# List of NFTA Metro bus routes
## Overview

NFTA Metro carried many of its present route numbers and names from the previous International Railway Company routes. Otherwise, much of the routing follows (loosely) the following number schematic:
* 1-49 City of Buffalo and Erie County routes
* 50-59 City of Niagara Falls and Niagara County routes
* 60-81 Express routes to/from Downtown Buffalo
* 90-97 Special event routes
* 101-118 School bus routes
* 200-216 Metrolink routes
The routes recently underwent a major revamping. Gone are the original "A" designations to "City Line". Replacing them are destinations to general areas as noted in the listings.

### Currently operating routes

| Route Number | Route Name | Originating Garage (1, 2) | Terminals | Notes |
| 1 Lovejoy 1A | William | BW | Downtown (Buffalo Metropolitan Transportation Center) City Line (Goethe Loop) Appletree (Appletree Business Park) | As of December 4, 2016, some trips have been adjusted to serve South Ogden Plaza. As of June 18, 2017, route no longer lays over at Carolina Street and Busti Avenue. |
| 2 Kaisertown 2A 2B | Clinton | BW | Downtown (Buffalo Metropolitan Transportation Center) City Line (Fernwood Loop) Transit (Flix Transit 10) Appletree (Appletree Business Park) | As of June 18, 2017, route no longer lays over at Carolina Street and Busti Avenue. As of February 13, 2022, service to Bank of America (Transit Road) discontinued. |
| 3 Riverside | Grant | Fr | Downtown (North Division Street and Ellicott Street) City Line (Vulcan Loop) | As of June 21, 2015, due to construction of the new Delaware North headquarters, resulting in safety concerns with West Chippewa Street being too narrow for buses to continue navigating, route will operate as follows: outbound, Niagara Square to Niagara Street, right Elmwood Avenue and continue regular route beyond West Chippewa Street. As of June 19, 2016, route no longer services Kenmore and Military; instead, it services Vulcan Loop, previously served by route 5 and 35 buses after those routes began servicing the new Black Rock/Riverside Transit Hub in December 2015. As of September 4, 2016, some trips have been added to serve Charter School for Applied Technologies. As of February 13, 2022, inbound route no longer services West Huron Street. |
| 4 Sloan 4B | Broadway | BW | Downtown (Buffalo Metropolitan Transportation Center) City Line (Michael Loop) Thruway Mall | "Sloan" trips operate to Michael Loop, "B" trips operate via Broadway to Harlem. As of June 18, 2017, route no longer lays over at Carolina Street and Busti Avenue. As of March 4, 2018, "H" trips discontinued. As of February 13, 2022, most weekend trips serve Thruway Mall. |
| 5 University Station | Niagara-Kenmore | Fr | Downtown (North Division Street and Ellicott Street) University Station | Route changed to move service over Sheridan Drive to separate route 35 and add service from previous route 30. As of December 6, 2015, route no longer services Hertel Avenue or Tonawanda Street between Hertel and Ontario, nor does it service Vulcan Loop. |
| 6 Galleria Mall | Sycamore | BW | Downtown (Lower Terrace and Charles Street) Walden Galleria Mall | Route changed to move service over Galleria Drive to allow easier access to Walden Galleria Mall; as such, route no longer services Walden Avenue east of Galleria Drive. As of February 13, 2022, route no longer services Waterfront Village; instead, route will operate as follows; inbound, regular route to Washington and North Division, right North Division, continue Church, left Lower Terrace, layover; outbound, Lower Terrace, left Seneca, left Franklin, right Church, continue South Division, left Ellicott, regular route. |
| 8 Main-Bailey/Waterfront Village | Main | CS | Marine Drive City Line (Main-Bailey Loop) | As of October 31, 2010, route no longer services Michigan Avenue. As of June 21, 2015, route will operate as follows: regular route to Wilkeson and Erie, right Erie, left Marine Drive and continue regular route. As of March 16, 2020, route changed to add weekend service to the Buffalo-Niagara Medical Campus to alleviate pressure off routes 14 and 16; as such, route no longer services Goodell Street; additionally, bus stops at Goodell Street and Main Street and Main Street and Virginia Street permanently discontinued. Instead, outbound route will operate as follows: regular route to Ellicott and Goodell, continue Ellicott, left Carlton, right Main, regular route. As of February 13, 2022, service to University Plaza reinstated. |
| 11A 11D | Colvin | Fr | Downtown (North Division Street and Ellicott Street) Paramount (Paramount Loop) Tonawanda Industrial Park (Fillmore Avenue and Wales Avenue) | As of March 6, 2016, route changed to move service over Hertel Avenue to alleviate pressure off route 23 due to safety concerns with the fleet of LFS CNG buses being too big to meet the 9'1" height restriction of the rail bridge that crosses over Colvin Avenue; as such, route no longer services Amherst Street or Colvin Avenue south of Hertel Avenue. Instead, route will operate as follows: inbound, regular route to Colvin and Hertel, right Hertel, left Delaware, regular route; outbound, regular route to Delaware and Amherst, continue Delaware, right Hertel, left Colvin, regular route. |
