NationalLibraryOfScotland/medical-history-of-british-india huggingface dataset:
75
CHAPTER XI.
THE CLASSIFICATION OF FŒCAL ORGANISMS ACCORDING TO THEIR POWER
TO RESIST SUNLIGHT.
This subject is the logical conclusion of the foregoing Chapters VIII, IX
and X ; but use is made of all the experience gained in the previous parts of this
report, particularly that in Chapter IV on the bacteriology of polluted earths.
Before giving the details of the proposed classification of organisms, according to
their power of resistance to the action of sunlight, it is necessary to say a few words
about each, and, as briefly as possible, to record any facts ascertained as to the natural
characteristics and distribution in nature, of the various lactose fermenting bacilli
met with in fœces of man and animals.
The characteristic fermentation reactions, of all the organisms met with, are found
in the table No. XI (a), published by MacConkey, but concerning these reactions we
propose to say little or nothing. The organisms marked with asterisks are those
added by ourselves, and must be taken with certain amount of reserve, for up to the
time of going to press, we have not completed the study of all the necessary
fermentation reactions of these bacilli. The numbers applied to these bacilli also must
be looked upon as provisional only.
TABLE XI (a).
After MacConkey (1909).
0
ti
c
f
o
on
s
u
, E
t
k
f
0
No.
se
o
e s
Ft lci o n i
li
a
r
v V
o
i
ta
ty
li
tic
t
c
a
f
e
u
s
o ti at
.
t
si
a
a u
l
S
d
d
ci
Li
A
e
d n i
R
no
I
%
1
+ -
- + -
- + ± - I -
+
+
-
2 B. Acidi lactici (Huppe)
...
... + - - . + - - + - - ' - +
23
+
-
3
4
5
B. Levans
...
...
B. Grunthal
B. Sulcatus gasiformans
B . C a s t e l l u s
B
B. Vesiculosus
•
...
...
•
••
+ - -
4- - -
+ -
-
-
-
+
-
-
-I-
-
-
-
+
- +
I
-1- - -
I
- - -
-I-
14
+
20
+
25
+
+
-
-
6
••• -1- - - - -
+ + - - I - +
.
...
7
.
± - - - - - - + - 1 - 1
•••
•••
-
8 B. Coli mutabilis (Massini)
•••
.••
•••
*9
••• -1-
+ + - ••• I - -
-
...
...
*10
33
-1- - + + - - + -I- - I - +
...
...
-
34 f B. Coli communis
...
'••
1 B. Cavicidze (Brieger) " '
'"
+ - +
"'
-
•• '
- -
•-•
+
•••
+ -
••• •••
I -
••• +
28
•
•••
+
...
-
...
35 B. Schwfferi ...
...
...
-I- - + - - - ± - - - +
22
+
-
36
+ - -1-- - - - - + - -1- ±
...
+
-
*37
- • + - -1- - - + - - -
•••
...
...
,..
*38
••• + - -1- + - + + -
*3 9
..
T - + - + -
+ -
•••
-
••
• •
- .-
65
66 66
B. Ox ytocus pernioiosns
...
...
+ +
-1- -1- -I-
+
+
+
- - --t-
+
-
— —
+
—
+
26
+
...
+
-
67
..
... -1- ± + + - + 7 — — — +
...
+
+
68 B. Pneumonim (Friedlander) B.
Rhinoscleroma
...
...
... + -1- + + - - - - +
14
+
+
69
- ± + ± - T + -
- + +
70
... +
T
+ -1- -
-
-
-
±
-
-
+
-
+
+
...
...
-
72 B. Neapolitanus
...
...
:::
+ + - - - + - _ _ +
+
22
-I-
_
73
74
... ... T. t ± - - +
t - - ± _ + ..-
-
±
•••
-
75
... + + +.
-
- - - - +
..•
...
1
97
98
99
...
...
+
t -/-
+
:
+
t
+
T
±
--h
±
-
- -
- _ ± _ + + ...
•••
+
+
+
+
100
- + -i- - + - - 4- ± - ±
-
-
-
101
- +
-
+
+ - - ± - - -1-
..-
+
+
102
... - + - + - + - -1- - -
•••
•••
•••
•
85
Analyses characteristic of certain natural conditions in water.
(1) SAMPLE IV.—FROM A FOUNTAIN IN FRONT OF
CHOULTRY NEAR RAILWAY STATION—I MILE
FROM SERVICE RESERVOIR.—
C o l o n y n u m b e r .
S a c c h a r o s e
. D u l c i t .
A d o n i t .
I n u l i n .
V o g e s a n d P r o s k .
M o t i l i t y
.
G r o u p .
Bacillus .
_
(a) Total colonies on agar
at 37°—110.
+
—
(b) Bile
salt
lactose,
medium—
1 tube 20 cc. of water
1
taken.
2 tubes 10 cc. of
2
..,
water taken.
