2
The subcutaneous injection of protective serum would appear to produce
an immediate effect on the lining membrane of the gall-bladder and hence an
increased production of the Nucleo-proteid.
(6) The marked increase in N. P. in animals injected by the simul-
taneous method occurs on the 7th and 8th day of disease and on the 11th
day following inoculation. On comparison of this result with that obtained
in Control Hill bulls, the sudden increase of N. P. appears one day later ;
this may be explained by the facts that the effect of the injection of the
protective serum appears to prolong the period of incubation.
(7) The amount of N. P. in the bile of healthy young bovines is at its
minimum previous to inoculation. The maximum amount in Serum Testing
animals is attained on the 12th day of disease and 14th day following
inoculation, after that date a gradual diminution in the amount of N. P.
appears to take place, but the minimum according to our data, never falls
to that found in health.
It is but reasonable to account for the difference in the percentage of
N. P. found at the height of the disease and when death takes place
during recovery, on the supposition that the N. P. is re-absorbed into the
system, in order to act as a recuperative or defensive agent, for it is difficult
to suppose that nature provides this increase of a highly organised product
simply to be voided from the system as a waste material.
(8) The marked rise in N. P. in Hill buffaloes injected with rinderpest
blood only, takes place on the 7th day of disease, not on the 6th day as in
bovines, for the first symptoms of the disease in such animals appear on the 7th
or 8th day following inoculation.
(9) A comparison of the amount of N. P. in the bile of healthy and
susceptible full grown plains and hill cattle and buffaloes with that in the
bile of the same animals at death, after subcutaneous inoculation with the
materies morbi of Rinderpest and Anthrax, indicates a considerable increase.
Taking as a unit the amount of N. P. in health, the multiple at death for each
class of animal were as follows :—
Rinderpest
Anthrax
Plains bulls
{ Hill do.
.
.
Do. buffaloes
{ Plains bulls
Hill do.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
4.20 to 105.21
3.12 to
7.22
1.52
6.13
3.40
(10) The bile collected from the gall bladders of Plains and Hill cattle
and buffaloes, which had not been inoculated, but which succumbed to Gastro-
Enteritis from bad feeding during the hot and famine seasons, was found to
contain a much larger amount of N. P. than in health, as may be seen in the
following table :—
ANIMALS.
10 Hill buffaloes . . .
56 Hill bulls . . .
13 Plains bulls
. . .
%N.P.in
grammes, in
healthy
young
animals.
1
0.0250
0.0175
0.0124
% N. P. IN GRAMMES, FOLLOWING
GASTRO-ENTERITIS.
Minimum.
2
0.1406
0.0640
0.0968
Maximum.
3
1.0880
2.2220
1.0360
Mean.
4
0.3591
0.3693
0.3906
Percentage
ratio of
N. P.
columns
1 and 4.
5
1 : 14.36
1 : 21.10
1 : 32.14
It would thus appear that a mechanical irritation or simple inflammation of
the lining membranes of the stomachs and intestines of bovines and buffaloes,
is sufficient to stimulate and cause an increase in the output of N. P. from the
m. m. of the gall bladder without the presence of a disease due to a particulate
contagium.
(11) N. P. may be precipitated from rinderpest bile. It may be
collected in a fairly pure state after reprecipitation and washing on several
occasions.
NOTE.—N. P. indicates Nucleo-proteid of bile, removed direct from the gall-bladder.