# List of bisexual characters in animation

This is a list of characters in animation that either self-identify as bisexual or have been identified by outside parties to be bisexual. Listed characters are either recurring characters, cameos, guest stars, or one-off characters. This article also includes characters in Japanese animation, otherwise known as anime. There are also corresponding lists of lesbian and gay animated characters.
For fictional characters in other parts of the LGBTQ community, see the lists of gay, trans, lesbian, non-binary, pansexual, asexual, and intersex characters.
The names are organized alphabetically by surname (i.e. last name), or by a single name if the character does not have a surname. If more than two characters are in one entry, the last name of the first character is used.

## 1960s to 1980s

| Characters | Show title | Character debut date | Notes | Country |
| Rosalie Lamorlière | The Rose of Versailles | October 10, 1979 | She is the adopted daughter of Nicole Lamorlière, attempting prostitution at one point to get money, as shown in the episode "A Beautiful Devil" and swears to kill her mother's murderer in the episodes "The Secret of the Angel" and "Farewell, My Sister!" She tries to kill Oscar's mother but Oscar stops her and soon takes her as an apprentice, earning Rosalie's admiration and love, as she opens his eyes. She later ends up marrying Oscar's friend, Bernard Chatelet in the episode "A Funeral Bell Tolls in the Twilight." | Japan |

## 1990s

| Characters | Show title | Character debut date | Notes | Country |
| Liane Cartman | South Park | August 13, 1997 | In the early seasons, Liane was very sexually active and regularly pursued this activity. She also had no issue with having sexual relations with strangers, usually inviting them to the home as frequent visitors. This desire was not strictly for men, hinted in the episode, "Cartman's Mom Is Still a Dirty Slut" she had intercourse with Ms. Crabtree, Mayor McDaniels, and Sheila Broflovski. | United States |
| Meg Griffin | Family Guy | January 31, 1999 | Throughout the series, Meg has shown romantic interest in and dated several men. However, there have been several instances, in episodes like "Stew-Roids" and "Dial Meg for Murder" in which she has shown hints of being bisexual or a lesbian. In November 2016, when asked about further development on the characters Chris and Meg, Alec Sulkin confirmed there would be a future episode where Meg comes out as a lesbian, taking inspiration from earlier episodes where she exhibited signs of lesbian characteristics. A few episodes since then have hinted at that possible development, such as "The Griffin Winter Games", "Trump Guy", and "Trans-Fat." | United States |
| Stewie Griffin | Family Guy | January 31, 1999 | Stewie has been in relationships with female babies but is also attracted to males. In the commentary for Stewie Griffin: The Untold Story, the writers describe how they were going to make Stewie discover he was gay but decided to scrap this idea in order to retain Stewie's sexual ambiguity for writing purposes. MacFarlane planned for the series' third season to end with Stewie coming out of the closet after a near-death experience. The show's abrupt cancellation, before later continuing, caused MacFarlane to abort these plans, and the episode "Queer Is Stewie?" was produced, but never shown. | United States |
| Anthy Himemiya | Revolutionary Girl Utena | April 2, 1997 | The two leads of the series, Utena Tenjou and Anthy Himemiya, were confirmed to be in love by director Kunihiko Ikuhara. The characters are seen kissing both in the TV show credits and the movie. Utena is also in love with Akio Ohtori as shown in episodes like "The Barefoot Girl", where they also kiss. At the beginning of the series, Anthy is engaged to Kyouichi Saionji, a physically abusive man, who she does not resist and acts as a puppet to obey the current dueling champion, surprised when Utena manages to defeat Saionji, referring to him as a "classmate" after his defeat, as shown in episodes like "The Rose Bride". Later on in the series it is revealed that Akio has been sexually assaulting Anthy. This differs from the manga where Anthy has a consensual relationship with Akio and a romantic relationship with Utena. | Japan |
| Utena Tenjou | Revolutionary Girl Utena | April 2, 1997 | The two leads of the series, Utena Tenjou and Anthy Himemiya, were confirmed to be in love by director Kunihiko Ikuhara. The characters are seen kissing both in the TV show credits and the movie. Utena is also in love with Akio Ohtori as shown in episodes like "The Barefoot Girl", where they also kiss. At the beginning of the series, Anthy is engaged to Kyouichi Saionji, a physically abusive man, who she does not resist and acts as a puppet to obey the current dueling champion, surprised when Utena manages to defeat Saionji, referring to him as a "classmate" after his defeat, as shown in episodes like "The Rose Bride". Later on in the series it is revealed that Akio has been sexually assaulting Anthy. This differs from the manga where Anthy has a consensual relationship with Akio and a romantic relationship with Utena. | Japan |
| Fukiko "Miya-sama" Ichinomiya | Dear Brother | July 14, 1991 | She appears to be calm and generous, even as she dresses conservatively, but is cruel and manipulative, often psychologically and physically torturing Rei for several ambiguous reasons. She desires that Rei only have eyes for her, no one else, as indicated in episodes 5 and 6. She also jealously tries to destroy their relationship by making Nanako love her instead. In the anime, her love for Takehiko is given a backstory: she met and fell in love with him when she was twelve and he spent several days with her in the Ichinomiya mansion, but he unknowingly broke her heart by not showing up to her birthday party. | Japan |
| Nuriko | Fushigi Yûgi | April 20, 1995 | Nuriko initially dresses and acts as a woman named Kang-lin, one of Hotohori's concubines because Kang-lin was his twin sister who died and he wanted to keep her memory alive as shown in the episode, "The Seven Stars of Suzaku." He enjoys cross-dressing, as indicates in the episode "Even If I Die...", and is in love with Hotohori, but later also grows to love the main heroine Miaka as indicated in the episode "Brief Parting." | Japan |