4 tubes 5 cc. of water
3
1
taken.
5 tubes 1 cc. of water
1
4
taken.
5 tubes '1 cc. of water
...
5
taken
5 tubes •01 cc. of
„ ,
5
water taken.
(a) Fœcal bacilli in 5 cc. and upwards.
(d) Sporogenes milk test —
in 20 cc.
1
.
1
—
+
—
—
—
—
II
I
3
— + — — — —
II
4
+
—
—
—
—
—
IV
5
+
+
—
—
—
—
III.
6
+
—
—
—
—
—
IV
7
+ + I– – – –
III
8
+
+
—
—
—
—
III
9
+
+
—
—
III
1 0 + +
-
—
—
—
III
B. Coli communis.*
. ,
B. Grunthal.*
B. Coli communis.*
B. Coscoroba.
B. Neapolitanus.
B. Coscoroba.
B. Neapolitanus.
Do.
Do.
Do.
(2) SAMPLE II.— A SHALLOW WELL ON THE BANK
OF THE KALYANI RIVER WEST OF THE HEAD
WORKS--AT A DISTANCE OF 320 FEET FROM
THE 1ST UNFILTERED WATER-
(a) Total colonies on agar
at 37°—3,150.
+
_
(b) Bile , salt
lactose,
medium—
1 tube 20 cc. of water
1
...
taken.
2 tubes 10 cc. of water
1
1
.
taken.
4 tubes 5 cc. of water
...
4
taken.
5 tubes 1 cc. of water
...
5
taken.
5 tubes • 1 cc. of water
...
5
taken.
5 tubes • 01 cc. of
5
water taken.
•
1
—
—
—
—
—
I
2
+
—
—
—
+
—
IV
3
+
—
—
—
+
+
IV
4
—
+
—
—
—
—
II
5
—
—
—
—
—
+
I
6
—
+
— I—
—
—
Il
7
—
+
—
—
—
—
II
8
—
+
—
+
II
9
+
—
+
+
IV
10
—
—
+
I
,
B. Grunthal.*
B. Cloacæ.*
Do.
B. Coli communis.'
B. Grunthal. .
B. Coli communis.*
Do.
Do.
B. Cloacæ.
B. Grunthal.
(c) Fœcal bacilli in 20 cc. and upwards.
(d) Sporogenes milk test —
in 20 cc.
(3) SAMPLE IV.—BOOSIKAL CHANNEL—
(a) Total colonies on agar
at 37º-170.
+
-
(b) Bile
salt
lactose;
medium-
1 tube 20 cc. of water
1
.
taken.
2 tubes 10 cc. of
2
water tak en .
4 tubes 5 cc. of water
4
...
taken..
5 tubes 1 cc. of water
5
...
taken.
5 tubes . 1 cc, of water
1
4
taken.
5 tubes 01 cc. of
...
5
water taken.
1
—
+
—
—
—
—
II
I
3
—
+
—
—
—
—
II
4
+
+
—
—
—
+
III
5
—
—
—
—
—
+
I
6
+
—
—
—
—
+
IV
7
+
—
—
—
—
+
IV
8
—
—
—
—
+
I
9
—
+
—
—
—
—
II
10
+
—
—
—
—
+
IV
B. Coli communis.*
B. Grunthal.*
B. Coli communis.*
B. Neapolitanus.
B. Grunthal.
B. Coscoroba.*
Do.
B. Grunthal.
B. Coli communis, etc.*
B. Coscoroba,
(a) Fœcal bacilli in 1 cc. and upwards.
(d) Sporogenes milk test
— in 20 cc.
(1) A polluted pipe water due to defect in pipes — a bad mixture.
(2) A bad well water — note fewness of faecal organisms but presence of class 1.
(3) A bad mixture in a surface channel.
22
•
Analyses characteristic of certain natural conditions in water—cont.
4) SAMPLE I.—STORAGE RESERVOIR — UNFIL-
TERED WATER—
C o l o n y n u m b e r .
S a c c h a r o s e .
D u l c i t .
A d o n i t .
I n u l i n .
V o g e s a n d P r o s k .
M o t i l i t y .
G r o u p .
Bacillus.
No. 73.
No. 67.
No. 73.
B. Cloacm.*
No. 73.
No. £7.
No. 73.
(a) Total colonies on agar
at 37°—630.
(b) Bile
salt
lactose
medium—
1 tube. 20 cc. of water
taken.
2 tubes 10 cc. of
water taken.
4 tubes 5 cc. of water
taken.
5 tubes 1 cc. of oater
taken.
5 tubes • 1 cc. of water
taken.
5 tubes • 01 cc. of
water taken.
'4.
_
C C
1
2
3
4
6
6
7
+
+
+
I +
+
+
+
+
+
+
-
+
+
+
-
+
-
-
-
+
-
-
-
-
-
-
—
+
+
+
+
i +
+
-
-
-
-
-
-
–
III
III
III
IV
1I1
III
III
' 1
2
4
5
1
...
