# List of fictional non-binary characters

This is a list of fictional non-binary characters (i.e. genderqueer) identified as such in anime, animation, print media, feature films, live-action television, theatre, video games, webcomics, and other mediums.
Queer identifications listed include characters of non-binary gender, agender, bigender, genderfluid, genderqueer, as well as characters of any third gender.
For more information about fictional characters in other identifications of the LGBTQ community, see the lists of lesbian, bisexual, gay, transgender, aromantic, asexual, intersex, and pansexual characters.
The names are organized alphabetically by surname (i.e. last name), or by single name if the character does not have a surname. If more than two characters are in one entry, the last name of the first character is used.

## Anime and animation

| Character | Show title | Portrayed by | Identity | Duration | Notes |
| Tsugumi Aozora | Asobi Asobase | Aoi Yūki | Genderqueer | 2018 | Tsugumi is a classmate who the Pastimers suspect of being an otokonoko. |
| Yū Asuka | Stars Align | Yoshitaka Yamaya | Non-binary | 2019 | Yū, formerly known as Yuta, is a kind and mild-mannered person, who Touma thinks of them as nice, even though he is unaware Yū has a crush on him, as noted in the second episode. In one episode, Yū revealed that they wear women's clothing, not sure of whether they are "binary trans, x-gender, or something else entirely" and is still figuring their gender identity. |
| Yū Asuka | Stars Align | Yoshitaka Yamaya | Gender non-conforming | 2019 | Yū, formerly known as Yuta, is a kind and mild-mannered person, who Touma thinks of them as nice, even though he is unaware Yū has a crush on him, as noted in the second episode. In one episode, Yū revealed that they wear women's clothing, not sure of whether they are "binary trans, x-gender, or something else entirely" and is still figuring their gender identity. |
| Asher Berdacs | Kipo and the Age of Wonderbeasts | River Butcher | Non-binary | 2020 | When asked by a fan about the gender of Asher, series creator Radford "Rad" Sechrist said that Asher is non-binary and uses singular they pronouns, which was later confirmed by Bill Wolkoff, co-screenwriter of Kipo. |
| Blaine | Adventure Time: Distant Lands; Adventure Time: Fionna and Cake (cameo) | Bex Taylor-Klaus | Non-binary | 2023 | Blaine is a young cyclops and a classmate of the character Peppermint Butler. Blaine is referred to with the singular they pronoun in "Distant Lands". Moreover, the official Adventure Time Tumblr account posted concept art of Blaine, including notes referring to them with such language. |
| BMO | Adventure Time | Niki Yang | Genderfluid | 2010–2023 | BMO is a fun-loving, childlike, sentient game console. BMO is also genderfluid, with characters using masculine and feminine pronouns interchangeably to refer to him. Other robotic characters in the show have binary genders, such as NEPTR, a robot built to throw pies at people who is consistently recognized as male. |
| Courtney Cahatel | Dead End: Paranormal Park | Emily Osment | Non-binary | 2022 | Series creator Hamish Steele said that Courtney is not "aware of gender in any way" and cannot be misgendered, noting that all pronouns were used in production. He added that he uses they/them for Courtney, but that Netflix persuaded them to use the pronouns of the person cast for Courtney's voice role. Emily Osment) was cast, so Courtney is referred to with the pronouns "she" and "her". |
| Double Trouble | She-Ra and the Princesses of Power | Jacob Tobia | Non-binary | 2018–2020 | Showrunner ND Stevenson described them at New York Comic Con 2019 as a "nonbinary shapeshifting mercenary". They are voiced by Jacob Tobia, a non-binary person. Double Trouble reappears for brief periods in the show's final season, posing as "Peekablue," a male prince. |
| Odee Elliott | Madagascar: A Little Wild | iris menas | Non-binary | 2021–2022 | An okapi, voiced by iris menas, who first appears in the season 3 Pride-themed episode "Whatever Floats Your Float," with none of the floats seeming right for Odee, and then sings a song titled "Be Proud" about being proud of your identity. GLAAD consulted on the episode and menas said the episode resonated with hir. |
| François | Dr. Stone | Maaya Sakamoto | GenderFluid | 2019–present | François is Ryusui's faithful butler whose services were well sought out and coveted even before the Petrifucation. |
| Fred | Ridley Jones | iris menas | Non-binary | 2021–2023 | Fred is a non-binary bison who prefers they/them pronouns and is the first non-binary character in a Netflix kids series. |
| Garnet | Steven Universe | Estelle | Non-binary | 2013–2020 | Ruby and Sapphire are two sexless but feminine-presenting members of the Crystal Gems who have a romantic relationship with each other, and stay permanently fused to form Garnet. In July 2015, the co-executive producer, Ian Jones-Quartey, has confirmed that, according to human standards and terminology, calling Ruby and Sapphire non-binary, feminine-presenting lesbians would be "a fair assessment". On July 6, 2018, the episode "Reunited" aired, in which Ruby and Sapphire get married, kiss, and fuse into Garnet, after Ruby proposed to Sapphire in a previous episode, "The Question". Series creator Rebecca Sugar has also said that "the Gems are all non-binary women," which includes Garnet, and her friends, Amethyst and Pearl. |
| Garnet | Steven Universe Future | Estelle | Non-binary | 2013–2020 | Ruby and Sapphire are two sexless but feminine-presenting members of the Crystal Gems who have a romantic relationship with each other, and stay permanently fused to form Garnet. In July 2015, the co-executive producer, Ian Jones-Quartey, has confirmed that, according to human standards and terminology, calling Ruby and Sapphire non-binary, feminine-presenting lesbians would be "a fair assessment". On July 6, 2018, the episode "Reunited" aired, in which Ruby and Sapphire get married, kiss, and fuse into Garnet, after Ruby proposed to Sapphire in a previous episode, "The Question". Series creator Rebecca Sugar has also said that "the Gems are all non-binary women," which includes Garnet, and her friends, Amethyst and Pearl. |
| Zoë Hange | Attack on Titan | Romi Park (Japanese) | Ambiguous | 2013–2023 | Hange Zoë is a Section Commander of the Scouting Regiment who serves as its veteran leader of 4th Squad and a scientist who studies the Titans. In the original English translation of the original manga, Hange is referred to as a female, and is also portrayed as one in the anime adaptation. However, in a blog post in 2011, Isayama responded to a question regarding Hange's gender, saying, "Perhaps [Hange's gender] is better left unstated". In 2014, Kodansha USA stated they went back through volume 5 and removed gender-specific pronouns they had used for reprint, and references from volume 6 onwards. |
| Zoë Hange | Attack on Titan | Jessica Calvello (English) | Ambiguous | 2013–2023 | Hange Zoë is a Section Commander of the Scouting Regiment who serves as its veteran leader of 4th Squad and a scientist who studies the Titans. In the original English translation of the original manga, Hange is referred to as a female, and is also portrayed as one in the anime adaptation. However, in a blog post in 2011, Isayama responded to a question regarding Hange's gender, saying, "Perhaps [Hange's gender] is better left unstated". In 2014, Kodansha USA stated they went back through volume 5 and removed gender-specific pronouns they had used for reprint, and references from volume 6 onwards. |
| Violet Harper (Halo) | Young Justice | Zehra Fazal | Non-binary | 2010–2022 | Violet Harper, also known as Halo, is the soul of a sentient technology known as a Mother Box that entered the body of Gabrielle Dhaou. In spite of Halo's outward appearance, the character does not identify as male or female as defined in Earth language.". In the episode "Nautical Twilight", Halo self-identifies as non-binary, adding that the singular they pronoun suits them best, although they are unbothered by being called "she". Rocket and the New Gods used feminine pronouns for Halo in the episode "Forbidden Secrets of Civilizations Past!" though this was a writing mistake according to Greg Weisman. |
| Winn Harper | The Fairly OddParents: A New Wish | iris menas | Non-binary | 2024 | A cool, purple-haired elementary schooler fond of skateboarding. They're referred to with singular they pronouns in the show. |
| Jae | Supernatural Academy | Ali J. Eisner | Non-binary | 2022 | Jae is a Faerie student who goes to Supernatural Academy. They are non-binary and prefer they/them pronouns. Jae is voiced by transgender and non-binary actor Ali J. Eisner. |
