NationalLibraryOfScotland/medical-history-of-british-india huggingface dataset:
CONTENTS
Vol. XXIII, Part II
(June, 1953)
The Publications Committee of the Indian Council of Agricultural Research, India,
takes no responsibility for the opinions expressed in this Journal
Original articles
INDIAN WOOL—II. ASSESSMENT OF WOOL
QUALITY (WITH THREE TEXT FIGURES)
INDIAN WOOL—III. QUALITY OF WOOL
PRODUCED BY SHEEP BREEDS OF INDIA
INDIAN WOOL—IV. QUALITY OF
COMMERCIAL WOOL OF IMPORTANT
MARKETING CENTRES
MAIZE CAKE AS A CATTLE FEED
SEASONAL VARIATION IN CONCEPTION
RATES IN ARTIFICIALLY BRED COWS
B. M. Patel, G. K. Wagle, M. R.
Padhye and B. K. Vaidya
B. M. Patel, G. K. Wagle, T. K.
Desai, M. R. Padhye and B. K.
Vaidya
B. M. Patel, G. K. Wagle, and
M. R. Padhye
Lal Chand Dharmani and G. V.
Karira
S. S. Prabhu and P. Bhattacharya
Abstracts
Review
PAGE.
81
93
113
123
129
135
142
Vol. XXIII, Part II
June, 1953
The
Indian Journal of Veterinary Science
and
Animal Husbandry
Issued under the authority
of
The Indian Council of Agricultural Research
Annual subscription
Rs. 12 or 20s.
Price per part
Rs. 3 or 5s.
PUBLISHED BY THE MANAGER OF PUBLICATIONS, DELHI.
PRINTED BY THE GOVERNMENT OF INDIA PRESS, CALCUTTA, INDIA,
1953
ICAR. 6. XXIII II.
875.
Vol. XXIII, Part III
September, 1953
Indian Journal of Veterinary Science
and
Animal Husbandry
Issued under the authority
of
The Indian Council of Agricultural Research
Annual subscription
Rs. 12 or 20s.
Price per part
Rs. 3 or 5s.
PUBLISHED BY THE MANAGER OF PUBLICATIONS, DELHI.
PRINTED BY THE GOVERNMENT OF INDIA PRESS, CALCUTTA, INDIA
1953
ICAR. 6. XXIII. 3.
875.
CONTENTS
Vol. XXIII, Part III
(September, 1953)
The Publications Committee of the Indian Council of Agricultural Research,
India, takes no responsibility for the opinions expressed in this Journal
Original articles
STUDIES ON THE EFFECT OF DIFFERENT
FODDERS ON THE MILK YIELD AND
ITS COMPOSITION AND MINERAL ME-
TABOLISM IN SAHIWAL COWS.
REPLACEMENT OF PLANT PROTEINS
BY ANIMAL PROTEINS IN THE RA-
TIONS OF BREEDING BULLS AND ITS
EFFECT ON SEMEN PRODUCTION
EFFECT OF MONTHLY CUTS ON THE
CHEMICAL COMPOSITION OF SOME
INDIGENOUS
GRASSES
OF
INDIA
(WITH TWO TEXT FIGURES)
EFFECTS OF SHADING AND SPRINKLING
ON CATTLE
AND
BUFFALOES IN
EGYPT
M . L . Mathur and S. V. Desai
S. S. Prabhu, S. Guha and P.
Bhattacharya
Narindar Nath and N. B. Das
M. T. Ragab, M. A. Ghany and
A. A. Asker
Abstracts
Review
Page
143
175
185
205
217
219
CONTENTS
Vol. XXIII, Part IV
(December, 1953)
The Publications Committee of the Indian Council of Agricultural Research,
India, takes no responsibility for the opinions expressed in this Journal
Original articles
STUDIES ON CALCIUM AND PHOSPHORUS
IN MILK AND FEEDS OF SAHIWAL
COWS FROM PRECALVING PERIOD TO
THE END OF LACTATION (WITH
13 TEXT-FIGURES)
ASSIMILATION OF PHYTIN PHOSPHORUS
BY DAIRY COWS
FREEZING POINT OF COW AND BUFFALO
MILK. IV. EFFECT OF SOME FACTORS
ON THE FREEZING POINT OF MILK
(WITH TWO TEXT-FIGURES)
THE STUDY OF NORMAL BLOOD OF SHEEP
COMPARISON OF FOUR EJACULATES
COLLECTED IN RAPID SUCCESSION
FROM THE INDIAN WATER BUFFALO
(B. BUBALIS)
FERTILITY OF BULL SEMEN PRESERVED
FOR
LONG
PERIODS
ACCURACY OF A SIMPLE METHOD FOR
ESTIMATING THE PERCENTAGE OF
ABNORMAL SPERMATOZOA IN SEMEN
SAMPLES
PADDY-CUM-FISH CULTURE.
