TABLE III—cont.
Serial
num
-ber.
Case
num-
ber.
Medical Officer.
Disease.
Vaccine.
Result.
12
92 The Civil Surgeon, Tellicherry . . . Multiple diabetic
abscesses.
Staphylococcus
aureus.
No effect.
13
93 The Third Surgeon, General Hospital,
Madras.
Psoas abscess . . .
Do.
Not known.
14
106 The Surgeon, First District, Madras . . . Sinus groin . . .
Do.
Cured.
15
110 The Third Surgeon, General Hospital,
Madras.
Abscess . . .
Do.
Not known.
16
111 Dr. U. Rama Rao, Madras . . . Carbuncle . . .
Do.
No effect.
17
117 The District Medical and Sanitary
Officer, Cocanada.
Do. . . .
Do.
Not used.
18
123 Dr. P. Lakshmanan, Quilon . . . Diabetic carbuncle . . .
Do.
Cured.
19
124
Do. . . Abscess . . .
Do.
Do.
20
137 Miss Macphail, Conjeeveram . . . Carbuncle . . . Streptococcus . . .
Cured, but the
patient died
later from
complications.
21
138 The District Medical and Sanitary
Officer, Cuddalore.
Abscess . . . Staphylococcus
aureus.
Not known.
22
152 Mr. S. Muniswami, Trichinopoly . . . Carbuncle . . .
Do.
Not used.
23
157 The District Medical and Sanitary
Officer, Vellore.
Do. . . .
Do.
Cured.
24
159 The Superintendent, Lunatic Asylum,
Madras.
Abscess . . .
Do.
Not used.
25
163 Dr. L. A. Ravi Varma, Trivandrum . . . Diabetic abscess
Streptococcus . . .
Not known.
26
191 Assistant Surgeon B. Keshava Rao,
Chowghat.
Carbuncle . . . Staphylococcus
aureus.
Do.
that this disease is of such a chronic and persistent nature the
results obtained may be considered to be on the whole good. The acne bacillus was cultivat-
- e d . a n æ r o b i c a l l y b y t h e f o l l o w i n g m e t h o d . A b o u t 1 0 t o 1 5 c . c . o f o r d i n a r y b o u i l l o n w e r e
boiled in a sterile test tube to drive off the air dissolved in the medium, which was then
rapidly cooled by being immersed in cold water and inoculated at once with a few bits of the
crushed comedones previously washed in sterile salt solution. Melted sterile vaseline was
next poured over the surface of the bouillon to prevent access of air, the whole operation being
gone through in a very short time so as to prevent reabsorption of oxygen. In two to five
days there was a distinct growth in which acne bacilli could be seen in large number along
with the staphylococcus albus in the majority of cases. A subculture was then made in
bouillon anærobically by the same method and after three or four days' incubation a growth
was obtained. in which staphylococci were few and acne bacilli predominant. This broth
culture was heated and carbolised and used as the vaccine. It was combined with a vaccine
made from the coexisting staphylococcus albus whenever the latter was found in fairly large
numbers in the aerobic cultivation.
An interesting case that was treated during the year was one of acne indurata affecting
mostly the neck and the lower part of the face. After suppuration small but prominent and
unsightly scars were produced. Whilst in England some time previously the patient had
been treated with an autogenous staphylococcus vaccine with marked success. The trouble
had however recurred after coming out to India. Cultures made from the pustules gave a
growth of staphylococcus albus only, no acne bacilli being detected. A course of combined
autogenous staphylococcus albus vaccine and stock acne vaccine was however given and the
eruption cleared up rapidly leaving the skin smooth and healthy.
'TABLE IV.
Acne.
Serial
num-
ber.
Case
num-
ber.
Medical Officer.
Disease.
Vacci ne.
Result.
1
47 The Resident Medical Officer, General
Hospital, Madras.
Acne . . .
Acne bacillus . . . Improved.
2
55 The Fourth Physician, General Hos-
pital, Madras.
Do. . . .
Do. . . . Cured.
3
56 The Officer Commanding Station Hos
-pital, Poonamallee.
Do. . . . Acne bacillus and
staphylococcus
albus.
Improved.
4
58
. . .
Do. . . . Staphylococcus albus. Much im
-proved.
5
99 The Officer Commanding Station Hos
-pital, Poonamallee.
Do. . . . Acne bacillus and
staphylococcus
albus.
Cured.
6
101
Do. do.
Do. . . . Staphylococcus albus. No effect.
7 105 Dr. P. Lakshmanan, Quilon . . . Do. . . .
Do.
Not known.
8
147 The Resident Medical Officer, General
Hospital, Madras.
Do. . . . Acne bacillus and
staphylococcus
albus.
Do.4
4