# Gene transcriptions/CAREs

"Some other cis-acting elements, such as pyrimidine boxes (GGTTTT) and TAT boxes (TATCCAT), are usually present in the vicinity of the GARE sequence of genes regulated by GA in cereal aleurone cells (Gubler and Jacobsen 1992; Cercos et al. 1999; Tsuji et al. 2006). For example, GARE and a novel CARE (CAACTC regulatory elements) elements are present in the promoter of rice RAmy1A (Ueguchi-Tanaka et al. 2000; Sutoh and Yamauchi 2003). Cis-element analyses have shown that the OsGAMYB protein activates RAmy1A expression through interaction with GARE in the promoter (Washio 2003). In addition, GARE and CARE are also present in a cysteine proteinase gene REP-1, which is expressed in rice aleurone and is induced by GAs and repressed by ABA. These two elements have been identified as necessary and sufficient for conferring GA inducibility of the REP-1 promoter. Mutations of CARE in the promoters of RAmy1A and REP-1 result in loss of GA inducibility and GAMYB transactivation, suggesting that CARE is a regulatory element for the GA-inducible expression of hydrolase genes in germinating seeds (Sutoh and Yamauchi 2003)."

## CARE sampling of A1BG promoters

For the Basic programs (starting with SuccessablesCARE.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), including extending the number of nts from 958 to 4445, the programs are, are looking for, and found:
* negative strand in the negative direction (from ZSCAN22 to A1BG) is SuccessablesCARE--.bas, looking for 3'-CAACTC-5', 1, 3'-CAACTC-5', 86,
* negative strand in the positive direction (from ZNF497 to A1BG) is SuccessablesCARE-+.bas, looking for 3'-CAACTC-5', 1, 3'-CAACTC-5', 3292,
* positive strand in the negative direction is SuccessablesCARE+-.bas, looking for 3'-CAACTC-5', 0,
* positive strand in the positive direction is SuccessablesCARE++.bas, looking for 3'-CAACTC-5', 0,
* complement, negative strand, negative direction is SuccessablesCAREc--.bas, looking for 3'-GTTGAG-5', 0,
* complement, negative strand, positive direction is SuccessablesCAREc-+.bas, looking for 3'-GTTGAG-5', 0,
* complement, positive strand, negative direction is SuccessablesCAREc+-.bas, looking for 3'-GTTGAG-5', 1, 3'-GTTGAG-5', 86,
* complement, positive strand, positive direction is SuccessablesCAREc++.bas, looking for 3'-GTTGAG-5', 1, 3'-GTTGAG-5', 3292,
* inverse complement, negative strand, negative direction is SuccessablesCAREci--.bas, looking for 3'-GAGTTG-5', 1, 3'-GAGTTG-5', 1406,
* inverse complement, negative strand, positive direction is SuccessablesCAREci-+.bas, looking for 3'-GAGTTG-5', 0,
* inverse complement, positive strand, negative direction is SuccessablesCAREci+-.bas, looking for 3'-GAGTTG-5', 4, 3'-GAGTTG-5', 2592, 3'-GAGTTG-5', 2704, 3'-GAGTTG-5', 3115, 3'-GAGTTG-5', 4096,
* inverse complement, positive strand, positive direction is SuccessablesCAREci++.bas, looking for 3'-GAGTTG-5', 2, 3'-GAGTTG-5', 1621, 3'-GAGTTG-5', 3290,
* inverse, negative strand, negative direction, is SuccessablesCAREi--.bas, looking for 3'-CTCAAC-5', 4, 3'-CTCAAC-5', 2592, 3'-CTCAAC-5', 2704, 3'-CTCAAC-5', 3115, 3'-CTCAAC-5', 4096,
* inverse, negative strand, positive direction, is SuccessablesCAREi-+.bas, looking for 3'-CTCAAC-5', 2, 3'-CTCAAC-5', 1621, 3'-CTCAAC-5', 3290,
* inverse, positive strand, negative direction, is SuccessablesCAREi+-.bas, looking for 3'-CTCAAC-5', 1, 3'-CTCAAC-5', 1406,
* inverse, positive strand, positive direction, is SuccessablesCAREi++.bas, looking for 3'-CTCAAC-5', 0.