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EXPLANATION OF PLATE II.
Figure 1.—Enlarged parasites showing longitudinal division and multiple segmentation. Stained by
Giemsa's stain. Magnification 1,000 diameters.
(a) Large bean-shaped parasite with a fully developed flagellum which ends in a
chromatic dot between the macro-and micronucleus.
(b) Similar parasite showing longitudinal division into two smaller flagellates.
Note as division has taken place before the flagellum has fully developed, it has
split throughout its length.
(c) Two large parasites showing various stages of longitudinal division.
(d) Rosette of four flagellates showing the division of the protoplasm.
(e) Large rosette containing five macronuclei and six micronuclei ; four flagella have
passed out from one side and a fifth is developing in the centre.
Figure 2.—Spindle-shaped flagellates. Stained by Giemsa's stain. Magnification 1,000 diameters.
(a) Spindle-shaped flagellate dividing, the second flagellum has split off from only a
part of the original one.
(b) Similar cell showing unequal longitudinal division.
(c) Two long parasites formed by the division of a large spindle-shaped flagellate.
(d) Division of a large spindle-shaped flagellate into two smaller ones.
(e) Two similar parasites ; the micronuclei are still close up to the macronuclei and
are elongating indicating the cells are about to divide.
Figure 3 .—The rosette and its division. Stained by Giemsa's stain. Magnification 1,000 diameters.
(a) Two large flagellates detached from a rosette containing four parasites.
(b) Four elongated flagellates from a rosette of six parasites.
(c)
Large rosette containing two large nuclear masses, eight micronuclei and six flagella.
In these rosettes the majority of the flagella usually pass out fron one side of the
cell.
(d) Two long flagellates from a rosette of six parasites.
Figure 4.—Four long flagellates, in one there are a few granules at the posterior end. Stained by
Giemsa's stain. Magnification 1,000 diameters.
Figure 5.— Appearances seen towards the end of development. Stained by Giemsa's stain. Magnifica-
tion 1,000 diameters.
(a) Two small flagellates attached by their posterior poles ; one of the cells is about
to divide again.
(b) Large oval flagellate with a smaller cell splitting off.
(c) Small elongated flagellate with two chromatic granules at the posterior end.
(d) Two attenuated flagellates from the outer side of one of which a thin parasite is
splitting off.
(e) Small oval parasite showing the early formation of the flagellar vacuole ; this
parasite had been in the midgut of the bug for five days.
(f) Larger oval parasite from the same bug. There was no appearance suggesting the
development of the flagellum.
Figure 6.—Flagellates showing multiple segmentation. Stained by Giemsa's stain. Magnification
1,000 diameters.
(a) Enlarged round parasite showing the development of the flagellum recovered from
a bug five days after being ingested.
(b) Small flagellate showing multiple division which results in the formation of small
irregular flagellates.
( c) Free attenuated flagellate.
(d) Two small flagellates, the result of the division of a larger oval or spindle-shaped
cell ; each daughter cell shows evidences of multiple segmentation.
(e) Small irregular flagellate containing two macronuclei and three micronuclei.
(f) Two small flagellates attached by their posterior poles.
(g) Long flagellate showing evidences of irregular division.