88
Liver.—Weight 11 lbs. 4 ozs. Dark in colour ; surface smooth and healthy, with the exception of a few scattered
circular bodies, dark-brown in colour, which on section are found to pervade the tissue of the organ.
Spleen.—Weight 2 lbs. 2 ozs. Normal in size. The surface presents numerous dark-red coloured but small
extravasations dotted about, mostly on the convex surface. On section, the tissues are firm and appear healthy.
Kidneys.—The Right one weighs 1 lb. 3 ozs. and the Left one, 1 lb. 3½ ozs. The tissues are of a pale greenish-
grey colour, but firm. The Stellate veins are injected at the base of the organs, but there are no petechiæ visible. On
section, the cortical portion presents very well marked congestion of the blood vessels.
Stomach. —Externally there is nothing noticeable. On section, the organ contains some semi-digested food. The
cuticular portion is healthy ; but the villous portion, midway between the line of demarcation and the pyloric extremity,
presents a raised lump above the general elevation of the surrounding coat, one inch in length and ¾th of an inch in
breadth.
Small Intestines.—There are several patches of petechiæ aggregated together, lying on the mucous membrane,
along the course of the small intestines. There is no ulceration.
Large Intestines—The cæcum and colon are full of fæces, but there are no petechiæ or ulcers.
Remarks.—This animal was inoculated subcutaneously with 0.5 c. c. of blood containing very numerous hæma-
tozoa, taken from Horse No. XXVIII, which latter had been injected with blood directly from the Rat (Mus decumanus).
The period which elapsed between the inoculation of the rat's blood and the appearance of the organism in the circu-
lation of Horse No. XXVIII was 29 days. In the present experiment, however, the period of incubation fell to 6 days,
only a fifth of the time occupied previously. This points to the fact that directly the organism of rat origin has become
acclimatized to its new surroundings in the horse, it is capable, on re-inoculation, of reproducing the disease in a fraction
of the time previously occupied.
HORSE No. XXXVI.
Dark Chestnut country-bred horse, aged, apparently healthy, and in fair condition.
Subcutaneous inoculation with soiled blood of rat, previously passed through one horse. Maximum local manifest-
ations at seat of inoculation on the 5th day. Appearance of the hæmatozoon after a latent period of 5 days, or 24 days
less than in the case of the direct rat inoculation. Death on the 4th day of the disease.
Date.
1891.
July
29 ...
„
30 ...
„
31 ...
August 1 ...
„ 2 ...
„ 3 ...
„ 4 ...
„ 5 ...
„ 6 ...
„ 7 ...
„ 8 ...
„
9 ...
„ 10 ...
Day of
Disease.
...
...
...
...
...
...
...
...
...
1
2
3
4
Temperature °C.
A.M.
35.5
35.6
35.5
35.7
35.8
36.4
36.5
36.0
36.5
36.2
36.8
37.4
37.6
1 P.M.
36.0
36.0
36.1
36.2
36.8
36.6
36.4
36.3
36.5
36.3
37.5
37.8
36.2
5 P.M.
36.1
36.2
36.3
36.4
37.2
36.7
36.4
36.4
36.5
36.4
37.7
38.0
35.8
Pulse.
A.M.
30
32
28
27
28
30
34
34
40
48
50
54
52
P.M.
36
36
30
32
30
36
36
38
47
56
60
60
58
Respiration.
A.M.
14
12
12
10
10
12
12
14
14
12
12
14
12
P.M.
12
16
10
14
16
14
16
14
16
16
18
18
14
Hæmatozoa
in Blood.
Healthy ...
„
...
„
„
„
...
Absent ...
„ ...
„ ...
„
Few
Very nume-
rous.
Swarming
Very num-
erous.
REMARKS.
2nd, 11 A.M.—The animal was inoculated
subcutaneously over the left shoulder
with .5 c. c. of blood, containing very
numerous hæmatozoa, taken from the
jugular vein of Horse No. XXVIII,
which had been previously inoculated
from the rat.
3rd.—There is a small circular swelling 1"
in diameter at the seat of inoculation,
which is surrounded by a narrow zone
of œdema.
4th.—Swelling and œdema increased
in extent, and tender on manipulation.
5th.—Swelling and œdema now measures
2.25"× l.5".
6th.—The swelling and œdema have still
further increased, and the tumour is
tense and tender on manipulation.
7th.—The swelling and œdema have some-
what subsided, and as a consequence the
hæmatozoon should have appeared in
the blood.
8th.—Swelling much reduced in size, and
now feels quite boggy.
9th.
Do.
do.
10th.—The animal became very weak du-
ring the day, and succumbed at 6.15 P.M.
Autopsy at 8 A.M., 11th August 1891.
Thorax.—There is no fluid in the pleural cavities or adhesions of the pleuræ.
Lungs.—Present some few small irregular patches of extravasated blood, lying under the visceral pleuræ about the
size of a two-anna piece. On section, both apices present a thin layer of yellow jelly lying between the individual lobules,
but this is entirely limited to the apices, and for a distance of about 6" posteriorly the tissue of the lobules presenting a
semi-solid condition, dark-red in colour. The remaining portion of both organs present on section a healthy appearance.
There is no fluid in the pericardial sac.
Heart.—Weighs 5 lbs. 2 ozs., and externally presents numerous and extensive extravasations under the visceral peri-
cardium, more especially posteriorly, lying on either side of the course of the coronary vessels. There are a few small ex-
travasations situated near the apex of the ventricles. Internally, on section, the right side of the heart is distended
with dark-coloured semi-solid clots, and there are a few sub-endocardial extravasations in the right auricle. Valves healthy.