# List of museums in Florida

This article lists museums currently operating across the U.S. state of Florida, together with summaries of their locations and main focuses. There are additional lists of Florida's defunct and proposed museums.

## Museums

| Museum Name | Image | Community | County | Region | Type | Notes |
| A. E. Backus Gallery & Museum | | Fort Pierce | St. Lucie | Central East | Art | Features landscapes by A. E. Backus and pieces by other Florida artists |
| A. Quinn Jones Museum and Cultural Center | | Gainesville | Alachua | North Central | History | Former home of A. Quinn Jones |
| ACCORD Civil Rights Museum | | St. Augustine | St. Johns | Northeast | Ethnic-African American | Former dental office of Dr.Robert Hayling, which also served as a meetinghouse for the St. Augustine Movement. Currently a private residence. |
| African-American Research Library and Cultural Center | | Fort Lauderdale | Broward | Southeast | Haitian art | Branch of the Broward County Library |
| African American Museum of the Arts | | DeLand | Volusia | Central East | Art | Art and culture of African Americans and Caribbean Americans |
| Ah-Tah-Thi-Ki Museum | | Clewiston | Hendry | Southwest | Ethnic-Native American | Managed by the Seminole Tribe of Florida at the Big Cypress Indian Reservation, artifacts, culture, and life of the Seminole in the swamps of Florida |
| Air Force Armament Museum | | Eglin Air Force Base | Okaloosa | Northwest | Aerospace | Air Force planes, bombs, missiles, rockets, and other military weapons |
| Air Force Space and Missile Museum | | Cape Canaveral | Brevard | Central East | Aerospace | Houses artifacts from early space program and includes extensive outdoor rocket garden with vintage missiles and rockets. |
| Airport Museum | | Melbourne | Brevard | Central East | Aviation | Houses displays on Naval Air Station Melbourne and Melbourne International Airport. |
| Albin Polasek Museum and Sculpture Gardens | | Winter Park | Orange | Central | Art | Sculptures of Albin Polasek and others, and changing art exhibits |
| Alexander Brest Museum and Gallery | | Jacksonville | Duval | Northeast | Art | Part of Jacksonville University, includes changing exhibits of contemporary art, collections of carved ivory, Pre-Columbian artifacts, Steuben glass, Chinese porcelain, Cloisonné, Tiffany glass, and Boehm porcelain |
| Alfred B. Maclay Gardens State Park | | Tallahassee | Leon | North Central | Historic house | 28 acres (110,000 m$^2$) of ornamental gardens, 1920s-1940s period Maclay House |
| Amelia Island Light | | Fernandina Beach | Nassau | Northeast | Maritime | |
| Amelia Island Museum of History | | Fernandina Beach | Nassau | Northeast | Local history | Housed in a former jail |
| American Museum of the Cuban Diaspora | | Miami | Miami-Dade | Southwest | Ethnic-Cuban American | |
| American Muscle Car Museum | | Melbourne | Brevard | Central East | Automotive | Not open to the public except for charitable events |
| American Police Hall of Fame & Museum | | Titusville | Brevard | Central East | Law enforcement | Police weapons, vehicles, equipment, badges, uniforms, procedures and more |
| American Victory Ship and Museum | | Tampa | Hillsborough | Central West | Maritime | World War II cargo ship |
| American Water Ski Educational Foundation Hall of Fame and Museum | | Polk City | Polk | Central | Sports | Also known as Water Ski Hall of Fame, features water ski and boating memorabilia |
| Anita S. Wooten Gallery | | Orlando | Orange | Central | Art | website, part of the Valencia College, East Campus |
| Anna Lamar Switzer Center for Visual Arts | | Pensacola | Escambia | Northwest | Art | website, contemporary art exhibits, part of Pensacola State College |
| Ann Norton Sculpture Gardens | | West Palm Beach | Palm Beach | Southeast | Art | Artist's home, studio and outdoor sculpture gardens |
| Anna Maria Island Museum and Historical Park | | Anna Maria Island | Manatee | Southwest | Local history | website |
| Apalachicola Maritime Museum | | Apalachicola | Franklin | Northwest | Maritime | Area maritime heritage, boat building, boat tours, 57-foot ketch |
| Appleton Museum of Art | | Ocala | Marion | Central | Art | Part of College of Central Florida, collections include sculptures, 19th-century paintings, Pre-Columbian, African, European and Asian arts and antiquities |
| Arcadia Mill | | Milton | Santa Rosa | Northwest | History | Remains of an early 19th-century industrial water-powered mill complex |
| Arch Creek Park | | North Miami Beach | Miami-Dade | Southeast | History | 8-acre park with museum containing Native American and pioneer artifacts, natural history programs |
| Archer Historical Society Museum | | Archer | Alachua | North Central | Local history | Housed in a railroad depot |
| Armory Art Center | | West Palm Beach | Palm Beach | Southeast | Art | website, community art center with exhibit gallery |
| Art and Culture Center of Hollywood | | Hollywood | Broward | Southeast | Art | Contemporary visual and performing arts |
| Art Center Sarasota | | Sarasota | Sarasota | Southwest | Art | Visual arts center with exhibit galleries and a sculpture garden |
| ArtCenter Manatee | | Bradenton | Manatee | Southwest | Art | website |
| Arts and Design Society of Fort Walton Beach | | Fort Walton Beach | Okaloosa | Northwest | Art | website, visual arts center with exhibit gallery |
| Art League of Daytona Beach Gallery | | Daytona Beach | Volusia | Central East | Art | Exhibits by local artists |
| ArtCenter/South Florida | | Miami Beach | Miami-Dade | Southeast | Art | website, gallery |
| Atlantic Center for the Arts | | New Smyrna Beach | Volusia | Central East | Art | Includes galleries in two locations |
| Audubon House and Tropical Gardens | | Key West | Monroe | Florida Keys | Historic house | 1830s period home of artist and ornithologist John James Audubon, includes gallery of his art |
| Bailey-Matthews Shell Museum | | Sanibel | Lee | Southwest | Natural history | Shells and mollusks |
| Baker Block Museum | | Baker | Okaloosa | Northwest | Local history | Complex includes the museum with antiques, tools and period displays, a post office, mill, log cabin, blacksmith shop, corn crib, and outhouse |
| Barbara Sumwalt Museum | | Bokeelia | Lee | Southwest | Local history | Operated by the Useppa Island Historical Society |
| Barnacle Historic State Park | | Coconut Grove | Miami-Dade | Southeast | Historic house | Includes 19th century period home and boathouse of yacht designer Ralph Middleton Munroe |
| Bass Museum of Art | | Miami Beach | Miami-Dade | Southeast | Art | Collections include Renaissance and Baroque paintings, Rococo court painting and English portraiture, painting and sculpture of North America, 19th and 20th century landscape and history paintings, painting and sculpture of North America, Latin America and the Caribbean, contemporary photography, Asian art, European decorative art |
| Beaches Museum and History Park | | Jacksonville | Duval | Northeast | Local history | Operated by the Beaches Area Historical Society, history and culture of the Jacksonville Beaches area |
| Bienes Museum of the Modern Book | | Fort Lauderdale | Broward | Southeast | Library | Changing exhibits from its collections |
| Bill Baggs Cape Florida State Park | | Key Biscayne | Miami-Dade | Southeast | Maritime | Tours of Cape Florida Lighthouse and keeper's quarters |
| Black Heritage Museum | | Middleburg | Clay | Northeast | Ethnic-African American | Open by appointment |
| Black Heritage Museum | | New Smyrna Beach | Volusia | Central East | Ethnic-African American | |
| Blanchard House Museum | | Charlotte Harbor | Charlotte | Southwest | Ethnic-African American | Area African American history and culture |
| Bob Rauschenberg Gallery | | Fort Myers | Lee | Southwest | Art | Modern and contemporary international art in all media, part of Florida SouthWestern State College, Lee County Campus |
| Boca Raton Children's Museum | | Boca Raton | Palm Beach | Southeast | Children's | Hands-on exhibits and exploration areas for young children |
| Boca Express Train Museum | | Boca Raton | Palm Beach | Southeast | Railroad | Includes restored rail cars & a steam engine |
| Boca Raton History Museum | | Boca Raton | Palm Beach | Southeast | Local history | Located in former Town Hall, operated by the Boca Raton Historical Society |
| Boca Raton Museum of Art | | Boca Raton | Palm Beach | Southeast | Art | Collections include 19th and 20th century European and American painting, drawings and sculpture; West African and Pre-Columbian art; modern and contemporary art; decorative arts and sculpture |
| Bok Tower Gardens | | Lake Wales | Polk | Central | Historic house | 1930s period mansion, carillon and botanical gardens |
| Bonnet House Museum & Gardens | | Fort Lauderdale | Broward | Southeast | Historic house | 20th century estate with art exhibits and gardens |
| Eastern Florida State College Planetarium & Observatory | | Cocoa | Brevard | Central East | Science | Exhibits are at Science Quest Exhibit Hall at Eastern Florida State College, also planetarium (temporarily closed), observatory and art exhibits. |
| Brevard Museum of History & Natural Science | | Cocoa | Brevard | Central East | Multiple | Natural history, science, local history and culture, operated by Florida Historical Society. |
| Bronson-Mulholland House | | Palatka | Putnam | Northeast | Historic house | Mid 19th-century mansion, operated by the Putnam County Historical Society |
| Brooksville Railroad Depot Museum | | Brooksville | Hernando | Central West | Railroad | Includes local history |
| Browning Railroad Museum | | Palatka | Putnam | Northeast | Railroad | Operated by the Palatka Railroad Preservation Society in the Palatka Railroad Depot, includes model train layout |
| Bulow Plantation Ruins Historic State Park | | Flagler Beach | Flagler | Northeast | History | Plantation ruins and exhibits |
| Burroughs Home | | Fort Myers | Lee | Southwest | Historic house | Turn-of-the-20th-century period house, open once a week for living history tours |