| 12 Niagara Street | Utica | CS | Niagara (Niagara Street and Hampshire Street) City Line (Main-Bailey Loop) | As of October 31, 2010, route no longer services Huntley Loop. As of June 21, 2015, route changed to move service over Richmond Avenue; due to safety concerns with Chenango Street and Hampshire Street between West Ferry Street and Winter Street being too narrow for buses to continue navigating, route no longer services Hampshire Street, Chenango Street or West Utica Street west of Richmond Avenue. As of February 13, 2022, route changed to alleviate pressure off routes 13 and 23; as such, route no longer services French Street or Kehr Street. Instead, route will operate as follows: inbound, regular route to East Ferry and Kehr, continue East Ferry, left Fillmore, right East Utica, regular route; outbound, regular route to East Utica and Fillmore, left Fillmore, right East Ferry, regular route. |
| 13 Utica Station 13C 13D 13E | Kensington | CS | Utica Station (Main Street and East Utica Street) University Station | As of October 30, 2010, "B" trips discontinued. 13C operates from Hutch Tech HS to University Station. |
| 14 Ridge Road 14 B 14 C | Abbott | BW | Downtown (Washington Street and Mohawk Street) Buffalo-Niagara Medical Campus (Washington Street and Carlton Street) The Shops McKinley Mall ECC South (Erie Community College South Campus) | As of June 21, 2015, due to construction of LECOM Harborcenter, resulting in safety concerns with Washington Street between Scott Street and Perry Street being too narrow for buses to continue navigating, route no longer services Perry Street west of Michigan Avenue or Washington Street south of Exchange Street. Instead, route will operate as follows: inbound, regular route to Michigan and Perry, continue Michigan, right Carroll, right North Carroll, right Exchange, right Washington and continue regular route; outbound, regular route to Washington and Exchange, left Exchange, left North Carroll, left Seneca, left Michigan and continue regular route. As of March 8, 2020, route no longer services Ellicott Street or Washington Street north of Genesee Street; instead, route will operate as follows: regular route to Ellicott and Genesee, right Genesee, left Michigan, left Carlton and layover; outbound, Carlton, right Main, right High, right Michigan, right Genesee, left Washington, regular route. As of April 4, 2022, due to North Carroll being permanently closed, route will operate as follows: Inbound, regular route to Michigan and Scott, left Scott, right Washington, continue regular route. Outbound, regular route to Washington and Scott Streets, left Scott, right Michigan. |
| 15 Cazenovia Park 15B | Seneca | BW | Downtown (Lower Terrace and Charles Street) City Line Southgate Plaza | As of June 2021, service resumes for some trips to start/end at City Line. All weekend and holiday service will continue "B" trip to Southgate Plaza. |
| 16 Botanical Gardens 16B 16H | South Park | BW | Downtown (Washington Street and Mohawk Street) Buffalo-Niagara Medical Campus (Washington Street and Carlton Street) City Line (Lackawanna Victory Transit Center) McKinley Mall Hamburg (Main Street and Buffalo Street) | As of June 21, 2015, due to construction of LECOM Harborcenter, resulting in safety concerns with Washington Street between Scott Street and Perry Street being too narrow for buses to continue navigating, route no longer services Perry Street west of Michigan Avenue or Washington Street south of Exchange Street. Instead, route will operate as follows: inbound, regular route to Michigan and Perry, continue Michigan, right Carroll, right North Carroll, right Exchange, right Washington and continue regular route; outbound, regular route to Washington and Exchange, left Exchange, left North Carroll, left Seneca, left Michigan and continue regular route. As of March 4, 2018, new "H" trips added to serve Hamburg due to discontinuation of route 36. As of March 8, 2020, route no longer services Ellicott Street or Washington Street north of Genesee Street; instead, route will operate as follows: regular route to Ellicott and Genesee, right Genesee, left Michigan, left Carlton and layover; outbound, regular route to Washington and Carlton, continue Carlton, right Main, right High, right Michigan, right Genesee, left Washington, regular route. As of April 4, 2022, due to North Carroll being permanently closed, route will operate as follows: Inbound, regular route to Michigan and Scott, left Scott, right Washington, continue regular route. Outbound, regular route to Washington and Scott Streets, left Scott, right Michigan. |
