PMC20201216pmc.key4968113CC BY-NCno00A. TEPLYAKOV ET AL. MABS10.1080/19420862.2016.1190060496811327210805119006010456Antibody structure CDR canonical structure CDR H3 phage library VH:VL packingThis is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.1063surname:Teplyakov;given-names:Alexeysurname:Obmolova;given-names:Galinasurname:Malia;given-names:Thomas J.surname:Luo;given-names:Jinquansurname:Muzammil;given-names:Salmansurname:Sweet;given-names:Raymondsurname:Almagro;given-names:Juan Carlossurname:Gilliland;given-names:Gary L.TITLEKEYWORDSfront820160Structural diversity in a human antibody germline library0.9945056speciescleaner02023-06-28T16:03:59ZMESH:humanprotein_typeMESH:cleaner02023-06-28T15:45:47ZantibodyABSTRACTabstract_title_158ABSTRACTABSTRACTabstract67To support antibody therapeutic development, the crystal structures of a set of 16 germline variants composed of 4 different kappa light chains paired with 4 different heavy chains have been determined. All four heavy chains of the antigen-binding fragments (Fabs) have the same complementarity-determining region (CDR) H3 that was reported in an earlier Fab structure. The structure analyses include comparisons of the overall structures, canonical structures of the CDRs and the VH:VL packing interactions. The CDR conformations for the most part are tightly clustered, especially for the ones with shorter lengths. The longer CDRs with tandem glycines or serines have more conformational diversity than the others. CDR H3, despite having the same amino acid sequence, exhibits the largest conformational diversity. About half of the structures have CDR H3 conformations similar to that of the parent; the others diverge significantly. One conclusion is that the CDR H3 conformations are influenced by both their amino acid sequence and their structural environment determined by the heavy and light chain pairing. The stem regions of 14 of the variant pairs are in the ‘kinked’ conformation, and only 2 are in the extended conformation. The packing of the VH and VL domains is consistent with our knowledge of antibody structure, and the tilt angles between these domains cover a range of 11 degrees. Two of 16 structures showed particularly large variations in the tilt angles when compared with the other pairings. The structures and their analyses provide a rich foundation for future antibody modeling and engineering efforts.protein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.9971195evidencecleaner02023-06-28T19:10:19ZDUMMY:crystal structuresstructure_elementSO:cleaner02023-06-28T15:55:50Zkappa light chainsstructure_elementSO:cleaner02023-06-28T15:56:14Zheavy chainsstructure_elementSO:cleaner02023-06-28T15:56:24Zheavy chainsstructure_elementSO:cleaner02023-06-28T15:58:19Zantigen-binding fragments0.29639018structure_elementcleaner02023-06-28T15:58:45ZSO:Fabs0.9439749structure_elementcleaner02023-06-29T08:49:24ZSO:complementarity-determining region0.99692184structure_elementcleaner02023-06-28T16:25:21ZSO:CDR0.93109226structure_elementcleaner02023-06-28T19:05:14ZSO:H30.37466994structure_elementcleaner02023-06-28T15:59:03ZSO:Fab0.9929575evidencecleaner02023-06-28T19:10:54ZDUMMY:structure0.553402experimental_methodcleaner02023-06-29T09:05:49ZMESH:structure analyses0.9943771evidencecleaner02023-06-28T19:10:57ZDUMMY:structures0.80576843evidencecleaner02023-06-28T19:11:00ZDUMMY:structures0.99496186structure_elementcleaner02023-06-28T19:04:38ZSO:CDRscomplex_assemblyGO:melaniev@ebi.ac.uk2023-07-21T09:37:10ZVH:VLbond_interactionMESH:melaniev@ebi.ac.uk2023-07-28T14:19:44Zpacking interactions0.97404987structure_elementcleaner02023-06-28T16:25:21ZSO:CDR0.67458373protein_statecleaner02023-06-29T09:26:48ZDUMMY:longer0.9963917structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.95799243residue_namecleaner02023-06-29T09:11:47ZSO:glycines0.98402774residue_namecleaner02023-06-29T09:11:50ZSO:serinesstructure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T19:05:14ZH30.99673104evidencecleaner02023-06-28T19:11:02ZDUMMY:structuresstructure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T19:05:14ZH3structure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T19:05:14ZH3structure_elementSO:cleaner02023-06-28T16:16:30Zheavystructure_elementSO:cleaner02023-06-28T16:13:38Zlight chain0.9969053structure_elementcleaner02023-06-29T08:49:40ZSO:stem regionsprotein_stateDUMMY:cleaner02023-06-29T07:42:04Zkinkedprotein_stateDUMMY:cleaner02023-06-29T07:41:57Zextended0.99790895structure_elementcleaner02023-06-29T08:49:44ZSO:VH0.9977047structure_elementcleaner02023-06-28T19:10:07ZSO:VLprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.9943786evidencecleaner02023-06-28T19:11:05ZDUMMY:structureevidenceDUMMY:cleaner02023-06-28T16:02:50Ztilt angles0.9967739evidencecleaner02023-06-28T19:11:08ZDUMMY:structuresevidenceDUMMY:cleaner02023-06-28T16:02:51Ztilt angles0.9965509evidencecleaner02023-06-28T19:11:13ZDUMMY:structuresprotein_typeMESH:cleaner02023-06-28T15:45:48ZantibodyINTROtitle_11705IntroductionINTROparagraph1718At present, therapeutic antibodies are the largest class of biotherapeutic proteins that are in clinical trials. The use of monoclonal antibodies as therapeutics began in the early 1980s, and their composition has transitioned from murine antibodies to generally less immunogenic humanized and human antibodies. The technologies currently used to obtain human antibodies include transgenic mice containing human antibody repertoires, cloning directly from human B cells, and in vitro selection from antibody libraries using various display technologies. Once a candidate antibody is identified, protein engineering is usually required to produce a molecule with the right biophysical and functional properties. All engineering efforts are guided by our understanding of the atomic structures of antibodies. In such efforts, the crystal structure of the specific antibody may not be available, but modeling can be used to guide the engineering efforts. Today's antibody modeling approaches, which normally focus on the variable region, are being developed by the application of structural principles and insights that are evolving as our knowledge of antibody structures continues to expand.protein_typeMESH:cleaner02023-06-28T16:03:37Zantibodiesprotein_typeMESH:cleaner02023-06-28T16:03:38Zantibodies0.37784785taxonomy_domaincleaner02023-06-28T16:06:23ZDUMMY:murineprotein_typeMESH:cleaner02023-06-28T16:03:38Zantibodies0.52457434speciescleaner02023-06-28T16:03:59ZMESH:humanprotein_typeMESH:cleaner02023-06-28T16:03:38Zantibodies0.85534525speciescleaner02023-06-28T16:03:58ZMESH:humanprotein_typeMESH:cleaner02023-06-28T16:03:38Zantibodies0.36052313taxonomy_domaincleaner02023-06-28T16:07:17ZDUMMY:mice0.7995312speciescleaner02023-06-28T16:03:59ZMESH:humanprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.9800163speciescleaner02023-06-28T16:03:59ZMESH:humanexperimental_methodMESH:cleaner02023-06-28T16:07:56Zin vitro selection0.68230623experimental_methodcleaner02023-06-29T09:06:00ZMESH:antibody librariesprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibodyexperimental_methodMESH:cleaner02023-06-28T16:08:31Zprotein engineering0.96321344evidencecleaner02023-06-28T16:08:57ZDUMMY:atomic structuresprotein_typeMESH:cleaner02023-06-28T16:03:38Zantibodies0.99743086evidencecleaner02023-06-28T16:09:05ZDUMMY:crystal structureprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibodyprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.9954084structure_elementcleaner02023-06-28T16:09:22ZSO:variable regionprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.9851259evidencecleaner02023-06-28T19:11:18ZDUMMY:structuresINTROparagraph2909Our current structural knowledge of antibodies is based on a multitude of studies that used many techniques to gain insight into the functional and structural properties of this class of macromolecule. Five different antibody isotypes occur, IgG, IgD, IgE, IgA and IgM, and each isotype has a unique role in the adaptive immune system. IgG, IgD and IgE isotypes are composed of 2 heavy chains (HCs) and 2 light chains (LCs) linked through disulfide bonds, while IgA and IgM are double and quintuple versions of antibodies, respectively. Isotypes IgG, IgD and IgA each have 4 domains, one variable (V) and 3 constant (C) domains, while IgE and IgM each have the same 4 domains along with an additional C domain. These multimeric forms are linked with an additional J chain. The LCs that associate with the HCs are divided into 2 functionally indistinguishable classes, κ and λ. Both κ and λ polypeptide chains are composed of a single V domain and a single C domain.protein_typeMESH:cleaner02023-06-28T16:03:38Zantibodiesprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.5808187proteincleaner02023-06-28T16:10:07ZPR:IgG0.579378proteincleaner02023-06-28T16:10:20ZPR:IgD0.4901906proteincleaner02023-06-28T16:10:34ZPR:IgE0.48674142proteincleaner02023-06-28T16:10:53ZPR:IgA0.5469069proteincleaner02023-06-28T16:11:01ZPR:IgM0.4615833proteincleaner02023-06-28T16:10:08ZPR:IgG0.5355316proteincleaner02023-06-28T16:10:46ZPR:IgD0.45904422proteincleaner02023-06-28T16:10:35ZPR:IgE0.91602886structure_elementcleaner02023-06-28T15:56:24ZSO:heavy chains0.99714833structure_elementcleaner02023-06-28T16:11:31ZSO:HCs0.8479442structure_elementcleaner02023-06-28T16:14:07ZSO:light chains0.99629045structure_elementcleaner02023-06-28T16:11:38ZSO:LCsptmMESH:melaniev@ebi.ac.uk2023-07-06T15:17:35Zdisulfide bonds0.58396065proteincleaner02023-06-28T16:10:55ZPR:IgA0.6218557proteincleaner02023-06-28T16:11:02ZPR:IgMprotein_typeMESH:cleaner02023-06-28T16:03:38Zantibodies0.5306497proteincleaner02023-06-28T16:10:08ZPR:IgG0.51756364proteincleaner02023-06-28T16:10:47ZPR:IgD0.6528544proteincleaner02023-06-28T16:10:55ZPR:IgA0.99655485structure_elementcleaner02023-06-29T08:49:51ZSO:variable0.9962674structure_elementcleaner02023-06-28T16:12:17ZSO:V0.995513structure_elementcleaner02023-06-29T08:49:58ZSO:constant0.9924636structure_elementcleaner02023-06-28T16:12:08ZSO:C0.6833741proteincleaner02023-06-28T16:10:35ZPR:IgE0.7602459proteincleaner02023-06-28T16:11:02ZPR:IgMstructure_elementSO:cleaner02023-06-29T08:13:46ZC domain0.9844195structure_elementcleaner02023-06-29T08:50:04ZSO:J0.99804425structure_elementcleaner02023-06-28T16:11:39ZSO:LCs0.9981816structure_elementcleaner02023-06-28T16:11:32ZSO:HCs0.91732925structure_elementcleaner02023-06-28T16:15:20ZSO:κ0.809085structure_elementcleaner02023-06-28T16:15:35ZSO:λ0.923577structure_elementcleaner02023-06-28T16:15:21ZSO:κ0.8177861structure_elementcleaner02023-06-28T16:15:36ZSO:λstructure_elementSO:melaniev@ebi.ac.uk2023-07-06T15:14:51ZV domainstructure_elementSO:cleaner02023-06-29T08:13:25ZC domainINTROparagraph3893The heavy and light chains are composed of structural domains that have ∼110 amino acid residues. These domains have a common folding pattern often referred to as the “immunoglobulin fold,” formed by the packing together of 2 anti-parallel β-sheets. All immunoglobulin chains have an N-terminal V domain followed by 1 to 4 C domains, depending upon the chain type. In antibodies, the heavy and light chain V domains pack together forming the antigen combining site. This site, which interacts with the antigen (or target), is the focus of current antibody modeling efforts. This interaction site is composed of 6 complementarity-determining regions (CDRs) that were identified in early antibody amino acid sequence analyses to be hypervariable in nature, and thus are responsible for the sequence and structural diversity of our antibody repertoire.0.28037655structure_elementcleaner02023-06-29T08:50:09ZSO:heavystructure_elementSO:cleaner02023-06-28T16:14:08Zlight chains0.8056854structure_elementcleaner02023-06-29T08:50:22ZSO:structural domainsresidue_rangeDUMMY:cleaner02023-06-29T09:14:08Z∼110 amino acid residues0.9660743structure_elementcleaner02023-06-29T08:50:29ZSO:immunoglobulin fold0.98161864structure_elementcleaner02023-06-29T08:50:36ZSO:anti-parallel β-sheets0.9119513protein_typecleaner02023-06-28T19:09:00ZMESH:immunoglobulin chainsstructure_elementSO:melaniev@ebi.ac.uk2023-07-06T15:15:03ZV domainstructure_elementSO:cleaner02023-06-28T16:21:31ZC domains0.5746893protein_typecleaner02023-06-28T16:03:38ZMESH:antibodiesstructure_elementSO:cleaner02023-06-28T20:43:25Zheavy and light chainstructure_elementSO:cleaner02023-06-28T16:21:51ZV domains0.9981706sitecleaner02023-06-29T09:10:03ZSO:antigen combining site0.774341protein_typecleaner02023-06-28T15:45:48ZMESH:antibody0.99761343sitecleaner02023-06-29T09:10:11ZSO:interaction site0.9165287structure_elementcleaner02023-06-29T08:50:44ZSO:complementarity-determining regions0.99350005structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.92376745experimental_methodcleaner02023-06-29T09:06:06ZMESH:antibody amino acid sequence analyses0.99413955protein_statecleaner02023-06-29T09:26:56ZDUMMY:hypervariableprotein_typeMESH:cleaner02023-06-28T15:45:48ZantibodyINTROparagraph4750The sequence diversity of the CDR regions presents a substantial challenge to antibody modeling. However, an initial structural analysis of the combining sites of the small set of structures of immunoglobulin fragments available in the 1980s found that 5 of the 6 hypervariable loops or CDRs had canonical structures (a limited set of main-chain conformations). A CDR canonical structure is defined by its length and conserved residues located in the hypervariable loop and framework residues (V-region residues that are not part of the CDRs). Furthermore, studies of antibody sequences revealed that the total number of canonical structures are limited for each CDR, indicating possibly that antigen recognition may be affected by structural restrictions at the antigen-binding site. Later studies found that the CDR loop length is the primary determining factor of antigen-binding site topography because it is the primary factor for determining a canonical structure. Additional efforts have led to our current understanding that the LC CDRs L1, L2, and L3 have preferred sets of canonical structures based on length and amino acid sequence composition. This was also found to be the case for the H1 and H2 CDRs. Classification schemes for the canonical structures of these 5 CDRs have emerged and evolved as the number of depositions in the Protein Data Bank of Fab fragments of antibodies grow. Recently, a comprehensive CDR classification scheme was reported identifying 72 clusters of conformations observed in antibody structures. The knowledge and predictability of these CDR canonical structures have greatly advanced antibody modeling efforts.0.9892019structure_elementcleaner02023-06-29T08:50:50ZSO:CDR regionsprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.86392236experimental_methodcleaner02023-06-29T09:06:10ZMESH:structural analysis0.9967412sitecleaner02023-06-29T09:10:17ZSO:combining sites0.99583673evidencecleaner02023-06-28T19:11:25ZDUMMY:structures0.97308254structure_elementcleaner02023-06-29T08:50:55ZSO:hypervariable loops0.9967535structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.79579794structure_elementcleaner02023-06-28T16:25:21ZSO:CDR0.96811986structure_elementcleaner02023-06-29T08:51:00ZSO:hypervariable loop0.79810786structure_elementcleaner02023-06-29T08:51:45ZSO:framework residues0.93935466structure_elementcleaner02023-06-29T08:51:53ZSO:V-region0.99704546structure_elementcleaner02023-06-28T19:04:38ZSO:CDRsprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.9926368structure_elementcleaner02023-06-28T16:25:21ZSO:CDR0.99790645sitecleaner02023-06-29T09:10:21ZSO:antigen-binding site0.954146structure_elementcleaner02023-06-29T08:51:56ZSO:CDR loop0.9959662sitecleaner02023-06-29T09:10:24ZSO:antigen-binding site0.6664975structure_elementcleaner02023-06-28T16:24:54ZSO:LC0.9848006structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.9951461structure_elementcleaner02023-06-28T16:26:29ZSO:L10.9955865structure_elementcleaner02023-06-28T20:09:53ZSO:L20.99560404structure_elementcleaner02023-06-29T08:52:03ZSO:L30.90607065structure_elementcleaner02023-06-28T19:04:09ZSO:H10.6061986structure_elementcleaner02023-06-28T16:26:20ZSO:H20.97096324structure_elementcleaner02023-06-28T19:04:37ZSO:CDRs0.9894225structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.4258705structure_elementcleaner02023-06-28T15:59:04ZSO:Fabprotein_typeMESH:cleaner02023-06-28T16:03:38Zantibodiesstructure_elementSO:cleaner02023-06-28T16:25:21ZCDRprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.99535346evidencecleaner02023-06-28T19:11:30ZDUMMY:structures0.95264316structure_elementcleaner02023-06-28T16:25:19ZSO:CDR0.65532506evidencecleaner02023-06-28T19:11:33ZDUMMY:structuresprotein_typeMESH:cleaner02023-06-28T15:45:48ZantibodyINTROparagraph6405In contrast to CDRs L1, L2, L3, H1 and H2, no canonical structures have been observed for CDR H3, which is the most variable in length and amino acid sequence. Some clustering of conformations was observed for the shortest lengths; however, for the longer loops, only the portions nearest the framework (torso, stem or anchor region) were found to have defined conformations. In the torso region, 2 primary groups could be identified, which led to sequence-based rules that can predict with some degree of reliability the conformation of the stem region. The “kinked” or “bulged” conformation is the most prevalent, but an “extended” or “non-bulged” conformation is also, but less frequently, observed. The cataloging and development of the rules for predicting the conformation of the anchor region of CDR H3 continue to be refined, producing new insight into the CDR H3 conformations and new tools for antibody engineering.0.9609944structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.8862208structure_elementcleaner02023-06-28T16:26:30ZSO:L10.9949463structure_elementcleaner02023-06-28T20:09:53ZSO:L20.99527967structure_elementcleaner02023-06-29T08:52:14ZSO:L30.9794373structure_elementcleaner02023-06-28T19:04:10ZSO:H10.9499178structure_elementcleaner02023-06-28T16:26:21ZSO:H2evidenceDUMMY:cleaner02023-06-28T20:44:12Zstructuresstructure_elementSO:cleaner02023-06-28T19:06:45ZCDRstructure_elementSO:cleaner02023-06-28T19:05:14ZH30.9731328structure_elementcleaner02023-06-29T08:52:18ZSO:loops0.9971501structure_elementcleaner02023-06-29T08:52:25ZSO:framework0.99759847structure_elementcleaner02023-06-29T08:52:29ZSO:torso0.9975243structure_elementcleaner02023-06-29T08:52:32ZSO:stem0.9965924structure_elementcleaner02023-06-29T08:52:35ZSO:anchor region0.99673957structure_elementcleaner02023-06-29T08:52:42ZSO:torso region0.99730873structure_elementcleaner02023-06-29T08:52:49ZSO:stem region0.9651129protein_statecleaner02023-06-29T07:42:05ZDUMMY:kinked0.9021778protein_statecleaner02023-06-29T09:27:20ZDUMMY:bulged0.9788072protein_statecleaner02023-06-29T07:41:57ZDUMMY:extended0.9845164protein_statecleaner02023-06-29T09:27:24ZDUMMY:non-bulged0.9973566structure_elementcleaner02023-06-29T08:52:56ZSO:anchor regionstructure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T19:05:13ZH30.8594353structure_elementcleaner02023-06-28T16:25:21ZSO:CDR0.6657651structure_elementcleaner02023-06-28T19:05:14ZSO:H3protein_typeMESH:cleaner02023-06-28T15:45:48ZantibodyINTROparagraph7347Current antibody modeling approaches take advantage of the most recent advances in homology modeling, the evolving understanding of the CDR canonical structures, the emerging rules for CDR H3 modeling and the growing body of antibody structural data available from the PDB. Recent antibody modeling assessments show continued improvement in the quality of the models being generated by a variety of modeling methods. Although antibody modeling is improving, the latest assessment revealed a number of challenges that need to be overcome to provide accurate 3-dimensional models of antibody V regions, including accuracies in the modeling of CDR H3. The need for improvement in this area was also highlighted in a recent study reporting an approach and results that may influence future antibody modeling efforts. One important finding of the antibody modeling assessments was that errors in the structural templates that are used as the basis for homology models can propagate into the final models, producing inaccuracies that may negatively influence the predictive nature of the V region model.protein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.9942754experimental_methodcleaner02023-06-28T19:08:24ZMESH:homology modelingstructure_elementSO:cleaner02023-06-28T16:25:21ZCDR0.46883506evidencecleaner02023-06-28T19:11:39ZDUMMY:structuresstructure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T19:05:14ZH3protein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.76543283experimental_methodcleaner02023-06-28T19:06:07ZMESH:antibody modeling assessmentsprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibodyprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.9220402structure_elementcleaner02023-06-28T19:05:50ZSO:V regionsstructure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T19:05:14ZH3protein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.8185734experimental_methodcleaner02023-06-28T19:06:09ZMESH:antibody modeling assessments0.99114794experimental_methodcleaner02023-06-29T09:06:15ZMESH:homology models0.94426894structure_elementcleaner02023-06-29T08:53:04ZSO:V regionINTROparagraph8445To support antibody engineering and therapeutic development efforts, a phage library was designed and constructed based on a limited number of scaffolds built with frequently used human germ-line IGV and IGJ gene segments that encode antigen combining sites suitable for recognition of peptides and proteins. This Fab library is composed of 3 HC germlines, IGHV1-69 (H1-69), IGHV3-23 (H3-23) and IGHV5-51(H5-51), and 4 LC germlines (all κ), IGKV1-39 (L1-39), IGKV3-11 (L3-11), IGKV3-20 (L3-20) and IGKV4-1 (L4-1). Selection of these genes was based on the high frequency of their use and their cognate canonical structures that were found binding to peptides and proteins, as well as their ability to be expressed in bacteria and displayed on filamentous phage. The implementation of the library involves the diversification of the human germline genes to mimic that found in natural human libraries.protein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.8285667experimental_methodcleaner02023-06-29T09:06:19ZMESH:phage library0.99304044speciescleaner02023-06-28T16:03:59ZMESH:human0.5477985structure_elementcleaner02023-06-29T09:37:42ZSO:IGV0.37411508structure_elementcleaner02023-06-29T09:37:44ZSO:IGJ0.9979419sitecleaner02023-06-29T09:10:29ZSO:antigen combining sites0.22796038structure_elementcleaner02023-06-28T15:59:04ZSO:Fab0.3749512structure_elementcleaner02023-06-29T08:55:32ZSO:HCmutantMESH:cleaner02023-06-28T21:02:43ZIGHV1-69mutantMESH:cleaner02023-06-28T21:03:12ZH1-69mutantMESH:cleaner02023-06-28T21:03:44ZIGHV3-23mutantMESH:cleaner02023-06-28T21:04:11ZH3-23mutantMESH:cleaner02023-06-28T21:04:40ZIGHV5-51mutantMESH:cleaner02023-06-28T21:05:04ZH5-510.29352522structure_elementcleaner02023-06-28T16:24:55ZSO:LC0.68899107structure_elementcleaner02023-06-28T16:15:21ZSO:κmutantMESH:cleaner02023-06-28T21:05:26ZIGKV1-39mutantMESH:cleaner02023-06-28T21:05:49ZL1-39mutantMESH:cleaner02023-06-28T21:06:12ZIGKV3-11mutantMESH:cleaner02023-06-28T21:06:33ZL3-11mutantMESH:cleaner02023-06-28T21:06:55ZIGKV3-20mutantMESH:cleaner02023-06-28T21:07:14ZL3-20mutantMESH:cleaner02023-06-28T21:07:38ZIGKV4-1mutantMESH:cleaner02023-06-28T21:07:59ZL4-1evidenceDUMMY:cleaner02023-06-28T20:53:28Zstructuresexperimental_methodMESH:cleaner02023-06-28T21:02:06Zexpressed in bacteriaexperimental_methodMESH:cleaner02023-06-28T21:02:13Zdisplayed on filamentous phage0.9926621speciescleaner02023-06-28T16:03:59ZMESH:human0.9930802speciescleaner02023-06-28T16:03:59ZMESH:humanINTROparagraph9350The crystal structure determinations and structural analyses of all germline Fabs in the library described above along with the structures of a fourth HC germline, IGHV3-53 (H3-53), paired with the 4 LCs of the library have been carried out to support antibody therapeutic development. All 16 HCs of the Fabs have the same CDR H3 that was reported in an earlier Fab structure. This is the first systematic study of the same VH and VL structures in the context of different pairings. The structure analyses include comparisons of the overall structures, canonical structures of the L1, L2, L3, H1 and H2 CDRs, the structures of all CDR H3s, and the VH:VL packing interactions. The structures and their analyses provide a foundation for future antibody engineering and structure determination efforts.0.9365645experimental_methodcleaner02023-06-29T09:06:25ZMESH:crystal structure determinations0.9690031experimental_methodcleaner02023-06-29T09:06:28ZMESH:structural analyses0.18112527structure_elementcleaner02023-06-28T15:58:46ZSO:Fabs0.99385357evidencecleaner02023-06-28T19:11:45ZDUMMY:structures0.4604112structure_elementcleaner02023-06-29T08:55:41ZSO:HC0.9870631mutantcleaner02023-06-29T09:25:45ZMESH:IGHV3-53mutantMESH:cleaner02023-06-28T21:08:31ZH3-530.99305177structure_elementcleaner02023-06-28T16:11:39ZSO:LCsprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.9857884structure_elementcleaner02023-06-28T16:11:32ZSO:HCs0.23558842structure_elementcleaner02023-06-28T15:58:46ZSO:Fabsstructure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T19:05:14ZH30.24147022structure_elementcleaner02023-06-28T15:59:04ZSO:Fab0.9950139evidencecleaner02023-06-28T19:11:56ZDUMMY:structure0.9822842structure_elementcleaner02023-06-29T08:55:44ZSO:VH0.956339structure_elementcleaner02023-06-28T19:10:07ZSO:VL0.9961636evidencecleaner02023-06-28T19:11:59ZDUMMY:structures0.99412364evidencecleaner02023-06-28T19:12:02ZDUMMY:structures0.97373337evidencecleaner02023-06-28T19:12:04ZDUMMY:structures0.9947988structure_elementcleaner02023-06-28T16:26:30ZSO:L10.9907031structure_elementcleaner02023-06-28T20:09:53ZSO:L20.9922057structure_elementcleaner02023-06-29T08:55:50ZSO:L30.9905301structure_elementcleaner02023-06-28T19:04:10ZSO:H10.9891143structure_elementcleaner02023-06-28T16:26:21ZSO:H20.9166478structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.98723364evidencecleaner02023-06-28T19:12:07ZDUMMY:structuresstructure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T19:07:47ZH3scomplex_assemblyGO:melaniev@ebi.ac.uk2023-07-21T09:37:57ZVH:VLbond_interactionMESH:melaniev@ebi.ac.uk2023-07-28T14:19:44Zpacking interactions0.98796034evidencecleaner02023-06-28T19:12:10ZDUMMY:structuresprotein_typeMESH:cleaner02023-06-28T15:45:48ZantibodyRESULTStitle_110150ResultsRESULTStitle_210158Crystal structures0.9969361evidencecleaner02023-06-28T19:12:15ZDUMMY:Crystal structuresd37e422.xmlt0001TABLEtable_caption10177Crystal data, X-ray data, and refinement statistics.0.99486244evidencecleaner02023-06-28T19:12:18ZDUMMY:Crystal data0.99513537evidencecleaner02023-06-28T19:12:21ZDUMMY:X-ray dataevidenceDUMMY:cleaner02023-06-28T19:12:33Zrefinement statisticsd37e422.xmlt0001TABLEtable<?xml version="1.0" encoding="UTF-8"?>
