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SUBCUTANEOUS INOCULATION AND RE-INOCULATION WITH SOILED FISH BLOOD.
RESULT NEGATIVE.
HORSE No. XLIX.
Black mare, aged, in fair condition and apparently healthy.
15th October 1891.—Inoculated subcutaneously with blood containing numerous hæmatozoa, mixed with normal
saline solution, taken immediately after death, from the hearts of several specimens of fish—Rhynchobdella aculeata
obtained from the waters of the River Mula-Mutha.
16th.—There is some swelling and œdema at the seat of inoculation. Temp. A.M. 37.3° C. No hæmatozoa. Temp.
P.M. 38.1° C.
17th.—The swelling and œdema at the seat of inoculation has increased in extent, and the tumour presents a some-
what circular form, and is tender on manipulation. Temp. A.M. 37.3° C. No hæmatozoa. Temp. 1 P.M. 37.7° C.; 5 P.M.
38.4° C.
18th.—The swelling and œdema at the seat of inoculation has decreased during the night, but the tenderness is still
present. There are no ecchymoses on the conjunctivæ. Temp. A.M. 37.4° C. No hæmatozoa. Temp. 1 P.M. 38.1° C.;
5 P.M. 38.3° C.
19th.—All swelling and œdema at the seat of inoculation has entirely disappeared, and there is only a slight thicken-
ing under the skin. There are no conjunctival ecchymoses. Temp. A.M. 37.6° C. No hæmatozoa. Temp. 1 P.M.
38.3° C.; 5 P.M. 38° C.
From the 20th October to the 28th there was no change in the condition of the animal. The temperature was
normal and no hæmatozoa were observed in the blood.
29th.—Re-inoculated the animal over both scapular regions, with blood (mixed with salt solution) taken from a fish
of the same species, as that used on the previous occasion. Temp. A.M. 37.3° C. No hæmatozoa. Temp. 1 P.M. 37.8° C.;
5 P.M. 38.2° C.
30th.—There are well marked petechiæ on the conjunctival membranes, and some swelling and œdema at the point
of inoculation. Temp. A.M. 37.3° C. No hæmatozoa. Temp. 1 P.M. 38°C.; 5 P.M. 39° C.
31st.—The swelling has considerably diminished at the seat of inoculation on the right side, and there is only slight
thickening of the tissues over and around the seat of inoculation on the left side. Temp. A.M. 37.4° C. No hæmatozoa.
Temp. 1 P.M. 38° C.; 5 P.M. 38.4° C.
From the 1st November to the 6th, the animal appeared in good health and no hæmatozoa were observed in the
blood ; but there was a gradual rise in temperature to 39.5° C. on the evening of the 6th.
7th.— There is a muco-purulent discharge from the right nostril observed this morning for the first time, but no
sign of ulceration in either nostril, and only very slight congestion of the vessels of the nasal septum, on the op-
posite side. There is continued gurgling in the intestines. The appetite is good. Temp. A.M. 40.1° C. No hæmatozoa.
Temp. 1 P.M. 40.5° C.; 5 P.M. 40.2° C.
8th.—Temp. A.M. 40.2° C. No hæmatozoa. Temp. 1 P.M. 40.4° C.; 5 P.M. 40.6° C.
9th.—The animal is losing flesh ; the appetite has fallen off. There is a slight yellowish tinge of the membranes
and a catarrhal discharge from both nasal passages. Temp. A.M. 39.6° C. No hæmatozoa. Temp. 1 P.M. 40.2° C.; 5 P.M.
40.5° C.
10th.—There are numerous large and dark-coloured ecchymoses on the left membrana nictitans, but the right con-
junctival membrane is quite clear. Temp. A.M. 39.7° C. No hæmatozoa. Temp. 1 P.M. 40.1° C.; 5 P.M. 40.4° C.
11th.—The membranes continue to present a yellowish colour. There are large petechiæ still present on the left
membrana nictitans, but they remain absent from that of the right. Temp. A.M. 40.0°C. No hæmatozoa. Temp. 1 P.M.
40.6° C.; 5 P.M. 41.4° C.
12th.—This morning there are large petechiæ on the right membrana nictitans, and they are also numerous on
the left. Temp. A.M. 40.3° C. NO hæmatozoa. Temp. 1 P.M. 40.6° C.; 5 P.M. 41.4° C.
13th.—Temp. A.M. 41° C. No hæmatozoa. Temp. 1 P.M. 41.5° C.; 5 P.M. 40.6° C.
14th.—Temp. A.M. 40.3° C. No hæmatozoa. Temp. 1 P.M. 40.4° C.; 5 P.M. 41.4° C.
15th.—The animal has, during the past few days, been gradually getting weaker and the appetite has remained poor.
Temp. A.M. 39° C. No hæmatozoa. Temp. 1 P.M. 41.3° C.; 5 P.M. 40.7° C.
16th.—At one o'clock this morning, the animal fell, and death ensued an hour later —2 A.M.
Autopsy.—Eight hours after death. The body is considerably emaciated. The tissues are all more or less tinged a
yellow colour, and large ecchymoses are present on the conjunctivæ.
Thorax.—Pleural cavities contain no fluid, and there are no pleuritic adhesions.
Lungs.—The superior surfaces of the lungs present a variegated appearance made up of varying degrees of pink,
reddish-yellow, and purple-blue. The apices are the seat of localized emphysema. Symmetrically placed in both lungs in
the middle of the anterior lobes, eight inches from the apices, are two circumscribed patches of consolidated tissue, dark-red
in colour, somewhat wedge-shaped and occupying in each case an area of about 6 or 7 square inches. The posterior lobes of
the organs present patches of yellow coloured exudation lying beneath the visceral pleuræ ; and in addition there are numerous
circular extravasations of blood dotted over the sub-pleural surfaces of the organs, more especially on the posterior half of the
left lung. On section, lying beneath the pleuræ covering these consolidated portions, there are layers of yellow (jelly-like)
exudation, which are found to dip down between the lobules and so divide them off, in some instances distinctly isolating
one lobule from another. A certain amount of fluid blood exudes from the cut surfaces of the tissue, but the latter
presents a healthy appearance.
The Pericardium contains two ounces of an amber-coloured fluid.
Heart.—The right side of the organ is dilated, the left is contracted.
The external surface presents
several patches of extravasation, the most extensive measuring 1.5" × .75," made up of small irregular-shaped