| 18 Delavan Station | Jefferson | CS | Delavan/Canisius University Station Louisiana (Louisiana Street and South Park Avenue) | As of June 19, 2016, route no longer services Louisiana Street between Seneca Street and Perry Street, nor does it service Seneca Street west of Hamburg Street; however, route extended to Larkinville, previously serviced by route 24. |
| 19 University Station | Bailey | BW | University Station Bailey/Abbott | A few trips operate over Dingens Street into New Buffalo Industrial Park. |
| 20 Orchard | Elmwood | Fr | Downtown (Ellicott Loop) Orchard (Orchard Loop) | As of June 21, 2015, due to construction of the new Delaware North headquarters, resulting in safety concerns with West Chippewa Street being too narrow for buses to continue navigating, route will operate as follows: Outbound, Niagara Square to Niagara Street, right Elmwood Avenue and continue regular route beyond West Chippewa Street. As of February 13, 2022, inbound route no longer services West Huron Street. |
| 22 West Side | Porter-Best | CS | Niagara Thruway Mall | Route extended to alleviate pressure off route 6; as such, route no longer services Walden-Bailey Loop. |
| 23A 23R 23S | Fillmore-Hertel | CS/Fr | Black Rock/Riverside Transit Hub Bailey/Abbott | Route changed to move service over Niagara Street to service the new Black Rock/Riverside Transit Hub. As of December 6, 2015, route no longer services the Marina Vista Apartments. 23R operates from Bailey Abbott Loop to Riverside HS. 23S operates from Mckinley HS to Bailey Abbott Loop. |
| 24A 24B 24L | Genesee | CS | Downtown (Waterfront Village) City Line (Andrews Loop) Airport (Buffalo-Niagara International Airport) | As of June 19, 2016, some trips have been adjusted to serve IQOR. As of February 13, 2022, route no longer services Exchange Street Station; instead, route changed to end at Waterfront Village, previously served by route 6 when that route was changed to end at Lower Terrace and Charles to alleviate pressure off route 15. |
| 24X | Genesee Airport/Downtown Express | CS | Downtown (Canalside station) Airport (Holtz Road Park and Ride) | As of June 17, 2019, route replaces portion of route 204 service to Buffalo Niagara International Airport. |
| 25A 25B 25D | Delaware | Fr | Downtown (Ellicott Loop) Orchard Tonawanda (Main and Niagara Transit Center) North Tonawanda (Mid City Plaza) | As of June 21, 2015, "D" trips extended to Mid City Plaza due to discontinuation of route 57. |
| 26A 26B | Delavan | CS | Niagara Thruway Mall | Route changed to move service over Harlem Road to replace route 24D trips to Thruway Mall. As such, "A" trips no longer service Andrews Loop. 26B "City Line" operates from Lafayette HS to Andrews Loop. 26B "Genesee" operates from McKinley HS to around Genesee and Best Streets. 26A "McKinley HS" operates from Andrews Loop to McKinley HS. 26B "Riverside HS" operates from Andrews Loop to Riverside HS. |
| 32 Black Rock Riverside Transit Hub 32B | Amherst | BW | Black Rock/Riverside Transit Hub Thruway Mall | Route changed to move service over Galleria Drive to allow easier access to Walden Galleria Mall; as such, route no longer services Walden Avenue between Galleria Drive and Millennium Hotel entrance. As of December 6, 2015, route changed to move service over Niagara Street to service the new Black Rock/Riverside Transit Hub; as such, route no longer services Tonawanda Street north of Amherst Street or Hamilton Street. 32B "Riverside HS" inbound operates from Huntley Loop to Riverside HS, while outbound operates from Riverside HS to Thruway Mall. |
| 34A 34B | Niagara Falls Blvd. | Fr | University Station Amherst Development Park Audubon (Hazelwood Drive and Woodridge Drive) | Most buses serve Boulevard Mall. As of September 6, 2010, "M" trips discontinued. As of September 19, 2016, bus stop at Niagara Falls Boulevard and Treadwell Road permanently discontinued, while bus stop previously north of mall entrance near T.G.I.Friday's has been permanently moved to north of the current Gabe's site. Outbound buses now enter the Boulevard Mall via Almeda Avenue near Chili's; inbound buses enter as usual, but turn right upon entering mall parking lot instead of left behind M&T Bank. As of March 8, 2020, weekend service permanently extended; last Saturday outbound bus leaves University Station at 10:30 pm, last Sunday inbound bus leaves Audubon at 9:43 pm, last outbound bus leaves University Station at 9:55 pm. As of December 27, 2020, "H" trips discontinued; also, route no longer services portion of Creekside Road between Niagara Falls Boulevard and Woodridge Drive or portion of North French Road between Sweet Home Road and Pineview Drive; outbound, regular route to Niagara Falls Boulevard and Creekside Drive, continue Niagara Falls Boulevard to East Robinson Road, right East Robinson, left Sundridge, right Glenhaven, right Sweet Home (where inbound buses currently travel heading to University Station), continue Sweet Home to Dodge Road, right Commerce, right Pineview, left Creekside, left John Glenn, right Commerce, right Hazelwood; inbound buses leave Audubon turning left onto Woodridge, left Commerce, left John Glenn, right Creekside, right Pineview, left Commerce, left Sweet Home, left Glenhaven, left Sundridge, right East Robinson, left Niagara Falls Boulevard (where outbound buses currently travel heading to Audubon Industrial Park), regular route. |
