# List of National Wildlife Refuges

As of 2022, there are 588 National Wildlife Refuges in the United States, with the addition of the Green River National Wildlife Refuge. Refuges that have boundaries in multiple states are listed only in the state where the main visitor entrance is located. The newest refuge replaces the Cherry Valley National Wildlife Refuge in Pennsylvania. Refuges are listed regardless of whether or not they are open to the public, which many are not.
The United States is divided into eight regions for administrative purposes:
| Pacific Region (Region 1) American Samoa Guam Hawaiʻi Idaho Northern Mariana Islands Oregon U.S. Minor Outlying Islands (1 and 4) Washington | Southwest Region (Region 2) Arizona New Mexico Oklahoma Texas | Midwest Region (Region 3) Illinois Indiana Iowa Michigan Missouri Minnesota Ohio Wisconsin | Southeast Region (Region 4) Alabama Arkansas Florida Georgia Kentucky Louisiana Mississippi North Carolina Puerto Rico South Carolina Tennessee U.S. Minor Outlying Islands (1 and 4) U.S. Virgin Islands |
| Northeast Region (Region 5) Connecticut Delaware District of Columbia Maine Maryland Massachusetts New Hampshire New Jersey New York Pennsylvania Rhode Island Vermont Virginia West Virginia | Mountain-Prairie Region (Region 6) Colorado Kansas Montana North Dakota Nebraska South Dakota Utah Wyoming | Alaska Region (Region 7) Alaska | Pacific Southwest Region (Region 8) California Nevada |
| Refuge Name | Location | State | Date Established | Area |
| Bon Secour National Wildlife Refuge | Baldwin County | AL | 1980 | 6,816 acres (27.58 km$^2$) |
| Cahaba River National Wildlife Refuge | Bibb County | AL | September 25, 2002 | 2,997 acres (12.13 km$^2$) |
| Choctaw National Wildlife Refuge | Choctaw County | AL | 1964 | 4,218 acres (17.07 km$^2$) |
| Eufaula National Wildlife Refuge | Barbour County, Alabama Stewart County, Georgia | AL | 1964 | 11,184 acres (45.26 km$^2$) |
| Fern Cave National Wildlife Refuge | Jackson County | AL | 1981 | 199 acres (0.81 km$^2$) |
| Key Cave National Wildlife Refuge | Lauderdale County | AL | January 3, 1997 | 1,060 acres (4.3 km$^2$) |
| Mountain Longleaf National Wildlife Refuge | Calhoun County | AL | May 29, 2003 | 9,016 acres (36.49 km$^2$) |
| Sauta Cave National Wildlife Refuge | Jackson County | AL | 1978 | 264 acres (1.07 km$^2$) |
| Watercress Darter National Wildlife Refuge | Jefferson County | AL | 1980 | 24 acres (0.097 km$^2$) |
| Wheeler National Wildlife Refuge | Morgan County, Limestone County, and Madison County | AL | 1938 | 35,000 acres (140 km$^2$) |
| Alaska Maritime National Wildlife Refuge | Alaska | AK | December 2, 1980 | 3,421,420 acres (13,846.0 km$^2$) |
| Alaska Peninsula National Wildlife Refuge | Alaska | AK | December 2, 1980 | 3,574,259 acres (14,464.51 km$^2$) |
| Arctic National Wildlife Refuge | Alaska | AK | December 6, 1960 | 19,287,042 acres (78,051.89 km$^2$) |
| Becharof National Wildlife Refuge | Alaska | AK | December 2, 1980 | 1,200,419.52 acres (4,857.9254 km$^2$) |
| Innoko National Wildlife Refuge | Alaska | AK | December 2, 1980 | 3,850,800.94 acres (15,583.6385 km$^2$) |
| Izembek National Wildlife Refuge | Alaska | AK | December 2, 1980 | 315,000 acres (1,270 km$^2$) |
| Kanuti National Wildlife Refuge | Alaska | AK | December 2, 1980 | 1,637,000 acres (6,620 km$^2$) |
| Kenai National Wildlife Refuge | Alaska | AK | December 2, 1980 | 1,912,470.47 acres (7,739.4934 km$^2$) |
| Kodiak National Wildlife Refuge | Alaska | AK | August 19, 1941 | 1,880,891.52 acres (7,611.6979 km$^2$) |
| Koyukuk National Wildlife Refuge | Alaska | AK | 1980 | 3,550,159.96 acres (14,366.9876 km$^2$) |
| Nowitna National Wildlife Refuge | Alaska | AK | 1980 | 1,560,239.99 acres (6,314.0672 km$^2$) |
| Selawik National Wildlife Refuge | Alaska | AK | December 2, 1980 | 2,150,561.94 acres (8,703.0154 km$^2$) |
| Tetlin National Wildlife Refuge | Alaska | AK | December 2, 1980 | 700,286.95 acres (2,833.9607 km$^2$) |
| Togiak National Wildlife Refuge | Alaska | AK | December 2, 1980 | 4,104,246.80 acres (16,609.2975 km$^2$) |
| Yukon Delta National Wildlife Refuge | Alaska | AK | December 2, 1980 | 19,226,606.25 acres (77,807.3150 km$^2$) |
| Yukon Flats National Wildlife Refuge | Alaska | AK | December 2, 1980 | 8,636,951.06 acres (34,952.5009 km$^2$) |
| Rose Atoll National Wildlife Refuge | Rose Atoll | AS | 1973 | 39,066 acres (158.09 km$^2$) |
| Bill Williams River National Wildlife Refuge | La Paz and Mojave Counties | AZ | 1993 | 6,105 acres (24.71 km$^2$) |
| Buenos Aires National Wildlife Refuge | Pima County | AZ | 1985 | 117,107 acres (473.92 km$^2$) |
| Cabeza Prieta National Wildlife Refuge | Yuma County | AZ | 1939 | 860,010 acres (3,480.3 km$^2$) |
| Cibola National Wildlife Refuge | LaPaz County, Arizona Imperial County, California | AZ | 1964 | 16,627 acres (67.29 km$^2$) |
| Havasu National Wildlife Refuge | Mojave County, Arizona San Bernardino County, California | AZ | 1941 | 37,515 acres (151.82 km$^2$) |
| Imperial National Wildlife Refuge | LaPaz and Yuma Counties, Arizona Imperial County, California | AZ | 1941 | 25,768 acres (104.28 km$^2$) |
| Kofa National Wildlife Refuge | Yuma and LaPaz Counties | AZ | 1939 | 665,400 acres (2,693 km$^2$) |
| Leslie Canyon National Wildlife Refuge | Cochise County | AZ | 1988 | 2,770 acres (11.2 km$^2$) |
| San Bernardino National Wildlife Refuge | Cochise County | AZ | 1982 | 2,309 acres (9.34 km$^2$) |
| Bald Knob National Wildlife Refuge | White County | AR | 1993 | 15,022 acres (60.79 km$^2$) |
| Big Lake National Wildlife Refuge | Mississippi County | AR | 1915 | 11,038 acres (44.67 km$^2$) |
| Cache River National Wildlife Refuge | Woodruff County and several others | AR | 1986 | 67,500 acres (273 km$^2$) |
| Felsenthal National Wildlife Refuge | Union, Ashley, and Bradley Counties | AR | 1970 | 64,902 acres (262.65 km$^2$) |
| Holla Bend National Wildlife Refuge | Pope and Yell Counties | AR | 1957 | 7,057 acres (28.56 km$^2$) |
| Logan Cave National Wildlife Refuge | Benton County | AR | March 14, 1989 | 123 acres (0.50 km$^2$) |
| Overflow National Wildlife Refuge | Ashley County | AR | 1980 | 13,973 acres (56.55 km$^2$) |
| Pond Creek National Wildlife Refuge | Sevier County | AR | 1994 | 27,300 acres (110 km$^2$) |
| Wapanocca National Wildlife Refuge | Crittenden County | AR | January 1961 | 5,484 acres (22.19 km$^2$) |
| White River National Wildlife Refuge | Monroe County and several others | AR | 1935 | 160,756 acres (650.56 km$^2$) |
| Antioch Dunes National Wildlife Refuge | Contra Costa County | CA | 1980 | 55 acres (0.22 km$^2$) |
| Bitter Creek National Wildlife Refuge | Kern County | CA | 1985 | 14,097 acres (57.05 km$^2$) |
| Blue Ridge National Wildlife Refuge | Tulare County | CA | 1982 | 897 acres (3.63 km$^2$) |
| Butte Sink Wildlife Management Area | Butte/Colusa/Sutter Counties | CA | 1980 | 11,044 acres (44.69 km$^2$) |
| Castle Rock National Wildlife Refuge | Del Norte County | CA | 1979 | 14 acres (0.057 km$^2$) |
| Clear Lake National Wildlife Refuge | Modoc County | CA | 1911 | 46,460 acres (188.0 km$^2$) |
| Coachella Valley National Wildlife Refuge | Riverside County | CA | 1985 | 3,709 acres (15.01 km$^2$) |
| Colusa National Wildlife Refuge | Colusa County | CA | 1945 | 4,507 acres (18.24 km$^2$) |
| Delevan National Wildlife Refuge | Colusa County | CA | 1962 | 5,797 acres (23.46 km$^2$) |
| Don Edwards San Francisco Bay National Wildlife Refuge | Alameda/Santa Clara/San Mateo Counties | CA | 1974 | 30,000 acres (120 km$^2$) |
| Ellicott Slough National Wildlife Refuge | Santa Cruz County | CA | 1975 | 168 acres (0.68 km$^2$) |
| Farallon National Wildlife Refuge | Farallon Islands, near San Francisco | CA | 1969 | 41.9 acres (0.170 km$^2$) |
| Grasslands Wildlife Management Area | Merced County | CA | 1979 | 70,000 acres (280 km$^2$) |
| Guadalupe-Nipomo Dunes National Wildlife Refuge | Santa Barbara/San Luis Obispo Counties | CA | 2000 | 2,553 acres (10.33 km$^2$) |
| Hopper Mountain National Wildlife Refuge | Ventura County | CA | 1974 | 2,471 acres (10.00 km$^2$) |
| Humboldt Bay National Wildlife Refuge *Lanphere Dunes | Humboldt County | CA | 1971 | 4,000 acres (16 km$^2$) |
| Kern National Wildlife Refuge | Kern County | CA | 1960 | 1,249 acres (5.05 km$^2$) |
| Lower Klamath National Wildlife Refuge | Siskiyou County also in Klamath County, Oregon | CA | 1908 | 50,913 acres (206.04 km$^2$) |
| Marin Islands National Wildlife Refuge | Marin County | CA | 1992 | - |
| Merced National Wildlife Refuge | Merced County | CA | 1951 | 10,262 acres (41.53 km$^2$) |
| Modoc National Wildlife Refuge | Modoc County | CA | 1961 | 7,000 acres (28 km$^2$) |
| North Central Valley Wildlife Management Area | Sacramento Valley | CA | 1991 | 46,460 acres (188.0 km$^2$) |
| Pixley National Wildlife Refuge | Tulare County | CA | 1959 | 6,939 acres (28.08 km$^2$) |
| Sacramento National Wildlife Refuge | Colusa/Glenn Counties | CA | 1937 | 10,819 acres (43.78 km$^2$) |
