OpenLLM-France/wikimedia huggingface dataset:
# Pfu DNA polymerase

Pfu DNA polymerase is an enzyme found in the hyperthermophilic archaeon Pyrococcus furiosus, where it functions to copy the organism's DNA during cell division (thermostable DNA polymerase). In the laboratory setting, Pfu is used to amplify DNA in the polymerase chain reaction (PCR), where the enzyme serves the central function of copying a new strand of DNA during each extension step.
It is a family B DNA polymerase. It has an RNase H-like 3'-5' exonuclease domain, typical of B-family polymerase such as DNA polymerase II.

## Proofreading ability of Pfu polymerase

Pfu DNA polymerase has superior thermostability and proofreading properties compared with Taq DNA polymerase. Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity, meaning that as the DNA is assembled from the 5' end to 3' end, the exonuclease activity immediately removes nucleotides misincorporated at the 3' end of the growing DNA strand. Consequently, Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts.
Commercially available Pfu typically results in an error rate of 1 in 1.3 million base pairs and can yield 2.6% mutated products when amplifying 1 kb fragments using PCR. However, Pfu is slower and typically requires 1–2 minutes per cycle to amplify 1kb of DNA at 72 °C. Using Pfu DNA polymerase in PCR reactions also results in blunt-ended PCR products.
Pfu DNA polymerase is hence superior to Taq DNA polymerase for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with Taq polymerase to obtain the fidelity of Pfu with the speed of Taq polymerase activity.

## History

Scientists led by Eric Mathur at the biotech company Stratagene, based in La Jolla, California, discovered Pfu DNA polymerase which exhibits significantly higher fidelity of replication than Taq DNA polymerase in 1991. They received patents for exonuclease-deficient Pfu and the full Pfu in 1996.
Other polymerases from Pyrococcus strains such as "Deep Vent" (Q51334) from strain GB-D and Pwo DNA polymerase have also seen use.
# Charles Jeanne

Charles Jeanne (15 May 1800 – 11 July 1837) was one of the leaders of the Parisian June Rebellion in 1832. He died of tuberculosis in 1837.
His memoir À Cinq Heures Nous Serons Tous Morts (At Five O'Clock We Will All Be Dead) helped to inspire Victor Hugo and the barricade scenes in Les Misérables.

## Early life

Charles Jeanne was born on May 15, 1800, in Paris, France. His father was a store clerk and he helped with his father's business. He attended school in Caen, but had to stop at age 14 due to financial and family issues. He was involved for a year in the French army and worked as a clerk.

## 1830 Rebellion

Charles Jeanne participated in the Trois Glorieuses, and was injured in action. Louis Phillippe gave him a medal for bravery.
# Charles Lloyd Barnhouse

Charles Lloyd Barnhouse (March 20, 1865 – November 18, 1929) was a 19th-century American music publisher. Barnhouse started a music publishing firm in 1886 that exists today as the C. L. Barnhouse Company, selling band music around the world.
Barnhouse was born and raised in Grafton, West Virginia where he became an expert cornet player and went upon the road with a theatrical company, later taking charge of a band at Mount Pleasant, Iowa, for three years and at Burlington, Iowa, for one year. In 1891 he moved to Oskaloosa, Iowa and took charge of the Iowa Brigade Band, of which he was leader for fifteen years.
In 1888, he had begun publishing music for bands, orchestras and miscellaneous musical organizations. He entered upon this work at Mount Pleasant and continued in the business in Burlington and Oskaloosa. He had a well-equipped printing plant and conducted a successful business. He also sold his own compositions and arrangements of music for bands and orchestras.
In 1886, he was married to Miss Josephine B. Scott, who was born in Pennsylvania in 1868 and was a daughter of J. H. and Mary E. (Hare) Scott. They had four children: Jamie M., Lloyd, Dorothy, and Irene.