| 35A | Sheridan | Fr | Black Rock/Riverside Transit Hub Flint Circle (University at Buffalo, The State University of New York North Campus) | Route 35 service replaces service on previous 5 trips over Sheridan Drive, as well as portion of route 34M trip over Alberta Drive that was discontinued with the fall 2010 service changes. Service is extended to the University at Buffalo, The State University of New York North Campus. As of December 6, 2015, route no longer services Vulcan Loop; instead, it services the new Black Rock/Riverside Transit Hub; however, it does service the Marina Vista Apartments, previously served by route 23. As of February 13, 2022, route no longer services Maple Road east of Sweet Home Road, nor does it service Flint Road; instead, route operates as follows: outbound, regular route to Maple and Sweet Home, left Sweet Home, right Rensch, right Hadley, left Ausperger, layover at Coventry Loop; inbound, right Ausperger, right Hadley, left Rensch, left Sweet Home, right Maple, regular route. |
| 40 Buffalo Niagara Falls | Buffalo-Niagara Falls (via Grand Island) | Fr | Buffalo (Buffalo Metropolitan Transportation Center) Niagara Falls (Portage Road Transit Center) | As of December 6, 2015, route no longer services Buffalo Avenue; instead, all buses will be required to enter and exit the Niagara Scenic Parkway; however, it does service the new Black Rock/Riverside Transit Hub. As of December 5, 2021, route will have limited stops between Black Rock/Riverside Transit Hub and Niagara Street and Maryland Street. |
| 42 Southgate | Lackawanna | BW | Downtown (East Mohawk Street and Ellicott Street) Southgate Plaza | As of March 4, 2018, route extended to Downtown Buffalo due to discontinuation of route 36. As of April 4, 2022, due to North Carroll being permanently closed, route will operate as follows: Inbound, regular route to Michigan and Scott, left Scott, right Washington, continue regular route. Outbound, regular route to Washington and Scott Streets, left on Scott, right on Michigan. As of March 2, 2025, Perry St. between Michigan Ave. and Chicago St. will no longer be serviced. Instead, the 42 will proceed as follows: Scott St., Chicago, St. Perry St. Louisiana St. both directions. |
| 44A 44B | Lockport | CS | Buffalo (University Station) Crosspoint (Crosspoint Business Park) Lockport (Main Street and Locust Street) | |
| 46 Lancaster 46A | Lancaster | BW | Thruway Mall Thruway Mall-via Harlequin | Route replaces route 6 service east of the Walden Galleria Mall and operates as a one-way loop past Buffalo-Depew. As of April 20, 2016, route services new Walmart at Walden Avenue and Nagel Road, previous site of the Super Flea Market. As of February 13, 2022; service to Harlequin Books discontinued and service to Amazon Lancaster started. |
| 47C | Wehrle (formerly Youngs Road) | CS | University Station Transit (Eastern Hills Mall) | As of February 13, 2022, route extended to Eastern Hills Mall to alleviate pressure off route 48 and replace portion of route 24P over Transit Road; as such, route no longer services Main Street east of Harlem Road, nor does it service Erie Community College North Campus or Buffalo Niagara International Airport; instead, route changed to operate as follows: outbound, regular route to Main and Harlem, right Harlem, left Wehrle, right Youngs, left Lawrence Bell, left Earhart, right Wehrle, left Transit, layover; inbound, left Transit, right Wehrle, left Earhart, right Lawrence Bell, right Youngs, left Wehrle, right Harlem, left Main, regular route. |
| 48A 48C | Williamsville | CS | University Station Transit (Eastern Hills Mall) | Most weekday trips serve Erie Community College North Campus. |
| 49 | East Amherst (formerly Millard Suburban) | CS | University Station Transit (Eastern Hills Mall) | As of February 13, 2022, route no longer services Sheridan Drive east of Hopkins Road. Service to Eastern Hills Mall reinstated to alleviate pressure off route 48. |
| 50A 50B | Main-Niagara (formerly University) | Fr | Portage Road Transit Center Niagara Falls Transit Center (near Fashion Outlets) | Service to Lewiston discontinued as of December 5, 2021. |
| 52A | North End Circulator (formerly Hyde Park) | Fr | St Mary's Hospital Portage Road Transit Center | |
| 55A 55T | Pine Ave. | Fr | Portage Road Transit Center Niagara Falls Transit Center (Near Fashion Outlets) Aquarium of Niagara | Serves Downtown Niagara Falls. Route 55T service operates from mid-May through October. Route 55B service to Niagara Falls Transit Center (near Fashion Outlets) and Summit Business Park discontinued as of September 6, 2020 due to the impact of COVID-19 to NFTA operations. |
