NationalLibraryOfScotland/medical-history-of-british-india huggingface dataset:
122
RINDERPEST.
TABLE OF RESULTS.-EXPERIMENT NO. 3-D.
SERUM No. 3-D.
METHOD OF PREPARING.—Two injections of DEFIBRINATED
V. B.
Reaction of Controls from which Blood was taken.
No. of
Bulls.
115
120
2
356
362
360
381
361
387
Temperature
reaction.
Slight
High
High
High
„
„
„
„
„
40.0 Max.
40.8
„
Lesions.
Vesicles.
Nil.
8th day
Ulcers.
Nil.
„
Result.
Recovery.
„
Reaction of Serum Making Bulls.
40.9 Max.
Nil.
Nil.
Recovery.
Reaction of Serum Testing Animals.
40.7 Max.
40.8
„
41.0
„
41.0
„
41.1
„
411
„
Nil.
10th day
9th „
7th „
8th „
10th „
Nil
11th day
10th „
8th „
9th „
11th „
Recovery.
„
„
Died 12th
day.
Recovery.
Died 12th
day.
Remarks.
Dose per 600 lbs.
body weight.
10 c.c. ditto.
10 c.c. ditto.
5 c.c. ditto.
5 c.c. ditto.
2 c.c. ditto.
2 c.c. ditto.
Comparisons of the results of testings of the different
serums:—
Serum
No.
2.A
1-A
3-A
How prepared.
One injection of V. B. plus Pot. Cit.
during period of attack (1,500 c.c.
V. B. plus 1,500 c.c. Pot. Cit. 0.5%.)
Ditto.
One injection of defibrinated V. B.
during attack (1,500 c.c. defibrinat-
ed V. B.)
Animals
used.
Buffaloes.
Plains
bulls.
Plains
bulls.
Protective dose.
1 c.c. per 600 lbs. body
weight. Plains animal.
4 c.c.
ditto.
7 c.c.
ditto.
RINDERPEST.
123
Serum
No.
X
1-B
1-B2
V
1-D
3-D
1-C
How prepared.
One injection of V. B. plus Pot. Cit. 6
days after recovery from attack
(1,500 c. c. V. B. plus 1.500 c.c. Pot.
Cit. 0.5%).
Ditto.
One injection of V. B. plus Pot. Cit.
12 days after recovery from attack
(1,500 c.c. V. B. plus 1,500 c.c. Pot.
Cit. 0.5%).
Two injections of V. B. plus Pot.
Cit. 1st, 6 days after recovery from
attack (1,500 c.c. V. B. 1,500 c.c.
Pot. Cit. 0.5%); 2nd, 16 days after
1st injection) 3,000 c.c. V. B. plus
1,000 c.c. Pot. Cit. 1.5%).
Ditto.
Two injections defibrinated blood
alone, 1st, 6 days after recovery
from attack (1,500 c.c. defibrinated
V. B.); 2nd, 25 days after 1st injec-
tion (3,000 c.c. defibrinated V. B.)
Two injections of V. B. plus Pot, Cit.
1st, injection during period of at-
tack (1,500 c.c. V. B. plus 1,500 c.c.
Pot, Cit. 0.5%); 2nd, 16 days after
1st injection) 3,000 c.c. V. B. plus
1,500 cc. Pot. Cit. 1.5%).
Animal
used.
Buffaloes.
Plains
bulls.
Ditto.
Buffaloes.
Plains
bulls.
Ditto.
Ditto.
Protective close.
2 c.c. per
weight.
10 c.c.
12 c.c.
4 c.c.
12 c.c.
12 c.c.
6 c.c.
600 lbs. body
Plains animal.
ditto.
ditto.
ditto.
ditto.
ditto.
ditto.
N.B.—V. B.—Virulent Blood.
Pot. Cit.—Potassium Citrate.
GENERAL CONCLUSIONS drawn from the results of the
serum preparation experiments.
