PMC20201215pmc.key4822050CC BYno0010.1038/ncomms11197ncomms1119748220502704579911197This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/surname:Fang;given-names:Jiansurname:Cheng;given-names:Jingdongsurname:Gong;given-names:Zhousurname:Tang;given-names:Chunsurname:Wong;given-names:Jieminsurname:Yang;given-names:Huirongsurname:Cao;given-names:Chunyangsurname:Xu;given-names:Yanhuisurname:Wang;given-names:Jiaolongsurname:Zhang;given-names:Qiaosurname:Liu;given-names:Mengjiesurname:Gong;given-names:Ruisurname:Wang;given-names:Pingsurname:Zhang;given-names:Xiaodansurname:Feng;given-names:Yangyangsurname:Lan;given-names:WenxianTITLEfront720160Hemi-methylated DNA opens a closed conformation of UHRF1 to facilitate its histone recognitionchemicalCHEBI:cleaner02023-07-25T14:58:18ZHemi-methylated DNA0.999231protein_statecleaner02023-07-25T14:59:20ZDUMMY:closed0.99887425proteincleaner02023-07-25T14:58:00ZPR:UHRF1protein_typeMESH:cleaner02023-07-25T15:05:08ZhistoneABSTRACTabstract95UHRF1 is an important epigenetic regulator for maintenance DNA methylation. UHRF1 recognizes hemi-methylated DNA (hm-DNA) and trimethylation of histone H3K9 (H3K9me3), but the regulatory mechanism remains unknown. Here we show that UHRF1 adopts a closed conformation, in which a C-terminal region (Spacer) binds to the tandem Tudor domain (TTD) and inhibits H3K9me3 recognition, whereas the SET-and-RING-associated (SRA) domain binds to the plant homeodomain (PHD) and inhibits H3R2 recognition. Hm-DNA impairs the intramolecular interactions and promotes H3K9me3 recognition by TTD–PHD. The Spacer also facilitates UHRF1–DNMT1 interaction and enhances hm-DNA-binding affinity of the SRA. When TTD–PHD binds to H3K9me3, SRA-Spacer may exist in a dynamic equilibrium: either recognizes hm-DNA or recruits DNMT1 to chromatin. Our study reveals the mechanism for regulation of H3K9me3 and hm-DNA recognition by URHF1.0.9992975proteincleaner02023-07-25T14:58:01ZPR:UHRF1ptmMESH:cleaner02023-07-25T15:05:57Zmethylation0.9993113proteincleaner02023-07-25T14:58:01ZPR:UHRF10.97070986chemicalcleaner02023-07-25T14:58:17ZCHEBI:hemi-methylated DNA0.99714917chemicalcleaner02023-07-25T14:58:23ZCHEBI:hm-DNA0.98988324ptmcleaner02023-07-25T16:29:40ZMESH:trimethylation0.995805protein_typecleaner02023-07-25T15:05:08ZMESH:histoneprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:29:37ZH3residue_name_numberDUMMY:melaniev@ebi.ac.uk2023-08-08T12:29:31ZK9protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:30:01ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:30:15ZK9me30.9994023proteincleaner02023-07-25T14:58:01ZPR:UHRF10.9992417protein_statecleaner02023-07-25T14:59:19ZDUMMY:closed0.99918896structure_elementcleaner02023-07-25T14:56:57ZSO:C-terminal region0.9990657structure_elementcleaner02023-07-25T14:57:02ZSO:Spacerprotein_stateDUMMY:cleaner02023-07-25T15:13:03Zbinds to0.99929637structure_elementcleaner02023-07-25T14:57:11ZSO:tandem Tudor domain0.99940634structure_elementcleaner02023-07-25T14:57:16ZSO:TTDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:31:55ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:32:04ZK9me30.99883306structure_elementcleaner02023-07-25T14:57:27ZSO:SET-and-RING-associated0.9949174structure_elementcleaner02023-07-25T14:57:33ZSO:SRAprotein_stateDUMMY:cleaner02023-07-25T15:13:03Zbinds to0.9913373structure_elementcleaner02023-07-25T14:57:42ZSO:plant homeodomain0.99932337structure_elementcleaner02023-07-25T14:57:48ZSO:PHD0.87803274sitecleaner02023-07-25T15:03:31ZSO:H3R20.9954868chemicalcleaner02023-07-25T14:58:24ZCHEBI:Hm-DNAprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:30:55ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:31:41ZK9me3structure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHD0.9989747structure_elementcleaner02023-07-25T14:57:03ZSO:Spacercomplex_assemblyGO:cleaner02023-07-25T16:03:29ZUHRF1–DNMT1evidenceDUMMY:cleaner02023-07-25T14:56:50Zhm-DNA-binding affinity0.99947935structure_elementcleaner02023-07-25T14:57:34ZSO:SRAstructure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHDprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds toprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:32:18ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:32:29ZK9me3structure_elementSO:cleaner02023-07-25T15:04:02ZSRA-Spacer0.9976597chemicalcleaner02023-07-25T14:58:24ZCHEBI:hm-DNA0.99921703proteincleaner02023-07-25T15:00:38ZPR:DNMT1protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:32:46ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:32:56ZK9me30.99476165chemicalcleaner02023-07-25T14:58:24ZCHEBI:hm-DNA0.9994437proteincleaner02023-07-25T16:29:03ZPR:URHF1ABSTRACTabstract1016 UHRF1 is involved in the maintenance of DNA methylation, but the regulatory mechanism of this epigenetic regulator is unclear. Here, the authors show that it has a closed conformation and are able to make conclusions about the mechanism of recognition of epigenetic marks.0.9987054proteincleaner02023-07-25T14:58:01ZPR:UHRF1ptmMESH:cleaner02023-07-25T15:05:57Zmethylation0.99924755protein_statecleaner02023-07-25T14:59:20ZDUMMY:closedINTROparagraph1290DNA methylation is an important epigenetic modification for gene repression, X-chromosome inactivation, genome imprinting and maintenance of genome stability. Mammalian DNA methylation is established by de novo DNA methyltransferases DNMT3A/3B, and DNA methylation patterns are maintained by maintenance DNA methyltransferase 1 (DNMT1) during DNA replication. Ubiquitin-like, containing PHD and RING fingers domains, 1 (UHRF1, also known as ICBP90 and NP95 in mouse) was shown to be essential for maintenance DNA methylation through recruiting DNMT1 to replication forks in S phase of the cell cycle. UHRF1 is essential for S phase entry and is involved in heterochromatin formation. UHRF1 also plays an important role in promoting proliferation and is shown to be upregulated in a number of cancers, suggesting that UHRF1 may serve as a potential drug target for therapeutic applications.ptmMESH:cleaner02023-07-25T15:05:57Zmethylation0.9980562taxonomy_domaincleaner02023-07-25T15:00:03ZDUMMY:MammalianptmMESH:cleaner02023-07-25T15:05:57Zmethylation0.9966591protein_typecleaner02023-07-25T15:00:19ZMESH:DNA methyltransferases0.9978175proteincleaner02023-07-25T15:00:24ZPR:DNMT3A/3BptmMESH:cleaner02023-07-25T15:05:57Zmethylation0.9750573proteincleaner02023-07-25T15:00:32ZPR:DNA methyltransferase 10.99899286proteincleaner02023-07-25T15:00:36ZPR:DNMT1proteinPR:cleaner02023-07-25T14:59:44ZUbiquitin-like, containing PHD and RING fingers domains, 10.999151proteincleaner02023-07-25T14:58:01ZPR:UHRF10.9991623proteincleaner02023-07-25T15:00:45ZPR:ICBP900.9990305proteincleaner02023-07-25T15:00:49ZPR:NP950.99646413taxonomy_domaincleaner02023-07-25T15:00:55ZDUMMY:mouseptmMESH:cleaner02023-07-25T15:05:57Zmethylation0.9990791proteincleaner02023-07-25T15:00:38ZPR:DNMT10.99863005proteincleaner02023-07-25T14:58:01ZPR:UHRF10.998461proteincleaner02023-07-25T14:58:01ZPR:UHRF10.9986771proteincleaner02023-07-25T14:58:01ZPR:UHRF1INTROparagraph2180UHRF1 is a multi-domain containing protein connecting histone modification and DNA methylation. As shown in Fig. 1a, UHRF1 is comprised of an N-terminal ubiquitin-like domain, followed by a tandem Tudor domain (TTD containing TTDN and TTDC sub-domains), a plant homeodomain (PHD), a SET-and-RING-associated (SRA) domain, and a C-terminal really interesting new gene (RING) domain. We and other groups demonstrated that the TTD and the PHD coordinately recognize histone H3K9me3, in which residue R2 is recognized by the PHD and tri-methylation of residue K9 (K9me3) is recognized by the TTD. The SRA preferentially binds to hemi-methylated DNA (hm-DNA). Recent studies show that the SRA directly binds to replication focus targeting sequence (RFTS) of DNMT1 (RFTSDNMT1). A spacer region (Fig. 1a, designated Spacer hereafter) connecting the SRA and the RING is rich in basic residues and predicted to be unstructured for unknown function. Recent study shows that phosphatidylinostiol phosphate PI5P binds to the Spacer and induces a conformational change of UHRF1 to allow the TTD to recognize H3K9me3 (ref.). These studies indicate that UHRF1 connects dynamic regulation of DNA methylation and H3K9me3, which are positively correlated in human genome. However, how UHRF1 regulates the recognition of these two repressive epigenetic marks and recruits DNMT1 for chromatin localization remain largely unknown.0.99919814proteincleaner02023-07-25T14:58:01ZPR:UHRF1protein_typeMESH:cleaner02023-07-25T15:05:08ZhistonechemicalCHEBI:cleaner02023-07-25T15:05:41ZDNAptmMESH:cleaner02023-07-25T15:05:51Zmethylation0.9992995proteincleaner02023-07-25T14:58:01ZPR:UHRF10.99908644structure_elementcleaner02023-07-25T16:31:03ZSO:ubiquitin-like domain0.99913794structure_elementcleaner02023-07-25T14:57:12ZSO:tandem Tudor domain0.999468structure_elementcleaner02023-07-25T14:57:17ZSO:TTD0.998676structure_elementcleaner02023-07-25T16:31:07ZSO:TTDN0.9981198structure_elementcleaner02023-07-25T16:31:10ZSO:TTDC0.9990694structure_elementcleaner02023-07-25T14:57:43ZSO:plant homeodomain0.99924564structure_elementcleaner02023-07-25T14:57:49ZSO:PHD0.9987116structure_elementcleaner02023-07-25T14:57:28ZSO:SET-and-RING-associated0.9961398structure_elementcleaner02023-07-25T14:57:34ZSO:SRA0.9305976structure_elementcleaner02023-07-25T15:01:22ZSO:really interesting new gene0.9275266structure_elementcleaner02023-07-25T15:01:26ZSO:RING0.9994217structure_elementcleaner02023-07-25T14:57:17ZSO:TTD0.9994911structure_elementcleaner02023-07-25T14:57:49ZSO:PHD0.9925216protein_typecleaner02023-07-25T15:05:07ZMESH:histoneprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:33:20ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:33:29ZK9me30.8370888residue_name_numbercleaner02023-07-25T15:06:48ZDUMMY:R20.99943274structure_elementcleaner02023-07-25T14:57:49ZSO:PHD0.9749963ptmcleaner02023-07-25T15:06:10ZMESH:tri-methylation0.9221057residue_name_numbercleaner02023-07-25T15:06:25ZDUMMY:K90.9694169ptmcleaner02023-07-25T16:29:47ZMESH:K9me30.9994722structure_elementcleaner02023-07-25T14:57:17ZSO:TTD0.9991067structure_elementcleaner02023-07-25T14:57:34ZSO:SRAprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds tochemicalCHEBI:cleaner02023-07-25T14:58:18Zhemi-methylated DNA0.9955127chemicalcleaner02023-07-25T14:58:24ZCHEBI:hm-DNA0.99911934structure_elementcleaner02023-07-25T14:57:34ZSO:SRAprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.6492692structure_elementcleaner02023-07-25T15:02:17ZSO:replication focus targeting sequence0.557462structure_elementcleaner02023-07-25T15:02:43ZSO:RFTS0.9992735proteincleaner02023-07-25T15:00:38ZPR:DNMT10.9992048proteincleaner02023-07-25T16:02:12ZPR:RFTSDNMT10.99836123structure_elementcleaner02023-07-25T15:01:57ZSO:spacer region0.9973368structure_elementcleaner02023-07-25T14:57:03ZSO:Spacer0.9995253structure_elementcleaner02023-07-25T14:57:34ZSO:SRA0.9995208structure_elementcleaner02023-07-25T15:01:28ZSO:RING0.91302955protein_statecleaner02023-07-25T16:47:49ZDUMMY:unstructured0.99880505chemicalcleaner02023-07-25T15:02:53ZCHEBI:phosphatidylinostiol phosphate0.998345chemicalcleaner02023-07-25T15:03:00ZCHEBI:PI5Pprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.99899036structure_elementcleaner02023-07-25T14:57:03ZSO:Spacer0.99934953proteincleaner02023-07-25T14:58:01ZPR:UHRF10.99953246structure_elementcleaner02023-07-25T14:57:17ZSO:TTDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:34:54ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:35:03ZK9me30.99926096proteincleaner02023-07-25T14:58:01ZPR:UHRF1chemicalCHEBI:melaniev@ebi.ac.uk2023-08-08T12:34:37ZDNAptmMESH:cleaner02023-07-25T15:05:57Zmethylationprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:34:14ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:34:25ZK9me30.9982955speciescleaner02023-07-25T16:39:10ZMESH:human0.9993181proteincleaner02023-07-25T14:58:01ZPR:UHRF10.9992186proteincleaner02023-07-25T15:00:38ZPR:DNMT1INTROparagraph3589Here we report that UHRF1 adopts a closed conformation, in which the C-terminal Spacer binds to the TTD and inhibits its recognition of H3K9me3, whereas the SRA binds to the PHD and inhibits its recognition of H3R2 (unmethylated histone H3 at residue R2). Upon binding to hm-DNA, UHRF1 impairs the intramolecular interactions and promotes the H3K9me3 recognition by TTD–PHD, which may further enhance its genomic localization. As a result, UHRF1 is locked in the open conformation by the association of H3K9me3 by TTD–PHD, and thus SRA-Spacer either recognizes hm-DNA or recruits DNMT1 for DNA methylation. Therefore, UHRF1 may engage in a sophisticated regulation for its chromatin localization and recruitment of DNMT1 through a mechanism yet to be fully elucidated. Our study reveals the mechanism for regulation of H3K9me3 and hm-DNA recognition by UHRF1.0.99936396proteincleaner02023-07-25T14:58:01ZPR:UHRF10.9992605protein_statecleaner02023-07-25T14:59:20ZDUMMY:closedstructure_elementSO:cleaner02023-07-25T14:57:03ZSpacerprotein_stateDUMMY:cleaner02023-07-25T15:13:03Zbinds to0.99956805structure_elementcleaner02023-07-25T14:57:17ZSO:TTDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:35:18ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:35:26ZK9me30.9994542structure_elementcleaner02023-07-25T14:57:34ZSO:SRAprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.9995419structure_elementcleaner02023-07-25T14:57:49ZSO:PHD0.79977167sitecleaner02023-07-25T15:03:30ZSO:H3R20.99909306protein_statecleaner02023-07-25T15:04:47ZDUMMY:unmethylatedprotein_typeMESH:cleaner02023-07-25T15:05:08Zhistoneprotein_typeMESH:cleaner02023-07-25T15:05:25ZH30.48252225residue_name_numbercleaner02023-07-25T15:06:49ZDUMMY:R2protein_stateDUMMY:cleaner02023-07-25T15:04:36Zbinding to0.9958095chemicalcleaner02023-07-25T14:58:24ZCHEBI:hm-DNA0.9993137proteincleaner02023-07-25T14:58:01ZPR:UHRF1protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:35:40ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:35:49ZK9me3structure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHD0.9993806proteincleaner02023-07-25T14:58:01ZPR:UHRF10.9991967protein_statecleaner02023-07-25T16:47:52ZDUMMY:openprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:36:02ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:36:11ZK9me3structure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHD0.9654761structure_elementcleaner02023-07-25T15:04:01ZSO:SRA-Spacer0.99189776chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.99909675proteincleaner02023-07-25T15:00:38ZPR:DNMT1chemicalCHEBI:cleaner02023-07-25T15:07:18ZDNAptmMESH:cleaner02023-07-25T15:05:57Zmethylation0.99935514proteincleaner02023-07-25T14:58:01ZPR:UHRF10.99914134proteincleaner02023-07-25T15:00:38ZPR:DNMT1protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:36:27ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:36:35ZK9me30.9882726chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.99934787proteincleaner02023-07-25T14:58:01ZPR:UHRF1RESULTStitle_14453ResultsRESULTStitle_24461Hm-DNA facilitates histone H3K9me3 recognition by UHRF10.9845609chemicalcleaner02023-07-25T14:58:25ZCHEBI:Hm-DNA0.98075485protein_typecleaner02023-07-25T15:05:08ZMESH:histoneprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:36:54ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:37:04ZK9me30.9989887proteincleaner02023-07-25T14:58:01ZPR:UHRF1RESULTSparagraph4517To investigate how UHRF1 coordinates the recognition of H3K9me3 and hm-DNA, we purified recombinant UHRF1 (truncations and mutations) proteins from bacteria. We first performed an in vitro pull-down assay using biotinylated histone H3 peptides and hm-DNA (Supplementary Table 1). As shown in Fig. 1b, hm-DNA largely enhanced the interaction between full-length UHRF1 and unmethylated histone H3 (H3K9me0) or H3K9me3 peptide. Compared with hm-DNA, um-DNA (unmethylated DNA) or fm-DNA (fully methylated DNA) showed marginal effect on facilitating the interaction between UHRF1 and histone peptides, which is consistent with previous studies that UHRF1 prefers hm-DNA for chromatin association (Supplementary Fig. 1a). In contrast, histone peptides showed no enhancement on the interaction between hm-DNA and UHRF1 (Fig. 1c). These results suggest that hm-DNA facilitates histone recognition by UHRF1.0.9990995proteincleaner02023-07-25T14:58:01ZPR:UHRF1protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:37:24ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:37:32ZK9me30.9977552chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.99919635proteincleaner02023-07-25T14:58:01ZPR:UHRF10.9987502experimental_methodcleaner02023-07-25T16:39:17ZMESH:in vitro pull-down assay0.7566109protein_statecleaner02023-07-25T16:30:09ZDUMMY:biotinylated0.8355774protein_typecleaner02023-07-25T15:05:08ZMESH:histone0.8583309protein_typecleaner02023-07-25T15:07:50ZMESH:H30.9979067chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.9975527chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.999162protein_statecleaner02023-07-25T15:09:00ZDUMMY:full-length0.99920505proteincleaner02023-07-25T14:58:01ZPR:UHRF10.99908483protein_statecleaner02023-07-25T15:04:48ZDUMMY:unmethylated0.94932944protein_typecleaner02023-07-25T15:05:08ZMESH:histone0.8987189protein_typecleaner02023-07-25T15:07:49ZMESH:H3protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:37:47ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:37:57ZK9me0protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:38:09ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:38:18ZK9me30.99857134chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.9987547chemicalcleaner02023-07-25T15:08:28ZCHEBI:um-DNA0.99885714protein_statecleaner02023-07-25T15:04:48ZDUMMY:unmethylated0.9731914chemicalcleaner02023-07-25T16:30:14ZCHEBI:DNA0.99861336chemicalcleaner02023-07-25T15:08:22ZCHEBI:fm-DNA0.9987992protein_statecleaner02023-07-25T15:08:14ZDUMMY:fully methylated0.9773302chemicalcleaner02023-07-25T16:30:18ZCHEBI:DNA0.9990351proteincleaner02023-07-25T14:58:01ZPR:UHRF1protein_typeMESH:cleaner02023-07-25T15:05:08Zhistone0.9991819proteincleaner02023-07-25T14:58:01ZPR:UHRF10.99527615chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNAprotein_typeMESH:cleaner02023-07-25T15:05:08Zhistone0.9965648chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.9990885proteincleaner02023-07-25T14:58:01ZPR:UHRF10.99166584chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNAprotein_typeMESH:cleaner02023-07-25T15:05:08Zhistone0.99913424proteincleaner02023-07-25T14:58:01ZPR:UHRF1RESULTSparagraph5416Our previous studies show that the PHD recognizes H3K9me0 and the