| Butler House Museum | | Deerfield Beach | Broward | Southeast | Historic house | Operated by the Deerfield Beach Historical Society |
| Butterfly World | | Coconut Creek | Broward | Southeast | Natural history | Live butterflies, insects and birds, gardens, museum display of mounted butterflies and insects |
| Cade Museum for Creativity and Invention | | Gainesville | Alachua | North Central | Science | Hands-on experiments and activities related to STEAM; exhibits about inventors and their inventions. |
| Calusa Nature Center and Planetarium | | Fort Myers | Lee | Southwest | Natural history | Includes live animals, programs and a planetarium |
| Camp Blanding Museum and Memorial Park | | Starke | Clay | Northeast | Military | |
| Camp Gordon Johnston Museum | | Carrabelle | Franklin | Northwest | Military | History of Camp Gordon Johnston, a WWII amphibious training base, includes artifacts, vehicles, photos, memorabilia |
| Cape Coral Historical Museum | | Cape Coral | Lee | Southwest | Local history | website, operated by the Cape Coral Historical Society |
| Cape St. George Light | | Apalachicola | Franklin | Northwest | Maritime | Reconstructed lighthouse to climb and keepers house |
| Casa Feliz Historic Home Museum | | Winter Park | Orange | Central | Historic house | 1930s Spanish-style estate designed by James Gamble Rogers II |
| Carrabelle History Museum | | Carrabelle | Franklin | Northwest | History | History and culture of Carrabelle, includes Native American artifacts and the original World's Smallest Police Station |
| CAS Gallery | | Coral Gables | Miami-Dade | Southeast | Art | website, part of University of Miami |
| Cason Cottage | | Delray Beach | Palm Beach | Southeast | Historic house | Operated by the Delray Beach Historical Society, reflects Florida's lifestyles from 1915 to 1935 |
| Castillo de San Marcos | | St. Augustine | St. Johns | Northeast | Military | Spanish fort and museum, living history re-enactments |
| Cedar Key Historical Museum | | Cedar Key | Levy | North Central | Local history | |
| Cedar Key Museum State Park | | Cedar Key | Levy | North Central | Historic house | 1920s period house with collection of shells and Native American artifacts |
| Central Florida Railroad Museum | | Winter Garden | Orange | Central | Railroad | |
| Centre Gallery | | Tampa | Hillsborough | Central West | Art | website, student-run gallery in the Marshall Center at the Wolfson campus, University of South Florida |
| Charlotte County Historical Center | | Charlotte Harbor | Charlotte | Southwest | Local history | website, located at Bayshore Live Oak Park, county history and culture |
| Child of the Sun Visitor Center | | Lakeland | Polk | Central | Architecture | Photographs, furniture, and drawings depicting Frank Lloyd Wright's relationship with Florida Southern College, also Wright exhibits |
| Children's Museum of the Highlands | | Sebring | Highlands | Central | Children's | website, interactive play experiences |
| Children's Science Explorium | | Boca Raton | Palm Beach | Southeast | Science | website, located in Sugar Sand Park |
| Clay County Historical Society Museum | | Green Cove Springs | Clay | Northeast | Local history | Located in an old depot, also railroad memorabilia |
| Clewiston Museum | | Clewiston | Hendry | Southwest | Local history | Houses exhibits highlighting the local Clewiston area, including the cattle industry, commercial fishing & Seminole Indians. |
| Cocoa Beach Surf Museum | | Cocoa Beach | Brevard | Central | History | Located in Ron Jon Surf Shop. |
| Collection on Palmetto | | Clearwater | Pinellas | Central West | Automotive | Museum of antique automobiles and engines |
| Coral Castle | | Homestead | Miami-Dade | Southeast | Art | Stone sculpture castle structure and garden |
| Corporal Larry E. Smedley National Vietnam War Museum | | Orlando | Orange | Central | Military | Vietnam War artifacts, equipment, photos |
| Coastal Heritage Museum | | Crystal River | Citrus | Central West | Local history | Located in the historic old city hall |
| Colonial Quarter | | St. Augustine | St. Johns | Northeast | Living | Life of Spanish soldiers and their families in 1740 St. Augustine |
| Conrad Center | | DeLand | Volusia | Central East | Local history | Operated by the West Volusia Historical Society, part of a complex that also includes the Henry A. DeLand House Museum and Lue Gim Gong Memorial Garden |
| Constitution Convention Museum State Park | | Port St. Joe | Gulf | Northwest | History | Recreated hall with life-size audio-animated figures telling the story of the first Constitution Convention of Florida, also exhibits of 19th century life |
| Coral Gables Merrick House | | Coral Gables | Miami-Dade | Southeast | Historic house | 1925 period home of the founder of Coral Gables |
| Coral Springs Museum of Art | | Coral Springs | Broward | Southeast | Art | Focus is present day, nationally recognized and Florida artists who create traditional, modern and Postmodern art |
| Cornell Fine Arts Museum | | Winter Park | Orange | Central | Art | Part of Rollins College, collection includes art and objects from antiquity to the 21st century |
| Cornell Museum of Art & American Culture | | Delray Beach | Palm Beach | Southeast | Art | Located in the Old School Square Cultural Arts Center, exhibits of fine art, craft and American culture |
| Cracker Country | | Tampa | Hillsborough | Central West | Living | Late 19th century rural village |
| Cracker Trail Museum | | Zolfo Springs | Hardee | Central | History | Located along the original Florida Cracker Trail, Florida pioneer history |
| Crane Point Museum, Nature Center & Historic Site | | Marathon | Monroe | Florida Keys | Natural history | Includes museum of natural history, children's museum, bird rehabilitation center |
| Crealdé School of Art | | Winter Park | Orange | Central | Art | Features three galleries: Alice & William Jenkins Gallery, Showalter Hughes Community Gallery, & Contemporary Sculpture Garden |
| Crooked River Lighthouse | | Carrabelle | Franklin | Northwest | Maritime | Late 19th-century lighthouse to climb, Keeper's House Museum |
| Crowley Natural and Cultural History Center | | Sarasota | Sarasota | Southwest | Multiple | website, includes museum of pioneer history, pioneer cabin, blacksmith shop, working sugar cane mill, restored 1892-period Tatum House, nature trails and boardwalk |
| Crystal River Archaeological State Park | | Crystal River | Citrus | Central West | Archaeology | Site includes 6 pre-Columbian mounds and museum with excavated finds |
| Cultural Center at Ponte Vedra Beach | | Ponte Vedra Beach | St. Johns | Northeast | Art | Exhibitions of local, regional and national artists in a variety of media |
| Cummer Museum of Art and Gardens | | Jacksonville | Duval | Northeast | Art | Collection includes European and American paintings, Meissen porcelain |
| Dade Battlefield Historic State Park | | Bushnell | Sumter | Central | Military | Site of the 1835 Second Seminole War battlefield, visitor center museum about the war |
| Dante Fascell Visitor Center | | Homestead | Miami-Dade | Southeast | Natural history | One of several visitor centers for Biscayne National Park, focus is the four ecosystems found in the park, also art gallery |
| Daytona 500 Experience | | Daytona Beach | Volusia | Central East | Automotive | |
| De Soto National Memorial | | Bradenton | Manatee | Southwest | History | Commemorates the 1539 landing of Hernando de Soto |
| DeBary Hall Historic Site | | DeBary | Volusia | Central East | Historic house | 10-acre (40,000 m$^2$) late 19th century estate with outbuildings |
| Deering Estate at Cutler | | Miami | Miami-Dade | Southeast | Multiple | Historic house, art exhibits, nature tours of its 444 acres (1.80 km$^2$) |
| DeLand House Museum | | DeLand | Volusia | Central East | Historic house | Victoria period house with artifacts, operated by the West Volusia Historical Society, complex includes the Conrad Center |
| DeLand Memorial Hospital Museum | | DeLand | Volusia | Central East | Multiple | Exhibits include 1920s operating room & apothecary, military memorabilia, early days of the area's ice and electric business, elephant-themed collectibles, toys and the West Volusia Black Heritage Gallery |
| DeLand Naval Air Station Museum | | DeLand | Volusia | Central East | Military | Military aircraft & artifacts |
| Depot Museum | | Avon Park | Highlands | Central | Local history | Housed in 1926 railroad depot, operated by the Historical Society of Avon Park |
| Destin History & Fishing Museum | | Destin | Okaloosa | Northwest | Multiple | Maritime and local history |
| Dixie Cultural Center | | Old Town | Dixie | North Central | Local history | |
| Don Garlits Museum of Drag Racing | | Ocala | Marion | Central | Automotive | History of the sport of drag racing |
| Dr. Carter G. Woodson African American History Museum | | St. Petersburg | Pinellas | Central West | Ethnic-African American | African American history and culture |
| Dudley Farm Historic State Park | | Newberry | Alachua | North Central | Farm | Working farm showing agricultural development in Florida from the 1850s through the mid-1940s |
| Duncan Gallery of Art | | DeLand | Volusia | Central East | Art | Part of Stetson University, features works by Southeast and student artists |
| Dundee Depot Museum | | Dundee | Polk | Central | Local history | Located in an old depot, also railroad memorabilia |
| Dunedin Fine Art Center | | Dunedin | Pinellas | Central West | Art | Art center with galleries and David L. Mason Children's Art Museum |
| Dunedin History Museum | | Dunedin | Pinellas | Central West | Local history | Local and state history and culture |
| Eden Gardens State Park | | Freeport | Walton | Northwest | Historic house | Includes the Wesley House with late 18th century furniture and gardens |
| Edison and Ford Winter Estates | | Fort Myers | Lee | Southwest | Multiple | Winter estates of Thomas Edison and Henry Ford, museum with their scientific inventions, and a botanical garden |