PILOT
STUDIES CONDUCTED AT HESSERAGH-
ATTA AND VISWESWARAYA CANAL
FARMS IN MYSORE STATE (WITH
THREE TEXT-FIGURES)
M. L. Mathur and S. V. Desai
M. L. Mathur
C. S. Dharmarajan, R. Venkates-
wara Rao and Noshir N. Dastur
A. F. Hathoot
S. S. Prabhu and U. D. Sharma
S. S. Prabhu, K. D. Bhaya and P.
Bhattacharya
S. S. Prabhu and U. D. Sharma
H. D. R. Iyengar
Abstracts
Reviews
Page
221
243
249
269
273
279
283
289
299
302
Vol. XXIII, Part IV
December, 1953
The
Indian Journal of Veterinary Science
and
Animal Husbandry
Issued under the authority
of
The Indian Council of Agricultural Research
Annual subscription
Rs. 12 or 20s.
Price per part
Rs. 3 or 5s.
PUBLISHED BY THE MANAGER OF PUBLICATIONS, DELHI
PRINTED BY THE GOVERNMENT OF INDIA PRESS, CALCUTTA, INDIA
1954
ICAR. 6. XXIII. 4.
875.
ORIGINAL ARTICLES
THE EFFECT OF FEEDING THYROPROTEIN ON SEMEN
QUALITY AND ON SOME PHYSIOLOGICAL CONDITIONS
OF GOATS
I. EFFECT ON REACTION TIME AND SEMEN CHARACTERISTICS
By D. P. MUKHERJEE, A. ROY and P. BHATTACHARYA,Animal Genetics Section,
Indian Veterinary Research Institute, Izatnagar
(Received for publication on 1 April 1952)
(With Text-figures)
A LTHOUGH spermatozoa are produced throughout the year in farm animals,
the rate of spermatogenesis and the quality of semen are greatly influenced
by climatic changes. Experiments on different species of animals have shown that
the climatic conditions prevailing in certain seasons are conducive to the production
of good quality semen, but that the quality deteriorates very much during other
seasons of the year. In India, high air temperature associated with high relative
humidity and rainfall has an adverse effect on the quality of both semen and blood
[Mukherjee and Bhattacharya, 1947 a and b ; Shukla and Bhattacharya, 1947 a
and b].
Attempts have been made in the United States of America to improve the
semen quality in rams during an unfavourable season by administration of thyro-
xine or thyroactive materials. Berliner and Warbritton [1937] observed that the
administration of thyroxine to rams during the summer improved the quality of
semen. This observation has been supported by Bogart and Mayer [1946],
Warwick et al. [1948] found that rams which tolerated the dosage of thyroactive
protein administered to them produced in the summer semen which was either
equal to or slightly better in quality than that produced by the control group.
But the treatment of rams with thyroactive protein during spring had a deleterious
effect on semen quality. Eaton et al. [1948] reported that thyroprotein (1 and 2
gm.) given to rams daily from May to September did not improve their semen
quality.
In view of the conflicting results obtained by the above mentioned workers
and especially because in India there is a very long period of high environmental
temperature, the present investigation was undertaken to study the effect of the
administration of thyroactive protein on semen quality and ' reaction time ' of
goats during the summer, the most unfavourable period for semen production.
MATERIAL AND METHODS
Experimental animals comprised 14 male goats between 1 to 2½ years of age.
Before the commencement of the experiment they were trained to mount on anoes-
trous female and ejaculate semen in the artificial vagina. After the animals were
properly trained, they were divided into two groups of seven each as evenly as
2
THE EFFECT OF FEEDING THYROPROTEIN ON SEMEN (I) [Vol. XXIII, Part I,
possible on the basis of semen qualities produced during the ' pre-treatment period '
of three weeks.
Throughout the experimental period, the animals were kept under uniform
dietary and managerial regime. The concentrate supplied consisted of the following
ingredients.
