# List of Victorian state by-elections

This is a list of by-elections for the Victorian Legislative Assembly. A by-election may be held when a member's seat becomes vacant through resignation, death or some other reasons. These are referred to as casual vacancies.
* Brackets around a date (D/M/Y) indicate that the candidate was unopposed when nominations closed or that, as a result of an appeal against an election result, the sitting member was replaced by the appellant. These candidates were declared "elected unopposed" with effect from the date of the closing of nominations or appeal decision, and there was no need to hold a by-election.
* By-elections which resulted in a change in party representation are highlighted as: Gains for the Labor Party and its splinter groups in red; for the Liberal Party and its predecessors in blue; for the National Party and its predecessors in green; for the Greens in green and for independents and minor parties in grey.
60th Legislative Assembly (2022–2026) :
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Werribee | Tim Pallas | | Labor | Resigned | 8 February 2025 | John Lister | | Labor |
| Prahran | Sam Hibbins | | Independent | Resigned from parliament after leaving Greens | 8 February 2025 | Rachel Westaway | | Liberal |
| Mulgrave | Daniel Andrews | | Labor | Resigned as Premier | 18 November 2023 | Eden Foster | | Labor |
| Warrandyte | Ryan Smith | | Liberal | Resigned | 26 August 2023 | Nicole Werner | | Liberal |
| Narracan | Gary Blackwood | | Liberal | Supplementary election due to death of a candidate at the 2022 election. | 28 January 2023 | Wayne Farnham | | Liberal |
| 59th Legislative Assembly (2018–2022) |||||||||
| No by-elections held |||||||||
| 58th Legislative Assembly (2014–2018) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Northcote | Fiona Richardson | | Labor | Died | 18 November 2017 | Lidia Thorpe | | Greens |
| South-West Coast | Denis Napthine | | Liberal | Resigned | 31 October 2015 | Roma Britnell | | Liberal |
| Polwarth | Terry Mulder | | Liberal | Resigned | 31 October 2015 | Richard Riordan | | Liberal |
| Gippsland South | Peter Ryan | | National | Resigned | 14 March 2015 | Danny O'Brien | | National |
| 57th Legislative Assembly (2010–2014) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Lyndhurst | Tim Holding | | Labor | Resigned | 27 April 2013 | Martin Pakula | | Labor |
| Melbourne | Bronwyn Pike | | Labor | Resigned | 21 July 2012 | Jennifer Kanis | | Labor |
| Niddrie | Rob Hulls | | Labor | Resigned | 24 March 2012 | Ben Carroll | | Labor |
| Broadmeadows | John Brumby | | Labor | Resigned following election loss | 19 February 2011 | Frank McGuire | | Labor |
| 56th Legislative Assembly (2006–2010) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Altona | Lynne Kosky | | Labor | Resigned | 13 February 2010 | Jill Hennessy | | Labor |
| Kororoit | Andre Haermeyer | | Labor | Resigned | 28 June 2008 | Marlene Kairouz | | Labor |
| Williamstown | Steve Bracks | | Labor | Resigned as Premier | 15 September 2007 | Wade Noonan | | Labor |
| Albert Park | John Thwaites | | Labor | Resigned as Deputy Premier | 15 September 2007 | Martin Foley | | Labor |
| 55th Legislative Assembly (2002–2006) |||||||||
| No by-elections held |||||||||
| 54th Legislative Assembly (1999–2002) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Benalla | Pat McNamara | | National | Resigned following election loss | 13 May 2000 | Denise Allen | | Labor |
| Burwood | Jeff Kennett | | Liberal | Resigned following election loss | 11 December 1999 | Bob Stensholt | | Labor |
| Frankston East | Peter McLellan | | Independent | Supplementary election due to death of the sitting member at the 1999 election. | 16 October 1999 | Matt Viney | | Labor |
| 53rd Legislative Assembly (1996–1999) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Northcote | Tony Sheehan | | Labor | Resigned | 15 August 1998 | Mary Delahunty | | Labor |
| Mitcham | Roger Pescott | | Liberal | Resigned | 13 December 1997 | Tony Robinson | | Labor |
| Gippsland West | Alan Brown | | Liberal | Resigned | 1 February 1997 | Susan Davies | | Independent |
| 52nd Legislative Assembly (1992–1996) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Williamstown | Joan Kirner | | Labor | Resigned | 13 August 1994 | Steve Bracks | | Labor |
| Coburg | Tom Roper | | Labor | Resigned | 14 May 1994 | Carlo Carli | | Labor |
| Broadmeadows | Jim Kennan | | Labor | Resigned | 18 September 1993 | John Brumby | | Labor |
| 51st Legislative Assembly (1988–1992) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Shepparton | Peter Ross-Edwards | | National | Resigned | 19 October 1991 | Don Kilgour | | National |
| Thomastown | Beth Gleeson | | Labor | Died | 3 February 1990 | Peter Batchelor | | Labor |
| Greensborough | Pauline Toner | | Labor | Resigned due to ill health (cancer) | 15 April 1989 | Sherryl Garbutt | | Labor |
| Rodney | Eddie Hann | | National | Resigned | 4 March 1989 | Noel Maughan | | National |
| 50th Legislative Assembly (1985–1988) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Ballarat North | Tom Evans | | Liberal | Resigned | 23 July 1988 | Steve Elder | | Liberal |
| Kew | Prue Sibree | | Liberal | Resigned | 19 March 1988 | Jan Wade | | Liberal |
| 49th Legislative Assembly (1982–1985) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Swan Hill | Alan Wood | | Liberal | Resigned | 7 May 1983 | Barry Steggall | | National |
| Warrnambool | Ian Smith | | Liberal | Resigned in unsuccessful attempt to win Liberal preselection for the 1983 Wannon federal by-election even though he would have been required to resign if he had been successful at the preselection. | 7 May 1983 | Adam Kempton | | Liberal |
| Springvale | Kevin King | | Labor | Died | 19 March 1983 | Eddie Micallef | | Labor |
| Malvern | Lindsay Thompson | | Liberal | Resigned following defeat as Premier | 4 December 1982 | Geoff Leigh | | Liberal |
| Keilor | Jack Ginifer | | Labor | Resigned due to ill health | 17 July 1982 | George Seitz | | Labor |
| 48th Legislative Assembly (1979–1982) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Kew | Rupert Hamer | | Liberal | Resigned following resignation as Premier | 15 August 1981 | Prue Sibree | | Liberal |
| Morwell | Derek Amos | | Labor | Resigned due to ill health | 27 June 1981 | Valerie Callister | | Labor |
| 47th Legislative Assembly (1976–1979) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Richmond | Clyde Holding | | Labor | Resigned to enter federal politics at the 1977 federal election | 17 December 1977 | Theo Sidiropoulos | | Labor |
| Melbourne | Barry Jones | | Labor | Resigned to enter federal politics at the 1977 federal election | 17 December 1977 | Keith Remington | | Labor |
| Greensborough | Monte Vale | | Liberal | Died | 5 November 1977 | Pauline Toner | | Labor |
| 46th Legislative Assembly (1973–1976) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Brunswick East | David Bornstein | | Labor | Resigned | 12 April 1975 | Ron McAlister | | Labor |
| Greensborough | Monte Vale | | Liberal | Result overturned by the Court of Disputed Returns | 13 October 1973 | Monte Vale | | Liberal |
| 45th Legislative Assembly (1970–1973) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Hampden | Henry Bolte | | Liberal | Resigned as Premier | 7 October 1972 | Tom Austin | | Liberal |
| Melbourne | Arthur Clarey | | Labor | Died | 9 June 1972 | Barry Jones | | Labor |
| Gisborne | Julian Doyle | | Liberal | Resigned | 11 December 1971 | Athol Guy | | Liberal |
| Kew | Arthur Rylah | | Liberal | Resigned due to ill health | 17 April 1971 | Rupert Hamer | | Liberal |
| 44th Legislative Assembly (1967–1970) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Dandenong | Len Reid | | Liberal | Resigned to enter federal politics at the 1969 federal election | 6 December 1969 | Alan Lind | | Labor |
| Swan Hill | Harold Stirling | | Country | Died | 14 September 1968 | Henry Broad | | Country |
| 43rd Legislative Assembly (1964–1967) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Grant | Roy Crick | | Labor | Died | 8 October 1966 | Jack Ginifer | | Labor |
| Caulfield | Alexander Fraser | | Liberal | Died | 18 September 1965 | Ian McLaren | | Liberal |
| 42nd Legislative Assembly (1961–1964) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Mildura | Nathaniel Barclay | | Country | Died | 27 October 1962 | Milton Whiting | | Country |
| Broadmeadows | Harry Kane | | Liberal and Country | Died | 4 August 1962 | John Wilton | | Labor |
| Richmond | Bill Towers | | Labor | Died | 12 May 1962 | Clyde Holding | | Labor |
| 41st Legislative Assembly (1958–1961) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Ballarat North | Russell White | | Country | Resigned to take up position on the Trotting Control Board | 12 November 1960 | Tom Evans | | Liberal and Country |
| Scoresby | George Knox | | Liberal and Country | Died | 17 September 1960 | Bill Borthwick | | Liberal and Country |
| Footscray | Ernie Shepherd | | Labor | Died | 8 October 1958 | Bill Divers | | Labor |