## List of Works

* Red, White and "Blues" (1916)
* Favors for All (1927)
* Tripoli Temple March (1928)
* Our Flag Unfurled (1929)
* The Regulars (1929)
# Charlie Keetley

Frederick Charles "Charlie" "Wag" Keetley (10 March 1906 — 1979) was an English football centre forward born in Derby. He played for Leeds United, Bradford City and Reading.
Keetley was the youngest of eleven brothers and one sister. Several of his brothers played professionally including Arthur, Harry, Tom, Frank and Joe.
He began working at Rolls-Royce Foundry in Derby as a core maker. In 1926–27, he was playing for Alvaston and Boulton where he scored 80 goals.
Charlie was signed for Leeds United in June 1927 by Dick Ray, who had previous signed his 4 brothers for Doncaster Rovers. During the 1927–28 season Keetley scored 18 goals in just 16 games. He went on to be the club's top goal-scorer in three seasons—in 1928–29, 1930–31 and 1931–32. In his last full season at Elland Road he suffered from injury and lack of form, scoring 7 times in 16 appearances.
In October 1934 he moved to Bradford City where he played just 22 league games, scoring four goals, but also scored twice as City upset Aston Villa in a third round FA Cup tie on 12 January 1935.
At the end of the 1934–35 he moved to Reading and in 1936 to Stalybridge Celtic.
After finishing his football career he returned to work for Rolls-Royce before becoming licensee of the Sir Walter Scott on the Osmaston Road, Derby in about 1950, and later the New Inn at Chellaston, close to the pub his brother Tom ran.
# Christian Goethals

Christian Roger Xavier Marie Joseph Ghislain Goethals (4 August 1928 – 26 February 2003) was a racing driver from Belgium. Goethals competed as an amateur in sports car races, driving a Porsche Spyder during the 1950s. His best results were a second-place finish with his brother in the 1956 1500cc class event in Reims, and a win in the same class the following year at Forez. Goethals acquired a Cooper-Climax and entered it in the Formula Two class of the 1958 German Grand Prix, but retired from the race. He did not participate in another Formula One Grand Prix, and returned to sports cars, with notable finishes in 1960 of fifth in the Buenos Aires 1000 km and second in the GP de Spa. He retired from racing later in the season. He established a racing team called Écurie Éperon d'Or to participate in the 1958 German Grand Prix where he raced in a Cooper T43.
Goethals was the youngest child of René Goethals (1876-1928), a nobleman and mayor of Heule near Kortrijk in Belgium and of Jeanne Mols (1884-1968). He was married to Julie Opsomer.

## Biography in the Literature

* Steve SMALL, Grand Prix Who is Who, Travel Publishing, London 1999, ISBN 1-902007-46-8.
* Steve Small. The Guinness Complete Grand Prix Who's Who. p. 160. ISBN 0851127029.
* Humbert DE MARNIX DE SAINTE ALDEGONDE, État présent de la noblesse belge, Annuaire 2007, Brussel, 2007 (in French)
* Short biography in Grand Prix Insider

## Complete Formula One World Championship results

(key)
| Year | Entrant | Chassis | Engine | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | WDC | Points |
| 1958 | Écurie Éperon d'Or | Cooper T43 | Coventry Climax | ARG | MON | NED | 500 | BEL | FRA | GBR | GER Ret | POR | ITA | MOR | NC | 0 |
# Blendi Fevziu

Blendi Fevziu (born May 18, 1969, Tirana, Albania) is an Albanian journalist, writer and host of the TV talk show Opinion, which first went on air on August 31, 1997.
Fevziu graduated in literature and Albanian language at the University of Tirana in 1991. In 1989 he was part of the staff of the Student newspaper of the University. In December 1990 and February 1991 he was an active participant in the students' movement that brought the change of regime in Albania. On 5 January 1991 he was the co-founder of the Rilindja Demokratike (Democratic Rebirth) newspaper, the first free newspaper after almost 70 years of independent media blackout in Albania.

