NationalLibraryOfScotland/medical-history-of-british-india huggingface dataset:
21
to note in this connection that spontaneous amœbic dysentery, and also liver
abscess in animals, have been noted by Kartulis, 1 Castellani2 and ourselves.
We have been able to cultivate amœbæ from the stools of different animals. The
following table shows the kind and number of animals examined and the presence
or absence of amœbæ in the stools :—
No.
Normal animal.
No. of
normal
animals.
Amœbæ
in
1
Monkeys (Macacus sp.?) .
53
53
2
Kittens . . . .
6
6
3
Rabitts . . . .
47
43
4
Guinea pigs . . .
54
49
5
Rats . . . . .
74
71
6
Flies, house (Musca domestica)
contents of alimentary tract.
67
61
It was considered that a detailed study of the amœbæ found in normal ani-
mals did not come within the scope of the enquiry, hence no in vestigations have
been made by us on their morphology and life-cycle. It is interesting to note that
Miss Robertson inclines to the view that the amœba cultivated from the stools of
the monkey differs somewhat from the amœba found in dysentery and tap water.
We kept about 50 normal monkeys under observation, examinirg their stools at
frequent intervals to determine the presence or absence of amœbæ and other para-
sites, as well as recording their weight and general condition during the period. In
Table III, Appendix, the results of our investigations on the monkeys under obser-
vation, as well as a record of the monkeys which died with s ymptoms of dysentery,
are given. It was important to make a careful study of normal monkeys in regard
to the occurrence of amœbæ in their alimentary tract, because we were using these
animals for feeding experiments, and they were used, also, by Musgrave and Clegg
and Noc in their investigations on amœbic dysentery in Manilla and Cochin China
respectively. It will be seen from a study of Table III, Appendix, that amœbæ
were present in the stools of normal monkeys ; they were not detected every time
the stools were examined, but at irregular intervals. This is a point of considerable
importance, because if one or two examinations only had been made the monkey
might have been regarded as free from amœbæ and used for experiment. The
presence of parasitic amœba in the alimentary tube of healthy monkeys, which
1 Kolle und Wassermann, 1907.
2 Journal of Parasitology, June 1907.
22
apparently closely resembles the amœba found in dysentery in man, introduces a
serious experimental difficulty. The problem was complicated still further by the
fact that, whilst under observation, some of the normal monkeys with amœbæ in
their stools lose weight, develop symptoms of dysentery, and die, and the post
m ortem shows lesions in the large intestine.
Hence it will be evident that the results obtained from feeding monkeys
with amœbic cultures must be accepted with caution. The fact, however, that
apparently healthy monkeys, in whose stools amœbæ have been determined,
may develop symptoms of dysentery and die, is one of considerable interest and
importance in connection with the etiology of the disease, although it does not
establish conclusively the causal relationship between amœbæ and the symp-
toms.
In regard to the occurrence of spontaneous amœbic infection in animals ar in-
teresting observation was made by one of us (E. D. W. G.). A cat was being fed with
a particular kind of rice for the purpose of an experiment not connected with the
dysentery investigation. It showed no symptoms of dysentery during life, but
became very much emaciated. It was unable to stand ; the hind legs were para-
lysed. It died on 26th February 1910. Post mortem, the stomach showed
" patches " in several places, especially towards the duodenum and on the
lesser curvature. These " patches " were hæmorrhages under the mucus mem-
brane. In some places actual erosion of a superficial nature had taken place.
The affected portions of the stomach were prepared, embedded in paraffin and
cut. The sections were stained with methylene blue and eosine. On examining
the sections an interesting appearance was noted (vide Plate III, Fig. 1) ; at one part
of the section blood pigment was observed in the submucosa, near to this the
mucous membrane was depressed, and in the epithelial layer a number of struc-
tures, taking the eosine stain strongly, were observed (Plate III, Fig. 2). Under a
high power it was noted that the eosine staining bodies had the following struc
-ture : (1) a number of circular bodies varying in size and staining readily with the
eosine ; (2) a blue stained body, the nucleus, with a central chromatine mass. The
nucleus is always placed superficially to the circular bodies ; (3) 2 and 3 lie in clear
hyaline material. Specimens were sent to Professor E. A. Minchin, F.R.S., for
opinion, and he kindly examined them and gave the following very interesting
Report :—" My impression of these bodies is that they are amœbæ of the histolytica
type and that the eosinophil bodies are red corpuscles in various stages of digestion
and absorption. Jürgens figures exactly similar appearances in dysenteric amœbæ,
stuffed out with red blood corpuscles greatly diminished in size. The chief ob-
jection to regarding them as dysenteric amœbæ is their occurrence in the stomach.
