NationalLibraryOfScotland/medical-history-of-british-india huggingface dataset:
106
RINDERPEST.
as follows:—Two to six litres of a solution of citrate of potash
are injected into the peritoneal cavity of a bull at the height
of an attack of Rinderpest. The blood of the bull is extracted
for the supply of virus and subsequently the fluid from the peri-
toneal cavity is also taken for the same purpose. By this means
two or three litres of virus from the peritoneum are obtained in
addition to the supply from the blood. It is stated that the
peritoneal exudate contains the Rinderpest material in more
virulent form than exists in the blood stream, and that the
washing provides an antigen of equal value to that obtained from
the blood. As a result of many repeated experiments we have
found that the serum prepared by injection of these washings
though of decidedly inferior potency to that prepared from
blood, is of considerable value, and can be used with advantage,
when added to the bulk of serum prepared in the ordinary way
from defibrinated virulent blood. It appears to me to be doubt-
ful whether the value of the peritoneal washings depends on a
superiority of virulence of the Rinderpest material said to be
contained in the peritoneal exudate. We have observed that
the amount of fluid in the peritoneal cavity of a bull at the
height of an attack of Rinderpest is extremely small, averaging
less than 100 c.c. It is highly improbable that 100 c.c. of in-
fected fluid diluted in 3,000 to 6,000 c.c. of a solution of citrate
of potash would provide an antigen of any great value. The
peritoneal injection is rapidly absorbed, about half the amount
disappears from the cavity within an hour after the injection.
It is more probable that, in addition to the process of ab-
sorption which takes place after the injection of citrate of po-
tash solution, a relatively large amount of body fluid contain-
ing the virus is attracted to the peritoneum and mixed with the
saline solution. This view is supported by the fact which we
have frequently noticed that the bulls prepared with peritoneal
injection supply a lesser volume of blood per body weight
than the bulls which had not had peritoneal injection. From
many experiments we have proved that a volume of virulent
blood diluted in an equal quantity of Pot. Cit. solution is much
RINDERPEST.
107
more rapidly and completely absorbed than in a similar volume
of defibrinated blood. An injection of citrated blood is gene-
rally completely absorbed within 24 to 48 hours. A massive
injection of defibrinated blood is not completely absorbed for
several days and many injections are never perfectly absorbed.
It is doubtful whether the virus absorbed after 24 to 48 hours
following the injection is of any utility in the formation of the
antibodies. From a series of experiments not yet complete
it appears probable that Citrate of Potash solution exercises
some special action on virulent blood and virulent peritoneal
fluid, by which it increases the value of the injection in the pre-
paration of serum.
If this view be found to be a correct explanation of the
action of the injection of a quantity of Citrate of Potash solution
into the peritoneal cavity, and of the value of the injection
of peritoneal washings in the preparation of the Rinderpest
serum, it would follow that a mixture of peritoneal fluid or of
virulent blood in Citrate of Potash solution, incubated for one
or two hours at body temperature would provide an antigen
equal in value to peritoneal washings. This question has also
been taken into consideration in the present experiments.
IMPROVED METHODS OF RINDERPEST SERUM PREPARATION.
Throughout this Report the letters V. B. express virulent
Rinderpest blood. Pot. Cit. a 0.5% or 1.5% solution of Citrate
of Potash.
The virulent blood used for test purposes was invariably
obtained from Hill bulls (Kumaun) taken at a height of an
attack of Rinderpest.
All experiments were controlled by simultaneous inocula-
tions of Hill bulls which in all cases died from Rinderpest.
SERUM PREPARATION-EXPERIMENT 2-A.
Buffaloes—V. B. + Pot. Cit. One injection at the negative
phase.
108
RINDERPEST.
In this experiment the virulent blood was taken from
buffaloes which had been inoculated with Rinderpest blood
from a Hill bull control. These control buffaloes had a mode-
rately marked attack of the disease, evinced by temperature
reaction, loss of appetite and wasting. No mouth lesions
appeared, and all recovered. One buffalo died after being bled,
probably from exhaustion. The serum making buffaloes were
similarly inoculated and passed through a like attack of Rinder-
pest. During the period of this attack they received a sub-
cutaneous injection of 1,500 c.c. blood taken direct into 1,500
c.c. of 0.5% Pot. Cit. They were bled for serum sixteen days
after the subcutaneous injection, and the serum of all the serum
making buffaloes was mixed and tested.