...
...
...
4
5
(c) Fœcal bacilli in 1 cc. and upwards.
8
(d) Sporogenes milk test —
in 20 cc.
9
10
j
+
1
+
1 +
+
+
-
+
-
+
-
-
-
i
+
+
+
-
-
-
III
III
IV
No. 67.
Do.
B. Cloacæ.*
(5) SAMPLE II.—FIRST TAP AFTER FILTRATION — 1
2
+
+
-
4
—
—
-
-
+
-
—
III
-
II
No. 73.
B. Coli communis.
Do.
13. Grnuthal.*
B. Coscoroba.
B. Grunthal *
B. Coli communis.'*
No. 75.
No. 73.
B. Coli communis, etc.*
(a) Total colonies on agar
_
at 37°—170.
(5) Bile
salt
lactose,
medium
—
1 tube 20 cc. of water
taken.
2 tubes 10 cc. of
water taken.
4 tubes 5 cc. of water
taken.
5 tubes 1 cc. of water
taken.
5 tubes 1 cc. of water
taken.
5 tubes .01 cc. of
water taken.
+
-
3
- 5"
7
8
9
10
-
+
-
+
+
-
+
-
+
+
+
+
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
+
-
-
-
-
-
-
II
I
IV
I
II
III
III
II
1
2
4
1
...
...
...
...
...
4
5
5
(c) Fœcal bacilli in 5 cc. and upwards.
(d) Sporogenes milk test — in 20 cc.
(6) SAMPLE
III.—EASTERNMOST
TAP
NEAR
B E A C H —
1
2
+
-
+
-
-
-
...
+
-
-
+
+
+
+
+
+
+
+
-
+
-
-
-
...
-
-
-
-
-
-
-
-
-
...
-
-
-
+
-
+
-
-
...
+
-
-
-
-
-
-
-
-
...
-
-
-
III
II
III
II
II
II
...
III
II
II
No. 67.
B. Coli communis.*
No. 67.
B. Coli communis.*
Do.
Do.
B. Fœcalis alkaligenes
(P).
No. 73.
B. Coli communis.*
Do.
(a) Total colonies on agar
at 37°—30.
(5) Bile
salt
lactose,
medium—
1 tube 20 cc. of water
taken.
2 tubes 10 cc. of
water taken.
4 tubes. .5 c c . o fw
a t e r
taken.
5 tubes 1 cc. of water
taken.
5 tu
a
bes .1 cc. of water
t ken.
5 tubes . 01 cc. o
water taken.
+
-
3
4
5
6
7
8
9
10
1
..
2
...
4
...
5. • •
1
4
...
5
(c) Fœcal bacilli in 1 cc. and upwards.
(a) Sporogenes milk test —
in 20 cc.
(4) Fairly good lake water—note, bacilli belong to more resistant end of class II and class III•
(5) Same water polluted with mixture of sub-soil water through leaky filters.
(6) Typical bad mixture same series as 4 and 5 — note, presence still of bacilli Nos. 67 and
73, occurring in the lake.
87
. Analyses characteristic of certain natural conditions in water—cont.
,
(7) SAMPLE II —DODABETTA LOWER RESERVOIR —
(a) Total colonies on agar
at 370-5,040.
+
—
(b) Bile
salt
lactose,
m e d i u m — .
1 tube 20 cc. of water
1
...
taken.
2 tubes ].0 cc. of water
2
...
taken.
4 tubes 5 cc. of water
4
...
taken.
5 tubes 1 cc. of water
5
...
taken.
5 tubes 1 cc. of water
...
5
taken.
5 tubes '01 cc. of
...
5
water taken.
(s) Faecal bacilli in 1 cc. and upwards.
(d) Sporogenes milk test —
in 20 cc.
C o l o n y N u m b e r . S a c c h a r o s e . D u l o i t . A d o n i t . I n u l i n . V o g e s a n d p r o s k . M o t i l i t y . G r o u p .
o
Bacillus.
tz
;
+ • —
+
—
+
IV B. Lactic wrogenes or
capsulatus.
2
+
—
+
—
+
—
I V
Do.
3
+
+
+
—
+
—
III No. 67.
4
+
+
—
+
—
III
Do.
5
+
—
+
—
+
—
IV B. Lactis ærogenes or
capsulatus.
6
+
—
+
—
+
—
IV
Do.
7
+
+
—
—
—
—
III B. Neapolitanus.
8
+
—
—
—
+
—
1V B Cloacæ
9
+
—
—
—
+
—
IV
Do.
10
+
—
—
—
+
—
IV
Do.
—
(8) SAMPLE I.—CHANNEL AT WEST END OF RED
HLLLS LAKE—
(a) Total colonies on agar
at 37°-78.