TTD and the PHD together (TTD–PHD) coordinately recognize H3K9me3 (refs.). We noticed that the isolated TTD–PHD showed much higher (∼31-fold) binding affinity to H3K9me3 peptide than full-length UHRF1 (Fig. 1d and Supplementary Table 2), and the isolated PHD showed much higher (∼34-fold) binding affinity to H3K9me0 peptide than full-length UHRF1 (Fig. 1e). The gel filtration analysis showed that UHRF1 is a monomer in solution (Supplementary Fig. 1b), indicating that the intramolecular (not intermolecular) interaction of UHRF1 regulates histone recognition. These results suggest that UHRF1 adopts an unfavourable conformation for histone H3 tails recognition, in which TTD–PHD might be blocked by other regions of UHRF1, and hm-DNA impairs this intramolecular interaction to facilitate its recognition of histone H3 tails.0.99949586structure_elementcleaner02023-07-25T14:57:49ZSO:PHDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:38:33ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:38:43ZK9me00.99951327structure_elementcleaner02023-07-25T14:57:17ZSO:TTD0.9994549structure_elementcleaner02023-07-25T14:57:49ZSO:PHDstructure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:38:56ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:39:10ZK9me3protein_stateDUMMY:cleaner02023-07-25T15:16:09Zisolatedstructure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHD0.99869025evidencecleaner02023-07-25T15:10:17ZDUMMY:binding affinityprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:39:22ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:39:31ZK9me30.9990852protein_statecleaner02023-07-25T15:08:59ZDUMMY:full-length0.99931014proteincleaner02023-07-25T14:58:01ZPR:UHRF10.9994672structure_elementcleaner02023-07-25T14:57:49ZSO:PHD0.9987298evidencecleaner02023-07-25T15:10:17ZDUMMY:binding affinityprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:39:44ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:39:53ZK9me00.99909943protein_statecleaner02023-07-25T15:09:00ZDUMMY:full-length0.9993193proteincleaner02023-07-25T14:58:01ZPR:UHRF10.99865484experimental_methodcleaner02023-07-25T16:39:20ZMESH:gel filtration analysis0.9992993proteincleaner02023-07-25T14:58:01ZPR:UHRF10.998701oligomeric_statecleaner02023-07-25T16:39:02ZDUMMY:monomer0.9992415proteincleaner02023-07-25T14:58:01ZPR:UHRF1protein_typeMESH:cleaner02023-07-25T15:05:08Zhistone0.99931514proteincleaner02023-07-25T14:58:01ZPR:UHRF1protein_typeMESH:cleaner02023-07-25T15:05:08Zhistone0.42043397protein_typecleaner02023-07-25T15:07:50ZMESH:H3structure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHD0.9992786proteincleaner02023-07-25T14:58:01ZPR:UHRF10.9796707chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.69733983protein_typecleaner02023-07-25T15:05:08ZMESH:histone0.5546273protein_typecleaner02023-07-25T15:07:50ZMESH:H3RESULTStitle_26321Intramolecular interaction within UHRF10.9989747proteincleaner02023-07-25T14:58:01ZPR:UHRF1RESULTSparagraph6361To test above hypothesis, we performed glutathione S-transferase (GST) pull-down assay using various truncations of UHRF1. Interestingly, the TTD directly bound to SRA-Spacer but not the SRA, suggesting that the Spacer (residues 587–674) is important for the intramolecular interaction (Fig. 2a). The isothermal titration calorimetry (ITC) measurements show that the TTD bound to the Spacer (but not the SRA) in a 1:1 stoichiometry with a binding affinity (KD) of 1.59 μM (Fig. 2b). The presence of the Spacer markedly impaired the interaction between TTD–PHD and H3K9me3 (Fig. 2c). The results indicate that the Spacer directly binds to the TTD and inhibits its interaction with H3K9me3.experimental_methodMESH:cleaner02023-07-25T16:36:22Zglutathione S-transferase (GST) pull-down assay0.8383519experimental_methodcleaner02023-07-25T16:39:24ZMESH:truncations0.99936134proteincleaner02023-07-25T14:58:01ZPR:UHRF10.99953187structure_elementcleaner02023-07-25T14:57:17ZSO:TTDprotein_stateDUMMY:cleaner02023-07-25T15:10:32Zbound tostructure_elementSO:cleaner02023-07-25T15:04:02ZSRA-Spacer0.9419889structure_elementcleaner02023-07-25T14:57:34ZSO:SRA0.99881434structure_elementcleaner02023-07-25T14:57:03ZSO:Spacer0.9978974residue_rangecleaner02023-07-25T16:35:57ZDUMMY:587–6740.99897915experimental_methodcleaner02023-07-25T16:36:28ZMESH:isothermal titration calorimetry0.9986539experimental_methodcleaner02023-07-25T16:36:31ZMESH:ITC0.99951494structure_elementcleaner02023-07-25T14:57:17ZSO:TTD0.9987566protein_statecleaner02023-07-25T15:10:31ZDUMMY:bound to0.99883133structure_elementcleaner02023-07-25T14:57:03ZSO:Spacer0.57471496structure_elementcleaner02023-07-25T14:57:34ZSO:SRA0.9984056evidencecleaner02023-07-25T15:10:16ZDUMMY:binding affinity0.99863535evidencecleaner02023-07-25T15:10:10ZDUMMY:KD0.9984523protein_statecleaner02023-07-25T15:10:24ZDUMMY:presence of0.9855452structure_elementcleaner02023-07-25T14:57:03ZSO:Spacerstructure_elementSO:cleaner02023-07-25T15:11:20ZTTD–PHDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:40:09ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:40:20ZK9me30.9973073structure_elementcleaner02023-07-25T14:57:03ZSO:Spacerprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.99943787structure_elementcleaner02023-07-25T14:57:17ZSO:TTDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:40:32ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:40:42ZK9me3RESULTSparagraph7057The GST pull-down assay also shows that the PHD bound to the SRA, which was further confirmed by the ITC measurements (KD=26.7 μM; Fig. 2a,d). Compared with the PHD alone, PHD-SRA showed decreased binding affinity to H3K9me0 peptide by a factor of eight (Fig. 2e). Pre-incubation of the SRA also modestly impaired PHD–H3K9me0 interaction. These results indicate that the SRA directly binds to the PHD and inhibits its binding affinity to H3K9me0. Taken together, UHRF1 seems to adopt a closed form through intramolecular interactions (TTD–Spacer and PHD-SRA), which inhibit histone H3 tail recognition by UHRF1.0.9989052experimental_methodcleaner02023-07-25T16:36:39ZMESH:GST pull-down assay0.99954224structure_elementcleaner02023-07-25T14:57:49ZSO:PHD0.9989557protein_statecleaner02023-07-25T15:10:32ZDUMMY:bound to0.98258084structure_elementcleaner02023-07-25T14:57:34ZSO:SRA0.9976513experimental_methodcleaner02023-07-25T16:39:30ZMESH:ITC0.99855214evidencecleaner02023-07-25T15:10:11ZDUMMY:KD0.9994531structure_elementcleaner02023-07-25T14:57:49ZSO:PHD0.9981786protein_statecleaner02023-07-25T16:47:57ZDUMMY:alone0.94781685structure_elementcleaner02023-07-25T15:16:50ZSO:PHD-SRA0.99865395evidencecleaner02023-07-25T15:10:17ZDUMMY:binding affinityprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:40:54ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:41:03ZK9me00.99731904experimental_methodcleaner02023-07-25T16:39:33ZMESH:Pre-incubation0.48420557structure_elementcleaner02023-07-25T14:57:34ZSO:SRA0.9934504structure_elementcleaner02023-07-25T14:57:49ZSO:PHDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:41:14ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:41:25ZK9me00.9809187structure_elementcleaner02023-07-25T14:57:34ZSO:SRAprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.9994642structure_elementcleaner02023-07-25T14:57:49ZSO:PHD0.9982419evidencecleaner02023-07-25T15:10:17ZDUMMY:binding affinityprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:41:40ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:41:49ZK9me00.9993193proteincleaner02023-07-25T14:58:01ZPR:UHRF10.9992454protein_statecleaner02023-07-25T14:59:20ZDUMMY:closedstructure_elementSO:cleaner02023-07-25T15:17:20ZTTD–Spacerstructure_elementSO:cleaner02023-07-25T15:16:51ZPHD-SRAprotein_typeMESH:cleaner02023-07-25T15:05:08Zhistoneprotein_typeMESH:cleaner02023-07-25T15:07:50ZH30.9993266proteincleaner02023-07-25T14:58:01ZPR:UHRF1RESULTStitle_27676Overall structure of TTD–Spacer0.9973246evidencecleaner02023-07-25T16:44:33ZDUMMY:structure0.99570864structure_elementcleaner02023-07-25T15:17:21ZSO:TTD–SpacerRESULTSparagraph7710To investigate the intramolecular interaction within UHRF1, we first mapped the minimal regions within the Spacer for the interaction with the TTD (Supplementary Fig. 2a). Internal deletions of the Spacer, including SpacerΔ660–664, SpacerΔ665–669, SpacerΔ670–674 and Spacer642–674, bound to the TTD with comparable binding affinities to that of the Spacer, whereas Spacer587–641 showed no detectable interaction. SpacerΔ642–651, SpacerΔ650–654 and SpacerΔ655–659 also decreased binding affinities, indicating that residues 642–674 are important for TTD–Spacer interaction.0.99916494proteincleaner02023-07-25T14:58:02ZPR:UHRF10.99835926structure_elementcleaner02023-07-25T14:57:03ZSO:Spacer0.9993787structure_elementcleaner02023-07-25T14:57:17ZSO:TTD0.9698804experimental_methodcleaner02023-07-25T16:39:36ZMESH:deletions0.99842167structure_elementcleaner02023-07-25T14:57:03ZSO:SpacermutantMESH:cleaner02023-07-25T15:18:22ZSpacerΔ660–664mutantMESH:cleaner02023-07-25T15:18:42ZSpacerΔ665–669mutantMESH:cleaner02023-07-25T15:19:03ZSpacerΔ670–674mutantMESH:cleaner02023-07-25T15:19:23ZSpacer642–6740.99854124protein_statecleaner02023-07-25T15:10:32ZDUMMY:bound to0.99894804structure_elementcleaner02023-07-25T14:57:17ZSO:TTD0.9986196evidencecleaner02023-07-25T16:44:38ZDUMMY:binding affinities0.99589765structure_elementcleaner02023-07-25T14:57:03ZSO:SpacermutantMESH:cleaner02023-07-25T15:21:58ZSpacer587–641mutantMESH:cleaner02023-07-25T15:19:44ZSpacerΔ642–651mutantMESH:cleaner02023-07-25T15:20:05ZSpacerΔ650–654mutantMESH:cleaner02023-07-25T15:20:29ZSpacerΔ655–6590.9985037evidencecleaner02023-07-25T16:44:42ZDUMMY:binding affinities0.99734336residue_rangecleaner02023-07-25T15:20:40ZDUMMY:642–674structure_elementSO:cleaner02023-07-25T15:17:21ZTTD–SpacerRESULTSparagraph8309We next determined the solution structure of the TTD (residues 134–285) bound to Spacer627–674 by conventional NMR techniques (Supplementary Table 3 and Supplementary Fig. 3a,b). In the complex structure, each Tudor domain adopts a ‘Royal' fold containing a characteristic five-stranded β-sheet and the two Tudor domains tightly pack against each other with a buried area of 573 Å2 (Fig. 3a). The TTD adopts similar fold to that in TTD–PHD–H3K9me3 complex structure (PDB: 4GY5) with a root-mean-square deviation of 1.09 Å for 128 Cα atoms, indicating that the Spacer does not result in obvious conformational change of the TTD (Fig. 3b). The Spacer (residues 643–655 were built in the model) adopts an extended conformation and binds to an acidic groove on the TTD (Fig. 3c).0.9957992evidencecleaner02023-07-25T16:44:45ZDUMMY:solution structure0.9992454structure_elementcleaner02023-07-25T14:57:17ZSO:TTD0.99801356residue_rangecleaner02023-07-25T15:20:43ZDUMMY:134–2850.99913573protein_statecleaner02023-07-25T15:10:32ZDUMMY:bound to0.98736215residue_rangecleaner02023-07-25T15:24:25ZDUMMY:Spacer627–6740.9983102experimental_methodcleaner02023-07-25T16:39:42ZMESH:NMR0.9735048evidencecleaner02023-07-25T16:44:48ZDUMMY:complex structure0.9985361structure_elementcleaner02023-07-25T16:31:14ZSO:Tudor domainstructure_elementSO:cleaner02023-07-25T16:31:40Z‘Royal' fold0.9976389structure_elementcleaner02023-07-25T16:31:45ZSO:five-stranded β-sheet0.99847436structure_elementcleaner02023-07-25T16:31:48ZSO:Tudor domains0.99931407structure_elementcleaner02023-07-25T14:57:17ZSO:TTD0.99921435complex_assemblycleaner02023-07-25T15:22:28ZGO:TTD–PHD–H3K9me30.7332863evidencecleaner02023-07-25T16:44:50ZDUMMY:structure0.9981439evidencecleaner02023-07-25T16:44:53ZDUMMY:root-mean-square deviation0.9938008structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.99923444structure_elementcleaner02023-07-25T14:57:17ZSO:TTD0.9991209structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.9979308residue_rangecleaner02023-07-25T15:24:36ZDUMMY:643–6550.98440146protein_statecleaner02023-07-25T16:48:08ZDUMMY:extended conformationprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.9988172sitecleaner02023-07-25T16:51:32ZSO:acidic groove0.9993272structure_elementcleaner02023-07-25T14:57:18ZSO:TTDRESULTSparagraph9105The TTD–Spacer interaction is mediated by a number of hydrogen bonds (Fig. 3d). The side chain of residue K648 forms hydrogen bonds with the carbonyl oxygen atom of D189 and side chain of D190 of the TTD. The side chain of residue R649 packs against an acidic surface mainly formed by residues D142 and E153. Residue S651 forms hydrogen bonds with the main chain of residues G236 and W238. The interaction is further supported by hydrogen bonds formed between residues K650, A652, G653 and G654 of the Spacer and residues N228, G236 and W238 of the TTD, respectively.structure_elementSO:cleaner02023-07-25T15:17:21ZTTD–Spacer0.99648bond_interactioncleaner02023-07-25T15:24:58ZMESH:hydrogen bonds0.9994708residue_name_numbercleaner02023-07-25T15:25:15ZDUMMY:K6480.99632293bond_interactioncleaner02023-07-25T15:24:59ZMESH:hydrogen bonds0.99950325residue_name_numbercleaner02023-07-25T15:25:05ZDUMMY:D1890.9995196residue_name_numbercleaner02023-07-25T15:25:10ZDUMMY:D1900.9994437structure_elementcleaner02023-07-25T14:57:18ZSO:TTD0.99953413residue_name_numbercleaner02023-07-25T15:25:20ZDUMMY:R649bond_interactionMESH:cleaner02023-07-25T15:25:44Zpacks against0.9995499residue_name_numbercleaner02023-07-25T15:25:49ZDUMMY:D1420.9995509residue_name_numbercleaner02023-07-25T15:25:53ZDUMMY:E1530.9995384residue_name_numbercleaner02023-07-25T15:25:59ZDUMMY:S6510.9962274bond_interactioncleaner02023-07-25T15:24:59ZMESH:hydrogen bonds0.99953425residue_name_numbercleaner02023-07-25T15:26:05ZDUMMY:G2360.9995196residue_name_numbercleaner02023-07-25T15:26:11ZDUMMY:W2380.99586034bond_interactioncleaner02023-07-25T15:24:59ZMESH:hydrogen bonds0.99951375residue_name_numbercleaner02023-07-25T15:26:17ZDUMMY:K6500.99952185residue_name_numbercleaner02023-07-25T15:26:21ZDUMMY:A6520.99952614residue_name_numbercleaner02023-07-25T15:26:27ZDUMMY:G6530.9995352residue_name_numbercleaner02023-07-25T15:26:31ZDUMMY:G6540.9989109structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.9995413residue_name_numbercleaner02023-07-25T15:26:36ZDUMMY:N2280.9995378residue_name_numbercleaner02023-07-25T15:26:06ZDUMMY:G2360.999521residue_name_numbercleaner02023-07-25T15:26:12ZDUMMY:W2380.99935097structure_elementcleaner02023-07-25T14:57:18ZSO:TTDRESULTSparagraph9675In support of above structural analyses, mutation D142A/E153A of the TTD abolished its interaction with the Spacer (Fig. 3e). Mutations K648D and S651D of the Spacer decreased their binding affinities to the TTD, and mutation R649A of the Spacer showed more significant decrease (∼13-fold) in the binding affinity (Fig. 3f). As negative control, mutations S639D and S666D of the Spacer showed little effect on the interaction. Interestingly, phosphorylation at residue S651 of UHRF1 was observed in previous mass-spectrometry analyses. Compared with the unmodified peptide of Spacer642–664, a phosphorylation at S651 markedly decreased the binding affinity to the TTD (Supplementary Fig. 2b), suggesting that the phosphorylation may regulate the intramolecular interaction within UHRF1.0.99829507experimental_methodcleaner02023-07-25T16:39:46ZMESH:structural analyses0.99609274experimental_methodcleaner02023-07-25T16:39:49ZMESH:mutation0.99899954mutantcleaner02023-07-25T15:26:52ZMESH:D142A0.9990146mutantcleaner02023-07-25T15:26:57ZMESH:E153A0.9994748structure_elementcleaner02023-07-25T14:57:18ZSO:TTD0.9990018structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.99218035experimental_methodcleaner02023-07-25T16:39:52ZMESH:Mutations0.99906534mutantcleaner02023-07-25T15:27:03ZMESH:K648D0.9990694mutantcleaner02023-07-25T15:27:08ZMESH:S651D0.9989484structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.9987575evidencecleaner02023-07-25T16:44:57ZDUMMY:binding affinities0.9994055structure_elementcleaner02023-07-25T14:57:18ZSO:TTD0.99432313experimental_methodcleaner02023-07-25T16:39:55ZMESH:mutation0.99906355mutantcleaner02023-07-25T15:27:14ZMESH:R649A0.9989303structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.9987483evidencecleaner02023-07-25T15:10:17ZDUMMY:binding affinity0.98485273experimental_methodcleaner02023-07-25T16:39:57ZMESH:mutations0.9991134mutantcleaner02023-07-25T15:27:20ZMESH:S639D0.99907804mutantcleaner02023-07-25T15:27:26ZMESH:S666D0.9987979structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.99615014ptmcleaner02023-07-25T15:27:38ZMESH:phosphorylation0.9994925residue_name_numbercleaner02023-07-25T15:25:59ZDUMMY:S6510.9993629proteincleaner02023-07-25T14:58:02ZPR:UHRF10.9987564experimental_methodcleaner02023-07-25T16:39:59ZMESH:mass-spectrometry0.99917954protein_statecleaner02023-07-25T15:27:58ZDUMMY:unmodified0.96082616mutantcleaner02023-07-25T16:38:03ZMESH:Spacer642–6640.99539644ptmcleaner02023-07-25T15:27:39ZMESH:phosphorylation0.99948895residue_name_numbercleaner02023-07-25T15:26:00ZDUMMY:S6510.9987745evidencecleaner02023-07-25T15:10:17ZDUMMY:binding affinity0.9994197structure_elementcleaner02023-07-25T14:57:18ZSO:TTD0.9951279ptmcleaner02023-07-25T15:27:39ZMESH:phosphorylation0.9993599proteincleaner02023-07-25T14:58:02ZPR:UHRF1RESULTStitle_210466The spacer binds to the TTD by competing with the linker0.99911445structure_elementcleaner02023-07-25T14:57:04ZSO:spacerprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.99956805structure_elementcleaner02023-07-25T14:57:18ZSO:TTD0.999511structure_elementcleaner02023-07-25T15:34:28ZSO:linkerRESULTSparagraph10523Previous studies indicate that the TTD binds to a linker region connecting the TTD and PHD (residues 286–306, designated Linker, Fig. 1a), and TTD–Linker interaction is essential for H3K9me3 recognition by TTD–PHD. Comparison of TTD–Spacer and TTD–PHD–H3K9me3 (PDB: 4GY5) structures indicates that the Spacer and the Linker bind to the TTD in a similar manner in the two complexes (Fig. 3b). In TTD–PHD–H3K9me3 structure, residues R295, R296 and S298 of the Linker adopt almost identical conformation to residues K648, R649 and S651 of the Spacer in TTD–Spacer structure, respectively. Similar intramolecular contacts (TTD–Linker and TTD–Spacer) were observed in the two structures (Fig. 3b,d and Supplementary Fig. 4a). Thus, the Spacer may disrupt the TTD–Linker interaction and inhibits the recognition of H3K9me3 by TTD–PHD.0.99946636structure_elementcleaner02023-07-25T14:57:18ZSO:TTDprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.96558005structure_elementcleaner02023-07-25T16:34:32ZSO:linker