<table frame="hsides" rules="groups"><colgroup><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/></colgroup><thead><tr><th align="left">Fab<hr/></th><th align="center">H1-69:L1-39<hr/></th><th align="center">H1-69:L3-11<hr/></th><th align="center">H1-69:L3-20<hr/></th><th align="center">H1-69:L4-1<hr/></th></tr><tr><th align="left"><italic>PDB identifier</italic></th><th align="center">5I15</th><th align="center">5I16</th><th align="center">5I17</th><th align="center">5I18</th></tr></thead><tbody><tr><td align="left">Crystal Data</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left">Crystallization Solution</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Buffer, pH</td><td align="center">0.1 M MES- pH 6.5</td><td align="center">0.1 M MES pH 6.5</td><td align="center">0.1 M MES, pH 6.5</td><td align="center">0.1 M HEPES, pH 7.5</td></tr><tr><td align="left"> Precipitant<xref rid="t1fn0001" ref-type="fn"><sup>1</sup></xref></td><td align="center">5 M Na Formate</td><td align="center">25% PEG 3350</td><td align="center">2.0 M Amm Sulfate</td><td align="center">10% PEG 8000</td></tr><tr><td align="left"> Additive<xref rid="t1fn0001" ref-type="fn"><sup>1</sup></xref></td><td align="left"> </td><td align="center">0.2 M Na Formate</td><td align="center">5% MPD</td><td align="center">8% EG</td></tr><tr><td align="left"> Space Group</td><td align="center">P3<sub>1</sub>21</td><td align="center">C2</td><td align="center">P422</td><td align="center">P42<sub>1</sub>2</td></tr><tr><td align="left"> Molecules/AU</td><td align="center">1</td><td align="center">2</td><td align="center">2</td><td align="center">1</td></tr><tr><td align="left"> Unit Cell</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> a(Å)</td><td align="center">129.2</td><td align="center">212.0</td><td align="center">152.5</td><td align="center">120.0</td></tr><tr><td align="left"> b(Å)</td><td align="center">129.2</td><td align="center">55.1</td><td align="center">152.5</td><td align="center">120.0</td></tr><tr><td align="left"> c(Å)</td><td align="center">91.8</td><td align="center">80.3</td><td align="center">123.4</td><td align="center">64.2</td></tr><tr><td align="left"> β(°)</td><td align="center">90.0</td><td align="center">97.8</td><td align="center">90.0</td><td align="center">90.0</td></tr><tr><td align="left"> γ(°)</td><td align="center">120.0</td><td align="center">90.0</td><td align="center">90.0</td><td align="center">90.0</td></tr><tr><td align="left"> V<sub>m</sub> (Å<sup>3</sup>/Da)</td><td align="center">4.67</td><td align="center">2.44</td><td align="center">3.77</td><td align="center">2.39</td></tr><tr><td align="left"> Solvent Content (%)</td><td align="center">74</td><td align="center">50</td><td align="center">67</td><td align="center">48</td></tr><tr><td align="left">X-Ray Data<xref rid="t1fn0002" ref-type="fn"><sup>2</sup></xref></td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Resolution (Å)</td><td align="center">30-2.6 (2.7-2.6)</td><td align="center">30.0-1.9 (1.95-1.9)</td><td align="center">30.0-3.3 (3.4-3.3)</td><td align="center">30-1.9 (2.0-1.9)</td></tr><tr><td align="left"> Measured Reflections</td><td align="center">136,745 (8,650)</td><td align="center">241,145 (16,580)</td><td align="center">237,504 (15,007)</td><td align="center">801,080 (19,309)</td></tr><tr><td align="left"> Unique Reflections</td><td align="center">27,349 (1,730)</td><td align="center">71,932 (5,198)</td><td align="center">22,379 (1,590)</td><td align="center">35,965 (2,194)</td></tr><tr><td align="left"> Completeness (%)</td><td align="center">99.3 (98.7)</td><td align="center">99.0 (97.3)</td><td align="center">99.5 (96.8)</td><td align="center">98.5 (82.8)</td></tr><tr><td align="left"> Redundancy</td><td align="center">5.0 (5.0)</td><td align="center">3.4 (3.2)</td><td align="center">10.6 (9.4)</td><td align="center">22.3 (8.8)</td></tr><tr><td align="left"> R<sub>merge</sub></td><td align="center">0.048 (0.522)</td><td align="center">0.044 (0.245)</td><td align="center">0.086 (0.536)</td><td align="center">0.093 (0.231)</td></tr><tr><td align="left"> < I/σ ></td><td align="center">21.2 (3.9)</td><td align="center">17.8 (4.7)</td><td align="center">25.5 (4.5)</td><td align="center">29.2 (8.1)</td></tr><tr><td align="left"> B-factor (Å<sup>2</sup>)</td><td align="center">60.5</td><td align="center">33.2</td><td align="center">61.0</td><td align="center">19.6</td></tr><tr><td align="left">Refinement</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Resolution (Å)</td><td align="center">15-2.6</td><td align="center">15-1.9</td><td align="center">15-3.3</td><td align="center">15-1.9</td></tr><tr><td align="left"> Number of Reflections</td><td align="center">26,238</td><td align="center">70,346</td><td align="center">21,197</td><td align="center">34,850</td></tr><tr><td align="left"> Number of All Atoms</td><td align="center">3,224</td><td align="center">6,975</td><td align="center">6,398</td><td align="center">3,695</td></tr><tr><td align="left"> Number of Waters</td><td align="center">2</td><td align="center">472</td><td align="center">0</td><td align="center">399</td></tr><tr><td align="left"> R-factor (%)</td><td align="center">20.5</td><td align="center">19.2</td><td align="center">20.2</td><td align="center">16.7</td></tr><tr><td align="left"> R-free (%)</td><td align="center">24.1</td><td align="center">22.2</td><td align="center">24.7</td><td align="center">21.3</td></tr><tr><td align="left">RMSD</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Bond Lengths (Å)</td><td align="center">0.006</td><td align="center">0.005</td><td align="center">0.005</td><td align="center">0.008</td></tr><tr><td align="left"> Bond Angles (°)</td><td align="center">1.2</td><td align="center">1.1</td><td align="center">1.0</td><td align="center">1.1</td></tr><tr><td align="left"> Mean B-factor (Å<sup>2</sup>)</td><td align="center">65.3</td><td align="center">34.4</td><td align="center">80.1</td><td align="center">20.0</td></tr><tr><td align="left">Ramachandran Plot (%)</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Outliers</td><td align="center">0.0</td><td align="center">0.0</td><td align="center">0.9</td><td align="center">0.0</td></tr><tr><td align="left"> Favored</td><td align="center">92.3</td><td align="center">96.9</td><td align="center">93.1</td><td align="center">96.9</td></tr></tbody></table>
10230Fab H1-69:L1-39 H1-69:L3-11 H1-69:L3-20 H1-69:L4-1 PDB identifier 5I15 5I16 5I17 5I18 Crystal Data Crystallization Solution Buffer, pH 0.1 M MES- pH 6.5 0.1 M MES pH 6.5 0.1 M MES, pH 6.5 0.1 M HEPES, pH 7.5 Precipitant1 5 M Na Formate 25% PEG 3350 2.0 M Amm Sulfate 10% PEG 8000 Additive1 0.2 M Na Formate 5% MPD 8% EG Space Group P3121 C2 P422 P4212 Molecules/AU 1 2 2 1 Unit Cell a(Å) 129.2 212.0 152.5 120.0 b(Å) 129.2 55.1 152.5 120.0 c(Å) 91.8 80.3 123.4 64.2 β(°) 90.0 97.8 90.0 90.0 γ(°) 120.0 90.0 90.0 90.0 Vm (Å3/Da) 4.67 2.44 3.77 2.39 Solvent Content (%) 74 50 67 48 X-Ray Data2 Resolution (Å) 30-2.6 (2.7-2.6) 30.0-1.9 (1.95-1.9) 30.0-3.3 (3.4-3.3) 30-1.9 (2.0-1.9) Measured Reflections 136,745 (8,650) 241,145 (16,580) 237,504 (15,007) 801,080 (19,309) Unique Reflections 27,349 (1,730) 71,932 (5,198) 22,379 (1,590) 35,965 (2,194) Completeness (%) 99.3 (98.7) 99.0 (97.3) 99.5 (96.8) 98.5 (82.8) Redundancy 5.0 (5.0) 3.4 (3.2) 10.6 (9.4) 22.3 (8.8) Rmerge 0.048 (0.522) 0.044 (0.245) 0.086 (0.536) 0.093 (0.231) < I/σ > 21.2 (3.9) 17.8 (4.7) 25.5 (4.5) 29.2 (8.1) B-factor (Å2) 60.5 33.2 61.0 19.6 Refinement Resolution (Å) 15-2.6 15-1.9 15-3.3 15-1.9 Number of Reflections 26,238 70,346 21,197 34,850 Number of All Atoms 3,224 6,975 6,398 3,695 Number of Waters 2 472 0 399 R-factor (%) 20.5 19.2 20.2 16.7 R-free (%) 24.1 22.2 24.7 21.3 RMSD Bond Lengths (Å) 0.006 0.005 0.005 0.008 Bond Angles (°) 1.2 1.1 1.0 1.1 Mean B-factor (Å2) 65.3 34.4 80.1 20.0 Ramachandran Plot (%) Outliers 0.0 0.0 0.9 0.0 Favored 92.3 96.9 93.1 96.9 d37e422.xmlt0001TABLEtable_footnote12072Abbreviations: Amm, ammonium;EG, ethylene glycol; PEG, polyethylene glycol.d37e422.xmlt0001TABLEtable_footnote12148Values for high-resolution shell are in parentheses.d37e892.xmlt0001TABLEtable_caption12201(Continued) Crystal data, X-ray data, and refinement statistics.0.9933119evidencecleaner02023-06-28T19:14:53ZDUMMY:Crystal data0.98985547evidencecleaner02023-06-28T19:14:56ZDUMMY:X-ray dataevidenceDUMMY:cleaner02023-06-28T19:15:10Zrefinement statisticsd37e892.xmlt0001TABLEtable<?xml version="1.0" encoding="UTF-8"?>
<table frame="hsides" rules="groups"><colgroup><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/></colgroup><thead><tr><th align="left">Fab<hr/></th><th align="center">H3-23:L1-39<hr/></th><th align="center">H3-23:L3-11<hr/></th><th align="center">H3-23:L3-20<hr/></th><th align="center">H3-23:L4-1<hr/></th></tr><tr><th align="left"><italic> PDB identifier</italic></th><th align="center">5I19</th><th align="center">5I1A</th><th align="center">5I1C</th><th align="center">5I1D</th></tr></thead><tbody><tr><td align="left">Crystal Data</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left">Crystallization Solution</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Buffer, pH</td><td align="center">No Buffer</td><td align="center">0.1 M Na Acetate, pH 4.5</td><td align="center">0.1 M MES, pH 6.5</td><td align="center">0.1 M HEPES, pH 7.5</td></tr><tr><td align="left"> Precipitant<xref rid="t1fn0003" ref-type="fn"><sup>1</sup></xref></td><td align="center">20% PEG 3350</td><td align="center">2.0 M Amm Sulfate</td><td align="center">16% PEG 3350</td><td align="center">2.0 M Amm Sulfate</td></tr><tr><td align="left"> Additive<xref rid="t1fn0003" ref-type="fn"><sup>1</sup></xref></td><td align="center">0.2 M Li Citrate</td><td align="center">5% PEG 400</td><td align="center">0.2 M Amm Acetate</td><td align="center">2% PEG 400</td></tr><tr><td align="left"> Space Group</td><td align="center">P4<sub>1</sub>2<sub>1</sub>2</td><td align="center">P2<sub>1</sub>2<sub>1</sub>2<sub>1</sub></td><td align="center">P6<sub>2</sub>22</td><td align="center">P2<sub>1</sub>2<sub>1</sub>2<sub>1</sub></td></tr><tr><td align="left"> Molecules/AU</td><td align="center">1</td><td align="center">2</td><td align="center">1</td><td align="center">2</td></tr><tr><td align="left">Unit Cell</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> a(Å)</td><td align="center">96.6</td><td align="center">60.9</td><td align="center">121.5</td><td align="center">62.7</td></tr><tr><td align="left"> b(Å)</td><td align="center">96.6</td><td align="center">110.6</td><td align="center">121.5</td><td align="center">111.0</td></tr><tr><td align="left"> c(Å)</td><td align="center">105.4</td><td align="center">158.9</td><td align="center">160.4</td><td align="center">160.0</td></tr><tr><td align="left"> β(°)</td><td align="center">90</td><td align="center">90</td><td align="center">90</td><td align="center">90</td></tr><tr><td align="left"> V<sub>m</sub> (Å<sup>3</sup>/Da)</td><td align="center">2.60</td><td align="center">2.82</td><td align="center">3.60</td><td align="center">2.90</td></tr><tr><td align="left"> Solvent Content (%)</td><td align="center">53</td><td align="center">56</td><td align="center">66</td><td align="center">57</td></tr><tr><td align="left">X-Ray Data<xref rid="t1fn0004" ref-type="fn"><sup>2</sup></xref></td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Resolution (Å)</td><td align="center">30-2.8 (2.9-2.8)</td><td align="center">30-2.0 (2.1-2.0)</td><td align="center">30-2.25 (2.3-2.25)</td><td align="center">30-2.0 (2.1-2.0)</td></tr><tr><td align="left"> Measured Reflections</td><td align="center">177,681 (12,072)</td><td align="center">351,312 (8,634)</td><td align="center">887,349 (59,919)</td><td align="center">873,523 (49,118)</td></tr><tr><td align="left"> Unique Reflections</td><td align="center">12,678 (899)</td><td align="center">58,989 (2,870)</td><td align="center">32,572 (2,300)</td><td align="center">75,540 (5,343)</td></tr><tr><td align="left"> Completeness (%)</td><td align="center">99.5 (97.4)</td><td align="center">80.9 (54.2)</td><td align="center">96.9 (94.8)</td><td align="center">99.7 (96.9)</td></tr><tr><td align="left"> Redundancy</td><td align="center">14.0 (13.4)</td><td align="center">6.0 (3.0)</td><td align="center">27.2 (26.1)</td><td align="center">11.6 (9.2)</td></tr><tr><td align="left"> R<sub>merge</sub></td><td align="center">0.091 (0.594)</td><td align="center">0.066 (0.204)</td><td align="center">0.086 (0.478)</td><td align="center">0.094 (0.488)</td></tr><tr><td align="left"> < I/σ ></td><td align="center">31.2 (5.1)</td><td align="center">20.4 (4.6)</td><td align="center">37.0 (10.4)</td><td align="center">21.6 (5.0)</td></tr><tr><td align="left"> B-factor (Å<sup>2</sup>)</td><td align="center">42.8</td><td align="center">27.1</td><td align="center">33.7</td><td align="center">29.4</td></tr><tr><td align="left">Refinement</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Resolution (Å)</td><td align="center">15-2.8</td><td align="center">15-2.0</td><td align="center">15-2.25</td><td align="center">15-2.0</td></tr><tr><td align="left"> Number of Reflections</td><td align="center">11,972</td><td align="center">57,599</td><td align="center">31,411</td><td align="center">74,238</td></tr><tr><td align="left"> Number of All Atoms</td><td align="center">3,234</td><td align="center">6,948</td><td align="center">3,472</td><td align="center">7,210</td></tr><tr><td align="left"> Number of Waters</td><td align="center">0</td><td align="center">416</td><td align="center">222</td><td align="center">635</td></tr><tr><td align="left"> R-factor (%)</td><td align="center">23.9</td><td align="center">20.5</td><td align="center">22.0</td><td align="center">21.6</td></tr><tr><td align="left"> R-free (%)</td><td align="center">31.5</td><td align="center">25.5</td><td align="center">26.6</td><td align="center">25.1</td></tr><tr><td align="left">RMSD</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Bond Lengths (Å)</td><td align="center">0.009</td><td align="center">0.010</td><td align="center">0.005</td><td align="center">0.008</td></tr><tr><td align="left"> Bond Angles (°)</td><td align="center">1.3</td><td align="center">1.3</td><td align="center">1.0</td><td align="center">1.1</td></tr><tr><td align="left"> Mean B-factor (Å<sup>2</sup>)</td><td align="center">48.4</td><td align="center">36.7</td><td align="center">47.7</td><td align="center">46.4</td></tr><tr><td align="left">Ramachandran Plot (%)</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Outliers</td><td align="center">0.0</td><td align="center">0.0</td><td align="center">0.0</td><td align="center">0.0</td></tr><tr><td align="left"> Favored</td><td align="center">92.3</td><td align="center">96.8</td><td align="center">97.5</td><td align="center">97.6</td></tr></tbody></table>
12266Fab H3-23:L1-39 H3-23:L3-11 H3-23:L3-20 H3-23:L4-1 PDB identifier 5I19 5I1A 5I1C 5I1D Crystal Data Crystallization Solution Buffer, pH No Buffer 0.1 M Na Acetate, pH 4.5 0.1 M MES, pH 6.5 0.1 M HEPES, pH 7.5 Precipitant1 20% PEG 3350 2.0 M Amm Sulfate 16% PEG 3350 2.0 M Amm Sulfate Additive1 0.2 M Li Citrate 5% PEG 400 0.2 M Amm Acetate 2% PEG 400 Space Group P41212 P212121 P6222 P212121 Molecules/AU 1 2 1 2 Unit Cell a(Å) 96.6 60.9 121.5 62.7 b(Å) 96.6 110.6 121.5 111.0 c(Å) 105.4 158.9 160.4 160.0 β(°) 90 90 90 90 Vm (Å3/Da) 2.60 2.82 3.60 2.90 Solvent Content (%) 53 56 66 57 X-Ray Data2 Resolution (Å) 30-2.8 (2.9-2.8) 30-2.0 (2.1-2.0) 30-2.25 (2.3-2.25) 30-2.0 (2.1-2.0) Measured Reflections 177,681 (12,072) 351,312 (8,634) 887,349 (59,919) 873,523 (49,118) Unique Reflections 12,678 (899) 58,989 (2,870) 32,572 (2,300) 75,540 (5,343) Completeness (%) 99.5 (97.4) 80.9 (54.2) 96.9 (94.8) 99.7 (96.9) Redundancy 14.0 (13.4) 6.0 (3.0) 27.2 (26.1) 11.6 (9.2) Rmerge 0.091 (0.594) 0.066 (0.204) 0.086 (0.478) 0.094 (0.488) < I/σ > 31.2 (5.1) 20.4 (4.6) 37.0 (10.4) 21.6 (5.0) B-factor (Å2) 42.8 27.1 33.7 29.4 Refinement Resolution (Å) 15-2.8 15-2.0 15-2.25 15-2.0 Number of Reflections 11,972 57,599 31,411 74,238 Number of All Atoms 3,234 6,948 3,472 7,210 Number of Waters 0 416 222 635 R-factor (%) 23.9 20.5 22.0 21.6 R-free (%) 31.5 25.5 26.6 25.1 RMSD Bond Lengths (Å) 0.009 0.010 0.005 0.008 Bond Angles (°) 1.3 1.3 1.0 1.1 Mean B-factor (Å2) 48.4 36.7 47.7 46.4 Ramachandran Plot (%) Outliers 0.0 0.0 0.0 0.0 Favored 92.3 96.8 97.5 97.6 d37e892.xmlt0001TABLEtable_footnote14105Abbreviations: Amm, ammonium; PEG, polyethylene glycol.d37e892.xmlt0001TABLEtable_footnote14161Values for high-resolution shell are in parentheses.d37e1371.xmlt0001TABLEtable_caption14214(Continued) Crystal data, X-ray data, and refinement statistics.0.9933119evidencecleaner02023-06-28T19:16:41ZDUMMY:Crystal data0.98985547evidencecleaner02023-06-28T19:16:44ZDUMMY:X-ray dataevidenceDUMMY:cleaner02023-06-28T19:15:41Zrefinement statisticsd37e1371.xmlt0001TABLEtable<?xml version="1.0" encoding="UTF-8"?>
<table frame="hsides" rules="groups"><colgroup><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/></colgroup><thead><tr><th align="left">Fab</th><th align="center">H3-53:L1-39</th><th align="center">H3-53:L3-11</th><th align="center">H3-53:L3-20</th><th align="center">H3-53:L4-1</th></tr></thead><tbody><tr><td align="left"><italic>PDB indentifier</italic></td><td align="center">5I1E</td><td align="center">5I1G</td><td align="center">5I1H</td><td align="center">5I1I</td></tr><tr><td align="left">Crystal Data</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left">Crystallization Solution</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Buffer, pH</td><td align="center">No buffer</td><td align="center">0.1 M Na Acetate pH 4.5</td><td align="center">0.1 M Na Acetate pH 4.5</td><td align="center">0.1M MES, pH 6.5</td></tr><tr><td align="left"> Precipitant<xref rid="t1fn0005" ref-type="fn"><sup>1</sup></xref></td><td align="center">16% PEG 3350</td><td align="center">25% PEG 3350</td><td align="center">19% PEG 4000</td><td align="center">17% PEG 3350</td></tr><tr><td align="left"> Additive<xref rid="t1fn0005" ref-type="fn"><sup>1</sup></xref></td><td align="center">0.2 M Amm Sulfate 5% Dioxane</td><td align="center">0.2 M Li<sub>2</sub>SO<sub>4</sub></td><td align="center">0.2 M Amm Sulfate</td><td align="center">0.2 M Na Formate, 5% MPD</td></tr><tr><td align="left"> Space Group</td><td align="center">P6<sub>5</sub>22</td><td align="center">P6<sub>5</sub>22</td><td align="center">P6<sub>5</sub>22</td><td align="center">P3<sub>1</sub></td></tr><tr><td align="left"> Molecules/AU</td><td align="center">1</td><td align="center">1</td><td align="center">1</td><td align="center">1</td></tr><tr><td align="left">Unit Cell</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> a(Å)</td><td align="center">89.4</td><td align="center">88.1</td><td align="center">89.4</td><td align="center">68.1</td></tr><tr><td align="left"> b(Å)</td><td align="center">89.4</td><td align="center">88.1</td><td align="center">89.4</td><td align="center">68.1</td></tr><tr><td align="left"> c(Å)</td><td align="center">212.4</td><td align="center">219.6</td><td align="center">211.7</td><td align="center">95.6</td></tr><tr><td align="left"> β(°)</td><td align="center">90</td><td align="center">90</td><td align="center">90</td><td align="center">90</td></tr><tr><td align="left"> γ(°)</td><td align="center">120</td><td align="center">120</td><td align="center">120</td><td align="center">120</td></tr><tr><td align="left"> V<sub>m</sub> (Å<sup>3</sup>/Da)</td><td align="center">2.57</td><td align="center">2.64</td><td align="center">2.57</td><td align="center">2.64</td></tr><tr><td align="left"> Solvent Content (%)</td><td align="center">52</td><td align="center">53</td><td align="center">52</td><td align="center">53</td></tr><tr><td align="left">X-Ray Data<xref rid="t1fn0006" ref-type="fn"><sup>2</sup></xref></td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Resolution (Å)</td><td align="center">30-2.7 (2.8-2.7)</td><td align="center">30-2.3 (2.4-2.3)</td><td align="center">30-2.2 (2.3-2.0)</td><td align="center">30-2.5 (2.6-2.5)</td></tr><tr><td align="left"> Measured Reflections</td><td align="center">297,367 (19,369)</td><td align="center">333,739 (8,008)</td><td align="center">381,125 (1,591)</td><td align="center">137,992 (9,883)</td></tr><tr><td align="left"> Unique Reflections</td><td align="center">14,402 (1,003)</td><td align="center">21,683 (1,135)</td><td align="center">24,323 (964)</td><td align="center">16,727 (1,227)</td></tr><tr><td align="left"> Completeness (%)</td><td align="center">99.6 (96.8)</td><td align="center">93.8 (68.4)</td><td align="center">95.3 (52.0)</td><td align="center">98.6 (98.1)</td></tr><tr><td align="left"> Redundancy</td><td align="center">20.6 (19.3)</td><td align="center">15.4 (7.1)</td><td align="center">15.7 (1.7)</td><td align="center">8.2 (8.1)</td></tr><tr><td align="left"> R<sub>merge</sub></td><td align="center">0.095 (0.451)</td><td align="center">0.057 (0.324)</td><td align="center">0.062 (0.406)</td><td align="center">0.047 (0.445)</td></tr><tr><td align="left"> < I/σ ></td><td align="center">38.3 (8.1)</td><td align="center">36.7 (5.5)</td><td align="center">36.2 (1.6)</td><td align="center">31.6 (5.6)</td></tr><tr><td align="left"> B-factor (Å<sup>2</sup>)</td><td align="center">33.2</td><td align="center">37.3</td><td align="center">33.7</td><td align="center">54.8</td></tr><tr><td align="left">Refinement</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Resolution (Å)</td><td align="center">15-2.7</td><td align="center">15-2.3</td><td align="center">15-2.2</td><td align="center">15-2.5</td></tr><tr><td align="left"> Number of Reflections</td><td align="center">13,583</td><td align="center">20,255</td><td align="center">24,962</td><td align="center">15,811</td></tr><tr><td align="left"> Number of All Atoms</td><td align="center">3,335</td><td align="center">3,271</td><td align="center">3,298</td><td align="center">3,239</td></tr><tr><td align="left"> Number of Waters</td><td align="center">88</td><td align="center">70</td><td align="center">71</td><td align="center">21</td></tr><tr><td align="left"> R-factor (%)</td><td align="center">19.1</td><td align="center">29.8</td><td align="center">22.8</td><td align="center">25.0</td></tr><tr><td align="left"> R-free (%)</td><td align="center">26.4</td><td align="center">38.3</td><td align="center">26.6</td><td align="center">33.7</td></tr><tr><td align="left">RMSD</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Bond Lengths (Å)</td><td align="center">0.008</td><td align="center">0.005</td><td align="center">0.005</td><td align="center">0.006</td></tr><tr><td align="left"> Bond Angles (°)</td><td align="center">1.2</td><td align="center">1.0</td><td align="center">1.0</td><td align="center">1.1</td></tr><tr><td align="left"> Mean B-factor (Å<sup>2</sup>)</td><td align="center">49.1</td><td align="center">46.3</td><td align="center">51.7</td><td align="center">88.9</td></tr><tr><td align="left">Ramachandran Plot (%)</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Outliers</td><td align="center">0.2</td><td align="center">0.2</td><td align="center">0.2</td><td align="center">1.2</td></tr><tr><td align="left"> Favored</td><td align="center">96.7</td><td align="center">97.1</td><td align="center">96.5</td><td align="center">90.9</td></tr></tbody></table>
