NationalLibraryOfScotland/medical-history-of-british-india huggingface dataset:
REPORT BY SURGEON-CAPTAIN J. F. EVANS.
227
I would suggest that the other samples of bhang examined, i.e., Khandesh, Amballa,
Sholapur, and Satara bhang were obtained either from cultivated plants or contained an acci-
dental admixture of ganja. This would serve to account for their possessing greater physiolo-
gical activity than the standard bhang.
In conclusion, I have nothing to add to the foregoing remarks except that the ex-
periments recorded, together with the necessary observations, occupied the greater part of
each day during two months, apart altogether from the preparation of the alcoholic extracts
half of which were prepared by Babu Ram Chandra Datta, 1st Assistant to the Chemical
Examiner to Government.
I have the honour to acknowledge the receipt of a copy of Mr. Hooper's report giving the
results of his chemical analysis.
I have, &c.,
J. F. EVANS, M.B., Surgeon-Captain,
Chemical Examiner to Government of Bengal.
REPORT BY DR. GEORGE WATT, M.B., C.M., C.I.E.
231
fibre is a regular article of trade," and on his expectation that there may " possibly" be
" tracts of the central tableland of India similar to the Godavery district which could easily
grow the fibre-yielding forms of the plant." As regards the growth of hemp fibre
in the Godavery district, there should be no difficulty in finding out what the real fact is,
i.e., whether the " hemp" grown there is "sunn hemp," the product of a plant of the pea
family named Crotalaria juncea or ganja hemp. If the latter, then it might be worth
while to repeat, on a small scale, the Honourable Company's experiment of growing from
European seed.
5. The inner valleys of the Himalayas appear to me to be so handicapped by the heavy
cost of carriage of their produce to a seaport as to make it unlikely that hemp fibre grown
in them could compete successfully in the London market with fibre grown on the continent
of Europe. Moreover, when one considers the number of fibre-yielding plants which are
known to be indigenous to India, one rather doubts the expediency of selecting for encourage-
ment the cultivation of a species of which even the fibre-yielding race yields a narcotic so
potent as charas.
6. I regret that I am unable to explain the precise meaning of Dr. Watt in the sentence
alluded to by you and which ends as follows: " tracts of country where fibre is produced, or
where bhang only can be grown as in regions of ganja or charas production." From the context,
I gather that Dr. Watt suggests that excise regulations should be relaxed in regions where
the Cannabis plant produces fibre, even although it may also (as it in fact does) produce charas.
232
REPORT OF THE INDIAN HEMP DRUGS COMMISSION, 1893-94.
[APP.
MEMO. BY BRIGADE-SURGEON-LIEUTENANT-COLONEL G. KING, M.B.,
L.L.D., F.R.S., C.I.E., DIRECTOR OF BOTANICAL SURVEY OF INDIA.
1. The plant familiarly known as Indian hemp (Cannabis sativa, L.) is really wild in no
part of British India. But in all parts of India, and also in Upper Burma, plants of it may
be found growing without cultivation near villages and gardens where hemp is at present, or
has in former times been cultivated. In botanical phraseology, hemp is found in India, not
as an indigenous plant, but as an escape from cultivation.
2. By physical conditions, I understand soil and climate. As regards soil, the drug-
yielding variety is (as I am informed by Dr. Prain) grown on a large scale, and as a regular
crop, only in Rajshahve, in some parts of the tributary mehals of Orissa, and in some parts
of Central India. The soils of the tracts where it is so grown have not, so far as I am aware,
been analysed chemically; physically they are known to be friable and well drained. Soil,
however, does not appear to be a matter of much importance in the growth of the resin-
yielding hemp. For, with careful cultivation, it can, I understand, be grown as a garden
crop in any part of India. At elevations below 2,000 feet, the difficulty of cultivation is
very slight; from 2,000 to probably 9,000 feet there is no difficulty whatever. At higher
elevations than the latter it does not appear to be much grown. As regards climate there is
little to be said beyond that a period of continuous dry weather, extending over three or four
months of the year, is essential, and that temperatures like those prevailing in the middle
zone of elevation in the North-Western Himalaya appear to be the most favourable for the
development of the drug.
REPORT BY MR. H. M. LAWRIE, C.S.
241
STATEMENT I.
Showing the quantities of ganja sold at the ganja-shops in the Bhandara District during the
years 1891-92 to 1893-94.
GANJA SALES IN SEERS.
Name of tahsil.
Name of ganja-shop.
Remarks.
