# List of bus routes in Melbourne

This is a list of bus routes that are part of the bus network, in Melbourne, Australia, which is overseen by Public Transport Victoria.
Kinetic Melbourne operate approximately 30% of the bus network, signing contracts in 2021 as part of the Melbourne Metropolitan Bus Franchise. The remaining 70% of the network is operated under 23 contracts with other bus operators, all of these contracts were entered into in mid-2018 and will expire in 2025 or 2028.

## Route numbering scheme

The route number of buses in Melbourne can tell a little about the route's operator, area served and even its history.
Route numbering was a reflection of the designated zonal area used during the 1980s. Areas were referred to as "Neighbourhood Zones". However, as part of the "Grow or Go" policies, route numbers across the bus network were affected. There are no two-digit route numbers – these are used exclusively by trams.
* 150–199, 400–899: These routes are run by private companies. Most are found in the middle and outer suburbs, where they provide feeders to railway stations and provide local travel.
* 200–399: These routes were former government-run routes (Melbourne & Metropolitan Tramways Board and then MetBus). They are found mostly in inner suburbs such as Footscray/Sunshine, Elsternwick-Brighton, Clifton Hill and Port Melbourne. Routes in the Doncaster/Templestowe area also have these numbers due to a Tramways board takeover after the area's private operator collapsed. In the 1990s all MetBus routes were put out to private franchise. Until August 2013, these services were operated by Melbourne Bus Link and National Bus Company. All of these services were taken over by Transdev Melbourne in August 2013, and then taken over by Kinetic Melbourne in January 2022.
* 900 series: Historically these route numbers were used by Victorian Railways, who ran buses co-ordinated with trains on some routes. Routes with these numbers have either been abolished or incorporated into other routes. The 900 series is now used for the high frequency SmartBus services, which are mostly cross-town and orbital routes.

## By route number
### 100–199

| Route | Destinations | Operator(s) | Notes |
| 109 | Queen Bridge Street tram stop Station Pier | Ventura Bus Lines | Supplementary service to tram route 109 when cruise ships are docked at Station Pier |
| 150 | Williams Landing station via Sayers Road Tarneit station | CDC Melbourne | Operates all night on Friday & Saturday nights as part of the Night Network |
| 151 | Williams Landing station via Westmeadows Lane Tarneit station | CDC Melbourne | |
| 152 | Williams Landing station via Palmers Road Tarneit station | CDC Melbourne | Commenced 30 May 2021 |
| 153 | Williams Landing station via Princes Highway Werribee station | CDC Melbourne | |
| 160 | Hoppers Crossing station via Morris Road Tarneit station | CDC Melbourne | |
| 161 | Hoppers Crossing station via Pacific Werribee Werribee station | CDC Melbourne | |
| 166 | Hoppers Crossing station via Pacific Werribee Wyndham Vale station | CDC Melbourne | |
| 167 | Hoppers Crossing station via Pacific Werribee Tarneit station | CDC Melbourne | |
| 170 | Werribee station via Pacific Werribee Tarneit station | CDC Melbourne | |
| 180 | Werribee station via Tarneit Road Tarneit station | CDC Melbourne | Operates all night on Friday & Saturday nights as part of the Night Network |
| 181 | Werribee station via Pacific Werribee Hoppers Crossing station | CDC Melbourne | |
| 182 | Werribee station via Tarneit West Tarneit Station | CDC Melbourne | Commenced 30 May 2021 |
| 190 | Werribee station via Ballan Road Wyndham Vale station | CDC Melbourne | Operates all night on Friday & Saturday nights as part of the Night Network |
| 191 | Werribee station via Wyndham Vale Station Manor Lakes | CDC Melbourne | |
| 192 | Werribee station via Black Forest Road Wyndham Vale station | CDC Melbourne | |

### 200–299

| Route | Image | Destinations | Operator(s) | Notes |
| 200 | | Queen Street City via Kew Junction Bulleen | Kinetic Melbourne | |
| 201 | | Box Hill station Deakin University | Ventura Bus Lines | Only operates when Deakin University Burwood is open. |
| 202 | | Yarra Bend via Johnston Street Melbourne University | Kinetic Melbourne | Commenced 20 September 2021, limited stops service, weekdays and some public holidays only. |
| 207 | | Queen Street City via Doncaster Park & Ride Westfield Doncaster | Kinetic Melbourne | Operates all night on Friday & Saturday nights between Queen Street City & Box Hill station as part of the Night Network |
| 215 | | Caroline Springs Highpoint Shopping Centre | Kinetic Melbourne | |
| 216 | | Sunshine via Footscray City - Queen & Collins Streets | Kinetic Melbourne | Formerly ran between Sunshine & Brighton Beach until 16 November 2019 |
| 220 | | Sunshine via Footscray, Queen Victoria Market City - Queen & Collins Streets | Kinetic Melbourne | Formerly ran between Sunshine & Gardenvale until 16 November 2019 |
| 223 | | Yarraville via Footscray Highpoint Shopping Centre | Kinetic Melbourne | |
| 232 | | Altona NorthQueen Victoria Market | Kinetic Melbourne | |
| 234 | | Garden CityQueen Victoria Market | Kinetic Melbourne | |
| 235 | | Southern Cross stationFishermans Bend | Kinetic Melbourne | |
| 236 | | Garden CityQueen Victoria Market | Kinetic Melbourne | |
| 237 | | Southern Cross stationFishermans Bend | Kinetic Melbourne | |
| 246 | | Elsternwick station via St Kilda Clifton Hill station | Kinetic Melbourne | |
| 250 | | CityLa Trobe University, Bundoora | Kinetic Melbourne | Operates all night on Friday & Saturday nights as part of the Night Network |
| 251 | | CityNorthland Shopping Centre | Kinetic Melbourne | |
| 270 | | Box Hill station via Blackburn North Mitcham | Kinetic Melbourne | |
| 271 | | Box Hill station via Park Orchards Ringwood | Kinetic Melbourne | |
| 273 | Travelling east along Springvale Road | The Pines Shopping Centre via Donvale Nunawading | Kinetic Melbourne | |
| 279 | | Box Hill CentralWestfield Doncaster Templestowe | Kinetic Melbourne | |
| 280 | | The Pines Shopping CentreWestfield DoncasterStockland The Pines | Kinetic Melbourne | Clockwise circular service |
| 281 | | Templestowe via Westfield Doncaster Box Hill station | Kinetic Melbourne | Route truncated from Deakin University to Box Hill station from 16 October, 2023 |
| 282 | | The Pines Shopping CentreWestfield DoncasterThe Pines Shopping Centre | Kinetic Melbourne | Anti-clockwise circular service |
| 284 | | Box Hill station via Mont Albert Doncaster Park & Ride | Kinetic Melbourne | Route truncated from Westfield Doncaster to Doncaster Park & Ride |
| 285 | | Doncaster Park & Ride via Balwyn Camberwell | Kinetic Melbourne | Route truncated from Westfield Doncaster to Doncaster Park & Ride |
| 293 | | Box Hill station via Westfield Doncaster Greensborough | Kinetic Melbourne | |
| 295 | | Westfield DoncasterThe Pines Shopping Centre | Kinetic Melbourne | Route truncated from Box Hill station to Westfield Doncaster |