## Career in written press

* Chief editor of "Koha Jonë" newspaper 1992
* Chief editor of "Aleanca" newspaper 1993–1996
* Co-chief editor of "Poli i Qendrës" newspaper 1996
* Editor of political news "Independent" newspaper 1997
* Editor of Klan Magazine 1996–2001
* Editor of "Korrieri" newspaper 2001–2005
* Editor of "Koha Jonë" newspaper 2007–2008

## Career in TV

On August 31, 1997, Fevziu started a political show on TVSH named E Diela Debat, a weekly political debate that airs every sunday. On September 26, 1998, the show was moved to Klan Television and, from 1999, the show aired on Thursdays instead of Sundays.
In 2001, it was renamed "Opinion", and from October 2006 it was broadcast twice a week. Since 2008, "Opinion" has aired four times a week with Mondays to Wednesdays at 22:30 (10:30 PM), and Fridays at 21:00 (9 PM).
# Chris Webber (basketball, born 1972)

Chris Webber (born December 4, 1972) is a Canadian former professional basketball player, most notable for his career in the British Basketball League.

## Career

Born in Owen Sound, Ontario, Webber's career began in 1992–93 with the Carleton University Ravens, where he won the Rookie of the Year title for Ontario-East. Webber transferred to his home-town University of Western Ontario for second year. As a student of the University of Western Ontario, Webber played with the senior basketball team, the Western Ontario Mustangs, where he was named to the OUAA Conference 1st Team's for 1995–96 and 1996–97.
In October 1998, the 6'9 forward signed a professional contract with British Basketball League (BBL) outfit Newcastle Eagles, however he was released after just one month at the club and subsequently joined in November Plymouth Raiders to replace Daniel Okonkwo who was in Greece representing Nigeria in the 1998 FIBA World Championship.
Following Okonkwo's return, Webber was then signed by BBL franchise Leicester City Riders, making his debut on December 12 at home to London Towers. Having moved on again to Birmingham Bullets and then returned to Newcastle the following season, Webber eventually settled with Edinburgh Rocks in 2000, before ending a two-year spell to play for Gymnastikos S. Larissas in Greece. After just one season, Webber returned to the BBL and to former club Leicester, where he saw out one final season.
Chris Webber is currently a gym instructor at Newton's Grove School in Mississauga, Ontario.

## Career history

* 2003–2004 Leicester Riders
* 2002–2003 Gymnastikos S. Larissas
* 2000–2002 Edinburgh Rocks
* 1999–2000 Newcastle Eagles
* 1998–1999 Birmingham Bullets
* 1998 Leicester City Riders
* 1998 Plymouth Raiders
* 1998 Newcastle Eagles
* Western Ontario Mustangs
# Enes Alić

Enes Alić (born 3 September 1999) is a Bosnian football defender who plays for the Croatian club Varaždin.

## Career

On 30 January 2023, Alić moved to Kisvárda in Hungary.
Alić was signed by NK Varaždin in Croatia on August 31, 2024.
# Tulip Cemetery

Tulip Cemetery is a historic cemetery in Tulip, a small hamlet in rural Dallas County, Arkansas. It is located off Arkansas Highway 9, just behind the Tulip Methodist Church, occupying a high spot in the area. Tulip was one of the first settlements in Dallas County; the cemetery's oldest documented grave dates to 1847. It also includes the graves of six Confederate Army soldiers.
The cemetery was listed on the National Register of Historic Places in 1983.
* Headstones at cemetery
* Tulip Cemetery Church
* Field of headstones
* Headstone from 1857
* Headstone
* Main gates
# Kamieniec Ząbkowicki

Kamieniec Ząbkowicki (German: Kamenz N.S.) is a town in Ząbkowice Śląskie County, Lower Silesian Voivodeship, in south-western Poland. It is the seat of the administrative district (gmina) called Gmina Kamieniec Ząbkowicki. The town is an important railroad junction, located on the main line which links Wrocław with Kłodzko and Prague. In Kamieniec, this route crosses with the west-east connection from Jaworzyna Śląska to Kędzierzyn-Koźle.
It lies approximately 8 km (5 mi) south-east of Ząbkowice Śląskie, and 68 km (42 mi) south of the regional capital Wrocław. The village has a population of 4,200.
The architectural and landscape ensemble, containing the local landmarks, the abbey and palace, is a Historic Monument of Poland.