The following conclusions are based on the results of the
experiments recorded in this report. It must be understood
that in the preparation and testing of anti-Rinderpest serum
absolute accuracy and uniformity of results cannot always be
obtained. In each series of experiments, both in preparing
and testing its potency, the factors vary to such an extent that
only relative accuracy can be arrived at.
Bulls of like age, condition, and breed do not always give
a serum of like potency. For some causes, not understood,
certain bulls afford very weak serum, and it is one of the diffi-
culties in preparing brews of Rinderpest serum that there is
no method except actual tests in living animals of deciding the
124
RINDERPEST.
value of serum from individual animals before mixing them
together. The method of standardising the serum by ascer-
taining on a series of living animals the dose necessary to pro-
tect a bull of Hill breed per 600 lbs. body weight, although a
good practical test of the relative value of the serum is a very
inaccurate measure of the immune units present in the serum.
Unfortunately no other method for standardising Rinderpest
serum has yet been discovered.
(1) The serum resulting from a single massive injection
of virulent blood is superior in potency to that prepared by two
successive injections of virulent blood.
All these sera were tested on Hill cattle of maximum sus-
ceptibility and the dose stated for Plains cattle per 600 lbs.
body weight is estimated on the supposition that these latter
animals require for protection only an eighteenth of the dose
necessary for Hill bulls :—
1 injection at a negative phase (buffalo) serum ..
1
„
„
„
(bull) „ „
1
„
followed by a 2nd injec-
tion at a positive phase
..
.
1
„
at a positive phase (buffalo) serum ..
1
„
„
„
(bull)
„
..
2
„
„
„
(buffalo) „
..
2
„
„
„
(bull)
„
..
1 c.c.
4 c.c.
6 c.c
2 c.c.
7 c.c.
4 c.c.
12 c.c.
dose per 600 lbs. B. W. (Plains
cattle).
ditto.
ditto.
ditto.
ditto.
ditto.
ditto.
The results of these experiments support the view expressed
on page 104 that a second massive injection of virus given at a
period when a large quantity of anti-bodies produced by the
first massive injection are present in the animal body, exercises
a direct detrimental effect on the potency of the serum. If
we find that this method when applied to the routine prepara-
tion of anti-Rinderpest serum in Hill bulls gives a similar re-
sult, considerably less virus will be required and consequently
a large saving in the number of Hill bulls will be effected. In
our present system, Hill bulls are prepared for serum by two
injections, the first varies from about 1,200 c.c. to 2,000 c.c.,
the second injection from 1,500 to 3,000 c.c. A single injection
only of from 1,500 to 2,000 would be necessary by the new
RINDERPEST.
125
method. At present the bulls used for serum preparation require
a period of over 9 weeks before they, can be bled for serum. By
using a single injection only a period of three weeks would be
saved. It is proposed to apply this system tentatively in the
routine work of serum preparation at Muktesar.
(2) The serum resulting from a single massive injection
of virulent blood given at a negative phase is superior to that
prepared by a single massive injection at a positive phase.—
1 injection at a negative phase (buffalo) serum .. 1 c.c.
1
„
„
positive
„
(do.)
„ .. 2 c.c.
1
„
„
negative „
(bull)
„ .. 4 c.c.
1
„
„
positive
„
(do.)
„ .. 7 c.c.
per 600 lbs. B. W. (Plains cattle).
ditto.
ditto.
ditto.
The result has a most important bearing on the subject of
serum preparation. It suggests that the best time to administer
a massive injection of virus in the case of very susceptible
cattle as Hill bulls is immediately after the immunization
reaction has passed off and before sufficient time has elapsed for
the formation of anti-bodies. In the case of cattle of less
susceptibility, such as Plains bulls and buffaloes, the massive
injection can be super-imposed on the reaction from an inocula-
tion of virus without anti-serum.