| Angel Jose | Craig of the Creek | Angel Lorenzana | Agender | 2018–present | One of the characters, Angel, is non-binary and uses they/them pronouns. They are voiced by Angel Lorenzana who is a storyboard artist and writer for the show, who identifies as agender and uses the same pronouns. |
| Supreme Kai | Dragon Ball Daima | Nia Celeste (English) | Genderless | 2024 | In Dragon Ball DAIMA, it is revealed that the Kais, specifically the Supreme Kai, are part of the Glind race, which is genderless. |
| Supreme Kai | Dragon Ball Daima | Yumiko Kobayashi (Japanese) | Genderless | 2024 | In Dragon Ball DAIMA, it is revealed that the Kais, specifically the Supreme Kai, are part of the Glind race, which is genderless. |
| Brother Ken | bro'Town | David Fane | Fa'afafine | 2004–2009 | Brother Ken is the principal of the school and is fa'afafine, a person in Samoa, American Samoa and the Samoan diaspora who identifies themselves as having a third gender or non-binary role, and is an integral part of Samoan culture. Those who are fa'afafine have behaviour which ranges from extravagantly feminine to conventionally masculine. Because the concept does not readily translate, when the series was broadcast on Adult Swim Latin America, a decision was made not to translate Samoan words and just present them as part of the "cultural journey". |
| Kino | Kino's Journey | Ai Maeda | Transmasculine nonbinary | 2003 | Kino is a traveler who goes to "unknown mystical places" with a talking motorcycle named Hermes. Kino was assigned female at birth, but has a "androgynous persona," alternating between using feminine and masculine pronouns, while resisting those that attempt to pin a gender on them as a "girl" or "boy." While the show's official website, described Kino as a "young man," the series creator, Keiichi Sigsawa, said in September 2017, "Kino really doesn't think of herself too deeply when she uses pronouns. Depending on the circumstances, she may use "boku" or "atashi", it kinda depends on the situation she finds herself in," adding that it was not he "wanted to write a story about a girl who lives like a boy directly, [but] it was just an interesting way to start the story, and her character's personality has continued that way since then." As such, some reviewers even described Kino as one of the "rare transmasculine anime protagonists." |
| Milo | Danger & Eggs | Tyler Ford | Agender | 2017 | In the fifth episode, the two protagonists, DD Danger and Phillip, meet Milo, who uses they/them pronouns. In the following episode, they form a band with DD and Philip named the Buck Buck Trio and play a music festival together. Tyler Ford, an agender model and speaker is the voice of Milo, said they loved that their character, is an "accurate representation" of them. |
| Morph | X-Men '97 | Rob Rubin, J. P. Karliak | Nonbinary | (1992-1997), (2024–present) | Introduced in X-Men: The Animated Series, Morph is a mutant member of the X-men with the power of shapeshifting. In X-Men '97, Morph is referred to with both they/them and he/him pronouns. |
| Neferpitou | Hunter x Hunter | Ayumi Fujimura | Ambiguous | 2011–2014 | The official English translation opts for gender-neutral pronouns. The only official statement regarding Neferpitou's gender appears in the Hunter × Hunter Hunter Association Official Issue: Hunter's Guide; Character & World Official Databook. It refers to Neferpitou using the pronoun 彼 (kare), which is usually translated to “he”. |
| Obsidian | Steven Universe | Various actors overlayed | Non-binary | 2013–2019 | A fusion of Steven Universe and fellow Crystal Gems Garnet, Amethyst and Pearl, Obsidian is based on the volcanic glass obsidian. Obsidian first appeared in the episode "Change Your Mind." They later were imagined in the episode "In Dreams," and shown in a flashback in "Growing Pains." Joe Johnson, a storyboard artist for the show confirmed that Obsidian uses they/them and she/her pronouns. |
| Najimi Osana | Komi Can't Communicate | Rie Murakawa | Ambiguous | 2021–2022 | Described as having an ambiguous/unknown gender/sex by official Japanese website. Najimi is a friend of the main characters, and has a habit of switching their gender, wearing a school uniform with a skirt, but a boy's tie. |
| Nightshade | Transformers: EarthSpark | Z Infante | Non-binary | 2022–present | One of the new members of Terrans, Transformers born on Earth and ally of the Autobots and the Malto family who first appears in the two-part episode "Age of Evolution". Nightshade explains to Optimus Prime that they go by they/them pronouns, as "he or she just doesn't fit who [they are]". |
| Rainbow Quartz 2.0 | Steven Universe | Alastair James | Non-binary | 2013–2020 | A fusion of Steven Universe and fellow Crystal Gem Pearl, Rainbow Quartz 2.0 is based on the metal-coated crystal rainbow quartz. He debuts in "Change Your Mind" and reappears in "A Very Special Episode." Rainbow Quartz 2.0 uses both they/them and he/him pronouns, the only fusion to use these pronouns together, as confirmed by Colin Howard, a character designer, former writer and storyboard artist for Steven Universe and Steven Universe Future. |
| Rainbow Quartz 2.0 | Steven Universe Future | Alastair James | Non-binary | 2013–2020 | A fusion of Steven Universe and fellow Crystal Gem Pearl, Rainbow Quartz 2.0 is based on the metal-coated crystal rainbow quartz. He debuts in "Change Your Mind" and reappears in "A Very Special Episode." Rainbow Quartz 2.0 uses both they/them and he/him pronouns, the only fusion to use these pronouns together, as confirmed by Colin Howard, a character designer, former writer and storyboard artist for Steven Universe and Steven Universe Future. |
| River | Rubble & Crew | Chinag Ma | Non-binary | 2023—present | River is a non-binary skateboarder. River uses they/them pronouns. River is voiced by non-binary actor Chinag Ma. |
| Val/entina Romanyszyn | Gen:Lock | Asia Kate Dillon | Genderfluid | 2019–2021 | In the episode "Training Daze", Val(entina) mentioned that they are genderfluid, going by the name "Val" when male-presenting and "Valentina" when female-presenting. In the episode "Together. Together," Val is revealed to be pansexual. Austin Chronicle reported that the character was written as genderfluid, but is feminine-presenting, altering their gender performance several times. |
| Sailor Star Fighter (Kou Seiya) | Sailor Moon | Shiho Niiyama | Genderqueer | 1992–1997 | In the 90s anime, the Sailor Starlights (Sailor Star Fighter, Sailor Star Maker, and Sailor Star Healer) were assigned female at birth, but transform to present as male and refer to themselves as males when not fighting, as shown in the episode, "Holy War in the Galaxy! Sailor Wars Legend." In a 1999 interview, Takeuchi said she had made them always girls in the manga, in the anime "they got turned into guys," which displeased her and I wasn't very happy about it. |
| Sailor Star Healer (Kou Yaten) | Sailor Moon | Chika Sakamoto | Genderqueer | 1992–1997 | In the 90s anime, the Sailor Starlights (Sailor Star Fighter, Sailor Star Maker, and Sailor Star Healer) were assigned female at birth, but transform to present as male and refer to themselves as males when not fighting, as shown in the episode, "Holy War in the Galaxy! Sailor Wars Legend." In a 1999 interview, Takeuchi said she had made them always girls in the manga, in the anime "they got turned into guys," which displeased her and I wasn't very happy about it. |
| Sailor Star Maker (Kou Taiki) | Sailor Moon | Narumi Tsunoda | Genderqueer | 1992–1997 | In the 90s anime, the Sailor Starlights (Sailor Star Fighter, Sailor Star Maker, and Sailor Star Healer) were assigned female at birth, but transform to present as male and refer to themselves as males when not fighting, as shown in the episode, "Holy War in the Galaxy! Sailor Wars Legend." In a 1999 interview, Takeuchi said she had made them always girls in the manga, in the anime "they got turned into guys," which displeased her and I wasn't very happy about it. |
| Sailor Uranus (Haruka Tenoh) | Sailor Moon Crystal | Junko Minagawa | Non-binary | 2014–2016 | Haruka is referred to by her partner, Sailor Neptune, as "both male and female, but also neither", making Haruka the first character in the franchise to be referred to that way. She is also said to have an independent attitude and not care much for teamwork. She also reappears in the film, Sailor Moon Eternal, as does Neptune. |