Rape cake
Wheat bran
Gram
Indian corn
Crushed barley
Salt
1000 parts by weight
200 „
„
„
600
„
„
„
680 „ „
„
680 „ „
„
28 „ „ „
Three-quarters of a pound of the above mixture was given to each animal, except
goat No. 62 (of the experimental group) and No. 56 (of the control group) which
received one pound per day due to their heavier body weight. In addition, eight
pounds of green pakhar (Ficus infectoria) leaves per head were supplied daily.
They were allowed free grazing for four hours a day. Animals of the experimental
group were fed daily with 1 gm. of thyroprotein (*Protamone) from middle of May
to middle of August, 1949.
Semen was collected from each animal twice every week and each time it was
examined for the following characteristics :—
(1) Volume
(2) Initial motility of spermatozoa
(3) Sperm concentration per ml. of semen
(4) Total number of spermatozoa per ejaculate
(5) Percentage of abnormal spermatozoa
The ' reaction time ' of the animals was also noted. The reaction time which is
meant to express the degree of sex vigour, was determined by the time interval
between the release of the goat near the she-goat and the actual moment of ejacu-
lation. During the treatment period all the above mentioned characteristics of
semen were studied simultaneously. Volume of semen was measured upto 1/100
ml. Initial motility was scored in terms of the criteria recommended by Erb et
al. [1942]. Sperm concentration was determined by the haemocytometer. For
the determination of percentage of abnormal spermatozoa the method adopted by
Mukherjee and Bhattacharya [1947] was followed.
RESULTS
Twelve weeks after the treatment was started one of the goats (No. 52) of the
experimental group died of penumonia. Data regarding this animal for all the
semen characteristics were excluded from the computation of the results.
The weekly average ' reaction time ' and semen quality of the two groups during
the experimental period are presented in Fig. 1. From the figure it is apparent
*Kindly supplied by Mr W. R. Graham, Jr. of Cerophyll Laboratories INC., U.S.A.
March, 1953] D. P. MUKHERJEE, A. ROY AND P. BHATTACHARYA 3
Fig. 1. Weekly average 'reaction time' and semen quality of two groups.
4
THE EFFECT OF FEEDING THYROPROTEIN ON SEMEN (I) [Vol. XXIII, Part I,
TABLE I
Average of reaction time and semen qualities of control and experimental groups of goats
Reaction time (in seconds)
Volume of semen in ml.
Initial motility of spermatoza
Sperm concentration in millions
per ml. of semen
Total number of spermatozoa
in millions
Percentage of abnormal sperma-
tozoa
Pretreatment period
Control group
31.3±3.31
0.54±0.04
4.35±0.11
5368±247.88
2778±242.0
3.35±0.40
Experimental
group
24.26±1.60
0.43±0.05
4.16±0.06
5025.00±296.20
2159±222.0
5.26±0.56
Difference
between
the two
means
7.04
0.10
0.19
343
619
1.91
t. value
1.786
1.617
1.083
0.948
1.855
2.852**
Treatment period
Control group
32.6±2.33
0.63±0.03
4.21±0.04
4322±186.94
2761±219.99
6.66±0.30
Experimen-
tal group
28.77±1.06
0.50±0.03
4.29±0.07
4987±142.23
2287±128.6
9.62±1.01
Difference
between
the two
means
3.9
0.13
0.08
665
474
2.96
t. value
1.429
2.915**
0.992
2.707*
1.769
3.262
** Significant at one per cent.
March, 1953] D. P. MUKHERJEE, A. ROY AND P. BHATTACHARYA 7
thyroprotein per day. It seems that the lack of sexual urge or libido in males born
with hypothyroid condition may be remedied by the administration of thyroprotein.
But the hypothyroid condition created in males by external factors, such as high
air temperature, high relative humidity and high rainfall, does not reduce the sexual
behaviour of the animal. Therefore, the feeding of thyroprotein seems to have
little effect on the sexual activity as measured by the ' reaction time '.
SUMMARY
Observations made on seven goats treated with thyroprotein and seven control
animals for a period of 14 weeks during May to August revealed the following.
(1) Thyroprotein feeding had no significant effect on the ' reaction time ',
initial motility, total number of spermatozoa per ejaculate and percentage of abnor-
mal spermatozoa.
(2) Sperm concentration increased due to thyroprotein feeding.
(3) A highly significant decrease in semen volume was obtained due to the
administration of the drug.
ACKNOWLEDGEMENT
The authors are grateful to Dr. S. Datta, Director, Indian Veterinary Research
Institute, for his kind interest in this work.