The adoption of this method has been very successful in
the preparation of an anti-Rinderpest serum from Plains bulls
and buffaloes. Previous attempts to prepare a potent serum
by injecting Plains bulls and buffaloes with virus obtained
from Plains cattle were not successful. In these former experi-
ments one or more massive injections of virus were given at
positive periods, namely, when the animal had completely re-
covered from the reaction of an immunization process or a first
injection. In our present experiments the massive injection of
virus was given during an actual attack of Rinderpest at a
period regulated according to the severity of the attack.
(3) The serum from a massive injection of virulent blood
diluted in an equal volume of Citrate of Potash solution is su-
perior to that prepared by a massive injection of defibrinated
blood undiluted. By this method the virulent blood is taken
direct from the control animal, into a 0.5 per cent. solution of
126
RINDERPEST.
Citrate of Potash. This has several practical advantages over the
defibrinated method. It effects a saving of 10 per cent. to
15 per cent. in volume, also in the expenditure of time, labour
and glass, which is necessary in the defibrinating and straining
process. By the action of the hypotonic saline solution the red
blood cells are dissolved and the whole volume of the
fluid rendered more suitable for injections and absorption.
Theoretically the dissolution of the red cells liberates the
antigen which is consequently presented in a form more readily
acted on by the tissue cells. We have repeatedly observed that
the injection of fluid (virulent blood and peritoneal fluid)
mixed in Pot. Cit. solution is much more rapidly absorbed
than an equal volume of defibrinated blood and is attended
with less inconvenience to the injected animal. Rapidity of
absorption is undoubtedly a favourable factor in the prepara-
tion of a good serum.
(4) A serum of very high potency can be prepared from
Plains buffaloes by a single massive injection of virulent blood
obtained from buffalo controls. The virulent blood is diluted
in an equal volume of Pot. Cit. solution and is injected at a
negative phase, namely, during the actual period of an attack
of Rinderpest or immediately after.
1 injection at a negative phase (buffalo) serum—1 c.c.
1
„
positive
„
(do.)
„
2 c.c.
2 injections
„
„
(do.)
„ 4 c.c.
per 600 lbs. B. W. (Plains cattle).
ditto.
ditto.
The result of this experiment appears to go far towards
solving the difficulties connected with the shortage of the supply
of highly susceptible cattle of Hill breed and the question of
the future supply of anti-Rinderpest serum. In all our past
operations a large supply of Hill bulls was necessary to supply
virus for the injection of all serum preparing cattle both of Hill
and Plains breed. It was considered that the virus obtained
from Plains cattle (bulls and buffaloes) was not of sufficient
virulence to produce a potent serum. It was also understood
that the serum obtained from Hill cattle was far more potent
than it was possible to produce from serum preparing animals
RINDERPEST.
127
of Plains breed. The serum prepared in these experiments
by the exclusive use of Plains buffaloes is equal in potency to
serum derived from the exclusive use of cattle of Hill breed.
Consequently, Plains buffaloes can be to a large extent used in
the place of Hill cattle. They are cheap, and can be obtained
in sufficient number, also few die during the process of bleeding
and serum preparation.
Owners do not object to selling buffaloes for Laboratory
purposes. By the mixture of serum prepared by this method
from Plains buffaloes with the serum prepared in like manner
from Plains bulls, I consider that brews of Rinderpest anti-
serum can be manufactured of a potency equal to that of the
Muktesar serum. A serum of good practical value can be pre-
pared from Plains bulls by a single massive injection of viru-
lent blood obtained from Plains bull controls. The virulent
blood is diluted in an equal volume of Pot. Cit. solution and
is injected at a negative phase, namely, during the actual period
of an attack of Rinderpest or immediately after.
The serum prepared from Plains cattle is not so potent
as that from Plains buffaloes. It is, however, as strong as
that obtained from Plains cattle injected with virus from Hill
bulls by our present routine method. The Plains cattle used
for this experiment were inexpensive animals of ordinary vil-
lage type. They were only slightly susceptible to Rinderpest
and few died during the process of serum preparation. In all
these experiments the bleedings for serum were made at a period
of about fourteen days following the massive injection. The
satisfactory results show that the anti-bodies are present at
that time.