| Princess Sapphire | Princess Knight | Toma Yumi | Ambiguous | 1967–1968 | Princess Sapphire is raised as a boy by her father since women are not eligible to inherit the throne. In addition, she is born with both a male and female heart but refuses to give up her boy heart as she needs it to vanquish evil. Nonetheless, she falls in love with and marries Prince Frank. |
| Nathan Seymour (Fire Emblem) | Tiger & Bunny | Kenjiro Tsuda (Japanese) | Gay | 2011 | Nathan is a highly effeminate homosexual man who identifies as genderqueer though he prefers to be identified as a woman at times, often spending more time with the female heroes while flirting with the male heroes. In the past, they tried to present themselves femininely but was harshly criticized, and they still hold a strong romantic infatuation towards men. |
| Nathan Seymour (Fire Emblem) | Tiger & Bunny | John Eric Bentley (English) | Genderqueer | 2011 | Nathan is a highly effeminate homosexual man who identifies as genderqueer though he prefers to be identified as a woman at times, often spending more time with the female heroes while flirting with the male heroes. In the past, they tried to present themselves femininely but was harshly criticized, and they still hold a strong romantic infatuation towards men. |
| Shep | Steven Universe Future | Indya Moore | Non-binary | 2020 | Partner of Sadie Miller, voiced by Indya Moore who is also non-binary, transgender, uses gender neutral they/them pronouns, and is a person of color. In their episode debut in "Little Graduation," Shep helped Steven work out his mental problems and come to his senses. |
| Smoky Quartz | Steven Universe | Natasha Lyonne | Non-binary | 2013–2020 | A fusion of Steven Universe, and fellow Crystal Gem, Amethyst, Smoky is based on the crystal smoky quartz. Steven and Amethyst first fused at a low point for both characters as noted by Michaela Dietz, the voice actress for Amethyst on the official Steven Universe podcast. Smoky debuted in the episode "Earthlings," and reappeared in three other episodes: "Know Your Fusion," "Change Your Mind", and "Guidance." Rebecca Sugar has stated that the Gems are "all non-binary women," with this applying to Amethyst specifically. On November 10, 2017, Cartoon Network posted a YouTube video of the character Peridot playing Steven Universe: Save the Light, in which she refers to Smoky with the singular they pronoun. |
| Smoky Quartz | Steven Universe Future | Natasha Lyonne | Non-binary | 2013–2020 | A fusion of Steven Universe, and fellow Crystal Gem, Amethyst, Smoky is based on the crystal smoky quartz. Steven and Amethyst first fused at a low point for both characters as noted by Michaela Dietz, the voice actress for Amethyst on the official Steven Universe podcast. Smoky debuted in the episode "Earthlings," and reappeared in three other episodes: "Know Your Fusion," "Change Your Mind", and "Guidance." Rebecca Sugar has stated that the Gems are "all non-binary women," with this applying to Amethyst specifically. On November 10, 2017, Cartoon Network posted a YouTube video of the character Peridot playing Steven Universe: Save the Light, in which she refers to Smoky with the singular they pronoun. |
| Izana Shinatose | Knights of Sidonia | Aki Toyosaki | Third gender | 2014–2015 | Izana belongs to a new, nonbinary third gender that originated during the hundreds of years of human emigration into space, as first shown in the episode "Commencement." Izana later turns into a girl after falling in love with Nagate Tanasake. |
| Frankie Stein | Monster High | iris menas | Non-binary | 2022 | Frankie Stein is a monster who was created by scientists. Unlike the previous incarnations where Frankie was a cisgender woman, Frankenstein's monster and his bride's daughter, Frankie is a non-binary monster. In the series, and live-action movie, Frankie is a non-binary monster who prefers they/them pronouns. iris menas, who voices Stein, is a non-binary actor. |
| Stevonnie | Steven Universe | AJ Michalka | Non-binary | 2013–2020 | Stevonnie is a fusion of both Steven and Connie. Steven and Connie identify as male and female respectively, but the gender of Stevonnie is difficult to describe, with series creator Rebecca Sugar describing it as the "living relationship between Steven and Connie." Stevonnie is commonly referred to with gender neutral pronouns (such as the singular they), while male and female characters seem to be physically attracted to Stevonnie. |
| Stevonnie | Steven Universe Future | AJ Michalka | Non-binary | 2013–2020 | Stevonnie is a fusion of both Steven and Connie. Steven and Connie identify as male and female respectively, but the gender of Stevonnie is difficult to describe, with series creator Rebecca Sugar describing it as the "living relationship between Steven and Connie." Stevonnie is commonly referred to with gender neutral pronouns (such as the singular they), while male and female characters seem to be physically attracted to Stevonnie. |
| Sunstone | Steven Universe | Shoniqua Shandai | Non-binary | 2013–2020 | A fusion of Steven Universe and fellow Crystal Gem Garnet, Sunstone is based on the mineral sunstone. Debuting in "Change Your Mind" and reappearing in "A Very Special Episode", Sunstone uses both singular they and feminine pronouns as confirmed on the official Steven Universe podcast, with Sunstone's pronouns also confirmed in this episode. |
| Sunstone | Steven Universe Future | Shoniqua Shandai | Non-binary | 2013–2020 | A fusion of Steven Universe and fellow Crystal Gem Garnet, Sunstone is based on the mineral sunstone. Debuting in "Change Your Mind" and reappearing in "A Very Special Episode", Sunstone uses both singular they and feminine pronouns as confirmed on the official Steven Universe podcast, with Sunstone's pronouns also confirmed in this episode. |
| Tai | Moon Girl and Devil Dinosaur | Ian Alexander | Non-binary | 2023–present | Tai is one of Lunella's classmates. Tai is revealed to be non-binary in the episode "Check Yourself" as Tai is referred to with they/them pronouns by Lunella. Tai is voiced by non-binary actor Ian Alexander. |
| Terri | Amphibia | Kate Micucci | Non-binary | 2019–2022 | Terri is a scientist who helped Anne Boonchuy and the Plantars. In the show, they are feminine-presenting and was referred to as a woman by Anne. In the 2022 book Marcy's Journal: A Guide to Amphibia, Marcy uses gender-neutral pronouns for them. |
| Thomas | City of Ghosts | Blue Chapman | Non-binary | 2021 | Thomas is a 7-year-old child who goes by they/them pronouns. They are voiced by transgender child actor Blue Chapman. |
| Raine Whispers | The Owl House | Avi Roque | Non-binary | 2021–2023 | The head witch of the Bard Coven who uses they/them pronouns. Raine is Disney TVA's first non-binary character. The episode "Knock, Knock, Knockin' on Hooty's Door", reveals that Eda and Raine were formerly dating, before breaking up. |
| Raine Whispers | The Owl House | Blu del Barrio (young) | Non-binary | 2021–2023 | The head witch of the Bard Coven who uses they/them pronouns. Raine is Disney TVA's first non-binary character. The episode "Knock, Knock, Knockin' on Hooty's Door", reveals that Eda and Raine were formerly dating, before breaking up. |
| Wren | Middle School Moguls | Tim Gunn | Non-binary | 2019 | One of the professors in the show, Mogul Wren, has been stated to be non-binary. They have a big role in the episode "Mo'gul Money, Mo Problems". |
| Wren | Pinecone & Pony | Ser Anzoategui | Non-binary | 2022–present | Wren is a non-binary person who prefers they/them pronouns, and is in a relationship with Gladys, the teacher for the Lil Rumblers. Wren is voiced by non-binary actor Ser Anzoategui. |
| Shion Zaiden | RWBY: Ice Queendom | Hiroki Nanami | Non-binary | 2022 | A nightmare hunter who captures Grimm who possess people in their dreams. Their presence in the series was praised by some reviewers. While the official Japanese website, refers to Shion with male pronouns, Rooster Teeth treats Shion as nonbinary. Christine Brent, Senior Brand Director for Rooster Teeth, described Shion as fantastic, "totally nonbinary", and unique to this series, while using they/them pronouns for Shion, and said she would like to have similar characters in future productions. |
| Zoit | Lloyd in Space | Pamela Adlon | Agender | 2002 | Zoit is a Padillikon, whose species is neither boy or girl until their 13th birthday, and appears in the episode "Neither Boy Nor Girl," declaring it no one's business what gender they are. |
| 3 and 4 | 9 | | Non-binary | 2009 | Characters 3 and 4 are canonically non-binary. This characterization has been publicly confirmed by director Shane Acker and co-developed with Benjamin Gluck, the head of story. |