region0.99939203structure_elementcleaner02023-07-25T14:57:18ZSO:TTD0.9994456structure_elementcleaner02023-07-25T14:57:49ZSO:PHD0.9974093residue_rangecleaner02023-07-25T16:38:11ZDUMMY:286–3060.9985744structure_elementcleaner02023-07-25T15:34:28ZSO:Linkerstructure_elementSO:cleaner02023-07-25T15:38:48ZTTD–Linkerprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:42:09ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:42:19ZK9me3structure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHD0.68116075experimental_methodcleaner02023-07-25T16:40:06ZMESH:Comparison0.9833022structure_elementcleaner02023-07-25T15:17:21ZSO:TTD–Spacer0.99882823complex_assemblycleaner02023-07-25T15:22:29ZGO:TTD–PHD–H3K9me30.9967913evidencecleaner02023-07-25T16:45:05ZDUMMY:structures0.9982766structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.99900836structure_elementcleaner02023-07-25T15:34:28ZSO:Linker0.999508structure_elementcleaner02023-07-25T14:57:19ZSO:TTD0.9989344complex_assemblycleaner02023-07-25T15:22:29ZGO:TTD–PHD–H3K9me30.9972197evidencecleaner02023-07-25T16:45:08ZDUMMY:structure0.99949956residue_name_numbercleaner02023-07-25T15:29:27ZDUMMY:R2950.99949014residue_name_numbercleaner02023-07-25T15:29:32ZDUMMY:R2960.9995214residue_name_numbercleaner02023-07-25T15:29:38ZDUMMY:S2980.998787structure_elementcleaner02023-07-25T15:34:29ZSO:Linker0.9995097residue_name_numbercleaner02023-07-25T15:25:16ZDUMMY:K6480.9995159residue_name_numbercleaner02023-07-25T15:25:21ZDUMMY:R6490.9995147residue_name_numbercleaner02023-07-25T15:26:00ZDUMMY:S6510.99832743structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.98054665structure_elementcleaner02023-07-25T15:17:21ZSO:TTD–Spacer0.9964238evidencecleaner02023-07-25T16:45:10ZDUMMY:structurestructure_elementSO:cleaner02023-07-25T15:38:48ZTTD–Linkerstructure_elementSO:cleaner02023-07-25T15:17:21ZTTD–Spacer0.998024evidencecleaner02023-07-25T16:45:12ZDUMMY:structures0.9961163structure_elementcleaner02023-07-25T14:57:04ZSO:Spacerstructure_elementSO:cleaner02023-07-25T15:38:48ZTTD–Linkerprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:42:32ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:42:41ZK9me3structure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHDRESULTSparagraph11378To test this hypothesis, we first investigated the potential competition between the Linker and the Spacer for their interaction with the TTD. The ITC experiment shows that the Linker peptide (289–306) bound to the TTD with a binding affinity of 24.04 μM (Supplementary Fig. 4b), ∼15-fold lower than that of the Spacer peptide (KD=1.59 μM, Fig. 3e). The competitive ITC experiments show that TTD–Spacer binding affinity decreased by a factor of two in the presence of the Linker, whereas TTD–Linker interaction was abolished in the presence of the Spacer (Supplementary Fig. 4c). Compared with TTD–Spacer interaction (KD=1.48 μM), TTD–PHD decreased the binding affinity to the Spacer (KD=10.68 μM), whereas mutation R295D/R296D (within the Linker and important for TTD–Linker interaction) of TTD–PHD showed minor decrease in the binding affinity (KD=2.69 μM; Fig. 3g), indicating a competition between the Spacer and the Linker on the same binding site of the TTD. Notably, although the Linker (in the context of TTD-PHD) impairs the TTD–Spacer interaction to some extent, the isolated Spacer could still bind to TTD–PHD with moderate binding affinity (KD=10.68 μM), supporting the existence of the intramolecular interaction within UHRF1.0.99596643structure_elementcleaner02023-07-25T15:34:29ZSO:Linker0.9945722structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.99871874structure_elementcleaner02023-07-25T14:57:19ZSO:TTD0.99860686experimental_methodcleaner02023-07-25T16:40:11ZMESH:ITC0.71808165structure_elementcleaner02023-07-25T15:34:29ZSO:Linker0.99674845residue_rangecleaner02023-07-25T16:38:16ZDUMMY:289–3060.9987063protein_statecleaner02023-07-25T15:10:32ZDUMMY:bound to0.9986609structure_elementcleaner02023-07-25T14:57:19ZSO:TTD0.99879277evidencecleaner02023-07-25T15:10:17ZDUMMY:binding affinity0.64477235structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.9950499evidencecleaner02023-07-25T15:10:11ZDUMMY:KD0.9985807experimental_methodcleaner02023-07-25T16:40:14ZMESH:competitive ITCevidenceDUMMY:cleaner02023-07-25T15:30:27ZTTD–Spacer binding affinity0.9988144protein_statecleaner02023-07-25T15:10:25ZDUMMY:presence of0.9507958structure_elementcleaner02023-07-25T15:34:29ZSO:Linkerstructure_elementSO:cleaner02023-07-25T15:38:48ZTTD–Linker0.9985734protein_statecleaner02023-07-25T15:10:25ZDUMMY:presence of0.941291structure_elementcleaner02023-07-25T14:57:04ZSO:Spacerstructure_elementSO:cleaner02023-07-25T15:17:21ZTTD–Spacer0.9873067evidencecleaner02023-07-25T15:10:11ZDUMMY:KD0.9745458structure_elementcleaner02023-07-25T15:11:21ZSO:TTD–PHD0.9986774evidencecleaner02023-07-25T15:10:17ZDUMMY:binding affinity0.9826287structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.9945904evidencecleaner02023-07-25T15:10:11ZDUMMY:KD0.9967871experimental_methodcleaner02023-07-25T16:40:17ZMESH:mutation0.9991032mutantcleaner02023-07-25T15:37:56ZMESH:R295D0.9991129mutantcleaner02023-07-25T15:38:01ZMESH:R296D0.99915695structure_elementcleaner02023-07-25T15:34:29ZSO:Linkerstructure_elementSO:cleaner02023-07-25T15:38:48ZTTD–Linker0.98462725structure_elementcleaner02023-07-25T15:11:21ZSO:TTD–PHD0.99872434evidencecleaner02023-07-25T15:10:17ZDUMMY:binding affinity0.9955218evidencecleaner02023-07-25T15:10:11ZDUMMY:KD0.95129967structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.99688077structure_elementcleaner02023-07-25T15:34:29ZSO:Linker0.989062sitecleaner02023-07-25T16:51:36ZSO:binding site0.9976774structure_elementcleaner02023-07-25T14:57:19ZSO:TTD0.9987902structure_elementcleaner02023-07-25T15:34:29ZSO:Linker0.9926465structure_elementcleaner02023-07-25T15:31:29ZSO:TTD-PHDstructure_elementSO:cleaner02023-07-25T15:17:21ZTTD–Spacer0.9983601structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.98772246structure_elementcleaner02023-07-25T15:11:21ZSO:TTD–PHD0.9986695evidencecleaner02023-07-25T15:10:17ZDUMMY:binding affinity0.9967951evidencecleaner02023-07-25T15:10:11ZDUMMY:KD0.99931896proteincleaner02023-07-25T14:58:02ZPR:UHRF1RESULTSparagraph12656To test whether TTD–Spacer association exists in the context of full-length UHRF1, we used various truncations of UHRF1 in the GST pull-down assay. As indicated in Fig. 3h, full-length UHRF1 and UHRF1ΔSRA showed no interaction with GST-tagged TTD, Linker or Spacer, suggesting that TTD–Spacer interaction in-cis within full-length UHRF1 or UHRF1ΔSRA prohibits TTD–Spacer complex formation in-trans. In contrast, UHRF1ΔTTD bound to GST-TTD, and UHRF1Δ627–674 bound to GST-Spacer, indicating that lack of TTD–Spacer interaction in-cis, TTD–Spacer complex could form in-trans, supporting that the TTD binds to the Spacer in the context of full-length UHRF1. Moreover, GST-Linker showed very weak if not undetectable interaction with wild-type or deletions of UHRF1, suggesting that TTD–Linker interaction is much weaker than that of TTD–Spacer. Taken together, UHRF1 adopts a closed conformation, in which the Spacer binds to the TTD through competing with the Linker, and therefore inhibits H3K9me3 recognition by UHRF1.structure_elementSO:cleaner02023-07-25T15:17:21ZTTD–Spacer0.99904275protein_statecleaner02023-07-25T15:09:00ZDUMMY:full-length0.99914956proteincleaner02023-07-25T14:58:02ZPR:UHRF10.56910294experimental_methodcleaner02023-07-25T16:40:20ZMESH:truncations0.9991579proteincleaner02023-07-25T14:58:02ZPR:UHRF10.9987602experimental_methodcleaner02023-07-25T16:40:24ZMESH:GST pull-down assay0.99908614protein_statecleaner02023-07-25T15:09:00ZDUMMY:full-length0.9987546proteincleaner02023-07-25T14:58:02ZPR:UHRF10.9988024mutantcleaner02023-07-25T15:32:20ZMESH:UHRF1ΔSRA0.9920395protein_statecleaner02023-07-25T16:48:12ZDUMMY:GST-tagged0.99950266structure_elementcleaner02023-07-25T14:57:19ZSO:TTD0.99916816structure_elementcleaner02023-07-25T15:34:28ZSO:Linker0.9989662structure_elementcleaner02023-07-25T14:57:04ZSO:Spacerstructure_elementSO:cleaner02023-07-25T15:17:21ZTTD–Spacerprotein_stateDUMMY:cleaner02023-07-25T16:48:55Zin-cis0.9990645protein_statecleaner02023-07-25T15:09:00ZDUMMY:full-length0.9991033proteincleaner02023-07-25T14:58:02ZPR:UHRF10.9989047mutantcleaner02023-07-25T15:32:21ZMESH:UHRF1ΔSRAstructure_elementSO:cleaner02023-07-25T15:17:21ZTTD–Spacerprotein_stateDUMMY:cleaner02023-07-25T16:48:36Zin-trans0.99888223mutantcleaner02023-07-25T15:32:49ZMESH:UHRF1ΔTTD0.9990232protein_statecleaner02023-07-25T15:10:33ZDUMMY:bound to0.6337208experimental_methodcleaner02023-07-25T15:33:05ZMESH:GST0.9788821structure_elementcleaner02023-07-25T14:57:19ZSO:TTDmutantMESH:cleaner02023-07-25T15:33:25ZUHRF1Δ627–6740.99905694protein_statecleaner02023-07-25T15:10:33ZDUMMY:bound to0.72377217experimental_methodcleaner02023-07-25T15:33:34ZMESH:GST0.76302904structure_elementcleaner02023-07-25T14:57:04ZSO:Spacerstructure_elementSO:cleaner02023-07-25T15:17:21ZTTD–Spacerprotein_stateDUMMY:cleaner02023-07-25T16:48:54Zin-cis0.87800574structure_elementcleaner02023-07-25T15:17:21ZSO:TTD–Spacerprotein_stateDUMMY:cleaner02023-07-25T16:48:37Zin-trans0.99933016structure_elementcleaner02023-07-25T14:57:19ZSO:TTDprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.99834144structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.9991protein_statecleaner02023-07-25T15:09:00ZDUMMY:full-length0.9991836proteincleaner02023-07-25T14:58:02ZPR:UHRF10.6764868experimental_methodcleaner02023-07-25T15:33:34ZMESH:GST0.6613762structure_elementcleaner02023-07-25T15:34:29ZSO:Linker0.9990788protein_statecleaner02023-07-25T15:34:10ZDUMMY:wild-type0.9992009proteincleaner02023-07-25T14:58:02ZPR:UHRF1structure_elementSO:cleaner02023-07-25T15:38:48ZTTD–Linkerstructure_elementSO:cleaner02023-07-25T15:17:21ZTTD–Spacer0.99930525proteincleaner02023-07-25T14:58:02ZPR:UHRF10.9992095protein_statecleaner02023-07-25T14:59:20ZDUMMY:closed0.9988832structure_elementcleaner02023-07-25T14:57:04ZSO:Spacerprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.99944085structure_elementcleaner02023-07-25T14:57:19ZSO:TTD0.9992781structure_elementcleaner02023-07-25T15:34:29ZSO:Linkerprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T13:01:16ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T13:01:24ZK9me30.99924016proteincleaner02023-07-25T14:58:02ZPR:UHRF1RESULTStitle_213695The spacer inhibits H3K9me3 recognition by the isolated TTD0.9306218structure_elementcleaner02023-07-25T14:57:04ZSO:spacerprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:43:00ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:43:10ZK9me30.99938285structure_elementcleaner02023-07-25T14:57:19ZSO:TTDRESULTSparagraph13755Our previous study indicates that H3K9me3 binds to the TTD in different manner in TTD–PHD–H3K9me3 (ref.) and TTD-H3K9me3 (PDB: 2L3R) structures. Because the TTD is always associated with the PHD, whether the pattern of TTD–H3K9me3 interaction exists in vivo remains unknown. Nevertheless, comparison of TTD–H3K9me3 and TTD–Spacer structures indicates that H3K9me3 and the Spacer overlap on the surface of the TTD (Supplementary Fig. 4d), suggesting that the Spacer might block the H3K9me3 recognition by the isolated TTD. As shown in Supplementary Fig. 4e, the Spacer inhibited TTD–H3K9me3 interaction, whereas its TTD-binding defective mutants of the Spacer or the SRA (a negative control) markedly decreased the inhibition.protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:43:23ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:43:38ZK9me3protein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.9995209structure_elementcleaner02023-07-25T14:57:19ZSO:TTD0.99807155complex_assemblycleaner02023-07-25T15:22:29ZGO:TTD–PHD–H3K9me30.99896175complex_assemblycleaner02023-07-25T15:35:15ZGO:TTD-H3K9me30.9970138evidencecleaner02023-07-25T16:45:20ZDUMMY:structures0.99952006structure_elementcleaner02023-07-25T14:57:19ZSO:TTD0.99954337structure_elementcleaner02023-07-25T14:57:49ZSO:PHDcomplex_assemblyGO:cleaner02023-07-25T15:35:48ZTTD–H3K9me30.66193366experimental_methodcleaner02023-07-25T16:40:31ZMESH:comparison0.9964395complex_assemblycleaner02023-07-25T15:35:47ZGO:TTD–H3K9me30.998569structure_elementcleaner02023-07-25T15:17:21ZSO:TTD–Spacer0.99715745evidencecleaner02023-07-25T16:45:22ZDUMMY:structuresprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:43:52ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:44:00ZK9me30.9979061structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.99949706structure_elementcleaner02023-07-25T14:57:19ZSO:TTD0.99820197structure_elementcleaner02023-07-25T14:57:04ZSO:Spacerprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:44:19ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:44:28ZK9me30.9992218structure_elementcleaner02023-07-25T14:57:19ZSO:TTD0.9980301structure_elementcleaner02023-07-25T14:57:04ZSO:Spacercomplex_assemblyGO:cleaner02023-07-25T15:35:48ZTTD–H3K9me30.99777913protein_statecleaner02023-07-25T15:36:16ZDUMMY:TTD-binding defective0.92626643protein_statecleaner02023-07-25T16:00:01ZDUMMY:mutants0.9984737structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.554281structure_elementcleaner02023-07-25T14:57:34ZSO:SRARESULTSparagraph14493We next tested whether such inhibition also occurs in the context of full-length UHRF1. Compared with full-length UHRF1, UHRF1Δ627–674 enhanced H3K9me3-binding affinity by a factor of four (Supplementary Fig. 4f). The restoration of H3K9me3-binding affinity is not dramatic because the PHD still binds to histone H3 in both proteins. To exclude this effect, we performed the assay using UHRF1D334A, which abolishes H3R2-binding affinity of the PHD. UHRF1D334A showed undetectable H3K9me3-binding affinity, whereas UHRF1D334A&Δ627–674 dramatically restored its H3K9me3-binding affinity (KD=8.69 μM; Supplementary Fig. 4f), indicating that H3K9me3 recognition by the TTD is blocked by the Spacer through competitive interaction with the TTD. Moreover, the R295D/R296D mutant of full-length UHRF1 showed decreased binding affinity to H3K9me3 (eightfold lower than wild type), suggesting that mutation of R295D/R296D favours TTD–Spacer interaction and therefore promotes UHRF1 to exhibit a more stable closed conformation (Supplementary Fig. 4g). Taken together, the Spacer binds to the TTD and inhibits H3K9me3 recognition by UHRF1 through (i) disrupting TTD–Linker interaction, which is essential for H3K9me3 recognition by TTD–PHD, (ii) prohibiting H3K9me3 binding to the isolated TTD.0.99906915protein_statecleaner02023-07-25T15:09:00ZDUMMY:full-length0.99927145proteincleaner02023-07-25T14:58:02ZPR:UHRF10.9990279protein_statecleaner02023-07-25T15:09:00ZDUMMY:full-length0.99917525proteincleaner02023-07-25T14:58:02ZPR:UHRF10.8828556mutantcleaner02023-07-25T15:33:26ZMESH:UHRF1Δ627–6740.9981193evidencecleaner02023-07-25T15:36:48ZDUMMY:H3K9me3-binding affinity0.9981266evidencecleaner02023-07-25T15:36:49ZDUMMY:H3K9me3-binding affinity0.99945194structure_elementcleaner02023-07-25T14:57:50ZSO:PHDprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.95636517protein_typecleaner02023-07-25T15:05:08ZMESH:histone0.80781966protein_typecleaner02023-07-25T15:07:50ZMESH:H30.9991373mutantcleaner02023-07-25T15:37:36ZMESH:UHRF1D334A0.6050428protein_statecleaner02023-07-25T16:49:05ZDUMMY:abolishes0.9379358evidencecleaner02023-07-25T15:37:28ZDUMMY:H3R2-binding affinity0.9994092structure_elementcleaner02023-07-25T14:57:50ZSO:PHD0.9990056mutantcleaner02023-07-25T15:37:37ZMESH:UHRF1D334A0.9981046evidencecleaner02023-07-25T15:36:49ZDUMMY:H3K9me3-binding affinity0.99837047mutantcleaner02023-07-25T15:37:37ZMESH:UHRF1D334A0.95115536mutantcleaner02023-07-25T16:35:23ZMESH:Δ627–6740.9983492evidencecleaner02023-07-25T15:36:49ZDUMMY:H3K9me3-binding affinity0.99851674evidencecleaner02023-07-25T15:10:11ZDUMMY:KDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:44:46ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:44:54ZK9me30.999483structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.9982369structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.9995005structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.99909306mutantcleaner02023-07-25T15:37:56ZMESH:R295D0.99889565mutantcleaner02023-07-25T15:38:01ZMESH:R296D0.9992405protein_statecleaner02023-07-25T16:00:50ZDUMMY:mutant0.99901754protein_statecleaner02023-07-25T15:09:00ZDUMMY:full-length0.9991984proteincleaner02023-07-25T14:58:02ZPR:UHRF10.9981595evidencecleaner02023-07-25T15:10:17ZDUMMY:binding affinityprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:45:16ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:45:10ZK9me30.99894714protein_statecleaner02023-07-25T15:59:54ZDUMMY:wild type0.9975069experimental_methodcleaner02023-07-25T16:40:37ZMESH:mutation0.9991567mutantcleaner02023-07-25T15:37:56ZMESH:R295D0.9989969mutantcleaner02023-07-25T15:38:01ZMESH:R296Dstructure_elementSO:cleaner02023-07-25T15:17:21ZTTD–Spacer0.99926156proteincleaner02023-07-25T14:58:02ZPR:UHRF10.99921596protein_statecleaner02023-07-25T14:59:20ZDUMMY:closed0.9969368structure_elementcleaner02023-07-25T14:57:04ZSO:Spacerprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.9995086structure_elementcleaner02023-07-25T14:57:20ZSO:TTDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:45:35ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:45:44ZK9me30.99934036proteincleaner02023-07-25T14:58:02ZPR:UHRF1structure_elementSO:cleaner02023-07-25T15:38:48ZTTD–Linkerprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:45:56ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:46:05ZK9me3structure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:46:17ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:46:26ZK9me30.9995049structure_elementcleaner02023-07-25T14:57:20ZSO:TTDRESULTStitle_215793TTD–PHD–H3K9me3 complex inhibits TTD–spacer interaction0.99889505complex_assemblycleaner02023-07-25T15:22:29ZGO:TTD–PHD–H3K9me3structure_elementSO:cleaner02023-07-25T15:17:21ZTTD–spacerRESULTSparagraph15855Interestingly, pre-incubation of H3K9me3 peptide completely blocked the interaction between the Spacer and the TTD alone or TTD–PHD (Supplementary Fig. 4h), whereas the presence of the Spacer partially impaired the interaction between TTD–PHD and H3K9me3 (Fig. 2c). The results are also consistent with the previous observation that the interaction between TTD–PHD and the Spacer is much weaker (KD=10.68 μM, Fig. 3g) than that between TTD–PHD and H3K9me3 (KD=0.15 μM, Fig. 1d). These results suggest that once TTD–PHD binds to H3K9me3, UHRF1 will be locked by H3K9me3 and the Spacer is unlikely to fold back for the intramolecular