NationalLibraryOfScotland/medical-history-of-british-india huggingface dataset:
108
extremely hard. It will also be observed that the infant mortality from dysentery
and diarrhœa in both Coonoor and Ootacamund, which are both hill stations, is
considerably above the normal. This is probably due to lack of proper attention
and proper clothing.
The quarter of the year in which the least amount of dysentery and diarrhœa
occurred is, as was the case with cholera, December, January and February ; the,
greatest being registered in September, October and November. It is rather
significant that the same time of the year that is bad for cholera appears also to
be bad for dysentery and diarrhœ a.
The relation between the dysentery and diarrhœa, and cholera figures.----Onew o u l d
naturally expect when a water-supply is known to be contaminated. or to be subject
to outside pollution, that both the cholera and the dysentery and diarrhœa figures
would increase simultaneously. Of this there are several examples in the table
appended. The epidemic cholera that broke out in Trichinopoly in September,
October and November was accompanied by a distinct rise in the death-rate from
dysentery and diarrhœa, but, although cholera persisted during the next quarter, the
death-rate from dysentery and diarrhœa fell off to some extent. The epidemic in
Adòni which took place in August was also accompanied by a very decided rise in the
dysentery and diarrhœa figures for that month. We have already seen that this
outbreak was probably due to the town pipe supply. There is no particular
parallelism between the figures for cholera and the figures for dysentery and diarrhœa
in the town of Madras. The reason is that the water in Madras is
always so extremely bad that fluctuation of the dysentery and diarrhœa figures
does not occur to any great extent. The total figures for these diseases, it will be
observed, are high. The epidemic that occurred in Guntùr in June, July and
August was marked by a similar rise in the dysentery and diarrhœa figures. It will
be observed that in nearly all the cases mentioned above, where the increase in
the deaths from dysentery and diarrhœa has taken place simultaneously with the
epidemic of cholera, that the death-rate of children under five has increased to a very
great extent. It would therefore seem that, the presence of cholera, the increase in
the number of deaths from diarrhœa and dysentery, and the very marked increase in the
number of deaths from these latter diseases in children under five, point to the fact that
the water-sapply is at fault, and is probably spreading the infection. This rule does
not hold good absolutely, for the town of Madura furnishes an exception. But
whenever these three facts are noticed simultaneously very careful examination of
the water-supply should be undertaken.
110
List of Media used in the work described in this report.
I. (Stock solution A).—Bile salt glucose peptone neutral red broth—
Peptone
...
...
...
...
...
... 60 grms.
Sodium taurocholate ...
...
...
... 15 ,,
Glucose
...
...
...
...
...
. . . 15 ,,
Water
...
...
...
...
...
...
1 litre.
Neutral red
...
. . .
...
...
... 10 cc. of 5 per cent. solution.
Normal sodium
...
...
...
...
... Till neutralised as indicated
by the neutral red.
Carbonate solution ...
. . .
...
...
...
(B Solution) —
2 parts of A solution + 1 part of water.
(C Solution)—
1 part of A solution + 1 part of water.
(D Solution) —
1 part of A solution + 2 parts of water.
II. Bile salt lactose peptone neutral red broth—
Same as above lactose being substituted for glucose.
III. Bile salt lactose peptone agar—
Peptone
...
...
...
...
. . .
... 20 grms.
Sodium taurocholate
...
...
. . .
..
5 ,,
Lactose
...
. . .
...
...
... 10 ,,
Agar
...
. . .
...
. . .
... 20 ,,
Water
...
...
...
. . .
. . .
...
1 litre.
Neutral red ...
...
...
...
...
... 10 cc. of 5 per cent. solution.
Normal sodium
...
...
...
... ... Till neutral point is reached
as indicated by the neutral
red.
Corbonate solution ...
...
. . .
. . . ...
IV. Nutrient agar—
Agar
... ... ... ...
...
20 grms.
Lemco
...
. . .
. . .