### 300–399

| Route | Destinations | Operator(s) | Notes |
| 301 | ReservoirLa Trobe University (Bundoora Campus) | Dysons | University Shuttle Bus |
| 302 | City via Kew East Box Hill station | Kinetic Melbourne | Originally had an extension to Crown Melbourne |
| 303 | CityRingwood North | Kinetic Melbourne | Ceased 27 July 2014 Reinstated 13 October 2014 |
| 304 | City via Eastern Freeway Westfield Doncaster | Kinetic Melbourne | |
| 305 | City via Bulleen Terminates at Westfield Doncaster during off-peak The Pines Shopping Centre | Kinetic Melbourne | Originally ran to Warrandyte Bridge |
| 309 | City via Eastern Freeway The Pines Shopping Centre | Kinetic Melbourne | |
| 318 | CityDeep Creek | Kinetic Melbourne | |
| 343 | Hurstbridge station via Diamond Creek station Greensborough station | Panorama Coaches | |
| 350 | City via Eastern Freeway La Trobe University, Bundoora | Kinetic Melbourne | |
| 356 | Epping stationWollert East | Dysons | |
| 357 | Wollert WestThomastown station | Dysons | Operates all night on Friday & Saturday nights as part of the Night Network |
| 358 | EppingWollert | Dysons | |
| 364 | WarrandyteRingwood | Kinetic Melbourne | |
| 370 | MitchamRingwood | Kinetic Melbourne | |
| 381 | Diamond Creek stationMernda station | Dysons | |
| 382 | WhittleseaNorthland SC | Dysons | |
| 383 | PalisadesUniversity Hill | Dysons | |
| 384 | KinglakeWhittlesea | Dysons | |
| 385 | Whittlesea / Mernda stationGreensborough station | Dysons | |
| 386 | Mernda stationBundoora RMIT | Dysons | Operates all night on Friday & Saturday nights as part of the Night Network |
| 387 | Mernda stationBundoora RMIT | Dysons | |
| 388 | Mernda Loop | Dysons | Anti-clockwise circular service |
| 389 | Mernda Loop | Dysons | Clockwise circular service |
| 390 | MerndaCraigieburn | Dysons | Commenced 31 October 2021 |

### 400–499

| Route | Destinations | Operator(s) | Notes |
| 400 | SunshineLaverton station | CDC Melbourne Transit Systems Victoria | Known as the "Prison Bus", as it services Port Phillip Prison, the Metropolitan Remand Centre and the Dame Phyllis Frost Centre |
| 401 | North Melbourne station via Royal Melbourne HospitalUniversity of Melbourne | Transit Systems Victoria | Melbourne's highest service frequency for any bus route, operates on weekdays only |
| 402 | Footscray via Kensington station East Melbourne | Transit Systems Victoria | |
| 403 | FootscrayUniversity of Melbourne | Transit Systems Victoria | |
| 404 | Footscray via Newmarket Moonee Ponds Junction | Transit Systems Victoria | |
| 406 | Footscray via Highpoint Shopping Centre Keilor East | CDC Melbourne | Operates all night on Friday & Saturday nights as part of the Night Network |
| 407 | Highpoint Shopping CentreAvondale Heights | CDC Melbourne | |
| 408 | Highpoint Shopping Centre via Sunshine St Albans | CDC Melbourne | |
| 409 | Highpoint Shopping Centre via Footscray Yarraville | CDC Melbourne | Formerly Yarraville to West Footscray |
| 410 | FootscraySunshine | CDC Melbourne Transit Systems Victoria (Friday & Saturday Nights only) | Operates all night on Friday & Saturday nights as part of the Night Network |
| 411 | Footscray via Millers Rd, Altona North Laverton station | CDC Melbourne | |
| 412 | Footscray via Mills St, Altona North Laverton station | CDC Melbourne | |
| 414 | Footscray via Geelong Road Laverton station | CDC Melbourne | |
| 415 | Laverton station via Altona Williamstown | CDC Melbourne | |
| 417 | Laverton stationLaverton North | CDC Melbourne | |
| 418 | St AlbansCaroline Springs | CDC Melbourne | |
| 419 | St Albans via Taylors Lake Watergardens Town Centre | CDC Melbourne | |
| 420 | Sunshine via Deer Park Watergardens Town Centre | Transit Systems Victoria | Operates all night on Friday & Saturday nights as part of Night Network |
| 421 | St Albans via Keilor Downs Watergardens Town Centre | CDC Melbourne | |
| 422 | Sunshine via Deer Park Brimbank Central Shopping Centre | Transit Systems Victoria | |
| 423 | St Albans via Cairnlea Brimbank Central Shopping Centre | CDC Melbourne | |
| 424 | St Albans via Albanvale Brimbank Central Shopping Centre | CDC Melbourne | |
| 425 | St AlbansWatergardens Town Centre | CDC Melbourne | |
| 426 | Sunshine via Albion Caroline Springs | Kinetic Melbourne | |
| 427 | SunshineSunshine West | Transit Systems Victoria | |
| 428 | SunshineSunshine West | Transit Systems Victoria | |
| 429 | Sunshine/Sunshine South Loop | Kinetic Melbourne | Commenced 17 November 2019 to partly replace route 219 |
| 431 | YarravilleKingsville | Transit Systems Victoria | |
| 432 | Newport via Altona Gate Shopping Centre Yarraville | Transit Systems Victoria | Formerly Newport to Paisley |
| 433 | Darley, Victoria via Bacchus Marsh station Hillview Estate | Bacchus Marsh Coaches | |
| 434 | Bacchus Marsh station Telford Park | Bacchus Marsh Coaches | |
| 439 | WerribeeWerribee South | CDC Melbourne | Diverts via Werribee Park Mansion and Werribee Open Range Zoo during their opening hours |
| 441 | WerribeeWestleigh Gardens | CDC Melbourne | |
| 443 | WerribeeSouth Ring Road | CDC Melbourne | Circular service |
| 444 | Rockbank stationAintree | Transit Systems Victoria | |
| 452 | Melton stationEynesbury | Bacchus Marsh Coaches | |
| 453 | Melton via Brookfield Melton station | Transit Systems Victoria | |
| 454 | Melton stationCobblebank station | Transit Systems Victoria | |
| 455 | Melton station via Woodgrove Shopping Centre Micasa Rise | Transit Systems Victoria | |
| 456 | Sunshine via Caroline Springs Melton (Woodgrove Shopping Centre) | Transit Systems Victoria | |
| 457 | Melton via Melton West Melton station | Transit Systems Victoria | |
| 458 | Melton station via Woodgrove Shopping Centre Kurunjang | Transit Systems Victoria | |
| 459 | Melton station via Melton South Arnolds Creek | Transit Systems Victoria | |
| 460 | Watergardens Town CentreCaroline Springs | Kastoria Bus Lines | |
| 461 | Watergardens Town CentreCaroline Springs | CDC Melbourne | |
| 462 | Watergardens Town CentreCaroline Springs | Kastoria Bus Lines | |
| 463 | Watergardens Town CentreHillside | Kastoria Bus Lines | Commenced 12 July 2020 to replace part of route 476 |
| 465 | Essendon via Keilor East Keilor Park | Ryan Brothers Bus Service | |
| 467 | AberfeldieMoonee Ponds | Ryan Brothers Bus Service | |
| 468 | EssendonHighpoint Shopping Centre (Maribyrnong) | Ryan Brothers Bus Service | |
| 469 | Moonee Ponds JunctionKeilor East | Kastoria Bus Lines | Commenced 12 July 2020 to replace routes 475 and 501 |
| 471 | Williamstown via Newport, Altona Gate Shopping Centre Sunshine | Transit Systems Victoria | |
| 472 | Williamstown via Footscray Moonee Ponds Junction | Transit Systems Victoria | |
| 473 | Gisborne stationGisborne | Gisborne Transit | |
| 474 | Gisborne stationGisborne | Gisborne Transit | On-Demand Service |
| 475 | SunburyDiggers Rest | Sunbury Bus Service | Commenced 24 March 2024 |
| 476 | Moonee Ponds Junction via Keilor Watergardens Town Centre | Kastoria Bus Lines | Previously operated to Hillside via Taylors Lakes until 11 July 2020. Deleted section replaced by route 463. |
| 477 | Moonee Ponds Junction via Westfield Airport West Broadmeadows | CDC Melbourne | |
| 478 | Westfield Airport WestMelbourne Airport | CDC Melbourne | Operated to Moonee Ponds Junction until 27 July 2014 |
| 479 | Westfield Airport West via Melbourne Airport Sunbury | CDC Melbourne | Previously operated to Moonee Ponds Junction & to the city on weekends. Both ceased 27 July 2014 |
| 481 | SunburySunbury (Mount Lion) | Sunbury Bus Service | |
| 482 | Westfield Airport WestMelbourne Airport | CDC Melbourne | Operates during peak hours only |
| 483 | Sunbury via Diggers Rest Moonee Ponds Junction | Sunbury Bus Service | |
| 484 | BroadmeadowsRoxburgh Park | CDC Melbourne | |
| 485 | SunburySunbury (Wilsons Lane) | Sunbury Bus Service | |
| 486 | SunburySunbury (Rolling Meadows) | Sunbury Bus Service | |
| 487 | SunburySunbury (Killara Heights) | Sunbury Bus Service | |
| 488 | SunburySunbury (Jacksons Hill) | Sunbury Bus Service | |
| 489 | SunburySunbury (Canterbury Hills) | Sunbury Bus Service | |
| 490 | Westfield Airport WestGowanbrae | CDC Melbourne | On demand bus service for part of the route, requires passengers to book 15 mins in advance from designated bus stops. |
| 494 | Williams Landing station via Alamanda Bvd Point Cook South | CDC Melbourne | |
| 495 | Williams Landing station via Broadwalk Boulevard Point Cook South | CDC Melbourne | |
| 496 | Laverton station via Sanctuary Lakes Shopping Centre Sanctuary Lakes | CDC Melbourne | Commenced 28 April 2013 as a service to Sanctuary Lakes Shopping Centre. Extended to Sanctuary Lakes as part of the introduction of the Regional Rail Link. |
| 497 | Williams Landing station via Sanctuary Lakes Shopping Centre Saltwater Estate | CDC Melbourne | |
| 498 | Laverton station via Dunnings Rd Hoppers Crossing station | CDC Melbourne | Commenced 21 June 2015 as part of the introduction of the Regional Rail Link |

