# U.S. Route 27 in Florida

U.S. Highway 27 (US 27) in Florida is a north–south United States Numbered Highway. It runs 496.352 miles (798.801 km) from the Miami metropolitan area northwest to the Tallahassee metropolitan area. Throughout the state, US 27 has been designated the Claude Pepper Memorial Highway by the Florida Legislature. It was named after long-time Florida statesperson Claude Pepper, who served in both the U.S. Senate and House of Representatives. The stretch running from Miami to South Bay was originally designated the Thomas E. Will Memorial Highway by the Florida Legislature in 1937 when that portion was known as State Road 26 (SR 26). Thomas E. Will, the founder of Okeelanta, had worked for almost 20 years to get the state to build a road from Miami to the area south of Lake Okeechobee. For most of its length in the state, US 27 is a divided highway.
Between Miami and Ocala, US 27 follows SR 25; between Leesburg and Williston, it follows SR 500; between Williston and High Springs, it follows SR 45; between High Springs and downtown Tallahassee, it follows SR 20; within downtown Tallahassee, it follows SR 61; and, between Tallahassee and the Georgia border, it follows State Road 63 (SR 63).
Concurrencies include SR 80 between South Bay and Clewiston, SR 78 from Moore Haven to Citrus Center, US 98 between Sebring and western Frostproof, and US 441 between Leesburg and Ocala, which also includes a concurrency with US 301 between Belleview and Ocala. Others include US 41 between Williston and High Springs, SR 20 between High Springs and Tallahassee, US 129 in Branford, US 19 between Perry and Capps, and SR 61 in Tallahassee.

## Route description
### Miami through Central Florida

US 27 begins its northward journey as North 36th Street in Midtown Miami, heading west from US 1 for 4.4 miles (7.1 km) before turning northwest to pass under the western terminus of SR 112 (Airport Expressway), while also passing under Interstate 95 (I-95) without a direct interchange. It then proceeds northwest for five miles (8.0 km) as South Okeechobee Road, parallel to the Miami Canal, forming the southwest boundary of the city of Hialeah. After an interchange with SR 826 (Palmetto Expressway), it continues northwest as North Okeechobee Road for five miles (8.0 km) before an interchange with the Homestead Extension of the Florida Turnpike. After another four miles (6.4 km), the highway curves to the north and, after passing the northern terminus of SR 997 (Krome Avenue), crosses into Broward County.
In Broward County, the highway passes protected wetlands and heavy-duty powerlines on the west and the outer reaches of the suburban communities of Pembroke Pines and Weston on the east where it curves to the northwest. It passes by West Broward High School in Pembroke Pines, in which many school busses coming from SR 822 (Sheridan Street) use US 27 to get to Johnson Street to where the high school is. US 27 then reaches an interchange with I-75 and Alligator Alley (exit 23) which is an elaborate partial cloverleaf interchange with flyovers from US 27, loop ramps from I-75, and no reentry to either road. From here, the road is surrounded by Everglades-related wilderness and recreational areas before curving to the north toward South Bay, where it intersects SR 80 and overlaps the road before curving west along the shores of Lake Okeechobee. The highway skirts the southwestern shore of Lake Okeechobee and then heads west through Lake Harbor and then Clewiston, before making a sharp turn to the north toward Moore Haven, where it crosses the Mamie Langdale Memorial Bridge over the Caloosahatchee Canal and makes another sharp turn to the west. The road then intersects with SR 78, which it overlaps until reaching Citrus Center, then proceeds in a northerly direction toward the central Florida communities such as Lake Placid, where it intersects SR 70.
The southern terminus of the concurrency with US 98 is also the eastern terminus of SR 66. From here, the hidden routes are SR 25 and SR 700. Shortly after this, US 27/US 98 runs through a commercial strip area before curving to the west at a Y intersection along the south shore of Lake Jackson in Sebring, where SR 17 begins. The road heads back to the northwest as it runs along and then away from the edge of the lake. North of here, the road runs west of Lake Sebring, but, in Avon Park, it runs much closer to the shores of Lake Glenada, where it passes through South Florida State College territory. From here, it passes by lakes Lelia and Anoka, and, just east of Avon Park Executive Airport, it intersects SR 64 and the northern terminus of a segment of SR 17. SR 64 continues east along part of SR 17 as a bi-county extension northeast into Polk County which runs through the Lake Wales Ridge State Forest and terminates at the Avon Park Air Force Range. North of here, US 27 carries hidden state routes (SR 17, SR 25, and SR 700) into Polk County until it reaches Sunray Deli Estates, where SR 17 breaks away again, and runs relatively parallel to US 27 until it reaches Haines City. Meanwhile, after making a reverse curve over a bridge above a CSX Transportation railroad line that is used by Amtrak's Silver Star and Silver Meteor lines, US 98 breaks away in western Frostproof, taking SR 700 with it. In the opposite direction of this intersection is a continuation of County Road 630 (CR 630). After passing by Warner University, Crooked Lake Park, and CR 640, US 27 becomes slightly less rural as it approaches an unnumbered partial cloverleaf interchange with SR 60 in Lake Wales. North of this point, US 27 becomes a six-lane highway and remains that way until reaching SR 540 in Waverly, where the road narrows down to four lanes again. The widening of US 27 to a six-lane highway, however, continues in Polk County between here and SR 542 in Dundee. North of here, US 27 runs through Lake Hamilton and curves around the eastern shores of the lake for which the community was named. After passing by a pair of gated communities and crossing over a bridge between Middle Lake Hamilton, and the ironically named larger Little Lake Hamilton, it curves north and intersects SR 544. Taking a turn to the northeast after passing by Lake Henry, US 27 encounters another unnumbered interchange with US 17/US 92 in Haines City and immediately passes over another CSX Transportation railroad line that is used by Amtrak's Silver Star and Silver Meteor lines. With the exception of a segment between Hammock Lake and Tower Lake, most of US 27 remains a commercial strip, especially as it enters Davenport, and approaches quarter-cloverleaf interchanges on the southeast and northwest corners of the bridge over I-4 at exit 55.

