NationalLibraryOfScotland/medical-history-of-british-india huggingface dataset:
34
Sample IX—Date, 4th September—Depth of
water, 17' 7"--Rainfall, 34 inch on 3rd.
C o l o n y
N o .
Lactose Sacchar ose
I n d o l
P & V r e a t o p n
I ndol
M o t i l i t y
Bacillus. Gr oup
1 — + — — — II B. Coli Communis' etc.* G r o u p
(a) Bile salt glucose, medium.
2
I B. Grunthal.*
3
I
Do.
4
I
Do.
tube 20 cc. of water taken
..
. .
5
6
—
—
+
+
—
—
—
—
—
—
—
—
I I B. Coli Communis, etc.*
I I
Do.
,,
I
2 tubes 10 cc.
11
..
2 ..
7 — + — — — — II
Dc.
4 „ 5 cc.
,,
. . 4 ..
8
9
I B. Grunthal.*
1
Do.
5
1 cc.
5,
. •
5
. . 10
I
Do.
1
•
•
.
cc
5 „ .
17
.
. .
11_
12
— + — — — — II B. Coli Communis, etc.*
I B. Grunthal.*
5 „ •01 cc.
„
• • ..
13 — — -- - — I
Do.
14 — + — — — — II B. Coli Communis, etc.*
15
I B. Grunthal.*
16
I
I
Do.
(b) Fœca bacilli in 1 cc. and upwards.
17 +
IV B. Coscoroba.
18 +1
IV
Do.
19 -- - — — — I B. Grunthal.*
20 —I— — — — — I
Do.
Sample X—Date, 12th September—Depth of 1 + — —I— + — IV B. Cloacæ
Group
water, 16' 2"—Rainfall, 1 .03 inch on
(a) Bile salt glucose, medium.
11th.
2
3
4
5
+
+
+
+
—
—
—
—
——
+
+
+
—
—
—
IV
IV
IV
IV
Do.
B. Coscoroba.
B. Cloacæ.
Do.
, ,
, ,
+
1 tube 20 cc. of water taken ..
2 tubes 10 cc.
,,
••
4 „
5 cc.
55
• •
5 „
1 cc.
,, •
•
1
2
4
2
6
..
7
. •
8
"
9
3
10
+
+
+
— --
-
-
-
-
-
-
-
-
-
+
+—
+—
++
+—
— I V
III
III
III
III
Do.
No. 73.
Do
No. 75
No. 73.
5 „
.1 cc.
,,
5 „ .01 cc.
,,
(b) Fœcal bacilli in 3 cc. and upwards
-.
5
11
5
12
+
I
I
B. Grunthal.*
Do.
Sample XI—Date, 22nd September—Depth of +— ---- +,+ IV B. Cloaca.
Group
water, 16' 6"—Rainia II, nil.
2 +— ------ + + IV
Do.
3 +
+ + IV
Do
(a) Bile salt glucose, medium.
4 + — — — + + IV
Do.
+
5 + — -- + + IV
Do.
1 tube 20 cc. of water taken ..
6 +
7 +
— — —
— —
+
+
+
+
IV
Do.
IV
Do,
1
•
2 tubes 10 cc.
, , ..
2
. . 8 + — — — + + IV
Do.
4
5 cc.
,,
• •
1
. . 9 + — — — + + IV
Do.
5 „
1 cc.
,,
• •
5 ,, 1.0 + — — — + + IV
1)o.
5 „ • 1 cc.
11
..
5
•01 cc
(b) Anal bacilli in .1 cc. and upwards.
annnnn••....n
4
. ,
1 11 +
12 +
—
—
—
—
—
—
+
+
+
+
IV
Do.
IV
Do.
Sample XII—Date, 28th September—Depth of 1 + + —— — — III B. Neapolitanus.
I
Group
water, 1 6' 6"—Rainfall, 25; inch
2 + + —!-- -- — III
Do
,, I
(a) Bile salt glucose, medium.
3
4
5
+
+
+
+
+
—
—
— : —
— — —
—
— 1 — — —
II (
III
Do.
.
IV B. Coscoroba.
, ,
I I
,,
—
1 tube 20 cc of water taken
• '
. 6
7
+
+
+
+
—— — —
—— — —
III B. Neapolitanus.
III
Do.
1 •
2 tubes 10 cc.
,,
.. 2 ..
8 + + — — — — III
Do.
4 „ 5 cc
,,
4
•
9 + + - 1- - - m
Do.
„
• •
5 „ 1 cc.
,
,
.
1
•
5
cc
..