## Books, print comics, and manga

| Character | Title | Author | Identity | Year | Notes |
| Aim/the Messenger | Nona the Ninth | Tamsyn Muir | Ambiguous/nonbinary | 2022 | Aim uses they/them pronouns that were "bestowed upon them" by their role as the Messenger (whether these pronouns are singular or plural is ambiguous), is referred to with she/her pronouns and as "sir" by multiple characters, and has been stated by Tamsyn Muir to "live with bestowed pronouns and is violently proud of them while at the same time quite likes experiencing what other pronouns mean." |
| Alan | Two Strand River | Keith Maillard | Genderfluid | 1976 | One of the earliest literary novels to star gender-fluid characters. |
| Alanna of Trebond | The Song of the Lioness | Tamora Pierce | Genderfluid | 1983–1988 | Though the book itself never mentions her to be genderfluid, Pierce said in December 2019 that "Alanna has always defied labels. She took the best bits of being a woman and a man, and created her own unique identity. I think the term is 'gender-fluid', though there wasn't a word for this (to my knowledge) when I was writing her." |
| Annabel | Annabel | Kathleen Winter | Genderfluid | 2010 | Born intersex and assigned male at birth, Wayne sometimes takes on the name "Annabel". |
| The Beetle | The Beetle | Richard Marsh | Genderfluid | 1897 | The villain of the novel, simply called the Beetle, is an Egyptian shapeshifter that appears as both male and female throughout the book. |
| Riley Cavanaugh | Symptoms of Being Human | Jeff Garvin | Genderfluid | 2016 | Riley writes a viral blog about being genderfluid, and struggles to come out to parents and friends, using they/them pronouns often. |
| Chick the Cherub | John Dough and the Cherub | L. Frank Baum | Ambiguous | 1906 | Chick the Cherub is a human child whose gender is ambiguous throughout the book. |
| Crona | Soul Eater | Atsushi Ohkubo | Unknown/Ambiguous | 2010 | Crona is a demon sword master, and they appear as an antagonist under the orders of Medusa for the first part of the series (manga and anime). Their gender is never mentioned throughout the series, however the author explicitly stated that their gender is unknown. In the Japanese text, they are referred to using gender-neutral pronouns. |
| Dust Devil | My Little Pony | Jeremy Whitley | Non-binary | 2020 | Dust Devil is a non-binary abada who uses the singular they/them pronouns. |
| Elliot | On a Sunbeam | Tillie Walden | Non-binary | 2018 | Elliot "Ell" is a non-speaking "mechanical genius" who uses they/them singular pronouns. |
| Alex Fierro | Magnus Chase & the Gods of Asgard | Rick Riordan | Genderfluid | 2015 | Introduced in the second MCGA book, The Hammer of Thor, Alex Fierro is described as "transgender and gender-fluid," going by both masculine and feminine pronouns depending on state of mind and even changing appearance to suit pronouns. |
| The Fool | Realm of the Elderlings Series | Robin Hobb | Genderfluid | 1995–2017 | There is a lot of debate among fans as to what The Fool's biological sex is, but in terms of gender/self identity the Fool canonically identifies as male and female at different times. |
| Oscar François de Jarjayes | The Rose of Versailles | Riyoko Ikeda | Ambiguous | 1972–1973 | A young queer woman raised as a soldier, dressing and behaving as a man, and is regarded as having a masculine presence by other characters. She also sometimes refers to herself by gender neutral pronouns. She is uncomfortable about presenting feminine and wants to be considered as a "genderless god of war" rather than a "gendered" human being. |
| Inanna | The Wicked + The Divine | Kieron Gillen | Non-binary | 2014 | An incarnation of the Sumerian goddess Inanna in the 2014 Recurrence, formerly a teenager called Zahid who had tendency to "blend in". Inanna uses he/him pronouns, except for the last issue in which they use they/them pronouns. |
| Kinetiq | Sovereign | April Daniels | Genderqueer | 2017 | Kinetiq is an Iranian-American genderqueer superhero who has light based superpowers. |
| Leslie | Two Strand River | Keith Maillard | Genderfluid | 1976 | One of the earliest literary novels to star gender-fluid characters. |
| Loki | Loki | Al Ewing | Genderfluid | 2014–present | Takes on both male and female forms, alternating between using he/him and she/her pronouns, and does not feel like he has a gender or orientation. |
| Eleodie Maracavanya | Star Wars: Aftermath | Chuck Wendig | Non-binary | 2015–2017 | A pirate ruler referred to by either male, female or gender-neutral pronouns like "zhe" or "zher". |
| Mogumo | Love Me for Who I Am | Kata Konayama | Non-binary | 2018 | Mogumo is an AMAB non-binary high school student who generally presents femme, and is mistaken for a cross-dresser and invited to work at a cross-dresser maid cafe. |
| Najimi Osana | Komi Can't Communicate | Tomohito Oda | Ambiguous | 2016–present | Described as having an ambiguous/unknown gender/sex by official Japanese website. |
| Niamh | The Kaiju Preservation Society | John Scalzi | Non-binary | 2022 | Niamh is an Irish astronomer and physicist who travels to another dimension populated by kaiju monsters. Niamh is exclusively referred to with singular they pronouns. |
| Paul | Nona the Ninth | Tamsyn Muir | Ambiguous | 2022 | Paul is a fusion of the male Palamedes Sextus and the female Camilla Hect who is referred to with they/them pronouns (whether these pronouns are singular or plural is ambiguous) and whose gender Tamsyn Muir has described as "a clusterfuck". |
| Porcelain | Secret Six | Gail Simone | Genderfluid | 2014–2016 | A new member of the Secret Six. When questioned about their gender presentation, replied "Some days I feel like a girl, other days, not-so-much." |
| Popeye the Sailor | Popeye | E. C. Segar; Doc Winner; Tom Sims; Bela Zaboly; Ralph Stein;Bud Sagendorf;Bobby London;Hy Eisman;R. K. Milholland | Amphibious | 1919–present | In a 1954 comic, Popeye says that he "wears both woman's an man's clothes" and refers to his gender as "amphibious". On International Non-Binary People's Day in 2021, the official Popeye Twitter account posted an image of said comic with the caption "It'sk Nonbinary Day! Happy skelebration to all me amphibious pals!" In another older comic, Popeye describes himself as "both a mother an' father" to his son Swee'Pea." |
| Hero Shackleby | River of Teeth | Sarah Gailey | Non-binary or agender | 2017 | A poisons and demolitions expert and love interest of Houndstooth, Hero goes by singular they/them pronouns, and their gender assigned at birth is never mentioned. |
| Geoffrey Swivel | The Shepherd's Crown | Terry Pratchett | Agender | 2015 | Although raised as a boy, Geoffrey decides that they want to become a witch. When asked why they don't want to be a wizard, Geoffrey tells Tiffany Aching that they don't think of themself as a man, or anything really. They are "merely Geoffrey". |
| Geoffrey Swivel | Tiffany Aching's Guide to Being a Witch | Rhianna Pratchett, Gabrielle Kent | Agender | 2023 | Although raised as a boy, Geoffrey decides that they want to become a witch. When asked why they don't want to be a wizard, Geoffrey tells Tiffany Aching that they don't think of themself as a man, or anything really. They are "merely Geoffrey". |
| Travertine | On the Steel Breeze | Alastair Reynolds | Non-binary | 2013 | Travertine uses "ve/ver" pronouns, and there is no mention of it being unusual in the book. |