The serum tests show a remarkably potent serum. The
serum was tested on Hill bulls and on Plains buffaloes. Six
Hill bulls received a simultaneous injection of virulent blood
and of serum, two with 2 c.c. of serum per 600 lbs. body weight
(dose for Plains bulls). Two with 5 c.c. and two with 10 c.c.
Two buffaloes received 5 c.c. and two 10 c.c. None of these ani-
mals showed even the slightest temperature reaction. The
virulence of the blood was controlled by the death of other test
animals inoculated on the same date and also with the same
virulent blood.
This serum, judging from these tests, would probably have
protected at a dose of less than 1 c.c. per 600 lbs. body weight
(Plains).
TABLE OF RESULTS-EXPERIMENT No. 2-A.
SERUM 2-A.
METHOD OF PREPARING.—One injection of V. B. plus Pot. Cit.
RINDERPEST.
109
Reaction of Controls from which Blood was taken.
No. of
Buffa-
loes.
18
72
74
80
81
85
Temperature reac-
tion.
High
„
„
„
„
„
41.0 Max.
40.8
„
40.5
„
40.5
„
41.3
„
40.2
„
LESIONS.
Vesicles.
Nil.
„
„
„
„
„
Ulcers.
Nil.
„
„
„
„
„
Result.
Recovery.
„
„
„
„
REMARKS.
Died after bleeding.
Reactionof
SerumPreparingBuffaloes.
73
75
76
77
78
High
„
Slight
High
„
40.6 Max.
41.3
„
39.9
„
40.4
„
40.3
„
Nil.
„
„
„
„
Nil.
„
„
„
„
Recovery.
Died 7 days
after in-
jection.
Recovery.
„
„
Reaction of Serum Testing Animals.
Bulls.
269
281
267
271
277
274
266
Buff.
150
153
154
155
Nil.
„
„
„
„
Slight
Nil.
Nil.
„
Slight
„
39.1 Max.
38.9
„
38.9
„
38.8
„
39.5
„
40.1
„
39.6
„
38.8 Max.
38.5
„
40.0
„
38.9
„
Nil
„
„
„
„
„
„
Nil.
„
„
„
Nil.
„
„
„
„
„
„
Nil.
„
„
„
Recovery.
„
„
„
„
„
„
Recovery.
„
„
„
Dose per 600 lbs.
body weight.
10 c.c. ditto.
10 c.c. ditto.
5 c.c. ditto.
5 c.c. ditto.
2 c.c. ditto-
2 c.c. ditto,
2 c.c. ditto.
5 c.c. ditto.
5 c.c. ditto.
2 c.c. ditto.
2 c.c. ditto.
SERUM PREPARATION-EXPERIMENT NO. X.
Buffaloes V. B. and Pot. Cit. One injection at positive
phase.
This experiment was conducted on exactly similar lines
to experiment No. 1-A with the exception that the injection
of virulent blood and Pot. Cit. was given after the buffaloes
had recovered from the attack of Rinderpest.
The object of this experiment was to test the value of
serum prepared by one injection at a positive phase, that is
after recovery from an attack and when immune bodies had been
formed in the animal body as a result of the first attack.
The amount and method of injection and period of bleed-
ing were exactly similar in other respects to experiment 2-A.
110
RINDERPEST.
Two bulls with a dose of 2 c.c. per 600 lbs. body weight (Plains)
showed temperature reaction and one mouth lesions. Two at
a dose of 5 c.c. and two at a dose of 10 c.c. showed no reactions.
Two buffaloes at 5 c.c. and two at 10 c.c. dose showed no re-
action. This serum was protective at a dose of 2 c.c, per 600
lbs.
TABLE OF RESULT-EXPERIMENT No. X.
SERUM No. X.
METHOD OF PREPARING.—One injection V. B. and Pot. Cit.
