# Billboard year-end top 50 R&B singles of 1965

Billboard year-end top 50 R&B singles of 1965 is the year-end chart compiled by Billboard magazine ranking the top rhythm and blues singles of 1965. The list was based on charts from the January 30 through October 30.
The year's No. 1 R&B hit was "I Can't Help Myself (Sugar Pie Honey Bunch)" by the Four Tops which held the No. 1 spot for nine consecutive weeks from June 5 to July 31.
Because the list ends with the October 30 chart, it omits hit songs that peaked after October 30, including (i) Fontella Bass' "Rescue Me" which held the No. 1 spot for four consecutive weeks from October 30 to November 20, and (ii) James Brown's "I Got You (I Feel Good)" which held the No. 1 spot for four consecutive weeks from December 4 to December 25.
| Year-end rank | Weekly peak | Title | Artist(s) | Label |
| 1 | 1 | "I Can't Help Myself (Sugar Pie Honey Bunch)" | Four Tops | Motown |
| 2 | 1 | "In the Midnight Hour" | Wilson Pickett | Atlantic |
| 3 | 1 | "Shotgun" | Junior Walker & The All Stars | Soul |
| 4 | 6 | "I Do Love You" | Billy Stewart | Chess |
| 5 | 2 | "Yes, I'm Ready" | Barbara Mason | Arctic |
| 6 | 1 | "Papa's Got a Brand New Bag" | James Brown | King |
| 7 | 2 | "The Tracks of My Tears" | The Miracles | Tamla |
| 8 | 1 | "We're Gonna Make It" | Little Milton | Checker |
| 9 | 2 | "Tonight's the Night" | Solomon Burke | Atlantic |
| 10 | 1 | "I'll Be Doggone" | Marvin Gaye | Tamla |
| 11 | 3 | "Nothing Can Stop Me" | Gene Chandler | Constellation |
| 12 | 5 | "Don't Mess Up a Good Thing" | Fontella Bass & Bobby McClure | Checker |
| 13 | 1 | "My Girl" | The Temptations | Gordy |
| 14 | 2 | "Stop! In the Name of Love" | The Supremes | Motown |
| 15 | 3 | "Oo Wee Baby, I Love You" | Fred Hughes | VeeJay |
| 16 | 5 | "Nowhere to Run" | Martha and the Vandellas | Gordy |
| 17 | 4 | "Since I Lost My Baby" | The Temptations | Gordy |
| 18 | 13 | "It's a Man Down There" | G. L. Crockett | Brothers |
| 19 | 4 | "Ooo Baby Baby" | The Miracles | Tamla |
| 20 | 5 | "Baby I'm Yours" | Barbara Lewis | Atlantic |
| 21 | 2 | "It's the Same Old Song" | Four Tops | Motown |
| 22 | 1 | "Got to Get You Off My Mind" | Solomon Burke | Atlantic |
| 23 | 1 | "Back in My Arms Again" | The Supremes | Motown |
| 24 | 8 | "Agent Double-O-Soul" | Edwin Starr | Ric Tic |
| 25 | 3 | "It's Growing" | The Temptations | Gordy |
| 26 | 2 | "The 'In' Crowd | Ramsey Lewis Trio | Cadet |
| 27 | 2 | "Shake" | Sam Cooke | RCA Victor |
| 28 | 4 | "Sitting in the Park" | Billy Stewart | Chess |
| 29 | 4 | "Twine-Time" | Alvin Cash & The Crawlers | Mar-V-Lus |
| 30 | 6 | "You're Gonna Make Me Cry" | O. V. Wright | Back Beat |
| 31 | 1 | "I Want To (Do Everything for You) | Joe Tex | Dial |
| 32 | 7 | "Ride Your Pony" | Lee Dorsey | Amy |
| 33 | 7 | "Shake and Fingerpop" | Junior Walker & The All Stars | Soul |
| 34 | 3 | "People Get Ready" | The Impressions | ABC-Paramount |
| 35 | 6 | "I Can't Work No Longer" | Billy Butler | Okeh |
| 36 | 10 | "Mr. Pitiful" | Otis Redding | Volt |
| 37 | | "Unchained Melody" | The Righteous Brothers | Philles |
| 38 | | "Teasin' You" | Willie Tee | Atlantic |
| 39 | | "I've Been Loving You Too Long | Otis Redding | Volt |
| 40 | | "Who's Cheating Who" | Little Milton | Checker |
| 41 | | "Ask the Lonely" | Four Tops | Motown |
| 42 | | "Hurt So Bad" | Little Anthony and the Imperials | DCP |
| 43 | | "You've Lost That Lovin' Feelin'" | The Righteous Brothers | Philles |
| 44 | | "When I'm Gone | Brenda Holloway | Tamla |
| 45 | | "Boo-ga-loo" | Tom & Jerrio | ABC-Paramount |
| 46 | | "Something You Got" | Chuck Jackson & Maxine Brown | Wand |
| 47 | 7 | "I Do" | The Marvelows | ABC-Paramount |
| 48 | 9 | "A Change Is Gonna Come" | Sam Cooke | RCA Victor |
| 49 | 10 | "The Entertainer" | Tony Clarke | Chess |
| 50 | | "The Boy from New York City" | The Ad Libs | Blue Cat |