## Film

| Character | Title | Portrayed by | Identity | Year | Notes |
| The Adjudicator | John Wick: Chapter 3 – Parabellum | Asia Kate Dillon | Non-binary | 2019 | The character did not have a specified gender in the script; Dillon, a nonbinary person, came up with the idea of making their character nonbinary while talking with the director. |
| All | Zoolander 2 | Benedict Cumberbatch | Androgyne | 2016 | All is presented as a famous androgyne supermodel. The character was subject to a large backlash, being described as "an over-the-top, cartoonish mockery of androgyne/trans/non-binary individuals." |
| A-Spen | Zombies 3 | Terry Hu | Non-binary | 2022 | An unambiguously non-binary space alien. There is a doll for A-Spen, and it will be the first nonbinary doll from Disney. The pronouns on the box are they/them in multiple languages. |
| Biaggio | The Kings of Summer | Moisés Arias | Agender | 2013 | In the film, Biaggio states that he does not see himself as having a gender. |
| Deadeye / Vanessa | Joy Ride | Sabrina Wu | Non-binary | 2021 | Deadeye is Lolo's eccentric cousin who is obsessed with K-pop. |
| Glen/Glenda | Seed of Chucky | Billy Boyd | Genderfluid | 2004 | Although Glen/Glenda was not explicitly identified as genderfluid in the movie, their genderfluid identity was confirmed in the Chucky TV series. |
| Fab G | Cinderella | Billy Porter | Genderless | 2021 | Fab G is the fairy godparent of Ella. |
| Frances | Jimpa | Aud Mason-Hyde | Non-binary | 2025 | Frances travels to Amsterdam with their mother to visit their gay grandfather Jim (known as Jimpa), and wishes to stay with their grandfather for a year. |
| The Happy Medium | A Wrinkle in Time | Seán Cullen | A Happy Medium | 2003 | In the 2003 Disney film A Wrinkle in Time, the protagonists meet the Happy Medium, an exuberant fortune teller. When a character asks if the Happy Medium is a man or a woman, he cheerfully replies "Neither! I'm a happy medium." Although neither male nor female, he is referred to with masculine pronouns in the film. In the original novel, the Happy Medium was portrayed as female, and in the 2018 film adaptation, the Happy Medium was portrayed as male. |
| J | They | Rhys Fehrenbacher | Genderfluid | 2017 | J is a trans teen on puberty blockers that needs to decide their gender before meeting with a doctor. J says they feel male, female, or neither at various times. The actor, Fehrenbacher, was also undergoing gender transition at the time of filming. |
| Jamie | Upgrade | Kai Bradley | Non-gendered | 2018 | A hacker not identifying with any of the genders. Requests that the protagonist not ask their gender, and states that Jamie is not their name and that they do not have a name. |
| S. LaFontaine | The Carmilla Movie | K Alexander | Non-binary | 2017 | In this film, and the web series it serves as a sequel to, LaFontaine uses singular they/them pronouns. |
| Layla | Layla | Bilal Hasna | Non-binary | 2024 | Layla is a British-Palestinian non-binary drag queen who falls in love with the marketing executive Max. |
| Little Horse | Little Big Man | Robert Little Star | Two-spirit | 1970 | Little Horse is a two-spirit Cheyenne indigenous person. |
| Hedwig Robinson | Hedwig and the Angry Inch | John Cameron Mitchell | Genderqueer | 2001 | Hedwig is described by her creator as "more than a woman or a man. She's a gender of one." |
| Frankie Stein | Monster High: The Movie | Ceci Balagot | Non-binary | 2022 | Frankie, the child of Frankenstein and of Dr. Stein is a non-binary monster, made of different male and female body parts, who prefers they/them pronouns. |
| Yivo | Futurama: The Beast with a Billion Backs | David Cross | Non-binary | 2008 | Yivo is a planet-sized alien with no determinable gender, using neopronouns. Yivo dates, then marries all people of the universe at once. Later, they break up. Afterwards, Yivo remains in a relationship with Colleen O'Hallahan. Some have said that Yivo may have been "the first non-binary character defined as such in animated history." |

