10
Goat 3.—Received 1 gramme fixed virus in cyllin subcutaneously in 11 days.
26 days afterwards 10 serum did not destroy 1 virus.
50
did
1
133 100
did not
1
' Four months later this goat received 1 . 66 grammes fixed virus in eyllin in 10 days.
15 days afterwards 80 serum Mid not destroy 1 virus.
Between February 16th---28th, 1908, Goat 3 received 11 .7 grammes fixed virus in cyllin.
21 days later 80 serum did not destroy 1 virus.
41
)
40 ,,
did
„
1 „
51
80
/
did not ,, 1
Between May 4th-14th, 1908, 9 grammes fixed virus in cyllin were injected.
25 days later 80 serum did not destroy 1 virus.
Rabbit 87.—Received '2 .72 grammes of fixed virus in cyllin subcutaneously in 15 days, 1/560
of its body weight,
21 days afterward
25 serum did destory 1 virus
54 10
did not
1
25
did
1
143
25
1
208
50
did not
1
Rabbit 88.—Received 2 . 4 grammes of fixed virus in cyllin subcutaneously in 12 days, 1/470 of
its body weight,
21 days afterwards 50 serum destroyed 1 virus.
46
25
1
63
10 serum did not destroy 1 virus (incubation 12 days).
25
did
1 virus
143 10
did not
1virus(incubation 11 days).
25
did
1 virus
215
50 di d not 1vi rus.
Fermi (33) has recently reported good results from the use of a 1 in 10 emulsion of fresh
fixed virus containing 1 per cent. phenol, which kept good for at least four months. He saved
100 per cent. of rats from previous and subsequent subcutaneous inoculation with street virus
by 30 cc. of the above mixture subcutaneously in 15 days. The average weight of his rats was
apparently about 100 grammes, so each animal required 3 grammes of brain substance or 3 per
cent. of its weight to save it.
Applying similar proportions to human beings, a man of 150 lb. (68 kilos) would require
-4½- lb. (2 kilos) of brain substance to be injected in the form of an emulsion within 15 to 20 days,
which would amount to a little more than 100 cc. daily in 20 days. The treatment would not
be popular. Bullocks would also have to be substituted for rabbits as the source of the virus.
The ideal treatment, which must be safe, certain, brief, applicable any where by any
physician and at the same time one whose effects can be measured and registered, has still to be
discovered.
.5. THE DEGREE OF INFECTIVITY OF VARIOUS PARTS OF THE CENTRAL
NERVOUS SYSTEM IN RABIES IN RELATION TO THE TREATMENT
OF PATIENTS.
BY
J. W. CORNWALL AND M. KESAVA PAL
As might be expected one finds considerable disagreement in the results recorded by various
workers of inoculations made with the object of ascertaining whether any one part of the brain and
spinal cord is of greater infectivity than the rest.
These differences probably depend as much on the diversity of the strains of virus used as
on the way in which the experiments were conducted, though it is exceedingly unlikely that a list
could ever be drawn up which would prove invariably correct, for the disease appears at times to
attack some one part more severely than another. The importance of fairly definite knowledge of
the degree of infectivity of different parts is realised when the question of the treatment of
patients comes under consideration.
Assuming for the moment that the cervical cord contains ten times as many organisms as
the lumbar cord, it is conceivable, to take an extreme case, that, unless the place of origin of each
fragment of cord in stock is carefully labelled, and heed is taken to give all patients a similar dose