37
On February 28th, 1895, the animal was inoculated on the muzzle by means of a scratch,
and a trace of soiled blood, containing numerous hæmatozoa, was lightly smeared over the sur-
face of the wound. The blood was derived from a guinea pig, primarily inoculated from Horse
No. CXXV.
The period of incubation lasted eight days, the disease ran its course in 53 days, but was
not of a virulent type. The hæmatozoon was present in the blood for 39, and absent during the
intermissions for 14, days.
Water drunk.—During health 11.73 litres were taken daily, the amount increased during
the latent period to 13.78 litres. In the paroxysms it further increased to 18.97 litres, and in
the intermissions fell to 15.5 litres. The average for the whole period (89 days) being about
the same as the latter figure (15.68).
Water in food.—In addition to the above, 4.21 litres, or 9.27 lbs. were taken in daily as
moisture in the fodder and grain, equal to a total 15.94 litres or 35.06 lbs. ingested. The ratio
of the water egested to ingested was in health 1: 1.73, it decreased in the latent period to
1: 1.91, in the paroxysms to 1 : 2.02, and in the intermissions to 1: 2.04, while for the whole
period of 89 days it averaged 1: 1.93.
The amount of water ingested during health was 1: 19.78 of the body-weight, whereas
during the last paroxysm the ratio was 1: 9.07.
Food.—This consisted of hay and lucerne only. The animal weighed at the commencement
of the experiment 705.6 lbs. and at death 575.0 lbs. The intestines contained as much as 84.75
lbs. of dung, and to this must be added 27.5 lbs. passed on the day of death, or a total of 112.25
lbs. dung equal to a little more than 1/6 of the body-weight in health, or 1/5 at death.
This fact made it a point of considerable difficulty to ascertain the true loss at different
periods during the experiment.
The loss for the whole period was 130.6 lbs. or 18.50 % of the body-weight.
Urine.—The mean daily quantity of urine voided in health was 1,829 c.c., in the latent
period it increased to 2,309 c.c. and nearly double (4,233 c.c.) during the paroxysms. In the
intermissions there was again a fall to 3,869 c.c. and for the whole period of 89 days, the mean
daily average was 3,246 c.c. The maximum average daily output during any of the paroxysms
was 5,768 c.c. and the minimum 2,817 c.c., while in the intermissions it was 7,310 c.c. and
3,355 c.c. respectively.
Urea.—The average daily excretion of urea in health was 23.54 grammes, it increased in
the latent period to 28.86 grammes, in the paroxysms it amounted to 52.02 grammes, and in the
intermissions it further increased to 65.75 grammes, the maximum during any of the latter periods
being 130.56, and the minimum 47.59 grammes. For the whole period the mean daily average
was 43.14 grammes.
Hippuric Acid.—During the 28 days of health preceding inoculation the average daily
excretion amounted to 27.05 grammes ; in the latent period it was 27.94 grammes. In the
first paroxysm it fell to 9.46 grammes, and during the first intermission, lasting one day only,
there was an absence of hippuric acid. In the following paroxysms No. II, lasting eight days,
traces of hippuric acid appeared on two days only, viz., 3.9 and 3.2 grammes, respectively. For the
remainder of the disease, which in all passed through seven paroxysms and six intermissions, no
more hippuric acid was excreted.
Albumen.—During this case the urine was free from albumen.
Total Solids in the Urine.—The mean daily output during 89 days was 255.25 grammes.
The maximum mean daily quantity 263.72 grammes was passed during the six intermissions, and
the minimum was passed in the health period, viz., 240.42 grammes. The largest quantity passed
on any one day was 340.45 grammes in paroxysm VI, and the smallest quantity 138.37, passed
during health. The ratio of the total solids in the different periods were —
Health.
Latent period .
Paroxysms
Intermissions
Whole period
.. 1.00
.. 1.07
.. 1.08
.. 1.09
.. 1.06
Dung.—The average quantity passed daily throughout the whole case of 89 days was
21.69 lbs.; the mean daily averages in the different periods were as follows:—
Health
Latent period
Paroxysms
Intermissions
,.. 23.52 lbs.
.. 22.56 lbs.
,.. 21.38 lbs.
,.. 18.32 lbs.
Temperature and Humidity of the Atmosphere.—This investigation was carried out during
the hot season—February, March and April—in the Deccan. The maximum and minimum tem-
peratures in the shade during the whole period of 89 days were 106.5° and 48° F., the mean
maximum and minimum being 97.5° and. 54.7° F. The mean humidity taken at 8 A.M. daily
during this period was 44, with a maximum of 81, and minimum of 17.
B 563-10