| 59 | Airport-NCCC | Fr | Niagara Falls Transit Center (near Fashion Outlets) NCCC | Service began December 5, 2021. |
| 60 | Niagara Falls | Fr | Buffalo (Buffalo Metropolitan Transportation Center) Niagara Falls Transit Center (Near Fashion Outlets) | |
| 61 | Tonawanda | Fr | Downtown (North Division Street and Ellicott Street) Tonawanda (Main Street and Niagara Street) | |
| 64 | Lockport | CS | Downtown (North Division Street and Ellicott Street) Lockport (Main Street and Locust Street) | |
| 66 A 66 B | Williamsville | CS | Downtown (Allen/Medical Campus Station) Transit (Millersport Highway and Transit Road) | Service extended to Millersport Highway and Transit Road (66B) due to discontinuation of route 65. |
| 69 | Lancaster | BW | Downtown (Lower Terrace and Charles Street) Lancaster (Broadway and Central Avenue) | Route 69A service to Alden (Broadway and Sullivan Avenue) discontinued as of September 6, 2020 due to the impact of COVID-19 to NFTA operations. As of June 19, 2022, route number changed from 69B to 69. |
| 70 | East Aurora | BW | Downtown (Lower Terrace and Charles Street) East Aurora (Fillmore Avenue and Riley Street) | Route 70A service through Elma (Clinton Street and Bowen Road) discontinued as of February 13, 2022 due to a bus driver shortage. As of June 19, 2022, route number changed from 70C to 70. |
| 72 | Orchard Park | BW | Downtown (Lower Terrace and Charles Street) Orchard Park (S Abbott Road and Armor Duells Road) | Route 72A service to ECC South (Erie Community College South Campus) discontinued as of February 13, 2022 due to a bus driver shortage. As of June 19, 2022, route 72 (number changed from 72P) also no longer services ECC South. |
| 74 A 74 B | Hamburg | BW | Downtown (North Division Street and Ellicott Street) Athol Springs Transit Center Hamburg (Main Street and Buffalo Street) | Route 74B service to North Boston (Herman Hill Road) discontinued as of February 13, 2022 due to a bus driver shortage. As of June 19, 2022, route 74B service operates to Hamburg (Main Street and Buffalo Street). |
| 76 | Lakeshore (formerly Lotus Bay) | BW | Downtown (North Division Street and Ellicott Street) Seneca One Stop | As of March 21, 2021, service extended from Seneca Bingo to Seneca One Stop. As of September 5, 2021, route number changed from 76S to 76. |
| 77 | Buffalo-Niagara Falls | Fr | Buffalo (Buffalo Metropolitan Transportation Center) Niagara Falls (Portage Road Transit Center) | Service began December 5, 2021. |
| 81 | Eastside | CS | Downtown (South Division Street and Washington Street) | |
| 101 S | North-South | BW | Lackawanna ("The Shops") and Botanical Gardens to McKinley Vocational High School | Buffalo Public School bus routes. Only operate on school days, but are open to general public use. As of September 4, 2022, all route numbers changed to 101S. |
| 101 S | North-South | BW | Hutchinson Central Technical High School to Bailey/Abbott | Buffalo Public School bus routes. Only operate on school days, but are open to general public use. As of September 4, 2022, all route numbers changed to 101S. |
| 101 S | North-South | BW | South Park High School to Utica Station | Buffalo Public School bus routes. Only operate on school days, but are open to general public use. As of September 4, 2022, all route numbers changed to 101S. |
| 102 S | Bailey-East | BW | Kaisertown to McKinley Vocational High School | Buffalo Public School bus route. Only operates on school days—and then only one, one-way trip—but is open to general public use. Route 102B service from McKinley Vocational High School and Burgard Vocational High School to Lackawanna Victory Transit Center discontinued as of February 13, 2022. As of September 4, 2022, route number changed from 102F to 102S. |
| 103 S | East-Suburban | BW | Lackawanna ("The Shops") to/from St. Mary's High School, Lancaster (Central & Broadway) | Catholic High School bus route. Only operates on school days, but is open to general public use. As of September 4, 2022, route number changed from 103A to 103S. |
| 104 S | South-Central | Fr | City Honors School to City Line (Starin & Kenmore) | Buffalo Public School bus route. Only operates on school days—and then only one, one-way trip—but is open to general public use. As of September 5, 2021, route number changed from 104F to 104. As of September 4, 2022, route number changed from 104 to 104S. |
| 106 S | South-Suburban | BW | Downtown (Buffalo Metropolitan Transportation Center) to/from Saint Francis High School, Athol Springs | Catholic High School bus route. Only operates on school days, but is open to general public use. As of September 4, 2022, route number changed from 106A to 106S. |