14279Fab H3-53:L1-39 H3-53:L3-11 H3-53:L3-20 H3-53:L4-1 PDB indentifier 5I1E 5I1G 5I1H 5I1I Crystal Data Crystallization Solution Buffer, pH No buffer 0.1 M Na Acetate pH 4.5 0.1 M Na Acetate pH 4.5 0.1M MES, pH 6.5 Precipitant1 16% PEG 3350 25% PEG 3350 19% PEG 4000 17% PEG 3350 Additive1 0.2 M Amm Sulfate 5% Dioxane 0.2 M Li2SO4 0.2 M Amm Sulfate 0.2 M Na Formate, 5% MPD Space Group P6522 P6522 P6522 P31 Molecules/AU 1 1 1 1 Unit Cell a(Å) 89.4 88.1 89.4 68.1 b(Å) 89.4 88.1 89.4 68.1 c(Å) 212.4 219.6 211.7 95.6 β(°) 90 90 90 90 γ(°) 120 120 120 120 Vm (Å3/Da) 2.57 2.64 2.57 2.64 Solvent Content (%) 52 53 52 53 X-Ray Data2 Resolution (Å) 30-2.7 (2.8-2.7) 30-2.3 (2.4-2.3) 30-2.2 (2.3-2.0) 30-2.5 (2.6-2.5) Measured Reflections 297,367 (19,369) 333,739 (8,008) 381,125 (1,591) 137,992 (9,883) Unique Reflections 14,402 (1,003) 21,683 (1,135) 24,323 (964) 16,727 (1,227) Completeness (%) 99.6 (96.8) 93.8 (68.4) 95.3 (52.0) 98.6 (98.1) Redundancy 20.6 (19.3) 15.4 (7.1) 15.7 (1.7) 8.2 (8.1) Rmerge 0.095 (0.451) 0.057 (0.324) 0.062 (0.406) 0.047 (0.445) < I/σ > 38.3 (8.1) 36.7 (5.5) 36.2 (1.6) 31.6 (5.6) B-factor (Å2) 33.2 37.3 33.7 54.8 Refinement Resolution (Å) 15-2.7 15-2.3 15-2.2 15-2.5 Number of Reflections 13,583 20,255 24,962 15,811 Number of All Atoms 3,335 3,271 3,298 3,239 Number of Waters 88 70 71 21 R-factor (%) 19.1 29.8 22.8 25.0 R-free (%) 26.4 38.3 26.6 33.7 RMSD Bond Lengths (Å) 0.008 0.005 0.005 0.006 Bond Angles (°) 1.2 1.0 1.0 1.1 Mean B-factor (Å2) 49.1 46.3 51.7 88.9 Ramachandran Plot (%) Outliers 0.2 0.2 0.2 1.2 Favored 96.7 97.1 96.5 90.9 d37e1371.xmlt0001TABLEtable_footnote16142Abbreviations: Amm, ammonium; PEG, polyethylene glycol.d37e1371.xmlt0001TABLEtable_footnote16198Values for high-resolution shell are in parentheses.d37e1852.xmlt0001TABLEtable_caption16251(Continued) Crystal data, X-ray data, and refinement statistics.0.9933119evidencecleaner02023-06-28T19:16:05ZDUMMY:Crystal data0.98985547evidencecleaner02023-06-28T19:16:08ZDUMMY:X-ray dataevidenceDUMMY:cleaner02023-06-28T19:16:20Zrefinement statisticsd37e1852.xmlt0001TABLEtable<?xml version="1.0" encoding="UTF-8"?>
<table frame="hsides" rules="groups"><colgroup><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/></colgroup><thead><tr><th align="left">Fab<hr/></th><th align="center">H5-51:L1-39<hr/></th><th align="center">H5-51:L3-11<hr/></th><th align="center">H5-51:L3-20<hr/></th><th align="center">H5-51:L4-1<hr/></th></tr><tr><th align="left"><italic> PDB identifier</italic></th><th align="center">4KMT</th><th align="center">5I1J</th><th align="center">5I1K</th><th align="center">5I1L</th></tr></thead><tbody><tr><td align="left">Crystal Data</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left">Crystallization Solution</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Buffer, pH</td><td align="center">0.1 M CHES, pH 9.5</td><td align="center">0.1 M Tris, pH 8.5</td><td align="center">0.1 M CHES, pH 9.5</td><td align="center">0.1 M Tris, pH 8.5</td></tr><tr><td align="left"> Precipitant<xref rid="t1fn0007" ref-type="fn"><sup>1</sup></xref></td><td align="center">1.8 M Amm Sulfate</td><td align="center">25% PEG 3350</td><td align="center">1.0 M Amm Sulfate</td><td align="center">24% PEG 3350</td></tr><tr><td align="left"> Additive<xref rid="t1fn0007" ref-type="fn"><sup>1</sup></xref></td><td align="center">5% dioxane</td><td align="center">0.2 M MgCl<sub>2</sub></td><td align="left"> </td><td align="center">0.2 M Amm Sulfate</td></tr><tr><td align="left"> Space Group</td><td align="center">P2<sub>1</sub>2<sub>1</sub>2<sub>1</sub></td><td align="center">P2<sub>1</sub>2<sub>1</sub>2<sub>1</sub></td><td align="center">P2<sub>1</sub>2<sub>1</sub>2<sub>1</sub></td><td align="center">P2<sub>1</sub></td></tr><tr><td align="left"> Molecules/AU</td><td align="center">1</td><td align="center">1</td><td align="center">1</td><td align="center">2</td></tr><tr><td align="left">Unit Cell</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> a(Å)</td><td align="center">63.7</td><td align="center">64.1</td><td align="center">63.8</td><td align="center">106.0</td></tr><tr><td align="left"> b(Å)</td><td align="center">73.8</td><td align="center">73.8</td><td align="center">74.1</td><td align="center">38.0</td></tr><tr><td align="left"> c(Å)</td><td align="center">103.1</td><td align="center">103.0</td><td align="center">103.0</td><td align="center">112.3</td></tr><tr><td align="left"> β(°)</td><td align="center">90</td><td align="center">90</td><td align="center">90</td><td align="center">100.4</td></tr><tr><td align="left"> V<sub>m</sub> (Å<sup>3</sup>/Da)</td><td align="center">2.53</td><td align="center">2.56</td><td align="center">2.54</td><td align="center">2.28</td></tr><tr><td align="left"> Solvent Content (%)</td><td align="center">51</td><td align="center">52</td><td align="center">51</td><td align="center">46</td></tr><tr><td align="left">X-Ray Data<xref rid="t1fn0008" ref-type="fn"><sup>2</sup></xref></td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Resolution (Å)</td><td align="center">30-2.1 (2.2-2.1)</td><td align="center">30-2.5 (2.6-2.5)</td><td align="center">30-1.65 (1.7-1.65)</td><td align="center">30-1.95 (2.0-1.95)</td></tr><tr><td align="left"> Measured Reflections</td><td align="center">131,839 (6,655)</td><td align="center">120,521 (7,988)</td><td align="center">246,750 (4,142)</td><td align="center">320,324 (12,119)</td></tr><tr><td align="left"> Unique Reflections</td><td align="center">27,026 (1,885)</td><td align="center">17,286 (1,236)</td><td align="center">53,058 (2,141)</td><td align="center">61,554 (3,243)</td></tr><tr><td align="left"> Completeness (%)</td><td align="center">93.6 (89.8)</td><td align="center">99.7 (97.3)</td><td align="center">89.8 (49.8)</td><td align="center">94.4 (67.1)</td></tr><tr><td align="left"> Redundancy</td><td align="center">4.9 (3.5)</td><td align="center">7.0 (6.5)</td><td align="center">4.7 (1.9)</td><td align="center">5.2 (3.7)</td></tr><tr><td align="left"> R<sub>merge</sub></td><td align="center">0.079 (0.278)</td><td align="center">0.080 (0.281)</td><td align="center">0.034 (0.131)</td><td align="center">0.060 (0.395)</td></tr><tr><td align="left"> < I/σ ></td><td align="center">16.8 (5.7)</td><td align="center">21.1(6.9)</td><td align="center">27.5 (5.8)</td><td align="center">19.7 (3.1)</td></tr><tr><td align="left"> B-factor (Å<sup>2</sup>)</td><td align="center">26.0</td><td align="center">27.0</td><td align="center">21.6</td><td align="center">31.4</td></tr><tr><td align="left">Refinement</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Resolution (Å)</td><td align="center">15-2.1</td><td align="center">15-2.5</td><td align="center">15-1.65</td><td align="center">15-1.95</td></tr><tr><td align="left"> Number of Reflections</td><td align="center">25,857</td><td align="center">16,328</td><td align="center">51,882</td><td align="center">60,181</td></tr><tr><td align="left"> Number of All Atoms</td><td align="center">3,676</td><td align="center">3,454</td><td align="center">3,814</td><td align="center">7,175</td></tr><tr><td align="left"> Number of Waters</td><td align="center">302</td><td align="center">196</td><td align="center">527</td><td align="center">445</td></tr><tr><td align="left"> R-factor (%)</td><td align="center">17.1</td><td align="center">17.7</td><td align="center">17.2</td><td align="center">19.4</td></tr><tr><td align="left"> R-free (%)</td><td align="center">22.0</td><td align="center">25.8</td><td align="center">19.7</td><td align="center">25.8</td></tr><tr><td align="left">RMSD</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Bond Lengths (Å)</td><td align="center">0.006</td><td align="center">0.009</td><td align="center">0.005</td><td align="center">0.009</td></tr><tr><td align="left"> Bond Angles (°)</td><td align="center">1.0</td><td align="center">1.3</td><td align="center">1.3</td><td align="center">1.3</td></tr><tr><td align="left"> Mean B-factor (Å<sup>2</sup>)</td><td align="center">25.2</td><td align="center">38.2</td><td align="center">20.0</td><td align="center">19.5</td></tr><tr><td align="left">Ramachandran Plot (%)</td><td align="left"> </td><td align="left"> </td><td align="left"> </td><td align="left"> </td></tr><tr><td align="left"> Outliers</td><td align="center">0.0</td><td align="center">0.0</td><td align="center">0.0</td><td align="center">0.0</td></tr><tr><td align="left"> Favored</td><td align="center">98.4</td><td align="center">97.9</td><td align="center">98.1</td><td align="center">98.0</td></tr></tbody></table>
16316Fab H5-51:L1-39 H5-51:L3-11 H5-51:L3-20 H5-51:L4-1 PDB identifier 4KMT 5I1J 5I1K 5I1L Crystal Data Crystallization Solution Buffer, pH 0.1 M CHES, pH 9.5 0.1 M Tris, pH 8.5 0.1 M CHES, pH 9.5 0.1 M Tris, pH 8.5 Precipitant1 1.8 M Amm Sulfate 25% PEG 3350 1.0 M Amm Sulfate 24% PEG 3350 Additive1 5% dioxane 0.2 M MgCl2 0.2 M Amm Sulfate Space Group P212121 P212121 P212121 P21 Molecules/AU 1 1 1 2 Unit Cell a(Å) 63.7 64.1 63.8 106.0 b(Å) 73.8 73.8 74.1 38.0 c(Å) 103.1 103.0 103.0 112.3 β(°) 90 90 90 100.4 Vm (Å3/Da) 2.53 2.56 2.54 2.28 Solvent Content (%) 51 52 51 46 X-Ray Data2 Resolution (Å) 30-2.1 (2.2-2.1) 30-2.5 (2.6-2.5) 30-1.65 (1.7-1.65) 30-1.95 (2.0-1.95) Measured Reflections 131,839 (6,655) 120,521 (7,988) 246,750 (4,142) 320,324 (12,119) Unique Reflections 27,026 (1,885) 17,286 (1,236) 53,058 (2,141) 61,554 (3,243) Completeness (%) 93.6 (89.8) 99.7 (97.3) 89.8 (49.8) 94.4 (67.1) Redundancy 4.9 (3.5) 7.0 (6.5) 4.7 (1.9) 5.2 (3.7) Rmerge 0.079 (0.278) 0.080 (0.281) 0.034 (0.131) 0.060 (0.395) < I/σ > 16.8 (5.7) 21.1(6.9) 27.5 (5.8) 19.7 (3.1) B-factor (Å2) 26.0 27.0 21.6 31.4 Refinement Resolution (Å) 15-2.1 15-2.5 15-1.65 15-1.95 Number of Reflections 25,857 16,328 51,882 60,181 Number of All Atoms 3,676 3,454 3,814 7,175 Number of Waters 302 196 527 445 R-factor (%) 17.1 17.7 17.2 19.4 R-free (%) 22.0 25.8 19.7 25.8 RMSD Bond Lengths (Å) 0.006 0.009 0.005 0.009 Bond Angles (°) 1.0 1.3 1.3 1.3 Mean B-factor (Å2) 25.2 38.2 20.0 19.5 Ramachandran Plot (%) Outliers 0.0 0.0 0.0 0.0 Favored 98.4 97.9 98.1 98.0 d37e1852.xmlt0001TABLEtable_footnote18144Abbreviations: Amm, ammonium; PEG, polyethylene glycol.d37e1852.xmlt0001TABLEtable_footnote18200Values for high-resolution shell are in parentheses.RESULTSparagraph18253The crystal structures of a germline library composed of 16 Fabs generated by combining 4 HCs (H1-69, H3-23, H3-53 and H5-51) and 4 LCs (L1-39, L3-11, L3-20 and L4-1) have been determined. The Fab heavy and light chain sequences for the variants numbered according to Chothia are shown in Fig. S1. The four different HCs all have the same CDR H3 sequence, ARYDGIYGELDF. Crystallization of the 16 Fabs was previously reported. Three sets of the crystals were isomorphous with nearly identical unit cells (Table 1). These include (1) H3-23:L3-11 and H3-23:L4-1 in P212121, (2) H3-53:L1-39, H3-53:L3-11 and H3-53:L3-20 in P6522, and (3) H5-51:L1-39, H5-51:L3-11 and H5-51:L3-20 in P212121. Crystallization conditions for the 3 groups are also similar, but not identical (Table 1). Variations occur in the pH (buffer) and the additives, and, in group 3, PEG 3350 is the precipitant for one variants while ammonium sulfate is the precipitant for the other two. The similarity in the crystal forms is attributed in part to cross-seeding using the microseed matrix screening for groups 2 and 3.0.9973289evidencecleaner02023-06-28T19:16:52ZDUMMY:crystal structuresexperimental_methodMESH:cleaner02023-06-29T09:07:12Zgermline library0.15716633structure_elementcleaner02023-06-28T15:58:46ZSO:Fabs0.9913346structure_elementcleaner02023-06-28T16:11:32ZSO:HCsmutantMESH:cleaner02023-06-28T21:03:19ZH1-690.54475325mutantcleaner02023-06-28T21:04:17ZMESH:H3-230.56800383mutantcleaner02023-06-28T21:08:32ZMESH:H3-53mutantMESH:cleaner02023-06-28T21:05:05ZH5-510.99582016structure_elementcleaner02023-06-28T16:11:39ZSO:LCs0.78147143mutantcleaner02023-06-28T21:05:50ZMESH:L1-390.8090814mutantcleaner02023-06-28T21:06:34ZMESH:L3-110.78590775mutantcleaner02023-06-28T21:07:15ZMESH:L3-200.4724779mutantcleaner02023-06-28T21:08:00ZMESH:L4-1structure_elementSO:cleaner02023-06-28T15:59:04ZFabstructure_elementSO:cleaner02023-06-28T16:13:49Zlight chain0.97605056structure_elementcleaner02023-06-28T16:11:32ZSO:HCsstructure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T19:05:14ZH30.3840933structure_elementcleaner02023-06-29T08:56:41ZSO:ARYDGIYGELDF0.7428561experimental_methodcleaner02023-06-29T09:07:21ZMESH:Crystallization0.16106084structure_elementcleaner02023-06-28T15:58:46ZSO:Fabs0.990274evidencecleaner02023-06-28T19:17:00ZDUMMY:crystalscomplex_assemblyGO:cleaner02023-06-28T21:12:09ZH3-23:L3-11complex_assemblyGO:cleaner02023-06-28T21:12:33ZH3-23:L4-1complex_assemblyGO:cleaner02023-06-28T21:13:03ZH3-53:L1-39complex_assemblyGO:cleaner02023-06-28T21:13:26ZH3-53:L3-11complex_assemblyGO:cleaner02023-06-28T21:13:46ZH3-53:L3-20complex_assemblyGO:cleaner02023-06-28T21:14:10ZH5-51:L1-39complex_assemblyGO:cleaner02023-06-28T21:14:30ZH5-51:L3-11complex_assemblyGO:cleaner02023-06-28T21:14:52ZH5-51:L3-200.93274474chemicalcleaner02023-06-29T09:24:13ZCHEBI:PEG 33500.9821999chemicalcleaner02023-06-29T09:24:17ZCHEBI:ammonium sulfate0.94161546evidencecleaner02023-06-28T19:17:05ZDUMMY:crystal forms0.979331experimental_methodcleaner02023-06-29T09:07:27ZMESH:microseed matrix screeningRESULTSparagraph19344The crystal structures of the 16 Fabs have been determined at resolutions ranging from 3.3 Å to 1.65 Å (Table 1). The number of Fab molecules in the crystallographic asymmetric unit varies from 1 (for 12 Fabs) to 2 (for 4 Fabs). Overall the structures are fairly complete, and, as can be expected, the models for the higher resolution structures are more complete than those for the lower resolution structures (Table S1). Invariably, the HCs have more disorder than the LCs. For the LC, the disorder is observed at 2 of the C-terminal residues with few exceptions. Apart from the C-terminus, only a few surface residues in LC are disordered.0.9972463evidencecleaner02023-06-28T19:16:53ZDUMMY:crystal structures0.26109496structure_elementcleaner02023-06-28T15:58:46ZSO:Fabs0.19023296structure_elementcleaner02023-06-28T15:59:04ZSO:Fab0.30332693structure_elementcleaner02023-06-28T15:58:46ZSO:Fabs0.28805926structure_elementcleaner02023-06-28T15:58:46ZSO:Fabs0.9964563evidencecleaner02023-06-28T19:17:25ZDUMMY:structures0.9963349evidencecleaner02023-06-28T19:17:28ZDUMMY:structures0.996055evidencecleaner02023-06-28T19:17:31ZDUMMY:structures0.9984939structure_elementcleaner02023-06-28T16:11:32ZSO:HCs0.92722005protein_statecleaner02023-06-29T09:27:42ZDUMMY:disorder0.9984347structure_elementcleaner02023-06-28T16:11:39ZSO:LCs0.9984855structure_elementcleaner02023-06-28T16:24:55ZSO:LC0.8974196protein_statecleaner02023-06-29T09:27:45ZDUMMY:disorder0.9983936structure_elementcleaner02023-06-28T16:24:55ZSO:LC0.9923855protein_statecleaner02023-06-29T09:27:48ZDUMMY:disorderedRESULTSparagraph19993The HCs feature the largest number of disordered residues, with the lower resolution structures having the most. The C-terminal residues including the 6xHis tags are disordered in all 16 structures. In addition to these, 2 primary disordered stretches of residues are observed in a number of structures (Table S1). One involves the loop connecting the first 2 β-strands of the constant domain (in all Fabs except H3-23:L1-39, H3-23:L3-11 and H3-53:L1-39). The other is located in CDR H3 (in H5-51:L3-11, H5-51:L3-20 and in one of 2 copies of H3-23:L4-1). CDR H1 and CDR H2 also show some degree of disorder, but to a lesser extent.0.9985374structure_elementcleaner02023-06-28T16:11:32ZSO:HCs0.9899644protein_statecleaner02023-06-29T09:27:51ZDUMMY:disordered0.99663144evidencecleaner02023-06-28T19:17:33ZDUMMY:structures0.99440306protein_statecleaner02023-06-29T09:27:55ZDUMMY:disordered0.99707234evidencecleaner02023-06-28T19:17:36ZDUMMY:structures0.9967458evidencecleaner02023-06-28T19:17:39ZDUMMY:structures0.97793114structure_elementcleaner02023-06-29T08:56:00ZSO:loop0.9568026structure_elementcleaner02023-06-29T08:56:03ZSO:β-strands0.9960027structure_elementcleaner02023-06-29T08:56:05ZSO:constant domain0.46980876structure_elementcleaner02023-06-28T15:58:46ZSO:Fabscomplex_assemblyGO:cleaner02023-06-28T21:15:27ZH3-23:L1-39complex_assemblyGO:cleaner02023-06-28T21:12:10ZH3-23:L3-11complex_assemblyGO:cleaner02023-06-28T21:13:04ZH3-53:L1-390.9980945structure_elementcleaner02023-06-28T16:25:21ZSO:CDR0.8540226structure_elementcleaner02023-06-28T19:05:15ZSO:H3complex_assemblyGO:cleaner02023-06-28T21:14:32ZH5-51:L3-11complex_assemblyGO:cleaner02023-06-28T21:14:53ZH5-51:L3-20complex_assemblyGO:cleaner02023-06-28T21:12:34ZH3-23:L4-10.99790764structure_elementcleaner02023-06-28T16:25:21ZSO:CDR0.97477245structure_elementcleaner02023-06-28T19:04:10ZSO:H10.99792707structure_elementcleaner02023-06-28T16:25:21ZSO:CDR0.9856117structure_elementcleaner02023-06-28T16:26:21ZSO:H20.9067931protein_statecleaner02023-06-29T09:27:58ZDUMMY:disorderRESULTStitle_220628CDR canonical structures0.9833983structure_elementcleaner02023-06-28T16:25:21ZSO:CDRevidenceDUMMY:cleaner02023-06-28T20:50:47ZstructuresRESULTSparagraph20653Several CDR definitions have evolved over decades of antibody research. Depending on the focus of the study, the CDR boundaries differ slightly between various definitions. In this work, we use the CDR definition of North et al., which is similar to that of Martin with the following exceptions: 1) CDRs H1 and H3 begin immediately after the Cys; and 2) CDR L2 includes an additional residue at the N-terminal side, typically Tyr.structure_elementSO:cleaner02023-06-28T16:25:21ZCDRprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibodystructure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T16:25:21ZCDR0.9945135structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.9963715structure_elementcleaner02023-06-28T19:04:10ZSO:H10.9967776structure_elementcleaner02023-06-28T19:05:15ZSO:H30.9913113residue_namecleaner02023-06-29T09:11:56ZSO:Cys0.996126structure_elementcleaner02023-06-28T16:25:21ZSO:CDR0.99719477structure_elementcleaner02023-06-28T20:09:53ZSO:L20.9898846residue_namecleaner02023-06-29T09:11:59ZSO:TyrRESULTStitle_221085CDR H1structure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T19:04:11ZH1kmab-08-06-1190060-g001.jpgf0001FIGfig_caption21092The superposition of CDR H1 backbones for all HC:LC pairs with heavy chains: (A) H1-69, (B) H3-23, (C) H3-53 and (D) H5-51.0.99592435experimental_methodcleaner02023-06-29T09:07:33ZMESH:superpositionstructure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T19:04:11ZH10.9562733complex_assemblycleaner02023-06-29T09:23:30ZGO:HC:LCstructure_elementSO:cleaner02023-06-28T15:56:24Zheavy chains0.89844674mutantcleaner02023-06-28T21:03:19ZMESH:H1-690.9519102mutantcleaner02023-06-28T21:04:17ZMESH:H3-230.91820097mutantcleaner02023-06-28T21:08:32ZMESH:H3-530.93086475mutantcleaner02023-06-28T21:05:05ZMESH:H5-51t0002.xmlt0002TABLEtable_caption21216Canonical structures.t0002.xmlt0002TABLEtable<?xml version="1.0" encoding="UTF-8"?>
