# C14orf80

Uncharacterized protein C14orf80 is a protein which in humans is encoded by the chromosome 14 open reading frame 80, C14orf80, gene.

## Gene
### Location

C14orf80 is located on chromosome 14 (14q32.33) starting at 105,489,855bp and ending at 105,499,248bp. C14orf80 is 9,393 base pairs long and contains 11 exons that can be alternatively spliced to form different mRNA variants.

### Variants

Transcription of C14orf80 can produce 19 mRNA splice variants. Only six of these nineteen variants are predicted to not encode for a protein. Of the mRNA variants that have been found experimentally, the longest is 1,719 base pairs and produces a protein with 426 amino acids.

### Expression

C14orf80 has been determined to be expressed in 77 types of tissues and 100 developmental stages. It has also been determined to have a higher level of expression in a few cases of pancreatic and prostate cancer cells compared to normal tissue.

## Homology
### Paralogs

There are no paralogs of C14orf80.

### Orthologs

Using the BLAST program from NCBI, the orthologs of C14orf80 were found to range from primates to invertebrates. Below is a table that contains a variety of these orthologs.
| Species | Common name | Accession number | Date of divergence | Sequence length (AA) | Sequence similarity |
| Homo sapiens | Human | NP_001128347 | 0 mya | 426 | 100% |
| Chlorocebus sabaeus | Green monkey | XP_007986247 | 29 mya | 424 | 94% |
| Ictidomys tridecemlineatus | 13-lined ground squirrel | XP_005334680 | 92.3 mya | 426 | 80% |
| Bos taurus | Cow | XP_003585026 | 94.2 mya | 419 | 78% |
| Rattus norvegicus | Brown rat | XP_002726827 | 92.3 mya | 420 | 76% |
| Zonotrichia albicollis | White-throated sparrow | XP_005493655 | 296 mya | 454 | 53% |
| Pelodiscus sinensis | Chinese softshell turtle | XP_006137260 | 296 mya | 404 | 51% |
| Xenopus tropicalis | Tropical clawed frog | XP_002935771 | 371.2 mya | 437 | 50% |
| Danio rerio | Zebra fish | XP_706561 | 400.1 mya | 452 | 41% |
| Camponotus floridanus | Florida carpenter ant | XP_011255960 | 782.7 mya | 374 | 38% |

### Evolution rate

When compared to the slow-evolving cytochrome C gene and the fast-evolving fibrinogen gene, gene C14orf80 is also fast-evolving.

## Protein
### General properties

Uncharacterized protein C14orf80 is 426 amino acids long with a molecular weight of 47 kDa. Its isoelectric point is 8.9.

### Secondary structure

Uncharacterized protein C14orf80 is predicted to be entirely composed of alpha helices. Using the program SOUSI-signal, it was predicted that uncharacterized protein C14orf80 does not contain a signal peptide and is a soluble protein.

### Function
#### Domains

Uncharacterized protein C14orf80 has two functional domains. The first domain is the domain of unknown function 4509 and the second is the domain of unknown function 4510. As their naming states the functions of these domains are still unknown.
DUF4509 is located at amino acid 45 to amino acid 228. In this domain of unknown function there is a conserved WLL sequence motif.
DUF4510 is located at amino acid 263 to amino acid 425. In this domain of unknown function there are two conserved sequence motifs: LEA and WMD.

### Post-translational modification

Uncharacterized protein C14orf80 is predicted to have glycation and phosphorylation sites for post-translational modification. Of these sites three are for glycation, eight are for serine phosphorylation and one site is for threonine phosphorylation.

### Subcellular location

Uncharacterized protein C14orf80 is not predicted to be a transmembrane protein. It is mainly localized to the golgi apparatus but has been found in the nucleus and cytoplasm also.

### Interactions

Currently, there are 21 proteins that are predicted to interact with uncharacterized protein C14orf80. These 21 proteins were found using the databases Mentha, BioGRID, STRING, GeneCards and IntAct. Below is a table of a variety of these 21 proteins.
| Interacting protein | Full protein name | Function | Citation |
| DDIT3 | DNA-damage inducible transcript 3 | Induces cell cycle arrest and apoptosis when ER stress | |
| CEBPZ | CCAAT enhancer binding protein | Stimulates transcription from HSP70 promoter | |
| UBC | Ubiquitin C | Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, et al. (May 2012). "Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition". Molecular & Cellular Proteomics. 11 (5): 148–59. doi:10.1074/mcp.M111.016857. PMC 3418844. PMID 22505724. |
| FKBP5 | FK506 binding protein | Immunophilin protein with PPIase | Taipale M, Tucker G, Peng J, Krykbaeva I, Lin ZY, Larsen B, et al. (July 2014). "A quantitative chaperone interaction network reveals the architecture of cellular protein homeostasis pathways". Cell. 158 (2): 434–448. doi:10.1016/j.cell.2014.05.039. PMC 4104544. PMID 25036637. |
| DEF107A | Beta-defensin 107 | Anti-bacterial activity | |
| XAGE1D | Cancer/testis antigen family 12 member 1D | | |
| TRAK2 | Trafficking protein kinesin binding 2 | May regulate endosome to lysosome trafficking of membrane cargo | |
| NRF1 | Nuclear respiratory factor 1 | Transcription factor on nuclear genes encoding respiratory subunits and components of the mitochondrial transcription and replication machinery | |

### Clinical significance

Uncharacterized protein C14orf80 has been associated with tumors in the breast, CNS, endometrium, large intestine, lung, skin, and stomach.