12 REPORT OF THE IMPERIAL INSTITUTE OF VETERINARY RESEARCH,
days, respectively, after the last injection. Careful consideration of all
the available facts leads to the conclusion that these three cases almost
certainly represent re-infections and not relapses. One of the three-
animals was re-treated by the same method and is reported to be cured.
If the three cases of recurrence of the disease are included, however,
then the trial resulted in cure in 48 cut of 51 animals, or 94.11 per
cent.
Eighteen couple of the Delhi Hunt Hounds were accommodated at
Muktesar from the 18th March to the 18th October, 1928. Ten of the
hounds had been diagnosed as affected with surra during the previous
winter and had received treatment by injections of" Bayer 20.5", as
recorded in the previous annual report of this Institute. The hounds
were kept under close observation during the whole period of their stay
at Muktesar and no recurrence of the disease took place. The treat-
ment devised by Dr. Edwards thus proved adequate to cure the disease
in the hounds.
(c) Piroplasmosis. An investigation of much importance was con-
ducted by Dr. Edwards upon acute Theileriasis affecting Holstein stud
bulls imported by the Military Department into India from South
Africa. Nineteen bulls arrived at Bombay on the 27th October, 1928,
and twelve of them, which were distributed to the Northern Circle
Dairy Farms, became affected with the disease during the period the
11th to the 24th of Novemver 1928. These bulls were probably sub-
jected to heavy tick infestation very shortly after their arrival
in India, and also during transit before distribution, resulting in a
sudden and massive infection with Theileria parasites. Six of the
remaining bulls were drafted to the Southern Circle Dairy Farms and
did not become affected with the disease during the period cited, al-
though it has since been confirmed that one of these bulls died of the
same disease shortly afterwards. The disease in the 12 bulls resulted
in three deaths; one bull was very severely affected but recovered;
while eight were markedly affected and two of them suffered from a
relapse some five months later.
The opportunity afforded by this investigation enabled Dr. Edwards
to place on record a complete summary of the disease. The symptoms
described are very briefly as follows. The disease starts with a sudden-
ly occurring and prolonged fever, during which the temperature remains
constantly very high (105° to 107°F.). No other symptoms are exhi-
bited, however, until the parasites (T. mutans) make their appearance
in the blood. The temperature then fluctuates, falling and rising ir-
regularly and the parasites increase rapidly in number to a maximum
of about 70 per cent. of invaded red blood corpuscles. From 1 to 10
parasites were seen in single corpuscles. Other symptoms also begin
to be exhibited, the most striking and charateristic being profuse lacri-
mation, and a watery discharge from the nostrils. Depraved appetite
and a harsh "staring" coat are observed. Loss of condition occurs in
spite of the fact that the animal may feed quite well.
Symptoms of marked respiratory disease, with particularly painful
respiration, resembling "pleuritic pain", were noticed in one fatal case.
These symptoms may be attributed to extreme anæmia exhibited, and