# Pathogenesis-related protein

Pathogenesis-related (PR) proteins are proteins produced in plants in the event of a pathogen attack. They are induced as part of systemic acquired resistance. Infections activate genes that produce PR proteins. Some of these proteins are antimicrobial, attacking molecules in the cell wall of a bacterium or fungus. Others may function as signals that spread “news” of the infection to nearby cells. Infections also stimulate the cross-linking of molecules in the cell wall and the deposition of lignin, responses that set up a local barricade that slows spread of the pathogen to other parts of the plant.
Salicylic acid plays a role in the resistance to pathogens by inducing the production of pathogenesis-related proteins. Many proteins found in wine are grape pathogen-related proteins. Those include thaumatin-like proteins and chitinases.
Many pathogenesis-related protein families also coincide with groups of human allergens, even though the allergy may have nothing to do with the defense function of the proteins. Grouping these proteins by their sequence features allows for finding potential allergenic proteins from sequenced plant genomes, a field of study dubbed "allergenomics".

## Classification

As of 2014, 17 families of PR proteins have been named:
Different PR-protein families and allergens identified :
| Family | Domain classification | Proteins | Functions | Allergens |
| PR-1 | IPR034111 IPR001283 | PR-1 a, PR-1 b, and PR-1 c | Antifungal (CAP) | Cuc m 3 (muskmelon; P83834)—oral allergy syndrome |
| PR-2 | (GH17) | β-1,3-Glucanases | Cleaves β-1,3-glucans | Hev b 2 (latex; P52407)—contact dermatitis Ole e 9 (olive)—respiratory allergy Mus a 5 (banana)—oral allergy syndrome |
| PR-3 | IPR016283 | Chitinase types I, II, IV, V, VI, and VII | Endochitinase | Pers a 1 (avocado)—itchy eyes or nose, asthma, swelling, and so forth. Mus a 2 (banana)—food allergy like swelling of lips, anaphylaxis, and so forth |
| PR-4 | IPR001153 | Barwin domain chitinase I/II | Antifungal and chitinase | Pro-heveins: Hev b 6—contact dermatitis |
| PR-5 | IPR001938 | Thaumatin-like | Antifungal | Jun a 3 (mountain cedar), Cry j 1 (Japanese cedar), and Cup a 3 (Arizona cypress)—rhinitis, conjunctivitis, and asthma Pru av 2 (cherry), Mal d 2 (apple), Cap a 1 (bell pepper), Act d 2 (kiwi), and Mus a 4 (banana)—oral allergy syndrome |
| PR-6 | IPR000864 | Potato protease I | Proteinase inhibitor | |
| PR-7 | (Subtilisin-like) | Tomato endoproteinase P69 (O82007) | Endoproteinase | |
| PR-8 | (GH18) | Cucumber chitinase | Chitinase III | Hevamine (latex, P23472)—contact dermatitis. Ziz m 1 (Indian jujube, Q2VST0)—oral allergy syndrome Cof a 1 (coffee, D7REL9)—eye and airway allergy |
| PR-9 | (Haem peroxidase III) | Tobacco lignin-forming peroxidase (P11965) | Peroxidase | |
| PR-10 | IPR024949 IPR000916 | Parsley "PR-1" | Ribonuclease-like | Bet v 1 (birch pollen)— allergic rhinoconjunctivitis and asthma Pru av 1 (cherry), Mal  d  1 (apple), Api g 1 (celery), and Dau c 1 (carrot)—oral allergy syndrome Gly m 4 (soy), Vig r 1 (mung bean), Cor a 1 (hazelnut), and Cas s 1 (chestnut), Act c 8 (golden kiwi fruit), Act d 8 (green kiwi fruit) —oral allergy syndrome |
| PR-11 | (GH18) | Tobacco chitinase V (Q43576) | Chitinase | |
| PR-12 | IPR008176 | Radish Rs-AFP3 (O24332) | Plant Defensin | |
| PR-13 | IPR001010 | Arabidopsis THI2.1 (Q42596) | Thionin | |
| PR-14 | IPR000528 | Lipid transfer proteins | Shuttling of phospholipids and fatty acids | Par j 1 (weed; P43217)—rhinitis and asthma Pru p 3 (peach), Mal d 3 (apple), Pru av 3 (cherry), Pru ar 3 (apricot), Cor a 8 (hazelnut), Cas s 8 (chestnut), and Zea m 14 (maize)—oral allergy syndrome |
| PR-15 | IPR001929 | Barley OxOa (P45850) | germin; Oxalate oxidase | |
| PR-16 | IPR001929 | Barley OxOLP (O49871) | germin-like | |
| PR-17 | IPR007541 | Tobacco NtPRp27 (Q9XIY9) | late blight resistance(?) | |

## Identification

As PR proteins are produced when plant tissue is stressed, various ways of stress signaling is used to "bait" the plant into expressing PR genes for identification. Useful stressors include an actual infection or simply defense signals such as salicylate and methyl jasmonate. The proteins can be identified by isolation, peptide digestion, and matching against the genomic sequences (protein sequencing). The sequences obtained can then be checked against known PR protein families for categorization.