| Akio Ohtori | Revolutionary Girl Utena | July 2, 1997 | Akio Ohtori and Touga Kiryuu were shown in bed together, and Miki described Akio as "seducing" Touga as indicated in the episode "Miki's Nest Box (The Sunlit Garden-Arranged)". Akio and Touga are playboys with a number of female conquests, with Akio's conquests include Utena and Kozue, while Anthy was formerly Touga's girlfriend before Utena came along, which is even shown in the movie. In the light novels, Touga is shown to be engaging in sexual activities with Miki, while in the anime it's implied to have happened. | Japan |
| Touga Kiryuu | Revolutionary Girl Utena | April 2, 1997 | Akio Ohtori and Touga Kiryuu were shown in bed together, and Miki described Akio as "seducing" Touga as indicated in the episode "Miki's Nest Box (The Sunlit Garden-Arranged)". Akio and Touga are playboys with a number of female conquests, with Akio's conquests include Utena and Kozue, while Anthy was formerly Touga's girlfriend before Utena came along, which is even shown in the movie. In the light novels, Touga is shown to be engaging in sexual activities with Miki, while in the anime it's implied to have happened. | Japan |
| Riki | Ai no Kusabi | August 1, 1992 | Riki was once the gang leader of the Bison gang. However he was captured and kept as a pet for three years by Iason who grew obsessively in love with him. She found the characterization of Iason in the third novel to be realistic and compelling, Prior to this, Riki was in a relationship with Guy. He also once engaged in a one-night stand with a female slave named Mimea. | Japan |
| Stephen Stotch | South Park | May 27, 1998 | Despite being married to Linda, Butters' father Stephen Stotch is revealed to be a regular customer at the local gay theater & bathhouse as shown in "Butters' Very Own Episode," and claiming to Linda he only did so out of curiosity, Stephen promises to suppress any future gay urges. However, some episodes, like "Insecurity," show him pursuing these acts in secret. | United States |

## 2000s

| Characters | Show title | Character debut date | Notes | Country |
| Clio Aquanaut | Tactical Roar | January 7, 2006 | She is the player of the crew and is a bisexual, as noted by her voice actress, Rio Natsuki, with many girls after her. She likes to tease and flirt with Hyosuke, as well as anyone else, and is the Weapons Chief. | Japan |
| Franz d'Épinay | Gankutsuou: The Count of Monte Cristo | October 5, 2004 | Franz, a friend of Albert, is engaged to a woman named Valentine for political reasons though he does not love her. He instead heavily implies throughout the series that he has strong feelings for someone else and it is later revealed his feelings are towards his best friend Albert, who is traveling with him. [1] However, he did show some attraction to women and did consider sleeping with a woman in episode 1, "At Journey's End, We Meet". | Japan |
| Ryoji "Ranka" Fujioka | Ouran High School Host Club | June 10, 2006 | Ryoji is Haruhi Fujioka's father who works as a cross-dressing entertainer. In the series, he expresses undying love for his deceased wife, but often brings home male lovers while Haruhi is at school. | Japan |
| Chizu Hanashiro | Sweet Blue Flowers | July 2, 2009 | Fumi's cousin. Chizu and Fumi were very close as children and developed a more physically intimate relationship once they were older. Fumi was in love with Chizu, but Chizu chooses to get married instead, breaking Fumi's heart. Chizu's first child looks a lot like Fumi did as a baby. | Japan |
| Jōji "George" Koizumi | Paradise Kiss | October 3, 2005 | George is a designer at Parakiss. He is bisexual and sees himself as an "equal opportunity lover", including with the protagonist, Yukari "Caroline" Hayasaka as shown in episodes like "Atlier" and "Future", and Isabella previously had a relationship with Joji. | Japan |
| Terry Kimple | The Cleveland Show | October 18, 2009 | Terry, Cleveland's co-worker, marries his husband Paul in one episode, "Terry Unmarried." In the same episode, Cleveland asks if Terry is gay; Terry answers no, much to Cleveland's cheer. But Terry replies he's attracted to both genders, much to Cleveland's dismay. | United States |
| Tomari Kurusu | Kashimashi: Girl Meets Girl | January 18, 2006 | Tomari is Hazumu's childhood friend, so the two know a lot about each other and have many memories from the past. After Hazumu's initial transformation, Tomari does not know what to do. Ayuki observes that Tomari liked Hazumu more as a boy, due to the fact that she has had affections for Hazumu for some time. She ultimately realizes that while Hazumu has changed physically, her personality is still the same, and therefore still the same person inside, resulting in continued romantic attraction. |
| Andrew LeGustambos | Sit Down, Shut Up | April 19, 2009 | Andrew is a flamboyant and bisexual drama teacher whose last name in Spanish roughly translates to "he likes both", a reference to his sexual orientation. He is in love with Larry Littlejunk and Miracle Grohe, with whom he hopes to get into a relationship should they become a couple. | United States |
| Ling-Ling | Drawn Together | October 27, 2004 | Ling-Ling, an Asian trading-card mini-monster, is identified as bisexual by a parody of The Terminator in the episode "Wooldoor Sockbat's Giggle-Wiggle Funny Tickle Non-Traditional Progressive Multicultural Roundtable!" | United States |
| Foxxy Love | Drawn Together | October 27, 2004 | Foxxy, a "sexy mystery-solver," has relations with both men and women, but preferably with the former. She makes out with Princess Clara in the show's first episode, and has a brief BDSM relationship with Captain Hero in another episode, "Requiem for a Reality Show." | United States |
| Linda Memari | American Dad! | June 12, 2005 | Linda is one of the neighbors of the Smith family and is a bisexual Iranian-American woman. Linda saved Francine from the Lady Bugs, a social group for women who cheat on their husbands, by kissing her in the episode "Not Particularly Desperate Housewives." It has been suggested Linda may not be attracted to her husband. In an attempt to hit on Francine, in the episode "Rough Trade," she rearranged her clothes to make her bust more prominent and knocked on the Smiths' door (prompting a drunk Stan to comment "When did you get those?"). After thinking Stan was beating Francine, she made an awkward excuse to leave. Francine then says, "She's a weird chick." Linda's husband is resigned to her preferences, and asks, in a defeated tone, if he can "at least watch this time" when he sees Linda eying Francine. | United States |