I never heard of them anywhere forward of the large intestine, but Jürgens in-
fected his cats per rectum ; possibly if infected per os the amœbæ might start in
23
the stomach. On the other hand the two chief characteristics of the histolytica
type are the superficial nucleus, placed at the boundary between ectoplasm and
endoplasm, and the greatly developed ectoplasmic layer : both these features are
seen. The blood corpuscles in process of digestion would be in the endoplasm. I
think therefore your cat had got an infection of amœba histolytica or some closely
allied form. If they are not that, I don't know what they are. I certainly do
not think they are any normal eosinophil cells of the animal."
(f ) Experimental Observations on Animals.
The following table shows the results of experiments on cats and dogs with
stools from dysentery cases and pus from liver abscess in which the presence of
araœbæ were determined by microscopic examination :—
Serial
No.
Material used and source. Amœba. Animal.
Mode of injec-
tion.
Result.
1
Mucus from dysentery case . Present Kitten . Intrarectal
. Animal killed after two days
— intestine healthy.
2
Scrapings from an ulcerated
cæcum of fatal case of dy-
sentery.
Do.
Pup .
Ditto
.
Killed 15 days later—
intestine healthy.
3
Scrapings from liver abscess
wall.
Do.
Do.
Ditto
. Killed 20 days later—in-
testine and liver healthy.
4
Mucus from dysentery case .
Do.
Do.
Ditto
. Remained healthy.
5
Mucus from dysentery case .
Do.
Do.
Di t t o
.
Ditto.
6
Mucus from dysentery case .
Do.
Do.
Di t t o
.
D i t t o .
7
Mucus from dysentery case .
Do.
Do.
D i t t o
.
Ditto.
8
Mucus from dysentery case .
Do.
Do.
Di t t o
.
Ditto.
9 Mucus from dysentery case .
Do.
Do.
Subcutaneously Died two days later; much
discoloration at site of in-
jection. No amœbæ pre-
sent.
From this table it will be seen that the experiments on cats and dogs gave
negative results. Noc in his experiments on young cats with cultures of the
amœbæ of Cochin China obtained negative results also. He considers this may be
due to the acidity of the contents o f the large intestine of these animals.
(g) Experiments with Monkeys.
Both Musgrave and Clegg in Manilla and Noc in Cochin China made use of
monkeys in their experimental studies on amœbæ. Whilst Musgrave and Clegg,
on the one hand, claim to have produced true amœbic infections in monkeys by
feeding them on cultures, Noc, on the other hand, who has not succeeded in pro-
24
ducing definite lesions in monkeys by feeding them with cultures of amœbæ, points
out that dysenteric symptoms are not rare amongst monkeys, and adds that
the bloody mucus, being a medium rich in red blood corpuscles and serous fluid,
the amœbæ rormally present develop in it. He is inclined to consider that the
true cause of the dysentery in monkeys is not amœbic. In conclusion he asks the
question, whether in the investigation of Musgrave and Clegg there was a true
primary amœbic infection in monkeys at all. As we have already pointed out,
amœbæ resembling those cultured from dysenteric stools of man can be cultivated
from normal monkeys, and further, that these animals suffer from symptoms
of dysentery which in some cases end fatally.
Hence in using monkeys for the production of experimental amœbic dysentery
it is necessary to be aware of this fact, otherwise erroneous conclusions may be
arrived at. We have carried out a number of feeding experiments in monkeys with
cultures of amœbæ ; details of some of these are given below. We have not been
able to convince ourselves that we have induced a true amœbic dysentery in
monkeys, and in this respect we are in agreement with Noe. Some of the animals
have died with ulceration of the large intestine, which may have been of amœbic
origin, but the fact that uninfected monkeys with amœbæ in their stools may die
and show alterations in the large intestine renders the experimental results difficult
of interpretation. From what has been said above it will be readily understood
how complicated the whole subject is, rendering conclusive experimental proof of
the pathogenicity of different species of amœba very difficult. In this connection,
however, the quotation from Doflein's work (p. S of this Report) is of interest.