| 40th Legislative Assembly (1955–1958) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Northcote | John Cain | | Labor | Died | 21 September 1957 | Frank Wilkes | | Labor |
| Camberwell | Robert Whately | | Liberal and Country | Died in a car accident | 21 April 1956 | Vernon Wilcox | | Liberal and Country |
| Mornington | William Leggatt | | Liberal and Country | Resigned to take up appointment as Agent-General for Victoria in London | 3 March 1956 | Roberts Dunstan | | Liberal and Country |
| Flemington | Jack Holland | | Labor | Died | 18 February 1956 | Kevin Holland | | Labor |
| 39th Legislative Assembly (1952–1955) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Malvern | Trevor Oldham | | Liberal and Country | Died | 11 July 1953 | John Bloomfield | | Liberal and Country |
| 38th Legislative Assembly (1950–1952) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Toorak | Edward Reynolds | | Liberal and Country | Resigned | 13 September 1952 | Horace Petty | | Liberal and Country |
| Port Melbourne | Tom Corrigan | | Labor | Died | 13 September 1952 | Stan Corrigan | | Labor |
| Prahran | Frank Crean | | Labor | Resigned to enter federal politics at the 1951 federal election | 16 June 1951 | Robert Pettiona | | Labor |
| Ivanhoe | Rupert Curnow | | Liberal and Country | Died | 24 February 1951 | Frank Block | | Liberal and Country |
| 37th Legislative Assembly (1947–1950) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Clifton Hill | Jack Cremean | | Labor | Resigned to enter federal politics at the 1949 federal election | 17 December 1949 | Joseph O'Carroll | | Labor |
| Kew | Wilfrid Kent Hughes | | Liberal and Country | Resigned to enter federal politics at the 1949 federal election | 17 December 1949 | Arthur Rylah | | Liberal and Country |
| Richmond | Stan Keon | | Labor | Resigned to enter federal politics at the 1949 federal election | 17 December 1949 | Frank Scully | | Labor |
| Brunswick | James Jewell | | Labor | Died | 16 July 1949 | Peter Randles | | Labor |
| Prahran | Bill Quirk | | Labor | Died | 22 January 1949 | Frank Crean | | Labor |
| Geelong | Fanny Brownbill | | Labor | Died | 13 November 1948 | Edward Montgomery | | Liberal |
| Toorak | Robert Bell Hamilton | | Liberal | Died | 19 June 1948 | Edward Reynolds | | Liberal |
| 36th Legislative Assembly (1945–1947) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Collingwood | Tom Tunnecliffe | | Labor | Resigned | 20 September 1947 | Bill Towers | | Labor |
| Benambra | Roy Paton | | Country | Died | 7 June 1947 | Thomas Mitchell | | Country |
| 35th Legislative Assembly (1943–1945) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Prahran | John Ellis | | United Australia | Died | 18 August 1945 | Bill Quirk | | Labor |
| Clifton Hill | Herbert Cremean | | Labor | Died | 7 July 1945 | Jack Cremean | | Labor |
| Bendigo | Arthur Cook | | Labor | Died | 26 May 1945 | Bill Galvin | | Labor |
| Lowan | Hamilton Lamb | | Country | Died in a Japanese prisoner of war camp on the Burma Railway | 4 November 1944 | Wilfred Mibus | | Country |
| Bulla and Dalhousie | Reginald James | | Country | Died | 27 September 1944 | Leslie Webster | | Country |
| Rodney | William Dunstone | | Country | Died | 12 April 1944 | Richard Brose | | Country |
| Waranga | Ernest Coyle | | Country | Died | 9 October 1943 | Wollaston Heily | | Country |
| Nunawading | Ivy Weber | | Independent | Resigned in unsuccessful attempt to enter federal politics at the 1943 federal election | 4 September 1943 | Bob Gray | | Labor |
| 34th Legislative Assembly (1940–1943) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Maryborough and Daylesford | George Frost | | Labor | Died | 28 November 1942 | Clive Stoneham | | Labor |
| Gippsland North | Alexander Borthwick | | Country | Died | 20 June 1942 | Bill Fulton | | Country |
| Port Melbourne | James Murphy | | Labor | Died | 18 April 1942 | Tom Corrigan | | Labor |
| Toorak | Stanley Argyle | | United Australia | Died | 11 January 1941 | Harold Thonemann | | United Australia |
| Polwarth | Allan McDonald | | United Australia | Resigned to enter federal politics at the 1940 federal election | 2 Nov 1940 | Edward Guye | | Country |
| Coburg | Frank Keane | | Labor | Died | 13 July 1940 | Charlie Mutton | | Independent Labor |
| 33rd Legislative Assembly (1937–1940) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Hawthorn | John Austin Gray | | United Australia | Died | 10 June 1939 | Les Tyack | | United Australia |
| Gippsland North | James Weir McLachlan | | Independent | Died | 5 November 1938 | Alexander Borthwick | | Country |
| Geelong | William Brownbill | | Labor | Died | 4 June 1938 | Fanny Brownbill | | Labor |
| Ouyen | Albert Bussau | | Country | Resigned to take up appointment as Agent-General for Victoria in London | 5 May 1938 | Keith Dodgshun | | Country |
| 32nd Legislative Assembly (1935–1937) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Benalla | Edward Cleary | | Country | Died | 3 October 1936 | Frederick Cook | | Independent |
| Goulburn Valley | Murray Bourchier | | Country | Resigned to take up appointment as Agent-General for Victoria in London | 19 September 1936 | John MacDonald | | Country |
| Rodney | John Allan | | Country | Died | 18 April 1936 | William Dunstone | | Country |
| Allandale | Thomas Parkin | | United Australia | Died | 21 March 1936 | Patrick Denigan | | Labor |
| 31st Legislative Assembly (1932–1935) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Nunawading | Robert Menzies | | United Australia | Resigned to enter federal politics at the 1934 federal election | 1 September 1934 | William Boyland | | United Australia |
| Clifton Hill | Maurice Blackburn | | Labor | Resigned to enter federal politics at the 1934 federal election | 20 August 1934 | Herbert Cremean | | Labor |
| Gunbower | Henry Angus | | United Australia | Died | 12 May 1934 | Norman Martin | | Independent Country |
| Allandale | Alexander Peacock | | United Australia | Died | 11 November 1933 | Millie Peacock | | United Australia |
| Warrnambool | James Fairbairn | | United Australia | Resigned to contest the 1933 Flinders federal by-election | 11 November 1933 | Keith McGarvie | | United Australia |
| Polwarth | James McDonald | | United Australia | Died | 16 September 1933 | Allan McDonald | | United Australia |
| Boroondara | Richard Linton | | United Australia | Resigned to take up appointment as Agent-General for Victoria in London | 29 April 1933 | Trevor Oldham | | United Australia |
| Benambra | Henry Beardmore | | United Australia | Died | 15 October 1932 | Roy Paton | | Country |
| Carlton | Robert Solly | | Labor | Died | 9 July 1932 | Bill Barry | | Labor |
| 30th Legislative Assembly (1929–1932) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Caulfield | Frederick Forrest | | Australian Liberal | Died | 22 November 1930 | Harold Daniel Luxton | | Nationalist |
| Hawthorn | William Murray McPherson | | Nationalist | Resigned | 27 September 1930 | John Austin Gray | | Nationalist |
| 29th Legislative Assembly (1927–1929) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Gippsland West | Arthur Walter | | Country | Resigned in unsuccessful bid for the federal seat of Indi at the 1929 election | 19 October 1929 | Matthew Bennett | | Country |
| Barwon | Edward Morley | | Nationalist | Died | 6 July 1929 | Thomas Maltby | | Nationalist |
| Castlemaine and Kyneton | Harry Lawson | | Nationalist | Resigned to contest a Senate seat at the 1929 federal election | 2 February 1929 | Walter Langslow | | Nationalist |
| Brighton | Oswald Snowball | | Nationalist | Died | 28 April 1928 | Ian MacFarlan | | Nationalist |
| Gippsland South | Henry Bodman | | Independent | Died | 3 December 1927 | Walter West | | Nationalist |
| 28th Legislative Assembly (1924–1927) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Flemington | Edward Warde | | Labor | Died | 9 December 1925 | Jack Holland | | Labor |
| Fitzroy | John Billson | | Labor | Died | 4 February 1925 | Maurice Blackburn | | Labor |
| Glenelg | William Thomas | | Labor | Died | 14 August 1924 | Ernie Bond | | Labor |
| 27th Legislative Assembly (1921–1924) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Gippsland West | Sir John Mackey | | Nationalist | Died | 23 May 1924 | Arthur Walter | | VFU |
| Dalhousie | Allan Cameron | | Nationalist | Died | 31 January 1924 | Reg Pollard | | Labor |
| Daylesford | Donald McLeod | | Nationalist | Died | 8 July 1923 | Roderick McLeod | | Nationalist |
| Gippsland South | Thomas Livingston | | Nationalist | Died | 18 August 1922 | Walter West | | Nationalist |
| 26th Legislative Assembly (1920–1921) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Upper Goulburn | Edwin Mackrell | | VFU | Disqualified (failed to nominate on time) | 27 January 1921 | Edwin Mackrell | | VFU |
| 25th Legislative Assembly (1917–1920) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Swan Hill | Percy Stewart | | VFU | Resigned to enter federal politics at the 1919 federal election | 19 November 1919 | Francis Old | | VFU |
| Albert Park | Joseph Hannan | | Labor | Resigned to enter federal politics at the 1919 federal election | 19 November 1919 | Arthur Wallace | | Labor |
