21
Bile—
(i) Injection of Bile (unmixed), 10 c. c., 5 c. c., 2 c. c., 1 c. c.
(ii) Injection of Bile mixed with rinderpest blood (1:10) 10 c. c.,
5 c. c., 2. c. c., 1 c. c.
(iii) Injection of bile—
(a) Heated to 100°.
(b) Carbolic acid 0.5 per cent.
(c) Other substances.
(d) Filtered.
Mixed with rinder¬
pest blood 1: 10 to
1: 100.
(iv) Injection of bile of healthy animals mixed with rinderpest
blood in any proportion (1 : 100, 1 : 1,000 ?).
(v) Bile animals No. 1 and No. 2 proved for duration of immunity
by injection of rinderpest blood after 2, 3, 6 months.
(vi) Injection of fluid, taken out of the swelling of a bile injected
animal.
(vii) Bacteriological conditions of the bile.
(viii) Injection of bile and injection of rinderpest blood after 8 to
10 days. These animals must be proved in the same
manner as No. 5.
(ix) Experimental feeding with bile and also with blood and also
with mixtures of (bile and blood), (serum and blood).
Immunity—
Important to prove duration of immunity in—
(a) Animals immunized only by bile.
(b) Animals immunized by bile followed by rinderpest blood
injection.
(c) Animals immunized with mixture of bile and rinderpest
blood.
Duration of time to elapse before injunction of rinderpest blood to prove
immunity or otherwise 3 to 6 months.
Proof may also be established by utilizing the serum of such an animal.
Hypothesis.—If animal has high and active immunizing serum, then it
is supposed to have immunity of a long duration. In order to prove course of
immunizing power of the serum and to make C.
Take filtered serum from spontaneous immunized bullock—
(a) about the period when temperature falls.
(b) again after 10, 20, 30, 40, 50, etc., days.
This is injected into healthy animals and 24 hours later 0.2 c.c. rinder-
pest blood.
Immunizing power, when lost or partially lost : is it possible by injec-
tion of large quantity of rinderpest blood to restore the high immunizing
power in the same animal ?
Microbes.—"Materies morbi" of rinderpest.
Examination of blood and aseptic bile (a) Microscopical, (b) by cultiva-
tion in most diverse ways and culture fluids and media.
Any microbe found in this way must produce the disease under condi-
tions, which exclude absolutely any other kind of infection, and must be
excluded from healthy, etc., animals.
18. Animals protected by different methods and retained in order to dis-
cover the period during which immunity was conferred.—After the following
animals had been subjected to inoculation with rinderpest material by differ-
ent methods, in June 1897, and had passed through a slight or severe form of
the disease as the case may be, they were drafted off to the herd there to
remain until several years had elapsed when they would have been tested with
virulent rinderpest blood, in order to ascertain whether the protection had
passed off altogether, or what degree of immunity was retained respectively.
It is generally accepted by the most enlightened ryots that, when once an
animal recovers from an attack of rinderpest, it is unlikely for it to again con-
tract the malady during the ensuing 5 or 6 years, if at all ; for the average
limit of life, in the case of bovines in India, seldom exceeds 10 years. During
my absence from India on sick leave, the officiating Imperial Bacteriologist
tested all the animals with virulent rinderpest blood, with the following results.