# Genetic engineering

Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms.

## Quotes

* By using genetic engineering, biological researchers have already developed new weapons that are much more effective than their natural counterparts. Countless examples from the daily work of molecular biologists could be presented here, not least the introduction of antibiotic resistance into bacterial pathogens, which today is routine work in almost any microbiology laboratory. Indeed, many research projects in basic science show—sometimes unwillingly and unwittingly—how to overcome current scientific and technological limits in the military use of pathogenic agents. Furthermore, genetic engineering is not merely a theoretical possibility for future biowarfare: it has already been applied in past weapons programmes, particularly in the former Soviet Union. One example is the USSR's 'invisible anthrax', resulting from the introduction of an alien gene into Bacillus anthracis that altered its immunological properties (Pomerantsev et al., 1997). Existing vaccines proved to be ineffective against this new genetically engineered strain.
** Jan van Aken and Edward Hammond, “Genetic engineering and biological weapons“, EMBO Rep. 2003 Jun; 4(Suppl 1): S57–S60.
* Molecular biology and genetic engineering are still in their infancy, and more technical possibilities will arise in the years to come—for military abuse too (Fraser & Dando, 2001). More efficient classical biowarfare agents will probably have only a marginal role, even if the genetically engineered 'superbug' is still routinely featured in newspaper reports. More likely and more alarming are weapons for new types of conflicts and warfare scenarios, namely low-intensity warfare or secret operations, for economic warfare or for sabotage activities. To prevent the hostile exploitation of biology now and forever, a bundle of measures must be taken, from strengthening the Biological and Toxin Weapons Convention to building an awareness in the scientific community about the possibilities and dangers of abuse. Any kind of biotechnological or biomedical research, development or production must be performed in an internationally transparent and controlled manner. In cases in which military abuse seems to be imminent and likely, alternative ways to pursue the same research goal have to be developed. Furthermore, as we mentioned above with regard to the smallpox genome sequence, it might also be necessary to apply restrictions to certain research and/or publications.
** Jan van Aken and Edward Hammond, “Genetic engineering and biological weapons“, EMBO Rep. 2003 Jun; 4(Suppl 1): S57–S60.
* Can genetic engineers restore a rapid worldwide rise in grainland productivity? This prospect is not promising simply because plant breeders using traditional techniques have largely exploited the genetic potential for increasing the share of photosynthate that goes into seed. Once this is pushed close to its limit, the remaining options tend to be relatively small, clustering around efforts to raise the plant’s tolerance of various stresses, such as drought or soil salinity. One major option left to scientists is to increase the efficiency of the process of photosynthesis itself—something that has thus far remained beyond their reach.
** Lester R. Brown, Outgrowing the Earth (2005), Ch. 4 : Raising the Earth’s Productivity
* You can stop splitting the atom; you can stop visiting the moon; you can stop using aerosols; you may even decide not to kill entire populations by the use of a few bombs. But you cannot recall a new form of life.
** Erwin Chargaff, Letter to the editor of Science (1976). Quoted in Rose M. Morgan, The Genetics Revolution (2006), 3.
* Transferring genes between utterly unrelated species takes us into the realms that belong to God, and to God alone.