### 500–599

| Route | Destinations | Operator(s) | Notes |
| 501 | Donnybrook via Hume Freeway Craigieburn | Broadmeadows Bus Service | Commenced 7 January 2024 |
| 503 | Essendon via Brunswick Brunswick East | Dysons | Operated by Moonee Valley Coaches until March 2024 |
| 504 | Moonee Ponds Junction via Brunswick East Clifton Hill | Dysons | |
| 505 | Moonee Ponds Junction via Parkville Gardens Melbourne University | Dysons | |
| 506 | Moonee Ponds Junction via Brunswick Westgarth | Dysons | Operated by Moonee Valley Coaches until March 2024 |
| 508 | Moonee Ponds Junction via Northcote, Brunswick Alphington | Dysons | |
| 509 | Barkly Square Brunswick West | Dysons | Discontinued 29 September 2012. Reinstated in 2016 |
| 510 | Essendon via Northcote, Brunswick, Thornbury Ivanhoe | Kinetic Melbourne | Operated by MorelandBus until October 2023 |
| 511 | Donnybrook station via Olivine Estate Mandalay Circuit | Broadmeadows Bus Service | |
| 512 | Strathmore via Coburg Coburg East | Kinetic Melbourne | Operated by MorelandBus until October 2023 |
| 513 | Eltham via Lower Plenty Glenroy | Dysons | Formerly alternated running via either Greensborough or Lower Plenty until 2 July 2022, altered to operate via Lower Plenty only |
| 514 | Eltham via Greensborough Glenroy | Dysons | Commenced 3 July 2022, replacing the Greensborough deviation of route 513 |
| 517 | Northland Shopping Centre via Greensborough, View Bank St Helena | Dysons | |
| 518 | Greensborough via St Helena St Helena West | Dysons | |
| 525 | Donnybrook via Mickleham Craigieburn | Dysons | Commenced 22 December 2019 |
| 526 | CoburgPreston West | Ventura Bus Lines | |
| 527 | Northland Shopping Centre via Coburg Gowrie station (Glenroy) | Ventura Bus Lines | |
| 528 | Craigieburn station Craigieburn Central Shopping Centre | Broadmeadows Bus Service | |
| 529 | CraigieburnCraigieburn North | Broadmeadows Bus Service | |
| 530 | Coburg via Gowrie station, Fawkner and Coburg North Campbellfield | Broadmeadows Bus Service | |
| 531 | Coburg North via Somerset Estate Upfield | Broadmeadows Bus Service | |
| 532 | Craigieburn via Upfield Broadmeadows | Broadmeadows Bus Service | |
| 533 | Craigieburn via Craigieburn Craigieburn North | Broadmeadows Bus Service | |
| 534 | Glenroy (Coburg) | Dysons | |
| 536 | GlenroyGowrie station (Glenroy) | Dysons | |
| 537 | Craigieburn station Craigieburn West | Broadmeadows Bus Service | |
| 538 | Broadmeadows(Somerset Estate) | Dysons | |
| 540 | Broadmeadows via Coolaroo Upfield | Dysons | |
| 541 | Broadmeadows via Meadow Heights Craigeburn North | Dysons | |
| 542 | Roxburgh Park via Meadow Heights, Broadmeadows, Glenroy, Oak Park Pascoe Vale | Dysons | |
| 543 | Greenvale GardensRoxburgh Park | CDC Melbourne | |
| 544 | Craigieburn North via Roxburgh Park Roxburgh Park | Broadmeadows Bus Service | |
| 546 | Heidelberg via Clifton Hill Melbourne UniversityQueen Victoria Market | Dysons | |
| 548 | Kew via Ivanhoe La Trobe University, Bundoora | Ventura Bus Lines | |
| 549 | IvanhoeNorthland Shopping Centre | Ventura Bus Lines | |
| 550 | Northland Shopping CentreLa Trobe University, Bundoora | Ventura Bus Lines | |
| 551 | HeidelbergLa Trobe University, Bundoora | Ventura Bus Lines | |
| 552 | Reservoir North EastNorthcote Plaza | Dysons | |
| 553 | PrestonPreston West | Dysons | |
| 554 | ThomastownThomastown | Dysons | Clockwise loop via West Lalor |
| 555 | Epping via Lalor, Thomastown, Reservoir Northland Shopping Centre | Dysons | |
| 556 | Epping via Keon Park Northland Shopping Centre | Dysons | |
| 557 | Thomastown via Lalor Thomastown | Dysons | Anti-clockwise loop via West Lalor |
| 558 | ReservoirReservoir | Dysons | Circular Route via Reservoir North West |
| 559 | Thomastown via Lalor Thomastown | Dysons | Circular Route via Lalor Plaza Shopping Centre |
| 561 | Pascoe Vale via La Trobe University Macleod | Dysons | |
| 564 | South Morang station via Mill Park Stables Shopping Centre RMIT, Bundoora | Dysons | |
| 566 | Northland Shopping Centre via Bundoora, Greensborough Lalor | Dysons | |
| 567 | Northcote via Northland Shopping Centre Regent station | Dysons | |
| 569 | Pacific Epping via Mill Park South Morang station | Dysons | |
| 570 | Thomastown via Lalor Plaza Shopping Centre RMIT, Bundoora | Dysons | |
| 577 | South Morang via Epping station Pacific Epping | Dysons | |
| 578 | Eltham via Kangaroo Ground – Warrandyte Road Warrandyte | Panorama Coaches | |
| 579 | Eltham via Research – Warrandyte Road Warrandyte | Panorama Coaches | |
| 580 | Eltham via Ryans Road Diamond Creek | Panorama Coaches | |
| 582 | Eltham via Main Rd Eltham | Panorama Coaches | Circular Route via Eucalyptus Road |