+
—
(b) Bile
salt
glucose,
medium —
1 tube 20 cc. of water
1
taken.
2 tubes 10 cc. of
2
...
water taken.
4 tubes 5 cc. of water
4
...
taken.
5 tubes 1 cc. of water
5
...
taken.
5 tubes 1 cc. of water
3
2
taken.
5 tubes 01 cc. of
...
5
water taken.
(c) Faecal bacilli in 1 cc.
(d) Sporogenes milk test doubtful in 20 cc.
1
+
—
—
—
+
+
IV
B æ.
2
+
—
—
—
+
+
IV
Do.
3
+ I —
—
—
+
+
IV
Do.
4
+
—
—
—
+
+
IV
Do.
5
+
—
—
—
+
—
IV
Do.*
6
+
—
—
—
+
+
IV
Do.
7
+
—
—
—
+
+
IV
Do.
8
+
—
—
—
+
+
IV
Do.
9
—
—
+
+
IV
Do.
10
+
—
—
—
+
+
IV
Do.
11
+
—
—
—
+
+
IV
Do.
12
+
—
—
—
+
+
IV
Do.
13
+
—
—
—
+
—
IV
Do.*
14
+
—
—
—
+
—
IV
Do.*
15
+
—
—
—
+
—
IV
Do.*
(9) SAMPLE II.—DISTRIBUTARY SYSTEM—A TAP
NEAR CATHEDRAL—
(a) Total colonies on agar
at 37°-630.
+
_
(b) Bile • salt
glucose,
medium-
1 tube 20 cc. of water
1
...
taken.
2 tubes 10 cc. of
2
...
water taken.
4 tubes 5cc. of water
4
...
taken.
5 tubes 1 cc. of water
3
taken
5 tubes 1 cc. of water...
taken.
5 tubes 01 cc. of
...
5
water taken.
(c) Faecal bacilli in 1 cc. and upwards.
(d) Sporogenes milk test —
in 20 cc.
B. Grunthal.*
2
I
Do.
3
. I
Do.
4
+
—
—
—
+
—
IV B. Cloacae.
I B. Grunthal.*
I
Do.
(7) Fair lake water ; note, mixture mostly cloacæ and lactis ærogenes, in classes II and III.
(8) A lake very low, otherwise not very objectionable, lactose formenters too many numerically.
'Cholera broke out in this town 3 weeks later.
(9) Same water as (8) with pollution added since water left lake.
88
Analyses characteristic of certain natural conditions in water—cont.
(10) SAMPLE VI.—WATER OF THE RED HILLS
LAKE —
(a) Total colonies on agar
at 37°-
1 6 0 .
+ —
(b) Bile
salt
lactose,
medium—
1 tube 20 cc. of water
1
...
taken.
2 tubes 10 cc. of
2
...
water taken.
4 tubes 5 cc. of water
4
...
taken.
5 tubes 1 ce. of water
...
taken.
5 tubes .1 cc. of water
...
5
.
taken.
5 tubes • 01 cc. of
...
5
water taken.
(c) Fœcal bacilli in 5 cc. and upwards.
(d) Sporogenes milk test +
in 20 cc.
C o l o n y
N u m b e r
S a c c h a r o s e .
D u l c i t .
A d o n i t .
I n u l i n .
V o g e s
a n d
p r o s k .
M o t i l i t y .
G r o u p
Bacillus.
1 + — + — + —
IV
2+ — + — + —
IV
3+— + — + —
IV
4+ — + —
IV
5+ — + — + —
Iv
6+ — + — + —
IV
7+ — + — + —
IV
8+ — + — + —
IV
9+ — + — +
IV
10+ — — — — +
IV
B. Lactic æogenes.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
B. Coscoroba.
(11) SAMPLE I.—SAMALROTA CANAL—
(a) Total colonies on agar
at 37°—1,890.
+
—
(b) Bile
salt
lactose,
medium—
1
tube 20 cc. of water
1
...
taken.
2 tubes 10 cc. of
2
...
water taken.
4 tubes 5 cc. of water
3
taken.
5 tubes 1 cc. of water
3
2
taken.
5 tubes • 1 cc. of water
...
5
taken..
.
5 tubes .01 cc. of .. 5
water taken.
(c) Fœcal bacilli in 1 cc. and upwards.
(d) Sporogenes milk test —
in 20 cc.
1 + — + — + — I V
2 + — + — + — I V
3 + — + — + — I V
4 + — + — + — I V5
+ — + — + — I V6
+ — + — + — I V7
+ — + — + — I V8
+ — + — + — I V
9 + — + — + — I V 1 0 + — — — — + I V
B. Grunthal.*
Do.
Do.
Do.
D o .
Do.
Do.
Do.
Do.
Do.
(12) SAMPLE II.—STORAGE TANK—UNFILTERED
WATER—
(a) Total colonies on agar
at 37°—170.