** Charles, Prince of Wales, cited in Awake! magazine 2000, 4/22. − −
* When scientists began creating adenoviral vectors in the 1980s, most worked with a particular kind of adenovirus called Ad5, which ubiquitously infects humans and causes the common cold. Researchers stripped Ad5 of the genes it needed to replicate and inserted those genes into genetically engineered cell lines. That ensured that the modified viruses could be grown only in these special cells in the lab. It also opened up space in the Ad5 genome for scientists to stitch in new genes of their choosing. Many scientists hoped to use Ad5 to deliver a human gene that could correct rare genetic mutations—an approach called gene therapy. Those efforts came to a grinding halt in 1999 when a teenage boy with a rare genetic liver disease died after receiving an injection of an Ad5-based gene therapy, which had been designed in James Wilson’s lab at the University of Pennsylvania. The large dose of 38 trillion viruses the patient was given sparked massive body-wide inflammation and sent his immune system into overdrive. After that, scientists mostly stopped using adenoviral vectors for gene therapy, in which the dose needs to be high to reach many cells of the body.
** Ryan Cross, “Adenoviral vectors are the new COVID-19 vaccine front-runners. Can they overcome their checkered past?”, Vol. 98, Issue 12, (May 12, 2020)
* It is difficult to make a general judgement about genetic modification (GM), whether vegetable or animal, medical or agricultural, since these vary greatly among themselves and call for specific considerations. The risks involved are not always due to the techniques used, but rather to their improper or excessive application. Genetic mutations, in fact, have often been, and continue to be, caused by nature itself. Nor are mutations caused by human intervention a modern phenomenon. The domestication of animals, the crossbreeding of species and other older and universally accepted practices can be mentioned as examples. We need but recall that scientific developments in GM cereals began with the observation of natural bacteria which spontaneously modified plant genomes. In nature, however, this process is slow and cannot be compared to the fast pace induced by contemporary technological advances, even when the latter build upon several centuries of scientific progress. Although no conclusive proof exists that GM cereals may be harmful to human beings, and in some regions their use has brought about economic growth which has helped to resolve problems, there remain a number of significant difficulties which should not be underestimated.
** Pope Francis Laudato Si’ — On the Care of Our Common Home
* Animals tell us something. If the animals have suffered this way [from GMOs], potentially for us, let’s listen to what they’re telling us. Let’s take heed.
** Jane Goodall, United Nations 2014 Equator Prize Gala, New York City , (September 22, 2014).
* But if the same tests, the same foods are examined by an independent scientist, then it turns out that in almost every case there are quite serious harms done to the rats, the mice or the other poor unfortunate animals, particularly internal organs like liver and kidneys and things of that sort.
** Jane Goodall, United Nations 2014 Equator Prize Gala, New York City , (September 22, 2014).
* Genetic engineering is to traditional crossbreeding what the nuclear bomb was to the sword.
** Andrew Kimbrell, executive director of Center for Food Safety quoted in "Animal Patenting: Impact of Bioengineering on Altering Animals", in B. Julie Johnson E: The Environmental Magazine (Apr 1994).
* So you did do it. You amalgamated one of Godzilla's cells together with the plant's cells. Are you proud of this? What kind science do you call this?
** Kazuhito Kirishima, portrayed by Kunihiko Mitamura in Godzilla vs. Biollante (1989) directed by Kazuki Ōmori
* I stand by my assertions that although you can know what happens to any individual species that you modify, you cannot be certain what will happen to the ecosystem. Also, we have a strange situation where we have malnourished fat [[people]. It’s not that we need more food. It’s that we need to manage our food system better. So when corporations seek government funding for genetic modification of food sources, I stroke my chin.