| Albert Park | George Elmslie | | Labor | Died | 13 June 1918 | Joseph Hannan | | Labor |
| 24th Legislative Assembly (1914–1917) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Geelong | Robert Purnell | | Nationalist | Disqualified (April by-election declared void) | 17 August 1917 | Robert Purnell | | Nationalist |
| Polwarth | John Johnstone | | Nationalist | Resigned | 13 July 1917 | James McDonald | | Nationalist |
| Benambra | John Leckie | | Nationalist | Resigned | 20 April 1917 | Henry Beardmore | | Nationalist |
| Geelong | William Plain | | Nationalist | Resigned to contest a Senate seat at the 1917 federal election | 20 April 1917 | Robert Purnell | | Nationalist |
| Warrnambool | John Murray | | Commonwealth Liberal | Died | 1 June 1916 | James Deany | | Commonwealth Liberal |
| Port Melbourne | George Sangster | | Labor | Died | 28 April 1915 | Owen Sinclair | | Labor |
| Bendigo East | Alfred Hampson | | Labor | Resigned to enter federal politics at the 1915 Bendigo by-election | 4 February 1915 | Luke Clough | | Labor |
| 23rd Legislative Assembly (1911–1914) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Essendon | William Watt | | Nationalist | Resigned to enter federal politics at the 1914 federal election | 23 July 1914 | Maurice Blackburn | | Labor |
| Gippsland South | Thomas Livingston | | Nationalist | Forfeited seat after joining Peacock Ministry | 30 June 1914 | Thomas Livingston | | Nationalist |
| Korong | Thomas Langdon | | Commonwealth Liberal | Died | 26 June 1914 | Achilles Gray | | Commonwealth Liberal |
| Borung | William Hutchinson | | Commonwealth Liberal | Forfeited seat after joining Watt Ministry | 15 January 1914 | William Hutchinson | | Commonwealth Liberal |
| Essendon | William Watt | | Commonwealth Liberal | Forfeited seat after forming Watt Ministry | 15 January 1914 | William Watt | | Commonwealth Liberal |
| Prahran | Donald Mackinnon | | Commonwealth Liberal | Forfeited seat after joining Watt Ministry | 15 January 1914 | Donald Mackinnon | | Commonwealth Liberal |
| Allandale | Alexander Peacock | | Commonwealth Liberal | Forfeited seat after joining Watt Ministry | 6 January 1914 | Alexander Peacock | | Commonwealth Liberal |
| Castlemaine and Maldon | Harry Lawson | | Commonwealth Liberal | Forfeited seat after joining Watt Ministry | 6 January 1914 | Harry Lawson | | Commonwealth Liberal |
| Warrnambool | John Murray | | Commonwealth Liberal | Forfeited seat after joining Watt Ministry | 6 January 1914 | John Murray | | Commonwealth Liberal |
| Benambra | Albert Craven | | Commonwealth Liberal | Died | 29 December 1913 | John Leckie | | Commonwealth Liberal |
| Albert Park | George Elmslie | | Labor | Forfeited seat after forming Elmslie Ministry | 20 December 1913 | George Elmslie | | Labor |
| Fitzroy | John Billson | | Labor | Forfeited seat after joining Elmslie Ministry | 20 December 1913 | John Billson | | Labor |
| Geelong | William Plain | | Labor | Forfeited seat after joining Elmslie Ministry | 20 December 1913 | William Plain | | Labor |
| Maryborough | Alfred Outtrim | | Labor | Forfeited seat after joining Elmslie Ministry | 20 December 1913 | Alfred Outtrim | | Labor |
| North Melbourne | George Prendergast | | Labor | Forfeited seat after joining Elmslie Ministry | 20 December 1913 | George Prendergast | | Labor |
| Williamstown | John Lemmon | | Labor | Forfeited seat after joining Elmslie Ministry | 20 December 1913 | John Lemmon | | Labor |
| Hawthorn | George Swinburne | | Commonwealth Liberal | Resigned to join the Inter-State Commission | 5 September 1913 | William McPherson | | Commonwealth Liberal |
| Grenville | Charles McGrath | | Labor | Resigned to contest the federal seat of Ballaraat at the 1913 election | 15 May 1913 | John Chatham | | Labor |
| Kara Kara | Peter McBride | | | Resigned to take up appointment as Agent-General for Victoria in London | 14 March 1913 | Jack Pennington | | Commonwealth Liberal |
| Allandale | Alexander Peacock | | Commonwealth Liberal | Forfeited seat after joining Watt Ministry | 6 March 1913 | Alexander Peacock | | Commonwealth Liberal |
| Warrenheip | George Holden | | | Resigned to become chairman of the Melbourne Harbor Trust | 28 February 1913 | Ned Hogan | | Labor |
| East Melbourne | Alfred Farthing | | Commonwealth Liberal | Election declared void | 1 October 1912 | Alfred Farthing | | Commonwealth Liberal |
| Abbotsford | William Beazley | | Labor | Died | 26 July 1912 | Gordon Webber | | Labor |
| 22nd Legislative Assembly (1908–1911) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Waranga | Martin Cussen | | | Died | 25 August 1911 | John Gordon | | |
| Bendigo East | Thomas Glass | | Labor | Died | 16 June 1911 | Alfred Hampson | | Labor |
| Walhalla | Albert Harris | | | Died | 29 July 1910 | Samuel Barnes | | |
| Brunswick | Frank Anstey | | Labor | Resigned to contest the federal seat of Bourke at the 1910 election | 14 March 1910 | James Jewell | | Labor |
| Brighton | Sir Thomas Bent | | Commonwealth Liberal | Died | 8 October 1909 | Oswald Snowball | | Commonwealth Liberal |
| Goulburn Valley | George Graham | | Commonwealth Liberal | Forfeited seat after joining Murray Ministry | 29 January 1909 | George Graham | | Commonwealth Liberal |
| Kara Kara | Peter McBride | | Commonwealth Liberal | Forfeited seat after joining Murray Ministry | 29 January 1909 | Peter McBride | | Commonwealth Liberal |
| Ovens | Alfred Billson | | Commonwealth Liberal | Forfeited seat after joining Murray Ministry | 29 January 1909 | Alfred Billson | | Commonwealth Liberal |
| Rodney | Hugh McKenzie | | Commonwealth Liberal | Forfeited seat after joining Murray Ministry | 29 January 1909 | Hugh McKenzie | | Commonwealth Liberal |
| Warrnambool | John Murray | | Commonwealth Liberal | Forfeited seat after forming Murray Ministry | 29 January 1909 | John Murray | | Commonwealth Liberal |
| 21st Legislative Assembly (1907–1908) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Mornington | Alfred Downward | | | Forfeited seat after joining Bent Ministry | 17 November 1908 | Alfred Downward | | |
| Upper Goulburn | Thomas Hunt | | | Forfeited seat after joining Bent Ministry | 17 November 1908 | Thomas Hunt | | |
| Wangaratta | John Bowser | | | Forfeited seat after joining Bent Ministry | 17 November 1908 | John Bowser | | |
| Carlton | Frederick Bromley | | Labor | Died | 23 October 1908 | Robert Solly | | Labor |
| Richmond | George Bennett | | United Liberal | Died | 2 October 1908 | Ted Cotter | | Labor |
| 20th Legislative Assembly (1904–1907) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Public Officers | David Gaunson | | Labor | resignation | 1 February 1907 | John Carter | | |
| Korong | Thomas Langdon | | United Liberal | Forfeited seat after joining Bent Ministry | 25 January 1907 | Thomas Langdon | | United Liberal |
| East Melbourne | Samuel Gillott | | United Liberal | Resigned due to ill health | 8 January 1907 | Henry Weedon | | |
| Toorak | George Fairbairn | | Ministerialist | Resigned to enter federal politics at the 1906 federal election | 10 October 1906 | Norman Bayles | | Independent Ministerialist |
| Gippsland West | John Mackey | | | Forfeited seat after joining Bent Ministry | 29 August 1906 | John Mackey | | |
| Lowan | William Irvine | | | Resigned to enter federal politics at the 1906 federal election | 20 July 1906 | Robert Stanley | | |
| Barwon | Jonas Levien | | | Died | 15 June 1906 | James Farrer | | |
| Glenelg | Ewen Cameron | | | Died | 11 May 1906 | Hugh Campbell | | |
| 19th Legislative Assembly (1902–1904) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Donald and Swan Hill | John Taverner | | | Resigned to take up appointment as Agent-General for Victoria in London | 9 March 1904 | James Meldrum | | |
| Daylesford | Donald McLeod | | | Forfeited seat after joining Bent Ministry | 27 February 1904 | Donald McLeod | | |
| Melbourne East | Samuel Gillott | | | Forfeited seat after joining Bent Ministry | 27 February 1904 | Samuel Gillott | | |
| East Bourke Boroughs | Frederick Hickford | | | Resigned to contest a House seat at the 1903 federal election | 21 December 1903 | David Methven | | |
| Richmond | William Trenwith | | | Resigned to enter federal politics at the 1903 federal election | 21 December 1903 | George Roberts | | Labor |
| Villiers and Heytesbury | Gratton Wilson | | | Resigned to enter federal politics at the 1903 federal election | 21 December 1903 | John Glasgow | | |
| Mandurang | Maximilian Hirsch | | | Resigned to contest a House seat at the 1903 federal election | 15 December 1903 | William Webb | | |
| Melbourne West | William Maloney | | | Resigned to enter federal politics at the 1903 federal election | 15 December 1903 | Tom Tunnecliffe | | Labor |
| Toorak | Duncan Gillies | | | Died | 9 October 1903 | George Fairbairn | | |
| Bourke West | Samuel Staughton Jr. | | | Died | 6 March 1903 | Andrew Robertson | | |
| Benalla and Yarrawonga | William Hall | | | Died | 25 May 1903 | John Carlisle | | |
