53
On first weighing the animal registered 374 lbs., and 31 days later 347, although it
was supplied with hay ad libitum and as much water as it would drink.. The mean weight
for this period was 362 lbs. After inoculation, and during the different stages of paroxysms
and intermissions, there was a continuous loss, until at death the body weighed 297 lbs„ a
total loss of 77 lbs.
At the post mortem the intestines were found to contain 68.25 lbs. of dung, which
represented in health 18.02 per cent. and at death 22.98 per cent. of the body-weight. The
body showed a total absence of any visible adipose tissue.
Water drunk.—The average daily quantity of water drunk during the period of observa-
tion was 9.26 litres; it increased nearly two litres in the latent period (11.09), and reached
a maximum of 16.82 litres during Intermission III. After this as the disease progressed
the average quantity taken was reduced, until in Intermission VII it was only 1.02. The
mean for the paroxysms and intermissions was practically identical, viz., 10.90 and 10.24
litres, respectively. For the whole case the average daily quantity was 10.04 litres.
Water in food.—In addition to the above .845 kilos (l.86lbs.) were taken in daily as
moisture in the fodder, equal to a total of 10.89 kilos (23.96 lbs.) ingested. The ratio of water
egested to ingested was in the observation period 1: 1.16; it increased in the latent period to
1 : 1.41, whilst during the paroxysms it was practically the same as in the observation,
slightly increasing in the intermissions. For the whole period of 82 days it was the same
as in the observation.
Food.—This consisted of hay only. The ration contained a daily average of 0.416 per
cent. of Nitrogen. During the whole case the animal ingested 1,581.532 grammes of
Nitrogen and egested in the Dung 1,488.680 grammes, giving 92.852 grammes as assimilated
Nitrogen. The total Nitrogen excreted in the Urine during the same period was 698.957
grammes. Deducting the amount of assimilated Nitrogen from this, 606.105 grammes are
left, which represent the actual Nitrogen lost. Converting the Nitrogen eliminated to its
equivalent as flesh, we obtain a loss of 25.698 kilos (56.537 lbs). The total decrease in body-
weight during the whole period was 35 kilos (77 lbs.) so that the loss by Nitrogen was equal
to 73.42 per cent. the remaining 26.58 per cent. being due to Carbonic Acid, moisture, etc.
No trace of adipose tissue was to be observed at the post mortem.
Urine.-The
average quantity passed daily in the observation period was 1040 c.c.,
which was increased after inoculation to 1,594 c.c., and in the paroxysms it averaged 1453 c.c.,
increasing in the intermissions to 1,598 c.c. The average quantity passed daily throughout the
whole case was 1499 c.c.
The maximum quantity, 2,020 c.c., was passed during Intermission II, and throughout the
case it varied from that amount to as little as 610 c.c., in the period of preliminary observa-
tion. The specific gravity varied from 1,058 to 1,030, while the Urine taken from the bladder
at post mortem was only 850 c.c., containing no Hippuric Acid and only 2.2 per cent. of total
solids including 0.71 per cent. of Urea.
Urea.—The quantity of Urea excreted by this animal was exceedingly small, the average
daily output in the observation period being only 3.211 grammes, and in the latent period
3.190 grammes. In the paroxysms this amount was quadrupled, 12.50 grammes being
passed daily, but it fell again in the intermissions to 8.57 grammes daily, and for the whole
period was 7.01 grammes. It will be noticed that when the Urea in the paroxysms increased
the Hippuric Acid slightly decreased, and vice versâ, in the intermissions.
Ratio of Urea to Urine.—During the period of observation at rest the average ratio was
1:323.9, in latent period 1 : 499.6, in the paroxysms 1:116.2, and in the intermissions 1:186.3,
while for the whole period it was 1 : 213. This ratio shows a very marked difference to the
results of our investigations with equines, but there is a corresponding rise during the disease
in both instances.
Hippuric Acid.—In the period of observation this animal passed 49.32 grammes, which
increased in the latent period to 61.89 grammes. In the paroxysms and intermissions the
amounts were 55.70 and 55.69 grammes, the respective periods lasting 15 and 31
days. The mean for the whole period 82 days was 53.66 grammes. The maximum daily
quantity, 90.72 grammes, was passed in Intermission III, and for the last 21 days of the
case the amount excreted gradually decreased to 20.00 grammes, while the Urine passed during
the 12 hours preceding death and that removed from the bladder (post mortem) contained no
Hippuric Acid at all.
Of the total Nitrogen in the Urine during the different periods, the following table
shows the percentage excreted:—-
TABLE XLVI.
BUFFALO II.
PERIODS.
Observation
Latent Period
Paroxysms
Intermissions
Whole Period
.
.
.
.
.
.
.
.
.
.
As Urea.
%
25.81
20.03
51.48
40.18
37.98
Hippuric
Acid.
%
66.28
64.96
38.35
43.62
49.10
N H 3 and
Kreatmin,
etc.
%
7.36
15.01
10.17
16.20
12.32
TOTAL.
%
100
100
100
100
100