18
sun for two days, and then kept for 12 days hung up in a room. The tempera-
tures during the period of drying were those given in experiment No. IV.
Result.—The temperature rose on the ninth day, and the animal had
suffered from a typical attack of rinderpest which terminated fatally on the
ninth day of the disease.
Remarks.—Here again the animal was exposed to natural infection from
the day after he was inoculated, owing to two other animals of a different series
of experiments contracting the disease in the same shed. As the temperature
rose on the seventh day, which is just within the extreme limit of the incubation
period given by South African observers, although a longer one than I have
ever seen in the inoculated disease in India, it is just possible that he got the
disease from the skin extract injection, but the experiment to be related
presently in the third series, in which a lime-treated skin of a more recent
date than the one used in this experiment failed to convey the disease in an
uninfected shed, taken together with the unusually long incubation period
of the disease, point strongly to the infection in the case under discussion
having been due to close relationship with other diseased animals, and not to
the virus having been preserved in the lime-treated skin.
Experiment No. IX.—A bull, aged 4, was inoculated with 10 c.c. of saline
extract of the skin of an animal that had died of inoculated rinderpest, and
which had been dried in the shade for 28 days, during which the maximum
temperature in the shade had varied between 47° and 69°, and the minimum
between 34° and 50° F.
Result.—The temperature, pulse and respiratory curves remained normal
for 10 days, and no symptoms appeared during that period of time, but the
animal having been left in an infected shed soon afterwards contracted the
disease naturally, showing that he was susceptible to it, but that the skin in
question did not contain the virus.
Remarks.—This second series of experiments, then, showed that a skin
which had been dried in the sun for four days and kept ten days more was
void of infection, as were other, portions of the same skin which had been
similarly treated, only with the addition of salt, while a part which had been
treated with lime gave doubtful results, owing to the shed in which the animals
had to be placed having been previously infected. Another skin which had
been dried in the shade gave negative results after one month.
THIRD SERIES OF EXPERIMENTS.
33. Experiment No. X.—A bull, aged 1½, was inoculated on April 3rd with
a saline extract, 5 c.c, of the skin of an animal that had died of inoculated
rinderpest, and which had been dried in the sun for two days and then kept in
a room for six days more. The maximum and minimum shade temperatures
during the two days the skin was drying were 74°, 75°, 53° and 54° F.,
respectively, while the maximum registered by the solar radiation thermometer
on the same days were 141° and 157° F.
Result.—The temperature, pulse and respiratory curves remained normal
for ten days and no symptoms appeared. The animal was subsequently inocu-
lated with virulent blood in another shed and contracted the disease, showing
that he was susceptible to it, but that the skin used was free from infectious
properties.
Experiment No. XI.—A small bull, aged 5 years, was inoculated subcu¬
taneously with 5 c.c. of an extract of the skin of an animal that had died of
rinderpest, and which had been dried in the shade for two days, and kept hung
up in a room for eight days more. The maximum temperature in the shade
during the three days when the skin was being dried was 68°, 72° and 70°,
respectively, and the minimum temperatures were 53°, 50° and 51° F.
Result.—The -temperature, pulse and respiratory curves remained normal
for ten days, while no symptoms appeared. The animal was subsequently
inoculated with virulent rinderpest blood and died of the disease, proving that
he was susceptible to the disease, and that the skin had lost all infectiousness.
Experiment No. XII.—A small bull, aged 6, was inoculated subcu¬
taneously with a saline extract of small pieces of the skin of the same animal
as was used in experiment No. XI, and which had been treated with lime in
the shade for two days, and then kept in a room hung up for eleven days more.
Result.—The temperature, pulse and respiratory curves remained normal
for ten days, and no symptoms appeared. The animal was subsequently