## History

The name of the town comes from the Polish word kamień, which means "stone". It was mentioned in the oldest Polish chronicle Gesta principum Polonorum from the early 12th century. The place is known for the former Kamieniec Abbey, established in 1209 as an Augustinian college by Bishop Wawrzyniec of Wrocław at the site of a former castle of Bretislaus II of Bohemia. It was then part of the Silesian Duchy of Piast-ruled Poland. In 1247 it became a filial monastery of the Cistercian Lubiąż Abbey.
King Frederick II of Prussia hid here from Habsburg troops on 27 February 1741 during the First Silesian War. Following the war, the village fell under Prussian suzerainty. Secularized in 1810 by order of King Frederick William III of Prussia, the estates of Kamieniec (then under the Germanized name Kamenz) were acquired by Wilhelmine of Prussia, wife of King William I of the Netherlands. Between 1838 and 1873 their daughter Princess Marianne of the Netherlands and her husband Prince Albert of Prussia had a new palace built in a Neogothic style according to the plans of Karl Friedrich Schinkel.
In 1871 the settlement became part of Germany, and during World War II Nazi Germany carried out murders of mentally ill children by involuntary euthanasia in the local monastery building (see: Aktion T4). In the final stages of the war, the palace was plundered and set on fire by the occupying Soviet Red Army.
Following the war, in 1945, the village became again part of Poland, and the adjective Ząbkowicki (after the nearby town and county seat of Ząbkowice Śląskie) was added to the name to distinguish it from other settlements of the same name, which are very common throughout Poland. The palace was restored in 1995.
In July 2020, Kamieniec Ząbkowicki received town rights with effect from 2021.

## Sports

The local football team is Zamek Kamieniec Ząbkowicki. It competes in the lower leagues.

## Gallery

* Gothic-Baroque Church of the Assumption
* Abbey building, now the Polish state archive
* Palace park
* Monument of Pope John Paul II

## Surroundings

* Gola Dzierżoniowska Castle
* Medieval town of Niemcza
* Cistercian monastery at Henryków
* Wojsławice Arboretum
# Efrem Smith

Efrem Smith is an American pastor who is the co-pastor of Midtown Church and co-owner of Influential LLC. Smith has spoken on the subjects of leadership, multi-ethnic issues and development of the Christian community. Smith advocates for urban ministry and reaching out to the marginalized.

## Education

Smith graduated from Saint John's University, Luther Theological Seminary, and a DMin from Fuller Theological Seminary.

## Career

Smith has served as the founding pastor of The Sanctuary Covenant Church and is involved in community foundations as he was formerly the President of the Sanctuary Community Development Corporation in Minneapolis, Minnesota and the superintendent of the Pacific Southwest Conference of the Evangelical Covenant Church. In 1993 Smith was ordained by the National Baptist Convention. Currently, Smith serves as the President and CEO of World Impact as his full-time ministry and is an itinerant speaker for Forge: Kingdom Building Ministries.

## Speaking engagements

As a motivational speaker with Kingdom Building Ministries, Smith has been a keynote speaker for Together LA, the Urban Youth Worker's Institute Convention, Flavor Fest, Willow Creek Association Leadership Summit and Exponential Conferences.