<table frame="hsides" rules="groups"><colgroup><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/></colgroup><thead><tr><th align="left">Pairs</th><th align="center">PDB</th><th align="center">CDR H1</th><th align="center">CDR H2</th><th align="center">CDR H3</th></tr></thead><tbody><tr><td align="left"><bold>H1-69</bold></td><td align="left"> </td><td align="left">KASGGTFSSYAIS</td><td align="left">GIIPIFGTAN</td><td align="left">ARYDGIYGELDF</td></tr><tr><td align="left"><bold>H1-69</bold>:L1-39</td><td align="center">5I15</td><td align="left">H1-13-4</td><td align="left">H2-10-1</td><td align="left">H3-12-1</td></tr><tr><td align="left"><bold>H1-69</bold>:L3-11</td><td align="center">5I16</td><td align="left">H1-13-1/H1-13-1</td><td align="left">H2-10-1/H2-10-1</td><td align="left">H3-12-1/H3-12-1</td></tr><tr><td align="left"><bold>H1-69</bold>:L3-20</td><td align="center">5I17</td><td align="left">H1-13-3/H1-13-6</td><td align="left">H2-10-1/NA</td><td align="left">H3-12-1/H3-12-1</td></tr><tr><td align="left"><bold>H1-69</bold>:L4-1</td><td align="center">5I18</td><td align="left">H1-13-10</td><td align="left">H2-10-1</td><td align="left">H3-12-1</td></tr><tr><td align="left"><bold>H3-23</bold></td><td align="left"> </td><td align="left">AASGFTFSSYAMS</td><td align="left">AISGSGGSTY</td><td align="left">AKYDGIYDGIYGELDF</td></tr><tr><td align="left"><bold>H3-23</bold>:L1-39</td><td align="center">5I19</td><td align="left">H1-13-1</td><td align="left">H2-10-2</td><td align="left">H3-12-1</td></tr><tr><td align="left"><bold>H3-23</bold>:L3-11</td><td align="center">5I1A</td><td align="left">H1-13-1/H1-13-1</td><td align="left">H2-10-2/H2-10-2</td><td align="left">H3-12-1/H3-12-1</td></tr><tr><td align="left"><bold>H3-23</bold>:L3-20</td><td align="center">5I1C</td><td align="left">H1-13-1</td><td align="left">H2-10-2</td><td align="left">H3-12-1</td></tr><tr><td align="left"><bold>H3-23</bold>:L4-1</td><td align="center">5I1D</td><td align="left">H1-13-1/H1-13-1</td><td align="left">H2-10-2/H2-10-2</td><td align="left">H3-12-1/NA</td></tr><tr><td align="left"><bold>H3-53</bold></td><td align="left"> </td><td align="left">AASGFTVSSNYMS</td><td align="left">VIYSGGSTY</td><td align="left">ARYDGIYGELDF</td></tr><tr><td align="left"><bold>H3-53</bold>:L1-39</td><td align="center">5I1E</td><td align="left">H1-13-1</td><td align="left">H2-9-3</td><td align="left">H3-12-1</td></tr><tr><td align="left"><bold>H3-53</bold>:L3-11</td><td align="center">5I1G</td><td align="left">H1-13-1</td><td align="left">H2-9-3</td><td align="left">H3-12-1</td></tr><tr><td align="left"><bold>H3-53</bold>:L3-20</td><td align="center">5I1H</td><td align="left">H1-13-1</td><td align="left">H2-9-3</td><td align="left">H3-12-1</td></tr><tr><td align="left"><bold>H3-53</bold>:L4-1</td><td align="center">5I1I</td><td align="left">H1-13-1</td><td align="left">H2-9-3</td><td align="left">NA</td></tr><tr><td align="left"><bold>H5-51</bold></td><td align="left"> </td><td align="left">KGSGYSFTSYWIG</td><td align="left">IIYPGDSDTR</td><td align="left">ARYDGIYGELDF</td></tr><tr><td align="left"><bold>H5-51</bold>:L1-39</td><td align="center">4KMT</td><td align="left">H1-13-1</td><td align="left">H2-10-1</td><td align="left">H3-12-1</td></tr><tr><td align="left"><bold>H5-51</bold>:L3-11</td><td align="center">5I1J</td><td align="left">H1-13-1</td><td align="left">H2-10-1</td><td align="left">NA</td></tr><tr><td align="left"><bold>H5-51</bold>:L3-20</td><td align="center">5I1K</td><td align="left">H1-13-1</td><td align="left">H2-10-1</td><td align="left">NA</td></tr><tr><td align="left"><bold>H5-51</bold>:L4-1</td><td align="center">5I1L</td><td align="left">H1-13-1/H1-13-1</td><td align="left">H2-10-1/H2-10-1</td><td align="left">H3-12-1/H3-12-1</td></tr><tr><td align="left"> </td><td align="left"> </td><td align="left"><bold>CDR L1</bold></td><td align="left"><bold>CDR L2</bold></td><td align="left"><bold>CDR L3</bold></td></tr><tr><td align="left"><bold>L1-39</bold></td><td align="left"> </td><td align="left">RASQSISSYLN</td><td align="left">YAASSLQS</td><td align="left">QQSYSTPLT</td></tr><tr><td align="left">H1-69:<bold>L1-39</bold></td><td align="center">5I15</td><td align="left">L1-11-1</td><td align="left">L2-8-1</td><td align="left">L3-9-cis7-1</td></tr><tr><td align="left">H3-23:<bold>L1-39</bold></td><td align="center">5I19</td><td align="left">L1-11-1</td><td align="left">L2-8-1</td><td align="left">L3-9-cis7-1</td></tr><tr><td align="left">H3-53:<bold>L1-39</bold></td><td align="center">5I1E</td><td align="left">L1-11-1</td><td align="left">L2-8-1</td><td align="left">L3-9-cis7-1</td></tr><tr><td align="left">H5-51:<bold>L1-39</bold></td><td align="center">4KMT</td><td align="left">L1-11-1</td><td align="left">L2-8-1</td><td align="left">L3-9-cis7-1</td></tr><tr><td align="left"><bold>L3-11</bold></td><td align="left"> </td><td align="left">RASQSVSSYLA</td><td align="left">YDASNRAT</td><td align="left">QQRSNWPLT</td></tr><tr><td align="left">H1-69:<bold>L3-11</bold></td><td align="center">5I16</td><td align="left">L1-11-1/L1-11-1</td><td align="left">L2-8-1/L2-8-1</td><td align="left">L3-9-cis7-1/L3-9-cis7-1</td></tr><tr><td align="left">H3-23:<bold>L3-11</bold></td><td align="center">5I1A</td><td align="left">L1-11-1/L1-11-1</td><td align="left">L2-8-1/L2-8-1</td><td align="left">L3-9-cis7-1/L3-9-cis7-1</td></tr><tr><td align="left">H3-53:<bold>L3-11</bold></td><td align="center">5I1G</td><td align="left">L1-11-1</td><td align="left">L2-8-1</td><td align="left">L3-9-cis7-1</td></tr><tr><td align="left">H5-51:<bold>L3-11</bold></td><td align="center">5I1J</td><td align="left">L1-11-1</td><td align="left">L2-8-1</td><td align="left">L3-9-cis7-1</td></tr><tr><td align="left"><bold>L3-20</bold></td><td align="left"> </td><td align="left">RASQSVSSSYLA</td><td align="left">YGASSRAT</td><td align="left">QQYGSSPLT</td></tr><tr><td align="left">H1-69:<bold>L3-20</bold></td><td align="center">5I17</td><td align="left">L1-12-2/L1-12-1</td><td align="left">L2-8-1/L2-8-1</td><td align="left">L3-9-cis7-1/L3-9-cis7-1</td></tr><tr><td align="left">H3-23:<bold>L3-20</bold></td><td align="center">5I1C</td><td align="left">L1-12-2</td><td align="left">L2-8-1</td><td align="left">L3-9-cis7-1</td></tr><tr><td align="left">H3-53:<bold>L3-20</bold></td><td align="center">5I1H</td><td align="left">L1-12-1</td><td align="left">L2-8-1</td><td align="left">L3-9-cis7-1</td></tr><tr><td align="left">H5-51:<bold>L3-20</bold></td><td align="center">5I1K</td><td align="left">L1-12-1</td><td align="left">L2-8-1</td><td align="left">L3-9-cis7-1</td></tr><tr><td align="left"><bold>L4-1</bold></td><td align="left"> </td><td align="left">KSSQSVLYSSNNKNYLA</td><td align="left">YWASTRES</td><td align="left">QQYYSTPLT</td></tr><tr><td align="left">H1-69:<bold>L4-1</bold></td><td align="center">5I18</td><td align="left">L1-17-1</td><td align="left">L2-8-1</td><td align="left">L3-9-cis7-1</td></tr><tr><td align="left">H3-23:<bold>L4-1</bold></td><td align="center">5I1D</td><td align="left">L1-17-1/L1-17-1</td><td align="left">L2-8-1/L2-8-1</td><td align="left">L3-9-cis7-1/L3-9-cis7-1</td></tr><tr><td align="left">H3-53:<bold>L4-1</bold></td><td align="center">5I1I</td><td align="left">L1-17-1</td><td align="left">L2-8-1</td><td align="left">L3-9-cis7-1</td></tr><tr><td align="left">H5-51:<bold>L4-1</bold></td><td align="center">5I1L</td><td align="left">L1-17-1/L1-17-1</td><td align="left">L2-8-1/L2-8-1</td><td align="left">L3-9-cis7-1/L3-9-cis7-1</td></tr></tbody></table>
21238Pairs PDB CDR H1 CDR H2 CDR H3 H1-69 KASGGTFSSYAIS GIIPIFGTAN ARYDGIYGELDF H1-69:L1-39 5I15 H1-13-4 H2-10-1 H3-12-1 H1-69:L3-11 5I16 H1-13-1/H1-13-1 H2-10-1/H2-10-1 H3-12-1/H3-12-1 H1-69:L3-20 5I17 H1-13-3/H1-13-6 H2-10-1/NA H3-12-1/H3-12-1 H1-69:L4-1 5I18 H1-13-10 H2-10-1 H3-12-1 H3-23 AASGFTFSSYAMS AISGSGGSTY AKYDGIYDGIYGELDF H3-23:L1-39 5I19 H1-13-1 H2-10-2 H3-12-1 H3-23:L3-11 5I1A H1-13-1/H1-13-1 H2-10-2/H2-10-2 H3-12-1/H3-12-1 H3-23:L3-20 5I1C H1-13-1 H2-10-2 H3-12-1 H3-23:L4-1 5I1D H1-13-1/H1-13-1 H2-10-2/H2-10-2 H3-12-1/NA H3-53 AASGFTVSSNYMS VIYSGGSTY ARYDGIYGELDF H3-53:L1-39 5I1E H1-13-1 H2-9-3 H3-12-1 H3-53:L3-11 5I1G H1-13-1 H2-9-3 H3-12-1 H3-53:L3-20 5I1H H1-13-1 H2-9-3 H3-12-1 H3-53:L4-1 5I1I H1-13-1 H2-9-3 NA H5-51 KGSGYSFTSYWIG IIYPGDSDTR ARYDGIYGELDF H5-51:L1-39 4KMT H1-13-1 H2-10-1 H3-12-1 H5-51:L3-11 5I1J H1-13-1 H2-10-1 NA H5-51:L3-20 5I1K H1-13-1 H2-10-1 NA H5-51:L4-1 5I1L H1-13-1/H1-13-1 H2-10-1/H2-10-1 H3-12-1/H3-12-1 CDR L1 CDR L2 CDR L3 L1-39 RASQSISSYLN YAASSLQS QQSYSTPLT H1-69:L1-39 5I15 L1-11-1 L2-8-1 L3-9-cis7-1 H3-23:L1-39 5I19 L1-11-1 L2-8-1 L3-9-cis7-1 H3-53:L1-39 5I1E L1-11-1 L2-8-1 L3-9-cis7-1 H5-51:L1-39 4KMT L1-11-1 L2-8-1 L3-9-cis7-1 L3-11 RASQSVSSYLA YDASNRAT QQRSNWPLT H1-69:L3-11 5I16 L1-11-1/L1-11-1 L2-8-1/L2-8-1 L3-9-cis7-1/L3-9-cis7-1 H3-23:L3-11 5I1A L1-11-1/L1-11-1 L2-8-1/L2-8-1 L3-9-cis7-1/L3-9-cis7-1 H3-53:L3-11 5I1G L1-11-1 L2-8-1 L3-9-cis7-1 H5-51:L3-11 5I1J L1-11-1 L2-8-1 L3-9-cis7-1 L3-20 RASQSVSSSYLA YGASSRAT QQYGSSPLT H1-69:L3-20 5I17 L1-12-2/L1-12-1 L2-8-1/L2-8-1 L3-9-cis7-1/L3-9-cis7-1 H3-23:L3-20 5I1C L1-12-2 L2-8-1 L3-9-cis7-1 H3-53:L3-20 5I1H L1-12-1 L2-8-1 L3-9-cis7-1 H5-51:L3-20 5I1K L1-12-1 L2-8-1 L3-9-cis7-1 L4-1 KSSQSVLYSSNNKNYLA YWASTRES QQYYSTPLT H1-69:L4-1 5I18 L1-17-1 L2-8-1 L3-9-cis7-1 H3-23:L4-1 5I1D L1-17-1/L1-17-1 L2-8-1/L2-8-1 L3-9-cis7-1/L3-9-cis7-1 H3-53:L4-1 5I1I L1-17-1 L2-8-1 L3-9-cis7-1 H5-51:L4-1 5I1L L1-17-1/L1-17-1 L2-8-1/L2-8-1 L3-9-cis7-1/L3-9-cis7-1 t0002.xmlt0002TABLEtable_footnote23317CDRs are defined using the Dunbrack convention [12]. Assignments for 2 copies of the Fab in the asymmetric unit are given for 5 structures. No assignment (NA) for CDRs with missing residues.0.9826041structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.34516945structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.9959evidencecleaner02023-06-28T20:07:21ZDUMMY:structures0.97968894structure_elementcleaner02023-06-28T19:04:38ZSO:CDRsRESULTSparagraph23508The four HCs feature CDR H1 of the same length, and their sequences are highly similar (Table 2). The CDR H1 backbone conformations for all variants for each of the HCs are shown in Fig. 1. Three of the HCs, H3-23, H3-53 and H5-51, have the same canonical structure, H1-13-1, and the backbone conformations are tightly clustered for each set of Fab structures as reflected in the rmsd values (Fig. 1B-D). Some deviation is observed for H3-53, mostly due to H3-53:L4-1, which exhibits a significant degree of disorder in CDR H1. The electron density for the backbone is weak and discontinuous, and completely missing for several side chains. 0.99611866structure_elementcleaner02023-06-28T16:11:33ZSO:HCsstructure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T19:04:11ZH1structure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T19:04:11ZH10.9957355structure_elementcleaner02023-06-28T16:11:33ZSO:HCs0.9940059structure_elementcleaner02023-06-28T16:11:33ZSO:HCs0.83604187mutantcleaner02023-06-28T21:04:17ZMESH:H3-230.8691897mutantcleaner02023-06-28T21:08:32ZMESH:H3-530.8163323mutantcleaner02023-06-28T21:05:05ZMESH:H5-510.6929795mutantcleaner02023-06-28T21:17:48ZMESH:H1-13-10.22198454structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.9957462evidencecleaner02023-06-28T20:07:16ZDUMMY:structures0.9691633evidencecleaner02023-06-28T20:07:24ZDUMMY:rmsd values0.8695476mutantcleaner02023-06-28T21:08:32ZMESH:H3-53complex_assemblyGO:cleaner02023-06-28T21:18:19ZH3-53:L4-1structure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T19:04:11ZH10.9966054evidencecleaner02023-06-28T20:07:28ZDUMMY:electron densityRESULTSparagraph24155The CDR H1 structures with H1-69 shown in Fig. 1A are quite variable, both for the structures with different LCs and for the copies of the same Fab in the asymmetric unit, H1-69:L3-11 and H1-69:L3-20. In total, 6 independent Fab structures produce 5 different canonical structures, namely H1-13-1, H1-13-3, H1-13-4, H1-13-6 and H1-13-10. A major difference of H1-69 from the other germlines in the experimental data set is the presence of Gly instead of Phe or Tyr at position 27 (residue 5 of 13 in CDR H1). Glycine introduces the possibility of a higher degree of conformational flexibility that undoubtedly translates to the differences observed, and contributes to the elevated thermal parameters for the atoms in the amino acid residues in this region.structure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T19:04:11ZH10.99516606evidencecleaner02023-06-28T20:07:31ZDUMMY:structuresmutantMESH:cleaner02023-06-28T21:03:19ZH1-690.99432063evidencecleaner02023-06-28T20:07:34ZDUMMY:structures0.99687505structure_elementcleaner02023-06-28T16:11:39ZSO:LCs0.21805374structure_elementcleaner02023-06-28T15:59:05ZSO:Fabcomplex_assemblyGO:cleaner02023-06-28T21:18:58ZH1-69:L3-11complex_assemblyGO:cleaner02023-06-28T21:20:20ZH1-69:L3-200.36521375structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.99359375evidencecleaner02023-06-28T20:07:37ZDUMMY:structures0.982238evidencecleaner02023-06-28T20:07:40ZDUMMY:structures0.97110045mutantcleaner02023-06-29T09:25:57ZMESH:H1-13-10.97449195mutantcleaner02023-06-29T09:26:01ZMESH:H1-13-30.9718064mutantcleaner02023-06-29T09:26:04ZMESH:H1-13-40.96956253mutantcleaner02023-06-29T09:26:07ZMESH:H1-13-60.9710226mutantcleaner02023-06-29T09:26:10ZMESH:H1-13-100.78516006mutantcleaner02023-06-28T21:03:20ZMESH:H1-690.9891431residue_namecleaner02023-06-29T09:12:05ZSO:Gly0.9896833residue_namecleaner02023-06-29T09:12:08ZSO:Phe0.9894337residue_namecleaner02023-06-29T09:12:10ZSO:Tyr0.956861residue_numbercleaner02023-06-29T09:19:14ZDUMMY:27structure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T19:04:11ZH10.9914665residue_namecleaner02023-06-29T09:12:14ZSO:GlycineRESULTStitle_224914CDR H2structure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T16:26:21ZH2kmab-08-06-1190060-g002.jpgf0002FIGfig_caption24921The superposition of CDR H2 backbones for all HC:LC pairs with heavy chains: (A) H1-69, (B) H3-23, (C) H3-53 and (D) H5-51.0.99599445experimental_methodcleaner02023-06-29T09:07:39ZMESH:superpositionstructure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T16:26:21ZH20.9441673complex_assemblycleaner02023-06-29T09:23:36ZGO:HC:LCstructure_elementSO:cleaner02023-06-28T15:56:24Zheavy chains0.91638017mutantcleaner02023-06-28T21:03:20ZMESH:H1-690.94655305mutantcleaner02023-06-28T21:04:17ZMESH:H3-230.9128931mutantcleaner02023-06-28T21:08:32ZMESH:H3-530.91781205mutantcleaner02023-06-28T21:05:05ZMESH:H5-51RESULTSparagraph25045The canonical structures of CDR H2 have fairly consistent conformations (Table 2, Fig. 2). Each of the 4 HCs adopts only one canonical structure regardless of the pairing LC. Germlines H1-69 and H5-51 have the same canonical structure assignment H2-10-1, H3-23 has H2-10-2, and H3-53 has H2-9-3. The conformations for all of these CDR H2s are tightly clustered (Fig. 2). In one case, in the second Fab of H1-69:L3-20, CDR H2 is partially disordered (Δ55-60). 0.9974942structure_elementcleaner02023-06-28T16:25:21ZSO:CDR0.92632896structure_elementcleaner02023-06-28T16:26:21ZSO:H20.9952141structure_elementcleaner02023-06-28T16:11:33ZSO:HCs0.8810361structure_elementcleaner02023-06-28T16:24:55ZSO:LC0.69402623mutantcleaner02023-06-28T21:03:20ZMESH:H1-69mutantMESH:cleaner02023-06-28T21:05:05ZH5-51mutantMESH:cleaner02023-06-28T21:22:12ZH2-10-10.70856994mutantcleaner02023-06-28T21:04:17ZMESH:H3-230.70212114mutantcleaner02023-06-28T21:22:37ZMESH:H2-10-20.7003109mutantcleaner02023-06-28T21:08:32ZMESH:H3-530.79651135mutantcleaner02023-06-28T21:22:52ZMESH:H2-9-3structure_elementSO:cleaner02023-06-28T16:25:21ZCDRstructure_elementSO:cleaner02023-06-28T21:23:32ZH2s0.27054203structure_elementcleaner02023-06-28T15:59:05ZSO:Fabcomplex_assemblyGO:cleaner02023-06-28T21:20:21ZH1-69:L3-200.9976173structure_elementcleaner02023-06-28T16:25:21ZSO:CDR0.96096814structure_elementcleaner02023-06-28T16:26:21ZSO:H20.70799065protein_statecleaner02023-06-29T09:29:04ZDUMMY:partially disorderedmutantMESH:cleaner02023-06-28T21:24:07ZΔ55-60RESULTSparagraph25510Although three of the germlines have CDR H2 of the same length, 10 residues, they adopt 2 distinctively different conformations depending mostly on the residue at position 71 from the so-called CDR H4. Arg71 in H3-23 fills the space between CDRs H2 and H4, and defines the conformation of the tip of CDR H2 so that residue 54 points away from the antigen binding site. Germlines H1-69 and H5-51 are unique in the human repertoire in having an Ala at position 71 that leaves enough space for H-Pro52a to pack deeper against CDR H4 so that the following residues 53 and 54 point toward the putative antigen.0.99742055structure_elementcleaner02023-06-28T16:25:21ZSO:CDR0.81681776structure_elementcleaner02023-06-28T16:26:21ZSO:H20.62872905residue_rangecleaner02023-06-29T09:14:33ZDUMMY:10 residues0.9469733residue_numbercleaner02023-06-29T09:19:32ZDUMMY:710.9968274structure_elementcleaner02023-06-28T16:25:21ZSO:CDR0.9557131structure_elementcleaner02023-06-29T08:58:17ZSO:H40.99837995residue_name_numbercleaner02023-06-29T09:21:11ZDUMMY:Arg710.85665005mutantcleaner02023-06-28T21:04:17ZMESH:H3-230.9966505structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.99316376structure_elementcleaner02023-06-28T16:26:21ZSO:H20.9950023structure_elementcleaner02023-06-29T08:58:20ZSO:H40.9970299structure_elementcleaner02023-06-28T16:25:21ZSO:CDR0.92691565structure_elementcleaner02023-06-28T16:26:21ZSO:H20.94479257residue_numbercleaner02023-06-29T09:19:37ZDUMMY:540.9975385sitecleaner02023-06-29T09:10:35ZSO:antigen binding sitemutantMESH:cleaner02023-06-28T21:03:20ZH1-69mutantMESH:cleaner02023-06-28T21:05:05ZH5-510.9952029speciescleaner02023-06-28T16:03:59ZMESH:human0.98539925residue_namecleaner02023-06-29T09:12:19ZSO:Ala0.9392848residue_numbercleaner02023-06-29T09:19:40ZDUMMY:71structure_elementSO:cleaner02023-06-28T21:25:30ZHresidue_name_numberDUMMY:cleaner02023-06-28T21:25:20ZPro52a0.9970763structure_elementcleaner02023-06-28T16:25:21ZSO:CDR0.9823167structure_elementcleaner02023-06-29T08:58:24ZSO:H40.91533685residue_numbercleaner02023-06-29T09:19:43ZDUMMY:530.9325063residue_numbercleaner02023-06-29T09:19:46ZDUMMY:54RESULTSparagraph26116Conformations of CDR H2 in H1-69 and H5-51, both of which have canonical structure H2-10-1, show little deviation within each set of 4 structures. However, there is a significant shift of the CDR as a rigid body when the 2 sets are superimposed. Most likely this is the result of interaction of CDR H2 with CDR H1, namely with the residue at position 33 (residue 11 of 13 in CDR H1). Germline H1-69 has Ala at position 33 whereas in H5-51 position 33 is occupied by a bulky Trp, which stacks against H-Tyr52 and drives CDR H2 away from the center.0.995761structure_elementcleaner02023-06-28T16:25:21ZSO:CDR0.66203463structure_elementcleaner02023-06-28T16:26:21ZSO:H20.6642932mutantcleaner02023-06-28T21:03:20ZMESH:H1-69mutantMESH:cleaner02023-06-28T21:05:05ZH5-51mutantMESH:cleaner02023-06-28T21:26:12ZH2-10-10.99680835evidencecleaner02023-06-28T20:08:11ZDUMMY:structures0.9964696structure_elementcleaner02023-06-28T16:25:22ZSO:CDR0.9532751experimental_methodcleaner02023-06-28T20:08:15ZMESH:superimposed0.9950335structure_elementcleaner02023-06-28T16:25:22ZSO:CDR0.615978structure_elementcleaner02023-06-28T16:26:21ZSO:H20.99527454structure_elementcleaner02023-06-28T16:25:22ZSO:CDR0.874357structure_elementcleaner02023-06-28T19:04:11ZSO:H10.9602763residue_numbercleaner02023-06-29T09:19:49ZDUMMY:33structure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T19:04:11ZH1mutantMESH:cleaner02023-06-28T21:03:20ZH1-690.9874812residue_namecleaner02023-06-29T09:12:25ZSO:Ala0.9624215residue_numbercleaner02023-06-29T09:19:54ZDUMMY:33mutantMESH:cleaner02023-06-28T21:05:05ZH5-510.9566874residue_numbercleaner02023-06-29T09:19:56ZDUMMY:330.98665047residue_namecleaner02023-06-29T09:12:28ZSO:Trpstructure_elementSO:cleaner02023-06-28T21:26:43ZH0.98908526residue_name_numbercleaner02023-06-29T09:21:15ZDUMMY:Tyr520.99542576structure_elementcleaner02023-06-28T16:25:22ZSO:CDR0.57297033structure_elementcleaner02023-06-28T16:26:21ZSO:H2RESULTStitle_226664CDR L1structure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T16:26:30ZL1kmab-08-06-1190060-g003.jpgf0003FIGfig_caption26671The superposition of CDR L1 backbones for all HC:LC pairs with light chains: (A) L1-39, (B) L3-11, (C) L3-20 and (D) L4-1.0.99590635experimental_methodcleaner02023-06-29T09:07:44ZMESH:superpositionstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T16:26:30ZL10.951538complex_assemblycleaner02023-06-29T09:23:41ZGO:HC:LCstructure_elementSO:cleaner02023-06-28T16:14:08Zlight chains0.7258582mutantcleaner02023-06-28T21:05:50ZMESH:L1-390.78686476mutantcleaner02023-06-28T21:06:35ZMESH:L3-110.8088053mutantcleaner02023-06-28T21:07:16ZMESH:L3-200.78025514mutantcleaner02023-06-28T21:08:00ZMESH:L4-1RESULTSparagraph26794The four LC CDRs L1 feature 3 different lengths (11, 12 and 17 residues) having a total of 4 different canonical structure assignments. Of these LCs, L1-39 and L3-11 have the same canonical structure, L1-11-1, and superimpose very well (Fig. 3A, B). For the remaining 2, L3-20 has 2 different assignments, L1-12-1 and L1-12-2, while L4-1 has a single assignment, L1-17-1. 0.8353525structure_elementcleaner02023-06-28T16:24:55ZSO:LC0.99589455structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.9921481structure_elementcleaner02023-06-28T16:26:30ZSO:L10.7752541residue_rangecleaner02023-06-29T09:14:46ZDUMMY:110.60394007residue_rangecleaner02023-06-29T09:14:49ZDUMMY:120.53114396residue_rangecleaner02023-06-29T09:14:52ZDUMMY:170.84663665structure_elementcleaner02023-06-28T16:11:39ZSO:LCs0.86617535mutantcleaner02023-06-28T21:05:50ZMESH:L1-39mutantMESH:cleaner02023-06-28T21:06:35ZL3-110.73787403mutantcleaner02023-06-28T21:28:31ZMESH:L1-11-10.992374experimental_methodcleaner02023-06-29T09:07:49ZMESH:superimposemutantMESH:cleaner02023-06-28T21:07:16ZL3-200.91584176mutantcleaner02023-06-28T21:28:41ZMESH:L1-12-10.8342571mutantcleaner02023-06-28T21:28:51ZMESH:L1-12-2mutantMESH:cleaner02023-06-28T21:08:00ZL4-10.9382731mutantcleaner02023-06-28T21:29:01ZMESH:L1-17-1RESULTSparagraph27169L4-1 has the longest CDR L1, composed of 17 amino acid residues (Fig. 3D). Despite this, the conformations are tightly clustered (rmsd is 0.20 Å). The backbone conformations of the stem regions superimpose well. Some changes in conformation occur between residues 30a and 30f (residues 8 and 13 of 17 in CDR L1). This is the tip of the loop region, which appears to have similar conformations that fan out the structures because of the slight differences in torsion angles in the backbone near Tyr30a and Lys30f.0.97578mutantcleaner02023-06-28T21:08:00ZMESH:L4-1structure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T16:26:31ZL1residue_rangeDUMMY:cleaner02023-06-29T09:15:15Z17 amino acid residues0.99481165evidencecleaner02023-06-28T20:08:29ZDUMMY:rmsd0.9976742structure_elementcleaner02023-06-28T20:08:32ZSO:stem regionsresidue_numberDUMMY:cleaner02023-06-29T09:17:33Z30aresidue_numberDUMMY:cleaner02023-06-29T09:17:47Z30fresidue_numberDUMMY:cleaner02023-06-29T09:17:59Z8residue_numberDUMMY:cleaner02023-06-29T09:18:10Z13residue_numberDUMMY:cleaner02023-06-29T09:18:21Z17structure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T16:26:31ZL10.9900733structure_elementcleaner02023-06-28T20:08:36ZSO:loop region0.99666256evidencecleaner02023-06-28T20:08:39ZDUMMY:structures0.99906737residue_name_numbercleaner02023-06-29T09:21:20ZDUMMY:Tyr30a0.9990594residue_name_numbercleaner02023-06-29T09:21:23ZDUMMY:Lys30fRESULTSparagraph27685L3-20 is the most variable in CDR L1 among the 4 germlines as indicated by an rmsd of 0.54 Å (Fig. 3C). Two structures, H3-53:L3-20 and H5-51:L3-20 are assigned to canonical structure L1-12-1 with virtually identical backbone conformations. The third structure, H3-23:L3-20, has CDR L1 as L1-12-2, which deviates from L1-12-1 at residues 29-32, i.e., at the site of insertion with respect to the 11-residue CDR. The fourth member of the set, H1-69:L3-20, was crystallized with 2 Fabs in the asymmetric unit. The conformation of CDR L1 in these 2 Fabs is slightly different, and both conformations fall somewhere between L1-12-1 and L1-12-2. This reflects the lack of accuracy in the structure due to low resolution of the X-ray data (3.3 Å).0.44628587mutantcleaner02023-06-28T21:07:16ZMESH:L3-20structure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T16:26:31ZL10.99455017evidencecleaner02023-06-28T20:09:20ZDUMMY:rmsd0.99346644evidencecleaner02023-06-28T20:09:24ZDUMMY:structurescomplex_assemblyGO:cleaner02023-06-28T21:13:47ZH3-53:L3-20complex_assemblyGO:cleaner02023-06-28T21:14:53ZH5-51:L3-20mutantMESH:cleaner02023-06-28T21:30:26ZL1-12-10.7816724complex_assemblycleaner02023-06-28T21:30:52ZGO:H3-23:L3-20structure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T16:26:31ZL10.76978254mutantcleaner02023-06-28T21:30:02ZMESH:L1-12-20.73867476mutantcleaner02023-06-28T21:31:11ZMESH:L1-12-10.9205847residue_rangecleaner02023-06-29T09:18:29ZDUMMY:29-32residue_rangeDUMMY:cleaner02023-06-29T09:20:29Z11-residue0.997166structure_elementcleaner02023-06-28T16:25:22ZSO:CDR0.8665576complex_assemblycleaner02023-06-28T21:20:21ZGO:H1-69:L3-200.98529875experimental_methodcleaner02023-06-28T20:09:16ZMESH:crystallized0.48778555structure_elementcleaner02023-06-28T15:58:47ZSO:Fabsstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T16:26:31ZL10.28599226structure_elementcleaner02023-06-28T15:58:47ZSO:FabsmutantMESH:cleaner02023-06-28T21:31:55ZL1-12-10.7995807mutantcleaner02023-06-28T21:32:11ZMESH:L1-12-20.9954146evidencecleaner02023-06-28T20:09:28ZDUMMY:structure0.9856711evidencecleaner02023-06-28T20:09:30ZDUMMY:X-ray dataRESULTStitle_228432CDR L2structure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T20:09:54ZL2kmab-08-06-1190060-g004.jpgf0004FIGfig_caption28439The superposition of CDR L2 backbones for all HC:LC pairs with light chains: (A) L1-39, (B) L3-11, (C) L3-20 and (D) L4-1.0.99593866experimental_methodcleaner02023-06-29T09:07:59ZMESH:superpositionstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T20:09:52ZL20.95072794complex_assemblycleaner02023-06-29T09:23:46ZGO:HC:LCstructure_elementSO:cleaner02023-06-28T16:14:08Zlight chains0.73722893mutantcleaner02023-06-28T21:05:50ZMESH:L1-390.7577236mutantcleaner02023-06-28T21:06:35ZMESH:L3-110.81435376mutantcleaner02023-06-28T21:07:16ZMESH:L3-200.76888275mutantcleaner02023-06-28T21:08:00ZMESH:L4-1RESULTSparagraph28562All four LCs have CDR L2 of the same length and canonical structure, L2-8-1 (Table 2). The CDR L2 conformations for each of the LCs paired with the 4 HCs are clustered more tightly than any of the other CDRs (rmsd values are in the range 0.09-0.16 Å), and all 4 sets have virtually the same conformation despite the sequence diversity of the loop. No significant conformation outliers are observed (Fig. 4). 