REFERENCES
Berliner, V. and Warbritton , V. (1937). The Pituitary and Thyroid in Relation to Sperm Pro-
duction in Rams. Proc. Amer. Soc. Anim. Prod. 30 Ann. Meet. 137
Bogart, R. and Mayer, D. T. (1946). Environmental Temperature and Thyroid Gland Involve-
ment in Lowered Fertility in Rams. Mo. Agr : Expt. Sta. Res. Bull. 402
Erb., R. E., Andrews, F. N. and Hilton, J. H. (1942). Seasonal Variation in Semen Quality of
Dairy Bull. J. Dairy Sci. 25, 815
Eaton, O. N., Schott, R. G., Simmons, V. L. and Franks, A. H. (1948). The Effect of Feeding
Thyroprotein on Semen Characteristics of Rams. J. Anim. Sci. 7, 449
Goulden, C. H. (1939). Methods of Statistical Analysis. Pub. Chapman and Hall, Ltd.
Mukherjee, D. P. and Bhattacharya, P. (1947 a). Seasonal Variation in Semen Quality
and Haemoglobin and Coll Volume Contents of Blood in Bulls (In press). (Abst.) Proc.
Indian Sci. Congr. 3, 215
—— (1947 b). Seasonal Variations in Haemoglobin and Cell Volume Contents of Blood in
Rams and Goats (In press). (Abs.) Proc. Indian Sci. Congr. 3, 3
—— (1949). Study of Spermatozoa from Different Levels of the Male Reproductive Tracts of
the Sheep, Goat and Buffalo. Proc. Zool. Xoc. Bengal. 2, 149
Reinke, E. P. (1946). The Effect of Synthetic Thyroprotein in Sterility in Bulls. The Problem
of Fertility, 1946. Princeton University Press, Princeton, N. J.
Shukla, D. D. and Bhattacharya, P. (1947 a). Seasonal Variation in ' Reaction time ' and
Semen Quality of Sheep (In press). (Abst.) Proc. Indian Sci. Congr. 3, 215
—— (1947 b). Seasonal Variation in Reaction time and Semen Quality of Goats (In press)
(Abst.) Proc. Indian Sci. Congr. 3, 215
Turner, C. W., Mixner, J. P. and Reineke, E. P. (19 43). Thyroprotein for Sterile Goats. Dairy
Goat J. 21, 1
Warwick, E. J., Childs, C. E., Flower, A. E., and Ham, W. E. (1948). Ram Semen Production
and Characteristics as Influenced by Administration of Thyroprotein. J. Anim. Sci.
7, 198
THE EFFECT OF FEEDING THYROPROTEIN ON SEMEN
QUALITY AND ON SOME
PHYSIOLOGICAL
CONDITIONS OF GOATS
II. EFFECT ON SPERM NUTRIENT AND ITS UTILISATION
BY A. ROY, D. P. MUKHERJEE, S. N. LUKTUKE AND P. BHATTACHARYA, Animal
Genetics Section, Indian Veterinary Research Institute, Izatnagar
(Received for publication on 1 April 1952)
With one Text-Figure
A PART from actual fertility tests, no one method can forecast the fertilizing capa-
city of semen. The available methods for the grading of semen are all subjected
and unless several such methods are applied simultaneously the conclusions may be of
'dubious value. In Part I of this series, data on the influence of thyroprotein on
semen quality as determined by microscopical methods were presented. The
data obtained by biochemical methods might further elucidate the efficacy of the
administration of thyroprotein in improving the quality of semen. With the iden-
tification and isolation of fructose as the only glycolysable sugar present in semen
[Mann, 1948], an entirely new method—the fructolysis test—is now available for
the appraisal of semen quality. We have studied the semen quality by this as well
as by another biochemical test—the methylene blue reduction time (MBRT). The
investigation of semen quality by methods based on metabolic activity is of particular
interest in view of the fact that the sperm concentration in a given sample of semen
was inversely related to the concentration of the sperm metabolite contained therein
and that the rate of utilisation of the sperm metabolite was quantitatively related
to sperm concentration [Roy, et al. 1950]. These findings were arrived at by using
ram and buffalo semen. As expected, these findings have been found to hold good
in goats also.
MATERIAL AND METHODS
During the experimental period, the requisite volume of semen required for the
investigation reported in Part I [Mukherjee, et al. 1952] was spared, the rest
was used to estimate (i) the rate of fructolysis and (ii) the time required to reduce
the colour of methylene blue. The methods adopted have been described by
Roy, et al. [1950].
RESULTS
The averages of these seminal attributes, namely the initial fructose content
(IFC), fructolysis and MBRT, together with the results of statistical analysis of data
during the entire experimental period, have been collected in Table I. The statisti-
cal analysis was done by the method of Goulden [1939].