## Books

* Raising Up Young Heroes ISBN 978-0-8308-3209-5
* The Hip-Hop Church ISBN 978-0-8308-3329-0
* Jump ISBN 978-1-4347-6457-7
* The Post-Black and Post-White Church ISBN 978-1-118-03658-7
* Hip-Hop as Culture

## Personal life

Smith lives in the Bay area with his wife Donecia and two daughters.
# Limited Edition Sportswear

Limited Edition Sportswear, generally styled as LE, is an Australian sportswear clothing company which was established in 2008 in the city of Brisbane in Queensland. It is owned by its founder, Kris Pillay. Its slogan is "Cultured by Choice Branded with Innovation". The company has a network of suppliers in UK, Australia, Pacific Islands including Samoa, Fiji, New Zealand and Papua New Guinea.

## Sponsorships
### Rugby Union

* Samoa (since 2016 – Until 2020)

### Rugby League

* Fiji (2013-2017)
* Wellington Rugby League
* South Island Scorpions
* Northern Swords
# Howlin' for You

"Howlin' for You" is a song by The Black Keys from the band's sixth album, Brothers.

## Music video

An official video was released for the song. The music video—a parody of a sexploitation film trailer—was directed by Chris Marrs Piliero, starring Tricia Helfer, Diora Baird, Sean Patrick Flanery, Christian Serratos, Corbin Bernsen, Todd Bridges, and Shaun White, as well as Dan Auerbach and Patrick Carney of the band in the role of "Las Teclas de Negro" (translated from Spanish as "The Keys of Black").
A behind-the-scenes interview with Piliero, filmed during production of the video, has tongue-in-cheek commentary and brief clips of the cast members describing their characters.
The video was one of five nominees for the 2011 MTV Video Music Award for Best Rock Video.

## Critical reception

"Howlin' for You" is widely regarded as one of the band's best songs. In 2012, Complex ranked the song number ten on their list of the 15 greatest Black Keys songs, and in 2021, American Songwriter ranked the song number four on their list of the 10 greatest Black Keys songs.

## Appearances in other media

"Howlin' for You" is featured on the soundtrack of the EA Sports video games, NHL 11, NHL Slapshot and Need for Speed Payback, as well as NBA 2K14. The song has been featured in TV commercials and films for The Dilemma, Limitless, The Guilt Trip, Moneyball, Dark Shadows, Citizen Gangster, Deadpool, Venom: Let There Be Carnage, and The Bad Guys. It is used as the theme song to the Australian police drama television series, Cops L.A.C., and has been featured in American television series, CSI: Miami, Detroit 1-8-7, Entourage, Once Upon a Time, Prime Suspect, Chuck, Necessary Roughness, Suits, Lucifer, Secret Diary of a Call Girl and The Chicago Code. MLB's Toronto Blue Jays used it as their home run song (except for José Bautista), and the NHL's Arizona Coyotes used it as their goal song.

## Certifications

| Region | Certification | Certified units/sales |
| Canada (Music Canada) | Gold | 40,000$^*$ |
| Italy (FIMI) | Gold | 25,000$^‡$ |
| United Kingdom (BPI) | Silver | 200,000$^‡$ |
| United States (RIAA) | Platinum | 1,000,000$^‡$ |
| $^*$ Sales figures based on certification alone. $^‡$ Sales+streaming figures based on certification alone. |||
# Bielsa

Bielsa is a municipality located in the province of Huesca, Aragon, Spain. According to the 2004 census (INE), the municipality has a population of 463 inhabitants.
The Bielsa tunnel under the Pyrenean mountains connects Bielsa and Aragnouet in France. It was first opened in 1970. In 2002, the French government unilaterally decided to close the tunnel to heavy-goods vehicles over 3.5 tonnes, a decision which caused serious concern in Aragon.
# Real History