| Ione Marcy | Star Wars: The Clone Wars | January 22, 2010 | In February 2016, Pablo Hidalgo, a Lucasfilm creative executive working on the Star Wars franchise, and part of the Lucasfilm Story Group, which had worked on the series, revealed that Cassie Cryar and Ione Marcy, shown together in the episode "Lightsaber Lost," were "written as a couple in a relationship." Both characters only show up in that episode in the series. | United States |
| Pam Poovey | Archer | September 17, 2009 | Pam is the bisexual director of human resources. In a behind-the-scenes feature, her voice actor, Amber Nash described Pam as "a sturdy bisexual". | United States |
| Yukari Sendou | Rosario + Vampire | January 17, 2008 | Yukari (a witchling), has a crush on both Moka (a female vampire) and Tsukune (a male human). Yukari openly wants to have a three-way relationship with them, but Moka and Tsukune don't return her feelings. When Yukari is introduced in both the manga and the anime, she only likes Moka, not Tsukune. Once Moka saves Yukari from being bullied by their monster schoolmates, Yukari confesses her love to her in the second episode, "Witchling and Vampire". At this point, Yukari tries to drive Moka and Tsukune apart, out of jealousy. Only after Tsukune puts himself in harm's way to save Yukari from monsters too, Yukari starts to like him as well and confesses her love to him. | Japan |
| Hatsuharu Sohma | Fruits Basket | September 6, 2001 | Hatsuharu is part of the Sohma family, a family cursed into turning into members of the zodiac when under stress or hugged by members of the opposite sex as shown in the "Make It Clear If It's Black or White" episode. He was born in the year of the cow and was often annoyed by people's comments about the stupidity of the cow from the zodiac, and blamed his troubles on Yuki who was born the year of the rat. However, Yuki helps him realize he needs to move past people's comments and enters an on/off relationship with Rin Sohma, who dumped him in Season 2, he refers to Yuki as his first love. | Japan |
| Coach Daniel Stopframe | Moral Orel | January 30, 2006 | Orel's bisexual coach as well as Shapey's biological father, Daniel lusts after their father, Clay, and at one point has sex with three women and a dog in episodes like "The Blessed Union." | United States |
| Eiri Yuki | Gravitation | October 4, 2000 | Shuichi Shindo falls in love with Eiri Yuki beginning in the first episode. Over the course of the series manages to melt the cold heart of Eiri and the two become a couple. In one episode, "Winding Road", it is revealed that Eiri has a fiancée named Ayaka Usami, but she backs away after seeing the love between Shuichi and Eiri. | Japan |

## 2010s

| Characters | Show title | Character debut date | Notes | Country |
| Maria Akizuki | From the New World | September 29, 2012 | She falls in love with and begins to date Saki but later enters a duty pact with and marries Mamoru Itō. As she is one of the main characters, she appears "ambiguously bisexual." | Japan |
| Shun Aonuma | From the New World | September 29, 2012 | He dated Satoru Asahina for a time but was in love with Saki since childhood. However, he stayed away from Saki for fear of his growing uncontrollable power. As he is one of the main characters, he appears "ambiguously bisexual." | Japan |
| Apollo | Is It Wrong to Try to Pick Up Girls in a Dungeon? | July 31, 2019 | Apollo is bisexual as he has many male members of his familia that he loves and also shows sexual desire towards Bell Cranel, a male adventurer. In addition, when he was in heaven he was besotted with the female goddess Hestia, much to her disgust. | Japan |
| Satoru Asahina | From the New World | September 29, 2012 | Satoru dated Shun for a while but the two later broke up and he began to date another boy for a time, but that relationship also failed. He later falls in love with and marries Saki, shown in the episode "From the New World." Since he is one of the main characters, he appears "ambiguously bisexual." | Japan |
| Princess Bean | Disenchantment | August 17, 2018 | Princess Bean has shown attraction to both men and women. In the premiere of the show, Bean was originally arranged to marry Prince Merkimer. In the season 3 episode "Last Splash", she shares a same-sex kiss with the mermaid Mora. Mora is helpful in getting Bean to Steamland. In the previous season, Bean was shown to enjoy "the company of mermaids." Earlier in the series, Elfo, a male elf, was Bean's love interest. Bean is voiced by bisexual actress Abbi Jacobson. | United States |
| Princess Bubblegum | Adventure Time | April 5, 2010 | Bubblegum is in a relationship with Marceline the Vampire Queen, and may have dated a male character, Mr. Cream Puff. Her exact sexuality, unlike Marcy's, has not been confirmed. As such, reviewers have argued she is either bisexual, non-binary, queer, lesbian, or a combination of some of the latter, as both live in a world where "sexuality is somewhat fluid." | United States |
| Blake Belladonna | RWBY | July 25, 2013 | In a conversation with the voice actresses for Ruby Rose (Lindsay Jones), Weiss Schnee (Kana Eberle), Yang (Barbara Dunkelman), and Patty Hawkins, for GalaxyCon, the voice actress for Blake, Arryn Zech, confirmed that Blake likes men and women, with the other cast members concurring with her assessment. She had a previous relationship with her abusive boyfriend, Adam Taurus. She gets along well with her teammate, Yang Xiao Long. Fans and reviewers either shipped them as "Bumbleby" after the episode "Burning the Candle", or noted their growing relationship over the show's seasons. At the end of the show's sixth season, Yang works with Blake to kill Adam Taurus, and her romantic feelings were "strongly hinted". She also is close to Sun Wukong in other episodes. In the Volume 9 episode "Confessions Within Cumulonimbus Clouds", Blake and Yang accept their feelings for one another and kiss. Some reviewers also noted romantic subtext between both characters in RWBY: Ice Queendom. | United States. |