### Citrus Ridge to Santos

North of I-4, US 27 contains more unnumbered interchanges. The first of which is a trumpet interchange with US 192 (SR 530) on the Polk–Lake county line in Citrus Ridge, and, shortly afterward, an at-grade intersection with CR 474, although some maps have indicated another interchange here. Most of the road remains a rural four-lane highway with at-grade intersections. After passing by Lake Louisa State Park and winding around the eastern shores of the lake that the park was named for, US 27 approaches a cloverleaf interchange without exit numbers at SR 50 in Clermont. After passing the Florida Citrus Tower and Presidents Hall of Fame, the road enters Minneola along the east side of Lake Minneola, where it crosses a bridge over South Lake Trail, intersects such roads as CR Old 50, as well as CR 561, the latter of which it shares a brief concurrency. After this section, it briefly runs parallel to the southwest side of Florida's Turnpike, until it veers slightly to the west and approaches a quarter-cloverleaf interchange with SR 19 south of Howey-in-the-Hills, which also includes a southbound interchange with Florida's Turnpike. The northbound turnpike interchange can be found further northwest. At Okahumpka, US 27 intersects two county roads that are extensions of state roads. The first of which is CR 48, and the next of which is the northern terminus of CR 33 before running along the western shore of Lake Harris and eastern shore of Lake Denham, the location of Singletary Par also known as the Leesburg Fishing Area. Here, it intersects CR 25A. North of Lake Harris, it enters Leesburg where US 27 has an intersection with SR 44. US 441 later joins US 27, and, as a result, the hidden state routes are officially SR 25/SR 500. SR 500 becomes more prominent however, because in Lady Lake, SR 25 branches away as CR 25. This once served as the southern terminus of the US 27/US 441 Alternate (US 441 Alt.) around Lake Weir into Belleview but still remains a scenic route.
Around the Lake–Marion county border, US 27/US 441 enters a retirement community known as The Villages. Because of the frequent use of street-legal golf cars in the community, bridges and tunnels for these carts can be found throughout the road. US 27/US 441 leaves The Villages as it approaches CR 42, a bi-county east–west scenic route through Central Florida. North of here, it serves as the newly constructed bypass that leads to CR 484, in Summerfield. Within Belleview, the road encounters two somewhat important intersections; CR 25A and then SR 35. SR 35 joins US 27/US 441 in a wrong-way concurrency with SR 500 as the road curves more to the west, until it reaches the intersection with US 301, where SR 35 turns south, and SR 500 becomes the hidden state road for US 27/US 301/US 441 for a few blocks. SR 25, however, reunites with the triple concurrency at the former eastern terminus of CR 484, and shares a concurrency with SR 25, and US 27/US 301/US 441's new secret route becomes SR 25/SR 500. After that section, the road curves more to the north again. Near the right-of-way for the formerly proposed Cross Florida Barge Canal, the median for the road widens but narrows back down to normal again. Before it does, however, it intersects CR 328, where a police station exists between the median. Supports for a bridge that was intended to run over the canal exist behind the police station. To the west of this intersection is the Santos Trailhead of the Marjorie Harris Carr Cross Florida Greenway.