The following is an account of some of the feeding experiments carried out on
monkeys :—
Ex. 7.—MONKEY.
The strain of amœba used in this experiment was cultivated from the stools
of a case of dysentery No. 8250 Private A, on May 10, 1909.
May 13th, 1909.—Two small petri plates of this strain, which showed many
cysts, were emulsified with 0.75 NaCl, which had been rendered distinctly alkaline
; each 100 c.c. contained 1 c.c. normal NaoH. This emulsion was introduced into
the stomach of this monkey by means of a catheter. Weight of monkey 3,200
grams.
June 29th, 1909. —Two small plates 14th and 15th passage emulsified and
given as before.
July 19th, 1909.—An emulsion of two plates 20th and 21st passage of the
same strain of amœba showing many cysts was made in the same way and intro
-duced into the stomach of this monkey.
August 2nd.—Since July 28th this monkey has had frequent small stools of
bloed and mucus : died this morning. Post mortem.—Weight 2,760 grams. On
25
opening the abdomen patches of deep injection were visible in the large intestine,
most marked in the transverse colon, sigmoid and rectum. The omentum is
firmly adherent to the transverse colon. Slight diffuse injection of the small
intestine up to the duodenum. On opening the large intestine extensive ulcer -
ation is found ; the ulcers are very irregular, undermining the mucus membrane.
Small intestine. —Slight congestion, no ulceration. No worms in alimentary tract.
Liver and other organs show no noteworthy change. No motile amœbæ could
be seen in a scraping from the ulcer, but a culture gave an abundant growth of
amœbæ.
The following table shows the presence or absence of amœbæ in the stools
and the weight of the monkey :—
No. of
Monkey.
Weight (grams).
Stools.
AMŒBA.
Microscope.
Cultivation.
— —
7 3,200—13th May 1909 .
. .
29th April 1909 . 13th May 1909.
—
—
3,280—17th May 1909. No diarrhœa .
. 30th April 1909
Trichomonas.
. 17th May 1909.
—
—
3,300—24th May 1909 .
Ditto
.
. 7th May 1909
. 24th May 1909.
—
—
3,420—31st May 1909 .
Ditto
.
. 17th May 1909
. 14th June 1909.
—
—
3,400—7th June 1909 .
Ditto
.
. 24th May 1909
. 19th June 1909.
—
—
3,390— 14th June 1909. Ditto
.
. 14th June 1909. . 28th June 1909.
—
—
3,390—21st June 1909 .
Ditto
.
. 19th June 1909. . 5th July 1909.
—
—
3,350—28th June 1909. .
Ditto
.
. 28th June 1909
. 16th July 1909.
—
+
3,400—5th July 1909 .
Ditto
.
. 5th July 1909
. 28th July 1909.
—
+
3,370—12th July 1909 .
Ditto
.
. 16th July 1909
Trichomonas.
. 29th July 1909.
—
3,300—19th July 1909 .
Ditto
.
. 28th July 1909.
Trichomonas.
—
3,230—26th July 1909 .
Ditto
.
. 29th July 1909.
Trichomonas.
28th July 1909—Diar-
rhœa and blood mu-
cus.
29th July 1909— Do.
30th July 1909— Do.
31st July 1909— Do.
2,760—2nd August 1909,
died.
REMARKS.—This experiment is a suggestive one. The ulceration of the large intestine of this monkey was well marked.
E
26
Ex. 8.—MONKEY.
May 25th, 1909. —The strain of amœba used in this case was cultivated from
the stools of a case of dysentery, a Hindu male, aged 35, on May 21st, 1909, the
amœbæ having been previously determined in the stools by microscopic examina-
tion. An emulsion of the culture, which contained many cysts, was made with
alkaline 0.75 NaCl, and this was introduced into the stomach of the monkey by
a catheter.
June 7th, 1909. —The monkey passed blood in its stools.
August 24th, 1909.—6 c.c. of Tinct. opii were injected into the peritoneal
cavity of this monkey.
September 2nd. —Animal died. Post mortem. —Body emaciated. No fluid in
the peritoneal or pleural cavities. Large intestine. —Mucus membrane appears to
be congested and necrosed in parts. Ulceration present about the middle of its
course. Liver and other organs show no noteworthy change.
The following table shows the presence or absence of amœbæ in the stools
and the weight of the monkey :—
No. of
Monkey.
