27
temperature attained a maximum on the 6th day following inoculation and on
the 7th the animal was off feed, and succumbed to a mild attack of rinderpest
on the 8th. The mucous membranes were normal and no external symptoms
were present.
Result.—Insufficient dose of serum.
Buffalo, aged 2 years, body weight 511lbs., was inoculated by the simul-
taneous method with pony serum No. I (20 c. c. per 600 lbs.) and 1 c.c. virulent
blood. The temperature of this animal rose to 40.2°C. thirteenth day follow-
ing inoculation, but it never presented any symptoms of rinderpest with the
exception of a small vesicle on the lower lip. The weather during the period
occupied by these experiments was particularly cold and inclement, conse-
quently the plains animals suffered considerably from the cold and several
animals suffered from gastro-enteritis and one succumbed to the effects of the
weather.
Result.—Seventeen (17) c. c. serum protected the animal.
At a later date the pony received 2,000 c. c. virulent blood, and after the
necessary period blood was abstracted and serum prepared, which was tested
upon a plains animal.
Plains bull, aged 3½ years, body weight 381 lbs., was inoculated by the
simultaneous method (14.25 c.c. per 600lbs.), with pony seram No. II prepared
20 days after 2,000 c. c. virulent blood had been injected. The temperature
which previous to inoculation registered 38.6°C. on the evening of 5th day,
rose to 40.1°C., but again fell to 39.2°C., on the following morning. During
the next 15 days, the highest evening temperature was 39.8°C. and lowest
morning 38.5°C. A very cold spell of weather occurred on the arrival of the
animal under experiments and others from the Terai. This animal and several
other healthy ones presented symptoms of gastro-enteritis and one succumbed.
Result.—9 c. c. of pony serum No. II prepared by the slow method, pro-
tected a plains animal, body weight 381 lbs.
Remarks.—The pony used for this experiment was too old for the purpose,
viz., 22 years. However 17 c. c. of the serum prepared after a 1,000 c. c. dose
of virulent blood protected a buffalo of 511 lbs. body weight, and after an
increased injection of 2,000 c. c. virulent blood, 9 c. c. granted immunity to a
plains bull of 381 lbs.; the results of the experiment must therefore be consi-
dered highly successful.
(b) Can the blood of a dog be made to furnish a hyper-immunised serum
capable of furnishing protection to bovines and buffaloes, when
the animal is subcutaneously injected with virulent rinderpest
blood, in large increasing doses,at intervals?
A dog, several years old, body weight 28 lbs., was inoculated with 500 c.c.
of virulent rinderpest blood. A slight reaction followed on the 4th morning,
the temperature attained a maximum of 40.7°C. and then gradually fell. On
the 14th day after the first injection of blood, a second dose of 1,000 c. c. was
administered subcutaneously. The animal died suddently some 40 hours later.
At the autopsy performed several (6) hours later, 20 c. c. of blood was collected
from the heart and mixed with 2.5 c. c. of a 5 per cent. carbolic solution. The
immunizing power of the blood was ascertained by the following experiment.
Plains bull, aged 4 years, body weight 374 lbs., was inoculated by the
simultaneous method with 20 c. c. dog's blood (collected 6 hours post-mortem)
and 0.5 c. c. virulent rinderpest blood. The amimal's temperature which was
38.4°C. at the time of the operation rose to 39.6°C. on the following evening,
but during the next 12 days no symptoms of rinderpest supervened.
Result.—The 20 c. c. of blood equivalent to about 12 or 13 c. c. of serum
must have possessed considerable immunizing power, for the control animal
presented marked symptoms of rinderpest on the 2nd and 3rd days of the
disease, whereas the experimental animal showed no symptoms of the disease.
Dog serum, therefore, can furnish a hyper-immunized serum possessing little
less protective power than that obtained from some bovines.