| Sacramento River National Wildlife Refuge | Butte/Glenn/Tehama Counties | CA | 1989 | 10,146 acres (41.06 km$^2$) |
| Salinas River National Wildlife Refuge | Monterey County | CA | 1974 | 316 acres (1.28 km$^2$) |
| San Diego Bay National Wildlife Refuge | San Diego County | CA | 1999 | 2,620 acres (10.6 km$^2$) |
| San Diego National Wildlife Refuge | San Diego County | CA | - | 11,152 acres (45.13 km$^2$) |
| San Joaquin River National Wildlife Refuge | San Joaquin/Stanislaus Counties | CA | 1987 | 7,000 acres (28 km$^2$) |
| San Luis National Wildlife Refuge | Merced County | CA | 1966 | 26,600 acres (108 km$^2$) |
| San Pablo Bay National Wildlife Refuge | Napa/Solano/Sonoma Counties | CA | 1970 | 13,190 acres (53.4 km$^2$) |
| Seal Beach National Wildlife Refuge | Orange County | CA | 1972 | 911 acres (3.69 km$^2$) |
| Sonny Bono Salton Sea National Wildlife Refuge | Imperial County | CA | 1930 | 2,200 acres (8.9 km$^2$) |
| Stone Lakes National Wildlife Refuge | Sacramento County | CA | 1994 | 17,641 acres (71.39 km$^2$) |
| Sutter National Wildlife Refuge | Sutter County | CA | 1945 | 2,591 acres (10.49 km$^2$) |
| Tijuana Slough National Wildlife Refuge | San Diego County | CA | 1980 | 2,521 acres (10.20 km$^2$) |
| Tule Lake National Wildlife Refuge | Modoc/Siskiyou Counties | CA | 1928 | 39,116 acres (158.30 km$^2$) |
| Willow Creek-Lurline Wildlife Management Area | Colusa/Glenn Counties | CA | 1985 | 5,795 acres (23.45 km$^2$) |
| Alamosa National Wildlife Refuge | Alamosa, Conejos and Costilla Counties | CO | 1993 | 11,169 acres (45.20 km$^2$) |
| Arapaho National Wildlife Refuge | Jackson County | CO | 1967 | 23,464 acres (94.96 km$^2$) |
| Baca National Wildlife Refuge | Saguache and Alamosa Counties | CO | 2003 | 78,697 acres (318.48 km$^2$) |
| Browns Park National Wildlife Refuge | Moffat County | CO | 1965 | 12,150 acres (49.2 km$^2$) |
| Monte Vista National Wildlife Refuge | Alamosa and Rio Grande Counties | CO | 1953 | 14,800 acres (60 km$^2$) |
| Rocky Flats National Wildlife Refuge | Jefferson County | CO | July 12, 2007 | 3,953 acres (16.00 km$^2$) |
| Rocky Mountain Arsenal National Wildlife Refuge | Adams County | CO | 1992 | 15,988 acres (64.70 km$^2$) |
| Two Ponds National Wildlife Refuge | Jefferson County | CO | 1992 | 72.2 acres (0.292 km$^2$) |
| Stewart B. McKinney National Wildlife Refuge | Connecticut | CT | 1972 | 950 acres (3.8 km$^2$) |
| Bombay Hook National Wildlife Refuge | Kent County | DE | 1937 | 16,251 acres (65.77 km$^2$) |
| Prime Hook National Wildlife Refuge | Sussex County | DE | 1963 | 10,144 acres (41.05 km$^2$) |
| Archie Carr National Wildlife Refuge | Brevard County Indian River County | FL | 1991 | 900 acres (3.6 km$^2$) |
| Arthur R. Marshall Loxahatchee National Wildlife Refuge | Palm Beach County | FL | 1951 | 147,392 acres (596.47 km$^2$) |
| Caloosahatchee National Wildlife Refuge | Lee County | FL | 1921 | 40 acres (0.16 km$^2$) |
| Cedar Keys National Wildlife Refuge | Levy County | FL | 1929 | 891 acres (3.61 km$^2$) |
| Chassahowitzka National Wildlife Refuge | Citrus County Hernando County | FL | 1941 | 31,000 acres (130 km$^2$) |
| Crocodile Lake National Wildlife Refuge | Monroe County | FL | 1980 | 6,686 acres (27.06 km$^2$) |
| Crystal River National Wildlife Refuge | Citrus County | FL | 1983 | 80 acres (0.32 km$^2$) |
| Egmont Key National Wildlife Refuge | Hillsborough County | FL | 1974 | 450 acres (1.8 km$^2$) |
| Everglades Headwaters National Wildlife Refuge and Conservation Area | Hardee County Highlands County Polk County | FL | 1991 | 900 acres (3.6 km$^2$) |
| Florida Panther National Wildlife Refuge | Collier County | FL | 1989 | 26,400 acres (107 km$^2$) |
| Great White Heron National Wildlife Refuge | Monroe County | FL | 1938 | 130,187 acres (526.85 km$^2$) |
| Hobe Sound National Wildlife Refuge | Martin County | FL | 1969 | 1,035 acres (4.19 km$^2$) |
| Island Bay National Wildlife Refuge | Charlotte County | FL | 1908 | 20 acres (0.081 km$^2$) |
| J.N. 'Ding' Darling National Wildlife Refuge | Lee County | FL | 1976 | 5,200 acres (21 km$^2$) |
| Key Deer National Wildlife Refuge | Monroe County | FL | 1957 | 8,542 acres (34.57 km$^2$) |
| Key West National Wildlife Refuge | Monroe County | FL | 1908 | 189,497 acres (766.87 km$^2$) |
| Lake Wales Ridge National Wildlife Refuge | Highlands County Polk County | FL | 1990 | 1,194 acres (4.83 km$^2$) |
| Lake Woodruff National Wildlife Refuge | Lake County Volusia County | FL | 1964 | 21,574 acres (87.31 km$^2$) |
| Lower Suwannee National Wildlife Refuge | Dixie County Levy County | FL | 1979 | 53,000 acres (210 km$^2$) |
| Matlacha Pass National Wildlife Refuge | Lee County | FL | 1908 | 538 acres (2.18 km$^2$) |
| Merritt Island National Wildlife Refuge | Brevard County | FL | 1963 | 140,000 acres (570 km$^2$) |
| Passage Key National Wildlife Refuge | Manatee County | FL | 1905 | 64 acres (0.26 km$^2$) |
| Pelican Island National Wildlife Refuge | Indian River County | FL | 1903-First National Wildlife Refuge in U.S. | 5,376 acres (21.76 km$^2$) |
| Pine Island National Wildlife Refuge | Lee County | FL | 1908 | 601 acres (2.43 km$^2$) |
| Pinellas National Wildlife Refuge | Pinellas County | FL | 1951 | 394 acres (1.59 km$^2$) |
| St. Johns National Wildlife Refuge | Brevard County | FL | 1971 | 6,255 acres (25.31 km$^2$) |
| St. Marks National Wildlife Refuge | Jefferson County Taylor County Wakulla County | FL | 1931 | 68,000 acres (280 km$^2$) |
| St. Vincent National Wildlife Refuge | Franklin County Gulf County | FL | 1968 | 12,490 acres (50.5 km$^2$) |
| Ten Thousand Islands National Wildlife Refuge | Collier County Monroe County | FL | 1996 | 35,000 acres (140 km$^2$) |
| Banks Lake National Wildlife Refuge | Lanier County | GA | 1985 | 4,049 acres (16.39 km$^2$) |
| Blackbeard Island National Wildlife Refuge (Part of the Savannah Coastal Refuges Complex) | McIntosh County | GA | 1924 | 5,618 acres (22.74 km$^2$) |
| Bond Swamp National Wildlife Refuge | Bibb County Twiggs County | GA | 1989 | 6,500 acres (26 km$^2$) |
| Harris Neck National Wildlife Refuge (Part of the Savannah Coastal Refuges Complex) | McIntosh County | GA | 1962 | 2,762 acres (11.18 km$^2$) |
| Okefenokee National Wildlife Refuge | Primarily in Charlton and Ware Counties, Georgia and Baker County, Florida | GA | 1937 | 402,000 acres (1,630 km$^2$) |
| Piedmont National Wildlife Refuge | Jasper County Jones County | GA | 1939 | 35,000 acres (140 km$^2$) |
| Savannah National Wildlife Refuge* | Chatham and Effingham Counties, Georgia and Jasper County, South Carolina | GA | 1927 | 29,175 acres (118.07 km$^2$) |
| Wassaw National Wildlife Refuge (Part of the Savannah Coastal Refuges Complex) | Chatham County | GA | 1969 | 10,053 acres (40.68 km$^2$) |
| Wolf Island National Wildlife Refuge (Part of the Savannah Coastal Refuges Complex) | McIntosh County | GA | 1930 | 5,126 acres (20.74 km$^2$) |
| Guam National Wildlife Refuge | Guam | GU | 1993 | 1,203 acres (4.87 km$^2$) |
| Hakalau Forest National Wildlife Refuge | Island of Hawaiʻi | HI | 1985 | 38,047 acres (153.97 km$^2$) |
| Hanalei National Wildlife Refuge | Island of Kauaʻi | HI | 1972 | 917 acres (3.71 km$^2$) |
| Hawaiian Islands National Wildlife Refuge | Hawaiʻi | HI | 1909 | 245,000 acres (990 km$^2$) |
| Hulē‘ia National Wildlife Refuge | Island of Kauaʻi | HI | 1973 | 241 acres (0.98 km$^2$) |
| James Campbell National Wildlife Refuge | Island of Oʻahu | HI | 1976 | 1,100 acres (4.5 km$^2$) |
| Kakahai‘a National Wildlife Refuge | Island of Molokaʻi | HI | 1977 | 44 acres (0.18 km$^2$) |
| Kealia Pond National Wildlife Refuge | Island of Maui | HI | 1992 | 700 acres (2.8 km$^2$) |
| Kilauea Point National Wildlife Refuge | Island of Kauaʻi | HI | 1985 | 203 acres (0.82 km$^2$) |
| Oahu Forest National Wildlife Refuge | Island of Oʻahu | HI | 2000 | 4,525 acres (18.31 km$^2$) |
| Pearl Harbor National Wildlife Refuge | Island of Oʻahu | HI | 1972 | 63 acres (0.25 km$^2$) |
| Bear Lake National Wildlife Refuge (Part of the Southeast Idaho National Wildlife Refuge Complex) | Bear Lake County | ID | 1968 | 19,000 acres (77 km$^2$) |
| Camas National Wildlife Refuge (Part of the Southeast Idaho National Wildlife Refuge Complex) | Jefferson County | ID | 1937 | 10,578 acres (42.81 km$^2$) |
| Deer Flat National Wildlife Refuge | Canyon County Owyhee County Payette County Washington County Malheur County, Oregon Baker County, Oregon | ID | 1909 | 10,548 acres (42.69 km$^2$) |
| Grays Lake National Wildlife Refuge (Part of the Southeast Idaho National Wildlife Refuge Complex) | Bonneville County Caribou County | ID | 1965 | 19,400 acres (79 km$^2$) |
| Kootenai National Wildlife Refuge | Boundary County | ID | 1964 | 2,774 acres (11.23 km$^2$) |
| Minidoka National Wildlife Refuge (Part of the Southeast Idaho National Wildlife Refuge Complex) | Blaine County Cassia County Minidoka County Power County | ID | 1909 | 20,751 acres (83.98 km$^2$) |
| Oxford Slough Waterfowl Production Area | Bannock County Franklin County | ID | 1985 | 1,878 acres (7.60 km$^2$) |
