106
HORSE No. XLVIII.
Subcutaneous inoculation on two occasions with soiled rat's (Mus decumanus) blood. Results negative.
Inoculation with Horse-Surra blood 13 days after second inoculation. Period of incubation 10 days. Death on the
2nd day of disease.
White country-bred gelding, aged, in fair condition.
1891, October
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9th.—Temperature A.M.
10th.
11th.
12th.
13th.
14th.
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37.0°C.
37.2°C.
37.5°C.
37.5°C.
37.4°C.
37.4°C.
P.M. 37.4°C.
„ 37.5°C.
„ 38.2°C.
„ 38.2°C.
„ 38.2°C.
„ 38.4°C.
Blood healthy.
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15th.—Temperature A.M. 37.8°C. Blood healthy. At 4 P.M. inoculated the animal subcutaneously over the left
scapular region with 0.75 c.c. of blood containing numerous hæmatozoa, taken from the heart of a rat (Mus decumanus)
immediately after death. Temperature 5 P.M. 38.4°C.
16th.—There are a few ecchymoses on the conjunctivæ, deep red in colour and of recent date. There is at the seat
of inoculation a hard, circumscribed swelling, ovoid in form, surrounded by œdema and measuring three inches in its
largest diameter, and two inches in its shortest, and raised to the extent of one inch above the surrounding tissues.
Temperature A.M. 38.1°C. NO hæmatozoa. Temperature 5 P.M. 38.1°C.
17th.—The size of the swelling is less than yesterday ; but the swelling is hard and tender to the touch. Several
small ecchymoses, bright red in colour, are to be seen on the conjunctivæ, especially the right. Temperature A.M.
37.3°C. No hæmatozoa. Temperature 1 P.M. 37.8°C. Temperature 5 P.M. 38.3°C.
18th.—The swelling has still further decreased in size, but is hard and circumscribed. Temperature A.M. 37.3°C.
No hæmatozoa. Temperature 1 P.M. 37.8°C. 5 P.M. 38°C.
19th.—The visible mucous membranes are tinged yellow this morning, but there are no ecchymoses. Temperature
A.M. 37.5°C. No hæmatozoa. Temperature 1 P.M. 38.2°C. 5 P.M. 38.1°C.
From the 20th October to the 30th the animal appeared in good health, the temperature was normal and no
hæmatozoa were observed in the blood ; consequently on the latter date at 5 P.M. the animal was re-inoculated subcuta-
neously over the scapular regions with 0.75 c.c. of blood, containing numerous hæmatozoa, taken from the hearts of two
rats (Mus decumanus), immediately after death.
31st.—The swelling on the left side measures 3"×2" and is raised ¾" above the general surface of the surrounding
skin. On the right side there is a large irregular-shaped swelling, measuring 3.5"×2.5", over the scapula. Tempera-
ture A.M. 37.2°C. No hæmatozoa. Temperature 1. P.M. 38°C., 5 P.M. 38.1°C.
November 1st.—The swelling on the left side is reduced in size, that on the right remaining the same as yesterday,
and is very tender on manipulation. Temperature A.M. 37.5°C. Hæmatozoa absent. Temperature 1 P.M. 38°C. 5 P.M.
38.3°C.
From the 2nd to the 4th instant the condition was unchanged.
5th.—The swelling on the right side continues large in size, and is tender. Temperature A.M. 38.1°C. No hæma-
tozoa. Temperature 1 P.M. 38°C., 5 P.M. 38.7°C.
6th.—Temperature A.M. 37.3°C., 1 P.M. 38.2°C., 5 P.M. 37.7°C. No hæmatozoa.
7th.
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38°C.
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38.2°C.
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38.3°C.
8th.—The swellings on both sides of the body have entirely disappeared. Temperature A.M. 38°C. No hæma-
tozoa. Temperature 1 P.M. 38.7°C., 5 P.M. 37.8°C.
From the 9th to the 12th, the condition was unchanged, and as no hæmatozoa were observed in the blood, the
animal on the 12th was inoculated subcutaneously over the left scapular region with 0.5c.c. of blood, containing no
hæmatozoa, taken from the jugular vein of Horse No. L.
From the 13th November until the 19th, there was no change in the condition of the animal, but on the 20th, a
slight thickening could be perceived at the seat of inoculation ; otherwise there was nothing to point out the situation.
Temperature A.M. 35.6°C. NO hæmatozoa. Temperature 1 P.M. 37.8°C., 5 P.M. 38.7°C.
21st.—Temperature A.M. 37°C. No hæmatozoa. Temperature 1 P.M. 40°C., 5 P.M. 39.6°C.
22nd.—Temperature A.M. 37°C. Hæmatozoa numerous. Temperature 1 P.M. 38.8°C., 5 P.M. 38.2°C. There is a
yellow tinge of the conjunctivæ, and of the mucous membranes generally. The membrana nictitans in each eye is the
seat of numerous recent dark-coloured ecchymoses.
The animal fell at 9 P.M. and death took place at 1 o'clock A.M. on the 23rd.
Autopsy at 9 A.M., 23rd November 1891.
Body not emaciated ; tissues stained yellow, no fluid in the pleural cavities, and no pleuritic adhesions.
Lungs.—The under-surface of the visceral pleuræ is dotted over with extravasations of blood. These are especially
noticeable and aggregated together along the superior borders of the organs.
On section, the extravasations are 1/16" in thickness, and do not appear to involve the lung substance.
Heart.—The base is covered by a layer of orange-coloured exudation, ½" in thickness, which also accompanies the
anterior and posterior coronary vessels in their course. The cavities of the organ are distended more or less with
clots, some yellow and some dark-red in colour.
Right Auricle.—Presents a few sub-endocardial ecchymoses.
Right Ventricle.—Presents very numerous and extensive sub-endocardial ecchymoses.