It was found that when the averages for the whole experimental period were
taken into account, the IFC and the rate of fructolysis of the two groups, like sperm
concenration, differed. In the experimental group, there was a significant decrease
in the IFC and a significant increase in the rate of fructolysis. The differences
9
2
10
THE EFFECT OF FEEDING THYROPROTEIN ON SEMEN (II) [Vol. XXIII, Part I,
TABLE I
Summary of statistical analysis of average IFC, rate of fructolysis and MBRT of
semen of goats
IFC (mg./100 ml. of semen)
Fructolysis (mg./100 ml. of semen)
MBRT (minutes)
Control group
945.35±50.40
41.34±2.99
34.77±1.75
Experimantal
group
754.83±61.29
51.70±3.92
31.73±1.90
Difference
between the
two means
190.52
10.36
3.04
t—value
2.42*
2.13†
1.16
*Significant at two per cent level
†Significant
at one per cent level
in the weekly averages, however, of the IFC, rate of fructolysis and MBRT were
not found to be statistically significant. Furthermore, the difference in the MBRT
of the two groups, even when the average for the whole experimental period was
considered, was not significant. In both the groups, weekly variation, the IFC
and MBRT of the semen showed the reverse trends of variation, but the rate of
fructolysis showed the same trend of variation as that of sperm concentration.
(Fig. 1)
TABLE II
Different ranges of sperm concentration and their IFC, fructolysis and MBRT
Range of sperm concentra-
tion (in millions/ml.)
0—2999
3000—3999
4000—4999
5000—5999
6000—7999
8000 and above
Number of
observa-
tions
45
86
72
39
34
13
Mean of
sperm con-
centration
2544.29
±61.39
3497.23
±33.73
4490.72
±21.51
5407.07
±86.65
6789.82
±87.70
9725.61
±400.28
Mean of
IFC (Mg./
100 ml.
of semen)
1294.66
±43.87
1179.15
±40.72
1064.27
±50.28
760.95
±63.59
371.29
±46.60
175.61
±30.40
Mean of
fructolysis
(mg./100 ml.
of semen)
23.57
±1.84
32.59
±1.95
34.13
±2.27
53.28
±4.52
86.76
±4.01
100.00
±0.00
Mean of
MBRT
(minutes)
45.53
±2.56
41.55
±2.18
33.42
±1.95
28.79
±3.19
13.24
±1.59
9.37
±1.50
March, 1953] A. ROY, D. P. MUKHERJEE, S. N. LUKTUKE AND P. BHATTACHARYA 11
Fig. 1. Per lines marked * have been dealt in part 1 of the present series
12 THE EFFECT OF FEEDING THYROPROTEIN ON SEMEN (II) [Vol. XXIII, Part I,
In Table II, the IFC, the rate of fructolysis and MBRT have been arranged as
they were found to stand in relation to various levels of sperm concentration. The
groupings of the various ranges of sperm concentration as shown in this table were
arbitrary. It will be evident from the table that with the increase in the sperm
concentration per unit volume, (i) the IFC per 100 ml. decreased, (ii) the rate of
fructolysis showed a concomitant rise, and (iii) the time required to reduce methy-
lene blue became less. Correlation co-efficients between sperm concentration and
the three semen characteristics, as worked out by the method described by Goulden
[1939] are given below:
Correlation between sperm concentration per unit volume of semen and
(i) IFC per 100 ml. of semen
(ii) MBRT
(iii) Rate of fructolysis
—0.968
—0.958
+0.958
These correlations are statistically highly significant.
TABLE III
Variations in sperm concentration, IFC, fructolysis rate and MBRT of semen in
relation to different grades of initial motility of spermatozoa
Sperm concentration (in mil./ins./ml.
of Semen)
IFC (in mg./100 ml. of semen)
Fructolysis (in mg./100 ml.)
