Goat C —Weight 24 kilos—First series of . inoculations.—Received 6 cc. 1 in.10 fresh fixed
virus daily, subcutaneously, for 10 days, May 31st to June 9th, 1907. Six grams brain sub- .
stance, 1/4000 of its body weight.
On 4th day of treatment 200 serum did not destroy 1 virus.
:, 8th
„
100 „
,,
,,
1 53
200
„
did „ 1
/7
75
55
7/
3 days after'the end 25 „ did not
„
1 11
50
did
1
11
1)
75
I)
7/
10 days
,,
25 ,, did not1 35
.
50
did.
1
5)
7 )
77
,,
1 5 d a y s
5 7 1
7 5
, ,
1 , ,
21 „
,,
5 „ did. not „
1 „
35
,,
5 7/
,,
77
13
44 10
,,
,,
1 I,
59 „
20 „
13
71
1 „
The goat was bled to 200 cc. on the 59th day and the serum was found to have retained its
power of 20 to 1 after 30 days' storage in the ice box. The goat then had two healthy kids
which it suckled.
One hundred and fifty-two days after the end of its treatment 80 of its fresh serum did
not destroy 1 virus, nor did the serum of its kids when they were 65 days old destroy
virus in the proportion of 100 to 1.
.
Second series or inoculations.—It then received 5 cc. of 1 in 10 fresh fixed virus daily for 10
days, November 17th-26th, 1907, 5
15 days later 40 serum did not destroy 1 virus.
80
did
1
Third series of inoculations.-36 grammes fresh fixed virus brain between December 30th,
1907 and January 25th, 1908.
20 days later 10 serum destroyed 1 virus.
Fourth series of inoculations.-23. 4 grammes fresh fixed virus brain letween February 1.6th
-and February 28th, 1908.
.
20 days later 20 serum did not destroy 1 virus.
51
71
10
1 „
.
Fifth series of inoculations —18 grammes fresh fixed virus brain between May 4th and 14th,
1908.
•
25 days later 80 serum did not destroy 1 virus.
Sixth series of inoculations.-12 grammes fresh first passage street virus rabbit brains between
August 12th and 23rd, 1908.
17 days later 10 serum destroyed 1 virus.
Thus Goat C received altogether 88 . 4 grammes fresh fixed virus during eight months and the
antirabic power of its serum varied considerably from time to time becoming less after the fourth
and fifth series of inoculations and improving again after a course of street virus.
Goat D.—Received 10 cc. of 1 in 100 fixed virus emulsion, filtered through paper and
warmed to 37° C, daily for 4 days intravenously, less than 4 gramme brain substance.
6 days after end 50 serum did not destroy 1 virus.
It then received 83 cc. of 1 in 100 fixed virus emulsion, filtered through paper and warmed
to 37° C, intravenously in 5 days, less than 1 . 53 grammes brain substance.
3 days after the end 50 serum did not destroy 1 virus.
The intravenous method has been extolled as a rapid and effectual way of producing an
antirabic serum. In this animal it certainly did not succeed. The goat had a young one 2
months later. Two months after that event it received 2 cc. of tin 10 fixed virus subcutaneously
for 10 days, 2 grammes brain substance.
15 days after the end 80 serum did not destroy 1 virus.
•
Goat D then received subcutaneously 23 . 4 grammes fresh fixed virus between February
6th to 28th, 1908,
21 days later 20 serum did not destroy 1 virus.
.
41
4 0 1
11
15
-
75
51 20
1
Between May 4th and 14th, 1908, it received 18 grams fixed virus subcutaneously,
25 days later 80 serum did not destroy 1 virus.