164
after the first appearance of the hæmatozoon in the blood, and nineteen after inoculation. At the Autopsy, extensive
pathological lesions were found involving the heart, kidney, liver and spleen, and the two latter were also found to be
very considerably enlarged.
EXPERIMENT No. II.-RABBIT II.
21st, July 1892.—5-20 P.M. Inoculated the animal subcutaneously in the right inguinal region with 0.75 c.c. of
blood containing hæmatozoa taken from the heart of Horse No. LXII, immediately after death. This
horse had been inoculated with rat's (Mus Decumanus) blood. Temp. P.M. 39.4 °C.
22nd.—Temp. A.M. 40.0 °C. Hæmatozoa absent. Temp. P.M. 40.3 °C.
23rd.—There is no swelling or œdema at the seat of inoculation. Temp. A.M. 39.4 °C. Hæmatozoa absent. Temp.
P.M. 40.3 °C.
24th.—Temp. A.M. 40.0 °C. Hæmatozoa absent. Temp. P.M. 40.2 °C.
25th.—Temp. A.M. 39.8 °C. The white corpuscles are increased in number, but there are no hæmatozoa in the
blood. Temp. P.M. 40.5 °C.
26th.—Temp. A.M. 41.2 °C. There are a few hæmatozoa in the blood. Temp. P.M. 41.6 °C.
27th.—Temp. A.M. 40.4 °C. Hæmatozoa absent. Temp. P.M. 40.8 °C.
28th.—Temp. A.M. 40.1 °C. Hæmatozoa absent. Temp. P.M. 40.4 °C.
29th.—Temp. A.M. 40.4 °C. P.M. 40.7 °C. Hæmatozoa absent.
30th.—Temp. A.M. 40.3 °C. P.M. 40.2 °C.
„
31st.—Temp. A.M. 40.2 °C. P.M. 40.4 °C.
1st August—Temp. A.M. 40.2 °C. P.M. 40.7 °C. Hæmatozoa Few.
2nd.—Temp. A.M. 40.8 °C. P.M. 41.0 °C. Hæmatozoa Few
3rd.—Temp. A.M. 40.8 °C. P.M. 40.7 °C. Hæmatozoa absent.
4th.—Temp. A.M. 40.8 °C. P.M. 41.3 °C.
„
5th.—Temp. A.M. 40.8 °C. P.M. 41.0 °C.
„
The red blood corpuscles do not form rouleaux, and the white ones, which on the 25th ultimo appeared to be
increased in numbers, have now returned to the healthy standard.
6th.—Temp. A.M. 40.7 °C. P.M. 41.8 °C. Hæmatozoa absent.
7th.—Temp. A.M. 41.1 °C. P.M. 41.6 °C.
„
The red corpuscles do not form rouleaux, and are for the most part lozenge-shaped. The white contain one or
more nuclei and numerous highly refractile granules.
8th.—Temp. A.M. 40.2 °C. P.M. 40.5 °C. No hæmatozoa in the blood.
9th.—Temp. A.M. 40.4 °C P.M. 40.5 °C. Few
10th.—Temp. A.M. 40.6 °C. P.M. 41.6 °C. „
11th.—Temp. A.M. 41.1 °C. P.M. 41.4 °C. „
„
„
12th.—Death occurred in the early morning.
RESULTS NEGATIVE.
BULL No. I.
This animal was brought to the Laboratory on the 5th March 1891 and was kept under observation until
the 24th of the same month, during which time it appeared quite healthy and normal in every respect. On the
latter date, it was inoculated subcutaneously in the right thigh with 1.75 c.c. of blood, containing numerous hæmato-
zoa, drawn from the jugular vein of Horse No. XIX, previously inoculated with the same disease. The incubative
period occupied 5 days. Two short paroxysms occurred during the first few days of the disease, but although the
blood was further examined daily until June 17th, eighty-five days after inoculation, no hæmatozoa were
found. On this latter date, two guinea pigs were inoculated with 0.2 c.c. of blood derived from the bull, and in each
instance the hæmatozoon followed after a period of ten days. During the interval of 78 days which elapsed between
June 17th and September 3rd, 1891, no organisms were discovered in the blood of the bull, although several cover
glass specimens of it were examined daily. On the 163rd day after the primary inoculation, two more guinea pigs
were injected with 0.2 c.c. of bull's blood, and ten days latter the organism of Surra was found in the general circula-
tion of both animals. Negative results followed the inoculation of guinea pigs with small quantities of the bull's blood
on the 234th and 267th days. As seventeen months had elapsed from the date of the primary inoculation of the animal
on March 24th, 1891, and no organisms had been discovered in its blood on microscopical examination since the 2nd
April 1891, it was resolved on the 22nd August 1892 to re-inoculate the animal with a more virulent type of Surra,
obtained by passing the hæmatozoon of the rat (Mus Decumanus) through the horse and donkey. One cubic centimetre
of blood containing very numerous and active hæmatozoa, drawn from the internal jugular vein of Donkey No, I, was
subcutaneously injected over the left scapular region. On the 28th of August 1892, a peculiar hæmatozoon was found
in the blood, but for that day only. The animal put on flesh and appeared in robust health. Two guinea-pigs inocu-
lated, 21st September, with blood drawn from the jugular vein, failed to reproduce the disease. The observations
were continued from the last appearance of the organism, viz., 28th August 1891 until the 30th June 1892, a period
of 306 days. The above observations included the daily records of temperature, pulse, respiration, together with the
examination of blood and urine. (See remarks Vol. I, page ccxxiii.)