0.47755066structure_elementcleaner02023-06-28T16:11:39ZSO:LCsstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T20:09:54ZL20.96698934mutantcleaner02023-06-28T21:32:49ZMESH:L2-8-1structure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T20:09:54ZL20.6896999structure_elementcleaner02023-06-28T16:11:39ZSO:LCs0.94972163structure_elementcleaner02023-06-28T16:11:33ZSO:HCs0.9946795structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.99516016evidencecleaner02023-06-28T20:10:11ZDUMMY:rmsd0.9969625structure_elementcleaner02023-06-29T08:58:52ZSO:loopRESULTStitle_228976CDR L3structure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T20:10:26ZL3kmab-08-06-1190060-g005.jpgf0005FIGfig_caption28983The superposition of CDR L3 backbones for all HC:LC pairs with light chains: (A) L1-39, (B) L3-11, (C) L3-20 and (D) L4-1.0.99597675experimental_methodcleaner02023-06-29T09:08:03ZMESH:superpositionstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T20:10:43ZL30.9498575complex_assemblycleaner02023-06-29T09:23:51ZGO:HC:LCstructure_elementSO:cleaner02023-06-28T16:14:08Zlight chains0.770716mutantcleaner02023-06-28T21:05:50ZMESH:L1-39mutantMESH:cleaner02023-06-28T21:06:35ZL3-110.7661131mutantcleaner02023-06-28T21:07:16ZMESH:L3-20mutantMESH:cleaner02023-06-28T21:08:01ZL4-1RESULTSparagraph29106As with CDR L2, all 4 LCs have CDR L3 of the same length and canonical structure, L3-9-cis7-1 (Table 2). The conformations of CDR L3 for L1-39, L3-11, and particularly for L320, are not as tightly clustered as those of L4-1 (Fig. 5). The slight conformational variability occurs in the region of amino acid residues 90-92, which is in contact with CDR H3. structure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T20:09:54ZL20.7866458structure_elementcleaner02023-06-28T16:11:39ZSO:LCsstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T20:11:10ZL30.9468112evidencecleaner02023-06-28T20:10:48ZDUMMY:structure0.9630782mutantcleaner02023-06-29T09:26:16ZMESH:L3-9-cis7-1structure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T20:11:25ZL3mutantMESH:cleaner02023-06-28T21:05:50ZL1-39mutantMESH:cleaner02023-06-28T21:06:35ZL3-110.7602481mutantcleaner02023-06-28T21:08:01ZMESH:L4-10.9677697residue_rangecleaner02023-06-29T09:18:44ZDUMMY:90-920.9951101structure_elementcleaner02023-06-28T16:25:22ZSO:CDR0.5268623structure_elementcleaner02023-06-28T19:05:15ZSO:H3RESULTStitle_229466CDR H3 conformational diversitystructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T19:05:15ZH3RESULTSparagraph29498As mentioned earlier, all 16 Fabs have the same CDR H3, for which the amino acid sequence is derived from the anti-CCL2 antibody CNTO 888. The loop and the 2 β-strands of the CDR H3 in this ‘parent’ structure are stabilized by H-bonds between the carbonyl oxygen and peptide nitrogen atoms in the 2 strands. An interesting feature of these CDR H3 structures is the presence of a water molecule that interacts with the peptide nitrogens and carbonyl oxygens near the bridging loop connecting the 2 β-strands. This water is present in both the bound (4DN4) and unbound (4DN3) forms of CNTO 888. The stem region of CDR H3 in the parental Fab is in a ‘kinked’ conformation, in which the indole nitrogen of Trp103 forms a hydrogen bond with the carbonyl oxygen of Leu100b. The carboxyl group of Asp101 forms a salt bridge with Arg94. These interactions are illustrated in Fig. S2.0.28241646structure_elementcleaner02023-06-28T15:58:47ZSO:Fabsstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T19:05:15ZH3protein_typeMESH:cleaner02023-06-28T15:45:48ZantibodychemicalCHEBI:cleaner02023-06-29T09:24:49ZCNTO 8880.9973061structure_elementcleaner02023-06-29T08:58:58ZSO:loop0.8799594structure_elementcleaner02023-06-29T08:59:01ZSO:β-strandsstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T19:05:15ZH30.99561113evidencecleaner02023-06-28T20:11:37ZDUMMY:structurebond_interactionMESH:melaniev@ebi.ac.uk2023-07-28T14:19:44ZH-bondsstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T19:05:15ZH30.9962054evidencecleaner02023-06-28T20:11:40ZDUMMY:structures0.99835867chemicalcleaner02023-06-29T09:24:59ZCHEBI:water0.99427104structure_elementcleaner02023-06-29T08:59:04ZSO:loop0.9647501structure_elementcleaner02023-06-29T08:59:06ZSO:β-strands0.99842465chemicalcleaner02023-06-29T09:25:01ZCHEBI:water0.99691653protein_statecleaner02023-06-29T09:29:33ZDUMMY:bound0.9966217protein_statecleaner02023-06-29T09:29:36ZDUMMY:unbound0.7319946chemicalcleaner02023-06-29T09:24:50ZCHEBI:CNTO 8880.9975282structure_elementcleaner02023-06-29T08:59:08ZSO:stem regionstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T19:05:15ZH30.14281757structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.8321708protein_statecleaner02023-06-29T07:42:05ZDUMMY:kinked0.99914753residue_name_numbercleaner02023-06-29T09:22:08ZDUMMY:Trp103bond_interactionMESH:melaniev@ebi.ac.uk2023-07-28T14:19:44Zhydrogen bond0.9991297residue_name_numbercleaner02023-06-29T09:22:11ZDUMMY:Leu100b0.9991573residue_name_numbercleaner02023-06-29T09:22:14ZDUMMY:Asp101bond_interactionMESH:melaniev@ebi.ac.uk2023-07-28T14:19:44Zsalt bridge0.999151residue_name_numbercleaner02023-06-29T09:22:16ZDUMMY:Arg94kmab-08-06-1190060-g006.jpgf0006FIGfig_caption30385Ribbon representations of (A) the superposition of all CDR H3s of the structures with complete backbone traces. (B) The CDR H3s rotated 90° about the y axis of the page. The structure of each CDR H3 is represented with a different color.0.99487334experimental_methodcleaner02023-06-28T20:12:34ZMESH:superpositionstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T20:13:06ZH3s0.99505204evidencecleaner02023-06-28T20:12:38ZDUMMY:structuresstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T20:12:58ZH3s0.9879155evidencecleaner02023-06-28T20:12:40ZDUMMY:structurestructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T19:05:15ZH3RESULTSparagraph30624Despite having the same amino acid sequence in all variants, CDR H3 has the highest degree of structural diversity and disorder of all of the CDRs in the experimental set. Three of the 21 Fab structures (including multiple copies in the asymmetric unit), H5-51:L3-11, H551:L3-20 and H3-23:L4-1 (one of the 2 Fabs), have missing (disordered) residues at the apex of the CDR loop. Another four of the Fabs, H3-23:L1-39, H3-53:L1-39, H3-53:L3-11 and H3-53:L4-1 have missing side-chain atoms. The variations in CDR H3 conformation are illustrated in Fig. 6 for the 18 Fab structures that have ordered backbone atoms. 0.9960975structure_elementcleaner02023-06-28T16:25:22ZSO:CDR0.5586551structure_elementcleaner02023-06-28T19:05:16ZSO:H30.99367166structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.18316415structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.99480397evidencecleaner02023-06-28T20:13:14ZDUMMY:structurescomplex_assemblyGO:cleaner02023-06-28T21:14:32ZH5-51:L3-11complex_assemblyGO:cleaner02023-06-28T21:34:46ZH551:L3-20complex_assemblyGO:cleaner02023-06-28T21:12:34ZH3-23:L4-10.31031048structure_elementcleaner02023-06-28T15:58:47ZSO:Fabs0.50068367protein_statecleaner02023-06-29T09:29:55ZDUMMY:missing0.76184237protein_statecleaner02023-06-29T09:29:58ZDUMMY:disordered0.9971243structure_elementcleaner02023-06-28T20:13:25ZSO:CDR loop0.26492673structure_elementcleaner02023-06-28T15:58:47ZSO:Fabscomplex_assemblyGO:cleaner02023-06-28T21:15:28ZH3-23:L1-39complex_assemblyGO:cleaner02023-06-28T21:13:04ZH3-53:L1-39complex_assemblyGO:cleaner02023-06-28T21:13:26ZH3-53:L3-11complex_assemblyGO:cleaner02023-06-28T21:18:20ZH3-53:L4-1structure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T19:05:16ZH30.18022169structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.9938689evidencecleaner02023-06-28T20:13:17ZDUMMY:structureskmab-08-06-1190060-g007.jpgf0007FIGfig_caption31240A comparison of representatives of the “kinked” and “extended” structures. (A) The “kinked” CDR H3 of H1-69:L3-11 with purple carbon atoms and yellow dashed lines connecting the H-bond pairs for Leu100b O and Trp103 NE1, Arg94 NE and Asp101 OD1, and Arg94 NH2 and Asp101 OD2. (B) The “extended” CDR H3 of H1-69:L3-20 with green carbon atoms and yellow dashed lines connecting the H-bond pairs for Asp101 OD1 and OD2 and Trp103 NE1.0.9908119protein_statecleaner02023-06-29T07:42:05ZDUMMY:kinked0.9959126protein_statecleaner02023-06-29T07:41:57ZDUMMY:extended0.99503183evidencecleaner02023-06-28T20:13:38ZDUMMY:structures0.9928145protein_statecleaner02023-06-29T07:42:05ZDUMMY:kinkedstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T19:05:16ZH30.81869656complex_assemblycleaner02023-06-28T21:36:23ZGO:H1-69:L3-110.999046residue_name_numbercleaner02023-06-29T09:22:22ZDUMMY:Leu100b0.99903286residue_name_numbercleaner02023-06-29T09:22:25ZDUMMY:Trp1030.99903893residue_name_numbercleaner02023-06-29T09:22:27ZDUMMY:Arg940.9990128residue_name_numbercleaner02023-06-29T09:22:32ZDUMMY:Asp1010.99903846residue_name_numbercleaner02023-06-29T09:22:35ZDUMMY:Arg940.9990054residue_name_numbercleaner02023-06-29T09:22:37ZDUMMY:Asp1010.99604356protein_statecleaner02023-06-29T07:41:57ZDUMMY:extendedstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T19:05:16ZH3complex_assemblyGO:cleaner02023-06-28T21:20:21ZH1-69:L3-200.9990422residue_name_numbercleaner02023-06-29T09:22:40ZDUMMY:Asp1010.9990233residue_name_numbercleaner02023-06-29T09:22:43ZDUMMY:Trp103RESULTSparagraph31688In 10 of the 18 Fab structures, H1-69:L1-39, H1-69:L3-11 (2 Fabs), H1-69:L4-1, H3-23:L3-11 (2 Fabs), H3-23:L3-20, H3-53:L3-11, H3-53:L3-20 and H5-51:L1-39, the CDRs have similar conformations to that found in 4DN3. The bases of these structures have the ‘kinked’ conformation with the H-bond between Trp103 and Leu100b. A representative CDR H3 structure for H1-69:L1-39 illustrating this is shown in Fig. 7A. The largest backbone conformational deviation for the set is at Tyr99, where the C=O is rotated by 90° relative to that observed in 4DN3. Also, it is worth noting that only one of these structures, H1-69:L4-1, has the conserved water molecule in CDR H3 observed in the 4DN3 and 4DN4 structures. In fact, it is the only Fab in the set that has a water molecule present at this site. The CDR H3 for this structure is shown in Fig. S3. 0.23736683structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.99279636evidencecleaner02023-06-28T20:14:41ZDUMMY:structurescomplex_assemblyGO:cleaner02023-06-28T21:37:02ZH1-69:L1-39complex_assemblyGO:cleaner02023-06-28T21:37:23ZH1-69:L3-11structure_elementSO:cleaner02023-06-28T15:58:47ZFabscomplex_assemblyGO:cleaner02023-06-28T21:37:43ZH1-69:L4-1complex_assemblyGO:cleaner02023-06-28T21:12:10ZH3-23:L3-11structure_elementSO:cleaner02023-06-28T15:58:47ZFabscomplex_assemblyGO:cleaner02023-06-28T21:38:15ZH3-23:L3-20complex_assemblyGO:cleaner02023-06-28T21:13:26ZH3-53:L3-11complex_assemblyGO:cleaner02023-06-28T21:13:47ZH3-53:L3-20complex_assemblyGO:cleaner02023-06-28T21:14:11ZH5-51:L1-390.99718827structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.9816032evidencecleaner02023-06-28T20:14:43ZDUMMY:structures0.755971protein_statecleaner02023-06-29T07:42:05ZDUMMY:kinked0.9990682residue_name_numbercleaner02023-06-29T09:22:48ZDUMMY:Trp1030.9990772residue_name_numbercleaner02023-06-29T09:22:50ZDUMMY:Leu100bstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T19:05:16ZH30.9840614evidencecleaner02023-06-28T20:14:46ZDUMMY:structurecomplex_assemblyGO:cleaner02023-06-28T21:39:19ZH1-69:L1-390.9991266residue_name_numbercleaner02023-06-29T09:22:55ZDUMMY:Tyr990.9504556evidencecleaner02023-06-28T20:14:58ZDUMMY:structurescomplex_assemblyGO:cleaner02023-06-28T21:39:43ZH1-69:L4-10.98424697protein_statecleaner02023-06-29T09:30:13ZDUMMY:conserved0.99795204chemicalcleaner02023-06-29T09:25:01ZCHEBI:waterstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T19:05:16ZH30.9916455evidencecleaner02023-06-28T20:15:00ZDUMMY:structures0.17939177structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.9982192chemicalcleaner02023-06-29T09:25:01ZCHEBI:waterstructure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T19:05:16ZH30.9559372evidencecleaner02023-06-28T20:15:04ZDUMMY:structureRESULTSparagraph32539The remaining 8 Fabs can be grouped into 5 different conformational classes. Three of the Fabs, H3-23:L1-39, H3-23:L4-1 and H3-53:L1-39, have distinctive conformations. The stem regions in these 3 cases are in the ‘kinked’ conformation consistent with that observed for 4DN3. The five remaining Fabs, H5-51:L4-1 (2 copies), H1-69:L3-20 (2 copies) and H3-53:L4-1, have 3 different CDR H3 conformations (Fig. S4). The stem regions of CDR H3 for the H5-51:L4-1 Fabs are in the ‘kinked’ conformation while, surprisingly, those of the H1-69:L3-20 pair and H3-53:L4-1 are in the ‘extended’ conformation (Fig. 7B).0.22445008structure_elementcleaner02023-06-28T15:58:47ZSO:Fabs0.25196815structure_elementcleaner02023-06-28T15:58:47ZSO:Fabscomplex_assemblyGO:cleaner02023-06-28T21:15:28ZH3-23:L1-39complex_assemblyGO:cleaner02023-06-28T21:12:34ZH3-23:L4-1complex_assemblyGO:cleaner02023-06-28T21:13:04ZH3-53:L1-390.9969319structure_elementcleaner02023-06-28T20:15:20ZSO:stem regions0.9289319protein_statecleaner02023-06-28T20:15:22ZDUMMY:kinked0.3686641structure_elementcleaner02023-06-28T15:58:47ZSO:Fabscomplex_assemblyGO:cleaner02023-06-28T21:40:48ZH5-51:L4-1complex_assemblyGO:cleaner02023-06-28T21:20:21ZH1-69:L3-20complex_assemblyGO:cleaner02023-06-28T21:18:21ZH3-53:L4-1structure_elementSO:cleaner02023-06-28T16:25:22ZCDRstructure_elementSO:cleaner02023-06-28T19:05:16ZH30.9966538structure_elementcleaner02023-06-29T08:59:58ZSO:stem regionsstructure_elementSO:cleaner02023-06-28T16:25:23ZCDRstructure_elementSO:cleaner02023-06-28T19:05:16ZH3complex_assemblyGO:cleaner02023-06-28T21:41:35ZH5-51:L4-1structure_elementSO:cleaner02023-06-28T15:58:47ZFabs0.97828144protein_statecleaner02023-06-28T20:15:25ZDUMMY:kinkedcomplex_assemblyGO:cleaner02023-06-28T21:20:21ZH1-69:L3-20complex_assemblyGO:cleaner02023-06-28T21:18:21ZH3-53:L4-10.99039197protein_statecleaner02023-06-29T07:41:56ZDUMMY:extendedRESULTStitle_233161VH:VL domain packingcomplex_assemblyGO:melaniev@ebi.ac.uk2023-07-06T15:18:10ZVH:VLRESULTSparagraph33182The VH and VL domains have a β-sandwich structure (also often referred as a Greek key motif) and each is composed of a 4-stranded and a 5-stranded antiparallel β-sheets. The two domains pack together such that the 5-stranded β-sheets, which have hydrophobic surfaces, interact with each other bringing the CDRs from both the VH and VL domains into close proximity. The domain packing of the variants was assessed by computing the domain interface interactions, the VH:VL tilt angles, the buried surface area and surface complementarity. The results of these analyses are shown in Tables 3, 4 and S2.0.9976998structure_elementcleaner02023-06-29T09:00:01ZSO:VH0.9978058structure_elementcleaner02023-06-28T19:10:07ZSO:VL0.9875603structure_elementcleaner02023-06-29T09:00:04ZSO:β-sandwich structure0.99606323structure_elementcleaner02023-06-29T09:00:07ZSO:Greek key motifstructure_elementSO:cleaner02023-06-29T09:00:27Z4-stranded and a 5-stranded antiparallel β-sheets0.99740285structure_elementcleaner02023-06-29T09:00:40ZSO:5-stranded β-sheets0.997398structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.99764204structure_elementcleaner02023-06-29T09:00:43ZSO:VH0.99792structure_elementcleaner02023-06-28T19:10:07ZSO:VLsiteSO:cleaner02023-06-29T09:11:11Zdomain interfacecomplex_assemblyGO:melaniev@ebi.ac.uk2023-07-06T15:18:10ZVH:VLevidenceDUMMY:cleaner02023-06-28T16:02:51Ztilt anglesRESULTStitle_333791VH:VL interface amino acid residue interactions0.98834515sitecleaner02023-06-28T20:18:37ZSO:VH:VL interfacekmab-08-06-1190060-g008.jpgf0008FIGfig_caption33839The conserved VH:VL interactions as viewed along the VH/VL axis. The VH residues are in blue, the VL residues are in orange.0.99727947protein_statecleaner02023-06-29T09:30:27ZDUMMY:conservedcomplex_assemblyGO:melaniev@ebi.ac.uk2023-07-06T15:18:10ZVH:VL0.79262924structure_elementcleaner02023-06-29T09:00:50ZSO:VHstructure_elementSO:cleaner02023-06-28T19:10:07ZVL0.99668914structure_elementcleaner02023-06-29T09:00:53ZSO:VH0.997074structure_elementcleaner02023-06-28T19:10:07ZSO:VLRESULTSparagraph33964The VH:VL interface is pseudosymmetric, and involves 2 stretches of the polypeptide chain from each domain, namely CDR3 and the framework region between CDRs 1 and 2. These stretches form antiparallel β-hairpins within the internal 5-stranded β-sheet. There are a few principal inter-domain interactions that are conserved not only in the experimental set of 16 Fabs, but in all human antibodies. They include: 1) a bidentate hydrogen bond between L-Gln38 and H-Gln39; 2) H-Leu45 in a hydrophobic pocket between L-Phe98, L-Tyr87 and L-Pro44; 3) L-Pro44 stacked against H-Trp103; and 4) L-Ala43 opposite the face of H-Tyr91 (Fig. 8). With the exception of L-Ala43, all other residues are conserved in human germlines. Position 43 may be alternatively occupied by Ser, Val or Pro (as in L4-1), but the hydrophobic interaction with H-Tyr91 is preserved. These core interactions provide enough stability to the VH:VL dimer so that additional VH-VL contacts can tolerate amino acid sequence variations in CDRs H3 and L3 that form part of the VH:VL interface. 0.99136263sitecleaner02023-06-28T20:18:37ZSO:VH:VL interface0.53883225protein_statecleaner02023-06-29T09:30:34ZDUMMY:pseudosymmetric0.9983753structure_elementcleaner02023-06-29T09:01:18ZSO:CDR30.9973372structure_elementcleaner02023-06-29T09:00:57ZSO:framework region0.99737406structure_elementcleaner02023-06-29T09:01:20ZSO:CDRs 1 and 20.993996structure_elementcleaner02023-06-29T09:01:01ZSO:antiparallel β-hairpins0.9913381structure_elementcleaner02023-06-29T09:01:03ZSO:5-stranded β-sheet0.95069045structure_elementcleaner02023-06-28T15:58:47ZSO:Fabs0.9924118speciescleaner02023-06-28T16:03:59ZMESH:human0.67610204protein_typecleaner02023-06-28T16:03:38ZMESH:antibodiesbond_interactionMESH:melaniev@ebi.ac.uk2023-07-28T14:19:44Zhydrogen bondstructure_elementSO:cleaner02023-06-28T20:19:40ZLresidue_name_numberDUMMY:cleaner02023-06-28T20:19:27ZGln380.8804055structure_elementcleaner02023-06-28T20:19:52ZSO:H0.84093565residue_name_numbercleaner02023-06-29T09:23:04ZDUMMY:Gln390.973559structure_elementcleaner02023-06-28T20:20:04ZSO:H0.692089residue_name_numbercleaner02023-06-29T09:23:07ZDUMMY:Leu450.99601036sitecleaner02023-06-28T20:18:42ZSO:hydrophobic pocketstructure_elementSO:cleaner02023-06-28T20:20:25ZL0.6455791residue_name_numbercleaner02023-06-28T20:20:11ZDUMMY:Phe98structure_elementSO:cleaner02023-06-28T20:20:47ZL0.75837576residue_name_numbercleaner02023-06-28T20:20:31ZDUMMY:Tyr87structure_elementSO:cleaner02023-06-28T20:21:11ZL0.7018562residue_name_numbercleaner02023-06-28T20:20:51ZDUMMY:Pro44structure_elementSO:cleaner02023-06-28T20:21:27ZL0.58544266residue_name_numbercleaner02023-06-28T20:21:15ZDUMMY:Pro440.9149529structure_elementcleaner02023-06-28T20:21:36ZSO:H0.9516314residue_name_numbercleaner02023-06-28T20:21:38ZDUMMY:Trp103structure_elementSO:cleaner02023-06-28T20:22:09ZLresidue_name_numberDUMMY:cleaner02023-06-28T20:21:58ZAla430.9800174structure_elementcleaner02023-06-28T20:22:22ZSO:H0.5058213residue_name_numbercleaner02023-06-29T09:23:13ZDUMMY:Tyr91structure_elementSO:cleaner02023-06-28T20:22:51ZLresidue_name_numberDUMMY:cleaner02023-06-28T20:22:39ZAla430.993141speciescleaner02023-06-28T16:03:59ZMESH:human0.9463635residue_numbercleaner02023-06-29T09:20:38ZDUMMY:430.98263526residue_namecleaner02023-06-29T09:12:33ZSO:Ser0.98448217residue_namecleaner02023-06-29T09:12:38ZSO:Val0.9818835residue_namecleaner02023-06-29T09:12:41ZSO:Pro0.914598mutantcleaner02023-06-28T21:08:01ZMESH:L4-1bond_interactionMESH:melaniev@ebi.ac.uk2023-07-28T14:19:44Zhydrophobic interaction0.9252548structure_elementcleaner02023-06-28T20:23:55ZSO:H0.58144456residue_name_numbercleaner02023-06-29T09:23:17ZDUMMY:Tyr910.91734785complex_assemblycleaner02023-06-29T09:23:56ZGO:VH:VL0.9946301oligomeric_statecleaner02023-06-29T09:23:22ZDUMMY:dimersiteSO:cleaner02023-06-28T20:23:28ZVH-VL contacts0.99753404structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.99678314structure_elementcleaner02023-06-28T19:05:16ZSO:H30.9974498structure_elementcleaner02023-06-29T09:01:23ZSO:L30.99011016sitecleaner02023-06-28T20:18:35ZSO:VH:VL interfaceRESULTSparagraph35026In total, about 20 residues are involved in the VH:VL interactions on each side (Fig. S5). Half of them are in the framework regions and those residues (except residue 61 in HC, which is actually in CDR2 in Kabat's definition) are conserved in the set of 16 Fabs. The side chain conformations of these conserved residues are also highly similar. One notable exception is H-Trp47, which exhibits 2 conformations of the indole ring. In most of the structures, it has the χ2 angle of ∼80°, while the ring is flipped over (χ2 = −100°) in H5-51:L3:11 and H5-51:L3-20. Interestingly, these are the only 2 structures with residues missing in CDR H3 because of disorder, although both structures are determined at high resolution and the rest of the structure is well defined. Apparently, residues flanking CDR H3 in the 2 VH:VL pairings are inconsistent with any stable conformation of CDR H3, which translates into a less restricted conformational space for some of them, including H-Trp47.residue_rangeDUMMY:cleaner02023-06-29T09:32:11Z20 residuescomplex_assemblyGO:melaniev@ebi.ac.uk2023-07-06T15:18:10ZVH:VL0.9973575structure_elementcleaner02023-06-29T09:01:26ZSO:framework regions0.91451746residue_numbercleaner02023-06-29T09:20:44ZDUMMY:610.99835485structure_elementcleaner02023-06-29T09:02:52ZSO:HC0.9977931structure_elementcleaner02023-06-29T09:02:23ZSO:CDR20.793795structure_elementcleaner02023-06-28T15:58:47ZSO:Fabsstructure_elementSO:cleaner02023-06-28T20:25:28ZHresidue_name_numberDUMMY:cleaner02023-06-28T20:25:18ZTrp470.9964148evidencecleaner02023-06-28T20:24:25ZDUMMY:structures0.9833544evidencecleaner02023-06-28T20:24:31ZDUMMY:χ20.9682395evidencecleaner02023-06-28T20:24:34ZDUMMY:χ2complex_assemblyGO:cleaner02023-06-28T21:42:48ZH5-51:L3:11complex_assemblyGO:cleaner02023-06-28T21:14:53ZH5-51:L3-200.99607605evidencecleaner02023-06-28T20:26:34ZDUMMY:structures0.98517585protein_statecleaner02023-06-29T09:31:44ZDUMMY:missingstructure_elementSO:cleaner02023-06-28T16:25:23ZCDRstructure_elementSO:cleaner02023-06-28T19:05:16ZH30.9922267evidencecleaner02023-06-28T20:24:37ZDUMMY:structures0.9962059evidencecleaner02023-06-28T20:24:39ZDUMMY:structurestructure_elementSO:cleaner02023-06-28T16:25:23ZCDRstructure_elementSO:cleaner02023-06-28T19:05:16ZH3complex_assemblyGO:melaniev@ebi.ac.uk2023-07-06T15:18:10ZVH:VL0.965915protein_statecleaner02023-06-29T09:31:42ZDUMMY:stablestructure_elementSO:cleaner02023-06-28T16:25:23ZCDRstructure_elementSO:cleaner02023-06-28T19:05:16ZH3structure_elementSO:cleaner02023-06-28T20:25:05ZHresidue_name_numberDUMMY:cleaner02023-06-28T20:24:55ZTrp47RESULTStitle_236020VH:VL tilt anglescomplex_assemblyGO:melaniev@ebi.ac.uk2023-07-06T15:18:10ZVH:VL0.78827584evidencecleaner02023-06-28T16:02:51ZDUMMY:tilt anglesRESULTSparagraph36038The relative orientation of VH and VL has been measured in a number of different ways. Presented here are the results of 2 different approaches for determining the orientation of one domain relative to the other.0.99675924structure_elementcleaner02023-06-29T09:02:25ZSO:VH0.99686structure_elementcleaner02023-06-28T19:10:07ZSO:VLRESULTSparagraph36251The first approach uses ABangles, the results of which are shown in Table S2. The four LCs all are classified as Type A because they have a proline at position 44, and the results for each orientation parameter are within the range of values of this type reported by Dunbar and co-workers. In fact, the parameter values for the set of 16 Fabs are in the middle of the distribution observed for 351 non-redundant antibody structures determined