## Live-action television

| Character | Show title | Portrayed by | Identity | Year | Notes |
| Alex | The A List | Rosie Dwyer | Genderqueer | 2018–present | Uses singular they/them pronouns along with she/her pronouns in the show. |
| Yael Baron | Degrassi: Next Class | Jamie Bloch | Genderqueer | 2016–present | Assigned female at birth, Yael begins to question their identity starting in season 4, before realizing they are genderqueer and uses singular they/them pronouns. |
| Kai Bartley | Grey's Anatomy | E.R. Fightmaster | Non-binary | 2021–present | Uses singular they/them. E.R. Fightmaster is also non-binary and uses they/them pronouns. |
| Bishop | Deputy | Bex Taylor-Klaus | Non-binary | 2020 | Bishop is considered the first non-binary character on broadcast television. |
| Cal Bowman | Sex Education | Dua Saleh | Non-binary | 2021–present | Cal is a non-binary student at Moordale Secondary School, introduced in season 3. The headmistress, Hope Haddon, tries to force Cal to wear the girls' uniform, but Cal repeatedly defies Hope by wearing the boys' uniform instead. Uses singular they/them pronouns. |
| Lindsay Brady | Good Trouble | River Butcher | Non-binary | 2019–present | Uses singular they/them pronouns. |
| Sah Brockner | Casualty | Arin Smethurst | Non-binary | 2021–present | Sah is a non-binary paramedic who uses singular they/them pronouns. |
| Miss Bruce | Star | Miss Lawrence | Genderfluid | 2017 | Bruce is a fierce genderfluid person who became a fan favorite for those who watched the show. |
| Carlos Cervantez | The Winchesters | Jojo Fleites | Non-binary, Bisexual | | Cervantes is a demon hunter. |
| Chris | The Switch | Amy Fox | Non-binary | 2016–present | Chris uses "zie/zir" pronouns in the show. |
| Dane | Fallout | Xelia Mendes-Jones | Non-binary | 2024–present | Dane is a member of the Brotherhood of Steel. |
| Danny the Street | Doom Patrol | (N/A) | Genderqueer | 2019–2022 | Danny is a sentient street who communicates through writing on street signs. They are explicitly referred to as non-binary and genderqueer onscreen. |
| Des | Grown-ish | Egypt Franklin | Queer, Genderfluid, Pansexual, Non-binary | 2021–present | Des, a queer, genderfluid, and pansexual athlete is a possible love interest of the character Jazz. |
| Desire | The Sandman | Mason Alexander Park | Non-binary | 2022 | Like their comics counterpart, Desire is androgynous in appearance and is referred to with gender-neutral language and they/them pronouns, as well as being played by a non-binary actor. |
| Che Diaz | And Just Like That... | Sara Ramirez | Non-binary | | Diaz is a stand-up comic and podcast host. |
| Grencia Mars Elijah Guo Eckener | Cowboy Bebop | Mason Alexander Park | Non-binary | 2021 | Gren is a 29 year old non-binary individual who is connected to Spike and Vicious' past, and is overtly shown as non-binary, as confirmed by Netflix and their voice actor as part of promotional information for the series. Gren describes themselves as "I am both at once, and neither one" in the original anime series the live action is based on, Cowboy Bebop. |
| Brooke Hathaway | Hollyoaks | Tylan Grant | Non-binary | 2018–present | An autistic person who learns about non-binary identities from non-binary friend Ripley Lennox (Ki Griffin). Brooke feels that they relate to the identity. |
| Henchperson Of Indeterminate Gender | A Series Of Unfortunate Events | Matty Cardarople | Non-binary | 2017-2019 | The tie-in book The Incomplete History of Secret Organizations confirms that this version of the character is explicitly non-binary as opposed to having an ambiguous gender in the books. |
| Cassidy Geoffrey | Abbott Elementary | Sabrina Wu | Non-binary | 2021– | Mx. Geoffrey is the new substitute teacher running Janine's classroom. |
| GJ | Top of the Lake | Holly Hunter | Ambiguous | 2013–2017 | GJ is an androgynous Swiss spiritual leader. |
| Jim Jimenez | Our Flag Means Death | Vico Ortiz | Non-binary, Bisexual | 2022–present | Jim uses they/them pronouns, and they are played by a non-binary actor. |
| Jerrie Kennedy | Big Sky | Jesse James Keitel | Non-binary, Transfeminine | 2020–2023 | Kennedy is one of the first nonbinary actors to play a nonbinary series regular on primetime television. |
| S. LaFontaine | Carmilla | K Alexander | Non-binary | 2014–2016 | Uses singular they/them pronouns. |
| Loki Laufeyson | Loki | Tom Hiddleston | Genderfluid | 2021–present | A character from the Marvel Cinematic Universe who first appeared in Thor (2011). Like his comics counterpart and the Norse deity he was based upon, Loki's shapeshifting abilities allow him to change sex at will. In the show, Time Variance Authority paperwork lists Loki's sex as "fluid". |
| Ripley Lennox | Hollyoaks | Ki Griffin | Non-binary | 2020–present | Ripley runs a shop for second-hand clothes and befriends some of the show's younger characters like Peri Lomax (Ruby O'Donnell) and Romeo Nightingale (Owen Warner), while also an established friend of Tom Cunningham (Ellis Hollins). They later come out to their friends as non-binary. |
| Jordan Li | Gen V | London Thor and Derek Luh | Non-binary, Bisexual, Genderfluid | 2023–present | After being injected with Compound V, Jordan is able to switch man and woman genders at will. |
| Sam Malloy | The Riches | Aidan Mitchell | Non-binary, Trans woman | 2007–2008 | Sam, the youngest Malloy child, is transgender and frequently dresses in feminine clothing. The idea for Sam's non-binary gender expression came about before Izzard, a gender non-conforming comedian, joined the show. Sam's gender expression is accepted and respected by the Malloy parents and siblings. |
| Mae Martin | Feel Good | Mae Martin | Non-binary | | Mae comes out as non-binary in the second season. (Actor Mae Martin self-identifies as non-binary.) |
| Taylor Mason | Billions | Asia Kate Dillon | Non-binary | 2016–2023 | A non-binary person who uses singular they/them pronouns and has a storyline centered on a romantic relationship. (2016–present) |
| Sabi Mehboob | Sort Of | Bilal Baig | Genderfluid | 2021–present | They are openly gender fluid and uses they/them pronouns. |
| Mo | Zoey's Extraordinary Playlist | Alex Newell | Genderfluid | 2020–present | He is openly genderfluid, and generally uses he/him pronouns, but is open to the use of any pronouns. |
| Lucifer Morningstar | The Sandman | Gwendoline Christie | Ambiguous | 2022 | The show's version of Lucifer is never referred to with any third-person pronouns and is androgynous in appearance. |
| Pollution | Good Omens | Lourdes Faberes | Non-binary | 2019 | Uses singular they/them pronouns; described by book co-author and series writer Neil Gaiman as non-binary. |
| Joey Riverton | Good Trouble | Daisy Eagan | Non-binary | 2019–present | Joey comes out as nonbinary to their cisgender lesbian girlfriend, Alice, and begins using they/them pronouns. |
| Sam | Vida | Michelle Badillo | Non-binary | 2018–present | Sam's gender identity was not revealed until her sex scene with Emma Hernandez. |
| Syd | One Day at a Time | Sheridan Pierce | Non-binary | 2017–present | Uses singular they/them pronouns. Syd is also the 'syd'nificant other of Elena Maria Alvarez Riera Calderón Leyte-Vidal Inclán, an activist and feminist teenage daughter of Penelope who discovers that she is lesbian and comes out to her family. |
| Adira Tal | Star Trek: Discovery | Blu del Barrio | Non-binary | 2020 | Adira, the first non-binary character in the Star Trek universe, is a highly intelligent character on the U.S.S. Discovery and unexpectedly becomes friends with Lt. Commander Paul Stamets and Dr. Hugh Culber. Adira is also an introvert who does not originally tell the crew they are non-binary, using "she/her" pronouns until episode 8 when Adira comes out as non-binary and asks to be referred to as "they or them." |
| Tam | Younger | Jesse James Keitel | Genderqueer | 2018 | Uses singular they/them pronouns. |
| Lommie Thorne | Nightflyers | Maya Eshet | Genderfluid | 2019 | Lommie is a gender-fluid cyber technician specialist who prefers to interface with computers more than humans. She uses she/her pronouns. |
| Xavin | Marvel's Runaways | Clarissa Thibeaux | Genderfluid | 2019 | A character in the Marvel Cinematic Universe, Xavin is a blunt, romantic alien. In the series, Xavin is referred to with both they/them and she/her pronouns. |
| Zoey | The Switch | Vincent Viezzer | Genderqueer | 2016–present | Zoey is a feisty "transgender genderqueer" woman who is guarded by her neighbor, Detective Sandra McKay, a cisgender lesbian. |

