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horse out of work, and he had not been out of the station within the past twelve months or more.
It was therefore considered in every way a good subject for this experiment. Blood was drawn
from the jugular vein of case 4 (apendix D), and three separate drams of it injected as soon
as possible with a hypodermic syringe into three separate parts of the neck The pulse of the
pony this morning was 40, and the temperature 99 3/5. This evening, just before the inoculation,
the temperature was 100 2/5, pulse 44. I may remark that I find about one degree difference in
temperature, morning and evening, common in healthy horses.
September 3Oth—Morning.—Pulse at the jaw almost indistinct. Heart pulse very
intermittent and irregular, about 35 or 40 (made out by auscultation), temperature 97 1/5 (this
observation was repeated). Blood examined under the microscope, found normal. Slightly
off feed.
October 1st—Morning.—Pulse as yesterday, temperature 99 2/5. Feeding well.
October 2nd—Morning.—Pulse 40, intermittent, but not so irregular; temperature 103 3/5.
Feeding well.
October 3rd—Morning.—Pulse same as yesterday, temperature 101 4/5. Eyes suffused.
Feeding.
October 4th—Morning.—Pulse 44, not intermittent, quite full and distant at the jaw,
temperature 104 (this observation was repeated). Visible mucous membranes becoming of a
dirty yellow color such as is characteristic in surra. Petechiae of the conjunctiva. Blood
examined and the parasites found swarming in it and very active. They are more slender and
transparent than they are in advanced case. It is now only 4½ days since the inoculation.
Feeding well.
October 5th—Morning.—Pulse 46, not intermittent; temperature 103 1/8. The appearance of
the eyes is very characteristic of surra. Feeding well.
October 6th—Morning.—Pulse 56„ almost indistinct at the jaw, but not intermittent.
Visible mucous membranes are very yellow. There is no oedema except of the eyelids. Tem-
perature 103 Feeding well.
October 7th—Morning.—Pulse 44, temperature 100 4/5. Feeding.
October 8th—Morning. —Pulse 45, temperature 99. Feeding.
October 9th—Morning.—Pulse 44, temperature 100. Feeding.
October 16th—Morning.—I was absent during the past week, visiting outposts. I am told
the pony has continued to feed well, but he has been much reduced in condition since he was
inoculated. Pulse 40, temperature 100 1/5. There is hardly any oedema of the legs. The
visible mucous membranes are of a dirty yellow color with petechiae continuing on the
conjunctiva. Blood examined, the parasites found swarming, but the corpuscles are not
abnormal in any way.
October 18th—Morning.—Though this pony was not so much reduced as the others which I
destroyed for post-mortem examination, yet there appears no hope of his recovery, and he was
already useless from the " kammari" before the inoculation. I am concluding my observations
here to-day and purpose returning to Simla to-morrow, therefore I had this case destroyed in
order to examine him after death.
Post-mortem examination.—There was slight effusion of clear serum in the abdominal cavity,
and fluffy deposit of lymph on the parietal peritoneum. No sign of inflammation. The liver,
kidney, spleen, heart and lungs were healthy, as were also the stomach and the intestines, which
were opened and examined throughout.
Remarks.—This case has fully confirmed the experiment of case 4 with regard to the disease
being communicable by the subcutaneous injection of the blood containing the parasite, and it
even more strikingly illustrates the short stage of incubation. The parasites were found
swarming in the blood of this case, and the visible mucous membranes assuming the appearance
peculiar to surra four and-a-half days after the inoculation. The next point of note is the shock
the nervous system received a few hours after the inoculation, as shown by the intermittent
pulse, the difficulty of feeling the pulse except at the heart by auscultation, and the lowering of
the temperature. It is surprising how little the appetite was affected, and how rapidly the animal
wasted while he ate as well as he did in health.
No local irritation followed where the hypodermic syringe punctured the skin in the
operation of inoculating.