. . .
. . .
5 ,,
Peptone
...
...
...
. . .
. . .
10 ,,
Sodium chloride ...
...
...
. . .
5 ,,
Water
... ... ... ...
1 litre._
Standardised to + 10 using normal sodium corbonate solution.
V. Nutrient broth—
Peptone
...
...
...
...
...
... 10 grms.
Sodium chloride
. . .
...
...
...
. . . .
5 ,,
Lemco
...
...
. . .
...
...
. . .
5 ,,
Water
...
. . . ...
. . .
...
1 litre.
Standardised to + 10 using normal solution of sodium carbonate.
VI. Bile salt peptone neutral red sugar medium—
Peptone
...
. . .
...
...
. . .
... 10 grms.
Bile salt
...
...
. . .
...
. . .
...
5 ,,
Sugar
...
...
. . . ...
...
...
5 ,,
Water
...
...
. . . ...
. . .
...
1 litre
Neutral red ..
... ...
...
...
...
5 cc. of 5 per cent. solution.
To prepare different sugars, the particular sugar is substituted in the column of
sugar in the above solution.
The following sugar media are made
—
1.Saccharose.
2.Dulcite.
3.
3. Adonite.
4.Inulin.
VII. Glucose medium for Voges and Prosk. reaction—
Peptone
...
...
...
...
...
... 10 grms.
Glucose
...
...
...
... ...
... 5 ,,
Water
. . .
...
. . .
... ...
...
1 litre.
VIII. Strong peptone solution for vibrios—
P e p t o n e . . .
...
...
. . .
. . .
... 100 grms.
Sodium chlorid
...
...
...
...
... 50
,,
Water
...
...
...
...
...
... 850 ,,
10 cc. of this in 90 cc. of suspected water converts latter into 1 per cent. peptone water.
PART II.
SEPARATE ANALYSES OF THE VARIOUS
WATER SUPPLIES.
GROUP A.
RIVER WATERS.
113 ti
N amber Coe. I.
08.
COCA NADA.
Sample taken-16th August 1908.
Remarks.—Water is taken from the Samalkot Irrigation canal that comes from the Gódávari.dávari.
Both river and canal are now in high flood, there being much rain in the source
of the river. The water is run into a tank for storage and settlement. It is then
passed through filter beds and is supplied to the town. The supply is intermittent.
Recent rainfall in the district, • 75 of an inch on 18th August.
A. Sample I.—Storage Tank unfiltered water.
Colony
No.
Saccherose.Dulcit. Adonit. Inulin.
Voges and
Prosk.
Mobility.
Group.
Bacillus.
G r o u p a n d
R e m a r k s .
(a) Total colonies on agar at 37°—280 in 1 cc.
( b ) Bile salt glucose, medium.
1 - - - - - -
I B. Grunthal.*
Group I
+
-
2 + --- -
+
- IV B. Cloacæ. *
" II
3 - - - - + -
I
" III
1 tube 20 cc. of water taken ..
1 ..
4 - - - - - -
I B Grunthal
.*
" IV
2 tubes 10 cc. "
2 ..
5 + - - -
+ +
IV B. Cloacæ.
4 „
5 cc. "
..
4 ..
6 + - - - + - IV
Do.
*
5
„
1 cc. "
3
2
5
„ .1 cc."
1
4
5 „ .01 cc. ,, ..
..
5
(c) Fœcal bacilli in .1 cc. and upwards.
(d) Sporogenes milk test +
in 20 cc.
B. Sample II.—Collecting Well after filter beds
filtered water.
1 +
- - -
+
-
IV B. Cloacæ. *
Group I
2 + - - - + + IV
Do.
*
" II
( a) Total colonies on agar at 37°—380 in 1 cc.
3 + - - - + - IV Do.
*
" I I I
(b ) Bile salt glucose, medium.
4 + - - - +
- IV
Do.
*
"
IV
+ -
5 +
- - - +
- IV
Do.
