# Gene transcriptions/Boxes/Prolamins

"The BPBF [barley prolamin-box (P-box) binding factor] expressed in bacteria as a GST-fusion binds a P-box 5′-TGTAAAG-3′ containing oligonucleotide derived from the promoter region of anHor2gene."
"The primary structure of hordein [barley prolamins] polypeptides is closely related to that of prolamins from other grass species from the Pooideae subfamily, such as wheat and rye (Shewry & Tatham 1990;Shewry et al. 1995)."

## Consensus sequences

"The close evolutionary relationship is also manifested by the conservation of a putative regulatory element in their gene promoters, the endosperm box (Forde et al. 1985;Kreis et al. 1985). This conserved region consists of two motifs, a 7 bp element (5′TGTAAAG3′) termed the Prolamin Box (P-box) or endosperm motif (EM) followed at a distance of up to 8 nucleotides by the GCN4-like motif (GLM) which has the 5′(G/A)TGA(G/C)TCA(T/C)3′ consensus sequence (reviewed by Müller et al. 1995)."

## A1BG sampling

For the Basic programs (starting with SuccessablesProl.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
* negative strand in the negative direction (from ZSCAN22 to A1BG) is SuccessablesProl1--.bas, looking for 3'-TG(A/T)AAAG-5', 1, 3'-TGTAAAG-5', 2884,
* negative strand in the positive direction (from ZNF497 to A1BG) is SuccessablesProl1-+.bas, looking for 3'-TG(A/T)AAAG-5', 1, 3'-TGAAAAG-5', 489,
* positive strand in the negative direction is SuccessablesProl1+-.bas, looking for 3'-TG(A/T)AAAG-5', 1, 3'-TGAAAAG-5', 1627,
* positive strand in the positive direction is SuccessablesProl1++.bas, looking for 3'-TG(A/T)AAAG-5', 0,
* complement, negative strand, negative direction is SuccessablesProl1c--.bas, looking for 3'-AC(A/T)TTTC-5', 1, 3'-ACTTTTC-5', 1627,
* complement, negative strand, positive direction is SuccessablesProl1c-+.bas, looking for 3'-AC(A/T)TTTC-5', 0,
* complement, positive strand, negative direction is SuccessablesProl1c+-.bas, looking for 3'-AC(A/T)TTTC-5', 1, 3'-ACATTTC-5', 2884,
* complement, positive strand, positive direction is SuccessablesProl1c++.bas, looking for 3'-AC(A/T)TTTC-5', 1, 3'-ACTTTTC-5', 489,
* inverse complement, negative strand, negative direction is SuccessablesProl1ci--.bas, looking for 3'-CTTT(A/T)CA-5', 0,
* inverse complement, negative strand, positive direction is SuccessablesProl1ci-+.bas, looking for 3'-CTTT(A/T)CA-5', 0,
* inverse complement, positive strand, negative direction is SuccessablesProl1ci+-.bas, looking for 3'-CTTT(A/T)CA-5', 0,
* inverse complement, positive strand, positive direction is SuccessablesProl1ci++.bas, looking for 3'-CTTT(A/T)CA-5', 0,
* inverse, negative strand, negative direction, is SuccessablesProl1i--.bas, looking for 3'-GAAA(A/T)GT-5', 0,
* inverse, negative strand, positive direction, is SuccessablesProl1i-+.bas, looking for 3'-GAAA(A/T)GT-5', 0,
* inverse, positive strand, negative direction, is SuccessablesProl1i+-.bas, looking for 3'-GAAA(A/T)GT-5', 0,
* inverse, positive strand, positive direction, is SuccessablesProl1i++.bas, looking for 3'-GAAA(A/T)GT-5', 0.