at 3.0 Å resolution or better. The only exception is HC1, which is shifted toward smaller angles with the mean value of 70.8° as compared to the distribution centered at 72° for the entire PDB. This probably reflects the invariance of CDR H3 in the current set as opposed to the CDR H3 diversity in the PDB.0.99128175experimental_methodcleaner02023-06-29T09:08:25ZMESH:ABangles0.52872384structure_elementcleaner02023-06-28T16:11:39ZSO:LCs0.9892429residue_namecleaner02023-06-29T09:12:47ZSO:proline0.96477294residue_numbercleaner02023-06-29T09:20:49ZDUMMY:440.76806116evidencecleaner02023-06-28T20:27:21ZDUMMY:orientation parameter0.14618859structure_elementcleaner02023-06-28T15:58:47ZSO:Fabsprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.9953317evidencecleaner02023-06-28T20:27:24ZDUMMY:structures0.99818486structure_elementcleaner02023-06-29T09:02:55ZSO:HC1structure_elementSO:cleaner02023-06-28T16:25:23ZCDRstructure_elementSO:cleaner02023-06-28T19:05:16ZH3structure_elementSO:cleaner02023-06-28T16:25:23ZCDRstructure_elementSO:cleaner02023-06-28T19:05:16ZH3RESULTSparagraph37007The second approach used for comparing tilt angles involved computing the difference in the tilt angles between all pairs of structures. For structures with 2 copies of the Fab in the asymmetric unit, only one structure was used. The differences between independent Fabs in the same structure are 4.9° for H1-69:L3-20, 1.6° for H1-69:L3-11, 1.4° for H3-23:L4-1, 3.3° for H3-23:L3-11, and 2.5° for H5-51:L4-1. With the exception of H1-69:L3-20, the angles are within the range of 2-3° as are observed in the identical structures in the PDB. In H1-69:L3-20, one of the Fabs is substantially disordered so that part of CDR H2 (the outer β-strand, residues 55-60) is completely missing. This kind of disorder may compromise the integrity of the VH domain and its interaction with the VL. Indeed, this Fab has the largest twist angle HC2 within the experimental set that exceeds the mean value by 2.5 standard deviations (Table S2).0.99319816evidencecleaner02023-06-28T16:02:51ZDUMMY:tilt angles0.73657733evidencecleaner02023-06-28T20:27:39ZDUMMY:difference0.98982793evidencecleaner02023-06-28T16:02:51ZDUMMY:tilt angles0.9951414evidencecleaner02023-06-28T20:27:45ZDUMMY:structures0.9731836evidencecleaner02023-06-28T20:27:48ZDUMMY:structuresstructure_elementSO:cleaner02023-06-28T15:59:05ZFab0.9942374evidencecleaner02023-06-28T20:27:51ZDUMMY:structure0.14642434structure_elementcleaner02023-06-28T15:58:47ZSO:Fabs0.9922145evidencecleaner02023-06-28T20:27:53ZDUMMY:structure0.730512complex_assemblycleaner02023-06-28T21:20:21ZGO:H1-69:L3-200.7319917complex_assemblycleaner02023-06-28T21:43:25ZGO:H1-69:L3-11complex_assemblyGO:cleaner02023-06-28T21:12:34ZH3-23:L4-10.678943complex_assemblycleaner02023-06-28T21:12:10ZGO:H3-23:L3-11complex_assemblyGO:cleaner02023-06-28T21:44:05ZH5-51:L4-1complex_assemblyGO:cleaner02023-06-28T21:20:21ZH1-69:L3-200.99507105evidencecleaner02023-06-28T20:28:06ZDUMMY:structures0.92975956complex_assemblycleaner02023-06-28T21:20:21ZGO:H1-69:L3-200.19363964structure_elementcleaner02023-06-28T15:58:47ZSO:Fabs0.76591015protein_statecleaner02023-06-29T09:33:14ZDUMMY:disorderedstructure_elementSO:cleaner02023-06-28T16:25:23ZCDRstructure_elementSO:cleaner02023-06-28T16:26:21ZH20.948555structure_elementcleaner02023-06-29T09:03:03ZSO:β-strand0.9751329residue_rangecleaner02023-06-29T09:18:50ZDUMMY:55-600.9974107structure_elementcleaner02023-06-29T09:02:36ZSO:VH0.9971015structure_elementcleaner02023-06-28T19:10:07ZSO:VL0.25028703structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.9712751evidencecleaner02023-06-28T20:28:11ZDUMMY:twist anglestructure_elementSO:cleaner02023-06-28T20:28:21ZHC2kmab-08-06-1190060-g009.jpgf0009FIGfig_caption37943An illustration of the difference in tilt angle for 2 pairs of variants by the superposition of the VH domains of (A) H1-69:L3-20 on that of H5-51:L1-39 (the VL domain is off by a rigid-body roatation of 10.5°) and (B) H1-69:L4-1 on that of H5-51:L1-39 (the VL domain is off by a rigid-body roatation of 1.6°).0.99561775experimental_methodcleaner02023-06-28T20:28:35ZMESH:superposition0.99801207structure_elementcleaner02023-06-29T09:03:16ZSO:VH0.93959236complex_assemblycleaner02023-06-28T21:20:21ZGO:H1-69:L3-200.9412726complex_assemblycleaner02023-06-28T21:14:11ZGO:H5-51:L1-390.9981108structure_elementcleaner02023-06-28T19:10:07ZSO:VLcomplex_assemblyGO:cleaner02023-06-28T21:45:10ZH1-69:L4-10.9760524complex_assemblycleaner02023-06-28T21:14:11ZGO:H5-51:L1-390.9981937structure_elementcleaner02023-06-28T19:10:07ZSO:VLt0003.xmlt0003TABLEtable_caption38256Differences in VH:VL tilt angles.complex_assemblyGO:melaniev@ebi.ac.uk2023-07-06T15:18:10ZVH:VLevidenceDUMMY:cleaner02023-06-28T16:02:51Ztilt anglest0003.xmlt0003TABLEtable<?xml version="1.0" encoding="UTF-8"?>
<table frame="hsides" rules="groups"><colgroup><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/></colgroup><tbody><tr><td align="left"> <hr/></td><td align="center">H1-69:L1-39<hr/></td><td align="center">H1-69:L3-11<hr/></td><td align="center">H1-69:L3-20<hr/></td><td align="center">H1-69:L4-1<hr/></td><td align="center">H3-23:L1-39<hr/></td><td align="center">H3-23:L3-11<hr/></td><td align="center">H3-23:L3-20<hr/></td><td align="center">H3-23:L4-1<hr/></td><td align="center">H3-53:L1-39<hr/></td><td align="center">H3-53:L3-11<hr/></td><td align="center">H3-53:L3-20<hr/></td><td align="center">H3-53:L4-1<hr/></td><td align="center">H5-51:L1-39<hr/></td><td align="center">H5-51:L3-11<hr/></td><td align="center">H5-51:L3-20<hr/></td><td align="center">H5-51:L4-1<hr/></td></tr><tr><td align="center">H1-69:L1-39</td><td align="center"><bold>0</bold></td><td align="char" char="."><bold>2.1</bold></td><td align="center"><bold>8.9</bold></td><td align="char" char="."><bold>1.1</bold></td><td align="char" char="."><bold>4.2</bold></td><td align="center"><bold>3.0</bold></td><td align="center"><bold>9.5</bold></td><td align="char" char="."><bold>1.5</bold></td><td align="center"><bold>3.3</bold></td><td align="char" char="."><bold>3.6</bold></td><td align="char" char="."><bold>3.1</bold></td><td align="char" char="."><bold>1.6</bold></td><td align="center"><bold>1.8</bold></td><td align="char" char="."><bold>2.9</bold></td><td align="char" char="."><bold>2.4</bold></td><td align="char" char="."><bold>5.2</bold></td></tr><tr><td align="center">H1-69:L3-11</td><td align="center"> </td><td align="center"><bold>0</bold></td><td align="center"><bold>7.3</bold></td><td align="char" char="."><bold>2.9</bold></td><td align="char" char="."><bold>2.5</bold></td><td align="char" char="."><bold>2.0</bold></td><td align="center"><bold>8.4</bold></td><td align="char" char="."><bold>1.3</bold></td><td align="center"><bold>2.6</bold></td><td align="char" char="."><bold>2.9</bold></td><td align="char" char="."><bold>3.2</bold></td><td align="char" char="."><bold>1.8</bold></td><td align="char" char="."><bold>3.9</bold></td><td align="char" char="."><bold>4.6</bold></td><td align="char" char="."><bold>4.4</bold></td><td align="char" char="."><bold>5.0</bold></td></tr><tr><td align="center">H1-69:L3-20</td><td align="center"> </td><td align="center"> </td><td align="center"><bold>0</bold></td><td align="center"><bold>9.2</bold></td><td align="center"><bold>5.0</bold></td><td align="center"><bold>8.7</bold></td><td align="center"><bold>7.4</bold></td><td align="center"><bold>7.6</bold></td><td align="center"><bold>8.9</bold></td><td align="center"><bold>8.6</bold></td><td align="center"><bold>9.4</bold></td><td align="center"><bold>7.9</bold></td><td align="center"><bold>10.5</bold></td><td align="center"><bold>10.1</bold></td><td align="center"><bold>11.0</bold></td><td align="center"><bold>9.7</bold></td></tr><tr><td align="center">H1-69:L4-1</td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"><bold>0</bold></td><td align="char" char="."><bold>4.6</bold></td><td align="char" char="."><bold>3.9</bold></td><td align="center"><bold>10.1</bold></td><td align="char" char="."><bold>1.8</bold></td><td align="char" char="."><bold>4.4</bold></td><td align="char" char="."><bold>4.7</bold></td><td align="char" char="."><bold>4.1</bold></td><td align="char" char="."><bold>2.3</bold></td><td align="char" char="."><bold>1.6</bold></td><td align="char" char="."><bold>2.5</bold></td><td align="char" char="."><bold>2.3</bold></td><td align="char" char="."><bold>6.2</bold></td></tr><tr><td align="center">H3-23:L1-39</td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"><bold>0</bold></td><td align="center"><bold>4.0</bold></td><td align="center"><bold>8.0</bold></td><td align="char" char="."><bold>2.8</bold></td><td align="char" char="."><bold>4.8</bold></td><td align="char" char="."><bold>4.8</bold></td><td align="char" char="."><bold>5.4</bold></td><td align="char" char="."><bold>3.6</bold></td><td align="char" char="."><bold>6.0</bold></td><td align="char" char="."><bold>6.2</bold></td><td align="char" char="."><bold>6.6</bold></td><td align="char" char="."><bold>6.6</bold></td></tr><tr><td align="center">H3-23:L3-11</td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"><bold>0</bold></td><td align="center"><bold>9.3</bold></td><td align="char" char="."><bold>3.0</bold></td><td align="char" char="."><bold>2.1</bold></td><td align="char" char="."><bold>2.9</bold></td><td align="char" char="."><bold>3.3</bold></td><td align="char" char="."><bold>3.3</bold></td><td align="char" char="."><bold>4.6</bold></td><td align="char" char="."><bold>5.8</bold></td><td align="char" char="."><bold>5.0</bold></td><td align="char" char="."><bold>5.2</bold></td></tr><tr><td align="center">H3-23:L3-20</td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"><bold>0</bold></td><td align="center"><bold>8.9</bold></td><td align="center"><bold>7.9</bold></td><td align="center"><bold>7.0</bold></td><td align="center"><bold>7.6</bold></td><td align="center"><bold>7.9</bold></td><td align="center"><bold>10.5</bold></td><td align="center"><bold>9.7</bold></td><td align="center"><bold>10.7</bold></td><td align="center"><bold>6.2</bold></td></tr><tr><td align="center">H3-23:L4-1</td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"><bold>0</bold></td><td align="char" char="."><bold>3.6</bold></td><td align="char" char="."><bold>3.8</bold></td><td align="char" char="."><bold>3.7</bold></td><td align="char" char="."><bold>1.5</bold></td><td align="char" char="."><bold>3.2</bold></td><td align="char" char="."><bold>3.7</bold></td><td align="char" char="."><bold>3.8</bold></td><td align="char" char="."><bold>5.6</bold></td></tr><tr><td align="center">H3-53:L1-39</td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="char" char="."><bold>0</bold></td><td align="char" char="."><bold>1.0</bold></td><td align="char" char="."><bold>1.6</bold></td><td align="char" char="."><bold>2.9</bold></td><td align="char" char="."><bold>4.6</bold></td><td align="char" char="."><bold>5.3</bold></td><td align="char" char="."><bold>4.8</bold></td><td align="char" char="."><bold>3.1</bold></td></tr><tr><td align="center">H3-53:L3-11</td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"><bold>0</bold></td><td align="char" char="."><bold>1.3</bold></td><td align="char" char="."><bold>2.9</bold></td><td align="char" char="."><bold>4.8</bold></td><td align="char" char="."><bold>5.2</bold></td><td align="char" char="."><bold>5.0</bold></td><td align="char" char="."><bold>2.3</bold></td></tr><tr><td align="center">H3-53:L3-20</td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="char" char="."><bold>0</bold></td><td align="char" char="."><bold>2.5</bold></td><td align="char" char="."><bold>3.8</bold></td><td align="char" char="."><bold>4.2</bold></td><td align="char" char="."><bold>3.9</bold></td><td align="char" char="."><bold>2.2</bold></td></tr><tr><td align="center">H3-53:L4-1</td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"><bold>0</bold></td><td align="char" char="."><bold>2.9</bold></td><td align="char" char="."><bold>3.0</bold></td><td align="char" char="."><bold>3.3</bold></td><td align="char" char="."><bold>4.2</bold></td></tr><tr><td align="center">H5-51:L1-39</td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"><bold>0</bold></td><td align="char" char="."><bold>1.9</bold></td><td align="char" char="."><bold>0.6</bold></td><td align="char" char="."><bold>5.8</bold></td></tr><tr><td align="center">H5-51:L3-11</td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"><bold>0</bold></td><td align="center"><bold>1.9</bold></td><td align="char" char="."><bold>5.7</bold></td></tr><tr><td align="center">H5-51:L3-20</td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"><bold>0</bold></td><td align="char" char="."><bold>5.8</bold></td></tr><tr><td align="center">H5-51:L4-1</td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"> </td><td align="center"><bold>0</bold></td></tr></tbody></table>
38290 H1-69:L1-39 H1-69:L3-11 H1-69:L3-20 H1-69:L4-1 H3-23:L1-39 H3-23:L3-11 H3-23:L3-20 H3-23:L4-1 H3-53:L1-39 H3-53:L3-11 H3-53:L3-20 H3-53:L4-1 H5-51:L1-39 H5-51:L3-11 H5-51:L3-20 H5-51:L4-1 H1-69:L1-39 0 2.1 8.9 1.1 4.2 3.0 9.5 1.5 3.3 3.6 3.1 1.6 1.8 2.9 2.4 5.2 H1-69:L3-11 0 7.3 2.9 2.5 2.0 8.4 1.3 2.6 2.9 3.2 1.8 3.9 4.6 4.4 5.0 H1-69:L3-20 0 9.2 5.0 8.7 7.4 7.6 8.9 8.6 9.4 7.9 10.5 10.1 11.0 9.7 H1-69:L4-1 0 4.6 3.9 10.1 1.8 4.4 4.7 4.1 2.3 1.6 2.5 2.3 6.2 H3-23:L1-39 0 4.0 8.0 2.8 4.8 4.8 5.4 3.6 6.0 6.2 6.6 6.6 H3-23:L3-11 0 9.3 3.0 2.1 2.9 3.3 3.3 4.6 5.8 5.0 5.2 H3-23:L3-20 0 8.9 7.9 7.0 7.6 7.9 10.5 9.7 10.7 6.2 H3-23:L4-1 0 3.6 3.8 3.7 1.5 3.2 3.7 3.8 5.6 H3-53:L1-39 0 1.0 1.6 2.9 4.6 5.3 4.8 3.1 H3-53:L3-11 0 1.3 2.9 4.8 5.2 5.0 2.3 H3-53:L3-20 0 2.5 3.8 4.2 3.9 2.2 H3-53:L4-1 0 2.9 3.0 3.3 4.2 H5-51:L1-39 0 1.9 0.6 5.8 H5-51:L3-11 0 1.9 5.7 H5-51:L3-20 0 5.8 H5-51:L4-1 0 RESULTSparagraph39582The differences in the tilt angle are shown for all pairs of V regions in Table 3. They range from 0.6° to 11.0°. The smallest differences in the tilt angle are between the Fabs in isomorphous crystal forms. The largest deviations in the tilt angle, up to 11.0°, are found for 2 structures, H1-69:L3-20 and H3-23:L3-20, that stand out from the other Fabs. One of the 2 structures, H1-69:L3-20, has its CDR H3 in the ‘extended’ conformation; the other structure has it in the ‘kinked’ conformation. Two examples illustrating large (10.5°) and small (1.6°) differences in the tilt angles are shown in Fig. 9. 0.8249283evidencecleaner02023-06-28T20:29:14ZDUMMY:differences0.98597944evidencecleaner02023-06-28T20:29:17ZDUMMY:tilt angle0.99408334structure_elementcleaner02023-06-28T19:05:51ZSO:V regions0.98391706evidencecleaner02023-06-28T20:29:28ZDUMMY:tilt angle0.26356268structure_elementcleaner02023-06-28T15:58:47ZSO:Fabs0.885286evidencecleaner02023-06-28T20:29:31ZDUMMY:crystal forms0.9822982evidencecleaner02023-06-28T20:29:34ZDUMMY:tilt angle0.98341817evidencecleaner02023-06-28T20:29:37ZDUMMY:structurescomplex_assemblyGO:cleaner02023-06-28T21:20:21ZH1-69:L3-20complex_assemblyGO:cleaner02023-06-28T21:46:07ZH3-23:L3-200.33730918structure_elementcleaner02023-06-28T15:58:47ZSO:Fabs0.9868803evidencecleaner02023-06-28T20:29:40ZDUMMY:structurescomplex_assemblyGO:cleaner02023-06-28T21:20:21ZH1-69:L3-200.9975684structure_elementcleaner02023-06-28T16:25:23ZSO:CDR0.64334047structure_elementcleaner02023-06-28T19:05:17ZSO:H3protein_stateDUMMY:cleaner02023-06-29T07:41:57Zextendedprotein_stateDUMMY:cleaner02023-06-29T07:42:05Zkinked0.97952867evidencecleaner02023-06-28T16:02:51ZDUMMY:tilt anglesRESULTStitle_240207VH:VL buried surface area and complementaritycomplex_assemblyGO:melaniev@ebi.ac.uk2023-07-06T15:18:10ZVH:VLt0004.xmlt0004TABLEtable_caption40253VH:VL surface areas and surface complementarity.complex_assemblyGO:melaniev@ebi.ac.uk2023-07-06T15:18:10ZVH:VLt0004.xmlt0004TABLEtable<?xml version="1.0" encoding="UTF-8"?>
<table frame="hsides" rules="groups"><colgroup><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/></colgroup><thead><tr><th align="left">Chain Pairs</th><th align="center">PDB</th><th align="center">Contact surfaceVH (Å<sup>2</sup>)</th><th align="center">Contact surfaceVL (Å<sup>2</sup>)</th><th align="center">Interface(Å<sup>2</sup>)</th><th align="center">Surface complementarity</th></tr></thead><tbody><tr><td align="center"><bold>H1-69:L1-39</bold></td><td align="center">5I15</td><td align="center">727</td><td align="center">771</td><td align="center">749</td><td align="char" char=".">0.743</td></tr><tr><td align="center"><bold>H1-69:L3-11</bold></td><td align="center">5I16</td><td align="center">802</td><td align="center">870</td><td align="center">836</td><td align="char" char=".">0.762</td></tr><tr><td align="center"><bold>H1-69:L3-20</bold></td><td align="center">5I17</td><td align="center">713</td><td align="center">736</td><td align="center">725</td><td align="char" char=".">0.723</td></tr><tr><td align="center"><bold>H1-69:L4-1</bold></td><td align="center">5I18</td><td align="center">729</td><td align="center">736</td><td align="center">733</td><td align="char" char=".">0.734</td></tr><tr><td align="center">H3-23:L1-39<xref ref-type="bibr" rid="cit0001"><sup><bold>1</bold></sup></xref></td><td align="center">5I19</td><td align="center">795</td><td align="center">817</td><td align="center">806</td><td align="char" char=".">0.722</td></tr><tr><td align="center"><bold>H3-23:L3-11</bold></td><td align="center">5I1A</td><td align="center">822</td><td align="center">834</td><td align="center">828</td><td align="char" char=".">0.725</td></tr><tr><td align="center"><bold>H3-23:L3-20</bold></td><td align="center">5I1C</td><td align="center">670</td><td align="center">698</td><td align="center">684</td><td align="char" char=".">0.676</td></tr><tr><td align="center"><bold>H3-23:L4-1</bold></td><td align="center">5I1D</td><td align="center">743</td><td align="center">770</td><td align="center">757</td><td align="char" char=".">0.708</td></tr><tr><td align="center">H3-53:L1-39<xref ref-type="bibr" rid="cit0001"><sup><bold>1</bold></sup></xref></td><td align="center">5I1E</td><td align="center">698</td><td align="center">719</td><td align="center">709</td><td align="center">0.712</td></tr><tr><td align="center">H3-53:L3-11<xref ref-type="bibr" rid="cit0001"><sup><bold>1</bold></sup></xref></td><td align="center">5I1G</td><td align="center">747</td><td align="center">758</td><td align="center">753</td><td align="center">0.690</td></tr><tr><td align="center"><bold>H3-53:L3-20</bold></td><td align="center">5I1H</td><td align="center">743</td><td align="center">735</td><td align="center">739</td><td align="char" char=".">0.687</td></tr><tr><td align="center"><bold>H3-53:L4-1</bold></td><td align="center">5I1I</td><td align="center">689</td><td align="center">693</td><td align="center">691</td><td align="char" char=".">0.711</td></tr><tr><td align="center"><bold>H5-51:L1-39</bold></td><td align="center">4KMT</td><td align="center">761</td><td align="center">808</td><td align="center">785</td><td align="char" char=".">0.728</td></tr><tr><td align="center">H5-51:L3-11<xref ref-type="bibr" rid="cit0002"><sup><bold>2</bold></sup></xref></td><td align="center">5I1J</td><td align="center">648</td><td align="center">714</td><td align="center">681</td><td align="center">0.717</td></tr><tr><td align="center">H5-51:L3-20<xref ref-type="bibr" rid="cit0002"><sup><bold>2</bold></sup></xref></td><td align="center">5I1K</td><td align="center">622</td><td align="center">643</td><td align="center">633</td><td align="center">0.740</td></tr><tr><td align="center"><bold>H5-51:L4-1</bold></td><td align="center">5I1L</td><td align="center">790</td><td align="center">792</td><td align="center">791</td><td align="char" char=".">0.704</td></tr></tbody></table>
40302Chain Pairs PDB Contact surfaceVH (Å2) Contact surfaceVL (Å2) Interface(Å2) Surface complementarity H1-69:L1-39 5I15 727 771 749 0.743 H1-69:L3-11 5I16 802 870 836 0.762 H1-69:L3-20 5I17 713 736 725 0.723 H1-69:L4-1 5I18 729 736 733 0.734 H3-23:L1-39 5I19 795 817 806 0.722 H3-23:L3-11 5I1A 822 834 828 0.725 H3-23:L3-20 5I1C 670 698 684 0.676 H3-23:L4-1 5I1D 743 770 757 0.708 H3-53:L1-39 5I1E 698 719 709 0.712 H3-53:L3-11 5I1G 747 758 753 0.690 H3-53:L3-20 5I1H 743 735 739 0.687 H3-53:L4-1 5I1I 689 693 691 0.711 H5-51:L1-39 4KMT 761 808 785 0.728 H5-51:L3-11 5I1J 648 714 681 0.717 H5-51:L3-20 5I1K 622 643 633 0.740 H5-51:L4-1 5I1L 790 792 791 0.704 t0004.xmlt0004TABLEtable_footnote40999Some side chain atoms in CDR H3 are missing.structure_elementSO:cleaner02023-06-28T16:25:23ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH3t0004.xmlt0004TABLEtable_footnote41044Residues in CDR H3 are missing: YGE in H5-51:L3-11, GIY in H5-51:L3-20.structure_elementSO:cleaner02023-06-28T16:25:23ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH30.9964439structure_elementcleaner02023-06-29T07:43:58ZSO:YGEcomplex_assemblyGO:cleaner02023-06-28T21:14:32ZH5-51:L3-110.9953916structure_elementcleaner02023-06-29T07:44:08ZSO:GIYcomplex_assemblyGO:cleaner02023-06-28T21:14:53ZH5-51:L3-20RESULTSparagraph41116The results of the PISA contact surface calculation and surface complementarity calculation are shown in Table 4. The interface areas are calculated as the average of the VH and VL contact surfaces. Six of the 16 structures have CDR H3 side chains or complete residues missing, and therefore their interfaces are much smaller than in the other 10 structures with complete CDRs (the results are provided for all Fabs for completeness). Among the complete structures, the interface areas range from 684 to 836 Å2. Interestingly, the 2 structures that have the largest tilt angle differences with the other variants, H3-23:L3-20 and H1-69:L3-20, have the smallest VH:VL interfaces, 684 and 725 Å2, respectively. H3-23:L3-20 is also unique in that it has the lowest value (0.676) of surface complementarity. 0.99178493experimental_methodcleaner02023-06-28T20:32:34ZMESH:PISA0.9650426experimental_methodcleaner02023-06-28T20:32:42ZMESH:contact surface calculation0.96886826experimental_methodcleaner02023-06-28T20:32:54ZMESH:surface complementarity calculation0.5817277sitecleaner02023-06-29T08:16:00ZSO:interfacesiteSO:cleaner02023-06-29T08:16:44ZVH and VL contact surfaces0.992105evidencecleaner02023-06-29T08:41:06ZDUMMY:structuresstructure_elementSO:cleaner02023-06-28T16:25:23ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH30.6253964protein_statecleaner02023-06-29T09:33:23ZDUMMY:missing0.8131186sitecleaner02023-06-29T09:11:20ZSO:interfaces0.9851922evidencecleaner02023-06-29T08:41:10ZDUMMY:structures0.9952638protein_statecleaner02023-06-29T09:33:29ZDUMMY:complete0.9972295structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.28258172structure_elementcleaner02023-06-28T15:58:47ZSO:Fabs0.9950252protein_statecleaner02023-06-29T09:33:35ZDUMMY:complete0.9946089evidencecleaner02023-06-29T08:41:13ZDUMMY:structures0.49001282sitecleaner02023-06-29T08:41:22ZSO:interface0.9873541evidencecleaner02023-06-29T08:41:29ZDUMMY:structures0.9782131evidencecleaner02023-06-29T08:41:32ZDUMMY:tilt angle differences0.9572224complex_assemblycleaner02023-06-29T07:44:30ZGO:H3-23:L3-200.9546193complex_assemblycleaner02023-06-28T21:20:21ZGO:H1-69:L3-200.87709194sitecleaner02023-06-29T09:11:25ZSO:VH:VL interfaces0.98440266complex_assemblycleaner02023-06-29T07:45:05ZGO:H3-23:L3-20evidenceDUMMY:cleaner02023-06-29T08:42:18Zsurface complementarityRESULTStitle_341927Stability of germline pairingst0005.xmlt0005TABLEtable_caption41958Melting temperatures for the 16 Fabs.0.99553233evidencecleaner02023-06-29T08:42:23ZDUMMY:Melting temperatures0.5436273structure_elementcleaner02023-06-28T15:58:47ZSO:Fabst0005.xmlt0005TABLEtable<?xml version="1.0" encoding="UTF-8"?>