| Jay Bilzerian | Big Mouth | September 29, 2017 | He has shown various moments of being sexually attracted to women as well as Matthew, even sharing a kiss with one another after Jay stated the phrase, "Hey, man, a mouth's a mouth". | United States |
| Bow | She-Ra and the Princesses of Power | November 13, 2018 | In a Twitch stream on June 9, 2020, Molly Ostertag stated she doesn't believe Bow or Glimmer are heterosexual, a sentiment series creator ND Stevenson concurred with, saying they are "definitely not straight." In the last episode of the show's final season, "Heart Part 2," Bow and Glimmer kissed, making their relationship canon, while Adora imagines Bow and Glimmer in a long-term relationship, possibly even married. Some have said Bow and Glimmer are bisexual, which would imply Glimmer had a crush on Adora or Bow had a crush on Seahawk, while others have stated Bow is trans after he wore a binder in the episode "Shadows of Mystacor." The latter is a fan interpretation that Stevenson is "very fond of." | Japan |
| Glimmer | She-Ra and the Princesses of Power | November 13, 2018 | In a Twitch stream on June 9, 2020, Molly Ostertag stated she doesn't believe Bow or Glimmer are heterosexual, a sentiment series creator ND Stevenson concurred with, saying they are "definitely not straight." In the last episode of the show's final season, "Heart Part 2," Bow and Glimmer kissed, making their relationship canon, while Adora imagines Bow and Glimmer in a long-term relationship, possibly even married. Some have said Bow and Glimmer are bisexual, which would imply Glimmer had a crush on Adora or Bow had a crush on Seahawk, while others have stated Bow is trans after he wore a binder in the episode "Shadows of Mystacor." The latter is a fan interpretation that Stevenson is "very fond of." | Japan |
| Dio Brando | JoJo's Bizarre Adventure | October 5, 2012 | Dio is canonically bisexual in both the anime and manga. In the 2007 Eureka interview with Araki (the author of the franchise), when asked about Dio's sexuality, Araki responded: "...Dio's sort of a composed character that could go either way. He could go with a man or a woman." Dio's sexuality is incidental to his villainy. | Japan |
| Reiner Braun | Attack on Titan | April 14, 2013 | In Chapter 38 of the manga and Episode 4 Season 2 of the anime, there was a conversation between Ymir and Reiner, in which Ymir said that Reiner "doesn’t seem like the kind of guy who is interested in women...", to which Reiner replied in the affirmative and added that Ymir does not look like a girl who is interested in guys either. Considering that Ymir is a lesbian, this is actually a double coming out of the characters to each other. The character description in the DVD booklet also indicates that Reiner has a "high BL index", the only one of the male characters. Despite that, Reiner has shown some interest in Historia several times throughout the story, although he never told her about it. In the context of his split personality, his sexuality becomes even more complex. | Japan |
| Clarence | Final Space | February 15, 2018 | A recurring character, he has an ex-wife, several since-deceased wives, and two adopted children (Fox and Ash Graven). He also is implied to be in love with General Cataloupe and has unrequited love on Sheryl Goodspeed. Series creator Olan Rogers said that he "always saw Clarence as a Bi-Sexual." | United States |
| Clarence | Sword Art Online Alternative Gun Gale Online | June 9, 2018 | In episode 9, Clarence, a fellow GGO player, is dying and Karen demands Clarence give up her remaining magazines. Clarence demands a kiss in exchange and reveals she is female (and chose an androgynous avatar) and that she is bisexual. | Japan |
| Wendy Corduroy | Gravity Falls | June 15, 2012 | In August 2020, show creator Alex Hirsch implied that Wendy is bisexual in a tweet. Wendy is a mellow, tomboyish, laid-back 15-year-old part-time employee at the Mystery Shack, and she stated that she had many boyfriends in the past, including one ex-boyfriend with whom she cannot remember breaking up. | United States |
| Jeremy De Longpre | Allen Gregory | October 30, 2011 | Richard and Jeremy are the fathers of the title character. Jeremy is a former social worker who had a loving wife and family, although this changed after Richard became one of his clients. Richard was attracted to Jeremy to the point where he started stalking him and his family until Jeremy finally agreed to be his husband. Jeremy left his wife and children for Richard, who offered him an easy, no-maintenance life as his trophy husband. | United States |
| Enid | OK K.O.! Let's Be Heroes | August 1, 2017 | Enid is a witch and ninja who loves Red Action. In the episode, "We Messed Up" when Enid sees Mr. Gar's photo of KO's young mom, she eagerly asks who the "babe" is. Also, when Enid and K.O. are biking to Rad's house in "Hope This Flies", Enid's helmet has a peace sign sticker colored as the bisexual pride flag. Enid also has possibly romantic feelings toward Elodie. Red Action and Enid shared a kiss at the end of the episode, "Red Action 3: Grudgement Day", in the show's final season. | United States |
| Entrapta | She-Ra and the Princesses of Power | November 13, 2018 | Entrapta, princess of Dryl, is a skilled but reckless inventor. She is one of the most knowledgeable people on First Ones' tech in Etheria. Entrapta first sees Hordak, head of the Horde, as her work partner, and reunites with him in the show's last season, with both walking off together in the show's final episode. Their relationship was confirmed as canon by series creator ND Stevenson and character designer Rae Geiger. According to Stevenson, Entrapta "sees humanity in everything" and had "lots of robot boyfriends and girlfriends," along with her relationship with the ship, Darla, in "some capacity." | United States |
| Ertegun | Carole & Tuesday | April 10, 2019 | In episode 2, Ertegun states that he has love "only for capable dudes and great chicks". | Japan |