### Santos through High Springs

Before US 27/US 301/US 441 enters Ocala, it veers off to the left at an intersection with CR Old 441 (Southeast Lake Weir Avenue), a former segment of US 441 that eventually leads to the Ocala Union Station. The first major intersection after this section is 31st Street. Then, it crosses under a railroad bridge before reaching the city limits and the intersections with the northern terminus of CR 475 and crossing SR 464 (17th Street). US 27/US 301/US 441 intersects with SR 200 (becoming the new hidden state road until US 301 reaches US 1/US 23). The highway reaches the heart of Ocala at the intersection with SR 40 (West Silver Springs Boulevard). After this section, the road crosses a railroad bridge west of Ocala Union Station. Five blocks later, it reaches the intersection of SR 492 (Northwest 10th Street) only to move in the opposite direction, leaving the US 301/US 441 overlap and taking SR 500 with it. After retaining its status as an independent U.S. Highway, US 27 moves from Northwest Tenth Street to Northwest Blitchton Road and has another interchange with I-75 at exit 354, although this one is a mere diamond interchange, as opposed to the elaborate partial cloverleaf interchange with flyovers in Weston. From here, it continues northwest through rural Marion County, much of which is surrounded by horse farms. The few truly significant intersection in this region include CR 464B in Fellowship and then CR 326 near Emathla.
The last intersections at the Marion–Levy county line are with CR 316 and CR 335. Though horse farms are scarce along this segment in Levy County, the surroundings remain rural. CR 316 begins again at an intersection with US 27 in East Williston, and as the road enters the city of Williston, it serves as the western terminus of CR 318, crosses a set of CSX Transportation railroad tracks, and loses its status as an independent U.S. Highway. SR 500 continues straight ahead along US 27 Alt., while US 27 turns north and joins US 41 (hidden SR 45), as well as SR 121, which already ran east for a few blocks along US 27 Alt. The concurrency with SR 121 is short-lived though, as it branches away to the northeast. The US 27/US 41 concurrency is one of the few segments that are only two lanes wide, and it begins to lean to the northwest until briefly shifting straight north before approaching Archer, where it intersects CR 346, then a former Florida Central and Peninsular Railroad right-of-way, and finally SR 24. After SR 24, US 27/US 41 curves back northwest but heads straight northward through SR 26 in Newberry, and both remain as such until US 41 reaches High Springs, joins US 441 toward Lake City, and points north.

### High Springs to Tallahassee

US 27 heads west along unsigned SR 20, which is named First Avenue until it crosses the Santa Fe River and enters Columbia County. From here, it continues to run northwest but briefly turns straight west after entering Fort White just before intersecting SR 47. The road shifts to the right again and turns northwest before leaving the city but gradually turns back west as it gets closer to Suwannee County. Before the road crosses the Ichetucknee River at the Columbia–Suwannee county line, it intersects with Southwest Riverside Avenue, which serves as the western terminus of the O'Leno to Ichetucknee Trail and the eastern terminus of the Suwannee River Greenway, both of which are along a former Atlantic Coast Line Railroad line. Both US 27 and the trail run through Hildreth, the home of Ichetucknee Springs State Park, and then encounters the east end of a concurrency with US 129 (SR 49) which is also the southern terminus of CR 49. US 27/US 129 and the trail enter the hamlet of Wachtokha, but the trail remains straight while the road curves slightly to the northwest. A slight curve to the southwest is where both routes officially enter Branford where US 129 turns north onto unsigned SR 249. The Suwannee River Greenway crosses US 27 between the west end of the US 27/US 129 concurrency and Frank R. Norris Bridge across the Suwannee River, but it was also once the northern terminus of US 129 Alt., which followed US 27 across the river into Lafayette County until it reached SR 349 in Grady.
The road runs through the rural community of Alton, then slows down as it enters Mayo where it intersects SR 51, the northwest corner of which contains the historic Lafayette County Courthouse. After the intersection of Lafayette Street, the road curves northwest again. Outside the city limits, the road intersects various local roads, the most significant of which is CR 534. The rest of the way, US 27 runs northwest through more of western rural Lafayette County, but it turns straight west again as it approaches Buckville where it intersects the southern terminus of CR 53 and northern terminus of CR 534, both of which were once part of SR 53. The last intersection in Lafayette County is with CR 348 in Townsend before US 27 becomes surrounded by swampland as it enters Taylor County.
Swampland continues to surround US 27 until it comes close to Perry, where a small truck weigh station can be found along the westbound shoulder. The road divides momentarily at the intersection of SR 30, which has controlled turn lanes in the divider, but then turns back into an undivided two lane road as it curves northwest. At Buckeye Nursery Road, US 27 gets the name East Hampton Springs Avenue. The avenue curves from northwest to southwest once again and then straight west at Washington Street. The street name changes from East Hampton Springs Avenue to West Hampton Springs Avenue at US 221 (hidden SR 55) and also joins US 221 Truck. Several blocks later, the road intersects Byron Butler Parkway which is the northern terminus of the US 19/US 98/US 27 Alt. concurrency. Here, US 27 Alt. terminates, US 98 and SR 30 leave US 19 turning west onto West Hampton Springs Avenue, and US 27 joins US 19 becoming a four-lane highway again. US 19/US 27 also continues to carry US 221 Truck until it reaches CR 359A (Wright Road) but also continues to take SR 20 with it heading straight northwest through the rest of Taylor County and then very briefly in Madison County, where it intersects CR 150. US 19 breaks away in Capps onto SR 57 on its way through Monticello, but not before resuming a westward direction at the Madison–Jefferson county line. In Tallahassee, the road becomes the 1957-built Apalachee Parkway, a major east–west thoroughfare. The parkway has a short expressway section just east of the capitol, then is a busy four-lane surface boulevard with service roads for the next few miles, passing Governor's Square, the Centre of Tallahassee, and many state office buildings. US 27 runs west along this stretch of road however, and the Apalachee Parkway ends at SR 61 (Monroe Street) in front of the Florida State Capitol building. US 27 and SR 20 make a sharp turn north along SR 61, but, at the intersection of US 90, SR 20 turns west while US 27 continues north along SR 61.