| Chautauqua National Wildlife Refuge | Mason County | IL | 1936 | 4,388 acres (17.76 km$^2$) |
| Crab Orchard National Wildlife Refuge | Jackson County Union County Williamson County | IL | 1947 | 43,890 acres (177.6 km$^2$) |
| Cypress Creek National Wildlife Refuge | Alexander County Johnson County Pulaski County Union County | IL | 1990 | 16,000 acres (65 km$^2$) |
| Emiquon National Wildlife Refuge | Fulton County | IL | 1993 | 11,122 acres (45.01 km$^2$) |
| Hackmatack National Wildlife Refuge | McHenry County Walworth County, Wisconsin | IL | 2012 | 11,200 acres (45 km$^2$) |
| Kankakee National Wildlife Refuge and Conservation Area | Iroquois County | IL | 2016 | 66 acres (0.27 km$^2$) |
| Meredosia National Wildlife Refuge | Cass County Morgan County | IL | 1973 | 5,255 acres (21.27 km$^2$) |
| Middle Mississippi River National Wildlife Refuge | Illinois Missouri | IL | 2000 | 7,000 acres (28 km$^2$) |
| Two Rivers National Wildlife Refuge | Illinois Missouri | IL | 1958 | 8,501 acres (34.40 km$^2$) |
| Big Oaks National Wildlife Refuge | Jefferson County Jennings County Ripley County | IN | 2000 | 50,000 acres (200 km$^2$) |
| Muscatatuck National Wildlife Refuge | Jackson County Jennings County Monroe County | IN | 1966 | 7,880 acres (31.9 km$^2$) |
| Patoka River National Wildlife Refuge and Management Area | Gibson County Pike County | IN | 1994 | 23,962 acres (96.97 km$^2$) |
| DeSoto National Wildlife Refuge | Harrison County Pottawattamie County Washington County, Nebraska | IA | 1958 | 8,362 acres (33.84 km$^2$) |
| Driftless Area National Wildlife Refuge | Iowa Illinois Wisconsin | IA | 1989 | 911 acres (3.69 km$^2$) |
| Iowa Wetland Management District | Covers 15 counties | IA | | 25,000 acres (100 km$^2$) 75 waterfowl production areas |
| Neal Smith National Wildlife Refuge | Jasper County | IA | 1990 | 8,654 acres (35.02 km$^2$) |
| Port Louisa National Wildlife Refuge | Louisa County Mercer County, Illinois | IA | 1958 | 1,078 acres (4.36 km$^2$) |
| Union Slough National Wildlife Refuge | Kossuth County | IA | 1938 | 3,334 acres (13.49 km$^2$) |
| Flint Hills National Wildlife Refuge | Coffey County Lyon County | 1966 | 18,463 acres (74.72 km$^2$) |
| Kirwin National Wildlife Refuge | Phillips County | KS | 1954 | 10,778 acres (43.62 km$^2$) |
| Marais des Cygnes National Wildlife Refuge | Linn County | KS | 1992 | 7,500 acres (30 km$^2$) |
| Quivira National Wildlife Refuge | Reno County Rice County Stafford County | KS | 1955 | 22,135 acres (89.58 km$^2$) |
| Clarks River National Wildlife Refuge | Graves County Marshall County McCracken County | KY | 1997 | 8,040 acres (32.5 km$^2$) |
| Green River National Wildlife Refuge | Henderson County | KY | 2019 | 10 acres (0.040 km$^2$) |
| Atchafalaya National Wildlife Refuge | Iberville Parish St. Martin Parish | LA | 1986 | 15,220 acres (61.6 km$^2$) |
| Bayou Cocodrie National Wildlife Refuge | Concordia Parish | LA | 1992 | 13,200 acres (53 km$^2$) |
| Bayou Sauvage National Wildlife Refuge | New Orleans | LA | 1990 | 22,770 acres (92.1 km$^2$) |
| Bayou Teche National Wildlife Refuge | St. Mary Parish | LA | 2001 | 9,028 acres (36.54 km$^2$) |
| Big Branch Marsh National Wildlife Refuge | St. Tammany Parish | LA | 2001 | 24,000 acres (97 km$^2$) |
| Black Bayou Lake National Wildlife Refuge | Ouachita Parish | LA | 1997 | 4,200 acres (17 km$^2$) |
| Bogue Chitto National Wildlife Refuge | St. Tammany and Washington Parishes, Louisiana and Pearl River County, Mississippi | LA | 1980 | 40,000 acres (160 km$^2$) |
| Breton National Wildlife Refuge | St. Bernard Parish Plaquemines Parish | LA | 1904 | 13,000 acres (53 km$^2$) |
| Cameron Prairie National Wildlife Refuge | Cameron Parish | LA | 1986 | 9,621 acres (38.93 km$^2$) |
| Cat Island National Wildlife Refuge | West Feliciana Parish | LA | 2000 | 9,623 acres (38.94 km$^2$) |
| Catahoula National Wildlife Refuge | Catahoula Parish LaSalle Parish | LA | 1958 | 25,162 acres (101.83 km$^2$) |
| D'Arbonne National Wildlife Refuge | Ouachita Parish Union Parish | LA | 1986 | - |
| Delta National Wildlife Refuge | Plaquemines Parish | LA | 1935 | 49,000 acres (200 km$^2$) |
| Grand Cote National Wildlife Refuge | Avoyelles Parish | LA | 1989 | 6,077 acres (24.59 km$^2$) |
| East Cove National Wildlife Refuge | Cameron Parish | LA | 1937 | 14,927 acres (60.41 km$^2$) |
| Handy Brake National Wildlife Refuge | Morehouse Parish | LA | 1988 | 501 acres (2.03 km$^2$) |
| Lacassine National Wildlife Refuge | Cameron Parish Evangeline Parish | LA | 1937 | 35,000 acres (140 km$^2$) |
| Lake Ophelia National Wildlife Refuge | Avoyelles Parish | LA | 1988 | 17,500 acres (71 km$^2$) |
| Louisiana Wetland Management District | 20 parishes in northern Louisiana | LA | 1990 | 25,710 acres (104.0 km$^2$) |
| Mandalay National Wildlife Refuge | Terrebonne Parish | LA | 1996 | 4,416 acres (17.87 km$^2$) |
| Red River National Wildlife Refuge | Bossier Parish Caddo Parish DeSoto Parish Natchitoches Parish Red River Parish | LA | 2001 | 50,000 acres (200 km$^2$) |
| Sabine National Wildlife Refuge | Cameron Parish | LA | 1937 | 125,790 acres (509.1 km$^2$) |
| Shell Keys National Wildlife Refuge | Iberia Parish | LA | 1907 | - |
| Tensas River National Wildlife Refuge | Franklin Parish Madison Parish Tensas Parish | LA | 1998 | 64,012 acres (259.05 km$^2$) |
| Upper Ouachita National Wildlife Refuge | Morehouse Parish Union Parish | LA | 1978 | 41,430 acres (167.7 km$^2$) |
| Aroostook National Wildlife Refuge | Aroostook County | ME | 1998 | 5,252 acres (21.25 km$^2$) |
| Carlton Pond Waterfowl Production Area | Waldo County | ME | 1966 | 1,055 acres (4.27 km$^2$) |
| Cross Island National Wildlife Refuge (Part of Maine Coastal Islands National Wildlife Refuge) | Washington County | ME | 1980 | 1,700 acres (6.9 km$^2$) |
| Franklin Island National Wildlife Refuge (Part of Maine Coastal Islands National Wildlife Refuge) | Knox County | ME | 1973 | 12 acres (0.049 km$^2$) |
| Moosehorn National Wildlife Refuge | Washington County | ME | 1937 | 28,751 acres (116.35 km$^2$) |
| Petit Manan National Wildlife Refuge (Part of Maine Coastal Islands National Wildlife Refuge) | Hancock County Washington County | ME | 1974 | 6,367 acres (25.77 km$^2$) |
| Pond Island National Wildlife Refuge (Part of Maine Coastal Islands National Wildlife Refuge) | Sagadahoc County | ME | 1973 | 10 acres (0.040 km$^2$) |
| Rachel Carson National Wildlife Refuge | Cumberland County York County | ME | 1966 | 9,125 acres (36.93 km$^2$) |
| Seal Island National Wildlife Refuge (Part of Maine Coastal Islands National Wildlife Refuge) | Knox County | ME | 1972 | 65 acres (0.26 km$^2$) |
| Sunkhaze Meadows National Wildlife Refuge | Penobscot County | ME | 1988 | 11,435 acres (46.28 km$^2$) |
| Blackwater National Wildlife Refuge (Part of Chesapeake Marshlands National Wildlife Refuge Complex) | Dorchester County | MD | 1933 | 28,894.35 acres (116.9313 km$^2$) |
| Eastern Neck National Wildlife Refuge (Part of Chesapeake Marshlands National Wildlife Refuge Complex) | Kent County | MD | 1962 | 2,285 acres (9.25 km$^2$) |
| Glenn Martin National Wildlife Refuge (Part of Chesapeake Marshlands National Wildlife Refuge Complex) | Somerset County Accomack County, Virginia | MD | 1954 | 4,548 acres (18.41 km$^2$) |
| Patuxent Research Refuge | Anne Arundel County Prince George's County | MD | 1936 | 12,800 acres (52 km$^2$) |
| Susquehanna River National Wildlife Refuge (Part of Chesapeake Marshlands National Wildlife Refuge Complex) | Harford County | MD | 1944 | 3.79 acres (0.0153 km$^2$) |
| Assabet River National Wildlife Refuge | Sudbury | MA | 2005 | 2,230 acres (9.0 km$^2$) |
| Great Meadows National Wildlife Refuge | Sudbury | MA | 1944 | 250 acres (1.0 km$^2$) |
| Mashpee National Wildlife Refuge | Mashpee | MA | 1995 | 341.65 acres (1.3826 km$^2$) |
| Massasoit National Wildlife Refuge | Plymouth | MA | 1983 | 195 acres (0.79 km$^2$) |
| Monomoy National Wildlife Refuge | Barnstable County | MA | 1944 | 7,604 acres (30.77 km$^2$) |
| Nantucket National Wildlife Refuge | Nantucket | MA | 1973 | 24 acres (0.097 km$^2$) |
| Nomans Land Island National Wildlife Refuge | Nomans Land | MA | 1998 | 640 acres (2.6 km$^2$) |
| Oxbow National Wildlife Refuge | Middlesex County Worcester County | MA | 1974 | 1,667 acres (6.75 km$^2$) |
| Parker River National Wildlife Refuge | Plum Island | MA | 1942 | 4,662 acres (18.87 km$^2$) |
| Silvio O. Conte National Fish and Wildlife Refuge | Massachusetts Connecticut New Hampshire Vermont | MA | 1997 | 31,216 acres (126.33 km$^2$) |
| Thacher Island National Wildlife Refuge | Thacher Island | MA | 1972 | 22 acres (0.089 km$^2$) |
| Detroit River International Wildlife Refuge | Detroit River Lake Erie | MI | 2001 | 5,868 acres (23.75 km$^2$) |
| Harbor Island National Wildlife Refuge | Lake Huron | MI | 1983 | 695 acres (2.81 km$^2$) |
| Huron National Wildlife Refuge | Lake Superior | MI | 1905 | 147 acres (0.59 km$^2$) |
| Kirtlands Warbler Wildlife Management Area | Lower Peninsula of Michigan | MI | 1980 | 6,684 acres (27.05 km$^2$) |