Reaction of Controls from which Blood was taken.
No. of
Buffa
loes.
118
119
120
Temperature
reaction.
Nil.
Slight
„
40.0 Max.
40.0
„
LESIONS.
Vesicles.
Nil.
„
„
Ulcers.
Nil.
„
„
Result.
Recovery.
„
„
REMARKS.
Reaction of Serum Making Buffaloes.
22
79
83
Nil.
High
Slight
40.6 Max.
40.0
„
Nil.
„
„
Nil.
„
„
Recovery.
„
„
Reaction of Serum Testing Animals.
Bulls.
283
276
278
268
282
37
Buff.
152
156
149
151
Nil.
„
„
„
Slight
High
Nil
„
„
„
39.0 Max.
39.0
„
39.2
„
39.1
„
40.0
„
40.7
„
39.6
„
38.6
„
38.7
„
38.7
„
Nil.
„
„
„
„
9th day
Nil.
„
„
„
Nil.
„
„
„
„
11th day
Nil.
„
„
„
Recovery.
„
„
„
„
„
„
„
„
„
Dose per 600 lbs.
body weight.
10 c.c. ditto.
10 c.c. ditto.
5 c.c. ditto.
5 c.c. ditto.
2 c.c. ditto.
2 c.c. ditto.
5 c.c. ditto.
5 c.c. ditto.
2 c.c. ditto.
2 c.c. ditto.
RINDERPEST.
111
SERUM PREPARATION-EXPERIMENT NO. Y.
Buffaloes V. B. and Pot. Cit. Two injections at positive
phases.
The object of this experiment was to test the result of two
injections of diluted virulent blood, the first injection being
given at about six days after recovery from an attack, the
second injection at about sixteen days after the first. Buffaloes
were used, and the result of the test is comparable with both
experiments 2-A and X, and is further confirmed by a similar
series of experiments with Plains bulls No. 1-A, 1-B, 1-B2
and 1-D.
Two bulls which received a dose of 2 c.c. per 600 lbs. body
weight (Plains), both had severe attacks and died ; two with a
dose of 5 c.c. and two with a dose of 10 c.c. slight rise of tem-
perature only.
This serum was protective at about 4 c.c. per 600 lbs.
This result shows that serum prepared by two injections at
positive phases produces a serum of about half the potency
of that produced by a single injection at a positive phase, and of
one-quarter the value of a serum prepared by one injection at
a negative phase.
The results obtained by a similar series of experiments
in which Plains bulls were used in place of buffaloes gave the
same results:—
In the case of Buffaloes serum of one injection at the negative phase
„
„
„
„
„
„
„
„
„
positive
„
„ „ „ „ „
two „ „ „ „ ..
„
„ Plains bulls „ one
„
„
negative
„ ..
„
„
„
„
„
„
„
„
positive
„ ..
„ „ „ „ „ two
„
„
„
„
..
Protective
dose per
600 lbs.
1 c.c.
2 c.c.
4 c.c.
4 c.c.
7 c.c
12 c.c.
112
RINDERPEST.
TABLE OF RESULT-EXPERIMENT NO.Y.
SERUM No. Y.
METHOD OF PREPARING.—Two injections of V. B. and Pot. Cit.
Reaction of Controls from which Blood was taken.
No. of.
Buffa-
loes.
120
121
122
146
147
148
82
88
89
Bulls.
338
307
308
354
314
336
Temperature
reaction.
Slight
High
Slight
High
„
„
High
„
„
High
„
„
Slight
High
„
40.0 Max.
40.4
„
40.0
„
40.7
„
40.4
„
41.0
„
LESIONS.
Vesicles.
Nil.
„
„
„
„
8th day
Ulcers.
Nil.
„
„
„
„
„
Result.
Recovery.
„
„
„
„
..
Reaction of Serum Making Buffaloes.
40.3 Max.
40.5
„
40.0
„
8th day
Nil.
„
9th day
Nil.
„
..
Recovery.
„
Reaction of Serum Testing Animals.
40.7 Max.
40.6
„
40.4
„
40.0
„
40.8
„
41.0
„
Nil.