### 600–699

| Route | Destinations | Operator(s) | Notes |
| 600 | St Kilda Light Rail station Westfield Southland | Kinetic Melbourne | |
| 601 | Huntingdale station Monash University, Clayton campus | CDC Melbourne | University Shuttle Bus Operates on weekdays only |
| 603 | Brighton Beach via Elsternwick station Burnley station | Kinetic Melbourne | Commenced 17 November 2019 between Alfred Hospital and Brighton Beach, replacing 216 Altered to Burnley on 17 November 2024 |
| 604 | Elsternwick station via Orrong Road Anzac Station | Kinetic Melbourne | Commenced 17 November 2019 between Alfred Hospital and Gardenvale, replacing 220 Altered to run between Anzac and Elsternwick on 17 November 2014 |
| 605 | City Queen Street via Kooyong Road Gardenvale | CDC Melbourne | |
| 606 | Elsternwick via St Kilda Fishermans Bend | CDC Melbourne | |
| 609 | Fairfield via Royal Talbot Hospital & Kew Hawthorn | Dysons | |
| 612 | Box Hill station via Camberwell, Glen Iris Chadstone Shopping Centre | CDC Melbourne | |
| 623 | Glen Waverley St Kilda | CDC Melbourne | |
| 624 | Kew via Carnegie or Darling station Oakleigh | CDC Melbourne | |
| 625 | Elsternwick Chadstone | CDC Melbourne | |
| 626 | Middle Brighton Chadstone | CDC Melbourne | |
| 627 | Moorabbin Chadstone | Ventura Bus Lines | Commenced 16 June 2019, original route number for routes 625 & 626 |
| 630 | Elwood Monash University | CDC Melbourne | Operates all night on Friday & Saturday nights as part of the Night Network |
| 631 | Waverley Gardens Shopping Centre via Monash University Westfield Southland | Ventura Bus Lines | |
| 663 | Belgrave via Monbulk, Silvan and Mt Evelyn Lilydale | Ventura Bus Lines | |
| 664 | Westfield Knox via Croydon and Bayswater Chirnside Park | Ventura Bus Lines | |
| 668 | Ringwood via Croydon Hills Croydon | Kinetic Melbourne | |
| 669 | Ringwood via Ringwood East Croydon | Kinetic Melbourne | |
| 670 | Ringwood via Croydon Lilydale | Ventura Bus Lines | Operates all night on Friday & Saturday nights as part of the Night Network |
| 671 | Chirnside Park Croydon | Ventura Bus Lines | |
| 672 | Croydon via Wonga Park Chirnside Park | Ventura Bus Lines | |
| 675 | Chirnside Park Mooroolbark | Ventura Bus Lines | |
| 677 | Lilydale via The Gateway Chirnside Park | Ventura Bus Lines | |
| 679 | Chirnside Park via Lilydale and Montrose Ringwood | Ventura Bus Lines | |
| 680 | Lilydale Mooroolbark | Ventura Bus Lines | |
| 681 | Westfield Knox via Rowville & Lysterfield Westfield Knox | Ventura Bus Lines | Loop service, on a clockwise route |
| 682 | Westfield Knox via Rowville & Lysterfield Westfield Knox | Ventura Bus Lines | Loop service, on an anti-clockwise route |
| 683 | Chirnside Park Warburton | Martyrs Bus Service | Country fares apply from Woori Yallock |
| 684 | Ringwood station via Healesville & Marysville Eildon | McKenzie's Tourist Services | Country fares apply from Healesville, minimal services. Truncated from Southern Cross station to Ringwood station in November 2023 |
| 685 | Lilydale station via Coldstream & Yarra Glen Healesville Sanctuary | McKenzie's Tourist Services | Truncated from Chirnside Park to Lilydale station in November 2023 |
| 688 | Croydon via Mount Dandenong Upper Ferntree Gully | Ventura Bus Lines | |
| 689 | Croydon Montrose | Ventura Bus Lines | |
| 690 | Croydon via Kilsyth Boronia | Ventura Bus Lines | |
| 691 | Boronia via Ferntree Gully & Rowville Waverley Gardens Shopping Centre | Ventura Bus Lines | |
| 693 | Belgrave Oakleigh | Ventura Bus Lines | Operates all night on Friday & Saturday nights between Oakleigh station & Scoresby as part of the Night Network |
| 694 | Belgrave via Olinda Mt. Dandenong | Ventura Bus Lines | |
| 695 | Belgrave Gembrook | Ventura Bus Lines | |
| 695F | Westfield Fountain Gate Gembrook | Ventura Bus Lines | Operates on Friday nights & weekends only, one service operates between Emerald & Dandenong Market on Tuesdays only |
| 696 | Olinda via Olinda Monbulk Road Monbulk | Ventura Bus Lines | |
| 697 | Belgrave Belgrave South | Ventura Bus Lines | One service extends to Dandenong Market on Tuesdays only and to Westfield Fountain Gate on Thursdays only |
| 699 | Belgrave Upwey | Ventura Bus Lines | |