interaction.0.9957268experimental_methodcleaner02023-07-25T16:40:41ZMESH:pre-incubationprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:46:39ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:46:48ZK9me30.9989543structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.99953127structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.9454766protein_statecleaner02023-07-25T16:49:08ZDUMMY:alone0.75461227structure_elementcleaner02023-07-25T15:11:21ZSO:TTD–PHD0.77001154protein_statecleaner02023-07-25T15:10:25ZDUMMY:presence of0.99875605structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.9114364structure_elementcleaner02023-07-25T15:11:21ZSO:TTD–PHDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:47:02ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:47:17ZK9me30.9728093structure_elementcleaner02023-07-25T15:11:21ZSO:TTD–PHD0.9988933structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.9838965evidencecleaner02023-07-25T15:10:11ZDUMMY:KD0.80197483structure_elementcleaner02023-07-25T15:11:21ZSO:TTD–PHDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:47:31ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:47:41ZK9me30.98408234evidencecleaner02023-07-25T15:10:11ZDUMMY:KD0.98543686structure_elementcleaner02023-07-25T15:11:21ZSO:TTD–PHDprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds toprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:48:16ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:48:25ZK9me30.99848336proteincleaner02023-07-25T14:58:02ZPR:UHRF1protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:47:55ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:48:04ZK9me30.9985475structure_elementcleaner02023-07-25T14:57:04ZSO:SpacerRESULTStitle_216516Hm-DNA disrupts intramolecular interaction within UHRF10.99885005chemicalcleaner02023-07-25T14:58:25ZCHEBI:Hm-DNA0.9987213proteincleaner02023-07-25T14:58:02ZPR:UHRF1RESULTSparagraph16572To investigate whether hm-DNA could open the closed conformation of UHRF1, we first measured the intramolecular interaction using UHRF1 truncations in the presence or absence of hm-DNA. The GST pull-down assays show that the PHD bound to the SRA and such interaction was impaired by the addition of hm-DNA (Fig. 4a). H3 peptide pull-down assays show that hm-DNA only enhanced the H3K9me0-binding affinities of UHRF1 truncations containing PHD-SRA, such as PHD-SRA, TTD-PHD-SRA, TTD-PHD-SRA-Spacer, UHRF1ΔTTD and UHRF1ΔSpacer (Fig. 4b). The result indicates that hm-DNA disrupts PHD–SRA interaction and facilitates H3K9me0-binding affinity of the PHD in a manner independent on the TTD or the Spacer.0.9984625chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.99766964protein_statecleaner02023-07-25T16:49:12ZDUMMY:open0.9992649protein_statecleaner02023-07-25T14:59:20ZDUMMY:closed0.9991647proteincleaner02023-07-25T14:58:02ZPR:UHRF10.9961184proteincleaner02023-07-25T14:58:02ZPR:UHRF10.28094894experimental_methodcleaner02023-07-25T16:40:48ZMESH:truncations0.95577383protein_statecleaner02023-07-25T16:49:16ZDUMMY:presence0.99804974protein_statecleaner02023-07-25T15:50:14ZDUMMY:absence of0.99866885chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.9987156experimental_methodcleaner02023-07-25T15:44:48ZMESH:GST pull-down assays0.9994842structure_elementcleaner02023-07-25T14:57:50ZSO:PHD0.9988121protein_statecleaner02023-07-25T15:10:33ZDUMMY:bound to0.99894243structure_elementcleaner02023-07-25T14:57:34ZSO:SRA0.99869126chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.99809307experimental_methodcleaner02023-07-25T15:44:56ZMESH:H3 peptide pull-down assays0.998354chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.99781346evidencecleaner02023-07-25T15:45:08ZDUMMY:H3K9me0-binding affinities0.9794041proteincleaner02023-07-25T14:58:02ZPR:UHRF10.385163experimental_methodcleaner02023-07-25T16:35:37ZMESH:truncationsstructure_elementSO:cleaner02023-07-25T15:16:51ZPHD-SRAstructure_elementSO:cleaner02023-07-25T15:16:51ZPHD-SRAstructure_elementSO:cleaner02023-07-25T15:46:46ZTTD-PHD-SRAstructure_elementSO:cleaner02023-07-25T15:47:05ZTTD-PHD-SRA-Spacer0.99860424mutantcleaner02023-07-25T15:32:50ZMESH:UHRF1ΔTTD0.9987993mutantcleaner02023-07-25T15:47:15ZMESH:UHRF1ΔSpacer0.99783105chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNAstructure_elementSO:cleaner02023-07-25T15:47:39ZPHD–SRA0.9978884evidencecleaner02023-07-25T15:47:57ZDUMMY:H3K9me0-binding affinity0.9994618structure_elementcleaner02023-07-25T14:57:50ZSO:PHD0.99946874structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.99923813structure_elementcleaner02023-07-25T14:57:04ZSO:SpacerRESULTSparagraph17280Moreover, the TTD or TTD–PHD bound to SRA–Spacer and the interaction was impaired by the addition of hm-DNA (Fig. 4c). The ITC measurements show that the presence of hm-DNA markedly impaired the interaction between the TTD and SRA–Spacer (Supplementary Fig. 5a). However, the TTD–Spacer interaction was not affected by the presence of the hm-DNA, indicating that hm-DNA displaces the Spacer from the TTD in a SRA-dependent manner (Supplementary Fig. 5b).0.99950933structure_elementcleaner02023-07-25T14:57:20ZSO:TTDstructure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHD0.9987109protein_statecleaner02023-07-25T15:10:33ZDUMMY:bound tostructure_elementSO:cleaner02023-07-25T15:48:54ZSRA–Spacer0.99908704chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.9987098experimental_methodcleaner02023-07-25T16:40:53ZMESH:ITC0.9988615protein_statecleaner02023-07-25T15:10:25ZDUMMY:presence of0.99899316chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.99947697structure_elementcleaner02023-07-25T14:57:20ZSO:TTDstructure_elementSO:cleaner02023-07-25T15:48:55ZSRA–Spacerstructure_elementSO:cleaner02023-07-25T15:17:21ZTTD–Spacerprotein_stateDUMMY:cleaner02023-07-25T15:10:25Zpresence of0.99896955chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.9988248chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.99763024structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.9994855structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.8576784structure_elementcleaner02023-07-25T14:57:34ZSO:SRARESULTSparagraph17743To investigate whether hm-DNA disrupts TTD–Spacer interaction in the context of full-length UHRF1, we monitored the conformational changes of UHRF1 using its histone-binding affinity as read-out. UHRF1D334A was used to exclude the effect of H3K9me0 recognition by the PHD. As expected, all D334A-containing mutants showed undetectable interaction with H3K9me0 (Fig. 4d). UHRF1D334A bound to H3K9me3 peptide in the presence of hm-DNA, but showed no interaction in the absence of hm-DNA, which is consistent with the ITC experiments (Supplementary Fig. 4f). In contrast, UHRF1D334A&Δ627–674 strongly bound to H3K9me3 even in the absence of hm-DNA (Fig. 4d), indicating that the deletion of the Spacer releases otherwise blocked TTD–PHD for H3K9me3 recognition. The results further support the conclusion that the Spacer binds to the TTD in the context of full-length UHRF1 and the intramolecular interactions are disrupted by hm-DNA.0.9971008chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNAstructure_elementSO:cleaner02023-07-25T15:17:21ZTTD–Spacer0.9991389protein_statecleaner02023-07-25T15:09:00ZDUMMY:full-length0.99922895proteincleaner02023-07-25T14:58:02ZPR:UHRF1proteinPR:cleaner02023-07-25T15:49:52ZUHRF10.9983196evidencecleaner02023-07-25T15:49:57ZDUMMY:histone-binding affinity0.9989108mutantcleaner02023-07-25T15:37:37ZMESH:UHRF1D334Aprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:48:43ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:48:51ZK9me00.99943894structure_elementcleaner02023-07-25T14:57:50ZSO:PHD0.9989262mutantcleaner02023-07-25T16:35:44ZMESH:D334Aprotein_stateDUMMY:cleaner02023-07-25T16:00:01Zmutantsprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:49:04ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:49:12ZK9me00.99879444mutantcleaner02023-07-25T15:37:37ZMESH:UHRF1D334A0.9990998protein_statecleaner02023-07-25T15:10:33ZDUMMY:bound toprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:49:26ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:49:36ZK9me30.99886876protein_statecleaner02023-07-25T15:10:25ZDUMMY:presence of0.9983613chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.99910927protein_statecleaner02023-07-25T15:50:14ZDUMMY:absence of0.997808chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.99873966experimental_methodcleaner02023-07-25T16:40:58ZMESH:ITC0.99884605mutantcleaner02023-07-25T15:37:37ZMESH:UHRF1D334A0.7490342mutantcleaner02023-07-25T16:35:48ZMESH:Δ627–6740.96783024protein_statecleaner02023-07-25T15:10:33ZDUMMY:bound toprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:49:49ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:50:02ZK9me30.99908817protein_statecleaner02023-07-25T15:50:13ZDUMMY:absence of0.99811006chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.9919852experimental_methodcleaner02023-07-25T16:41:00ZMESH:deletion0.99834204structure_elementcleaner02023-07-25T14:57:04ZSO:Spacerstructure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:50:15ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:50:23ZK9me30.99884856structure_elementcleaner02023-07-25T14:57:04ZSO:Spacerprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.99944717structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.9991366protein_statecleaner02023-07-25T15:09:00ZDUMMY:full-length0.9992418proteincleaner02023-07-25T14:58:02ZPR:UHRF10.9978318chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNARESULTSparagraph18682We next performed similar peptide pull-down assay using two mutants (N228C/G653C and R235C/G654C) generated on UHRF1D334A. Residues N228/R235 from the TTD and G653/G654 from the Spacer were chosen according to the TTD–Spacer complex structure (Supplementary Fig. 5c) so that the replaced Cysteine residues (one from the TTD and one from the Spacer) are physically close enough to each other to form a disulphide bond in the absence of reducing reagent (dithiothreitol, DTT). As shown in Fig. 4d, hm-DNA largely enhanced the H3K9me3-binding affinities of both mutants in the presence of DTT, but not in the absence of DTT, indicating that the disulphide bond formation (in the absence of DTT) disallows hm-DNA to disrupt TTD–Spacer interaction for H3K9me3 recognition. As negative controls, H3K9me3 recognition by UHRF1D334A or UHRF1D334A&Δ627–674 is not affected by DTT.0.99872124experimental_methodcleaner02023-07-25T15:50:32ZMESH:peptide pull-down assayprotein_stateDUMMY:cleaner02023-07-25T16:00:01Zmutants0.9989108mutantcleaner02023-07-25T15:50:37ZMESH:N228C0.9985165mutantcleaner02023-07-25T15:50:42ZMESH:G653C0.99888533mutantcleaner02023-07-25T15:50:47ZMESH:R235C0.9982223mutantcleaner02023-07-25T15:50:53ZMESH:G654C0.99885094mutantcleaner02023-07-25T15:37:37ZMESH:UHRF1D334A0.99957484residue_name_numbercleaner02023-07-25T15:26:37ZDUMMY:N2280.99957687residue_name_numbercleaner02023-07-25T15:51:05ZDUMMY:R2350.99939466structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.99954104residue_name_numbercleaner02023-07-25T15:26:28ZDUMMY:G6530.9995326residue_name_numbercleaner02023-07-25T15:26:32ZDUMMY:G6540.99916935structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.9990664structure_elementcleaner02023-07-25T15:17:21ZSO:TTD–Spacer0.9821614evidencecleaner02023-07-25T16:45:30ZDUMMY:structure0.99367803residue_namecleaner02023-07-25T16:35:17ZSO:Cysteine0.99936646structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.99905914structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.9833516ptmcleaner02023-07-25T15:51:59ZMESH:disulphide bond0.99728125protein_statecleaner02023-07-25T15:50:14ZDUMMY:absence of0.9975418chemicalcleaner02023-07-25T15:51:45ZCHEBI:dithiothreitol0.9974686chemicalcleaner02023-07-25T15:51:50ZCHEBI:DTT0.9978681chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.9978291evidencecleaner02023-07-25T15:51:32ZDUMMY:H3K9me3-binding affinitiesprotein_stateDUMMY:cleaner02023-07-25T16:00:01Zmutants0.99578726protein_statecleaner02023-07-25T15:10:25ZDUMMY:presence of0.99850523chemicalcleaner02023-07-25T15:51:51ZCHEBI:DTT0.99795115protein_statecleaner02023-07-25T15:50:14ZDUMMY:absence of0.9981852chemicalcleaner02023-07-25T15:51:51ZCHEBI:DTT0.97308254ptmcleaner02023-07-25T15:51:59ZMESH:disulphide bond0.9947853protein_statecleaner02023-07-25T15:50:14ZDUMMY:absence of0.99812025chemicalcleaner02023-07-25T15:51:51ZCHEBI:DTT0.9967267chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNAstructure_elementSO:cleaner02023-07-25T15:17:21ZTTD–Spacerprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:50:40ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:50:48ZK9me3protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T13:01:42ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T13:01:50ZK9me30.99848133mutantcleaner02023-07-25T15:37:37ZMESH:UHRF1D334A0.99851066mutantcleaner02023-07-25T15:37:37ZMESH:UHRF1D334A0.9231658mutantcleaner02023-07-25T16:35:52ZMESH:Δ627–6740.99822944chemicalcleaner02023-07-25T15:51:51ZCHEBI:DTTRESULTSparagraph19560The above results collectively demonstrate that (i) full-length UHRF1 adopts a closed form, in which the Spacer binds to the TTD and H3K9me3 recognition is inhibited; (ii) hm-DNA displaces the Spacer from the TTD in the context of full-length UHRF1 and therefore largely enhances its histone H3K9me3-binding activity in a manner independent on the PHD (SRA is required). We have previously demonstrated that hm-DNA also disrupts PHD–SRA interaction and facilitates H3K9me0-binding affinity of the PHD in a manner independent on the TTD or the Spacer. Taken together, hm-DNA disrupts the intramolecular interactions within UHRF1, and therefore facilitates the coordinate recognition of H3K9me3 by TTD–PHD.0.9990444protein_statecleaner02023-07-25T15:09:00ZDUMMY:full-length0.99925977proteincleaner02023-07-25T14:58:02ZPR:UHRF10.9992508protein_statecleaner02023-07-25T14:59:20ZDUMMY:closed0.99818677structure_elementcleaner02023-07-25T14:57:04ZSO:Spacerprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.9995371structure_elementcleaner02023-07-25T14:57:20ZSO:TTDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:51:06ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:51:14ZK9me30.9987197chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.9989048structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.9995277structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.9991288protein_statecleaner02023-07-25T15:09:00ZDUMMY:full-length0.9992617proteincleaner02023-07-25T14:58:02ZPR:UHRF10.73736984protein_typecleaner02023-07-25T15:05:08ZMESH:histoneprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:51:25ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:51:33ZK9me30.9994729structure_elementcleaner02023-07-25T14:57:50ZSO:PHD0.9982949structure_elementcleaner02023-07-25T14:57:35ZSO:SRA0.9982069chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNAstructure_elementSO:cleaner02023-07-25T15:47:40ZPHD–SRA0.9877172evidencecleaner02023-07-25T15:47:58ZDUMMY:H3K9me0-binding affinity0.99947613structure_elementcleaner02023-07-25T14:57:50ZSO:PHD0.99953234structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.99911374structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.9984932chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.99928147proteincleaner02023-07-25T14:58:02ZPR:UHRF1protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:51:45ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:51:54ZK9me3structure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHDRESULTStitle_220269The spacer enhances hm-DNA-binding affinity of the SRA0.82882965structure_elementcleaner02023-07-25T14:57:04ZSO:spacerevidenceDUMMY:cleaner02023-07-25T14:56:50Zhm-DNA-binding affinity0.9169475structure_elementcleaner02023-07-25T14:57:35ZSO:SRARESULTSparagraph20324To investigate how hm-DNA impairs TTD–Spacer interaction, we tested whether the Spacer is involved in hm-DNA recognition by the SRA, which is the only known domain for hm-DNA recognition within UHRF1. In the electrophoretic mobility-shift assay, SRA–Spacer showed higher hm-DNA-binding affinity than the SRA alone (Supplementary Fig. 6a). ITC measurements show that SRA–Spacer bound to hm-DNA with a much higher binding affinity (KD=1.75 μM) than the SRA (KD=25.12 μM), whereas the Spacer alone showed no interaction with hm-DNA (Fig. 5a). In the fluorescence polarization (FP) measurements, SRA–Spacer, full-length UHRF1 and UHRF1ΔTTD showed comparable hm-DNA-binding affinities (Fig. 5b and Supplementary Table 4), suggesting that UHRF1 binds to hm-DNA no matter UHRF1 adopts a closed form or not. In contrast, UHRF1ΔSRA abolished hm-DNA-binding affinity, indicating that the SRA is essential for hm-DNA recognition. Compared with full-length UHRF1, UHRF1Δ627–674 decreased the hm-DNA-binding affinity by a factor of 14 (Fig. 5b), further supporting that the Spacer plays an important role in hm-DNA recognition in the context of full-length UHRF1. In addition, hm-DNA-binding affinities of SRA or SRA–Spacer did not obviously vary upon the change of DNA lengths but did decrease with the increasing salt concentrations (Supplementary Fig. 6b,c and Supplementary Table 5). These results indicate that the Spacer not only binds to the TTD and inhibits H3K9me3 recognition when UHRF1 adopts closed conformation, but also facilitates hm-DNA recognition by the SRA when UHRF1 binds to hm-DNA.chemicalCHEBI:cleaner02023-07-25T14:58:25Zhm-DNAstructure_elementSO:cleaner02023-07-25T15:17:21ZTTD–Spacer0.99807835structure_elementcleaner02023-07-25T14:57:04ZSO:SpacerchemicalCHEBI:cleaner02023-07-25T14:58:25Zhm-DNA0.81657904structure_elementcleaner02023-07-25T14:57:35ZSO:SRAchemicalCHEBI:cleaner02023-07-25T14:58:25Zhm-DNA0.999308proteincleaner02023-07-25T14:58:02ZPR:UHRF10.9981696experimental_methodcleaner02023-07-25T16:41:07ZMESH:electrophoretic mobility-shift assay0.987925structure_elementcleaner02023-07-25T15:48:55ZSO:SRA–Spacer0.99830437evidencecleaner02023-07-25T14:56:50ZDUMMY:hm-DNA-binding affinity0.76229775structure_elementcleaner02023-07-25T14:57:35ZSO:SRA0.98666745protein_statecleaner02023-07-25T16:49:21ZDUMMY:alone0.9979923experimental_methodcleaner02023-07-25T16:41:10ZMESH:ITC0.9918042structure_elementcleaner02023-07-25T15:48:55ZSO:SRA–Spacer0.9989643protein_statecleaner02023-07-25T15:10:33ZDUMMY:bound to0.92392224chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.9986793evidencecleaner02023-07-25T15:10:17ZDUMMY:binding affinity0.9982942evidencecleaner02023-07-25T15:10:11ZDUMMY:KD0.9393552structure_elementcleaner02023-07-25T14:57:35ZSO:SRA0.9979615evidencecleaner02023-07-25T15:10:11ZDUMMY:KD0.94276625structure_elementcleaner02023-07-25T14:57:04ZSO:Spacer0.97806543protein_statecleaner02023-07-25T16:49:23ZDUMMY:alonechemicalCHEBI:cleaner02023-07-25T14:58:25Zhm-DNA0.997146experimental_methodcleaner02023-07-25T16:41:15ZMESH:fluorescence