5 ,,
5
I 0
1.1
+
4-
—
+
— I— —
- !- 1-
—
-
I V B. Coscoroba.
III B. Neapolitanus.
5 „ •01 cc.
..,
• • ..
5
12 -I- + +
— III No. 73.
(b) Fœcal bacilli in .1 cc and upwards
36
Sample XIII—Date, 9th October—Depth of
water, 16' 6 "—Rainfall, nil.
I
c o l o n y !.. ) S a c c l i e r o s e
a
S
it.
ulc
it
d o n
A
d
s
ge
V
li oto
M
p
g
G
Bacoillus.
G r o u p R e m a r k
1 -1- + -1- — + — iii No.
Group I
(a) Bile salt lactose, medium.
+ — 2
3
+
+
+
+
+ + —
—
—
—
—
—
III
III
1-
ææææ or rhi-
nosoleromatis.
,
II
1 tune 20 cc. of water taken
7
1
4 + + + — + —
I I I
No. 67.
Do.
,,
III,
2 tubes 10 cc.
,,
.., , 2
4
..
• •
5
7 6 7
+
t +
+
+
+
+
+
— +
— +
— —
—
—
—
III
m
III
.)
Do.
B. Pneumonia or rhi-
,,
Iv,
5 „ 1 cc.
,,
• • 5
8 + + + — + —
I I I nosoleromatis.
9 + + + — 4- — III No. 67.
-1
5 „
cc.
, ,
. . .
5 „ .01 cc.
,,
..
2
..
3
5
io
11
12
+ +
--
+
+
--
+
+ I—
—+—
+
— +
—
—
iv I I I
III
B. Cloak.*
No. 67.
(b) fæcnal bacilli in 1 cc. and upwards.
Sample XIV—Date, 16th October—Depth of
1 + — + — + — IV
B. Lactis æro genes
capsulatus.
Group I,
water, 17 '—Rainfall, nil.
2 + — + — + — IV
Do
II,
(a) Bile salt lactose, medium.
(
1 tube 20 cc. of water taken ..
3
4
5
6
7
+
+
+
+
+
+
—
+
_
+
—
+
+ +
+
—
—
—
_
—
+
+
+
+
+
—
—
— _
—
I I I
I V
I V
III
iv
III
III
No. 73.
B. Laotis
ærogenes
„ III,
or capsulatus.
No. 67.
,, IV
B. Lactis
ærogenes
or capsuiatus.
No. 67.
+ —
1 • •
2 tubes 10 cc.
,,
.. 2 .. 8 + + + — + —
Do.
4 „
5 cc.
, , . .
1 3 9
10
+
+ +
+
—
—
—
4- + —
—III I I I
Do.
N0.73.
5 „
1 cc.
,,
..
..
5
I
5 „
.1 cc.
, ,
, ,
..
5
5 „ • 01 cc.. ,,
(h) Fæcal bacillar in 10 cc. and upwards.
.. 5
Sample XV—Date, 24th October—Depth of
1 -I- + + + + — III Oxylocus pernioiosus. Group I,
water, 17' 3 "—Rainfall, to 23rd, 5'5 inches.
2 + + -I- + + — III
Do.
, ,
II,
(a) Bile salt lactose, medium.
3 + + + +
+
+
+—
-- III
III
Do.
Do,
„ III,
„ IV,
+ —
4
5 + + + + + — III
Do.
1 tube 20 cc. of water taken .. 1
6
7
+
+
4-
-I-
+
+ +
+
+
—
—
III
III
Do.
Do.
2 tubes 10 cc. ,,
2 .. 8 -I- + + + + -- III
Do.
4 ,,5 cc.
,, . , 4
9 + — + — + — I V B. Laotie aerogenes or
capsulatue.
10 +
+—+— V
Do.
5 „ 1 CC.
,,
• 7
5 ..
5 „
•1 CC.
,,
••
••
5
5
. 01 cc.
-,
,,
•
(b) Fecal bacilli in 1 cc. and upwards.
. . 5
B. Pneumonæ or rhi- Group I
ample XV1—Date, 3rd November—Depth
water, 1 ' 9" *—Rainfall, to 3rd
12
inches.
43
of
Nov.,
1
2
3
4
+ +
+ —
+ —
+
+ —
+ —
+ --
+
—
—
+ +
--
—
—
III
I V
IV
IV
noscleromatis.
No. 101.
B. Laotis ærogenesor
c a p s u l a t u s .
Do.
I I
, ,
I I I
,, Iv,
„ IV,
(a) Bile salt lactose, medium.