### 700–799

| Route | Destinations | Operator(s) | Notes |
| 701 | Oakleigh Bentleigh | Ventura Bus Lines | |
| 703 | Blackburn Middle Brighton | Ventura Bus Lines | SmartBus route, operates all night on Friday & Saturday nights as part of the Night Network |
| 704 | Westall station Oakleigh | Ventura Bus Lines | Previously ran to East Clayton until 15 May 2020 |
| 705 | Mordialloc via Braeside & Clayton South Springvale | Ventura Bus Lines | |
| 706 | Mordialloc via Aspendale & Edithvale Chelsea | Ventura Bus Lines | |
| 708 | Carrum via Westfield Southland Hampton | Ventura Bus Lines | |
| 709 | Mordialloc via Governor Road Noble Park station | Ventura Bus Lines | |
| 732 | Box Hill station via Vermont South Tram Terminus Upper Ferntree Gully | Ventura Bus Lines | Services to/from Westfield Knox connect with tram route 75 to/from the City at Vermont South. |
| 733 | Box Hill station Oakleigh | Ventura Bus Lines | |
| 734 | Glen Iris Glen Waverley | Ventura Bus Lines | |
| 735 | Box Hill station via K-Mart Plaza, Forest Hill Chase Shopping Centre Nunawading | Ventura Bus Lines | |
| 736 | Mitcham via Glen Waverley Blackburn | Ventura Bus Lines | |
| 737 | Croydon station via Boronia Station, Westfield Knox, Glen Waverley station Monash University, Clayton campus | Ventura Bus Lines | Some services run via deviations through Bayswater and Croydon. |
| 738 | Mitcham station via Knox Private Hospital, Wantirna College Westfield Knox | Ventura Bus Lines | |
| 740 | Mitcham station via Reserve Avenue, Churinga Avenue Vermont East | Ventura Bus Lines | One service starts from Vermont Secondary College each weekday. |
| 742 | Ringwood station via Vermont South, Glen Waverley station, Oakleigh Station Chadstone Shopping Centre | Ventura Bus Lines | Some early morning and late evening services are shortened. Some services do not utilise the Monash University deviation. Route formerly terminated at Eastland Shopping Centre until 10 April 2021. |
| 745A | Westfield Knox via Victoria Road and Sasses Avenue Bayswater station | Ventura Bus Lines | |
| 745B | Bayswater station Via Victoria Road and Sasses Avenue Boronia station | Ventura Bus Lines | |
| 745C | Bayswater station Via Victoria Road and Sasses Avenue Wantirna Primary School | Ventura Bus Lines | |
| 745D | Bayswater station Via Mountain Highway and Stud Road Wantirna Primary School | Ventura Bus Lines | |
| 753 | Bayswater via Boronia Glen Waverley | Ventura Bus Lines | Only limited services run the full route to Bayswater, any services commence or terminate at Boronia |
| 754 | Glen Waverley via Wheelers Hill Rowville | Ventura Bus Lines | During the morning peak, some services run express to Glen Waverley, during the evening peak, some services run express to Rowville |
| 755 | Bayswater via The Basin & Ferntree Gully Westfield Knox | Ventura Bus Lines | Some weekday off-peak services terminate/commence at The Basin, Westfield Knox bound buses serve Ferntree Gully station, Bayswater bound buses do not |
| 757 | Westfield Knox Scoresby | Ventura Bus Lines | |
| 758 | Westfield Knox Knoxfield Shops | Ventura Bus Lines | |
| 760 | Cranbourne via Carrum Downs Seaford | Ventura Bus Lines | |
| 765 | Box Hill station via Blackburn Mitcham | Ventura Bus Lines | |
| 766 | Box Hill station Burwood | Ventura Bus Lines | |
| 767 | Westfield Southland via Deakin University Box Hill station | Ventura Bus Lines | |
| 770 | Frankston via Ashleigh Avenue Karingal Hub Shopping Centre | Ventura Bus Lines | |
| 771 | Frankston via McMahons Road or Orwil Street Langwarrin | Ventura Bus Lines | Service alternates between travelling down Orwil Street (which return to Frankston station) and McMahons Road |
| 772 | Frankston Eliza Heights | Ventura Bus Lines | Loop service, which returns to Frankston via Delacombe Park |
| 773 | Frankston Frankston South | Ventura Bus Lines | Loop service, returning to Frankston, on Saturday, the bus runs to Frankston South via an alternate route, returning via the normal route |
| 774 | Frankston Delacombe Park | Ventura Bus Lines | Loop Service, which returns to Frankston via a different route |
| 775 | Frankston Lakewood | Ventura Bus Lines | |
| 776 | Frankston Pearcedale | Ventura Bus Lines | Loop Service, returning to Frankston, the route is Clockwise in the morning, and anti-clockwise in the afternoon |
| 777 | Karingal Hub Shopping Centre McClelland Drive, Langwarrin | Ventura Bus Lines | |
| 778 | Kananook station Carrum Downs | Ventura Bus Lines | |
| 779 | Frankston Belvedere | Ventura Bus Lines | |
| 780 | Frankston Carrum | Ventura Bus Lines | |
| 781 | Frankston via Mornington Dromana | Ventura Bus Lines | Extended from Mount Martha to Dromana on 20 February 2022 |
| 782 | Frankston via Hastings Flinders | Ventura Bus Lines | Country fares apply from Sandy Point Road to Flinders |
| 783 | Frankston Hastings | Ventura Bus Lines | |
| 784 | Frankston via Mornington Osborne | Ventura Bus Lines | |
| 785 | Frankston Mornington East | Ventura Bus Lines | |
| 786 | Rye St Andrews Beach | Ventura Bus Lines | Country fares apply |
| 787 | Sorrento Rosebud | Ventura Bus Lines | Country fares apply, truncated to Rosebud Plaza on 20 February 2022 |
| 788 | Frankston Portsea | Ventura Bus Lines | Country fares apply, operates all night on Friday & Saturday nights between Sorrento & Frankston station as part of the Night Network |
| 789 | Langwarrin via Langwarrin North Frankston | Cranbourne Transit | Truncated to Langwarrin from 13 November 2016, reviously ran to Cranbourne West |
| 790 | Langwarrin via Langwarrin South Frankston | Cranbourne Transit | Truncated to Langwarrin from 13 November 2016, previously ran to Cranbourne West |
| 791 | Cranbourne Frankston | Cranbourne Transit | |
| 792 | CranbournePearcedale | Cranbourne Transit | Introduced 13 November 2016 |
| 795 | Cranbourne Warneet | Cranbourne Transit | |
| 796 | Cranbourne Clyde | Cranbourne Transit | |
| 798 | Cranbourne Selandra Rise Estate | Cranbourne Transit | |
| 799 | Merinda Park station The Avenue Village Shopping Centre | Cranbourne Transit | |