| Michigan Islands National Wildlife Refuge | Lake Michigan Lake Huron | MI | 1943 | 744 acres (3.01 km$^2$) |
| Michigan Wetland Management District | Lower Peninsula of Michigan | MI | 1980 | 664 acres (2.69 km$^2$) |
| Seney National Wildlife Refuge | Schoolcraft County | MI | 1935 | 95,265 acres (385.52 km$^2$) |
| Shiawassee National Wildlife Refuge | Saginaw County | MI | 1953 | 9,870 acres (39.9 km$^2$) |
| Agassiz National Wildlife Refuge | Marshall County | MN | 1937 | 61,500 acres (249 km$^2$) |
| Big Stone National Wildlife Refuge | Big Stone County Lac qui Parle County | MN | 1975 | 11,586 acres (46.89 km$^2$) |
| Big Stone Wetland Management District | Lincoln County Lyon County | MN | 1975 | 3,000 acres (12 km$^2$) |
| Crane Meadows National Wildlife Refuge | Morrison County | MN | 1992 | 13,540 acres (54.8 km$^2$) |
| Detroit Lakes Wetland Management District | Northwestern Minnesota | MN | 1953 | 53,000 acres (210 km$^2$) |
| Fergus Falls Wetland Management District | West Central Minnesota | MN | 1962 | 72,699 acres (294.20 km$^2$) |
| Glacial Ridge National Wildlife Refuge | Polk County | MN | 2004 | 37,756 acres (152.79 km$^2$) |
| Hamden Slough National Wildlife Refuge | Becker County | MN | 1989 | 5,944 acres (24.05 km$^2$) |
| Litchfield Wetland Management District | Central Minnesota | MN | 1978 | 36,000 acres (150 km$^2$) |
| Mille Lacs National Wildlife Refuge | Mille Lacs County | MN | 1915 | 0.57 acres (0.0023 km$^2$) |
| Minnesota Valley National Wildlife Refuge | Carver County Dakota County Hennepin County Le Sueur County Scott County Sibley County | MN | 1976 | 14,000 acres (57 km$^2$) |
| Minnesota Valley Wetland Management District | Southeastern Minnesota | MN | 1988 | 8,000 acres (32 km$^2$) |
| Morris Wetland Management District | West Central Minnesota | MN | | 50,000 acres (200 km$^2$) |
| Northern Tallgrass Prairie National Wildlife Refuge | Minnesota Iowa | MN | 2000 | 5,000 acres (20 km$^2$) |
| Rice Lake National Wildlife Refuge | Aitkin County | MN | 1935 | 18,208 acres (73.69 km$^2$) |
| Rydell National Wildlife Refuge | Polk County | MN | 1992 | 2,120 acres (8.6 km$^2$) |
| Sherburne National Wildlife Refuge | Sherburne County | MN | 1965 | 30,700 acres (124 km$^2$) |
| Tamarac National Wildlife Refuge | Becker County | MN | 1938 | 42,724 acres (172.90 km$^2$) |
| Upper Mississippi River National Wildlife and Fish Refuge | Upper Mississippi River in: Illinois Iowa Minnesota Wisconsin | MN | 1924 | 240,000 acres (970 km$^2$) |
| Windom Wetland Management District | South Central Minnesota | MN | - | 14,000 acres (57 km$^2$) |
| Coldwater River National Wildlife Refuge | Quitman County Tallahatchie County | MS | 2000 | 2,069 acres (8.37 km$^2$) |
| Dahomey National Wildlife Refuge | Bolivar County | MS | 1990 | 9,691 acres (39.22 km$^2$) |
| Grand Bay National Wildlife Refuge | Jackson County Mobile County, Alabama | MS | 1992 | 32,000 acres (130 km$^2$) |
| Hillside National Wildlife Refuge | Holmes County | MS | 1975 | 15,572 acres (63.02 km$^2$) |
| Holt Collier National Wildlife Refuge | Washington County | MS | 2004 | 1,400 acres (5.7 km$^2$) |
| Mathews Brake National Wildlife Refuge | Leflore County | MS | 1980 | 2,418 acres (9.79 km$^2$) |
| Mississippi Sandhill Crane National Wildlife Refuge | Jackson County | MS | 1975 | 19,000 acres (77 km$^2$) |
| Morgan Brake National Wildlife Refuge | Holmes County | MS | 1977 | 7,383 acres (29.88 km$^2$) |
| Panther Swamp National Wildlife Refuge | Yazoo County | MS | 1978 | 38,697 acres (156.60 km$^2$) |
| Sam D. Hamilton Noxubee National Wildlife Refuge | Noxubee County Oktibbeha County Winston County | MS | 1940 | 48,000 acres (190 km$^2$) |
| St. Catherine Creek National Wildlife Refuge | Adams County | MS | 1990 | 34,256 acres (138.63 km$^2$) |
| Tallahatchie National Wildlife Refuge | Grenada County Tallahatchie County | MS | 1990 | 4,199 acres (16.99 km$^2$) |
| Theodore Roosevelt National Wildlife Refuge | Holmes County Humphreys County Leflore County Sharkey County Warren County Washington County | MS | 2004 | - |
| Yazoo National Wildlife Refuge | Washington County | MS | 1936 | 12,941 acres (52.37 km$^2$) |
| Big Muddy National Fish and Wildlife Refuge | Missouri River | MO | 1994 | 16,700 acres (68 km$^2$) |
| Clarence Cannon National Wildlife Refuge | Pike County | MO | 1964 | 3,750 acres (15.2 km$^2$) |
| Great River National Wildlife Refuge | Clark County Lewis County Adams County, Illinois Pike County, Illinois | MO | 1958 | 11,600 acres (47 km$^2$) |
| Loess Bluffs National Wildlife Refuge | Holt County | MO | 1935 | 7,415 acres (30.01 km$^2$) |
| Mingo National Wildlife Refuge | Stoddard County Wayne County | MO | 1944 | 21,676 acres (87.72 km$^2$) |
| Ozark Cavefish National Wildlife Refuge | Lawrence County | MO | 1991 | 40 acres (0.16 km$^2$) |
| Pilot Knob National Wildlife Refuge | Iron County | MO | 1987 | 90 acres (0.36 km$^2$) |
| Swan Lake National Wildlife Refuge | Chariton County | MO | 1937 | 10,795 acres (43.69 km$^2$) |
| Benton Lake National Wildlife Refuge | Cascade County | MT | 1929 | 12,459 acres (50.42 km$^2$) |
| Benton Lake Wetland Management District | Montana | MT | 1975 | 16,000 acres (65 km$^2$) |
| Black Coulee National Wildlife Refuge | Blaine County | MT | 1938 | 1,308 acres (5.29 km$^2$) |
| Bowdoin National Wildlife Refuge | Phillips County | MT | 1936 | 15,551 acres (62.93 km$^2$) |
| Charles M. Russell National Wildlife Refuge | Fergus County Garfield County McCone County Petroleum County Phillips County Valley County | MT | 1936 | 915,814 acres (3,706.17 km$^2$) |
| Creedman Coulee National Wildlife Refuge | Hill County | MT | 1941 | 2,728 acres (11.04 km$^2$) |
| Grass Lake National Wildlife Refuge (formerly Halfbreed Lake NWR) | Stillwater County | MT | 1987 | 4,318 acres (17.47 km$^2$) |
| Hailstone National Wildlife Refuge | Stillwater County | MT | 1942 | 2,700 acres (11 km$^2$) |
| Hewitt Lake National Wildlife Refuge | Phillips County | MT | 1938 | 1,360 acres (5.5 km$^2$) |
| Lake Mason National Wildlife Refuge | Musselshell County | MT | 1941 | 16,814 acres (68.04 km$^2$) |
| Lake Thibadeau National Wildlife Refuge | Hill County | MT | 1937 | 3,868 acres (15.65 km$^2$) |
| Lamesteer National Wildlife Refuge | Wibaux County | MT | 1942 | 800 acres (3.2 km$^2$) |
| Lee Metcalf National Wildlife Refuge | Ravalli County | MT | 1964 | 2,800 acres (11 km$^2$) |
| Lost Trail National Wildlife Refuge | Flathead County | MT | 1999 | 8,834 acres (35.75 km$^2$) |
| Medicine Lake National Wildlife Refuge | Sheridan County Roosevelt County | MT | 1935 | 31,533 acres (127.61 km$^2$) |
| Ninepipe National Wildlife Refuge | Lake County | MT | 1921 | 4,027 acres (16.30 km$^2$) |
| Northwest Montana Wetland Management District | Montana | MT | 1970 | 14,752 acres (59.70 km$^2$) |
| Pablo National Wildlife Refuge | Lake County | MT | 1921 | 2,473 acres (10.01 km$^2$) |
| Red Rock Lakes National Wildlife Refuge | Beaverhead County | MT | 1932 | 65,810 acres (266.3 km$^2$) |
| Swan River National Wildlife Refuge | Lake County | MT | 1973 | 1,568 acres (6.35 km$^2$) |
| UL Bend National Wildlife Refuge | Phillips County | MT | 1969 | 56,048 acres (226.82 km$^2$) |
| War Horse National Wildlife Refuge | Petroleum County | MT | 1958 | 3,392 acres (13.73 km$^2$) |
| Boyer Chute National Wildlife Refuge | Washington County | NE | 1992 | 4,040 acres (16.3 km$^2$) |
| Crescent Lake National Wildlife Refuge | Garden County | NE | 1931 | 45,818 acres (185.42 km$^2$) |
| Fort Niobrara National Wildlife Refuge | Cherry County | NE | 1912 | 19,131 acres (77.42 km$^2$) |
| John and Louise Seier National Wildlife Refuge | Rock County | NE | 1999 | 2,400 acres (9.7 km$^2$) |
| North Platte National Wildlife Refuge | Scotts Bluff County | NE | 1916 | 5,047 acres (20.42 km$^2$) |
| Rainwater Basin Wetland Management District | South Central Nebraska | NE | 1963 | 22,864 acres (92.53 km$^2$) |
| Valentine National Wildlife Refuge | Cherry County | NE | 1935 | 71,516 acres (289.41 km$^2$) |
| Anaho Island National Wildlife Refuge | Washoe County | NV | 1913 | 247 acres (1.00 km$^2$) |
| Ash Meadows National Wildlife Refuge (Managed as part of the Desert National Wildlife Refuge Complex, headquartered in Las Vegas) | Nye County | NV | 1984 | 23,000 acres (93 km$^2$) |
| Desert National Wildlife Refuge (Managed as part of the Desert National Wildlife Refuge Complex, headquartered in Las Vegas) | Clark County Lincoln County | NV | 1936 | 1,615,000 acres (6,540 km$^2$) |
| Fallon National Wildlife Refuge | Churchill County | NV | 1931 | 15,000 acres (61 km$^2$) |
| Moapa Valley National Wildlife Refuge (Managed as part of the Desert National Wildlife Refuge Complex, headquartered in Las Vegas) | Clark County | NV | 1979 | 106 acres (0.43 km$^2$) |
| Pahranagat National Wildlife Refuge (Managed as part of the Desert National Wildlife Refuge Complex, headquartered in Las Vegas) | Lincoln County | NV | 1963 | 5,380 acres (21.8 km$^2$) |