Under our present routine a period of three weeks at least
is allowed from last injection. In further operations this pe-
riod can be curtailed to two weeks.
If the practical application of these methods meets with
the same success in the routine serum making preparation at
Muktesar, a large number of Plains buffaloes can be used to
supply virus and a lesser number of Hill bulls will be required.
128
RINDERPEST.
This would enable the serum operations at Muktesar to continue
to the same extent as in the past without danger of failure
from lack of sufficient numbers of Hill cattle. At the Bareilly
Branch Laboratory if the grounds were extended and more
accommodation provided, serum preparation could be carried
on for six months (October to March) each year, and about two
to three lacs doses could be obtained from this source.
SERUM NO. 2-A.
CONTROLS.
Buffalo No. 72.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
3rd day
7th „
8th „
.. 40.8º C.
.. 40.4º C. Diarrhœa.
.. Bled 2,000 c.c. Died
after being bled.
Buffalo No. 74.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
4th day
6th & 7th day
8th day
.. 40.5º C.
.. 40.4º C.
.. Bled 2,000 c.c.
Buffalo No. 80.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
3rd & 4th day
5th—7th „
8th day
.. 40.5º C.
.. 40.0º C.
.. Bled 2,000 c.c.
Buffalo No. 81.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
4th day
7th „
8th „
.. 40.6º C.
.. 41.0º C.
.. Bled 1,000 c.c.
Buffalo No. 85.—Inoculated with 1 c.c. defibrinated V, B.
from Bull No. 28.
4th & 7th day
8th day
.. 40.4º C.
.. Bled 1,000 c.
RINDERPEST.
129
SERUM MAKING.
Buffalo No. 73.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
6th day
7th „
8th „
10th „
24th „
.. 40.3º C.
.. 40.5º C.
.. Inoculated with 1,500 c.c. V. B.+1,500 c.c. Pot.
Cit. 0.5%.
.. 40.0º C.
.. Bled 4,500 c.c.
Buffalo No. 75.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
3rd day
4th „
5th & 6th day
8th day
12th day
15th „
.. 40.0º C.
.. 40.3º C.
.. 40.0º C.
.. Inoculated with 1,500 c.c. V. B. +1,500 c.c. Pot.
Cit. 0.5%.
.. 39.9º C.
.. 39.1º C. Died at night.
Buffalo No. 76.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
6th day
7th „
8th „
11th day
24th „
.. 40.1º C.
.. 40.4º C.
.. Inoculated with 1,500 c.c. V. B. +1,500 c.c. Pot.
Cit. 0.5%.
.. 39.8º C.
.. Bled 4,000 c.c.
Buffalo No. 77.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
7th day
8th „
11th „
24th „
.. 39.9º C.
.. Inoculated with 1,500 c.c. V. B.+1,500 c.c. Pot.
Cit. 0.5%.
.. 40.0º C.
.. Bled 4,000 c.c.
Buffalo No. 78.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
6th day
7th „
8th „
9th—16th day
24th day
.. 401º C.
.. 40.3º C.
.. Inoculated with 1,500 c.c. V. B.+1,500 c.c. Pot.
Cit. 0.5%.
.. 39.7º C.
.. Bled 3,500 c.c.
Buffalo No. 84.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
5th day
6th „
.. 40.1º C.
40.3º C.
9
130
RINDERPEST.
7th day
8th „
9th „
10th „
11th „
12th „
13th „
24th „
.. 40.6º C.
.. Inoculated with 1.500 c.c. V. B.+1,500 c.c. Pot.
Cit. 0.5%.
.. 40.0º C.
.. 40.4º C.
.. 40.0º C.
.. 40.2º C.
.. 40.0º C.
.. Bled 4,000 c.c.
SERUM TESTING.
Bull No. 269 @ 10 c.c.—Inoculated with 90.21 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 31.
17th day
.. Discontinued.
Bull No. 281 @ 10 c.c.—Inoculated with 88.20 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 31.
17th day
.. Discontinued.
