33
finding corresponds with the quantities of nerve substance, containing rabbit R.B.Cs., injected
into the respective goats. The cyllin preserved virus was evidently unsuitable for the purpose
of causing the formation of hemolytic amboceptors.
Fresh human serum dissolves rabbit R.B.Cs. and its normal amboceptor, unlike that of
goats, can be well complemented by fresh rabbit serum. The same experiment is on this account
more difficult to perform with the serums of the patients, added to which the quantity of nerve
substance injected into them and likewise of rabbit R.B.Cs. is very much less than the quantity
injected into the goats. One would not, therefore, expect a very marked formation of rabbit
hæmolysin in the serum of the patients, though there might be enough to measure—
Patient 77 received in 18 days 1/17,000 of his weight of fixed virus rabbit cord preserved for
some days in glycerine.
Before treatment it required
—
•5 cc. of hi s heat ed serum t o gi ve compl et e lysis.
And after treatment—
•5 cc. of his heated serum to give complete lysis, using fresh rabbit serum as complement and
a
5 per cent. suspension of rabbit R.B.Cs.
No artificial amboceptor was demonstrated in this case.
Patient 91 received in 14 days 1/31,000of his weight of fixed virus rabbit cord preserved for
some days in glycerine.
Before treatment it required—
•5 cc. of his heated serum to give complete lysis.
And after treatment
—
• 3 5 c c : o f h i s h e a t e d s e r u m t o g i v e c o mp l e t e l y s i s .
So in this case some artificial amboceptor was formed.
Patient 141 received in 14 days 1/15,000 of his weight of fixed virus rabbit cord pre
served for
some days in glycerine.
Before treatment it required—
•3 cc. of his heated serum to give complete lysis.
On the sixth day of treatment
•3 cc. of hi s heated serum to gi ve compl ete l ysi s.
And after
treatment—
• 2 c c . o f h i s h e a t e d s e r u m t o g i v e c o mp l e t e l y s i s .
So again in this case there was some artificial amboceptor formed.
As the differences were evidently too small to have any practical value no further experiments
were made.
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(3) Remlinger. C.R. Soc. Biol. XXXVII, 1905.
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(6) Krasmitski. Annales de l'Inst. Past., June 25, 1902.
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