### North from Tallahassee

After the intersection with US 90, hidden SR 63 officially begins at the intersection of North Monroe Street and Thomasville Road, where SR 61 resumes its status as a separate exposed route. After crossing Seventh Avenue, it begins to curve to the northwest avoiding Lake Ella. The first intersection during this curve is CR 158 (Tharpe Street) and is the beginning of SR 63's alternate street name of Old Quincy Highway.
SR 63 approaches its only suffixed auxiliary route in the form of CR 63A before encountering a partial cloverleaf interchange with I-10 (exit 199). North of the interchange, it begins to leave the Tallahassee city limits. A great deal of this segment of SR 63 consists of RV parks and other facilities serving tourists for Lake Jackson Mounds Archaeological State Park. It also serves as the northeast terminus of CR 356. After crossing a bridge that runs over a small portion of Lake Jackson, the road simultaneously intersects the northern terminus of SR 263 and southern terminus of CR 157 and CR 0361.
The road runs along the west side of Tallahassee Commercial Airport and makes a reverse curve as it approaches a bridge over the Ochlockonee River, where it crosses the Leon–Gadsden county line and continues to move northwest. After crossing another bridge, the first major intersection in the county is the eastern terminus of CR 270. Further north, CR 159 secretly joins US 27/SR 63 in a triple concurrency, and all three routes approach a CSX Transportation railroad line until they curve northeast before entering the town of Havana. CR 159 branches off on its own to the northwest before US 27/SR 63 curves straight north as it approaches the intersection with SR 12 and CR 12A. SR 12 joins the road in a brief concurrency before branching off to the east as CR 12, within the Havana town limits. The previously mentioned CSX Transportation railroad line runs more or less parallel to the road again. The road and the tracks curve back and forth near each other until the tracks take one final turn to the northwest in Hinson, and SR 63 begins to curve back to the northeast before the intersection with CR 159A. Shortly after this section, SR 63 curves further to the northeast as it approaches CR 12B. Finally in Darsey, SR 63 serves as the northern terminus of CR 157 and the southern terminus of an extension of State Route 111 (SR 111), before curving to the northwest again and finally terminating at the Florida–Georgia state line, where US 27 continues onto SR 1.

## History

US 27 first entered Florida in 1934, terminating at Tallahassee. In 1949, it was extended to its current terminus in Miami.
From 1956 until 1993, US 27 signs in Florida featured white numbering on a green shield. The "color-coding" of U.S. Highways by the Florida Department of Transportation was stopped when the state could no longer use federal funds to replace the signs with anything but the standard black-and-white version; a few green US 27 signs remain.
Shortly after its second landfall, Hurricane Irma's eye traveled roughly along the entire course of US 27 in Florida north of Lake Placid.
In January 2021, Representative Anthony Sabatini announced he will sponsor an amendment to name the section of US 27 in Florida as the President Donald J. Trump Highway after former President Donald Trump.

