52
T ABLE VIII (a)---cont.
Date.
Experiment No. 1.
N u m b e r .
S e a c h .
D u l c i t . A d o n i t .
I n u l i n e . V a n d P . M o b i l i t y .
Bacillus.
Remarks.
11th Sept. 1908. Sample 13. End of day + in 5 cc. of
water.
L
1
2
3
4
5
7 6
8
9
10
11
12
+
+
+
+
+ \
+
+
+
+
—
—
—
_
—
—
—
+
+
+
+
+ +
+
+
+
-
—
—
—
—
_
—
-
-
—
—
—
—
—
—
_
—
+
—
--
--
--
—
—
—
—
_
—
+
—
N o. 101
...
Do.
...
Do.
...
Do.
...
Do.
...
Do.
Do
...
Do.
...
Do.
...
Do.
...
Lactis ÆA r o g e n e s
No. 101
...
VI
V
VI
VI
VI
VI
VI
VI
VI
VI
VI
VI U
Temp. 30° C.
12th
do.
14th
do.
15th
do.
16th
do.
17th
do.
18th
do.
14. End of day + in 5 cc. of
water.
„
15.
E n d o f E n d o f d a y +
i n
5 c c . , . - {
16. End of day + in 5 cc. of
water.
17.
,,
+
,,
„
18. „
„
s t e r i l c
19. ,,
,,
,,
• • •
...
1
2
3
4
5
6
S
9
10
11
12
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...
• • •
...
+ +
+
+
+
+
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.
+
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:
...
.
...
+
+
++
+
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. ...
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...
...
•••
—
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--
7 - -
—
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...
...
.
.....
--
--
--
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—
=
—
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i ...
1
•••
......
Coscoroba ...
No. 101
...
Do.
Do.
Do.
Do
Do.
Do.
Do.
Do.
Do.
......
......
......
......
VI
V1
VI
VI
VI
VI
vi
VI
VI
VI
VI
VI
...
...
. . .
...
Temp. 30° C.
Teinp. 31° C.
J
......
••••••
......
......
Experiment No. 2.—This experiment is exactly on a par with the first, but the
variety of organisms present was not anything like so great as in Experiment 1 ;
consequently the details of each analysis will not be given. The emulsion was placed
in the sun on the 9th of September and by the 14th no fwcal bacilli were found in
5 cc. of the emulsion.
The last organism shown to be present was bacillus grunthal ; coli communis was
not found after the mixture had been exposed, for a period of one hour. In this
particular sample of human fœces coli communis were rare : lactose aerogenes and many
others were entirely absent, the common organisms obtained being bacillus coscoroba,
bacillus grunthal, and one unnamed bacillus No. 67.
Experiment No. 3—Cowdung.—Precisely similar mixtures of cowdung and
water were placed in the sun. The one exposed on the 2nd of September contained no
f w c a l b a c i l l i i n 5 c c . o f t h e e m u l s i o n b y t h e e v e n i n g o f t h e s a m e d a y . F i v e h o u r l y
samples were taken during the day, subsequent samples contained no lactose fermenters.
The number of varieties of organisms present in this sample was very limited,
those present being almost entirely bacillus grunthal, with a very few coscoroba.
Experiment No. 4.—A mixture of cowdung was placed out on the 9th. Sam-
ples taken, one hour, 3 hours after exposure, and on the following day all showed no
f œ c a l b a c i l l i i n 5 c c . T h e o r g a n i s m s h a d e n t i r e l y d i s a p p e a r e d i n l e s s t h a n o n e h o u r .
Experiment No. 5. —The usual mixture of cowdung and water was exposed on the
11th of September. Samples were taken 1 hour, 2 hours and 4 hours afterwards.
The samples taken 1 after hour contained fœcal bacilli in 5 cc. yielding practically a pure
culture of bacillus grunthal. Samples taken 2 and 4 hours afterwards contained no
f c a l b a c i l l i i n t h i s a m o u n t . T h e m i x t u r e h o w e v e r w a s v e r y p o o r i n v a r i e t i e s
present ; out of 12 colonies, 11 were bacillus grunthal, and 1 bacillus cloacæ.
This concludes what may be called the preliminary series of experiments. It
would be unsafe to draw any important conclusions from these, but it appears