32
This table does not exhibit the difference between rice eaters and those who live on a mixed
diet which McCay showed to be the case in Bengal, but it may be that the conditions of life
on these hills 0,000 feet) tend to level up such differences. We thought it possible that some
concentration of the salts of the serum might occur in persons arriving newly from the plains
owing to loss of water from the tissues in the colder climate, so this point was tested before
proceeding with the examination of the serums. of our patients. Similarly the normal daily
variations in the serum of residents in the hills was ascertained :--
Max, Min. Mean.
Normal variation over a period of days of residents in
hills
· 70
· 6 0
· 6 4 6
Variation of new arrivals from plains over a period of
days
· 67
· 6 3
·643
It was evident that a large range might be expected from day to day in normal individuals
and that no increase of chlorides is to be anticipated in new arrivals.
TABLE No. 19 showing the influence of the antirabic treatment on the chloride content of the
serum of 20 patients.
Maximum.
Minimum.
M e a n .
Before inoculation...
...
·65
·60
·63
After 6th inoculation
...
·68
·41
·637
After 14th and last inoculation ..
·68
· 5 9
·641
It is clear from these estimations that no marked rise or fall in the chloride content of the
serum of patients results from the antirabic treatment in use at Coonoor and that the variations
in quantity observed do not exceed the limits which are met with in untreated persons.
13. THE FORMATION OF A. HEMOLYTIC AMBOCEPTOR IN THE BLOOD
OF PATIENTS AS A RESULT OF ANTIRABIC INOCULATIONS'.
BY
J. W. CORNWALL AND M. KESAVA PAI.
These experiments were performed with the idea of finding some method which might enable
a judgment to be formed of the progress made by the patients under treatment towards immunity,
supposing that any relation could be made out between the formation of a rabies antibody
and the formation of a rabbit blood corpuscle lysin. The presence of the lysin is without doubt
due to the injection into the patients of a certain quantity of rabbit R.B.Cs. with the nerve
substance.
Trial was first made with the serums of three goats which were being used for the preparation
of an antirabic serum
Goat C had received over a period of 12 months 88.4 grammes of fresh fixed virus rabbit
brain.
Goat D-63·3 grammes in 12 months of fresh fixed virus rabbit brain.
Goat 3-29·3 grammes in 8 months of fixed virus rabbit brain preserved in cyllin.
Normal goat serum dissolves rabbit R.B.Cs. but when previously heated to 55° C
for-30 minutes, its normal amboceptor is either not at all or only slightly complemented by fresh
rabbit serum, whereas the artificial amboceptor induced by the subcutaneous injection of rabbit
R.B.Cs. is well complemented by fresh rabbit serum.
On the occasion of this experiment ·25 cc. of the fresh serums of a normal goat, goat C, goat
D and goat 3 all dissolved 1 cc, of 5 per cent. rabbit R.B.Cs. completely, but it required--
*25 cc. Goat C serum (heated) to give complete lysis.
50 cc. Goat D
"
"
"
"
1·0 cc Goat 3
"
"
partial
"
1·0 cc_ Normal goat serum (heated) to give partial lysis.
When complemented with fresh rabbit serum, goat C serum, therefore, contained the largest
amount of artificial hæmolytic amboceptor, goat D the next largest and goat 3 the least. In
fact goat 3 serum contained no more amboceptor than the serum of the normal goat. This