58
•
After 2 hours' exposure showed
...
1 cloacae, 5 coscoroba, 4 No. 67.
,, 3 . ,, ,, ... 10 coscoroba.
9 acidi lactici, 1 grunthal.
,,
4
,, ...
,, 5 ,, ,, ... - No organisms.
Noon of the second day showed
••• 7 cloacæ, 1 grunthal.
Subsequent samples contained no organisms in 20 cc.
Note.—This particular sample is not of any great value ; bacillus acidi lactici persisted till
towards the end; bacillus No. 67 appeared during the course of exposure and disappeared fairly
rapidly ; the final result showed cloacæ persisting till the end.
•
Experiment No. 22—Cowdung.
Emulsion before exposure contained.
5 coscoroba, 3 grunthal, 2 cloacæ.
.
After 1 hour's exposure showed
... 9 cloacæ, 1 grunthal.
„ 2 hours'
,,
,,
... 9 grunthal, 1 cloacæ.
„ 5 hours'
,,
,,
... 5 coscoroba, 2 grunthal, 3 cloacæ.
Subsequent samples proved sterile at noon on second day.
Note.—The final analysis showing such a large mixture of organisms is probably due to the inclusion
,
of a particle of faecal matter in the last sample ; it will be observed that in the samples previous to this
there was a decided tendency for bacillus grunthal to predominate over the others as the exposure
continued.
On two separate occasions mixtures containing the ordinary organisms in cowdung
became sterile under one hour.
Experiment No. 23—Cowdung.
,
In order to provide a larger number of varieties a mixture of 10 samples of
cowdung were taken and the emulsion was exposed in the ordinary way.
Emulsion before exposure contained.
l coli communis, 2 coscoroba, 4 grunthal,
1 cloacæ, 1 acidi lactici.
After 1 hour's exposure showed
... 5 grunthal, 1 neapolitanus, 3 No. 101, No. 6.
, ,
2 hours'
,,
........... 4 grunthal, 1 lactis æmrogenes, 2 cloacæ), 1
neapolitanus, 1 coscoroba, 1 No. 67.
3
, ,
3
,,
,,
,,
... 3 grunthal, 1 coscoroba, 2 lactis æxrogenes, 2
No. 67, 1 No. 101, 1 No. 6.
Noon on the second day showed
... 5 cloacæ, 3 lactis ærogenes, 2 No. 101.
At 3 on the second day10 No. 67.
At 5 on the second day
,,
... 10 cloaca.
Noon of the third day and subsequent samples were sterile in 20 cc.
Note.—Coli communis did not survive after one hour's exposure ; bacillus grunthal did not in this
mixture persist till the end, but appears to have disappeared sometime prior to noon of the second day ;
bacillus No, 67 and bacillus cloacm both appeared to survive till quite late ; the rare organism No. 6
was present in this mixture and survived after three hours' exposure to sun.
.Experiment No. 24.
.
A similar mixture of 10 samples of cowdung were taken ; but in this case a layer
of sterile sand was placed at the bottom of the dish, so as to more nearly represent
the conditions obtaining in nature, and to reduce the power of the rays of the sun.
Emulsion before exposure contained.
5 coscoroba, 4 grunthal, 1 acidi lactici.
After 1 hour's exposure showed
... 5 coscoroba, 3 grunthal, 1 acidi lactici, 1 No. 67.
,,
2 hours
,,...
1 coli communis, 2 coscoroba, 3 grunthal, 1
lactis æmrogenes, 3 cloacæ.
3
)1
... 5 grunthal, 1 coscoroba, 1 cloacæ, 3 No. 67.
, ,
, ,
7 3
5
, ,
,,
,,
,,
... 6 coscoroba, 4 grunthal.
Evening of the second day (that is 7 neapolitanus, 1 coscoroba, 2 grunthal.
after 17 hours' exposure) showed.
Evening of the third day and subsequent samples proved sterile.
Note.—Coli survived after two hours' exposure, but not more. Appearance and
disappearance of both lactis ænrogenes and No. 67. Samples were not taken with sufficient frequency
towards the end of the experiment ; hence the last surviving organism was not known.
.
.