## Major intersections

| County | Location | mi | km | Destinations | Notes |
| Miami-Dade | Miami | 0.000 | 0.000 | US 1 (Biscayne Boulevard / SR 5) to I-95 / I-195 – Miami Beach | I-195 exit 2B; southern end of SR 25 concurrency |
| Miami-Dade | Miami | 1.138 | 1.831 | US 441 (Northwest 7th Avenue / SR 7) | |
| Miami-Dade | Miami | 1.395 | 2.245 | Northwest 10th Avenue to Express Lanes north | |
| Miami-Dade | Miami | 1.651 | 2.657 | SR 933 (Northwest 12th Avenue) | |
| Miami-Dade | Miami | 2.660 | 4.281 | To SR 112 / I-95 / Northwest 22nd Avenue | |
| Miami-Dade | Miami | 3.171 | 5.103 | SR 9 (Northwest 27th Avenue) to SR 112 / I-95 – Airport | |
| Miami-Dade | Hialeah | 4.405 | 7.089 | SR 948 west (Northwest 36th Street) | |
| Miami-Dade | Hialeah | 4.43 | 7.13 | SR 112 east to I-95 – Miami Beach | Southbound exit and northbound entrance |
| Miami-Dade | Hialeah | 4.735 | 7.620 | SR 953 (LeJeune Road) – Miami International Airport | |
| Miami-Dade | Hialeah | 6.018 | 9.685 | SR 944 east (Northwest 54th Street / Hialeah Drive) / Curtiss Parkway | |
| Miami-Dade | Hialeah | 6.785 | 10.919 | SR 823 north (Red Road / West 4th Avenue) | No access from US 27 south |
| Miami-Dade | Hialeah–Hialeah Gardens line | 9.43 | 15.18 | SR 826 – Airport | Interchange |
| Miami-Dade | Hialeah Gardens | 10.844 | 17.452 | SR 932 east (Northwest 103rd Street) | |
| Miami-Dade | Hialeah Gardens | 12.159 | 19.568 | To I-75 / Hialeah Gardens Boulevard / Northwest 116th Way | |
| Miami-Dade | Hialeah Gardens | 14.489 | 23.318 | Florida's Turnpike Extension (SR 821) – Orlando, Homestead | Turnpike exit 35 |
| Miami-Dade | ​ | 19.247 | 30.975 | SR 997 south (Krome Avenue) – Homestead, Key West | Former SR 27 south |
| Broward | Pembroke Pines | 23.218 | 37.366 | SR 820 east (Pines Boulevard) to I-75 | |
| Broward | Pembroke Pines | 24.720 | 39.783 | To SR 822 / I-75 / Sheridan Street | |
| Broward | Southwest Ranches | 26.776 | 43.092 | To SR 818 / I-75 / Griffin Road – Everglades Holiday Park | |
| Broward | Weston | 32.65 | 52.55 | I-75 (Alligator Alley / SR 93) to SR 84 east – Miami, Fort Lauderdale, Naples | I-75 exit 23 |
| Palm Beach | Okeelanta | 69.691 | 112.157 | CR 827 | |
| Palm Beach | South Bay | 73.528 | 118.332 | SR 80 east (Main Street / West Palm Beach Road) to US 441 / SR 715 – Belle Glade, West Palm Beach | Southern end of SR 80 concurrency |
| Hendry | ​ | 87.203 | 140.340 | CR 835 south (Evercane Road) | |
| Hendry | Clewiston | 89.384 | 143.850 | W.C. Owen Avenue (CR 832) | |
| Hendry | ​ | 95.958 | 154.429 | CR 720 west | |
| Hendry | Whidden Corner | 98.377 | 158.322 | SR 80 west – LaBelle, Ft. Myers, Big Cypress Seminole Reservation | Northern end of SR 80 concurrency |
| Glades | ​ | 102.379 | 164.763 | CR 720 east | |
| Glades | ​ | 103.9 | 167.2 | Daniels Road to River Road | Interchange |
| Glades | Moore Haven | 104.2 | 167.7 | 3rd Street to North Frontage Road | Southbound exit and northbound entrance; interchange |
| Glades | ​ | 106.129 | 170.798 | SR 78 east – Lakeport, Seminole Reservation, Okeechobee | Southern end of SR 78 concurrency |
| Glades | ​ | 110.110 | 177.205 | SR 78 west – LaBelle | Northern end of SR 78 concurrency |
| Glades | Harrisburg | 120.599 | 194.085 | SR 29 south – LaBelle, Immokalee | |
| Glades | ​ | 121.677 | 195.820 | CR 733 north (3rd Street) | |
| Glades | Palmdale | 122.055 | 196.428 | Broadway Street (CR 733 south) | |
| Glades | ​ | 127.979 | 205.962 | CR 17 north (Detjens Dairy Road) | |
| Highlands | ​ | 130.749 | 210.420 | CR 731 south – Venus | |
| Highlands | Bairs Den | 140.536 | 226.171 | SR 70 – Arcadia, Okeechobee | |
| Highlands | ​ | 144.825 | 233.073 | CR 29 south | |
| Highlands | ​ | 144.946 | 233.268 | Old State Road 8 | |
| Highlands | Lake Placid | 146.283 | 235.420 | CR 17 north (South Main Avenue) | |
| Highlands | Lake Placid | 147.043 | 236.643 | CR 17A west (Interlake Boulevard) / CR 621 east – Downtown | |
| Highlands | ​ | 148.589 | 239.131 | CR 17 south / CR 621 west (Lake June Road) – Lake June in Winter Scrub State Park | |
| Highlands | ​ | 150.296 | 241.878 | CR 17 north | |
| Highlands | ​ | 157.342 | 253.217 | US 98 south (SR 700) / SR 66 west – Airport, Zolfo Springs, Sebring International Raceway, Okeechobee, Spring Lake | Southern end of US 98/SR 700 concurrency |
| Highlands | Sebring | 160.097 | 257.651 | CR 17A north (Highlands Avenue) – Fairground, Amtrak, truck route to SR 17 | |