6 + - - - + + IV
Do.
1 tube 20 cc. of water taken ..
1
..
7 +
- - -
+ + IV
Do.
2 tubes 10 cc.
.. 2 ..
8 +
- - - + - IV
Do.
*
4
„
5 cc. " ..
4 ..
9 + - - -
+
- IV
Do.
*
5 " 1 cc. " ..
5 ..
10 + - - - + - IV
Do.
*
5 „
.1 cc. " ..
5 .
.
5
" .01 cc "
3
2
(c) Fœcal bacilli in .01 cc and upwards.
( d) Sporogenes milk test + in 20 cc.
C Sample III.—Pillar Fountain near the Cocanad
a Port Railway Station
1 +
- - -
+ +
I V
B. Cloacæ.*
Group I
2 + - - - + + IV
Do.
" II
(a) Total colonies on agar at 37°—120 in 1 cc
3 + - - - + +
I V
Do.
" III
( b ) Bile salt glucose, medium
4 + - - - + +
I V
Do.
" IV
+
-
5 + - - -
+
+
I V
Do.
6 + - - - - -
I V B. Coscoroba.
1 tube 20 cc. of water taken
..
1 ..
7 - - - - - -
I
B. Grunthal.*
2 tubes 10 cc.
"
..
2 ..
8 - - - - - -
I Do.
4
„ 5 cc.
"
. . 4 ..
9 - - - - - -
I Do.
5
„
1 cc.
" ..
5 ..
1 0
- - - - - -
I Do.
5
„ .1 cc. "
5 ..
5
„ .01 cc. " ..
5 ..
(c) Fœcal bacilli in .01 cc. and upwards.
( d) Sporogenes milk test +
in 20 cc.
D Sample IV.—Fountain in front of the Veterinar
y
Hospital, Cocanada Town .
1 + - - - + -
I V
B. Cloacæ.*
Group I
2 + - - - + +
I V
Do.
" II
(a) Total colonies on agar at 37° 210 in 1 cc
3 + - - - + +
I V
Do.
" III
(b) Bile salt glucose, medium.
4 + - - - + +
I V
Do.
" I V
+ -
5 + - - - + -
I V
Do. *
6 + - - - + -
I V
Da. *
1 tube 20 cc. of water taken
..
1 ..
7 + - - - + -
I V
Do.
*
2 tubes 10 cc.
"
..
2 ..
8 + - - - + -
I V
Do.
*
4
" 5 cc. " ..
4
9 + - - - + -
I V
Do. *
5 " 1 cc "
5 ..
1 0
+ - - - + -
I V
Do. *
5
„ .1 cc. "
5 ..
5
" .01 cc. " ..
..
5
(c) Fœcal bacilli in .1 cc. and upwards,
(d) Sporogenes milk test +
in 20 cc.
•
.
.
.
.
.
•
•
.
,
..
.
.
.
REPORT,
115
.
( i ) T h e U n f i l t e r e d w a t e r i n t h e s t o r r a g e t a n k s h o w s p o l l u t i o n s o f a m o d e r a t e r a m o u n t . F æ c a l b a c i l l i a r e
preset in 1 cc.
( i i ) T h e f i l t e r e d w a t e r i s c o n s i d e r a b l y w o r s e t h a n t h e u n f i l t e r e d w a t e r , a s i t h a s f œ c a l b a c i l i l i n 1 / 1 0 0 o f a c c .
.
ii) The filtered water is conside worserably worse than the unfiltered water, as it has faecal bacilli in 1/100 of a cc.
...
filters
hers are actually adding bacilli to the water and these of an undesirable nature.
(iii) The samples taken from the pipe line gave the same results as the filtered water so that the intermittent
a pply, though very undesirable, does not seem to be adding further pollution to the ;water.
Recommendations.—(i) A sample of filter sand be sent to the Sanitary Commissioner for inspection.
.