5
6
+
+ +
+ —
+ —
—
+
—
—
III
III
B. Pneumonia, or rhi-
nosclerpmatis
*pump
going
1
tube 20 cc. of water taken ..
'2 tubes 10 cc.
,,7 7
1
2
..
..
7
9
— —
— —
— —
— —
—
—
—
—
—
1 ,
I
I
B. Grunthal.
Doo..
the ti
4 „ 5 cc. ,,
• • 4 .. 10 -- -- - - -
o.
5 „ 1 cc.
,, .. •
•
5 ..
5 ,.
1
cc.
,,
• 0
5 „ • 01 cc.
77
• 7
5 ..
5
. .
(b) Fecal bacilli in • 01 cc. and upwards.
7
Chemical Analysis of King Institute Well water.
Parts per 100,000.
Sample
...
...
Date
...
...
...
...
Colour
...
...
...
Smell
... ... ... ...
Total solids
...
...
...
Temporary hardness
...
Permanent
do.
...
Total
do.
...
...
Chlorine ... ... ...
Free ammonia
...
.
...
Albuminoid ammonia
...
Oxygen absorbed (Tidy's proces s)
Nitrates (N 2 O 5) ...
...
...
Nitrites
...
...
...
...
Sulphates ...
...
..
...
Phosphates
...
...
..
Iron, poisonous metals ...
...
...
...
. . .
...
...
...
. . .
. . .
...
. . .
...
...
...
...
...
...
...
...
...
. . .
. . .
...
...
. . .
...
...
No. 19
26-11-08
Clear.
None.
38.000
14.500
0.500
15.000
4 . 6 1 5
Trace.
0.003
0.007
Trace.
Nil.
Present. .
Nil.
Nil, Nil.
No. 23
5-1-09
Clear.
None.
47.600
16 . 500
0.500
1 7 . 0 0 0
6.035
Trace.
0 . 0 0 3
0 003
Trace.
N i l .
Present.
Nil.
Nil, Nit.
No. 27
4-2-09
Clear.
None.
4 5 . 8 0 0
16.000
0'500
1 6 . 5 0 0
5.680
0.004
0.007
0.010
0.135
Nit.
Present.
Nil.
Nil, Nil.
No. 28
23-2-09
Clear.
:None.
42.400
14.500
1.500
16 000
4.615
0 00l
0.003
0.006
Trace.
Nil.
Present.
Nil.
Nil, Nil.
No. 33
30-3-09
Clear.
None.
40 400
15 000
1.000
16.000
4.615
0.006
0.007
0.006
Trace.
Nil.
Present.
Nil.
Nil, Nil.
Conclusions.—(1) In common with most other water-supplies, including rivers,
l
lakes, springs, etc., bacillus cloacæ would appear to be the predominating organism
ii
n a well after prolonged drought.
(2) Even a small downpour of rain, after a prolonged spell of dry weather, has
a very marked influence in increasing the number of fœcal bacilli in wells situated in
porous soils.
(3) That early showers following on a long period of hot weather, have caused
an increase in the number of soli communis in the well of the King Institute.
Further investigation is necessary before it can be slated that this is true of all wells.
It is not by any means certain where these soli communis come from, but it is
unlikely that they come from the surface of the ground, and probable that they
remained alive in the lower layers of the soil.
(4) That after heavy downpours of rain, in consequence of which it is certain
that surface contamination has penetrated deep into the layers of the soil, a mixture of
fœcal organisms is usually found in the water obtained from wells. Coli communis
appear to be present after early rains, but disappear as the rains become more plentiful.
(5) Plentiful rains improve the quality of sub-soil water after the contami-
nated surface water has run off the land.
(6) Shallow wells are a most unsatisfactory source of water-supply, especially
i
if they are situated in a highly porous soil, and unless the greatest possible care is
exercised in protecting a large space of ground in the immediate vicinity from all
chances of pollution.
10
38
Sample XVII—Date, 7th November—Depth of
water, •15
2 inch.
9"—
.
Rainfall, 4th and 6th Nov
ember
c o l i n y
N o
s a c c h a r o s e
Dui c l t
A d o n i t
i n u b r a
Inunts
V a g e s a n d p r o c k
G r o u p s
Bacillus.
G r o u p
Remark
(a) Bile salt lactose, medium.
+ I —
1 + + — — + — III No. 73.
Group I
1 tube 20 cc. of water taken
..
2 tubes 10 cc.
, ,
. .
..
