103
HORSE No. XIII.
Direct inoculation into jugular vein of rat's blood containing the hæmatozoon, followed in twenty-one days by
re-inoculation subcutaneously. Results negative.
Seventy-six days after second injection, inoculation with Horse-Surra blood. Period of incubation 8 days. Death
on the 36th day of disease.
A Bay, entire pony, lame, but strong and in good health.
January 22nd, 1891.—At 3 P.M. 0.6 c.c. of blood containing numerous hæmatozoa, taken from the heart of a rat
(Mus decumanus) was injected directly into the jugular vein.
February 12th, 1891.— As no signs of the Infusorian had been discovered in the blood up to date, the animal was
subjected to re-inoculation, and for this purpose 1.0 c.c. of blood containing numerous hæmatozoa, and from a similar
source to the former, was subcutaneously injected over the right scapular region. The usual symptoms followed at the
seat of inoculation ; the tumour on the 24th measured 1.5"×1" and a clear straw-coloured fluid exuded from it, but
no hæmatozoa were discovered either in this fluid or in the blood. At a later date, the swelling subsided and finally
disappeared.
As up to the 29th April, seventy-six days after the last operation, no symptoms of disease had been induced with
rat's blood containing the Infusorian, it was determined to inoculate with Horse-Surra blood, in order to test whether
the animal had acquired any immunity from the previous injections. Consequently, at 9 A.M., the animal was subcu-
taneously inoculated with 1.0 c.c. of blood in which no hæmatozoa were discovered, it having been drawn during an
intermission.
5th May.—A circular raised swelling was observed at the seat of inoculation 1.5" in diameter, involving the skin and
subcutaneous tissues only.
6th.—The swelling had increased in size and was tense and tender on manipulation.
7th.—The swelling had lost the tension and tenderness, and felt soft and doughy under the fingers. The hæmatozoa
were found to be few in the blood, but active and well developed.
8th.—Temperature A.M. 37.6°C. P.M. 39.7°C. Hæmatozoa numerous.
9th.—
„
„
39.4°C. „ 40.3°C.
„
very numerous.
10th.—Urticarial eruption over the posterior and inner sides of the thighs and the near shoulder. This faded in
the evening.
Temperature A.M. 39.9° C. Hæmatozoa very numerous. Temperature 5 P.M. 39.4°C.
11th.—Temp. A.M. 39°C. Hæmatozoa numerous. Temp. 5 P.M. 40.5°C.
12th.—Swelling at seat of inoculation now measures ½" in diameter.
Temperature A.M. 40.0°C, P.M. 40.3°C.
Hæmatozoa absent.
13th.—
14th.—
l5th.—
16th.—
Temperature
„
„
A.M.
„
„
40.1°C.
38.5°C.
39.7°C.
P.M.
„
„
38.8°C.
40.1°C.
39.4°C.
Hæmatozoa absent.
„
„
numerous.
numerous.
Some œdema of the under-surface of the abdomen.
Temp. A.M. 38.9°C. P.M. 38.6°C. Hæmatozoa numerous.
17th.—
18th.—
19th.—
20th.—
Temp. A.M.
„
„
„
38.2°C.
39.8°C.
37.8°C.
39.0°C.
„
„
„
„
39.7°C.
39.9°C.
39.4°C.
39.2°C.
„
„
„
„
„
„
„
„
21st.—Urticarial eruption involving the neck, fore-limbs, shoulders, and abdomen only. Slight clear discharge
from right nostril, and lachrymation. Temperature A.M. 38.5° C. P.M. 39.5° C. Hæmatozoa numerous.
22nd.—Eruption gradually fading, appetite impaired. Temperature A.M. 39.3° C. P. M. 39.6° C. Hæmatozoa
numerous.
23rd.—Animal dull, urticarial eruption more marked ; appetite remains poor. Temperature A.M. 39.5°C. P.M.
39.6° C. Hæmatozoa absent.
24th.—Temperature A.M. 38.0° C. P.M. 38.9°C. Hæmatozoa absent.
25th.—A muco-purulent discharge from both nostrils.
26th.—
27th.—
28th.—
29th.—
30th.—
31st.—
Temp.
„
„
„
„
„
A.M. 38.5°C.
38.7°C.
39.5°C.
38.3°C.
38.5°C.
39.2°C.
P.M
„
„
„
„
„
38.9°C.
39.7°C.
39.2°C.
39.3°C.
39.3°C.
39.4°C.
Hæmatozoa
„
„
„
„
„
absent.
few.
few.
few.
numerous.
numerous.
Œdema under abdomen has increased in extent.
Temp.
1st June.—
2nd.—
3rd.—
4th.—
„
„
„
„
A.M. 38.8°C.
„ 39.l°C.
„ 37.3°C.
„ 38.2°C.
„ 38.l°C.
P.M.
„
„
„
„
39.6°C.
38.l°C.
38.7°C.
38.5°C.
38.6°C.
Hæmatozoa
„
„
„
„
numerous.
very numerous.
absent.
„
few
5th.—Left sub-maxillary gland considerably enlarged and hard ; it occupies half the space between the rami,
but is not tender on manipulation. Temperature A.M. 39.1°C. P. M. 40.4°C. Hæmatozoa numerous.
6th.—There is a considerable muco-purulent discharge from the right nostril.
Temperature A.M. 39.9°C. P.M. 39.6°C. Hæmatozoa numerous.