| Sleepy Gary | Rick and Morty | August 16, 2015 | In "Total Rickall", Sleepy Gary is one of the dozens of aliens that alter the memories of Rick, Morty, Beth, and Summer in order to pose as friends and family members. Because of the fake memories, Beth believes herself to be married to Sleepy Gary, and Jerry believes he is having a secret affair with him. | United States |
| Halo | Young Justice | January 4, 2018 | Halo's first love interest is Geo-Force/Brion, a male character, and they declare at a meeting that they "do not know that I am a girl or a boy, as Earth languages define the word.". Later, Halo kisses a female character, Harper Row, who is also bisexual, in the third season of Young Justice. | United States |
| Akihiko Kaji | Given | July 11, 2019 | Akihiko has a romantic history with both men and women. He eventually falls in love with the leader of his band, Haruki Nakayama, himself a bisexual man, and starts to date him. | Japan |
| Yuri Katsuki | Yuri On Ice | May 26, 2017 | Yuri and Victor get engaged giving each other gold rings In episode 1, Yuri expresses a former crush he had on his female friend Yuuko. | Japan |
| Yuzu Kichougasaki | Love Tyrant | April 6, 2017 | She has a crush on her half-sister, Akane Hiyama, but later in the series she develops feelings for Seiji Aino, as indicated in the show's ninth episode. | Japan |
| Kyoshi | The Legend of Korra | June 23, 2012 | Michael Dante DiMartino, one of the series creators, in an interview with EW, noted that while the show's crew had always believed she was bisexual, her feelings toward women and men were actually only explored in the young adult novel and in the comics branching off of the series. | United States |
| Marie Logan | Young Justice | November 26, 2010 | Marie Logan is introduced in the first season as the mother of Garfield Logan / Beast Boy. In a tie-in comic, Queen Bee uses her control of pheromones to enthrall Marie, with her abilities only working on people that are sexually attracted to women. This means Marie was either a lesbian or bisexual and her powers tied to her sexual attraction. | United States |
| Lagoon Boy | Young Justice | March 4, 2011 | La'gaan first appeared in season 1. Lagoon Boy then appeared in season 2 as the jealous boyfriend of Miss Martian. In season 4, he's happily married to both a man and a woman. | United States |
| Luna Loud | The Loud House | May 2, 2016 | Luna shows attraction to Hugh, her brother's tutor, in "Study Muffin", and sends a love letter to a girl named Sam in "L is for Love". Sam seems to feel similarly about Luna, as shown in the latter episode. Sam Sharp appears to reciprocate Luna's feelings in that episode and others, with some describing them as beginning to date in the episode "Racing Hearts." However, in June 2021, Kevin Sullivan, a story editor for The Loud House told Insider that Luna was intended to a lesbian, but said that he was glad the team didn't push it further because Luna then becomes "representative of so many more young people struggling with their identity," implying that fan interpretations of her as bisexual was still valid. | United States |
| Lukkage | Gargantia on the Verdurous Planet | April 21, 2013 | She has two female sex slaves who also serve as her co-pilots for her mecha, as shown in the episode "The Villainous Empress" and other episodes. Later she develops a romantic interest in Pinion. In the two OVAs for the show, "Far Beyond the Voyage" parts 1 and 2, Lukkage is a former pirate involved with salvaging and guarding Gargantia from threats, with her two companions still by her side, helping her run the ship and gather intel. | Japan |
| Ash Lynx | Banana Fish | July 5, 2018 | Ash has slept with men and women during his time as a prostitute. He mainly has relationships with men such as Eiji, while dealing with childhood trauma from molestation. While has a close relationship with Eiji, he admitted in one episode that he had a relationship with a girl who was killed "under suspicion of being his girlfriend." | Japan |
| Honoka Maki | Kiznaiver | April 10, 2016 | Honoka is in love with a girl named Ruru and wants a romantic relationship with her, as shown in the show's sixth and seventh episodes. Maki later has feelings for a male friend named Yuta and their attractions toward each other are reciprocal. | Japan |
| Yumi Mamiya | MM! | October 9, 2010 | She hates Taro and loves Tatsukichi, but does not know that Tatsukichi is a cross-dresser, and in other scenes, it is implied that she has lesbian tendencies when groping and fondling Arashiko and Mio. | Japan |
| Marie | Carole & Tuesday | May 1, 2019 | Marie used to date Gus, but now is in a relationship with Anne, and the two plan to get married soon, as shown in episode 4. | Japan |
| Marceline the Vampire Queen | Adventure Time | May 17, 2010 | Marceline is bisexual as she dated a male wizard named Ash, and in the series finale, shared an on-screen kiss with Princess Bubblegum, confirming their relationship. The latter had been hinted at and subtle since the episode "What Was Missing," leading fans to ship these characters. Additionally, Marceline's VA, Olivia Olson confirmed that Marceline and Princess Bubblegum had dated. In Adventure Time: Distant Lands, the episode "Obsidian" focused on Marceline and Bubblegum's relationship. | Japan |
| Charlie Morningstar | Hazbin Hotel | October 28, 2019 | Charlie is described as "gay" by news anchor Katie Killjoy, while she is shown as being in a relationship with Vaggie in the pilot episode. Charlie was implied to be bisexual based on the colors of the bisexual pride flag appearing in a tweet from her account, describing Charlie and Vaggie's intimate relationship as to that of Jack Skeleton and Sally's relationship from The Nightmare Before Christmas. | United States |
| Moxxie | Helluva Boss | November 25, 2019 | Moxxie, the protagonist of Helluva Boss, is a demon, assassin, and weapons expert of I.M.P. On Bi Visibility Day, in September 2020, series creator Vivienne Medrano revealed he is bisexual on her Twitter account. In the season 2 episode "Exes and Oohs", Moxxie explicitly states to his father that he is bisexual. | United States |