| Highlands | Sebring | 160.847 | 258.858 | SR 17 north – Sebring Historic District | |
| Highlands | Sebring | 162.830 | 262.049 | CR 634 west – Highlands Hammock State Park | |
| Highlands | Sebring | 163.596 | 263.282 | CR 634 Alt. west (Flare Road / CR 634 east) | |
| Highlands | Sebring | 165.816 | 266.855 | Sebring Parkway (CR 634A east) - Sebring | |
| Highlands | Avon Park | 171.572 | 276.118 | SR 17 south / SR 64 west (Main Street) – Wauchula, Zolfo Springs, Avon Park Air Force Range, Historical District, Airport | |
| Highlands | Avon Park | 173.074 | 278.536 | CR 17A south / Stryker Road – Avon Park Lakes, truck route to SR 17 / CR 64 | |
| Polk | ​ | 177.968 | 286.412 | SR 17 north (Scenic Highway) / North Avon Park Cut-Off – Frostproof | |
| Polk | ​ | 182.234 | 293.277 | US 98 north (SR 700) / CR 630 east – Ft. Meade, Frostproof, Indian Lake Estates | Northern end of US 98/SR 700 concurrency |
| Polk | ​ | 183.995 | 296.111 | CR 630A east – Frostproof, Indian Lake Estates | |
| Polk | ​ | 190.269 | 306.208 | CR 640 east / Alta / Bab Cut-Off Road – Babson Park, Webber International University | |
| Polk | Lake Wales | 192.372 | 309.593 | CR 17B east (Hunt Brothers Road) | |
| Polk | Lake Wales | 194.20 | 312.53 | SR 60 – Vero Beach, Tampa, Lake Kissimmee State Park | Interchange; access to Lake Wales Medical Center |
| Polk | Lake Wales | 196.100 | 315.592 | Mountain Lake Cut Off Road North - Bok Tower Gardens | |
| Polk | Lake Wales | 198.530 | 319.503 | CR 17A east (Chalet Suzanne Road) / Thompson Nursery Road – Historic Chalet Suzanne | |
| Polk | Winter Haven | 200.143 | 322.099 | SR 540 west (Cypress Gardens Boulevard) / Waverly Road – Legoland, Winter Haven, Waverly | |
| Polk | Dundee | 202.883 | 326.509 | SR 542 west (Dundee Road) – Winter Haven, Dundee | |
| Polk | Lake Hamilton | 205.476 | 330.682 | CR 546 east (Kokomo Road) | |
| Polk | Winter Haven | 207.432 | 333.829 | SR 544 – Winter Haven, Auburndale, Grenelefe | |
| Polk | Haines City | 209.28 | 336.80 | US 17 / US 92 (SR 600) to SR 17 – Haines City, Lake Alfred | Interchange |
| Polk | Haines City | 210.327 | 338.488 | CR 17 (Old Polk City Road / Main Street) – Business District | |
| Polk | Haines City | 212.866 | 342.575 | CR 547 east / Sanders Road – Davenport | |
| Polk | Four Corners | 218.15 | 351.08 | I-4 (SR 400) – Orlando, Tampa, St. Petersburg | I-4 exit 55 |
| Polk | Four Corners | 219.750 | 353.653 | Deen Still Road / Ronald Reagan Parkway (CR 54 east) - Loughman | |
| Lake | Four Corners | 226.19 | 364.02 | US 192 east (SR 530) – Disney World, Kissimmee | Interchange |
| Lake | Four Corners | 227.892 | 366.757 | CR 474 west | |
| Lake | ​ | 232.477 | 374.135 | SR 516 east (Lake-Orange Expressway) | |
| Lake | Clermont | 240.96 | 387.79 | SR 50 – Clermont, Orlando | Interchange; access to South Lake Hospital |
| Lake | Minneola | 242.972 | 391.026 | CR Old 50 east (Washington Street) | |
| Lake | Minneola | 243.536 | 391.933 | Main Avenue | Former CR 561 south |
| Lake | Minneola | 244.029 | 392.727 | CR 561 south (Lake Minneola Shores) | Southern end of CR 561 concurrency; former CR 561A south |
| Lake | ​ | 246.165 | 396.164 | CR 561 north – Astatula | Northern end of CR 561 concurrency |
| Lake | Groveland | 249.52 | 401.56 | SR 19 – Howey-in-the-Hills, Tavares, Groveland | Interchange |
| Lake | Groveland | 249.689 | 401.835 | Florida's Turnpike south (SR 91) – Miami | Turnpike exit 285 |
| Lake | ​ | 252.209 | 405.891 | CR 565 south | |
| Lake | ​ | 253.551 | 408.051 | Florida's Turnpike north (SR 91) – Ocala | Turnpike exit 289 |
| Lake | Leesburg | 258.824 | 416.537 | CR 48 to Florida's Turnpike north – Okahumpka, Howey-in-the-Hills | |
| Lake | ​ | 259.879 | 418.235 | CR 33 south – Mascotte, Dade Battlefield Historic State Park | |
| Lake | Leesburg | 260.907 | 419.889 | CR 25A north (Connell Road) – Flat Island Preserve | |
| Lake | Leesburg | 262.107 | 421.820 | CR 25A south – Flat Island Preserve | |
| Lake | Leesburg | 262.741 | 422.841 | SR 44 (Dixie Avenue / South Street) to I-75 / US 441 south – Wildwood, Eustis | |
| Lake | Leesburg | 264.329 | 425.396 | US 441 south (SR 500) – Orlando | south end of US 441/SR 500 concurrency |
| see US 441 (mile 289.300-320.451) ||||||