(ii) It is in all probability too coarse, if that is so, 12" of fine sea sand must be substituted.
(iii) When this is being done, the deep layers should be removed, washed up and replaced.
COCANADA.
Samples taken—18th November 1908.
Remarks.—There has been about 14 inches of rain since last sample was taken but has been less
than 2 inches since October 1st. The water in the canal is now not flood water.
The storage reservoir holds about one week's supply.
The supply is intermittent.
118
BACTERIOLOGICAL ANALYSIS.
S a m p l e I . — S a m a l k o t a C a n a l .
C o l o n y
N o .
S a c c h e r o s e .
D u l c i t .
A d o n i t .
A n u l i n . V o g e s a n d
P r o s k . M o b i l i t y .
G r o u p .
Bacillus.
Group and
Remarks.
(a) Total colonies on agar at 37°—1,890.
1
— — — — — —
I B. Grunthal. *
Group I
( b ) Bile salt lactose, medium.
+
—
2
— — — — — —
I
Do.
"
II
3
— — — — — —
I
Do.
" III
1 tube 20 cc. of water taken
1 ..
4 — —
—
— — —
I
Do.
" IV
2 tubes 10 cc. ,, ..
2 ..
5 — — — — — —
I
Do.
4 „ 5 cc. "..
3
1
6
— — — — — —
I
Do.
5 „ 1 cc. "..
3
2
7
— — — — — —
I
Do.
5 " .1 cc. "..
..
5
8 — — — — — —
I
Do.
5 „ .01 cc. " 74
..
5
9
— — — — — —
I
Do.
10
—
— — — — —
I D o .
(c) Fœcal bacilli in 1 cc. and upwards.
(d ) Sporogenes milk test —in 20 cc.
Sample II.—Storage Tank—Unfiltered Water.
1
— — — — — —
I B. Grunthal.*
Group I
2
— — — — — —
I
Do.
"
II
( a) Total colonies on agar at 37°—170.
3
— — — — — —
I Do.
"
I I I
4
— — — — —
+ II B. Grunthal.
" IV
(b) Bile salt lactose, medium.
+
—
5
— — — — — —
I B. Grunthal.*
6 — — — — —
I
Do.
1 tube 20 cc. of water taken..
1 ..
7
— — — — — —
I
Do.
2 tubes 10 cc. "..
2 ..
8
— — — — — —
I
Do.
4 " 5 cc.
,,.. ..
4
9
— — — — — —
I
Do.
5 „ 1 cc. "..
..
5
1 0
— — — — — —
I
Do.
5 „ .1 cc. ,,
..
5
5 „ .01 cc. ,,
..
5
( c) Fœcal bacilli in 10 cc. and upwards.
(d) Sporogenes milk test — in 20 cc.
Sample III.—Collecting well after Filter Beds—
Filtered Water.
1 +
— —
— + — IV B Cloacæ. *
Group I
2 + — — — + — IV
Do.
„ II
(a) Total colonies on agar at 37°—300.
3 + — — — + — IV
Do.
" III
4 + — —
—
+ — IV
Do.
„ IV
( b) Bile salt lactose, medium.
+
—
5 + — — — +
—
IV
Do.
6 + — — — + — IV
Do.
1 tube 20 cc. of water taken.. 1 ..
7 + - — — +
—
IV
Do.
2 tubes 10 cc. " ..
2 ..
8 + — — — + — IV
Do.
4 " 5 cc.
„ ..
4
..
9 +
—
— — + + IV B . C l o a c æ .
5 „ 1 cc. " .. 3
2
10 + — — — + — IV B . C l o a c æ . *
5 " .1 cc. " ..
2
3
5 „ .01 cc."
. .
5
(c) Fœcal bacilli in .3 cc. and upwards.
( d) Sporogenes milk test — in 20 cc.
Sample IV.—Pillar Fountain near Port Railway
Station.
