# List of largest National Wildlife Refuges

Here is a list of the largest National Wildlife Refuges in the United States. It includes all that are larger than 50,000 acres (200 km$^2$), but excludes those in U.S. territories (also officially in the system). Acreage/Area includes water as well as land areas. Statistics are as of 30 September 2007. The eleven largest National Wildlife Refuges are all in the state of Alaska.
| Name | State(s) | Acreage | Area: km² |
| Arctic National Wildlife Refuge | Alaska | 19,286,482.30 | 78,049.62 |
| Yukon Delta National Wildlife Refuge | Alaska | 19,162,416.50 | 77,547.55 |
| Yukon Flats National Wildlife Refuge | Alaska | 8,634,511.57 | 34,942.63 |
| Togiak National Wildlife Refuge | Alaska | 4,102,537.38 | 16,602.38 |
| Innoko National Wildlife Refuge | Alaska | 3,850,481.05 | 15,582.34 |
| Alaska Peninsula National Wildlife Refuge | Alaska | 3,563,489.83 | 14,420.93 |
| Koyukuk National Wildlife Refuge | Alaska | 3,550,160.50 | 14,366.99 |
| Alaska Maritime National Wildlife Refuge | Alaska | 3,417,756.91 | 13,831.17 |
| Selawik National Wildlife Refuge | Alaska | 2,150,161.99 | 8,701.40 |
| Kodiak National Wildlife Refuge | Alaska | 1,990,418.11 | 8,054.94 |
| Kenai National Wildlife Refuge | Alaska | 1,912,425.40 | 7,739.31 |
| Desert National Wildlife Refuge | Nevada | 1,615,313.49 | 6,536.94 |
| Nowitna National Wildlife Refuge | Alaska | 1,560,000.00 | 6,313.10 |
| Kanuti National Wildlife Refuge | Alaska | 1,430,160.17 | 5,787.65 |
| Becharof National Wildlife Refuge | Alaska | 1,200,059.57 | 4,856.47 |
| Charles M. Russell National Wildlife Refuge | Montana | 915,294.22 | 3,704.06 |
| Cabeza Prieta National Wildlife Refuge | Arizona | 860,041.32 | 3,480.46 |
| Tetlin National Wildlife Refuge | Alaska | 700,058.54 | 2,833.04 |
| Kofa National Wildlife Refuge | Arizona | 666,561.26 | 2,697.48 |
| Sheldon National Wildlife Refuge | Nevada / Oregon | 573,503.63 | 2,320.89 |
| Okefenokee National Wildlife Refuge | Georgia / Florida | 401,908.38 | 1,626.47 |
| Izembek National Wildlife Refuge | Alaska | 311,075.78 | 1,258.88 |
| Hart Mountain National Antelope Refuge | Oregon | 270,003.58 | 1,092.67 |
| Hawaiian Islands National Wildlife Refuge | Hawaii | 254,418.10 | 1,029.59 |
| Sevilleta National Wildlife Refuge | New Mexico | 229,673.57 | 929.46 |
| Key West National Wildlife Refuge | Florida | 208,308.17 | 842.99 |
| Upper Mississippi River National Wildlife and Fish Refuge | Wisconsin / Iowa / Minnesota / Illinois | 208,143.53 | 842.33 |
| Hanford Reach National Monument | Washington | 195,719.00 | 702.05 |
| Malheur National Wildlife Refuge | Oregon | 187,126.94 | 757.28 |
| White River National Wildlife Refuge | Arkansas | 158,976.07 | 643.35 |
| Alligator River National Wildlife Refuge | North Carolina | 153,017.15 | 619.24 |
| Arthur R. Marshall Loxahatchee National Wildlife Refuge | Florida | 143,953.77 | 582.56 |
| Merritt Island National Wildlife Refuge | Florida | 139,189.40 | 563.28 |
| Sabine National Wildlife Refuge | Louisiana | 125,790.08 | 509.05 |
| Great White Heron National Wildlife Refuge | Florida | 117,720.63 | 476.40 |
| Buenos Aires National Wildlife Refuge | Arizona | 117,107.16 | 473.92 |
| Aransas National Wildlife Refuge | Texas | 114,657.05 | 464.00 |
| Great Dismal Swamp National Wildlife Refuge | Virginia / North Carolina | 111,202.81 | 450.02 |
| Pocosin Lakes National Wildlife Refuge | North Carolina | 110,106.64 | 445.59 |
| Seney National Wildlife Refuge | Michigan | 95,244.81 | 385.44 |
| Lower Rio Grande Valley National Wildlife Refuge | Texas | 90,441.09 | 366.00 |
| Grasslands Wildlife Management Area | California | 89,012.27 | 360.22 |
| Laguna Atascosa National Wildlife Refuge | Texas | 88,388.69 | 357.70 |
| Stillwater National Wildlife Refuge | Nevada | 87,271.26 | 353.17 |
| National Key Deer Refuge | Florida | 84,824.28 | 343.27 |
| Baca National Wildlife Refuge | Colorado | 78,696.65 | 318.47 |
| Bear River Migratory Bird Refuge | Utah | 73,996.41 | 299.45 |
| Tensas River National Wildlife Refuge | Louisiana | 73,169.36 | 296.11 |
| Valentine National Wildlife Refuge | Nebraska | 73,098.09 | 295.82 |
| Saint Marks National Wildlife Refuge | Florida | 69,196.75 | 280.03 |
| Cape Romain National Wildlife Refuge | South Carolina | 66,287.18 | 268.25 |
| Cache River National Wildlife Refuge | Arkansas | 66,164.82 | 267.76 |
| Red Rock Lakes National Wildlife Refuge | Montana | 65,810.25 | 266.32 |
| Felsenthal National Wildlife Refuge | Arkansas | 64,902.14 | 262.65 |
| Agassiz National Wildlife Refuge | Minnesota | 61,500.93 | 248.89 |
| J. Clark Salyer National Wildlife Refuge | North Dakota | 59,376.40 | 240.29 |
| Wichita Mountains Wildlife Refuge | Oklahoma | 59,019.60 | 238.84 |
| McFaddin National Wildlife Refuge | Texas | 58,861.43 | 238.20 |
| San Andres National Wildlife Refuge | New Mexico | 57,215.48 | 231.54 |
| Bosque del Apache National Wildlife Refuge | New Mexico | 57,191.10 | 231.44 |
| UL Bend National Wildlife Refuge | Montana | 56,049.56 | 226.82 |
| Tennessee National Wildlife Refuge | Tennessee | 51,359.46 | 207.84 |
| Lower Suwannee National Wildlife Refuge | Florida | 51,030.32 | 206.51 |
| Big Oaks National Wildlife Refuge | Indiana | 51,000.00 | 206.39 |
| Lower Klamath National Wildlife Refuge | California / Oregon | 50,912.68 | 206.04 |
| Mattamuskeet National Wildlife Refuge | North Carolina | 50,173.93 | 203.05 |