** Bill Nye in "Bill Nye Explains Why he is a GMO Skeptic", by Keith Kloor, Discover Magazine, (November 6, 2014).
** Bill Nye [NOTE: This position was retracted by Bill Nye less than four months later, per The Washington Post source March 3, 2015, below.]
* I went to Monsasnto, and I spent a lot of time with the scientists there, and I have revised my outlook, and I'm very excited about telling the world. When you’re in love, you want to tell the world.
** Bill Nye Kollipara, Puneet (March 3, 2015). "Proof he’s the Science Guy: Bill Nye is changing his mind about GMOs". The Washington Post. Retrieved on October 15, 2015.
* Everyone is scared of genetic DIY. It's crucial for artists to work with such technologies. It is important that we work between science and art.
** Orlan "Orlan's art of sex and surgery" by Stuart Jeffries, The Guardian, (1 Jul 2009).
* Genetic engineering crosses a fundamental threshold in the human manipulation of the planet, changing the nature of life itself.
** Douglas Parr, scientist and activist, cited in Awake! magazine 2000, 4/22.
* In principle, there's now nothing to stop intelligent moral agents "fixing" the [conditionally-activated level of] subjective physical distress undergone by members of entire free-living species by choosing and propagating benign alleles of SCN9A or its homologs via gene drives, i.e. engineering via CRISPR-mediated gene-editing not a currently utopian "no pain" biosphere ... but a “low pain” biosphere.
** David Pearce, Can Biotechnology Abolish Suffering? (2017), "Compassionate Biology"
* When we wrote Ecofeminism we raised the issue of reductionist, mechanistic science and the attitude of mastery over and conquest of nature as an expression of capitalist patriarchy. Today the contest between an ecological and feminist world-view and a worldview shaped by capitalist patriarchy is more intense than ever. This contest is particularly intense in the area of food. GMOs embody the vision of capitalist patriarchy. They perpetuate the idea of ‘master molecules’and mechanistic reductionism long after the life sciences have gone beyond reductionism, and patents on life reflect the capitalist patriarchal illusion of creation. There is no science in viewing DNA as a ‘master molecule’ and genetic engineering as a game of Lego, in which genes are moved around without any impact on the organism or the environment. This is a new pseudo-science that has taken on the status of a religion. Science cannot justify patents on life and seed. Shuffling genes is not making life; living organisms make themselves. Patents on seed mean denying the contributions of millions of years of evolution and thousands of years of farmers’ breeding. One could say that a new religion, a new cosmology, a new creation myth is being put in place, where biotechnology corporations like Monsanto replace Creation as ‘creators’. GMO means ‘God move over’. Stewart Brand has actually said ‘We are as gods and we had better get used to it.’
** Vandana Shiva in Ecofeminism Maria Mies and Vandana Shiva, 1993, 2014 Foreword (2014)
* I know it's a long shot and people would say it's 'too absurd'… but I'm doing this with hopes of making a Mickey Mouse some day.
** Arikuni Uchimura, quoted in "Japan bio-scientists produce 'singing mouse'"", The Independent, (21 December 2010).
* There is an expression out there that a failed gene therapy makes a good vaccine.
** Luk Vandenberghe as quoted by Ryan Cross in, “Adenoviral vectors are the new COVID-19 vaccine front-runners. Can they overcome their checkered past?”, Chemical & Engineering News, Vol. 98, Issue 12, (May 12, 2020)
* In a more general sense, all the quarter-million plant species— in fact, all species of organisms—are potential donors of genes that can be transferred by genetic engineering into crop species in order to improve their performance. With the insertion of the right snippets of DNA, new strains can be created that are variously cold-hardy, pest-proofed, perennial, fast-growing, highly nutritious, multipurpose, water-conservative, and more easily sowed and harvested. And compared with traditional breeding techniques, genetic engineering is all but instantaneous.
** Edward O. Wilson, The Future of Life (2002), Ch. 5 : How Much Is the Biosphere Worth?