Bull No. 267 @ 5 c.c.—Inoculated with 36.45 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 31.
17th day
.. Discontinued.
Bull No. 271 @ 5 c.c.—Inoculated with 36.45 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 31.
17th day
.. Discontinued.
Bull No. 277 @ 2 c.c.—Inoculated with 12.66 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 259.
15th day
.. Discontinued.
Bull No. 266 @ 2 c.c.—Inoculated with 10.92 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 259.
15th day
.. Discontinued.
Bull No. 274 @ 2 c.c.—Inoculated with 8.28 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 259.
15th day
.. Discontinued.
Buffalo No. 153 @ 10 c.c.—Inoculated with 134.10 c.c.
serum + 1 c.c. defibrinated V. B. from Bull No. 31.
17th day
.. Discontinued.
Buffalo No. 150 @ 10 c.c.—Inoculated with 131.10 c.c.
serum + 1 c.c. defibrinated V. B. from Bull No. 31.
17th day
.. Discontinued.
RINDERPEST.
131
Buffalo No. 155 @ 5 c.c.—Inoculated with 63.51 c.c.
serum + 1 c.c. defibrinated V. B. from Bull No. 31.
17th day
.. Discontinued.
Buffalo No. 154 @ 5 c.c.—Inoculated with 56.25 c.c.
serum + 1 c.c. defibrinated V. B. from Bull No. 31.
17th day
.. Discontinued.
"SERUM No. X."
CONTROLS.
Buffalo No. 118.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 33.
5th day
8th „
.. 39.6º C.
.. Bled 2,000 c.c.
Buffalo No. 119.—Inoculated with 1 c.c. defibrinated
V. B. from Bull No. 33.
4th & 5th day
8th day
.. 40.0º C.
.. Bled 1,000 c.c.
Buffalo No. 120.—Inoculated with 1 c.c. defibrinated
V. B. from Bull No. 33.
5th day
8th „
.. 40.0º C.
.. Bled 2,000 c.c.
SERUM MAKING.
Buffalo No. 22.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
6th day]
14th „
15th „
16th „
27th & 28th day
33rd day
.. 38.9º C. Fæces soft.
.. Inoculated with 1,500 c.c. V. B.+1,500 c.c. Pot.
Cit. 0.5%.
.. 39.7º C.
.. 39.5º C.
.. 39.5º C.
.. Bled 4,500 c.c.
Buffalo No. 79.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
3rd & 4th day
5th & 6th „
7th day
14th „
15th „
28th „
.. 40.0 º C.
.. 40.6º C.
.. 40.0º C.
.. Inoculated with 1,500 c.c. V. B.+1,500 c.c. Pot.
Cit. 0.5%.
.. 40.6º C.
.. Bled 4,000 c.c.
132
RINRERPEST.
Buffalo No. 83.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
7th & 8th day
14th day
15th „
28th „
.. 40.0º C.
.. Inoculated with 1,500 c.c. V. B.+1,500 c.c. Pot.
Cit. 0.5%;
.. 39.2º C.
.. Bled 4,000 c.c.
SERUM TESTING.
Bull No. 283 @ 10 c.c.—Inoculated with 99 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 31.
18th day
.. Discontinued.
Bull No. 276 @ 10 c.c.—Inoculated with 90.21 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 31.
Bull No. 278 @ 5 c.c.—Inoculated with 41.25 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 31.
18th day
.. Discontinued.
Bull No. 268 @ 5 c.c.—Inoculated with 37.35 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 31.
18th day
.. Discontinued.
Bull No. 282 @ 2 c.c.—Inoculated with 12.06 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 259.
4th day
7th „
18th „
.. 40.0º C.
.. 40.0º C.
.. Discontinued.
Bull No. 37 @ 2 c.c.—Inoculated with 11.76 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 259.
6th day
7th „
8th „
9th „
10th „
11th „
12th „
13th „
18th „
.. 40.6º C.
.. 40.7º C.
.. 40.6º C.
.. 40.6º C. Vesicles.
.. 39.4º C. Vesicles.