| Ruby Lake National Wildlife Refuge | Elko County White Pine County | NV | 1938 | 37,632 acres (152.29 km$^2$) |
| Sheldon National Wildlife Refuge (Managed as part of the Sheldon-Hart Mountain National Wildlife Refuge Complex, headquartered in Lakeview, Oregon) | Humboldt County Washoe County Lake County, Oregon | NV | 1931 | 573,504 acres (2,320.89 km$^2$) |
| Stillwater National Wildlife Refuge | Churchill County | NV | 1949 | 79,570 acres (322.0 km$^2$) |
| Great Bay National Wildlife Refuge | Rockingham County | NH | 1992 | 1,116 acres (4.52 km$^2$) |
| John Hay National Wildlife Refuge | Merrimack County | NH | 1987 | 164 acres (0.66 km$^2$) |
| Umbagog National Wildlife Refuge | Coös County Oxford County, Maine | NH | 1992 | 25,650 acres (103.8 km$^2$) |
| Wapack National Wildlife Refuge | Hillsborough County | NH | 1972 | 1,672 acres (6.77 km$^2$) |
| Cape May National Wildlife Refuge | Cape May County | NJ | 1989 | 21,200 acres (86 km$^2$) |
| Edwin B. Forsythe National Wildlife Refuge | Atlantic County Ocean County | NJ | 1984 | 47,437 acres (191.97 km$^2$) |
| Great Swamp National Wildlife Refuge | Morris County | NJ | 1960 | 7,800 acres (32 km$^2$) |
| Supawna Meadows National Wildlife Refuge | Salem County | NJ | 1974 | 3,230 acres (13.1 km$^2$) |
| Wallkill River National Wildlife Refuge | Sussex County Orange County, New York | NJ | 1990 | 5,100 acres (21 km$^2$) |
| Bitter Lake National Wildlife Refuge | Chaves County | NM | 1937 | 24,563 acres (99.40 km$^2$) |
| Bosque del Apache National Wildlife Refuge | Socorro County | NM | 1939 | 57,331 acres (232.01 km$^2$) |
| Grulla National Wildlife Refuge | Roosevelt County Bailey County, Texas | NM | 1969 | 3,264 acres (13.21 km$^2$) |
| Las Vegas National Wildlife Refuge | San Miguel County | NM | 1965 | 8,672 acres (35.09 km$^2$) |
| Rio Mora National Wildlife Refuge | Mora County | NM | 2012 | 4,224 acres (17.09 km$^2$) |
| Maxwell National Wildlife Refuge | Colfax County | NM | 1965 | 3,699 acres (14.97 km$^2$) |
| San Andres National Wildlife Refuge | Doña Ana County | NM | 1941 | 57,215 acres (231.54 km$^2$) |
| Sevilleta National Wildlife Refuge | Socorro County | NM | 1973 | 230,000 acres (930 km$^2$) |
| Valle de Oro National Wildlife Refuge | Bernalillo County | NM | 2012 | 570 acres (2.3 km$^2$) |
| Amagansett National Wildlife Refuge (Part of the Long Island National Wildlife Refuge Complex) | Suffolk County | NY | 1968 | 36 acres (0.15 km$^2$) |
| Conscience Point National Wildlife Refuge (Part of the Long Island National Wildlife Refuge Complex) | Suffolk County | NY | 1971 | 60 acres (0.24 km$^2$) |
| Elizabeth A. Morton National Wildlife Refuge (Part of the Long Island National Wildlife Refuge Complex) | Suffolk County | NY | 1954 | 187 acres (0.76 km$^2$) |
| Iroquois National Wildlife Refuge | Genesee County Orleans County | NY | 1958 | 10,828 acres (43.82 km$^2$) |
| Montezuma National Wildlife Refuge | Seneca County | NY | 1937 | 10,004 acres (40.48 km$^2$) |
| Oyster Bay National Wildlife Refuge (Part of the Long Island National Wildlife Refuge Complex) | Nassau County | NY | 1968 | 3,209 acres (12.99 km$^2$) |
| Sayville National Wildlife Refuge (Part of the Long Island National Wildlife Refuge Complex) | Suffolk County | NY | 1992 | 127 acres (0.51 km$^2$) |
| Seatuck National Wildlife Refuge (Part of the Long Island National Wildlife Refuge Complex) | Suffolk County | NY | 1968 | 196 acres (0.79 km$^2$) |
| Shawangunk Grasslands National Wildlife Refuge | Ulster County | NY | 1999 | 597 acres (2.42 km$^2$) |
| Target Rock National Wildlife Refuge (Part of the Long Island National Wildlife Refuge Complex) | Suffolk County | NY | 1967 | 80 acres (0.32 km$^2$) |
| Wertheim National Wildlife Refuge (Part of the Long Island National Wildlife Refuge Complex) | Suffolk County | NY | 1947 | 2,550 acres (10.3 km$^2$) |
| Alligator River National Wildlife Refuge (Part of the North Carolina Coastal Plain National Wildlife Refuge Complex) | Dare County Hyde County | NC | 1984 | 152,000 acres (620 km$^2$) |
| Cedar Island National Wildlife Refuge (Part of the Mattamuskeet, Swanquarter and Cedar Island National Wildlife Refuge Complex) | Carteret County | NC | 1964 | 14,480 acres (58.6 km$^2$) |
| Currituck National Wildlife Refuge (Part of the North Carolina Coastal Plain National Wildlife Refuge Complex) | Currituck County | NC | 1984 | 8,316 acres (33.65 km$^2$) |
| Mackay Island National Wildlife Refuge (Part of the North Carolina Coastal Plain National Wildlife Refuge Complex) | Currituck County Virginia Beach, Virginia | NC | 1960 | 8,231 acres (33.31 km$^2$) |
| Mattamuskeet National Wildlife Refuge (Part of the Mattamuskeet, Swanquarter and Cedar Island National Wildlife Refuge Complex) | Hyde County | NC | 1934 | 50,173 acres (203.04 km$^2$) |
| Mountain Bogs National Wildlife Refuge | Ashe County | NC | 2015 | 39 acres (0.16 km$^2$) |
| Pea Island National Wildlife Refuge (Part of the North Carolina Coastal Plain National Wildlife Refuge Complex) | Dare County | NC | 1938 | 31,534 acres (127.61 km$^2$) |
| Pee Dee National Wildlife Refuge | Anson County Richmond County | NC | 1963 | 8,443 acres (34.17 km$^2$) |
| Pocosin Lakes National Wildlife Refuge (Part of the North Carolina Coastal Plain National Wildlife Refuge Complex) | Hyde County Tyrrell County Washington County | NC | 1963 | 110,106 acres (445.58 km$^2$) |
| Roanoke River National Wildlife Refuge (Part of the North Carolina Coastal Plain National Wildlife Refuge Complex) | Bertie County | NC | 1989 | 20,978 acres (84.89 km$^2$) |
| Swanquarter National Wildlife Refuge (Part of the Mattamuskeet, Swanquarter and Cedar Island National Wildlife Refuge Complex) | Hyde County | NC | 1932 | 16,411 acres (66.41 km$^2$) |
| Appert Lake National Wildlife Refuge | Emmons County | ND | 1930s | 1,978 acres (8.00 km$^2$) Easement on private land |
| Ardoch National Wildlife Refuge (Managed by the Devils Lake Wetland Management District) | Walsh County | ND | 1939 | 3,084 acres (12.48 km$^2$) Easement on private land |
| Arrowwood National Wildlife Refuge (Managed by the Arrowwood National Wildlife Refuge Complex) | Stutsman County | ND | 1935 | 15,934 acres (64.48 km$^2$) |
| Arrowwood Wetland Management District (Managed by the Arrowwood National Wildlife Refuge Complex) | Eddy County Foster County | ND | 1961 | 6,162 acres (24.94 km$^2$) |
| Audubon National Wildlife Refuge (Managed by the Audubon National Wildlife Refuge Complex) | McLean County | ND | 1956 (renamed 1967) | 14,735 acres (59.63 km$^2$) |
| Audubon Wetland Management District (Managed by the Audubon National Wildlife Refuge Complex) | McLean County Sheridan County | ND | 1950s | 155,000 acres (630 km$^2$) 84 waterfowl production areas |
| Bone Hill National Wildlife Refuge | LaMoure County | ND | 1939 | 1,978 acres (8.00 km$^2$) Easement on private land |
| Brumba National Wildlife Refuge | Towner County | ND | 1939 | Easement on private land |
| Buffalo Lake National Wildlife Refuge (North Dakota) | Pierce County | ND | | Easement on private land |
| Camp Lake National Wildlife Refuge | McLean County | ND | | 585 acres (2.37 km$^2$) Easement on private land |
| Canfield Lake National Wildlife Refuge | Burleigh County | ND | | 1,978 acres (8.00 km$^2$) Easement on private land |
| Chase Lake National Wildlife Refuge (Managed by the Arrowwood National Wildlife Refuge Complex) | Stutsman County | ND | 1908 | 4,385 acres (17.75 km$^2$) |
| Chase Lake Wetland Management District (Managed by the Arrowwood National Wildlife Refuge Complex) | Stutsman County Wells County | ND | 1993 | 162,925 acres (659.33 km$^2$) 135 waterfowl production areas |
| Cottonwood Lake National Wildlife Refuge | McHenry County | ND | 1939 | Easement on private land |
| Crosby Wetland Management District (Managed by the Lostwood Wetland Management District Complex) | Burke County Divide County Williams County | ND | 1962 | 21,219 acres (85.87 km$^2$) 90 waterfowl production areas |
| Dakota Lake National Wildlife Refuge | Dickey County | ND | 1939 | 2,784 acres (11.27 km$^2$) Easement on private land |
| Des Lacs National Wildlife Refuge (Managed by the Lostwood Wetland Management District Complex) | Burke County Ward County | ND | 1935 | 19,500 acres (79 km$^2$) |
| Devils Lake Wetland Management District (Managed by the Devils Lake Wetland Management District) | Benson County Cavalier County Grand Forks County Pembina County Ramsey County Towner County Walsh County | ND | | 258,000 acres (1,040 km$^2$) 257 waterfowl production areas |
| Florence Lake National Wildlife Refuge (Managed by the Long Lake National Wildlife Refuge Complex) | Burleigh County | ND | 1939 | 1,888.2 acres (7.641 km$^2$) |
| Half-Way Lake National Wildlife Refuge | Stutsman County | ND | 1939 | 160 acres (0.65 km$^2$) Easement on private land |
| Hiddenwood National Wildlife Refuge | McLean County | ND | 1939 | 675 acres (2.73 km$^2$) Easement on private land |
| Hobart Lake National Wildlife Refuge | Barnes County | ND | 1939 | 2,077 acres (8.41 km$^2$) Easement on private land |