+
—
(b) Bile
salt
lactose,
medium—
I tube 20 cc. of water
1
...
taken.
2 tubes 10 cc. of
2
...
water taken.
4 tubes 5 cc. of water
...
4
taken.
5 tubes 1 cc. of water
...
5
taken
.
5 tubes • 1 co. of water
...
5
taken..
5 tubes • 01 cc. of
...
5
water taken.
(a) Fœcal bacilli in 10 cc. and upwards.
(d) Sporogenes milk test —
in 20 cc.
1— — — — — — I
2— — — — — — I
3 — — — — — — I
4 — — — — — + I
5 — — — — — — I
6— — — — — — I
7— — — — — — I
8 — — — — — — I
9— — — — — — I
10— — — — — — I
B. Grunthal.*
Do.
Do..
Do.
Do.
Do.
Do.
.
Do.
Do.
Do.
(10) A typical fair quality lake. Water—note, moderate number of fœcal organisms and
preponderance of lactis ærogenes.
(11)A typical well sunned water in river (irrigation canal)—note, improvement between (11)
and (12).
(12) A typical well sunned water in settling tank—note, improvement between (11) and (12).
89
Analyses characteristic of certain natural conditions in water—cont.
(13) SAMPLE I.--FROM THE RESERVOIRAT—
(a) Total colonies on agar
at 37°—69 unfiltered.
+
-
(b) Bile
salt
glucose,
medium—
1 tube 20 cc. of water
1
C o l o n y
n u m b e r .
S a c c h a r o s e .
D u l c i t .
Adonit.
I n u l i n .
V o g e s
a n d
p r o s k .
M o t i l i t y .
G r o u p .
.
Bacillus.
taken.
tubes 10 cc. of
2
...
water taken.
4 tubes 5 cc. of water
4
..
taken.
5 tubes 1 cc. of water
5
...
taken.
5 tubes '1 cc. of water
2
3
taken.
5 tubes '01 cc. of
2
3
water taken.
(c) Fœcal bacilli in 1 cc. and upwards.
(d) Sporogenes milk test doubtful in 20 cc.
1
+
-
-
-
+
- I V
2
+
-
-
-
+
-
IV
3
+
-
-
-
+
-
IV
4
+
-
-
-
+
-
IV
5
+
-
-
-
+
-
IV
6
+ - - - + -
IV
7
+
-
+
-
IV
8
+
- — — — - + - I V
9
+
-
-
-
+
+ IV
10
+
-
-
-
+
+
IV
11
+
-
-
-
+
- IV
12
+
-
-
-
+
-
IV
B. Cloacæ.*
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Do.
Do.*
Do.
(14) SAMPLE. I.— FROM THR RIVER-BED- -
(a) Total colonies on agar
at 37°—Countless (in
1 cc.).
+
-
(b) Bile
salt
glucose,
medium—
1 tube 20 cc. of water
1
...
taken.
2 tubes 10 cc. of
2
...
water taken.
4 tubes 5 cc. of water
t a k e n .
5 tubes 1 cc. of water
5
...
taken.
5 tubes 1 cc. of water
5
...
taken.
5 tubes '01 cc. of
1
4
water taken.
(c) Fœcal bacilli in .1 cc. and upwards.
(d) Sporogenes milk test —
in 20 cc.
1
+ — — — + —
IV
2
+
-
-
-
+
-
I V
3
+ -
-
-
-
-
IV
4
+
-
-
-
-
-
IV
5
+
+
-
+
+
-
III
6
+
-
-
-
-
-
I V
7
+
+ + +
+ —
III
8
—
—
—
— — —
I V
9 + - - - + + I V
10
+
-
-
-
IV
B. Cloacæ.*
Do.
B. Coscoroba.
B. Grunthal.*
No. 69.
B. Coscoroba.
B. Oxytocus Perniciosus..
B. Grunthal.*
Do.
B. Coscoroba.
(15) SAMPLE I.—FROM THE RUNNING WATER OF .
THE VAIGAI RIVER (UNFILTERED WATER)
—
(a) Total colonies on agar
at 37°—Countless.
+
-
(b) Bile
salt lactose,
medium-
1 tube 20 cc. of water
1
...
taken.
2 tubes 10 cc. of
2
...
water taken.
4 tubes 5 cc. of water
4
...
taken.
5 tubes 1 cc. of water
5
...
taken.
5 tubes -1 cc. of water
5
...
taken.
5 tubes '01 cc. of
5
...
water taken.
(c) Fœcal bacilli in • 01 cc. and upwards.
(d) Sporogenes milk test +
in 20 cc.
1 —
+ — — — —
II
2-
+
-
-
-
-
II
3+ — — — —
II
4- + - - - -
II
5
-
+
-
-
-
-
II
6
-
-
-
-
-
II
7 + - - - + - I V
8
+
-
-
-
-
-
I
9
+
+
-
-
-
-
III
10
-
+
-
-
-
-
II
B. Coli communis.*
Do.
