP-2, Leu 164, Ala 165 and Thr 227 were engaged in H-Bonding with resveratrol and in case of MMP-9, H-bonding was found with Glu 402, Ala 417 and Arg 424 residues. These findings collectively reveal that resveratrol exhibits neuroprotective effects on cerebral ischemia through inhibiting MMP-2 and MMP-9 activity. 

Retracted: No

PubPeer comments:
 Figure 1 Effect of resveratrol on neurological deficit (A), cerebral infarct volume (B) and brain edema (C) following ischemic injury. Similar images of TTC staining from one of the author previous research article in Brain Research. Images background, dimensions and shade are altered. , 
 Images recycled from a previous publication of authors. Different treatment but similar images?  , 
 Retracted [ December 2022 ] . -------------------------------- Favorites Permissions NOTICE OF RETRACTION Retraction Resveratrol inhibits matrix metalloproteinases to attenuate neuronal damage in cerebral ischemia a molecular docking study exploring possible neuroprotection Author Information Neural Regeneration Research: December 2022 - Volume 17 - Issue 12 - p 2636 doi: 10.4103 1673-5374.310702 OPEN Metrics The Editors of Neural Regeneration Research (NRR) have retracted the publication entitled u201cResveratrol inhibits matrix metalloproteinases to attenuate neuronal damage in cerebral ischemia: a molecular docking study exploring possible neuroprotectionu201d (Pandey et al., 2015; doi: 10.4103 1673-5374.155429) following concerns raised around the seven subfigures presented in Figure 1B according to NRR Policy on Article Retraction. The subfigures of the upper panel in Figure 1B with the exception of the first subfigure and the subfigures of the lower panel in Figure 1B appear highly similar to or partly duplicate Figures 1 and 2 published in Brain Research (Pandey et al., 2011; doi: 10.1016 j.brainres.2021.147729). The authors stated that the similarities between panels within the article by Pandey et al. (2011) and their own article resulted from inadvertent mis-uploading of panels during figure preparation. They have offered replacement panels to address some of the issues outlined above. The authors provided raw data to support results in Figure 1B. However, the raw data provided were not sufficient to resolve the concerns noted above, including the similarities between panels from different experiments, time points, and groups. In light of these concerns, the Editor-in-Chief has serious concerns about the fact that some images in these studies were previously published in Brain Research (Pandey et al., 2011). The authors have a legal suit against the complaint. They disagree with the retraction. ,