1891-92.
1892-93.
1893-94.
Bhandara
Ambagarh
45
33
50
These
ganja-shops
have many Po-
wars.
Sihora
34
32
50
These
ganja-shops
have many Po-
wars.
Bamhani
50
91
112
These
ganja-shops
have many Po-
wars.
Tirrora
Tirrora
70
72
88
These
ganja-shops
have many Po-
wars.
Gondia
73
99
84
These
ganja-shops
have many Po-
wars.
Rampaili
104
128
80
These
ganja-shops
have many Po-
wars.
Gond Mohali
70
97
100
These
ganja-shops
have many Po-
wars.
Fulchur
39
72
70
These
ganja-shops
have many Po-
wars.
Katangi
27
These
ganja-shops
have many Po-
wars.
Kati
71
124
133
These
ganja-shops
have many Po-
wars.
Kurhari
53
63
72
These
ganja-shops
have many Po-
wars.
Ekori
108
117
136
These
ganja-shops
have many Po-
wars.
Total
744
928
975
Bhandara
Tumsar
60
66
84
These
ganja-shops
have few Powars.
Tirrora
Beni
118
122
100
These
ganja-shops
have few Powars.
Katipur
52
79
78
These
ganja-shops
have few Powars.
Sarandi
26
33
31
These
ganja-shops
have few Powars.
Adasi
24
27
65
These
ganja-shops
have few Powars.
Murdara
83
113
183
These
ganja-shops
have few Powars.
Kamtha
53
69
87
These
ganja-shops
have few Powars.
Katori
58
50
65
These
ganja-shops
have few Powars.
Tirkheri
8
30
29
These
ganja-shops
have few Powars.
Sakhritola
67
49
72
These
ganja-shops
have few Powars.
Bijli
62
50
These
ganja-shops
have few Powars.
Amgaon
100
96
84
These
ganja-shops
have few Powars.
Lakhni
45
51
62
Total
694
847
990
Sakoli Tahsil
Bhandara
70
73
86
These
ganja-shops
bare no Powars.
The same contractor.
Bhandara
Adyal
34
31
37
These
ganja-shops
bare no Powars.
The same contractor.
Ponni
44
46
60
These
ganja-shops
bare no Powars.
The same contractor.
Jamb
25
24
34
These
ganja-shops
bare no Powars.
The same contractor.
Amdhalgaon
34
29
40
These
ganja-shops
bare no Powars.
The same contractor.
Mohali
11
43
76
These
ganja-shops
bare no Powars.
The same contractor.
Tirrora
Mundhri
55
46
36
These
ganja-shops
bare no Powars.
The same contractor.
Dewhara
46
8
18
These
ganja-shops
bare no Powars.
The same contractor.
Salekasa
41
These
ganja-shops
bare no Powars.
The same contractor.
Darekas
25
19
31
These
ganja-shops
bare no Powars.
The same contractor.
Sakoli Tahsil
Sakoli
28
26
27
These
ganja-shops
bare no Powars.
The same contractor.
Pohora
25
19
24
These
ganja-shops
bare no Powars.
The same contractor.
Kitari
16
26
20
These
ganja-shops
bare no Powars.
The same contractor.
Palandur
14
12
30
Dighori
7
3
Lakhandur
41
37
27
Sangerhi
21
22
31
Nawegaon
30
16
21
Palandur (Chichgarh)
14
24
Arjuni
40
43
53
Chichewara
23
32
31
Total
630
569
706
GRAND TOTAL
2,068
2,344
2,671
61
242
REPORT OF THE INDIAN HEMP DRUGS COMMISSION, 1893-94.
[APP.
STATEMENT II.
Showing sale of ganja in the whol e district.
Year.
Maunds.
Seers.
Remarks.
1879-80
25
37
1880-81
35
20
1881-82
38
7
1882-83
31
20
1883-84
40
3
1884-85
48
19
1885-86
65
23
1886-87
41
27
1887-88
35
16
1888-89
42
16
1889-90
46
5
1890-91
48
24
1891-92
54
15
1892-93
61
36
1893-94
66
35
Ten months' actuals,—55 maunds
29 seers.
BULLETIN BY MR. C. BENSON, M.R.A.C.
245
A month after planting the fields are hand-weeded, and about a fortnight later a plough is
run between the rows, and the plants are earthed up slightly. Two months after planting
out flowering begins, and then the removal of the male (here as elsewhere termed female) plants
begins. They are cut off near the roots and thrown away. This work goes on continuously
as long as male plants are found.