| 110 S | West-North | CS | Burgard Vocational High School to Black Rock/Riverside Transit Hub | Buffalo Public School bus route. Only operates on school days—and then only one, one-way trip—but is open to general public use. As of September 4, 2022, route number changed from 110A to 110S. |
| 111 S | South-Michigan | CS | Lovejoy & North Ogden to/from Buffalo Academy for Visual and Performing Arts | Buffalo Public School bus routes. Only operate on school days, but are open to general public use. Route 111B service from East Community High School to Amherst Station discontinued as of February 13, 2022. As of September 4, 2022, all route numbers changed to 111S. |
| 111 S | South-Michigan | CS | City Honors School to Lovejoy & North Ogden | Buffalo Public School bus routes. Only operate on school days, but are open to general public use. Route 111B service from East Community High School to Amherst Station discontinued as of February 13, 2022. As of September 4, 2022, all route numbers changed to 111S. |
* (1) Originating Garage-Terminal routes are based at one of three garages: BW=Babcock-William, CS=Cold Springs and Fr=Frontier
* (2) All weekend and holiday service originate out of Frontier

### Previously operating routes

| Route Number | Route Name | Operation from/to | Notes |
| 7 | Baynes-Richmond | Downtown (North Division Street and Ellicott Street) Forest (H.H. Richardson Complex) | Service discontinued May 24, 2020 due to the impact of COVID-19 to NFTA operations. |
| 9 | Parkside/Zoo | Downtown Delavan/Canisius University Station City Line (Coburg Loop & Kenmore) Brighton & Eggert ("B" trips) | Service originated as the 9-Kenmore streetcar route in the early 1900s. Service replaced in 1994 by an extension of routes 18 and 39 (latter route discontinued December 24, 1995). Route then briefly serviced by Bflo Transit, Inc. |
| 10 | West Utica | Downtown Utica Station Niagara & Ferry | Service originated as a streetcar route in the early 1900s. Service combined with route 12 East Utica and renamed Utica on June 23, 1985. |
| 17 | Central Terminal | Downtown (Hotels) Buffalo Central Terminal | Service continued until 1980s with some service on route 4C, operating Fillmore Avenue, Paderewski Drive and Memorial Drive, before returning onto Broadway. |
| 17 | Kensington-Suburban | | Service began June 23, 1985. Service discontinued March 27, 1994. Replacement service offered by route 32 on Union Road from Wehrle Drive to Walden Galleria Mall and Thruway Mall, route 49 on Hopkins Road, Sheridan Drive, Transit Road and Maple Road and route 47 on Wehrle Drive, Erie Community College North Campus and Aero Drive. |
| 20 E | Late Night Express | Downtown (Delaware & West Chippewa) Delavan/Canisius University Station | Short-lived shuttle service between Canisius College, Medaille College, Buffalo State College and the Chippewa Entertainment District. Service originally operated weekends, then reduced to Thursday nights only before being discontinued entirely due to low ridership and budget cuts. |
| 21 | Michigan/Forest | Downtown Main-Michigan (Cold Spring Garage) Delavan/Canisius University Station Forest & Niagara | Service discontinued March 24, 1993. Replacement service offered by route 8B on Michigan Avenue from Best Street to William Street to ease pressure off route 8A before being discontinued October 31, 2010 and partially replaced March 8, 2020 by part of routes 14 and 16. |
| 27 | Ridge Road | Downtown or Ford Plant Southgate Plaza Orchard Park East Aurora | Service discontinued December 1995. Replacement service partially offered by route 42. |
| 27 | Erie County Home | Airport (Buffalo Niagara International Airport) Wende (Erie County Home) | Replaced service previously offered by route 24C. Passengers were required to transfer between routes at the Buffalo Niagara International Airport. Service discontinued March 7, 2015 due to extremely low ridership. |
| 28 | Sheridan | Northtown Plaza Sheridan & Mill | Service discontinued early 1980s. Replacement service partially offered by route 49B from University Station. |
| 29 | Wohlers | Niagara (Maryland Street and Efner Street) Delavan/Canisius University Station | As of June 19, 2016, route changed to move service over Main Street. Due to construction of the new School of Medicine and Biomedical Sciences medical school that has since been completed, route no longer serviced Washington Street between High Street and Virginia Street. Inbound passengers who wished to access Allen/Medical Campus Station were required to deboard across from the station at Main and Allen Streets. Also, outbound trips no longer serviced Riley Street or Northampton Street. Service discontinued May 24, 2020 due to the impact of COVID-19 to NFTA operations. |
| 30 | Kenmore | Dupont (Sheridan Drive) Vulcan Loop University Station Airport (Buffalo Niagara International Airport) | Service discontinued October 31, 2010. Replacement service offered by route 5 from Vulcan Loop to University Station and route 47C from University Station to Buffalo Niagara International Airport. |