| Anglesey | Malcolm McKenzie | | | Resigned | 6 March 1903 | Thomas Hunt | | |
| 18th Legislative Assembly (1900–1902) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Gippsland East | Henry Foster | | | Died | 27 June 1902 | James Cameron | | |
| Anglesey | Malcolm McKenzie | | | Forfeited seat after joining the Irvine Ministry | 18 June 1902 | Malcolm McKenzie | | |
| Brighton | Thomas Bent | | | Forfeited seat after joining the Irvine Ministry | 18 June 1902 | Thomas Bent | | |
| Donald and Swan Hill | John Taverner | | | Forfeited seat after joining the Irvine Ministry | 18 June 1902 | John Taverner | | |
| Evelyn | Ewen Cameron | | | Forfeited seat after joining the Irvine Ministry | 18 June 1902 | Ewen Cameron | | |
| Lowan | William Irvine | | | Forfeited seat after forming the Irvine Ministry | 18 June 1902 | William Irvine | | |
| Normanby | William Shiels | | | Forfeited seat after joining the Irvine Ministry | 18 June 1902 | William Shiels | | |
| Warrnambool | John Murray | | | Forfeited seat after joining the Irvine Ministry | 18 June 1902 | John Murray | | |
| Footscray | Jacob Fotheringham | | | Resigned | 14 June 1902 | Alexander McDonald | | |
| Bourke West | Samuel Staughton Sr. | | | Died | 14 September 1901 | Samuel Staughton Jr. | | |
| Melbourne East | John Anderson | | | Died | 16 July 1901 | John Deegan | | |
| Melbourne | Edward Findley | | Labor | Expelled for seditious libel | 16 July 1901 | James Boyd | | Conservative |
| Bogong | Isaac Isaacs | | | Resigned to enter federal politics at the 1901 federal election | 24 June 1901 | Alfred Billson | | |
| Benalla and Yarrawonga | Thomas Kennedy | | | Resigned to enter federal politics at the 1901 federal election | 17 June 1901 | William Hall | | |
| Fitzroy | Robert Best | | | Resigned to enter federal politics at the 1901 federal election | 17 June 1901 | Patrick O'Connor | | |
| Footscray | Samuel Mauger | | | Resigned to enter federal politics at the 1901 federal election | 17 June 1901 | Jacob Fotheringham | | |
| Gippsland North | Allan McLean | | | Resigned to enter federal politics at the 1901 federal election | 17 June 1901 | Hubert Keogh | | |
| Gunbower | James McColl | | | Resigned to enter federal politics at the 1901 federal election | 17 June 1901 | John Cullen | | |
| Talbot and Avoca | Carty Salmon | | | Resigned to enter federal politics at the 1901 federal election | 17 June 1901 | George Mitchell | | |
| Melbourne East | Samuel Gillott | | | Forfeited seat after joining the Second Turner Ministry | 11 February 1901 | Samuel Gillott | | |
| St Kilda | George Turner | | | Resigned to enter federal politics at the 1901 federal election | 11 February 1901 | William Williams | | |
| St Kilda | George Turner | | | Forfeited seat after forming the Second Turner Ministry | 3 December 1900 | George Turner | | |
| Bogong | Isaac Isaacs | | | Forfeited seat after joining the Second Turner Ministry | 3 December 1900 | Isaac Isaacs | | |
| Clunes and Allandale | Alexander Peacock | | | Forfeited seat after joining the Second Turner Ministry | 27 November 1900 | Alexander Peacock | | |
| Dunolly | Daniel Joseph Duggan | | | Forfeited seat after joining the Second Turner Ministry | 27 November 1900 | Daniel Joseph Duggan | | |
| Geelong | William Gurr | | | Forfeited seat after joining the Second Turner Ministry | 27 November 1900 | William Gurr | | |
| Richmond | William Trenwith | | | Forfeited seat after joining the Second Turner Ministry | 27 November 1900 | William Trenwith | | |
| Rodney | John Morrissey | | | Forfeited seat after joining the Second Turner Ministry | 27 November 1900 | John Morrissey | | |
| Stawell | John Burton | | | Forfeited seat after joining the Second Turner Ministry | 27 November 1900 | John Burton | | |
| 17th Legislative Assembly (1897–1900) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Warrenheip | Edward Murphy | | | Died | 11 May 1900 | George Holden | | |
| Grenville | George Russell | | | Resigned | 11 April 1900 | James Sadler | | |
| Talbot and Avoca | Carty Salmon | | | Forfeited seat after joining the McLean Ministry | 9 January 1900 | Carty Salmon | | |
| Castlemaine | James McCay | | | Forfeited seat after joining the McLean Ministry | 20 December 1899 | James McCay | | |
| Melbourne North | William Watt | | | Forfeited seat after joining the McLean Ministry | 20 December 1899 | William Watt | | |
| Gunbower | James McColl | | | Forfeited seat after joining the McLean Ministry | 13 December 1899 | James McColl | | |
| Gippsland North | Allan McLean | | | Forfeited seat after forming the McLean Ministry | 13 December 1899 | Allan McLean | | |
| Lowan | William Irvine | | | Forfeited seat after joining the McLean Ministry | 13 December 1899 | William Irvine | | |
| Maryborough | Alfred Outtrim | | | Forfeited seat after joining the McLean Ministry | 13 December 1899 | Alfred Outtrim | | |
| Normanby | William Shiels | | | Forfeited seat after joining the McLean Ministry | 13 December 1899 | William Shiels | | |
| Numurkah and Nathalia | George Graham | | | Forfeited seat after joining the McLean Ministry | 13 December 1899 | George Graham | | |
| Footscray | John Hancock | | | Died | 12 December 1899 | Samuel Mauger | | |
| Grenville | Michael Stapleton | | | Died | 1 December 1899 | David Kerr | | |
| Melbourne East | Ephraim Zox | | | Died | 7 November 1899 | Samuel Gillott | | |
| Windermere | William Anderson | | | Died | 3 June 1898 | John Spiers | | |
| 16th Legislative Assembly (1894–1897) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Kara Kara | Andrew Anderson | | | Died | 11 May 1897 | Peter McBride | | |
| Melbourne South | Joseph Winter | | | Died | 26 May 1896 | John Tucker | | |
| Villiers and Heytesbury | Thomas Scott | | | Resigned | 26 May 1896 | John McArthur | | |
| Castlemaine | James Patterson | | | Died | 19 November 1895 | James McCay | | |
| Mornington | Alfred Downward | | | Election declared void | 26 January 1895 | Alfred Downward | | |
| Ballarat West | William Smith | | | Died | 5 November 1894 | Joseph Kirton | | |
| Carlton South | William Ievers | | | Died | 5 November 1894 | John Barrett | | |
| Bogong | Isaac Isaacs | | | Forfeited seat after joining the First Turner Ministry | 9 October 1894 | Isaac Isaacs | | |
| Clunes and Allandale | Alexander Peacock | | | Forfeited seat after joining the First Turner Ministry | 3 October 1894 | Alexander Peacock | | |
| Donald and Swan Hill | John Taverner | | | Forfeited seat after joining the First Turner Ministry | 3 October 1894 | John Taverner | | |
| Eaglehawk | Henry Williams | | | Forfeited seat after joining the First Turner Ministry | 3 October 1894 | Henry Williams | | |
| Fitzroy | Robert Best | | | Forfeited seat after joining the First Turner Ministry | 3 October 1894 | Robert Best | | |
| Gippsland East | Henry Foster | | | Forfeited seat after joining the First Turner Ministry | 3 October 1894 | Henry Foster | | |
| Kilmore, Dalhousie and Lancefield | John Duffy | | | Forfeited seat after joining the First Turner Ministry | 3 October 1894 | John Duffy | | |
| St Kilda | George Turner | | | Forfeited seat after forming the First Turner Ministry | 3 October 1894 | George Turner | | |
| 15th Legislative Assembly (1892–1894) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Portland | Henry Wrixon | | | Resigned | 24 July 1894 | Donald McLeod | | |
| Grenville | David Davies | | | Died | 14 July 1894 | George Russell | | |
| Eastern Suburbs | Duncan Gillies | | | Resigned to take up appointment as Agent-General for Victoria in London | 25 January 1894 | Frank Madden | | |
| Windermere | Matthew Butterley | | | Died | 25 January 1894 | William Anderson | | |
| Talbot and Avoca | Robert Bowman | | | Died | 29 December 1893 | Carty Salmon | | |
| Benalla and Yarrawonga | John Templeton | | | Election declared void | 20 November 1893 | Thomas Kennedy | | |
| Benalla and Yarrawonga | James Campbell | | | Died | 9 October 1893 | John Templeton | | |
| Lowan | Richard Baker | | | Forfeited seat after joining the Patterson Ministry | 9 October 1893 | Richard Baker | | |
| Sandhurst | Robert Burrowes | | | Died | 6 October 1893 | Daniel Lazarus | | |
| Creswick | Richard Richardson | | | Forfeited seat after joining the Patterson Ministry | 28 August 1893 | Richard Richardson | | |
| Mandurang | John Highett | | | Died | 10 July 1893 | Richard O'Neill | | |
| Bogong | Isaac Isaacs | | | Resigned | 6 June 1893 | Isaac Isaacs | | |
| Melbourne North | David Wyllie | | | Died | 5 June 1893 | Sylvanus Reynolds | | |
| Benalla and Yarrawonga | James Campbell | | | Forfeited seat after joining the Patterson Ministry | 31 January 1893 | James Campbell | | |
| Bogong | Isaac Isaacs | | | Forfeited seat after joining the Patterson Ministry | 31 January 1893 | Isaac Isaacs | | |
| Castlemaine | James Patterson | | | Forfeited seat after forming the Patterson Ministry | 31 January 1893 | James Patterson | | |
| Gunbower | James McColl | | | Forfeited seat after joining the Patterson Ministry | 31 January 1893 | James McColl | | |
| Kara Kara | John Dow | | | Resigned due to insolvency | 31 January 1893 | John Dow | | |
