# AADACL2

Arylacetamide Deacetylase Like 2, also known as AADACL2, is a protein which in humans is encoded by the AADACL2 gene.

## Gene

The AADACL2 gene consists of 27413 nucleotides with an mRNA of approximately 5060 base pairs. There are five exons found in the AADACL2 gene. AADACL2 is found to function in catalytic activity, hydrolase activity, and found to enable carboxylic ester hydrolase activity.

## mRNA

The chromosomal band location of AADACL2 is 3q25.1 and is located on the plus strand of the DNA.

## Protein

AADACL2 in humans is 401 amino acids long. with a predicted molecular weight of 46 kDa. The isoelectric point of AADACL2 is 7.2. The AADACL2 protein contains two domains, Abhydrolase_3, that spans nearly the entire protein. Along with an intermediate domain which is Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole. Both of which are found to be conserved amongst orthologs.

### Localization

Human AADACL2 is predicted to be localized in the endoplasmic reticulum.

## Expression

AADACL2 is tissue specific. AADACL2 is expressed at a very high level in skin tissues. It is found to express at low levels in placenta, esophagus, small intestine and colon. It is also found in circular RNA induction during fetal development at higher levels at the intestines at 10 weeks and 20 weeks.

## Tertiary structure

The protein AADACL2 was found to be composed of both alpha helices along with beta barrels.

## Evolution

The protein encoded by AADACL2 evolves slower than the fibrinogen alpha protein but faster than the protein cytochrome c.

### Paralogs

Paralogs of AADACL2 include: AADAC (Arylacetamide Deacetylase), NCEH1 (Neutral Cholesterol Ester Hydrolase 1), AADACL3 (Arylacetamide Deacetylase Like 3), AADACL4 (Arylacetamide Deacetylase Like 4), and AFMID (Arylformamidase).

### Orthologs

Arylacetamide Deacetylase like 2 is found only in mammals. AADACL2 is not found in fish, birds, reptiles, and amphibians. Arylacetamide Deacetylase like 2 is found in the following mammals: primates, flying lemurs, bats, rabbits & hares, rodents, insectivores, afrotheria, carnivores, even-toed ungulates, and odd-toed ungulates. Arylacetamide Deacetylase like 2 was approximately found to have first appeared in afrotheria 99 million years ago.
| AADACL2 | Genus and Species | Common Name | Taxonomic Group | Median Date of Divergence (MYA) | Protein Accession # | Protein Sequence Length (aa) | Sequence Identity to Human Protein (%) | Sequence Similarity to Human Protein (%) |
| Primates | Homo sapiens | Human | Primates | 0 | NP_997248.2 | 401 | 100.0 | 100.0 |
| | Macaca mulatta | Rhesus monkey | Primates | 28.8 | XP_001106757.2 | 401 | 98 | 99.3 |
| | Rhinopithecus roxellana | Golden snub-nosed monkey | Primates | 28.8 | XP_010370407.2 | 401 | 97.3 | 99.0 |
| | Sapajus apella | Tufted capuchin | Primates | 43 | XP_032118900.1 | 401 | 94 | 96.3 |
| | Aotus nancymaae | Nancy Ma's monkey | Primates | 43 | XP_012292432.2 | 401 | 94.5 | 96.5 |
| | Microcebus murinus | Gray mouse lemur | Primates | 74 | XP_012646764.1 | 402 | 72.1 | 81.8 |
| Flying Lemurs | Cynocephalus volans | Philippine flying lemur | Dermoptera | 74 | XP_062934601.1 | 401 | 82.5 | 89.0 |
| Bats | Rhinolophus ferrumequinum | Greater Horseshoe Bat | Bats | 94 | XP_032991506.1 | 401 | 81.0 | 87.8 |
| | Pteropus alecto | Black flying fox | Chiroptera | 94 | XP_006926324.1 | 343 | 50.5 | 63.6 |
| Rabbits & Hares | Ochotona princeps | American Pika | Lagomorpha | 87 | XP_004598151.2 | 401 | 80.5 | 88.3 |
| Rodents | Octodon degus | Degu | Rodentia | 87 | XP_004643988.1 | 401 | 80.0 | 89.0 |
| | Mus musculus | Mouse | Rodentia | 87 | NP_001121563 | 401 | 79.1 | 89.5 |
| | Marmota monax | Woodchuck | Rodentia | 87 | XP_046287168.1 | 401 | 65.1 | 78.1 |
| Insectivores | Condylura cristata | Star-nosed Mole | Eulipotyphla | 94 | XP_004682396.1 | 401 | 78.8 | 86.3 |
| Afrotheria | Trichechus manatus latirostris | Florida Manatee | Sirenia | 99 | XP_004379276.3 | 402 | 78.4 | 88.1 |
| | Elephas maximus indicus | Indian Elephant | Proboscideans | 99 | XP_049723395.1 | 402 | 78.1 | 86.8 |
| Carnivores | Lontra canadensis | Northern American River Otter | Carnivora | 94 | XP_032708555.1 | 401 | 75.1 | 84.0 |
| | Phoca vitulina | Harbor Seal | Carnivora | 94 | XP_032265082.1 | 400 | 73.8 | 82.0 |
| | Ursus maritimus | Polar Bear | Carnivora | 94 | XP_008705296.2 | 401 | 73.6 | 83.3 |
| | Mustela putorius furo | Domestic Ferret | Carnivora | 94 | XP_004755622.1 | 401 | 73.6 | 82.5 |
| | Canis lupus dingo | Dingo | Carnivora | 94 | XP_025291970.3 | 403 | 73.4 | 81.9 |
| | Ailuropoda melanoleuca | Giant Panda | Carnivora | 94 | XP_002916395.1 | 397 | 72.1 | 81.0 |
| | Odobenus rosmarus divergens | Pacific Walrus | Carnivora | 94 | XP_004405057.1 | 400 | 70.3 | 80.5 |
| Even-Toed Ungulates | Hippopotamus amphibius kiboko | Common Hippopotamus | Artiodactyls | 94 | XP_057593784.1 | 416 | 70.0 | 79.4 |
| | Camelus dromedarius | Arabian Camel | Artiodactyls | 94 | XP_010985013.1 | 401 | 63.8 | 78.1 |
| Odd-Toed Ungulates | Equus caballus | Horse | Perissodactyla | 94 | XP_014587028.1 | 587 | 49.6 | 56.6 |

## Clinical significance

Allergic contact dermatitis exposed to a nickel allergy, indicates that there is lower presence in the AADACL2 RNA when exposed to a nickel allergy versus the non-allergic control. Arylacetamide deacetylase-like 2 was found to be in the top fifty skin enriched genes, and was predicted to contain a signal peptide and function as a secreted protein.