| Marion | Ocala | 295.480 | 475.529 | US 301 north / US 441 north (North Pine Avenue / SR 200 / SR 500) / SR 492 east (Bonnie Heath Boulevard) to SR 40 – Gainesville, Silver Springs | Northern end of US 301/US 441/SR 200/SR 500 concurrency |
| Marion | Ocala | 298.29 | 480.05 | I-75 (SR 93) – Lake City, Tampa | I-75 exit 354 |
| Marion | ​ | 301.680 | 485.507 | CR 225A | |
| Marion | ​ | 304.972 | 490.805 | CR 225 north | |
| Marion | Fellowship | 305.476 | 491.616 | CR 464B west | |
| Marion | Blitchton | 309.361 | 497.868 | CR 326 – Morriston, Zuber | |
| Marion | ​ | 316.027 | 508.596 | CR 316 east | |
| Levy | ​ | 316.203 | 508.879 | CR 335 north | |
| Levy | ​ | 317.248 | 510.561 | CR 316 (Northeast 30th Street) | |
| Levy | ​ | 318.555 | 512.665 | CR 318 east | |
| Levy | Williston | 318.965 | 513.324 | CR 323 south (Southeast 8th Street) | |
| Levy | Williston | 319.602 | 514.350 | US 27 Alt. north / US 41 south / SR 121 south (Noble Avenue / SR 45 south / SR 500 north) – Dunnellon, Tallahassee | Northern end of SR 500 concurrency; southern end of US 41/SR 121/SR 45 concurrency |
| see US 41 (mile 375.158-408.641) ||||||
| Alachua | High Springs | 353.085 | 568.235 | SR 20 south (Northeast 1st Avenue) / US 41 north (North Main Street / SR 45) – Alachua, Lake City | Northern end of US 41/SR 45 concurrency; southern end of SR 20 concurrency |
| Santa Fe River | Santa Fe River | | | Bridge over the Santa Fe River | Bridge over the Santa Fe River |
| Columbia | ​ | 356.377 | 573.533 | CR 138 west | |
| Columbia | ​ | 359.182 | 578.047 | CR 778 east | |
| Columbia | Fort White | 362.729 | 583.756 | CR 18 east – Worthington Springs | |
| Columbia | Fort White | 362.999 | 584.190 | SR 47 (1st Street) – Trenton, Ichetucknee Springs, Lake City | |
| Ichetucknee River | Ichetucknee River | | | Bridge over the Ichetucknee River | Bridge over the Ichetucknee River |
| Suwannee | Hildreth | 369.456 | 594.582 | CR 137 north | |
| Suwannee | ​ | 372.470 | 599.432 | US 129 south (SR 49) / CR 49 north – Bell, Live Oak | Southern end of US 129 concurrency |
| Suwannee | Branford | 376.544 | 605.989 | US 129 north (Southwest Suwannee Avenue / SR 249) – Live Oak, Jasper | Northern end of US 129 concurrency |
| Suwannee River | Suwannee River | | | Frank R. Norris Bridge | Frank R. Norris Bridge |
| Lafayette | ​ | 377.889 | 608.153 | SR 349 south – Old Town | |
| Lafayette | ​ | 380.720 | 612.709 | CR 405 | Former SR 354 |
| Lafayette | ​ | 384.766 | 619.221 | CR 416 | Former SR 354A |
| Lafayette | ​ | 385.435 | 620.298 | CR 411 | Former SR 251 |
| Lafayette | ​ | 385.561 | 620.500 | CR 412 | Former SR 251 |
| Lafayette | ​ | 386.785 | 622.470 | CR 412 | Former SR 251 |
| Lafayette | ​ | 389.321 | 626.551 | CR 354 west / CR 405 – Convict Spring | Former SR 354 |
| Lafayette | Mayo | 394.061 | 634.180 | SR 51 (Fletcher Avenue) – Steinhatchee, Live Oak | |
| Lafayette | Mayo | 394.382 | 634.696 | CR 300 | Former SR 251A |
| Lafayette | ​ | 395.457 | 636.426 | CR 320 | Former SR 251 |
| Lafayette | ​ | 397.779 | 640.163 | CR 534 / CR 536 | Former SR 53 |
| Lafayette | ​ | 398.874 | 641.925 | CR 292 – Lafayette Blue Springs State Park | Former SR 251B |
| Lafayette | ​ | 400.632 | 644.755 | CR 251 north / CR 350 south | Former SR 251/SR 350 |
| Lafayette | Buckville | 403.058 | 648.659 | CR 53 north / CR 534 – Day, Dowling Park, Madison | Former SR 53 |
| Lafayette | Townsend | 405.873 | 653.189 | CR 348 east | Former SR 348 |
| Taylor | ​ | 416.660 | 670.549 | Fenholloway Road | Former SR 356 |
| Taylor | ​ | 416.864 | 670.878 | San Pedro Road | Former SR 356 |
| Taylor | ​ | 417.613 | 672.083 | CR 30 west (Foley Road) – Foley | |
| Taylor | ​ | 418.838 | 674.054 | Foley Cut Off | Former SR 356C |
| Taylor | ​ | 420.331 | 676.457 | Buckeye Nursery Road | Former SR 361 north |
| Taylor | ​ | 420.457 | 676.660 | Plantation Road | Former SR 361 south |
| Taylor | Perry | 421.925 | 679.022 | US 221 (Jefferson Street / SR 55) | |
| Taylor | Perry | 422.350 | 679.706 | US 19 south / US 27 Alt. south / US 98 / US 221 Truck south (Byron Butler Parkway / Hampton Springs Avenue / SR 30) – Cross City, St. Petersburg | Southern end of US 19/US 221 Truck concurrency |
| Taylor | Perry | 423.309 | 681.250 | CR 356 (West Julia Street) | Former SR 356 west |
| Taylor | Perry | 423.615 | 681.742 | West Ash Street | Former SR 356 east |
| Taylor | ​ | 424.399 | 683.004 | Andrew Reams Road | Former SR 356B; southbound access only |