„
„
„
„
8th day
Nil.
„
„
„
„
9th day
Recovery.
„
„
„
Died on
13th day
Died on
11th day
REMARKS.
Died day after being
bled 2,000 c.c.
Died after injection.
Dose per 600 lbs.
body weight.
10 c.c. ditto.
10 c.c. ditto.
5 c.c. ditto.
5 c.c. ditto.
2 c.c. ditto.
2 c.c. ditto.
SERUM PREPARATION-EXPERIMENT NO. 1-A.
Plains bulls V. B. and Pot. Cit. One injection at the
negative phase.
This experiment was carried out on similar lines to experi-
ment 2-A. Plains bulls being used in place of buffaloes. The
injections were given during the period of attack and consisted
RINDERPEST.
113
of 1,500 c.c. V. B. bled direct into an equal volume of 0.5%
Pot. Cit. The Plains bulls, both controls and serum making
animals, passed through an attack of the disease which was
evinced by a slight rise in temperature, and in one case only by
mouth lesions. The attacks were much milder than in the case
of the buffaloes. Owing to a somewhat prolonged secondary
temperature reaction after the injection the bleeding for serum
was taken twenty-two days after the injection.
The serum from the three serum making bulls was mixed
and tested.
Three bulls received a dose of 2 c.c., one died and two
survived. Two bulls at a dose of 5 c.c., one showed a slight
temperature reaction only. Two bulls, at a dose of 10 c.c.,
showed no reaction.
The results show that the serum was protective at a dose
of 4 c.c. per 600 lbs. body weight (Plains).
The serum prepared from buffaloes by one injection dur-
ing period of attack proved about four times more potent
than serum prepared on similar lines from Plains bulls.
TABLE OF RESULTS.-EXPERIMENT No. 1-A.
SERUM No. 1-A.
METHOD OF PREPARING.—One injection of V. B. and Pot. Cit.
Reaction of Controls from which Blood was taken.
No. of
Bulls.
9
13
92
Temperature
reaction.
Slight
High
Slight
40.0 Max.
40.5
„
40.0
„
LESIONS.
Vesicles.
8th day
7th day
8th day
Ulcers.
Nil.
9th day
Nil
Result.
Recovery.
„
„
REMARKS.
8
114
RINDERPEST.
Reaction of Serum Making Bulls.
No. of
Bulls.
102
103
105
Temperature
reaction.
High
„
40.6 Max.
40.5
„
40.4
„
LESIONS.
Vesicles.
Nil.
„
„
Ulcers.
Nil.
„
„
Result.
Recovery.
„
„
REMARKS.
Reaction of Serum Testing Animals.
275
279
272
280
270
284
273
Nil.
„
„
High
„
„
„
39.5 Max.
39.4
„
39.5
„
40.2
„
40.7
„
41.1
„
40.5
„
Nil.
„
„
„
7th day
8th day
„
Nil.
„
„
„
8th day
10th day
„
Recovery.
„
„
„
Died on
12th day
Recovery.
„
Dose per 600 lbs.
body weight.
10 c.c. ditto.
10 c.c. ditto.
5 c.c. ditto.
5 c.c. ditto.
2 c.c. ditto.
2 c.c. ditto.
2 c.c. ditto.
SERUM PREPARATION.-EXPERIMENT No. 3-A.
Plains bulls V. B. defibrinated. One injection at a nega-
tive phase.
The object of this experiment was to compare the results
of a single injection of defibrinated blood as compared with
the result of a single injection of an equal volume of blood bled
into 0.5% Pot. Cit.
The experiment No. 3-A was identical with experiment
No. 1-A with the exception that defibrinated blood 1,500 c.c.
was injected in place of 1,500 c.c. V. B. drawn in an equal
volume of Pot. Cit. as in experiment No. 1-A.
Two bulls with a dose of 2 c.c. per 600 lbs. body weight
had a severe attack and died. Two bulls at 5 c.c., one had a
slight attack and recovered, the other a severe attack and died
on 16th day.
Two bulls at 10 c.c., one showed no reaction, the other a
slight rise of temperature only.