1 + — — — + — IV B. Cloacæ.*
Group, I
2 + — — — + — IV
Do
"
I I
(a) Total colonies on agar at 37°—110.
3 +
—
—
— + — IV
Do
" III
4 + + — — + — III
" IV
( b) Bile salt lactose, medium.
+ —
5 + + — — + — III
6 + + — — + — III
1 tube 20 cc. of water taken..
1
. .
7 + + — — + + III
2 tubes 10 cc. "..
2
..
8 + + — — + + III
4 „ 5 cc. " ..
4 . .
9 + + — — + + III
5 „ 1 cc. " ..
2
3
10 + — — — + — IV B. Cloacæ. *
5 „ .1 cc. " ..
1
4
5 „ .01 cc. " .. 1
4
(c) Fœcal bacilli in 1 cc. and upwards.
( d) Sporogenes milk test —in 20 cc.
Sample V.—Fountain in front of Veterinary Hos-
pital
Colony No.
S a c c h e r o s e .
D u l c i t .
Adonet.
Inulin.
Voges or
Prosk. M o b i l i t y .
G r o u p .
Bacillus.
.
Group and
Remarks.
(a) Total colonies on agar at 37°—3,150.
(b) Bile salt lactose, medium.
1 + — — — + —
IV.
B . C l o a c æ *
Group I nil.
+ —
2 + — — — + — IV
Do.
" II nil.
3
+ - -
—
+
—
IV
Do.
" III nil.
1 tube 20 cc. of water taken ..
1 ..
4 + — — — + — IV
Do.
„ IV 10.
2 tubes 10 cc.
,,
• •
2 ..
5 + — — — + — IV
Do.
4 " 5 cc. ,, —
4 ..
6 + — — — + — IV
Do.
5 ,,
1 cc.
,,
.
3
2
7 + — — — + — IV
Do.
5 „
•1 ce.
,,
• • ..
5
8 + — — _ + _
I V
Do.
5
•01. cc.
• • ..
5
9 + — — —
+ — IV
Do
10 + — — — +
—
IV
Do.
(c) Fœcal bacilli in 1 cc. and upwards.
(d) Sporogenes milk test — in 20 cc.
REPORT.
(i) The canal water is fairly good ; it contains fœcal bacilli in 1 cc.; it has all the characteristics of a well-sunned and
water. Total bacilli are under 2,000 per cc.
(ii) The water in the storage tank is much better than the unstored tank water ; it contains fœcal bacilli in 10 cc. and
tal bacilli have fallen to 170 per cc. The improvement is due to storage and settlement.
(iii) The filtered water is very much worse than the storage tank samples ; there are fœcal bacilli in .3 cc. This
een found on inspection to be due to the fact that the filter beds and filtered water tank are not tight and subsoil water
its way through the cracks in the masonry and pollutes the water. Fortunately the subsoil water is not a very bad one
originally comes from the large storage tank. But the presence of these cracks is a very serious defect and may at
time lead to pollution of a dangerous nature.
(iv) The samples from the distributary system are much the same as the filtered water.
Recommendations—
(i) The completion of the two new filter beds be expedited as much as possible and that they be laid down with
rials as suggested in my note.
(ii) When the new beds are ready the filtering material from the old beds should be removed and the Sanitary
neer's opinion should be asked as to what is required to make them water tight
(iii) The inflow of subsoil water to the filtered water tank be prevented.
120
CHEMICAL ANALYSIS,
Sample No. 2.
Cocanada
Physical
appearances.
Colour and
Transparency.
White and hazy.
-
Smell.
None.
,
Quantitative.
Total solids
. . .. Parts per
Temporary hardness ..
. . Do.
Permanent hardness ..
. . . Do.
Total hardness
. . . . Do.
Chlorine
. . .. Do.
Free ammonia
..
. . Do.
Albuminoid ammonia..
Oxygen absorbed (Tidy's Process)
Do.
Nitrates..
.
100,000.
do.
do.
do.'
do.
do.
do.
do.
do.
