# CXorf58

Human chromosome X open reading frame 58 (CXorf58) is a protein coding gene encoding the CXorf58 protein.

## Gene

CXorf58 contains 9 exons. CXorf58 is 31,578 nucleotides long. The longest exon in CXorf58 is exon 1, containing 324 nucleotides and the shortest exon is exon 4, containing 111 nucleotides. CXorf58 is highly expressed in sperm cells, particularly at the lower point of the head and midpiece of spermatids.

## Isoforms

There are two isoforms for CXorf58 protein. Isoform 1 for CXorf58 is 332 amino acids long and isoform 2 is 330 amino acids long. Isoform 2 uses an alternate in-frame splice site in the 3' coding region, resulting in a shorter protein.

## Protein

The molecular weight of CXorf58 isoform 1 protein is 38.9kDa. It is rich in basic amino acids and abundant in the amino acids methionine and arginine. CXorf58 contains 3 asparagine-linked glycosylation sites and 5 phosphorylation sites.

## Gene level regulation

CXorf58 contains a tissue-specific expression pattern. It is found in high abundance in the testis.

## Protein level regulation

CXorf58 is localized in the cytoplasm of cells. CXorf58 contains 10 net phosphorylation sites. There is one glycosylation site for CXorf58 at amino acid 320.

## Homology

Orthologs of CXorf58 appear in an array of taxonomic groups.
| Species | Common Name | NCBI Accession Number | Sequence Length (amino acids) | Date of Divergence (MYA) | Sequence Identity (%) | Sequence Similarity (%) |
| Homo sapiens | Human | XP_054182731.1 | 332 | - | - | - |
| Gorilla gorilla gorilla | Western lowland gorilla | XP_004063966.2 | 332 | 8.6 | 99% | 99% |
| Sciurus carolinensis | Eastern gray squirrel | XP_047392635.1 | 370 | 87 | 62% | 69% |
| Mus Musculus | House mouse | NP_001349810.1 | 368 | 87 | 56% | 65% |
| Equus quagga | Plains zebra | XP_046529678.1 | 374 | 94 | 64% | 71% |
| Panthera uncia | Snow leopard | XP_049499554.1 | 380 | 94 | 64% | 66% |
| Cervus canadensis | Elk | XP_043314584.1 | 372 | 94 | 63% | 69% |
| Bos taurus | Cattle | XP_024843724.1 | 370 | 94 | 63% | 68% |
| Struthio camelus australis | South African ostrich | XP_009663695.1 | 342 | 319 | 49% | 54% |
| Dromaius novaehollandiae | Emu | XP_025959174.1 | 334 | 319 | 48% | 53% |
| Nothoprocta perdicaria | Chilean tinamou | XP_025894453.1 | 332 | 319 | 47% | 51% |
| Chrysemys picta bellii | Painted turtle | XP_065435515.1 | 408 | 319 | 48% | 53% |
| Chelonia mydas | Green sea turtle | XP_037757767.1 | 353 | 319 | 48% | 54% |
| Danio rerio | Zebrafish | XP_021332053.1 | 253 | 429 | 44% | 65% |
| Asterias rubens | Common starfish | XP_033637843.1 | 404 | 619 | 44% | 53% |
| Saccoglossus kowalevskii | Acorn worm | XP_002736551.1 | 390 | 619 | 44% | 53% |
| Ruditapes philippinarum | Manila clam | XP_060606373.1 | 446 | 686 | 45% | 42% |
| Lineus longissimus | Bootlace worm | XP_064639061.1 | 393 | 686 | 45% | 52% |
| Liolophura japonica | Chiton liolophura japonica | XP_064615141.1 | 435 | 686 | 45% | 45% |
| Patella vulgata | Common limpet | XP_050389200.1 | 434 | 686 | 41% | 45% |

## Interacting proteins

Five proteins have interactions with CXorf58: GDE1, H2BC9, HOXA1, TGFBR2, and DCAF5.
| Protein | Protein Full Name | Function |
| GDE1 | Glycerophosphodiester Phosphodiesterase 1 | Enables glycerophosphodiester phosphodiesterase activity, glycerophosphoinositol glycerophosphodiesterase activity, and lysophospholipase activity. |
| H2BC9 | H2B Clustered Histone 9 | Encodes a replication-dependent histone that is a member of the histone H2B family. |
| HOXA1 | Homeobox A1 | Encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. |
| TGFBR2 | Transforming Growth Factor Beta Receptor 2 | Forms a heterodimeric complex with TGF-beta receptor type-1, and binds TGF-beta. |
| DCAF5 | DDB1 and CUL4 Associated Factor 5 | Involved in protein ubiquitination. |

## Clinical significance

Mutations that occur on CXorf58 include deletions of base pairs, missense mutations, nonsense mutations, and frameshift mutations. Many of which are coding sequence variants.