## Theatre

| Character | Title | Original actor | Identity | Premiere | Notes |
| Jerry/Daphne | Some Like It Hot (musical) | J. Harrison Ghee | Non-binary | 2022 | Nonbinary actor J. Harrison Ghee "shows that masculinity, femininity, and anything in between can live alongside each other — and within one person — bringing new-fashioned sensibilities to an old-fashioned Broadway show." |
| May | & Juliet | Arun Blair-Mangat | Agender | 2019 | May is defined as a character who is "not [confined] to any bracket of gender." |
| Mel | How to Dance in Ohio (musical) | Imani Russell | Non-binary | 2023 | Mel introduces themselves as nonbinary and is played by a nonbinary actor. |
| Musidorus | Head Over Heels | Andrew Durand | Genderfluid | 2018 | Comes out by saying that they are both a son and daughter to their mother-in-law. |
| Pythio | Head Over Heels | Peppermint | Non-binary | 2018 | Pythio is a non-binary character. |
| Hedwig Robinson | Hedwig and the Angry Inch | John Cameron Mitchell | Genderqueer | 1998 | Hedwig is described by her creator as "more than a woman or a man. She's a gender of one." |
| Solar | Over and Out: A New Musical | Sushi Soucy | Non-Binary | 2021 | Solar is a student at A New School who is trying to contact aliens after stargazing for years and connects with an alien named Nova on their walkie-talkie, with both later striking up a relationship. The Twitter account for the musical confirmed that Solar is non-binary. |

## Video games

| Character | Game | Voice actor | Identity | Year | Notes |
| Mizuki Akiyama | Hatsune Miku: Colorful Stage | Satou Hinata | Genderqueer | 2020 | In flashbacks, Mizuki is shown with a boy's school uniform and shorter hair. In the present they have long hair and a girl's uniform. When pronouns are needed, they/them pronouns are used, and their gender on their profile is listed as "?". |
| Ash | Wandersong | — | Non-binary | 2018 | Referred to with they/them pronouns in game. The game creator later confirmed they are a nonbinary character. |
| The Bard | Wandersong | John Robert Matz | Non-binary | 2018 | The Bard is referred to with singular they/them pronouns, but it is also mentioned that any pronouns are fine for them in a QA session. |
| Blanche | Pokémon Go | — | Non-binary | 2016 | Referred to using singular they/them pronouns in English-language official media. |
| Bloodhound | Apex Legends | Allegra Clark | Non-binary | 2019 | Bloodhound is referred to as non-binary and uses singular they/them pronouns. |
| Bolt | Crypt of the NecroDancer | — | Genderqueer | 2015 | Bolt is genderqueer, meaning they do not identity "fully as either male or female," according to Ted Martens, artist of this video game. |
| Company Commander | BattleTech | — | Non-binary | 2018 | The Company Commander is the player character. During character creation, the player can define their name, callsign, and pronouns independently of the portrait or background, potentially making them non-binary. Most hired MechWarriors can also be customized in this fashion during the game. |
| Chaos | Hades | Peter Canavese | Non-binary | 2019 | Characters in-game refer to Primordial Chaos with they/them pronouns. Additionally, they were referred to with such pronouns on the official Chaos Update from Supergiant Games' Twitter. |
| Mihaly/Coach of "Rather Be" | Just Dance 2023 Edition | — | Non-binary | 2022 | The coach is referred to with singular they/them pronouns by the official Just Dance Twitter account. The coach is also confirmed to be non-binary in a response to a fan tweet. |
| Alex Cyprin | Astoria: Fate's Kiss | — | Non-binary | 2015 | A boss, with whom characters can engage in a romance with, with gender identity that is accepted by other characters. Uses singular they/them pronouns. |
| Jordan "JD" Davies | Havenfall is For Lovers | — | Non-binary | 2017 | Uses singular they/them pronouns and is non-binary. |
| Kris Dreemurr | deltarune | | Non-binary | 2018 | Throughout both chapters of Deltarune, Kris is referred to by they/them and they have no known gender in the game. |
| Fang | Goodbye Volcano High | Lachlan Watson | Non-binary | 2023 | Referred to with they/them pronouns. |
| Floofty Fizzlebean | Bugsnax | Casey Mongillo | Non-binary | 2020 | Referred to with they/them pronouns and by another character as their "sibling" in game. Developers have confirmed that they are intended to be non-binary representation. |
| FL4K | Borderlands 3 | SungWon Cho | Non-binary | 2019 | Fl4k was confirmed non-binary before the game's release, and is referred to with singular they/them pronouns in-game. They also wear a non-binary pride flag pin. |
| Gargoyle | Starsector | — | Non-binary | 2013 | Gargoyle is never referred to with any pronouns in game dialogue. Starsector writer David Baumgart said that he was "sorta kinda inspired by going out drinking with a couple nonbinary folk". |
| Efrim Waite | Neurocracy | — | Non-binary | 2019 | Waite is always referred as a single individual using they/them pronouns in the in-game encyclopaedia. |
| Cirava Hermod | Hiveswap | — | Non-binary | 2017 | Cirava is referred to with singular they/them pronouns in all official media. |
| Jo | Angry Birds | — | Non-binary | 2023 | Jo, a bird of paradise, was first teased on Angry Birds social media pages on June 3, 2023, for Pride Month. They were included in Angry Birds 2 as a limited-time spell called "Jo's Looove Spell Event". |
| Charun Krojib | Hiveswap | — | Non-binary | 2017 | Charun is stated by What Pumpkin, the production team, to be non-binary, and is referred to with singular they/them pronouns in all official media. |
| Lorelei | Borderlands 3 | Ciarán Strange | Non-binary | 2019 | A soldier and former barista, who is nonbinary, is considering gender transition, but is a non-playable character. |
| Mangle | Five Nights at Freddy's 2 | Jena Rundus | Bigender | 2014 | Other animatronics in the game are clearly gendered. Freddy Fazbear is recognizably male, and Chica the Chicken is recognizably female. Mangle's gender is not discernible, and when asked if Mangle was male or female, Scott Cawthon replied, "Yes." |
| Monster Kid | Undertale | — | Non-binary | 2015 | An enthusiastic, clumsy child, Monster Kid is referred to with singular they pronouns. |
| Napstablook | Undertale | — | "Ghost-gendered" | 2015 | Napstablook is a gloomy ghost who is referred to with singular they pronouns. |
| Nights | Nights into Dreams | — | Agender | 1996 | Franchise developer Takashi Iizuka is cited as saying Nights has no gender, and that players were free to apply their own thoughts on Nights' gender if they wanted to. Nights' voice in Journey of Dreams and Racing Transformed is female, but the games treat them as agender. |
| Nights | Nights: Journey of Dreams | Julissa Aguirre | Agender | 2007 | Franchise developer Takashi Iizuka is cited as saying Nights has no gender, and that players were free to apply their own thoughts on Nights' gender if they wanted to. Nights' voice in Journey of Dreams and Racing Transformed is female, but the games treat them as agender. |
| Nights | Sonic & All-Stars Racing Transformed | Julissa Aguirre | Agender | 2012 | Franchise developer Takashi Iizuka is cited as saying Nights has no gender, and that players were free to apply their own thoughts on Nights' gender if they wanted to. Nights' voice in Journey of Dreams and Racing Transformed is female, but the games treat them as agender. |
| Sackboy | LittleBigPlanet | — | Non-binary | 2008 | While referred to with he/him pronouns, he is confirmed as nonbinary by developer Mark Hall. |
| Setsu | Gnosia | — | Non-binary | 2019 | Refers to themselves as non-binary in the game. |
| Raqio | Gnosia | — | Non-binary | 2019 | Raqio is referred to with singular they/them pronouns. |
| Testament | Guilty Gear | Kayleigh McKee | Agender | 1998 | Was previously referred to with he/him pronouns in the US due to localization issues. As of 2021's Guilty Gear Strive, Testament is now referred to with singular they/them pronouns and is confirmed by the developers to be agender. |
| Siffrin | In Stars and Time | — | Non-binary | 2023 | Is referred to in the game's profile and in the Steam store page with he/they pronouns. There is also an interaction in the game where two characters refer to them as not being a man. |
| Bonnie | In Stars and Time | — | Non-binary | 2023 | Exclusively uses they/them pronouns in both the game's profiles and the Steam store page, and is referred to with gender neutral terms for children such as "the kid" and "preteen" throughout the game. |
| Loop | In Stars and Time | — | Non-binary | 2023 | Loop may have he/they or they/them pronouns. |
| Dominique Pamplemousse | Dominique Pamplemousse | Dietrich Squinkifer | Genderqueer | 2013 | Dominique has a masculine appearance and a feminine voice, leading characters to question their gender. When asked if they are male or female, Dominique replies "I'm a detective." The itch.io page for the second and final game in the series describes Dominique as "our favourite genderqueer private detective". |
| The Pyro | Team Fortress 2 | Dennis Bateman | Ambiguous | 2007 | The Pyro has been referred to with he/him, she/her, they/them, and in language avoiding the use of pronouns. On the game's website, at the end of the character description and after referring to them as a he, it questions if the Pyro is a man. In the captions for Meet the Pyro, a short film made about the character to advertise the game, it shows the Scout saying “He’s not here, is she?”. |

