DIAGNOSIS OF JOHNE'S DISEASE BY THE USE OF JOHNIN.
395
20. Shorthorn. Yearling heifer. NR1, 101, 311, 401, 486 and 575, R214, NR 575
(avian tuberculin). NR (tuberculin) 214 and 575. Wasted and showing diarrhœa
after day 486. K 579. No T lesions and 2 gp inoculated from bronchial and medias-
tinal glands did not become infected. Typical lesions of J present in intestine,
J seen in films from intestine and gland and were cultivated from these sites.
(NOTE.—" R214 " = increase in thickness of skin from 6 to 17 mm., swelling
diffuse. At the other tests a maximal thickening of a shade over twice the original
was reached on two occasions only, but on one of these the concentrated medium
was used as a control and gave practically the same reading.)
21. Friesian. Third calver. From alleged T-free herd. R1 and 11. K 44.
Scouring badly a few days before slaughter, No T lesions and 3 gp inoculated from
bronchial, pharyngeal, submaxillary and mesenteric glands did not become infected.
Lesions of J in intestine, J seen in films from intestine and cultivated therefrom.
22. Friesian. Second calver. From alleged T-free herd. R1. K44. Scouring
before slaughter. No T lesions and 2 gp inoculated from bronchial and mediastinal
glands did not become infected. Lesions of J in intestine, J seen in films from
intestine and cultivated therefrom.
23. Shorthorn. Third calf, day 163. R1, 85, 154, 225 and 246. R (tuberculin)
1, 85 and 225. Remained in fair condition. K 295. T lesions present in bronchial
glands ; 2 gp inoculated from the intestinal glands did not become infected. Lesions
of J present in intestine, J not seen in anti-formin preparations of intestine and
intestinal gland, but were isolated from both situations in culture.
24. Shorthorn cow. NR1,R 85 (anal). NR (tuberculin) 1 and 85. Had wasted
before test on day 85. K 92. T lesion in one mesenteric gland, inoculation of which
produced T in gp. Lesions of J not definite ; J seen in anti-formin preparations and
in small numbers in sections from the small and large intestine ; J cultivated from
an intestinal glands.
25. Shorthorn cow. Aged. Had shown intermittent but not copious scouring.
R1 and 57. R (tuberculin) 1 and 57. Calved day 109. K 147. Lesions of T in lungs
and bronchial glands and in one or two of the mesenteric glands. Ileo-cæcal valve
showed some patchy congestion ; J seen in anti-formin preparations of the intestine
and in very small numbers in sections of the small intestine; J isolated in culture.
26. Shorthorn cow. R1 (also anal), 101 (also anal, 214. R (tuberculin) 1
and 214, K250, when starting to lose condition. Animal pregnant. No T lesions
seen and 2 gp injected from bronchial, pharyngeal and submaxillary glands and
2 gp from intestinal glands did not show infection. Slight lesions of J in intestine,
a few J seen in films and sections of intestine and J were isolated from intestine.
27. Red poll. From alleged T-free herd R1 (anal), NR91 (anal), R202 and 343.
In poor condition and very loose for some time prior to slaughter on day 351. No T
lesions seen and 2 gp injected with material from bronchial glands showed no T
lesions when killed. Lesions of J in intestine, J seen in antiformin preparations and
in very small numbers in sections of the intestine and isolated from the intestine.