<table frame="hsides" rules="groups"><colgroup><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/><col width="86.4pt" align="left"/></colgroup><thead><tr><th align="left"> </th><th align="center">L3-20</th><th align="center">L4-1</th><th align="center">L3-11</th><th align="center">L1-39</th><th align="center">HC average</th></tr></thead><tbody><tr><td align="char" char=".">H1-69</td><td align="char" char=".">73.6</td><td align="char" char=".">74.8</td><td align="char" char=".">75.6</td><td align="char" char=".">80.3</td><td align="char" char=".">76.1</td></tr><tr><td align="left">H3-23</td><td align="char" char=".">74.8</td><td align="char" char=".">75.2</td><td align="char" char=".">4.8</td><td align="char" char=".">81.5</td><td align="char" char=".">76.6</td></tr><tr><td align="left">H3-53</td><td align="char" char=".">68.4</td><td align="char" char=".">68.0</td><td align="char" char=".">71.5</td><td align="char" char=".">73.9</td><td align="char" char=".">70.5</td></tr><tr><td align="left">H5-51</td><td align="char" char=".">68.4</td><td align="char" char=".">68.4</td><td align="char" char=".">71.9</td><td align="char" char=".">77.0</td><td align="char" char=".">71.4</td></tr><tr><td align="left">LC average</td><td align="center">71.3</td><td align="char" char=".">71.6</td><td align="char" char=".">73.5</td><td align="char" char=".">78.2</td><td align="center"> </td></tr></tbody></table>
41996 L3-20 L4-1 L3-11 L1-39 HC average H1-69 73.6 74.8 75.6 80.3 76.1 H3-23 74.8 75.2 4.8 81.5 76.6 H3-53 68.4 68.0 71.5 73.9 70.5 H5-51 68.4 68.4 71.9 77.0 71.4 LC average 71.3 71.6 73.5 78.2 t0005.xmlt0005TABLEtable_footnote42203Colors: blue (Tm < 70°C), green (70°C < Tm < 73°C), yellow (73°C < Tm < 78°C), orange (Tm > 78°C).0.78301543evidencecleaner02023-06-29T08:42:28ZDUMMY:Tm0.8056168evidencecleaner02023-06-29T08:42:31ZDUMMY:Tm0.90112174evidencecleaner02023-06-29T08:42:33ZDUMMY:Tm0.54672956evidencecleaner02023-06-29T08:42:36ZDUMMY:TmRESULTSparagraph42308Melting temperatures (Tm) were measured for all Fabs using differential scanning calorimetry (Table 5). It appears that for each given LC, the Fabs with germlines H1-69 and H3-23 are substantially more stable than those with germlines H3-53 and H5-51. In addition, L1-39 provides a much higher degree of stabilization than the other 3 LC germlines when combined with any of the HCs. As a result, the Tm for pairs H1-69:L1-39 and H3-23:L1-39 is 12-13° higher than for pairs H3-53:L3-20, H3-53:L4-1, H5-51:L3-20 and H5-51:L4-1. 0.9944316evidencecleaner02023-06-29T08:42:40ZDUMMY:Melting temperatures0.9929166evidencecleaner02023-06-29T08:42:43ZDUMMY:Tm0.22407825structure_elementcleaner02023-06-28T15:58:47ZSO:Fabs0.9954069experimental_methodcleaner02023-06-29T09:08:34ZMESH:differential scanning calorimetry0.93906766structure_elementcleaner02023-06-28T16:24:55ZSO:LC0.17053662structure_elementcleaner02023-06-28T15:58:47ZSO:FabsmutantMESH:cleaner02023-06-28T21:03:20ZH1-690.49335834mutantcleaner02023-06-28T21:04:18ZMESH:H3-230.74506783protein_statecleaner02023-06-29T09:33:43ZDUMMY:stablemutantMESH:cleaner02023-06-28T21:08:33ZH3-53mutantMESH:cleaner02023-06-28T21:05:06ZH5-510.8035386mutantcleaner02023-06-28T21:05:50ZMESH:L1-390.69415265structure_elementcleaner02023-06-28T16:24:55ZSO:LC0.99619627structure_elementcleaner02023-06-28T16:11:33ZSO:HCs0.9950289evidencecleaner02023-06-29T08:42:56ZDUMMY:Tmcomplex_assemblyGO:cleaner02023-06-29T08:43:14ZH1-69:L1-39complex_assemblyGO:cleaner02023-06-28T21:15:28ZH3-23:L1-39complex_assemblyGO:cleaner02023-06-28T21:13:47ZH3-53:L3-20complex_assemblyGO:cleaner02023-06-28T21:18:21ZH3-53:L4-1complex_assemblyGO:cleaner02023-06-28T21:14:53ZH5-51:L3-20complex_assemblyGO:cleaner02023-06-29T07:47:40ZH5-51:L4-1RESULTSparagraph42838These findings correlate well with the degree of conformational disorder observed in the crystal structures. Parts of CDR H3 main chain are completely disordered, and were not modeled in Fabs H5-51:L3-20 and H5-51:L3-11 that have the lowest Tms in the set. No electron density is observed for a number of side chains in CDRs H3 and L3 in all Fabs with germline H3-53, which indicates loose packing of the variable domains. All those molecules are relatively unstable, as is reflected in their low Tms.0.9975747evidencecleaner02023-06-28T19:16:53ZDUMMY:crystal structures0.997758structure_elementcleaner02023-06-28T16:25:23ZSO:CDR0.84807676structure_elementcleaner02023-06-28T19:05:17ZSO:H30.68790114protein_statecleaner02023-06-29T09:33:49ZDUMMY:disorderedstructure_elementSO:cleaner02023-06-28T15:58:47ZFabscomplex_assemblyGO:cleaner02023-06-28T21:14:53ZH5-51:L3-20complex_assemblyGO:cleaner02023-06-28T21:14:32ZH5-51:L3-110.6941904evidencecleaner02023-06-29T08:43:41ZDUMMY:Tms0.9930948evidencecleaner02023-06-29T08:43:46ZDUMMY:electron density0.9978542structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.9923321structure_elementcleaner02023-06-28T19:05:17ZSO:H30.99722135structure_elementcleaner02023-06-29T09:03:45ZSO:L30.55399996structure_elementcleaner02023-06-28T15:58:47ZSO:FabsmutantMESH:cleaner02023-06-28T21:08:33ZH3-530.6801788structure_elementcleaner02023-06-29T09:04:01ZSO:variable domains0.5376354evidencecleaner02023-06-29T07:48:27ZDUMMY:TmsDISCUSStitle_143340DiscussionDISCUSSparagraph43351This is the first report of a systematic structural investigation of a phage germline library. The 16 Fab structures offer a unique look at all pairings of 4 different HCs (H1-69, H3-23, H3-53, and H5-51) and 4 different LCs (L1-39, L3-11, L3-20 and L4-1), all with the same CDR H3. The structural data set taken as a whole provides insight into how the backbone conformations of the CDRs of a specific heavy or light chain vary when it is paired with 4 different light or heavy chains, respectively. A large variability in the CDR conformations for the sets of HCs and LCs is observed. In some cases the CDR conformations for all members of a set are virtually identical, for others subtle changes occur in a few members of a set, and in some cases larger deviations are observed within a set. The five variants that crystallized with 2 copies of the Fab in the asymmetric unit serve somewhat as controls for the influence of crystal packing on the conformations of the CDRs. In four of the 5 structures the CDR conformations are consistent. In only one case, that of H1-69:L3-20 (the lowest resolution structure), do we see differences in the conformations of the 2 copies of CDRs H1 and L1. This variability is likely a result of 2 factors, crystal packing interactions and internal instability of the variable domain.experimental_methodMESH:cleaner02023-06-29T09:14:19Zsystematic structural investigation0.9177982experimental_methodcleaner02023-06-29T09:09:11ZMESH:phage germline library0.16469127structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.9961118evidencecleaner02023-06-29T08:43:56ZDUMMY:structures0.99849904structure_elementcleaner02023-06-28T16:11:33ZSO:HCs0.8344044mutantcleaner02023-06-28T21:03:20ZMESH:H1-690.90730447mutantcleaner02023-06-28T21:04:18ZMESH:H3-230.9297971mutantcleaner02023-06-28T21:08:33ZMESH:H3-530.9016702mutantcleaner02023-06-28T21:05:06ZMESH:H5-510.9983071structure_elementcleaner02023-06-28T16:11:39ZSO:LCs0.64202046mutantcleaner02023-06-28T21:05:50ZMESH:L1-390.7273175mutantcleaner02023-06-28T21:06:35ZMESH:L3-110.7565879mutantcleaner02023-06-28T21:07:16ZMESH:L3-20mutantMESH:cleaner02023-06-28T21:08:01ZL4-10.99818146structure_elementcleaner02023-06-28T16:25:23ZSO:CDR0.96377325structure_elementcleaner02023-06-28T19:05:17ZSO:H30.9447263evidencecleaner02023-06-29T08:44:01ZDUMMY:structural data0.9982551structure_elementcleaner02023-06-28T19:04:38ZSO:CDRsstructure_elementSO:cleaner02023-06-28T16:13:49Zlight chainstructure_elementSO:cleaner02023-06-28T15:56:24Zheavy chains0.9916883structure_elementcleaner02023-06-28T16:25:23ZSO:CDR0.9959287structure_elementcleaner02023-06-28T16:11:33ZSO:HCs0.9971118structure_elementcleaner02023-06-28T16:11:39ZSO:LCs0.9891389structure_elementcleaner02023-06-28T16:25:23ZSO:CDR0.98784024experimental_methodcleaner02023-06-29T09:09:15ZMESH:crystallized0.1982733structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.9961863structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.99503726evidencecleaner02023-06-29T08:44:06ZDUMMY:structures0.96922016structure_elementcleaner02023-06-28T16:25:23ZSO:CDRcomplex_assemblyGO:cleaner02023-06-28T21:20:21ZH1-69:L3-200.9952447evidencecleaner02023-06-29T08:44:10ZDUMMY:structure0.99505025structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.9711269structure_elementcleaner02023-06-28T19:04:12ZSO:H10.9512453structure_elementcleaner02023-06-28T16:26:32ZSO:L10.9851179structure_elementcleaner02023-06-29T09:03:48ZSO:variable domainDISCUSSparagraph44673For the CDRs with canonical structures, the largest changes in conformation occur for CDR H1 of H1-69 and H3-53. The other 2 HCs, H3-23 and H5-51, have canonical structures that are remarkably well conserved (Fig. 1). Of the 4 HCs, H1-69 has the greatest number of canonical structure assignments (Table 2). H1-69 is unique in having a pair of glycine residues at positions 26 and 27, which provide more conformational freedom in CDR H1. Besides IGHV1-69, only the germlines of the VH4 family possess double glycines in CDR H1, and it will be interesting to see if they are also conformationally unstable.0.99440986structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.99743253structure_elementcleaner02023-06-28T16:25:23ZSO:CDR0.6280479structure_elementcleaner02023-06-28T19:04:12ZSO:H10.89499897mutantcleaner02023-06-28T21:03:20ZMESH:H1-690.92543626mutantcleaner02023-06-28T21:08:33ZMESH:H3-530.9889471structure_elementcleaner02023-06-28T16:11:33ZSO:HCs0.8808372mutantcleaner02023-06-28T21:04:18ZMESH:H3-230.90260917mutantcleaner02023-06-28T21:05:06ZMESH:H5-510.9224551protein_statecleaner02023-06-29T09:34:13ZDUMMY:remarkably well conserved0.95445126structure_elementcleaner02023-06-28T16:11:33ZSO:HCs0.803112mutantcleaner02023-06-28T21:03:20ZMESH:H1-690.8641219mutantcleaner02023-06-28T21:03:20ZMESH:H1-690.98897785residue_namecleaner02023-06-29T09:12:53ZSO:glycine0.9578594residue_numbercleaner02023-06-29T09:20:55ZDUMMY:260.91396666residue_numbercleaner02023-06-29T09:20:57ZDUMMY:27protein_stateDUMMY:cleaner02023-06-29T09:34:53Zconformational freedom0.99736977structure_elementcleaner02023-06-28T16:25:23ZSO:CDR0.67056143structure_elementcleaner02023-06-28T19:04:12ZSO:H10.9806967mutantcleaner02023-06-28T21:02:50ZMESH:IGHV1-690.61210406structure_elementcleaner02023-06-29T08:14:53ZSO:VH40.9653481residue_namecleaner02023-06-29T09:12:56ZSO:glycines0.99733585structure_elementcleaner02023-06-28T16:25:23ZSO:CDR0.5672671structure_elementcleaner02023-06-28T19:04:12ZSO:H10.8510997protein_statecleaner02023-06-29T09:34:57ZDUMMY:conformationally unstableDISCUSSparagraph45281Having all 16 VH:VL pairs with the same CDR H3 provides some insights into why molecular modeling efforts of CDR H3 have proven so difficult. As mentioned in the Results section, this data set is composed of 21 Fabs, since 5 of the 16 variants have 2 Fab copies in the asymmetric unit. For the 18 Fabs with complete backbone atoms for CDR H3, 10 have conformations similar to that of the parent, while the others have significantly different conformations (Fig. 6). Thus, it is likely that the CDR H3 conformation is dependent upon 2 dominating factors: 1) amino acid sequence; and 2) VH and VL context. More than half of the variants retain the conformation of the parent despite having differences in the VH:VL pairing. This subset includes 2 structures with 2 copies of the Fab in the asymmetric unit, all of which are nearly identical in conformation. This provides an internal control showing a consistency in the conformations. The remaining 8 structures exhibit “non-parental” conformations, indicating that the VH and VL context can also be a dominating factor influencing CDR H3. Importantly, there are 5 distinctive conformations in this subset. This subset also has 2 structures with 2 Fab copies in the asymmetric unit. Each pair has nearly identical conformations providing an internal check on the consistency of the conformations. Interestingly, as described earlier, these 2 pairs differ in the stem regions with the H1-69:L3-20 pair in the ‘extended’ conformation and H5-51:L4-1 pair in the ‘kinked’ conformation. The conformations are different from each other, as well as from the parent.0.901212complex_assemblycleaner02023-06-29T09:24:05ZGO:VH:VLstructure_elementSO:cleaner02023-06-28T16:25:23ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH3structure_elementSO:cleaner02023-06-28T16:25:23ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH30.19667532structure_elementcleaner02023-06-28T15:58:47ZSO:Fabs0.2617504structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.36789358structure_elementcleaner02023-06-28T15:58:47ZSO:Fabsstructure_elementSO:cleaner02023-06-28T16:25:23ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH3structure_elementSO:cleaner02023-06-28T16:25:23ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH3structure_elementSO:cleaner02023-06-29T07:50:58ZVHstructure_elementSO:cleaner02023-06-28T19:10:07ZVLcomplex_assemblyGO:cleaner02023-06-29T07:50:34ZVH:VL0.98431927evidencecleaner02023-06-29T08:44:18ZDUMMY:structures0.18366018structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.91228855evidencecleaner02023-06-29T08:44:24ZDUMMY:structuresstructure_elementSO:cleaner02023-06-29T07:50:49ZVHstructure_elementSO:cleaner02023-06-28T19:10:07ZVLstructure_elementSO:cleaner02023-06-28T16:25:23ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH30.96874285evidencecleaner02023-06-29T08:44:29ZDUMMY:structures0.16393337structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.99679464structure_elementcleaner02023-06-29T09:04:12ZSO:stem regionscomplex_assemblyGO:cleaner02023-06-28T21:20:21ZH1-69:L3-200.9944728protein_statecleaner02023-06-29T07:41:57ZDUMMY:extendedcomplex_assemblyGO:cleaner02023-06-29T07:51:40ZH5-51:L4-10.9884319protein_statecleaner02023-06-29T07:42:05ZDUMMY:kinkedDISCUSSparagraph46902The CDR H3 conformational analysis shows that, for each set of variants of one HC paired with the 4 different LCs, both “parental” and “non-parental” conformations are observed. The same variability is observed for the sets of variants composed of one LC paired with each of the 4 HCs. Thus, no patterns of conformational preference for a particular HC or LC emerge to shed any direct light on what drives the conformational differences. This finding supports the hypothesis of Weitzner et al. that the H3 conformation is controlled both by its sequence and its environment.structure_elementSO:cleaner02023-06-28T16:25:23ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH30.7433255experimental_methodcleaner02023-06-29T09:09:20ZMESH:conformational analysis0.9436985structure_elementcleaner02023-06-29T09:04:34ZSO:HC0.93943304structure_elementcleaner02023-06-28T16:11:39ZSO:LCs0.93057585structure_elementcleaner02023-06-28T16:24:55ZSO:LC0.9718633structure_elementcleaner02023-06-28T16:11:33ZSO:HCs0.8819633structure_elementcleaner02023-06-29T09:04:20ZSO:HC0.8892341structure_elementcleaner02023-06-28T16:24:55ZSO:LC0.9856926structure_elementcleaner02023-06-28T19:05:17ZSO:H3DISCUSSparagraph47486In looking at a possible correlation between the tilt angle and the conformation of CDR H3, no clear trends are observed. Two variants, H1-69:L3-20 and H3-23:L3-20, have the largest differences in the tilt angles compared to other variants as seen in Table 3. The absolute VH:VL orientation parameters for the 2 Fabs (Table S2) show significant deviation in HL, LC1 and HC2 values (2-3 standard deviations from the mean). One of the variants, H3-23:L3-20, has the CDR H3 conformation similar to the parent, but the other, H1-69:L3-20, is different.0.9763776evidencecleaner02023-06-29T08:44:51ZDUMMY:tilt anglestructure_elementSO:cleaner02023-06-28T16:25:24ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH30.9966311complex_assemblycleaner02023-06-28T21:20:21ZGO:H1-69:L3-200.9964395complex_assemblycleaner02023-06-29T07:52:58ZGO:H3-23:L3-20complex_assemblyGO:cleaner02023-06-29T07:53:33ZVH:VL0.9644147evidencecleaner02023-06-29T08:47:57ZDUMMY:orientation parametersstructure_elementSO:cleaner02023-06-28T15:58:47ZFabsevidenceDUMMY:cleaner02023-06-29T08:47:49Zdeviation0.9223454structure_elementcleaner02023-06-29T08:47:12ZSO:HL0.9130852structure_elementcleaner02023-06-29T08:47:20ZSO:LC10.92378676structure_elementcleaner02023-06-29T08:47:31ZSO:HC20.996636complex_assemblycleaner02023-06-29T07:53:07ZGO:H3-23:L3-20structure_elementSO:cleaner02023-06-28T16:25:24ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH30.99652complex_assemblycleaner02023-06-28T21:20:21ZGO:H1-69:L3-20DISCUSSparagraph48037As noted in the Results section, the 2 variants, H1-69:L3-20 and H3-23:L3-20, are outliers in terms of the tilt angle; at the same time, both have the smallest VH:VL interface. These smaller interfaces may perhaps translate to a significant deviation in how VH is oriented relative to VL than the other variants. These deviations from the other variants can also be seen to some extent in VH:VL orientation parameters in Table S2, as well as in the smaller number of residues involved in the VH:VL interfaces of these 2 variants (Fig. S5). These differences undoubtedly influence the conformation of the CDRs, in particular CDR H1 (Fig. 1A) and CDR L1 (Fig. 3C), especially with the tandem glycines and multiple serines present, respectively.complex_assemblyGO:cleaner02023-06-28T21:20:21ZH1-69:L3-20complex_assemblyGO:cleaner02023-06-29T07:53:56ZH3-23:L3-200.65877026evidencecleaner02023-06-29T08:48:01ZDUMMY:tilt angle0.9951705sitecleaner02023-06-28T20:18:37ZSO:VH:VL interface0.92253155sitecleaner02023-06-29T09:11:30ZSO:interfaces0.9880365structure_elementcleaner02023-06-29T09:04:46ZSO:VH0.9885372structure_elementcleaner02023-06-28T19:10:08ZSO:VLcomplex_assemblyGO:cleaner02023-06-29T07:54:20ZVH:VL0.99509436sitecleaner02023-06-29T09:11:34ZSO:VH:VL interfaces0.9969213structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.9970937structure_elementcleaner02023-06-28T16:25:24ZSO:CDR0.9948553structure_elementcleaner02023-06-28T19:04:12ZSO:H10.9974147structure_elementcleaner02023-06-28T16:25:24ZSO:CDR0.9947161structure_elementcleaner02023-06-28T16:26:32ZSO:L10.9838372residue_namecleaner02023-06-29T09:13:01ZSO:glycines0.986471residue_namecleaner02023-06-29T09:13:04ZSO:serinesDISCUSSparagraph48784Pairing of different germlines yields antibodies with various degrees of stability. As indicated by the melting temperatures, germlines H1-69 and H3-23 for HC and germline L1-39 for LC produce more stable Fabs compared to the other germlines in the experimental set. Structural determinants of the differential stability are not always easy to decipher. One possible explanation of the clear preference of LC germline L1-39 is that CDR L3 has smaller residues at positions 91 and 94, allowing for more room to accommodate CDR H3. Other germlines have bulky residues, Tyr, Arg and Trp, at these positions, whereas L1-39 has Ser and Thr. Various combinations of germline sequences for VL and VH impose certain constraints on CDR H3, which has to adapt to the environment. A more compact CDR L3 may be beneficial in this situation.protein_typeMESH:cleaner02023-06-28T16:03:38Zantibodies0.99466765evidencecleaner02023-06-29T08:48:10ZDUMMY:melting temperatures0.75309616mutantcleaner02023-06-28T21:03:21ZMESH:H1-690.8418204mutantcleaner02023-06-28T21:04:18ZMESH:H3-230.9949491structure_elementcleaner02023-06-29T09:05:01ZSO:HC0.4990733mutantcleaner02023-06-28T21:05:51ZMESH:L1-390.9483518structure_elementcleaner02023-06-28T16:24:55ZSO:LC0.48471507protein_statecleaner02023-06-29T09:35:47ZDUMMY:stable0.24393126structure_elementcleaner02023-06-28T15:58:47ZSO:Fabs0.9337159structure_elementcleaner02023-06-28T16:24:55ZSO:LCmutantMESH:cleaner02023-06-28T21:05:51ZL1-39structure_elementSO:cleaner02023-06-28T16:25:24ZCDRstructure_elementSO:cleaner02023-06-29T07:55:30ZL30.907399residue_numbercleaner02023-06-29T09:21:03ZDUMMY:910.9088576residue_numbercleaner02023-06-29T09:21:06ZDUMMY:94structure_elementSO:cleaner02023-06-28T16:25:24ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH30.99063313residue_namecleaner02023-06-29T09:13:07ZSO:Tyr0.9902063residue_namecleaner02023-06-29T09:13:09ZSO:Arg0.9905239residue_namecleaner02023-06-29T09:13:11ZSO:TrpmutantMESH:cleaner02023-06-28T21:05:51ZL1-390.9903556residue_namecleaner02023-06-29T09:13:14ZSO:Ser0.99004596residue_namecleaner02023-06-29T09:13:16ZSO:Thr0.8680984structure_elementcleaner02023-06-28T19:10:08ZSO:VL0.82958955structure_elementcleaner02023-06-29T09:05:03ZSO:VHstructure_elementSO:cleaner02023-06-28T16:25:24ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH30.93768626protein_statecleaner02023-06-29T09:35:55ZDUMMY:compactstructure_elementSO:cleaner02023-06-28T16:25:24ZCDRstructure_elementSO:cleaner02023-06-29T07:55:42ZL3DISCUSSparagraph49613At the other end of the stability range is LC germline L3-20, which yields antibodies with the lowest Tms. While pairings with H3-53 and H5-51 may be safely called a mismatch, those with H1-69 and H3-23 have Tms about 5-6° higher. Curiously, the 2 Fabs, H1-69:L3-20 and H3-23:L3-20, deviate markedly in their tilt angles from the rest of the panel. It is possible that by adopting extreme tilt angles the structure modulates CDR H3 and its environment, which apparently cannot be achieved solely by conformational rearrangement of the CDR. Note that most of the VH:VL interface residues are invariant; therefore, significant change of the tilt angle must come with a penalty in free energy. Yet, for the 2 antibodies, the total gain in stability merits the domain repacking.structure_elementSO:cleaner02023-06-28T16:24:55ZLC0.9399285mutantcleaner02023-06-28T21:07:17ZMESH:L3-20protein_typeMESH:cleaner02023-06-28T16:03:38Zantibodies0.826334evidencecleaner02023-06-29T08:48:15ZDUMMY:Tms0.85578394mutantcleaner02023-06-28T21:08:33ZMESH:H3-530.909995mutantcleaner02023-06-28T21:05:06ZMESH:H5-51mutantMESH:cleaner02023-06-28T21:03:21ZH1-690.83195966mutantcleaner02023-06-28T21:04:18ZMESH:H3-230.72107553evidencecleaner02023-06-29T08:48:19ZDUMMY:Tms0.25456816structure_elementcleaner02023-06-28T15:58:47ZSO:Fabscomplex_assemblyGO:cleaner02023-06-28T21:20:21ZH1-69:L3-20complex_assemblyGO:cleaner02023-06-29T07:57:01ZH3-23:L3-200.7603384evidencecleaner02023-06-28T16:02:51ZDUMMY:tilt anglesevidenceDUMMY:cleaner02023-06-28T16:02:51Ztilt angles0.9946995evidencecleaner02023-06-29T08:48:28ZDUMMY:structurestructure_elementSO:cleaner02023-06-28T16:25:24ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH30.99783033structure_elementcleaner02023-06-28T16:25:24ZSO:CDR0.9948602sitecleaner02023-06-28T20:18:37ZSO:VH:VL interfaceprotein_typeMESH:cleaner02023-06-28T16:03:38ZantibodiesDISCUSSparagraph50389Overall, the stability of the Fab, as measured by Tm, is a result of the mutual adjustment of the HC and LC variable domains and adjustment of CDR H3 to the VH:VL cleft. The final conformation represents an energetic minimum; however, in most cases it is very shallow, so that a single mutation can cause a dramatic rearrangement of the structure.0.21189184structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.99219584evidencecleaner02023-06-29T08:48:36ZDUMMY:Tm0.99844164structure_elementcleaner02023-06-29T09:05:09ZSO:HC0.99835795structure_elementcleaner02023-06-28T16:24:55ZSO:LC0.97715676structure_elementcleaner02023-06-29T09:05:17ZSO:variable domainsstructure_elementSO:cleaner02023-06-28T16:25:24ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH30.9936478sitecleaner02023-06-29T09:11:40ZSO:VH:VL cleft0.99620557evidencecleaner02023-06-29T08:48:39ZDUMMY:structureDISCUSSparagraph50737In summary, the analysis of this structural library of germline variants composed of all pairs of 4 HCs and 4LCs, all with the same CDR H3, offers some unique insights into antibody structure and how pairing and sequence may influence, or not, the canonical structures of the L1, L2, L3, H1 and H2 CDRs. Comparison of the CDR H3s reveals a large set of variants with conformations similar to the parent, while a second set has significant conformational variability, indicating that both the sequence and the structural context define the CDR H3 conformation. Quite unexpectedly, 2 of the variants, H1-69:L3-20 and H3-53:L4-1, have the ‘extended’ stem region