2
3
4
5
-I-
+
+
+
+
+
—
+—
—
—
+
—
—
—
—
+
+
+
+
—
—
—
—
Ill
I11
III
Iv
,,
,,
„
B. Lactis æærogenes or
,, II
,, III
,, IV
1
2
4 „
5 cc.
,,
. . 4 ..
6 + + — — + —
I I I No. 73.
5 „ 1 cc. ,,
..
3 2 7 f — — — + — V B. Cloaca.
5 ,1
cc.
,,
• •
. . 5 8
9
+
+
+—
+—
—
—
+
+
—
—
III
11.I
No. 73.
5 „ •01 cc.
, , ..
(b) Fœcal bacilli in 1 cc. and upwards.
• •
5 10 + + — — + — III
, ,
, ,
Sample XVIII—Date, 16th November—Depth of 1 + —
+ + — Iv B. Lactis ærogenes or
.
Group
water, 15'—Rainfall, nil.
2 + +
+ - III No. 67.
„ I I
(a) Bile salt lactose, medium.
+ —
3
4
+
+
+.++—
—
+
±
+—
-- III
Ili
, ,
,,
„III
„IV
1 tube 20 cc of water taken
.. . . . .
1
5 + — + — + — IV B. Lactis æ rogenes or
capHulatus.
2 tubes 10 cc.
,,
..
1 1.
6 + — — — + — IV .B, Cloaca.*
4 „ 5 cc.
,,
1 3
7
8
+ —
+ —
— —
— —
+
+
—
—
IV
IV
Do.
Do.
5 ,,1 cc.
,,
.. 5
9 + — — — + — IV
Do.
5 „
.1 cc.
,,
• • . . • 5 10 + + — — — — III B. Neapolitanus.
5 „ • 01 cc-
, , ...
(b) Fœcal bacilli in 20 cc. and upwards.
• •
5
Sample XIX—Date, 23rd November—Depth of
1 + + ,+ — + — lll No. 67.
Group I
water, 15'—Rainfall, -35 inch.
(a) Bile salt lactose, medium.
1 tube 20 cc.. of water taken ..
2
8
4
5
-1-• +
+ +
H- +
1+ +
1
+ —
+ --
+ -
+ —
+
+
+
+
—
—
-
—
ill
111
III
III
,,
,
„
,, II)
„ III,
„ IV •
+ —
• •
1
2 tubes 10 cc.
..
„
1
6 1-1- + + — + — III
„
4
5 cc.
15
,.
..
1 3
7 I + +
n
+ _ -
4: 1- — Ili
„
5
1 cc.
..
,,
.. 5
8 1+ + + _ + — III
„
5
„
1 cc.
• •
.. •
'5
9 1+ + + _ + — III
5 „ • 01 cc.
. .
(b) Fœcal bacilli in 20 cc. and upwards.
. .
r,
,
10 + + + — + — III
Sample XX—Date, 1st December—Depth of
1
I B. GrutIthal.''
Group I:
water, 15'—Rainfall, 2 .86 inches.
1
2 + — — — —. + IV B. Coscoroba.
:, Il
3
+ 1 B Grunthal.
„ III
(a) Bile salt lactose, medium.
-I- I —
4 +
IV I3. Ccscoroba.
: ) 11
5 +
IV
Do.
1 tube 20 cc. of water taken
..
1. • -
ti
I B. G:runtlial.*
2 tubes 10 cc.
,
. .
4 .,
5 cc.
3)
• .
4
..
..
7 + I
8 + — — — —
9 +
—
IV
IV
IV
B. Coscoroba.
Do.
Do.
5 „ 1 cc... 13
5 ,, 1 cc.
,,
.. •
5 „ •01 cc cc.
,,
• •
5
.. •
..
..
5
5
10 +
Do.
(6) Fœcal bacilli in 1 c c. and upwards.
,
39
Sample XXI— Date, 8th December—Depth of
water, 13'—Rainfall, '18 inch.
c o l o n y
N o s a c c c h a r o s e
D e l u t e
A d o n i t
I n u n l i o n V o r k s a n d p r o s k
M o d i l
y
G r o u p s
Bacillus.
G r o u p a n d R e m a r k s .
1(a ) Bile salt lactose, medium.
1
I
I B. Grunthal. *
Group I, 2.
2 +
IV B, Coscoroba.
„ II, nil.
1 tube 20 cc. of water taken
1
. . 3
I B. Grunthal. *
„ III, nil.
2 tubes 10 cc.
,, ..
2
..
4
5
+
+
IV
I V
B. Coscoroba.
Do.
