124
Date.
1894.
January
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
14...
15...
16...
17...
18...
19...
20...
21...
22...
23...
24...
25...
26...
27...
28...
29...
Temperature ° C.
A.M.
37.7
37.7
38.0
38.1
38.1
38.1
38.1
37.1
37.7
37.5
38.0
37.8
38.1
37.2
38.1
37.7
1 P.M.
38.2
38.0
38.0
38.2
38.3
38.2
38.2
38.1
38.3
37.7
37.6
38.5
37.6
37.8
37.8
...
3 P.M.
38.4
38.6
39.2
39.1
38.7
38.7
38.6
38.8
39.0
39.4
38.8
30.1
39.3
39.7
39.0
...
Pulse.
A.M.
44
48
52
56
48
56
44
48
56
56
56
56
56
48
56
40
P.M.
52
56
68
52
56
52
56
48
52
60
56
64
52
48
64
...
Respiration.
A.M.
12
12
16
16
12
16
12
12
12
12
16
12
12
12
16
24
P. M.
16
16
16
16
12
46
12
20
12
16
16
16
20
20
16
...
Hæmatozoa
in Blood.
Absent.
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„
„
„
„
„
„
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Urine.
Colour.
Clear.
„
Turbid.
„
Clear.
„
„
„
„
„
„
„
„
„
„
„
Sp. Gr.
1,027
1,025
1,026
1,048
1,041
1,040
1,053
1,028
1,030
1,036
1,034
1,033
1,042
1,040
1,022
1,026
Reaction.
Alkaline.
„
„
„
„
„
„
„
„
„
„
„
„
„
„
„
Albumen.
Nil.
„
„
„
„
„
„
„
„
„
„
Trace.
Nil.
Trace.
Nil.
„
Hippuric
Acid.
Trace.
1/12
1/18
Trace.
1/18
1/13
1/6
1/12
1/20
1/18
1/18
Trace.
Nil
Trace.
„
Nil.
Remarks.—The chief points to draw attention to in this case are :—
(i) This is the first case recorded of any animal of the bovine species having been successfully inoculated
with the hæmatozoon of the rat (Mus docamanus).
(ii) The amount of swelling and œdema, which followed inoculation at and around the point of injection,
which finally disappeared on the 14th day. Also the high temperature 41° C. (105.8° F.) registered on the morning
of the 8th day.
(iii) The protracted period of incubation of the disease, whether we take it as terminating on the first
appearance of the urticarial eruption on the 44th day, as previous experience points to the hæmatozoon being the
cause of the manifestation in the skin, or the 76th day, when the organism was found on microscopical examina-
tion of the blood. In either case the length of the latent period has only once been exceeded, viz., in Horse
No. LXII, inoculated with soiled rat's blood, and which occupied 63 days.
(iv) The discovery of the ' Trypanosoma' in the blood on the 76th day after inoculation, when a long and
active specimen of the organism was observed, the dorsal membrane in which was particularly well developed
and its complete absence from all other blood examined daily during a further period of 121 days.
(v) The marked evening exacerbations and morning remissions of temperature, which occurred on the 55th,
56th and 59th days of the disease.
(vi) The loss of flesh during the four months, which immediately followed inoculation.
(vii) Re-inoculation of the animal with virulent soiled blood 197 days after the former injection, followed
by high fever, but total absence of swelling at the seat of inoculation. Appearance of the Surra organism in the
blood for a period of 48 hours, 80 days later, and re-appearance for 24 hours on the 158th day, but total absence
during a further period of 195 days.
MONKEY No. II.
Date.
1893.
March 23 ...
„
24 ...
„
25 ...
„ 26 ...
„ 27 ..
„ 28 ..
Day of
Disease.
Temperature °C.
A.M.
38.2
1 P.M.
38.6
5 P.M.
38.9
Pulse.
A.M.
60
P.M.
64
Respiration.
A.M.
40
P.M.
48
Hæmatozoa
in Blood.
Healthy.
5 P.M., 23rd.—Inoculated subcutaneously in the right inguinal region with
1.5 c.c. of blood, containing numerous hæmatozoa taken from the hearts of
two rats (Mus decumanus) immediately after death.
...
38.8
39.0
38.8
48
60
40
48
Absent.
24th.—There is slight thickening at the seat of inoculation.
...
38.2
39.0 39.2
60
64
42
48 „
25th.—A small tumour the size of a marble, tender on manipulation, is present
at the seat of inoculation.
1
38.4
38.6
39.2
48
60
40
48
Few.
26th.—The tumour has almost disappeared together with the tenderness.
2
3
38.2
38.5
38.6
39.0
38.8
39.4
48
60
68
68
48
48
56
48
Absent.
„
28th.—The tumour has entirely disappeared.
REMARKS.