# List of Billboard number-one dance singles of 2000

These are the Billboard Hot Dance/Disco Club Play and Maxi-Singles Sales number-one hits of 2000.
| Date | Club Play Song | Artist | Maxi-Singles Sales | Artist |
| January 1 | "17 Again" | Eurythmics | "Sexual (Li Da Di)" | Amber |
| January 8 | "Fly Away (Bye Bye)" | Eyes Cream | "Sexual (Li Da Di)" | Amber |
| January 15 | "Supersonic" | Jamiroquai | "Sexual (Li Da Di)" | Amber |
| January 22 | "Above the Clouds" | Amber | "Sexual (Li Da Di)" | Amber |
| January 29 | "That Sound" | Michael Moog | "Sexual (Li Da Di)" | Amber |
| February 5 | "Take a Picture" | Filter | "Sexual (Li Da Di)" | Amber |
| February 12 | "Temperamental" | Everything but the Girl | "Sexual (Li Da Di)" | Amber |
| February 19 | "I Learned from the Best" | Whitney Houston | "I Learned from the Best" | Whitney Houston |
| February 26 | "I Learned from the Best" | Whitney Houston | "Maria Maria" | Santana |
| March 4 | "I Learned from the Best" | Whitney Houston | "Maria Maria" | Santana |
| March 11 | "Stop Playing with My Mind" | Barbara Tucker featuring Darryl D'Bonneau | "I Learned from the Best" | Whitney Houston |
| March 18 | "Planet Love" | Taylor Dayne | "Maria Maria" | Santana |
| March 25 | "Chase" | Giorgio Moroder vs. Jam & Spoon | "Say My Name" | Destiny's Child |
| April 1 | "American Pie" | Madonna | "Say My Name" | Destiny's Child |
| April 8 | "Think It Over" | Jennifer Holliday | "Say My Name" | Destiny's Child |
| April 15 | "King of My Castle" | Wamdue Project | "Say My Name" | Destiny's Child |
| April 22 | "Missing You" | Kim English | "Say My Name" | Destiny's Child |
| April 29 | "It Feels So Good" | Sonique | "Say My Name" | Destiny's Child |
| May 6 | "Be with You" | Enrique Iglesias | "Say My Name" | Destiny's Child |
| May 13 | "If It Don't Fit" | Abigail | "Say My Name" | Destiny's Child |
| May 20 | "I'm in Love" | Veronica | "Say My Name" | Destiny's Child |
| May 27 | "I Will Love Again" | Lara Fabian | "Say My Name" | Destiny's Child |
| June 3 | "Feelin' So Good" | Jennifer Lopez featuring Big Pun and Fat Joe | "Desert Rose" (Victor Calderone Remix) | Sting Featuring Cheb Mami |
| June 10 | "When I Get Close to You" | Jocelyn Enriquez | "Desert Rose" (Victor Calderone Remix) | Sting Featuring Cheb Mami |
| June 17 | "Show Me" | Angel Clivillés | "Desert Rose" (Victor Calderone Remix) | Sting Featuring Cheb Mami |
| June 24 | "Flash" | Green Velvet | "Desert Rose" (Victor Calderone Remix) | Sting Featuring Cheb Mami |
| July 1 | "I'm Not in Love" | Olive | "Desert Rose" (Victor Calderone Remix) | Sting Featuring Cheb Mami |
| July 8 | "Don't You Want My Love" | Rosabel featuring Debbie Jacobs-Rock | "Desert Rose" (Victor Calderone Remix) | Sting Featuring Cheb Mami |
| July 15 | "Don't You Want My Love" | Rosabel featuring Debbie Jacobs-Rock | "Desert Rose" (Victor Calderone Remix) | Sting Featuring Cheb Mami |
| July 22 | "Don't Call Me Baby" | Madison Avenue | "Desert Rose" (Victor Calderone Remix) | Sting Featuring Cheb Mami |
| July 29 | "Bingo Bango" | Basement Jaxx | "Desert Rose" (Victor Calderone Remix) | Sting Featuring Cheb Mami |
| August 5 | "Bingo Bango" | Basement Jaxx | "Jumpin', Jumpin'" | Destiny's Child |
| August 12 | "I Never Knew" | Deborah Cox | "Let's Get Married" | Jagged Edge |
| August 19 | "Spanish Guitar" | Toni Braxton | "Let's Get Married" | Jagged Edge |
| August 26 | "Desire" | Ultra Naté | "Let's Get Married" | Jagged Edge |
| September 2 | "Desire" | Ultra Naté | "Let's Get Married" | Jagged Edge |
| September 9 | "Stronger" | Kristine W | "Music" † | Madonna |
| September 16 | "Music" † | Madonna | "Music" † | Madonna |
| September 23 | "Music" † | Madonna | "Music" † | Madonna |
| September 30 | "Music" † | Madonna | "Music" † | Madonna |
| October 7 | "Music" † | Madonna | "Music" † | Madonna |
| October 14 | "Music" † | Madonna | "Music" † | Madonna |
| October 21 | "Your Child" | Mary J. Blige | "Music" † | Madonna |
| October 28 | "Don't Want Another Man" | Dynamix presents Tina Ann | "Music" † | Madonna |
| November 4 | "Lovin' Is Really My Game" | Ann Nesby | "Music" † | Madonna |
| November 11 | "I Turn to You" | Melanie C | "Music" † | Madonna |
| November 18 | "Pasilda" | Afro Medusa | "Music" † | Madonna |
| November 25 | "Pasilda" | Afro Medusa | "Most Girls" | P!nk |
| December 2 | "Love One Another" | Amber | "Most Girls" | P!nk |
| December 9 | "Dreamin'" | Loleatta Holloway | "Most Girls" | P!nk |
| December 16 | "Independent Women Part I" | Destiny's Child | "Most Girls" | P!nk |
| December 23 | "Livin' for Love" | Natalie Cole | "Independent Women Part I" | Destiny's Child |
| December 30 | "One More Time" | Daft Punk | "Independent Women Part I" | Destiny's Child |