,, IV, 8.
4 „
5 cc.
, ,
— 4 .. 6 +
I
.1)o.
5 „
1 cc.
:,
5
. .
7 8 +
+
IV
IV
Do.
Do.
5 „ . .1 cc.
,, ..
5 .. 9 +
1V
Do.
5 „ • 01 cc.
, ,
. .
(b) Fœcal bacilli in .1 cc. and upwards.
..
5 10 + i
IV
Do.
.....—
S a m p l e
X X I I — D a t e ,
1 8 t h D e c e m b e r — D e p t h o f 1 + — — — — — IV B. Coscoroba.
Group I, nil.
water, 12' 6".—Rainfall, nil.
2 -I-
IV
Do.
„ II, nil.
a)
Bile salt lactose, medium.
3 +
IV
Do.
„ III, nil
—
4 + — — — — — IV
Do.
„ IV, 10.
1 tube 20 cc. of water taken
..
1 . .
5 +
IV
Do.
2 tubes 10 cc.
,,
.
. . • 6 +1
IV
Do.
4 , 5 cc.
.. • 4 .. 7 +
+
IV
IV
Do.
Do.
5 „ 1 cc.
)1
. .
3 2 9 +
IV
Do.
5
„ •1 cc,
,.,
• •
. . •
5 10 + — — — — — IV
Do.
•
_
D
"
. 01 cc.
,,
.. . .
5
(b)
Fœcal bacilli in 1 cc. and upwards.
Sam ple XXIII—Date, 3rd January—Depth of 1 — — + — — I NO. 9.
Water, 16' 2"—Rainfall on 28th December—
th January, 6 .61 inches.
II (a) T,.tal colonies on agar at 37°-180.
2
3
—
— —
— —
+
+
+
—
—
+
+
—
—
—
—
I
1
;
,,
IV,.' (b) Bile salt lactose, medium.
+ — 4 --= — + — + — — I
„
1 tube 20 CC. of water taken. ..
f- i
6
+ —
+
+ --
—
+ —
—
_
—
i v
III
B. Lactis ærogenes or
capsulatus.
No. 67.
Group I, 6.
„ II, ntl.
1 • •
2 tubes 10 cc.
•,
• ..
4 „
5 cc.
",
• •
2
4
. . • 7
+
+ +
+
— —
+
+ — — CII. No. 75.
,,III, 2.
IV, 2,
.• 8 — — — — — — — I B. G.runthal.*
„
5„
1 cc
"
'
1
4 9 _ _ _ ____ _ ____ _ I
Do.*
5 „ -1 cc.
,,
. . . .
5 1.0 +- - - -- IV B. Cloacæ.
5
. 01 ec.
..
»
»
. . . .
(b) Fœcal bacilli in 5 cc. and upwards.
S a m p l e
X X I V — D a t e ,
l a h J a n u a r y — D e
II 15' 3"—Rainfall on 5th-January,
111 Total
.th of
0'61 inch.
1
2
+ +
+ +
—
—
—
—
— — —
— + —
III
III
B. Neapolitanus.*
Do.
Group I, 2.
„ II, 1.
il
colonies on agar at 37°-130.
1Bile salt lactose, medium.
+ — 3 + - - -+— III
Do.
„ III, 7.
4 + + — — — + — III
Do.
„ IV, nil.
1 tube 20 cc. of water taken
.. 1 .. .. 5 + + — — — + — III
Do.
. 2 tubes 10 cc.
2 .. 6
'1
. . •
+ -4- — — — + — III
Do.
4 „ s cc.
»
.. 4 . . 7
ti
5 ,, 1 cc.
»
.. , , 5 8
+ + — — — + —
+ —
Ill
1
Do.
B. Grunthal.*
5
'1 CC.
. .
5 9
..
+ — I
Do.*
,)
„ .0 1 CC.
,
10
•
•
. ,
5
— + — — — + — II B. Coll commmunis, etc.
( b )F
œ c a l b a c i l l i i n 5 c c .
a n d u p w a r d s .
rc
40
Sample XXV—Date, 19th january Rainfall,n i l .
1 9 t h j a n u a r y R a i n f a l l , , ,
1 9 t h J a n u a r y — R a i n f a l l ,
nit.
C o l o n y
N o
S a c c h a r o s e Du l c i t A d o n i t
Up g r s p r
pr os k
I n d o l M p t i l i t y
G r o u p s
Bacillus.
Group and
Remarks.
(a) Total colonies on agar at 37 °— 360.
( b ) Bile Salt lactose, medium.
