61
Experiments No. 32.
A single sample of human faces with a layer of sand. placed at the bottom of
the dish.
Emulsion before exposure contained.
3 lactis æ rgenes, 2 grunthal, 1 No. 67, 4
acidi lactici.
After 1 hour's exposure showed
... 1 coli communis, 2 acidi lactici. 1 No. 67, 6
grunthal.
„ 2 hours'
1 lactis æwrogenes, 1 acidi lactici, 1 cloacw, 1
coscoroba. 6 grunthal.
Noon of thc second day (after 8 hours' 1 lactis ærogenes , 1 coli communis, 2 acidi
• exposure) showed.
lactici, 6 grunthal.
Evening of the second day showed ... 1 acidi lactici, 1 neapolitanus, 3 coscoroba,.
5 grunt hal.
Noon of the third day showed
... 7 coscoroba, 1 cloaca, 2 grunthal.
Evening of the third day showed ... 3 acidi lactici, 5 coscoroba, 2 grunthal.
fourth
5)
3 acidi lactici, 3 coscoroba, 3 grunthal, 1
, ,
fifth
,,
3 grunthal, 5 coscoroba, 1 acidi lactici, 1
pneu-moniæ.
sixth
... 6 grunthal, 1 cloacæ,3 coscoroba.
,,
seventh
... 7 grunthal, 2 coscoroba, 1 neapolitanus.
Subsequent samples proved sterile in 20 cc.
Note.—Disappearance of organisms less rapid than usual due to the presence of sand ; much
later disappearance of coli communis, namely at 8 hours ; no subsequent appearance after this. This
delayed disappearance is due to the presence of sand in the bottom of the dish ; the usual appearance
and disappearance of cloaca, lactis ærogenes and. particularly coscoroba, which at one time decidedly
predominated ; increase in number of grunthal for the first 4 or 5a n a l y s e s , t h e n s l i g h t
dereasefollowed by final survival of these organisms ; coscoroba and neapolitanus in small numbers also
appeared towards the end..
Experiment No. 33.
Mixture of 5 samples of human faces with a layer of sand. at the bottom :—
Emulsion before exposure contained.
1 coli communis, 3 acidi lactici, 1 cloaca,
5 grunthal.
After 1 1/2 hours' exposure showed ... 1 coli communis, 5 grunthal, 3 acidi lactici,.
1 cloak;.
„ 22 „
17
... 1 acidi lactici, 2 No. 67, 7 grunthal.
,,
2
... 2 midi la tici, 1 cloaca, 7 grunthal.
Noon of the third day
... 2 coli communis, 3 acidi lactici, 1 coscoroba,
4 grnnthal.
Evening of the third day showed ... 9 grunthal, 1 cloacw.
,, fourth . .• 8 grunthal, 2 cloacm.
fifth
,, ••• 5 acidi lactici, 5 grunthal.
sixth
3 acidi lactici, 7 grunthal.
,,
seventh
„
... Sterile.
Note.—Very slight variation in number f grunthal from beginning to end of the experiment,.
the persistence of coli till the end of the second day. but not longer (as in then experiments ; com-
paratively late persistence of acidi lactici; usual appearance and disappearance of No 67 and cloacae.
Note the alteration in numerical relation of bacilli to one another in the first
hours is nil.
Experiment No. 34.
A single sample of human fceces with a layer of sterile sand at the bottom of
the dish.
Emulsion before exposure contained.
3 acidilactici, 2 coscoroba, 4 grunthal, 1 No. 6.
Alter 1. hour's exposure showed
... 1 lactis ærogenes, 2 coscoroba, 1 No. 75, 6
grunthal. ,,
2 hours'
,, ...
3 lactis ærogenes, 8 grunthal, 2 No. 75.
3
,, ,,
2 lactis ærogenes, '2 cloac a, 1 No. 76, 2
coscoroba, 3 grunthal
... 7 g,runtual, coscoroba, No 67, 1 No. 6. ,,
77
77
Noon of the second day (after 11 7 grunthal, 2 coscoroba, 1 lactis ærogenes.
hours' exposure).
Evening of the second day ...
... 6 grunthal, 3 acidi lactici, 1 No. 101.
Noon of the third day
...5 acidi lactici, 5 grunthal.
Evening of the fourth day
4 acidi lactici, 1 cloacw, 5 grunthal.
16