113
TIRUPATI.
WATER SAMPLES TAKEN—4TH SEPTEMBER 1912.
Remarks.—The river Kalyani was dry when samples were taken. The supply is intermittent.
There has been about 3.90 inches of rain since last analysis. The pumping of
water from the new experimental well into the equilibrium ball valve cistern,
began from 22nd June 1912, but it was stopped from 3rd September 1912 owing
to some disorder in the steam-engine.
Bacteriological .Analyses.
SAMPLE 1.—A SHALLOW WELL ON THE BANK OF KALYANI RIVER
TO THE EAST OF HEADWORKS.
N u m b e r . L a c t o s e .
S a c c h a r o s e .
D u l c i t e . A d o n i t e . I n u l i n e .
V o g e s a n d P r o s k .
I n d o l .
M o t i l i t y .
G r o u p .
Name of Bacilli.
Group and
Remarks.
Total colonies per cc. on agar at 37° C.—1,040.
Bile salt lactose, medium.
+
-
1 tube of 20 cc.
1
...
1 + +
- - - -
+
-
IV B. Coscoroba
Group I, nil.
2 tubes of 10 cc.
2
...
2 + +
- - - -
+
-
IV
Do.
"
II, nil.
3
+
+
- - - -
+
-
IV
Do.
"
III, nil.
3
"
5 cc.
3
. . .
4 +
+
- - - -
+ -
IV
Do.
" I V , 1 0 .
3
"
1 cc.
3
. . .
5 + +
- - - - + -
IV
Do.
3
" .1 cc.
1
2
6 + +
- - - -
+
-
IV
Do.
3
" .01 cc.
...
3
7 + +
- - - -
+
-
IV
Do.
Fœcal bacilli in 1 cc. and upwards.
8 + +
- - - -
+
-
IV
Do.
Class I, nil.
9 + +
- - - -
+
-
IV
Do.
" II, 10.
No vibrios in 100 cc.
10 + +
- - - -
+
-
IV
Do.
" III, nil.
SAMPLE II.—A SHALLOW WELL ON THE BANK OF KALYANI
RIVER TO THE WEST OF HEADWORKS.
1 + +
-
+
- - - -
IV B. Gasoformans
Group I, nil.
2 + +
-
+
-
+
- -
IV B. Lactisacrogenes
"
II, nil.
3 + +
-
+
- - - -
IV B. Gasoformans
" I I I ,
n i l
.
Total
colonies per cc. on agar at 37° C .—
2,200.
4 + + -
+
- - - -
IV
Do.
" IV, 10.
5 + +
-
+
- - - -
IV
Do.
Bile salt lactose, medium.
+
-
6 + +
-
+
-
+
- -
IV B. Lactisacrogenes.
1 tube of 20 cc.
1
...
7 + +
-
+
- - - -
IV B. Gasoformans.
8 + +
-
+
- - - -
IV
Do.
Class I, nil.
2 tubes of 10 cc.
2
...
9 + +
- + - - - -
IV
Do.
" I I , 1 0 .
3
"
5 cc.
3
. . .
10 + +
-
+
- - - -
IV
Do.
" III, nil.
3
" 1 cc.
3
. . .
3
"
.1 cc.
. . .
3
3
" .01 cc.
.
3
Fœcal bacilli in 1 cc. and upwards.
No vibrios in 100 cc.
SAMPLE III.—FROM NEW EXPERIMENTAL WELL.
1 +
+
+ +
-
+
- -
III B. 67.
2 +
- - - - -
+ + I B. Grunthal
Group I, 6.
Total colonies per cc. on agar at 37° C.—4,500.
3 +
- - - - -
+
-
I B. Vesiculosus
"
II, nil.
4 +
- - - - -
+
-
I
Do.
" III, 2.
Bile salt lactose, medium.
+
-
5 +
+ - - - -
+
-
IV B. Coscoroba
"
IV, 2.
6 +
- - - - -
+
-
I B. Vesiculosus.
7 +
+ - - - -
+ + IV B. 106.
1 t u b e o f 2 0 c c
.
1
. . .
8 + + + +
-
+ +
-
III B. 76
Class I, nil.
9
+
- - - - - + -
I B. Vesiculosus.
" II, 3.
2 tubes of 10 cc.
2
. . .
10 +
-
-
-
- -
+
-
I
Do.
" I I I ,
6 .
Undetermined, 1.
3 " 5 cc.
3
. . .
3
"
1 cc.
3
. . .
3
"
.1 cc.
3
...
3
" .01 cc.
. . .
3
Fœcal bacilli in .1 cc. and upwards.
No vibrios in 100 cc.
SAMPLE IV.—COLLECTING CHAMBER.
1 +
- - - - -
+
-
I B. Vesiculosus
Group I, 10.
2 +
- - - - -
+
-
I
Do.
"
II, nil.
Total colonies per cc. on agar at 37° C.—2,420.
3
+ - - - - -
+
-
I
Do.
" III, nil.
4
+ - - - - -
+
-
I
Do.
"
1V, nil.
5 +
-
- -
-
-
+ -
I
Do.
Bile salt lactose, medium.
+
-
6
+
- - - - -
+
-
I
Do.
7
+ - - - - -
+
-
I
Do.
1 tube of 20 cc.
1
. . .
8 +
- - - - -
+
-
I
Do.
Class I, nil.
9
+ - - - - -
+
-
I
Do.
" I I ,
n i l
.
10
+
-
-
- - -
+
-
I
Do.
" III, 10.
2 tubes of 10 cc.
2
. . .
3
"
5 cc.
3
...
3
"
1 cc.
3
. . .
3
"
.1 cc.
1
2
3
" .01 cc.
...
3
Fœcal bacilli in 1 cc. and upwards.
No vibrios in 100 cc.
33 c.10