| Electrotherapy Museum | | West Palm Beach | Palm Beach | Southeast | Technology | Open by appointment, private collection includes static electric machines, galvanic and faradic batteries, Oudin resonators, Tesla coils, violet rays, diathermy machines and more |
| Elliott Museum | | Hutchinson Island | Martin | Central East | Multiple | American antiques, decorative arts, baseball memorabilia, vintage automobiles, technology and inventions |
| Emerald Coast Science Center | | Fort Walton Beach | Okaloosa | Northwest | Science | Themes include human senses, energy efficiency, color and light, animals, bubbles |
| Ernest Hemingway Home and Museum | | Key West | Monroe | Florida Keys | Historic house | 1930s residence of author Ernest Hemingway |
| Eugene L. Matthews Bradford County Historical Museum | | Starke | Bradford | Northeast | Local history | |
| Eustis Historical Museum | | Eustis | Lake | Central | Local history | Each room features a different theme with period antiques, also Citrus Museum |
| Excelsior Museum and Cultural Center | | St. Augustine | St. Johns | Northeast | Ethnic-African American | Located in St. Augustine's first black public high school, area African American history |
| Explorations V Children's Museum | | Lakeland | Polk | Central | Children's | Interactive exhibits on local history, science, agriculture, art |
| Fairchild Tropical Botanic Garden | | Coral Gables | Miami-Dade | Southeast | Multiple | Botanic garden, butterfly conservatory, art exhibits |
| Family Heritage House Museum | | Bradenton | Manatee | Southwest | African American | Part of State College of Florida, Manatee–Sarasota, gallery and resource center for the study of African-American achievements. |
| Fantasy of Flight | | Polk City | Polk | Central | Aviation | Formerly the Weeks Air Museum, which was located in Miami |
| Father Miguel O'Reilly House Museum | | St. Augustine | St. Johns | Northeast | Religious | Catholic heritage of St. Augustine |
| Flagler Beach Historical Museum | | Flagler Beach | Flagler | Northeast | Local history | Photos, documents, memorabilia, prehistoric bones, shells, Native American artifacts |
| Flagler Museum | | Palm Beach | Palm Beach | Southeast | Historic house | 55 room Gilded Age mansion, also known as Whitehall |
| Flamingo Gardens | | Davie | Broward | Southeast | Historic house | Botanical gardens and wildlife park with 1930s period Wray Home |
| Floral City Heritage Hall Museum | | Floral City | Citrus | Central West | Local history | Operated by the Floral City Heritage Council |
| Florida Agricultural Museum | | Palm Coast | Flagler | Northeast | Agriculture | Includes restored 1890s pioneer homestead, a turn of the last century dry goods store, five restored buildings from a 1930s Depression-Era citrus operation, and a 5,000-square-foot (460 m$^2$) dairy barn |
| Florida Air Museum | | Lakeland | Polk | Central | Aviation | Includes one-of-a-kind designs, classics, ultra-lights, antique airplanes and warbirds |
| Florida Atlantic University, University Galleries | | Boca Raton | Palm Beach | Southeast | Art | website, Schmidt Center Gallery and Ritter Art Gallery |
| Florida Carriage Museum & Resort | | Weirsdale | Marion | Central | Transportation | Collection of 160 carriages, and equine-related artifacts and artwork |
| Florida Civilian Conservation Corps Museum | | Sebring | Highlands | Central | History | Part of Highlands Hammock State Park, CCC activities in Florida |
| Florida Governor's Mansion | | Tallahassee | Leon | North Central | Historic house | Tours by appointment |
| Historic Train Depot Museum | | Tarpon Springs | Pinellas | Central West | History | Former railroad depot, exhibits on local history and the railroad |
| Florida Holocaust Museum | | St. Petersburg | Pinellas | Central West | Ethnic-Jewish | |
| Florida Keys Eco-Discovery Center | | Key West | Monroe | Florida Keys | Natural history | Plants and animals of the Florida Keys National Marine Sanctuary |
| Florida Maritime Museum at Cortez | | Cortez | Manatee | Southwest | Maritime | Includes a shell collection, ship models, historic boats and photos |
| Florida Museum of Natural History | | Gainesville | Alachua | North Central | Natural history | Located on the campus of the University of Florida, flora, fauna, fossils and historic peoples of the state of Florida |
| Florida Museum of Photographic Arts | | Tampa | Hillsborough | Central West | Art | Photography, formerly Tampa Gallery of Photographic Arts |
| Florida Pioneer Museum | | Florida City | Miami-Dade | Southeast | Local history | |
| Florida Postal Museum | | Orange City | Volusia | Central East | Philatelic | History of postal items, postal history, and postal service |
| Florida Sports Hall of Fame | | Auburndale | Polk | Central | Sports | Memorabilia of famous Florida athletes |
| Florida Railroad Museum | | Parrish | Manatee | Southwest | Railroad | Heritage railroad along 6 miles of track |
| Florida State Capitol | | Tallahassee | Leon | North Central | History | Florida's legislative history |
| Florida State University Museum of Fine Arts | | Tallahassee | Leon | North Central | Art | website, part of Florida State University |
| Foosaner Art Museum | | Melbourne | Brevard | Central East | Art | Collection includes Modern and Contemporary art, American industrial design, Asian art, and women artists; formerly the Brevard Art Museum |
| Forest Capital State Museum | | Perry | Taylor | North Central | Industrial | Forest industry museum and 1864 Cracker Homestead historic house museum |
| Fort Barrancas | | Pensacola | Escambia | Northwest | Military | Restored mid 19th-century fort, also a visitor center for the Gulf Islands National Seashore |
| Fort Caroline National Memorial | | Jacksonville | Duval | Northeast | History | Operated by U.S. National Park Service |
| Fort Clinch State Park | | Fernandina Beach | Nassau | Northeast | Military | Civil War living history demonstrations on the 1st weekend of the month |
| Fort Christmas Historical Park | | Christmas | Orange | Central | Multiple | Includes restored fort and historic houses, exhibits on military, Seminole and pioneer life |
| Fort De Soto Park | | Tierra Verde | Pinellas | Central West | Military | 1,136-acre park made up of five interconnected islands, Quartermaster Storehouse Museum with displays about the late 19th-century fort |
| Fort East Martello Museum & Gardens | | Key West | Monroe | Florida Keys | Multiple | Local history, art, military memorabilia |
| Fort Foster Historic Site | | Zephyrhills | Pasco | Central West | Military | Restored early 19th century Seminole War fort |
| Fort Lauderdale Antique Car Museum | | Fort Lauderdale | Broward | Southeast | Automotive | Features 22 Packards and automobile memorabilia |
| Fort Lauderdale History Center | | Fort Lauderdale | Broward | Southeast | History | Includes main museum, a historic house museum and a schoolhouse |
| Fort Matanzas National Monument | | St. Augustine | St. Johns | Northeast | Military | 1740 Spanish fort |
| Fort Meade Historical Museum | | Fort Meade | Polk | Central | Local history | Located in the Old Fort Meade School House |
| Fort Mose Historic State Park | | St. Augustine | St. Johns | Northeast | History | Park and museum at site of the first legally sanctioned free African settlement in what is now the United States |
| Fort Walton Beach Heritage Park & Cultural Center | | Fort Walton Beach | Okaloosa | Northwest | Multiple | Includes the Indian Temple Mound Museum with exhibits depicting 12,000 years of Native American occupation and the Fort Walton Temple Mound, the Camp Walton Schoolhouse Museum with early education displays, the Garnier Post Office Museum with postal history items from 1918 to 1956, and the Civil War Exhibits building |
| Fort Zachary Taylor Historic State Park | | Key West | Monroe | Florida Keys | Military | Civil War fort |
| Foster Tanner Fine Arts Gallery | | Tallahassee | Leon | North Central | Art | Gallery at Florida A&M University, focus is art from the African diaspora |
| Fountain of Youth Archaeological Park | | St. Augustine | St. Johns | Northeast | Local history | Site of Ponce de León's Fountain of Youth, includes native and colonial artifacts and historic structures |
| Four Arts Gardens | | Palm Beach | Palm Beach | Southeast | Art | Also known as Philip Hulitar Sculpture Gardens, outdoor sculpture gardens |
| FPL Energy Encounter | | Hutchinson Island | Martin | Central East | Science | website, operated by Florida Power & Light, exhibits about electricity and energy generation, adjacent to the St. Lucie Nuclear Power Plant |
| Frances Wolfson Gallery | | Miami | Miami-Dade | Southeast | Art | At Wolfson Campus, Miami Dade College |
| Fred Dana Marsh Museum | | Ormond Beach | Volusia | Central East | Multiple | Located in Tomoka State Park, art, natural and cultural history |
| Frost Art Museum | | Miami | Miami-Dade | Southeast | Art | At Florida International University, includes American modern sculptures, paintings, and photographs, contemporary Caribbean and Latin American artists |
| Gadsden Arts Center | | Quincy | Gadsden | North Central | Art | Renovated hardware store (1912), with rotating regional & national art exhibitions and a permanent collection of vernacular art. |
| Gamble Plantation Historic State Park | | Ellenton | Manatee | Southwest | Historic house | Antebellum plantation mansion |
| Genova Museum | | Boynton Beach | Palm Beach | Southeast | Multiple | Star Wars, Elvis and Titanic memorabilia |
| Gillespie Museum | | DeLand | Volusia | Central East | Geology | Earth sciences museum at Stetson University |
| Glazer Children's Museum | | Tampa | Hillsborough | Central West | Children's | |
| Gold Coast Railroad Museum | | Miami | Miami-Dade | Southeast | Railroad | Historic railroad cars, equipment and model train building |
| Golisano Children's Museum of Naples | | Naples | Collier | Southwest | Children's | |
| Goodwood Museum and Gardens | | Tallahassee | Leon | North Central | Historic house | World War I period house and gardens |
| Government House Museum | | St. Augustine | St. Johns | Northeast | History | City history back to its founding |