Do.
Do.
Do.
Do.
B. Grunthal.*
Do.
B. Neapolitanus.
B. Coli communis, etc.*
(13) Lake—Very low indeed; no rain for 8 months. Organisms rather too numerous.
(14) and (15)Typical bad mixtures in river water ; note, prevalence of class 1 and mixtures of
organisms.
28
90
Analyses characteristic of certain natural conditions in water—cont.
(16) SAMPLE I.—WATER FROM ABOVE THE INFIL-
TRATION GALLERY--UNFILTERED WATER—
(a) Bile salt
lactose,
+
—
C o l o n y
N u m b e r .
S a c c h a r o s e .
D u l c i t .
A d o n i t .
Inulin.
V o g e s
a n d
p r o s k .
M o t i l i t y .
G r o u p .
Bacillus.
1 — — — — — — I
2+— — — — — — IV
3+ +
+ — — —
III
4+— + — + —
IV
5— + — — — —
II
6— + —
— — —
II
7— — — —
— — I
8— + — — — —
II
9+ — —
— — — IV
10+
+— — +—
III
B. Grunthal.*
B. Coscoroba.
B. Pneumoniæ or Rhi-
noscleromatis.
B. Lactis ærogenes or
capsulatus.
B. Coli communis.*
Do.
B. Grunthal.*
B. Coli communis.*
B. Coscoroba.
No. 73.
(16) Typical bad mixtures in river waters ; note prevalence of class I and mixtures of organisms.
94
GROUP A.
TABLE XIII (a ).
River waters.
—
Quarter
ending 30th
September
1908.
Quarter
ending 31st
December
1908.
Quarter
ending
31st
March
1909.
—
Quarter
ending 30th
September
1908.
Quarter
ending 31st
December
1908.
Quarter
ending
31st
March
1909.
1. Natural or unfiltered waters—
No. of times
i sol at ed. %
No. of times
isolated. %
No. of times
isolated. %
B. Cloacæ
..
13 18
7 8.75
5
No. of times
isolated. %
No. of times
isolated. %
No. of times
isolated. % B. Grunthal ..
.. 28 38.9 37 46.25 14
1
Group I .. 32 43.8 43 53.75 22
22 B. Lactis ærogenes
.. 11 15.3
4 5.0
15
1
B. Coscoroba ..
..
5
7.0
4 5.0
19 1
"
II ..
3
4.1
10 12.5
2
2 B. Coli communis
..
3
4.2
7 8.75
1
B. Neapolitanus
..
1
1.4
1 1.25
8
"
III .
7
9.6 12 15.0
25
25 B. Pneumoniæ
.. .
1
1.4
3 3.75 16 1
B. Acidi lactici
..
.
. .
6 7.5
8
" IV .. 31 42.5 15 18.75 51
51 B. Oxytocus perniciosus,
unnamed bacilli.
. .
..
1 1.25
. . . .
No.
6
..
4
5.5 ..
.. ..
. .
" 100
1
1.4 ..
.. 12
" 75
..
4
5.5 _..
..
1
"
67
..
1
1.4
6 7.25 ..
"
69
.. ..
. .
1 1.25 ..
"
39
.. ..
..
2 2.5
..
. .
"
9
. .
. .
. .
. .
. . . .
"
33
.. ..
..
1 1.25
. .
. .
"
39
. .
. .
. .
. .
1
72 100.0 80 100.0 100
B. Cloacæ
..
.. 44 55.7 34 37.7
9 11
2 Filtered Waters—
B. Grunthal ..
.. 11
14.0 12 13.3 20 25
Group I .. 13 16.7 13 13.3 17 21.25 B. Lactis ærogenes
.. 11 14.0
5
5.5 10
1 2
B. Coscoroba ..
.
3
3.8
6
9.6
7
8
" II ..
.
.
14 15.5
9 11.25 B. Coli communis
.. ..
..
4
4.4
5
0
B. Neapolitanus
.. ..
..
1
1 . 1 1 1
"
III ..
7
9.0 17 18.8 13 16.25 B. Pneumoniæ
. .
. .
. .
. .
2 2
B. Acidi Lactici
..
2
2.5 ..
.. ..
. .
"
IV .. 58 74.3 46 51.1 41 51.25 B. Oxytocus Perniciosus,
unnamed bacilli.
..
..
2
2.2
. . . .
No.
6
. ..
..
. .
. . ..
" 100
..
1
1.2
1
1.1
8 10
"
75
..
2
2.5
3
3.3
7 8
"
67
..
5
6.3 10 11.1
9 11
"
69
.
..
..
1
1.1 .
"
39
.. ..
.. 10 11.1 ..
"
9
.. ..
..
1
1.1
1 1
"
99
.. ..