Weight (grams).
Stools.
AMŒBA.
Microscope.
Cultivation.
—
—
8 3,100—1st May 1909 .
..
29th April 1909
.
17th May 1909.
—
—
3,180—10th May 1909 .
. .
30th April 1909
Trichomonas.
. 20th May 1909.
—
—
3,280—17th May 1909 .
. .
7th May 1909
.
22nd May 1909.
—
—
3,300—19th May 1909 .
. .
17th May 1909
.
24th May 1909.
—
+
3,350—22nd May 1909 .
. .
19th May 1909
.
7th June 1909.
—
—
3,300—24th May 1909 .
..
22nd May 1909
.
14th June 1909.
—
—
3,350—31st May 1909 . No diarrhœa
.
. 24th May 1909
Trichocephalus.
. 21st June 1909.
—
—
3,360—7th June 1909 . Diarrhœa with bloody
stool.
7th June 1909
. 28th June 1909.
—
—
No diarrhœa
. . 14th June 1909
. 5th July 1909.
—
—
8th June 1909 .
. 21st June 1909
.
16th July 1909.
—
—
9th June 1909 .
. 28th June 1909
.
10th August 1909.
27
No. of
Monkey.
Weight (grams).
Stools.
AMŒBA.
Microscope.
Cultivation.
—
+
3,400—14th June 1909 . No diarrhœa .
. 5th July 1909
. 23rd August 1909.
—
+
3,380—21st June 1909 .
Ditto
.
. 16th July 1909
Trichomonas.
. 26th August 1909.
—
3,370—28th June 1909 .
Ditto
.
. 29th July 1900.
—
3,320—5th July 1909 .
Ditto
.
. 10th August 1909.
—
3,250—12th July 1909 .
Ditto
.
. 23rd August 1909.
3,230—19th July 1909 .
Ditto
.
. 26th August 1909.
Trichomonas.
3,160—26th July 1909 .
Ditto.
3,110—2nd August 1909
Ditto.
3,070—9th August 1909
Ditto.
3,030—16th August 1909
Ditto.
2,990—23rd August 1909
Ditto.
2,260—1st September
1909.
Ditto.
Died 2nd September
1909.
By way of contrast we submit the histories of monkeys which never had
been fed on amœbic cultures, amœbæ being present naturally in their stools.
No. 13.—MONKEY.
30th June 1909. —Amœbæ cultivated from the stools of this monkey.
1st July 1909. —Animal has diarrhœa and has been steadily losing weight.
27th July 1909.—Died. Post mortem. —Body very emaciated. No fluid in
the peritoneal or pleural cavities. Large intestine.—Congested throughout its
whole length. In the upper half numerous sub-mucus hæmorrhages. Raised
areas of bluish gelatinous appearance running transversely. Just below the edge
of the cæcal valve one of the raised areas has broken down and formed an ulcer.
Mesenteric glands enlarged. No worms in the alimentary tract. Liver normal,
other organs show no noteworthy change.
E2
28
The following table shows the presence or absence of amœbæ in the stools, and
the weight of the monkey :—
No. of
Monkey.
Weight (grams).
Stools.
AMŒBA.
Microscope.
Cultivation.
—
—
13 1,650—26th May 1909 .
..
26th May 1909
Omit strongyloides.
26th May 1909.
16th June 1909.
—
—
1,350—31st May 1909 . No diarrhœa .
. 16th June 1909
. 22nd June 1909.
—
+
1,330—7th June 1909 .
Ditto
.
. 22nd June 1909
. 30th June 1909.
—
+
1,270—14th June 1909 .
Ditto
.
. 30th June 1909
Trichomonas.
. 1st July 1909.
—
+
1,310—21st June 1909 .
Ditto
.
. 1st July 1909
Trichomonas.
. 6th July 1909.
—
+
1,210—28th June 1909 .
Ditto
.
. 6th July 1909
Trichomonas.
. 7th July 1909.
—
+
1st July 1909—Diar-
rhœa; no blood.
7th July 1909
Trichomonas.
. 19th July 1909.
—
1,120—5th July 1909 . No diarrhœa .
. 19th July 1909.
Trichomonas.
1,000—12th July 1909 . 6th July 1909—Diar-
rhœa; no blood.
920—17th July 1909 . 7th July 1909
—Diar-rhœa.