.. 39.3º C. Ulcers.
.. 39.6º C. Ulcers nearly healed up.
.. 39.4º C. Ulcers healed up.
.. Discontinued.
Buffalo No. 152 @ 10 c.c.—Inoculated with 142.80 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 31.
17th day
.. Discontinued.
RINDERPEST.
133
Buffalo No. 156 @ 10 c.c.—Inoculated with 141.00 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 31.
17th day
.. Discontinued.
Buffalo No. 149 @ 5 c.c.—Inoculated with 64.50 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 31.
17th day
.. Discontinued.
Buffalo No. 151 @ 5 c.c.—Inoculated with 64.20 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 31.
17th day
.. Discontinued.
"SERUM No. Y."
CONTROLS.
Buffalo No. 120.—Inoculated with 1 c.c. defibrinated
V. B. from Bull No. 33.
5th day
8th „
.. 40.0º C.
.. Bled 2,000 c.c.
Buffalo No. 121.—Inoculated with 1 c.c. defibrinated
V. B. from Bull No. 33.
5th day
6th & 7th day
8th day
.. 40.1º C.
.. 40.3º C.
.. Bled 2,000 c.c.
Buffalo No. 122.—Inoculated with 1 c.c. defibrinated
V. B. from Bull No. 33.
7th day
8th „
.. 40.0º C.
.. Bled 2,000 c.c.
Buffalo No. 146.—Inoculated with 1 c.c. defibrinated
V. B. from Bull No. 51.
5th & 6th day
7th day
8th „
.. 40.3º C.
.. 40.7º C.
.. Bled 2000 c.c.
Buffalo No. 147.—Inoculated with 1 c.c. defibrinated
V. B. from Bull No. 51.
6th day
7th „
8th „
.. 40.2º C.
.. 40.4º C.
.. Bled 1,000 c.c.
134
RINDERPEST.
Buffalo No. 148.—Inoculated with 1 c.c. defibrinated
V. B. from Bull No. 51.
5th day
6th „
7th „
8th „
.. 40.4º C.
.. 41.0º C.
.. 40.8º C.
.. Bled 2,000 c.c. Vesicles.
SERUM MAKING.
Buffalo No. 82.—Inoculated with 1 c.c. defibrinated
V. B. from Bull No. 28.
3rd & 4th day
8th day
9th „
10th „
11th „
14th „
15th „
16th „
17th „
.. 40.3º C.
.. 39.3º C. Vesicles.
.. 38.7º C. Vesicles and Ulcers.
.. 39.1º C. Ulcers healing.
.. 39.2º C. Ulcers healed up.
.. Inoculated with 1,500 c.c. V. B.+1,500 c.c. Pot.
Cit. 0.5%.
.. 39.7º C.
.. 38.8º C.
.. 36.0º C. Fæces soft. Died at night.
Buffalo No. 88.-Inoculated with 1 c.c. defibrinated
V. B. from Bull No. 28.
3rd & 4th day
5th day
7th „
14th „
15th „
30th „
32nd—34th day
45th day
.. 40.1º C.
.. 40.0º C. Fæces soft.
.. 40.5º C.
.. Inoculated with 1,500 c.c. V. B.+1,500 c.c. Pot.
Cit. 0.5%.
.. 39.2º C.
.. Inoculated with 2,000 c.c. V. B.+1,000 c.c. Pot.
Cit. 1.5%.
.. 39.4º C.
.. Bled 4,000 c.c.
Buffalo No. 89.—Inoculated with 1 c.c. defibrinated
V. B. from Bull No. 28.
3rd day
4th „
7th „
10th & 11th day
14th day
15th „
30th „
31st „
35th „
45th „
.. 40.0º C.
.. 40.2º C.
.. 40.0º C.
.. 39.0º C. Diarrhœa.
.. Inoculated with 1,500 c.c. V. B.+1,500 c.c. Pot.
Cit. 0.5%.
.. 39.9º C.