About February the plant begins to ripen and the harvest commences. It goes on till
the end of March. The plants are cut bodily with the sickle, and are laid out in the field,
where they grow for three days to dry in the sun. On the fourth day they are tied into small
bundles of about ten plants each, and then piled, head and tail, in the field. The
heaps are opened, and the bundles re-piled next day, the process being repeated over
several days. When the quantity to be dealt with is small and space allows, the bundles are
carried to the grower's house and there piled; but in all cases the crop is finally carried to the
house, and a month later the spikes are removed. Each spike is plucked off by hand and then
they are spread out on a hard floor in the open for one night in the dew to soften and become
pliable. In the morning the spikes are collected and stored in large gunny-bags, being packed
closely therein by a man treading them down into the bag. The produce is then ready for
sale, and may be kept for as much as two years.
In both localities it is stated that of late years the area planted with hemp has been reduc-
ed, the price offered for ganja having fallen with the restriction of the demand owing to the
introduction of the system of licensing retail vendors.
A few years ago, the crop was also grown to some extent in one village in the Palivendla
taluk, Cuddapah District, but its growth there has now been abandoned. It was then grown as
a garden crop, in rotation with garden korra (Setaria italica) or garden ragi (Eleusine coracana),
the plants being raised in seed-beds and then planted out. The method of manufacture adopt-
ed there appears to have resembled that still followed in Kistna.
62
NOTE BY MR. G. A. GRIERSON, C.I.E.
249
bhang plant from the Himalayas and gave it to mankind. Jōgis are well-known consumers
of bhang and ganja, and they are worshippers of Çiva.
In folk-songs, ganja or bhang (with or without opium) is the invariable drink of heroes
before performing any great feat. At the village of Bauri in Gaya there is a huge hollow
stone, which is said to be the bowl in which the famous hero Lōrik mixed his ganja. Lōrik
was a very valiant general, and is the hero of numerous folk-songs. The epic poem of Alhā
and Rūdal, of uncertain date, but undoubtedly based on very old materials (the heroes lived
in the twelfth century A.D.), contains numerous references to ganja as a drink of warriors.
For instance, the commencement of the canto dealing with Alhā's marriage, describes the
pestle and mortar with which the ganja was prepared, the amount of the intoxicating drink
prepared from it (it is called sabzī) and the amount of opium (an absurdly exaggerated quan
tity) given to each warrior in his court.
That the consumption of bhang is not considered disreputable among Rajputs may be
gathered from the fact that Ajabēs, who was court poet to the well-known Maharaja Bishwa-
nath Singh of Riwa, wrote a poem praising bhang and comparing siddhi to the "success"
which attends the worshipper of "Hari." Here there is an elaborate series of puns. The
word siddhi means literally 'success,' and hari means not only the god Hari, but also
bhang.
63
254
REPORT OF THE INDIAN HEMP DRUGS COMMISSION, 1893-94.
[APP.
and Mymensingh districts, and the worship soon became popular. In fact it spread like wild-
fire from one village to another among the ganja-smokers. Those that were not in the
habit of consuming ganja also followed their example.
The following circumstances assisted the spread of the worship:—
I.—The puja is open to all classes from Brahmans to Chandals and to the rich and the
poor. Caste does not stand in its way, and it may be performed almost every day and in
all seasons.
II.—The puja is a Manasik Puja (made in pursuance of a vow on the fulfilment of the
object desired). People have been led to believe that Trinath possesses the power of healing
the sick and fulfilling desires, and that those who neglect his worship meet with disgrace,
while those who observe it attain success in life. There are several stories in the Panchali
narrated in illustration of this statement. It is also popularly believed that in the house
where Trinath is worshipped cold, fever and headache do not appear.
III.—This is a cheap form of worship. The puja can be performed by even the poorest,
only three pice being required.
IV.—People of the lowest class can mix with those above them without distinction of
caste or creed on the occasion of these pujas.
V.—Ganja can be consumed by all in the name of a god, and the practice cannot be
looked down upon, because it is done under certain forms and religious ceremonies. It is also
popularly believed that those who mock the worshippers of Trinath shall be ruined and shall
be the victims of misfortune.
The worship prevails not only among the poor, but also among the well-to-do. The
latter often entertain their friends after the puja.
Women do not take any active part in the worship, but they often listen to the reading
of the Panchali.