| 31 | Ogden | University Station Broadway/Ogden McKinley Circle Nason Loop or Bailey/Abbott Bethlehem Steel | Service discontinued March 27, 1994 and replaced by an extension of route 22. |
| 33 | Tonawanda/North Tonawanda | Main/Niagara Warner/Witmer (M or P) Ruie/Nash (S, T or W) Hackett/Hinds (J or K) Ellicott Creek/Frederick (F) The Summit (B or P) | Service discontinued in 1988 and replaced by routes 20 (20T) and 25 (25H, 25M). |
| 35 | Hamburg via Abbott | Downtown McKinley Mall ECC South Hamburg (Main & Buffalo) | Service discontinued and replaced by an extension of route 14. Service currently does not operate south of Erie Community College South Campus. |
| 36 | Hamburg | Downtown (East Mohawk Street and Ellicott Street) Hamburg (Main Street and Buffalo Street) McKinley Mall | Service discontinued March 4, 2018 and replaced by an extension of routes 16 and 42. |
| 37 | Hamburg via Camp | Downtown Ford Plant South Park & Scranton Hamburg (Main & Buffalo) Boston | Replacement service partially offered by route 74 during rush hours. |
| 38 | Buffalo/Angola | Downtown Angola | Service (via Angola) reassigned March 1993 to route 76. Remaining route discontinued December 24, 1995 due to cost-cutting measures by the NFTA. |
| 39 | Parker | University Station or Delavan/Canisius University Station Brighton & Eggert Northtown Plaza or Tonawanda (Ellicott Creek) | Service discontinued December 24, 1995. Route then briefly serviced by Bflo Transit, Inc. |
| 41 | Sheridan/Harlem | University Station Thruway Plaza Southgate Plaza (B) | Service discontinued upon the December 2001 schedule changes. |
| 42 | North Campus | University Station Flint Circle | Shuttle service briefly operated in the 1990s between University Station and Flint Circle via North Bailey Avenue and Maple Road. |
| 43 | Mall Shuttle | Thruway Plaza Appletree Business Park | Service began March 1993 as an extension of routes 4 and 6 to serve Walden Galleria Mall and Appletree Business Park from Thruway Mall. Service discontinued March 2001 and extended on route 4 to compensate for lost route. Service changed October 31, 2010 to discontinue route between Walden Galleria and Appletree Business Park with route 46 added to compensate portion of routes 4, 6B, 6C, 6D and 6F that were discontinued. |
| 46 | ECMC Shuttle | Delavan/Canisius University Station Erie County Medical Center (ECMC) | This route operated as a shuttle to ease pressure off route 13A into Erie County Medical Center (ECMC). |
| 51 | Buffalo Av Military Road | Downtown Niagara Falls Krull The Summit | Service merged with route 53. |
| 51 | Military North | The Summit Niagara Transit Center Niagara University | Service discontinued October 30, 2010 due to low ridership. |
| 53 | Niagara | Downtown Niagara Falls The Summit | Service previously part of route 50-Buffalo Avenue-University-Highland. Service rejoined with route 50 on December 23, 2007 with Niagara Street alignment maintained. |
| 54 | Military | Portage Road Transit Center Niagara Transit Center | Service discontinued May 24, 2020 due to the impact of COVID-19 to NFTA operations. |
| 56 | River Road | Kenmore & Military (Frontier Garage) Main & Niagara The Summit Niagara Transit Center | Service began March 24, 1993 as a continuation of buses operating from Frontier Station to Niagara Falls routes. Service discontinued October 30, 2010. |
| 57 | Flex Tonawandas | Main Street and Niagara Street Niagara Falls International Airport | Replaced service previously offered on routes 25C and 25D into North Tonawanda. Service discontinued June 19, 2015 due to extremely low ridership. |
| 58 | Lockport City | | |
| 58 | Lockport-Niagara Falls | | Service discontinued in 1988. |
| 62 | Parker | Downtown Ellicott Creek | Service discontinued April 29, 2012 due to low ridership. |
| 63 | Riverside | Downtown Kenmore & Military | Service discontinued April 29, 2012 due to low ridership. |
| 65 | Amherst | Downtown Buffalo Millersport & Transit | Service discontinued April 29, 2012 due to low ridership and partially replaced by route 66 to Millersport Highway and Transit Road. |
| 67 | Cleveland Hill | Downtown (Allen/Medical Campus Station) ECC North (Erie Community College North Campus) | Service discontinued February 13, 2022 due to low ridership. Replacement service partially offered by routes 12 and 32. |
| 68 | George Urban | Downtown (Canalside station) Airport (Buffalo Niagara International Airport) | Service discontinued May 24, 2020 due to the impact of COVID-19 to NFTA operations. |
| 71 | Holland | | Service began March 24, 1993. Service discontinued December 24, 1995. Replacement service partially offered to Holland by routes 70 and 75 (latter route discontinued September 6, 2020 due to the impact of COVID-19 to NFTA operations). |
| 73 | Lancaster | | Service began March 24, 1993. Service discontinued December 24, 1995. Replacement service partially offered to Lancaster by route 69. |
