30
This action of chloroform vapour on the organism of rabies indicates that it is less resistant
to the drug than the organism of vaccinia, a point of some interest, if not of importance,
considering that the two organisms appear to be related and are classified together in the order
-of Chlamydozoa by Prowazek (35).
11. THE INFECTIVITY OF THE PERIPHERAL NERVES IN RABIES.
BY
J. W. CORNWALL AND M. KESAVA PAL
TABLE No. 17 showing result of subdural inoculation of emulsions of rarious nerves derived
from rabid animals in rabbits.
Nerve used,
.
Animal
providing
virus.
Incubation
period.
Days.
Animal
providing
virus.
Incubation
period.
Days.
Animal
providing
virus.
Incubation
period.
Days.
Animal
providing
virus.
Incubation
period.
Days,
Right sciatic upper half...
Fi xed vi r us pas s age r abbi t
11
R a b b i t w h i c h h a d d i e d f r o m a s i n g l e s u b c u t a n e o u s
i n o c u l a t i o n w i t h f i x e d v i r u s .
9
R a b b i t w h i c h h a d d i e d f r o m a s i n g l e s u b c u t a n e o u s i n o c u l a t i o n w i t h f i r s t p a s s a g e s t r e e t v i r u s f r o m a n a t u r a l l y i n f e c t e d d o g .
N a t u r a l l y i n f e c t e d s t r e e t v i r u s d o g .
l
Right sciatic lower half... ...
10
8
15
Survived.
Left sciatic upper half ...
Died other-
wise 5th day.
—
8
Left sciatic lower half ...
9
Survived.
••.
•••
Right, tibial
...
...
10
Survived.
1 8
Left tibial
..,
11
Survived.
21
Right median ...
.„
Died other-
WiSe 31st (lay.
Survived,
19
--
...
Left median
...
...
10
10
Survived.
Right vagus
..,
..•
7
"
--
Left vague
..
...
Survived.
S
24
Survived.
It would appear from this experiment that the infective agent is widely scattered throughout
the peripheral nervous system, but that it is not present in a quantity sufficient to be uniformly
fatal to rabbits in subclural inoculation of an emulsion of a portion of the nerve. Fixed virus
nerves and street virus nerves tend to preserve their respective characteristics as regards the
length of incubation, though this period is somewhat prolonged in the case of fixed virus. Un-
fortunately no examination for Negri bodies was made.
12. A METHOD OF ESTIMATING THE PERCENTAGE OF
CHLORIDES IN SERUM.
BY
J. W. CORNWALL AND M. KESAVA PAI
Not being entirely satisfied with the existing methods of estimating the quantity of chlorides
in serum as regards either their accuracy or applicability, we devised the following procedure,
which we have reason to believe meets the ease, in that it combines with simplicity of manipulation
:as high a degree of accuracy as can be obtained when the end point of a reaction in a turbid
fluid is indicated by a change of colour to be judged by the eye. All methods of estimating
chlorides depend for their end points on a colour reaction and therefore give too high a figure,
and this also applies when a large bulk of serum . is evaporated to dryness and the salts are
extracted from the residue after incineration. The figure obtained in this way after incineration
approaches the actual as nearly as analytical chemistry will allow, and in- the hands of different
observers gives a range of 0·45 per cent.—0.60 per cent, NaC1 as the normal content of the serum.
Our method necessitates the employment of only 100 c.mm. of serum from each individual on
each occasion that an observation is made. Blood is collected from a finger puncture or in a
small syringe from a vein and allowed to clot in a sealed pipette. It is convenient to wait until
8—12 specimens of serum have been collected before proceeding with the estimations. The
.serum used. must be quite clear, for the least trace of lysis in it vitiates the readings. Two test-