| Maldon | John McIntyre | | | Forfeited seat after joining the Patterson Ministry | 31 January 1893 | John McIntyre | | |
| Melbourne | Godfrey Carter | | | Forfeited seat after joining the Patterson Ministry | 31 January 1893 | Godfrey Carter | | |
| Port Fairy | Bryan O'Loghlen | | | Forfeited seat after joining the Patterson Ministry | 31 January 1893 | Bryan O'Loghlen | | |
| Rodney | William Webb | | | Forfeited seat after joining the Patterson Ministry | 31 January 1893 | William Webb | | |
| Dundas | Samuel Samuel | | | Died | 18 August 1892 | John Thomson | | |
| East Bourke Boroughs | Graham Berry | | | Forfeited seat after joining the Shiels Ministry | 3 May 1892 | Graham Berry | | |
| 14th Legislative Assembly (1889–1892) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Benalla and Yarrawonga | John Brock | | | Resigned | 2 April 1892 | Charles Kerville | | |
| Geelong | James Munro | | | Resigned to take up appointment as Agent-General for Victoria in London | 2 April 1892 | John Hopkins | | |
| Clunes and Allandale | Alexander Peacock | | | Forfeited seat after joining the Shiels Ministry | 3 March 1892 | Alexander Peacock | | |
| Bourke East | William Wilkinson | | | Died | 27 August 1891 | Robert Harper | | |
| St Kilda | George Turner | | | Forfeited seat after joining the Munro Ministry | 7 May 1891 | George Turner | | |
| Collingwood | George Langridge | | | Died | 17 April 1891 | John Hancock | | |
| Collingwood | George Langridge | | | Forfeited seat after joining the Munro Ministry | 20 November 1890 | George Langridge | | |
| Daylesford | James Wheeler | | | Forfeited seat after joining the Munro Ministry | 20 November 1890 | James Wheeler | | |
| Geelong | James Munro | | | Forfeited seat after forming the Munro Ministry | 20 November 1890 | James Munro | | |
| Gippsland North | Allan McLean | | | Forfeited seat after joining the Munro Ministry | 20 November 1890 | Allan McLean | | |
| Kilmore, Dalhousie and Lancefield | John Duffy | | | Forfeited seat after joining the Munro Ministry | 20 November 1890 | John Duffy | | |
| Maryborough | Alfred Outtrim | | | Forfeited seat after joining the Munro Ministry | 20 November 1890 | Alfred Outtrim | | |
| Normanby | William Shiels | | | Forfeited seat after joining the Munro Ministry | 20 November 1890 | William Shiels | | |
| Numurkah and Nathalia | George Graham | | | Forfeited seat after joining the Munro Ministry | 20 November 1890 | George Graham | | |
| Kara Kara | John Dow | | | Forfeited seat after joining the Gillies Ministry | 17 September 1890 | John Dow | | |
| Villiers and Heytesbury | William Anderson | | | Forfeited seat after joining the Gillies Ministry | 17 September 1890 | William Anderson | | |
| Dunolly | James Cheetham | | | Died | 22 July 1890 | William Tatchell | | |
| Talbot and Avoca | James Stewart | | | Died | 4 December 1889 | Robert Bowman | | |
| Ballarat East | James Russell | | | Died | 8 November 1889 | John Dunn | | |
| 13th Legislative Assembly (1886–1889) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Belfast | John Madden | | | Died | 2 February 1888 | Bryan O'Loghlen | | |
| Williamstown | Alfred Clark | | | Resigned | 2 November 1887 | James Mirams | | |
| Dalhousie | George Sands | | | Died | 25 March 1887 | John Duffy | | |
| Benambra | Peter Wallace | | | Died | 15 June 1886 | Peter Wright | | |
| 12th Legislative Assembly (1883–1886) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Ovens | George Kerferd | | | Resigned to take up appointment on the Supreme Court of Victoria | 15 January 1886 | Ferguson Tuthill | | |
| Maryborough and Talbot | Robert Bowman | | | Resigned | 22 December 1885 | Alfred Outtrim | | |
| Melbourne West | James Orkney | | | Resigned | 30 October 1885 | Godfrey Carter | | |
| Avoca | James Grant | | | Died | 24 April 1885 | George Bourchier | | |
| Mandurang | Hugh McColl | | | Died | 24 April 1885 | John Highett | | |
| Moira | Henry Bolton | | | Resigned | 2 May 1884 | George Graham | | |
| Warrnambool | James Francis | | | Died | 15 February 1884 | John Murray | | |
| Emerald Hill | Robert MacGregor | | | Died | 9 October 1883 | David Gaunson | | |
| Sandhurst | Robert Clark | | | Died | 15 May 1883 | Angus Mackay | | |
| Barwon | Jonas Levien | | | Forfeited seat after joining the Second Service Ministry | 24 March 1883 | Jonas Levien | | |
| Bourke West | Alfred Deakin | | | Forfeited seat after joining the Second Service Ministry | 24 March 1883 | Alfred Deakin | | |
| Castlemaine | James Service | | | Forfeited seat after forming the Second Service Ministry | 24 March 1883 | James Service | | |
| Collingwood | George Langridge | | | Forfeited seat after joining the Second Service Ministry | 24 March 1883 | George Langridge | | |
| Fitzroy | Albert Tucker | | | Forfeited seat after joining the Second Service Ministry | 24 March 1883 | Albert Tucker | | |
| Geelong | Graham Berry | | | Forfeited seat after joining the Second Service Ministry | 24 March 1883 | Graham Berry | | |
| Ovens | George Kerferd | | | Forfeited seat after joining the Second Service Ministry | 24 March 1883 | George Kerferd | | |
| Rodney | Duncan Gillies | | | Forfeited seat after joining the Second Service Ministry | 24 March 1883 | Duncan Gillies | | |
| Melbourne North | James Rose | | | Resigned | 21 March 1883 | James Rose | | |
| 11th Legislative Assembly (1880–1883) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Bourke East | Robert Ramsay | | | Died | 13 June 1882 | Robert Harper | | |
| Geelong | Charles Kernot | | | Died | 18 April 1882 | Joseph Connor | | |
| Boroondara | Robert Smith | | | Resigned to take up appointment as Agent-General for Victoria in London | 23 February 1882 | William Walker | | |
| Geelong | Robert Johnstone | | | Died | 8 December 1881 | Robert Johnstone | | |
| Fitzroy | William Vale | | | Resigned | 6 December 1881 | Cuthbert Blackett | | |
| Sandhurst | Robert Burrowes | | | Forfeited seat after joining the O'Loghlen Ministry | 6 September 1881 | Robert Burrowes | | |
| Wimmera | Walter Madden | | | Forfeited seat after joining the O'Loghlen Ministry | 6 September 1881 | Walter Madden | | |
| Polwarth and South Grenville | William O'Hea | | | Died | 5 August 1881 | William Robertson | | |
| Ararat | David Gaunson | | | Forfeited seat after joining the O'Loghlen Ministry | 26 July 1881 | David Gaunson | | |
| Avoca | James Grant | | | Forfeited seat after joining the O'Loghlen Ministry | 26 July 1881 | James Grant | | |
| Bourke West | Bryan O'Loghlen | | | Forfeited seat after forming the O'Loghlen Ministry | 26 July 1881 | Bryan O'Loghlen | | |
| Brighton | Thomas Bent | | | Forfeited seat after joining the O'Loghlen Ministry | 26 July 1881 | Thomas Bent | | |
| Delatite | James Graves | | | Forfeited seat after joining the O'Loghlen Ministry | 26 July 1881 | James Graves | | |
| Kyneton Boroughs | Charles Young | | | Forfeited seat after joining the O'Loghlen Ministry | 26 July 1881 | Charles Young | | |
| Moira | Henry Bolton | | | Forfeited seat after joining the O'Loghlen Ministry | 26 July 1881 | Henry Bolton | | |
| Melbourne North | Joseph Storey | | | Died | 8 April 1881 | James Munro | | |
| Maldon | James Service | | | Resigned | 18 March 1881 | John McIntyre | | |
| Melbourne East | Alexander Smith | | | Died | 8 February 1881 | Frederick Walsh | | |
| Ballarat West | William Smith | | | Forfeited seat after joining the Third Berry Ministry | 18 August 1880 | William Smith | | |
| Castlemaine | James Patterson | | | Forfeited seat after joining the Third Berry Ministry | 18 August 1880 | James Patterson | | |
| Collingwood | George Langridge | | | Forfeited seat after joining the Third Berry Ministry | 18 August 1880 | George Langridge | | |
| Creswick | Richard Richardson | | | Forfeited seat after joining the Third Berry Ministry | 18 August 1880 | Richard Richardson | | |
| Fitzroy | William Vale | | | Forfeited seat after joining the Third Berry Ministry | 18 August 1880 | William Vale | | |
| Geelong | Graham Berry | | | Forfeited seat after forming the Third Berry Ministry | 18 August 1880 | Graham Berry | | |
| Mandurang | Henry Williams | | | Forfeited seat after joining the Third Berry Ministry | 18 August 1880 | Henry Williams | | |
| Williamstown | Alfred Clark | | | Forfeited seat after joining the Third Berry Ministry | 18 August 1880 | Alfred Clark | | |
| 10th Legislative Assembly (1880) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Bourke East | Robert Ramsay | | | Forfeited seat after joining the First Service Ministry | 19 March 1880 | Robert Ramsay | | |
| Brighton | Thomas Bent | | | Forfeited seat after joining the First Service Ministry | 19 March 1880 | Thomas Bent | | |
| Dalhousie | John Duffy | | | Forfeited seat after joining the First Service Ministry | 19 March 1880 | John Duffy | | |
| Maldon | James Service | | | Forfeited seat after forming the First Service Ministry | 19 March 1880 | James Service | | |
| Ovens | George Kerferd | | | Forfeited seat after joining the First Service Ministry | 19 March 1880 | George Kerferd | | |