| Hutchinson Lake National Wildlife Refuge | Kidder County | ND | | Easement on private land |
| J. Clark Salyer National Wildlife Refuge (Managed by the J. Clark Salyer National Wildlife Refuge Complex) | Bottineau County McHenry County | ND | 1935 | 58,700 acres (238 km$^2$) |
| J. Clark Salyer Wetland Management District (Managed by the J. Clark Salyer National Wildlife Refuge Complex) | Bottineau County McHenry County Pierce County Renville County Rolette County | ND | 1966 | 185,090 acres (749.0 km$^2$) |
| Johnson Lake National Wildlife Refuge | Eddy County Nelson County | ND | 1939 | 2,002 acres (8.10 km$^2$) Easement on private land |
| Kellys Slough National Wildlife Refuge (Managed by the Devils Lake Wetland Management District) | Grand Forks County | ND | 1936 | 2,146 acres (8.68 km$^2$) |
| Kulm Wetland Management District | Dickey County LaMoure County Logan County McIntosh County | ND | 1971 | 279,184 acres (1,129.82 km$^2$) 200 waterfowl production areas |
| Lake Alice National Wildlife Refuge (Managed by the Devils Lake Wetland Management District) | Ramsey County | ND | 1935 | 8,512 acres (34.45 km$^2$) |
| Lake George National Wildlife Refuge | Stutsman County | ND | 1939 | Easement on private land |
| Lake Ilo National Wildlife Refuge (Managed by the Audubon National Wildlife Refuge Complex) | Dunn County | ND | 1939 | 4,000 acres (16 km$^2$) |
| Lake Nettie National Wildlife Refuge (Managed by the Audubon National Wildlife Refuge Complex) | McLean County | ND | 1939 | 1,285 acres (5.20 km$^2$) |
| Lake Otis National Wildlife Refuge | McLean County | ND | | 320 acres (1.3 km$^2$) Easement on private land |
| Lake Patricia National Wildlife Refuge | Morton County | ND | 1939 | 800 acres (3.2 km$^2$) Easement on private land |
| Lake Zahl National Wildlife Refuge (Managed by the Lostwood Wetland Management District Complex) | Williams County | ND | 1939 | 3,823 acres (15.47 km$^2$) |
| Lambs Lake National Wildlife Refuge | Nelson County | ND | 1939 | Easement on private land |
| Little Goose National Wildlife Refuge | Grand Forks County | ND | | Easement on private land |
| Long Lake National Wildlife Refuge (Managed by the Long Lake National Wildlife Refuge Complex) | Burleigh County | ND | 1932 | 22,300 acres (90 km$^2$) |
| Long Lake Wetland Management District (Managed by the Long Lake National Wildlife Refuge Complex) | Kidder County Burleigh County Emmons County | ND | 1950s | 100,000 acres (400 km$^2$) |
| Lords Lake National Wildlife Refuge | Bottineau County | ND | | Easement on private land |
| Lost Lake National Wildlife Refuge | McLean County | ND | | 960 acres (3.9 km$^2$) Easement on private land |
| Lostwood National Wildlife Refuge (Managed by the Lostwood Wetland Management District Complex) | Burke County Mountrail County | ND | 1940 | 27,589 acres (111.65 km$^2$) |
| Lostwood Wetland Management District (Managed by the Lostwood Wetland Management District Complex) | Mountrail County Ward County | ND | 1961 | Scattered small tracts |
| Maple River National Wildlife Refuge | Dickey County | ND | 1939 | 414 acres (1.68 km$^2$) Federal land 1,120 acres (4.5 km$^2$) Easement on private land |
| McLean National Wildlife Refuge | McLean County | ND | 1939 (as Lake Susie NWR) | 760 acres (3.1 km$^2$) |
| Pleasant Lake National Wildlife Refuge | Benson County | ND | 1939 | 1,000 acres (4.0 km$^2$) Easement on private land |
| Pretty Rock National Wildlife Refuge | Grant County | ND | 760 | 800 acres (3.2 km$^2$) Easement on private land |
| Rabb Lake National Wildlife Refuge | Rolette County | ND | | Easement on private land |
| Rock Lake National Wildlife Refuge | Towner County | ND | 1939 | Easement on private land |
| Rose Lake National Wildlife Refuge | Nelson County | ND | 1948 | 872.1 acres (3.529 km$^2$) Easement on private land |
| School Section Lake National Wildlife Refuge | Rolette County | ND | 1935 | Easement on private land |
| Shell Lake National Wildlife Refuge (Managed by the Lostwood Wetland Management District Complex) | Mountrail County | ND | 1939 | 1,835 acres (7.43 km$^2$) |
| Sibley Lake National Wildlife Refuge | Griggs County | ND | | 1,077 acres (4.36 km$^2$) Easement on private land |
| Silver Lake National Wildlife Refuge | Benson County | ND | 1938 | 398.76 acres (1.6137 km$^2$) Easement on private land |
| Slade National Wildlife Refuge (Managed by the Long Lake National Wildlife Refuge Complex) | Kidder County | ND | 1940 | 3,000 acres (12 km$^2$) |
| Snyder Lake National Wildlife Refuge | Towner County | ND | 1941 | Easement on private land |
| Springwater National Wildlife Refuge | Emmons County | ND | | Easement on private land |
| Stewart Lake National Wildlife Refuge (Managed by the Audubon National Wildlife Refuge Complex) | Slope County | ND | 1941 | 730 acres (3.0 km$^2$) federal land 1,600 acres (6.5 km$^2$) easement on private land |
| Stoney Slough National Wildlife Refuge | Barnes County | ND | 1941 | 1,120 acres (4.5 km$^2$) Federal land 880 acres (3.6 km$^2$) Easement on private land |
| Storm Lake National Wildlife Refuge | Sargent County | ND | | Easement on private land |
| Stump Lake National Wildlife Refuge | Nelson County | ND | 1905 | Currently under water |
| Sunburst Lake National Wildlife Refuge | Emmons County | ND | | 328 acres (1.33 km$^2$) Easement on private land |
| Tewaukon National Wildlife Refuge (Managed by the Souris River Basin National Wildlife Refuge Complex) | Sargent County | ND | 1945 | 8,343 acres (33.76 km$^2$) |
| Tewaukon Wetland Management District (Managed by the Tewaukon National Wildlife Refuge Complex) | Ransom County Richland County Sargent County | ND | | 49,000 acres (200 km$^2$) |
| Tomahawk National Wildlife Refuge | Barnes County | ND | | 440 acres (1.8 km$^2$) Easement on private land |
| Upper Souris National Wildlife Refuge (Managed by the Souris River Basin National Wildlife Refuge Complex) | Renville County Ward County | ND | 1932 | 32,092 acres (129.87 km$^2$) |
| Valley City Wetland Management District (Managed by the Arrowwood National Wildlife Refuge Complex) | Barnes County Cass County Griggs County Steele County Traill County | ND | 1971 | 89,000 acres (360 km$^2$) 82 waterfowl production areas |
| White Lake National Wildlife Refuge (Managed by the Audubon National Wildlife Refuge Complex) | Slope County | ND | 1941 | 1,040 acres (4.2 km$^2$) |
| White Horse Hill National Game Preserve (Managed by the Devils Lake Wetland Management District) | Benson County | ND | 1904 | 1,674 acres (6.77 km$^2$) |
| Wild Rice Lake National Wildlife Refuge | Sargent County | ND | | Easement on private land |
| Willow Lake National Wildlife Refuge | Rolette County | ND | | Easement on private land |
| Wintering River National Wildlife Refuge | McHenry County | ND | | Easement on private land |
| Wood Lake National Wildlife Refuge | Benson County | ND | 1935 | Easement on private land |
| Cedar Point National Wildlife Refuge (Managed by the Ottawa National Wildlife Refuge Complex) | Lucas County | OH | 1965 | 2,449.77 acres (9.9139 km$^2$) |
| Ottawa National Wildlife Refuge (Managed by the Ottawa National Wildlife Refuge Complex) | Lucas County Ottawa County | OH | 1961 | 6,857.91 acres (27.7530 km$^2$) |
| West Sister Island National Wildlife Refuge (Managed by the Ottawa National Wildlife Refuge Complex) | Lucas County | OH | 1937 | 77 acres (0.31 km$^2$) |
| Deep Fork National Wildlife Refuge | Okmulgee County | OK | 1993 | 9,748 acres (39.45 km$^2$) |
| Little River National Wildlife Refuge | McCurtain County | OK | 1987 | 13,660 acres (55.3 km$^2$) |
| Optima National Wildlife Refuge | Texas County | OK | 1975 | 4,333 acres (17.54 km$^2$) |
| Ozark Plateau National Wildlife Refuge | Adair County Cherokee County Delaware County Ottawa County | OK | 1986 | 4,200 acres (17 km$^2$) |
| Salt Plains National Wildlife Refuge | Alfalfa County | OK | 1930 | 32,197 acres (130.30 km$^2$) |
| Sequoyah National Wildlife Refuge | Haskell County Muskogee County Sequoyah County | OK | 1970 | 20,800 acres (84 km$^2$) |
| Tishomingo National Wildlife Refuge | Johnston County Marshall County | OK | 1946 | 16,464 acres (66.63 km$^2$) |
| Washita National Wildlife Refuge | Custer County | OK | 1961 | 8,075 acres (32.68 km$^2$) |
| Wichita Mountains Wildlife Refuge | Comanche County | OK | 1901 | 59,020 acres (238.8 km$^2$) |
| Ankeny National Wildlife Refuge | Marion County | OR | 1965 | 2,796 acres (11.32 km$^2$) |
| Bandon Marsh National Wildlife Refuge | Coos County | OR | 1983 | 864 acres (3.50 km$^2$) |
| Baskett Slough National Wildlife Refuge | Polk County | OR | 1965 | 2,492 acres (10.08 km$^2$) |
| Bear Valley National Wildlife Refuge | Klamath County | OR | 1978 | 4,200 acres (17 km$^2$) |
| Cape Meares National Wildlife Refuge | Tillamook County | OR | 1938 | 138.51 acres (0.5605 km$^2$) |
| Cold Springs National Wildlife Refuge | Umatilla County | OR | 1909 | 3,117 acres (12.61 km$^2$) |
| Deer Flat National Wildlife Refuge | Malheur County Baker County Canyon County, Idaho Owyhee County, Idaho Payette County, Idaho Washington County, Idaho | OR | 1909 | 10,548 acres (42.69 km$^2$) |