.. Inoculated with 3,000 c.c. V. B.+1,500 c.c. Pot.
Cit. 1.5%.
.. 39.1º C.
.. 40.0º C.
.. Bled 5,000 c.c.
RINDERPEST.
135
SERUM TESTING.
Bull No. 307 @ 10 c.c.—Inoculated with 76.80 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 67.
4th day
5th „
7th „
18th „
.. 40.4º C.
.. 40.6º C.
.. 39.6º C. Diarrhœa.
.. Discontinued.
Bull No. 338 @ 10 c.c.—Inoculated with 67.80 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 67.
3rd & 6th day
18th day
.. 40.6º C.
.. Discontinued.
Bull No. 308 @ 5 c.c.—Inoculated with 31.35 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 67.
4th day
5th & 6th day
7th day
18th „
.. 40.4º C.
.. 40.0º C.
.. 40.4º C.
.. Discontinued.
Bull No. 354 @ 5 c.c.—Inoculated with 28.50 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 67.
3rd—6th day
18th day
.. 40.0º C.
.. Discontinued.
Bull No. 314 @ 2 c.c.—Inoculated with 10.20 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 67.
3rd day
4th „
5th „
7th „
8th „
9th „
10th „
11th ,
12th „
13th „
.. 40.0º C.
.. 40.8º C.
.. 40.6º C.
.. 38.6º C. Diarrhœa. Dull.
.. 39.5º C. „ „
.. 39.3º C.
„ Weak.
.. 39.0º C. Fæces soft.
.. 37.0º C. Diarrhœa. Weak.
.. 35.0º C. „ „
.. 35.0º C.
„
„ Died at night.
Bull No. 336 @ 2 c.c.—Inoculated with 9.54 c.c.
serum + 0.5 c.c. defibrinated V. B. from Bull No. 67.
4th day
5th „
6th „
7th „
8th „
9th „
10th „
11th „
.. 40.3º C.
.. 41.0º C.
.. 40.3º C.
.. 40.4º C. Diarrlœa.
.. 41.0º C.
„
Vesicles. Ulcers.
.. 40.4º C.
„
Ulcera.
.. 38.3º C. Diarrhœa. Ulcers persist.
.. 35.0º C. „
„
„ Died at night.
136
RINDERPEST.
"SERUM No. 1-A."
CONTROLS.
Bull No. 9.—Inoculated with 1 c.c. defibrinated Y. B.
from Bull No. 28.
3rd day
4th „
7th „
8th „
.. 40.2º C.
.. 40.0º C. Fæces soft.
.. 40.0º C.
.. Bled 2,000 c.c. Vesicles.
Bull No. 13.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
4th day
5th „
6th „
7th „
8th „
.. 40.1º C.
.. 40.4º C.
.. 40.5º C.
.. 40.4º C. Vesicles.
.. Bled 2,000 c.c. Vesicles. Fæces soft.
Bull No. 92.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
4th & 5th day
6th „
7th „
8th „
.. 40.0º C.
.. 40.0º C. Fæces soft.
.. 40.2º C.
.. Bled 2,000 c.c. Vesicles.
SERUM MAKING.
Bull No. 102.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
4th day
5th—7th day
8th day
15th „
16th & 17th day
18th day
30th „
.. 40.6º C. Fæces soft.
.. 40.3º C.
.. Inoculated with 1,500 c.c. V. B.+1,500 c.c. Pot Cit.
0.5%.
.. 40.0º C.
.. 40.4º C.
.. 40.0º C.
.. Bled 3,500 c.c.
Bull No. 103.—Inoculated with 1 c.c. defibrinated V. B.
from Bull No. 28.
4th day
6th „
7th „
8th „
11th „
12th „
13th „
14th „
.. 40.0º C. Fæces soft.
.. 40.3º C.
.. 40.5º C.
.. Inoculated with 1,500 c.c. V. B.+1500 c.c. Pot. Cit.
0.5%.
.. 40.1º C.
.. 40.2º C.
.. 40.1º C.
.. 40.6º C.