74.
Filariasis-.
4. During the microscopical examination of the blood of the various species of fish, the
' Chachari' obtained from stagnant water, on Remount Depot Estate, supplied the most un-
expected results, the circulation of one specimen literally teeming with a small variety of
hæmatozoa, while in several other specimens the organisms were noted as swarming.
5. Two horses posted in stables at the Mohand Pass were discovered to be the subjects
of the Filaria papillosa.
Three horses at Karnal exhibited symptoms of partial paralysis in one of which, an acute
case, the paralysis became complete and death followed with hyperpyrexia. In the circulation
of this animal numerous Embryo filaria were observed microscopically.
Two bullocks out of thirty examined exhibited the Filaria in their circulation, 126 worms
being found in four specimens of blood derived from one and 60 in the other, or a ratio per
specimen of 31.5 and 15, respectively.
The complete recovery of Bullock which was the subject of Surra, Filariasis, and Intermit-
tent fever in December 1895.
Karnal.
2. Rainy Season Tour.
On September 7th, 1896,I arrived at Saharanpur and proceeded to the Mohand Pass.
where in 1894 over 80 horses succumbed to Surra. The blood of some 135 horses in all, stationed
at Mohand, Tunbara, Landibara and Asarori was subjected to microscopical examination,
but only two animals were found to be the subjects of Surra and these at the latter station.*
The infected animals, brought from the North Punjab, were the property of the European Firm
of Dak proprietors, who have for several years past taken the precaution to boil the water
before giving it to their animals in this locality. The fodder issued by this firm is dry
grass, collected during the cold season, November to January, from high ground ; the corn in
the form of oats is likewise cleaned and screened.
The stables at Asarori are situated on the banks of a river, at an elevation of 3,000 feet,
about half a mile from the crest of the north-eastern slope of the outer range of the Siwaliks.
Although little or no rain in comparison with other seasons has fallen between Saharanpur
and Landibara, the last chowki near the summit of the outer Siwaliks range, nevertheless
the normal quantity has fallen this season between Asarori and Rajpur to the north-east*
The stables at Asarori, where the majority of the animals belonging to the native owners
are quartered, are nearly on a level with the bed of the river and the ground is swampy.
On the other hand the European firm have constructed their buildings on the
highest available site above the road, and introduced the above mentioned precautionary
measures ; nevertheless the only equines affected with Surra on the 49 miles of road between
Saharanpur and Rajpur up to September 9th were two of the eight horses stationed at
Asarori, 3,000 feet elevation, which were thought to be protected from the insanitary
surroundings.
At Saharanpur similar investigations to those previously recorded during my hot season
tour were made, but the results proved negative.
The microscopical observations regarding water, blood of fish, flies and ticks made during
my tour in the hot season were repeated in detail during this visit.
Water.—Numerous infusoria were found in the different samples submitted to examination
but no Trypanosoma was observed.
Depôt Pond.
Old Jumna River.
Old Jumna River.
Fish,—Cacheri (Trichogaster faciatus, or a variety of this species). In the blood of eleven
specimens examined the small variety of the hæmatozoon
was found present varying from numerous to few; and
absent from the circulation of seven.
The small variety of hæmatozoon was present in one and
absent from the blood of two fish examined.
Grisauli or Girai (Ophiocephalus Striatus).—No hæma¬
tozoa were found in the blood of the three specimens
examined.
Jumna River.
Singhi (Macrones seenghala.)—The blood of three specimens of this species was submitted
to examination with the result that the large variety of hæma-
tozoon was found to be numerous in one and absent from
two fish.
Specimens of blood were likewise taken from fish of different genera, as was the case
during the investigations conducted during the hot season, but the results proved again to be
of a negative character.
Flies.-—The body fluids of numerous flies, embracing a variety of genera and species
caught on the bodies of animals, and in the vicinity of swamps and stagnant water, were sub-
mitted to microscopical examination, but with negative results.
Ticks.—Specimens of Ixodes were taken from horses and dogs. Usually the horses in
this district suffer much from these acariases, but only two specimens were found on 153
horses examined for them.
* After my visit to this Station (Asarori), four additional horses succumbed to Surra belonging Messrs.
Smith and Rodwell. The disease next visited Landibara and four animals died the property of Native owners.
Both these chowkis are by thick Jungle and situated close to the river.