| Hart Mountain National Antelope Refuge | Lake County | OR | 1936 | 270,608 acres (1,095.11 km$^2$) |
| Julia Butler Hansen Refuge for the Columbian White-Tailed Deer | Clatsop County Columbia County Wahkiakum County, Washington | OR | 1972 | 5,600 acres (23 km$^2$) |
| Klamath Marsh National Wildlife Refuge | Klamath County | OR | 1958 | 40,885 acres (165.46 km$^2$) |
| Lewis and Clark National Wildlife Refuge | Clatsop County | OR | 1972 | 12,167 acres (49.24 km$^2$) |
| Lower Klamath National Wildlife Refuge | Klamath County Siskiyou County, California | OR | 1908 | 187,757 acres (759.83 km$^2$) |
| Malheur National Wildlife Refuge | Harney County | OR | 1908 | 187,757 acres (759.83 km$^2$) |
| McKay Creek National Wildlife Refuge | Umatilla County | OR | 1991 | 1,836.5 acres (7.432 km$^2$) |
| Nestucca Bay National Wildlife Refuge | Tillamook County | OR | 1991 | 1,202 acres (4.86 km$^2$) |
| Oregon Islands National Wildlife Refuge | Oregon Coast | OR | 1935 | 1,083 acres (4.38 km$^2$) |
| Sheldon National Wildlife Refuge | Lake County Humboldt County, Nevada Washoe County, Nevada | OR | 1931 | 573,504 acres (2,320.89 km$^2$) |
| Siletz Bay National Wildlife Refuge | Lincoln County | OR | 1991 | 568 acres (2.30 km$^2$) |
| Three Arch Rocks National Wildlife Refuge | Tillamook County | OR | 1907 | 15 acres (0.061 km$^2$) |
| Tualatin River National Wildlife Refuge | Washington County Yamhill County | OR | 1992 | 1,856 acres (7.51 km$^2$) |
| Umatilla National Wildlife Refuge | Morrow County Benton County, Washington | OR | 1969 | 23,783 acres (96.25 km$^2$) |
| Upper Klamath National Wildlife Refuge | Klamath County | OR | 1928 | 14,400 acres (58 km$^2$) |
| Wapato Lake National Wildlife Refuge | Washington County Yamhill County | OR | 2013 | 4,310 acres (17.4 km$^2$) |
| William L. Finley National Wildlife Refuge | Benton County | OR | 1964 | 5,706 acres (23.09 km$^2$) |
| Cherry Valley National Wildlife Refuge | Monroe County | PA | 2008 | 185 acres (0.75 km$^2$) |
| Erie National Wildlife Refuge | Crawford County | PA | 1959 | 8,800 acres (36 km$^2$) |
| John Heinz National Wildlife Refuge at Tinicum | Delaware County Philadelphia County | PA | 1972 | 1,200 acres (4.9 km$^2$) |
| Cabo Rojo National Wildlife Refuge (Managed by the Caribbean Islands National Wildlife complex) | Cabo Rojo | PR | 1974 | 1,836 acres (7.43 km$^2$) |
| Culebra National Wildlife Refuge (Managed by the Caribbean Islands National Wildlife complex) | Isla Culebra | PR | 1909 | 1,450 acres (5.9 km$^2$) |
| Desecheo National Wildlife Refuge (Managed by the Caribbean Islands National Wildlife complex) | Mayagüez | PR | 1976 | 360 acres (1.5 km$^2$) |
| Laguna Cartagena National Wildlife Refuge (Managed by the Caribbean Islands National Wildlife complex) | Lajas | PR | 1989 | 1,059 acres (4.29 km$^2$) |
| Vieques National Wildlife Refuge (Managed by the Caribbean Islands National Wildlife complex) | Isla de Vieques | PR | 2001 | 17,771 acres (71.92 km$^2$) |
| Block Island National Wildlife Refuge (Managed by the Rhode Island National Wildlife Refuge Complex) | Washington County | RI | 1973 | 127 acres (0.51 km$^2$) |
| John H. Chafee National Wildlife Refuge (Managed by the Rhode Island National Wildlife Refuge Complex) | Washington County | RI | 1988 | 317 acres (1.28 km$^2$) |
| Ninigret National Wildlife Refuge (Managed by the Rhode Island National Wildlife Refuge Complex) | Washington County | RI | 1970 | 900 acres (3.6 km$^2$) |
| Sachuest Point National Wildlife Refuge (Managed by the Rhode Island National Wildlife Refuge Complex) | Newport County | RI | 1970 | 242 acres (0.98 km$^2$) |
| Trustom Pond National Wildlife Refuge (Managed by the Rhode Island National Wildlife Refuge Complex) | Washington County | RI | 1974 | 787 acres (3.18 km$^2$) |
| Cape Romain National Wildlife Refuge (Part of the South Carolina Lowcountry National Wildlife Refuge Complex) | Charleston County | SC | 1932 | 66,287 acres (268.25 km$^2$) |
| Carolina Sandhills National Wildlife Refuge | Chesterfield County | SC | 1939 | 45,348 acres (183.52 km$^2$) |
| Ernest F. Hollings ACE Basin National Wildlife Refuge (Part of the South Carolina Lowcountry National Wildlife Refuge Complex) | Beaufort County Charleston County Colleton County Hampton County | SC | 1990 | 11,815 acres (47.81 km$^2$) |
| Pinckney Island National Wildlife Refuge (Part of the Savannah Coastal Refuges Complex) | Beaufort County | SC | 1975 | 4,053 acres (16.40 km$^2$) |
| Santee National Wildlife Refuge (Part of the Savannah Coastal Refuges Complex) | Clarendon County | SC | 1941 | 15,000 acres (61 km$^2$) |
| Tybee National Wildlife Refuge (Part of the Savannah Coastal Refuges Complex) | Jasper County | SC | 1938 | 100 acres (0.40 km$^2$) |
| Waccamaw National Wildlife Refuge (Part of the South Carolina Lowcountry National Wildlife Refuge Complex) | Georgetown County Horry County Marion County | SC | 1997 | 22,931 acres (92.80 km$^2$) |
| Bear Butte National Wildlife Refuge | Meade County | SD | 1935 | 374 acres (1.51 km$^2$) |
| Huron Wetland Management District | Central South Dakota | SD | 1992 | 17,518 acres (70.89 km$^2$) |
| Karl E. Mundt National Wildlife Refuge | Gregory County Boyd County, Nebraska | SD | 1974 | 1,085 acres (4.39 km$^2$) |
| Lacreek National Wildlife Refuge | Bennett County | SD | 1935 | 16,410 acres (66.4 km$^2$) |
| Lake Andes National Wildlife Refuge | Charles Mix County | SD | 1936 | 5,638 acres (22.82 km$^2$) |
| Lake Andes Wetland Management District | Southeast South Dakota | SD | 1961 | 82,731 acres (334.80 km$^2$) |
| Madison Wetland Management District | East Central South Dakota | SD | 1969 | 129,700 acres (525 km$^2$) |
| Sand Lake National Wildlife Refuge | Brown County | SD | 1935 | 21,498 acres (87.00 km$^2$) |
| Sand Lake Wetland Management District | North Central South Dakota | SD | 1961 | 45,000 acres (180 km$^2$) |
| Waubay National Wildlife Refuge | Day County | SD | 1935 | 4,650 acres (18.8 km$^2$) |
| Waubay Wetland Management District | Northeast South Dakota | SD | 1935 | 40,000 acres (160 km$^2$) |
| Chickasaw National Wildlife Refuge (Part of the West Tennessee National Wildlife Refuge Complex) | Lauderdale County | TN | 1985 | 25,006 acres (101.20 km$^2$) |
| Cross Creeks National Wildlife Refuge | Stewart County | TN | 1962 | 8,862 acres (35.86 km$^2$) |
| Hatchie National Wildlife Refuge | Hardeman County Haywood County | TN | 1964 | 11,556 acres (46.77 km$^2$) |
| Lake Isom National Wildlife Refuge (Part of the West Tennessee National Wildlife Refuge Complex) | Lake County | TN | 1938 | 1,846 acres (7.47 km$^2$) |
| Lower Hatchie National Wildlife Refuge (Part of the West Tennessee National Wildlife Refuge Complex) | Lauderdale County Tipton County | TN | 1980 | 9,451 acres (38.25 km$^2$) |
| Paint Rock River National Wildlife Refuge | Jackson County | TN | 2023 | 87 acres (0.35 km$^2$) |
| Reelfoot National Wildlife Refuge (Part of the West Tennessee National Wildlife Refuge Complex) | Lake County Obion County Fulton County, Kentucky | TN | 1941 | 10,428 acres (42.20 km$^2$) |
| Tennessee National Wildlife Refuge | Benton County Decatur County Henry County Humphreys County | TN | 1945 | 51,359 acres (207.84 km$^2$) |
| Aransas National Wildlife Refuge | Aransas County Calhoun County Refugio County | TX | 1937 | 114,657 acres (464.00 km$^2$) |
| Attwater Prairie Chicken National Wildlife Refuge | Colorado County | TX | 1972 | 10,528 acres (42.61 km$^2$) |
| Balcones Canyonlands National Wildlife Refuge | Burnet County Travis County Williamson County | TX | 1992 | 46,080 acres (186.5 km$^2$) |
| Big Boggy National Wildlife Refuge | Matagorda County | TX | 1983 | 5,000 acres (20 km$^2$) |
| Brazoria National Wildlife Refuge | Brazoria County | TX | 1969 | 44,414 acres (179.74 km$^2$) |
| Buffalo Lake National Wildlife Refuge | Randall County | TX | 1958 | 7,680 acres (31.1 km$^2$) |
| Caddo Lake National Wildlife Refuge | Harrison County | TX | 2000 | 8,493 acres (34.37 km$^2$) |
| Hagerman National Wildlife Refuge | Grayson County | TX | 1946 | 11,320 acres (45.8 km$^2$) |
| Jocelyn Nungaray National Wildlife Refuge (formerly Anahuac NWR) | Chambers County | TX | 1963 | 37,000 acres (150 km$^2$) |
| Laguna Atascosa National Wildlife Refuge | Cameron County Willacy County | TX | 1946 | 65,096 acres (263.43 km$^2$) |
| Lower Rio Grande Valley National Wildlife Refuge | Cameron County Hidalgo County Starr County Zapata County | TX | 1979 | 90,788 acres (367.41 km$^2$) |
| McFaddin National Wildlife Refuge | Jefferson County | TX | 1980 | 58,861 acres (238.20 km$^2$) |
| Moody National Wildlife Refuge | Chambers County | TX | | 3,517 acres (14.23 km$^2$) (Easement on private land) |
| Muleshoe National Wildlife Refuge | Bailey County | TX | 1935 | 6,440 acres (26.1 km$^2$) |
| Neches River National Wildlife Refuge | Anderson County Cherokee County | TX | 2006 | 4,404 acres (17.82 km$^2$) |
| San Bernard National Wildlife Refuge | Brazoria County Matagorda County | TX | 1969 | 45,730 acres (185.1 km$^2$) |