polarization (FP0.9868372structure_elementcleaner02023-07-25T15:48:55ZSO:SRA–Spacer0.99911547protein_statecleaner02023-07-25T15:09:00ZDUMMY:full-length0.99911135proteincleaner02023-07-25T14:58:02ZPR:UHRF10.9990601mutantcleaner02023-07-25T15:32:50ZMESH:UHRF1ΔTTD0.9985642evidencecleaner02023-07-25T15:53:50ZDUMMY:hm-DNA-binding affinities0.9991716proteincleaner02023-07-25T14:58:02ZPR:UHRF1protein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds tochemicalCHEBI:cleaner02023-07-25T14:58:25Zhm-DNA0.9992442proteincleaner02023-07-25T14:58:02ZPR:UHRF10.99919313protein_statecleaner02023-07-25T14:59:20ZDUMMY:closed0.99904126mutantcleaner02023-07-25T15:32:21ZMESH:UHRF1ΔSRA0.99844027evidencecleaner02023-07-25T14:56:50ZDUMMY:hm-DNA-binding affinity0.99079424structure_elementcleaner02023-07-25T14:57:35ZSO:SRAchemicalCHEBI:cleaner02023-07-25T14:58:25Zhm-DNA0.99907523protein_statecleaner02023-07-25T15:09:01ZDUMMY:full-length0.99927646proteincleaner02023-07-25T14:58:02ZPR:UHRF1mutantMESH:cleaner02023-07-25T15:33:26ZUHRF1Δ627–6740.9985644evidencecleaner02023-07-25T14:56:50ZDUMMY:hm-DNA-binding affinity0.9980646structure_elementcleaner02023-07-25T14:57:04ZSO:SpacerchemicalCHEBI:cleaner02023-07-25T14:58:25Zhm-DNA0.99915737protein_statecleaner02023-07-25T15:09:01ZDUMMY:full-length0.999258proteincleaner02023-07-25T14:58:02ZPR:UHRF10.99851686evidencecleaner02023-07-25T15:53:51ZDUMMY:hm-DNA-binding affinities0.86080647structure_elementcleaner02023-07-25T14:57:35ZSO:SRA0.9879043structure_elementcleaner02023-07-25T15:48:55ZSO:SRA–Spacer0.99886537structure_elementcleaner02023-07-25T14:57:05ZSO:Spacerprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.99944586structure_elementcleaner02023-07-25T14:57:20ZSO:TTDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:52:11ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:52:20ZK9me30.999356proteincleaner02023-07-25T14:58:02ZPR:UHRF10.99925965protein_statecleaner02023-07-25T14:59:20ZDUMMY:closedchemicalCHEBI:cleaner02023-07-25T14:58:25Zhm-DNA0.47243175structure_elementcleaner02023-07-25T14:57:35ZSO:SRA0.9991511proteincleaner02023-07-25T14:58:02ZPR:UHRF1protein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds tochemicalCHEBI:cleaner02023-07-25T14:58:25Zhm-DNARESULTSparagraph21936We next mapped the minimal region of the Spacer for the enhancement of hm-DNA-binding affinity. SRA–Spacer-661 (residues 414–661) still maintained strong hm-DNA-binding affinity comparable to that of SRA–Spacer (residues 414–674), whereas SRA–Spacer-652 and SRA–Spacer-642 markedly decreased their hm-DNA-binding affinities (Fig. 5c), indicating that residues 642–661 are important for enhancing hm-DNA-binding affinity of the SRA. This minimal region largely overlaps with the Spacer region (643–655) essential for TTD interaction. We also determined the crystal structure of SRA–Spacer bound to hm-DNA at 3.15 Å resolution (Supplementary Table 6 and Supplementary Fig. 7a). The structure shows that the SRA binds to hm-DNA in a manner similar to that observed in the previously reported SRA-hm-DNA structures. Intriguingly, no electron density was observed for the Spacer. A possible explanation is that the Spacer facilitates SRA–hm-DNA interaction through nonspecific salt bridge contacts because DNA is rich in acidic groups and the Spacer is rich in basic residues (Supplementary Fig. 7b). The nonspecific interaction is consistent with the previous observation that UHRF1 has no DNA sequence selectivity besides hm-CpG dinucleotide.0.9975426structure_elementcleaner02023-07-25T14:57:05ZSO:Spacer0.9973096evidencecleaner02023-07-25T14:56:50ZDUMMY:hm-DNA-binding affinitymutantMESH:cleaner02023-07-25T15:56:40ZSRA–Spacer-6610.9965994residue_rangecleaner02023-07-25T16:38:23ZDUMMY:414–6610.99742836evidencecleaner02023-07-25T14:56:50ZDUMMY:hm-DNA-binding affinity0.8045659structure_elementcleaner02023-07-25T15:48:55ZSO:SRA–Spacer0.99663335residue_rangecleaner02023-07-25T16:38:30ZDUMMY:414–674mutantMESH:cleaner02023-07-25T15:57:14ZSRA–Spacer-652mutantMESH:cleaner02023-07-25T15:57:47ZSRA–Spacer-6420.9978421evidencecleaner02023-07-25T15:53:51ZDUMMY:hm-DNA-binding affinities0.99661374residue_rangecleaner02023-07-25T16:38:34ZDUMMY:642–6610.9960874evidencecleaner02023-07-25T14:56:50ZDUMMY:hm-DNA-binding affinity0.99648416structure_elementcleaner02023-07-25T14:57:35ZSO:SRAstructure_elementSO:cleaner02023-07-25T14:57:05ZSpacer0.99668616residue_rangecleaner02023-07-25T16:38:38ZDUMMY:643–6550.7144268structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.998444evidencecleaner02023-07-25T16:45:37ZDUMMY:crystal structure0.9244888structure_elementcleaner02023-07-25T15:48:55ZSO:SRA–Spacer0.99880683protein_statecleaner02023-07-25T15:10:33ZDUMMY:bound to0.99367905chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.997813evidencecleaner02023-07-25T16:45:39ZDUMMY:structure0.9913696structure_elementcleaner02023-07-25T14:57:35ZSO:SRA0.8255575protein_statecleaner02023-07-25T15:13:04ZDUMMY:binds to0.9931057chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.99895704complex_assemblycleaner02023-07-25T15:58:37ZGO:SRA-hm-DNA0.9978289evidencecleaner02023-07-25T16:45:42ZDUMMY:structures0.9962826evidencecleaner02023-07-25T16:45:45ZDUMMY:electron density0.9974815structure_elementcleaner02023-07-25T14:57:05ZSO:Spacer0.9951716structure_elementcleaner02023-07-25T14:57:05ZSO:Spacercomplex_assemblyGO:cleaner02023-07-25T15:59:10ZSRA–hm-DNA0.9730364bond_interactioncleaner02023-07-25T15:59:17ZMESH:salt bridge0.94352984chemicalcleaner02023-07-25T16:30:22ZCHEBI:DNA0.9973133structure_elementcleaner02023-07-25T14:57:05ZSO:Spacer0.99935216proteincleaner02023-07-25T14:58:02ZPR:UHRF1chemicalCHEBI:cleaner02023-07-25T15:59:31ZDNA0.99895376chemicalcleaner02023-07-25T15:59:37ZCHEBI:hm-CpG dinucleotideRESULTStitle_223199The spacer is important for PCH localization of UHRF10.99830556structure_elementcleaner02023-07-25T14:57:05ZSO:spacer0.9988042proteincleaner02023-07-25T14:58:03ZPR:UHRF1RESULTSparagraph23253To investigate the role of the Spacer in the regulation of UHRF1 function, we transiently overexpressed GFP-tagged wild type or mutants of UHRF1 in NIH3T3 cells to determine their subcellular localization. For the NIH3T3 cells expressing wild-type UHRF1, most cells (∼74.6%) showed a focal pattern of protein that is co-localized with 4,6-diamidino-2-phenylindole (DAPI) foci (Fig. 5d), whereas the rest cells showed a diffuse nuclear staining pattern. The result is consistent with the previous studies that UHRF1 is mainly localized to highly methylated pericentromeric heterochromatin (PCH). In contrast, for the cells expressing UHRF1Δ627–674, a spacer deletion mutant with decreased hm-DNA-binding affinity (Fig. 5b), only ∼22.1% cells showed co-localization with DAPI.0.9981256structure_elementcleaner02023-07-25T14:57:05ZSO:Spacer0.9989207proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9978527experimental_methodcleaner02023-07-25T16:41:23ZMESH:transiently overexpressed0.99685115protein_statecleaner02023-07-25T15:59:46ZDUMMY:GFP-tagged0.999069protein_statecleaner02023-07-25T15:59:53ZDUMMY:wild type0.9961945protein_statecleaner02023-07-25T16:00:00ZDUMMY:mutants0.9990792proteincleaner02023-07-25T14:58:03ZPR:UHRF10.99910134protein_statecleaner02023-07-25T15:34:11ZDUMMY:wild-type0.99903154proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9928932chemicalcleaner02023-07-25T16:00:05ZCHEBI:4,6-diamidino-2-phenylindole0.98459643chemicalcleaner02023-07-25T16:00:09ZCHEBI:DAPI0.999064proteincleaner02023-07-25T14:58:03ZPR:UHRF10.96579576protein_statecleaner02023-07-25T16:49:30ZDUMMY:highly methylated0.9048956mutantcleaner02023-07-25T15:33:26ZMESH:UHRF1Δ627–6740.981091protein_statecleaner02023-07-25T16:00:39ZDUMMY:spacer deletion mutant0.9975831evidencecleaner02023-07-25T14:56:50ZDUMMY:hm-DNA-binding affinity0.80328345chemicalcleaner02023-07-25T16:00:11ZCHEBI:DAPIRESULTSparagraph24035Previous reports have shown that the H3K9me3 recognition of UHRF1 also plays an important role in its heterochromatin localization. For example, UHRF1 mutant (within TTD domain) lacking H3K9me3-binding affinity largely reduces its co-localization with heterochromatin. Because manipulation of endogenous hm-DNA in cells is technically challenging, we used UHRF1ΔSRA (lacks hm-DNA-binding affinity but maintains closed conformation, Figs 3h and 5b) to test whether closed conformation of UHRF1 exists in vivo. In NIH3T3 cells, UHRF1ΔSRA largely decreased chromatin association (Fig. 5d). Only ∼4.8% cells expressing UHRF1ΔSRA showed an intermediate enrichment, but not characteristic focal pattern, at DAPI foci, whereas the majority of the cells showed a diffuse nuclear staining pattern. The results suggest that UHRF1ΔSRA adopts closed conformation so that H3K9me3 recognition by TTD–PHD is blocked by the intramolecular interaction, and support the regulatory role of the Spacer in PCH localization of UHRF1 in vivo.protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:52:39ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:52:50ZK9me30.99911493proteincleaner02023-07-25T14:58:03ZPR:UHRF10.96556795proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9991499protein_statecleaner02023-07-25T16:00:49ZDUMMY:mutant0.9993081structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.9985348protein_statecleaner02023-07-25T16:01:43ZDUMMY:lacking0.9186321evidencecleaner02023-07-25T15:36:49ZDUMMY:H3K9me3-binding affinity0.95978385chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.9990237mutantcleaner02023-07-25T15:32:21ZMESH:UHRF1ΔSRA0.9810548protein_statecleaner02023-07-25T16:01:46ZDUMMY:lacks0.8604536evidencecleaner02023-07-25T14:56:50ZDUMMY:hm-DNA-binding affinityprotein_stateDUMMY:cleaner02023-07-25T14:59:20Zclosedprotein_stateDUMMY:cleaner02023-07-25T14:59:20Zclosed0.99917513proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9986072mutantcleaner02023-07-25T15:32:21ZMESH:UHRF1ΔSRA0.99880767mutantcleaner02023-07-25T15:32:21ZMESH:UHRF1ΔSRAchemicalCHEBI:cleaner02023-07-25T16:00:11ZDAPI0.9989556mutantcleaner02023-07-25T15:32:21ZMESH:UHRF1ΔSRAprotein_stateDUMMY:cleaner02023-07-25T14:59:20Zclosedprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:53:06ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:53:14ZK9me30.80278236structure_elementcleaner02023-07-25T15:11:21ZSO:TTD–PHD0.99870586structure_elementcleaner02023-07-25T14:57:05ZSO:Spacer0.9991685proteincleaner02023-07-25T14:58:03ZPR:UHRF1RESULTStitle_225071The spacer facilitates UHRF1–DNMT1 interaction0.99730814structure_elementcleaner02023-07-25T14:57:05ZSO:spacercomplex_assemblyGO:cleaner02023-07-25T16:03:29ZUHRF1–DNMT1RESULTSparagraph25120Previous studies show that UHRF1 recruits DNMT1 to hm-DNA for maintenance DNA methylation through the interaction between the SRA and RFTSDNMT1 (refs). We confirmed the direct interaction between RFTSDNMT1 and the SRA in a solution with low salt concentration (50 mM NaCl), but observed weak or undetectable interaction in a solution with higher salt concentrations (100 or 150 mM NaCl) (Supplementary Fig. 8a). Compared with the SRA, SRA–Spacer exhibited stronger interaction with RFTSDNMT1. In addition, RFTSDNMT1 bound to SRA–Spacer with a binding affinity of 7.09 μM, but showed no detectable interaction with the SRA (Supplementary Fig. 8b). Interestingly, the addition of hm-DNA abolished the interaction between RFTSDNMT1 and SRA–Spacer, suggesting that hm-DNA also regulates UHRF1–DNMT1 interaction (Supplementary Fig. 8c). These results indicate that the Spacer facilitates the interaction between RFTSDNMT1 and the SRA, and the interaction is impaired by the presence of hm-DNA.0.9991511proteincleaner02023-07-25T14:58:03ZPR:UHRF10.99884087proteincleaner02023-07-25T15:00:38ZPR:DNMT10.99011177chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNAchemicalCHEBI:cleaner02023-07-25T16:02:00ZDNAptmMESH:cleaner02023-07-25T15:05:57Zmethylation0.9738806structure_elementcleaner02023-07-25T14:57:35ZSO:SRA0.99923766proteincleaner02023-07-25T16:02:12ZPR:RFTSDNMT10.9991768proteincleaner02023-07-25T16:02:12ZPR:RFTSDNMT10.9504781structure_elementcleaner02023-07-25T14:57:35ZSO:SRA0.99426115chemicalcleaner02023-07-25T16:02:18ZCHEBI:NaCl0.9910999chemicalcleaner02023-07-25T16:02:19ZCHEBI:NaCl0.990811structure_elementcleaner02023-07-25T14:57:35ZSO:SRA0.9875321structure_elementcleaner02023-07-25T15:48:55ZSO:SRA–Spacer0.9989967proteincleaner02023-07-25T16:02:12ZPR:RFTSDNMT10.9987704proteincleaner02023-07-25T16:02:12ZPR:RFTSDNMT10.99872077protein_statecleaner02023-07-25T15:10:33ZDUMMY:bound to0.8938754structure_elementcleaner02023-07-25T15:48:55ZSO:SRA–Spacer0.99867624evidencecleaner02023-07-25T15:10:18ZDUMMY:binding affinity0.929079structure_elementcleaner02023-07-25T14:57:35ZSO:SRA0.9985009chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNA0.99913883proteincleaner02023-07-25T16:02:12ZPR:RFTSDNMT10.9334615structure_elementcleaner02023-07-25T15:48:55ZSO:SRA–Spacer0.99392587chemicalcleaner02023-07-25T14:58:25ZCHEBI:hm-DNAcomplex_assemblyGO:cleaner02023-07-25T16:03:29ZUHRF1–DNMT10.9990073structure_elementcleaner02023-07-25T14:57:05ZSO:Spacer0.99927133proteincleaner02023-07-25T16:02:12ZPR:RFTSDNMT10.8667852structure_elementcleaner02023-07-25T14:57:36ZSO:SRAprotein_stateDUMMY:cleaner02023-07-25T15:10:25Zpresence of0.9979096chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNARESULTSparagraph26124We next tested whether the UHRF1–DNMT1 interaction is regulated by the conformational change of UHRF1. Because the addition of hm-DNA disrupts the interaction between the SRA–Spacer and RFTSDNMT1, we used various truncations to mimic open and closed forms of UHRF1. In the absence of hm-DNA, only UHRF1ΔTTD bound to RFTSDNMT1, whereas full-length UHRF1, UHRF1ΔSRA and UHRF1Δ627–674 showed undetectable interaction (Fig. 5e). As the deletion of the TTD allows UHRF1 to adopt an open conformation, the results suggest that RFTSDNMT1 binds to SRA–Spacer when UHRF1 adopts an open conformation in the absence of hm-DNA. In support of above observations, the addition of large amount of RFTSDNMT1 impaired the interaction between UHRF1 and hm-DNA (Supplementary Fig. 8d), suggesting an existence of dynamic equilibrium between UHRF1–hm-DNA and UHRF1–DNMT1 complexes.complex_assemblyGO:cleaner02023-07-25T16:03:29ZUHRF1–DNMT10.9991204proteincleaner02023-07-25T14:58:03ZPR:UHRF10.99723744chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.99853516structure_elementcleaner02023-07-25T15:48:55ZSO:SRA–Spacer0.99903846proteincleaner02023-07-25T16:02:12ZPR:RFTSDNMT10.6827299experimental_methodcleaner02023-07-25T16:41:29ZMESH:truncations0.99917966protein_statecleaner02023-07-25T16:49:50ZDUMMY:open0.99918646protein_statecleaner02023-07-25T14:59:20ZDUMMY:closed0.9992004proteincleaner02023-07-25T14:58:03ZPR:UHRF10.998818protein_statecleaner02023-07-25T15:50:14ZDUMMY:absence of0.9959512chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.9990483mutantcleaner02023-07-25T15:32:50ZMESH:UHRF1ΔTTD0.99897456protein_statecleaner02023-07-25T15:10:33ZDUMMY:bound to0.9410995proteincleaner02023-07-25T16:02:12ZPR:RFTSDNMT10.99909276protein_statecleaner02023-07-25T15:09:01ZDUMMY:full-length0.9991623proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9990976mutantcleaner02023-07-25T15:32:21ZMESH:UHRF1ΔSRA0.8580041mutantcleaner02023-07-25T15:33:27ZMESH:UHRF1Δ627–6740.79138756experimental_methodcleaner02023-07-25T16:41:33ZMESH:deletion of0.99919194structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.9992625proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9991698protein_statecleaner02023-07-25T16:49:53ZDUMMY:open0.99846977proteincleaner02023-07-25T16:02:12ZPR:RFTSDNMT1protein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.99843246structure_elementcleaner02023-07-25T15:48:55ZSO:SRA–Spacer0.9992735proteincleaner02023-07-25T14:58:03ZPR:UHRF1protein_stateDUMMY:cleaner02023-07-25T16:50:11Zopen0.9990318protein_statecleaner02023-07-25T15:50:14ZDUMMY:absence of0.9968054chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.5373807experimental_methodcleaner02023-07-25T16:41:39ZMESH:addition0.8506688proteincleaner02023-07-25T16:02:13ZPR:RFTSDNMT10.9990897proteincleaner02023-07-25T14:58:03ZPR:UHRF10.99238986chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNAcomplex_assemblyGO:cleaner02023-07-25T16:04:33ZUHRF1–hm-DNA0.9837528complex_assemblycleaner02023-07-25T16:03:29ZGO:UHRF1–DNMT1DISCUSStitle_127001DiscussionDISCUSSparagraph27012According to the above results, we here proposed a working model for hm-DNA-mediated regulation of UHRF1 conformation (Fig. 5f). In the absence of hm-DNA (A), UHRF1 prefers a closed conformation, in which the Spacer binds to the TTD by competing with the Linker and the SRA binds to the PHD. As a result, the recognition of histone H3K9me3 by the TTD is blocked by the Spacer, and recognition of unmodified histone H3 (H3R2) by the PHD is inhibited by the SRA. The interaction between UHRF1 and DNMT1 is also weak because the Spacer is unable to facilitate the intermolecular interaction. In the presence of hm-DNA (B), UHRF1 prefers an open conformation, in which the SRA binds to the hm-DNA; the Spacer dissociates from the TTD and facilitates the interaction between the SRA and hm-DNA; the Linker binds to the TTD and allows TTD–PHD to recognize histone H3K9me3. When UHRF1 adopts an open conformation and has already bound to H3K9me3 (B), the interaction between H3K9me3 and TTD–PHD further prevents the Spacer from folding back to interact with the TTD, and therefore locks UHRF1 in an open conformation. The association of UHRF1 to the histone may facilitate the ubiquitination of histone tail (mediated by RING domain) for DNMT1 targeting. Moreover, through a mechanism yet to be fully elucidated, DNMT1 targets hm-DNA for maintenance DNA methylation, probably through interaction with the histone ubiquitylation and/or SRA-Spacer. This cartoon summarizes our findings in this study. The P(r) function obtained from small-angle X-ray scattering (SAXS) measurements of TTD–PHD–SRA–Spacer–hm-DNA complex showed a broader distribution than that of the TTD–PHD–SRA–Spacer alone, supporting the proposed model that UHRF1 adopts an open conformation in the presence of hm-DNA (Supplementary Fig. 8e).0.991255chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.9993242proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9991345protein_statecleaner02023-07-25T15:50:14ZDUMMY:absence