The worship is more or less general in the following districts:—(1) Dacca, (2) Mymen-
singh, (3) Faridpur, (4) Backergunge, (5) Noakhali, (6) Tippera, (7) Chittagong, (8)
Bogra, (9) Sylhet, and (10) Pabna (Serajganj side).
The worship is on the decline. It is almost dying out among the educated bhodrolokes,
but among the masses it still exists.
I have ascertained the above facts from Dr. Chandra Sekhar Kali (brother of the origi-
nator, Ananda Chandra Kali) and many other respectable persons, and also from personal
enquiries in the Dacca, Chittagong and Rajshahi divisions.
256 REPORT OF THE INDIAN HEMP DRUGS COMMISSION, 1893-94. [APP.
In 1888-89 the number of seizures was abnormally high, the aggregate quantity of ganja
weighing as much as 17,753 tolas, and may be accounted for by the influx of a large body of
native (Punjabi and Madrassi) troops consequent on the annexation of Upper Burma.
The following statement shows the seizures of ganja during the last five years, also during
the first eight months of 1893-94:—
Year.
Quantity.
Tolas.
1888-89
17,7533/4
1889-90
626
1890-91
1,218
1891-92
2,430
1892-93
5,856
1893-94 (from 1st April to 30th November)
30,353
ANALYSIS OF CASES.
263
Serial
No.
Province.
Name of witness.
Number
of
witness.
Name of criminal.
Nature of the crime.
Reason for attributing the crime to
hemp drugs.
18
North-Western
Provinces.
Mr. R. Wall
233 Not given
2. A sepoy shot Lieutenant
Coode at Benares in 1883.
"I cannot say whether it was
a hemp case."
19 Punjab
Shekh Riaz Hossain
67
Not given
A fakir murdered a Tahsil
chaprasi in Muzuffergarh.
He "was a constant consumer
of bhang and did nothing
else."
20
Do.
Mr. Robinson
16
Do.
A man nearly killed a banya
with whom he had had deal-
ings.
He was "under the influence
of some drug."
21
Do.
Karm Elahi
44
Do.
A Sikh in Rawal Pindi throttled
his nephew 8 or 9 months
old.
He was "under
excite-
ment," and apparently insane,
not under the influence of
bhang at the time.
22
Do.
Colonel Hutchinson
4
Do.
A Gurkha belonging to the
witness' then regiment ran
amok. It was in the sixties.
There was jealousy in the
case.
No reason given. "It is too
long ago to recollect details."
23
Do.
Mr. Warburton
30
Do.
A police sergeant about 1874
became violent from the use
of bhang and charas. Had
he got a sword there would
have been injury.
Witness' recollection. "He
had been brooding over
being passed over for pro-
motion."
24 Central Prov-
inces.
Hosen Khan
79 Not given
The witness' brother-in-law
nearly killed his wife under
the influence of ganja.
No special reason given.
25
Do.
Adhar Singh Gour
69
Do.
A confirmed smoker killed his
own child.
The Sessions Court acquitted
the man on account of in-
sanity due to mental trouble.
26
Do.
Taradas Banerjee
70
Do.
A Sanyasi killed a Kapalik in
the temple over 20 years
ago.
He was a ganja-smoker, but
witness cannot say he in-
dulged that day.
27
Do.
Brijmohan Patnaik
24 Paltu
Tried to kill a woman under
the influence of ganja-
No reason given. Witness does
not know about provocation.
28
Do.
Dinanath, Rai Baha-
dur.
32 Not given
A Gond killed his wife be-
cause she would not give
him water.
The man said he had done
the deed under the effect of
ganja.
29
Do.
Mr. Naylor
30
Do.
A Banjara killed his wife and
two members of the tanda
18 years ago.
The people of the tanda said
so.
30
Do.
Do.
30 Do.
2. A zamindar 15 years ago
killed his brother by whose
death he succeeded to the
estate.
His friends said so.
31
Do.
Prem Singh
57
Do.
"I have seen a boy, not used
to ganja, on becoming in-
toxicated with it, kill his
father."
Witness was present appa-
rently.
32
Do.
Mahomed Taki
33 Guppu Singh
Guppu wounded with a sword
one of several constables who
were drinking bhang with
him 20 years ago.
The story is that he was drink-
ing bhang at the time.
33
Do.
Do.
33 Not given
2. A Nat killed his wife 25
years ago.
The story is that he was drink-
ing bhang at the time. "He
subsequently became a luna-
tic and was sent to Jubbul-
pore."
34
Do.
Mr. Pasley
34
Do.
One Sadhu killed another
No reason given.