### 800–899

| Route | Destinations | Operator(s) | Notes |
| 800 | Dandenong via Springvale and Oakleigh Chadstone Shopping Centre | Ventura Bus Lines | |
| 802 | Dandenong via Mulgrave and Oakleigh Chadstone Shopping Centre | Ventura Bus Lines | |
| 804 | Dandenong via Wheelers Hill and Oakleigh Chadstone Shopping Centre | Ventura Bus Lines | |
| 811 | Dandenong via Springvale and Westfield Southland Brighton | Ventura Bus Lines | |
| 812 | Dandenong via Parkmore and Westfield Southland Brighton Terminates at Braeside on evenings & weekends | Ventura Bus Lines | |
| 813 | Dandenong via Parkmore and Springvale Waverley Gardens Shopping Centre | Ventura Bus Lines | |
| 814 | Dandenong via Waverley Gardens Shopping Centre Springvale South | Ventura Bus Lines | |
| 816 | Keysborough South Noble Park | Ventura Bus Lines | Commenced 28 November 2021 |
| 822 | Chadstone Shopping Centre via Westfield Southland Sandringham | Ventura Bus Lines | |
| 823 | North Brighton Westfield Southland | Ventura Bus Lines | |
| 824 | Moorabbin via Clayton Keysborough | Ventura Bus Lines | |
| 825 | Moorabbin via Mentone Westfield Southland | Ventura Bus Lines | |
| 828 | Hampton via Dandenong Berwick | Ventura Bus Lines | |
| 831 | Kingsmere Estate via Casey Hospital Berwick station | Ventura Bus Lines | |
| 832 | Frankston Carrum Downs | Ventura Bus Lines | |
| 833 | Frankston Carrum station | Ventura Bus Lines | Operates all night on Friday & Saturday nights as part of the Night Network |
| 834 | Berwick via Narre Warren Berwick | Ventura Bus Lines | Loop service, runs anti-clockwise |
| 835 | Berwick via Narre Warren Berwick | Ventura Bus Lines | Loop service, runs clockwise |
| 836 | Eden Rise Shopping Centre via Bridgewater Estate Berwick station | Ventura Bus Lines | |
| 837 | Berwick station Beaconsfield East | Ventura Bus Lines | |
| 838 | Emerald via Beaconsfield Westfield Fountain Gate | Ventura Bus Lines | |
| 839 | Berwick station North Berwick | Ventura Bus Lines | |
| 840 | Gembrook Pakenham | Ventura Bus Lines | Formerly Narre Warren – Berwick Circle |
| 841 | Narre Warren North Cranbourne | Ventura Bus Lines | |
| 842 | Endeavour Hills Westfield Fountain Gate | Ventura Bus Lines | |
| 843 | Dandenong via Daniel Solander Drive Endeavour Hills | Ventura Bus Lines | |
| 844 | Dandenong Doveton | Ventura Bus Lines | |
| 845 | Dandenong via Kennington Park Drive Endeavour Hills | Ventura Bus Lines | |
| 846 | Eden Rise Shopping Centre via Bryn Mawr Boulevard Berwick station | Ventura Bus Lines | |
| 847 | The Avenue Village Shopping Centre Berwick station | Ventura Bus Lines | |
| 848 | Dandenong Brandon Park Shopping Centre | Ventura Bus Lines | |
| 850 | Dandenong via Waverley Gardens Shopping Centre Glen Waverley | Ventura Bus Lines | |
| 857 | Chelsea via Patterson Lakes Dandenong | Ventura Bus Lines | |
| 858 | Edithvale via Chelsea Aspendale Gardens | Ventura Bus Lines | |
| 861 | Dandenong via Dandenong Hospital Endeavour Hills | Ventura Bus Lines | |
| 862 | Dandenong Chadstone Shopping Centre | Ventura Bus Lines | |
| 863 | Endeavour Hills Hampton Park Shopping Centre | Ventura Bus Lines | |
| 881 | Merinda Park station Clyde North | Cranbourne Transit | Commenced 30 January 2022 |
| 885 | Glen Waverley Springvale | Ventura Bus Lines | |
| 887 | Frankston station via Monash University, Peninsula campus Rosebud | Ventura Bus Lines | |
| 888 | Berwick station Clyde | Ventura Bus Lines | Commenced 28 March 2021 |
| 889 | Berwick station Clyde North | Ventura Bus Lines | Commenced 28 March 2021 |
| 890 | Lynbrook station Dandenong station | Cranbourne Transit | |
| 891 | Lynbrook station Westfield Fountain Gate | Cranbourne Transit | |
| 892 | Narre Warren South Dandenong | Cranbourne Transit | |
| 893 | Cranbourne via Hampton Park Dandenong | Cranbourne Transit | |
| 894 | Amberley Park (Narre Warren South) Hallam station | Cranbourne Transit | |
| 895 | Narre Warren South via Hampton Park Westfield Fountain Gate | Cranbourne Transit | |
| 897 | Lynbrook station Clyde | Cranbourne Transit | |
| 898 | Cranbourne Archers Field Drive (Cranbourne East) | Cranbourne Transit | |
| 899 | The Avenue Village Shopping Centre Berwick station | Ventura Bus Lines | |

### 900–999

| Route | Destinations | Operator(s) | Notes |
| 900 | Stud Park Shopping Centre via Huntingdale, Oakleigh, Chadstone Shopping Centre Caulfield | Ventura Bus LinesCDC Melbourne | SmartBus route, operates all night on Friday & Saturday nights between Stud Park Shopping Centre & Oakleigh as part of the Night Network |
| 901 | Frankston station via Dandenong, Westfield Knox, Ringwood, Blackburn, The Pines, Greensborough, South Morang, Epping and Broadmeadows Melbourne Airport | Kinetic Melbourne | SmartBus route, operates all night on Friday & Saturday nights between Dandenong & Ringwood as part of the Night Network |
| 902 | Chelsea station via Springvale, Glen Waverley, Nunawading, Doncaster, Eltham, Greensborough, Keon Park, Broadmeadows Westfield Airport West | Kinetic Melbourne | SmartBus route |
| 903 | Altona station via Sunshine, Essendon, Northland Shopping Centre, Heidelberg, Doncaster, Box Hill station, Chadstone Shopping Centre, Oakleigh, Mentone Mordialloc Centreway Shopping Centre | Kinetic Melbourne | SmartBus route |
| 905 | City via Eastern Freeway, Templestowe The Pines Shopping Centre | Kinetic Melbourne | SmartBus route, operates all night on Friday & Saturday nights as part of the Night Network |
| 906 | City via Eastern Freeway, The Pines Shopping Centre Warrandyte Bridge | Kinetic Melbourne | SmartBus route |
| 907 | City via Eastern Freeway, Doncaster Mitcham station | Kinetic Melbourne | SmartBus route, operates all night on Friday & Saturday nights as part of the Night Network |
| 908 | City via Eastern Freeway, Templestowe Lower Terminates at Doncaster Park & Ride during off-peak The Pines Shopping Centre | Kinetic Melbourne | SmartBus route, operates all night on Friday & Saturday nights as part of the Night Network |
| 922 | Westfield Southland via North Brighton St Kilda Light Rail station | Kinetic Melbourne | |
| 923 | Westfield Southland via Brighton Beach station St Kilda Light Rail station | Kinetic Melbourne | |
| 925 | Pakenham Town Service via Lakeside | Ventura Bus Lines | Loop service |
| 926 | Westfield Fountain Gate Pakenham | Ventura Bus Lines | |
| 927 | Pakenham Town Service via Meadowvale Village | Ventura Bus Lines | Loop service |
| 928 | Pakenham Cardinia Road station | Ventura Bus Lines | |
| 929 | Pakenham Town Service via Army Road | Ventura Bus Lines | Loop service |
| 941 | Sunshine station via Keilor DownsWatergardens station | Transit Systems Victoria | Operates all night on Friday & Saturday nights as part of the Night Network |
| 943 | Watergardens station via Caroline SpringsMelton | Transit Systems Victoria | Operates all night on Friday & Saturday nights as part of the Night Network |
| 947 | Footscray station Newport | Transit Systems Victoria | Operates all night on Friday & Saturday nights as part of the Night Network |
| 949 | Williams Landing via Point CookAltona Meadows | Transit Systems Victoria | Operates all night on Friday & Saturday nights as part of the Night Network |
| 951 | Brunswick station via Brunswick West & Pascoe ValeGlenroy railway station | Ventura Bus Lines | Operates all night on Friday & Saturday nights as part of the Night Network |
| 953 | Broadmeadows railway station via Meadow Heights & Roxburgh ParkCraigieburn railway station | Ventura Bus Lines | Operates all night on Friday & Saturday nights as part of the Night Network |
| 959 | City via Airport West & NiddrieBroadmeadows railway station | Ventura Bus Lines | Operates all night on Friday & Saturday nights as part of the Night Network |
| 965 | Lilydale railway station via Woori Yallock & HealesvilleLilydale railway station | McKenzie's Tourist Services | Operates all night on Friday & Saturday nights as part of the Night Network |
| 967 | Glen Waverley railway station via Knox CityCroydon station | Ventura Bus Lines | Returns via Kilsyth & Boronia, operates all night on Friday & Saturday nights as part of the Night Network |
| 978 | Clayton station via MulgraveDandenong railway station | Ventura Bus Lines | Operates all night on Friday & Saturday nights as part of the Night Network |
| 979 | Clayton station via KeysboroughDandenong railway station | Ventura Bus Lines | Operates all night on Friday & Saturday nights as part of the Night Network |
| 981 | Dandenong railway station via Berwick & Narre Warren SouthCranbourne railway station | Ventura Bus Lines | Operates all night on Friday & Saturday nights as part of the Night Network |
| 982 | Dandenong railway station via Endeavour Hills & Hampton ParkCranbourne railway station | Ventura Bus Lines | Operates all night on Friday & Saturday nights as part of the Night Network |