13.600
4.000
1.000
5.000
0.710
0.003
0.031
0.066
Nil.
Qualitative.
.
Nitrites..
. . .
Sulphates
. . . . . . . .
Phosphates
. . . .
.
. . .
Iron, poisonous metals
..
. . . .
.
.
Nil.
Present.
Nil.
Nil.
Sample No. 4.
Cocan
"
Physical
appearances.
Colour and
Transparency.
Slightly yellowish but clear.
Smell.
None.
Quantitative.
Total solids
..
.. Parts per
Temporary hardness ..Do.
. .
Permanent hardness..
Total hardness
. .Do. . .
Chlorine ..
. . . . Do.
Free ammonia
. . 1)o. . .
Albuminoid ammonia
. . Do.
Oxygen absorbed (Tidy's Process)
Do.
Nitrates
..
. . Do.
100,000.
do.
do.
do.
do.
do.
do.
do.
do.
1 1 . 0 0 0
Qualitative.
Nitrites
. . . . .. ..
Sulphates
. -
. . .. ..
Phosphates
..
. . . . . .
Iron, poisonous metals
... .
. .
Nil.
Present.
Nil.
Nil.
Coca. I
No.
09.
COCAN ADA.
Samples taken—10th February 1909,
Remarks :. —No rain has fallen during the last three months ; the canal is now very low.
BACTERIOLOGICAL ANALYSIS.
I.—Samalkota Canal.
Sample
(a) Total colonies on agar at 37° —520.
(b) Bile salt lactose, medium.
+
—
1 tube 20 cc. of water taken
,, 1 . .
2 tubes 10 cc.
,,
..
2 ..
4 "
5 cc.
4 ..
,,
5„
1 cc.
,, .
,
5 ..
5 "
.1 cc...2
8
'
5
.01 cc.
,,
..
1
5
(c) Fœcal bacilli in 1 cc. and upwards.
(d) Sporogenes milk test + in 20 cc.
C o l o n y
No .
S a c c h a r o s e .
D u l c i t .
A d o n i t e .
I n u l i n .
V o g e s
a n d
p r o s k .
I n d o l .
M o b i l i t y .
G r o u p .
Bacillus.
Groups
Remarks.
1 + — — —+ + — IV
2 + – – – – – + IV
3 + — — — + + + IV
4 + — + — + + — IV
5 – – – – – + +I
6– – – – – – – I
7– – – – – +— I
8 – – – – + – I
9 – – – – – + —I
10 – – – – – + — I
B. Cloacæ.*
B. Coscoroba.
B Cloacæ.
B. Laotis ærogenes or
capasulatas.
B. Grunthal
Do.*
Do*
Do.*
Do.*
Do.*
Group I,
,,
II),
„ III,
, , I V ,
I V,
Sample II.—Storage Tank—Unfiltered water.
(a) Total colonies on agar at 37°— 4,310.
(b) Bile salt lactose, medium.
_
1 tube 20 cc. of water taken
..
1 ..
2 tubes 10 cc.
,,
. . ..
4 "
5 cc.
,,
..
4 . .
5 „
i
cc.
,,
. . 5 . .
5 „
.1 cc.
,,
.. ..
5
5
.01 cc.
,,
. . . . 5
(c) Fœcal bacilli in 1 cc. and upwards.
(d) Sporogenes milk test—in 20 cc.
1 + — — — — + + IV
2 + — — — — + + IV
3 — — — — — + +
I
4 — — — — — + +
I
5 + — + — — + + IV
6 + — — — — + + IV
7 + — — — — + + IV
8 + — + — + + + IV
9 + — — — + — + IV
10 + — + — + + + IV
B. Coscoroba.
Do.
B. Grunthal.
Do.
B. Coscoroba.
Do.
B. Laotis ærogenes or
capsulatus.
B. Cloacæ.
B. Lactis ærogenes or
capsulatus.