of0.99493146chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.99937004proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9992467protein_statecleaner02023-07-25T14:59:20ZDUMMY:closed0.99932206structure_elementcleaner02023-07-25T14:57:05ZSO:Spacerprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.999443structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.99939466structure_elementcleaner02023-07-25T15:34:29ZSO:Linker0.9982951structure_elementcleaner02023-07-25T14:57:36ZSO:SRAprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.99953127structure_elementcleaner02023-07-25T14:57:50ZSO:PHD0.756802protein_typecleaner02023-07-25T15:05:08ZMESH:histoneprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:53:34ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:53:42ZK9me30.9994456structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.9991673structure_elementcleaner02023-07-25T14:57:05ZSO:Spacer0.99908257protein_statecleaner02023-07-25T15:27:59ZDUMMY:unmodified0.9225563protein_typecleaner02023-07-25T15:05:08ZMESH:histone0.8794957protein_typecleaner02023-07-25T15:07:50ZMESH:H30.7818248sitecleaner02023-07-25T15:03:31ZSO:H3R20.99932885structure_elementcleaner02023-07-25T14:57:50ZSO:PHD0.998609structure_elementcleaner02023-07-25T14:57:36ZSO:SRA0.99932027proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9992797proteincleaner02023-07-25T15:00:38ZPR:DNMT10.998733structure_elementcleaner02023-07-25T14:57:05ZSO:Spacer0.99915636protein_statecleaner02023-07-25T15:10:25ZDUMMY:presence of0.9977233chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.9993381proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9992636protein_statecleaner02023-07-25T16:50:15ZDUMMY:open0.9976272structure_elementcleaner02023-07-25T14:57:36ZSO:SRAprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.9880094chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.99935025structure_elementcleaner02023-07-25T14:57:05ZSO:Spacer0.9994367structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.98727417structure_elementcleaner02023-07-25T14:57:36ZSO:SRA0.9894224chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.99928206structure_elementcleaner02023-07-25T15:34:29ZSO:Linkerprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.9994356structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.9430154structure_elementcleaner02023-07-25T15:11:21ZSO:TTD–PHD0.7563194protein_typecleaner02023-07-25T15:05:08ZMESH:histoneprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:53:54ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:54:05ZK9me30.9993493proteincleaner02023-07-25T14:58:03ZPR:UHRF1protein_stateDUMMY:cleaner02023-07-25T16:50:34Zopen0.9987874protein_statecleaner02023-07-25T15:10:33ZDUMMY:bound toprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:54:18ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:54:27ZK9me3protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:54:52ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:54:44ZK9me30.9569623structure_elementcleaner02023-07-25T15:11:21ZSO:TTD–PHD0.9993548structure_elementcleaner02023-07-25T14:57:05ZSO:Spacer0.9994236structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.9993438proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9992587protein_statecleaner02023-07-25T16:50:38ZDUMMY:open0.9993105proteincleaner02023-07-25T14:58:03ZPR:UHRF10.77143633protein_typecleaner02023-07-25T15:05:08ZMESH:histone0.98646134ptmcleaner02023-07-25T16:29:51ZMESH:ubiquitination0.579737protein_typecleaner02023-07-25T15:05:08ZMESH:histonestructure_elementSO:cleaner02023-07-25T15:01:28ZRING0.99918133proteincleaner02023-07-25T15:00:38ZPR:DNMT10.9989122proteincleaner02023-07-25T15:00:38ZPR:DNMT10.993705chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNAptmMESH:cleaner02023-07-25T15:05:57Zmethylation0.8369093protein_typecleaner02023-07-25T15:05:08ZMESH:histoneptmMESH:cleaner02023-07-25T16:11:22Zubiquitylationstructure_elementSO:cleaner02023-07-25T15:04:02ZSRA-Spacer0.995608evidencecleaner02023-07-25T16:45:53ZDUMMY:P(r) function0.99899787experimental_methodcleaner02023-07-25T16:41:43ZMESH:small-angle X-ray scattering0.9984654experimental_methodcleaner02023-07-25T16:41:45ZMESH:SAXS0.9987902complex_assemblycleaner02023-07-25T16:06:20ZGO:TTD–PHD–SRA–Spacer–hm-DNA0.99797595complex_assemblycleaner02023-07-25T16:06:09ZGO:TTD–PHD–SRA–Spacer0.9993412proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9992655protein_statecleaner02023-07-25T16:50:41ZDUMMY:open0.99916625protein_statecleaner02023-07-25T15:10:25ZDUMMY:presence of0.99755687chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNADISCUSSparagraph28833Many questions need to be further clarified. We have tried crystallizing more than three sub-constructs with and without DNA across over 1,200 crystallization conditions but failed to determine the structure of TTD–PHD–SRA–Spacer in the absence or presence of hm-DNA. Getting these structures would greatly help for understanding the hm-DNA-mediated regulation of UHRF1. In addition, this regulatory process should be further characterized using advanced techniques, such as single molecular measurement.0.9979419experimental_methodcleaner02023-07-25T16:41:49ZMESH:crystallizing0.95115554protein_statecleaner02023-07-25T16:30:30ZDUMMY:with0.9905099protein_statecleaner02023-07-25T16:30:32ZDUMMY:without0.9972832chemicalcleaner02023-07-25T16:30:27ZCHEBI:DNA0.99836916evidencecleaner02023-07-25T16:45:56ZDUMMY:structurecomplex_assemblyGO:cleaner02023-07-25T16:06:10ZTTD–PHD–SRA–Spacer0.9971886protein_statecleaner02023-07-25T16:30:35ZDUMMY:absence0.99712753protein_statecleaner02023-07-25T15:10:25ZDUMMY:presence of0.95390725chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.9982545evidencecleaner02023-07-25T16:45:59ZDUMMY:structures0.9149298chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.9990747proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9973283experimental_methodcleaner02023-07-25T16:41:59ZMESH:single molecular measurementDISCUSSparagraph29344Our previous studies show that phosphorylation at S639 within the Spacer disrupts interaction between UHRF1 and deubiquitylase USP7 and decreases UHRF1 stability in the M phase of the cell cycle. The Spacer was predicted to contain two nuclear localization signals, residues 581–600 and 648-670 (ref.). In this report, we found that the Spacer (i) binds to the TTD in the closed form of UHRF1 and inhibits its interaction with H3K9me3; (ii) facilitates hm-DNA recognition by the SRA and (iii) facilitates the interaction between the SRA and RFTSDNMT1. These findings together indicate that the Spacer plays a very important role in the dynamic regulation of UHRF1. When our manuscript was in preparation, Gelato et al. reported that binding of PI5P to the Spacer opens the closed conformation of UHRF1 and increases H3K9me3-binding affinity of the TTD. The result suggests that PI5P may facilitate the conformational change of UHRF1 induced by hm-DNA when UHRF1 is recruited to chromatin. In addition, mass-spectrometry analyses have identified several phosphorylation sites (S639, S651, S661) within the Spacer, suggesting that post-translational modification may add another layer of regulation of UHRF1 (refs).0.9963503ptmcleaner02023-07-25T15:27:39ZMESH:phosphorylation0.9995302residue_name_numbercleaner02023-07-25T16:07:15ZDUMMY:S6390.9990552structure_elementcleaner02023-07-25T14:57:06ZSO:Spacer0.9992318proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9987031protein_typecleaner02023-07-25T16:06:41ZMESH:deubiquitylase0.9992466proteincleaner02023-07-25T16:06:36ZPR:USP70.99921703proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9991099structure_elementcleaner02023-07-25T14:57:06ZSO:Spacer0.9739017structure_elementcleaner02023-07-25T16:32:08ZSO:nuclear localization signals0.9976179residue_rangecleaner02023-07-25T16:38:44ZDUMMY:581–6000.99757415residue_rangecleaner02023-07-25T16:38:47ZDUMMY:648-6700.99928963structure_elementcleaner02023-07-25T14:57:06ZSO:Spacerprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.99951804structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.99922633protein_statecleaner02023-07-25T14:59:20ZDUMMY:closed0.99931645proteincleaner02023-07-25T14:58:03ZPR:UHRF1protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:55:10ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:55:18ZK9me3chemicalCHEBI:cleaner02023-07-25T14:58:26Zhm-DNA0.7548684structure_elementcleaner02023-07-25T14:57:36ZSO:SRA0.58831096structure_elementcleaner02023-07-25T14:57:36ZSO:SRA0.9992194proteincleaner02023-07-25T16:02:13ZPR:RFTSDNMT10.99913114structure_elementcleaner02023-07-25T14:57:06ZSO:Spacer0.99928147proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9988167chemicalcleaner02023-07-25T15:03:00ZCHEBI:PI5P0.9990702structure_elementcleaner02023-07-25T14:57:06ZSO:Spacer0.99918395protein_statecleaner02023-07-25T14:59:20ZDUMMY:closed0.9993456proteincleaner02023-07-25T14:58:03ZPR:UHRF10.9901287evidencecleaner02023-07-25T15:36:49ZDUMMY:H3K9me3-binding affinity0.99946576structure_elementcleaner02023-07-25T14:57:20ZSO:TTD0.9987204chemicalcleaner02023-07-25T15:03:00ZCHEBI:PI5P0.9992894proteincleaner02023-07-25T14:58:04ZPR:UHRF10.7813921chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.9993051proteincleaner02023-07-25T14:58:04ZPR:UHRF10.99864024experimental_methodcleaner02023-07-25T16:42:02ZMESH:mass-spectrometry0.96276474sitecleaner02023-07-25T16:07:07ZSO:phosphorylation sites0.9995316residue_name_numbercleaner02023-07-25T16:07:14ZDUMMY:S6390.99952173residue_name_numbercleaner02023-07-25T15:26:00ZDUMMY:S6510.9995235residue_name_numbercleaner02023-07-25T16:07:21ZDUMMY:S6610.99915576structure_elementcleaner02023-07-25T14:57:06ZSO:Spacer0.99927586proteincleaner02023-07-25T14:58:04ZPR:UHRF1DISCUSSparagraph30560It has been well characterized that the SRA of UHRF1 preferentially recognizes hm-DNA through a base-flipping mechanism. Our study demonstrates that the Spacer markedly enhances the hm-DNA-binding affinity of the SRA and the deletion of the Spacer impairs heterochromatin localization of UHRF1, indicating that the Spacer is essential for recognition of hm-DNA in the context of full-length UHRF1. Interestingly, variant in methylation 1 (VIM1, a UHRF1 homologue in Arabidopsis) contains an equivalent spacer region, which was shown to be required for hm-DNA recognition by its SRA domain, suggesting a conserved regulatory mechanism in SRA domain-containing proteins. Intriguingly, UHRF2 (the only mammalian homologue of UHRF1) and UHRF1 show very high sequence similarities for all the domains but very low similarity for the Spacer (Supplementary Fig. 7c). Thus, although UHRF2 exhibits the histone- and hm-DNA-binding activities, the difference in the Spacer region may contribute to the functional differences between UHRF1 and UHRF2. This is also consistent with previous finding that UHRF2 is unable to replace UHRF1 to maintain the DNA methylation.0.9994406structure_elementcleaner02023-07-25T14:57:36ZSO:SRA0.9991404proteincleaner02023-07-25T14:58:04ZPR:UHRF10.9270676chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.9990067structure_elementcleaner02023-07-25T14:57:06ZSO:Spacer0.99293834evidencecleaner02023-07-25T14:56:50ZDUMMY:hm-DNA-binding affinity0.99931526structure_elementcleaner02023-07-25T14:57:36ZSO:SRA0.9863229experimental_methodcleaner02023-07-25T16:42:06ZMESH:deletion of0.9961428structure_elementcleaner02023-07-25T14:57:06ZSO:Spacer0.99889886proteincleaner02023-07-25T14:58:04ZPR:UHRF10.99849296structure_elementcleaner02023-07-25T14:57:06ZSO:Spacer0.84966046chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.99905974protein_statecleaner02023-07-25T15:09:01ZDUMMY:full-length0.9991301proteincleaner02023-07-25T14:58:04ZPR:UHRF10.9977554proteincleaner02023-07-25T16:07:45ZPR:variant in methylation 10.9988433proteincleaner02023-07-25T16:07:49ZPR:VIM10.9989505proteincleaner02023-07-25T14:58:04ZPR:UHRF10.9986185taxonomy_domaincleaner02023-07-25T16:07:57ZDUMMY:Arabidopsisstructure_elementSO:cleaner02023-07-25T14:57:06Zspacer0.8714142chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.99954695structure_elementcleaner02023-07-25T14:57:36ZSO:SRAstructure_elementSO:cleaner02023-07-25T14:57:36ZSRA0.9990758proteincleaner02023-07-25T16:08:25ZPR:UHRF20.9986889taxonomy_domaincleaner02023-07-25T15:00:05ZDUMMY:mammalian0.99895585proteincleaner02023-07-25T14:58:04ZPR:UHRF10.99911803proteincleaner02023-07-25T14:58:04ZPR:UHRF10.99908423structure_elementcleaner02023-07-25T14:57:06ZSO:Spacer0.9989396proteincleaner02023-07-25T16:08:26ZPR:UHRF2structure_elementSO:cleaner02023-07-25T14:57:06ZSpacer0.99909437proteincleaner02023-07-25T14:58:04ZPR:UHRF10.9990441proteincleaner02023-07-25T16:08:26ZPR:UHRF20.99901295proteincleaner02023-07-25T16:08:26ZPR:UHRF20.9990362proteincleaner02023-07-25T14:58:04ZPR:UHRF1chemicalCHEBI:cleaner02023-07-25T16:09:23ZDNAptmMESH:cleaner02023-07-25T15:05:57ZmethylationDISCUSSparagraph31717One of the key questions in the field of DNA methylation is why UHRF1 contains modules recognizing two repressive epigenetic marks: H3K9me3 (by TTD–PHD) and hm-DNA (by the SRA). Previous studies show that chromatin localization of UHRF1 is dependent on hm-DNA, whereas other studies indicate that histone H3K9me3 recognition and hm-DNA association are both required for UHRF1-mediated maintenance DNA methylation. However, little is known about the crosstalk between these two epigenetic marks within UHRF1. In this study, we provide an explanation. As shown in the proposed model, recognition of H3K9me3 by full-length UHRF1 is blocked to avoid its miss-localization to unmethylated genomic region, in which chromatin contains H3K9me3 (KD=4.61 μM) or H3K9me0 (KD=25.99 μM). We have shown that full-length UHRF1 and SRA–Spacer strongly bind to hm-DNA (0.35 and 0.49 μM, respectively) and the Spacer plays an important role in PCH localization (Fig. 5d). Therefore, genomic localization of UHRF1 is primarily determined by its recognition of hm-DNA, which allows UHRF1 to adopt an open form and promotes its histone tail recognition for proper genomic localization and function. As a result, when SRA–Spacer dissociates from hm-DNA and binds to DNMT1 with a currently unknown mechanism, UHRF1 may keep the complex associated with chromatin through the interaction between TTD–PHD and H3K9me3 (or PHD-H3), and make it possible for DNMT1 to target proper DNA substrate for methylation. This explanation agrees nicely with previous observations and clarifies the importance of coordinate recognition of H3K9me3 and hm-DNA by UHRF1 for maintenance DNA methylation.chemicalCHEBI:melaniev@ebi.ac.uk2023-08-08T12:55:32ZDNAptmMESH:cleaner02023-07-25T15:05:57Zmethylation0.9992513proteincleaner02023-07-25T14:58:04ZPR:UHRF1protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:56:23ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:56:16ZK9me30.99834013structure_elementcleaner02023-07-25T15:11:21ZSO:TTD–PHD0.99753475chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.97159886structure_elementcleaner02023-07-25T14:57:36ZSO:SRA0.9992951proteincleaner02023-07-25T14:58:04ZPR:UHRF10.9839018chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.74091005protein_typecleaner02023-07-25T15:05:08ZMESH:histoneprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:55:50ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:55:58ZK9me3chemicalCHEBI:cleaner02023-07-25T14:58:26Zhm-DNA0.999199proteincleaner02023-07-25T14:58:04ZPR:UHRF1chemicalCHEBI:cleaner02023-07-25T16:10:31ZDNAptmMESH:cleaner02023-07-25T15:05:57Zmethylation0.99930465proteincleaner02023-07-25T14:58:04ZPR:UHRF1protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:56:37ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:56:46ZK9me30.99916047protein_statecleaner02023-07-25T15:09:01ZDUMMY:full-length0.9992872proteincleaner02023-07-25T14:58:04ZPR:UHRF10.99787104protein_statecleaner02023-07-25T15:04:48ZDUMMY:unmethylatedprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:56:59ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:57:08ZK9me30.992324evidencecleaner02023-07-25T15:10:11ZDUMMY:KDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:57:25ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:57:35ZK9me00.9930274evidencecleaner02023-07-25T15:10:11ZDUMMY:KD0.99916273protein_statecleaner02023-07-25T15:09:01ZDUMMY:full-length0.9993338proteincleaner02023-07-25T14:58:04ZPR:UHRF10.99657935structure_elementcleaner02023-07-25T15:48:55ZSO:SRA–Spacer0.9956055chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.99895966structure_elementcleaner02023-07-25T14:57:06ZSO:Spacer0.99927944proteincleaner02023-07-25T14:58:04ZPR:UHRF10.9932789chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.9992743proteincleaner02023-07-25T14:58:04ZPR:UHRF10.9988243protein_statecleaner02023-07-25T16:50:46ZDUMMY:openprotein_typeMESH:cleaner02023-07-25T15:05:08Zhistone0.99772996structure_elementcleaner02023-07-25T15:48:55ZSO:SRA–Spacer0.9933903chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNAprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.9993279proteincleaner02023-07-25T15:00:38ZPR:DNMT10.9992974proteincleaner02023-07-25T14:58:04ZPR:UHRF10.9956915structure_elementcleaner02023-07-25T15:11:21ZSO:TTD–PHDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:57:54ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:58:04ZK9me30.966383structure_elementcleaner02023-07-25T14:57:50ZSO:PHD0.56240326protein_typecleaner02023-07-25T15:07:50ZMESH:H30.9991653proteincleaner02023-07-25T15:00:38ZPR:DNMT1chemicalCHEBI:cleaner02023-07-25T16:10:11ZDNAptmMESH:cleaner02023-07-25T15:05:57Zmethylationprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:58:18ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:58:27ZK9me30.99602073chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.9992939proteincleaner02023-07-25T14:58:04ZPR:UHRF1ptmMESH:cleaner02023-07-25T15:05:58ZmethylationDISCUSSparagraph33394UHRF1 is essential for maintenance DNA methylation through recruiting DNMT1 to DNA replication forks during S phase. This function is probably induced by a direct interaction between the SRA and RFTSDNMT1 (refs) or interaction between DNMT1 and ubiquitylation of histione tail. Recent study indicates that histone tail association of UHRF1 (by the PHD domain) is required for histone H3 ubiquitylation, which is dependent on ubiquitin ligase activity of the RING domain of UHRF1 (ref.). DNMT1 binds to ubiquitylated histone H3 and ubiquitylation is required for maintenance of DNA methylation in vivo. In this study, we found that both TTD and PHD are regulated by hm-DNA to recognize histone tail. Thus, the closed form UHRF1 may prevent miss localization of URHF1, whereas only the UHRF1 in open conformation (induced by hm-DNA) could properly binds to histone tail for ubiquitylation and subsequent DNA