+
1 — — -
— + — I B.Grunthal. *
Group I
1 tube 20 cc. of water taken ..
1
2
3
—
+
— — — — — —
+—
+—
I I
III
B. Coli communis etc.
,,
II,
, ,
I I I ,
2 tubes 10 cc.
. .
4 — + _ — — — II B Coli communis etc
,,
IV
4 „ 5 cc.
,, ..
5 „
1 cc.
, ,
4
5
..
• •
5
6
7
— — —
+ +
— —
—
—
—
—
—
—
—
—
+ —
— —
— —
I
III
I
B. Grunthal.
B. Neapolitanus.
B. Grunthal.*
5
. .I cc.
.. 2
8
+ — I
D o .
,,
9 + — — — — + — IV B. Coscoroba..
5 ,,
.0 1 c c .
, ,
. .
(c)Fœcal bacilli in 1 cc. and upwards.
. . . .
5
10 +
IV
Do.
_
Sample XXVI—Date, 26th January—Depth of
water, 15'—Rainfall nil.
—
1
2
3
—
+
—
—
+
+
+
+
—
—
—
—
+
+
—
+
+
+
—
—
—
I
I I I
II
No 9.
No. 67.
B. Coli communis. etc.*
Group I,
II,
(a) Total colonies on agar at 37° —220.
4 — + + — + + — 11 No. 38.
" III
( b ) Bile salt lactose, medium.
1 tube 20 cc. of water taken ..
+
5
6
7
+
+
+
+
+
+
—
—
—
—
—
—
—
—
—
+
+
+
—
—
—
I I I
III I I I
B. Neapolitanus.
Do.
Do.
„ IV,
1
2 tubes 10 cc...
,,
2
. .
.. 8 + + — — — + — III
Do.
4 „ 5 cc... ,,
4
..
9 + — — — —
+ — IV B. Coscoroba.
5 „ 1 cc... , ,
5 . . . 10 + + — — — + — III B. Neapolitanus.
5 „ • 1
c c , ,
,,
5
. .
5 ., .01 cc... . ,
(c) Fœcal bacilli in .1 cc. and upwards.
..
5
Sample XXVII,
—
Date, 3rd February-Depth 1
—
+
— — —
+
—
II B. Coli communis, etc.* Group I,
of water in the well, 15'- Rainfall,
( a) Total colonies on agar at 37°—690.
nil.
2
3
+
+
+
+
—
—
—
—
—
—
+
+
—
—
III
B. Neapolitanus.
III
Do.
„ II,
„ III
( b ) Bile salt lactose, medium.
+ — 4 + + — — — + + III
Do.
„ IV,
1 tube of 20 cc. of water taken. 1 . . 5 + — —
— + + IV B. Coscoroba.
2 tubes of 10
of
tubes of 10.
c c
2 t u b e s o f 1 0
of
tubes of 10.
c c
, ,
2 . . 6 +
— — — + —. III B. Neapolitanus.
4 „
5 cc.
, ,
5
1 cc.
,,
,,
4
5
. .
..
7
8
9
+
+
—
+
—
—
—
—
—
—
±
+
+
—
—
—
IV B Coscoroba.
III B. Neapolitanus.
I B. Grunthal.*
5 ,,
•1 cc.
, ,
2 3 10
+ — I
Do.
5 . 77
.01 cc.
,,
( c) Fœcal bacilli in 1 cc. and upwards.
5
Sample XXVIII—Date. 11th February—Depth,
16' 6"—Rainfall, nil.
1 — — — — — + + I B. Grunthal.
Group I
2 + — — — — — — IV B. Coseoroba.
,, II
(a ) Total colonies on agar at 37°—480.
3 — — + — — + + I B. Acidi lactici.
„
I I I
( b ) Bile salt lactose, medium.
4
+
—
L V B. Coscoroba.
77
IV
1 tube of 20 cc. of water taken.
+
______ ___
..
5
6
I
+ I
B Grunthal.
Do.
2 tubes of 10 cc.
, ,
4 „
5 cc.
,,
5 ,,
1 cc.
, ,
2
4
5
. .
7
8
+ —
+ —
IV
IV
B. Coscoroba.
Do.
5
,, •1 cc.
, ,
5 „ .01 cc.
,,
(c ) Fœcal bacilli in 1 cc. and upwards.
. . . .
5
5
9
10
+ —
+ —
— —
— — — — ——-
IV
IV
Do.
Do.
41
Sample XXIX—Date, 15th February—Depth,
15' 2"—Rainfall, nil,
C o l o n y
N o .
