60
After 2 hours'exposure showed.
2 cloacae, 3 No. 67, 2 coseoroba, 1 neapolitanus,
1 grunthal, 1 No. 101.
3
,,
4 grunthal, 1 acidi lactici, 1 cloacm, 1 lactis.
æærogenes, 1 coscoroba, 1 No. 67, 1 No. 101.
2 coscoroba, 1 acidi lactici, 2 No. 67, 5 No. 101.
, ,
Noon of second day
... 4 cloaca, 4 grunthal, 1 acidi lactici, 1 cos-
'
coroba.
Evening of second day
„
7 grunthal, 2 acidi lactici, 1 coscoroba.
Evening of third day
2 acidi lactici, 8 grunthal.
Evening of fourth day
... 10 grunthal.
Evening of fifth day
,,
. . 10 grunthal.
Subsequent samples proved sterile in 20 cc,
Note.—The usual appearance and disappearance of organisms ; bacillus gruntlhal survived till
the end.
The following experiments have been carried. out with human feces. These
results have been obtained from experiments carried on under identical conditions
with those already reported., with this single exception, that on account of the
large amount of mucus present in human feces, it has been found. necessary to filter
the emulsion through cotton wool. Several results obtained without this precaution
are entirely worthless, because bacilli enveloped. in mucus do not behave in the same
way as those in water :—
Experiment No. 29.
Emulsion before exposure contained.
5 acidi lactici, 1 coseoroba, 4 grunthal,
After 1 hour's exposure showed
... 1 coli communis, 6 grunthal, 1 acidi lactici, 2
No. 67,
2 hours'
2 grunthal, 2 lactis ærogenes, 4 No 67, 1 No.
, ,
101, 1 cloacae.
Sample taken on the noon of the 6 No 67, 4 grunthal.
second day (after 8 hours' exposure)
showed.
At 2 o'clock on the second day showed 10 grunthal.
At 5 o'clock on the second. day
„
S grunthal, 2 coseoroba.
Noon of the third. day showed
10 grunthal.
Evening of the third. day showed. ... No further organisms in 20 cc.
Note.—Coli communis survived one hour but not two the persistence and eventual disappearance
of organism No. 67 ; appearance and disappearance of lactis æogones, appearance of cloacæ, and
the final persistence of bacillus grunthal.
Experiment N o. 30.
Emulsion before exposure contained.
8 grunthal, 1 coli communis, 1 acidi lactici.
After 1 hour's exposure showed
... 5 lactis æærogenes, 4 acidi lactici, 1 No. 67.
„ 2 hours'
„
3 cloacae, 6 acidi lactici, 1 No. 101.
3 ,,
1 grunthal, 9 acidi lactici.
Noon of the second day „
... No fecal organism in 20 cc.
communis survived only one hour ; appearance and. disappearance of lactis æærogenes
persistence of acidi lactici. These results cannot be looked upon as satisfactory for want of more
frequent sampling. No samples were taken between the third hour and the noon of the second day ;:
the consequence is it is impossible to be certain as to which organism survived till the very end.
Experiment No. 31.
A mixture of five samples of human feces
Emulsion before exposure contained.
1 coscoroba, 9 grunthal.
After 1 hour's exposure showed
...
4 coli communis, 2 grunthal, 1 lactiscerogenes,.
2 neapolitanus, 1 No. 101.
2 1/4
hours
,,
4 cloaca', 3 coscoroba, 1 lactis æærogenes, 1
,,
...
acidi lactici.
3 1/4 ,, ,, ,, 8 cloacae,2 eoscoroba.
4 1/2 cloacae,2 eoscoroba, 1 grunthal.
,,
, ,
2
Noon of second. day showed (after 10
1 cloaca, 1 eoscoroba, 1 No. 6, 7 grunthal.
hours' exposure).
At 5 on the second day showed
... 10 grunthal.
Subsequent samples proved sterile in 20 cc.
Note.—The prevalence of grunthal to commence with ; appearance of 'coli communis after 1 hour's
exposure and disappearance entirely before 2 hours ; usual appearance of cloacae and great predomi-
nance until near the end when bacillus grunthal survived all others ; important comments are made
on this result in the succeeding part of this Chapter.