| Rodney | Duncan Gillies | | | Forfeited seat after joining the First Service Ministry | 19 March 1880 | Duncan Gillies | | |
| Sandhurst | Robert Clark | | | Forfeited seat after joining the First Service Ministry | 19 March 1880 | Robert Clark | | |
| Sandridge | John Madden | | | Forfeited seat after joining the First Service Ministry | 19 March 1880 | John Madden | | |
| 9th Legislative Assembly (1877–1880) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Villiers and Heytesbury | Jeremiah Dwyer | | | Declared Insolvent | 16 December 1879 | Joseph Jones | | |
| Carlton | James Munro | | | Resigned | 2 December 1879 | James Munro | | |
| Bourke West | Alfred Deakin | | | Resigned | 22 August 1879 | Robert Harper | | |
| Melbourne East | Ephraim Zox | | | Resigned | 1 July 1879 | Ephraim Zox | | |
| Fitzroy | Robert MacGregor | | | Resigned | 25 June 1879 | Cuthbert Blackett | | |
| Footscray | Mark Last King | | | Died | 4 March 1879 | William Clark | | |
| Bourke West | John Smith | | | Died | 18 February 1879 | Alfred Deakin | | |
| Dundas | John MacPherson | | | Resigned | 29 July 1878 | John Serjeant | | |
| Gippsland South | George Macartney | | | Died | 19 June 1878 | Francis Mason | | |
| Castlemaine | James Farrell | | | Resigned | 7 June 1878 | Charles Pearson | | |
| Warrnambool | James McCulloch | | | Resigned | 21 May 1878 | James Francis | | |
| Melbourne West | Bryan O'Loghlen | | | Forfeited seat after joining the Second Berry Ministry | 11 April 1878 | Bryan O'Loghlen | | |
| Melbourne West | Charles MacMahon | | | Resigned | 4 February 1878 | Bryan O'Loghlen | | |
| Boroondara | George Smith | | | Died | 24 December 1877 | Robert Smith | | |
| Rodney | Duncan Gillies | | | Election declared void | 2 November 1877 | Duncan Gillies | | |
| Gippsland South | George Macartney | | | Election declared void | 24 August 1877 | George Macartney | | |
| Sandhurst | W. G. Blackham | | | Election declared void | 25 July 1877 | Angus Mackay | | |
| Avoca | James Grant | | | Forfeited seat after joining the Second Berry Ministry | 7 June 1877 | James Grant | | |
| Ballarat West | William Smith | | | Forfeited seat after joining the Second Berry Ministry | 7 June 1877 | William Smith | | |
| Castlemaine | James Patterson | | | Forfeited seat after joining the Second Berry Ministry | 7 June 1877 | James Patterson | | |
| Geelong | Graham Berry | | | Forfeited seat after forming the Second Berry Ministry | 7 June 1877 | Graham Berry | | |
| Grant | Peter Lalor | | | Forfeited seat after joining the Second Berry Ministry | 7 June 1877 | Peter Lalor | | |
| Ripon and Hampden | Francis Longmore | | | Forfeited seat after joining the Second Berry Ministry | 7 June 1877 | Francis Longmore | | |
| Stawell | John Woods | | | Forfeited seat after joining the Second Berry Ministry | 7 June 1877 | John Woods | | |
| 8th Legislative Assembly (1874–1877) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Gippsland North | James McKean | | | Expelled | 17 August 1876 | Charles Duffy | | |
| Sandridge | David Thomas | | | Died | 27 July 1876 | John Madden | | |
| Geelong East | John Richardson | | | Died | 29 March 1876 | Charles Kernot | | |
| Collingwood | James Sullivan | | | Died | 22 February 1876 | James Mirams | | |
| East Bourke Boroughs | George Higinbotham | | | Resigned | 10 February 1876 | William Cook | | |
| Villiers and Heytesbury | Michael O'Grady | | | Died | 27 January 1876 | Joseph Jones | | |
| Bourke East | Robert Ramsay | | | Forfeited seat after joining the Fourth McCulloch Ministry | 8 November 1875 | Robert Ramsay | | |
| Bourke West | John Madden | | | Forfeited seat after joining the Fourth McCulloch Ministry | 8 November 1875 | Mark King | | |
| Ballarat West | Joseph Jones | | | Forfeited seat after joining the Fourth McCulloch Ministry | 6 November 1875 | George Fincham | | |
| Warrnambool | James McCulloch | | | Forfeited seat after forming the Fourth McCulloch Ministry | 6 November 1875 | James McCulloch | | |
| Ararat | William McLellan | | | Forfeited seat after joining the Fourth McCulloch Ministry | 2 November 1875 | William McLellan | | |
| Dundas | John MacPherson | | | Forfeited seat after joining the Fourth McCulloch Ministry | 2 November 1875 | John MacPherson | | |
| Maryborough | Duncan Gillies | | | Forfeited seat after joining the Fourth McCulloch Ministry | 2 November 1875 | Duncan Gillies | | |
| Ovens | George Kerferd | | | Forfeited seat after joining the Fourth McCulloch Ministry | 2 November 1875 | George Kerferd | | |
| Avoca | James Grant | | | Forfeited seat after joining the First Berry Ministry | 20 August 1875 | James Grant | | |
| Ballarat West | William Smith | | | Forfeited seat after joining the First Berry Ministry | 20 August 1875 | William Smith | | |
| Castlemaine | James Patterson | | | Forfeited seat after joining the First Berry Ministry | 20 August 1875 | James Patterson | | |
| Crowlands | John Woods | | | Forfeited seat after joining the First Berry Ministry | 20 August 1875 | John Woods | | |
| Geelong West | Graham Berry | | | Forfeited seat after forming the First Berry Ministry | 20 August 1875 | Graham Berry | | |
| Grant South | Peter Lalor | | | Forfeited seat after joining the First Berry Ministry | 20 August 1875 | Peter Lalor | | |
| Melbourne North | James Munro | | | Forfeited seat after joining the First Berry Ministry | 20 August 1875 | James Munro | | |
| Ripon and Hampden | Francis Longmore | | | Forfeited seat after joining the First Berry Ministry | 20 August 1875 | Francis Longmore | | |
| Ararat | Michael Carroll | | | Forfeited seat | 19 March 1875 | David Gaunson | | |
| Gippsland North | Frederick Smyth | | | Resigned | 19 March 1875 | James McKean | | |
| Richmond | James Francis | | | Resigned | 8 December 1874 | Joseph Bosisto | | |
| Bourke East | Robert Ramsay | | | Forfeited seat after joining the Kerferd Ministry | 18 August 1874 | Robert Ramsay | | |
| Ballarat East | Townsend McDermott | | | Forfeited seat after joining the Kerferd Ministry | 12 August 1874 | Townsend McDermott | | |
| Maldon | James Service | | | Forfeited seat after joining the Kerferd Ministry | 12 August 1874 | James Service | | |
| Grenville | Mark Pope | | | Died | 31 July 1874 | Richard Lock | | |
| Collingwood | John Everard | | | Resigned | 29 July 1874 | George Langridge | | |
| St Kilda | James Stephen | | | Resigned to take up appointment on the Supreme Court of Victoria | 15 May 1874 | Edward Dixon | | |
| 7th Legislative Assembly (1871–1874) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Ovens | George Kerferd | | | Forfeited seat after joining the Francis Ministry | 11 May 1874 | George Kerferd | | |
| East Bourke Boroughs | William Champ | | | Resigned | 26 May 1873 | George Higinbotham | | |
| Portland | Howard Spensley | | | Resigned | 20 May 1873 | Thomas Must | | |
| Grenville | Thomas Russell | | | Resigned | 24 February 1873 | John Montgomery | | |
| St Kilda | Thomas Fellows | | | Resigned to take up appointment on the Supreme Court of Victoria | 13 January 1873 | Robert Murray Smith | | |
| Murray | William Witt | | | Resigned | 8 July 1872 | John Orr | | |
| Mandurang | James Casey | | | Forfeited seat after joining the Francis Ministry | 26 June 1872 | James Casey | | |
| Maryborough | Duncan Gillies | | | Forfeited seat after joining the Francis Ministry | 26 June 1872 | Duncan Gillies | | |
| Melbourne East | Edward Cohen | | | Forfeited seat after joining the Francis Ministry | 26 June 1872 | Edward Cohen | | |
| Melbourne West | Edward Langton | | | Forfeited seat after joining the Francis Ministry | 26 June 1872 | Edward Langton | | |
| Ovens | George Kerferd | | | Forfeited seat after joining the Francis Ministry | 26 June 1872 | George Kerferd | | |
| Richmond | James Francis | | | Forfeited seat after forming the Francis Ministry | 26 June 1872 | James Francis | | |
| St Kilda | James Stephen | | | Forfeited seat after joining the Francis Ministry | 26 June 1872 | James Stephen | | |
| Sandhurst | Angus Mackay | | | Forfeited seat after joining the Francis Ministry | 26 June 1872 | Angus Mackay | | |
| Mornington | James McCulloch | | | Resigned | 26 March 1872 | James Purves | | |
| Collingwood | William Vale | | | Forfeited seat after joining the Duffy Ministry | 9 December 1871 | William Vale | | |
| Ballarat East | Robert Walsh | | | Forfeited seat after joining the Duffy Ministry | 18 July 1871 | Robert Walsh | | |
| Ararat | William McLellan | | | Forfeited seat after joining the Duffy Ministry | 30 June 1871 | William McLellan | | |
| Avoca | James Grant | | | Forfeited seat after joining the Duffy Ministry | 30 June 1871 | James Grant | | |
| Dalhousie | Charles Duffy | | | Forfeited seat after forming the Duffy Ministry | 30 June 1871 | Charles Duffy | | |
| Geelong West | Graham Berry | | | Forfeited seat after joining the Duffy Ministry | 30 June 1871 | Graham Berry | | |
| Portland | Howard Spensley | | | Forfeited seat after joining the Duffy Ministry | 30 June 1871 | Howard Spensley | | |
| Ripon and Hampden | Francis Longmore | | | Forfeited seat after joining the Duffy Ministry | 30 June 1871 | Francis Longmore | | |