..
..
..
1 1
79 100.0 90 100 0 80 10
B. Cloacæ. .
.. 31 22 3 64 33.3 22
3. Water that has been through pipes—
B. Grunthal .
. 29 20 9 48 24
29
Group I .. 38 27.3 61 31.7 50 29.5 B. Lactis ærogenes
.. 17 12.2 23 12
17
B. Coscoroba ..
..
5
3.6 23 12
42
"
II .. 15 10.8
2
1 . 0
6
3.5 B. Coli communis
.. 14 10.1
2
1.1
6
B. Neapolitanus
..
4
2.9
3
1.5
1
"
III .. 25 18.0 14
7.3 23 13.4 B. Pneumoniæ
.. 11
8.0 ..
. . 10
B. Acidi Lactici
..
7
5.0 10
5.2 18
"
IV .. 61 43.9 115 59.8 93 54.1 B. Oxytocus Perniciosus,
unnamed bacilli.
. .
. .
. .
. .
. . . .
No.
6
2
1.4
2
1.1
5
" 100
..
9
6.5
3
1.5
6
"
75
..
2
1 4
9
4.6
8
"
67
..
8
5 7
2
1.1
6
"
69
..
..
..
. .
. .
. .
"
39
..
. .
.. ..
. .
. .
"
9
. .
..
. .
. .
. .
" 105
. .
. .
1
. 5
. .
" 110
.. ..
..
1
.5
2
"
10
..
. .
. .
1
.5
139 100.0 192 100.0 172
GROUP B.
TABLE XIII (b ).
Surface waters impounded in Lake or Reservoir.
—
Quarter
ending 30th
September
1908.
Quarter
ending 31st
December
1908.
Quarter
ending 31s
March
1909.
—
Quarter
ending 30th
September
1908.
Quarter
ending 31st
December
1908
Quarter
ending 31st
March
1909.
N a t u r a l o r u n f i l t e r e dwater
—
B Cloacæ
..
No. of times
isolated.%
No. of times
isolated. %
No. of times
isolated. %
B. Grunthal
60 43.8 21 13.1
10
7 . 7
No. of times
isolated. %
No. of times
isolated. %
No. of times
isolated. % B. Lactis ærogenes
.. 37 27.0 14 8.75 16 12.3
Group I .. 38 27.4 26 16.8 35 27.3 B. Coscoroba ..
..
5
3.7 43 26 8
16 12.3
B. Coli communis
..
8
5.8 24 15 0
17 13.1
"
II ..
5
3.6
6
3.7 ..
.. B. Neapolitanus
..
5
3.7
5 3 1
..
..
B. Pneumoniæ
..
2
1.5
3 1.75 17 13.1
"
III ..
7
5.0 33 20.6 50 39.1 B. Acidi Lactici
..
1
0.7 12 7.5
1
.8
B. Oxytocus Perniciosus,
. .
. . 10 6.25
9
7
"
IV .. 89 64.0 95 59.4 43 33.6
unnamed bacilli.
. .
. .
. .
. .
. .
. .
No.
6
..
" 100
..
1
0.7
2 1.25 ..
..
" 75
.. 14 10.2
7 4.5
2
1.5
"
67
..
3
2.2
8 5.0
21 16.2
"
33
..
10 6.25 11
8.5
"
9
1
0.7
1 0.6
..
..
..
..
..
.. 10
7.7
137 100.0 160 100.0 130 100.0
F i l t e r e d w a t e r —
B. Cloacæ
..
.. 35 83.4
3
9
1
3.1
B. Grunthal ..
..
2
4.7
5
1.5
1
3.1
Group I ..
4
9.1
5
16 10
50 B. Lactis ærogenes
..
1
2.4
9
28
9 28.1
B. Coscoroba ..
1
2.4
1
3 ..
. .
"
II .. ..
..
4
12
. .
.. B Coli, communis
.. ..
..
4
12 ..
..
B. Neapolitanus
..
2
4.7 ..
..
1
3.1
" III ..
2
4.5
3
9
5
25 B. Pneumoniæ
..
. .
..
. .
..
1
3.1
B. Acidi Lactici
..
. .
. .
. .
. .
. .
..
" IV .. 38 86.4 20
63
5
25 B. Oxytocus Perniciosus,
unnamed bacilli.
..
.. ..
.. ..
..
No.
6
• •
1
2.4 ..
.. ..
..
" 100
.. ..
..
7
22
7 21.9
" 75• •
. .
. .
2
6 ..
..
"
"
67
..
..
..
. . ..
2 6.25
"
70
.
. .
..
1
3 ..
..
"
9
.. ..
.. ..
.. 10 31.25
42 100.0 32 100.0 32 100.0
Water that has been through pipes—
B. Cloacæ
..