MBRT (in minutes)
Different
+++
3119.83
±173.94
(24)
1367.62
±65.12
(24)
24.00
±3.04
(24)
42.29
grades of
motility
++++
3903.84
±83.04
(166)
1153.78
±29.43
(166)
30.57
±1.15
(166)
39.09
initial
++++
6194.37
±146.59
(89)
544.11
±38.53
(89)
69.50
±3.36
(89)
21.84
Difierence
between
the means
of +++
and
++++
784.0
213.84
6.57
10.20
t—value
3.43**
2.62*
2.014
2.36**
Difference
between
the means
of+ + + +
and
+++++
2290.58
609.67
38.93
17.25
t—value
14.6**
12.4**
13.3**
6.62**
*Significant at five per cent level
**Significant at two per cent level
Figures in brackets are numbers of samples
In Table III the averages of sperm concentration, IFC, fructolysis and MBRT
have been arranged as they stood in relation to the different grades of initial motility
of spermatozoa. From the table it is evident that with the increase in initial motility
of spermatozoa there was (i) an increase in the sperm concentration and in the rate
of fructolysis. and (ii) a decrease in the IFC and in the MBRT of the semen. It is
also evident from the table that the largest number of semen samples showed an
initial motility of ++++. The difference in sperm concentration and MBRT
between samples with +++ and ++++ motility were highly significant, but the
March, 1953] A. ROY, D. P. MUKHERJEE, S. N. LUKTUKE AND P. BHATTACHARYA 13
difference in the IFC and rate of fructolysis were only significant at five per cent and
two per cent levels, respectively. Differences between samples with ++++ and
+++++ initial motility were highly significant in all the four characteristics.
DISCUSSION
Since they discovered that fructose is the only glycolysable sugar present in
semen, Mann and his collaborators have investigated the possibility of its utilisation
from various aspects. In one of their communications [Mann, 1948] it was reported
that the 'fructolysis index' of a good semen sample obtained from healthy bulls
with good fertility records was between 1.4 and 2.0 mg./10 sperm/ hour. Further,
the semen samples obtained from animals with poor fertility records were character-
ised by a low content of fructose. Besides these, the rate of fructolysis now appears
to be the most satisfactory method for assessing not only sperm activity but density
as well [Rothschild, 1949]. In an endeavour to put the relation of sperm concen-
tration to the degree of fructolysis on a quantitative basis, we found in the present
investigation that the ranges of sperm concentration and fructolysis were significantly
correlated and that the IFC and sperm concentration were also similarly related.
This higher correlation coefficient, therefore, indicated that for the estimation of
sperm concentration a high accuracy existed in the measurement of IFC and fruc-
tolysis. This was found to be so, not only in goats but also in rams and buffaloes
[Roy, et al. 1950].
Semen samples with higher sperm concentration and higher initial motility
have been found to reduce methylene blue much faster than those with lower sperm
concentration and lower initial motility. This finding agrees with those reported
by Van Demark, et. al. [1945] in bull semen. Warwick et al. [1948] found that
semen, obtained from rams receiving 0.5 g. of thyroprotein, had high initial motility
and reduced methylene blue faster than samples obtained from either the control
group receiving 1.5 gm. of thyroprotein. The results obtained in this experiment
show that the average MBRT of the experimental group did not differ significantly
from that of the control group. This is due possibly to the fact that MBRT, like
initial motility of the spermatozoa, is not a precise method of appraising of semen
quality and can only be used for differentiating semen samples of widely varying
qualities. When the variation is small, the evaluation of quality even within
reasonable limits by the use of this method, in our experience, is a difficult task
indeed.
SUMMARY
Thyroprotein feeding had no significant effect on methylene blue reduction
time of semen.
Initial fructose content decreased and the rate of fructolysis of semen increased
significantly due to thyroprotein feeding.
Highly significant negative correlation coefficients were found between sperm
concentration and initial fructose and methylene blue reduction time. There was
highly significant positive correlation coefficient between sperm concentration
and rate of fructolysis of semen.
3
14 THE EFFECT OF FEEDING THYROPROTEIN ON SEMEN (II) [Vol. XIII, Part I,
ACKNOWLEDGMENT
The authors are grateful to Dr S. Datta, Director, Indian Veterinary Research
Institute, for his kind interest in the work.
REFERENCES
Goulden, C. H. (1939). Methods of Statistical Analysis. Pub. Chapman and Hall, Ltd.
Mann, T. (1948). J. agric. Sci. 38, 323
Mukherjee, D. P., Roy, A and Bhattacharya, P. (1952). J. vet. Sci. (In press)
Rothschild, Lord (1949). Nature 163, 358
Roy, A., Luktuke, S. N., Bhattacharya, S., and Bhattacharya, P. (1950). Indian. J. dairy
Sci. 3, 161
Van Demark, N. B., Mercier, E. and Salisbury, G. W. (1945). J. dairy Sci. 28, 121
Warwick, E. J., Childs, C. E., Flower, A. E. and Ham, W. E., (1948). J. anim. Sci. 7, 198.