35 Madras
Mr. Simpson
67 Not given
A native officer shot his Suba-
dar Major from jealousy
about 1886.
Witness gives up this case.
(N.B.—This case is quoted
by witnesses Nos. 81
and 82.)
264
REPORT OF THE INDIAN HEMP DRUGS COMMISSION, 1893-94.
[APP.
Serial
No.
Province.
Name of witness.
Number
of
witness.
Name of criminal.
Nature of the crime.
Reason for ascribing the crime to
hemp drugs.
36 Madras
Mr. Simpson (con-
tinued).
67
Not given
2. A barber cut the throat of
his first wife and his own
that they might die together,
about 1883.
2. Witness is doubtful about
this case.
37
Do.
Do.
Do.
3. A boy in mischief threw a
stone over a purdah and
killed a woman sleeping on
the other side.
3. This is very doubtful; the
people present said "the lad
had thrown the stone in play."
38
Do.
Iyaswamy Pillay
109
Do.
A consumer was quarrelsome
and used to attack his rela-
tives and beat them.
The matter never came to
Court. The man took liquor
also.
39
Do.
Dr. Lancaster
90 Do.
A sepoy in hospital at Nellore
stabbed the orderly. He
was "in a state of doubtful
sanity" (vide Dr. Pem-
berton's evidence).
He used ganja.
He had
ultimately to be sent to the
asylum.
40
Do.
Dr. Pemberton
98
Do.
A sepoy in Burma in 1881
ran amok. This witness
also speaks to the Nellore
case (No. 39 supra).
The witness had the man un-
der observation.
41
Do.
Dr. Chatterjee
88
Do.
A sepoy ran amok in 1887 in
Burma.
The discrepancies between Dr.
Chatterjee's
written
and
oral examination show his
recollection to be defective.
42
Do.
Vasadeo Rajamani
128
Do.
A consumer tried to stab an-
other man 23 years ago.
The man was a consumer.
43
Do.
Mahomed Murtaza
82
Do.
A sepoy shot a Jamadar with-
out reason.
The men of his regiment said
he was a great smoker.
44
Do.
B. C. Chatterjee
167
Do.
A man in Ganjam committed
a murder.
"He was an immoderate smoker
of ganja."
45
Do.
Revd. Mr. Higgins
150
Do.
A Brabman beat his child and
wept when he became sober.
The man made this excuse.
46
Do.
Very Rev. A. Chel-
vum.
161
Do.
A sepoy was anxious 42 year
ago to do violence but could
not control his limbs.
No reason given. Witness was
then twelve years old.
47
Do.
Mr. Merriman
28
Do.
A pensioned sepoy murdered
his daughter-in-law and her
brother and sister.
The man was a consumer.
48 Bombay
Mr. Kennedy
54 Not given
A Biluch sepoy ran amok and
shot some people.
Witness rather indicates that
this was premeditated.
49
Do.
Mr. Woodward
11
Do.
A man attacked and was
shot by Mr. Woodward, Col.
lector of Nasik.
"It was commonly reported."
50
Do.
V. R. Katti
116
Do.
A Lingayat tender killed his
brother by night.
He was a consumer.
51
Sind
Puribhdas Shewakram
Advani.
29 Not given
A sepoy at Hyderabad ran
amok several years ago.
"It was believed." Mr. Giles
(Witness 2) also quotes this
case doubtfully.
52 Do.
Rahmatala Khan
9
Do.
A drinker of bhang committed
a murder in a scuffle about
plucking berries.
The man was a consumer.
53 Do.
Mahomed Murid
11 Kowra Khokar
Kowra Khokar killed a com-
panion at night.
"They were always quarrelling
about opium and bhang."
54 Burma
Mr. Mayne
23
Not given
A sepoy shot a Subadar-
Major at Sagaing in 1888.
He was a consumer.
55
Do
Do.
Do.
Do.
2. Another sepoy attempted
to murder a Burmese girl.
Ditto.
56 Borar
G. V. Khot
30
Do.
A man ran amok in Mhow
No reason given.
I P / 2 5 / P Z . 4
I P
BULLETIN OF THE
PASTEUR I NSTI TUTE
OF SOUTHERN I NDI A, COONOOR.
NO. 3. 1910.
P U B L I S H E D U N D E R T H E A U T H O R I T Y O F T H E C E N T R A L
COMMI T T E E OF T HE A S S OCI A T I ON.
MADRAS.
P R I N T E D B Y T H E S U P E R I N T E N D E N T , G O V E R N M E N T P R E S S .