| Villiers and Heytesbury | Michael O'Grady | | | Forfeited seat after joining the Duffy Ministry | 30 June 1871 | Michael O'Grady | | |
| 6th Legislative Assembly (1868–1871) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Creswick | William Frazer | | | Died | 30 December 1870 | James Stewart | | |
| Castlemaine | William Baillie | | | Resigned | 5 December 1870 | James Patterson | | |
| Bourke East | Matthew McCaw | | | Resigned | 24 September 1870 | Robert Ramsay | | |
| St Kilda | Butler Aspinall | | | Resigned | 13 September 1870 | James Stephen | | |
| Avoca | James Grant | | | Resigned | 25 July 1870 | Peter Finn | | |
| Villiers and Heytesbury | Morgan McDonnell | | | Resigned | 25 July 1870 | Michael O'Grady | | |
| Geelong West | James Carr | | | Resigned | 6 May 1870 | Robert Johnstone | | |
| Ballarat West | John James | | | Resigned | 30 April 1870 | Archibald Michie | | |
| Ararat | William Wilson | | | Forfeited seat after joining the Third McCulloch Ministry | 26 April 1870 | William Wilson | | |
| Dundas | Alexander MacPherson | | | Forfeited seat after joining the Third McCulloch Ministry | 26 April 1870 | Alexander MacPherson | | |
| Belfast | Henry Wrixon | | | Forfeited seat after joining the Third McCulloch Ministry | 25 April 1870 | Henry Wrixon | | |
| Collingwood | William Bates | | | Forfeited seat after joining the Third McCulloch Ministry | 25 April 1870 | William Bates | | |
| Mornington | James McCulloch | | | Forfeited seat after forming the Third McCulloch Ministry | 25 April 1870 | James McCulloch | | |
| Richmond | James Francis | | | Forfeited seat after joining the Third McCulloch Ministry | 25 April 1870 | James Francis | | |
| Sandhurst | Angus Mackay | | | Forfeited seat after joining the Third McCulloch Ministry | 25 April 1870 | Angus Mackay | | |
| Maryborough | Robert Bowman | | | Resigned | 29 March 1870 | Duncan Gillies | | |
| Geelong West | Graham Berry | | | Forfeited seat after joining the MacPherson Ministry | 7 February 1870 | Graham Berry | | |
| St Kilda | Butler Aspinall | | | Forfeited seat after joining the MacPherson Ministry | 1 February 1870 | Butler Aspinall | | |
| Ararat | William McLellan | | | Forfeited seat after joining the MacPherson Ministry | 29 January 1870 | William McLellan | | |
| Crowlands | Robert Byrne | | | Forfeited seat after joining the MacPherson Ministry | 9 October 1869 | George Rolfe | | |
| Ripon and Hampden | Francis Longmore | | | Forfeited seat after joining the MacPherson Ministry | 9 October 1869 | Francis Longmore | | |
| Villiers and Heytesbury | Morgan McDonnell | | | Forfeited seat after joining the MacPherson Ministry | 9 October 1869 | Morgan McDonnell | | |
| Dundas | John MacPherson | | | Forfeited seat after forming the MacPherson Ministry | 8 October 1869 | John MacPherson | | |
| Bourke West | John Smith | | | Forfeited seat after joining the MacPherson Ministry | 7 October 1869 | John Smith | | |
| Maryborough | James McKean | | | Forfeited seat after joining the MacPherson Ministry | 7 October 1869 | James McKean | | |
| Collingwood | Isaac Reeves | | | Forfeited seat after joining the MacPherson Ministry | 5 October 1869 | William Vale | | |
| Melbourne East | Edward Cohen | | | Forfeited seat after joining the MacPherson Ministry | 5 October 1869 | Edward Cohen | | |
| Ararat | William Wilson | | | Forfeited seat after joining the Second McCulloch Ministry | 14 September 1869 | William Wilson | | |
| Ballarat West | William Vale | | | Resigned | 23 May 1869 | John James | | |
| Ballarat West | Charles Jones | | | Expelled for Corruption | 10 May 1869 | Charles Jones | | |
| Portland | James Butters | | | Expelled for Bribery | 5 May 1869 | James Butters | | |
| Ballarat West | Charles Jones | | | Expelled for Bribery | 27 March 1869 | Charles Jones | | |
| Gipps Land South | Thomas McCombie | | | Resigned | 19 March 1869 | George Macartney | | |
| Castlemaine | Samuel Bindon | | | Resigned | 30 October 1868 | Richard Kitto | | |
| Geelong West | Nicholas Foott | | | Died | 16 October 1868 | Graham Berry | | |
| Bourke East | James Balfour | | | Resigned | 29 August 1868 | William Lobb | | |
| Ovens | George Smith | | | Forfeited seat after joining the Second McCulloch Ministry | 1 August 1868 | George Smith | | |
| Ballarat West | Charles Jones | | | Forfeited seat after joining the Second McCulloch Ministry | 30 July 1868 | Charles Jones | | |
| Ballarat West | William Vale | | | Forfeited seat after joining the Second McCulloch Ministry | 30 July 1868 | William Vale | | |
| Bourke South | George Smith | | | Forfeited seat after joining the Second McCulloch Ministry | 30 July 1868 | George Smith | | |
| Mornington | James McCulloch | | | Forfeited seat after forming the Second McCulloch Ministry | 30 July 1868 | James McCulloch | | |
| Avoca | James Grant | | | Forfeited seat after joining the Second McCulloch Ministry | 25 July 1868 | James Grant | | |
| Mandurang | James Casey | | | Forfeited seat after joining the Second McCulloch Ministry | 25 July 1868 | James Casey | | |
| Mandurang | James Sullivan | | | Forfeited seat after joining the Second McCulloch Ministry | 25 July 1868 | James Sullivan | | |
| Emerald Hill | George Verdon | | | Resigned to take up appointment as Agent-General for Victoria in London | 17 June 1868 | John Whiteman | | |
| Ovens | George Kerferd | | | Forfeited seat after joining the Sladen Ministry | 27 May 1868 | George Kerferd | | |
| Villiers and Heytesbury | William Bayles | | | Forfeited seat after joining the Sladen Ministry | 27 May 1868 | William Bayles | | |
| Villiers and Heytesbury | Morgan McDonnell | | | Forfeited seat after joining the Sladen Ministry | 27 May 1868 | Morgan McDonnell | | |
| Ballarat West | Duncan Gillies | | | Forfeited seat after joining the Sladen Ministry | 25 May 1868 | Charles Jones | | |
| Bourke South | Michael O'Grady | | | Forfeited seat after joining the Sladen Ministry | 25 May 1868 | John Crews | | |
| Melbourne West | Edward Langton | | | Forfeited seat after joining the Sladen Ministry | 19 May 1868 | Edward Langton | | |
| St Kilda | Thomas Fellows | | | Forfeited seat after joining the Sladen Ministry | 19 May 1868 | Thomas Fellows | | |
| 5th Legislative Assembly (1866–1868) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Grant South | William McCann | | | Imprisoned for Forgery | 13 September 1867 | William Stutt | | |
| Dalhousie | George Sands | | | Resigned | 12 August 1867 | Charles Duffy | | |
| Maldon | John Ramsay | | | Died | 14 June 1867 | William Williams | | |
| Mandurang | James Sullivan | | | Forfeited seat after joining the First McCulloch Ministry | 25 March 1867 | James Sullivan | | |
| Richmond | Archibald Wardrop | | | Resigned | 25 September 1866 | Ambrose Kyte | | |
| Ballarat West | William Vale | | | Forfeited seat after joining the First McCulloch Ministry | 6 August 1866 | William Vale | | |
| Castlemaine | Samuel Bindon | | | Forfeited seat after joining the First McCulloch Ministry | 1 August 1866 | Samuel Bindon | | |
| Rodney | John MacGregor | | | Forfeited seat after joining the First McCulloch Ministry | 1 August 1866 | John MacGregor | | |
| 4th Legislative Assembly (1864–1866) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Ballarat West | William Vale | | | Forfeited seat after joining the First McCulloch Ministry | 11 September 1865 | William Vale | | |
| 3rd Legislative Assembly (1861–1864) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| East Bourke Boroughs | Richard Heales | | | Died | 9 July 1864 | Edward Cope | | |
| Villiers and Heytesbury | Richard Ireland | | | Resigned | 19 May 1864 | Samuel MacGregor | | |
| Gipps Land North | George Mackay | | | Resigned | 11 April 1864 | John Everard | | |
| Ballarat West | William Smith | | | Resigned | 4 February 1864 | Robert Lewis | | |
| Mandurang | John Owens | | | Resigned | 12 August 1863 | James Casey | | |
| Polwarth and South Grenville | William Nixon | | | Resigned | 27 July 1863 | Archibald Michie | | |
| Avoca | James Grant | | | Forfeited seat after joining the First McCulloch Ministry | 18 July 1863 | James Grant | | |
| Mandurang | James Sullivan | | | Forfeited seat after joining the First McCulloch Ministry | 15 July 1863 | James Sullivan | | |
| Mornington | James McCulloch | | | Forfeited seat after forming the First McCulloch Ministry | 15 July 1863 | James McCulloch | | |
| East Bourke Boroughs | Richard Heales | | | Forfeited seat after joining the First McCulloch Ministry | 11 July 1863 | Richard Heales | | |
| Richmond | James Francis | | | Forfeited seat after joining the First McCulloch Ministry | 10 July 1863 | James Francis | | |
| Brighton | George Higinbotham | | | Forfeited seat after joining the First McCulloch Ministry | 9 July 1863 | George Higinbotham | | |
| Williamstown | George Verdon | | | Forfeited seat after joining the First McCulloch Ministry | 9 July 1863 | George Verdon | | |
| Bourke South | Louis Smith | | | Resigned | 3 June 1863 | Louis Smith | | |
| Evelyn | William Jones | | | Resigned | 5 May 1863 | John Thompson | | |
| Rodney | Wilson Gray | | | Resigned | 3 November 1862 | John MacGregor | | |