## Webcomics

| Character | Title | Author | Identity | Year | Notes |
| Tilly Birch | Questionable Content | Jeph Jacques | Non-binary | 2003–present | Uses singular they/them pronouns. Character first appeared in 2017. |
| Davepetasprite^2 | Homestuck | Andrew Hussie | Non-binary | 2009–2016 | A fusion of a male character(Dave Strider) and a female character (Nepeta Leijon), Davepetasprite^2 had a short crisis with regards to their gender identity, but quickly settled as non-binary. Character first appeared in 2015. |
| Eth | Eth's Skin | Sfé R. Monster | Gender-neutral | 2014–present | Using singular they/them pronouns in the webcomic, author Sfé Monster has stated that Eth presents and identifies as gender-neutral. |
| Parker Flores | Chroma Key | Brandon Dumas | Non-binary | 2018–2021 (on hiatus) | Parker is in a group of Tokusatsu-inspired heroes. Parker is also non-binary, and some of the narrative conflict comes from characters misgendering them. |
| Calliope | The Homestuck Epilogues ("Meat") | Andrew Hussie | Non-binary | 2009–2019 | Comes out as non-binary in the "Meat" path of The Homestuck Epilogues and uses singular they/them pronouns. Character first appeared in 2012. |
| Roxy Lalonde | The Homestuck Epilogues ("Meat") | Andrew Hussie | Non-binary | 2009–2019 | Comes out as non-binary in the "Meat" path of The Homestuck Epilogues, initially using singular they/them pronouns and later masculine pronouns; in the "Candy" path of The Homestuck Epilogues, Roxy questions her gender, but ultimately continues to identify as female. Character first appeared in 2011. |
| Lucy Marlowe | Never Satisfied | Taylor Robin | Non-binary | 2015–present | Lucy is a non-binary magic apprentice out to prove themselves to an "apathetic master". Uses singular they/them pronouns. |
| Patrick | Strong Female Protagonist | Brennan Lee Mulligan | Genderqueer | 2012–present | Patrick does not identify as a person, although primarily using masculine pronouns. Character first appeared in 2012. |
| Patrick | Strong Female Protagonist | Molly Ostertag | Genderqueer | 2012–present | Patrick does not identify as a person, although primarily using masculine pronouns. Character first appeared in 2012. |
| Rascal | Never Satisfied | Taylor Robin | Non-binary | 2015–present | A magic apprentice, who is non-binary. Uses singular they/them pronouns. |
| R.J. | Paranatural | Zack Morrison | Non-binary | 2010–present | Uses singular they/them pronouns. |
| Ciel Sousa | Serious Trans Vibes | Sophie Labelle | Genderfluid | 2014–present | Ciel is a non-binary girl and seventh grader. Ciel is one of the webcomic's protagonists and Eirikur is her boyfriend. |
| Tetsu | Never Satisfied | Taylor Robin | Non-binary | 2015–present | A magic apprentice, who is non-binary. Uses singular they/them pronouns. |
| Vaarsuvius | The Order of the Stick | Rich Burlew | Genderqueer | 2003–present | Vaarsuvius' gender is deliberately ambiguous, and Burlew has stated that descriptions of them as male or female by other characters do not reflect the reality of the situation. In the commentary of the series' fifth book, Burlew refers to Vaarsuvius as genderqueer, although he has also stated that they would not think of themselves such due to their elvish cultural background. Vaarsuvius also displays difficulty recognizing gender differences in non-elvish characters, and while filling out an application form in the first prequel book, refers to a question about gender whose answer is obscured as "unnecessary". |
| Watch | Go Get a Roomie! | Chloé C | Agender | 2010–present | Watch is comfortable with whichever pronouns the speaker chooses and does not identify with any particular gender. |

## Other

| Character | Medium | Title | Author | Identity | Year | Notes |
| Niko Aris | card game | Magic: The Gathering | Katie Allison, Chris Mooney, Allison Steele, and Lake Hurwitz | Non-binary | 2021 | Introduced in Kaldheim. Niko Aris uses they/them pronouns. |
| Bryce Feelid | web series | Critical Role | Matthew Mercer | Genderfluid | 2018–2021 | Bryce Feelid is a non-binary character introduced in the second campaign of the show; Feelid uses they/them pronouns, as confirmed by Matthew Mercer on Twitter. |
| Hollis | podcast | The Adventure Zone | Griffin McElroy | Non-binary | 2018 | Leader of the Kepler Stunt Club "The Hornets". Hollis used they/them pronouns. |
| Imaginos (Desdinova) | albums | Secret Treaties, Imaginos | Sandy Pearlman, Albert Bouchard | Gender-shifting | 1974, 1988, 2021–present | The character, a shapeshifter, switches between the male identity of Imaginos and the female identity of Desdinova. |
| Marshmallow | web series | Annoying Orange | Dane Boe | Non-binary | 2010–present | A sweet-natured, cheerful, talking marshmallow. Initially, the character refused to specify their gender because it amused them to do so. For twelve years, Marshmallow's gender non-disclosure served as a running gag, but in the episode "PRIDE (Marshmallow's TRUE gender!!!)", they come out of the closet and reveal that their gender is non-binary. |
| Sheriff Sam | podcast | Welcome to Night Vale | Emma Frankland | Non-binary | 2015–present | The new sheriff of Night Vale, Sam is consistently referred to exclusively with they/them pronouns. |
| Zooble | web series | The Amazing Digital Circus | Gooseworx | Zooble | 2023–present | Voiced by Ashley Nichols, Zooble is a jaded human trapped in a digital circus. Their avatar resembles a toy construction set. In the series, Zooble is exclusively referred to with singular they pronouns. Moreover, series creator Gooseworx posted official concept art in which Zooble's gender is classified as "Zooble". |