### Airports

| Destinations | Operator(s) | Notes |
| City (Southern Cross station) Melbourne Airport Express Service | SkyBus | |
| City (Southern Cross station) via Werribee RSL Avalon Airport | SkyBus | |
| Frankston Melbourne Airport | SkyBus | Peninsula Express via 12 dedicated intermediate stops, including St Kilda and Brighton |

### Former Routes

| Route | Destinations | Operator(s) | Notes |
| 201 | City via Kew Junction Westfield Doncaster | Transdev Melbourne | Ceased 27 July 2014, replaced by route 304 |
| 202 | Box Hill station via Balwyn Kew East | Transdev Melbourne | Ceased 27 July 2014, incorporated into route 302 |
| 203 | City via Eastern Freeway Bulleen | Transdev Melbourne | Ceased 27 July 2014 |
| 205 | Melbourne University via Kew Junction Westfield Doncaster | Transdev Melbourne | Ceased 27 July 2014 |
| 219 | Sunshine South via Footscray, City Gardenvale | Transdev Melbourne | Ceased 16 November 2019, replaced by Routes 216, 429 & 603 |
| 238 | City(Port Melbourne) | Transdev Melbourne | Ceased 27 July 2014 |
| 249 | CityLa Trobe University, Bundoora | National Bus Company | Route Scrapped |
| 253 | Garden City (Port Melbourne) via City North Carlton | Transdev Melbourne | Ceased 27 July 2014, replaced by route 236 |
| 283 | Westfield DoncasterBulleen | National Bus Company | Incorporated into routes 280/282 on 24 November 2008 |
| 286 | Box Hill station via Blackburn The Pines Shopping Centre | Transdev Melbourne | Ceased 27 July 2014, partly replaced by route 271 |
| 289 | Box Hill stationDonvale | National Bus Company | Incorporated into routes 280/282, 281 & 305 on 24 November 2008 |
| 291 | Box Hill station via Westfield Doncaster Heidelberg | National Bus Company | Incorporated into route 903 on 20 April 2009 |
| 301 | City via Templestowe The Pines Shopping Centre | National Bus Company | Replaced by route 905 on 4 October 2010 |
| 306 | City via Doncaster East, Canopus Drive Ringwood North | National Bus Company | Incorporated into route 303 on 4 October 2010 |
| 307 | City via Donvale Mitcham | National Bus Company | Replaced by route 907 on 4 October 2010 |
| 313 | CityDoncaster Park & Ride | Transdev Melbourne | Ceased 27 July 2014 |
| 315 | CityBox Hill station | Transdev Melbourne | Ceased 27 July 2014 |
| 316 | CityDeep Creek | National Bus Company | Ceased on 4 October 2010 |
| 340 | City via Thornbury La Trobe University, Bundoora | Transdev Melbourne | Ceased 27 July 2014, replaced by route 350 |
| 365 | Ringwood via Tunstall Square Westfield Doncaster | National Bus Company | Incorporated into routes 271, 280 & 282 on 24 November 2008 |
| 366 | Ringwood via Croydon Hills Croydon | Transdev Melbourne | Ceased 27 July 2014, replaced by route 380 |
| 367 | Ringwood via Ringwood East Croydon | Transdev Melbourne | Ceased 27 July 2014, replaced by route 380 |
| 380 | Ringwood Croydon | Kinetic Melbourne | Loop service replacing routes 366 and 367, ceased 9 November 2024, split into routes 668 and 669 |
| 413 | Laverton via Hoppers Crossing Pacific Werribee | CDC Melbourne Operated by CDC Melbourne – Altona Depot | Scrapped 28 April 2013, partly replaced by routes 493, 494 & 496 |
| 416 | LavertonHoppers Crossing | CDC Melbourne Operated by CDC Melbourne – Altona Depot | Scrapped 28 April 2013, partly replaced by routes 493, 495 & 496 |
| 422 | St AlbansDelahey | CDC Melbourne Operated by CDC Melbourne – Sunshine Depot | Scrapped 27 July 2014, replaced by route 425 |
| 429 | YarravilleAltona East | Sita Buslines | Incorporated into route 432 on 30 June 2008 |
| 430 | YarravilleGeelong Road, Yarraville | Sita Buslines | Circular route, replaced by routes 431 & 432 on 30 June 2008 |
| 433 | NewportGeelong Road, Brooklyn | Sita Buslines | Incorporated into route 471 |
| 438 | WerribeeManor Lakes | Westrans | Replaced by routes 447, 448 & 449 on 19 April 2010 |
| 440 | Werribee via Wyndham Vale, Pacific Werribee Hoppers Crossing | Westrans | Circular route, replaced by routes 446, 448 & 449 on 19 April 2010 |
| 442 | Hoppers Crossing via Pacific Werribee, The Grange Tarneit | CDC Melbourne | Ceased 21 June 2015 |
| 447 | Werribee via Ballan Road Manor Lakes | CDC Melbourne | Ceased 21 June 2015 |
| 448 | Pacific Werribee via Greaves Street North Manor Lakes | CDC Melbourne | Ceased 21 June 2015 |
| 449 | Werribee via Black Forest Road Manor Lakes | CDC Melbourne | Ceased 21 June 2015 |
| 451 | SunshineBrimbank Central Shopping Centre | Sita Buslines | Scrapped 27 July 2014, replaced by routes 420 & 423 |
| 454 | SunshineSunshine West | Sita Buslines | Scrapped 27 July 2014, replaced by routes 427 & 428 |
| 475 | Moonee Ponds Junction via Niddrie Keilor East | Kastoria Bus Lines | Ceased 11 July 2020, replaced by route 469 |
| 493 | Williams Landing station via Point Cook Town Centre East Werribee | CDC Melbourne | Commenced 28 April 2013, ceased 21 June 2015 |
| 500 | Broadmeadows via Melbourne Airport Sunbury | Tullamarine Bus Lines | Scrapped 2014 |
| 501 | Moonee Ponds Junction via Strathmore Niddrie | Kastoria Bus Lines | Ceased 11 July 2020, replaced by route 469 |
| 520 | Doreen via Yarrambat Greensborough | Dysons | |
| 547 | KewLa Trobe University, Bundoora | Ivanhoe Bus Company | Section incorporated into route 548 |
| 560 | Greensborough via Bundoora, Keon Park Broadmeadows | East West Bus Company | Incorporated into route 902 on 5 April 2010 |
| 562 | Whittlesea via South Morang station, Mill Park, Bundoora Northland Shopping Centre | Dysons | Ceased 24 July 2016, replaced by route 382 |
| 565 | GreensboroughGreensborough North East | Dysons | Incorporated into route 563 |
| 565 | Kinglake via Humevale Whittlesea | Dysons | Ceased 24 July 2016, replaced by route 384 |
| 568 | Northland Shopping CentreNorth WatsoniaGreensborough | Dysons | Incorporated into route 566 |
| 571 | EppingSouth Morang | East West Bus Company | Trainlink service. Initially operated from South Morang to Campbellfield (Hume Highway). Extended to Roxburgh Park station in October 2007, replacing route 571A. Truncated to Epping and partly replaced by extension of SmartBus 901 in September 2010. Ceased 22 April 2012 with the opening of the South Morang rail extension |
| 571A | Roxburgh ParkEpping | East West Bus Company | Incorporated into route 571 in October 2007 with the opening of Roxburgh Park station |
| 572 | University Hill via Mill Park Stables Shopping Centre, South Morang station, Mernda Doreen | Dysons (Ceased from July 2016) | Ceased 24 July 2016 |
| 573 | University Hill Mill Park Lakes/Palisades | Dysons | Ceased 24 July 2016 |
| 581 | ElthamResearch | Panorama Coaches | Shortworking of routes 578 & 579 |
| 615 | Box Hill stationBalwyn North | Quince's Scenicruisers | Incorporated into route 284 |
| 627 | Elsternwick Brighton East | Driver Bus Lines | Ceased 25 September 2010 and split and simplified into route 625 Elsternwick – Chadstone via Oakleigh and 626 Middle Brighton – Chadstone Via Carnegie |
| 634 | Middle BrightonLilydale | Quince's Scenicruisers | Split up between: route 703 (Middle Brighton – Monash University section), route 631 (Monash University – Waverley Gardens Shopping Centre section), route 691 (Waverley Gardens – Boronia section), route 737 (Boronia – Croydon section), route 664 (Croydon – Chirnside Park/Lilydale section) |
| 665 | RingwoodDandenong | Invicta Bus Services | Incorporated into route 901 |
| 673 | Lillydale Lakes Lillydale Lakes | Ventura Bus Lines | Circular route, ceased 28 January 2022 |
| 676 | Lilydale via Lilydale East Lilydale | Ventura Bus Lines | Circular route, ceased 2 October 2021 |
| 686 | Healesville via Badger Creek & Healesville Sanctuary Healesville | McKenzie's Tourist Services | Loop service, ceased November 24 2023 |
| 687 | Healesville via Chum Creek & Mount Lebanon Healesville | McKenzie's Tourist Services | Loop service, ceased November 24 2023 |
| 700 | Box Hill stationMordialloc | Ventura Bus Lines | SmartBus route, incorporated into route 903 on 20 April 2009, Mordialloc – Chelsea extension reverted to route 706 |
| 731 | CamberwellBalwyn North | Quince's Scenicruisers | Incorporated into route 285 |
| 768 | Box Hill station Deakin University | Ventura Bus Lines | Operated on University days only, ceased October 2023 |
| 769 | Karingal Hub Shopping Centre Frankston | Peninsula Bus Lines | Sunday service only, incorporated into routes 770, 771, 777 |
| 794 | CranbournePearcedale | Cranbourne Transit | Route scrapped |
| 797 | Cranbourne Town Service | Cranbourne Transit | Loop service in Cranbourne, limited services, ceased 13 November 2016 |
| 801 | DandenongKeysborough | Grenda's Bus Services | Scrapped |
| 815 | Dandenong Noble Park | Ventura Bus Lines | Ceased 27 November 2021 |
| 821 | Westfield Southland Clayton | Ventura Bus Lines | Ceased 1 December 2023 |
| 826 | Hampton via Westfield Southland, Westfield Fountain Gate Pakenham | Grenda's Bus Services | Incorporated into route 828 between Hampton & Berwick, replaced by Route 926 between Westfield Fountain Gate & Pakenham |
| 830 | DandenongFrankston | Grenda's Bus Services | Incorporated into routes 832, 833 & 901 |
| 831 | DandenongFrankston | Grenda's Bus Services | Incorporated into routes 832, 833 & 901 |
| 849 | Dandenong via Gleneagles Dr Mossgiel Park | Ventura Bus Lines | Ceased 30 May 2020 |
| 886 | Rosebud Chisholm TAFE – Rosebud Campus | Ventura Bus Lines | Ceased 18 February 2022 |
| 888 | Nunawading via Edithvale Chelsea | Grenda's Bus Services | SmartBus route, incorporated into route 902 on 5 April 2010, Edithvale section replaced by route 858 |
| 889 | Nunawading via Chelsea Heights Chelsea | Grenda's Bus Services | SmartBus route, incorporated into route 902 on 5 April 2010, Chelsea Heights section replaced by route 858 |
| 896 | Cranbourne station Cranbourne East | Cranbourne Transit | TrainLink service, ceased 13 November 2016 |

