# 2001 Estoril Open – Women's singles

Anke Huber was the defending champion but lost in the first round to Denisa Chládková.
Ángeles Montolio won in the final 3–6, 6–3, 6–2 against Elena Bovina.

## Seeds

A champion seed is indicated in bold text while text in italics indicates the round in which that seed was eliminated.
* Anke Huber (first round)
* Magdalena Maleeva (second round)
* Justine Henin (semifinals)
* Barbara Schett (second round)
* Magüi Serna (second round)
* Anne Kremer (second round)
* Silvija Talaja (first round)
* Tathiana Garbin (quarterfinals)

## Draw
### Key

* Q = Qualifier
* WC = Wild card
* LL = Lucky loser
* Alt = Alternate
* SE = Special exempt
* PR = Protected ranking
* ITF = ITF entry
* JE = Junior exempt
* w/o = Walkover
* r = Retired
* d = Defaulted
* SR = Special ranking
| First round | First round | First round | First round | First round | Second round | Second round | Second round | Second round | Second round | Quarterfinals | Quarterfinals | Quarterfinals | Quarterfinals | Quarterfinals | Semifinals | Semifinals | Semifinals | Semifinals | Semifinals | Final | Final | Final | Final | Final |
| 1 | A Huber | 5 | 6 | 2 | | | | | | | | | | | | | | | | | | | | |
| | D Chládková | 7 | 3 | 6 | | D Chládková | 6 | 4 | 6$^5$ | | | | | | | | | | | | | | | |
| | R Grande | 6 | 6 | | | R Grande | 1 | 6 | 7$^7$ | | | | | | | | | | | | | | | |
| | M Vavrinec | 4 | 0 | | | | | | | | R Grande | 3 | 4 | | | | | | | | | | | |
| | R Kuti-Kis | 3 | 4 | | | | | | | | Á Montolio | 6 | 6 | | | | | | | | | | | |
| | Á Montolio | 6 | 6 | | | Á Montolio | 6 | 6 | | | | | | | | | | | | | | | | |
| | A Gerši | 4 | 7$^7$ | 4 | 6 | A Kremer | 2 | 2 | | | | | | | | | | | | | | | | |
| 6 | A Kremer | 6 | 6$^2$ | 6 | | | | | | | | | | | | Á Montolio | 5 | 7$^7$ | 6 | | | | | |
| 3 | J Henin | 7$^7$ | 4 | 6 | | | | | | | | | | | 3 | J Henin | 7 | 6$^4$ | 4 | | | | | |
| | A Medina Garrigues | 6$^3$ | 6 | 4 | 3 | J Henin | 6 | 6 | | | | | | | | | | | | | | | | |
| Q | L Bacheva | 2 | 2 | | | M Marrero | 4 | 3 | | | | | | | | | | | | | | | | |
| | M Marrero | 6 | 6 | | | | | | | 3 | J Henin | 6 | 6 | | | | | | | | | | | |
| | S Plischke | 4 | 1 | | | | | | | | F Schiavone | 2 | 4 | | | | | | | | | | | |
| | F Schiavone | 6 | 6 | | | F Schiavone | 6 | 6 | | | | | | | | | | | | | | | | |
| | S Pitkowski | 6 | 6 | | | S Pitkowski | 1 | 2 | | | | | | | | | | | | | | | | |
| 7 | S Talaja | 2 | 3 | | | | | | | | | | | | | | | | | | Ángeles Montolio | 3 | 6 | 6 |
| 5 | M Serna | 6 | 6 | | | | | | | | | | | | | | | | | Q | Elena Bovina | 6 | 3 | 2 |
| | B Rittner | 3 | 4 | | 5 | M Serna | 3 | 6 | 1 | | | | | | | | | | | | | | | |
| Q | E Bovina | 6 | 7 | | Q | E Bovina | 6 | 2 | 6 | | | | | | | | | | | | | | | |
| Q | D Randriantefy | 3 | 5 | | | | | | | Q | E Bovina | 4 | 7 | 6 | | | | | | | | | | |
| WC | A C Nogueira | 3 | 5 | | | | | | | | T Pisnik | 6 | 5 | 4 | | | | | | | | | | |
| | T Pisnik | 6 | 7 | | | T Pisnik | 7$^7$ | 6 | | | | | | | | | | | | | | | | |
| WC | F Piedade | 2 | 0 | | 4 | B Schett | 6$^5$ | 1 | | | | | | | | | | | | | | | | |
| 4 | B Schett | 6 | 6 | | | | | | | | | | | | Q | E Bovina | 7 | 6 | | | | | | |
| 8 | T Garbin | 2 | 6 | 6 | | | | | | | | | | | | J Kandarr | 5 | 3 | | | | | | |
| | T Poutchek | 6 | 1 | 2 | 8 | T Garbin | 6 | 6 | | | | | | | | | | | | | | | | |
| | K Habšudová | 2 | 4 | | Q | J Husárová | 4 | 1 | | | | | | | | | | | | | | | | |
| Q | J Husárová | 6 | 6 | | | | | | | 8 | T Garbin | 5 | 2 | | | | | | | | | | | |
| | J Kandarr | 6 | 7$^7$ | | | | | | | | J Kandarr | 7 | 6 | | | | | | | | | | | |
| | K Hrdličková | 2 | 6$^5$ | | | J Kandarr | 6 | 7 | | | | | | | | | | | | | | | | |
| | E Gagliardi | 5 | 4 | | 2 | M Maleeva | 3 | 5 | | | | | | | | | | | | | | | | |
| 2 | M Maleeva | 7 | 6 | | | | | | | | | | | | | | | | | | | | | |