differing from the other 14 that have a ‘kinked’ stem region. Why this is the case is unclear at present. These data reveal the difficulty of modeling CDR H3 accurately, as shown again in Antibody Modeling Assessment II. Furthermore, antibody CDRs, H3 in particular, may go through conformational changes upon binding their targets, making structural prediction for docking purposes an even more difficult task. Fortunately, for most applications of antibody modeling, such as engineering affinity and biophysical properties, an accurate CDR H3 structure is not always necessary. For those applications where accurate CDR structures are essential, such as docking, the results in this work demonstrate the importance of experimental structures. With the recent advances in expression and crystallization methods, Fab structures can be obtained rapidly.0.8765336experimental_methodcleaner02023-06-29T09:09:28ZMESH:structural library0.934844structure_elementcleaner02023-06-28T16:11:33ZSO:HCsstructure_elementSO:cleaner02023-06-29T07:57:39ZLCsstructure_elementSO:cleaner02023-06-28T16:25:24ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH3protein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.8813323evidencecleaner02023-06-29T08:48:42ZDUMMY:structure0.9966403structure_elementcleaner02023-06-28T16:26:32ZSO:L10.99470454structure_elementcleaner02023-06-28T20:09:54ZSO:L20.9959739structure_elementcleaner02023-06-29T09:05:21ZSO:L30.99525684structure_elementcleaner02023-06-28T19:04:13ZSO:H10.9918994structure_elementcleaner02023-06-28T16:26:22ZSO:H20.9839631structure_elementcleaner02023-06-28T19:04:38ZSO:CDRsstructure_elementSO:cleaner02023-06-28T16:25:24ZCDRstructure_elementSO:cleaner02023-06-29T07:58:01ZH3sstructure_elementSO:cleaner02023-06-28T16:25:24ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH3complex_assemblyGO:cleaner02023-06-28T21:20:21ZH1-69:L3-20complex_assemblyGO:cleaner02023-06-28T21:18:21ZH3-53:L4-10.87856656protein_statecleaner02023-06-29T07:41:57ZDUMMY:extended0.9895041structure_elementcleaner02023-06-29T09:05:30ZSO:stem region0.5306472protein_statecleaner02023-06-29T07:42:05ZDUMMY:kinked0.98894227structure_elementcleaner02023-06-29T09:05:32ZSO:stem regionstructure_elementSO:cleaner02023-06-28T16:25:24ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH3protein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.9935402structure_elementcleaner02023-06-28T19:04:38ZSO:CDRs0.9950309structure_elementcleaner02023-06-28T19:05:17ZSO:H3protein_typeMESH:cleaner02023-06-28T15:45:48Zantibodystructure_elementSO:cleaner02023-06-28T16:25:24ZCDRstructure_elementSO:cleaner02023-06-28T19:05:17ZH30.98669493evidencecleaner02023-06-29T08:48:51ZDUMMY:structure0.991574structure_elementcleaner02023-06-28T16:25:24ZSO:CDR0.70661396evidencecleaner02023-06-29T08:48:54ZDUMMY:structures0.9209164evidencecleaner02023-06-29T08:48:57ZDUMMY:structures0.95461094experimental_methodcleaner02023-06-29T09:09:54ZMESH:expression and crystallization methods0.17987959structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.9961416evidencecleaner02023-06-29T08:49:00ZDUMMY:structuresDISCUSSparagraph52257The set of 16 germline Fab structures offers a unique dataset to facilitate software development for antibody modeling. The results essentially support the underlying idea of canonical structures, indicating that most CDRs with germline sequences tend to adopt predefined conformations. From this point of view, a novel approach to design combinatorial antibody libraries would be to cover the range of CDR conformations that may not necessarily coincide with the germline usage in the human repertoire. This would insure more structural diversity, leading to a more diverse panel of antibodies that would bind to a broad spectrum of targets.0.23744409structure_elementcleaner02023-06-28T15:59:05ZSO:Fab0.99531835evidencecleaner02023-06-29T08:49:04ZDUMMY:structuresprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.9604034evidencecleaner02023-06-29T08:49:07ZDUMMY:structures0.99709547structure_elementcleaner02023-06-28T19:04:38ZSO:CDRsprotein_typeMESH:cleaner02023-06-28T15:45:48Zantibody0.993736structure_elementcleaner02023-06-28T16:25:24ZSO:CDR0.99389875speciescleaner02023-06-28T16:04:00ZMESH:humanprotein_typeMESH:cleaner02023-06-28T16:03:38ZantibodiesMETHODStitle_152900Materials and methodsMETHODStitle_252922Fab production, purification and crystallizationMETHODSparagraph52971The production, purification and crystallization of the Fabs reported in this article were described previously. Briefly, the 16 Fabs were produced by combining 4 different HC and 4 different LC germline constructs. The human HC germlines were IGHV1-69 (H1-69), IGHV3-23 (H3-23), IGHV3-53 (H3-53) and IGHV5-51 (H5-51) in the IMGT nomenclature. The human LC germlines were IGKV1-39 (L1-39), IGKV3-11 (L3-11), IGKV3-20 (L3-20) and IGKV4-1 (L4-1) corresponding to O12, L6, A27 and B3 in the V-BASE nomenclature. CDR H3 of the anti-CCL2 antibody CNTO 888 with the amino acid sequence ARYDGIYGELDF was used in all Fab constructs. The J region genes were IGHJ1 for the HC and IGKJ1 for the LC for all Fabs. Human IgG1 and κ constant regions were used in all Fab constructs. A 6xHis tag was added to the C-terminus of the HC to facilitate purification.METHODSparagraph53822The Fabs were expressed in HEK 293E cells and purified by affinity and size-exclusion chromatography. For crystallization, the Fabs were dialyzed into 20 mM Tris buffer, pH 7.4, with 50 mM NaCl and concentrated to 12-18 mg/mL. Automated crystallization screening was carried out using the vapor diffusion method at 20°C with an Oryx4 (Douglas Instruments) or a Mosquito (TTP Labtech) crystallization robot in a sitting drop format using Corning 3550 plates. Initial screening was carried out with an in-house 192-well screen optimized for Fab crystallization and the Hampton 96-well Crystal Screen HT (Hampton Research). For the majority of the Fabs, the crystallization protocol employed microseed matrix screening using self-seeding or cross-seeding approaches. A summary of the final crystallization conditions for each of the Fabs is presented in Table 1.METHODStitle_254687X-ray data collectionMETHODSparagraph54709For 13 of the Fab crystals, X-ray data collection was carried out at Janssen Research and Development, LLC using a Rigaku MicroMax™-007HF microfocus X-ray generator equipped with a Saturn 944 CCD detector and an X-stream™ 2000 cryocooling system (Rigaku), and for the remaining 3, X-ray data collection was carried out at the Advanced Photon Source (APS) synchrotron at Argonne National Laboratory using the IMCA 17-ID beamline with a Pilatus 6M detector. For X-ray data collection, the Fab crystals were soaked for a few seconds in a cryo-protectant solution containing the corresponding mother liquor supplemented with 17-25% glycerol (Table S1). The crystals for which data were collected in-house were flash cooled in the stream of nitrogen at 100 K. Crystals sent to the APS were flash cooled in liquid nitrogen prior to shipping them to the synchrotron. Diffraction data for all variants were processed with the program XDS. X-ray data statistics are given in Table 1.METHODStitle_255691Structure determinationMETHODSparagraph55715A summary of the methods used in the structure solution and refinement of the 16 Fabs is presented in Table S1. Twelve of the structures were solved by molecular replacement with Phaser using different combinations of search models for the VH, VL and constant domains. Four of the structures, H3-53:L1-39, H3-53:L3-11, H5-51:L1-39 and H5-51:L3-11, were solved by direct replacement followed by rigid body refinement with REFMAC. All structures were refined using REFMAC. Model adjustments were carried out using the program Coot. The refinement statistics are given in Table 1. Other crystallographic calculations were performed with the CCP4 suite of programs. The structural figures were prepared using the PyMOL Molecular Graphics System, Version 1.0 (Schrödinger, LLC).METHODStitle_256492Structural analysisMETHODSparagraph56512The canonical structure assignments (Table 2) were made using PyIgClassify, an online canonical structure classification tool (http://dunbrack2.fccc.edu/pyigclassify/) that uses the rules set forth by Dunbrack and coworkers.METHODSparagraph56738The conformational variability within the CDRs was assessed by calculating the root-mean-square deviation (rmsd) from the average structure that was generated after superposition of all structures of the set using the main-chain atoms of the CDR in question. The rmsd was calculated for all main-chain atoms (N, CA, C, O) of the CDR.METHODSparagraph57072The contact surface areas of the VH and VL domains at the VH:VL inteface were computed with the CCP4 program PISA. The surface complementarity of the VH and VL domains was computed using the CCP4 program SC.METHODStitle_257280VH:VL tilt anglesMETHODSparagraph57298The orientation of the VH domain with respect to the VL domain was assessed using 2 different approaches. The first approach calculates the 6 VH:VL orientation parameters that describe the VH:VL relationship according to Dunbar and co-workers using a script downloaded from the website (http://opig.stats.ox.ac.uk/webapps/abangle). The six parameters include 5 angles, HL, H1, H2, L1 and L2, and a distance, dc. These parameters are derived by first defining 2 planes, one for each domain, based on core residues in the domains. The distance between the planes, dc, is determined along a vector between the planes that is used to establish a consistent coordinate system. The torsion angle between the domains, HL, is much like the VH:VL packing angle defined by Abhinandan and Martin. The tilt of one domain relative to the other is defined by the HC1 and LC1 angles, and the twist of one domain relative to the other is defined by the HC2 and LC2 angles.METHODSparagraph58255The second approach calculates the difference in the tilt angle between pairs of Fvs, which reflects the relative orientation between the VH and VL domains. The difference with respect to the reference structure is calculated by sequential root-mean-square superposition of the VL and VH domains using β-sheet core Cα positions (Chothia numbering scheme): 3–13, 18–25, 33–38, 43–49, 61–67, 70–76, 85–90, 97–103 for VL; 3–7, 18–24, 34–40, 44–51, 56–59, 67–72, 77–82a, 87–94, 102–110 for VH. The κ angle in the spherical polar angular system (ω, ϕ, κ) of the latter transformation is the difference in the tilt angle.METHODStitle_258911Differential scanning calorimetryMETHODSparagraph58945DSC experiments were performed on a VP-capillary DSC system (MicroCal Inc., Northampton, MA) in which temperature differences between the reference and sample cell are continuously measured and calibrated to power units. Samples were heated from 10°C to 95°C at a heating rate of 60°C/hour. The pre-scan time was 15 minutes and the filtering period was 10 seconds. The concentration used in the DSC experiments was about 0.4 mg/mL in phosphate-buffered saline. Analysis of the resulting thermograms was performed using MicroCal Origin 7 software. Melting temperature of proteins was determined by deconvolution of the DSC scans using non-2 state model in the MicroCal Origin 7 software. Scans were deconvoluted using a non-2 state model with either 1-step transition or 2-step transition depending on the number of resolved peaks observed in a scan.METHODStitle_259799Accession numbersMETHODSparagraph59817Atomic coordinates and structure factors have been deposited in the Protein Data Bank with accession numbers 4KMT, 5I15, 5I16, 5I17, 5I18, 5I19, 5I1A, 5I1C, 5I1D, 5I1E, 5I1G, 5I1H, 5I1I, 5I1J, 5I1K and 5I1L.SUPPLtitle_160025Supplementary MaterialCOMP_INTtitle_160048Disclosure of potential conflicts of interestCOMP_INTparagraph60094No potential conflicts of interest were disclosed.REFtitle60145References197204surname:Reichert;given-names:JM10.1080/19420862.2015.112558326651519REFMAbsref8201660156Antibodies to watch in 2016211250surname:Wu;given-names:TTsurname:Kabat;given-names:EA10.1084/jem.132.2.2115508247REFJ Exp Medref132197060184An analysis of the sequences of the variable regions of Bence Jones proteins and myeloma light chains and their implications for antibody complementarity901917surname:Chothia;given-names:Csurname:Lesk;given-names:AM10.1016/0022-2836(87)90412-83681981REFJ Mol Biolref196198760338Canonical structures for the hypervariable regions of immunoglobulins877883surname:Chothia;given-names:Csurname:Lesk;given-names:AMsurname:Tramontano;given-names:Asurname:Levitt;given-names:Msurname:Smith-Gill;given-names:SJsurname:Air;given-names:Gsurname:Sheriff;given-names:Ssurname:Padlan;given-names:EAsurname:Davies;given-names:Dsurname:Tulip;given-names:WR10.1038/342877a02687698REFNatureref342198960408Conformations of immunoglobulin hypervariable regions799817surname:Chothia;given-names:Csurname:Lesk;given-names:AMsurname:Gherardi;given-names:Esurname:Tomlinson;given-names:IMsurname:Walter;given-names:Gsurname:Marks;given-names:JDsurname:Llewelyn;given-names:MBsurname:Winter;given-names:G10.1016/0022-2836(92)90224-81404389REFJ Mol Biolref227199260462Structural repertoire of the human VH segments46284638surname:Tomlinson;given-names:IMsurname:Cox;given-names:JPsurname:Herardi;given-names:GEsurname:Lesk;given-names:AMsurname:Chothia;given-names:C7556106REFEMBO Jref14199560509The structural repertoire of the human V kappa domain497504surname:Vargas-Madrazo;given-names:Esurname:Lara-Ochoa;given-names:Fsurname:Almagro;given-names:JC10.1006/jmbi.1995.06337490765REFJ Mol Biolref254199560563Canonical structure repertoire of the antigen-binding site of immunoglobulins suggests strong geometrical restrictions associated to the mechanism of immune recognition927948surname:Al-Lazikani;given-names:Bsurname:Lesk;given-names:AMsurname:Chothia;given-names:C10.1006/jmbi.1997.13549367782REFJ Mol Biolref273199760732Standard conformations for the canonical structures of immunoglobulins337354surname:Collis;given-names:AVsurname:Brouwer;given-names:APsurname:Martin;given-names:AC10.1016/S0022-2836(02)01222-612488099REFJ Mol Biolref325200360803Analysis of the antigen combining site: correlations between length and sequence composition of the hypervariable loops and the nature of the antigen235242surname:Berman;given-names:HMsurname:Westbrook;given-names:Jsurname:Feng;given-names:Zsurname:Gilliland;given-names:Gsurname:Bhat;given-names:TNsurname:Weissig;given-names:Hsurname:Shindyalov;given-names:INsurname:Bourne;given-names:PE10.1093/nar/28.1.23510592235REFNucleic Acids Resref28200060953The Protein Data Bank800815surname:Martin;given-names:ACsurname:Thornton;given-names:JM10.1006/jmbi.1996.06178947577REFJ Mol Biolref263199660975Structural families in loops of homologous proteins: automatic classification, modelling and application to antibodies228256surname:North;given-names:Bsurname:Lehmann;given-names:Asurname:Dunbrack;given-names:RL10.1016/j.jmb.2010.10.03021035459REFJ Mol Biolref406201161094A new clustering of antibody CDR loop conformationsD432D438surname:Adolf-Bryfogle;given-names:Jsurname:Xu;given-names:Qsurname:North;given-names:Bsurname:Lehmann;given-names:Asurname:Dunbrack;given-names:RL10.1093/nar/gku110625392411REFNucleic Acids Resref43201561146PyIgClassify: a database of antibody CDR structural classifications18surname:Shirai;given-names:Hsurname:Kidera;given-names:Asurname:Nakamura;given-names:H10.1016/S0014-5793(96)01252-58980108REFFEBS Lettref399199661214Structural classification of CDR-H3 in antibodies916surname:Morea;given-names:Vsurname:Tramontano;given-names:Asurname:Rustici;given-names:Msurname:Chothia;given-names:Csurname:Lesk;given-names:AM10.1016/S0301-4622(96)02266-19468606REFBiophys Chemref68199761264Antibody structure, prediction and redesign269294surname:Morea;given-names:Vsurname:Tramontano;given-names:Asurname:Rustici;given-names:Msurname:Chothia;given-names:Csurname:Lesk;given-names:AM10.1006/jmbi.1997.14429466909REFJ Mol Biolref275199861308Conformations of the third hypervariable region in the VH domain of immunoglobulins608620surname:Kuroda;given-names:Dsurname:Shirai;given-names:Hsurname:Kobori;given-names:Msurname:Nakamura;given-names:H10.1002/prot.2208718473362REFProteinsref73200861392Structural classification of CDR-H3 revisited: a lesson in antibody modeling302311surname:Weitzner;given-names:BDsurname:Dunbrack;given-names:RLsurname:Gray;given-names:JJ10.1016/j.str.2014.11.01025579815REFStructureref23201561469The origin of CDR H3 structural diversity30503066surname:Almagro;given-names:JCsurname:Beavers;given-names:MPsurname:Hernandez-Guzman;given-names:Fsurname:Maier;given-names:Jsurname:Shaulsky;given-names:Jsurname:Butenhof;given-names:Ksurname:Labute;given-names:Psurname:Thorsteinson;given-names:Nsurname:Kelly;given-names:Ksurname:Teplyakov;given-names:A10.1002/prot.2313021935986REFProteinsref79201161511Antibody modeling assessment15531562surname:Almagro;given-names:JCsurname:Teplyakov;given-names:Asurname:Luo;given-names:Jsurname:Sweet;given-names:Rsurname:Kodangattil;given-names:Ssurname:Hernadez-Guzman;given-names:Fsurname:Gilliland;given-names:GL10.1002/prot.2456724668560REFProteinsref82201461540Second Antibody Modeling Assessment (AMA-II)15631582surname:Teplyakov;given-names:Asurname:Luo;given-names:Jsurname:Obmolova;given-names:Gsurname:Malia;given-names:TJsurname:Sweet;given-names:Rsurname:Stanfield;given-names:RLsurname:Kodangattil;given-names:Ssurname:Almagro;given-names:JCsurname:Gilliland;given-names:GL10.1002/prot.2455424633955REFProteinsref82201461585Antibody modeling assessment II. Structures and models385396surname:Shi;given-names:Lsurname:Wheeler;given-names:JCsurname:Sweet;given-names:RWsurname:Lu;given-names:Jsurname:Luo;given-names:Jsurname:Tornetta;given-names:Msurname:Whitaker;given-names:Bsurname:Reddy;given-names:Rsurname:Brittingham;given-names:Rsurname:Borozdina;given-names:L10.1016/j.jmb.2010.01.03420114051REFJ Mol Biolref397201061640De novo selection of high-affinity antibodies from synthetic fab libraries displayed on phage as pIX fusion proteins895901surname:de Wildt;given-names:RMsurname:Hoet;given-names:RMsurname:van Venrooij;given-names:WJsurname:Tomlinson;given-names:IMsurname:Winter;given-names:G10.1006/jmbi.1998.23969887257REFJ Mol Biolref285199961757Analysis of heavy and light chain pairings indicates that receptor editing shapes the human antibody repertoire227233surname:Obmolova;given-names:Gsurname:Teplyakov;given-names:Asurname:Malia;given-names:TJsurname:Grygiel;given-names:TLsurname:Sweet;given-names:Rsurname:Snyder;given-names:LAsurname:Gilliland;given-names:GL10.1016/j.molimm.2012.03.02222487721REFMol Immunolref51201261869Structural basis for high selectivity of anti-CCL2 neutralizing antibody CNTO 88811071115surname:Obmolova;given-names:Gsurname:Malia;given-names:TJsurname:Teplyakov;given-names:Asurname:Sweet;given-names:Rsurname:Gilliland;given-names:GL10.1107/S2053230X1401255225084393REFActa CrystallogrrefF70201461951Protein crystallization with microseed matrix screening: application to human germline antibody Fabs175182surname:Tramontano;given-names:Asurname:Chothia;given-names:Csurname:Lesk;given-names:AM10.1016/S0022-2836(05)80102-02118959REFJ Mol Biolref215199062052Framework residue-71 is a major determinant of the position and conformation of the 2nd hypervariable region in the VH domains of immunoglobulins483498surname:Mas;given-names:MTsurname:Smith;given-names:KCsurname:Yarmush;given-names:DLsurname:Aisaka;given-names:Ksurname:Fine;given-names:RM10.1002/prot.3401404091438186REFProteinsref14199262198Modeling the anti-CEA antibody combining site by homology and conformational search8393surname:Stanfield;given-names:RLsurname:Takimoto-Kamimura;given-names:Msurname:Rini;given-names:JMsurname:Profy;given-names:ATsurname:Wilson;given-names:IA10.1016/0969-2126(93)90024-B8069628REFStructureref1199362282Major antigen-induced domain rearrangements in an antibody941953surname:Narayanan;given-names:Asurname:Sellers;given-names:BDsurname:Jacobson;given-names:MP10.1016/j.jmb.2009.03.04319324053REFJ Mol Biolref388200962341Energy-based analysis and prediction of the orientation between light- and heavy-chain antibody variable domains689697surname:Abhinandan;given-names:KRsurname:Martin;given-names:AC10.1093/protein/gzq04320591902REFProtein Eng Des Selref23201062454Analysis and prediction of VH/VL packing in antibodies611620surname:Dunbar;given-names:Jsurname:Fuchs;given-names:Asurname:Shi;given-names:Jsurname:Deane;given-names:CM10.1093/protein/gzt02023708320REFProtein Eng Des Selref26201362509ABangle: characterising the VH-VL orientation in antibodies774797surname:Krissinel;given-names:Esurname:Henrick;given-names:K10.1016/j.jmb.2007.05.02217681537REFJ Mol Biolref372200762569Inference of macromolecular assemblies from crystalline state946950surname:Lawrence;given-names:MCsurname:Colman;given-names:PM10.1006/jmbi.1993.16488263940REFJ Mol Biolref234199362631Shape complementarity at protein/protein interfaces959965surname:Rini;given-names:JMsurname:Schulze-Gahmen;given-names:Usurname:Wilson;given-names:IA10.1126/science.15462931546293REFScienceref255199262683Structural evidence for induced fit as a mechanism for antibody-antigen recognitionAppendix 1Psurname:Lefranc;given-names:MP10.1002/0471142735.ima01ps4018432650REFCurr Protoc Immunolref200162767Nomenclature of the human immunoglobulin genessurname:Tomlinson;given-names:IMsurname:Williams;given-names:SCsurname:Ignatovitch;given-names:Osurname:Corbett;given-names:SJsurname:Winter;given-names:GREFV BASE Sequence Directory, MRC Centre for Protein Engineeringref199862814182189surname:Zhao;given-names:Ysurname:Gutshall;given-names:Lsurname:Jiang;given-names:Hsurname:Baker;given-names:Asurname:Beil;given-names:Esurname:Obmolova;given-names:Gsurname:Carton;given-names:Jsurname:Taudte;given-names:Ssurname:Amegadzie;given-names:B10.1016/j.pep.2009.04.01219442740REFProtein Expr Purifref67200962815Two routes for production and purification of Fab fragments in biopharmaceutical discovery research: papain digestion of mAb and transient expression in mammalian cells550554surname:D'Arcy;given-names:Asurname:Villard;given-names:Fsurname:Marsh;given-names:M10.1107/S090744490700765217372361REFActa CrystallogrrefD63200762984An automated microseed matrix-screening method for protein crystallization927933surname:Obmolova;given-names:Gsurname:Malia;given-names:TJsurname:Teplyakov;given-names:Asurname:Sweet;given-names:Rsurname:Gilliland;given-names:GL10.1107/S090744491002604120693692REFActa CrystallogrrefD66201063059Promoting crystallization of antibody-antigen complexes via microseed matrix screening125132surname:Kabsch;given-names:W10.1107/S090744490904733720124692REFActa CrystallogrrefD66201063146XDS658674surname:McCoy;given-names:AJsurname:Grosse-Kunstleve;given-names:RWsurname:Adams;given-names:PDsurname:Winn;given-names:MDsurname:Storoni;given-names:LCsurname:Read;given-names:RJ10.1107/S002188980702120619461840REFJ Appl Crystallogrref40200763150Phaser crystallographic software355367surname:Murshudov;given-names:GNsurname:Skubak;given-names:Psurname:Lebedev;given-names:AAsurname:Pannu;given-names:NSsurname:Steiner;given-names:RAsurname:Nicholls;given-names:RAsurname:Winn;given-names:MDsurname:Long;given-names:Fsurname:Vagin;given-names:AA10.1107/S090744491100131421460454REFActa CrystallogrrefD67201163183REFMAC5 for the refinement of macromolecular crystal structures486501surname:Emsley;given-names:Psurname:Lohkamp;given-names:Bsurname:Scott;given-names:WGsurname:Cowtan;given-names:K10.1107/S090744491000749320383002REFActa CrystallogrrefD66201063247Features and development of Coot235242surname:Winn;given-names:MDsurname:Ballard;given-names:CCsurname:Cowtan;given-names:KDsurname:Dodson;given-names:EJsurname:Emsley;given-names:Psurname:Evans;given-names:PRsurname:Keegan;given-names:RMsurname:Krissinel;given-names:EBsurname:Leslie;given-names:AGsurname:McCoy;given-names:A10.1107/S090744491004574921460441REFActa CrystallogrrefD67201163280Overview of the CCP4 suite and current developments