21 14.2
8
6.7
4
2.8
B. Grunthal ..
.. 62 41.9 25 20.8 25 17.7
Group I .. 67 45.6 25 20.8 31 22.1 B. Lactis ærogenes
.. 13
8.8 11
9.1 15 10.6
B. Coscoroba ..
.. 23 15.5 31 25 8 22 15.6
"
II ..
9
6.1 30 25
13
9 3 B. Coli communis
..
8
5.4 29 23.1 11
7.8
B. Neapolitanus
.
2
1.3 10
8.3 33 23.4
" III .. 14
9.5 13 .10 8 43 30.7 B. Pneumoniæ
..
. .
. .
. .
. .
. .
. .
B. Acidi Lactici
..
4
2.7
. .
..
6
4.3
"
VI .. 57 38.8 53 44.4 53 37.9 B. Oxytocus Perniciosus,
unnamed bacilli.
. .
. .
. .
. .
. .
. .
No.
6
..
1
0 7 ..
..
..
..
" 100
..
1
0.7
1
0 8 12
8.5
"
75
.. 11
7.4
3
2.5
6
4.3
"
67
..
1
0.7
2
1.7
5
3.5
"
38
..
1
0 7 ..
..
5
3.5
"
37
. .
. .
1
. 8
. .
. .
" 33
. .
. .
. .
. .
2
1.4
148 100.0 121 100.0 141 100.0
96
GROUP C.
TABLE XIII ( c).
Well waters or Infiltration gallery water not in a river-bed.
—
Quarter
ending 30th
September
1908.
Quarter
ending 31st
December
1908.
Quarter
ending 31st
March
1909.
—
Quarter
ending 30th
September
1908.
Quarter
ending 31st
December
1908.
Quarter
ending 3
1st
March
1909.
1 . N a t u r a l w a t e r s —
No. of times
isolated. %
No. of times
isolated. %
No. of t
imes
isolated. %
B. Cloacæ
..
16 40.0
4 10
8 1 6
No. of times
isolated. %
No. of times
isolated %
No. of times
isolated. % B. Grunthal ..
..
4 10.0 13 32.5
8
1 6
Group I ..
5 12.5 13 32 5
9
18 B. Lactis ærogenes
..
1
2.5 15 37.5 10 20
B. Coscoroba
..
..
2
5.0
1
2.5
6 12
" II ..
1
2.5
1
2.5
3
6 B. Coli communis.
..
1
2.5
1
2.5
3 6
B. Neapolitanus
.
1
2.5 ..
..
1 2
" III .. 15 37.5
6
15
13
26 B. Pneumoniæ
..
1
2.5 ..
. . ..
. .
B. Acidi Lactici
. .
. .
. .
. .
. . . .
"
IV .
19 47.5 20
50
25
50 B. Oxytocus Perniciosus,
unnamed bacilli.
..
.. ..
.. ..
. .
No.
6
..
1
2.5 ..
..
1 2
" 100
. .
. .
. .
. .
1 2
"
75
..
1
2.5
6 15.0
8 1 6
"
67
. 12 30.0 ..
. .
4
8
40 100.0 40 100.0 50
2. Water that has been through pipes—
B Cloacæ
..
. 17 27.4
2
4.6 14
< t d a l i g n = " r i g h t " > [ i l l e g i b l e ] < / t d >
Group
I ..
3
9.4 13 30.2
8 13.3 B. Grunthal ..
.
11 17.7 13 30.2
7 < t d a l i g n = " r i g h t " > [ i l l e g i b l e ] < / t d >
B. Lactis ærogenes
4
6.5
7 16.3 11 18
"
II .. ..
..
..
..
3
5
B. Coscoroba .
1
1.6
9 20.9
7 11
B. Coli communis
..
7 11.3
. .
..
3
"
III .. 12 37.5 12 27.9 18 13.3 B. Neapolitanus
1
1.6
5 11.6
3
B. Pneumoniæ
2
3.2
5 11.6 ..
"
IV
17 53.1 18 41.9 41 68.3 B. Acidi Lactici
. .
. .
. .
. .
1
B. Oxytocus Perniciosus,
unnamed bacilli.
..
..
. .
. .
1
No.
6
3 49
. .
..
. .
" 100
. .
. .
. .
. .
4
"
75
. ..
. . ..
. .
5
"
67
16 25.8
2
4.6
. .
"
97
..
. .
. .
. . 3
62 100.0 43 100.0 59
101
as the permissible limit for free and. albuminoid ammonia are somewhat too strict for
surface waters in this country. They are, however, applicable to sub-soil waters.
The spring and well waters yield, as a rule, a satisfactory chemical analysis, judged
by the usual standards, but bacteriological analyses show that they are, for the most
part, decidedly inferior to the surface waters. Hence the danger in relying on
chemical analysis only for a water sample in India. A badly contaminated well water
might pass muster, when a thoroughly well-sunned lake water, might be condemned on
its ammonia figures.
26