5
and 7 c. c. for plains per 600 lbs., has remained unaltered, which is a further
proof that sera may be kept at the temperature of Muktesar without undergoing
deterioration as a result.
9. Quetta.—On the 20th July, 1902, 2,800 c. c. of No. XII sera were
issued to the Superintendent, Civil Veterinary Department, Baluchistan and
Sindh, Quetta. It was retained in his office, in a dark room, during a period
of 9¼ months and was exposed to a maximum shade temperature rising to
98.6° F. This serum was prepared at Muktesar during November, 1901, to
February, 1902, and tested on hill animals during the months of March and
April, 1902, when its protective value was standardized at 5 c. c. for plains and
90 c. c. for hill animals, per 600 lbs. body weight. On the 12th May, 1903, it
was received at Muktesar and after retesting, it was found to have deteriorated
in protective power from 5 to 7 c. c. per 600 lbs. body weight. The question
now arises as to whether serum forwarded during the hot season to a station a
long distance off, as Quetta from Muktesar, will deteriorate in value, if it has to
pass through a portion of the country exposed to a very high temperature, as
for instance by Ruk Junction, Jacobabad, Sibito Quetta. In a previous report
it was found that serum retained in the Superintendent's office, Rangoon,
exposed to a maximum temperature of 104° F., was equally efficacious either
by the serum alone method or by the simultaneous method, after injecting the
Laboratory standard doses. As no evidence is at our disposal as to what length
of time this serum was exposed in the plains previous to its being deposited in
Quetta, it is at present impossible to give a final opinion on the course of its
deterioration in value.
10. Lucknow, Jalaun, Meerut and Moradabad experiments.—In accord-
ance with the orders of the Government of India conveyed in their Resolution
No. 21—108-2, dated 25th August, 1902, Revenue and Agricultural Depart-
ment, to the effect that " the Government of India have observed with interest
the results of the experiments carried out in different localities to ascertain the
strength of doses required to secure protection in different breeds of cattle and
hope that Dr. Lingard will continue his researches in this direction ", arrange-
ments were made to carry out experiments with Lucknow, Meerut, and Jalaun
cattle at the Bareilly Depôt during the winter months.
A full and detailed report of the results of these experiments was forwarded
to Government on the 11th May, 1903, together with temperature charts of the
respective animals and an album containing photographs of nearly all the
cattle (68 out of 70) utilized during such experiments.
The following short resumes of the results obtained in the cattle of the
respective districts of the United Provinces experimented with are here
recorded.
11. Experiments with Lucknow bulls.—Of the three Controls inoculated
with virulent blood, one succumbed to severe disease, while a second passed
through a fairly severe attack, the third showing a thermal reaction but no
other symptoms. Of the 21 animals inoculated by the simultaneous method
all those which received 7 c. c. (the standardized dose for plains cattle) and
above, of the protective serum recovered, while of the 6 animals which received
1 and 2 c. c. short of the standardized dose, one succumbed to a severe form of
the disease, two presented severe symptoms and recovered and others showed
more or less thermal reactions and no symptoms.
This series of experiments prove that probably a dose of 6 c. c. per 600 lbs.
might have been sufficient to protect by the simultaneous method but that 7
c. c. per animal by the serum alone method would have been ample to secure
immunity during an outbreak of disease in the district.
12. Jalaun bulls.—One animal, out of 4 controls, inoculated with virulent
blood succumbed to a severe form of disease, while a second presented a
thermal reaction with slight symptoms, i.e., only 50 per cent. exhibited signs
of rinderpest at all, while the other half showed no symptoms. As these
animals only varied between 2 and 4 years of age, it would appear that the
animals obtained from the Jalaun District possessed a considerable degree of
either acquired or hereditary protection, probably the latter. Of the 19
animals varying in age between 2 and 4 years and one only of 6 years which
received protective serum and blood by the simultaneous method all recovered
even those 6 which received 1 to 2 c. c. below the standardized dose of 7 c. c.