methylation.0.99912244proteincleaner02023-07-25T14:58:04ZPR:UHRF1chemicalCHEBI:cleaner02023-07-25T16:10:58ZDNAptmMESH:cleaner02023-07-25T15:05:58Zmethylation0.9992249proteincleaner02023-07-25T15:00:38ZPR:DNMT1chemicalCHEBI:cleaner02023-07-25T16:11:06ZDNA0.99181515structure_elementcleaner02023-07-25T14:57:36ZSO:SRA0.99929225proteincleaner02023-07-25T16:02:13ZPR:RFTSDNMT10.9993098proteincleaner02023-07-25T15:00:38ZPR:DNMT10.9939644ptmcleaner02023-07-25T16:11:21ZMESH:ubiquitylationprotein_typeMESH:cleaner02023-07-25T16:32:35Zhistioneprotein_typeMESH:cleaner02023-07-25T15:05:09Zhistone0.9993056proteincleaner02023-07-25T14:58:04ZPR:UHRF10.99944574structure_elementcleaner02023-07-25T14:57:50ZSO:PHD0.8996436protein_typecleaner02023-07-25T15:05:09ZMESH:histone0.9478336protein_typecleaner02023-07-25T15:07:50ZMESH:H30.99182045ptmcleaner02023-07-25T16:11:22ZMESH:ubiquitylation0.97545373protein_typecleaner02023-07-25T16:29:31ZMESH:ubiquitin ligasestructure_elementSO:cleaner02023-07-25T15:01:28ZRING0.99932575proteincleaner02023-07-25T14:58:04ZPR:UHRF10.9993137proteincleaner02023-07-25T15:00:38ZPR:DNMT1protein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.9985134protein_statecleaner02023-07-25T16:50:52ZDUMMY:ubiquitylated0.9693887protein_typecleaner02023-07-25T15:05:09ZMESH:histone0.948022protein_typecleaner02023-07-25T15:07:50ZMESH:H30.9429411ptmcleaner02023-07-25T16:11:22ZMESH:ubiquitylationchemicalCHEBI:cleaner02023-07-25T16:11:16ZDNAptmMESH:cleaner02023-07-25T15:05:58Zmethylation0.99955565structure_elementcleaner02023-07-25T14:57:21ZSO:TTD0.99952936structure_elementcleaner02023-07-25T14:57:50ZSO:PHD0.995536chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNAprotein_typeMESH:cleaner02023-07-25T15:05:09Zhistone0.99926776protein_statecleaner02023-07-25T14:59:20ZDUMMY:closed0.9993462proteincleaner02023-07-25T14:58:04ZPR:UHRF10.99938595proteincleaner02023-07-25T16:29:17ZPR:URHF10.9993699proteincleaner02023-07-25T14:58:04ZPR:UHRF10.99925655protein_statecleaner02023-07-25T16:50:55ZDUMMY:open0.99442863chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNAprotein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds toprotein_typeMESH:cleaner02023-07-25T15:05:09ZhistoneptmMESH:cleaner02023-07-25T16:11:22ZubiquitylationchemicalCHEBI:cleaner02023-07-25T16:11:33ZDNAptmMESH:cleaner02023-07-25T15:05:58ZmethylationDISCUSSparagraph34313Moreover, structural analyses of DNMT1–DNA and SRA–DNA complexes also indicate that it is impossible for DNMT1 to methylate the hm-DNA that UHRF1 binds to because of steric hindrance. In our in vitro assays, we could detect interaction between SRA–Spacer and RFTSDNMT1, but not the interaction between full-length UHRF1 and RFTSDNMT1 (Supplementary Fig. 8a,b and Fig. 5e). The results suggest that UHRF1 adopts multiple conformations. Binding of UHRF1 to hm-DNA may serve as a switch for its recruitment of DNMT1. The S phase-dependent interaction between UHRF1 and DNMT1 (refs) suggest that DNMT1 may also undergo conformation changes so that RFTSDNMT1 binds to UHRF1 and the catalytic domain of DNMT1 binds to hm-DNA for reaction.0.99893475experimental_methodcleaner02023-07-25T16:42:12ZMESH:structural analyses0.9986643complex_assemblycleaner02023-07-25T16:12:01ZGO:DNMT1–DNA0.9991562complex_assemblycleaner02023-07-25T16:12:07ZGO:SRA–DNA0.99922836proteincleaner02023-07-25T15:00:38ZPR:DNMT10.99494505chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.99907386proteincleaner02023-07-25T14:58:04ZPR:UHRF1protein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.9988796experimental_methodcleaner02023-07-25T16:42:15ZMESH:in vitro assays0.8854396structure_elementcleaner02023-07-25T15:48:55ZSO:SRA–Spacer0.9989078proteincleaner02023-07-25T16:02:13ZPR:RFTSDNMT10.9990818protein_statecleaner02023-07-25T15:09:01ZDUMMY:full-length0.99901927proteincleaner02023-07-25T14:58:04ZPR:UHRF10.9988921proteincleaner02023-07-25T16:02:13ZPR:RFTSDNMT10.99912745proteincleaner02023-07-25T14:58:04ZPR:UHRF10.99913675proteincleaner02023-07-25T14:58:04ZPR:UHRF10.99709946chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.99923027proteincleaner02023-07-25T15:00:38ZPR:DNMT10.9991431proteincleaner02023-07-25T14:58:04ZPR:UHRF10.9992536proteincleaner02023-07-25T15:00:38ZPR:DNMT10.9992206proteincleaner02023-07-25T15:00:38ZPR:DNMT10.9991217proteincleaner02023-07-25T16:02:13ZPR:RFTSDNMT1protein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.99914336proteincleaner02023-07-25T14:58:04ZPR:UHRF10.9992223structure_elementcleaner02023-07-25T16:32:43ZSO:catalytic domain0.99927205proteincleaner02023-07-25T15:00:38ZPR:DNMT1protein_stateDUMMY:cleaner02023-07-25T15:13:04Zbinds to0.99804133chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNAMETHODStitle_135052MethodsMETHODStitle_235060Protein expression and purificationMETHODSparagraph35096The ubiquitin-like domain (residues 1–133), TTD (residues 134–285), PHD (residues 307–366), TTD–PHD (residues 134–366), SRA (residues 414–617), SRA–Spacer (residues 414–674), Spacer (residues 587–674), RING (residues 675–793), UHRF1 (residues 1–793) and other mutants or truncations of human UHRF1 were sub-cloned in a pGEX-6p-1 derivative vector. The truncated Spacer (residues 627–674) used for NMR analyses was inserted into modified pRSF-Duet-1 vector. All the proteins were expressed in E. coli strain BL21 (DE3) and purified as described previously. In brief, the transformants were grown at 37 °C in 2X YT medium and induced by adding isopropyl-β-D-thiogalactopyranoside (IPTG) to 0.1 mM when the OD600 reached 0.6 and further incubated at 15 °C overnight. The cells were harvested and disrupted. After centrifugation, the supernatant of GST-tagged proteins was purified by GST affinity column (GE Healthcare) and the His-tagged truncated Spacer (residues 627–674) was purified by Nickel Nitrilotriacetic Acid affinity chromatography (GE Healthcare). The GST-tagged proteins used for GST pull-down experiment were eluted directly. The fusion proteins were digested with PreScission protease and further purified by ion exchange and gel filtration chromatography. The proteins were concentrated to 5–20 mg ml−1 for the following biochemical and structural analyses.METHODSparagraph36511To purify 15N- and 13C-labelled proteins, the transformants were grown in M9 medium containing 15N-labelled NH4Cl (1 g l−1) and 13C-labelled glucose (2 g l−1). The isotope-labelled TTD and truncated Spacer were purified as described above.METHODStitle_236763Pull-down experimentsMETHODSparagraph36785For GST pull-down assays, 15 μg GST-tagged proteins were incubated with 40 μg recombinant proteins in 500 μl pull-down buffer (20 mM HEPES-NaOH, pH 8.0, 100 mM NaCl, 5% glycerol and 0.1% Triton X-100) for 1 h at 4 °C. Glutathione resins (GE Healthcare) were washed six times with pull-down buffer then mixed with the proteins for 1 h at 4 °C. After washed three times with pull-down buffer, the bound proteins were analysed by SDS–PAGE Coomassie blue staining. For competitive pull-down experiments: purified proteins were pre-incubated with hemi-methylated-DNA (12 bp, upper strand: 5′-GGGCCXGCAGGG-3′, X=5-methyldeoxycytosine) at the indicated molar ratios for 10 min at 4 °C. For salt concentration-dependent pull-down experiments, the pull-down buffer contains 50, 100 or 150 mM NaCl, respectively.METHODSparagraph37626For histone peptide or hm-DNA pull-down, 1 μg biotinylated histone H3 peptide (residues 1–21) or hm-DNA (12-bp, upper strand 5′-GAGGCXGCCTGC-3′ and lower strand 5′-biotin-GCAGGCGGCCTC-3′, X=5-methyldeoxycytosine) were incubated with 20 μg wild type or mutants of UHRF1 proteins in 500 μl pull-down buffer (20 mM HEPES-NaOH, pH 8.0, 100 mM NaCl, 5% glycerol and 0.1% Triton X-100) for 1 h at 4 °C. The proteins were pre-incubated with hm-DNA (12- bp, upper strand: 5′-GGGCCXGCAGGG-3′, X=5-methyldeoxycytosine) or indicated H3 peptide, or binding buffer as a control, at 1:2 molar ratios for 10 min at 4 °C. Then, 20 μl streptavidin beads were washed six times with pull-down buffer and incubated with the mixture for 1 h at 4 °C. The bound proteins were analysed as described above. The results are summarized in Supplementary Fig. 10.METHODStitle_238508ITC measurementsMETHODSparagraph38525The binding affinity of protein/protein, protein/peptide or protein/DNA was measured by adding 0.05 mM protein in cell and titrated with 0.5 mM protein, peptide or hm-DNA (12 bp, upper strand: 5′-GGGCCXGCAGGG-3′, X=5-methyldeoxycytosine) in the syringe using iTC200 microcalorimeter (GE Healthcare) at 18 °C. For competition ITC experiments: the indicated proteins were pre-incubated with competitive peptide, protein or hm-DNA (at 1:2 molar ratio if not specified) for 10 min followed by ITC measurements as described above. Proteins, DNA and peptides were prepared within ITC buffer containing 10 mM HEPES, pH 8.0, 100 mM NaCl. The data were fitted by software Origin 7.0. All ITC results were summarized in Supplementary Table 2 and raw data were shown in Supplementary Fig. 9.METHODStitle_239325NMR titration assayMETHODSparagraph39345To determine the mole ratio of TTD versus Spacer peptide (residues 627–674) in the complex for NMR studies, NMR stepwise titration assay was performed at 20 °C in a PBS buffer supplemented with 0.01% NaN3, pH 7.4 and 10% D2O. The Spacer peptide was added into 15N-labelled TTD solution with an increasing molar ratio of TTD/Spacer as follows: 1:0.0, 1:0.2, 1:0.4, 1:0.6, 1:0.8, 1:1.2 and 1:1.5. The 1H–15N heteronuclear single-quantum correlation (HSQC) spectra of the TTD were collected after each addition.METHODStitle_239861NMR spectroscopy and analysisMETHODSparagraph39891Two NMR samples were prepared in a mole ratio of 1:1.2 (TTD/Spacer). One is 0.7 mM uniformly 13C/15N-labelled TTD in complex with unlabelled Spacer peptide in NMR buffer (PBS buffer, 0.01% NaN3, pH 7.4 and 10% D2O). The other is 0.5 mM 15N-labelled Spacer peptide mixed with unlabelled TTD protein. All NMR experiments were performed at 20 °C on a Varian Unity Inova 600 NMR spectrometer equipped with a triple resonances cryoprobe and pulsed field gradients. The standard suite of experiments for assigning the 1H, 13C and 15N backbone, determining the side-chain chemical shifts of the TTD in complex with the Spacer peptide and collecting the Nuclear Overhauser effect (NOE)-based distance restraints were measured, including two-dimensional (2D) 13C-edited HSQC and 15N-edited HSQC; three-dimensional (3D) HNCA, HNCO, HN(CO)CA, HNCACB, CBCA(CO)NH, 15N-resolved HSQC-total correlation spectroscopy (TOCSY) and 13C-resolved HSQC-TOCSY in both aliphatic and aromatic regions; 15N-resolved HSQC-NOESY; 13C-resolved HSQC-NOESY for both aliphatic and aromatic resonances and 2D hbcbcgcdceheA and hbcbcgcdhdA spectra for the correlation of Cβ and Hδ or Hɛ in the aromatic ring that is used for aromatic proton assignment. The NMR signals of bound TTD were assigned according to the previously report. The proton NMR signals of the bound Spacer peptide were assigned by analysing the 2D 13C-filtered, 15N-filtered and J-resolved NOE spectroscopy (NOESY) and TOCSY spectra recorded for the 13C- and 15N-labelled protein with the unlabelled Spacer peptide and the 2D 1H–1H COSY, NOESY and TOCSY spectra recorded for the unlabelled free Spacer peptide, and 15N-edited HSQC, 3D 15N-resolved HSQC-TOCSY for the 15N-labelled Spacer in complex with the TTD protein in the NMR buffer described above, respectively. The intermolecular NOEs between the labelled protein and the unlabelled Spacer peptide were obtained by analysing the 3D 13C-F1-edited and 13C/15N-F3-filtered NOESY spectra. The spectra were processed with the NMRPipe programme and analysed using Sparky 3 (http://www.cgl.ucsf.edu/home/sparky/).METHODStitle_242002Determining the NMR structureMETHODSparagraph42032The calculations were performed using a standard simulated annealing protocol implemented in the XPLOR-2.29 programme (NIH version). The inter-proton distance restraints derived from the NOE intensities were grouped into three distance ranges, namely 1.8–2.9, 1.8–3.5and 1.8–6.0 Å, which corresponds to strong, medium and weak NOEs, respectively. The dihedral angles phi and psi were derived from the backbone chemical shifts (HN, HA, CO and CA) using the programme TALOS. The hydrogen-bond constraints were generated based on the observed NOE pattern between anti-β-sheets in the TTD domain, confirmed by H-D exchange experiments, and used in structural calculation. A total of ten iterations were performed (50 structures in the initial eight iterations). In total, 100 structures were computed during the last two iterations, and the 20 conformers with the lowest energy were used to represent the 3D structures. The conformers of these bundles (TTD in complex with the Spacer peptide) do not violate the following constraints: NOE >0.3 Å and dihedral angle >3o. The entire structure statistics were evaluated with PROCHECK and PROCHECK-NMR and are summarized in Supplementary Table 3. All of the structure figures were generated using the PyMOL and MOLMOL programmes.METHODStitle_243317Electrophoretic mobility-shift assayMETHODSparagraph43354A 6-carboxy-fluorescein (FAM)-labelled primer, 5′-CCATGCGCTGAC-3′, was annealed to a primer 5′-GTCAGXGCATGG-3′ (X=5-methyldeoxycytosine). The hemi-methylated double-strand DNA was used in both electrophoretic mobility-shift assay and FP assays. 50 nM FAM-hm-DNA (1 pmole per lane) was pre-incubated with indicated amount of proteins in reaction buffer (20 mM HEPES, pH 7.5, 100 mM NaCl, 8% glycerol and 1 mM DTT) for 20 min on ice. The samples were subjected to a 10% polyacrylamide gel electrophoresis and run in 0.5 × Tris-borate-EDTA buffer at 100 V for 1 h at 4 °C. The results were visualized on Tanon-5200 Chemiluminescent Imaging System (Tanon Science & Technology Co., Ltd).METHODStitle_244065FP measurementsMETHODSparagraph44081The 12-bp FAM-labelled hm-DNA (as described above) was incubated with increasing amount of indicated proteins for 20 min at 25 °C in reaction buffer containing 20 mM HEPES, pH 7.5, 175 mM NaCl, 8% glycerol and 1 mM DTT. FP measurements were performed at 25 °C on Synergy 4 Microplate Reader (BioTek). The 16-bp and 20-bp FAM-labelled hm-DNA (lower strand: 5′-GTGTCAGXGCATGGCC-3′ and 5′-CCGTGTCAGXGCATGGCCAT-3′, respectively. X=5-methyldeoxycytosine) were used in the FP experiment to test the effect of DNA length on the protein/DNA interaction. All experiments were performed in triplicate. The curves were fitted by GraphPad Prism 5. For salt concentration-dependent FP experiments, the reaction buffer contains 50 mM or 150 mM NaCl, respectively.METHODStitle_244857Crystallization and data collectionMETHODSparagraph44893Crystals of SRA–Spacer in complex with an 18-bp hm-DNA (upper strand: 5′-CATCGTCCCTGCGGGCCC-3′, lower strand: 5′-GGGCCXGCAGGGACGATG-3′. X=5-methyldeoxycytosine) were grown at 18 °C using the hanging drop vapour diffusion method by mixing an equal volume of protein–DNA complex and crystallization buffer containing 12% PEG 3350, 45 mM citric acid/55 mM BIS-TRIS propane (pH 6.9). Protein and hm-DNA were mixed at the molar ratio of 1:1.5 and incubated for 0.5 h on ice before crystallization. Crystals were flash frozen in a cold nitrogen stream at −173 °C. All data sets were collected on beamline BL17U at the SSRF (Shanghai Synchrotron Radiation Facility, China). The data were processed using the programme HKL2000 (ref.).METHODStitle_245646Structure determinationMETHODSparagraph45670The structure of SRA–Spacer–hm-DNA complex was determined by molecular replacement using structure of the SRA (PDB:3BI7) as a searching model. Rotation and translation function searches were performed with the programme PHASER. The model was manually built with COOT. All refinements were performed using the refinement module phenix.refine of PHENIX package. The model quality was checked with the PROCHECK programme and all structure figures were generated by PyMol.METHODStitle_246143Cell culture, transient transfection and images captureMETHODSparagraph46199NIH3T3 cells were obtained from the Shanghai Institute of Biochemistry and Cell Biology. Wild type and mutants of UHRF1 were sub-cloned into pEGFP-C1 vector. Transient transfections of NIH3T3 cells were carried out using Lipofectamine 2000 (Invitrogen). The NIH3T3 cells were grown on glass coverslips and harvested in 36 h after transfection. The images were acquired and examined as previously described. Briefly, cells were fixed with 4% paraformaldehyde for 25 min, then washed with PBS three times. Coverslips were mounted with Antifade reagent containing DAPI (Molecular Probes) on slides and examined with a confocal microscopy.METHODStitle_246839SAXS measurementsMETHODSparagraph46857SAXS measurements were performed with Anton Paar SAXSess mc2 instrument with linecolimation and charge-coupled-device detection. The X-ray wavelength was 1.5418 Å (CuKα), the sample to detector was 306.8 mm and the sample slit width was 10 mm. Each sample was prepared in 300 μl solution contained 150 mM NaCl, 10 mM HEPES, pH=8.0, 5 mM DTT and 5% glycerol. For the hm-DNA-bound form, the protein was pre-incubated with hm-DNA (12-bp, upper strand: 5′-GGGCCmCGCAGGG-3′, mC=5-methyldeoxycytosine) at 1:1.2 molar ratio for 10 min on ice. To correct for interparticle interference, the data of protein sample were collected twice and each time for 1 h. The solution containing no protein sample was also tested as background.METHODSparagraph47605The initial data were first processed using SAXSquant and the further analysis with ATSAS software. The SAXS data were only analysed these were collected in the first hour because there was no time effect on the samples. The radius of gyration Rg was estimated from primus. The distance distribution function P(r) was calculated in PCG package. The maximum particle dimension Dmax was estimated from the P(r) function as the r for which P(r)=0.METHODStitle_148050Additional informationMETHODSparagraph48073Accession codes: The coordinate and structure factor for the TTD–Spacer complex structure have been deposited in the Protein Data Bank under accession code 5IAY. The chemical shift assignment of TTD–Spacer was deposited with BMRB ADIT-NMR online deposition system under the accession number 30019.METHODSparagraph48375How to cite this article: Fang, J. et al. 