| Grant Historical House | | Grant | Brevard | Central East | Historic House | Local history |
| Great Explorations Children's Museum | | St. Petersburg | Pinellas | Central West | Children's | |
| Groveland Historical Museum | | Groveland | Lake | Central | Local history | Operated by the Groveland Historical Society |
| Gulf Beaches Historical Museum | | St. Pete Beach | Pinellas | Central West | Local history | History of Pinellas County's barrier islands, located in the Pass-a-Grille Historic District |
| Gumbo Limbo Environmental Complex | | Boca Raton | Palm Beach | Southeast | Nature center | |
| Haitian Heritage Museum | | Miami | Miami-Dade | Southeast | Ethnic-Haitian | Art, culture and heritage of Haiti |
| Halifax Historical Museum | | Daytona Beach | Volusia | Central East | Local history | |
| Hannibal Square Heritage Center | | Winter Park | Orange | Central | Ethnic-African American | Operated by the Crealdé School of Art, history, heritage and art of West Winter Park's African American community |
| Harbor Branch Ocean Discovery Center | | Fort Pierce | St. Lucie | Central East | Natural history | Includes exhibits, marine creatures and displays exploring the marine environment and depicting the research efforts of the Harbor Branch Oceanographic Institution |
| Hamilton County Historical Museum | | Jasper | Hamilton | North Central | Prison | Former county jail |
| Hardee Center for the Arts | | Madison | Madison | North Central | Art | website, part of North Florida Community College |
| Harry P. Leu Gardens | | Orlando | Orange | Central | Historic house | Botanical gardens, includes the Leu House Museum, a turn-of-the-20th-century period house |
| Hawthorne Historical Museum & Cultural Center | | Hawthorne | Alachua | Central | Local history | |
| Henry B. Plant Museum | | Tampa | Hillsborough | Central West | Historic house | Turn-of-the-20th-century Victorian hotel with period rooms |
| Heritage Museum of Northwest Florida | | Valparaiso | Okaloosa | Northwest | Local history | History and culture of Okaloosa and Walton Counties |
| Heritage Village | | Largo | Pinellas | Central West | Living | website, 21-acre (85,000 m$^2$) site includes a school, church, sponge warehouse, railroad depot and store and a variety of historic houses, and displays about agriculture, coastal living, community life and tourism; adjacent to The Florida Botanical Gardens |
| Hernando Heritage Museum | | Brooksville | Hernando | Central West | Local history | |
| Highlands Museum of the Arts | | Sebring | Highlands | Central | Art | Emerging art |
| Hillsborough River State Park | | Thonotosassa | Hillsborough | Central West | Military | Includes replica of Fort Foster, an 1837 fort from the Second Seminole War |
| Historic Haile Homestead at Kanapaha Plantation | | Gainesville | Alachua | North Central | Historic house | Mid 19th-century antebellum cotton plantation homestead |
| Historic Homestead Townhall Museum | | Homestead | Miami-Dade | Southeast | Local history | |
| Historic Pensacola Village | | Pensacola | Escambia | Northwest | Multiple | Includes Pensacola Museum of History, Museum of Commerce, Museum of Industry, Lavalle House, Dorr House, Old Christ Church and Lear-Rocheblave House |
| Historic Rossetter House Museum | | Melbourne | Brevard | Central East | Historic house | Victorian period house |
| Historic Smallwood Store | | Chokoloskee | Collier | Southwest | History | Historic general store with original goods and fixtures |
| Historic Spanish Point | | Osprey | Sarasota | Southwest | Multiple | 30-acre (120,000 m$^2$) site with prehistoric middens, a pioneer historic house museum, citrus packing house, trails and gardens |
| HistoryMiami | | Miami | Miami-Dade | Southeast | History | History of southeastern Florida, including Greater Miami, the Florida Keys and the Everglades |
| History of Diving Museum | | Islamorada | Monroe | Florida Keys | Maritime | website, historical diving apparatus including helmets, air pumps, armored suits, accessory gear and sunken treasure |
| Holocaust Memorial on Miami Beach | | Miami Beach | Miami-Dade | Southeast | Art | Outdoor sculpture garden in memory of the Holocaust |
| Holocaust Memorial Resource and Education Center of Florida | | Maitland | Orange | Central | Ethnic-Jewish | website |
| Holocaust Museum of Southwest Florida | | Naples | Collier | Southwest | Ethnic-Jewish | website |
| Homeland Heritage Park | | Bartow | Polk | Central | Open air | Includes a school, church, log cabin and 1880s residence |
| House of Refuge Museum at Gilbert's Bar | | Hutchinson Island | Martin | Central East | Maritime | Former shipwreck life-saving station with turn-of-the-20th-century furnishings |
| Hugh Taylor Birch State Park | | Fort Lauderdale | Broward | Southeast | Multiple | Terramar Visitor Center features exhibits on the natural and local history of the park and area |
| IGFA Fishing Hall of Fame & Museum | | Dania Beach | Broward | Southeast | Sports | Operated by the International Game Fish Association, history of recreational fishing, art, historic artifacts, scientific breakthroughs |
| IMAG History & Science Center | | Fort Myers | Lee | Southwest | History & Science | Hands-on science and history, aquarium exhibits, live small animals |
| Immokalee Pioneer Museum | | Immokalee | Collier | Southwest | Open air | Part of the Collier County Museum System, pioneering and ranching days of Collier County |
| Indian River Citrus Museum | | Vero Beach | Indian River | Central East | Local history | Citrus industry in Indian River County |
| Indian Rocks Beach Historical Museum | | Indian Rocks Beach | Pinellas | Central West | Local history | Operated by the Indian Rocks Historical Society |
| InterAmerican Art Gallery | | Miami | Miami-Dade | Southeast | Art | information, at InterAmerican Campus, Miami Dade College |
| International Swimming Hall of Fame | | Fort Lauderdale | Broward | Southeast | Sports | |
| Jacksonville Fire Museum | | Jacksonville | Duval | Northeast | Firefighting | Also known as the Museum of City Fire of 1901 and the Catherine Street Fire Museum |
| Jacksonville University Life Sciences Museum | | Jacksonville | Duval | Northeast | Natural history | Part of Jacksonville University, includes shells, fossils, bird mounts, bones, animal skins |
| Jewish Museum of Florida | | Miami Beach | Miami-Dade | Southeast | Ethnic-Jewish | |
| John G. Riley House & Museum | | Tallahassee | Leon | North Central | Ethnic-African American | Educational and social contributions of African Americans to Florida's history |
| John Gorrie State Museum | | Apalachicola | Franklin | Northwest | Local history | Also history of inventor John Gorrie |
| Jonathan Dickinson State Park | | Hobe Sound | Martin | Central East | Multiple | Features the Elsa Kimbell Environmental Education and Research Center with exhibits about the area's natural and cultural history |
| Richard and Pat Johnson Palm Beach County History Museum | | West Palm Beach | Palm Beach | Southeast | Local history | Operated by the Historical Society of Palm Beach County |
| Jupiter Inlet Lighthouse & Museum | | Jupiter | Palm Beach | Southeast | Maritime | Lighthouse and museum |
| Karpeles Manuscript Library Museum | | Jacksonville | Duval | Northeast | History | One of nine facilities in the U.S. devoted to storage & presentation of original manuscripts |
| Kendall Art Gallery | | Miami | Miami-Dade | Southeast | Art | website, at Kendall Campus, Miami Dade College |
| Kester Cottages | | Pompano Beach | Broward | Southeast | Historic house | Operated by the Pompano Beach Historical Society, one a local history museum, the other typical of the late 1930s, open by appointment |
| Kennedy Space Center Visitor Complex | | Merritt Island | Brevard | Central East | Aerospace | Includes tours, U.S. Astronaut Hall of Fame, Apollo-Saturn V Center, IMAX films, Astronaut Memorial |
| Kent Gallery at FCCJ | | Jacksonville | Duval | Northeast | Art | web Kent campus |
| Key West Heritage House Museum and Robert Frost Cottage | | Key West | Monroe | Florida Keys | Historic house | |
| Key West Light House and Keeper's Quarters Museum | | Key West | Monroe | Florida Keys | Maritime | Lighthouse and museum |
| Key West Museum of Art & History | | Key West | Monroe | Florida Keys | Multiple | Art and local history |
| Key West Shipwreck Historeum Museum | | Key West | Monroe | Florida Keys | Maritime | Combines actors, films and actual artifacts from the 1985 rediscovery of the wrecked vessel Isaac Allerton |
| Kingsley Plantation | | Jacksonville | Duval | Northeast | Open air | Operated by U.S. National Park Service, remains of an early 19th-century plantation with slave houses |
| Kissimmee Air Museum | | Kissimmee | Osceola | Central | Aviation | Formerly known as the Flying Tigers Warbird Restoration Museum |
| Knott House Museum | | Tallahassee | Leon | North Central | Historic house | Operated by the Museum of Florida History, mid 19th-century house with Victorian furnishings |
| Koreshan State Historic Site | | Estero | Lee | Southwest | Open air | Remaining buildings of 20th century religious settlement |
| LaBelle Heritage Museum | | LaBelle | Hendry | Southwest | Local history | |
| Lady Lake Historical Society Museum | | Lady Lake | Lake | Central | Local history | Exhibits include area citrus industry, a miniature HO gauge railroad, antique furniture, tools, photographs and memorabilia |
| Lake City-Columbia County Historical Museum | | Lake City | Columbia | North Central | Local history | website |
| Lake County Historical Museum | | Tavares | Lake | Central | Local history | |
| Lake County Museum of Art | | Eustis | Lake | Central | Art | |
| Lake Kissimmee State Park - Cow Camp | | Lake Wales | Polk | Central | Living | 1876 period cow camp on weekends from Oct. through May 1 |
| Lake Mary Historical Museum | | Lake Mary | Seminole | Central | Local history | |
| Lake Wales Museum and Cultural Center | | Lake Wales | Polk | Central | Local history | Located in a depot, includes a model railroad layout |