## By bus company

| Operator | Routes | Notes |
| Broadmeadows Bus Service | 501, 511, 528, 529, 530, 531, 532, 533, 537, 544 | Owned by Northern Transit Holdings |
| Cranbourne Transit | 789, 790, 791, 792, 795, 796, 798, 799, 881, 890, 891, 892, 893, 894, 895, 897, 898 | Formerly known as FA Phillips Bus Service. Owned by Pulitano Group. |
| CDC Melbourne | 150, 151, 153, 160, 161, 166, 167, 170, 180, 181, 182, 190, 191, 192, 400, 406, 407, 408, 409, 410, 411, 412, 414, 415, 417, 418, 419, 421, 423, 424, 425, 439, 441, 443, 461, 477, 478, 479, 482, 484, 490, 494, 495, 496, 497, 498, 543, 601, 605, 606, 612, 623, 624, 625, 626, 630, 900 | Subsidiary of ComfortDelGro Australia |
| Dysons | 301, 356, 357, 358, 381, 382, 383, 384, 385, 386, 387, 388, 389, 503, 504, 505, 506, 508, 509, 513, 517, 518, 534, 536, 538, 540, 541, 542, 546, 552, 553, 554, 555, 556, 557, 558, 559, 561, 564, 566, 567, 569, 570, 577, 609 Ruthven Secondary College – 001,002,003,004,005 Merrilands Express. | |
| Kastoria Bus Lines | 460, 462, 463, 469, 476 | Owned by Northern Transit Holdings |
| Kinetic Melbourne | 200, 202, 207, 215, 216, 219, 220, 223, 232, 234, 235, 236, 237, 246, 250, 251, 270, 271, 273, 279, 280, 281, 282, 284, 285, 293, 295, 302, 303, 304, 305, 309, 318, 350, 364, 370, 426, 510, 512, 600, 603, 604, 668, 669, 901, 902, 903, 905, 906, 907, 908, 922, 923 | |
| Martyrs Bus Service | 683 | Owned by Dineen Group |
| McKenzie's Tourist Services | 684, 685, 686, 687 | Owned by Dineen Group |
| Panorama Coaches | 343, 578, 579, 580, 582 | Owned by Dineen Group |
| Ryan Brothers Bus Service | 465, 467, 468 | |
| SkyBus | Melbourne Airport – City, Avalon Airport - City, Melbourne Airport – Frankston | |
| Sunbury Bus Service | 475, 481, 483, 485, 486, 487, 488, 489 | Owned by Donric Group |
| Transit Systems Victoria | 400, 401, 402, 403, 404, 420, 422, 427, 428, 431, 432, 453, 455, 456, 457, 458, 459, 471, 472 | Owned by Transit Systems. Formerly known as Sita Buslines. |
| Ventura Bus Lines | 526, 527, 548, 549, 550, 551, 631, 663, 664, 670, 671, 672, 673, 675, 676, 677, 679, 680, 681, 682, 688, 689, 691, 693, 690, 694, 695, 696, 697, 699, 701, 703, 704, 705, 706, 708, 709, 732, 733, 734, 735, 736, 737, 738, 740, 742, 745, 753, 754, 755, 757, 758, 765, 766, 767, 770, 771, 772, 773, 774, 775, 776, 777, 778, 779, 780, 781, 782, 783, 784, 785, 786, 787, 788, 800, 802, 804, 811, 812, 813, 814, 816, 821, 822, 823, 824, 825, 828, 831, 832, 833, 834, 835, 836, 837, 838, 839, 841, 842, 843, 844, 845, 846, 847, 848, 849, 850, 857, 858, 861, 862, 863, 885, 886, 887, 888, 889, 899, 900, 925, 926, 927, 928, 929, 966, 968 | |