Group I,'
,, II,'
„III,,
„ IV
Sample III.—Subsoil water from the excavation
adjoining the new filter beds.
(a) Total colonies on agar at 37°-5,040.
(b) Bile salt lactose, medium.
+-
1 tube 20 cc. of water taken
..
1 ..
2 tubes 10 cc.
,,
..
2 ..
4 „
5 cc.
,,
.
4 ..
5 „
1 cc.
,,
..
5 . .
5
. ,, . 1 cc.
,,
. . 5 . .
5 „
.01 cc.
,, .
.
5
..
(c) Fœcal bacilli in 01 cc. and upwards,
(d) Sporogenes milk test + in 20 cc.
1 — — + — — + +
I
2 — + — — — + — II
3 – – – – – + –
I
4 – – – – – + — I
5 – – – – – + + I
6– – – – – + -
I
7 – – – – – + —
I
8 — — + — — + +
I
9 + – – – + + + IV
10 – – – – – + —
I
B. Acidi lactici. *
B. Colicommuni etc.
B. Grunthal *
Do.
*
Do.
Do.
*
Do.
*
B. Acidi lactici*
B. Cloacæ.
B. Grunthal.*
Group I
„ .II
„ III
„ IV
Sample IV.—Collecting well after Filter beds—
Filtered water.
(a) Total colonies on agar at 37°—150.
(b) Bile salt lactose, medium.
(b)
+ –
1 tube 20 cc. of water taken .. 1 ..
2 tubes 10 cc...
2 . .
, ,
4 „
5
4 ..
.
.
5 „
1 cc.
„
..
5 ..
5 „
.1 cc.
..
5 ..
, ,
5 „ .01 cc.. .
5 ..
,,
(c) Fœcal bacilli in .01 cc. and upwards.
(d) Sporogenes milk test + in 20 cc.
1 + + + — + + —III
2 + + + — + + — III
3 + + + — ++ — III
4 + + + — + + — III
5 + + + — + + — III
6 + + + — + + — III
7+ + + — + + — III
8 + + + — + +– III
9 + + + — + + — III
10 + — + — + + + IV B. Lactis æ rogenes,
or capsulatus.
Group
I
I I I
I I I
, , I V .
1 2 2
123
Sample v.
—Pillar . Fountain near Port Railway
-
Station.
C o l o n y n o .
Sa c c ha r os e . D u l c i t . A d o n i t .
I nul i n.
V o g e s a n d P r o s k .
I n d o l .
Mot i l i t y.
G r o u p .
Bacillus. G r o u p
and
Remarks.
Total colonies on agar at 37°—160.
, Bile salt lactose, medium.
1 2
3
4
5
6
7
8
9
10
—
—
—
—
-
—
—
—
—
—
-
—
—
+
+
+
+
+
—
—
—
—
-
—
—
—
—
—
—
-
—
—
+
+
+
+
+
+
+
+
+
+—
—
—
—
-
—
_
—
—
—
I
I
I
I
I
I
I
I
I
I
B. Grunthal.*
Do.*
B. Acidi lactici.
Do.
Do.
Do.
Do.
Do.
B. Grunthal.*
D
*
o.
Group I, 10.
„ II, nil.
„ III, nil.
„ IV, nil.
—
I tube 20 cc. of water taken
..
1 ..
2 tubes 10 cc.
,,
4„
5 CC.
,,
. .
4 ..
5 ,,
1 cc.
,,
. . ..
5
5,,
.1 cc
,,
5 „ .01 cc.
,,
Fœcal bacilli in 5 cc. and upwards.
Sporogenes milk test —
in 20 c.o.
Sample
Sample VI.—Fountain in front of Veterinary
Hospital.
1 + — — — — + — IV
2 + — -- — + — . IV
3 + — — — — + — IV
4 + — — — + - IV
5 + — – — + — IV
6 + — — —+ + — I v
7 + — — — + +- — IV
8 + —
— + + — IV
9 + —
- — + - IV
10 + — — — — + — IV
B. Coscoroba.
Do.