| Lake Worth Historical Museum | | Lake Worth | Palm Beach | Southeast | Local history | Includes historic photos, maps, clothing, household items, area ethnic culture |
| Larimer Arts Center | | Palatka | Putnam | Northeast | Art | Art center with gallery |
| LaVilla Museum | | Jacksonville | Duval | Northeast | Ethnic-African American | |
| Leepa-Rattner Museum of Art | | Tarpon Springs | Pinellas | Central West | Art | Part of St. Petersburg College, includes many works by Abraham Rattner and other 20th century artists |
| Leesburg Heritage Museum | | Leesburg | Lake | Central | Local history | |
| LeMoyne Center for the Visual Arts | | Tallahassee | Leon | North Central | Art | website, includes sculpture garden and exhibit galleries |
| Lewis and Lucretia Taylor House Museum of African-American History and Culture | | Tallahassee | Leon | North Central | Ethnic-African American | Also known as the Taylor House Museum, history of the black community in Tallahassee and civil rights |
| Liberty Bell Memorial Museum | | Melbourne | Brevard | Central East | History | Houses military war exhibits, facsimiles of important documents in U.S. history & a full size replica of the Liberty Bell. |
| Lighthouse ArtCenter | | Jupiter | Palm Beach | Southeast | Art | website, museum, gallery and school of art |
| Lightner Museum | | St. Augustine | St. Johns | Northeast | Multiple | Victorian decorative arts, natural history artifacts, antique steam engines, musical instruments, paintings, sculpture, and furniture |
| Loggerhead Marinelife Center | | Juno Beach | Palm Beach | Southeast | Natural history | Rescue and education center for sea turtles |
| Lowe Art Museum | | Coral Gables | Miami-Dade | Southeast | Art | Part of University of Miami, collection includes Greco-Roman antiquities, Renaissance, Baroque, 17th- and 19th-century European art, 19th-century American art, modern art, international works, glass |
| Lyons Maritime Museum | | St. Augustine | St. Johns | Northeast | Maritime | Diving equipment and history |
| Madame Tussauds Orlando | | Orlando | | | Wax | |
| Maitland Art Center | | Maitland | Orange | Central | Art | Includes exhibit gallery |
| Maitland Historical Museum | | Maitland | Orange | Central | Local history | Operated by the Maitland Historical Society, area pioneers |
| Maitland Telephone Museum | | Maitland | Orange | Central | Technology | Operated by the Maitland Historical Society, development of the Winter Park Telephone Company and the technological growth of Maitland |
| Man in the Sea Museum | | Panama City | Bay | Northwest | Maritime | History of underwater diving |
| Manatee County Agricultural Museum | | Palmetto | Manatee | Southwest | Agriculture | Part of Palmetto Historical Park |
| Manatee Village Historical Park | | Bradenton | Manatee | Southwest | Open air | Includes 1903 Wiggins General Store, boatworks, 1912 Florida pioneer farmhouse, smokehouse and sugar cane mill, barn, church, school, courthouse, Cow Hunter's bunkhouse, steam engine |
| Mandarin Museum | | Jacksonville | Duval | Northeast | Historic house | Located in Walter Jones Historical Park, turn-of-the-20th-century period farmhouse |
| Marco Island Historical Museum | | Marco Island, Florida | Collier | Southwest | Local history | Part of the Collier County Museum System, history of the Calusa Indians and the development of Marco Island |
| Marine Science Center | | Ponce Inlet | Volusia | Central East | Natural history | Aquariums, touch tanks, rehabilitated seabirds and sea turtles |
| Marion County Museum of History | | Ocala | Marion | Central | Local history | |
| Maritime & Yachting Museum | | Stuart | Martin | Central East | Maritime | Includes antique and classic boats, engines, model ships, navigation equipment, paintings and photographs |
| Marjorie Kinnan Rawlings Historic State Park | | Cross Creek | Alachua | North Central | Farm | Working farm of author Marjorie Kinnan Rawlings |
| Mary Brogan Museum of Art and Science | | Tallahassee | Leon | North Central | Multiple | Science and art, with collections in American Folk art, photography, sculpture, paintings and drawings, prints, and Pre-Columbian art |
| Mary McLeod Bethune Home | | Daytona Beach | Volusia | Central East | Historic house | Home of Mary McLeod Bethune, an educator and civil rights leader |
| Matheson Museum | | Gainesville | Alachua | North Central | Multiple | Includes the local history museum, the historic Matheson House, and the Tison Tool Museum, operated by the Alachua County Historic Trust |
| Mattie Kelly Arts Center | | Niceville | Okaloosa | Northwest | Art | Performing art center with exhibit gallery, part of Northwest Florida State College |
| McLarty Treasure Museum | | Vero Beach | Indian River | Central East | Maritime | Exhibits on the 1715 Spanish treasure fleet |
| MDC Museum of Art and Design | | Miami | Miami-Dade | Southeast | Art | website, operated by Miami Dade College, located in the Freedom Tower |
| Mel Fisher Maritime Heritage Museum | | Key West | Monroe | Florida Keys | Maritime | |
| Mel Fisher's Treasure Museum | | Sebastian | Indian River | Central East | Maritime | Shipwreck finds |
| Mennello Museum of American Art | | Orlando | Orange | Central | Art | American art including traditional and contemporary art, features collection of primitive art by Earl Cunningham |
| Merrill House Museum | | Jacksonville | Duval | Northeast | Historic house | Victorian-era house, operated by the Jacksonville Historical Society |
| Miami Children's Museum | | Miami | Miami-Dade | Southeast | Children's |
| Miami-Dade Military Museum | | Miami | Miami-Dade | Southeast | Military |
| Micanopy Historical Society Museum | | Micanopy | Alachua | North Central | Local history | |
| Middleburg Historical Museum | | Middleburg | Clay | Northeast | Local history | |
| Military Heritage & Aviation Museum | | Punta Gorda | Charlotte | Southwest | Military | |
| Military Museum of North Florida | | Green Cove Springs | Clay | Northeast | Military | Includes ship models, military vehicles, uniforms, weapons, equipment, flags |
| Military Sea Services Museum | | Sebring | Highlands | Central | Military | |
| Mission San Luis | | Tallahassee | Leon | North Central | Living | Recreated Colonial Spanish mission village, with Apalachee village, the council house, and the home of the Spanish Deputy Governor |
| Monticello Old Jail Museum | | Monticello | Jefferson | Northwest | Prison | Facebook site, former jail with prison cells, displays of local history |
| Morean Arts Center | | St. Petersburg | Pinellas | Central West | Art | Displays works by local, national and international artists |
| Morikami Museum and Japanese Gardens | | Delray Beach | Palm Beach | Southeast | Ethnic-Japanese | Japanese art and culture |
| Morningside Nature Center | | Gainesville | Alachua | North Central | Farm | Weekend living history programs, working farm and nature programs |
| Morse Museum | | Winter Park | Orange | Central | Art | Also known as the Charles Hosmer Morse Museum of American Art, American decorative arts including works by Tiffany, American art pottery, late-19th and early-20th-century American paintings, graphics, decorative arts and the Tiffany Chapel |
| Mote-Morris House | | Leesburg | Lake | Central | Historic house | Victorian house, open twice a month |
| Mound House | | Fort Myers Beach | Lee | Southwest | Local history | website, tours of an ancient Calusa Indian Mound, exhibits of archaeology, history and natural history, 1920s house under restoration |
| Mount Dora Center for the Arts | | Mount Dora | Lake | Central | Art | |
| Mount Dora History Museum | | Mount Dora | Lake | Central | Local history | Formerly the Royellou Museum, contains vintage artifacts housed in the Old City Jail |
| Mount Dora Museum of Speed | | Mount Dora | Lake | Central | Automotive | Includes muscle cars, automobile memorabilia, vintage road signs, Americana collection |
| Mulberry Phosphate Museum | | Mulberry | Polk | Central | Multiple | Includes fossils, area memorabilia and exhibits about the phosphate industry |
| Museum at Ragtops Motorcars | | Palm Beach | Palm Beach | Southeast | Automotive | website, vintage automobiles and memorabilia |
| Museum of Art Fort Lauderdale | | Fort Lauderdale | Broward | Southeast | Art | Contemporary, southeastern Florida and Caribbean art |
| Museum of Arts and Sciences | | Daytona Beach | Volusia | Central East | Multiple | Also called MOAS, includes art, science, Americana, historic house museums, a children's museum, Cuban Museum gallery |
| Museum of Contemporary Art | | North Miami | Miami-Dade | Southeast | Art | |
| Museum of Contemporary Art Jacksonville | | Jacksonville | Duval | Northeast | Art | Also known as MOCA Jacksonville |
| Museum of Dinosaurs and Ancient Cultures | | Cocoa Beach | Brevard | Central | Natural history | Dinosaur dioramas, fossils, artifacts and dioramas of ancient Egypt, China and Mesoamerica |
| Museum of Discovery and Science | | Fort Lauderdale | Broward | Southeast | Science | |
| Museum of Lifestyle & Fashion History | | | Palm Beach | Southeast | Fashion | Currently seeking permanent location, exhibits of fashion, Barbie dolls, design |
| Museum of Fine Arts | | St. Petersburg | Pinellas | Central West | Art | Collections include European and American art, photography, Greek and Roman antiquities, pre-Columbian and Asian art |
| Museum of Florida Art | | DeLand | Volusia | Central East | Art | Formerly the DeLand Museum of Art, focus is Florida art |
| Museum of Florida Art and Culture | | Avon Park | Highlands | Central | Art | Part of South Florida Community College, includes art, archaeology, and Florida history exhibits |
| Museum of Florida History | | Tallahassee | Leon | North Central | History | The official history Museum of the State of Florida. Website. |
| Museum of Geneva History | | Geneva | Seminole | Central East | Local history | Operated by the Geneva Historical & Genealogical Society |
| Museum of Local History | | Milton | Santa Rosa | Northwest | Local history | |
| Museum of Polo & Hall of Fame | | Lake Worth | Palm Beach | Southeast | Sports | History of polo |