Died 20th July 1909 .
8th July 1909—Do.
10th July 1909—Do.
No. 42.—MONKEY.
17th August 1909. —Amœba cultivated from the stools of this monkey.
21st August 1909. —Monkey has diarrhœa.
26th August 1909. —Monkey has been losing weight, is now emaciated and
very weak. Diarrhœa still present. Killed by chloroform. Post mortem. —Body
much emaciated. No fluid in peritoneal or pleural cavities. Large intestine.—
Whole mucus membrane diffusely congested : below the ileo-cæcal valve a patch of
deep congestion with sub-mucus hæmorrhage : about the middle of the large in-
testine is a gelatinous protrusion, the size of a split pea. Mucus membrane in places
is superficially necrosed, no actual ulceration, no worms in the alimentary tract.
Small intestine appears healthy. Liver healthy ; other organs show no noteworthy
change. Scraping from the necrosed portion of the mucus membrane of the large
intestine shows no amœbæ microscopically : amœba recovered by cultivation.
29
The following table shows the presence or absence of amœbæ in the stools, and
the weight of this monkey :—
No. of
Mon-
key.
Weight (grams).
Stools.
AMŒBA.
Microscope.
Cultivation.
— +
42 1,670—13th August 1909 . No diarrhœa . . 17th August 1909 . 17th August 1909.
Trichomonas.
— +
1,640—16th August 1909 .
Ditto . . 21st August 1909 . 21st August 1909.
— +
1,409—23rd August 1909 .
21.8
23rd August 1909 . 23rd August 1909.
Trichomonas.
Died 26th August 1909 .
2 3 . 8
REMARKS . —Th e s e t wo mo n k e y s f o r m e x c e l l e n t c o n t r o l o b s e r v a t i o n s t o t h e t wo p r e v i o u s f e e d i n g e x p e r i me n t s . I n a l l f o u r
mo n k e y s a mœb æ we r e c u l t i v a t e d f r o m t h e s t o o l s . I n a l l f o u r p a t h o l o g i c a l c h a n g e wa s o b s e r v e d i n t h e l a r g e i n t e s t i n e o n post mortem
examination.
The need for caution in regard to drawing conclusions from the feeding experi-
ments on monkeys with amœbic cultures is obvious. In Appendix, Table III, the
records of our observations on a large number of healthy monkeys are given.
GENERAL RESUMÉ.
Before leaving the question of amœbæ in relation to the etiology of dysentery
and liver abscess it may be well to summarise the results very briefly. In the
first place our findings very closely follow those of Noc in Cochin China. The
amœbæ cultivated by us from the stools of dysentery cases and pus from liver
abscesses, although resembling the Ent. histolytica at one phase of its life-cycle,
differs from it particularly in regard to cyst formation. Ent. Coli has not been
cultivated by us. The establishment of the pathogenicity of amœbæ by animal
experiment is difficult and the results are uncertain. A further detailed study of
the life-cycle of parasitic amœbæ of man by trained protozoologists is required,
as lacunæ exist in the description of the classical Ent. histolytica.
V.—Clinical Study of Amœbic Dysentery and Liver Abscess.
Whilst the etiology of amœbic dysentery was being investigated a careful
clinical record was made of each case, which included repeated blood counts, the
examination of the blood for parasites, the taking of temperature records, observing
the effects of treatment, etc., etc. In this way important data were accumu-
lated, and in this section the results of these researches are set forth.
30
(a) BLOOD CHANGES IN AMŒBIC DYSENTERY AND HEPATITIS.
Total and differential counts of the leucocytes were made in a number of cases,
and this was repeated generally on several occasions throughout the disease. Thus
it was possible to determine the leucocytic formula in amœbic dysentery—a ques-
tion, as will be shown later, of great practical as well as scientific interest.
The total number of red blood corpuscles was determined also.
The following table shows the total number of white and red blood corpuscles
per c.m.m. of blood, the percentage of the various white blood corpuscles, the
presence or absence of malarial parasites in the blood, and the presence or absence
o f a mœb æ i n t h e s t o o l s i n d y s e n t e r y c a s e s : —
Serial
No. Name. Age. Sex. Race.
Occupation.
Number
of days in
hospital
and
result
Temperature.
Date
of
examination.
"
R. B. C. W W. B. C.
PERCENTAGES.
Blood.