S a c c h a r o s e
Dul i ci t .
Adonit.
I n u l i n .
Vogesor Prosk.
M o t i l i t y .
G r o u p .
Bacillus.
Group and
R e m a r k s .
(a) Total colonies on agar at 37°-390.
1 — + — — — + — II B. Coll oommunis, etc.' Group I, 6,
(b) Bile salt lactose, medium
+
—
2
3
+ --
+—
I
I
B, Grunthal.*
Do.
„ II, 1.
4 + +
III B, Neapolitanus.
III, 1
.
1 tuba 20 cc. of water taken,
1
. •
5 — — — — — + —
I B. Grunthal.*
,, IV, 2.
2 tubes 10 cc,
15
• •
6
+ —
I
Do.
4 „
5 cc,
',
5 „
1 cc.
, ,
4
5
. .
..
7
8
—
+
— — — + + —
I
IV
No. 6,
B, Coscoroba.
5 „
1 cc.
, ,
5 „ • 01 cc.
,,
2
• •
3
5 10
9
+ — — —
+ —
—
I
IV
B. Grunthal.*
B. Coscoroba.
(c) Faecal bacilli in 1 cc, and upwards.
Sample XXX—Date, 1st March 1909—Depth,
+ —
— + + IV B. Coscoroba.*
Group I, 4.
16' 6"—Rainfall, nil,
2 + —
— + — IV
Do.
„ II, nil,
(a) Total colonies on agar at 37°-79.
3 + —
— + — IV
Do.
„ III, nil.
(b) Bile salt lactose, medium,
4 + —
— +— IV
Do.
„ IV, 6.
1 tube 20 cc, of water taken ,,
' •
5
+
IV
Do.
2 tubes 10 cc,
,, • •
2 • •
6 + - _ _
+ + IV
Do.
4 „
5 cc.
,,
..
4 • •
7
I B. Grunthal.*
5 „
1 cc.
,,
..
5 ..
8 —
+ — + I No, 6.
5 „
1 cc.
,,
2
3
9
+ — I B, Grunthal.*
10
I
*
5 „ • 01 cc,
,, ,,
..
5
Do.
(e) Faecal bacilli in 1 cc, and upwards,
Sample XXXI—Date, 9th March 1909—Depth, 1 + — — — + + — IV B, Cloacæ
Group I, nil.
16' 6"—Rainfall, nil.
2 + — • — — + + — IV
Do *
„ II, nil.
(a) Total colonies on agar at 37°-440,
—
3 + — — — + + — IV
Do. *
„ III, 3.
(b) Bile salt lactose, medium.
+
1 + — — — + + — 1V
Do, *
,,IV, 7,
1 tube of 20 cc. of water taken.
1 ..
5 + — + — + + _ IV B. Ls zerogeues or
capsultaus.
2 tubes of 10 cc.
,,
4
,,
5 cc.
,,
5 „
1 cc,
,,
5
• I cc,
2
3
••
. .
..
1
5
5
6
8
+ —
+
-
+
+
-
-
—
—
-
+
+
+ _
+ —
+—
IV
IV
III
Do.
B. Cloac. *
No, 75.
5 „
• 01 cc.
,,
5
9 +
- -
+— III
Do.
10 + + — — + + — III Do.
(c) Faecal bacilli in 5 cc. and upwards.
Sample XXXII—Date, 19th March 1909—
Depth 16' 4"—Rainfall, nil.
1 +
IV B. Coscoroba.
I, n
Group , a,
(a) Total colonies on agar at 37°-86.
2 + + —
— + — III B. Neapolitanus.
„
II, nil.
3 +
IV B, Coscoroba.
„ III, 3.
(6) Bile salt lactose, medium,
+
4 +
IV
Do
i ,, IV, '7.
1 tube of 20 cc. of water taken,
1 • - 5 4-
IV
Do.
2 tubes 10 cc,,
4 „
5 cc
• •
. .
6
7
8
+
+
+
IV
IV
IV
Do.
Do,
Do.
5 „
1 cc,
,,
• •
5
9 + + -
— + — III Neapolitanus,
5 ,, .1 cc.
,,
..
5 10 + + — — + + — III No, 75.
5 „ 01 cc.
,,
(c) Faecal bacilli in 10 cc, and upwards,
. .
5
I
11
42
Sample XXXIII—Date 30th March—Depth of
water 16' 0—Rainfall, nil.
C o l o n y N o . Saccharose. Du l c i t . A d o n i t . I n u l i n . V o g e s o r
P r o s k .