| Museum of Science & History | | Jacksonville | Duval | Northeast | Multiple | Science, natural history, history of Northeast Florida, planetarium |
| Museum of Science and Industry | | Tampa | Hillsborough | Central West | Science | Exhibits on science, weather, health |
| Museum of Seminole County History | | Sanford | Seminole | Central | Local history | |
| Museum of Southern History | | Jacksonville | Duval | Northeast | History | Also called G. Howard Bryan Museum of Southern History, lifestyle and culture of the antebellum American South |
| Museum of the Americas | | Doral | Miami-Dade | Southeast | Art | website, contemporary art |
| Museum of the Apopkans | | Apopka | Orange | Central | Local history | |
| Museum of the City of Lake Worth | | Lake Worth | Palm Beach | Southeast | Local history | |
| Museum of the Everglades | | Everglades City | Collier | Southwest | Local history | Part of the Collier County Museum System, dedicated to the building of the Tamiami Trail and the founding of Everglades City |
| Museum of the Glades | | Belle Glade | Palm Beach | Southeast | Local history | Includes artifacts of the Belle Glade culture, pioneer and area history |
| Museum of the Islands | | Pine Island Center | Lee | Southwest | Local history | Exhibits include shells, dolls, household items, fishing artifacts |
| My Jewish Discovery Place Children's Museum | | Plantation | Broward | Southeast | Ethnic-Jewish | website, hands-on, interactive museum of Jewish culture, history and values |
| Naples Depot Museum | | Naples | Collier | Southwest | Transportation | Part of the Collier County Museum System, exhibits describe how technology and transportation were used to found the City of Naples |
| The Baker Museum | | Naples | Collier | Southwest | Art | Includes American modernism, 20th-century Mexican art, sculpture and 3-dimensional art |
| Nathan H. Wilson Center for the Arts | | Jacksonville | Duval | Northeast | Art | website, part of Florida State College at Jacksonville, South campus, includes an art gallery |
| National Armed Services & Law Enforcement Memorial Museum | | Dunedin | Pinellas | Central West | Law enforcement | website |
| National Museum of Naval Aviation | | Pensacola | Escambia | Northwest | Aviation | History of naval aviation |
| National Navy UDT-SEAL Museum | | Fort Pierce | St. Lucie | Central East | Military | History of the Navy Underwater Demolition Teams (UDT) and Sea, Air, Land (SEAL) Teams |
| New Smyrna Museum of History | | New Smyrna Beach | Volusia | Central East | Local history | |
| Norman Studios Silent Film Museum | | Jacksonville | Duval | Northeast | Cinema | Historic film studio that made films starring African-American actors in non-stereotypic roles |
| North Brevard Historical Museum | | Titusville | Brevard | Central East | Local history | |
| North Gallery | | Miami | Miami-Dade | Southeast | Art | At North Campus, Miami Dade College |
| North Pinellas Historical Museum | | Palm Harbor | Pinellas | Central West | Local history | History of the Palm Harbor, Crystal Beach, and the Ozona and Curlew communities |
| Norton Museum of Art | | West Palm Beach | Palm Beach | Southeast | Art | Collections include European, American, and Chinese art, contemporary art and photography |
| Old Courthouse Heritage Museum | | Inverness | Citrus | Central West | Local history | |
| Old Davie School Museum | | Davie | Broward | Southeast | Local history | |
| Old Deerfield School | | Deerfield Beach | Broward | Southeast | Local history | |
| Old Dillard Museum | | Fort Lauderdale | Broward | Southeast | Ethnic-African American | |
| Old Florida Museum | | St. Augustine | St. Johns | Northeast | History | Hands-on programs with daily living activities from pre-European times to 20th century |
| Old Jail Museum | | St. Augustine | St. Johns | Northeast | Local history | Also known as Florida Heritage Museum |
| Old Town Hall History Center | | Melbourne Beach | Brevard | Central East | Local history | |
| Oldest House Museum | | Key West | Monroe | Florida Keys | Historic house | website, operated by the Old Island Restoration Foundation, 1829 house with original furnishings, ship models, maritime artifacts, items recovered from Keys waters |
| Oldest House Museum Complex | | St. Augustine | St. Johns | Northeast | Local history | Operated by the St. Augustine Historical Society, includes the Gonzalez-Alvarez House, the Manucy Museum of local history, and the Edwards Gallery with changing exhibits |
| Oldest Store Museum | | St. Augustine | St. Johns | Northeast | History | Historic general store with early 20th-century items and re-enactors |
| Oldest Wooden Schoolhouse | | St. Augustine | St. Johns | Northeast | Education | 1807 period schoolhouse |
| Olustee Battlefield Historic State Park | | Olustee | Baker | Northeast | Local history | American Civil War battlefield and visitor center museum |
| Orange County Regional History Center | | Orlando | Orange | Central | Local history | |
| Orlando Auto Museum (Dezerland Park Orlando) | | Orlando | Orange | Central | Automotive | Museum with over 2,000 vehicles |
| Orlando Fire Museum | | Orlando | Orange | Central | Firefighting | website, located in a historic firehouse |
| Orlando Museum of Art | | Orlando | Orange | Central | Art | |
| Orlando Science Center | | Orlando | Orange | Central | Science | |
| Orman House | | Apalachicola | Franklin | Northwest | Historic house | 1838 antebellum home |
| Ormond Memorial Art Museum and Gardens | | Ormond Beach | Volusia | Central East | Art | Includes a botanical garden, features works by Malcolm Fraser |
| Osceola Center for the Arts | | Kissimmee | Osceola | Central | Art | website |
| Osceola County Welcome Center and History Museum | | Kissimmee | Osceola | Central | Open air | Includes the Osceola County Historical Society Museum with local history exhibits, and the Pioneer Village with the 1889 Ross Lanier House (1889), one-room schoolhouse, citrus packing house (1882), cow camp |
| Palm Beach Maritime Museum | | Riviera Beach | Palm Beach | Southeast | Maritime | website, includes tours of the historic Coast Guard facility and a bomb shelter on Peanut Island |
| Palm Beach Photographic Centre | | West Palm Beach | Palm Beach | Southeast | Photography | Photography exhibits, education |
| Palm Cottage | | Naples | Collier | Southwest | Historic house | Early 20th century period house and gardens |
| Palmetto Historical Park | | Palmetto | Manatee | Southwest | Open air | Includes local history museum, agriculture museum, cottage museum with antiques and dolls, church, school, post office |
| Panama City Center for the Arts | | Panama City | Bay | Northwest | Art | Hosts shows of fine art, sculpture, jewelry and ceramics |
| Panhandle Pioneer Settlement | | Blountstown | Calhoun | Northwest | Living | website, recreates an early agricultural community with a pioneer settlement and a farmstead |
| Pasco Arts Center | | Holiday | Pasco | Central West | Art | Art exhibits, classes and workshops |
| Paynes Creek Historic State Park | | Bowling Green | Hardee | Central | History | Frontier trading post site, exhibits about Seminoles, the trading post, frontier fort and pioneers |
| Pena-Peck House | | St. Augustine | St. Johns | Northeast | Historic house | website, operated by the St. Augustine Woman's Exchange, 18th-century Spanish Colonial house |
| Pensacola Lighthouse & Museum | | Pensacola | Escambia | Northwest | Maritime | |
| Pensacola Museum of Art | | Pensacola | Escambia | Northwest | Art | Includes 20th century and contemporary art, glass and African tribal art |
| Pérez Art Museum Miami | | Miami | Miami-Dade | Southeast | Art | Contemporary art, formerly the Miami Art Museum |
| Phillip and Patricia Frost Museum of Science | | Miami | Miami-Dade | Southeast | Science | Science and natural history, formerly the Miami Science Museum |
| Pioneer Florida Museum and Village | | Dade City | Pasco | Central West | Open air | Includes Overstreet House, a one-room schoolhouse, church, depot, a train engine, and museum with tools, household items and antiques |
| Pioneer Heritage Museums | | Plant City | Hillsborough | Central West | Local history | website, East Hillsborough Historical Society Facebook site |
| Pioneer Settlement for the Creative Arts | | Barberville | Volusia | Central East | Open air | Late 19th and early 20th century buildings and trades |
| Plantation Historical Museum | | Plantation | Broward | Southeast | Local history | website, adjacent to the town's botanical park and arboretum |
| Plumb House Museum | | Clearwater | Pinellas | Central West | Local history | |
| Polk State College Fine Arts Gallery | | Winter Haven | Polk | Central | Art | Part of Polk State College |
| Polk County Historical Museum | | Bartow | Polk | Central | Local history | Located in the Old Polk County Courthouse |
| Polk County Nature Discovery Center | | Lakeland | Polk | Central | Natural history | Polk County's water, wilderness and wildlife, hands-on exhibits, live aquatic plans and animals in aquariums |
| Polk Museum of Art | | Lakeland | Polk | Central | Art | Includes, African, Asian, Pre-Columbian and contemporary art, European and American decorative arts |
| Ponce de Leon Inlet Light | | Ponce Inlet | Volusia | Central East | Maritime | Lighthouse and museum |
| Port Boca Grande Lighthouse & Museum | | Boca Grande | Lee | Southwest | Local & Natural History | Active lighthouse & museum, managed & operated by Barrier Island Parks Society (BIPS) |
| Potter's Wax Museum | | St. Augustine | St. Johns | Northeast | Miscellaneous | Over 160 wax sculptures covering a wide range of real & fictitious figures. |
| Presidents Hall of Fame | | Clermont | Lake | Central | Wax | website, next to the Florida Citrus Tower, wax figures of U.S. presidents, authentic miniature replicas of rooms in the White House, and other representations of Americana |
| Punta Gorda Historical Museum | | Punta Gorda | Charlotte | Southwest | Local history | Operated by the Punta Gorda Historical Society in the A. C. Freeman House |