This serum was protective at a dose of about 7 c.c. per
600 lbs. body weight.
The serum prepared by the injection of diluted V. B. in
Pot. Cit. was about twice as potent as the serum prepared by a
similar volume of defibrinated virulent blood.
RINDERPEST.
115
The result of this experiment is further confirmed by
experiments Nos. 1-D and 3-D.
TABLE OF RESULTS.-EXPERIMENT NO. 3-A.
SERUM No. 3-A.
METHOD OF PREPARING.—One injection of defibrinated
V. B.
Reaction of Controls from which Blood was taken.
No. of
Bulls.
8
11
69
reaction.
High
Slight
..
40.9 Max.
40.0
„
40.0
„
LESIONS.
Vesicles
7th day
Nil.
Nil.
Ulcers.
8th day
Nil.
Nil.
Result.
Died after
being bled
2,200 c.c.
Recovery.
„
REMARKS.
Reaction of Serum Making Bulls.
1
14
16
326
327
337
332
313
358
Slight
„
„
High
„
„
Slight
„
High
39.6 Max.
40.0
„
40.0
„
Nil.
„
„
Nil.
„
„
Recovery.
„
„
Reaction of Serum Testing Animals.
40.5 Max.
40.5
„
41.3
„
39.8
„
40.1
„
40.7
„
Nil.
„
7th day
Nil.
7th day
Nil
Nil.
8th day
Nil.
8th day
Nil.
Recovery.
„
Died on
16th day.
Recovery.
Died on
9th day.
Died on
10th day.
Dose per 600 lbs.
body weight.
10 c.c. ditto.
10 c.c. ditto.
5 c.c. ditto.
5 c.c. ditto.
2 c.c. ditto.
2 c.c. ditto.
SERUM PREPARATION. –EXPERIMENT NO. 1-B.
Plains Bulls V. B. and Pot. Cit. One injection at a
positive phase.
This experiment was carried out on similar lines and with
a similar object as experiment No. X. In this case Plains bulls
were used.
116
RINDERPEST.
Two bulls at a dose of 2 c.c. died, two at a dose of 5 c.c.,
one had a slight attack, the other a severe attack and died on
the 11th day ; two at a dose of 10 c.c., one had a slight attack
and one severe, both recovered. This experiment was repeated
with similar results, see 1-B2.
The results of tests proved that the dose of this serum
necessary to protect was about 10 c.c. per 600 lbs. body weight
(Plains).
The comparison of the results obtained with serum No. 2-A
and No. X, and of serums Nos. 1-A and 1-B showed that serum
prepared by an injection of V. B. and Pot. Cit. at a negative
phase is more than twice as potent as serum prepared by one
injection of V. B. and Pot. Cit. at a positive phase.
TABLE OF RESULTS.-EXPERIMENT NO. 1-B.
SERUM No. 1-B.
METHOD OF PREPARING.—One injection of V. B. and
Pot. Cit.
Reaction of Controls from which Blood was taken.
No. of
Bulls.
109
110
116
Temperature
reaction.
High
„
40.6 Max.
40.4
„
40.4
„
LESIONS.
Vesicles.
Nil.
„
8th day
Ulcers.
Nil.
„
„
Result.
Recovery.
„
„
REMARKS.
Reaction of Serum Making Bulls.
4
12
101
Slight
High
Slight.
40.0 Max.
40.6
„
40.0
„
Nil.
8th day
Nil.
Nil.
..
Nil.
Recovery.
Died after
injection.
Recovery.
RINDERPEST.
117
Reaction of Serum Testing Animals.
No. of
Bulls.
349
333
316
350
346
318
Temperature
reaction.
High
„
„
„
„
„
40.6 Max.
41.2
„
40.5
„
40.9
„
40.5
„
40.7
„
LESIONS.
Vesicles.
Nil
„
„
6th day
6th day
8th day
Ulcers.
Nil
„
„
8th day
7th day
8th day
Result.
Recovery.
„
„
Died on
11th day.
Died on
10th day.
Died on
11th day.
REMARKS.
Dose per 600 lbs.
body weight.
10 c.c. ditto.
