100
Date.
1891.
October
5
„ 6
„ 7
„ 8
„ 9
„ 10
„ 11
„ 12
„ 13
„ 14
„ 15
„ 16
„ 17
„ 18
„ 19
„
20
„ 21
„ 22
„ 23
„ 24
„ 25
„ 26
„ 27
„ 28
„ 29
„ 30
Temperature °C.
A.M.
36.1
36.3
36.2
36.1
37.2
36.1
36.6
39.1
36.2
36.0
37.0
35.7
36.4
35.5
38.3
37.8
36.2
36.1
36.8
36.8
36.0
36.6
36.3
36.4
36.7
36.3
1 P.M.
37.2
37.6
38.0
38.0
38.7
38.5
38.7
39.8
38.0
37.1
38.1
38.2
37.3
37.7
38.8
38.3
38.1
37.3
37.7
37.4
37.3
37.8
37.7
37.8
37.5
37.4
5 P.M.
36.8
38.0
37.7
37.8
38.0
37.7
39.4
38.4
39.1
37.3
37.5
39.4
38.0
38.0
38.6
38.5
38.4
38.4
37.8
38.2
37.4
38.0
37.8
38.0
37.5
38.2
Hæmatozoa
in Blood.
Absent ...
„
„
„
„
„
„
„.
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
REMARKS.
There is no swelling or œdema at the seat of inoculation.
„ „ „
„ „ „
There is a small tumour about the size of a walnut hard to the touch
at the seat of inocnlation ; it is very tender on manipulation,
There are two pale red-coloured ecchymoses on the membranes of
the right eye.
Condition unchanged.
„ „
„ „
„ „
„ „
There are two pale-coloured ecchymoses involving the conjunctiva of
the right eye. The tumour at the seat of inoculation has decreased
during the night, and if any hæmatozoa are to be found in the blood
as the result of the inoculation with rat's blood, they ought to be
discovered on microscopical examination this morning.
The general condition of the animal is unchanged.
There is a little thickening under the skin in the subcutaneous tissue
at the seat of inoculation, and there are many ecchymoses on the
conjunctivæ.
Condition unchanged.
„ „
„ „
The animal is weak ; there is some swelling and œdema of the hind
legs and sheath, and several fresh ecchymoses involving the left
membrana nictitans.
Condition unchanged.
„ „
„ „
There are several ecchymoses on the right conjunctiva.
Condition unchanged.
The ecchymoses are extensive on the right conjunctiva.
Condition unchanged.
The swelling and œdema has extended forward along the abdomen,
and has now appeared between the fore-limbs.
Condition unchanged. This animal's temperature was carefully taken
three times, and the blood examined once daily for a further period
of twenty-eight days, but no hæmatozoa were observed in the blood,
the temperature remaining normal or sub-normal. On the 27th
November, the animal became much weaker although the appetite
continued ravenous. Fell at 12-30 P.M. Death occurred at 4-15 P.M.
Autopsy.—45 minutes after death. Body emaciated.
Thorax.—There is no fluid in the pleural cavities, and there are no adhesions of the pleuræ.
Lungs.—The apices and anterior portions of the organs for a distance of six inches are consolidated, and dark-red
in colour ; the remaining portion of each lung is free from disease, and fully distended with air.
Heart.—Weighs 4 lbs. 1 oz. Externally the base is the seat of a considerable amount of sherry-coloured exudation,
but internally the organ presents a healthy appearance.
Liver.—Weighs 5 lbs. 10 ozs. and is small, presents no pathological changes.
Spleen.—Weighs 1 lb. 10 ozs., small, firm, numerous small circular patches on the capsule.
Stomach.—Distended.
On section contains a large amount of semi-digested food. The cuticular portion of the
organ is healthy in appearance ; the villous portion presents a spiroptera tumour, the size of a large walnut.
Kidneys.—Right weighs 1 lb., Left, 13 ozs. The capsules strip easily, and the organs present a healthy
appearance.
Remarks.—This animal was inoculated subcutaneously on 2nd October 1891 with 1.0 c.c. of blood, hæmatozoa
swarming, taken from the heart of a rat (Mus decumanus) immediately after death. Six days later, there was a small
tumour, the size of a walnut, at the seat of inoculation, and a few petechiæ on the conjunctival membrane of the right
eye. Eight days later, the tumour had disappeared, and by the 18th day there was some swelling and œdema of the
extremities and sheath, which later extended forward between the fore-limbs. The animal continued in this condition
for more than three weeks, when it succumbed. The chief point of interest at the autopsy was the bi-lateral and sym¬
metrical circumscribed patches or consolidation situated at the anterior portions of both lungs, for similar patches have
been described in other cases as per successful inoculation.