104
7th June.—The muco-purulent discharge has stopped and a clear fluid now drops. Left sub-maxillary gland
reduced in size.
8th.—
9th.—
10th.—
11th.—
Temperature
„
„
„
„
A.M. 39.2°C.
39.3°C.
36.7°C.
37.4°C.
36.9°C.
P.M. 39.6°C.
„ 39.3°C.
„ 38.4°C.
„ 37.3°C.
„ 36.6°C.
Hæmatozoa
„
„
„
„
numerous.
few
absent.
very numerous.
swarming.
At 6-45 P.M., the animal became much weaker, and death took place at 4 A.M. on the 12th instant.
Autopsy.—At 10 A.M., 6 hours post mortem.
Body fairly nourished ; considerable œdema of the most dependent part of the abdominal walls. No pleuritic
adhesions.
Lungs.—Light pink in colour. The apices are the seat of emphysema, as also the free edges of the middle lobes.
On section, the tissues are a peculiar orange colour, and on pressure a considerable amount of frothy mucus exudes from
the cut surfaces.
The pericardial sac contains a few ounces of a deep yellow-coloured fluid.
Heart.—Weight 4 lbs. 3 ozs. There are a few small circular ecchymoses at the base, lying under the visceral
pericardium. On section, there are no sub-endocardial extravasations in any of the cavities ; the valves are healthy.
The left ventricular walls are hypertrophied.
Liver.—Large, but healthy. On section, much fluid blood exudes from the cut surfaces.
Kidneys.—Right weighs 14.5 ozs. Left 13.5 ozs. The capsules strip easily ; the organs are pale, but healthy.
Spleen.—Weight 1 lb. 15 ozs. Firm, and presents a few small petechiæ on its surfaces.
Stomach.— On section, there is a bright yellow line between cuticular and villous portions, but otherwise the only
abnormal feature was the presence of a few reddish coloured patches on the mucous surface of the villous portion.
No ecchymoses or parasites.
Intestines.—Healthy.
HORSE No. XII.
Subcutaneous inoculation on two occasions with soiled rat's blood ; results negative. Fifty-two days after the
second injection, inoculation with one minim of soiled Horse-Surra blood. Period of incubation six days. Death on
the 15th day of disease.
Bay, country-bred gelding, aged, in fair condition, and healthy.
21st January 1891.—The animal was inoculated subcutaneously on each side of the neck with 1.0 c.c. of blood,
taken from the hearts of two rats (Mus decumanus) immediately after death ; the injected blood contained numerous
hæmatozoa. Temperature 2.30 P.M. 36.9°C. 5 P.M. 37.2°C.
22nd.—There is slight swelling at the seat of the inoculation ; appetite good, evacuations normal.
Temperature A.M. 36.8°C. No hæmatozoa observed. Temperature P.M. 37.6°C.
23rd.—The swelling and œdema have increased at the seat of the inoculation, and the part is hot to the touch.
Temperature A.M. 37.3°C. No hæmatozoa observed. Temperature P.M. 38.1°C.
24th.—The swelling and œdema have increased since yesterday, and the tumour is tense and tender on manipula-
tion. Appetite good ; visible mucous membranes normal, evacuations natural. Temperature A.M. 37.7°C. No hæma-
tozoa observed. Temperature P.M. 38°C.
25th.—Condition unchanged. Temperature A.M. 37.5°C. NO hæmatozoa discovered. Temperature P.M. 38°C.
26th.—The swelling remains the same size as on the 24th. Temperature A.M. 38°C. No hæmatozoa observed.
Temperature P.M. 38.5°C.
From the 27th January to February 11th, the animal appeared in good health, the temperature remained normal
as seen in the chart, and no organisms were discovered in the blood. On the 12th the animal was re-inoculated subcu-
taneously over the right scapular region, with 2.0 c.c. of rat's (MUS decumanus) blood containing very numerous
hæmatozoa. The rat from which this blood was taken had an enlarged spleen, and on section of the stomach, some
petechiæ were observed near the pylorus. The horse's allowance of corn to be discontinued. Temperature A.M.37.6°
C. P.M. 37.8°C.
13th February 1891.—There is considerale swelling and œdema at the seat of yesterday's inoculation. Temperature
A.M. 37.5°C. No. hæmatozoa observed. Temperature P.M. 37.8°C.
14th.—The swelling and œdema at the seat of the inoculation have become more extended. The animal appears
healthy. Temperature A.M. 37.3°C. No hæmatozoa observed. Temperature P.M. 37.6°C.
15th.—Swelling and œdema still extending ; appetite good. Temperature A.M. 37.2°C. No hæmatozoa observed
Temperature P.M. 37.5°C.
16th.—Swelling and œdema stationary. Temperature A.M. 37.7°C. No hæmatozoa observed.
Temperature
P.M. 37.6 C.
On the 20th February petechiæ appeared at the inner canthus of both eyes, especially the left, while on the
22nd they were to be seen chiefly on the membranes of the right eye. On the 22nd they were well marked in both
eyes, also on the septum of the left nostril, and on March 1st, extensive petechiæ appeared at the upper and inner
border of the right cornea, lying on the sclerotic coat under the conjunctiva.
From the 2nd March to the 5th April the animal appeared in good health, the temperature continued normal,
and no hæmatozoa were observed in the blood.
In consequence of no symptoms having supervened since the inoculation. with rat's blood, the horse was this
morning, 5th April, inoculated on the muzzle, with one minim of blood containing numerous hæmatozoa taken from
Horse No. XIX.