10 c.c. ditto.
5 cc. ditto.
5 c.c. ditto.
2 c.c. ditto.
2 c.c. ditto.
SERUM PREPARATION.–EXPERIMENT NO. 1-B2.
Plains bulls V. B. and Pot. Cit. One injection at a positive
phase.
This experiment was a repetition of experiment 1-B. The
injection of virulent blood was given at a later period than in
1-B that is twelve days after the attack.
Two bulls received a dose of 2 c.c. per 600 lbs. body weight
(Plains), one had a severe attack and died; other had a mild
attack and recovered. Two bulls with 5 c.c., both had a severe
attack and died. Two bulls with 10 c.c., one had a severe
attack, other a slight temperature reaction. Both recovered.
The tests gave a similar result to those of serum 1-B, though
the serum was slightly weaker than serum 1-B.
The serum was protective at a dose of about 12 c.c. per
600 lbs. body weight (Plains).
TABLE OF RESULT. —EXPERIMENT NO. 1-B2.
SERUM No. 1-B2.
METHOD OF PREPARING.—One injection of V. B. and
Pot. Cit. in a positive phase.
118
RINDERPEST.
Reaction of Controls from which Blood was taken.
No. of
Bulls.
123
125
126
Temperature
reaction.
High
„
„
40.6 Max.
40.8
„
40.8
„
LESIONS.
Vesicles.
8th day
7th „
8th „
Ulcers.
9th day
8th „
9th „
Result.
Recovery.
„
„
REMARKS.
Reaction of Serum Making Bulls.
15
99
104
344
348
317
306
310
32S
Slight
High
Slight
Slight
High
„
„
Slight
„
40.0 Max.
40.4
„
39.6
„
Nil,
„
„
Nil.
„
„
Recovery.
„
„
Reaction of Strum Testing Animals.
40.0 Max.
40.5
„
40.5
„
44.5
„
39.9
„
40.0
„
7th day
Nil.
8th day
7th „
9th „
6th „
8th day
i Nil.
10th day
9th „
9th „
8th „
Recovery.
„
Died on
13th day.
Died on
15th day.
Recovery.
Died on
11th day.
Dose per 600 lbs-
body weight.
10 c.c. ditto.
10 c.c. ditto.
5 c.c. ditto.
5 c.c. ditto.
2 c.c. ditto.
2 c.c. ditto.
SERUM PREPARATION.–EXPERIMENT NO. 1-C.
Plains bulls V. B. and Pot. Cit. One injection at a nega-
tive phase followed by a second injection at a positive phase.
In this experiment a first injection was given during the
period of attack. This was followed after an interval of sixteen.
days by a second and larger injection. The first injection was
given at a negative phase and the second injection at a positive
phase. The amounts of injections were similar to those in
experiment No. Y and experiment No. 3-D.
Two bulls which received a dose at 2 c.c. per 600 lbs. had
severe attacks and died ; two bulls with 5c.c. per 600 lbs., one
had a severe attack and died, the second had a rise of tempera-
ture only ; two bulls with 10 c.c., one had a slight temperature
reaction only.
The protective dose of this serum was about 6 c.c., per
600 lbs. body weight (Plains).
The serum was not so potent as the serum prepared by one
injection of a similar volume during the negative phase, which
gave a protective dose at 4 c.c. per 600 lbs.
RINDERPEST 119
TABLE OF RESULT-EXPERIMENT NO. 1 -C.
SERUM No. 1-C.
METHOD OF PREPARING.—Two injections of V. B. and Pot.
Cit.
Reaction of Controls from which Blood was taken.
No. of
Bulls.
5
7
9
139
140
141
142
Temperature
reaction.
High
„
„
„
„
„
40.5 Max.
40.5
„
40.0
„
40.5 „
40.2 „
40.5
„
40.7
„
LESIONS.
Vesicles.
8th day
7th „
8th „
7th „
7th „
8th „
8th „
Ulcers.
10th day
9th „
Nil.
8th day
8th „
9th „
9th „
Result.
Recovery.
„
„
Died on
9th day
after being
bled on
8th day.
