# Klamath Lake AFA

Klamath Lake AFA, also called Klamath Lake Blue Green Algae and Klamath AFA (Aphanizomenon flos-aquae MDT14a), is a strain of Aphanizomenon flos-aquae. Small amounts of this cyanobacteria can be found in bodies of water worldwide, but it is notable for growing prolifically in Upper Klamath Lake, Oregon. Klamath AFA is a blue-green algae that has been harvested wild from Upper Klamath Lake since the 1980s and used as a dietary supplement.
Genome sequencing distinguished and named this isolate as Aphanizomenon flos-aquae MDT14a, distinct from other varieties of Aphanizomenon flos-aquae. Aphanizomenon flos-aquae was historically treated as a single homogeneous species, but genetic technology reveals significant diversity within the group, with at least 18 separate genomes identified and potentially over 100 awaiting classification.

## Taxonomy and distinctions between toxic and non-toxic strains

The classification of Aphanizomenon flos-aquae has undergone significant taxonomic revision in light of recent genetic studies. Genome sequencing has revealed substantial genetic variability, leading to the identification of distinct strains found around the world, such as strains like AFA FACHB-1287, AFA FACHB-1265, AFA NRERC-008, isolates like AFA KM1D3_PB, AFA UKL13-PB, and others.
These advancements have clarified a longstanding misconception regarding toxin production in AFA samples from Upper Klamath Lake. Earlier studies attributed the presence of cyanotoxins such as microcystin and cylindrospermopsin directly to endogenous production by AFA. However, research employing genetic sequencing and species-specific analysis has revealed that these toxins were not capable of being produced by Klamath AFA itself but were instead the result of cross-contamination from co-occurring toxin-producing cyanobacteria inhabiting the same environment.

## Habitat and distribution

Upper Klamath Lake (also called Klamath Lake) in the Cascade Range of south-central Oregon hosts a viable and harvestable population of Aphanizomenon flos-aquae MDT14a. While this subspecies has been detected in other water bodies, these populations are either too sparse or are mixed with other aquatic species, making harvesting impractical.
Klamath Lake's high levels of dissolved minerals, large surface area, shallow depths, and other nutritional and environmental factors create suitable conditions for the proliferation of Aphanizomenon flos-aquae MDT14a. Some of these factors are the lake's high 4,100 feet (1,259 m) elevation, eutrophic nutrient levels, high alkalinity (8.5 pH or higher), a large surface area of 96 square miles with an average depth of 8 feet, and large number of sunny days (130 to 300)throughout the year.

## Controversies

Klamath AFA has been proven to be incapable of endogenously producing microcystins or other toxins. However, Aphanizomenon flos-aquae species typically cohabit with other cyanobacteria, most commonly with the Microcystis species.
Cross-contamination of products containing Klamath AFA have occurred in the past. From 2018 to 2020, the FDA did three product recalls, all by the same original harvesting company. Each recall found higher levels of microcystin than suggested by the WHO and EFA provisional guidelines, which is less than 1 microgram per gram. These investigations led to Class 2 voluntary recalls of the affected products. The products were all linked to several batches of AFA harvested by the company between 2015 and 2017. From these recalls, the FDA started working with harvesting companies to outline new industry practices and testing procedures for harvesting AFA. These now include:
* At the time of harvest, examining AFA at the harvesting site for contaminating cyanobacteria.
* At the time of harvest, testing the water and AFA for microcystins.
* After harvest, testing the AFA slurry for microcystins.
* Before selling AFA products, testing each lot or batch for microcystins.
* Using more than one test method to confirm results.
* Using a test method that can detect multiple variant forms of microcystins.
* Using certified testing laboratories and validated methods.
* Providing a certificate of analysis to purchasers for each lot or batch sold.
Since these guidelines were developed, no product recalls have occurred. As of 2024, the FDA states that wild-harvested blue-green algae is safe to eat.