Do.
Do.
Do.
B. Clcacæ
*
Do .
Do.
B, Coscoroba.
Do,
Group I. nil.
I
„ II, nil.
„ III, nil.
„ IV, 10.
Total colonies on agar at 37°-190.
(b
Bile salt lactose, medium.
1 tube, 20 cc. of water taken ..
1 1 . ..
2 tubes 10 cc.
,,
2 . .
4 „
5 cc.
,, . .
4 .
5 „
1 cc.
,,
..
5 ..
5 „
.1 cc.. . ..
5
5 „ .01 cc.
I,
.
..
5( c )
Fœcal bacilli in 1 cc. and upwards.
Sporogenes milk test — in 20 cc.
REPORT.
T h e
sample from the Samalkota canal shows a considerable amount of pollution containing fœcal bacilli in 1 cc. The
anisms present however suggest that the water has been well sunned.
The samsple from the storage tank is about the same in quality, fœcal bacilli being present in 1 cc. This is worse
the same analysis last quarter, the tank being newly replenished.
. The sample taken after the filtration shows as usual that the water has been spoilt by the process ; fœcal bacilli are
present in .01 cc.
4.
4. The two samples of water taken from the pipe line show a considerable improvement on the filtered water, fœcal
bacilli
being present in 5 cc. in one case and in 1 cc. in the other. This is due to the action of the pipes on the water.
Thee sample taken of the subsoil water from the excavation near the new filter bed shows that the water is subject to
niderable pollution and that dangerous organisms are met with in the ground supply.
Chemically the water cannot be called good.
124
CHEMICAL ANALYSIS.
Sample No. II.
Cocanada
Physical
appearances.
Colour and
Transparency.
Yellowish and basy.
Smell.
None.
Quantitative.
Total solids. .
..
. '
Temporary hardness
. .
. .
Permanent hardness
. .
. .
Total hardness
. . :
Chlorine
. .
. .
Free ammonia
. .
. .
Albuminoid ammonia
..
..
Oxygen absorbed (Tidy's Process) ..
Nitrates (as N 2 0 5 )
. -
. .
Parts per 100,000.
Do.
do.
Do.
do.
Do.
do.
Do.
do.
Do.
do.
Do.
do.
Do.
do.
Do.
do.
16 . 600
4.750
0.750
5 500
1.065
0.004
0.023
0.135
Trace.
Qualitatative.
Nitrites
. . . . . . .. . .
Sulphates
. .
. .
. ,
. .
Phosphates
. . . . . . . . . .
Iron, poisonous metals
..
. . . . . .
Nil.
Present.
Nil.
Nil.
Sample No, V,
Cocanada
Physical
appearances.
Colour and
'Transparency.
Yellowish but clear.
Smell.
None.
Quantitative.
Total solids
..
. .
..
Temporary hardness
..
Permanent hardness
..
. .
Total hardness
..
. .
Chlorine
. .
..
Free ammonia
. .
..
Albuminoid ammonia
..
. .
Oxygen absorbed (Tidy' 3 Process) ..
Nitrates (as N 2 0 5 )
. .
. 0
Parts per 100,000.13.400
Do.
do.
Do.
do.
Do.
do.
Do.
do.
' Do.
do
Do.
do.
Do.
do.
Do.
- do.
4.750
0.750
5 500
1.065
0.004
0.020
0.064
0.096
Qualitative.
Nitrites
..
..
. .
. .
. .
Sulphates
..
..
..
..
Phosphates
. .
. .
. .
. . . .
Iron, poisonous metals
..
. .
. . . .
Nil.
Present.
Nil.
Nil.
Number
Con . I.
08.
CONJEEVERAM.
Samples taken-4th August 1908.
Remarks —Water is taken, from the Vaguvattru across which an infiltration gallery is thrown.