223
SUBCUTANEOUS INOCULATION OF ANIMALS WITH SOILED FISH BLOOD.
HORSE No. XXXIX.
Grey country-bred horse, aged.
10th August 1891.—Temp. A.M.—P.M. 36.2°C. Blood healthy.
11th.—Inoculated with blood mixed with 0.6 per cent salt solution, taken immediately after death from 3 speci-
mens of the Rhynchobdella aculeata " Baum " (Hindustani) " Warm " (Maratha). Vide Day's Fishes, page 338. Plate
LXXII. No. I. Temp. A.M. 35.8°C., P.M. 36.1°C. Blood healthy.
12th.—There is an irregular-shaped raised tumour at the seat of inoculation, which exactly resembles that produced
by the inoculation of Horse Surra blood, or from Rat's blood. Temp. A.M. 35.6°C. NO hæmatozoa. Temp. P.M.36°C.
13th.—The swelling at the seat of inoculation has increased and now presents a raised tense tumour, somewhat
circular in form, which measures nearly 2" in diameter and is exceedingly tender on manipulation. Temp. A.M. 35.7°C.
Hæmatozoa absent. Temp. P.M. 36°C.
14th.—The general health of the animal continues unaltered. The swelling and œdema at the seat of inoculation
has increased in extent since yesterday and measures 3¼" in diameter; and in the centre of it, the tumour, stands
raised an inch or more above the surrounding tissues. There are a few small petechiæ on the conjunctival membranes ;
but the visible mucous membranes are free from any yellow tinge. The appetite is good and the excretions natural in
character. Temp. A.M. 35.8°C. Hæmatozoa absent. Temp. P.M. 36.7°C.
15th.—The general condition of the animal is unchanged, and the swelling and œdema remains in, statu quo.
Temp. A.M. 37.1°C. Hæmatozoa absent. Temp. P.M. 37.2°C.
16th.—Condition unchanged. Temp. A.M. 37°C. Hæmatozoa absent. Temp. P.M. 37.1°C.
17th.—Condition unchanged. Temp. A.M. 37°C. Hæmatozoa absent. Temp. P.M. 37.5°C.
18th.—The general condition of the animal is unchanged. The œdema at the seat of inoculation has certainly
decreased in extent since yesterday. Temp. A.M. 37.2°C. Hæmatozoa absent. Temp. P.M. 37.2°C.
19th.—Condition unchanged. The swelling at the seat of inoculation is still more reduced in size, and the tender-
ness of the part is disappearing. The tumour can be lifted up bodily with the skin, showing that it does not involve
the underlying structures. Temp. A.M. 36.8°C. Hæmatozoa absent. Temp. P.M. 37.2°C.
From the 20th August to the 25th, no hæmatozoa were observed in the blood, but the animal rapidly became weak,
lost its appetite, and died at 9 P.M. on the 25th.
Autopsy — There was an entire absence of any pathological changes referable to the disease.
Remarks.—This animal was inoculated on the 11th August with blood from three fish containing hæmatozoa,
very much resembling those found in Horse Surra blood. There was some swelling and œdema accompanied with
tenderness on manipulation at the seat of inoculation on the following and subsequent days ; but after a week, it com-
menced gradually to subside. No hæmatozoa were found in the blood of the horse, and the post mortem revealed
no pathological changes.
HORSE No. XLIII.
Grey entire, country-bred, aged.
19th
20th.
21st-
September 1891.
— „
—
„
—Temp.
„
„
A.M. 39°C., P.M.
37.3°C
37.2°C
38.4°C. Blood
„ 38.1ºC.
„ 37.3°C.
healthy.
„
„
22nd.—Temp. A.M. 35.7°C. Inoculated subcutaneously over the right scapular region with blood mixed-with
salt solution containing hæmatozoa resembling those found in " Surra." The blood was taken immediately after
death from a specimen of the Rhynchobdella aculeata—"Baum"
(Hindustani) "Warm" (Maratha). Vide Day's
Fishes, page 338. Plate LXXII. No. I. The Fish weighed 171 grammes. Temp. P.M. 37.4°C.
23rd.—There is a raised swelling at the seat of inoculation together with tenderness on manipulation. Temp. A.M.
36.8°C. Hæmatozca absent. Temp. 1 P.M. 38.4°C ; 5 P.M. 38°C.
24th.— The swelling at the seat of inoculation has increased and the tumour is tense and more tender on manipula-
tion. Temp. A.M. 37.2°C. Hæmatozoa absent. Temp. 1 P.M. 38.6°C ; 5 P.M. 37.3°C.
25th.—The swelling at the seat of inoculation remains in statu quo. Temp. A.M. 36.2°C. Hæmatozoa absent.
Temp. 1 P.M. 37.5°C ; 5 P.M. 37.2°C.
26th.—The swelling at the seat of inoculation is considerably reduced in size; the tenderness has disappeared.
There are several small ecchymoses on both conjunctivæ, the right more especially, and there is a muco-purulent dis-
charge from the left nostril. Temp. A.M. 36.3°C. Hæmatozoa absent. Temp. 1 P. M. 37.6°C ; 5 P.M. 37.1°C.
From September 27th to November 23rd, when the animal died, no hæmatozoa were observed in the blood, and the
temperature was normal, except from the 8th November to the 13th inclusive, when it rose slightly, ranging between
37°C. and 39.5°C. On the 23rd October and 11th November, numerous well-marked ecchymoses were present on both
membranæ nictitantes, and on the latter date the mucous membranes were tinged yellow.
Autopsy.—The body is somewhat emaciated and the tissues are tinged a light orange colour, a few ecchymoses of a
dark-red hue are present on the membranæ nictitantes. There is also slight œdema of the hind limbs.
Thorax.—The pleural cavities are free from fluid and there are no pleuritic adhesions.
Lungs.—The surface of the right lung is for the most part of a pink colour, dotted over with a number of small purple
coloured sub-pleural extravasations. The apex is the seat of a localized emphysema and near the apex of the organ is a some-
what circular-shaped patch of a dark-red colour. Under the visceral pleuræ covering this area is deposited a thin layer of