# Pieter Cullis

Pieter Rutter Cullis FRS FRSC OBC OC is a Canadian physicist and biochemist known for his contributions to the field of lipid nanoparticles (LNP). Cullis and co-workers have been responsible for fundamental advances in the development of nanomedicines employing lipid nanoparticle (LNP) technology for cancer therapies, gene therapies and vaccines. This work has contributed to five drugs that have received clinical approval by the US Food and Drug Agency (FDA), the European Medicines Agency, and Health Canada.
Cullis has also co-founded eleven biotechnology companies that now employ over 400 people, has published over 400 scientific articles (h-index 138) and is an inventor on over 100 patents. Companies he has co-founded include Acuitas Therapeutics, Integrated Nanotherapeutics, Precision NanoSystems, and NanoVation Therapeutics. He has also co-founded and has been the Founding Scientific Director of two National Centre of Excellence networks: the Centre for Drug Research and Development (now AdMare) in 2004 and the NanoMedicines Innovation Network in 2019. These not-for-profit networks are aimed at translating basic research in the life sciences into commercially viable products.
Two recently approved drugs that are enabled by LNP delivery systems devised by Cullis, members of his UBC laboratory, and colleagues in the companies he has co-founded deserve special emphasis. The first is Onpattro which was approved by the US FDA in August 2018 to treat the previously fatal hereditary condition transthyretin-induced amyloidosis (hATTR). Onpattro is the first RNAi drug to receive regulatory approval. The second is Comirnaty, the COVID-19 mRNA vaccine developed by Pfizer/BioNTech that has received regulatory approval in many jurisdictions including Canada, the USA, the UK and Europe. Comirnaty has played a major role in containing the global COVID-19 pandemic with approximately 6B doses administered worldwide in 2021 and 2022.

## Research Interests

Cullis's research interests concern the roles of lipids in biological membranes and the development of nanomedicines using lipid nanoparticle (LNP) technology. His studies on the roles of lipids focus on two areas. The first concerns the ability of membrane lipids to adopt non-bilayer structures and the roles of such structures in processes such as membrane fusion. The second area focuses on transport across bilayer lipid systems induced by trans-bilayer ion gradients. His interests in nanomedicines first concern the design and synthesis of LNP delivery systems containing small molecule drugs, particularly drugs used in cancer chemotherapy, with the aim of increasing potency and reducing toxicity by enhancing drug delivery to, and release at, sites of disease such as tumours. A second area involves using LNP technology to enable the therapeutic use of macromolecular genetic drugs such as small interfering RNA (siRNA) and messenger RNA (mRNA) for gene therapy, including gene editing. These efforts have led to five nanomedicines that have been approved for clinical use by regulatory agencies such as the US Food and Drug Agency (FDA), the European Medicines Agency and Health Canada (see Table below).
Two of these nanomedicines were recently approved and are of particular note. First is the drug Onpattro, a gene therapy that was approved (August 2018) by the FDA to treat hereditary amyloid transthyretin (hATTR) amyloidosis. Onpattro is the first-in-class RNAi-based drug to be approved by the FDA and employs an LNP delivery system developed in collaboration with Alnylam Pharmaceuticals (Boston), his laboratory at UBC and two spin-offs that he co-founded (Protiva/Arbutus and Acuitas Therapeutics). Onpattro delivers an siRNA to silence the TTR gene in the liver. A remarkable feature of Onpattro is that it is able to not only stop further progression of this hitherto untreatable disease (which usually leads to death within five years of diagnosis), but also to reverse the neuropathies and cardiovascular issues associated with hATTR. The second class of medicines are the mRNA Covid 19 vaccines that Acuitas developed with Pfizer/BioNTech and CureVac. The Pfizer/BioNTech COVID-19 mRNA vaccine BNT162b2 relies on an LNP delivery system developed by Acuitas. BNT162b2 (Comirnaty) was approved for emergency use (December 2020) by the US FDA and has subsequently been approved by the US, UK, Canada, Europe and many other countries worldwide. These approvals were made on the basis of a 95% efficacy in preventing COVID-19, together with an excellent safety profile. More than five billion doses of this vaccine were administered globally in 2021-22 and the vaccine has been credited with saving over six million lives in 2021 alone.

## Nanomedicine Drugs

The following approved nanomedicine drugs have been co-developed by Cullis's laboratory at the University of British Columbia and by the companies he has co-founded (Inex Pharmaceuticals Corp, Protiva Biotherapeutics, Canadian Liposome Company, Acuitas Therapeutics).
| Nanomedicine Drug | Trade Name | Indication | Status (2020) | Company |
| LNP Amphotericin | Abelcet | Fungal infections | Approved US, Europe - 1995 | Enzon/Canadian Lipo Co |
| LNP Doxorubicin | Myocet | Metastatic breast cancer | Approved Europe, Canada - 2000 | Cephalon/Canadian Lipo Co |
| LNP Vincristine | Marqibo | Acute lymphoblastic leukemia | Approved US - 2012 | Spectrum Pharma/Inex |
| LNP siRNA TTR | Onpattro | Transthyretin-induced amyloidosis | Approved US, Europe - 2018 | Alnylam/Acuitas |
| LNP mRNA SARS Cov 2 | Comirnaty | COVID-19 mRNA vaccine | Approved US, Europe - 2021 | Pfizer/BioNTech/Acuitas |