| Santa Ana National Wildlife Refuge | Hidalgo County | TX | 1943 | 2,088 acres (8.45 km$^2$) |
| Texas Point National Wildlife Refuge | Jefferson County | TX | 1979 | 8,972 acres (36.31 km$^2$) |
| Trinity River National Wildlife Refuge | Liberty County | TX | 1994 | 30,000 acres (120 km$^2$) |
| Bear River Migratory Bird Refuge | Box Elder County | UT | 1928 | 79,000 acres (320 km$^2$) |
| Fish Springs National Wildlife Refuge | Juab County | UT | 1959 | 17,992 acres (72.81 km$^2$) |
| Ouray National Wildlife Refuge | Uintah County | UT | 1960 | 11,987 acres (48.51 km$^2$) |
| Missisquoi National Wildlife Refuge | Franklin County | VT | 1943 | 6,642 acres (26.88 km$^2$) |
| Buck Island National Wildlife Refuge (Managed by the Caribbean Islands National Wildlife complex) | St. Thomas | VI | 1969 | 45 acres (0.18 km$^2$) |
| Green Cay National Wildlife Refuge (Managed by the Caribbean Islands National Wildlife complex) | St. Croix | VI | 1977 | 14 acres (0.057 km$^2$) |
| Sandy Point National Wildlife Refuge (Managed by the Caribbean Islands National Wildlife complex) | St. Croix | VI | 1984 | 360 acres (1.5 km$^2$) |
| Back Bay National Wildlife Refuge | Virginia Beach | VA | 1938 | 9,062 acres (36.67 km$^2$) |
| Chincoteague National Wildlife Refuge | Accomack County Worcester County, Maryland | VA | 1943 | 14,000 acres (57 km$^2$) |
| Eastern Shore of Virginia National Wildlife Refuge | Northampton County | VA | 1984 | 1,127 acres (4.56 km$^2$) |
| Elizabeth Hartwell Mason Neck National Wildlife Refuge (Part of the Potomac River National Wildlife Refuge Complex) | Fairfax County | VA | 1969 | 2,277 acres (9.21 km$^2$) |
| Featherstone National Wildlife Refuge (Part of the Potomac River National Wildlife Refuge Complex) | Prince William County | VA | 1970 | 325 acres (1.32 km$^2$) |
| Fisherman Island National Wildlife Refuge | Northampton County | VA | 1973 | 1,850 acres (7.5 km$^2$) |
| Great Dismal Swamp National Wildlife Refuge | Chesapeake Suffolk Camden County, North Carolina Gates County, North Carolina Pasquotank County, North Carolina | VA | 1974 | 112,000 acres (450 km$^2$) |
| James River National Wildlife Refuge (Part of the Eastern Virginia Rivers National Wildlife Refuge Complex) | Prince George County | VA | 1991 | 4,200 acres (17 km$^2$) |
| Nansemond National Wildlife Refuge | Suffolk | VA | 1973 | 411 acres (1.66 km$^2$) |
| Occoquan Bay National Wildlife Refuge (Part of the Potomac River National Wildlife Refuge Complex) | Prince William County | VA | 1998 | 644 acres (2.61 km$^2$) |
| Plum Tree Island National Wildlife Refuge^ | Poquoson | VA | 1972 | 3,501 acres (14.17 km$^2$) |
| Presquile National Wildlife Refuge (Part of the Eastern Virginia Rivers National Wildlife Refuge Complex) | Chesterfield County | VA | 1953 | 1,329 acres (5.38 km$^2$) |
| Rappahannock River Valley National Wildlife Refuge (Part of the Eastern Virginia Rivers National Wildlife Refuge Complex) | Caroline County Essex County King George County Richmond County | VA | 1996 | 9,030 acres (36.5 km$^2$) |
| Wallops Island National Wildlife Refuge | Wallops Island, Accomack County | VA | 1975 | 373 acres (1.51 km$^2$) |
| Billy Frank Jr. Nisqually National Wildlife Refuge | Thurston County Pierce County | WA | 1974 | 4,529 acres (18.33 km$^2$) |
| Columbia National Wildlife Refuge | Adams County Grant County | WA | 1944 | 29,596 acres (119.77 km$^2$) |
| Conboy Lake National Wildlife Refuge | Klickitat County | WA | 1964 | 7,072 acres (28.62 km$^2$) |
| Copalis National Wildlife Refuge (Part of the Washington Maritime National Wildlife Refuge Complex) | Grays Harbor County | WA | 1907 | 60.8 acres (0.246 km$^2$) |
| Dungeness National Wildlife Refuge (Part of the Washington Maritime National Wildlife Refuge Complex) | Clallam County | WA | 1915 | 772.52 acres (3.1263 km$^2$) |
| Flattery Rocks National Wildlife Refuge (Part of the Washington Maritime National Wildlife Refuge Complex) | Clallam County | WA | 1907 | 125 acres (0.51 km$^2$) |
| Franz Lake National Wildlife Refuge | Skamania County | WA | 1990 | 551.73 acres (2.2328 km$^2$) |
| Grays Harbor National Wildlife Refuge | Grays Harbor County | WA | 1990 | 1,471.38 acres (5.9545 km$^2$) |
| Julia Butler Hansen Refuge for the Columbian White-Tailed Deer | Wahkiakum County Clatsop County, Oregon Columbia County, Oregon | WA | 1972 | 5,600 acres (23 km$^2$) |
| Little Pend Oreille National Wildlife Refuge | Pend Oreille County Stevens County | WA | 1939 | 42,593.57 acres (172.3701 km$^2$) |
| McNary National Wildlife Refuge | Walla Walla County | WA | 1956 | 14,664.54 acres (59.3453 km$^2$) |
| Pierce National Wildlife Refuge | Skamania County | WA | 1983 | 329.38 acres (1.3330 km$^2$) |
| Protection Island National Wildlife Refuge (Part of the Washington Maritime National Wildlife Refuge Complex) | Jefferson County | WA | 1982 | 659.31 acres (2.6681 km$^2$) |
| Quillayute Needles National Wildlife Refuge (Part of the Washington Maritime National Wildlife Refuge Complex) | Clallam County Jefferson County | WA | 1907 | 300.2 acres (1.215 km$^2$) |
| Ridgefield National Wildlife Refuge | Clark County | WA | 1965 | 5,228.1 acres (21.157 km$^2$) |
| Saddle Mountain National Wildlife Refuge/Hanford Reach National Monument | Adams County Benton County Franklin County Grant County | WA | 2000 | 194,451 acres (786.92 km$^2$) |
| San Juan Islands National Wildlife Refuge (Part of the Washington Maritime National Wildlife Refuge Complex) | San Juan County | WA | 1976 | 454 acres (1.84 km$^2$) |
| Steigerwald Lake National Wildlife Refuge | Clark County | WA | 1987 | 1,049 acres (4.25 km$^2$) |
| Toppenish National Wildlife Refuge | Yakima County | WA | 1964 | 1,978 acres (8.00 km$^2$) |
| Turnbull National Wildlife Refuge | Spokane County | WA | 1937 | 18,684.98 acres (75.6154 km$^2$) |
| Umatilla National Wildlife Refuge | Benton County Morrow County, Oregon | WA | 1969 | 23,783 acres (96.25 km$^2$) |
| Willapa National Wildlife Refuge | Pacific County | WA | 1937 | 11,000 acres (45 km$^2$) |
| Canaan Valley National Wildlife Refuge | Tucker County | WV | 11 July 1994 | 16,613.44 acres (67.2322 km$^2$) |
| Ohio River Islands National Wildlife Refuge | Ohio River | WV | 1990 | 2,664.54 acres (10.7830 km$^2$) |
| Driftless Area National Wildlife Refuge | Grant County Richland County Illinois Iowa | WI | 1989 | 812 acres (3.29 km$^2$) |
| Fox River National Wildlife Refuge | Marquette County | WI | 1979 | 1,054 acres (4.27 km$^2$) |
| Gravel Island National Wildlife Refuge | Door County | WI | 1913 | 28 acres (0.11 km$^2$) |
| Green Bay National Wildlife Refuge | Door County, Delta County, Michigan | WI | 1912 | 330 acres (1.3 km$^2$) |
| Hackmatack National Wildlife Refuge | Walworth County | WI | 2012 | 11 acres (0.045 km$^2$) |
| Horicon National Wildlife Refuge | Dodge County Fond du Lac County | WI | 1941 | 21,400 acres (87 km$^2$) |
| Leopold Wetland Management District | Southeastern Wisconsin | WI | 1993 | 13,000 acres (53 km$^2$) |
| Necedah National Wildlife Refuge | Juneau County | WI | 1939 | 43,696 acres (176.83 km$^2$) |
| St. Croix Wetland Management District | West Central Wisconsin | WI | 1992 | 7,500 acres (30 km$^2$) |
| Trempealeau National Wildlife Refuge | Buffalo County Trempealeau County | WI | 1936 | 6,226 acres (25.20 km$^2$) |
| Whittlesey Creek National Wildlife Refuge | Bayfield County | WI | 1999 | 329 acres (1.33 km$^2$) |
| Bamforth National Wildlife Refuge | Albany County | WY | 1932 | 1,116 acres (4.52 km$^2$) |
| Cokeville Meadows National Wildlife Refuge | Lincoln County | WY | 1993 | 26,657 acres (107.88 km$^2$) |
| Hutton Lake National Wildlife Refuge | Albany County | WY | 1932 | 1,968 acres (7.96 km$^2$) |
| Mortenson Lake National Wildlife Refuge | Albany County | WY | 1993 | 1,776 acres (7.19 km$^2$) |
| National Elk Refuge | Teton County | WY | 1912 | 25,000 acres (100 km$^2$) |
| Pathfinder National Wildlife Refuge | Carbon County Natrona County | WY | 1909 | 16,807 acres (68.02 km$^2$) |
| Seedskadee National Wildlife Refuge | Sweetwater County | WY | 1965 | 27,230 acres (110.2 km$^2$) |
| Navassa Island National Wildlife Refuge (Part of the Caribbean Islands National Wildlife Refuge Complex) | | UM | | |
| Baker Island National Wildlife Refuge (Managed under the Hawaiian Islands National Wildlife Refuge) | | UM | | |
| Howland Island National Wildlife Refuge (Managed under the Hawaiian Islands National Wildlife Refuge) | | UM | | |
| Jarvis Island National Wildlife Refuge (Managed under the Hawaiian Islands National Wildlife Refuge) | | UM | | |
| Johnston Atoll National Wildlife Refuge (Managed under the Hawaiian Islands National Wildlife Refuge) | | UM | | |
| Kingman Reef National Wildlife Refuge (Managed under the Hawaiian Islands National Wildlife Refuge) | | UM | | |
| Palmyra Atoll National Wildlife Refuge (Managed under the Hawaiian Islands National Wildlife Refuge) | | UM | | |
| Wake Atoll National Wildlife Refuge (Managed under the Hawaiian Islands National Wildlife Refuge) | | UM | | |