I n d o l .
Mo t i l i t y
G r o u p .
Bacillus. G r o u p s
a n d
R e m a r k s .
(a) Total colonies on agar at 37º—78.
.
(b) Bile salt lactose, medium,
2
1
— + — — — + —
I
II
B. Grunthal. *
B. Coli communis*
Group I,
,, II,
1 tube of 20 cc. of water taken ..
2 tubes of 10 cc.
,,
..
4
5
3
—
—
+ —
—
_
—
—
—
+
+
—
—
I
II
I
B, Grunthal. *
B. Coli communia. *
B, Grunthal,*
,, III,
„IV,
1
2
• •
..
4
5 'cc,
. . • •
4
6 — — — — — + —
I
Do,
5 ,,
1 cc,
. .
, ,
1
4
_ _ _ _ _
I
Do,
5 „
,1 cc.
,,
• •
• •
5
9
—
+
I
I
Do.
Do.
5 ,,
•01 cc.
..
, ,
(c) Fœcal bacilli in 10 cc. and upwards.
• •
5
1
101 0 — + — — — + — II B Coli communis.*
44
Series No. 5.—In consequence of the disappointing results given by series Nos..
3 and 4, three separate samples of fœces were plated out on the solid medium direct in
the usual way, 20, 15, and 15 colonies from each plate being taken. These results.
show that the method of using separate samples, with or without, bile salt broth,
was infinitely superior to that of employing mixtures. The reason for the failure of
mixtures, is due to the one weakness of our method, viz., that only organisms that
predominate numerically are obtained on the plates ; when mixtures are made use of,
the chances of getting rare organisms are proportionately reduced. For further
discussion of this point see Chapter VIII.
Concerning series Nos. 6, 7, 8 and 9 no remarks need be made. They all followed
the standard method.
The results obtained have been tabulated in tables VII (a), (b), (c).
Tables (a) and (b) deal the percentage of bacilli in MacConkey's groups in each
of the nine series.
TABLE VII (a).
Human races. (Percentages.)
—
Series
No. 1.
Series
No. 2.
Series
No. 3.
Series
No. 4.
Series
No. 5.
Series
No, 6.
Series Grand
No. 9. average.
Average
without
series
3 and 4.
Group I
,..
...
... 46. 6
54
8 4 . 7
76.7
48
52
78
62.9
55.7
,,
II
...
... 25.3
35
.7
10
40
12
4
1 8 . 1
23.2
„ III
1 1 . 3
2
. . . 14
8
2
5.4
7.5
IV
...
...
...
16.6
8'9
1 4 . 6
13.3
2
28
16
14.2
14.3
, ,
TABLE VII (b) .
Cowdung. (Percentages.)
—
Average
Series Series Series Series Series Series Series Series Series Grand without
No. 1. No. 2. No. 3. No. 4. No. 5. No. 6. No. 7. No. S. No. 9. average. s e r i e s N o s . 3
and 4.
Group I
... 35. 3
58.3
70
86.6
52
36
40.8
36.8
4 2 . 7
50.9
43.1
„,, I I
. . .
8
13 6
...
...
18
17
3 . 7
1S.1
3.6
8.6
11.0
„ III
...
22
6.8
2.7
16
10
22.2
9.4
5.4
10.5
13.1
„ IV
...
34.6
21.7
27.3
13.3
14
37
33.3
40.6
49.1
30.1
32.9
Before going into the subject of the various micro-organisms isolated, it will be
well to dispose of the matter of the groups. MacConkey in his works on the bacterio-
logy of fœces (1905) gives the following percentages among 241 lactose fermenting-
organisms isolated from human fces :--
Our averages in 751 colonies.
Group No. I...
34 per cent.
55.7 per cent.
..
No. II
38
„
23.7
„
... ...
„
No. III
...
...
15
7.5
„
No. IV
...
12
,,
14.3
He goes on to say "only 4 out of the 241 were shown definitely to
belong to the bacillus lactis ærogenes type. Bacillus cloaca was not isolated in one
instance. The cane sugar fermenting bacilli numbered 65 while non-cane sugar
fermenting bacilli reached a total of 176 " (about 1 to 3).
The best and fairest figure for purposes of comparison is our " average excluding
series Nos. 3 and 4 " ; (series Nos. 3 and. 4 cannot be looked upon as satisfactory for the.
reasons stated above). Figures obtained from our results are placed beside those given.
by MacConkey to facilitate comparison. Note that the difference is not very marked.
Lactis ærogenes is not a common organism in human fœces in this country, but it.