DIAGNOSIS OF JOHNE'S DISEASE BY THE USE OF JOHNIN.
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1. Guernsey. Age unknown. Showing wasting and diarrhœa. Rl (also avian
tuberculin) NRI, (tuberculin) K 4. No T lesions and 2 gp inoculated from
bronchial glands were not infected. Advanced lesions of J in intestine, J seen in films
of intestine and cultivated therefrom.
2. Jersey cow. Age unknown. Scouring rather badly and thin. R1. NR1
(tuberculin). K5. No T lesions. Ileo-cæcal valve very congested and smears
from it showed scanty J, J also seen in sections and cultivated from the intestine.
3. Black poll steer, about 2 years. R1. Slight R1 (tuberculin). Thin, badly-
developed animal. K15. No T lesions. Advanced lesions of J in intestine and J
seen in films. Cultures not made.
4. Jersey. Second calver. At times was in poor condition and showed some
diarrhœa and falling-off in milk yield. R1 (anal), 57, 87 (anal). R 140 (tuberculin)
K168. T lung and bronchial glands. No lesions of J, but J present in films. Sec-
tions of the ileo-cæcal valve showed very numerous J and typical histological changes.
Cultures were not made.
5. Guernsey cross. Aged cow. In fair condition at first. R1 and 101. Slight
R 21 (tuberculin) K166 and prior to this had scoured and become emaciated. Found
to be pregnant. Lesions of T in lungs and glands and on pleura. Advanced lesions
of J in intestine and J seen in large numbers in films and sections from intestine.
Cultures not made.
6. Shorthorn heifer, rising 2 years. NR1, R101 and 214. NR214 (tuberculin).
K250 at time when starting to waste. No lesions of T seen and 2 gp injected from the
bronchial, pharyngeal and sub-maxillary glands, and 2 gp injected from the intestinal
glands did not become infected. Lesions of J in the intestine, small numbers of J seen
in films and in large numbers in sections of the small intestine. Cultures not made.
7. Shorthorn heifer, about 18 months old NR1, indefinite R101, R214, NR311.
R (tuberculin) 21 and 214. Wasted after day 311 and K399. The intestine and glands
alone received for examination. Extensive lesions of J in intestine, J seen in films
and isolated in cultures from the intestine.
(NOTE.—" Indefinite R 101 " = increase of skin thickness from 9 to maximum
of 16 mm., but the swelling was firm and circumscribed.)
8. Cross-bred cow. NR1, 97 and 206, R 306. NR(tuberculin) 1 and 206. Calved
day 295 and then began to scour and waste. K316. No T lesions and 2 gp injected
from bronchial and intestinal glands did not become infected. Extensive lesions
of J in intestine, J seen in anti-formin preparations, in sections and isolated in
culture.
9. Cross-bred Shorthorn cow. Age unknown. Bad clinical case. Rl (also tuber-
culin). Slight R1 (avian tuberculin). K8. No T lesions. Lesions of Jin intestine
numerous J seen in films from intestine and cultivated therefrom.
10. Shorthorn cross. Yearling heifer. R1. 97 and 206, NR 311. R206 (tuber-
culin). Started to waste just prior to day 353 when K. T lesions present in submaxillary
gland only and 2 gp inoculated from it became infected. 2 gp inoculated from intes-
tinal glands did not become infected. Advanced lesions of J were present in the in-
testine, J were seen in films and in nnormous numbers in sections of the intestine
and were isolated in culture.
N