Malarial
paras.te.
STOOLS.
P. N. S. M. L. M. E.
Character.
____
Amœbæ.
1 A. K.
28
M
E Sepoy, 109th Infy.
41 days
R.
Normal
27th March 1909
64 18.5 14
3.5
—
±
(M)
2 A.
23
M
E Private, 1st Glosters 53 days
It.
99.6 to 104
14th May 1909.
9,650
—
+
(C)
21st May 1909.
8,080 63
30
6.5 0.5
25th May 1909.
11,400 71
24
3.5 1.5
29th May 1909.
12,000
2nd June 1909
11,960
22nd June 1909
67
26.5 3
3.5
3 A. F.
29
M
B Cook .
. . 6 days
Normal
16th June 1909. 3,990,000 28,400 83
10.5 5
1.5
—
21st June 1909.
10,400 73
21
3
3
+
4 B. G.
40
M
H Coolie .
.
7 days
D.
96 to 100
3 1 s t M a r c h 1 9 0 9
4,540,000 12,000 80
8.5 10
0.5
—
(C)
+
5 B. T.
24
M
H Constable .
4 days
R.
Normal
15th April 1909 4,740,000
8,300 57
28.5 4.5 10
—
(M & C)
+
(M & C)
6 B. S.
31
M
H
Do
. 4 days
R.
Do
2nd May 1909.
10,080 53
28
17'5 1.5
—
Blood and
mucus.
+
(C)
C.
20
31
E
53 days
sick leave.
Normal to 100 17th May 1909.
35,240 86'5 10
2
1.
—
Blood
mucus and
+
18th Play 1909.
33,850 81.5 12
5.
1
(C)
20th Play 1909.
32,400
22nd May 1909.
30,370
26th May 1909.
22,130
28th Play 1909.
17,000
1st June 1909.
14,860 80
16
3
1
3rd June 1909.
13,480 79
16
5
0
5th June 1909.
14,400
7th June 1909.
15,920
9th June 1909.
14,500
13th June 1909.
10,000 57
37'5 3
2.5
17th June 1909.
67
29
2.
1.
19th June 1909.
15,640 51
43
5.
0.
Serial' No . Name. Age. Sex. Race.
Occupation.
Number
of days in
hospital
and
result.
Temperature.
Date
of
examination.
R. B. C. W. B. C.
_
PERCENTAGES.
Blood.
Malarial
parasite.
5100L9.
P. N. S. M.
L. M.
____
E.
Character. Amœbæ
21st June 1909.
10,400 55
42
2
1
23rd June 1909.
12,400 48.5 46.5 3
2
25th June 1909.
12,000 49.5 45.5 3.5 1.5
27th June 1909.
10,760 47
44
5.5 3.5
R.
1st July 1909. 3,625,000
9,640 47
45
5.5 2.5
9 P.T.C. 26
M
E 2nd K. E. B.
58 days Normal
7th June 1909. 4,376,000
11,900 97
25
5
3
—
Mucus and
little blood.
+
(C)
18th June 1009.
11,120
60 36
2.5
1.5
10 G. K.
25
M
H Coolie
11 days
R.
97 to 101
14th April 1909. 3,280,000
7,100 65
15.5 19.5
—
Mucus and
blood.
+
(C)
16th April 1909.
5,600
11 D.A.
28
M Goa Student
34 days
R.
Fever
4th May 1909.
15,200 50
31
17
2
—
6th May 1909.
22,190 52
31
15.5 1.5
8th May 1909.
24,680
10th May 1909.
27,670 62.'5 26
11
0.5
Mucus
+
(C)
12th May 1909.
16,560 63
33
3
1
13th May 1909.
20,570 60.5 33
5.5 1
14th May 1909. 6,100,000 19,920
15th May 1909.
18,410 51
40
5
4
17th May 1909.
15,500 47.5 45.5 4
3
19th May 1909. 5,620,000 16,050 58.5 34.6 4.5
2 . 5
23rd May 1909.
15,700
26th May 1909.
14,620
28th May 1909.
12,900
20th May 1909.
10,400
1st June 1909.
13,000
4th June 1909.
9,280
12
G.
36
M H Servant
14 days
R.
Normal
to 99.
21st May 1909.
36,720 75 20
3
2
—
Mucus and
blood.
+
(C)
23rd May 1909 6,416,000 26,140
CAD