### “Analysis of the Threat of Genetically Modified Organisms for Biological Warfare” (May 2011)

“Analysis of the Threat of Genetically Modified Organisms for Biological Warfare”, Center for Technology and National Security Policy National Defense University, (May 2011)
* Evaluating the potential threat posed by advances in biotechnology, especially genetically modified organisms (GMOs), and synthetic biology remains a contentious issue. The rapid development of the tools of molecular biology and metabolic engineering has enabled the development of chimeric organisms which possess characteristics which are not native to the wild variant. This is commonplace in the area of biomanufacturing, where genes are introduced into organisms such as E coli and products manufactured via large-scale fermentation. More recently, entire metabolic pathways, albeit of limited complexity, have been engineered into organisms, for example, for the production of artemisinin in yeast. In addition to such metabolic engineering projects, whole genomes are being sequenced, leading to the possibility of creating organisms de novo. Numerous lectures, briefings and articles have argued that the dual use nature of biotechnology, the training of foreign students in American universities, and the easy availability of information on the internet have given potential adversaries access to biological weapons of unimagined which pose an existential threat. Some believe that, inevitably, these advances will lead to a catastrophic biological attack. Others have argued the opposite that making all information publicly available will enable a more universal “white biotechnology” which will ultimately monitor the field and provide the means to defeat any threat developed by adversaries. It has been argued that, despite these advances, the scientific and technical requirements, as well as the fundamental laws of natural selection, will prevent such an attack.
** p.5
* Since its first pragmatic elucidation in 1953, DNA structure and genetic engineering has extended its reach into agriculture, animal husbandry, medicine, and even organic materials.
** p.8
* Since the early 1990’s genetically engineered plants have been commercially available. So called “first generation” transgenic plants have been engineered for characteristics that enhance the agricultural yield and marketing. Such characteristics include resistance to pests, herbicides and extreme climates, as well as improved product shelf life. For example, since their first commercial cultivation in 1996, plants have been genetically modified for tolerance to the herbicides glufosinate and glyphosate. A “second generation” of transgenic plants, now in research and development, is aimed at enhancing consumer satisfaction by enhancing taste, texture, or appearance of produce. To date, no second generation transgenic plants are on the market.
** pp.8-9
* Gene therapy, involving the use of viruses as a vector for introducing generic material into cells, has had some success in treating genetic disorders such as severe combined immunodeficiency, and treatments are being developed for a range of other currently incurable diseases such as cystic fibrosis, sickle cell anemia, and muscular dystrophy. Genes introduced in this manner are not transmitted to the next generation. Gene therapy targeting the reproductive cells—so-called “Germ line Gene Therapy”—at present carries an unquantifiable risk associated with interfering with other genes, hence near-term development and commercialization of this technology is unlikely.
** p.9
* The genetic material for modification may be either derived from natural organisms using standard recombinant DNA techniques, or produced by DNA synthesis, the latter being much less labor intensive. In recent work, DNA sequences on the order of 1 million base-pairs have been synthesized entirely from digitized genome sequence information, and the resulting organisms were phenotypical and capable of self-replication.
** p.17
* Development of novel (i.e., not known to be naturally-occurring) GMOs exhibiting unique designer characteristics requires substantially greater knowledge and capability. Many industrialized nations have laboratories capable of analyzing which immune response modifier genes in humans and livestock, when inserted into an organism together with pathogenicity (e.g., adherence and invasive) factor, will yield highly infectious pathogenic organisms.
** p.17

## Dialogue

* Hannah Devlin: In your book you describe a nightmare you had involving Hitler wearing a pig mask, asking to learn more about your “amazing technology”. Do you still have anxiety dreams about where Crispr might leave the human race?
    Jennifer Doudna: I had the Hitler dream and I’ve had a couple of other very scary dreams, almost like nightmares, which is quite unusual for an adult. Not so much lately, but in the first couple of years after I published my work, the field was moving so fast. I had this incredible feeling that the science was getting out way ahead of any considerations about ethics, societal implications and whether we should be worrying about random people in various parts of the world using this for nefarious purposes.
   * Jennifer Doudna, "Jennifer Doudna: ‘I have to be true to who I am as a scientist’", Hannah Devlin, The Guardian, (July 2, 2017).
* Hannah Devlin: In 2015, you called for a moratorium on the clinical use of gene editing. Where do you stand on using Crispr to edit embryos these days?
    Jennifer Doudna: It shouldn’t be used clinically today, but in the future possibly. That’s a big change for me. At first, I just thought why would you ever do it? Then I started to hear from people with genetic diseases in their family – this is now happening every day for me. A lot of them send me pictures of their children. There was one that I can’t stop thinking about, just sent to me in the last 10 days or so. A mother who told me that her infant son was diagnosed with a neurodegenerative disease, caused by a sporadic rare mutation. She sent me a picture of this little boy. He was this adorable little baby, he was bald, in his little carrier and so cute. I have a son and my heart just broke.
   * Jennifer Doudna, "Jennifer Doudna: ‘I have to be true to who I am as a scientist’", Hannah Devlin, The Guardian, (July 2, 2017).
* Snake: I thought using genetically modified soldiers was prohibited by international law.
    Naomi Hunter: Yes, but those are just declarations not actual treaties.
   * Hideo Kojima, Tomokazu Fukushima, Metal Gear Solid, (1998).

## See also

* Jonathan Anomaly
* Scott Alexander
* Cloning
* Eugenics
* Polygenic screening