| Taylor | ​ | 425.144 | 684.203 | US 221 Truck north / CR 359A (Wright Road / Slaughter Road) | Northern end of US 221 Truck concurrency |
| Taylor | ​ | 426.672 | 686.662 | CR 361 (Pisgah Road / Harrison Blue Road) | |
| Taylor | Eridu | 438.588 | 705.839 | CR 14 east (Alton Wentworth Road) | |
| Madison | ​ | 440.159 | 708.367 | CR 150 east (Southwest Overstreet Avenue) | |
| Aucilla River | Aucilla River | | | Bridge over the Aucilla River | Bridge over the Aucilla River |
| Jefferson | Lamont | 445.162 | 716.419 | CR 257A south (South Salt Road) | |
| Jefferson | Lamont | 445.244 | 716.551 | CR 257B north to I-10 – Aucilla | |
| Jefferson | Capps | 451.558 | 726.712 | US 19 north (Florida Georgia Parkway / SR 57) to I-10 – Monticello | Northern end of US 19 concurrency |
| Jefferson | Waukeenah | 454.072 | 730.758 | CR 259 (Waukeenah Highway) – Wacissa | |
| Jefferson | ​ | 458.141 | 737.306 | SR 59 to I-10 – Wacissa, Lloyd | |
| Leon | Chaires Crossroads | 464.329 | 747.265 | CR 154 (Chaires Cross Road) / CR 2195 (W.W. Kelley Road) – Chaires, Capitola, Wakulla Correctional Institution | |
| Leon | ​ | 466.679 | 751.047 | CR 2197 south (Williams Road) | |
| Leon | Tallahassee | 470.916 | 757.866 | US 319 (Capital Circle Southeast / SR 261) – National Guard Armory, Federal Correctional Institution, Tallahassee International Airport | |
| Leon | Tallahassee | 472.342 | 760.161 | CR 373 south (Blair Stone Road) | |
| Leon | Tallahassee | 472.86 | 760.99 | Old St. Augustine Road (CR 2196) | Eastbound access only |
| Leon | Tallahassee | 473.144 | 761.451 | SR 265 north / CR 265 south (Magnolia Drive) | |
| Leon | Tallahassee | 474.1 | 763.0 | Franklin Boulevard (CR 1555), Thomasville Road (SR 61 north) via Gadsden Street (CR 1557 north) | Northbound exit and southbound entrance; interchange |
| Leon | Tallahassee | 474.222 | 763.186 | CR 1559 south (Calhoun Street) | |
| Leon | Tallahassee | 474.287 | 763.291 | SR 61 south (Monroe Street) | Southern end of SR 61 concurrency |
| Leon | Tallahassee | 474.388 | 763.453 | SR 366 west (Jefferson Street) | |
| Leon | Tallahassee | 474.740 | 764.020 | US 90 (Tennessee Street / SR 10 / SR 20 west) – Florida State University, Quincy, Monticello | Northern end of SR 20 concurrency |
| Leon | Tallahassee | 475.126 | 764.641 | SR 61 north (Thomasville Road) – Thomasville | Northern end of SR 61 concurrency; southern end of SR 63; no left turn southbound |
| Leon | Tallahassee | 476.073 | 766.165 | CR 158 west (Tharpe Street) | |
| Leon | Tallahassee | 478.17 | 769.54 | I-10 (SR 8) – Lake City, Pensacola | I-10 exit 199 |
| Leon | Tallahassee | 480.070 | 772.598 | CR 0356 west (Fred George Road) / Crowder Road – Lake Jackson Mounds Archaeological State Park | |
| Leon | Lake Jackson | 482.951 | 777.234 | SR 263 south / CR 0361 (Capital Circle Northwest / Old Bainbridge Road) – Tallahassee International Airport | |
| Ochlockonee River | Ochlockonee River | | | Bridge over the Ochlockonee River | Bridge over the Ochlockonee River |
| Gadsden | ​ | 486.065 | 782.246 | CR 270 west (Shady Rest Road) | |
| Gadsden | ​ | 488.750 | 786.567 | CR 159 south (Scotland Road) | |
| Gadsden | Havana | 489.948 | 788.495 | SR 159 north – Quincy | |
| Gadsden | Havana | 490.787 | 789.845 | SR 12 west / CR 12A east (9th Avenue) – Quincy | |
| Gadsden | Havana | 491.039 | 790.251 | CR 12 east (5th Avenue East) | |
| Gadsden | Hinson | 492.840 | 793.149 | CR 159A south (Potter Woodbery Road) | |
| Gadsden | ​ | 493.391 | 794.036 | CR 12B east (Glade Road) | |
| Gadsden | Darsey | 496.216 | 798.582 | CR 157 south (Concord-Bainbridge Road) to SR 111 / Darsey-Calvary Road – Calvary, GA | |
| Gadsden | ​ | 496.352 | 798.801 | US 27 north / SR 1 north – Attapulgus, Bainbridge | Georgia state line |
| 1.000 mi = 1.609 km; 1.000 km = 0.621 mi Concurrency terminus Incomplete access Unopened ||||||

## Related routes

At least five special routes of US 27 have existed in Florida, although only one exists today:
* U.S. Highway 27 Alternate – Sebring to Avon Park; former route
* U.S. Highway 27 Temporary – Sebring to Leesburg; former route
* U.S. Highway 27 Alternate – Sunray Deli Estates (north of Avon Park) to Haines City; former route
* U.S. Highway 27 Alternate – Lady Lake to Belleview; former route cosigned with US 441 Alt.
* U.S. Highway 27 Alternate – Williston to Perry