| Yuma Okazaki | NTR: Netsuzou Trap | July 5, 2017 | The story centers around two high school girls named Yuma and Hotaru who each have a boyfriend but secretly cheat with each other. Yuma cannot explain the feeling she gets around Hotaru, which eventually leads her to believe that their relationship may be more than just a friendship. In the final episode, "Why Did It Take Me This Long to Realize?", Yuma finally realizes that Hotaru has had feelings for her all along and confesses her love for her. | Japan |
| Hotaru Mizushina | NTR: Netsuzou Trap | July 5, 2017 | The story centers around two high school girls named Yuma and Hotaru who each have a boyfriend but secretly cheat with each other. Yuma cannot explain the feeling she gets around Hotaru, which eventually leads her to believe that their relationship may be more than just a friendship. In the final episode, "Why Did It Take Me This Long to Realize?", Yuma finally realizes that Hotaru has had feelings for her all along and confesses her love for her. | Japan |
| Pitohui "Pito" | Sword Art Online Alternative Gun Gale Online | April 22, 2018 | In episode 12, after revealing that she is Elsa Kanzaki, she kisses the main character Karen on the lips and flirts with her. M/Goshi Asogi, who is her manservant and in love with her, warns Karen that Elsa goes through men and women relatively quickly. | Japan |
| Plum | Bravest Warriors | January 20, 2013 | Plum had a crush on Chris, kissing him multiple times during the show in the episodes "Gas-Powered Stick," "Hamster Priest" and "Merewif Tag," even as she rejected advanced from Danny and Wallow in the episode "Mexican Touchdown." She is also madly in love with her doppelganger, as shown in the canonical comics while she entered a relationship with a female character named Peach in another comic. Furthermore, on numerous occasions, she has identified as bisexual, confirmed by Kate Leth, who wrote all three volumes of the Bravest Warriors comic, writing "Plum is bi and it's canon and I'm proud of her." | United States |
| Plum | Bravest Warriors | January 20, 2013 | Plum had a crush on Chris, kissing him multiple times during the show in the episodes "Gas-Powered Stick," "Hamster Priest" and "Merewif Tag," even as she rejected advanced from Danny and Wallow in the episode "Mexican Touchdown." She is also madly in love with her doppelganger, as shown in the canonical comics while she entered a relationship with a female character named Peach in another comic. Furthermore, on numerous occasions, she has identified as bisexual, confirmed by Kate Leth, who wrote all three volumes of the Bravest Warriors comic, writing "Plum is bi and it's canon and I'm proud of her." | Canada |
| Harley Quinn | Harley Quinn | November 29, 2019 | Harley and Ivy are best friends. After much buildup to Harley and Ivy's relationship, in the seventh episode of the show's second season, "There's No Place to Go But Down," they both kiss after Harley saved Ivy, following their escape from prison. This episode happened after the first season began a "slow burn journey of self-discovery for both of the troubled yet powerful women" in the words of one reviewer. | United States |
| Poison Ivy | Harley Quinn | November 29, 2019 | Harley and Ivy are best friends. After much buildup to Harley and Ivy's relationship, in the seventh episode of the show's second season, "There's No Place to Go But Down," they both kiss after Harley saved Ivy, following their escape from prison. This episode happened after the first season began a "slow burn journey of self-discovery for both of the troubled yet powerful women" in the words of one reviewer. | United States |
| Harper Row | Young Justice | January 25, 2019 | Harper Row is the bisexual friend to Violet Harper and Fred Bugg. In the episode, "Early Warning," she drunkenly kissed Violet even though both of them have boyfriends. In the original comics, she explicitly refers to herself as bisexual. | United States |
| Asami Sato | The Legend of Korra | April 28, 2012 | In The Legend of Korra, both Asami and Korra are romantically involved with Mako at different points. The series finale, "The Last Stand," ended with Korra and Asami holding hands and looking into each other's eyes while traveling through a portal right before the ending credits. The creators later confirmed the intention of the ending scene was to show Asami and Korra becoming a romantic couple. In the graphic novel The Legend of Korra: Turf Wars, which is the sequel to the animated series, Korra and Asami are in a relationship. | United States |
| Korra | The Legend of Korra | April 14, 2012 | In The Legend of Korra, both Asami and Korra are romantically involved with Mako at different points. The series finale, "The Last Stand," ended with Korra and Asami holding hands and looking into each other's eyes while traveling through a portal right before the ending credits. The creators later confirmed the intention of the ending scene was to show Asami and Korra becoming a romantic couple. In the graphic novel The Legend of Korra: Turf Wars, which is the sequel to the animated series, Korra and Asami are in a relationship. | United States |
| Izumi Sena | Love Stage!! | July 9, 2014 | Ryoma is a 20-year-old popular actor and hard worker, while Izumi is an 18-year-old geeky university student. The two fall in love despite Izumi initially not being comfortable with the idea of being in a relationship with another man and Ryoma struggling to accept his feelings despite having previously only liked women as shown in the episode "The Door to My Dreams" and others. | Japan |
| Ryoma Ichijo | Love Stage!! | July 9, 2014 | Ryoma is a 20-year-old popular actor and hard worker, while Izumi is an 18-year-old geeky university student. The two fall in love despite Izumi initially not being comfortable with the idea of being in a relationship with another man and Ryoma struggling to accept his feelings despite having previously only liked women as shown in the episode "The Door to My Dreams" and others. | Japan |
| Suzuka | Akame ga Kill! | November 2, 2014 | She is bisexual, having shown enjoyment at being beaten by a group of spies and showing sexual interest in Esdeath and Tatsumi, indicated in episodes like "Kill the Fate". | Japan |
| Kanade Suzutsuki | Mayo Chiki! | July 7, 2011 | She notes that Kinjirō is the first, and only, man who is willing to talk her down and she has fallen in love with him as a result. She is also implied to be a bisexual as she has claimed that Subaru was her first love. | Japan |