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C.T., W.L., C.C. and Jie.W. helped in the data analysis. J.F., H.Y. and Y.X. analysed the data and wrote the manuscript. Y.X. supervised the project.ncomms11197-f1.jpgf1FIGfig_title_caption55093Hm-DNA facilities histione tails recognition by full-length UHRF1.chemicalCHEBI:cleaner02023-07-25T14:58:26ZHm-DNAprotein_typeMESH:cleaner02023-07-25T16:33:30Zhistione0.9991283protein_statecleaner02023-07-25T15:09:01ZDUMMY:full-length0.99892884proteincleaner02023-07-25T14:58:05ZPR:UHRF1ncomms11197-f1.jpgf1FIGfig_caption55160(a) Colour-coded domain structure of human UHRF1. The boundaries of the domains are indicated with the numbers representing the amino-acid positions. Note that the conserved motif (green background) of the Linker (residues 286–306) and the Spacer (residues 587–674) bind to the TTD in a similar manner (Fig. 3b). (b) Hm-DNA facilities histone H3 and H3K9me3 recognition by UHRF1. Purified full-length UHRF1 was incubated with biotinylated H3 (1–21) or H3K9me3 (1–21) peptides in the presence or absence of hm-DNA (molar ratio UHRF1/hm-DNA=1:2). The mixture was immobilized onto streptavidin Sepharose beads. The bound proteins were analysed in SDS–PAGE followed by Coomassie blue staining. Sequences of the peptides are indicated in Supplementary Table 1. (c) Histone peptides do not affect hm-DNA-binding affinity of UHRF1. Full-length UHRF1 was incubated with biotinylated hm-DNA in the presence or absence of H3 (1–17) or H3K9me3 (1–17) peptides and analysed as in b. (d,e) Superimposed ITC enthalpy plots for binding of H3K9me3 peptide (1–17) to TTD–PHD and full-length UHRF1 (d), and H3 peptide (1–17) to the PHD and full-length UHRF1 (e). The estimated binding affinities (KD) are listed. The samples in the syringe (designated Sy hereafter) and cell are indicated.0.9987324speciescleaner02023-07-25T16:39:11ZMESH:human0.99917275proteincleaner02023-07-25T14:58:05ZPR:UHRF10.9983405protein_statecleaner02023-07-25T16:51:01ZDUMMY:conserved0.9991084structure_elementcleaner02023-07-25T15:34:29ZSO:Linker0.9964264residue_rangecleaner02023-07-25T16:38:52ZDUMMY:286–3060.99924755structure_elementcleaner02023-07-25T14:57:07ZSO:Spacer0.99628085residue_rangecleaner02023-07-25T16:38:56ZDUMMY:587–6740.9995442structure_elementcleaner02023-07-25T14:57:22ZSO:TTD0.9703517chemicalcleaner02023-07-25T14:58:26ZCHEBI:Hm-DNAprotein_typeMESH:cleaner02023-07-25T15:05:09Zhistone0.48119324protein_typecleaner02023-07-25T15:07:50ZMESH:H3protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T13:02:11ZH30.9991829proteincleaner02023-07-25T14:58:05ZPR:UHRF10.9990461protein_statecleaner02023-07-25T15:09:01ZDUMMY:full-length0.99922454proteincleaner02023-07-25T14:58:05ZPR:UHRF10.87862474protein_statecleaner02023-07-25T16:51:05ZDUMMY:biotinylated0.91468906protein_typecleaner02023-07-25T15:07:50ZMESH:H3residue_rangeDUMMY:melaniev@ebi.ac.uk2023-08-08T13:03:21Z1–21protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T13:02:23ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T13:02:42ZK9me3residue_rangeDUMMY:melaniev@ebi.ac.uk2023-08-08T13:03:32Z1–21protein_stateDUMMY:cleaner02023-07-25T15:50:14Zabsence of0.99181557chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.99652356proteincleaner02023-07-25T14:58:05ZPR:UHRF10.96265155chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNA0.9976464experimental_methodcleaner02023-07-25T16:42:52ZMESH:SDS–PAGEprotein_typeMESH:cleaner02023-07-25T15:05:09ZHistone0.85392904evidencecleaner02023-07-25T14:56:51ZDUMMY:hm-DNA-binding affinity0.99919266proteincleaner02023-07-25T14:58:05ZPR:UHRF10.9990065protein_statecleaner02023-07-25T15:09:01ZDUMMY:Full-length0.9992055proteincleaner02023-07-25T14:58:05ZPR:UHRF10.8146759experimental_methodcleaner02023-07-25T16:42:55ZMESH:incubated with0.57842284protein_statecleaner02023-07-25T16:51:08ZDUMMY:biotinylated0.99434835chemicalcleaner02023-07-25T14:58:26ZCHEBI:hm-DNAprotein_stateDUMMY:cleaner02023-07-25T15:50:14Zabsence of0.9817586protein_typecleaner02023-07-25T15:07:50ZMESH:H3residue_rangeDUMMY:cleaner02023-07-25T16:23:35Z1–17protein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:59:02ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:59:11ZK9me3residue_rangeDUMMY:cleaner02023-07-25T16:23:36Z1–170.99561787experimental_methodcleaner02023-07-25T16:42:58ZMESH:ITC0.96477485evidencecleaner02023-07-25T16:46:04ZDUMMY:enthalpy plotsprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:59:33ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:59:26ZK9me3residue_rangeDUMMY:cleaner02023-07-25T16:23:36Z1–17structure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHD0.99911135protein_statecleaner02023-07-25T15:09:01ZDUMMY:full-length0.9992053proteincleaner02023-07-25T14:58:05ZPR:UHRF10.9958145protein_typecleaner02023-07-25T15:07:50ZMESH:H30.6578081residue_rangecleaner02023-07-25T16:23:36ZDUMMY:1–170.99953353structure_elementcleaner02023-07-25T14:57:50ZSO:PHD0.99913436protein_statecleaner02023-07-25T15:09:01ZDUMMY:full-length0.9992557proteincleaner02023-07-25T14:58:05ZPR:UHRF10.99829507evidencecleaner02023-07-25T16:46:09ZDUMMY:binding affinities0.99740285evidencecleaner02023-07-25T15:10:11ZDUMMY:KDncomms11197-f2.jpgf2FIGfig_title_caption56452Intramolecular interactions inhibit histone recognition by UHRF1.protein_typeMESH:cleaner02023-07-25T15:05:09Zhistone0.9990262proteincleaner02023-07-25T14:58:05ZPR:UHRF1ncomms11197-f2.jpgf2FIGfig_caption56518(a) GST pull-down assays for the intramolecular interactions. The isolated domains of UHRF1 were incubated with GST-tagged TTD or PHD immobilized on glutathione resin. The bound proteins were analysed by SDS–PAGE and Coomassie blue staining. (b,d) Superimposed ITC enthalpy plots for the intramolecular interactions of isolated UHRF1 domains. The estimated binding affinities (KD) were listed. ND, not detectable. (c) Superimposed ITC enthalpy plots for the binding of H3K9me3 to TTD–PHD in the absence or presence of the Spacer (molar ratio TTD–PHD/Spacer=1:2). (e) Superimposed ITC enthalpy plots for the binding of H3 to PHD–SRA or PHD in the absence or presence of the SRA (molar ratio PHD/SRA=1:1 or 1:2). ND, not determined; Sy., syringe.0.9989753experimental_methodcleaner02023-07-25T16:24:11ZMESH:GST pull-down assays0.9977849proteincleaner02023-07-25T14:58:05ZPR:UHRF10.97791547experimental_methodcleaner02023-07-25T16:43:03ZMESH:incubated0.9943831protein_statecleaner02023-07-25T16:24:19ZDUMMY:GST-tagged0.99942833structure_elementcleaner02023-07-25T14:57:22ZSO:TTD0.9994708structure_elementcleaner02023-07-25T14:57:50ZSO:PHD0.9921228experimental_methodcleaner02023-07-25T16:43:06ZMESH:SDS–PAGE0.9880918experimental_methodcleaner02023-07-25T16:43:09ZMESH:ITC0.9660795evidencecleaner02023-07-25T16:46:13ZDUMMY:enthalpy plots0.9971623proteincleaner02023-07-25T14:58:05ZPR:UHRF10.9986558evidencecleaner02023-07-25T16:46:22ZDUMMY:binding affinities0.99723214evidencecleaner02023-07-25T15:10:11ZDUMMY:KD0.9923856experimental_methodcleaner02023-07-25T16:43:23ZMESH:ITC0.981375evidencecleaner02023-07-25T16:46:16ZDUMMY:enthalpy plotsprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T12:59:50ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T12:59:58ZK9me3structure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHD0.99501556protein_statecleaner02023-07-25T16:51:14ZDUMMY:absence0.99276066protein_statecleaner02023-07-25T15:10:25ZDUMMY:presence of0.9653634structure_elementcleaner02023-07-25T14:57:07ZSO:Spacerstructure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHD0.9408276structure_elementcleaner02023-07-25T14:57:07ZSO:Spacer0.9922605experimental_methodcleaner02023-07-25T16:43:27ZMESH:ITC0.98671687evidencecleaner02023-07-25T16:46:18ZDUMMY:enthalpy plots0.5302331protein_typecleaner02023-07-25T15:07:50ZMESH:H3structure_elementSO:cleaner02023-07-25T15:47:40ZPHD–SRA0.9993455structure_elementcleaner02023-07-25T14:57:50ZSO:PHD0.99725914protein_statecleaner02023-07-25T16:51:17ZDUMMY:absence0.9969782protein_statecleaner02023-07-25T15:10:25ZDUMMY:presence of0.9905939structure_elementcleaner02023-07-25T14:57:37ZSO:SRA0.99839264structure_elementcleaner02023-07-25T14:57:50ZSO:PHD0.98487955structure_elementcleaner02023-07-25T14:57:37ZSO:SRAncomms11197-f3.jpgf3FIGfig_title_caption57271NMR structure of the TTD bound to the Spacer.0.9986047experimental_methodcleaner02023-07-25T16:43:31ZMESH:NMR0.998346evidencecleaner02023-07-25T16:46:27ZDUMMY:structure0.99939525structure_elementcleaner02023-07-25T14:57:22ZSO:TTDprotein_stateDUMMY:cleaner02023-07-25T15:10:33Zbound to0.9795172structure_elementcleaner02023-07-25T14:57:07ZSO:Spacerncomms11197-f3.jpgf3FIGfig_caption57317(a) Ribbon representation of TTD–Spacer structure. N- and C-termini of the Spacer are indicated. The TTD is coloured in green, and the Spacer is coloured in yellow. The colour scheme is used the all the structure figures. (b) Superimposition of TTD–Spacer and TTD–PHD–H3K9me3 (4GY5.PDB) structures shown in ribbon representations. The TTD is coloured in green and the Spacer in yellow in TTD–Spacer structure. TTD–PHD–H3K9me3 complex is coloured in grey, and the PHD and H3K9me3 are omitted for simplicity. Residues for the interactions are shown in stick representation. (c) Electrostatic potential surface representation of the TTD with the Spacer shown in ribbon representation. The critical residues on the Spacer for the interaction are shown in stick representation. (d) Close-up view of TTD–Spacer interaction. Critical residues for the interaction are shown in stick representation. Hydrogen bonds are indicated as dashed lines. (e–g) Superimposed ITC enthalpy plots for the interaction between the Spacer and the TTD (or TTD–PHD) with the estimated binding affinity (KD) indicated. Wild-type and mutant proteins for the measurements are indicated. (h) GST pull-down assays for the intramolecular interactions. The wild-type or indicated truncations of UHRF1 were incubated with GST-tagged TTD, Linker or Spacer. The mixtures were immobilized on glutathione resin. The bound proteins were analysed by SDS–PAGE and Coomassie blue staining. ND, not determined; Sy., syringe.0.99809724structure_elementcleaner02023-07-25T15:17:21ZSO:TTD–Spacer0.8751053evidencecleaner02023-07-25T16:46:30ZDUMMY:structure0.9955915structure_elementcleaner02023-07-25T14:57:07ZSO:Spacer0.99941146structure_elementcleaner02023-07-25T14:57:22ZSO:TTD0.99854636structure_elementcleaner02023-07-25T14:57:07ZSO:Spacer0.988893experimental_methodcleaner02023-07-25T16:25:01ZMESH:Superimposition0.9639978structure_elementcleaner02023-07-25T15:17:21ZSO:TTD–Spacer0.99869716complex_assemblycleaner02023-07-25T15:22:29ZGO:TTD–PHD–H3K9me30.5059854evidencecleaner02023-07-25T16:46:33ZDUMMY:structures0.99940574structure_elementcleaner02023-07-25T14:57:22ZSO:TTD0.99869233structure_elementcleaner02023-07-25T14:57:07ZSO:Spacer0.99854136structure_elementcleaner02023-07-25T15:17:21ZSO:TTD–Spacer0.8394155evidencecleaner02023-07-25T16:46:36ZDUMMY:structure0.99902695complex_assemblycleaner02023-07-25T15:22:29ZGO:TTD–PHD–H3K9me30.9988858structure_elementcleaner02023-07-25T14:57:51ZSO:PHDprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T13:00:15ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T13:00:24ZK9me30.99935716structure_elementcleaner02023-07-25T14:57:22ZSO:TTD0.9981571structure_elementcleaner02023-07-25T14:57:07ZSO:Spacer0.9972881structure_elementcleaner02023-07-25T14:57:07ZSO:Spacerstructure_elementSO:cleaner02023-07-25T15:17:21ZTTD–Spacer0.9962218bond_interactioncleaner02023-07-25T15:24:59ZMESH:Hydrogen bonds0.99756014experimental_methodcleaner02023-07-25T16:43:38ZMESH:ITC0.9872527evidencecleaner02023-07-25T16:46:39ZDUMMY:enthalpy plots0.9953733structure_elementcleaner02023-07-25T14:57:07ZSO:Spacer0.9989366structure_elementcleaner02023-07-25T14:57:22ZSO:TTD0.9958517structure_elementcleaner02023-07-25T15:11:21ZSO:TTD–PHD0.99859107evidencecleaner02023-07-25T15:10:18ZDUMMY:binding affinity0.9974821evidencecleaner02023-07-25T15:10:11ZDUMMY:KD0.9990232protein_statecleaner02023-07-25T15:34:11ZDUMMY:Wild-typeprotein_stateDUMMY:cleaner02023-07-25T16:00:50Zmutant0.99896955experimental_methodcleaner02023-07-25T16:26:12ZMESH:GST pull-down assays0.9991037protein_statecleaner02023-07-25T15:34:11ZDUMMY:wild-type0.99932194proteincleaner02023-07-25T14:58:05ZPR:UHRF10.9956762protein_statecleaner02023-07-25T16:26:25ZDUMMY:GST-tagged0.9994342structure_elementcleaner02023-07-25T14:57:22ZSO:TTD0.99938715structure_elementcleaner02023-07-25T15:34:29ZSO:Linker0.99925107structure_elementcleaner02023-07-25T14:57:07ZSO:Spacer0.99821776experimental_methodcleaner02023-07-25T16:43:43ZMESH:SDS–PAGEncomms11197-f4.jpgf4FIGfig_title_caption58820Hm-DNA impairs the intramolecular interaction of UHRF1 and facilitates its histone recognition.0.9944139chemicalcleaner02023-07-25T14:58:27ZCHEBI:Hm-DNA0.9990503proteincleaner02023-07-25T14:58:05ZPR:UHRF1protein_typeMESH:cleaner02023-07-25T15:05:09Zhistonencomms11197-f4.jpgf4FIGfig_caption58916(a) Hm-DNA impairs the intramolecular interaction of PHD–SRA. The SRA was incubated with GST-tagged PHD in the presence of increasing concentrations of hm-DNA and immobilized on glutathione resin. The bound proteins were analysed in SDS–PAGE and Coomassie blue staining (left) and quantified by band densitometry (right). Error bars, s.d. for triplicate experiments. (b) Purified fragments of UHRF1 were analysed by histone peptide (H3K9me0) pull-down assay as described in Fig. 1b. (c) Hm-DNA impairs the intramolecular interaction of TTD–Spacer. SRA–Spacer was incubated with GST-tagged TTD–PHD or TTD in the presence of increasing concentrations of hm-DNA and analysed in pull-down experiment as described in a. The quantified band densitometries are indicated below the Coomassie blue staining. Error bars, s.d. for triplicate experiments. (d) Histone peptide pull-down assay using UHRF1 mutants as indicated. The assays were performed in the presence (+DTT) or absence (−DTT) of 15 mM DTT.0.9981992chemicalcleaner02023-07-25T14:58:27ZCHEBI:Hm-DNAstructure_elementSO:cleaner02023-07-25T15:47:40ZPHD–SRA0.99943036structure_elementcleaner02023-07-25T14:57:37ZSO:SRA0.98517853experimental_methodcleaner02023-07-25T16:43:49ZMESH:incubated0.9960082protein_statecleaner02023-07-25T16:51:22ZDUMMY:GST-tagged0.9994848structure_elementcleaner02023-07-25T14:57:51ZSO:PHDprotein_stateDUMMY:cleaner02023-07-25T15:10:25Zpresence of0.9988224chemicalcleaner02023-07-25T14:58:27ZCHEBI:hm-DNA0.9975583experimental_methodcleaner02023-07-25T16:44:14ZMESH:SDS–PAGEexperimental_methodMESH:cleaner02023-07-25T16:44:05ZCoomassie blue staining0.97438693experimental_methodcleaner02023-07-25T16:44:09ZMESH:band densitometry0.9990325proteincleaner02023-07-25T14:58:05ZPR:UHRF10.994632experimental_methodcleaner02023-07-25T16:27:22ZMESH:histone peptideprotein_typeMESH:melaniev@ebi.ac.uk2023-08-08T13:00:40ZH3ptmMESH:melaniev@ebi.ac.uk2023-08-08T13:00:52ZK9me00.99860966experimental_methodcleaner02023-07-25T16:27:25ZMESH:pull-down assay0.9979406chemicalcleaner02023-07-25T14:58:27ZCHEBI:Hm-DNAstructure_elementSO:cleaner02023-07-25T15:17:21ZTTD–Spacerstructure_elementSO:cleaner02023-07-25T15:48:56ZSRA–Spacer0.9862408experimental_methodcleaner02023-07-25T16:44:21ZMESH:incubated0.9954321protein_statecleaner02023-07-25T16:51:25ZDUMMY:GST-taggedstructure_elementSO:cleaner02023-07-25T15:11:21ZTTD–PHD0.9994454structure_elementcleaner02023-07-25T14:57:22ZSO:TTDprotein_stateDUMMY:cleaner02023-07-25T15:10:25Zpresence of0.99866724chemicalcleaner02023-07-25T14:58:27ZCHEBI:hm-DNA0.9964418experimental_methodcleaner02023-07-25T16:44:24ZMESH:pull-down experiment0.6687745experimental_methodcleaner02023-07-25T16:47:04ZMESH:band densitometriesexperimental_methodMESH:cleaner02023-07-25T16:47:18ZCoomassie blue staining0.99877673experimental_methodcleaner02023-07-25T16:27:38ZMESH:Histone peptide pull-down assay0.998818proteincleaner02023-07-25T14:58:05ZPR:UHRF10.9971113protein_statecleaner02023-07-25T16:00:01ZDUMMY:mutants0.9741964chemicalcleaner02023-07-25T15:51:51ZCHEBI:DTTchemicalCHEBI:cleaner02023-07-25T15:51:51ZDTT0.9975981chemicalcleaner02023-07-25T15:51:51ZCHEBI:DTTncomms11197-f5.jpgf5FIGfig_title_caption59925The Spacer facilitates hm-DNA–SRA interaction and DNMT1–UHRF1 interaction.0.99668473structure_elementcleaner02023-07-25T14:57:07ZSO:Spacer0.97400206chemicalcleaner02023-07-25T14:58:27ZCHEBI:hm-DNA0.9543789structure_elementcleaner02023-07-25T14:57:37ZSO:SRA0.90869576complex_assemblycleaner02023-07-25T16:30:45ZGO:DNMT1–UHRF1ncomms11197-f5.jpgf5FIGfig_caption60004(a) Superimposed ITC enthalpy plots for hm-DNA-binding affinities of the SRA, the Spacer and SRA–Spacer. (b,c) Superimposed fluorescence polarization (FP) plots for hm-DNA-binding affinities of truncations or full-length UHRF1. The estimated binding affinities (KD) are listed above. (d) Subcellular localization of GFP-tagged wild-type or indicated mutants of UHRF1 in NIH3T3 cells. The percentages of cells showing co-localization with DAPI foci were counted from at least 100 cells and shown on the left of the corresponding representative confocal microscopy. The experiment was repeated three times in the same condition. Scale bar, 5 μm. (e) GST pull-down experiment for the interactions between wild-type or truncations of UHRF1 and RFTSDNMT1 as described in Fig. 2a. (f) Working model for hm-DNA-mediated conformational changes of UHRF1, as described in the Discussion. ND, not determined; Sy., syringe.0.9923235experimental_methodcleaner02023-07-25T16:28:50ZMESH:ITC0.9836543evidencecleaner02023-07-25T16:47:28ZDUMMY:enthalpy plots0.9968479evidencecleaner02023-07-25T15:53:51ZDUMMY:hm-DNA-binding affinities0.99944144structure_elementcleaner02023-07-25T14:57:37ZSO:SRA0.9984194structure_elementcleaner02023-07-25T14:57:07ZSO:Spacerstructure_elementSO:cleaner02023-07-25T15:48:56ZSRA–SpacerevidenceDUMMY:cleaner02023-07-25T16:28:30Zfluorescence polarization (FP) plots0.99808145evidencecleaner02023-07-25T15:53:51ZDUMMY:hm-DNA-binding affinities0.99890304protein_statecleaner02023-07-25T15:09:01ZDUMMY:full-length0.99899775proteincleaner02023-07-25T14:58:05ZPR:UHRF10.9986728evidencecleaner02023-07-25T16:47:34ZDUMMY:binding affinities0.9979559evidencecleaner02023-07-25T15:10:11ZDUMMY:KD0.9150217protein_statecleaner02023-07-25T15:59:47ZDUMMY:GFP-tagged0.9991537protein_statecleaner02023-07-25T15:34:11ZDUMMY:wild-typeprotein_stateDUMMY:cleaner02023-07-25T16:00:01Zmutants0.99895537proteincleaner02023-07-25T14:58:05ZPR:UHRF1chemicalCHEBI:cleaner02023-07-25T16:00:11ZDAPI0.9746206experimental_methodcleaner02023-07-25T16:28:45ZMESH:confocal microscopy0.99787396experimental_methodcleaner02023-07-25T16:28:43ZMESH:GST pull-down experiment0.9991854protein_statecleaner02023-07-25T15:34:11ZDUMMY:wild-type0.66345346experimental_methodcleaner02023-07-25T16:28:47ZMESH:truncations0.99900025proteincleaner02023-07-25T14:58:05ZPR:UHRF10.9981184proteincleaner02023-07-25T16:02:13ZPR:RFTSDNMT10.9931757chemicalcleaner02023-07-25T14:58:27ZCHEBI:hm-DNA0.9990509proteincleaner02023-07-25T14:58:05ZPR:UHRF1