| Putnam Historic Museum | | Palatka | Putnam | Northeast | Local history | website, information, operated by the Putnam County Historical Society |
| Railroad Museum of South Florida | | Fort Myers | Lee | Southwest | Railroad | website, located in Lakes Regional Park |
| Randall Knife Museum | | Orlando | Orange | Central | Knives | Displays of custom made knives. |
| Raney House Museum | | Apalachicola | Franklin | Northwest | Historic house | 19th century period house |
| Ransom Arts Center | | St. Petersburg | Pinellas | Central West | Art | website, Cobb and Elliott Galleries, part of Eckerd College |
| Ringling College of Art and Design Galleries | | Sarasota | Sarasota | Southwest | Art | website, part of Ringling College of Art and Design, includes the Selby, Crossley, Goldstein and Hammond Commons galleries |
| Ringling Museum of Art | | Sarasota | Sarasota | Southwest | Multiple | Formally called The John and Mable Ringling Museum of Art, includes European paintings, Cypriot antiquities and Asian, American, and contemporary art, decorative arts, Ringling's mansion, and a circus museum |
| Ripley's Believe It or Not! Museum | | St. Augustine | St. Johns | Northeast | Miscellaneous | Home of Ripley's personal collections located in Castle Warden, a Moorish Revival style mansion built in 1887. |
| Rookery Bay National Estuarine Research Reserve | | Naples | Collier | Southwest | Natural history | Natural and cultural history of the reserve |
| Retro Gaming Museum, LLC | Retro Gaming Museum | Cocoa | Brevard | Central East | Retro Gaming | 72+ Exabits Photos, Art Interactive Exabits |
| Rosemary Duffy Larson Gallery, Broward College | | Davie | Broward | Southeast | Art | website, part of Broward College (A. Hugh Adams Campus) |
| Rubell Museum | | Miami | Miami-Dade | Southeast | Art | website, contemporary art |
| Ruth Funk Center for Textile Arts | | Melbourne | Brevard | Central East | Art | Part of Florida Institute of Technology; textiles, clothing and accessories |
| Safety Harbor Museum and Cultural Center | | Safety Harbor | Pinellas | Central West | Local history | website, city and regional history |
| Safford House Museum | | Tarpon Springs | Pinellas | Central West | Historic house | Late 19th century Victorian house |
| Salvador Dalí Museum | | St. Petersburg | Pinellas | Central West | Art | Art by Salvador Dalí, including oil paintings, watercolors and drawings, graphics, photographs, sculptures and objets d'art |
| Samuel Baker House | | Holiday | Pasco | Central West | Historic house | Late 19th-century house, operated by the West Pasco Historical Society |
| Samuel P. Harn Museum of Art | | Gainesville | Alachua | North Central | Art | Part of the University of Florida, includes Asian, African, modern and contemporary art, photography |
| San Carlos Institute | | Key West | Monroe | Florida Keys | Ethnic-Cuban | Cuba's history and the history of the Cuban-American community in Florida |
| San Marcos de Apalache Historic State Park | | St. Marks | Wakulla | North Central | Military | History of the 17th-century fort and excavated items |
| Sandoway Discovery Center | | Delray Beach | Palm Beach | Southeast | Natural history | Exhibits include a large shell collection, local natural and cultural history, tortoises and turtles, live animals and marine creatures |
| Sanford Museum | | Sanford | Seminole | Central | Local history | website |
| Sarasota Classic Car Museum | | Sarasota | Sarasota | Southwest | Automotive | |
| Scarfone/Hartley Gallery | | Tampa | Hillsborough | Central West | Art | website at University of Tampa |
| Schoolhouse Children's Museum | | Boynton Beach | Palm Beach | Southeast | Children's | |
| Science Center of Pinellas County | | St. Petersburg | Pinellas | Central West | Science | Science, natural history, African-American scientists and inventors, live animals, replica 16th Century Native American village, observatory, planetarium |
| Science and Discovery Center of Northwest Florida | | Panama City | Bay | Northwest | Children's | Formerly the Junior Museum of Bay County, hands-on science and natural history exhibits, children's discovery areas, trail |
| Sebastian Fishing Museum | | Melbourne Beach | Brevard | Central East | Maritime | Part of Sebastian Inlet State Park, area fishing industry |
| Seminole County Public Schools Student Museum | | Sanford | Seminole | Central | Local history | |
| Silver River Museum and Environmental Center | | Ocala | Marion | Central | Multiple | Area natural and cultural history, late 19th century pioneer village |
| Skeletons: Museum of Osteology | | Orlando | Orange | Central | Natural history | website, over 450 animal skeletons creatively posed and in dioramas |
| Society of the Four Arts Galleries | | Palm Beach | Palm Beach | Southeast | Art | Includes the Esther B. O'Keeffe Art Gallery and the Mary Alice Fortin Children's Art Gallery |
| South Florida Museum | | Bradenton | Manatee | Southwest | Natural history | Natural and cultural history of Florida's gulf coast, includes the Bishop Planetarium and Parker Manatee Aquarium |
| South Florida Railway Museum | | Deerfield Beach | Broward | Southeast | Railroad | Includes model train layouts, railroad artifacts |
| South Florida Science Center and Aquarium | | West Palm Beach | Palm Beach | Southeast | Science | Includes a planetarium and aquarium |
| South Gallery at Broward Community College | | Pembroke Pines | Broward | Southeast | Art | website, at Broward Community College South Campus |
| Southeast Museum of Photography | | Daytona Beach | Volusia | Central East | Art | Photography |
| Southeastern Regional Black Archives Research Center and Museum | | Tallahassee | Leon | North Central | Ethnic-African American | African American history in Florida, also a branch located in the Union Bank |
| Southwest Florida Museum of History | | Fort Myers | Lee | Southwest | Local history | Exhibits include Paleo Indians, the Calusa, the Seminoles, Spanish explorers and early settlers, cattlemen, military and agriculture, boating and fishing industries; period antiques and decorative arts; a pioneer "cracker" house, 1926 La France fire pumper, and 1929 private Pullman rail car |
| Spady Cultural Heritage Museum | | Delray Beach | Palm Beach | Southeast | Ethnic-African American | African American history, culture and heritage in Florida |
| Spanish Military Hospital Museum | | St. Augustine | St. Johns | Northeast | Medical | Military hospital life in 1791 |
| Spongeorama | | Tarpon Springs | Pinellas | Central West | Maritime | Sponge diving & harvesting in Gulf of Mexico |
| St. Augustine Lighthouse & Maritime Museum | | St. Augustine | St. Johns | Northeast | Maritime | On an historic light station, the non-profit museum includes an 1876 lighthouse tower, keepers' house, WWII era restorations, and a maritime museum with shipwreck artifacts discovered via an in-house scientific diving program. Shares the stories of the maritime history of the oldest continually occupied port city in the fifty states. The aid-to-navigation is privately owned and operated by the Museum which is accredited by the American Alliance of Museums. |
| St. Augustine Pirate & Treasure Museum | | St. Augustine | St. Johns | Northeast | Maritime | Pirate artifacts, formerly the Pirate Soul Museum in Key West |
| St. Cloud Heritage Museum | | St. Cloud | Osceola | Central | Local history | Photographs, documents and artifacts of local history and culture, operated by the Woman's Club of St. Cloud |
| St. Lucie County Regional History Center | | Fort Pierce | St. Lucie | Central East | Local history | |
| St. Petersburg Museum of History | | St. Petersburg | Pinellas | Central West | Local history | |
| St. Photios Greek Orthodox National Shrine | | St. Augustine | St. Johns | Northeast | Religious | Site of first Greek settlement in America |
| Stephen Foster Folk Culture Center State Park | | White Springs | Hamilton | North Central | Biographical | Museum about composer Stephen Foster and carillon tower that plays his songs |
| Stranahan House | | Fort Lauderdale | Broward | Southeast | Historic house | 1913 period house |
| Stuart Heritage Museum | | Stuart | Martin | Central East | Local history | Housed in a former feed supply store |
| Sulphur Springs Museum and Heritage Center | | Tampa | Hillsborough | Central West | Local history | |
| Suwannee County Historical Museum | | Live Oak | Suwanee | North Central | Local history | Housed in a former railroad depot |
| Pensacola Museum of History | | Pensacola | Escambia | Northwest | Multiple | Part of Historic Pensacola Village, includes historic artifacts, Americana, West Florida history, period store displays, antique transportation, antiques |
| Tallahassee Automobile Museum | | Tallahassee | Leon | North Central | Automotive | Historic cars, toy cars, pedal cars, antique boating items, golf memorabilia, motors, cash registers, clocks, bicycles, sports memorabilia, motorcycles, can openers, spark plugs, artifacts |
| Tallahassee Museum | | Tallahassee | Leon | North Central | Multiple | Includes an open air section with an 1890s school, a 1937 church, and three buildings of the mid-19th century Bellevue Plantation, a living history farm, live wildlife, a children's discovery center, a display gallery for art and cultural exhibits, and trails |
| Tampa Baseball Museum | | Tampa | Hillsborough | Central West | Baseball | Former home of Al López, honors the lives of 89 professional players |
| Tampa Bay Automobile Museum | | Pinellas Park | Pinellas | Central West | Automotive | Historic automobiles from the 20th century |
| Tampa Bay History Center | | Tampa | Hillsborough | Central West | Local history | Covers 12,000 years of Florida history with a special focus on Tampa Bay and the Gulf Coast |
| Tampa Firefighters Museum | | Tampa | Hillsborough | Central West | Firefighting | website |
| Tampa Museum of Art | | Tampa | Hillsborough | Central West | Art | Collections include 20th-century fine art, Greek, Roman, and Etruscan antiquities |