| 75 | West Seneca | Downtown (Lower Terrace and Charles Street) Bank of America (Transit Road) Bullis (Seneca Creek Road and Union Road) | Service discontinued September 6, 2020 due to the impact of COVID-19 to NFTA operations. |
| 79 | Tonawanda | Downtown (Exchange Street Station) Main & Niagara | Service discontinued May 24, 2020 due to the impact of COVID-19 to NFTA operations. |
| 80 | ECC Shuttle | Tri-campus shuttle connecting ECC campuses | Service replaced by route 211 ECC Circulator (route 211 discontinued summer 2013 when route operation given to Erie Community College). |
| 82 | Outer Harbor Shuttle | Short-lived route serving businesses on the outer harbor. | Service discontinued in 2002 due to low ridership. |
| 90 | Bills Express Portage Road Transportation Center | Short-lived route serving Buffalo Bills home games at Highmark Stadium. | Service discontinued April 29, 2012 due to funding crisis. |
| 91 | Bills Express Tonawanda (Township) | Short-lived route serving Buffalo Bills home games at Highmark Stadium. | Service discontinued April 29, 2012 due to funding crisis. |
| 92 | Bills Express University Station | Short-lived route serving Buffalo Bills home games at Highmark Stadium. | Service discontinued April 29, 2012 due to funding crisis. |
| 93 | Bills Express Buffalo Metropolitan Transportation Center | Short-lived route serving Buffalo Bills home games at Highmark Stadium. | Service discontinued April 29, 2012 due to funding crisis. |
| 94 | Bills Express Niagara Falls Transportation Center | Short-lived route serving Buffalo Bills home games at Highmark Stadium. | Service discontinued April 29, 2012 due to funding crisis. |
| 95 | Bills Express Thruway Plaza | Short-lived route serving Buffalo Bills home games at Highmark Stadium. | Service discontinued April 29, 2012 due to funding crisis. |
| 96 | Bills Express Clarence Mall | Short-lived route serving Buffalo Bills home games at Highmark Stadium. | Service discontinued April 29, 2012 due to funding crisis. |
| 97 | Darien Lake | Service operated between Downtown Buffalo and Darien Lake Theme Park Resort. | Service discontinued April 29, 2012 due to low ridership and high cost of operating, although service was reinstated to run during the summer of 2012 as the season was about to begin when service was discontinued. |
| 112 | Grant-North | East High School to City Line (Kenmore & Vulcan) | Buffalo Public School bus route. Only operates on school days—and then only one, one-way trip—but is open to general public use. Service discontinued December 21, 2019. |
| 200 | North Tonawanda-Wheatfield | | Service discontinued May 1, 2012 due to low ridership. |
| 201 | Lockport Shuttle | Lockport (Main Street and Locust Street) | Hourly loop service in the City of Lockport. Service discontinued December 2, 2012 when route operation given to Niagara Rural Transportation as their route 2. |
| 202 | Metrolink Shuttle | Canalside station Parking Area (Michigan & Perry) | Short-lived shuttle route to parking area in lower Main Street area. Service discontinued March 25, 2007. |
| 203 | Erie County Home Express | Erie County Medical Center (ECMC) Wende | Shuttle service timed during shift changes for Erie County Home. Service discontinued October 30, 2010 and replaced by route 27, which was itself discontinued March 7, 2015 due to extremely low ridership. |
| 204 | Airport/Downtown Express | Downtown (North Division Street and Ellicott Street) Airport (Holtz Road Park and Ride) | Service discontinued June 17, 2019 and replaced by route 24X. |
| 206 | Buffalo State Circulator | Fall/Spring semester shuttle service for Buffalo State College students with additional service to Tops and Wegmans. | Service discontinued September 2, 2018. |
| 207 | Elmwood Circulator | Buffalo State College Buffalo Grand Hotel | Shuttle service for Buffalo State College students to shopping and entertainment. Service discontinued December 2010 due to route duplication on route 20. |
| 208 | Grant Circulator | Buffalo State College Grant & Ferry | Shuttle service for Buffalo State College students to shopping and entertainment. Service discontinued June 2, 2009 due to route duplication on route 3. |
| 209 | Medaille Circulator | Medaille College/Buffalo Zoo | Shuttle service connecting Medaille College and remote parking at Buffalo Zoo. Service discontinued May 1, 2012 due to low ridership and funding. |
| 210 | Airport-Niagara Falls Express | Service operated between Buffalo Niagara International Airport, Niagara Falls Transportation Center and Portage Road Transit Center. | Service discontinued April 29, 2012 due to low ridership and funding. |
| 211 | ECC Circulator | Fall/Spring semester tri-campus shuttle serving ECC City Campus to either ECC North or ECC South. | Service discontinued summer 2013 when route operation given to Erie Community College. |
| 216 | McKinley Mall-Gowanda | | Service discontinued May 1, 2012 due to low ridership and high cost of operating. |