1911.
STAFF.
D i r e c t o r :
J. W. CORNWALL, M.A., M.D., D.P.H., Major, I.M.S.
Assistant Director :
A. G. MCKENDRICK, M.B., CH.B., Captain, I.M.S.
Assistant Surgeon : M. KESAVA PAI, M.D.|
Sub-Assistant Surgeon : S. RAMASAMY AIYAR.
TABLE OF CONTENTS.
SECTION I.—NOTES ON RABIES.
PAGE
1. Deviation of complement by fixed virus brain extract. By J. W. Cornwall and
M. Kesava Pai
...
...
. . .
...
. . .
. . .
. . .
. . .
. . .
1
2. Reduction of Methylene Blue by fixed virus. By J. W. Cornwall
...
...
...
2
3. Cutaneous reaction in antirabic inoculations. By J. W. Cornwall
...
...
...
3
SECTION II.—HÆMOLYTIC DATA.
1. Some factors which influence the lysis of erythrocytes by hypotonicity.
By J. W. Cornwall and M. Kesava Pai
...
. . .
. . .
. . .
. . .
. . .
4
2. Some facts relating to amboceptors. By J. W. Cornwall and M. Kesava Pai
. . .
7
3. On the ponderability of amboceptor. By J. W. Cornwall
...
. . . ...
. . .
18
4. Isolysins. By J. W. Cornwall ...
...
...
...
...
...
. . .
...
19
5. Some facts relating to complement. By J. W. Cornwall and M. Kesava Pai
. . .
20
6. The complementing power of homogenous serums. By J. W. Cornwall and
M. Kesava Pai
. . .
...
...
...
...
...
...
...
...
25
7. Deviation of complement. By J. W. Cornwall
...
...
...
...
...
28
8. The latent antilytic property of normal serum. By J. W. Cornwall ...
...
...
31
9. Various factors which influence specific lysis. By J. W. Cornwall and M. Kesava
Pai
...
. . .
. . .
. . .
. . .
. . .
. . .
. . .
. . .
...
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33
10. On the effect of the presence of a heated serum on a hæmolytic system.
By J. W. Cornwall and M. Kesava Pai
...
...
. . .
. . .
...
...
33
11. Anaphylaxis. By J. W. Cornwall
. . .
. . .
. . .
. . .
. . .
. . .
. . .
37
b
BULLETIN
OF THE
PASTEUR INSTITUTE
OF
SOUTHERN INDIA.
SECTION I.
NOTES ON RABIES.
I. Devi ati on of Compl ement by Fi xed Vi rus Brai n Extract.
Numerous authors have recorded their failures to obtain a specific deviation of
complement reaction with antirabic serum and the blood, brain or other tissue of
an animal suffering from rabies, and our own experiments also (Bulletin 1, 1908,
page 24) in this direction have been, until recently, negative. By using extracts of
brains we have now obtained some positive results, though the reaction could not
be demonstrated at will, even when the conditions which had been successful were
reproduced as nearly as possible.
The extracts were prepared by taking equal weights of fixed virus rabbit brain
and of normal rabbit brain, pounding them in a mortar with 0 . 85 per cent. NaCl,
and leaving the resulting emulsions (1 in 10) in the ice box for 24 hours. The
solid matter was then deposited by centrifugation and the faintly opalescent fluids
used.
TABLE No. 1.
1
2
3
4
5
6
1.0
1.0
1.0
0.42
0 . 4 2
0.42
Extract, fixed virus brain .
0.75
1.2
Extract, normal brain
0.75
1 . 2
S alt, 0.85 per cent.
0.75
0.13
0.13
1.33
80 per cent. sensitized sheep RBCs
0.25
0.25
0 . 2 5
0 . 2 5
0 . 8 5
0 . 2 5
Lysis.
Partial. Complete. Complete. Slight.
Partial.
Nearly
complete.
The extracts were incubated with the antirabic serum for 1 hours before the
/sensitized RBCs were added. Column 1 shows less lysis than column2 ,
a n d
col umn 4 t han col umn 5• The amount of compl ement avai l abl e i n col umn2 w a s
To face page 4
CHART No.
Position on the salt percentage scale of the
mean range of lysis of the R B Cs. of
eight species.
Position on the salt percentage scale of the mean range
of lysis of erythrocytes from eight different species.
P e r c e n t a g e
N a C l
S . O . R e g : N o . 1 2 6 8
1911 Copies—3 +208