| Tarpon Springs Cultural Center | | Tarpon Springs | Pinellas | Central West | Art | Changing art and sculpture exhibits |
| Tarpon Springs Heritage Museum | | Tarpon Springs | Pinellas | Central West | Local history | website, area's sponge diving, Native Americans, Greek immigrants and local area history |
| Ted Williams Museum and Hitters Hall of Fame | | St. Petersburg | Pinellas | Central West | Sports | Located in Tropicana Field, baseball and Ted Williams history and memorabilia |
| Thomas Center | | Gainesville | Alachua | North Central | Multiple | Changing art exhibits, local history and 1920s period hotel rooms |
| Titanic The Experience | | Orlando | Orange | Central | Maritime | website, includes re-creations of actual rooms from the ship RMS Titanic, artifacts, and movie memorabilia |
| Torreya State Park - Gregory House | | Bristol | Liberty | Northwest | Historic house | 1840s plantation house |
| Treasures of Madison County | | Madison | Madison | North Central | Local history | Area history and culture |
| Tree Hill Nature Center | | Jacksonville | Duval | Northeast | Natural history | Features live animals, wildlife sanctuary |
| Truman Little White House | | Key West | Monroe | Florida Keys | Historic house | Winter White House for President Harry S. Truman |
| Umatilla Historical Museum | | Umatilla | Lake | Central | Local history | Located in the Paul W. Bryan Historic Schoolhouse |
| UNF Gallery of Art | | Jacksonville | Duval | Northeast | Art | website, part of University of North Florida |
| University Galleries, University of Florida | | Gainesville | Alachua | North Central | Art | website, part of the University of Florida |
| University of Central Florida Art Gallery | | Orlando | Orange | Central | Art | website, part of the University of Central Florida |
| University of South Florida Contemporary Art Museum | | Tampa | Hillsborough | Central West | Art | Part of the University of South Florida, contemporary art including graphics and sculpture |
| University of West Florida Art Gallery | | Pensacola | Escambia | Northwest | Art | website, part of University of West Florida |
| USCGC INGHAM Maritime Museum | | Key West | Monroe | Florida Keys | Maritime | United States Coast Guard Cutter and memorial to guardians killed in action in World War II and Vietnam |
| USS Mohawk CGC Memorial Museum | | Key West | Monroe | Florida Keys | Maritime | World War II US Coast Guard cutter museum ship |
| Valiant Air Command Warbird Museum | | Titusville | Brevard | Central East | Aviation | Fixed and rotary wing military aircraft from World War I to the present |
| Venice Art Center | | Venice | Sarasota | Southwest | Art | web |
| Vernon Historical Society Museum | | Vernon | Washington | Northwest | Local history | Photographs, people, tools |
| Vero Beach Museum of Art | | Vero Beach | Indian River | Central East | Art | Art from the early 20th century to the present in a broad range of media |
| Villa Zorayda | | St. Augustine | St. Johns | Northeast | Historic house | Scaled-down version of the 12th-century Moorish Alhambra Palace in Granada, Spain |
| Vizcaya Museum and Gardens | | Miami | Miami-Dade | Southeast | Historic house | 70 room estate with 15th through early 19th century furnishings and European decorative art, also botanical gardens |
| Von Liebig Art Center | | Naples | Collier | Southwest | Art | website, operated by the Naples Art Association, contemporary art |
| Walton County Heritage Museum | | DeFuniak Springs | Walton | Northwest | Local history | |
| Washington County Historical Museum | | Chipley | Washington | Northwest | Local history | Operated by the Washington County Historical Society in a former train depot |
| Waterhouse Residence and Carpentry Shop Museums | | Maitland | Orange | Central | Historic house | Victoria era family home and adjacent carpentry shop, operated by the Maitland Historic Society |
| Weedon Island Preserve Cultural and Natural History Center | | St. Petersburg | Pinellas | Central West | Multiple | Native Americans, natural history, art and local history |
| Wells'Built Museum | | Orlando | Orange | Central | Ethnic-African American | |
| West Florida Railroad Museum | | Milton | Santa Rosa | Northwest | Railroad | Railroad cars and memorabilia from the L & N, Frisco and other railroads |
| West Pasco Historical Society Museum and Library | | New Port Richey | Pasco | Central West | Local history | Exhibits include Native American arrowhead and artifacts, clothing, household items, antiques and decorative items, tools, and historic photographs |
| Wings of Dreams Aviation Museum | | Starke | Clay | Northeast | Aviation | Located at Keystone Heights Airport |
| Wings Over Miami Museum | | Miami | Miami-Dade | Southeast | Aviation | Located at Kendall-Tamiami Executive Airport, historic flying aircraft |
| Winter Garden Heritage Museum | | Winter Garden | Orange | Central | Local history | Exhibits include local citrus labels, Native American artifacts, railroad memorabilia |
| Winter Park History Museum | | Winter Park | Orange | Central | Local history | Operated by the Winter Park Historical Association |
| Wizard of Oz Museum | | Cape Canaveral | Brevard | Central East | Culture | Features a large collection of over 3,000 Wizard of Oz artifacts and memorabilia, including the first known published copy of the original book. |
| Wolfsonian-FIU | | Miami | Miami-Dade | Southeast | Art | Part of Florida International University, late-19th and early-20th century design |
| WonderWorks | | Orlando | Orange | Central | Science | Interactive hands-on science exhibits |
| WonderWorks | | Panama City | Bay | Northwest | Science | Interactive hands-on science exhibits |
| World Erotic Art Museum | | Miami | Miami-Dade | Southeast | Art | Includes art, sculpture, tapestries and artifacts of erotic art |
| World Golf Hall of Fame | | St. Augustine | St. Johns | Northeast | Sports | |
| Ximenez-Fatio House | | St. Augustine | St. Johns | Northeast | Historic house | 19th century territorial and early statehood period life |
| Ybor City Museum State Park | | Tampa | Hillsborough | Central West | Local history | History of the local cigar industry and the immigrant communities there from the 1880s through the 1930s |
| Yesteryear Village | | West Palm Beach | Palm Beach | Southeast | Open air | Heritage park at the South Florida Fair, includes a school, farm, blacksmith shop, general store, several historic houses, and the Sally Bennett Big Band Hall of Fame Museum |
| Young at Art Museum | | Davie | Broward | Southeast | Children's | Early childhood development through exhibits and activities in art, culture, the environment |
| Zephyrhills Depot Museum | | Zephyrhills | Pasco | Central West | Local history | Includes local history, railroad history and memorabilia |
| Zephyrhills Museum of Military History | | Zephyrhills | Pasco | Central West | Military | website, World War II memorabilia, located in a World War II barracks building for the former Zephyrhills Army Airfield |
| Zora Neale Hurston National Museum of Fine Arts | | Eatonville | Orange | Central | Art | Also known as The Hurston, work of artists of African descent, including Zora Neale Hurston |

## Defunct museums

* Armed Forces History Museum, Largo, closed on January 29, 2017, displays included World War I, Japanese memorabilia associated with the World War II attack on Pearl Harbor, USMC in the South Pacific, D-Day landings, German Third Reich, Korean War and 8063rd M.A.S.H memorabilia.
* Burt Reynolds and Friends Museum, Jupiter
* Civil War Soldiers Museum, Pensacola
* Children's Science Center N. Ft. Myers. Closed in 2005.
* Cuban Museum of Arts and Culture, Miami
* Dinosaur Wildlife, Spring Hill, open from 1962 to 1998
* Dow Museum of Historic Houses, |St. Augustine, closed in 2014 and turned into a bed-and-breakfast complex
* Florida International Museum at St. Petersburg College, St. Petersburg, closed in 2010
* Florida Military Aviation Museum, St. Petersburg, closed in about 2007
* Flying Tigers Warbird Restoration Museum, Kissimmee, closed in 2004
* Fred Bear Museum, Gainesville, showcased archery & the contribution of Fred Bear, a man who popularized the sport, closed in 2003, current museum located in Springfield, Missouri
* Gulfcoast Wonder & Imagination Zone, The Hands-On Science Museum, Sarasota
* Gulf Coast Museum of Art, Largo, closed January 2009, collection now part of Florida International Museum at St. Petersburg College
* Klassix Auto Attraction, Daytona Beach, closed in 2003
* Hibel Museum of Art, Jupiter
* Jacksonville Maritime Museum, Jacksonville, Florida, included large scale models of ships from the Mayflower to present day vessels, paintings, photographs and maritime artifacts
* Longboat Key Center for the Arts, closed in May 2017
* Mark K. Wheeler Gallery, Fort Lauderdale, Gallery of the former Art Institute of Fort Lauderdale
* Museum of the Sea and Indian, Destin, destroyed by hurricane in 1995
* Museum of Weapons & Early American History, St. Augustine
* Panama Canal Museum Florida, Seminole, closed in 2012, collections transferred to the George A. Smathers Libraries at the University of Florida in Gainesville
* Skatelab, Atlantic Beach, closed in 2009, original store and museum in Simi Valley, California still open
* Teddy Bear Museum of Naples, Naples, closed in 2005
* Tom Gaskins Cypress Knee Museum, Palmdale, featured carved cypress knees, closed in 2000
* Tragedy in U.S. History Museum, St. Augustine, featured articles and memorabilia related to tragic events, closed in 1998
* Turtle Kraals Museum, Key West
* USS Requin, Tampa, now part of the Carnegie Science Center in Pittsburgh, Pennsylvania
* Welaka Maritime Museum, Welaka
* Wings of Freedom Aviation Museum, Florida, Dunnellon
* World Chess Hall of Fame and Sidney Samole Museum, moved to St. Louis, Missouri in 2011

## Planned museums

* Bay of Pigs Museum, Miami
* Holocaust Documentation & Education Center, Dania Beach
* John H. Fetterman Maritime Museum and Research Center, Pensacola, website
* Mark X. and E. Louise Benson Museum, Gulf Breeze, website
* Pulse Memorial and Museum, Orlando