Real History, formerly known as Fox History, The History Channel and simply History is a television channel in Australia and New Zealand, that broadcasts non-fictional programs regarding historical events and persons, as well as various metaphysical, pseudoscientific, and paranormal phenomena—often with observations and explanations by noted historians, scholars, authors, esotericists, astrologers, and Biblical scholars as well as reenactments and interviews with witnesses, and/or families of witnesses.
The channel is operated by Foxtel Networks. From 1998 to 2024, the programming and name of the channel was licensed to Foxtel by A&E Networks.
It started out as Fox History on 1 August 1996, and changed its name to The History Channel in November 1998. The channel used to share its frequency with Fox Kids until December 2000, when it got its own 24-hour channel.
On 3 November 2014, History launched a high-definition feed.
On 1 August 2024, the channel rebranded as Real History.
# Khalil Chemmam

Khalil Chemmam (Arabic: خليل شمام; born 24 July 1987) is a Tunisian retired professional footballer who played as a left back and centre back for Espérance de Tunis and Vitória de Guimarães.

## Club career

Born in Tunis, Chemmam has played club football for Espérance Tunis, Vitória de Guimarães B and Vitória de Guimarães. He participated at the FIFA Club World Cup in 2011, 2018 and 2019.

## International career

Chemmam earned 26 caps for the Tunisia national team between 2008 and 2013. In May 2018 he was named in Tunisia’s preliminary 29-man squad for the 2018 World Cup in Russia.
# Lan Jingxuan

Lan Jingxuan (Chinese: 兰菁轩; born 16 September 1999) is a Chinese footballer who currently plays for China League Two club Quanzhou Yassin.

## Club career

Lan Jingxuan was promoted to the senior team of Tianjin TEDA within the 2019 Chinese Super League season and he would make his debut on 30 April 2019 in a Chinese FA Cup game against Zibo Cuju F.C. in a 4-1 victory.

## Career statistics

    As of 31 December 2020.
| Club | Season | League | League | League | Cup | Cup | Continental | Continental | Other | Other | Total | Total |
| Club | Season | Division | Apps | Goals | Apps | Goals | Apps | Goals | Apps | Goals | Apps | Goals |
| Tianjin TEDA | 2019 | Chinese Super League | 0 | 0 | 1 | 0 | – | – | – | – | 1 | 0 |
| Tianjin TEDA | 2020 | Chinese Super League | 1 | 0 | 1 | 0 | – | – | – | – | 2 | 0 |
| Tianjin TEDA | Total | Total | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 0 |
| Career total | Career total | Career total | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 0 |
# Leanid Harai

Leanid Paulavich Harai (Belarusian: Леанід Паўлавіч Гарай; Russian: Леонид Павлович Гарай; 13 January 1937 – 5 August 2019) was a Soviet footballer and manager.

## Playing career

Harai's father was killed in World War II when he was age 4. After the war, Harai began playing football at school in Brest and then Minsk. He would join FC Spartak Minsk's senior side where he began playing in the second-tier Soviet Class B league. In 1960, Harai joined FC Urozhay Minsk. The following season he joined FC Belarus Minsk, a club composed of several former Spartak and Urozhay players, which competed in the 1961 Soviet Top League. Harai finished his playing career with spells at FC SKA Minsk and FC Spartak Brest.

## Coaching career

After he retired from playing, Harai embarked on a managerial career. He led FC Spartak Brest to its highest-ever level in Soviet football, reaching the 1969 Soviet Class A Second Group.
# Leonid Tkachenko (footballer)

Leonid Ivanovich Tkachenko (Russian: Леонид Иванович Ткаченко; 1 October 1953 – 4 January 2024) was a Ukrainian-Russian football coach and a Soviet player.

## Career

Tkachenko spent most of his career playing for Metalist Kharkiv, leading the club to promotion from the Soviet First League to the Soviet Top League in 1981.
Together with Mykola Pavlov he served as an interim coach for Ukraine national team when it contested Belarus in a friendly. He and Pavlov were assistant coaches to Viktor Prokopenko before then. Sometime in 2000, he relocated to the Russian Federation and obtained Russian citizenship.

## Death

Tkachenko died on 4 January 2024, at the age of 70.

## Honours

* Russian Second Division Zone West best manager: 2005.