59
In the following experiments a single specimens of cowdung was taken, but a
layer of sterile sand was placed at the bottom of the dish :—
Experiment No. 25.
Emulsion before exposure contained.
5 lactis æærogenes, 2 No. 100, 3 No. 67.
After 1 hour's exposure showed
... 5 lactis ærogenes, 3 No. 67, 2 No.100.
„ 2 hours'
„
... 7 lactis æærogenes. 2 No. 100, 1 grunthal .
. . . 10 lactis æærogenes.
,,
,, ,,
,,
,,
,,
... 3 lactis æwrogenes, 3 No. 67, 2 cloace I grunthal.
Noon of the second day (after 10 3 lactis æærogenes, 4 cloacae, 1 neapolitanus,
hours' exposure) showed.
2 coscoroba.
Evening of second day showed
... 5 lactis ææ, 3 cloacae, 1 coscoroba, 1
neapolitanus.
Evening of third day and subsequent samples were sterile .in 20 cc.
Note.—This experiment is interesting on account of the preponderance of lactis æerogenes from
•start to finish showing that these organisms are to be looked upon as one of the fairly resistant ones.
Note also the gradual increase of bacillus cloacm as the experiment proceeds. Bacilli Nos. 100 and 67
were present at the beginning and persisted for a considerable length of time ; unfortunately the expe-
riment was spoilt by our not taking; sufficient samples on the third day so as to observe the las+
surviving organisms.
Experiment No. 26.
Emulsion before exposure contained .. 5 coscoroba, 1 lactis æaerogenes, 4 grunthal.
After 1 hour's exposure showed
... 4 coscoroba, 5 grunthal, 1 acidi lactici.
, ,
2 hours'
,,
,,
... 2 lactis 4 grungrunthal, 1 coscoroba, 2
No. 75.
.
3
... 1 coscoroba, 7 grunthal, 1 cloacae, 1 No. 75.
,,
,, 5 ,,
,,
,,
... 7 grunthal, 2 cloacae, 1 coscoroba.
.
Noon of the second day „
... 3 grunthal, 2 cloac, 2 lactis æærogenes, 2 cos-
coroba, 1 No. 67.
Evening of the second day showed ... 3 coscoroba, 3 grunthal, 1 No. 67, 1 No. 101,
1
No.
75.
Evening of the third day showed ... 10 grunthal.
Fourth day showed
... 10 grunthal.
Fifth day and subsequent samples proved sterile.
Note.—The appearance, towards the end of the exposure, of bacillus cloacae and final survival of
bacillus grunthal ; appearance and disappearance of lactis æærogenes ; organism No. 75 appeared in
'this experiment and persisted till fairly near the end.
Experiment No. 27.
Emulsion before exposure contained.
8 coscoroba, 1 cloaca, 1 grunthal.
After 1 hour's exposure showed
... 1 lactis æerogenes, 7 cloacw, 1 coscoroba, 1
•
grunthal.
,,
,,
, ,
,, 2 hours' ... 1 coli communis, 2 coscoroba, 5 grunthal, 2
•
cloacae. , ,
3 ,,
, , ,,
...
5 grunthal, 4 coscoroba, 1 lactis æærogenes.
5 „
10 coscoroba.
,,
Noon of the second day
,, ... 2 acidi lactici, 3 lactis æærogenes, 4 No. 101.
At 5 P.M. second day
,,
• . . 6 coscoroba, 3 1 neapolitanus.
Noon of third day
,,
... 4 grunthal, 5 acidi lactici, 1 lactis æ rogenes.
Evening of third day
,,
... 3 lactis æmrogenes, 5 No. 101, 2 acidi lactici.
•
Evening of fourth day
,,
... ... 1 grunthal, 2 acidi lactici, 3 No. 33, 1 No. 67,
Evening of the fifth day showed ... 2 cloacae, 8 coscoroba,
Evening of the sixth day „
.., 9 coscoroba, 1 cloacae.
Evening of the eighth clay „
... 10 coscoroba.
Subsequent samples proved sterile in 20 cc.
\ Note.—Slowness of the disappearance of the organisms ; very late appearance and disappearance
of rare organism No. 33 belonging to MacConkey's group 2 ; usual sequence .of appearance and
disappearance of organisms with the exception of coscoroba which persisted till the end.
Experiment No. 28.
Emulsion before exposure contained.
7 grunthal, 1 cloacw, 2 No. 101.
After 1 hour's exposure showed
... 2 cloacm, 3 coscoroba, 1 lactis
æærogenes, 2
No. 67, 1 No. 101.
•