28
TABLE NO. 15.
T ype of leucocyte.
P e r c e n t a g e b e f o r e s t a r v a t i o n .
P e r c e n t a g e a f t e r o n e d a y ' s s t a r v a t i o n .
P e r c e n t a g e a f t e r t w o d a y ' s s t a r v a t i o n .
P e r c e n t a g e a f t e r t h r e e d a y ' s s t a r v a t i o n .
P e r c e n t a g e a f t e r f o u r d a y ' s s t a r v a t i o n
P e r c e n t a g e a f t e r f i v e d a y ' s s t a r v a t i o n .
P e r c e n t a g e a f t e r s i x d a y ' s s t a r v a t i o n .
P e r c e n t a g e a f t e r s e v e n d a y ' s s t a r v a t i o n .
P e r c e n t a g e a f t e r e i g h t d a y ' s s t a r v a t i o n
Small mononuclears ...
...
68. 7
64
66.5
70
74'5
66
69
65
65
Polymorphonuclears ...
...
22 . 5
30
20
25 .
18
24
24
27
24
Eosinophiles ...
...
3.5
3
2.5
1
'8
2
0
1
0
Large inononuclears ...
..
2
2
2'8
2
2'5 I
2
4
5
4
Transitionals ...
...
...
2.8
1
2
2
3.7
6
2.5
2
7
Basophilo polymorphonuclears.
*5
0
2
0
*5
-
0
.5
0
0
Weight of guinea-pig ..,
...,
580
grammes.
•..
480
grammes.
...
460
grammes.
.,,
400
grammes.
.,,
360
grammes.
9. THE SUSCEPTIBILITY OF GUINEA-PIGS TO RABIES AND THE
RESULT OF ATTEMPTS AT rMMUNISINGTHEM.
BY
J. W. CORNWALL AND M. KESAVA. PAl.
A.—Fixed Virus.
Fresh fixed virus brain when inoculated subcutaneously into guinea-pigs in the abdomen
in a single dose in any quantity between 1/400 and 1/1,000,000 of the body weight gives rise to
rabies in 46.5 per cent. The disease develops within 21 days as a general rule.
In the case of rabbits single doses of fresh fixed virus brain between 1/400 and1/4,000
body weight cause rabies in 33 per cent.
Guinea-pigs which received subcutaneous inoculations in the abdomen on 14 successive days
of fixed virus cord preserved in glycerine or of fresh fixed virus brain to a total of either 1
/10,000
or 1/1,000 of the body weight developed rabies to the extent of 10.3 per cent.
B.—Street Virus.
Single subcutaneous doses in the abdomen of first passage street virus brain cause rabies in.
guinea-pigs to the extent of 92.4 per cent.
The disease develops within 25 days as a general rule. Guinea-pigs which received sub-
cutaneous inoculations in the abdomen of first passage street virus cord preserved in glycerine to
a total of either 1/10,000 or 1/1,000 of the body weight developed rabies to the extent of 70 per cent.
It .is therefore clear that it is not possible to immunise guinea-pigs with safety against rabies
by repeated subcutaneous injections of either fresh or glycerinated fixed. or street virus. Better
results are obtained when the injections are confined to the abdominal region than when they are
made indiscriminately in any part of the body. Daily inoculations in the abdomen also give
better results than equivalent weights administered in four large doses at intervals of four days.
The immunity attained by the animals referred to above was subsequently tested in various
ways and an analysis of the results brings the following facts to light :
—
(1) That immunity to subsequent subcutaneous inoculations with fixed virus is complete.
(2) That guinea-pigs which have received either single or multiple subcutaneous inocula-
tions of fixed virus are sometimes immune to subdural inoculation with either fixed virus or
street virus, and sometimes succumb.
(3) That guinea-pigs which have received either single or multiple subcutaneous inoculations
of fixed virus are generally immune to street virus, either subcutaneously or intraocularly, but
not always,