Died on
10th day
after being
bled on
8th day.
Recovery.
REMARKS
Reaction of Serum Making Bulls.
70
71
95
„
„
„
40.5 Max.
40.6
„
41.0
„
Nil.
„
„
Nil
Recovery.
„
„
Reaction of Serum Testing Animals.
347
343
352
310
323
320
High
Slight
„
High
„
„
41.2 Max.
40.1
„
40.0
„
40.8
40.7
„
40.6
„
8th day
Nil.
„
6th day
Nil.
„
9th day
Nil.
„ 9th day
Nil.
„
Recovery.
„
„
Died on
12th night.
Died on
7th day.
Died on
8th night.
Dose per 600 lbs.
body weight.
10 c.c. ditto.
10 c.c. ditto.
5 c.c. ditto.
5 c.c. ditto.
2 c.c. ditto.
2 c.c. ditto.
120
RINDERPEST.
SERUM PREPARATION-EXPERIMENT No. 1-D.
Plains bulls V. B. and Pot. Cit. Two injections at positive
phases.
This serum was prepared by two injections of virulent
blood drawn into 0.5% Pot. Cit., both given at positive
phases.
The first injection was given six days after attack, and the
second after an interval of sixteen days.
Two bulls which received 2 c.c. per 600 lbs. had severe
attack and died, two bulls received 5 c.c., one had a slight re-
action, the other a severe attack followed by death; two bulls
with 10 c.c. both had a severe attack, one died, the other re-
covered.
The protective dose of this serum was about 12 c.c. per
600 lbs. body weight (Plains).
This serum was not so potent as that prepared (experiment
No. 1-B) by one injection at a positive phase. This gave a
serum of a protective close of 10 c.c. per 600 lbs. It was also
much inferior to serum obtained by one injection at a negative
phase. (Experiment No. 1-A, which was protective at 4 c.c.
per 600 lbs.).
TABLE OF RESULTS.-EXPERIMENT NO. 1-D.
SERUM No. 1-D.
METHOD OF PREPARING.—Two injections V. B. and
Pot. Cit.
RINDERPEST.
121
Reaction of Controls from which Blood was taken.
No. of
Bulls.
110
111
113
161
163
Temperature
reaction.
High
„
„
„
40.4 Max.
40.0
„
40.0
„
40.0
„
41.0
„
LESIONS.
Vesicles.
Nil.
„
„
8th clay
„
Ulcers.
Nil.
„
„
„
„
Result.
Recovery.
Died on
14th day,
was bled
2,000 on
8th day.
Recovery.
Died on
13th day.
Recovery.
REMARKS.
Reaction of Serum Making Bulls.
5
7
93
High
„
„
41.4 Max.
40.5
„
40.6
„
8th day
7th „
Nil.
10th day
9th „
Nil.
Recovery.
„
„
Reaction of Serum Testing Bulls.
325
345
335
351
311
324
High
„
„
„
„
„
41.3 Max.
41.2
„
41.2
„
40.4
„
40.7
„
41.0
„
8th day
7th „
7th „
Nil.
6th day
7th day
9th day
9th day
9th day
Nil.
8th day
7th day
Recovery.
Died on
12th day.
Died on
11th day.
Recovery.
Died on
9th day.
Died on
10th day.
Dose per 600 lbs.
body weight.
10 c.c. ditto.
10 c.c. ditto.
5 c.c. ditto.
5 c.c. ditto.
2 c.c. ditto.
2 c.c. ditto.
SERUM PREPARATION-EXPERIMENT NO. 3-D.
Plains bulls.—Defibrinated V. B. Two injections at posi-
tive phases.
This serum was prepared on similar lines to serum No. 1-D,
with the exception that defibrinated blood was used in place
of a mixture of blood and Pot. Cit.
Two bulls received a dose of 2 c.c. per 600 lbs. (Plains),
both had a severe attack, one died and one recovered ; two at
5 c.c., both had a severe attack, one died and one recovered.
Two at 10 c.c., one had a severe attack and the other a slight,
both recovered.
The protective dose of this serum was about 12 c.c. per
600 lbs. body weight (Plains).