## Research and Professional Experience

| Institution/Company | Title |
| UBC, Department of Biochemistry & Molecular Biology | Assistant Professor (1978); Associate Professor (1982); Professor (1985-present) |
| Lipex Biomembranes (now Evonik Canada) | Co-Founder (1985); Chairman (1985 -2000) |
| Canadian Liposome Company | Co-Founder (1986); Director & President (1986-1991) |
| Northern Lipids (now Evonik Canada) | Co-Founder (1992); Director (1992-1996) |
| Inex Pharmaceuticals Corp (now GeneVant) | Co-Founder (1992); Director (1992-2007); VP Research (1992-2004) |
| Protiva Biotherapeutics (now Arbutus) | Co-founder (2001); Director & Chair, SAB (2001-2005) |
| Centre Drug Research & Development (now AdMare) | Co-founder (2004); Scientific Director (2004-2010) |
| Acuitas Therapeutics | Co-Founder (2009); Chairman (2009-2021) |
| Precision NanoSystems | Co-Founder (2010); Chairman (2010-2015) |
| Personalized Medicine Initiative | Co-Founder (2011); Chairman (2011-2017) |
| Life Sciences Institute, UBC | Director (2013-2017) |
| GenXys Health Care Systems | Co-Founder (2014); Director (2014-2016) |
| Mesentech | Co-Founder (2014); Director (2014-2019) |
| Molecular You Corporation | Co-Founder (2014); Chairman (2014-present) |
| AllerGen National Centre of Excellence | Director (2014-present); Chairman (2017-present) |
| Integrated NanoTherapeutics | Co-Founder (2015); Chairman (2015-2021) |
| NanoMedicines Innovation Network NCE | Founder (2019); Scientific Director & CEO (2019-2021) |
| NanoVation Therapeutics | Co-Founder (2020); Chairman (2020-present) |

## Academic and Professional Awards

| Start | End | Name of Award | Agency |
| 1973 | 1976 | MRC Postdoctoral Fellowship | Canadian Medical Research Council |
| 1977 | 1977 | EMBO Postdoctoral Fellowship | European Molecular Biology Organization |
| 1978 | 1982 | MRC Scholar | Medical Research Council of Canada |
| 1983 | 1989 | MRC Scientist | Medical Research Council of Canada |
| 1986 | | Ayerst Award | Canadian Biochemical Society |
| 1988 | 1989 | UBC Killam Research Prize | University of British Columbia |
| 1988 | | Jacob Bierly Research Prize | University of British Columbia |
| 1991 | | Gold Medal for Health Sciences | BC Science Council |
| 2000 | | Alec Bangham Award | International Liposome Society |
| 2002 | | Award for Innovation and Achievement | BC Biotechnology Alliance |
| 2004 | | Fellow of the Royal Society of Canada | Royal Society of Canada |
| 2005 | | Barre Award | University of Montreal |
| 2005 | | UBC Alumni Award for Research, Science & Medicine | University of British Columbia |
| 2010 | | CSPS Leadership Award | Canadian Society for Pharmaceutical Sciences |
| 2011 | | Bill and Marilyn Webber Lifetime Achievement Award | University of British Columbia Faculty of Medicine |
| 2011 | | Prix Galien Canada Research Award | Prix Galien Canada |
| 2012 | | Senior Faculty Award-Outstanding Contributions | University of British Columbia Faculty of Medicine |
| 2015 | | Milton Wong Leadership Award | Life Science BC |
| 2016 | | Lifetime Achievement Award | Journal of Drug Targeting |
| 2018 | | Fellow National Academy of Inventors | National Academy of Inventors, USA |
| 2021 | | Founder’s Award | Controlled Release Society, USA |
| 2021 | | Prince Mahidol Award (Medicine) | Prince Mahidol Award Foundation, Thailand |
| 2021 | | Officer, Order of Canada | Governor General of Canada |
| 2022 | | VinFuture Prize, Grand Prize | VinFuture Foundation, Vietnam |
| 2022 | | Canada Gairdner International Award | Gairdner Foundation, Canada |
| 2022 | | Lipid Science Prize | Camurus Foundation, Sweden |
| 2022 | | Thudichum Life Award | Phospholipid Research Centre, Germany |
| 2022 | | UBC Alumni Award of Distinction | University of British Columbia |
| 2022 | | Tang Prize (Biopharmaceutical Science) | Tang Foundation, Taiwan |
| 2022 | | Governor General’s Innovation Award | Rideau Hall Foundation, Canada |
| 2022 | | Global Impact Award | Life Sciences BC |
| 2022 | | Bloom Burton Award | Bloom Burton & Co. |
| 2023 | | Julia Levy Award | Society for Chemical Industry |
| 2023 | | Doctorate Honoris Causa | Ghent University, Belgium |
| 2023 | | Killam Prize | Killam Foundation, National Research Council, Canada |
| 2023 | | Fellow of the Royal Society | Royal Society, London |
| 2023 | | Sultan Karim Medal of Excellence in Clinical Pharmacology | University of British Columbia Faculty of Medicine |
| 2023 | | Member, Order of British Columbia | Governor General of BC |
| 2023 | | Member, Canadian Medical Hall of Fame | Canadian Medical Hall of Fame |
| 2023 | | Harvey Prize (awarded for the year 2021) | Technion, Israel |
| 2023 | | Bill and Marilyn Webber Lifetime Achievement Award | University of British Columbia Faculty of Medicine |
| 2024 | | Maurice-Marie Janot Award | Association de Pharmacie Galénique Industrielle, France |