EXPLANATION OF PLATE I .
Figure 1.—Poiymorphonuclear leucocyte containing six parasites. Stained by Romanowsky's stain.
Magnification 550 diameters.
Figure 2.—Endothelial cell containing forty-nine parasites. Stained by Romanowsky's stain.
Magnification 550 diameters.
Figure 3.—Eosinophil leucocyte containing two parasites. Stained by :Romanowsky's stain.
Magnification 550 diameters.
Figure 4.—Endothelial cell containing thirty-two parasites, six of which are lying on the nucleus.
Stained by Romanowsky's stain. Magnification 550 diameters.
Figure 5.—Large blue body containing two parasites from the peripheral blood of patient mentioned
in text. Stained by Romanowsky's stain. Magnification 550 diameters.
.Figure 6.—Large mononuclear leucocyte much distorted. containing ten parasites ; note two of the
parasites show commencing development. Stained by Romanowsky's stain. Magnifica-
tion 550 diameters.
:Figure 7.—Parasites showing the early stages of development. Stained by Giemsa's stain.
Magnification 1,000 diameters.
(a) Two unchanged parasites lying in a disintegrating leucocyte 80 hours after being
ingested.
(b) Parasite with a large central vacuole lying in a degenerating leucocyte ; the
macronucleus shows commencing enlargement.
(e) Five parasites lying grouped together in the remains of a leucocyte ; four of the
cells are much enlarged, their protoplasm is vacuolated, the flagellar vacuoles are
well developed, and the micronuclei show evidences of commencing division;
the fifth parasite is almost unchanged.
(d) Enlarged parasite lying in a leucocyte, the macronucleus is granular and situated
across the cell ; the protoplasm stains dark blue and contains a number of large
circular vacuoles.
(e) A similar parasite.
(f) Much enlarged parasite with deep blue granular protoplasm and large circular
vacuoles ; the flagellum is commencing to form.
Figure 8.—Parasites showing the early formation of the flagellum. Stained by Giemsa's stain.
Magnification 1,000 diameters.
(a) Enlarged parasite with an elongated macronucleus surrounded by groups of
vacuoles. Adjacent to the micronucleus is the circular flagellar vacuole which
consists of dark strands lying in a light pink matrix.
(b) Enlarged oval parasite with two micronuclei close to which is the developing
flagellum.
(e) Parasite showing the very earliest appearance of the flagellum which is seen as
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a light pink area with a darker centre lying in the blue staining protoplasm.
(d) Parasite very similar to (a), the micronucleus is thickening prior to division.
(e) Two parasites showing the early development of the flagellum; in one there are
two micronuclei.
(f) A similar parasite, the macronucleus shows evidences of division.
-Figure 9.—Parasites showing the early protrusion and growth of the flagellum. Stained by Giemsa's
stain. Magnification 1,000 diameters.
(a) Parasite showing the flagellum protruding as a short thick brush of fine filaments.
(b) Twin parasites showing the early protrusion of the flagellum ; in one it can be
traced into the vacuole to a point close to the micronucleus.
(c) Parasite showing the flagellum passing into the vacuole to a point in close
proximity to the micronucleus.
(d) Large vacuolated parasite showing the flagellar vacuole passing to the margin
prior to rupture.
(e) Circular parasite with flagellum seen as a short curved rod ; the macronucleus is
dumb-bell shaped and is about to divide.
(f) Large' parasite with short flage llum which can be traced up to a chromatic dot
close to the micronucleus ; the macronucleus has just divided.
Figure 10.—Parasites showing the method of formation of the true rosette. Stained by Giemsa's
stain. Magnification ,000 diameters.
(a) Large oval cell consisting of deep blue granular protoplasm containing a number
of small circular vacuoles. The original macronucleus has divided and one of
the daughter nuclei is about to divide; associated with the three micronuclei there
are three large flagellar vacuoles.
(b) Large parasite with two macro-and micronuclei, the former show signs of
commencing division ; there are no flagellar vacuoles.
( c) A similar cell ; note that the macronuclei are situated at opposite poles.
(d) Large vacuolated parasite showing the recent division and commencing separation
of the micronuclei.
(e) Elongated parasite, one of the daughter nuclei is commencing to divide. The two
micronuclei have already divided.
( f) Oval cell showing division of a daughter nucleus ; the micronucleus is thickened
and elongated prior to transverse division.