| The Nines | Darling in the Franxx | March 31, 2018 | Although their piloting system is also a "couple" of a boy and a girl, throughout the show their squad is positioned as an "alternative" to traditional heterosexual relationship, and the leader of the Nines even several times lectures on the meaninglessness of gender differences. Previously, he also openly notes his bisexuality, being attracted not only to Ichigo, but also to Hiro as a "cute boy". | Japan |
| Jackie Lynn Thomas | Star vs. the Forces of Evil | January 18, 2015 | Jackie-Lynn and Marco start dating in the episode "Bon Bon the Birthday Clown", but ended their relationship in the episode "Sophomore Slump" due to Marco's feelings for Star. In the episode "Britta's Tacos", it is revealed Jackie has a relationship with a French girl named Chloé. | United States |
| Professor Venomous | OK K.O.! Let's Be Heroes | August 17, 2017 | Ian Jones-Quartey confirmed Professor Venomous is bisexual, when asked by a fan, but is not non-binary. | United States |
| Saki Watanabe | From the New World | September 29, 2012 | She loved Shun for a long time but dates Maria and later marries Satoru, shown in the episode "Omen." Since she is one of the main characters, she appears "ambiguously bisexual." | Japan |
| Sayaka "Ayaka" Watanabe | The Kawai Complex Guide to Manors and Hostel Behavior | April 3, 2014 | Ayaka is a college student who loves to lead men on but reveals later on that she dates both men and women and loves reading BL in the episode "Thought So". She also is shown to have a sexual attraction to her fellow dormmate Mayumi, whom she often fondles despite the latter's resentment. | Japan |
| Sasha Waybright | Amphibia | June 14, 2019 | Sasha is Anne and Marcy's friend who got transported to Amphibia with them. In the series finale episode "The Hardest Thing", Sasha is shown to have a bisexual pride sticker on the interior mirror of her car. Immediately after the episode aired, series creator Matt Braly stated directly in a tweet that the character was bisexual. Sasha is voiced by bisexual actress Anna Akana. | United States |
| Charteux "Char" Westia | Boarding School Juliet | October 13, 2018 | The male protagonist, Romio, discovered that Char was in love with Juliet, the female protagonist, in the episode "Romio and Princess Char". | Japan |
| Takafumi Yokozawa | The World's Greatest First Love | May 21, 2011 | Takafumi claims to have liked women in the past, but fell in love with Masamune Takano who did not reciprocate his feelings. He later enters a relationship with Zen Kirishima, who was once happily married until his wife tragically passed as shown in the episode "Actions Speak Louder Than Words", as shown in the movie connected to the franchise. | Japan |
| Zen Kirishima | The World's Greatest First Love | May 21, 2011 | Takafumi claims to have liked women in the past, but fell in love with Masamune Takano who did not reciprocate his feelings. He later enters a relationship with Zen Kirishima, who was once happily married until his wife tragically passed as shown in the episode "Actions Speak Louder Than Words", as shown in the movie connected to the franchise. | Japan |
| Kou Yukina | The World's Greatest First Love | March 22, 2011 | Shouta Kisa has a bad habit of falling for good looking men and falls for Kou, but is scared to pursue a relationship as he feels love does not really exist. Kou dated women in the past but later falls in love with Shouta and the two develop a strong romantic relationship. | Japan |
| Zess (Luka Corosszeira) | The Betrayal Knows My Name | April 11, 2010 | Yuzuki's previous incarnation was a woman named Yuki, who was in love with a man named Luka. In the present, Yuki, now in a male form, is highly protective of Luka, and vice versa, with both highly protective of each other, with implied romantic ties between them. | Japan |

## 2020s

| Characters | Show title | Character debut date | Notes | Country |
| Sophia Ascart | My Next Life as a Villainess: All Routes Lead to Doom! | April 18, 2020 | In the eighth episode, when inside the book world, Sophia pins Catarina against a wall, confesses her love, and proposes they move in together. Although Catarina does not understand the ramifications of this confession, Maria and Mary, who also have crushes on Catarina, recognize it full well. In the ninth episode, "Things Got Crazy at a Slumber Party...," Anne Shelley observes that Sophia tries to get Catarina to realize that she has feelings for her, but to no avail. Sophia also has feelings for men as well, and her brother is Nicol. | Japan |
| Daphne Blake | Velma | January 12, 2023 | Daphne Blake is portrayed as an Asian foundling who has a crush on Velma Dinkley. She dated Fred Jones until they broke up. Velma and Daphne kissed in the second episode "The Candy (Wo)man". She also has two mothers Donna and Linda Blake. | United States |
| Maria Campbell | My Next Life as a Villainess: All Routes Lead to Doom! | April 25, 2020 | When Catarina saves Maria from bullies, in the episode "I Enrolled in the Magic Academy...," she steals a romantic event from Geordo, causing Maria to begin falling love with Catarina in the fourth episode of the series. In the following episode, Catarina steals a major romantic scene from Keith unintentionally, and they grow closer together. In a later episode, "Things Got Crazy at a Slumber Party...," Anne Shelley says that Maria shows her love for Catarina by sharing her sweets with her. In the episode "The Moment of My Doom Arrived... Part 1," Maria defends Catarina from false charges and says that Catarina means a lot to her. In the show's season one finale, Catarina asks her who she likes, and she says "the only one I love, admire, and want to be with for all time is you, Lady Catarina. So please allow me to stay by your side from now on." Maria likely has feelings for men as well, as her line she tells Catarina was supposed to be spoken to a man in the original game. | Japan |
| Velma Dinkley | Velma | January 12, 2023 | While Velma initially had a crush on Daphne's former boyfriend Fred, she is receptive to Daphne's romantic feelings towards her. They also kissed in the second episode "The Candy (Wo)man" | United States |