| Gipps Land South | George Hedley | | | Resigned | 24 October 1862 | John Johnson | | |
| Sandhurst | William Denova | | | Resigned | 30 August 1862 | Robert Strickland | | |
| Ripon and Hampden | James Service | | | Resigned | 29 August 1862 | George Morton | | |
| Castlemaine | Vincent Pyke | | | Resigned | 10 July 1862 | George Smyth | | |
| Ararat | Daniel Flint | | | Resigned | 10 April 1862 | Tharp Girdlestone | | |
| Brighton | William Brodribb | | | Resigned | 7 April 1862 | George Higinbotham | | |
| Grenville | Robert Gillespie | | | Resigned | 17 March 1862 | Mark Pope | | |
| Mornington | Henry Chapman | | | Resigned | 24 February 1862 | James McCulloch | | |
| Maryborough | George Evans | | | Forfeited seat after joining the Third O'Shanassy Ministry | 13 January 1862 | George Evans | | |
| Portland | William Haines | | | Forfeited seat after joining the Third O'Shanassy Ministry | 10 December 1861 | William Haines | | |
| Villiers and Heytesbury | Charles Duffy | | | Forfeited seat after joining the Third O'Shanassy Ministry | 3 December 1861 | Charles Duffy | | |
| Villiers and Heytesbury | Richard Ireland | | | Forfeited seat after joining the Third O'Shanassy Ministry | 3 December 1861 | Richard Ireland | | |
| Warrnambool | Thomas Manifold | | | Resigned | 3 December 1861 | John Wood | | |
| St Kilda | James Johnston | | | Forfeited seat after joining the Third O'Shanassy Ministry | 28 November 1861 | James Johnston | | |
| Emerald Hill | Robert Anderson | | | Forfeited seat after joining the Third O'Shanassy Ministry | 27 November 1861 | Robert Anderson | | |
| Kilmore | John O'Shanassy | | | Forfeited seat after forming the Third O'Shanassy Ministry | 27 November 1861 | John O'Shanassy | | |
| Melbourne North | Patrick Costello | | | Expelled for Electoral Fraud | 18 November 1861 | John Sinclair | | |
| Castlemaine | James Chapman | | | Disqualified | November 1861 | Alexander Smith | | |
| Gipps Land North | John Everard | | | Declared Insolvent | 24 October 1861 | George Mackay | | |
| Maryborough | Richard Ireland | | | Resigned | 21 October 1861 | George Evans | | |
| 2nd Legislative Assembly (1859–1861) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| Melbourne East | Alexander Hunter | | | Resigned | 2 July 1861 | Graham Berry | | |
| Mandurang | James Sullivan | | | Forfeited seat after joining the Heales Ministry | 26 June 1861 | James Sullivan | | |
| Mandurang | George Brodie | | | Resigned | 10 June 1861 | James Sullivan | | |
| Crowlands | John Houston | | | Forfeited seat after joining the Heales Ministry | 10 June 1861 | John Houston | | |
| Brighton | Charles Ebden | | | Resigned | 20 May 1861 | George Higinbotham | | |
| Castlemaine | John Macadam | | | Forfeited seat after joining the Heales Ministry | 13 May 1861 | Alexander Smith | | |
| Wimmera | Robert Firebrace | | | Resigned | 18 May 1861 | Samuel Wilson | | |
| Villiers and Heytesbury | Alexander Russell | | | Resigned | 1 April 1861 | William Rutledge | | |
| Melbourne West | Thomas Loader | | | Forfeited seat after joining the Heales Ministry | 27 March 1861 | Thomas Loader | | |
| Avoca | James Grant | | | Forfeited seat after joining the Heales Ministry | 11 March 1861 | James Grant | | |
| Mandurang | George Brodie | | | Forfeited seat after joining the Heales Ministry | 8 March 1861 | George Brodie | | |
| Warrnambool | George Horne | | | Resigned | 4 March 1861 | Thomas Manifold | | |
| Ballarat West | John Bailey | | | Resigned | 23 February 1861 | Duncan Gillies | | |
| Bourke South | Hibbert Newton | | | Forfeited seat after joining the Heales Ministry | 13 December 1860 | Hibbert Newton | | |
| Ballarat West | John Bailey | | | Forfeited seat after joining the Heales Ministry | 12 December 1860 | John Bailey | | |
| Ballarat East | John Humffray | | | Forfeited seat after joining the Heales Ministry | 11 December 1860 | John Humffray | | |
| Maryborough | Richard Ireland | | | Forfeited seat after joining the Heales Ministry | 11 December 1860 | Nathaniel Levi | | |
| East Bourke Boroughs | Richard Heales | | | Forfeited seat after forming the Heales Ministry | 10 December 1860 | Richard Heales | | |
| Geelong West | John Brooke | | | Forfeited seat after joining the Heales Ministry | 10 December 1860 | John Brooke | | |
| Melbourne West | Thomas Loader | | | Forfeited seat after joining the Heales Ministry | 10 December 1860 | Thomas Loader | | |
| St Kilda | James Johnston | | | Forfeited seat after joining the Heales Ministry | 10 December 1860 | James Johnston | | |
| Emerald Hill | Robert Anderson | | | Forfeited seat after joining the Heales Ministry | 8 December 1860 | Robert Anderson | | |
| Williamstown | George Verdon | | | Forfeited seat after joining the Heales Ministry | 8 December 1860 | George Verdon | | |
| Gipps Land South | Angus McMillan | | | Resigned | 23 November 1860 | George Hedley | | |
| Castlemaine | Vincent Pyke | | | Resigned | 17 October 1860 | Vincent Pyke | | |
| Castlemaine | Butler Aspinall | | | Resigned | 9 October 1860 | James Chapman | | |
| Geelong West | James Harrison | | | Resigned | 9 October 1860 | Nicholas Foott | | |
| Geelong East | Augustus Greeves | | | Forfeited seat after joining the Nicholson Ministry | 6 October 1860 | Augustus Greeves | | |
| Portland | Norman McLeod | | | Resigned | September 1860 | William Haines | | |
| Evelyn | William Bell | | | Resigned | 27 March 1860 | William Jones | | |
| Ovens | Alexander Keefer | | | Resigned | 26 March 1860 | John Donald | | |
| Maldon | George Harker | | | Resigned | 13 March 1860 | James Martley | | |
| Bourke West | Patrick Phelan | | | Election declared void | 13 February 1860 | John Riddell | | |
| Geelong East | James Cowie | | | Resigned | 7 February 1860 | Augustus Greeves | | |
| Rodney | John Everard | | | Resigned | 11 January 1860 | Wilson Gray | | |
| Evelyn | John King | | | Resigned | 31 December 1859 | William Bell | | |
| Richmond | James Francis | | | Forfeited seat after joining the Nicholson Ministry | 9 December 1859 | James Francis | | |
| Ovens | John Wood | | | Forfeited seat after joining the Nicholson Ministry | 14 November 1859 | John Wood | | |
| Ripon and Hampden | James Service | | | Forfeited seat after joining the Nicholson Ministry | 14 November 1859 | James Service | | |
| Evelyn | John King | | | Forfeited seat after joining the Nicholson Ministry | 11 November 1859 | John King | | |
| Ballarat West | John Bailey | | | Forfeited seat after joining the Nicholson Ministry | 9 November 1859 | John Bailey | | |
| Castlemaine | Vincent Pyke | | | Forfeited seat after joining the Nicholson Ministry | 9 November 1859 | Vincent Pyke | | |
| Melbourne East | James McCulloch | | | Forfeited seat after joining the Nicholson Ministry | 8 November 1859 | James McCulloch | | |
| Sandridge | William Nicholson | | | Forfeited seat after forming the Nicholson Ministry | 8 November 1859 | William Nicholson | | |
| 1st Legislative Assembly (1856–1859) |||||||||
| By-election | Incumbent | Party | Party | Reason | Date | Winner | Party | Party |
| The Murray | William Forlonge | | | Resigned | 1 January 1859 | William Nicholson | | |
| South Grant | William Haines | | | Resigned | 1 January 1859 | John Bell | | |
| South Melbourne | Andrew Clarke | | | Resigned | 1 October 1858 | Robert Anderson | | |
| St Kilda | Thomas Fellows | | | Resigned | 1 May 1858 | John Crews | | |
| Dundas and Follett | Charles Griffith | | | Resigned | 1 April 1858 | William Mollison | | |
| Geelong | Charles Read | | | Resigned | 1 April 1858 | James Harrison | | |
| Geelong | Alexander Fyfe | | | Resigned | 1 February 1858 | George Board | | |
| St Kilda | Frederick Sargood | | | Resigned | 5 January 1858 | Henry Chapman | | |
| The Murray | John Goodman | | | Resigned | 1 January 1858 | William Forlonge | | |
| Rodney | John Baragwanath | | | Resigned | 1 January 1858 | John Everard | | |
| Geelong | Charles Sladen | | | Resigned | 1 December 1857 | Alexander Thomson | | |
| Gippsland | John King | | | Resigned | 1 November 1857 | John Johnson | | |
| Castlemaine Boroughs | Alexander Palmer | | | Resigned | 1 August 1857 | Richard Davies Ireland | | |
| West Bourke | Robert McDougall | | | Resigned | 1 August 1857 | Joseph Wilkie | | |
| Colac | Andrew Rutherford | | | Resigned | 1 July 1857 | Theodore Hancock | | |
| Portland | Hugh Childers | | | Resigned | 1 July 1857 | John Findlay | | |
| South Bourke | Charles Pasley | | | Resigned | 1 July 1857 | Sidney Ricardo | | |
| Ovens | Daniel Cameron | | | Resigned | 1 April 1857 | John Wood | | |
| Brighton | Jonathan Binns Were | | | Resigned | 1 March 1857 | Charles Ebden | | |
| Castlemaine Boroughs | Vincent Pyke | | | Resigned | 1 March 1857 | Robert Sitwell | | |
| East Bourke | Augustus Greeves | | | Resigned | 1 March 1857 | Richard Heales | | |
| Melbourne | William Stawell | | | Resigned | 1 March 1857 | James Service | | |
| Melbourne | John O'Shanassy | | | Resigned; having been elected to a seat for both the Melbourne and Kilmore districts, he decided to represented the latter. | 1 January 1857 | Henry Langlands | | |