24
Testing.—On the 76th day after the serum injection, the animal was
inoculated with 1 c. c. virulent blood. Actual weight 336 lbs.
Progress.—On the evening of the 6th day after inoculation, the temper-
ature rose to 41.4°C. and then gradually returned to normal limits. No
symptoms of rinderpest supervened and the animal continued to feed well.
Remarks.—The temperature gradually rose until it attained 41.6°C. and
then returned to normal limits. The animal was therefore still protected after
76 days.
The remaining animals included in this series will be subjected to testing
with virulent rinderpest blood at varying intervals according to the results
recorded. Up to the present 10.4 c.c. of protective blood-serum at 20 c. c.
per 600 lbs. have protected an animal for a period of 76 days, as against
100 c. c. or more in South Africa granting protection for 60 days only, and
the hyper-immunised serum used in these experiments was from 10 to 11
months "in vitrio."
TABLE No. 3.
Showing the details connected with the above experiments as far as at present worked out.
Serial No.
1
2
3
4
5
6
7
8
9
10
11
12
Laboratory No.
220
142
258
259
260
261
278
279
280
281
282
283
Computed weight
by
measure-
ment.
lbs.
396
415
338
313
346
318
310
303
325
351
348
310
Date of injection
of Protective
Serum.
16th Feb. 1900 .
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Serum
weight
per 600 lbs.
c.c.
10
10
20
20
50
50
100
100
150
150
200
200
Actual quantity
of serum accord-
ing to computed
weight.
c. c.
6.5
6.9
11.2
10.4
29.4
26.5
51.6
50.5
81.1
87.6
116.0
103.3
Weight by
machine when
tested
with
virulent rinder-
pest blood.
lbs.
403
418
351
337
336
412
354
349
399
-
Date of testing
with virulent
blood.
27th Feb. 1900 .
31st March 1900.
Do.
3rd May 1900 .
Do.
30th May 1900 .
Do.
30th July 1900 .
Do.
...
Quantity of viru-
lent blood in-
jected.
c. c.
1
1
1
1
1
1
1
1
1
...
Time which
elapsed between
injection of Se-
rum and testing.
Days.
12
43
43
76
76
103
103
164
164
...
REMARKS.
Very mild attack.
No reaction.
Slight temperature
reaction.
Very slight temper-
ature reaction.
Slight temperature
reaction.
Ditto.
Ditto.
Ditto.
Ditto.
*Poisoned by eating
Ayár leaves.
*Natural order—Ericacæ.
Andromeda (Pieris) ovalifolia.
Vernacular name.
[A. Capricida. Don.]
Ayár.
Very common in Kumaon ;
Laboratory 7,500 feet elevation.
Andromeda (Cessiopa fastigiata.)
[A. Cupressiformis. Wall.]
Kamba.
Grows nt Miiani and Pindari,
12,000 feet elevation.
20. Serum Testing Experiments.—There is much danger in field inocula-
tions of cattle by the simultaneous method of overdoing the dose of protective
serum, so as to avoid any mortality ; for the natives will not consent to any
loss. Government animals should receive according to their body weight,
only sufficient serum to counteract a severe attack of the disease, otherwise
the protection afforded would be only a passive one, the immunising effect of
which would pass off in a very short period, viz., 2 to 3 months ; whereas the
animals which presented a fairly well marked thermal reaction accompanied
by vesicles, no matter how few, would receive a protection which would extend
over a period of five years or probably for life.
As 97,000 cubic centimetres of hyper-immunised serum required to be
tested with regard to its protective power per 600 lbs. body weight before being
issued, it was determined to inoculate five head of plains cattle by the
simultaneous method with doses of 5, 10, 15, 20, 25 c. c. per 600 lbs. Careful
notes being kept concerning each case, noting specially the symptoms presented,
vesicles on the gums, tongue, etc. These animals will have to be kept for a
long period and then be re-tested, for those which survive the simultaneous
method will be protected in the inverse ratio to the quantity of protective
serum they receive, i.e., according to the severity of the rinderpest symptoms
displayed in each case.