| Laura Feinberg | Little Demon | August 25, 2022 | Laura Feinberg is Chrissy's mother and Satan's ex-lover. In the episode "Wet Bodies", Laura is revealed to be bisexual as she formerly had a same-sex relationship with Sea Hag. Laura is voiced by bisexual actress Aubrey Plaza. | United States |
| Mary Hunt | My Next Life as a Villainess: All Routes Lead to Doom! | April 11, 2020 | She develops romantic feelings toward the series protagonist, Catarina, differing from the script of the otome game, Fortune Lover, beginning in the second episode of the series, "A Prince Challenged Me To a Fight..." In the ninth episode, "Things Got Crazy at a Slumber Party...," Anne Shelley, Catarina's maid, observes that Mary loves Catarina so much that she concocted a "terrifying plan" of sorts to get them to stay together. In the same episode, Mary, in a sleepover with Catarina, Sophia, and Maria, admits she likes Catarina, saying she and someone she loved would trade dresses. Her fiancé is Alan. | Japan |
| Norma Khan | Dead End: Paranormal Park | June 16, 2022 | Norma is Barney's Pakistani-American, autistic best friend. Although she was assumed to be pansexual, due to an illustration by series creator Hamish Steele showing her holding the pansexual pride flag, he later clarified in a Twitter direct message that she likes multiple genders but initially did not identify with any specific label. She comes out as bisexual in the second season. She has a crush on Badyah Hassan, her friendly, sarcastic friend of Iranian descent who works at Dead End with her and seemingly returns her feelings. However, Badyah turns her down in the second season as she is straight. | United States |
| Suletta Mercury | Mobile Suit Gundam: The Witch from Mercury | October 2, 2022 | Initially showing attraction to men and not knowing about the idea of same-sex relationships, Suletta Mercury, the main character, gradually develops mutual romantic feelings for Miorine, the girl whose fiancé she accidentally becomes. Although the show mostly tries to keep it within the subtext, in the end, both girls in one way or another declare a clear desire to actually get married and are shown as spouses with rings on their ring fingers at the end of the story. The status of the two girls as a married same-sex couple was also confirmed by their voice actresses on the final stream on the day of the show's final episode. Thus, Suletta becomes not only the official first female, but also the first LGBTQ protagonist in a franchise. | Japan |
| Nico Minoru | Your Friendly Neighborhood Spider-Man | January 29, 2025 | In this series, Nico Minoru is portrayed as Peter Parker's best friend. As in the comics, like Runaways, she is bisexual, teasing Peter that she will ask out his crush, Pearl Pangan, if he doesn't do so, and she is, also, a love interest for Peter. | United States |
| Luz Noceda | The Owl House | January 10, 2020 | Luz Noceda, the main character of The Owl House, has shown explicit interest in men and women. As shown in the second episode, "Witches Before Wizards" Luz meets a character named Nevareth, who she reacts to in a way similar to that of a small crush or infatuation. In the episode "Lost In Language", she's shown to be blushing at both Edric and Emira Blight. A few episodes later, in "Adventures in the Elements", she states, "I've got a new crush, and her name is education". In the episode "Knock, Knock, Knockin' on Hooty's Door", she and Amity Blight officially become girlfriends. In season 3 episode Thanks to Them," Luz is shown coming out to her mom during a montage, and wears a bisexual pride pin for much of the episode. Dana Terrace, the series's creator, confirmed on Twitter she wanted to make a bisexual character. On September 2, during a Reddit AMA, Terrace official confirmed Luz is bisexual. She also stated Luz is "oblivious to some things in front of her," including Amity's crush on her. Luz is the first bisexual lead character on a Disney Channel show. | United States |
| Graham Prescott | Rugrats | May 21, 2021 | Graham Prescott is a flamboyant aging hippy. He first appeared in the first episode "Second Time Around" in which he is one of Grandpa Lou's dates from the seniors-only dating app Silver Beagles that Angelica only used to get cookies and sweets out of elderly people. Graham is shown to be romantically interested in Lou. Lou turns him down but they both become friends. In the holiday episode "Traditions", Graham is interested in Betty's aunt Tia Esperanza. | United States |
| Vex'ahlia "Vex" de Rolo | The Legend of Vox Machina | January 28, 2022 | Vex'ahlia and Vax'ildan are half-Elf twin siblings and members of the Vox Machina team. Both siblings are bisexual. | United States |
| Vax'ildan "Vax" Vessar | The Legend of Vox Machina | January 28, 2022 | Vex'ahlia and Vax'ildan are half-Elf twin siblings and members of the Vox Machina team. Both siblings are bisexual. | United States |
| Saki Saki | Girlfriend, Girlfriend | July 3, 2021 | She dates her childhood friend and classmate, Naoya Mukai, but their classmate, Nagisa Minase, also confesses her feelings to Naoya. After some initial hesitation, Naoya accepts Nagisa's request to be her boyfriend as well. Naoya decides that he will date both Saki and Nagisa at the same time. Since the first episode of the first season of the anime, Saki displays "signs of bi-panic" with hints at her bisexuality. | Japan |
| Melissa Tartleton | M.O.D.O.K. | May 21, 2021 | Melissa Tartleton is M.O.D.O.K's 17-year-old daughter who shares her father's appearance. Melissa is a popular girl who wants to be a supervillain. She is also openly bisexual. While her sexuality is not remarked upon in the first season. It was planned to be explored in season 2 according to Melissa's voice actor Melissa Fumero. However the series was cancelled before that could happen. | United States |
| Tulip | Oh My God…Yes!: A Series of Extremely Relatable Circumstances | March 9, 2025 | In the second episode, a robot girl named Strawberry breaks up with her after she slept with a man named Silken Tofu boy, and because she is overprotective, saying she loves her, but she doesn't feel safe around her, causing Tulip to lose it. She becomes involved with a male spider, which she calls "Rutherford", afterward. She is also friends with Ladi and Sunny. | United States |